LOCUS       DATXQK010000001        94035 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.001, whole genome shotgun sequence.
ACCESSION   DATXQK010000001 DATXQK010000000
VERSION     DATXQK010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 94035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 94035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..94035
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.001"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            228..1463
                     /gene="arcA"
                     /locus_tag="VC394_000001"
     CDS             228..1463
                     /gene="arcA"
                     /locus_tag="VC394_000001"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEP5993507.1"
                     /translation="MTAQTPIHVYSEIGKLKKVLLHRPGKEIENLMPDYLERLLFDDI
                     PFLEDAQKEHDAFAQALRDEGIEVLYLETLAAESLVTPEIREAFIDEYLSEANIRGRA
                     TKKAIRELLMAIEDNQELIEKTMAGVQKSELPEIPASEKGLTDLVESNYPFAIDPMPN
                     LYFTRDPFATIGTGVSLNHMFSETRNRETLYGKYIFTHHPIYGGGKVPMVYDRNETTR
                     IEGGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKQNLGFKKVLAFEFANNRKFMHL
                     DTVFTMVDYDKFTIHPEIEGDLRVYSVTYDNEELHIVEEKGDLAELLAANLGVEKVDL
                     IRCGGDNLVAAGREQWNDGSNTLTIAPGVVVVYNRNTITNAILESKGLKLIKIHGSEL
                     VRGRGGPRCMSMPFEREDI"
     gene            1563..1991
                     /locus_tag="VC394_000002"
     CDS             1563..1991
                     /locus_tag="VC394_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP5993508.1"
                     /translation="MTDKFDANDETRTVYAVVYDNDQPVSTGQFLAETKIEARLTRIV
                     TLADYCGCGYGAKVTEALETYTRREGFYQLTIHSELTAQTFYENLGYQSYGPKCLEDG
                     EYCQSLAKTILKWEKNMDIAMLIAIVGGLLGCYLYLTKNN"
     gene            2031..3044
                     /gene="argF"
                     /locus_tag="VC394_000003"
     CDS             2031..3044
                     /gene="argF"
                     /locus_tag="VC394_000003"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEP5993509.1"
                     /translation="MTQVFQGRSFLAEKDFTRAELEYLIDFSAHLKDLKKRGVPHHYL
                     EGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRMF
                     DGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVKENFGKLEGLTLVY
                     CGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEEEIVTLAEGYAKESGARILITEDA
                     DEAVKGADVLYTDVWVSMGEEDKFKERVELLQPYQVNMNLVQKAGNDKLIFLHCLPAF
                     HDTNTVYGKDVAEKFGVKEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLFI
                     PKV"
     gene            3214..4707
                     /locus_tag="VC394_000004"
     CDS             3214..4707
                     /locus_tag="VC394_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEP5993510.1"
                     /translation="MTEEKKRGFRIPSSYTVLFIIIAIMAVLTWFIPAGAYETAKGGG
                     VISGTYKTVASNPQGFFDILMAPVRGMLGVEGTDGAIQVSFFILMVGGFLGVVNKTGA
                     LDTGIASVVRKNKGREKMLIAILIPLFALGGTTYGMGEETMAFYPLLIPVMIAVGFDS
                     IVAVAIILIGSQIGCLASTINPFATGVAADAAGVSIADGMIWRVIQWVILVGMSIWFV
                     YNYASKIEEDPSKSLVADKEEEHKELFQLQNSGEDLNKRQRNVLTIFTLTFVIMILSL
                     IPWEDFGIKFFTNINTWLTTMPILGGVIGKTMGAFGTWYFPEITMLFIMMGVLVAIVY
                     RMSEEDFFSSFLTGAGEFLGVAMICAIARGIQVIMNGGMITATILHLGETSLSGLSSQ
                     VFVILAYIFYLPMSFLIPSTSGLAGATMGIMAPLGQFSNVPAHLVITAFQSASGILNM
                     ISPTSAIVMGALALGRVDLGTWWKFIGKFIVMVMLVSVLLLVVATFF"
     gene            4724..6055
                     /locus_tag="VC394_000005"
     CDS             4724..6055
                     /locus_tag="VC394_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEP5993511.1"
                     /translation="MESYITPKHQEACVAAIKQIVSYPSVCHEGENGTPFGQAIQDVL
                     EATLDLCQGLGFHTYIDPEGYYGYAELGDQKEVLAILCHLDVVPEGDRQLWKTDPFDC
                     VEADGCLFGRGTQDDKGPTMMALFATKALIDAGVTFNKRIRFIFGTDEETLWRCMNRY
                     NDVEEQATFGFAPDSSFPLTYAEKGLLQAKLVGKGSDKLSLEIGQAYNVVPARASYKG
                     NKLEALQKELDKLGFEYVVKEGELTVYGLAQHTKDAPDGINAMIRLAKALVVLEPNKT
                     LDFLANVIDEDGRALNIFGRIEDEPSGKLSFNAAGLTLTKDKAEIRLDIRIPVLADKE
                     KLVQQLSQKAQEYGLTYEEFDYLAPLYVPLDSELVTTLLSVYRKKTGDQSPAQSSGGA
                     TFARTMKNCVAFGALFPDAVQTEHQENEHIVLADAYRAMDIYAEAIYRLTR"
     gene            6075..7025
                     /gene="arcC"
                     /locus_tag="VC394_000006"
     CDS             6075..7025
                     /gene="arcC"
                     /locus_tag="VC394_000006"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEP5993512.1"
                     /translation="MTKQKIVVALGGNAILSTDASAKAQQEALISTSKSLVKLIKEGH
                     EVIVTHGNGPQVGNLLLQQAAADSEKNPAMPLDTCVAMTEGSIGFWLVNALDNELQAQ
                     GIQKEVAAVVTQVIVDAKDPAFENPTKPIGPFLTEEDAKKQMAESGASFKEDAGRGWR
                     KVVPSPKPVGIKEANVIRSLVDSGVVVVSAGGGGVPVVEDATSKSLTGVEAVIDKDFA
                     SQTLSELVDADLFIVLTGVDNVYINFNKPDQAKLEEVTVSQMKEYITQDQFAPGSMLP
                     KVEAAIAFVENKPNAKAIITSLENIDNVLSANAGTQIIAG"
     gene            7338..8330
                     /locus_tag="VC394_000007"
     CDS             7338..8330
                     /locus_tag="VC394_000007"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEP5993513.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLIAKLDVVEVQGPILSRVGDGM
                     QDNLSGTENPVSVNVLKIPNATFEVVHSLAKWKRHTLARFGFNEGEGLVVNMKALRPD
                     EDSLDQTHSVYVDQWDWEKVIPDGKRNLAYLKETVETIYKVIRLTELAVEARYDIEAV
                     LPKKITFIHTEELVAKYPDLTPKERENAITKEFGAVFLIGIGGVLPDGKPHDGRAPDY
                     DDWTTETENGYHGLNGDILVWNDQLGSAFELSSMGIRVDEEALKRQVEMTGDQDRLAF
                     DWHKSLLNGLFPLTIGGGIGQSRMVMFLLRKKHIGEVQTSVWPQEVRDSYDNIL"
     gene            8453..8992
                     /gene="rsmD"
                     /locus_tag="VC394_000008"
     CDS             8453..8992
                     /gene="rsmD"
                     /locus_tag="VC394_000008"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEP5993514.1"
                     /translation="MRVVSGEFGGRPLKTLDGKITRPTSDKVRGAIFNMIGPYFNGGR
                     VLDLFAGSGGLAIEAVSRGMSAAVLVEKNRKAQAIIQDNIIMTKAENRFTLLKMEAER
                     AIDCLTGRFDLVFLDPPYAKETIVATIEALAAKNLLSEQVMVVCETDKTVLLPKEIAT
                     LGIWKEKIYGISKVTVYVN"
     gene            8982..9473
                     /gene="coaD"
                     /locus_tag="VC394_000009"
     CDS             8982..9473
                     /gene="coaD"
                     /locus_tag="VC394_000009"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020905327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEP5993515.1"
                     /translation="MLTKIGLYTGSFDPVTNGHLDIVKRASGLFDQIYVGIFDNPTKK
                     SYFKLEVRKAMLTQALADFTNVIVVTSHERLAIDVAKELRVTHLIRGLRNATDFEYEE
                     NLEYFNHLLAPNIETVYLISRNKWQALSSSRVRELIHFQSSLEGLVPQSVIAQVEKMN
                     EKT"
     gene            9460..10497
                     /locus_tag="VC394_000010"
     CDS             9460..10497
                     /locus_tag="VC394_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEP5993516.1"
                     /translation="MKRLKKIKWWLVGLLALISLLLALFFPLPYYIEMPGGAYDIRTV
                     LQVNGKEDKRKGAYQFVAVGISRASLAQLLYAWLTPFTEISTAEDTTGGYSDTDFLRI
                     NQFYMETSQNAAIYQALSLAGKPVTLDYKGVYVLDVNNESTFKGTLHLADTVTGVNGK
                     QFTSSAELIDYVSHLKLGDEVTVQFTSDNKPKKGVGRIIKLKNGKNGIGIALTDHTSV
                     NSEDTVIFSTKGVGGPSAGLMFTLDIYDQITKEDLRKGRTIAGTGTIGKDGEVGDIGG
                     AGLKVVAAAEAGADIFFVPNNPVDKEIKKVNPNAISNYEEAKRAAKRLKTKMKIVPVT
                     TVQEALVYLRK"
     gene            10912..11889
                     /locus_tag="VC394_000011"
     CDS             10912..11889
                     /locus_tag="VC394_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEP5993517.1"
                     /translation="MVTIKQVAEEAGVSRSTVSRYISQKGYVSDDARHKIKAAIAKLH
                     YTPNVLAQSLKTKKNQLVGLLLPDISNPFFPRLARGAEEYLKEKGYRVMLGNISDSEA
                     LEEEYVHVLLQSNAAGIITTHDFTKRYPTLAIPVVVVDCVDQETQYGVFSDNRAGGLL
                     AAQTVWQAGAKEVLLIRGPLDNAENINERFEASFSYLQKQDVTMYVCDSQNFDFESIQ
                     LEASYNLKCYPTIDSIIAPSDIHAIAYIHELHSQGKKIPQDVQIIGYDDILMSQFIYP
                     SLSTIHQSSYLMGRYAAELVYTIASQLTVKANRIKLPVHYVERETIRKR"
     gene            12313..12957
                     /locus_tag="VC394_000012"
     CDS             12313..12957
                     /locus_tag="VC394_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1027 domain-containing protein"
                     /protein_id="HEP5993518.1"
                     /translation="MDFSERCQMKKEISPEMYNYNKFPGPKFIHFEEQVKAEGIDLLL
                     LEDVKNAFDTTSFGQRYTEVLLKYDYIVGDWGNEQLRLKGFYKDSDDIKKTNRISRLE
                     DYIKEFCNFGCAYFVLENLHPQDIKFEEERQPRRKKSPKSKSNRRKPNYSNQQPATPK
                     SKSKRVSKEKQPENQAFTSQKRRSNTKHKEKSKRNQTSQLNTKISHFIIRKKDK"
     gene            12961..14040
                     /gene="rlmN"
                     /locus_tag="VC394_000013"
     CDS             12961..14040
                     /gene="rlmN"
                     /locus_tag="VC394_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEP5993519.1"
                     /translation="MKPSIYSLTRDELIAWAVERGQKQFRATQIWDWLYKKRVQSFEE
                     MTNISKDFVSILNDSFCVNPLKQRVVQESADGTVKYLFELPDGMLIETVLMRQHYGHS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNSGEITAQIMLVQKYFDDRKQGERVSHVVVM
                     GIGEPFDNYKNVMCFLRVINDDNGLAIGARHITVSTSGLAHKIRDFANEGVQVNLAVS
                     LHAPNNDLRSSIMRVNRSFPLEKLFSAIEYYIEKTNRRVTFEYIMLNEVNDSIKQAQE
                     LADLTKTIRKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDVLKKNGVNCVVRQEHGT
                     DIDAACGQLRSKTMKKDREKVTATK"
     gene            complement(14122..14748)
                     /locus_tag="VC394_000014"
     CDS             complement(14122..14748)
                     /locus_tag="VC394_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEP5993520.1"
                     /translation="MIAFFSICSGILLTHLFYCLLLYFPDNGQEQLSKQTVIVCDQLT
                     FAKRVFRQLQNHFITSQKIFFSLEISLIIILWASMVHWVSASYCYLLLFSLLFSLFDW
                     RSQEYPFILWLFSFVSLLLFYSINYLSLILLLLGLLAHLRPFSIGAGDFFYLASLALV
                     LDLTSLIWLIQLASLAGITACLLLGIKRIPFIPYLSFGLFWIVLLEHF"
     gene            14976..15503
                     /locus_tag="VC394_000015"
     CDS             14976..15503
                     /locus_tag="VC394_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012997888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEP5993521.1"
                     /translation="MTNTLVENIYASVTHNISKKEASKNEKTKAVLNQAVADLSVAAS
                     IVHQVHWYMRGPGFLYLHPKMDELLDSLNANLDEMSERLITIGGAPYSTLAEFSKHSK
                     LDEAKGTYDKTVAQHLARLVEVYLYLSSLYQVGLDITDEEGDAGTNDLFTAAKTEAEK
                     TIWMLQAERGQGPAL"
     gene            15645..15848
                     /locus_tag="VC394_000016"
     CDS             15645..15848
                     /locus_tag="VC394_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEP5993522.1"
                     /translation="MKTLYDVQQLLKNFGIFVYLGKRLYDIEMMKIELQRLYDSGLLD
                     KRDYLNAELILRREHRLELEKEG"
     gene            15853..16824
                     /locus_tag="VC394_000017"
     CDS             15853..16824
                     /locus_tag="VC394_000017"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEP5993523.1"
                     /translation="MSQKLLGIDLGGTTIKFGILTAAGEVQEKWAIETNILEGGKHIV
                     PDIIASIKHRLDLYGLSSADFVGIGMGSPGAVDRDTNTVTGAFNLNWKETQEVGSVVE
                     KELGIPFAIDNDANVAALGERWVGAGENNPDVVFMTLGTGVGGGIIADGNLIHGVAGA
                     GGEIGHMIVEPENGFACTCGSHGCLETVASATGVVKVARLLAEAYEGDSAIKAAIDNG
                     EGVTSKDIFMAAEAGDSFADSVVEKVGYYLGLASANISNILNPDSVVIGGGVSAAGEF
                     LRSRIEKYFVTFTFPQVRYSTKIKIAELGNDAGIIGAASLARQFITK"
     gene            16834..17223
                     /locus_tag="VC394_000018"
     CDS             16834..17223
                     /locus_tag="VC394_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEP5993524.1"
                     /translation="MSPITLILWLLLVGIVGYYTWNYFSFRKMAKQVDNETFKDVMRQ
                     GQLIDLREPAAFRAKHILGARNFPAQQFDAAIKGLRKDKPVLIYENMRPQYRVPAVKK
                     LKKAGFEDVYVLKDGIDYWDGKVKQTT"
     gene            17406..19247
                     /gene="typA"
                     /locus_tag="VC394_000019"
     CDS             17406..19247
                     /gene="typA"
                     /locus_tag="VC394_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEP5993525.1"
                     /translation="MTNLRNDIRNVAIIAHVDHGKTTLVDELLKQSHTLDERKELQER
                     AMDSNDLEKERGITILAKNTAVAYNDVRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLIPIVVVNKIDKPSARPAEVVDEVLELFIELGADDEQ
                     LEFPVVYASAINGTSSLSDDPADQEHTMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDCVEALPILRIDEPTLQMTFLVNNSPFAGREGKWITSR
                     KVEERLLAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IIKEIDGVKCEPFERVQIDTPEEYQGAIIQSLSERKGDMLDMQMVGNGQTRLIFLIPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPVVQGEIGGRHRGALVSIENGKATTYSIMR
                     IEERGTIFVNPGTEVYEGMIVGENSRDNDLGVNITTAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAARDKANKKKKSAE"
     gene            19369..19620
                     /locus_tag="VC394_000020"
     CDS             19369..19620
                     /locus_tag="VC394_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEP5993526.1"
                     /translation="MFYLIMAVSIISYYLYMAPKSVRNTLGMIGLVGLVALLIVLAGL
                     SFIKIMQTPPEFFIGMGMVALGYFALKDVRKMTKKPRVK"
     gene            19976..21334
                     /locus_tag="VC394_000021"
     CDS             19976..21334
                     /locus_tag="VC394_000021"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEP5993527.1"
                     /translation="MKVISNFQNKKILILGLAKSGEAAAKLLTKLGALVTVNDSKPFD
                     QNPAAQALLEEGIKVICGSHPVELLDEDFEYMVKNPGIPYDNPMVKRALAKEIPILTE
                     VELAYFVSEAPIIGITGSNGKTTTTTMIADVLNAGGQSALLSGNIGYPASKVVQKAIA
                     GDTLVMELSSFQLVGVNAFRPHIAVITNLMPTHLDYHGSFEDYVAAKWMIQAQMTESD
                     YLILNANQEISATLAKTTKATVIPFSTQKVVDGAYLKDGILYFKEQAIIAATDLGVPG
                     SHNIENALATIAVAKLSGIADDIIAQCLSHFGGVKHRLQRVGQIKDITFYNDSKSTNI
                     LATQKALSGFDNSRLILIAGGLDRGNEFDDLVPDLLGLKQMIILGESAERMKRAANKA
                     EVSYLEARNVAEATELAFKLAQTGDTILLSPANASWDMYPNFEVRGDEFLATFDCLRG
                     DA"
     gene            21334..22416
                     /locus_tag="VC394_000022"
     CDS             21334..22416
                     /locus_tag="VC394_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEP5993528.1"
                     /translation="MPKKILFTGGGTVGHVTLNLILIPKFIKDGWEVHYIGDKNGIEH
                     TEIEKSGLDVTFHAIATGKLRRYFSWQNLADVFKVALGLLQSLFIVAKLRPQALFSKG
                     GFVSVPPVVAAKLLGKPVFIHESDRSMGLANKIAYKFATTMYTTFEQEDQLSKVKHLG
                     AVTKVFKDANQMPESTQLEAVKEYFSRDLKTLLFIGGSAGAHVFNQFISDHPELKQRY
                     NIINITGDPHLNELSSHLYRVDYVTDLYQPLMAMADLVVTRGGSNTLFELLAMAKLHL
                     IVPLGKEASRGDQLENATYFEKRGYAKQLQEPDLTLHNFDQAMADLFEHQADYEATML
                     ATKEIQSPDFFYDLLRADISSAIKEK"
     gene            22417..23565
                     /locus_tag="VC394_000023"
     CDS             22417..23565
                     /locus_tag="VC394_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEP5993529.1"
                     /translation="MAKDKEKQSDDKLVLTEWQKRNIEFLKKKKQQAEEEKKLKEKLL
                     SDKKAQQQAQNASEAVELKTDEKTDSQEIESETTSKPKKPKKVRQPKEKSATQIAFQK
                     SLPVLLGALLLMAVSIFMITPYSKKKEFSVRGNHQTNLDELIKASKVKASDYWLTLLT
                     SPGQYERPILRTIPWVKSVHLSYQFPNHFLFNVIEFEIIAYAQVENGFQPILENGKRV
                     DKVRASELPKSFLILNLKDEKAIQQLVKQLTTLPKKLVKNIKSVSLANSKTTADLLLI
                     EMYDGNVVRVPQSQLTLKLPYYQKLKKNLENDSIVDMEVGIYTTTQEIENQPEVPLTP
                     EQNAADKEGDKPGEHQEQTDNDSETPANQSSPQQAPPSPETVLEQAHG"
     gene            23777..25141
                     /gene="ftsA"
                     /locus_tag="VC394_000024"
     CDS             23777..25141
                     /gene="ftsA"
                     /locus_tag="VC394_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEP5993530.1"
                     /translation="MARNGFFTGLDIGTSSIKVLVAEFISGEMNVIGVSNVPSTGVKD
                     GIIIDIEAAATAIKTAVEQAEEKAGMTIEKVNVGLPANLLQIEPTQGMIPVPSESKEI
                     KDEDVDSVVKSALTKSITPEREVISLVPEEFIVDGFQGIRDPRGMMGIRLEMRGLIYT
                     GPSTILHNLRKTVERAGIKVENIIISPLAMAKTILNEGEREFGATVIDMGGGQTTVAS
                     MRAQELQYTNIYAEGGEYITKDISKVLKTSLAIAEALKFNFGQAEISEASITETVKVD
                     VVGSEEPVEVTERYLSEIISARIRHILDRVKQDLERGRLLDLPGGIVLIGGGAIMPGV
                     VEIAQEIFGVTVKLHVPNQVGIRNPMFSNVISLVEYVGMMSEVDVLAQTAVSGEELLR
                     RKPIDFSGQESYLPDYDDSRRPESTIGYEQQASQTAYDSQVPSDPKQKISERVRGIFG
                     SMFD"
     gene            25165..26484
                     /gene="ftsZ"
                     /locus_tag="VC394_000025"
     CDS             25165..26484
                     /gene="ftsZ"
                     /locus_tag="VC394_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEP5993531.1"
                     /translation="MAFSFDTASIQGAIIKVIGVGGGGGNAINRMIDEGVAGVEFIAA
                     NTDIQALSSSKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEEILTEALTGADMVFI
                     TAGMGGGSGTGAAPVIARIAKSLGALTVAVVTRPFGFEGNKRGNFAIEGIEELREQVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITSPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERIVEAARKAIYSPLLETTIDGAQDVIVNVTGGLDMTLTEA
                     EEASEIVGQAAGQGVNIWLGTSIDDTMKDDIRVTVVATGVRQEKAEQVSGFRQPRTFT
                     QTNAQQVAGAQYASDQAKQSVQPGFDRRSNFDFDMGESREIPSAQKVISNHNQNQGSA
                     FGNWDLRRDNISRPTEGELDNHLNMSTFSANDDSDDELETPPFFKNR"
     gene            26487..27158
                     /locus_tag="VC394_000026"
     CDS             26487..27158
                     /locus_tag="VC394_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEP5993532.1"
                     /translation="MDLLTNKKKIFETIRLSTEAANRTNDSVSVIAVTKYVDSTIAGQ
                     LIEAGIEHIAENRVDKFLEKYDALKYMPVKWHLIGTLQRRKVKEVINYVDYFHALDSV
                     RLALEINKRADHPVKCFLQVNISKEESKHGFNISEIDEAIEEIGKMEKIQLVGLMTMA
                     PANASKESIITIFRQANQLRKNLQLKKRKNMPFTELSMGMSNDYPIAIQEGSTFIRIG
                     RAFFH"
     gene            27170..27826
                     /locus_tag="VC394_000027"
     CDS             27170..27826
                     /locus_tag="VC394_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEP5993533.1"
                     /translation="MAFKDTFNKMISYFDTDEVNEVEEDVAASTDNVIPRSQQSVRAS
                     SHPKQEPRNNHVQQDHQARSQEQTRSQMYPKHGTSERYYQQSQPKEGHEMVDRRKRMS
                     TSGIANRREQYQQSTCSDQTTIALKYPRKYEDAQEIVDLLIVNECVLIDFQFMLDAQA
                     RRCLDFIDGASKVLYGSLQKVGSSMYLLAPSNVSVNIEEMTIPHTTQDIGFDFDMKRR
                     "
     gene            27831..28085
                     /locus_tag="VC394_000028"
     CDS             27831..28085
                     /locus_tag="VC394_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEP5993534.1"
                     /translation="MILILSILLRLIKVYTYLLIAYALMSWFPGAYDSKIGRLISGIV
                     EPILKPFRAFNLQFAGLDFTIFVVIISLNFLAQVLVRVFI"
     gene            28085..28876
                     /locus_tag="VC394_000029"
     CDS             28085..28876
                     /locus_tag="VC394_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEP5993535.1"
                     /translation="MVSHSKIYQHFHQEEYPFIDRMSDMINRVGDYYLLEVTEFLNPR
                     EVMILKSLIALTDLKMFVSTDYYPSEYGRVIIAPGYYDLEQSDFQIALVEISYQAKFN
                     QLTHSQILGTLINELGVKRNLFGDVFVEMGYAQLMIKRELLDYFLGTITKIAKTSVKL
                     REVNFDQLIRSIDNSQTLDILVSSFRLDGVVATILKKSRTQVIALIEANKIKVNYRLA
                     NKASDNLVIGDMVSIRGHGRFTLLADNGVTKHGKQKITLSKMIHK"
     gene            28886..29644
                     /locus_tag="VC394_000030"
     CDS             28886..29644
                     /locus_tag="VC394_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEP5993536.1"
                     /translation="MALTTLEIKDKTFKTKFRGYCEEEVNEFLDIVVDDYEALVRKNR
                     DNEARIKDLEEKLSYFDEMKESLSQSVILAQETAEKVKATANAEATNLVSKATYDAQH
                     LLDESKAKANQMLRDATDEAKRVAIETEELKRQTRVFHQRLISSIESQLSLSNSPEWD
                     ELLQPTAIYLQNSDDAFKEVVKTVLNEDIPESDDSASFDATRQFTPEELEELQRRVDE
                     SNKELEAYQLDSQSDSTTEPEVNLSETQTFKLNI"
     gene            29917..32718
                     /locus_tag="VC394_000031"
     CDS             29917..32718
                     /locus_tag="VC394_000031"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEP5993537.1"
                     /translation="MKLKETLNLGKTAFPMRAGLPNKEPQWQAAWEQAELYKKRQELN
                     AGKPAFHLHDGPPYANGNIHVGHALNKISKDIIVRSKSMSGFQAPYVPGWDTHGLPIE
                     QVLAKQGIKRKEMDLAEYLEMCRQYALSQVDKQRDDFKRLGVSADWENPYVTLDPQFE
                     ADQIRVFGAMAEKGYIYRGAKPVYWSWSSESALAEAEIEYHDIDSTSLYYANKVKDGK
                     GILDTNTYIVVWTTTPFTVTASRGLTVGPDMDYLVVKPAGSDRQYVVAEGLLDSLAGK
                     FGWESFETLASHKGADLEYIVTEHPWDTDVEELVILGDHVTLESGTGIVHTAPGFGED
                     DYNVGTKYKLEVAVTVDERGLMTENAGPDFHGQFYNKVTPIVIDKLGDLLLAQEVINH
                     SYPFDWRTKKPIIWRAVPQWFASVSDFRQDILDEIEKTTFHPSWGETRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTKEVTDHVADLFQENGSIIWWQKEAKDLLPEGF
                     THPGSPNGEFTKETDIMDVWFDSGSSWNGVMNTKENLSYPADLYLEGSDQYRGWFNSS
                     LITSVAVNGHAPYKAILSQGFVLDGKGEKMSKSKGNIISPNDVAKQYGADILRLWVAS
                     VDTDNDVRVSMEILGQVSETYRKIRNTLRFLIANTSDFNPATDTVAYADLGAVDKYMT
                     IVFNQLVATITDAYERYDFMAIYKAVVNFVTVDLSAFYLDFAKDVVYIEAANSLERRR
                     MQTVFYDILVKITKLLTPILPHTTEEIWSYLEHESEAFVQLAEMPVAETFSAQEDILE
                     AWSAFMTLRTQAQKALEEARNAKIIGKSLEAHLTIYASEEVKTLLTALDSDIALLLIV
                     SQLTIADLADAPADAVAFEGIAFMVEHAIGEVCERSRRIDPTTRMRSYNAFVCDHSAK
                     IIEENFPEAVAEGFEESDK"
     gene            complement(32983..33285)
                     /locus_tag="VC394_000032"
     CDS             complement(32983..33285)
                     /locus_tag="VC394_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEP5993538.1"
                     /translation="MRLINTTSSHPELIRNQLKNTDAYLVEVYSAGNTDVIFTQAPKH
                     YELLISNKYRAIKEDELDIIREFFLKRKIDPKIVIPGQSKTLHTNNLIEISFQTSV"
     gene            complement(33336..33791)
                     /locus_tag="VC394_000033"
     CDS             complement(33336..33791)
                     /locus_tag="VC394_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEP5993539.1"
                     /translation="MMIPTFGHKNAHKDYVTRYGVYAIIPNHEQTKIILVQAPNGSWF
                     LPGGEIEAGEGQLQALERELIEELGFSATIGSYYGQADEYFYSRHRDTHFYHPAYLYE
                     VTAFQAVSKPLEDFNNLSWFSPIEAIAKLKRESHQWGVKEWQKKHHSTN"
     gene            complement(33919..36201)
                     /locus_tag="VC394_000034"
     CDS             complement(33919..36201)
                     /locus_tag="VC394_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEP5993540.1"
                     /translation="MLCQNCNLNESTIHLYTSVNGKQRQVDLCQNCYQIMKSDPANSI
                     LNGLTPGYRAQDRSTSPFFDDFFGDLNNFRAFGNLPNTPPTQAGQNGNGGGRYGGNYN
                     GQRPAQPQTPNQQAKGLLEEFGINVTDIARNGNIDPVIGRDEEITRVIEILNRRTKNN
                     PVLIGEPGVGKTAVVEGLAQKIIDGTVPQKLQGKQVIRLDVVSLVQGTGIRGQFEERM
                     QKLMEEIRNRKDVILFIDEIHEIVGAGSAGDGNMDAGNILKPALARGELQLVGATTLN
                     EYRIIEKDAALERRMQPVKVDEPSVEETITILKGIQPKYEDYHHVKYSPAAIEAAAHL
                     SNRYIQDRFLPDKAIDLLDEAGSKMNLTLNFVDPKEIDKRLIEAENLKAQATRDEDYE
                     RAAYFRDQITKYKEMQAQKVDEQDIPIITEKTIEAIVEQKTNIPVGDLKEKEQSQLVN
                     LANDLKAHVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLA
                     IELFGSTNNMIRFDMSEYMEKHAVAKLVGAPPGYVGYEEAGQLTEQVRRNPYSLILLD
                     EVEKAHPDVMHMFLQVLDDGRLTDGQGRTVSFKDTIIIMTSNAGTGKSEASVGFGAAR
                     EGRTSSVLGELSNFFSPEFMNRFDGIIEFKALSKEHLLHIVDLMLEDVNERLGYNGIH
                     LDVTQKVKEKLVDLGYDPKMGARPLRRTIQDYIEDAITDYYLEHPTEKQLRALMTNSE
                     NITIKAVKEGDSFLNEESLD"
     gene            36499..36729
                     /locus_tag="VC394_000035"
     CDS             36499..36729
                     /locus_tag="VC394_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003105222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEP5993541.1"
                     /translation="MESHLVRIINRLELMVTDGGNLKRNFERDGVVVAEVAFSNDPEN
                     GPVFTLRDVEARESYSFDSIDLIAMEIYDLLY"
     gene            36856..37542
                     /locus_tag="VC394_000036"
     CDS             36856..37542
                     /locus_tag="VC394_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEP5993542.1"
                     /translation="MDLSFLPKYWAYFNYGVLVTIMISVSVVFFGTLIGVLVTLIKRS
                     HVKPLTWVVNLYVWIFRGTPMVVQIMIAFAWMHFNNMPTIGFGVLDLDFSRLLPGIII
                     ISLNSGAYISEIVRAGIEAVPKGQLEAAYSLGIRPQNAMRYVILPQAFKNILPALGNE
                     FITIIKDSAFLQTIGVMELWNGAQSVVTATYSPISPLLVAAFYYLMVTTVMAQLLAVL
                     ERHMAQGGNH"
     gene            37542..38276
                     /locus_tag="VC394_000037"
     CDS             37542..38276
                     /locus_tag="VC394_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEP5993543.1"
                     /translation="MTEAIISIKDLHKYYGHNEVLKGIDLDIMPGEVVVIIGPSGSGK
                     STLLRTMNLLEVPTKGQIRFEGIDITDKKNDIFSMREKMGMVFQQFNLFPNMTVLENI
                     TLSPIKTKGMAKAEADKTALSLLDKVGLSEKAKAYPASLSGGQQQRIAIARGLAMDPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAKEVADRVIFMDGGVI
                     VEEGSPNQLFDLTKEERTKDFLSRVL"
     gene            38425..39834
                     /locus_tag="VC394_000038"
     CDS             38425..39834
                     /locus_tag="VC394_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEP5993544.1"
                     /translation="MVSVMWFRRDLRIDDQKALAHAIASQSPTLCVFQFNKEQLNLLH
                     SRNQSSFITSVLAFRKLLKCESIDLYLMYGDLMTCFEQLLTQLKDWTDVYFNYDESGY
                     GRLRDQKAAQFFKKNGIAVHTYQDHYLHGSQEIINQSGQPYKVFTPYYRIWQNYPKET
                     PIKVELSQGRWLNLETPDDVLRTVESFKDEKYQDVATFDEASKQLNRFIQDQLAAYHA
                     NRDFPAQLGTSRLSPFLRIGAIGIRTVYHAVRQAPNSLGQATFLKELAWRDFYNMVYV
                     AYPDQKTQPIQKAFSQIEWVNNPDWFQLWKEGKTGYPIVDAAMLQLQKTGWMHNRLRM
                     IVASFLTKDLLCDWRLGEQYFQQQLIDYDAASNIGGWQWAASTGTDAVPYFRIFNPVT
                     QGKRFDPKGEFIKAYLPQLEHVPEKYLHEPWKMPKYLQESVSCIIGTDYPQPIVDHAK
                     QREQAIAKYEWAKEKAKIE"
     gene            40012..41706
                     /locus_tag="VC394_000039"
     CDS             40012..41706
                     /locus_tag="VC394_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEP5993545.1"
                     /translation="MTYNEVYQEWLHNNDLSDDIKADLAAIKDNEAEIQDRFYKTLEF
                     GTAGLRGKLGAGTNRMNTYMVGKAAQALANTIIDHGPEAVKKGIAVSYDVRYQSRTFA
                     ELTCSIMAANGIKAYLYKGIRPTPMCSYAIRALGCISGVMITASHNPQAYNGYKAYWQ
                     EGSQILDDIADQIAQHMAALTQYEEIKQMPFEKALDSGLVTYIDESIEEAYKKEVLGL
                     TINDTDIDKSVRVVYTPLNGVGNLPVREVLRRRGFENVYVVPEQEMPDPDFTTVGYPN
                     PEVPKTFAYSEKLGKAVDADILIATDPDCDRVALEVKNAVGDYVFLNGNKIGALLSYY
                     IFSQRFDLGNLPANPVLVKSIVTGDLSRAIASHYGIETVETLTGFKNICGKANEYDVT
                     KQKNYLFGYEESIGFCYGTFVRDKDAVSASMMIVEMAAYYKKKGQNLLDVLQTIYATF
                     GYYNERQIALELEGIEGQKRIARIMEDFRQTPIASVAEMALDKTIDFIDGYQDFPKQN
                     CLKFYLDDGSWYALRPSGTEPKIKFYLYTIGQTQENSATKLDAIEAACRTKINQVN"
     gene            41914..42768
                     /locus_tag="VC394_000040"
     CDS             41914..42768
                     /locus_tag="VC394_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEP5993546.1"
                     /translation="MTELIDGKALAQKMQQELAAKVNNLKQKKGIVPGLAVILVGDDP
                     ASQVYVRNKERAALTVGFKSETVRLSEFICQEELIAVIERYNADNTIHGILVQLPLPN
                     HINDKKIILAIDPKKDVDGFHPMNTGHLWSGRPLMVPCTPSGIMELLREYNVNLEGKH
                     AVIIGRSNIVGKPMAQLLLDKNATVTLTHSRTRQLEEVCRCADVLIVAIGQGHFITKQ
                     YIKDGAIVIDVGMNRDDNGKLIGDVAFDEVAEVAAKITPVPGGVGPMTIAMLLEQTYQ
                     SALRSTHK"
     gene            42921..44261
                     /locus_tag="VC394_000041"
     CDS             42921..44261
                     /locus_tag="VC394_000041"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEP5993547.1"
                     /translation="MADYLTVTHLTKYLKLKFDRDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDESAVIQATMWAGVYKKLGFDLEEGMKINVIGRVQLYEPSGSYSIVIEKAEPDG
                     IGALALQFEQLKKKLTAEGYFEQKHKQPLPQFVSKIGVITSPSGAVIRDIITTVSRRF
                     PGVEILLFPTKVQGDGAAQEVVANIRRANQREDLDLLIVGRGGGSIEDLWAFNEEIVV
                     QAIFESQLPVISSVGHETDTTLADFVADRRAATPTAAAELATPITKTDLMSWIVERQN
                     RSYQACLRRIKQRQEWVDKLSQSVIFRQPERLYDAYLQKIDRLSMTLMNTMKDRLSSA
                     KENKVQLDHSLANSQLQTKIERYQDRVATAKRLLMANMASQYDSQLARFEKAQDALLS
                     LDASRIIARGYAMIEKNQALVASVSQITKGDQLTIKMRDGQLDVEVKDVKNENI"
     gene            44239..44454
                     /locus_tag="VC394_000042"
     CDS             44239..44454
                     /locus_tag="VC394_000042"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEP5993548.1"
                     /translation="MSKTKTFEENLQDLETIVNKLENGDVPLEEAISEFQKGMLLSKE
                     LQKTLQAAEKTLVKVMQADGTEVDMDD"
     gene            44454..45326
                     /locus_tag="VC394_000043"
     CDS             44454..45326
                     /locus_tag="VC394_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEP5993549.1"
                     /translation="MDKLARIDEAIRRYYKTTSNGVSEELIDAILYSVDSGGKRIRPL
                     ILLEMIEGFGVSLQNAHFDLAAALEMIHTGSLIHDDLPAMDNDDYRRGRLTNHKQFGE
                     ATAILAGDSLFLDPFGLIAQAELNSEVKVALIQELSLASGTFGMVGGQMLDMKGENQA
                     LSLPQLSLIHLNKTGKLLTFPFKAAALVTEQAITVRQQLEQAGMLIGHAFQIRDDILD
                     VTASFEDLGKTPKKDLFAEKATYPSLLGLEASYQLLTESLDQALTIFQTLESDVGFKP
                     QIITKLIEGLRLNA"
     gene            45319..46146
                     /locus_tag="VC394_000044"
     CDS             45319..46146
                     /locus_tag="VC394_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEP5993550.1"
                     /translation="MPKERVDVLAYKQGLFETREQAKRGVMAGLVVSVINGQRYDKPG
                     DKIDDGTELKLKGEKLKYVSRGGLKLEKGLHVFGVSVANQIGIDIGASTGGFTDVMLQ
                     DGAKLVYAVDVGTNQLVWKLRQDPRVRSMEQYNFRYAQPEDFNEGQPVLASIDVSFIS
                     LSLILPALHNVLSDQGQVIALIKPQFEAGREQIGKKGIVKDKQIHEKVIQKVMDFASG
                     YGFTVKGLDFSPIQGGHGNIEFLAHLAKSQTPETLAPHLIQEVVAKAHKEFEKHEKE"
     gene            46133..46603
                     /locus_tag="VC394_000045"
     CDS             46133..46603
                     /locus_tag="VC394_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEP5993551.1"
                     /translation="MKKSERLELIKKMVLTHPIETQHDLLRLLAEHGLELTQATISRD
                     MNEIGIVKIPSGSGRYIYGLSQDSGKKIVQGPRSIKSTILAVSDKTKGLEQHLYLKVV
                     PGNSKLIKRYLLADFSKAIFSLIADDDSLLLIAKSPSEADMIRQEILLWMQGIT"
     gene            46625..48286
                     /gene="recN"
                     /locus_tag="VC394_000046"
     CDS             46625..48286
                     /gene="recN"
                     /locus_tag="VC394_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEP5993552.1"
                     /translation="MLLEISIKNFAIIDEISLNFENGMTVLTGETGAGKSIIIDAMNM
                     MLGARASTEVIRRGANKAEIEGFFSVDATPELVACLESSGIAMEEELIIRRDIFANGR
                     SVSRINGQMVNLATLKQVGQFLVDIHGQHDQEELMRPQLHQQILDAFGDKAFEQLKEN
                     YQLIFDRYKSLRRQVIDKQKNEKEHKDRIDMLAFQIAEIEAAALSRGEDDRLNQERDR
                     LMNHKQIADTLTNAYVMLDNDDFSSLSNIRSSMNDLLSIEQFDSEYKGMSTSISEAYY
                     ILEEVSKQLSDTIDQLDFDGGRLQEIEFRLDTLNSLTRKYGGNVNDVLDYYDNIIKEY
                     QLLTGDDLSSGDLESELKALEKQLVAAATNLSERRHQLAEQLEAEIKAELKELYMEKA
                     DFKVHFTTSKFNRDGNESLEFYISTNPGEGFKPLVKVASGGELSRLMLAIKAAISRKE
                     DKTSIVFDEVDTGVSGRVAQAIAQKIYKIGRHGQVLAISHLPQVIAIADYQYFISKES
                     KEESTVSKVRLLTPEERVEEIASMIAGTDMTQAALTQARELLAKH"
     gene            48458..49399
                     /locus_tag="VC394_000047"
     CDS             48458..49399
                     /locus_tag="VC394_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEP5993553.1"
                     /translation="MTSKKACLSSIIVLASLTCGNDTVSANHLSATGDKFDDCSTLVE
                     KDVAPKDELEMLAWSSSQTTDDADRDYEDFLDDDSFISQNETDKMFENLTDDRLLDEL
                     DELDELDELDEEDTIEPEQNVIMPSDDELFDLTDAVETRLTVSSAPHLEAELPKPHLR
                     SLSDTALRSGEIRGHLDNKLDALSVTATKLALTMAQKFDLTTHVYSIGESFSEVLAAH
                     YEDRKAESAFSKKKRFHLPIATPDVVIEELRRLVSSIGSSKEDVSVPYNRKLGMAVAK
                     RKIALPQTGEEFSYYPALLGLMILGLTPIMIPKKINN"
     gene            49615..50466
                     /locus_tag="VC394_000048"
     CDS             49615..50466
                     /locus_tag="VC394_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEP5993554.1"
                     /translation="MEKYMGTIKIVTDSSITIEPELIKALDITVVPLSVMIDSKLYSD
                     NDLKEEGHFLSLMKASKSLPKTSQPPVGLFAETYENLVKKGVTDIVAIHLSPALSGTI
                     EASRQGAEIAEAPVTVLDSGFTDQAMKFQVVEAAKMAKAGASLNEILAAVQAIKSKTE
                     LYIGVSTLENLVKGGRIGRVTGVLSSLLNVKVVMALKNDELKTLVKGRGNKTFTKWLD
                     SYLAKNSHRPIAEIAISYAGEASLALTLKERIAAYYNHSISVLETGSIIQTHTGEGAF
                     AVMVRYE"
     gene            50459..51301
                     /locus_tag="VC394_000049"
     CDS             50459..51301
                     /locus_tag="VC394_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEP5993555.1"
                     /translation="MNNRHLFSGIFFFVISLCLAFLLLNIIIPKSNSRLKKSDFLTKE
                     QVAIQYVAIGDSLTEGVGDLTHQGGFVPLLTNDLSEYFKANVNHQNYGVSGDTSQQIL
                     DRMIKQKQIQLSLKKADIMTLTVGGNDVMAVIRKNLADLQVSSFRKPARQYQKRLRQI
                     IELARKDNKDLPIFILGIYNPFYLNFPELTDMQKVIDDWNTKTKEVVGEYDRVYFVPI
                     NDLLYKGINGQEGIVHSSGDQTTIVNDALFTGDHFHPNNTGYQIMSNAVMEKIKKHEK
                     KIKP"
     gene            51279..51866
                     /locus_tag="VC394_000050"
     CDS             51279..51866
                     /locus_tag="VC394_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HEP5993556.1"
                     /translation="MKKKSNLNWWKWSFLCLLAFNTAFLMVIASRLIQVREPESELIA
                     KKPVKNIKIGTFVTTREQLNETVASYLKDYQTKKMSYKFYATSSSILFEGTYQLLGYE
                     VPLYIYFQPHRLENGAVQLQVISFSVGTLPLPEKDVLQYLKSSYKLPSFVKVMPNQSA
                     IVVNLQDIQNDAKVYLKAKKIDLFNDEISFNIYKK"
     gene            51965..52240
                     /locus_tag="VC394_000051"
     CDS             51965..52240
                     /locus_tag="VC394_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEP5993557.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVDAVFSTIEAFLAEGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGAEIEIAASKVPAFKAGKALKDAVK"
     gene            52594..54456
                     /gene="cadA"
                     /locus_tag="VC394_000052"
     CDS             52594..54456
                     /gene="cadA"
                     /locus_tag="VC394_000052"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEP5993558.1"
                     /translation="MTIRQWMADHLHLMETLACLVLIIIGLAFLHSFPQVASAIFITA
                     FLIGGYASAKTGILDLVKNKHLSVDILMILAAIGAGIIGYWLEGALLIFIFSLSNTLE
                     EMAMEKSKDAISALMSLTPDTARQYQEDGHILEVETRSLSVGDRLQVRKGEAVPIDGQ
                     LLSPFGQFDESMVTGEPITVDKAEGQDLIGGTINQGQTIDMLVTIENDDTLFAKIINL
                     VESAQEKKSKTATFIESLEDGYVKFVLVLIPAFILFSHFVLSWTWLAAFYRGMILLTV
                     ASPCALIASSTPASLAAISRAARKGLIIKGGDIVDNMGDIKAVVMDKTGTLTQGKPSV
                     VNAHYLEDELLVNRLVKGAETASTHPISKALLEYTEKLEPLTFDHLEEISGKGFQGFY
                     QGQEWRIGKKTFILEKVQDLSAFEETIQVEENQGKTLIFVSRDHQLIAYYALLDDIKI
                     ESKRAIKSLHAMGIKTVMLTGDQERTANYVAQKLGIDEVVANCMPQDKVAKLAELKTK
                     YGFVAMVGDGINDAPALAQADVSYAIGSGTDIAMESADSVIMDDLTRIPFSIQLSRKM
                     KTIIKQNIVFALSVITLLILANVFQVVNLPLGVVGHEGSTILVILNGLRLLSFK"
     gene            complement(54756..55691)
                     /locus_tag="VC394_000053"
     CDS             complement(54756..55691)
                     /locus_tag="VC394_000053"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004228450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEP5993559.1"
                     /translation="MVSTATQIGHFSFDNCLMNAAGVYCMTKEELMEVEKSQAASFVT
                     KTGTLEVRPGNPEPRYADTRLGSINSMGLPNNGFRYYLDFVSDLAKTGQHKPHFLSVV
                     GLSPTETETILKAIMASDYEGLVELNLSCPNVPGKPQIAYDFETTDQLLENIFTYYTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLSFVNCVNSIGNGLVIEDEQVLIKPKNGFGGI
                     GGDYIKPTALANVHAFYKRLKPSIHIIGTGGVKTGRDAFEHILCGASMVQIGTALHQE
                     GPAIFERVTKELKTIMVEKGYQSLDDFRGNLRYKD"
     gene            55930..56625
                     /locus_tag="VC394_000054"
     CDS             55930..56625
                     /locus_tag="VC394_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEP5993560.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIEFDLAFTSVLTRAIKTTNLALENAGQLWVPTEKSWRLNERHYGALTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPAMAKDDEYSAHKDRRYADLDPALIPDAENLKVTLERAM
                     PYWEEKIAPALLDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFELDE
                     KLNIVKEYYLGGE"
     gene            56732..57253
                     /locus_tag="VC394_000055"
     CDS             56732..57253
                     /locus_tag="VC394_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEP5993561.1"
                     /translation="MKAEDRRQKIIECLNSEQKAVSATRLGKLLGVSRQVIVGDIALL
                     RAQQIDIISTPKGYIMSTALYSHQFCARIVCQHNVEETKKELEIILAHQGIITTVEVE
                     HPIYGMITAPLNIKTHSDVTNFMSKLSQSKAELLSSLTEGLHSHLISCPSQEAFLAIK
                     HDLELAGILYKGI"
     gene            57414..57956
                     /locus_tag="VC394_000056"
     CDS             57414..57956
                     /locus_tag="VC394_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5993562.1"
                     /translation="MTKKPTQLIAYTSILVAFAILIPIIMPLKLIIGPASFTLASHVP
                     LFLAIFMSIPVAILVALGTTLGFLLAGLPLIIVLRALSHLLFAILAAWWLSRKPQLMT
                     SAVKCFSFAFFINVIHGLAEFLVVYILTATTATSMSYFWSMLGLIGLGSLIHGILDFY
                     LALVLWRFLAKNLKLPTINA"
     gene            58080..58859
                     /locus_tag="VC394_000057"
     CDS             58080..58859
                     /locus_tag="VC394_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEP5993563.1"
                     /translation="MKTPEQILEATIHIGEHKVTKTFLAKSILGFIGGAMISLGYLLY
                     VRIAASGLETFGAFSSIVGACAFPIGLIIILMAGGELITGNMMAVSAALLAKKIKFSE
                     LAKNWLIITLFNVIGAVFVAFVFGHFLGLTSAGIFKEEVIEVAHAKIAASPLQALVSG
                     IGCNWFVGLALWLCYGANDAAGKFLGTWFPVMTFVALGFQHSVANAFVIPAAIFEGGA
                     TWLDFATNFIFVYSGNIIGGAIFVSFLYFKVYYHPQKSKTQ"
     gene            59657..60253
                     /gene="recR"
                     /locus_tag="VC394_000058"
     CDS             59657..60253
                     /gene="recR"
                     /locus_tag="VC394_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEP5993564.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSNEDVNDFA
                     KNLLAAKRELTYCSICGNLTDDDPCHICTDTSRDQTTILVVEDAKDVSAMEKIQEYHG
                     YYHVLHGLISPMNGVGPDDINLKSLITRLMDGKVSEVIVATNATADGEATSMYISRVL
                     KPAGIKVTRLARGLAVGSDIEYADEVTLLRAIENRTEL"
     gene            60354..61400
                     /locus_tag="VC394_000059"
     CDS             60354..61400
                     /locus_tag="VC394_000059"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEP5993565.1"
                     /translation="MSKQTLVLLYGGRSAEREVSVLSAESVMRAVNYDKFLVKTYFIT
                     QMGQFIKTQQFSEKPSESERLMTNETIELTQKIKPSDIYEEGAVVFPVLHGPMGEDGS
                     IQGFLEVLRMPYIGTNVMSSSIAMDKITTKRVLESIGIPQVAYTVYIDGQDLEACLVE
                     TLARLTFPIFVKPANMGSSVGISKAQTKVELRKAIQLALTYDSRVLIEQGVVAREIEV
                     GLLGNDKVKSTLPGEVIKDVDFYDYQAKYVDNKITMAIPADVDQSIVTEMRSYAEVAF
                     KALGGCGLSRCDFFLTQDGQVYLNELNTMPGFTQWSMYPLLWENMGLAYPDLIEELVT
                     LAQEMFDQRESHLI"
     gene            61599..62990
                     /locus_tag="VC394_000060"
     CDS             61599..62990
                     /locus_tag="VC394_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEP5993566.1"
                     /translation="MKLTLHEVAKIVDAQNNVSDLDDVPLHHIEFDSRKITKGDLFLP
                     LKGQRDGHEFIDLAFQNGAVATFSEKELPGKPHLLVEDCLKAFQKLAHYYIDKMRVDV
                     IAVTGSNGKTSTKDMIGAVLSTTYKTYKTQGNYNNEIGLPYTVLHMPDDTEKIVLEMG
                     QDHMGDIRLLSEIARPRIAVLTLVGEAHLEYFGSRDKIAQGKMQIVDGMNSDGILIAP
                     GDPIIDPYLPENQMVIRFGNQQEIDVTGIQEDKDSLTFTTNVLATPVSLPLPGKYNAT
                     NAMVAAYVGKLLAVTDEDIIAALQTVTLTGNRTEWKKAANGADILSDVYNANPTAMRL
                     ILETFANIAKNPGGKKIAVLADMKELGKDSVILHSQLIDSLTSGNIDQLVFYGDHIKE
                     LARLASQVYPAEQVHYFLKTEQEDQFEAMAQYVQNILNPFDQILLKGSHSMSLEKLVD
                     RLVTDKNCDSYQP"
     gene            63070..63765
                     /locus_tag="VC394_000061"
     CDS             63070..63765
                     /locus_tag="VC394_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEP5993567.1"
                     /translation="MDFFAIDPIGLPHTSLIFYLSSLLIALLLVFLTFQAYRLKSYRY
                     FFLFLQLSQVIGLYTWYVLRGFPLDEALPLYHCRIAMLAIFFLPDRNKFKQLFMVLGI
                     GGTFLALLSPDLYPFRLWHVANVSFYLGHYALLVNGLIYLLRFYDASQLRLLSVVRYL
                     ATVNFLLLLVSLATKGNYGFVMDIPVIHTRHLLLNFVIVTSGLTFMVKITEYFYLKFG
                     EAQQLAFAFSKEK"
     gene            64013..65557
                     /locus_tag="VC394_000062"
     CDS             64013..65557
                     /locus_tag="VC394_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEP5993568.1"
                     /translation="MSLTEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKSGTFAKSDWMDIEKQRGISVTSSVMQFDYAGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRNIPVFTFINKLDRDGREPLELLEELEEVLG
                     IASYPMNWPIGMGRAFEGLYDLHNKRLELYKGDERFASIEDGDQLFANNPFYEQVKED
                     IELLQEAGNVFSEQAILDGDLTPVFFGSALTNFGVQTFLDTFLEFAPEPHGHKTTEGN
                     VVDPLAKDFSGFVFKIQANMDPKHRDRIAFVRIVSGEFERGMGVNLTRTGKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     PKNVMKQKSFHKGIEQLVQEGAIQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMTPMGKKTVRWISEDDLDQRMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     TLEEKM"
     gene            65864..67483
                     /locus_tag="VC394_000063"
     CDS             65864..67483
                     /locus_tag="VC394_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEP5993569.1"
                     /translation="MKFTEFNLSQDIQSAVVTAGFEKASPIQEMTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLNKIRTNENIIQALVIAPTRELAVQSQEELFRFGREKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALILDHVETLILDEADEMLNMGF
                     LEDIEAIISRVPADRQTLLFSATMPAPIKQIGVKFMKDPEHVQIKNKELTNVNVDQYY
                     VRVKEQEKFDTMTRLMDVNQPELSIVFGRTKRRVDEITRGLKLRGFRAEGIHGDLDQN
                     KRLRVIRDFKNDQIDILVATDVAARGLDISGVTHVYNYDITQDPESYVHRIGRTGRAG
                     KSGESITFVSPNEMGYLSMIENLTKKQMKPLRPATAEEAFQAKKKVALKKIERDFADE
                     TIRSNFDKFKGDAVQLAAEFTPEELALYILSLTVQDPDSLPEVEIAREKPLPFKYVGG
                     GHGNKNGKGGRGRDNRNRGDRRGGYRGDRNRDERDGDRRRQKRDKRDGHDGSGNRDFK
                     RKSKRNSKDFFNKEKKSSAKNTGFVIRHKGE"
     gene            67610..69610
                     /locus_tag="VC394_000064"
     CDS             67610..69610
                     /locus_tag="VC394_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEP5993570.1"
                     /translation="MSDSHLTAFDKASKAGFIIALGIVYGDIGTSPLYTMQSLVENQG
                     GVNQVSESFILGSISLIIWTLTLITTIKYVLIALKADNHHEGGIFSLFTLVRKISPWL
                     IIPAMIGGATLLSDGALTPAVTVTSAIEGLKAVPGLSHIYQNQTNVIITTLVILIVLF
                     GIQRFGTGFIGKIFGPVMFIWFSFLGVSGFFNTLGHLEIFKAINPYYALHLLFSPENH
                     RGIFILGSIFLATTGAEALYSDLGHVGRGNIYVSWPFVKMCIVLSYCGQAAWILANKH
                     SGIELNPFFASVPSQLRVYLVSLATLAAIIASQALISGSFTLISEAMRLKIFPLFRVT
                     YPGANLGQLYIPVINWILFAVTSCTVLAFRTSAHMEAAYGLAITITMLMTTILLKYYL
                     IKKGTRPILAHLAMAFFALVEFIFFLASAIKFMHGGYAVVILALAIVFVMFIWHAGTR
                     IVFKYVKSLNLNDYKEQIKQLRDDVCFDLYQTNVVYLSNRMQDHMIDRSILYSILDKR
                     PKRAQVYWFVNVQVTDEPYTAKYKVDMMGTDYMVRVNLYLGFRMPQTVPRYLRTIVQD
                     LMESGRLPKQEQEYTITPGRDVGDFRFVLIEERVTNARQLSNFERFIMQTKASIKHVT
                     ASPMRWFGLQYSEVTLEVVPLILSDVLKLPIKELVPVEDSEA"
     gene            complement(69634..69912)
                     /locus_tag="VC394_000065"
     CDS             complement(69634..69912)
                     /locus_tag="VC394_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEP5993571.1"
                     /translation="MTTKKAYMYVLECADKTLYTGYTTDLKKRLATHNAGKGAKYTRY
                     RLPVSLLYYEVFDSKEAAMSAEALFKKRKTRSQKLAYIATHQKEKKNH"
     gene            complement(69902..70756)
                     /locus_tag="VC394_000066"
     CDS             complement(69902..70756)
                     /locus_tag="VC394_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEP5993572.1"
                     /translation="MKADFSKVKITLQTFTSSLFYVKITGMIKAILKEGERIDQLFSS
                     DVGIIQNKDVFSYSIDSVLLSRFPKMPSKGLIVDLCSGNGAVGLFASTRTKAAIVEVE
                     LQERLADMGQRSIQLNQLEDQVTMICDDLKNLLNHVPRSGVDLMLCNPPYFKSHESSK
                     KNVSEHYLLARHEVTTNLEEICQVARHALKSNGRLAMVHRPDRFLEIIDSLRANGLAP
                     KRVQFVYPKLGKSANMLLIEAIKDGSIEGMTILPPLVVHKENGEYTDHIFEIYFGAAS
                     KGKPNHDH"
     gene            70796..71536
                     /locus_tag="VC394_000067"
     CDS             70796..71536
                     /locus_tag="VC394_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEP5993573.1"
                     /translation="MFYAYLRGLVVFLLWVVNGNAHYHHEEKMLDASENYILVAPHRT
                     FWDPVYMAFAARPKQFIFMAKKELFANRLFAWWIKMCGAFPIDRDKPSPDAIRYPVNM
                     LKKSNRSLLMFPSGSRHSQEVKGGVAVIAKLAKVKIMPAAYQGPMSVKGLLAGERVDM
                     TFGNPIDVSDIKRMNDEGIAEVANRIQAEFDRMDDELAPFQPGKARNPLTYLYRLPLG
                     LVLVVVLLLTILFSYIASFVWNPDKHRH"
     gene            71736..72398
                     /locus_tag="VC394_000068"
     CDS             71736..72398
                     /locus_tag="VC394_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEP5993574.1"
                     /translation="MLDELLIKGKSFLRERYFLPYLISLLLGLFLILSFSFLFWGNRQ
                     SKAAVPALREISPVKQQVSEEKKEIQEDSSILVDLKGAVQKEGVYKLTASSRVRDVIE
                     LAGGLTSEADKHAINFAEKLTDEQVVYVPKQGEEISVLPRSLVSGKKETASKDQSKVH
                     INKASLEELQHIPGIGAKRAQDIIDMRDKLGGFKALEDLRQVSGIGEKTLEKLKDDIF
                     LD"
     gene            72379..74621
                     /locus_tag="VC394_000069"
                     /pseudo
     CDS             72379..74621
                     /locus_tag="VC394_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989173.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
     gene            74692..75732
                     /locus_tag="VC394_000070"
     CDS             74692..75732
                     /locus_tag="VC394_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEP5993575.1"
                     /translation="MIAIEKIEKLSKENLGLITLVTGDDIGQYSQLKSRLMEQIAFDK
                     DDLAYSYFDMSEAAYQDAEMDLVSLPFFAEQKVVIFDHLLDITTNKKSFLKEKDLKAF
                     EAYLENPLETTRLIIFAPGKLDSKRRLVKLLKRDALVLEANPLKEAELRTYFQKYSHQ
                     LGLGFESGAFEQLLLKSNDDFSQIMKNMAFLKAYKKTGNISLTDIEQAIPKSLQDNIF
                     DLTRLVLGGKVDVARDLIHDLRLSGEDDIKLIAIMLGQFRLYLQLAILAKEGKSEQQL
                     VTSLSEILDRRVNPYQVKYALKDSRTLSLAFLTGAVKTLIETDYQIKTGLYEKSYLVD
                     IALLKIMTHSQK"
     gene            75829..76434
                     /locus_tag="VC394_000071"
     CDS             75829..76434
                     /locus_tag="VC394_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEP5993576.1"
                     /translation="MAIILPELPYAYDALEPQFDAETMTLHHDKHHATYVANTNAALE
                     KHPEIGENLEELLADVTKIPEDIRQALINNGGGHLNHALFWELLSPEKQDVTPDVAQA
                     IDDAFGSFDAFKEQFTAAATGRFGSGWAWLVVNKEGQLEITSTANQDTPISEGKKPIL
                     ALDVWEHAYYLNYRNVRPNYIKAFFEIINWKKVSELYQAAK"
     gene            76601..76827
                     /locus_tag="VC394_000072"
                     /pseudo
     CDS             76601..76827
                     /locus_tag="VC394_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922433.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            76812..77060
                     /locus_tag="VC394_000073"
     CDS             76812..77060
                     /locus_tag="VC394_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5993577.1"
                     /translation="MDADLAQMDALGLYYDDNHLSLVNTVKVYLKEFGIGESQIAFSY
                     KDLNSGRTAAMNDHQPMIAGSTYKLPLNMLVVDKANYP"
     gene            77057..77647
                     /locus_tag="VC394_000074"
     CDS             77057..77647
                     /locus_tag="VC394_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEP5993578.1"
                     /translation="MTERFDITETDYEYDQEHHAYVAQFNGAMSIPDMQEYSLVYSEN
                     TPAYALAERLGGMNKAYQLFDRYGKVSGAITTIDRNGNKITTAYYLQGLDYLWQHQDK
                     YKDILYYIGESFPDLYYKTYLPHVKVYQKPGYVREALNVGAIVCEESPYLITLYSSGL
                     GGATQASEEVNGLGYVQLVQLTYVINEWHRVNHNMI"
     gene            complement(77761..78981)
                     /locus_tag="VC394_000075"
     CDS             complement(77761..78981)
                     /locus_tag="VC394_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3114 domain-containing protein"
                     /protein_id="HEP5993579.1"
                     /translation="MKLLGKWLFFLMFLTFCVSFWQKDYQRIAFEKEHKLYQNLVAPF
                     YQNYPKLRELQLSEAIKLRHDLLDYVRKLDKDGWSYKAIQKGYLEQLAVRGNSYHFEQ
                     LYSRIRLIGSPDFQKLWQQEEMAQTPQEAQKRLQLLLTYLKMPDELTGQSKQTQQVLA
                     QLSPKLSPTDPFWDQLSTLIQAYYVNLEHIPYSRFNRKIHQLRYLISTQQTEWVRSHY
                     GNKGKTDADALAKYLATLEDDDYALYESSRYHNKVASILDANGNHQAVYTDNIPQANY
                     KILIHFHSEFILSESGQFLVALDPDNLTRNSIVNGSSFNYGNQNDDLHRLLDIDPILL
                     FDPAFIEKAINSPDATFLVPDLEQQRDKHNPIYSRNGKSSKQLTRAAVKKFKKLLHHY
                     QETRGDNKTSKTQH"
     gene            complement(78988..80016)
                     /gene="queA"
                     /locus_tag="VC394_000076"
     CDS             complement(78988..80016)
                     /gene="queA"
                     /locus_tag="VC394_000076"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEP5993580.1"
                     /translation="MNTNDFDFELPEELIAQTPLEKRDSSKLLIIDHRQKTMVDSHFD
                     HIIDQLNPGDALVMNNTRVLPARLYGEKPDTHGHVELLLLKNTQGDQWEVLAKPAKRL
                     KVGSQVNFGDGRLKATIIDELEHGGRIVEFSYDGIFLEVLESLGEMPLPPYIHEKLED
                     AERYQTVYAKENGSAAAPTAGLHFTTDLLKKIEAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHDMHSEFYSLSEEAAQTLRDVKQAGGRVVAVGTTSIRTLETIGGKFQGDIQADSG
                     WTNIFIKPGYQFKVVDAFSTNFHLPKSTLVMLVSAFAGRDFVLEAYRHAVDEKYRFFS
                     FGDAMFVN"
     gene            80218..80922
                     /gene="nagB"
                     /locus_tag="VC394_000077"
     CDS             80218..80922
                     /gene="nagB"
                     /locus_tag="VC394_000077"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEP5993581.1"
                     /translation="MKIIRVQDQIEGGKIAFTLLKDSLAKGAKTLGLATGSSPISFYQ
                     EMVKSPLDFSDLTSINLDEYVGLSVESDQSYDYFMRQNLFNAKPFKKNYLPNGLATDI
                     EAEAKRYDQIIAEHPIDFQVLGIGRNGHIGFNEPGTSFEEETHVVDLQESTIKANSRF
                     FTSIEDVPKQAISMGIASIMKSKMIVLLAFGQEKADAIKGMVFGPITEDLPASILQKH
                     DHVIVIVDEAAASQLD"
     gene            81041..81757
                     /locus_tag="VC394_000078"
     CDS             81041..81757
                     /locus_tag="VC394_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEP5993582.1"
                     /translation="MRLDKLLEGTKVGSRSQVKKLIKAQGVWVDHMPARNGRQNVDPG
                     LQLIEVTGQRVTHPKHSYIILNKPSGVVSAKKDTNYLTVIDQLAEEDKSPDLYPVGRL
                     DRDTEGLVLLTDNGPLGFRMLHPIHHVSKTYLVTVNGLLAEDASDFFAAGICFPTGEQ
                     CQPAQLTILKADTDQSQASLTISEGKFHQVKKMFLTYGLKVTSLKRTHFAGLELGHLA
                     SGEYRYLTASERQLIKTYLD"
     gene            82052..83014
                     /locus_tag="VC394_000079"
     CDS             82052..83014
                     /locus_tag="VC394_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEP5993583.1"
                     /translation="MTKILTALDGKNQLISLVTQPISTKPPFCCPACKSPVRLRQGTI
                     RRPHFAHVQLAHCQFQAENESEEHLTLKAKLYTSLVRTEAVCIEKYLPELQQIADLWV
                     NDKLALEIQCSPLPVERLKKRTKAYQEKGYPVRWLLGRKLWLNTHLTALQKQFLYFSS
                     SLGFHLWELDAAANLLRLKYLIHEDLFGKVSYLTKTISLDHNIMEMFRLPYQQEILYS
                     YQKKMTVNLSKRIQRALLARHPKWLRRQEKAYLSGYNLLMLTTDAFYPQWRPVQSSSG
                     FCQIKGNLRPYYESFKVYYKKEKDKKVQTLFSPKYYVKMDSNRK"
     gene            83027..84832
                     /gene="pepF"
                     /locus_tag="VC394_000080"
     CDS             83027..84832
                     /gene="pepF"
                     /locus_tag="VC394_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEP5993584.1"
                     /translation="MTDNRSHLEEKYTWDLSTIFATDKDWEAEVSDLATEIDAAKDFS
                     GHLLDSSANLLTITDTYLELARRVEKVYVYAHMKNDQDTTVAKYQEYQAKASGLYAKF
                     SEVFSFYDPEVMALSQEDYQAFLAETPDLKRYHHFFDKLFQTRDHVLSQAEEELLAGA
                     QEIFNGAEETFSILDNADIVFPVVKNDKGEDVELTHGNFISLMESKDRSIRQAAYEAM
                     YSTYEQFQHTYAKTLQTNVKVQNYKARVHKYDSARQAAMAANFIPEAVYDTLLETVNK
                     HLPLLHRYLKLRQEVLGLDDLKMYDVYTPLSETDLAIGYDEALEKAEKVLAVFGKDYA
                     DRVHRAFTERWIDVHVNKGKRSGAYSGGSYDTNAFMLLNWQDTLDNLYTLVHETGHSL
                     HSTFTRETQPYVYGDYSIFLAEIASTTNENIMTEALLNEVQDEKERFAILNHYLDGFR
                     GTVFRQTQFAEFEHAIHQADQKGEVLTSEYLNQLYADLNEKYYGLSKKDNHFIQYEWA
                     RIPHFYYNYYVYQYATGFAAASYLADKIVHGTQDDIDHYLAYLKSGNSDYPLEVIAKA
                     GVDMAKGDYLEAAFKVFDERLTELEVLVSKGIHKS"
     gene            85208..86404
                     /locus_tag="VC394_000081"
     CDS             85208..86404
                     /locus_tag="VC394_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEP5993585.1"
                     /translation="MEKTKRYIIATAGILLHLMLGSTYAWSVYRNPILQETGWDQAPV
                     AFAFSLAIFCLGLSAAFMGNLVEQYGPRLTGTVSAILYASGNMLTGLAIDRKEIWLLY
                     IGYGVIGGLGLGAGYITPISTIIKWFPDKRGMATGFAIMGFGFASLLTSPIAQWLIET
                     EGLVATFYLLGLIYLIVMLFASQLIIKPTAAEIAILDKKRLQNNSYLIEGMTAKEALK
                     TKSFYCLWVILFINITCGLGLISVVAPMAQDLTGMSPEMSAIVVGAMGIFNGFGRLVW
                     ASLSDYIGRRVTVILLFLVSIIMTISLIFAHSSLIFMISIATLMTCYGAGFSLIPPYL
                     SDLFGAKELATLHGYILTAWAIAALTGPMLLSITVEWTHNYLLTLCVFIVLYILGLMV
                     ALRLKK"
     gene            86470..87177
                     /locus_tag="VC394_000082"
     CDS             86470..87177
                     /locus_tag="VC394_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEP5993586.1"
                     /translation="MVKSYSKTANHNMRRPVVKEELVHYMRTRQKQITGFLAELEQFA
                     RQENIPIIQPEVVAYFRFLLQSLQPKHILEIGTAIGFSALLMAENAPDATIVTIDRNR
                     EMIDFAKANFAKYDSRQQIRLLEGDAADILSTLEGNFDFVFMDSAKSKYIVFLPEILR
                     LLKVGGVVILDDVFQGGDITKPIEDIRRGQRTIYRGLQSLFDATLTHPNLTTSLVPLS
                     DGLLMIRKNQADILLPD"
     gene            87240..88295
                     /gene="prsA"
                     /locus_tag="VC394_000083"
     CDS             87240..88295
                     /gene="prsA"
                     /locus_tag="VC394_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEP5993587.1"
                     /translation="MKNSNKLIASVVTLASVMALAACQSTNDNTKVISMKGDTISVSD
                     FYNETKNTEVSQKAMLNLVISRVFEAQYGDKVSKKEVEKAYHKTAEQYGASFSAALAQ
                     SSLTPETFKRQIRSSKLVEYAVKEAAKKELTTQEYKKAYESYTPTMAVEMITLDNEET
                     AKSVLEELKAEGADFTAIAKEKTTTPEKKVTYKFDSGATNVPTDVVKAASSLNEGGIS
                     DVISVLDPTSYQKKFYIVKVTKKAEKKSDWQEYKKRLKAIIIAEKSKDMNFQNKVIAN
                     ALDKANVKIKDKAFANILAQYANLGQKTKAASESSTTSESSKAAEENPSESEQTQTSS
                     AEEPTETEAQTQEPAAQ"
     gene            88682..91300
                     /gene="alaS"
                     /locus_tag="VC394_000084"
     CDS             88682..91300
                     /gene="alaS"
                     /locus_tag="VC394_000084"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEP5993588.1"
                     /translation="MKELSSAQIRQMWLDFWKSKGHCVEPSANLVPVNDPTLLWINSG
                     VATLKKYFDGSVIPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAIEWGFELLTSPDWFDFPKDKLYMTYYPDDKDSYNRWIACGVEPSHLVPIEDNFWEI
                     GAGPSGPDTEIFFDRGEDFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPNKNIDTGAGLERLAAVMQGAKTNFETDLFMPIIREVEKLSGKTYNPDGDNMS
                     FKVIADHIRALSFAIGDGALPGNEGRGYVLRRLLRRAVMHGRRLGINETFLYKLVPTV
                     GQIMESYYPEVLEKRDFIEKIVKREEETFARTIDAGSGHLDSLLAQLKAEGKDTLEGK
                     DIFKLYDTYGFPVELTEELAEDAGYKIDHEGFKSAMKEQQDRARAAVVKGGSMGMQNE
                     TLAGIVEESRFEYDTYSLESSLSVIIADNERTEAVSEGQALLVFAQTPFYAEMGGQVA
                     DTGRIKNDKGDTVAEVVDVQKAPNGQPLHTVNVLASLSVGTNYTLEINKERRLAVEKN
                     HTATHLLHAALHNVIGEHATQAGSLNEEEFLRFDFTHFEAVSNEELRHIEQEVNEQIW
                     NALTITTTETDVETAKEMGAMALFGEKYGKVVRVVQIGNYSVELCGGTHLNNSSEIGL
                     FKIVKEEGIGSGTRRIIAVTGRQAFEAYRNQEDALKEIAATVKAPQLKDAAAKVQALS
                     DSLRDLQKENAELKEKAAAAAAGDVFKDVQEAKGVRFIASQVDVADAGALRTFADNWK
                     QKDYSDVLVLVAAIGEKVNVLVASKTKDVHAGNMIKELAPIVAGRGGGKPDMAMAGGS
                     DASKIAELLAAVAETV"
     gene            91661..91876
                     /locus_tag="VC394_000085"
     CDS             91661..91876
                     /locus_tag="VC394_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP5993589.1"
                     /translation="MEVILKNRLKELRARDGINQTEMAKLAGVSRQTISLIERNEYTP
                     SVIIAMKIAKVFQEPVEEVFRLVEVEE"
     gene            91873..92634
                     /locus_tag="VC394_000086"
     CDS             91873..92634
                     /locus_tag="VC394_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEP5993590.1"
                     /translation="MKKKKNSFLRLLKMLLLSSLAGGIIGGMVGAFLGYHGERLDQLT
                     FLKDDVINLIILLNRLVVVTGLTLSFVFLTQLKKETAVYNTIEEDDYSENGYRQLNKK
                     HAYTMLLIAVASILSMCNVLLGLTLTNDSQHAMLAIPLLDILLLLMVIPFQALAMKRY
                     NAIRGTDVPYFPNLKELKHNIMALDEAELQAYHKTSFESVLSLNGVIIPSLYVILFFV
                     YLFTGQVELTAILVLVLIQLYLLVKSATMTRQFYR"
     gene            92653..93348
                     /locus_tag="VC394_000087"
     CDS             92653..93348
                     /locus_tag="VC394_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEP5993591.1"
                     /translation="MKGFINYLKIAVLIILAMVFNVLPMILLQKQHDIPMVLNWGIGI
                     FYLVIVGSVLIVLWRLYQAKQDTPIKQQKMRLVDWGYLALFWLIIRVIAIAGTLVNQL
                     WSGQQVSANDAAIHTLARLIKGGFPLYTALFVLVIAFIAPIMEELVFRGFPMIDLFKG
                     KSLKVAGLVTSLVFALPHATNSVEFIMYSCMGIFLFVAYQRRGNLKDAILLHIFNNLI
                     EVILLMSIGLGVI"
     gene            complement(93442..93969)
                     /locus_tag="VC394_000088"
                     /pseudo
     CDS             complement(93442..93969)
                     /locus_tag="VC394_000088"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT        29346 a        16322 c        19652 g        28715 t
ORIGIN      
        1 taatttataa gtataaattt ttacactaaa atgtaaaaaa catacaagta aacgaataca
       61 aatctataaa aataaaaata acgccccgaa aatgtaagcg attactcaaa atagctgtga
      121 gaaataccct atttttatct tttttaagga gtacactagg aatataagca agagttaaga
      181 atcttgacgg tgaattcaca aaaaccaatt agaaagaggt aattcttatg actgctcaaa
      241 caccaattca tgtttattct gaaattggaa aattaaaaaa agtgttgctc cacagacccg
      301 gtaaagaaat tgaaaatctt atgccagact acttggaacg cttgttgttt gatgatattc
      361 cattcttaga ggatgcccaa aaagaacatg atgcttttgc ccaagctttg cgtgacgaag
      421 gtattgaagt cttgtacctt gagaccttag ctgcagaatc tcttgtcact ccagaaattc
      481 gcgaagcttt catcgatgaa tacctaagtg aagctaatat ccgtggaaga gctactaaaa
      541 aagccattcg tgaattgttg atggctatcg aagacaatca agaattgatt gaaaaaacca
      601 tggcaggagt tcaaaaatct gaacttccag agatcccagc atctgaaaaa ggcttgactg
      661 acttggttga atccaattac ccatttgcga tcgacccaat gccaaacctt tatttcacac
      721 gggacccatt cgcaactatc ggtacaggtg tttctcttaa ccacatgttc tcagaaacac
      781 gtaaccgtga aaccctttat ggtaaataca ttttcactca tcacccaatt tatgggggtg
      841 gcaaagtgcc tatggtttat gaccgtaatg aaaccactcg cattgaaggt ggggacgaac
      901 ttgttctttc aaaagatgtg cttgcggttg gtatttctca acgtacagat gctgcttcta
      961 ttgaaaaatt gttggttaac atctttaaac aaaaccttgg cttcaagaaa gtattggcct
     1021 ttgaatttgc aaataaccgt aaattcatgc acttagacac tgtctttacc atggttgact
     1081 atgacaaatt taccattcac ccagaaattg aaggcgacct tcgtgtttac tctgtcactt
     1141 acgacaatga agaacttcat atcgttgaag aaaaaggtga tttagcagaa cttcttgctg
     1201 ctaaccttgg tgttgaaaaa gttgacctta tccgttgtgg tggtgacaac ttagtagcag
     1261 caggtcgtga acaatggaac gatggttcta acacccttac tatcgcacca ggtgtggttg
     1321 tggtttataa ccgtaacacc attaccaatg ctattcttga atctaaaggc ttgaaattga
     1381 tcaagattca cggaagtgaa ttggttcgcg gtcgtggtgg acctcgttgt atgtcaatgc
     1441 catttgaacg tgaagatatt taataagcta tggtaaaggt ggttataggt cagaagcctt
     1501 tttaaagggc agctagtgtt tatcttcgct tctgtgtctt tgtccttcaa tgaaattttg
     1561 tcatgacaga taaatttgat gccaatgacg aaacaagaac ggtttatgca gtcgtttatg
     1621 acaatgacca gcccgtttca acaggacaat ttttagctga aacgaaaata gaagcacgat
     1681 tgacacgcat tgtaacctta gcagattatt gtggttgcgg ttatggtgcc aaagtcactg
     1741 aagcgctaga aacttatacc agacgagaag gcttttacca actaaccatt cacagtgaac
     1801 tgacagcaca aaccttttat gaaaacctag gttatcagtc ctatggtccc aagtgtttag
     1861 aagatggtga gtattgtcag tcccttgcaa aaaccattct taaatgggag aagaatatgg
     1921 acatagcaat gctaattgcg attgttggtg gtctattagg ctgctatctc tatctcacaa
     1981 aaaataatta atcaaaacag attacagtta acactcgaag gagacaatag atgacacaag
     2041 tatttcaagg acgtagcttc ctagcagaaa aagattttac acgcgctgaa ttagaatacc
     2101 ttattgattt ttcagcacat ttgaaagatt tgaaaaaacg tggtgtgcct catcactact
     2161 tagaaggtaa aaacattgcc ctcttgtttg aaaaaacatc aactcgtacg cgtgcagctt
     2221 ttacaacagc agccattgac ctaggtgctc acccagaata cctcggtgcc aatgacatcc
     2281 aacttggtaa aaaagaatca acagaagaca ctgctaaagt attgggtcgt atgtttgatg
     2341 ggattgaatt ccgtggcttt agccaacgca tggttgaaga attggcagaa ttctcaggtg
     2401 tgccagtttg gaatggtttg actgatgaat ggcacccaac ccaaatgctt gctgactatt
     2461 taacagtgaa agaaaacttt ggcaaacttg aaggccttac ccttgtttac tgtggtgacg
     2521 ggcgtaataa cgttgccaat tcccttcttg tgactggtgc tatccttggt gttaatgttc
     2581 atatcttctc accaaaagaa cttttcccag aagaagagat tgtgacattg gctgaaggct
     2641 atgctaaaga aagtggcgct cgtatcctta tcacagaaga tgctgacgaa gctgttaaag
     2701 gcgcagacgt gctttacaca gatgtttggg tatcaatggg tgaagaagac aaattcaaag
     2761 aacgtgttga attgcttcaa ccatatcaag tcaacatgaa ccttgttcaa aaagcaggaa
     2821 atgacaagct tatcttcttg cactgcttgc cagctttcca tgacacaaat accgtttatg
     2881 gtaaagacgt tgctgaaaaa ttcggcgtga aagaaatgga agtcactgat gaagtcttcc
     2941 gcagcaaata cgctcgtcat tttgaccaag ctgaaaaccg catgcacact atcaaagcgg
     3001 ttatggcagc aacccttggt aacctcttta ttccaaaagt gtaataagga ctggtactcc
     3061 ttaactcatt tcacatatca atgtgacccc tttaatttaa caatttaact acttaaaaac
     3121 gtactttaac tacatgacaa gtcgttctgg ttttcgcgtc cttgaccgga actagaacgg
     3181 cttattttta tgaaaaggta agaggtaatc attatgacag aagaaaaaaa acggggtttt
     3241 agaattcctt cttcttacac cgttcttttt atcattatag ccatcatggc agtgttaact
     3301 tggtttattc cagctggcgc ctacgaaacc gctaagggcg gtggtgttat ttcaggaacc
     3361 tacaaaacag tagcatccaa tcctcaagga ttttttgaca tcttgatggc tcccgttcga
     3421 ggcatgttag gtgttgaagg cactgatggt gctattcaag tgtctttctt tatcttaatg
     3481 gttgggggct ttttaggagt tgtcaacaaa acaggtgctc ttgacacagg gattgcgtca
     3541 gtggttcgta aaaacaaagg tagagaaaaa atgctgattg ctattttaat ccctttgttt
     3601 gctctaggtg gaacgaccta tggtatgggg gaagaaacca tggcctttta cccactttta
     3661 attcctgtta tgattgcggt tggttttgac agtattgttg ctgtagccat tattttgatt
     3721 ggttctcaaa ttgggtgtct agcctccact attaacccat ttgcaactgg tgtagcagct
     3781 gatgctgctg gtgtcagcat tgcagatgga atgatttggc gtgttatcca atgggttatt
     3841 cttgtcggca tgtctatttg gtttgtttac aattacgcta gtaagattga agaagaccca
     3901 agtaaatcat tggttgcaga caaggaagaa gagcataagg aactcttcca gttgcaaaat
     3961 tctggagaag acttaaacaa gcgccaacga aacgttttga ccatttttac tttgaccttt
     4021 gtcattatga ttcttagttt gattccgtgg gaagattttg gcatcaaatt ctttaccaat
     4081 atcaatactt ggttaaccac aatgcctatc ttagggggag ttattgggaa aaccatggga
     4141 gcatttggta cttggtattt cccagaaatc accatgctct ttatcatgat gggtgtctta
     4201 gttgctattg tttatcgtat gagtgaagaa gactttttta gctctttttt gactggtgca
     4261 ggtgaatttt taggtgttgc catgatctgt gctattgcac gcggtattca agttatcatg
     4321 aatggtggta tgattacagc cactatctta cacttaggtg aaacaagtct ttctggttta
     4381 tcttctcaag tttttgtgat attagcttat attttctacc tcccaatgtc cttcttgatt
     4441 ccatcaacat caggacttgc tggggctaca atggggatta tggcaccgct tggacaattc
     4501 tcaaatgtcc ctgctcacct tgttattaca gccttccagt cagcttctgg aatcttaaac
     4561 atgatttctc caacttcagc aatcgttatg ggagcacttg cgcttggtcg cgttgacctt
     4621 ggtacttggt ggaaattcat tggtaaattt attgtaatgg tgatgcttgt aagcgtgcta
     4681 ttacttgtag ttgcaacatt cttttaacag ataggaggtt cctatggaat cctatatcac
     4741 acctaaacat caagaggcct gtgtagctgc cattaaacag atcgtttctt acccttctgt
     4801 ttgtcatgaa ggagaaaatg gcacaccttt tggacaggcc attcaagatg ttctcgaagc
     4861 aaccttagac ttatgtcaag gtcttggttt tcatacttat attgatcctg aaggttacta
     4921 tggctatgct gagttaggtg accaaaaaga ggtcttggct attctttgcc atttggacgt
     4981 tgttccagaa ggagatcgtc agttatggaa gacagatcct tttgactgtg ttgaggcaga
     5041 cggctgttta tttggtcgag gcactcaaga cgataaaggg cctactatga tggcgctatt
     5101 tgccaccaaa gccttgatag atgcaggtgt tacctttaat aaacgcattc gttttatttt
     5161 cggaacagat gaagaaactt tgtggcggtg tatgaatcgc tacaatgatg tagaagaaca
     5221 ggctaccttt gggtttgctc ctgattcaag tttcccttta acctatgccg aaaaaggcct
     5281 acttcaggca aaattagtgg ggaaaggttc tgataaactg tcacttgaaa tcggacaagc
     5341 ctacaatgtt gttcctgcaa gagcttctta taaaggcaat aagcttgagg ctttacaaaa
     5401 agaactagat aagcttggct ttgagtacgt cgtcaaagag ggagaattaa cggtttatgg
     5461 tctcgcccaa catactaaag atgctcccga tggcatcaac gctatgatta ggcttgccaa
     5521 ggctttagtg gtccttgaac ctaacaaaac ccttgatttt cttgctaatg tcattgatga
     5581 agatggcaga gcacttaata tttttggcag aatcgaagat gaaccttcag gcaaattaag
     5641 ctttaatgcc gcagggctta ccttaaccaa agacaaagca gaaattcgcc tagatattcg
     5701 gattccagtt ttggcagata aggaaaaatt agtccaacaa ttaagccaaa aagcacaaga
     5761 atacggccta acctatgaag aatttgatta tttggcccca ttatatgtgc cattagacag
     5821 tgaattggta acaaccttgt tatctgtcta tcggaaaaaa acaggtgatc aaagccctgc
     5881 ccaatcatca ggaggggcaa cctttgctag aacaatgaag aattgtgttg cctttggtgc
     5941 ccttttccct gatgctgttc aaacggaaca ccaagaaaat gaacatattg ttttagcgga
     6001 tgcttaccgc gctatggata tttatgcgga agctatttac cgactaacgc gataacaaga
     6061 tatgaggagt aattatgacg aaacaaaaaa tcgtagtcgc acttggagga aatgctattc
     6121 tttcaacaga tgcgtcagca aaagcacaac aagaagcctt gatttccaca tcaaaatcat
     6181 tggttaaatt aattaaagaa ggtcatgaag tcatcgtgac ccacggcaat ggcccacaag
     6241 ttggtaatct cttattacaa caagcagcgg cagattctga aaagaatccg gctatgcctt
     6301 tggacacttg tgttgccatg acagaaggta gcatcggttt ttggttagtt aatgcccttg
     6361 ataatgaatt gcaagcccaa ggtattcaaa aagaagtggc agctgttgtc actcaagtga
     6421 tcgtggatgc caaagaccca gcttttgaaa acccaacaaa accaattggt ccatttctta
     6481 ccgaagaaga tgccaaaaaa caaatggcgg agtctggtgc aagtttcaaa gaagatgcag
     6541 gtcgtggctg gcgtaaggtt gtgccatcac caaaaccagt tggtattaaa gaagccaatg
     6601 tcatcagaag tctcgttgat tctggcgttg ttgtggttag tgctggtggt ggcggtgtgc
     6661 ctgttgtgga agatgcaact agcaagtcct tgactggtgt ggaagctgtt attgacaagg
     6721 actttgctag ccaaaccctt tcagaattgg tggatgcaga cctctttatc gttttgacag
     6781 gagttgacaa tgtttacatc aacttcaaca aacctgacca agccaaattg gaagaagtga
     6841 ctgtctctca aatgaaagaa tacatcacac aagaccaatt tgcaccaggt agtatgttac
     6901 caaaagtgga agctgccatt gcttttgttg aaaacaaacc aaatgctaaa gccattatca
     6961 catcgctaga aaatattgac aatgtactat cagcaaacgc tggaacacaa attatcgcag
     7021 ggtaatcaag aggattttta gttctgttta aaagactcat ttaatggtat tttctgaaga
     7081 gtacgactga cataaagagt tgatactgtt tttaaaggaa aagagtgtgc cataataaaa
     7141 aacaatagta gtattagaaa aggctaattt tattgagaat tagccttttc ttttagcttt
     7201 gataggtgta taataaccat actatttttt atttttcaaa atattagaat aaacaaaagt
     7261 ggacaacgta taaagagaac tgctaagtcg ttgagttcat tagtcccaaa aagattacca
     7321 aaaatgaagg aggagctatg aagaaaagtt ttattcatca acaagaggag atttcctttg
     7381 ttaagaatac gttcactcag tatttgattg ctaagttaga tgttgttgaa gtgcaaggac
     7441 ccattttaag tcgggtcggg gacggtatgc aagataattt gtccggtact gaaaatccag
     7501 tatctgtcaa tgtcttgaaa attcctaatg caacctttga agttgtgcat tcattagcaa
     7561 aatggaaacg tcacacctta gcccgttttg ggtttaatga aggtgaaggc cttgttgtta
     7621 atatgaaggc tctccgacct gatgaggatt cacttgacca aacccattct gtctatgttg
     7681 accaatggga ctgggaaaaa gtcatccctg atggcaaacg taatctcgct tacctcaaag
     7741 aaaccgttga aaccatttac aaggtcattc ggttaactga attagctgta gaagctcgtt
     7801 atgatattga ggctgtttta cctaagaaaa taaccttcat tcataccgaa gaattggtag
     7861 ctaaataccc tgatttgacg cccaaagaac gtgaaaatgc cattactaaa gaatttggag
     7921 cagtcttttt aattggtatt ggcggggttt tacctgacgg taaaccacac gatggccgcg
     7981 ctcctgacta tgatgactgg acaacagaaa ctgaaaatgg ctatcatggg cttaatggtg
     8041 atattttggt ttggaatgac caacttggtt cagcctttga attatcctca atggggatcc
     8101 gtgtggatga agaggcccta aaacgtcagg tggaaatgac tggtgaccaa gaccgcttag
     8161 cctttgattg gcacaaatct ttgttgaatg gacttttccc tctaacgatt ggtggaggaa
     8221 tcggtcaatc acgtatggtc atgtttttgt tgcgtaaaaa acatattgga gaagttcaga
     8281 cctctgtttg gccccaagaa gttcgtgata gctacgataa tattttataa gtaatcacat
     8341 cattttgcta agttttccac ttgtataaga gactttaaat aaatgatcag aaatattgaa
     8401 aaaagaaatc atttttaagg tttagagcta gaaatttgct ataataaata ttatgagagt
     8461 tgtatcaggt gaatttggtg ggcgtccttt aaagacgctc gatggaaaga taacacgccc
     8521 tacttcagac aaagttagag gtgcgatttt taatatgata ggaccttact ttaatggtgg
     8581 tcgtgtttta gatttgtttg caggttcagg tggattagcc attgaggctg tttctcgtgg
     8641 gatgtctgct gcagttttgg ttgagaagaa tcgtaaagcg caagctatta ttcaagacaa
     8701 tattataatg actaaagctg agaatcggtt taccttgtta aaaatggaag ctgagcgagc
     8761 tattgattgc ttgactggtc gatttgactt agtcttttta gaccctcctt atgccaaaga
     8821 aacaattgtg gcaactattg aagctttagc agctaaaaac ttattgagtg aacaggttat
     8881 ggttgtctgt gagacagata aaacagtctt gttgccaaaa gagattgcaa cgttaggtat
     8941 ttggaaagaa aaaatttatg gaattagtaa ggtaacagtt tatgttaact aaaataggac
     9001 tttatacagg gtcatttgat cctgttacaa acggtcatct cgatattgtt aaacgggcaa
     9061 gtggcctatt tgatcaaata tatgttggga tttttgataa tcctaccaaa aaaagctatt
     9121 ttaaactaga agttcgtaaa gcaatgctta ctcaagctct ggctgatttt acaaatgtaa
     9181 ttgtagtaac ttcgcacgaa cgtttagcta ttgatgtagc taaagaatta cgagtaactc
     9241 acttgattag aggcttgcga aatgcaactg actttgagta tgaagaaaat ttggaatatt
     9301 ttaatcacct tttagctcca aatattgaga ccgtctattt gatatcaaga aataaatggc
     9361 aggcccttag ctctagtcgt gtgagagagc taattcattt tcagtcttcc ttagaaggtt
     9421 tggttcctca gtcagttatt gctcaagtgg agaaaatgaa tgaaaagact taaaaaaatc
     9481 aaatggtggt tagtgggtct gctagcttta atctctttgt tgctagcgtt attttttccg
     9541 ctaccttatt atattgaaat gcctggaggc gcttacgata ttcggactgt cttacaagtc
     9601 aatggcaaag aagacaaacg aaaaggagct taccagtttg ttgcagtggg cattagtcgt
     9661 gccagcctcg cgcagctatt atatgcttgg ctgacaccgt ttactgaaat tagtacagca
     9721 gaagatacaa caggcggata cagcgatact gatttccttc gaattaatca attttacatg
     9781 gaaacatcac aaaatgcagc tatttatcaa gctttatcct tagctggaaa accagttaca
     9841 ttagattata aaggcgtata tgttttagac gtaaacaacg aatctacttt taaaggaacg
     9901 ttacacttag cagatactgt aacaggtgta aatggtaaac agtttactag ttcagcagaa
     9961 cttattgact atgtttctca cctaaaacta ggggatgaag ttacggttca gtttacgagt
    10021 gataataagc ctaaaaaagg agttggccgt attatcaaac tgaaaaatgg gaaaaatggg
    10081 attggcattg ccttgactga tcatacaagt gtcaattcag aagacacagt gatctttagt
    10141 actaaaggag taggaggacc tagtgctggt ctaatgttta ctcttgatat atatgatcaa
    10201 ataactaaag aagatttacg caagggccgt acaattgcag gtacaggaac tattggcaag
    10261 gatggcgaag taggagatat tggtggtgca ggtcttaaag tagttgcagc agctgaagct
    10321 ggtgcagata tattttttgt tccgaataat cctgttgata aggaaattaa aaaagttaat
    10381 ccaaatgcta taagtaatta cgaagaagcc aaacgggcag ccaaacgact aaagaccaaa
    10441 atgaagattg ttcctgttac gactgttcaa gaggcactgg tttatcttcg caaataaaat
    10501 aactatgaat gtattatatt caattatatt cacgctatta actcataaaa aaacggttga
    10561 accagataga cagatccttg accagtcttt tgggttcaat cattttttac ataaaacata
    10621 tctaacttga cagcgtaaaa aagatgttat aaaagtctag tggtaaaaag gcaatagaga
    10681 attgagtaaa ctttagtcga ttatctggaa atagagcata ctaaaaacac ctagtgaaaa
    10741 ttgatttttt tcactagctc atccaagaac ttgcgcagat aatcatgagt ccattccatg
    10801 actctattat tctggaaagc cttgcaatag acaagctaac gttataacaa ctaaattaaa
    10861 aacttgagat tgaaaaatcc ccataaactt attttacaag gaggtaaggc tatggtaacc
    10921 attaaacaag ttgctgaaga agcaggtgtt tctagatcaa cagtctccag gtatatttct
    10981 caaaaaggat atgtatctga tgatgcaagg cataaaatca aagcagcgat agcaaaatta
    11041 cattacactc caaatgtctt ggcgcagtca ttgaagacga aaaaaaatca actagtcggt
    11101 ttattattac cagacatttc caatcctttt tttccgaggt tagcaagagg agcagaagaa
    11161 tatttaaaag aaaaggggta tcgggttatg ctaggaaata tttcagacag cgaagctctt
    11221 gaagaagaat acgtccatgt ccttcttcaa agcaatgctg ctggcattat tactacccat
    11281 gactttacaa agcggtatcc tactcttgct attccagttg tagtggtaga ttgtgttgac
    11341 caagagaccc agtacggtgt cttttcagat aatagggcag ggggcttatt ggcggctcag
    11401 actgtctggc aagcaggggc gaaagaagtt ctgttaataa gaggtccgct ggataatgct
    11461 gaaaacatta atgagcggtt tgaagctagt ttttcttacc ttcaaaaaca agacgtgact
    11521 atgtatgtgt gtgatagtca gaattttgat tttgaaagta ttcagctaga agctagttac
    11581 aacctcaagt gttacccaac aatagatagt attatagcac cttctgatat ccatgctatt
    11641 gcttatattc atgaactgca ttctcaaggt aaaaagattc ctcaagatgt tcaaataata
    11701 ggttatgatg acattttaat gagtcaattt atttatcctt ctttatcgac tattcatcaa
    11761 tcctcttatt tgatgggccg ttatgccgcc gaattagttt atactattgc tagtcaatta
    11821 actgtgaaag caaatcggat taaattgcct gttcactacg ttgagcgaga aacaataagg
    11881 aaaagataag taataatatt agtccagatt ttagcaggta tgatgaagat ttattgggat
    11941 ttaccttcta ctttaaaaga tgttatctgt cattactttc atcgctatca gtcaaaacgc
    12001 attcttatgc aactcttaac cttaagctta atgtcttttt gataacatag aggaatgtta
    12061 cacgatgatg acgcaataaa gcctaaatat agtaattgtc gtgattttat agctagtgag
    12121 taatgagata gcaagtctct catttactta caaaagtcaa aaaatagtgt ataatggttg
    12181 agttacagat aacactgcaa aaaagataac aagtgtccat aattatctaa gactataaag
    12241 gcgatataga cagatataaa aaagatgacg aatcagttat cactggacaa ttaaccgatt
    12301 tagttacaac taatggattt ttcagaaagg tgtcaaatga aaaaagaaat ttcgccagaa
    12361 atgtacaact ataataaatt tccaggtccc aaatttattc attttgaaga gcaagttaag
    12421 gctgaaggca ttgatttgtt actcttagaa gatgttaaga acgcttttga tacgactagc
    12481 tttggtcaac gttatacaga agttctccta aagtatgact atattgttgg cgattgggga
    12541 aatgaacagc ttcgtcttaa agggttttat aaggatagtg atgatatcaa gaaaacgaat
    12601 cgcatctcac gtttagaaga ttatattaaa gaattttgca attttggttg tgcttatttt
    12661 gttttagaaa atcttcatcc acaagatatt aaatttgaag aggagcgcca accaagacga
    12721 aagaaatcac ctaaatcaaa atcaaatcgt cgcaagccaa actattcaaa tcagcagccc
    12781 gcaacaccta agagtaaatc gaagcgggta tcaaaagaaa agcaacctga aaaccaagcg
    12841 tttaccagtc aaaaacgtcg aagtaatact aagcataaag aaaagtcaaa acgtaatcag
    12901 actagtcaac ttaataccaa aattagtcat tttatcatca gaaagaaaga taaataatcc
    12961 atgaaaccat cgatttatag cttaacacgc gatgaactga tcgcgtgggc agttgaacgc
    13021 ggccaaaaac agtttagagc tactcaaatt tgggattggc tctataaaaa aagagttcaa
    13081 tcttttgagg agatgacgaa tatttctaaa gattttgttt ctattttaaa tgatagtttc
    13141 tgtgtcaatc ctttaaaaca gcgagttgta caagagtcag ctgatggtac agtaaaatat
    13201 ttatttgaac tgcccgatgg tatgttaatt gaaacagttt taatgcgtca acattatggt
    13261 cattctgttt gtgtgacgac tcaagttggc tgtaacattg gctgtacttt ctgtgctagt
    13321 ggattgatca aaaagcaacg tgatctaaac agtggggaaa ttacagctca aatcatgtta
    13381 gtccaaaaat attttgacga tagaaagcaa ggtgagcggg ttagccatgt tgtcgtaatg
    13441 ggcattggtg aaccttttga taattacaaa aatgtcatgt gctttcttcg cgtcatcaac
    13501 gatgataatg gcttagcaat tggtgcccgt catattacag tctctacctc agggttagct
    13561 cacaagattc gggattttgc aaacgaggga gtacaggtta atttagcagt ctcgttgcat
    13621 gcaccaaata atgatttacg ttcaagtatt atgcgagtaa atcgctcttt tccgctggaa
    13681 aagctctttt cagctataga atactatatt gaaaaaacga atcgtcgtgt cacttttgaa
    13741 tacattatgc taaatgaagt taacgatagc attaagcaag ctcaagaatt agcagactta
    13801 actaaaacta ttcgtaagtt atcttatgtt aacttgattc cgtataatcc tgtttcagaa
    13861 catgatcagt atagtagaag tcctaaagag cgagttctag cgttttatga tgtgctaaag
    13921 aaaaatggag ttaactgtgt ggtgcgtcaa gagcacggaa cagatattga tgcagcctgt
    13981 ggccagttac gttctaaaac gatgaaaaaa gatcgcgaaa aggttacagc gacaaaataa
    14041 agcccaaaaa atatctttga ttgacaatgc ttgatgacaa aatattccta gtcactaaaa
    14101 tgactaggtt ttttatctgg cctaaaaatg ctctagaagc acaatccaaa ataatccaaa
    14161 actaagataa ggaataaaag gtatgcgctt gatgcctaat agtaagcaag cagtaatacc
    14221 tgctaaacta gcaagttgaa ttagccaaat caacgacgtt agatccaata ctaaagctaa
    14281 gctagcaagg tagaaaaaat cgcctgctcc aattgaaaaa ggtcttaaat gagctaataa
    14341 acctagtagc agtaatatca atgataaata attgatcgaa taaaacagga gtagacttac
    14401 aaagctaaaa agccacaaga taaaagggta ttcttgagaa cgccaatcaa ataaactaaa
    14461 taataaagaa aagagtaaca agtaacagta actagcacta acccaatgta ccatactagc
    14521 ccataagata attattagac ttatctctaa gctaaagaag attttttggc tagttataaa
    14581 atggttttgg agctgccgaa aaacgcgttt ggcaaaagtt agttggtcac aaacaatgac
    14641 agtctgttta cttagctgtt cttgaccatt atcaggaaaa taaagtagta aacagtaaaa
    14701 aagatgggtc aataagatgc ccgaacatat actaaaaaaa gctatcatac ttatttattc
    14761 gaaaagaaat caaatacttg caatctttcc ttaatcattg tagaatatag atataaaacg
    14821 ataatgattc taattaagtg tctaatcatc caattaggat tattccgtta ttgtcgaata
    14881 ggattaggtc cttaaccctt tcttatgttt atcacgttag ggttagttcc ccctccattc
    14941 ttgcccttta agctgaaaag taaggagatg ttattatgac aaacacactc gttgaaaata
    15001 tttatgcttc agtaactcac aatatttcta aaaaagaagc atcaaaaaat gaaaaaacta
    15061 aagctgtttt aaaccaagct gtcgctgact tatctgtggc tgcatctatt gtgcaccaag
    15121 tacactggta tatgcgtggt ccaggtttct tgtaccttca cccaaaaatg gatgaattac
    15181 ttgatagctt gaatgctaat ttagatgaaa tgagcgagcg tttgatcact attggcggag
    15241 cgccttattc taccctagct gagttttcta aacactctaa attggacgaa gcaaaaggta
    15301 cttacgataa aacagttgct cagcatttag caagattggt cgaagtttac ctttacctca
    15361 gcagccttta ccaagttggt ttggatatta ccgatgaaga aggcgatgct ggaacaaacg
    15421 atttgtttac agcagctaag acagaggctg aaaaaaccat ttggatgctc caagcagagc
    15481 gtggacaagg cccagcactc taatggatta tgccttagga taaaagaagt caatgataaa
    15541 attggcttct tttttatgga atcatcgatt cattttcaga aacatagaaa gcgattccat
    15601 acttgtcttg aaatttgccg tagaatttgc taaaatctac ttatatgaaa acactatatg
    15661 atgtgcaaca attgctaaaa aactttggca tttttgttta ccttggcaaa cgtctctatg
    15721 atattgaaat gatgaagatt gaacttcagc gtctctatga tagcggctta ttggataaga
    15781 gggattactt aaatgcggag ttaattttgc gacgtgagca tagattagaa ttggaaaaag
    15841 aaggatagac aaatgagtca aaaattactg gggattgatt taggtggtac caccattaaa
    15901 tttggaattt taacagcagc aggagaagtc caagaaaaat gggcaattga gactaatatt
    15961 ttagaaggag gcaaacatat tgtccctgat atcatcgctt ccatcaaaca tcgcttagac
    16021 ttgtatggtc taagcagtgc tgattttgtg ggaattggta tgggatcacc aggagcggtt
    16081 gaccgtgata ctaatacagt tactggggca ttcaacctta attggaaaga aacccaagaa
    16141 gtcggttccg ttgttgaaaa agaattgggc attccttttg ccattgacaa tgatgccaat
    16201 gtggctgccc ttggtgaacg ttgggtaggt gctggtgaaa ataacccaga tgtcgtcttc
    16261 atgacacttg gaacaggtgt cggtggaggc attattgctg atggtaactt gattcatggt
    16321 gttgcaggag caggtggtga aatcggccac atgattgttg agccagaaaa tggctttgct
    16381 tgtacttgtg gctcacacgg ctgtttggaa acagtagctt cagcaacagg agttgtcaaa
    16441 gtggcacgtt tattggcaga agcttatgaa ggggattcag ccatcaaagc agctattgac
    16501 aatggtgaag gtgttaccag caaagatatt tttatggcgg ctgaagcagg ggattccttt
    16561 gctgattctg ttgtggaaaa ggttggttac taccttggcc ttgcttcagc aaatatttcc
    16621 aatatcttga atccagactc agtggttatc ggtgggggtg tgtcggcagc aggagaattt
    16681 ctacgctcac gtattgaaaa atactttgtg accttcactt tcccacaagt gaggtattca
    16741 actaaaatta agattgcaga gcttggcaat gatgctggaa ttatcggtgc agctagcctt
    16801 gctcgacaat ttatcacaaa ataggagggg gatatgtctc caatcacact tatcttatgg
    16861 ttacttcttg tcggcattgt tggttattat acatggaact atttctcttt tcgaaaaatg
    16921 gcaaagcagg ttgataacga gacttttaaa gacgtgatgc gtcaaggtca attgattgat
    16981 ttacgagagc cagcggcttt tagagctaag catattttag gagctcgaaa ttttccagcg
    17041 caacaatttg atgctgctat caaaggtctt cgcaaggaca aacctgttct tatttacgaa
    17101 aatatgcgtc cccagtaccg tgtgcctgcg gtcaaaaaac ttaaaaaagc tggctttgaa
    17161 gacgtttatg tcttaaaaga cggtattgat tattgggatg gcaaagttaa acaaacaacc
    17221 tagttaagag taacgtggag ttaaggggaa taaaggtagt cactgtctca aaaaccttaa
    17281 atcctttttt tgctgtatcc agacttgctg aaagtctgaa aacatttaca attgattaaa
    17341 accagttttt taaaacattt tgtgttatac ttatctagtt aaaatatatt tacttagagg
    17401 aacaaatgac taacttaaga aacgatatcc gtaacgtagc gattattgcc cacgttgacc
    17461 acggaaaaac aacacttgta gatgaattat taaaacaatc ccatactctt gatgagcgta
    17521 aagagcttca agagcgtgcc atggattcca atgaccttga aaaagaacgt gggattacaa
    17581 tccttgcgaa aaatacggca gtagcctata acgatgttcg tattaacatc atggataccc
    17641 caggacacgc ggacttcggt ggtgaagttg aacgtatcat gaaaatggtt gacggggttg
    17701 ttcttgttgt ggatgcctac gaaggaacaa tgccccagac gcgtttcgta ttgaaaaaag
    17761 cacttgagca aaaccttatc ccgatcgttg tggtgaacaa gattgacaaa ccttcagctc
    17821 gtccagcaga agttgtagat gaagtgcttg aattattcat cgaacttggt gccgatgatg
    17881 agcaattgga attcccagtt gtttacgcat cagctattaa tggaacatca tcattatcag
    17941 atgaccctgc tgaccaagag catactatgg caccgatctt tgatacgatt attgatcata
    18001 ttccagcgcc agttgataat tcagatgagc ctttgcaatt ccaagtgtca cttttggact
    18061 acaacgattt cgtaggtcgt atcggtatcg gtcgtgtttt ccgtggtact gttaaagtgg
    18121 gtgaccaagt aactctttca aaacttgatg gtaccactaa aaacttccgt gttacaaaac
    18181 tgtttggttt cttcggtttg gaacgtcgtg aaattcaaga agctaaagca ggtgacttga
    18241 ttgctgtttc aggtatggaa gatatctttg ttggagaaac cattacacca actgactgtg
    18301 tggaagctct gccaattctt cgtattgatg agccaacact tcagatgact ttcttggtca
    18361 ataactctcc ttttgcaggt cgtgaaggta aatggatcac gtcacgtaag gttgaagaac
    18421 gtcttttagc agaattgcaa acagacgtgt cacttcgtgt tgacccaaca gattcgccag
    18481 ataaatggac ggtttcaggg cgtggagaat tgcatttatc tatcctcatt gaaaccatgc
    18541 gccgtgaagg ctatgaactt caagtatcac gtccagaagt tatcatcaaa gaaattgatg
    18601 gtgtcaaatg tgaaccgttt gagcgtgttc aaattgatac accagaagaa tatcagggtg
    18661 caatcatcca gtctctttca gaacgtaaag gggatatgct tgatatgcag atggttggta
    18721 atggtcaaac gcgtttgatt ttcttgattc ctgcacgtgg tttgattggt tattcaacag
    18781 agtttctttc aatgacacgt ggatatggta tcatgaatca tacttttgat cagtatctac
    18841 cggttgttca aggagaaatc ggtggccgtc atcgtggtgc cttggtttct attgaaaatg
    18901 ggaaagcaac gacatattca atcatgcgta ttgaagaacg tggaactatc tttgtcaacc
    18961 caggtacaga agtttacgaa gggatgattg ttggtgaaaa ctcacgtgat aacgaccttg
    19021 gtgttaacat tacaacagct aagcaaatga caaatgtccg ttcagcgact aaagatcaaa
    19081 ctgcagttat caagacgcct cgtatcctaa cgcttgaaga atcattagag ttcttgaatg
    19141 acgacgaata catggaagta acgccagaat ctatccgctt gcgtaaacaa atcttgaata
    19201 aagctgcgcg tgataaagct aataaaaaga aaaaatcagc cgaatagctt ttgaagttaa
    19261 actaactaaa taaaatagca aaaagatatt taagaaaagt cattttttaa aggtcttttt
    19321 gcgtttacaa gttcctatgg aatataaacc aaaataaagg aggacggaat gttttacctt
    19381 attatggctg tctcgattat atcttattat ctatacatgg ctcctaaaag tgtccgtaac
    19441 acccttggga tgattggttt agttggcttg gttgctctgc ttattgtttt agcgggcttg
    19501 agttttatca aaatcatgca aacccctcct gaatttttta ttgggatggg aatggtggca
    19561 ttgggctatt ttgctttaaa agatgtgcgt aaaatgacca aaaaaccgag agttaaataa
    19621 aaatacttaa agattgatga ttttggtttt tatacgataa aaggactgat ttgttttaga
    19681 caatatcagt ccttttttaa tagttcattt tttctgaaag aaagctttta gccgattttc
    19741 attgagtgtg caactaaaaa aagatgcttg gtagtgacag ttaagacctc tgactagtat
    19801 tttatcttac tttacttaaa aaaacgtacc aataatctca caaatatggg catgatgttt
    19861 gtatattgga aacggattct aattctaaac tcaagaacgg tatttttaaa atgttgaaaa
    19921 agcatctaaa taaaggtaaa atagaaatca aatagaatgg atagtgtgag cttagatgaa
    19981 agtgataagt aattttcaaa ataaaaaaat attaatattg gggttagcca aatcgggcga
    20041 agcagcagca aaattattga ccaaacttgg tgctttagtg actgttaatg atagtaaacc
    20101 atttgaccaa aatccagcgg cacaagcctt gttggaagag gggattaagg tcatttgtgg
    20161 tagccaccca gtagaattat tagatgagga ctttgagtac atggttaaaa accctgggat
    20221 tccttatgat aatcctatgg ttaaacgcgc ccttgcaaag gaaattccca tcttgactga
    20281 agtagaattg gcttatttcg tatctgaagc gcctattatc gggattacag gatcaaacgg
    20341 gaagacaacc acaacgacaa tgattgccga tgttttgaat gctggcgggc aatctgcact
    20401 cttatctgga aacattggtt atcctgcttc aaaagttgtt caaaaagcaa ttgctggtga
    20461 tactttggtg atggaattgt cctcttttca attagtggga gtgaatgctt ttcgccctca
    20521 tattgctgtc atcactaatt taatgccgac tcacctggac tatcatggca gttttgagga
    20581 ttatgttgct gctaaatgga tgattcaagc tcagatgaca gaatcagact accttatttt
    20641 aaatgctaat caagagattt cagcaactct agctaagacc accaaagcaa cagtgattcc
    20701 tttttcaact caaaaagtgg ttgatggagc ttatctgaag gatggaatac tctattttaa
    20761 agaacaagcg attatagctg ccactgactt aggtgtccca ggtagccaca acattgaaaa
    20821 tgccctagca actattgcag ttgccaagtt atctggtatt gctgatgata ttattgccca
    20881 gtgcctttca cattttggag gcgttaaaca tcgtttgcaa cgggttggtc aaatcaaaga
    20941 tattaccttc tacaatgaca gtaagtcaac caatatttta gccactcaaa aggctttatc
    21001 aggttttgat aacagtcgct tgattttgat tgctggcggt ctagatcgtg gcaatgaatt
    21061 tgacgatttg gtgccagacc ttttaggact taagcagatg attattttgg gagaatccgc
    21121 agagcgtatg aagcgagctg ctaacaaagc agaggtctct tatcttgaag ctagaaatgt
    21181 ggcagaagca acagagcttg cttttaagct ggcccaaaca ggcgatacta tcttgcttag
    21241 cccagccaat gctagctggg atatgtatcc taattttgag gttcgcgggg atgaattttt
    21301 ggcaaccttt gattgtttaa gaggagatgc ctaatgccta agaagatttt atttacaggt
    21361 ggtggaactg taggtcatgt caccttgaac ctcattctca taccaaaatt tatcaaggac
    21421 ggttgggaag tacattatat tggtgataaa aatggcattg aacatacaga aattgaaaag
    21481 tcaggccttg acgtgacctt tcatgctatc gcgacaggca agcttagacg ctatttttca
    21541 tggcaaaatc tagctgatgt ttttaaggtt gcacttggcc tcctacagtc tctctttatt
    21601 gttgccaagc ttcgccctca agcccttttt tccaaaggtg gttttgtctc agtaccgcca
    21661 gttgtggctg ctaaattgct tggtaaacca gtctttattc atgaatcaga tcggtcaatg
    21721 ggactagcaa acaagattgc ctacaaattt gcaactacca tgtataccac ttttgagcag
    21781 gaagaccagt tgtctaaagt taaacacctt ggagcggtga caaaggtttt caaagatgcc
    21841 aaccaaatgc ctgaatcaac tcagttagag gcggtgaaag agtattttag tagagaccta
    21901 aaaaccctct tgtttattgg tggttcggca ggggcgcatg tgtttaatca gtttattagt
    21961 gatcatccag aattgaagca acgttataat atcatcaata ttacaggaga ccctcacctt
    22021 aatgaattga gttctcatct gtatcgagta gattatgtta ccgatctcta ccaacctttg
    22081 atggcgatgg ctgaccttgt agtgacaaga gggggctcta atacactttt tgagctactg
    22141 gcaatggcta agctacacct catcgttcct cttggtaaag aagctagccg tggcgatcag
    22201 ttagaaaatg ccacttattt tgagaagagg ggctacgcta aacaattaca ggaacctgat
    22261 ttaactttgc ataattttga tcaggcaatg gctgatttgt ttgaacatca ggctgattat
    22321 gaggctacta tgttggcaac taaggagatt cagtcaccgg acttctttta tgaccttttg
    22381 agagctgata ttagctccgc gattaaggag aagtaaatgg caaaagataa agagaaacaa
    22441 agtgatgaca agctcgtttt gacagagtgg caaaagcgta acattgaatt tttaaagaaa
    22501 aagaagcagc aagctgagga agaaaaaaaa ctcaaagaga aattattgag tgataaaaaa
    22561 gcgcagcagc aagctcaaaa tgcttctgaa gcagttgagc ttaaaactga tgagaaaact
    22621 gatagtcagg aaattgagtc agaaacgacg tcaaaaccta aaaaacccaa aaaagttaga
    22681 caacccaagg aaaaaagcgc gacacaaatc gcttttcaaa aatccttgcc tgttcttttg
    22741 ggggcgctct tacttatggc ggtgtctatt tttatgatca ctccttatag caaaaagaaa
    22801 gagttttctg taagaggaaa ccatcaaacg aaccttgacg aattaatcaa agctagcaaa
    22861 gtcaaagcat ctgactattg gttaacgttg ttaacttcgc ctggtcagta tgaacgaccg
    22921 attcttcgta ctattccatg ggtgaaatct gtacatctct cttaccaatt tcctaatcac
    22981 tttctattta acgttattga atttgaaatc atcgcttatg cacaagtcga aaacggtttt
    23041 cagcctattt tggagaatgg aaaacgtgtg gacaaggtca gggcatcaga actaccgaaa
    23101 tctttcttga ttcttaattt aaaagatgag aaagcgatcc aacagttagt taagcaatta
    23161 acgacattac ctaaaaaatt agtcaagaat atcaagtcag tgtctcttgc aaattccaaa
    23221 acgacagcgg atttactact tattgaaatg tatgacggta atgtagttag agtaccgcag
    23281 tcacaactca cattgaaact tccctattat caaaaattga aaaaaaacct tgaaaatgat
    23341 agtatagtgg atatggaagt tggaatttac actacaacac aggagattga aaatcaacct
    23401 gaagttcctc ttacgccaga acaaaacgca gctgataaag aaggagataa gcctggtgag
    23461 catcaggaac agacagacaa tgattcagaa acgccagcaa atcagagtag tcctcagcaa
    23521 gcaccaccat ccccagaaac ggtcctcgaa caggcccatg gctagctaat atctaagttg
    23581 aaaaagcaat gaaaacgtta gaaattcaac gattctaacc cataatgaat tgcctaaaaa
    23641 aaattaagtt tatataacaa aaaacgtaaa atgataacat tttacgtttt tttatggtat
    23701 aatattttct gaatgattct gttttttagc agtttttaga atagcaaaag tttggaaagt
    23761 aagtgaggtc aagtgaatgg ctagaaatgg cttttttact ggtttggaca ttggaacaag
    23821 ctcgataaaa gttttagtag cagaatttat ttctggtgag atgaacgtca ttggtgttag
    23881 taatgttcca agtaccggcg taaaagatgg cataataatc gatatagagg cagctgcgac
    23941 tgccatcaaa actgcggtag aacaagcaga agaaaaagca gggatgacaa ttgaaaaggt
    24001 taatgttggg ctaccggcaa accttcttca aattgaacca acacaaggaa tgattcctgt
    24061 cccaagtgag tctaaagaga taaaagatga ggatgttgat agcgttgtta aatcggcttt
    24121 aacaaaaagt atcacaccag aacgagaggt tatctcttta gttccagaag agttcattgt
    24181 ggatggcttt cagggcattc gagatccacg tggtatgatg gggattagat tagagatgcg
    24241 cgggcttatt tatactggac caagcaccat tttacataat ctgcgtaaaa cggtagaaag
    24301 agcaggcatt aaagttgaaa acatcattat ttctccgtta gctatggcta aaaccatttt
    24361 aaacgaaggt gagcgcgagt ttggagctac tgtaattgat atgggaggtg gacagacaac
    24421 tgtcgcttct atgcgagcac aagaattgca gtataccaat atatatgctg aaggcggcga
    24481 atacattact aaagatatat caaaagtatt aaaaacgtct ttggctattg cagaagcact
    24541 taagtttaat tttggtcaag cggagatatc agaagctagt ataactgaaa cagtaaaagt
    24601 tgatgtggta ggtagtgaag agcctgttga ggtaactgaa cgttatttat ctgaaattat
    24661 ttcagcgcgt attcgtcata ttttagatcg tgtgaagcaa gatttggaaa gaggtcgttt
    24721 actagactta ccaggaggca ttgttttgat tggtggcggt gcaatcatgc ctggagtggt
    24781 agaaattgca caagaaatct ttggagtaac tgtaaagctc catgttccaa atcaagtcgg
    24841 tattagaaat ccaatgtttt caaacgttat cagtttggta gaatatgttg gtatgatgtc
    24901 tgaagtagac gttttagcac aaactgcagt ttcaggagaa gaacttttgc gacgcaagcc
    24961 tatcgatttc agtggtcaag aatcttattt accagattat gatgattcaa gaagaccaga
    25021 atcgaccatt ggctatgaac aacaagcgtc acaaacagca tatgattcac aagttccgag
    25081 tgatcctaaa caaaaaattt cagaacgtgt tcgtggcata tttgggagta tgtttgatta
    25141 aaagtaataa agtgaggaga taaaatggcg ttttcatttg atactgcatc aattcaaggt
    25201 gcaattataa aagtaattgg agtcggcgga ggtggcggaa atgccattaa tcgtatgatt
    25261 gatgaaggtg ttgctggtgt cgagttcatc gcagcaaata cagacattca ggcattaagc
    25321 tcatcaaaag ctgaaacggt tattcaacta ggccctaaat taactcgtgg acttggtgct
    25381 ggaggacaac ctgaagtagg acgtaaagct gctgaagaaa gcgaagaaat tttaacagaa
    25441 gctcttacag gagcggatat ggtatttatt actgccggta tgggtggtgg ctctgggaca
    25501 ggggctgcac cggttattgc tcgtatcgct aagagtttgg gagccttgac agtagctgtt
    25561 gttactcgcc cgtttggttt tgaaggtaac aaacgtggta attttgctat tgaaggtatc
    25621 gaagaactcc gtgaacaagt tgatactttg ttaattattt caaataataa ccttcttgag
    25681 attgttgata aaaagacacc tttattagaa gcacttagtg aagctgataa tgttttacgt
    25741 cagggagttc aagggataac cgacttaatt actagtcctg gccttatcaa tctcgatttt
    25801 gccgacgtga aaacagttat ggcaaataaa gggaatgcct taatggggat tgggattggt
    25861 tctggagaag agcgcattgt tgaggcggcg cgtaaggcaa tctattcacc cctattagaa
    25921 acgactattg atggtgcaca agacgttatt gtgaacgtta caggaggtct cgacatgaca
    25981 ctcacagaag ctgaagaagc ctctgaaatt gttgggcaag ctgctggtca aggcgttaac
    26041 atttggttag gaacatctat tgatgatact atgaaagatg acatccgtgt gactgttgta
    26101 gcaactggag tgcgccaaga aaaagccgaa caagtttcag gttttcgtca gcctaggact
    26161 tttacccaaa ccaacgcgca gcaagtagcg ggtgcacaat atgcatcaga tcaagcaaaa
    26221 cagtcggttc aaccagggtt tgatcgtcgc tcaaattttg attttgacat gggggagtct
    26281 cgcgagatac caagtgcaca aaaggtaatt tctaatcata atcaaaatca aggttctgct
    26341 tttggaaatt gggatttgag acgtgataat atttctcgtc caacagaagg tgaattggat
    26401 aaccatctta atatgtcaac gttctcagct aacgatgaca gtgatgatga attagaaacg
    26461 cctccattct ttaaaaaccg ttaataatgg atttactgac aaataaaaag aaaatttttg
    26521 agactatccg cttatctaca gaggcagcaa ataggactaa tgatagtgtt tcagttattg
    26581 ctgtaacaaa atatgtggat agtacaattg caggtcagct tatcgaagca ggaattgagc
    26641 acattgccga aaaccgtgtt gataaatttc ttgaaaagta tgatgcgtta aagtatatgc
    26701 cagtaaagtg gcatttaatc ggtaccttac aacgtcgtaa agtcaaggaa gttatcaatt
    26761 atgttgatta ttttcacgct ctagattctg tgagattagc tttggaaatc aacaagagag
    26821 ctgaccatcc tgtgaagtgt tttctacaag ttaatatttc taaagaagag agtaaacatg
    26881 gttttaacat ttctgagatt gatgaagcga ttgaagaaat aggtaagatg gagaagatac
    26941 agttagttgg tttaatgact atggcaccag caaatgccag taaagaaagt attataacta
    27001 tttttcgaca agcaaatcaa ttaagaaaaa acttgcagtt aaaaaaaaga aagaatatgc
    27061 cttttacaga attgagcatg ggcatgagta acgattatcc aattgctatt caagaaggct
    27121 caacttttat tcggattggt agagctttct ttcactaatg gagaataaga tggcttttaa
    27181 agatacattt aacaagatga tttcttattt tgacacggat gaggttaacg aagttgaaga
    27241 agatgttgca gcatcaactg ataacgttat tccaagatca caacaatcag tcagagcaag
    27301 tagtcatcca aaacaagaac ctagaaacaa tcacgtacaa caagatcatc aggcgagatc
    27361 ccaagaacag acaaggtcac aaatgtatcc aaaacatggt acttctgaac gctattatca
    27421 gcagtctcag ccaaaagaag gccatgaaat ggttgacaga agaaaacgga tgagcacttc
    27481 tggtattgca aatcgccgtg agcagtatca acaatcaact tgttcagatc agacaactat
    27541 tgccttaaaa tatcctcgta aatatgagga tgctcaagaa attgtggatc ttttaatagt
    27601 taatgaatgc gttttgattg attttcagtt tatgctagat gctcaggcta gacggtgttt
    27661 agattttatt gatggtgcta gtaaagtgct ctatggtagc ttacaaaagg tcggctcttc
    27721 aatgtactta ctggctccgt caaatgtatc cgtcaatata gaagaaatga ctatcccaca
    27781 tactacacaa gatattggct ttgattttga tatgaaaagg cggtaaataa atgatattaa
    27841 tactatctat tcttcttcgt ctgatcaaag tttacactta tttattgatt gcttacgcat
    27901 taatgtcatg gtttcctggg gcatatgatt caaaaattgg gcgtttgatt agtggtatcg
    27961 ttgaaccaat tttaaaacct tttagagcat ttaatttaca atttgccggt cttgacttca
    28021 ctatttttgt cgtcattatt agtttgaatt ttttagctca agttttggtc cgtgtgttta
    28081 tttaatggtt agtcatagta agatttatca gcattttcac caagaagaat atccttttat
    28141 tgatagaatg tctgatatga ttaatagagt tggagattac tatcttttag aagttactga
    28201 gtttttaaat cctagagaag tgatgatttt aaaaagtttg attgctttaa cagatctaaa
    28261 aatgttcgta tcaacagatt actacccaag cgaatatggt cgtgtcatta ttgcacctgg
    28321 ttactatgac ttagaacaaa gtgattttca aatagcttta gtagagataa gttatcaggc
    28381 aaagtttaat cagttgacac atagtcaaat tttaggaact ttaattaatg aattaggagt
    28441 aaagcgaaat ttatttggag atgtttttgt tgaaatggga tatgcccagc tcatgattaa
    28501 gcgggagtta ttggattatt ttttaggaac aattactaaa atagctaaaa ctagtgtgaa
    28561 attaagagaa gttaactttg atcagttaat taggtctatt gataacagcc agaccctgga
    28621 tattctagtt tctagttttc gattagatgg tgtagttgct actatcttaa aaaaatctcg
    28681 aacgcaagtt atagcattaa ttgaagcaaa taagattaag gtaaactatc gactcgctaa
    28741 taaagcttca gataatctag tcatagggga tatggtgagt atcagaggtc acgggcgttt
    28801 tactctttta gcagataatg gagtgaccaa acatggcaaa caaaaaataa cactaagtaa
    28861 aatgatacat aaataaggag atacaatggc acttacaacg ctagaaatta aagataaaac
    28921 ttttaagaca aaattccgag gatactgtga agaagaagtc aatgaattcc ttgatattgt
    28981 tgttgatgat tacgaagctc ttgtacgtaa aaatcgtgat aacgaagcaa gaattaaaga
    29041 tcttgaagaa aaattatctt actttgatga aatgaaagag tcacttagcc agtctgtaat
    29101 cttagctcaa gaaacagctg aaaaagtgaa agcaacggct aatgcggaag caacaaactt
    29161 ggttagtaaa gcgacttatg atgctcagca tttattagat gaatctaaag ctaaagccaa
    29221 tcaaatgttg cgtgatgcaa ctgatgaggc taagcgagtt gcgattgaaa cagaagaact
    29281 aaagcgtcaa acacgtgtgt tccaccaacg tttaatctca tctattgagt cacaattaag
    29341 cttatcaaat tcacctgaat gggatgaact gctacaaccg actgcaatct atcttcaaaa
    29401 ttctgacgat gcttttaagg aagtcgtgaa gaccgttctt aatgaagaca ttcctgaatc
    29461 tgatgatagt gcctcttttg atgcaacacg tcagtttact ccagaagagt tagaagaatt
    29521 gcaacgtcgt gttgatgaaa gcaataagga gttagaggct taccaacttg actctcaatc
    29581 tgattctacg actgagccag aggtaaatct cagtgaaaca caaacgttta aattaaatat
    29641 ctaaataaag aaaaacaaga tgatttgctt ataatcacag caagcgggag atgatgagag
    29701 tctcgtattc cctaagtatt atcattatca ttttctaaaa ttttcgctga actacagtaa
    29761 gcgaggaggt ttgttcacct tacgagcaaa gagggagcgg taagtcttat gatatattat
    29821 cgctctgaac taaggtggta ccacgagctt tcgtcctttt tgagacgagg cttttttcgt
    29881 tatagtcgca agtttgttta aaaggagttt gaaataatga aattaaaaga gacacttaat
    29941 ctaggaaaaa cagcctttcc aatgcgcgca ggtcttccta ataaagagcc acaatggcaa
    30001 gcagcttggg aacaagcaga gctttacaaa aaacgtcaag aattgaatgc aggtaagcct
    30061 gcctttcatc ttcatgatgg acctccatac gctaacggca atattcacgt tgggcatgcc
    30121 cttaataaaa tttcaaaaga tatcattgtg cgttctaagt ccatgtctgg ctttcaagcg
    30181 ccttatgtac ctggttggga cacacacggc cttccaatcg aacaagtctt ggctaagcaa
    30241 ggaatcaaac gtaaggagat ggatttagca gaataccttg agatgtgtcg tcaatacgct
    30301 ttaagtcagg ttgataaaca gcgagatgat ttcaaacggt taggtgtttc ggcagactgg
    30361 gaaaatcctt atgtcacttt ggacccacag tttgaagctg atcaaattcg tgtttttgga
    30421 gcgatggctg aaaaaggtta catctatcgt ggtgctaaac ctgtttattg gtcatggtct
    30481 tcagaatcgg ccttggcaga agctgaaatt gaataccatg acatcgattc aacctcttta
    30541 tactacgcca ataaagttaa agatggtaaa ggtatccttg atactaatac gtatatcgtt
    30601 gtttggacaa caacaccatt tacggttaca gcatcacgcg gtttgactgt tggtcctgat
    30661 atggattatc ttgtggtgaa acctgctggt tcagaccgtc aatatgttgt ggcagaaggt
    30721 cttttggata gccttgctgg aaaatttggc tgggaatctt ttgaaacttt agctagccat
    30781 aaaggagctg atttagagta cattgtgaca gaacacccat gggatactga cgtagaagaa
    30841 ttggttatcc ttggtgacca tgttaccctt gagtcaggga cagggattgt ccacacagca
    30901 ccaggttttg gtgaggatga ctacaatgtt gggacaaaat ataaactgga agttgctgtg
    30961 actgttgatg aacgtggcct aatgacggaa aatgcaggtc cagatttcca cggacaattt
    31021 tataacaagg taacgccgat tgtcattgat aaacttggtg atcttttatt ggcacaagaa
    31081 gtgatcaatc actcttaccc atttgactgg cggacgaaaa aaccaatcat ctggcgtgct
    31141 gtaccgcaat ggtttgcttc tgtttctgat ttccgtcaag atattttgga tgaaattgaa
    31201 aaaacaacct ttcatccatc atggggtgaa acacgccttt acaatatgat tcgtgatcgt
    31261 ggcgactggg ttatttctcg tcaacgtgct tggggagttc cgcttcctat cttctatgcc
    31321 gaagatggca cagctattat gactaaagaa gtgacagatc atgtggctga tttgttccaa
    31381 gaaaatggat caatcatttg gtggcaaaaa gaagctaaag acctattacc agagggcttc
    31441 actcatccag ggtcaccaaa tggcgagttt acaaaagaaa ctgatatcat ggacgtttgg
    31501 tttgactctg gttcttcttg gaatggagtc atgaatacta aagaaaatct ttcctatcca
    31561 gcggatctct acttagaagg ctctgaccag tatcgtggtt ggttcaattc atccttaatt
    31621 acatctgtgg ctgttaatgg gcatgctcca tataaggcta ttttatcgca aggctttgtt
    31681 cttgatggta agggcgaaaa aatgtctaaa tcaaaaggca atatcatttc tcctaatgat
    31741 gtggctaaac aatatggtgc agatattctt cgactttggg tagcttctgt ggataccgat
    31801 aatgatgtcc gtgtttctat ggagatcctt ggtcaagtct ctgaaactta ccgtaaaatc
    31861 cgtaacaccc ttcgcttctt gattgctaat acatctgatt tcaaccctgc tacggataca
    31921 gtagcttatg cagatttagg agctgttgat aagtacatga caattgtctt taatcagttg
    31981 gtagcaacga ttactgacgc ttatgagcgt tatgacttta tggctattta caaagcagtg
    32041 gtgaactttg ttacggttga tttatcagcc ttctatcttg attttgctaa agacgttgtc
    32101 tatattgaag ctgccaatag cttagaacgt cgtcgcatgc agacagtctt ctatgatatc
    32161 ttggttaaga ttaccaagtt attgacgcca atcttgcctc acactactga agaaatctgg
    32221 tcttatctag agcatgagtc agaagcattt gttcaattgg cagaaatgcc tgtggcagaa
    32281 actttctcag ctcaagagga tattttagaa gcttggtctg ctttcatgac tttgcgtacc
    32341 caagctcaaa aagctttgga agaagcgcga aatgctaaga ttatcggtaa atcattggaa
    32401 gcccatttga ccatctacgc tagcgaagaa gtgaaaacct tattgactgc tttagacagc
    32461 gatattgctt tgcttttgat cgtgtctcaa ttaaccattg ctgacttggc agatgcgcct
    32521 gcggatgcag tggcatttga aggtattgcc tttatggtag aacatgccat aggtgaagtt
    32581 tgtgagcgtt cacgccgcat cgacccaact actcgcatgc gttcttacaa tgcatttgtc
    32641 tgtgatcaca gcgctaaaat cattgaagaa aatttcccag aagctgtggc tgaagggttt
    32701 gaagagagtg acaaataagt cttatcaaga cttgaagtga cactgagtaa gaagacaacc
    32761 atcgggtctt agggcctgat ggttttttag tagagctgat tacgtccatt agccagcttt
    32821 aacgagagac aaagtaggag aaatttagag tactttttta atttctttta agaaaattgc
    32881 tggcttcata taaggactat taagatagag ttcacctttc gctaataaaa aaacttagct
    32941 cttttagaag ggctaagttt tgtctgaata gctggctgct gtttatacag aagtttgaaa
    33001 tgaaatttca ataaggttat tcgtgtgaag tgtttttgat tgtccaggaa taacaatttt
    33061 aggatcaatt ttacgtttta aaaaaaattc gcgaatgata tcaagttcat cctccttgat
    33121 ggcacggtat ttatttgaaa taagtagttc gtaatgtttt ggtgcttggg taaaaatgac
    33181 atctgtattt cctgctgagt atacttcgac taaataggca tcggtgtttt ttagctgatt
    33241 tctgatgagt tctgggtgac tgctagtggt attgatgagt ctcataaatg tcctccttag
    33301 tattcctgta cctattatag cactaattta aagccttagt tagtagaatg atgctttttt
    33361 tgccattcct tgactcccca ttgatggctc tctcgtttta atttagcaat tgcctcgata
    33421 ggagaaaacc aacttaagtt attaaaatct tctaagggct tagaaacggc ttgaaatgcg
    33481 gttacttcat aaagataggc aggatggtag aagtgggtat cacgatggcg agagtagaaa
    33541 tattcatcag cctgaccgta atatgaacca atagtagcag aaaaaccaag ttcttcaatc
    33601 agttcacgct ctagggcctg aagctgacct tcacctgctt caatttcacc accaggtaaa
    33661 aaccaggaac cattaggagc ttgaacaaga ataatttttg tttgttcgtg attgggaatg
    33721 atagcataga caccgtaacg tgtgacatag tctttatgtg catttttatg tccaaatgtt
    33781 ggaatcatca ttttatttac ctttcctttt tcttcattat actaaatttt tgctaattat
    33841 tcttatcaat ttgtttagtt taataaaaaa ttgatagaaa aagagagagt ctccttatag
    33901 aaactctctt agctaacgtt aatctagaga ctcttcgtta agaaaagaat ctccctcttt
    33961 aacagcttta atcgtgatgt tttcactgtt tgtcatcagt gcacgtaact gtttttcagt
    34021 tgggtgttct aaataatagt ctgtaatggc atcttcgata taatcttgga tagtacgacg
    34081 gagcggccga gcgcccattt taggatcata gcctaaatct actaattttt ctttgacctt
    34141 ttgtgtcaca tcaagatgaa tcccattata gcctaagcgc tcattaacat cttccagcat
    34201 taaatcaacg atatgcagca aatgctcttt agataaagct ttgaattcaa taataccatc
    34261 gaaacgattc ataaactctg ggctgaagaa gttgcttaat tcaccaagga cagaacttgt
    34321 ccgtccttct ctagcagcac caaatccgac agaagcttcg cttttacctg ttccggcatt
    34381 agaggtcatg ataataatgg tgtccttgaa gctgactgtt cgcccttggc cgtctgttaa
    34441 gcgaccgtca tcaagaactt gtaagaacat gtgcatgacg tcaggatggg ctttttccac
    34501 ctcatctaag agaataaggg aataaggatt gcgacgaact tgttcggtta gctgtccagc
    34561 ttcttcatag ccgacataac ctggaggagc cccgactaat ttggcgacag cgtgtttttc
    34621 catgtattcg gacatgtcaa agcgaatcat attgtttgtc gaaccaaaga gttcaatggc
    34681 tagttgttta gacaattcag ttttaccaac cccagtcggt ccaacgaata agaaagaacc
    34741 aatagggcga tttggagttc ctaatcccac acggttacga cgaatagcct tagcgatttt
    34801 atcaacagca tcgtcttgcc cgatcacgtg tgctttcaga tcattggcta agtttacgag
    34861 ttgagactgt tccttttctt ttaagtcccc aactggaata ttagtttttt gctctacaat
    34921 agcttcaatg gttttttcag taatgatggg aatatcttgc tcatcgactt tttgagcctg
    34981 catttctttg tattttgtaa tttgatcgcg gaaataagct gcgcgttcat agtcttcgtc
    35041 tcgagtagct tgcgccttga gattctcagc ttcaataaga cgtttgtcaa tttctttagg
    35101 atcaacaaag ttgagggtta gattcatttt ggaaccagct tcgtccagaa ggtcaatagc
    35161 cttatcagga agaaaacggt cttgaatata gcggttagat aaatgagcgg cagcttcaat
    35221 agcggctggg ctatatttga catgatgata gtcttcgtat ttcggttgga taccttttaa
    35281 aatcgtgatg gtttcctcaa cagaaggttc atcaacttta acgggttgca tacgtcgttc
    35341 taaggcagcg tctttttcaa taatacggta ttcatttaat gtagtagcac caacgagttg
    35401 caactcacca cgggccaagg ctggttttaa aatattacca gcatccatat tgccgtctcc
    35461 tgcagaacca gcaccgacaa tctcatgaat ttcatcaata aagagaatca catccttgcg
    35521 attgcgaatt tcttccatta atttttgcat gcgctcttca aactgaccac ggatacctgt
    35581 tccctgaacg aggctaacca catcaagacg aatcacttgc ttgccttgga gtttttgagg
    35641 aacagtacca tcaataattt tttgagccaa accttctaca acagcagttt taccaactcc
    35701 aggctcacca attagcacag gattattttt agtacggcgg ttgaggatct cgataacgcg
    35761 tgtaatctct tcgtcacgac caataacagg atcaatatta ccatttcttg caatatctgt
    35821 gacattaatc ccaaactctt ctaataagcc ctttgcttgc tgatttggtg tttgtggctg
    35881 agcaggtcgt tgtccgttgt agttaccacc atagcgtcct ccgccatttc cattttgccc
    35941 tgcctgagta ggtggggtat ttggaagatt accaaaagct ctaaaattat tcaaatcacc
    36001 aaaaaagtca tcaaagaaag gacttgtgga tctatcttgt gctctatatc ctggggttag
    36061 gccatttaaa atagaattgg caggatcgga tttcatgatt tgataacaat tttgacagag
    36121 atcaacctgt ctttgttttc cattaacact tgtataaaga tgaatagtag attcgtttaa
    36181 attacaattt tgacacagca taatatacct ccgagggttt atctaaaagt caaagtcatt
    36241 tagtcaaaaa tggtcaaact ttatattcat attatagcac cacggcttgg gaaatcaagt
    36301 aaaaagttta tcgctttagc gttagctttt agagagtaac atgacagagt aactttatta
    36361 agtttccaat gagcaaatgt ttaaaatcat tgaaaagagt aaaaaaagta aaatatcaat
    36421 aaatatatta ctttccttgt tagtttccct tgttttgtgt taaaataaga gggataaaaa
    36481 acgaatagga gaaacgaaat ggaatcacat ttggtgagaa tcatcaatcg tttggagttg
    36541 atggtgacag atggcggtaa tctcaagcga aattttgaac gcgatggtgt tgttgttgca
    36601 gaagttgcct tttcaaatga tcctgaaaat ggacctgttt tcacattgcg tgatgttgaa
    36661 gcgcgtgagt catattcctt tgatagcatt gacttgattg caatggaaat ctacgattta
    36721 ttatattaag cttgacatag taaagagact tgcctgaggc aggttttttt attgtcaaaa
    36781 agtattgtat taatattcat cagatggtat aattatcttt attgtaatga acctaggact
    36841 tatgaggaga tataaatgga tttgtcattt ttgcccaaat actgggccta ctttaactac
    36901 ggtgtacttg tcaccattat gatttcagtc agcgttgtct tttttggaac ccttattggt
    36961 gtcttggtaa ccctgattaa gcgtagtcat gtgaagccgt tgacctgggt cgttaatctt
    37021 tacgtttgga tctttcgggg aacacctatg gtggttcaaa tcatgattgc ctttgcttgg
    37081 atgcatttta acaatatgcc tactattggt tttggggttt tagacttgga cttttcaaga
    37141 ctacttcctg gaattattat catttcattg aatagcggtg cttatatttc agaaattgtt
    37201 agagcaggta ttgaggctgt accaaaaggg caattagaag cagcttattc actaggtatt
    37261 cgtcctcaaa atgccatgcg ttatgtgatt ttgcctcagg cctttaaaaa tattttgcca
    37321 gccttaggaa atgaatttat taccattatt aaggatagtg cttttttaca aaccattgga
    37381 gtgatggaac tttggaatgg tgcccaatca gtggtaacgg ctacttattc tccaatttcc
    37441 cctttactgg tggctgcttt ttactactta atggtcacaa cagtgatggc acagttattg
    37501 gcagtcttag aacgtcacat ggcgcaagga ggtaatcatt gatgactgag gccattattt
    37561 caattaaaga cttgcacaag tattatgggc acaatgaagt gcttaaggga attgatttag
    37621 acattatgcc aggagaggtt gtggtgatta ttggcccgtc tggttctgga aaatcgacct
    37681 tacttcgaac catgaatctt ttggaagtac caactaaggg acagattagg tttgagggga
    37741 ttgatattac cgataagaag aacgatattt tcagcatgcg tgaaaaaatg gggatggttt
    37801 ttcagcagtt taacctcttt cccaatatga ctgttttaga aaatatcact ttatcgccaa
    37861 tcaaaactaa gggaatggct aaagcagagg ctgacaaaac agccttgagc ttgttggaca
    37921 aagttggatt atcagaaaaa gccaaggctt atcctgctag tctttctggt gggcaacagc
    37981 agcggattgc gattgcgcgt ggactggcta tggatccaga tgttttactc tttgatgaac
    38041 caacttcagc tctagaccca gaaatggtgg gtgaggtctt ggctgtcatg caagatttgg
    38101 ctaaatctgg gatgactatg gttattgtga ctcatgaaat gggctttgct aaagaagtcg
    38161 cagatcgtgt gatttttatg gatggtggcg ttattgtgga agaaggaagt cctaaccaac
    38221 tttttgatct gactaaagaa gaacgtacaa aagatttttt gagtagagta ttataaatct
    38281 gttctatgaa ttataaaagg aagtcctaaa tcatttagga cttcctttta taatttgcta
    38341 aggcatttta taggagatta cttttctttt tgttaaaata aaaagaaata gttacattat
    38401 ttgcatagaa gaaagggatc tgttatggtt tcagtgatgt ggtttcgtag agatttaagg
    38461 attgatgatc aaaaagcttt agctcatgct attgctagtc aatcgccaac cttatgtgtg
    38521 tttcaattta acaaggagca attaaacctg ctacatagtc ggaatcaatc tagttttatt
    38581 actagcgttt tggcttttag aaagttatta aaatgtgaaa gtattgactt atatctaatg
    38641 tatggagact taatgacctg ttttgagcaa ttattgacac aattaaaaga ctggacagat
    38701 gtgtatttta actatgatga atctggatat ggtcgtttac gtgatcaaaa agcagcacaa
    38761 tttttcaaaa aaaatgggat tgctgttcat acctaccaag atcactattt acatggtagt
    38821 caagagatca ttaaccaatc aggtcagcct tataaagttt ttacgcccta ctataggatt
    38881 tggcaaaatt atccgaaaga gactcctatt aaagtagaac tatcgcaagg caggtggtta
    38941 aatcttgaga caccagatga tgttttaagg actgttgaga gctttaaaga tgaaaaatat
    39001 caggatgttg caacttttga tgaagcgtca aaacaactaa atcgatttat tcaagaccaa
    39061 ttagcagctt atcacgctaa tcgagatttt ccagcgcagc tagggacgag tcggttgtct
    39121 ccctttttaa gaataggagc tattggcatt cgcacggtat atcacgctgt gcgtcaagca
    39181 ccaaatagtc tagggcaagc tactttttta aaagaattag cttggagaga cttttacaat
    39241 atggtctatg tggcttatcc tgatcagaaa actcagccta tccaaaaagc cttttctcaa
    39301 atcgaatggg ttaataatcc tgactggttt cagttgtgga aagaaggtaa aacgggttac
    39361 cctattgttg atgctgccat gcttcagtta caaaagacag gatggatgca taatcgttta
    39421 cgaatgatag tagcttcttt tttaactaaa gatcttttat gtgattggcg tttaggagag
    39481 cagtattttc aacaacaact aattgattat gatgctgcca gtaatatcgg aggatggcag
    39541 tgggctgctt ccactggaac agatgctgtt ccttatttta gaatatttaa tcctgttact
    39601 caaggtaagc gttttgatcc caaaggcgag tttattaaag cgtatttgcc tcaactagaa
    39661 cacgttccag agaagtatct ccacgaaccg tggaagatgc caaaatacct tcaagagagt
    39721 gtatcctgta ttattggcac agactatcct caaccaattg tcgatcatgc taaacaacgt
    39781 gaacaagcta ttgcaaagta tgagtgggca aaagaaaaag caaaaataga atgactgttc
    39841 tcattataag agtttaaaca aaaagttatc atgaattaag attatttaat ttaaaaaata
    39901 agttgacttt atttagatta tttgatacta ttcagttgtc tcccttagag aaagcgattt
    39961 cttgttttgg agctgatttt taccatttgg tcttagaata ggatgagtaa tatgacttac
    40021 aacgaggtat atcaagaatg gttgcacaat aatgatctta gtgatgatat taaagcagat
    40081 ttagcagcca taaaagacaa tgaggctgag attcaagatc gtttttacaa aacacttgaa
    40141 tttggaacag cagggctaag aggaaaactt ggagcaggca ccaatcgcat gaatacctat
    40201 atggttggta aggcagcaca agctttagct aatactatta ttgatcatgg acctgaagcg
    40261 gttaaaaagg gcattgcggt tagttatgat gttcgctatc aatctagaac atttgcagaa
    40321 ttaacatgct ctattatggc agctaacggt attaaggcgt acctttataa agggattcgg
    40381 ccaacaccaa tgtgttcgta cgctattcgt gctttgggat gtatctctgg tgttatgatt
    40441 acagctagtc acaatcccca agcttacaat ggttataaag cttattggca agaaggttct
    40501 caaattttgg atgacattgc ggatcaaatt gcacaacaca tggctgcact aactcagtat
    40561 gaggaaatca aacaaatgcc ttttgaaaag gctctggaca gtggacttgt tacttatatt
    40621 gatgagagta ttgaagaagc atataaaaaa gaagtgcttg gtttaacgat taatgatact
    40681 gacattgata agtcagtccg agtagtctat acccctttaa atggtgttgg gaatctacca
    40741 gtgcgtgagg ttttaagacg ccgcggtttt gaaaatgttt atgtggtacc tgagcaggaa
    40801 atgcccgatc ctgattttac aacagttggc tatcctaatc ccgaagtccc taaaactttt
    40861 gcatattcag aaaaattagg taaggcagtt gatgctgata ttttgattgc aactgatcca
    40921 gattgtgacc gtgtggcttt agaagttaaa aatgcagtcg gtgactatgt ttttctcaat
    40981 ggcaataaaa tcggagcgct tttatcttac tatatctttt cacaacgatt tgacttaggc
    41041 aatttaccgg ctaatcctgt tttagtgaaa tccattgtaa caggagactt gtcacgggct
    41101 attgctagcc attatggtat tgaaaccgtt gaaacattga ctggttttaa gaatatttgt
    41161 ggcaaggcaa acgaatatga tgtgacgaaa caaaaaaact acctgtttgg ttatgaagaa
    41221 agtattggtt tttgctatgg cacttttgtt cgtgacaaag acgctgttag cgcctctatg
    41281 atgattgtag aaatggcagc ttactacaag aaaaaagggc agaatttact agatgttttg
    41341 cagactattt atgcgacatt tgggtattat aatgaacgtc aaattgcctt ggagcttgag
    41401 gggatagaag gacaaaaacg cattgcgagg ataatggaag atttcagaca gacaccaata
    41461 gctagtgttg cagagatggc gttagacaag acaattgatt ttattgatgg ctatcaagat
    41521 tttccaaagc aaaactgtct caaattttat ttggatgatg gttcttggta tgcacttaga
    41581 ccatcaggaa cagagcctaa aataaaattt tatttatata ctattggcca aacacaagaa
    41641 aatagtgcaa caaaattaga tgctattgaa gcagcgtgtc gcacaaaaat aaatcaagtt
    41701 aattaaacat caaaaatgga taacactttt acactatttt tccatataaa caactacaaa
    41761 aggaggtgag acttttaggc accttctttt tcttgtaggt gccactaatc aatttaatcg
    41821 actcaaacca atactaaaaa ataagtaaga gtataaagaa caggcatcta tttttttgtt
    41881 ataatagaaa aatgaaatta aagggagatg tcaatgacag aactaattga tggtaaagcc
    41941 ttagctcaaa agatgcaaca agagttagca gctaaagtca acaatctaaa acaaaaaaaa
    42001 ggaattgtac caggcttagc cgttattctt gtaggtgatg atcctgctag tcaggtgtat
    42061 gtccgtaata aagagcgtgc agctcttact gtaggtttta aaagtgagac cgttagatta
    42121 tcagaattca tttgtcaaga agagcttatt gcagtaatcg aacgttacaa tgcagataac
    42181 actattcatg gtattttagt gcagctaccc ctgccaaatc atattaatga taaaaaaatt
    42241 attctcgcca ttgatcccaa aaaagatgtg gacggttttc acccgatgaa tacaggtcac
    42301 ctttggtcag gacgtccctt aatggttcct tgtactccat cagggattat ggaattgctt
    42361 cgagaatata atgttaacct tgaaggtaaa catgccgtca ttattggcag atcgaatatc
    42421 gttggaaaac caatggcaca gctcttacta gacaaaaatg caacagtcac gttgacacat
    42481 tcaagaacac gtcaattaga agaagtatgt cgctgtgcag atgtgttgat tgtggcaatc
    42541 ggacaaggtc atttcataac aaaacaatat ataaaagatg gtgcgatagt gattgatgtg
    42601 ggaatgaacc gtgatgataa tggcaagcta attggagatg tggcctttga tgaggtggca
    42661 gaagttgcag cgaaaatcac ccctgtacca ggaggtgtcg gtcctatgac gattgctatg
    42721 ttgctagagc aaacttatca atctgccctc cgtagtactc ataaatagtt ataattagac
    42781 aattcaacat aagagaatat aagaagtcag caatggcttc cttttttgtt tttctgctca
    42841 ctatttatcc agaaaaatgg tataattgca ttagtatgca aagggactat gtcaggtcca
    42901 actaagtgaa aggtggcttg atggcagatt atttaaccgt cactcatttg acgaaatatt
    42961 taaaattaaa atttgaccgt gacccttatt tggaacgggt ttatctgact ggtcaagtgt
    43021 ccaattttcg aaaacgacca actcatcaat atttttcctt aaaagatgaa agtgctgtga
    43081 ttcaagccac catgtgggca ggagtctata aaaaactagg attcgaccta gaagaaggca
    43141 tgaaaattaa tgtaattggg cgagtccaac tttatgaacc tagtggctct tactctatcg
    43201 tgattgaaaa ggcagagcca gatggtatag gtgccttggc tttgcagttt gaacaattaa
    43261 agaaaaaatt aacggcagaa ggttattttg agcaaaaaca caagcagccc ttgccacagt
    43321 ttgtatcaaa aattggggtc attacaagtc ctagtggtgc tgtgattcga gatattatca
    43381 caaccgtttc acggcgtttt ccaggggttg agattttatt attcccaact aaagtacaag
    43441 gtgatggagc cgcccaagaa gtagtggcta atattcgaag agccaatcaa agagaggatt
    43501 tggacttgct cattgtcggc cgtggaggtg gctcgataga agacctttgg gccttcaacg
    43561 aggaaatagt ggttcaggct atctttgaat cgcaacttcc agtgatttcg agtgtgggtc
    43621 atgaaacaga cacgacttta gcggattttg tggcagatcg cagagcagcc acaccaacag
    43681 cagcagcaga gttagcaacg cctattacaa aaacagatct tatgtcttgg atagtagagc
    43741 ggcaaaatcg ttcctatcag gcctgcttga ggcggattaa gcaacggcaa gagtgggtgg
    43801 ataaactctc gcaatctgtt atttttaggc aaccagaacg attatatgat gcctacctgc
    43861 aaaaaattga tcgccttagc atgaccctga tgaatacgat gaaagatcgt ctgagttccg
    43921 ccaaagaaaa caaggttcag ttggaccatt ccttggccaa tagtcaatta cagacaaaaa
    43981 tcgagcgata ccaagaccgt gttgctaccg ctaagcgttt actgatggct aatatggcta
    44041 gtcaatatga tagtcagctg gctcgctttg aaaaagcaca ggatgctttg ctatcactgg
    44101 atgccagccg aattatcgcc cgtggttatg caatgattga aaaaaatcaa gcactggtag
    44161 cctctgtaag tcagataaca aagggtgatc agttaaccat taagatgcgt gatggacaat
    44221 tagatgtaga ggtaaaagat gtcaaaaacg aaaacatttg aagaaaattt acaagattta
    44281 gagactattg tgaacaaact tgaaaatggg gatgtccctt tggaagaagc tatttcagaa
    44341 tttcaaaaag gaatgcttct ctcaaaagaa cttcaaaaga ccttacaagc ggcagaaaaa
    44401 acactcgtca aagtgatgca agctgatggc acagaagtag atatggatga ttaatggaca
    44461 aattagctag aattgacgaa gccattcgtc gctactataa gacgactagt aacggtgtat
    44521 ctgaagagtt gattgatgct atcttgtatt ctgttgacag tggtggcaag cgcattcgcc
    44581 cacttatctt attagagatg attgagggat ttggggtatc gttacagaat gctcattttg
    44641 atttggctgc agcccttgaa atgattcata caggaagtct gattcacgat gatttgccag
    44701 ccatggataa tgatgattac cgacgtggta gactgaccaa tcacaaacaa tttggagagg
    44761 ctactgctat tttggcggga gatagcttat ttttagaccc ctttgggtta atagctcaag
    44821 ctgaattaaa tagcgaggtt aaagtagcct taatccaaga actttcccta gcttcaggaa
    44881 cttttggcat ggttggtggt cagatgctag atatgaaagg ggaaaatcaa gctttgagtc
    44941 tccctcagtt gtcactgatt cacctcaata aaaccggaaa attattaact tttcctttta
    45001 aagcagcagc tcttgtaaca gaacaggcta ttactgttcg tcaacaacta gagcaggcgg
    45061 gaatgctcat cgggcacgcc tttcagatta gggatgatat tttagacgtg acagctagct
    45121 ttgaagatct tggaaaaacg cctaaaaagg atttatttgc agaaaaagct acttacccaa
    45181 gtttattggg gttagaagcc tcctaccaac tgttgacaga gagtttagat caggctttga
    45241 cgatttttca gacactcgaa agcgatgtag gctttaagcc tcaaataatt acaaaactga
    45301 tagaagggtt acgacttaat gcctaaagaa agagtagatg tgttagccta taagcaagga
    45361 ttatttgaga ccagagagca agctaagcgt ggtgttatgg caggcttagt ggtctctgtg
    45421 attaatggcc aacgctatga caagccaggt gacaaaattg acgatgggac tgagttaaaa
    45481 cttaaaggtg aaaaactcaa atacgtcagt cgtggtggat taaaactgga aaaggggctg
    45541 cacgtttttg gtgtatcagt tgctaatcaa attgggattg atattggcgc ttcaacaggt
    45601 ggctttaccg atgttatgtt acaagatggg gccaaactag tctatgccgt cgacgttggg
    45661 actaaccagt tggtatggaa actcagacaa gatccacgag taagaagtat ggaacagtat
    45721 aactttcgtt atgctcagcc agaggacttt aatgagggac agcctgtatt ggccagtatt
    45781 gatgtgtcct ttatttctct cagcttgatt ttgccagccc tgcataacgt tttgtcagat
    45841 cagggacagg ttattgctct cattaaaccg caatttgaag ctgggcgcga gcagattggt
    45901 aaaaaaggca tcgtcaagga caaacagatt catgaaaaag tgattcaaaa ggtcatggat
    45961 tttgcctcag gttacggatt tacagttaag ggacttgatt tttcccctat tcagggtggt
    46021 catggcaata tcgaattttt agctcatctt gctaagtcac agacacctga aacgttagcc
    46081 ccgcatttga ttcaggaagt tgttgccaag gcacataagg agtttgagaa acatgaaaaa
    46141 gagtgaacga cttgaattaa tcaaaaaaat ggttttgacg catccaattg agacgcaaca
    46201 tgacctctta agattattgg cagagcatgg gttagaatta acccaggcta ctatttcaag
    46261 agacatgaat gaaattggta tagtaaagat tccttctggc agtggacgtt acatctatgg
    46321 tctttctcaa gatagtggaa aaaagatcgt tcaaggacct agatcgataa agagcactat
    46381 tttagctgta tctgacaaaa caaaaggttt agaacaacac ctctatttaa aagtcgtacc
    46441 tggcaatagt aaattaatca aacgttattt attagcagat ttttcaaaag ctatttttag
    46501 tcttattgct gatgatgata gtttattgtt aattgcaaag tctccttcag aagcagatat
    46561 gatacgccaa gaaattttgc tttggatgca gggtataacc tagaaaaaaa taggggagaa
    46621 agtgatgtta ttagaaattt ctattaaaaa ttttgctatt attgatgaaa tttccctaaa
    46681 ttttgaaaat ggtatgacgg ttttgacggg tgaaactgga gcaggaaagt ctattatcat
    46741 tgatgctatg aatatgatgc ttggtgcgcg tgctagtaca gaagtgattc gtcgtggagc
    46801 taataaagca gaaattgagg ggttcttttc ggtagatgct accccagaac tggttgcgtg
    46861 tctggaatca tcaggcattg ctatggaaga agaactgatt attcgccgag atatctttgc
    46921 caatggcaga agcgtgagcc gcattaatgg tcagatggtt aacctagcca ccttaaaaca
    46981 ggtcggacag tttttggtag atattcatgg gcaacatgac caagaagaat taatgcgacc
    47041 acagctccac cagcaaattt tggacgcttt tggtgataag gcttttgagc aattgaaaga
    47101 gaactatcag cttatttttg atcgttataa aagcttgcgt cgccaggtta ttgacaaaca
    47161 aaaaaatgaa aaggaacaca aagatcgtat tgatatgttg gcctttcaga tagcagaaat
    47221 tgaagctgct gccttgagtc gaggtgagga cgaccggtta aatcaagaac gtgatcgctt
    47281 aatgaaccac aaacaaattg ctgataccct gaccaatgcc tacgtcatgc tagataatga
    47341 cgatttttca agtctatcca atattcgctc tagcatgaat gacttactat caattgagca
    47401 gtttgattca gagtacaaag ggatgtcgac ttcgatttct gaagcctatt atattcttga
    47461 agaagtgagc aagcaattat cagataccat tgaccaactg gattttgatg gtgggcgatt
    47521 acaggaaatt gaatttcgct tggatacact caatagctta acccgtaaat acggtggtaa
    47581 tgtgaatgat gtgcttgact actacgataa tattatcaaa gaataccagc tattaacagg
    47641 agacgacttg tcttcagggg atctagaatc agaattgaaa gccttagaaa aacaattggt
    47701 tgctgcagcc actaacttga gtgaacgccg ccatcaattg gcagagcaat tagaagctga
    47761 gattaaagct gaacttaagg aactttatat ggaaaaagca gatttcaaag ttcactttac
    47821 cacatcaaaa ttcaatcgtg atggtaatga aagcttggaa ttttacatct ctactaatcc
    47881 aggcgaagga tttaagcccc ttgtcaaggt ggcttctgga ggagagttat cacgccttat
    47941 gttagccatc aaggctgcta tttctagaaa agaagataag acgagtattg tttttgatga
    48001 agtagataca ggtgtttctg gtcgcgttgc gcaagccatt gcccaaaaaa tttataaaat
    48061 tgggcgacat gggcaagttc tcgctatttc acacttacct caagttattg ctattgctga
    48121 ttatcagtat tttatttcta aagaaagtaa agaagagtcc accgtttcta aggtaaggtt
    48181 attaactcct gaagaacgtg tggaagaaat tgctagcatg attgcaggaa cagatatgac
    48241 acaagctgct cttacgcagg ctcgtgaact acttgccaaa cattaagatt gtgtttggta
    48301 tttgatattt tcataattag atgacattga ttttttaggt gctaaaataa tcattgtgct
    48361 agaccatttt tcctctgtta atctggcttg gtttgccttg acaaacctct gagaaatagt
    48421 agaatagata agagaatttt aagctgaggg gatagaaatg actagtaaaa aagcgtgttt
    48481 atcaagcatc attgtgttag caagtttaac gtgtggaaat gatactgtta gtgccaatca
    48541 tctctcagca actggagata agtttgatga ttgctcaaca cttgttgaaa aagatgtggc
    48601 ccctaaagat gaacttgaga tgttagcatg gtcctcgtct caaacaactg atgatgctga
    48661 cagagactat gaagattttc tcgatgatga ttcttttatt tctcaaaatg aaactgataa
    48721 gatgtttgag aatttaactg atgataggtt attagatgaa ttagatgaat tagatgaatt
    48781 agatgaatta gatgaagaag atacaattga gccagagcaa aatgtaataa tgcctagtga
    48841 cgatgagcta tttgatttaa ctgatgctgt tgagacacgc cttactgttt ctagtgctcc
    48901 ccatttagag gctgaattgc cgaaaccaca tttgaggagc ctatcagata cagcactgcg
    48961 gtctggtgaa attagaggac atttagataa caaactggac gctttgtctg taacagctac
    49021 aaagttagca ttaacgatgg ctcaaaaatt tgatttgaca acgcatgtct attctatagg
    49081 tgaaagcttt agtgaagtat tagctgctca ttatgaagac agaaaagcag aatcagcttt
    49141 ttctaagaaa aagagatttc accttcctat tgctactcca gatgttgtta tagaggagtt
    49201 aaggcgccta gtctcttcta ttggaagttc aaaagaagat gtttcagttc cttataatcg
    49261 gaagctaggt atggcagttg caaaaagaaa aatagccctg ccacaaacgg gagaggagtt
    49321 ctcttattat ccagctttac ttggtttaat gatattagga ttaacgccga ttatgatacc
    49381 aaagaagata aataattagg taataagtta ttgtagttgt aaaagacggg gcaaatgttc
    49441 aagcgtgaaa aggttgactt ttgcctcgtt ttgtatctcg ttattgctaa ggcattttct
    49501 tcgagcagct agtgctcgcc atacctatct accgagtgat tattcaatag cactaccagt
    49561 tttgctttat ttagtgagga gtgttagaat aaagaggtga gaaactagtt tagaatggaa
    49621 aaatacatgg gaactataaa aattgttaca gattcatcaa taactattga accagaatta
    49681 attaaagctt tagatattac tgtagtgcct ttatctgtca tgattgatag caaattatat
    49741 tctgataatg atttaaaaga agaaggtcat tttttgagtc ttatgaaggc tagcaaatcc
    49801 cttcccaaaa ccagtcagcc accggttggt ctctttgctg aaacttacga aaatttggtc
    49861 aaaaaaggag taactgacat tgttgctatt cacctctcac cagctttatc aggtactatt
    49921 gaagcttccc gtcagggggc tgaaatcgct gaggcccctg ttacagtttt agattcagga
    49981 tttactgatc aagccatgaa atttcaggtc gttgaggcag caaagatggc aaaagcgggt
    50041 gctagtttga atgagatttt ggctgctgtt caagctatca aatcaaaaac agagctctac
    50101 attggtgtat ctaccttaga aaatttagta aaaggaggtc gtattggtcg tgtgacgggt
    50161 gttttgagct cgctacttaa tgtcaaagtt gttatggcac ttaaaaatga tgagttaaaa
    50221 acgcttgtca aaggtcgagg taataaaaca tttacgaaat ggttagatag ttacctcgct
    50281 aaaaatagtc atcgcccgat tgcagagata gctatttctt atgctggtga agctagcctg
    50341 gcattaacct taaaagaaag aatcgcagct tattacaacc actctatttc ggttttagaa
    50401 acaggctcta tcatccaaac acatacagga gaaggagctt ttgcggttat ggttcgttat
    50461 gaataatcgt catttattta gtgggatatt tttctttgtt attagtttat gtctggcctt
    50521 tttattgcta aatattatta tccctaagtc aaattcacgt ttgaaaaaga gtgattttct
    50581 gacaaaagaa caagtagcta tccaatatgt tgctatagga gattcattga cagaaggagt
    50641 aggtgatcta actcatcaag gtggttttgt tcctttgtta acgaatgatc tcagtgaata
    50701 ttttaaggct aatgttaatc atcaaaatta cggcgtatct ggtgatacca gtcaacaaat
    50761 tcttgatagg atgataaaac aaaagcagat acagttatct ttaaaaaaag cagatataat
    50821 gacgttaacc gttggtggta atgatgttat ggcagttatt cggaagaatt tagcggattt
    50881 gcaagtttct agttttagaa agccagctcg tcagtatcaa aaacgattaa gacagattat
    50941 cgagttagcc agaaaagata ataaagatct tcctattttt attttaggca tctataatcc
    51001 gttttatttg aattttccag aactaactga tatgcaaaaa gtgattgatg actggaatac
    51061 caaaactaag gaggttgttg gagaatacga tcgtgtgtac tttgtgccaa taaatgacct
    51121 cttgtataaa gggataaatg gacaagaagg aattgttcat tcttcaggag atcaaactac
    51181 aattgtcaat gatgccttgt ttactgggga ccattttcac ccaaataata ctggctatca
    51241 aatcatgtca aatgcagtaa tggagaaaat aaaaaagcat gaaaaaaaaa tcaaacctta
    51301 attggtggaa gtggagcttc ttatgtctgc ttgcttttaa taccgccttt ttgatggtta
    51361 ttgcaagtcg tctcattcaa gtaagagaac ctgagtcaga actgattgct aaaaaaccag
    51421 ttaaaaatat caaaattggt acatttgtca caaccagaga acagcttaac gaaactgtag
    51481 caagttatct taaagattat cagactaaaa aaatgtctta taaattttat gcgacatctt
    51541 cttctatttt gtttgaagga acttatcaat tattagggta tgaagtccct ttatatatct
    51601 attttcaacc tcatcgtcta gaaaatggag ctgtccaact acaggtgatc tctttttcag
    51661 tgggaactct gccactccca gaaaaagatg ttttgcagta tttgaaatca agctacaaat
    51721 tgccaagctt tgttaaagtg atgccaaatc aatcagctat cgttgttaat ttacaagata
    51781 tccaaaatga tgctaaggtt tatttaaagg ccaagaaaat tgatttattt aatgatgaaa
    51841 tcagttttaa catctataag aaataagctc aaataacgtt aaatacttga cctaatcgct
    51901 ttttttagat atgataatat ctgttaaagc attaagctta ctaattattt ggaggatttg
    51961 ttaaatggct aacaaacaag atttaatcgc aaaggttgca gaagcaactg aattgactaa
    52021 aaaagattca gcagcagcag ttgatgccgt gttttctaca atcgaagctt tccttgctga
    52081 aggtgaaaaa gtacaattga tcggttttgg taacttcgaa gtacgcgaac gtgcagctcg
    52141 taaaggtcgt aacccacaaa ctggtgcaga aattgaaatt gcagcttcaa aagttccagc
    52201 cttcaaagct ggtaaagctc ttaaagacgc tgttaaataa ttcgtctaga aaaatcttgt
    52261 tgctatcgat gtttattgat agcgacaagg ttctttttta tatttggggc atatttgggg
    52321 cataaagtta gtagttttct cttcagttaa gccaagttct aatgcatgca ctctataatg
    52381 gtcagtcatc cttctattag tatatcattt ccgcaggtag ggggagtttt tttcatattt
    52441 gcaaatagtc gaagttagga tttttgaaag caaaaggtaa gtttaaaaaa gtttatttag
    52501 aatgcttcta aattacttga cttatttatt tagaattatt ataattaatg tatcttaaag
    52561 attgaataac ttttaattgc gagggaacta aaaatgacaa tcagacaatg gatggcagac
    52621 catcttcatt taatggagac tttagcgtgt ttggtattaa taataattgg cctagccttt
    52681 ttacactcat ttccacaagt ggcttccgct attttcatta cagccttctt gattggagga
    52741 tatgcgtctg caaaaacggg tatattggat ttggtgaaga acaaacactt gtcagtggat
    52801 attttgatga ttttagcagc tatcggtgct ggaattatcg gctattggct ggagggtgct
    52861 ctgcttattt ttatcttttc gttgtccaat acgcttgaag aaatggccat ggaaaaaagt
    52921 aaggatgcta tttcagcctt gatgtccttg acgccagata cagctcgtca ataccaagaa
    52981 gatggccata ttttagaagt tgagaccaga tccttgagtg ttggtgaccg tttacaagtt
    53041 cgtaaaggcg aagctgttcc aattgacggg cagttgctta gtccttttgg tcaatttgat
    53101 gaatctatgg tcactggtga gcctatcact gttgataagg cagaaggcca ggatctgatt
    53161 gggggaacca ttaaccaagg acaaaccata gatatgttgg ttactattga aaatgacgat
    53221 actctctttg ccaagattat taatctggtg gaatctgccc aagaaaagaa aagcaaaact
    53281 gccaccttta tcgaaagctt agaagatggt tatgtcaaat ttgtgctcgt ccttattcct
    53341 gcctttatcc tctttagcca ctttgtgctt tcttggactt ggttggctgc tttttaccga
    53401 gggatgattc tcttaacagt agcttcacca tgtgccctaa ttgccagttc cacacctgct
    53461 agcttggctg ctatttctcg tgcagccaga aagggattga ttatcaaagg aggggatatt
    53521 gtcgataaca tgggagatat taaggctgtt gtcatggata aaacgggaac gctcacccaa
    53581 ggaaaacctt ctgttgtgaa tgctcattat ttggaagatg agttgctcgt gaatagactg
    53641 gtaaaaggag cagagactgc tagtacccat cctatttcta aagcccttct tgaatacact
    53701 gaaaaattgg agccactgac ctttgaccac ttagaagaaa tttctgggaa aggttttcag
    53761 ggcttctatc aagggcaaga atggcgaatt ggcaagaaaa ccttcatttt ggaaaaggtt
    53821 caagacctat cagcttttga agaaactatt caagtggaag aaaatcaagg gaaaacccta
    53881 atctttgttt cacgtgacca tcaattgata gcttactatg ccctcttgga tgatatcaaa
    53941 atagaatcaa aacgtgctat taagtctctt catgccatgg gaatcaaaac agtcatgtta
    54001 acaggtgacc aagaacgaac cgccaattat gtggcacaaa aacttggtat tgatgaagtg
    54061 gtagccaact gtatgcctca agataaggtg gctaagttag cagaattaaa gaccaaatat
    54121 ggttttgtgg ccatggtagg agatggtatt aatgatgctc ctgcccttgc tcaagcagat
    54181 gtttcttatg ctattggatc aggaacagat attgcaatgg aaagtgcaga cagtgtgatt
    54241 atggatgact tgactcgtat tccattttcg attcaacttt cccgcaaaat gaagaccatt
    54301 atcaaacaaa atattgtttt tgccttatct gtgattacct tattgatttt agccaatgtt
    54361 tttcaggtag ttaacttgcc gcttggtgtt gttggacacg aaggctcaac gattttagtg
    54421 attttaaatg gcttgcgttt actttctttt aaataattta gaaacttgct tgaaaacttg
    54481 aaagtgttta taataatagt aagacttatg aaagagaaaa ccgccgtatg tttactatta
    54541 tgatcattgg tttagctgta ttttcaataa ttaaaatcat tctatttttc tatttttatc
    54601 agcaatttga gcgcaagcaa aaaagaaaag ttgaatcaaa caaccagcaa agacagtccc
    54661 aagaaagtga cgctgtccct tcaaaaaaat tcccatttta aaaagcttgg aaagtgtaca
    54721 aagtgcacat ttccaagctt ttttgactat taaggttaat ctttgtaacg taagttccca
    54781 cgaaaatcgt ctaagctttg gtagcctttt tctaccataa tcgtttttag ttccttagtg
    54841 acccgttcaa agatagctgg cccttcttga tgaagggctg tcccaatttg gaccatgctg
    54901 gcgccacaaa gaatatgttc aaaggcatcg cgtcctgttt taactccccc tgtaccaata
    54961 atgtggatag aaggttttaa gcgtttgtaa aacgcatgga cattggctag agcagtaggt
    55021 ttgatgtaat ctccacctat accaccaaag ccatttttag gcttgatgag gacttgttca
    55081 tcctcaatga ccagcccatt tccgatagaa ttgacacagt taacaaagga aagggggtat
    55141 ttattgaaaa tcgcagccgc ttgatcaaaa tgcacaatgt caaaataagg aggtaatttg
    55201 ataccaagag gtttagtgta ataggtaaag atgttctcta gaagttgatc agtggtttca
    55261 aaatcataag caatctgtgg ttttcctgga acgttagggc aagaaaggtt tagttccact
    55321 agaccctcat aatctgaagc catgatggct tttaaaatgg tttcggtttc agtaggagat
    55381 agcccgacaa cggagagaaa gtgaggttta tgttgcccgg ttttggcaag atcactgaca
    55441 aagtctaagt agtagcgaaa accattatta gggagtccca ttgaattgat agaaccaaga
    55501 cgagtatctg cgtagcgagg ttctggatta cctggacgaa cttcaagcgt ccctgttttg
    55561 gtgacaaagg aagcggcttg agacttttca acctccataa gctcttcttt tgtcatgcag
    55621 taaacaccag cagcattcat caaacagtta tcaaaagaga aatgaccaat ttgcgtagca
    55681 gtagaaacca ttatttcctc catattttag acaaattttt tctattctac cactaggcat
    55741 gaataatagc aagctatttt cacaaaaatg taaatatatt actgttattg acgaacattt
    55801 gaaattttca tgatagtcac attatgattt tcaataaagt cagaatagac tagctttttt
    55861 gctatatttt ttgtaaaata ggaattgtaa aaaggtagca acctttaaat taaaaggaga
    55921 tattaactca tggtaaaatt ggttttcgct cgccacggtg agtcagaatg gaacaaagct
    55981 aacctcttca ctggttgggc agatgttgat ctttcagaaa aagggacaca acaagcgatt
    56041 gatgcaggta aattgatcaa agaagcaggt attgaatttg accttgcttt cacttcagta
    56101 ttgacacgtg ctatcaaaac aactaacctt gcccttgaaa atgcaggtca attgtgggtt
    56161 ccaactgaaa aatcatggcg cttgaacgag cgtcactatg gtgctttgac tggcaaaaac
    56221 aaagctgaag ctgcagaaca atttggtgat gaacaagttc atatctggcg tcgttcatac
    56281 gacgtattgc cgccagctat ggctaaagat gatgagtatt cagcacacaa agaccgtcgt
    56341 tatgctgatc ttgacccagc tcttattcca gatgctgaaa acttaaaagt aactcttgaa
    56401 cgcgcaatgc cttactggga agaaaaaatt gctccagctc ttcttgatgg taaaaacgtc
    56461 tttgttggcg cacatggtaa ctcaatccgc gctcttgtga aacacattaa aggtctttca
    56521 gatgacgaaa tcatggatgt ggaaattcca aacttcccac cacttgtttt tgaattagat
    56581 gaaaaactta acattgttaa agaatactac cttggtggtg aataacttaa aaaccagact
    56641 ttggtctggt tttttggttc tatatcctaa gaagaaagaa aaccctgctt aaaaatatga
    56701 tatgatagaa ataaaaaagg atagatagat tatgaaagca gaagatcgtc gtcaaaaaat
    56761 tattgagtgc ttaaatagtg aacaaaaagc tgtatctgct actcgtttag gtaagttatt
    56821 aggagtgagt cgccaagtaa tcgttggaga tatagctctt cttcgagctc agcagattga
    56881 tattatttcg actccaaaag gttatattat gtcaacagcg ctttatagtc accaattttg
    56941 tgcacgtatt gtgtgccaac ataatgtaga agaaactaaa aaagaattag aaattatttt
    57001 agctcatcaa gggattatta cgacggtaga ggtagaacat cctatttacg gtatgataac
    57061 agccccttta aatattaaga cacactctga tgtaactaat ttcatgtcca aactcagtca
    57121 atctaaagca gagttattgt cttcgttgac agaaggacta catagtcact taatttcgtg
    57181 tcctagtcaa gaagcctttt tagctatcaa gcatgattta gaattagcag gtatcttata
    57241 caaaggaata taactatagt atattatgcc attgatggtt gttatgaata ccatgctaaa
    57301 atattctatt gattatttat cagcaacaag gtatcgttta aaaataccaa aagatacagg
    57361 tgtcttgata gttattggtt tttaaggtat actatttttg aaaaggagat aaaatgacta
    57421 aaaaaccaac acaactaata gcttatacct ctattttggt cgcatttgcg attttgattc
    57481 cgataatcat gcctcttaag cttattattg gaccagcttc ctttactctg gcgagccatg
    57541 ttcctttgtt tttggctatt tttatgtcga ttccagtagc aattttggtc gctcttggta
    57601 ccacattagg ttttttatta gctggacttc ctttaattat tgttttaaga gcactctctc
    57661 atttattgtt tgctattttg gcagcgtggt ggttaagtag aaaaccacaa ctgatgacat
    57721 cggcagtaaa atgttttagt tttgcgtttt tcattaatgt cattcatggc ttggctgaat
    57781 ttttagttgt ctatatttta acagcaacca cagcaacttc tatgtcttat ttttggtcta
    57841 tgctaggctt gattggctta ggttcgctta ttcatggcat attggatttc tatcttgctt
    57901 tagttctatg gcgtttttta gcaaaaaact taaaacttcc caccataaat gcctaagact
    57961 aaaatagaga tagttatatg ataatgacga gccctttctt gttgaaaggg cttgcatttt
    58021 ttaggaaaat tgatatacta aagtgtgtgt ttgtgaatac agtaacaaaa ggagatatca
    58081 tgaaaacacc agaacagatt ttagaagcaa ctattcatat tggggaacat aaggttacca
    58141 aaactttttt ggctaaatct atcttaggct ttattggtgg agcgatgatt agccttggtt
    58201 atttactcta cgtgagaatt gccgccagtg gtttagaaac ttttggtgct ttttccagta
    58261 tcgtaggagc ttgtgctttt ccgattggtc taattattat tttaatggcg ggtggagaat
    58321 taattacagg gaacatgatg gctgtttctg cagctttgtt ggccaaaaaa attaagttta
    58381 gcgagcttgc caagaattgg cttatcatta ccttgtttaa tgtgattgga gctgtttttg
    58441 tagcttttgt gtttggtcat tttctaggct taacatcagc tggtattttt aaagaagaag
    58501 tgattgaggt ggcccatgct aaaatagcag cgagcccttt gcaggcacta gtatcaggaa
    58561 ttggttgtaa ctggtttgtt ggtttggcct tgtggctatg ctatggggca aatgatgctg
    58621 ctggtaaatt ccttggtact tggtttcctg tcatgacctt tgtagccctt ggttttcagc
    58681 atagtgtagc aaatgctttt gtcattcctg ctgctatttt tgaaggagga gcaacatggt
    58741 tggattttgc cactaatttt atttttgtct atagtggtaa tattatcggt ggagccattt
    58801 ttgttagttt tttatatttt aaagtttact atcatccgca aaagagtaaa actcaatgag
    58861 aaaatatttt tttagactga gggcgtccta ttctcagtct tctttttata atttatggta
    58921 aaataagaag cgaagtttaa aaaatttagc taaataatgg ttagtttatt ccctaaaaaa
    58981 ggagtattcc caaaaaatcc gtaaaaagaa tttgctatgg gagtagttcc caaaataatg
    59041 cagatcatta gttgtctttc aaaaatagcc ttgatagggt aaaaagtgga cagtgatgca
    59101 gcaacagatt catcaggcgt acaaactgtc ttatggtttg tgcttagaga tttcttaata
    59161 atgcttttgg taatacactt ttagtgaaga aagtgtagca tgaaagctta aagataacca
    59221 tgagttcgct ttatgcactc ttgttgagaa aaatagcata gtcaattgag taaacatcat
    59281 ttctgcatgg aatgaaacaa ttcctataaa cttatatcat aaggatggtt tgcggtttct
    59341 aaaaaagtaa aatgaatcaa aaaacaaaag ccaaatatct caaggccaag gtaacaagtc
    59401 gagtaaaaaa gtttttactt ggctaatcca aacggttctg tcacgattca cgctaaaatt
    59461 ggaactgctg agacttgtat cacagacaat tcagatcaga ccttagtaac ttacaactta
    59521 aatgtagtag cttataatac aaataacgaa gataatattg cggttgctgt tatgtatcca
    59581 catattacga atatatcggc aaagatgtga gggcgcttta tgtggcgata tatggaaaac
    59641 aataaaggag aacttagtgc tttatccaac acccattgca aagttaattg acagttactc
    59701 taaacttcca ggaattggta tcaagacggc gacgagatta gccttttata ctattggaat
    59761 gtcaaatgaa gatgtcaatg attttgctaa aaacttatta gcagctaaaa gagaactgac
    59821 ctattgttcg atttgtggaa accttaccga tgacgatcct tgtcacattt gcacagacac
    59881 gagccgtgat cagacgacca ttctggtagt agaagatgct aaagatgttt ctgccatgga
    59941 aaaaatccaa gagtatcatg gctattatca tgtgcttcac ggcttgattt cgcctatgaa
    60001 tggtgtgggg ccagatgaca tcaaccttaa aagtttaatt acccgtctaa tggatggtaa
    60061 ggtgagcgaa gttatcgtag ctaccaatgc cacagcagat ggggaagcaa cgtccatgta
    60121 tatttcacgt gtcttgaaac cagccgggat taaggtaact cgtttggcaa gaggtctcgc
    60181 agtaggttca gatattgagt atgctgatga agtaacatta ttgagagcta ttgagaatcg
    60241 tactgaactt taatgatcat gttggtgcct ttactcgcaa agagaggttt tgaaaaggct
    60301 acatcttttt gttataataa aaaaacattc aattcagata agaaaggtta gctatgtcca
    60361 aacaaacgtt agttctatta tacggaggcc gctcagcaga acgtgaagtc tcagtgcttt
    60421 cagcagagag tgtgatgcga gcagtcaact atgataagtt cttagtaaaa acttatttta
    60481 ttactcagat gggccaattt atcaaaactc agcaattttc agaaaagcca tcggaatcag
    60541 aacgcttgat gacaaatgag acgatagagc taactcaaaa aataaaacca agtgatatct
    60601 atgaagaagg tgcagttgta tttcctgtat tacatggacc tatgggagaa gatggctcta
    60661 tccaaggttt cttagaagtg cttagaatgc catatatagg gactaatgtc atgtcttcta
    60721 gcatagctat ggataaaatt actactaaac gtgtcttaga atcaattgga attccacaag
    60781 ttgcctatac tgtttatatt gacggacaag atttggaggc ttgtttagtt gaaactttag
    60841 caagacttac ttttcctatc ttcgtaaaac cagctaatat gggttcttct gtggggattt
    60901 ccaaagccca aacaaaagta gaattaagaa aagcgattca gttagccctg acctatgaca
    60961 gtcgtgtctt gattgaacaa ggcgtggttg ccagagaaat tgaagttggt ctccttggaa
    61021 atgataaggt taaatcaact ctaccaggag aagtaatcaa ggacgttgat ttttacgact
    61081 accaagcaaa atatgttgac aataagatta cgatggccat tccagcagat gtagaccaga
    61141 gtattgtgac agaaatgcgt tcttatgctg aagtagcctt taaagcctta ggtggttgtg
    61201 ggttgtcccg ttgtgacttt ttcttgaccc aagacggaca ggtttacctt aacgaattaa
    61261 acaccatgcc aggctttacc caatggtcta tgtatccact cttatgggaa aatatgggat
    61321 tggcttatcc agatttgatt gaagagctgg tgacactggc acaagaaatg tttgaccagc
    61381 gtgagagcca cctgatttaa acctatgaga tgaataaaca ttgacttgga tggaacggca
    61441 aacatcgtca agatgtgacg ttcgtccttt ttttgttgct cttttaggaa gaatagtaga
    61501 gccattaagg gagtttgaca ttttagaaat ttttctaatg ttttgtgcta taatagatag
    61561 gacatcgtaa atactagtgt tcttagaaag aaaacaaaat gaaattaacc ttacacgaag
    61621 tggctaaaat agttgacgcc caaaataatg tgtctgactt agacgacgtt cctctgcatc
    61681 acattgaatt tgatagccga aagattacaa aaggagacct ctttttaccc ttaaaaggtc
    61741 aaagggatgg tcatgagttt attgatttag cctttcaaaa tggagctgtc gctacttttt
    61801 cagaaaaaga attgccgggg aaacctcacc ttcttgttga agactgccta aaagcttttc
    61861 aaaagctagc ccattattat attgataaga tgcgcgtgga tgttattgct gtgacaggct
    61921 caaatggtaa aacttccacc aaagatatga taggagctgt cctatcaacc acttacaaaa
    61981 cttacaaaac gcaaggaaat tacaataatg agattggttt gccttacacc gttctccaca
    62041 tgcctgacga tacggaaaaa attgttcttg aaatgggaca agatcacatg ggagatattc
    62101 gtcttctgtc tgaaattgca aggcctcgta ttgcagtttt aaccttagtc ggcgaagctc
    62161 atttagaata ttttggaagc cgagacaaaa tagcccaagg caaaatgcag attgttgacg
    62221 gcatgaattc agatgggatt ttaattgctc cgggagatcc gattattgac ccatatcttc
    62281 cagaaaatca gatggttatc cgttttggaa atcagcagga aattgacgtt actggtattc
    62341 aagaagacaa agatagctta acgtttacaa caaatgtgtt agcaacacct gtgtccctac
    62401 ctctaccagg aaaatacaac gcgaccaatg ctatggtagc agcctatgta ggaaaactat
    62461 tggcagtaac agacgaggat attatagctg ctttacagac tgtgacgttg acaggtaatc
    62521 gtacggagtg gaaaaaagca gccaatggtg ctgatatttt atcagatgtc tacaatgcca
    62581 acccaacagc catgcgtctg attttagaaa catttgctaa catagcaaag aaccccggag
    62641 gaaaaaagat tgcagtgcta gcagatatga aagaacttgg gaaggattca gtgatacttc
    62701 attctcagtt aattgacagc ttaacatcag gtaatattga tcagcttgtt ttttatgggg
    62761 atcatattaa agaattagca cggttggcaa gtcaggttta tcctgctgag caagtacatt
    62821 attttttaaa aactgaacaa gaagaccagt ttgaagccat ggcacaatat gttcaaaaca
    62881 ttcttaatcc ctttgatcaa attcttttaa aaggcagcca ctcgatgtcg ctagaaaagt
    62941 tagtggatcg attagtgact gacaagaatt gtgatagcta tcagccttga tttttataaa
    63001 actttcagat aaagtcttgt tctctcatga agattaaggt ataatgaaaa aatatagaaa
    63061 gggacgatta tggatttttt tgcaattgac cccatcggac tgcctcatac ctcacttatc
    63121 ttttacttgt cgtcactctt gatagcgctt ttgcttgttt ttttgacttt tcaagcatat
    63181 aggttaaaat cttatcgata ttttttcttg tttttacagt tatctcaggt gattggcttg
    63241 tacacatggt acgtgttaag ggggtttcct ttagatgaag ctttgccgtt gtatcattgt
    63301 cgcatcgcta tgttagctat cttttttcta cctgatcgta acaagtttaa gcaattattt
    63361 atggttttgg ggataggtgg aacattcctt gcacttttat cgccagatct ttatccgttt
    63421 agattatggc atgtagcaaa tgtctcgttt tatcttggtc attatgcttt gttagtgaat
    63481 ggtttgattt acctcttgcg tttttacgat gccagtcagt taagactgct atcagtagta
    63541 cgctatttag caactgttaa ctttcttctt ctgttggtca gtttagccac gaaaggaaat
    63601 tatggttttg tgatggatat tccagtgatt catacgcgtc atttgctact taattttgtg
    63661 atcgttacaa gtggcttgac ttttatggtt aaaataaccg aatattttta ccttaaattt
    63721 ggtgaagctc aacagctggc atttgctttc tctaaagaaa agtaggtcgt tgaaaaagca
    63781 cttaaaatca gctataataa gaagagtctg ggatgatttc caggctcgat ttttttaaaa
    63841 aagatactca ttgatagccc aagataagtt ataataccag ttttgaagaa ctttagaggc
    63901 taatcgtata tggagaaacc caatgtagcc caactctttt gataaacaag taatacctaa
    63961 cgaaaataac tcagagaaaa gctctgccat tttactatac aaggaaaaaa gaatgtcact
    64021 tacagaagaa attaaaaaac gccgtacttt tgccatcatc tctcacccgg atgctggtaa
    64081 gacgactatt actgagcaac tattatattt tggtggtgaa attagagaag cagggacagt
    64141 aaaagggaaa aaatcaggta cgtttgcaaa gtccgactgg atggatattg aaaagcaacg
    64201 tggtatctct gttacttcat ctgttatgca atttgattac gcgggtaaac gtgttaatat
    64261 tttggataca cctggacatg aggatttctc ggaagataca taccgtacct taatggctgt
    64321 ggatgcggct gtcatggttg tagattcggc taaaggtatt gaggcgcaaa cgaaaaaact
    64381 ttttgaagtt gtcaaacatc gtaatattcc tgtcttcacc tttattaaca agttagaccg
    64441 tgatgggcgt gagcctttag aactcttgga agaattagaa gaagttttag ggattgcttc
    64501 ttatccgatg aactggccga ttggtatggg acgcgccttt gagggcttat atgatttaca
    64561 taataaacgt ttagaactct ataagggaga cgagcgtttt gcaagtattg aagacggtga
    64621 tcagctgttt gccaataatc ccttttatga acaagtcaaa gaagatattg aacttcttca
    64681 agaagcaggt aatgtctttt cagaacaagc tattcttgat ggtgacctaa ctccagtctt
    64741 ttttggctct gcccttacga actttggagt tcagactttt cttgatactt tcttggagtt
    64801 tgccccagaa cctcatggtc acaagactac agaaggtaat gttgttgacc ccttagctaa
    64861 agatttttca ggttttgtct ttaaaattca agctaacatg gaccctaaac accgtgaccg
    64921 tattgctttt gtgcgtattg tttctggtga atttgagcgc ggtatgggag ttaatttgac
    64981 acgcactggc aaaggcgcta agctctccaa tgttactcaa tttatggcag aatcacggga
    65041 aaatgtgacg aatgctgttg ctggggatat tatcggggtt tatgatacag gaacttatca
    65101 agttggggat accttaacag ttgggaaaaa taaatttgaa tttgaaccct taccaacttt
    65161 taccccagaa atcttcatga aggtttctcc gaaaaatgtt atgaagcaga aatccttcca
    65221 caaaggtatt gaacaattag tacaagaagg agcaattcag ctttataaga attatcagac
    65281 aggcgaatac atgctgggtg ctgtgggtca actgcagttt gaagtcttta aacatcgtat
    65341 ggaaggtgaa tacaacgctg aagttgtgat gacaccaatg ggtaaaaaga ctgttcgctg
    65401 gatttcagaa gatgatttgg atcaacgcat gtcctcaagc cgaaacattt tagctaagga
    65461 tcgttttgac caaccagttt tcctatttga aaatgatttt gcccttcgct ggtttgcaga
    65521 caaataccca gatgtcactt tagaagagaa aatgtaatcg aaacatgatt ttcaacagtc
    65581 taacgttttg gagatcaatt tactgagcaa ctttttaaaa gaaatgagtg gctagttatc
    65641 tcttttgcag atttaagatt ctatcaaaaa acgatatctt cacaactgta agcatattta
    65701 tgatttagcg gataatcgtg aaagcgtgtt attgcttata ttagccttat ttcctatctt
    65761 tttgattcta attgtgttac actagttagg ttgttaatca aacgacgaaa attaaaaggt
    65821 ttataaggca gcttcgcact gcctttttag aaaagagaaa caattgaaat ttacagaatt
    65881 taacttatca caagacatcc aatcagctgt tgttaccgct ggttttgaga aagcatctcc
    65941 tatccaggaa atgacgattc ctctcgcact agaagggaag gatgttattg gtcaagcaca
    66001 aacaggaact ggtaaaacag cagcgtttgg tctaccaacc ttgaataaga ttcgtactaa
    66061 cgaaaatatc atccaagcat tggtcattgc accaacgcgt gaattggctg tacaaagtca
    66121 ggaagaattg ttccgttttg gacgtgaaaa aggggttaag gttcgctctg tttacggtgg
    66181 atcaagtatt gaaaaacaaa tcaaagccct taaatcaggt gcccacattg tcgtgggaac
    66241 accgggtcgc ttgttagatt tgattaagcg caaggctctt attttagatc atgtggaaac
    66301 cttaatcctt gatgaagcag atgaaatgct taacatggga ttcttggaag acattgaagc
    66361 tattattagt cgtgtcccag cagaccgtca aaccttgctc ttctccgcaa cgatgccggc
    66421 acctattaaa caaatcgggg ttaaattcat gaaagaccct gaacatgttc aaattaagaa
    66481 caaagaatta acaaacgtca atgttgatca atactatgtt cgtgtgaaag aacaagaaaa
    66541 atttgatact atgacccgtc tgatggatgt taaccagcca gaattatcca tcgtttttgg
    66601 gcggactaag cgccgagttg atgaaattac acgtggcttg aaacttcgtg gtttccgtgc
    66661 cgaaggaatc catggtgatc ttgaccaaaa taaacgctta cgtgttatcc gtgatttcaa
    66721 aaacgaccaa attgatattc ttgttgccac tgatgtagca gcgcgtggcc ttgatatttc
    66781 aggagttacc cacgtttata actacgatat tacccaagat ccagaaagtt atgttcaccg
    66841 tattggtcgt acaggtcgtg ccggtaaatc tggtgagtct atcacttttg tctcaccaaa
    66901 tgagatgggc tacctctcaa tgattgagaa cttaacgaaa aaacagatga aacctcttcg
    66961 tccagcaact gctgaagagg cctttcaagc caagaaaaaa gtagccctca agaaaattga
    67021 acgcgacttt gcggatgaga ctattcgttc aaactttgat aaattcaaag gcgatgctgt
    67081 ccaattggct gccgaattca ctcctgaaga attagcttta tatattttga gcttaacggt
    67141 tcaagatcca gatagcttac cggaagttga aattgcacgt gaaaaaccat taccattcaa
    67201 atatgtgggt ggtggtcacg gcaataaaaa tggcaaaggt ggtcgtggac gtgacaaccg
    67261 taaccgtggt gatcgccgtg gtggctatcg tggtgaccgt aacagagatg agcgtgatgg
    67321 tgaccgtcgt cgtcaaaaac gtgataagcg tgatggtcac gatggcagtg gcaaccgcga
    67381 tttcaaacgt aaatcaaaac gtaatagtaa agactttttc aataaagaga aaaaatcaag
    67441 tgctaaaaat actggttttg tgattcgtca caaaggtgaa taagcttaaa gagagttgat
    67501 gagagtcagc tctttttaat tttctgaaaa agttgctttt ataatgggat ctgttataat
    67561 ggtttcagtt atttcattat aatttttaaa aaattaggag gagtataaga tgtctgatag
    67621 ccatcttact gcctttgaca aagcttcaaa agcaggtttt attattgctt taggtattgt
    67681 ttatggagat attggaacaa gcccccttta tacgatgcag tctctagttg aaaatcaagg
    67741 aggggttaat caggtttctg aatcctttat tttaggatca atctctttaa ttatttggac
    67801 actgaccttg atcacaacaa tcaagtacgt tttgattgct ctaaaagctg ataaccacca
    67861 tgaaggtggg attttttctt tattcacctt ggtacgtaaa atatcaccgt ggctcattat
    67921 tccggcgatg attggaggtg caacactttt gtctgatggg gctcttacac ctgcggtaac
    67981 agttacttca gctattgagg gcttgaaagc cgttcctggt ctgagtcaca tctaccaaaa
    68041 tcaaaccaat gtcattatca caactctagt gattttaata gtcttgtttg gtattcagcg
    68101 gtttggaaca ggttttattg gtaaaatttt tggaccagtt atgtttatct ggtttagttt
    68161 tctaggtgtt agtggttttt ttaatacgtt ggggcatctg gaaattttca aggctatcaa
    68221 tccttactat gctctgcatt tgctgtttag tcctgaaaac catcgtggga tttttatctt
    68281 ggggtccatt tttcttgcga caacaggagc tgaagccctt tattcggatt taggacatgt
    68341 tggtcgagga aatatttatg tgagctggcc ctttgttaag atgtgtattg tgttgtctta
    68401 ctgtggccag gctgcgtgga ttttagcgaa taaacactca ggtattgaac taaacccttt
    68461 ctttgctagt gtgccatcac aattaagagt ttaccttgtt agtttggcaa ccttggcagc
    68521 tattattgcc tcacaggctc ttatttcagg ctcctttacc ttgatttcag aggccatgcg
    68581 tttgaaaatt ttccctttgt ttcgtgtgac ttacccaggg gcaaatttgg ggcaattgta
    68641 tattcctgtc attaactgga ttttatttgc agtgacctct tgtactgttt tggcttttag
    68701 aacatcagcc cacatggagg cagcttatgg tcttgctatt accatcacca tgttgatgac
    68761 aacaatcctc ttaaagtatt acttaatcaa gaaaggaacg agacccatct tggctcattt
    68821 agcgatggct ttctttgccc ttgtagaatt cattttcttc ttggcttcag ctatcaaatt
    68881 tatgcacggt ggctatgcgg ttgttatctt ggcacttgca attgtctttg tcatgtttat
    68941 ctggcatgcg ggcacacgga ttgttttcaa gtatgtgaaa tctcttaatt tgaatgatta
    69001 caaggagcag attaaacaac ttcgtgacga cgtttgtttt gacctctacc aaacaaatgt
    69061 tgtctactta tccaatcgta tgcaggatca tatgattgac cggtctattt tgtactccat
    69121 tttagacaag cggccaaaac gcgctcaagt ttattggttt gtaaatgtcc aggtaactga
    69181 tgaaccttat acagctaagt acaaggtaga tatgatggga acggattata tggttcgagt
    69241 caacctttat cttggtttta gaatgccaca gactgtgcca cgctatttgc gcacgattgt
    69301 tcaggatttg atggaaagtg gccgtttacc caaacaagaa caggaatata ccatcacacc
    69361 tggtcgtgat gttggtgatt ttcgctttgt tttaatagaa gaacgggtca ccaatgcacg
    69421 tcaattgagt aactttgaac gctttattat gcagactaag gcaagcatta aacatgttac
    69481 cgctagtcca atgcgttggt ttgggcttca atattccgaa gtaacccttg aagtggttcc
    69541 cttgattcta tcagatgtct tgaagttacc aatcaaagaa ttggtgcctg tggaagacag
    69601 tgaagcataa aaaaatcatt gagatgataa gtctcaatga tttttctttt ctttttgatg
    69661 agtagcaata taggccaatt tctggcttct tgtttttcgt tttttaaaga gtgcttcagc
    69721 gctcatcgca gcctctttgc tatcaaacac ttcgtaataa agtaaagaaa caggtaaccg
    69781 gtagcgagta tatttagccc cctttcctgc gttatgtgta gctaaacgct ttttcaaatc
    69841 agttgtataa ccagtgtaga gggttttatc tgcacattct aacacataca tataagcttt
    69901 tttagtggtc atgattaggt tttcctttgg aagcagcacc aaaataaatc tcaaaaatat
    69961 gatcagtgta ttctccgttt tccttgtgga caactaaagg aggtaaaata gtcatccctt
    70021 caatagaacc gtctttgatt gcttcaataa gtaacatatt agctgattta cctaatttag
    70081 gatagacaaa ttgtaccctt ttaggggcaa gaccattagc tctaaggcta tctataattt
    70141 ccaagaatcg atctggtcga tgaaccattg ccagacgacc atttgattta agggcatgac
    70201 gggctacctg acaaatctct tctaaattag tggtcacttc atgacgagcc agtaaataat
    70261 gttcagagac gttcttctta gaactttcgt gacttttaaa gtaaggggga ttgcagagca
    70321 tcaaatcaac tcctgaacgt ggcacatggt ttaggaggtt ttttaaatca tcacaaatca
    70381 tggttacttg gtcttccagt tgattgagct ggatagagcg ttgtcccata tcagcaagcc
    70441 gttcttgtaa ttctacttca acgatggctg ccttggttct agtgctagca aataagccaa
    70501 ccgccccatt tcctgagcac aaatccacaa ttaacccttt ggaaggcatc ttggggaagc
    70561 gggataagag gacgctatca atagagtaac taaagacatc cttgttttga ataatgccaa
    70621 catcacttga aaagagttga tcaatacgtt cgccttcttt taagattgct tttatcatac
    70681 ctgttatttt aacataaaat aggcttgagg tgaatgtttg aagagtgatt ttgactttcg
    70741 aaaagtcagc tttcatgata caataagtaa gattattcta ttaaaggaga agcctgtgtt
    70801 ttacgcttac ttacgcggtt tagtagtctt tttattatgg gttgtcaatg gcaatgccca
    70861 ctaccatcat gaagaaaaga tgttagatgc ttctgagaat tatattctgg tagcacccca
    70921 tcgaaccttt tgggatccag tttacatggc atttgcagca cgtcctaagc agtttatttt
    70981 tatggctaaa aaagagttgt ttgcaaatcg tctctttgct tggtggatta aaatgtgtgg
    71041 tgcttttcct attgatcgtg acaaaccgag tccagatgct attcgttacc ctgttaatat
    71101 gctcaaaaag agcaatcgtt ctcttcttat gtttccaagt ggaagtaggc attcgcaaga
    71161 ggtcaaagga ggggtcgctg tgattgctaa attggctaag gtcaagatta tgccagcagc
    71221 ctatcagggg cctatgtcgg tcaagggatt actagcggga gaacgggttg acatgacttt
    71281 tgggaatccc attgacgtct cagatattaa acgcatgaat gatgaaggga ttgctgaggt
    71341 ggcaaaccgt attcaggcag agtttgatcg gatggatgac gagttggcac ctttccaacc
    71401 aggaaaagca aggaaccctt taacttatct ctaccgttta ccattaggat tggttttggt
    71461 ggtggtatta ttattaacca tattattttc ctatattgct agttttgttt ggaatccaga
    71521 taaacatcgc cattaagaac atgagggcta acatgtgcct gaaataagaa gtgattgaga
    71581 ttagtcttct attgaaagaa gccgctaatg cttaatcact tttttcattt ctaaaaaaga
    71641 tattcgatga tacccttaac tcttttaagc acaaaaaatg acaatactac tatacttttg
    71701 gatatcattt acgaataaaa agatgaggta aaaaaatgtt agacgaatta ttaattaaag
    71761 gaaaatcgtt tttgagagag cggtattttc tgccatacct aattagtctt ttattaggac
    71821 tttttctaat actaagcttc agttttttgt tctggggaaa taggcagagc aaggcagccg
    71881 ttcctgcttt gagagaaata tcgcctgtga aacagcaggt tagtgaagaa aaaaaggaaa
    71941 ttcaagaaga tagcagtatt ttagtggatt taaaaggcgc cgtacaaaaa gagggagttt
    72001 ataagctaac tgctagcagt cgtgtgcgag atgtcattga actagctgga ggcctaactt
    72061 cagaagctga taaacatgct attaattttg cagaaaaact aactgatgag caagttgttt
    72121 atgtacctaa acaaggagaa gagatttctg tgctaccaag atcattagtt tctggtaaaa
    72181 aagaaactgc cagcaaggat cagtcaaagg ttcatattaa taaggccagc ttagaagaac
    72241 tacaacatat cccaggtatt ggtgctaaaa gagctcagga tattattgat atgagagata
    72301 agttaggtgg ttttaaagcg ttagaagatc tccgtcaggt ttcggggatt ggcgaaaaaa
    72361 cgctcgagaa attaaaagat gacattttct tggactaagc tagtgcccct gtcaaagatt
    72421 caatttgctt ttttaatctt ggttctcttt tatcaaatcc atagccctag ctggttaacg
    72481 tttctcctta gccttagttt gatttgctta cttgtcaagc gtttgtctaa aaaagaattt
    72541 ctcggtgtct ttgccatcct tagtttctgt gctttgtttt tgctgtatca gaaacagcag
    72601 ttgattcaaa aactggaaat tcagccagta caaataacct ctgtagcatt agttcctgat
    72661 agtattcgga ttaatggtga tcaattactg ttcttggacg tcacgggaaa cattcttatc
    72721 agttatttta ccgtttgaaa agccaagcag aagcccaatt gttcaagaaa gagcaccgtt
    72781 ggttagtgat gcacgcaaaa gtgacattgg aaaaagctga ggaagttcga aattttaaag
    72841 gctttaatta tcagactttt ttggcttatc aaggaattta caggataggg aaggtagagc
    72901 aaatagaaca gcttgaagtg atttcacccg agtcaatatg tgactatttg tcctctttaa
    72961 gacgacgagc cattgttcat tgccagcagc attttccaag acctatgtct cactacctga
    73021 cgggcctcct ttttggttat ttagataagt cgtttggtga gatgacagat tattatagtc
    73081 agttgggtat cattcatctc tttgctttat cagggatgca ggttggcttc tttcttactt
    73141 gttttagaag ggtgttgcta ttactcgcag ttcctttgga atggataaaa tggatagagc
    73201 ttccttttgc ttgcttttat gccgcgttga caggttatag tatttctgtt attcgaagcc
    73261 tcgtgcagtc gcagttgcgt catttaggaa taaaaggatt agacaatctg gcttgtactt
    73321 ttttacttgt ctttttatgg gatgctcatt tcttaatgac tgttggtggc gtattaacgt
    73381 ttagctatgc ttttttgtta acagtggtta ctgttgaaga attatcagga gctaaaagac
    73441 agttggtaca agtcttgacc atttccctag gaatattacc atttttgctc ttttattttt
    73501 ctagttttaa ccccatgtca atggtcttaa cagggctctt gtcttatctc tttgaccttt
    73561 ttattttgcc ccttctttgt ctggtttttt gtctatcccc tttggtgact gtctcaatat
    73621 gtaaccatct ttttatttta ttagaaaaag tcattcaatt tctcggaaat actttcaata
    73681 gttcccttgt ttttggaagc ccgaccagtt ggcacttgct tatcttggtg attagcttcg
    73741 ctatttttta tgattatcgg caggtgcgac aacgagttat cacttgcggt ttggttatag
    73801 cgttgacgct tttgtctgtc aaatatcctt tgaccaatga agtgaccttt atcgacattg
    73861 gccagggaga tagtattttg gtgagggagt ggacagggaa aaatctctta atagatgtag
    73921 gtgggcgccc tttcttttct tcaaaagagc actggcgacg aggacatcac gtagctaatg
    73981 ctcagaaaac cttaattcct tacttaaaaa gtagaggtat tcacacaatt gaccaactat
    74041 tagtaactca cgcagatact gatcatatgg gggatatcga agtggttgcc aaagctattc
    74101 gaataaagga gattttgact agtcaaggca gtttaagcca tccaagcttt gttaggcggc
    74161 taaggcgttt aaaatgtcac gttagggttt tagcagctgg agaccagttg cctattatgg
    74221 gaagtgtctt acaggtactc tacccttggc agttagggga cgggaaaaat aacgattctt
    74281 tggtacttta tggcaggctc ttaaatcgaa cttttctttt cacaggtgac ttggaaaaag
    74341 agggggaaaa cgagatcata aaacgttatc ctcaactaag agtagattat ttaaaagcag
    74401 gtcaccatgg atcaaatacc tcttctagtg cagctttttt agaccatatt cagcctaaag
    74461 tggcctttat ttctgctgga aaaaataacc gttatcagca tcctcacaga gaaaccttag
    74521 cccgtttgga agacagacaa attacttatt accgaacgga tacgcaagga gctattcgtt
    74581 tgactggttg gacaagctgg catcttgaaa cggtccgtta gttaaaattc cagcctgacg
    74641 aagaaacaag cagtcgcttt ccttttagga gaaagatgat ataataaaag catgattgcg
    74701 atagaaaaga ttgaaaaact gagtaaagaa aatttgggtc ttataaccct tgtcacagga
    74761 gatgacattg gtcagtatag ccagttgaaa tcccgcttaa tggagcagat tgcttttgat
    74821 aaggatgatt tggcctattc ttactttgat atgtctgagg ccgcttatca ggatgcagaa
    74881 atggatctag tgagcctacc cttctttgct gagcagaagg tggtcatttt tgaccatttg
    74941 ttagatatca cgaccaataa aaaaagtttc ttaaaagaaa aagacctaaa ggcctttgaa
    75001 gcctatttag aaaatccctt agagactact cgactaatta tctttgctcc aggtaaattg
    75061 gatagtaaga gacggcttgt taagcttttg aaacgtgatg cccttgtttt agaagccaac
    75121 cctctgaaag aagcagagct aagaacttat tttcaaaaat acagtcatca actgggtttg
    75181 ggatttgaga gtggcgcttt tgaacaactg cttttaaaat caaacgatga ttttagtcaa
    75241 atcatgaaaa acatggcttt tttaaaagcc tataaaaaaa cgggaaatat tagcctaact
    75301 gatattgagc aagccattcc taaaagttta caagacaata tttttgactt aaccagactt
    75361 gtcctaggag gtaaagttga tgtggctaga gatttgattc atgatttacg cttatcaggg
    75421 gaagatgata ttaaattaat tgctattatg ttggggcaat ttcgcttata tttacagctt
    75481 gccattcttg ctaaagaagg aaaatcagaa cagcagcttg taaccagttt gtcagagata
    75541 cttgatcggc gggttaatcc ttaccaggtc aagtatgcgt taaaggattc taggacctta
    75601 tctcttgcct ttctaacagg agcggtgaaa accttgattg agacagatta ccagataaaa
    75661 acaggacttt atgagaagag ttatctagtt gatattgctc tcttaaaaat catgactcac
    75721 tctcaaaaat aggcgttgga ccgctttcca agagataatc aaattagttg aaagctctta
    75781 ttttttgcga taagatagac gtatcataaa ggaaaagagg acaggaaaat ggctattatt
    75841 ttaccagaac ttccatacgc gtatgatgct cttgaaccac agtttgatgc ggagacaatg
    75901 actcttcatc atgataaaca ccatgccact tatgtcgcta ataccaatgc ggctttagaa
    75961 aaacacccag agatcggaga aaatctggag gaactattgg cagatgtcac taagattcca
    76021 gaagatattc gtcaagcctt aattaataat ggtggcggac acctaaacca tgcgcttttc
    76081 tgggaactat tatcacctga aaaacaagac gtcactcctg atgttgcaca agccattgat
    76141 gatgcttttg ggtcatttga cgcctttaaa gaacaattta cagcagctgc tactggacgt
    76201 tttggttcag gttgggcatg gttagttgtt aataaagaag gacaactcga aatcacatca
    76261 accgctaacc aagatactcc aatttcagaa ggcaaaaaac caattctagc gcttgatgtg
    76321 tgggagcatg cttactactt gaattatcgt aatgtacgtc caaattatat caaagctttc
    76381 tttgaaatca ttaattggaa aaaagtatca gaactttacc aagccgctaa ataagaaaag
    76441 ggtttccaat ctcaaaaaga gattggtttt ttaagtggtt ttgtgaataa cagtgtgggg
    76501 acatcatttt ttagaaaaga taaaaatagt ttgtcttttt aatgtaattc cttgattttt
    76561 agctaaattt agctagaata gaatggatag gagattttag gtgttaagaa agaagaaaac
    76621 taccattgta gggattattt taggaatgat gatagtgacc ttacttgtcg gctgtttgac
    76681 tggtaaaaag aaaggctttc ctcctaaagc aggtgagcgg cagcaaacgg ctgacttaaa
    76741 aatgaaaaaa gattagaaaa actgttcaaa aaccgttacc tgaaaaatct aaagaggcta
    76801 agcaagctgt tatggatgca gatttagcgc aaatggatgc cttagggctc tattacgatg
    76861 acaaccattt gagtttggtc aatacggtca aggtgtattt aaaggaattt ggcattggtg
    76921 aatctcaaat tgccttttcc tacaaagacc tcaattcagg caggactgct gccatgaatg
    76981 accatcagcc aatgattgca ggaagcacct ataagctgcc attaaacatg ctggtggtag
    77041 ataaggcaaa ttatccatga cagagcgttt tgacattaca gaaactgatt atgaatatga
    77101 tcaggaacat catgcctatg ttgctcaatt caatggtgcc atgtctattc cggatatgca
    77161 agaatattcc ttggtgtact cagaaaacac gccagcttat gctctagcag aacgattggg
    77221 tggcatgaat aaggcttacc aattatttga ccgctacggg aaggtatccg gagctattac
    77281 gaccattgat cgtaatggca ataaaatcac caccgcttac tatttgcaag gtttggatta
    77341 cttgtggcag catcaggata aatataagga tatcttgtac tacattggtg aatctttccc
    77401 tgatttgtat tacaagactt atttgcctca tgtaaaggtt tatcaaaaac cgggttatgt
    77461 cagagaagcc ttaaatgtag gtgccattgt ttgcgaagag tctccttatt tgattactct
    77521 ttattcatct ggtttaggtg gagcaacaca agcatcagag gaagtcaatg gccttggtta
    77581 tgtgcagctt gttcagctaa catatgtaat taatgagtgg catagagtca atcacaacat
    77641 gatctaatac tcagttgaca ttagtctgat agtagacata cttactgatg aaagtcacta
    77701 aaataagttt ttgctcatta aaaaagcttg gcctgtttct tcaggtcaag ctttttgaga
    77761 ttaatgctgt gtttttgagg tcttattgtc acctcttgtt tcttgataat ggtgaagcaa
    77821 ttttttgaat tttttaacgg cagcgcgtgt tagctgctta gatgatttgc catttctgct
    77881 gtaaatggga ttgtgcttat ccctttgctg ttctaaatcg ggaactagaa aagttgcatc
    77941 aggactatta attgcttttt caataaaagc cggatcaaaa agcagaatag ggtcaatatc
    78001 aagcagacgg tgcaagtcat cgttttggtt gccataattg aaagaagaac cattgacaat
    78061 actatttcgc gttaggttat caggatctag tgctactaaa aattgtccag attctgacaa
    78121 aataaactct gagtgaaaat gaattaaaat cttatagttg gcttgaggaa tattgtctgt
    78181 atagactgcc tgatgattgc cattagcatc taaaatacta gcaaccttat tatggtatct
    78241 agaagattca taaagagcat aatcgtcatc ttctagagta gccaaatatt tggctaaggc
    78301 atccgcatcg gttttccctt tattcccata atgtgagcga acccactccg tttgctgggt
    78361 tgatatgagg taacggagtt gatgaatttt ccgattaaaa cggctataag gaatgtgttc
    78421 gagattaaca taataagcct gaatcagggt agataattgg tcccaaaaag gatctgttgg
    78481 cgatagcttt ggacttaatt gtgccaatac ttgctgtgtt tgttttgatt gtcctgtcaa
    78541 ctcgtctggc atctttaaat acgtcaagag cagttgtaac cgcttttgag cttcttgagg
    78601 cgtctgtgcc atctcttctt gttgccaaag cttttgaaag tcaggtgatc ctatcaggcg
    78661 aatgcgacta taaagttgtt cgaaatgata agagtttcct ctaacagcga gttgttcaag
    78721 gtatcctttt tggatggctt tataggacca tccgtcctta tcgagctttc gaacataatc
    78781 aagcaagtcg tggcgtaatt taatagcttc tgaaagctgg agttctcgta atttaggata
    78841 gttttggtaa aaaggtgcca ctaaattttg ataaagtttg tgttcttttt caaaggcaat
    78901 ccgctgatag tctttctgcc aaaaactgac gcaaaaagtc agaaacatca agaagaatag
    78961 ccattttcca agtaatttca taggttttta gttgacaaac atggcatcgc caaagctaaa
    79021 gaaacgatac ttttcgtcaa ccgcgtgtct gtaagcttca aggacaaagt cacgccctgc
    79081 aaaggctgat actagcatca caagggttga ttttggaaga tggaaattag ttgaaaaagc
    79141 atcaactacc ttgaattggt agcctggttt aataaaaata ttggtccaac ctgaatcagc
    79201 ttgaatgtcc ccttggaact tacctccgat ggtttccaac gtacgaatag aggtggttcc
    79261 tacagcgaca acacgaccgc cagcttgttt gacgtctctt agggtttgcg ctgcttcttc
    79321 tgagagggaa taaaattccg aatgcatatc atgctcatcg aggttatcca cggaaacagg
    79381 tctaaaagtc ccaagcccta catgtagggt caaataaacc aaatgtaccc ctttggcttc
    79441 gatttttttg agcaagtctg ttgtgaagtg gagaccagca gttggggcgg ccgcagagcc
    79501 attttcttta gcataaacgg tttggtatcg ctcagcatcc tctaattttt cgtgaatata
    79561 aggaggcaat ggcatttcgc cgaggctctc taaaacttct aagaaaatcc catcatagct
    79621 gaattcaaca attcgtccac catgttctaa ttcgtcgata atggtggctt tgagacgccc
    79681 atctccaaaa ttgacttggc taccaacctt gagccgtttg gctggcttgg ctaaaacttc
    79741 ccattgatct ccttgtgtat ttttaagcaa aagcaattcc acatgaccat gagtatcggg
    79801 tttttctcca taaagtctgg caggcaagac acgggtgtta ttcatgacga gggcatcccc
    79861 tggatttaat tgatcaatga tatggtcaaa atggctatca accatagttt tttgacggtg
    79921 atcaataatc aatagttttg aactgtctct tttttcgagt ggggtttggg caatcaattc
    79981 ttctggtaac tcaaaatcaa aatcgttagt attcattggg actcctttaa agccactagt
    80041 aaatctcttc ctagtaactc tttcatgtaa gataaatctt ttctattata tcacttgtcg
    80101 tctaacccgt catctttaat ctttcatcag aaagtaaagg ataggcttga caaaaattgg
    80161 tatataccat ataataaaaa cacgaaaggt ctataccaat tagaaaagga gaatgacatg
    80221 aaaattattc gggtccaaga tcagattgaa ggtgggaaaa tcgcttttac cttattgaaa
    80281 gatagcttag ctaagggagc taagacgtta ggattagcaa caggaagttc accaattagt
    80341 ttttaccaag aaatggtcaa aagtcctttg gatttttcgg acttaacaag tattaacttg
    80401 gatgaatatg tcggtctttc agtagagagc gatcagagtt atgattattt tatgcgtcaa
    80461 aaccttttta atgcgaagcc ttttaaaaag aattacttgc ccaatggttt agcaactgat
    80521 atcgaagctg aagctaaacg atatgaccaa atcattgcag agcatccgat tgattttcaa
    80581 gtgcttggga ttgggcgtaa tgggcacatt ggttttaacg aacctgggac ctcttttgaa
    80641 gaagaaacgc atgtagtgga tcttcaggaa tcaactatca aagccaatag tcgtttcttt
    80701 acaagtattg aggatgtgcc aaaacaagct atctctatgg gaattgcctc catcatgaag
    80761 tccaaaatga ttgttcttct tgcctttgga caagaaaaag ctgatgcgat caagggaatg
    80821 gtttttggac caatcacaga agacctccca gcaagtattc tccaaaaaca tgatcatgtt
    80881 atcgtcattg tggatgaagc tgctgcgtca caattagatt aacctaacca gtcgttaaac
    80941 cttgtaagaa caaaaatgag gagatagtca acctcattta agaagaagtc ttgtaacttc
    81001 tatttctttt gcaagggtaa atttatgtta cactactaat atgcgtttag ataaattatt
    81061 agaaggtaca aaagttggct ctagaagtca agtgaagaaa ctgattaagg ctcaaggggt
    81121 ttgggttgat cacatgcctg ccagaaatgg tcgacaaaat gttgatcctg gcttgcaact
    81181 gattgaggtg actggacagc gagtgacgca tcctaaacat tcctacatta tcctcaacaa
    81241 gccatcaggg gtggtctcag ctaagaaaga tactaactat ttaaccgtta ttgatcagct
    81301 tgctgaagaa gataaaagtc cagacttgta tcctgttggc agattggata gagataccga
    81361 agggttggtt ttactcactg ataatggtcc cctagggttt cggatgttgc accctattca
    81421 tcatgtttct aaaacgtatc ttgtaaccgt taatggcttg ttagcagaag atgcctcaga
    81481 tttttttgca gctggtattt gtttcccaac aggtgaacaa tgtcaaccag ctcaattgac
    81541 tattcttaaa gccgacacag accagtcaca ggctagtttg accatttcag aaggaaaatt
    81601 tcaccaagtg aaaaaaatgt ttttgaccta tggtttgaaa gtgacctctc tgaaacggac
    81661 gcactttgca ggattggaac tcggtcattt agccagcgga gaatatcgtt atctaacagc
    81721 gtcagaaagg caactcatta aaacgtatct agactgagca aaacatgctt tcaaaaagaa
    81781 gctattcttg cttaatcaat aaagtgtcca ttgactatta tagtcatgat gagtccgact
    81841 tttgctatgt ttagtcatct tgttgttaca aaatcaggta agcaaaaaaa ttcaattccc
    81901 actgtctcct taacctgaag cgttataaaa acccttgtgt taaactctca taaattggaa
    81961 atgggagtgt tttttgatgg ctaaaaaaac taaagctact taaagtttgg tacttagttt
    82021 tcgaataata aggtggaaga tctaatagga ggtgacaaaa atattaaccg ctttagatgg
    82081 taagaatcag ctcatttcgc tagtaactca gcctatttca acgaaaccgc ctttttgttg
    82141 cccagcttgc aaatctcctg ttcgcttgcg tcaggggaca attagacgac ctcattttgc
    82201 ccatgtacaa ctagctcatt gtcaattcca agcagaaaat gaatctgagg aacatttgac
    82261 gttgaaggca aaactttaca ccagtttagt ccgaacagaa gctgtctgta ttgaaaaata
    82321 tcttcctgag ctgcaacaga tagcagacct ttgggtgaat gataagttag ccttagaaat
    82381 tcagtgcagt cccttgccag tggagcgtct aaagaaacgc accaaagcct atcaagaaaa
    82441 ggggtatccg gtacgatggt tactgggaag aaaattgtgg cttaacactc acttaactgc
    82501 tttgcaaaaa cagtttcttt acttttcatc tagtttgggc tttcatttgt gggagctgga
    82561 tgcagctgca aatctgctac gtctcaaata tttaatccat gaagacttat ttggtaaggt
    82621 aagttatcta accaaaacca tttctttaga ccacaatatt atggaaatgt ttcgattacc
    82681 ctatcagcaa gaaatccttt attcttatca gaaaaaaatg actgtgaacc tatctaaaag
    82741 aatacaaaga gctcttttag ctagacaccc gaaatggcta cgacgacaag aaaaggctta
    82801 cttatcaggc tataatttac ttatgttaac aactgatgct ttttatcctc aatggagacc
    82861 agttcaatct tccagcggtt tttgtcaaat taaaggaaat cttcgccctt attatgaaag
    82921 ttttaaagtc tattataaaa aagaaaagga taagaaggtg caaacgcttt tttcaccaaa
    82981 atattatgtt aaaatggata gtaatagaaa atgagaagga gatgttatga ctgataatcg
    83041 cagccattta gaagaaaaat acacttggga tttgagcact atttttgcaa cagataagga
    83101 ttgggaagct gaagtcagtg acttagctac tgaaattgac gcggctaagg atttttcagg
    83161 tcatttacta gattctagcg ctaatctgtt gacaattaca gatacttatt tagagttagc
    83221 tcgtcgtgtt gagaaagtct atgtttatgc tcacatgaaa aatgaccaag acactactgt
    83281 tgccaagtat caagaatacc aagctaaggc ttcaggcttg tatgcaaagt ttagcgaagt
    83341 gttttcattc tatgacccag aagttatggc attgagtcag gaagattacc aagcttttct
    83401 tgcggaaaca cctgatttaa aaaggtatca tcatttcttt gacaaattgt tccagacacg
    83461 tgaccatgtg ctcagccagg ctgaagagga attattagca ggagcccaag aaattttcaa
    83521 cggcgctgaa gaaaccttta gtattcttga taacgctgat attgtctttc cagtagttaa
    83581 aaatgataag ggggaagatg ttgaattaac ccatggtaac ttcattagcc taatggaatc
    83641 taaggaccgt agcattcgcc aagctgctta tgaagccatg tatagtactt atgaacagtt
    83701 ccaacatact tacgctaaaa ccttgcaaac caatgtgaaa gtgcaaaact ataaggcgcg
    83761 tgttcataaa tacgattcag cacgtcaagc tgccatggct gctaacttta ttccagaagc
    83821 tgtttacgat accttgttag aaacggttaa taaacatttg ccactcttac accgttattt
    83881 gaaattgcgt caagaggttc ttggtttaga cgatttgaag atgtatgatg tttacacacc
    83941 attatctgaa acagatttag ccattggtta tgatgaggct ttggaaaagg ctgagaaggt
    84001 tttggctgtt tttggtaagg attacgcgga tcgtgtacat cgtgctttta ccgaacgttg
    84061 gattgatgtt cacgtcaata aagggaagcg ctctggggct tattcaggtg gttcctatga
    84121 taccaatgcc ttcatgctgt tgaactggca agatacactt gataatcttt acacacttgt
    84181 ccacgaaaca ggccacagtt tgcattctac tttcactcgt gaaacacagc cttatgtgta
    84241 tggggattat agtatcttct tggcagaaat tgcatccaca acgaatgaaa acatcatgac
    84301 agaagctctc ttaaatgaag tgcaagatga gaaagaacgc tttgccattt taaaccatta
    84361 cctcgatggt ttccgtggaa cagttttccg tcaaacccaa tttgctgagt ttgaacatgc
    84421 cattcatcaa gctgatcaaa aaggagaagt gttaacaagt gaatacctta accagctata
    84481 cgctgattta aatgaaaaat attatggttt aagtaaaaaa gataatcatt ttattcagta
    84541 tgaatgggca cgtattcctc atttctatta caattattat gtttaccaat atgcaacagg
    84601 gtttgcagct gcaagctatt tagcggataa gattgtgcat ggcacacaag atgatatcga
    84661 tcattacttg gcctacctca agtcaggaaa ttctgattac ccacttgaag tgattgctaa
    84721 agcaggcgtt gatatggcaa aaggtgatta tttggaagct gcctttaaag tctttgacga
    84781 acgtctaaca gaacttgaag tactcgtgtc caaaggcata cacaaaagct aaaatcacaa
    84841 aagtcaagga caaaaagagt ttaggattga tagaaatgga gattcacagt tggtgtgaat
    84901 ctccatttct atcggtcatt ttctttttat attttagtgg attatcagtc ctgaaaagca
    84961 ggcttcattg accttagttt gacggtttct tttttgtctt ttttattgtg tggtcactca
    85021 cttaaataaa accaatagtt gagtaaggcg cttcagatta acgccattcc attgttttag
    85081 agataacgtt cgtataaaac gtgtttttat tatatttgac attttataaa agagataata
    85141 gaattaaaat actaacattt atgtttttta aaaaataaac gttcgtataa taaaaaagga
    85201 gttttttatg gaaaaaacaa aacgttatat cattgcaaca gcagggatac ttctccactt
    85261 aatgttggga tcgacctatg cttggagtgt ttatcgtaat ccaattttac aagagaccgg
    85321 ttgggatcaa gctcctgtcg cttttgcctt ttctttagct attttttgtt taggtttatc
    85381 tgctgctttt atgggaaatt tagttgaaca atatggtcct cgtctaaccg gaacggtgtc
    85441 ggctattctt tatgcttctg gaaatatgct gactggttta gcgattgatc gtaaagaaat
    85501 ttggctttta tatattggtt atggtgttat tggtggctta ggacttggag cgggatatat
    85561 cacaccgata tcaactatta taaaatggtt tccagataaa agaggcatgg caacagggtt
    85621 tgctattatg gggtttggtt ttgcgtcttt attaacaagt ccgattgccc agtggctaat
    85681 tgaaacagaa ggtttggtag caacatttta ccttttaggg ctgatttatc taatagttat
    85741 gctttttgct tctcaattga taataaaacc aactgctgct gagatagcta tactagataa
    85801 aaaaagatta caaaataaca gttatttaat cgaaggaatg accgctaaag aagcacttaa
    85861 aacaaaatca ttttattgtc tttgggtaat tttatttatc aatatcacat gtggcttagg
    85921 acttatctca gttgttgcgc caatggcaca agatctaact ggtatgagtc cagaaatgtc
    85981 agcaattgtg gttggtgcta tggggatttt taacggtttc ggtcgtttag tgtgggccag
    86041 tttgtcagac tatattggac gccgtgtgac agtaatactt ttatttttag taagcataat
    86101 tatgactatt agtttaatat ttgcacatag ttccctaatt ttcatgataa gcattgccac
    86161 ccttatgact tgttatggtg cgggtttttc tctcattccg ccttatttaa gtgatctttt
    86221 tggggcaaag gaactggcta cattacatgg gtatatattg acagcttggg caatagctgc
    86281 attaactgga cctatgttat tatctatcac tgttgaatgg actcataatt atcttcttac
    86341 cttatgtgtt tttattgtct tatacatttt aggattaatg gtagcattaa ggttaaagaa
    86401 atgatgtcct ataaaaaatt taaaagtaaa ccctagcaaa gcctagggtt tttttatata
    86461 attaggttta tggttaaatc atacagtaaa acagcaaatc ataatatgcg tcgtcctgtt
    86521 gtcaaagaag agcttgttca ttatatgcga acacggcaga agcaaatcac aggtttttta
    86581 gcagagttag agcaattcgc aagacaagaa aacattccta ttattcagcc tgaggtcgtg
    86641 gcttatttca ggtttttatt gcaaagctta cagccaaaac acattttgga gattggcaca
    86701 gccattggtt tttctgcgct tttaatggct gaaaatgcac cagatgcgac gattgtgact
    86761 attgatcgta atcgagagat gatcgatttt gctaaagcaa attttgctaa gtacgacagt
    86821 cgccaacaga tccgcctgtt agaaggagat gcagcagata tattatctac acttgaagga
    86881 aattttgact ttgtgtttat ggattccgcc aaatccaaat atattgtctt tctaccagag
    86941 attttaagac tgttaaaagt cggtggtgta gtcattttgg atgatgtctt tcaaggaggt
    87001 gacattacaa aaccgatcga agacattcga cgaggtcagc gtactattta tcgtggctta
    87061 cagtctttgt ttgatgctac cttaactcat ccaaacctaa caactagcct ggtccctctt
    87121 agtgatggat tattaatgat tcgtaaaaat caagcagata ttctattacc agattaagta
    87181 acattgataa atttatgtta taataaatta gttaaagata aaaaggagct cagaagtaaa
    87241 tgaaaaactc aaataaactc attgctagtg ttgtgacatt ggcctcagtg atggctttag
    87301 cagcttgtca atcaactaat gacaatacta aggttatttc gatgaaaggt gatacaatta
    87361 gcgttagtga tttttacaat gaaacaaaga acacagaagt atcgcaaaaa gcgatgctaa
    87421 atctggtaat tagtcgtgtt tttgaagctc aatatggtga taaggtttca aaaaaagaag
    87481 ttgaaaaggc gtatcataaa acagctgaac agtatggcgc ttcattctct gctgctttgg
    87541 cacaatcaag cttgacacct gagactttta agcgtcagat ccgctcttca aaattagtag
    87601 aatatgcggt taaagaagca gctaaaaaag aattgacaac acaagaatat aagaaagcat
    87661 atgaatctta tactccaaca atggcagtcg aaatgattac tttagataat gaagagacag
    87721 ctaaatcagt cttagaggaa ctaaaagccg aaggcgcaga ctttacagct attgctaaag
    87781 aaaaaacaac aacacctgag aaaaaagtga cctataaatt tgattcaggc gcgacaaatg
    87841 taccgactga tgtcgtaaaa gcggcttcaa gtttgaatga gggtggcata tcagacgtta
    87901 tctcggtttt agatccaact tcttatcaaa agaagtttta cattgttaag gtgactaaaa
    87961 aagcagaaaa aaaatcagat tggcaagaat ataagaaacg tttgaaagct atcattatag
    88021 ctgaaaaatc aaaagatatg aatttccaaa acaaggttat tgcaaatgca ttggataaag
    88081 ctaatgtaaa aattaaagac aaagcttttg ctaatatttt ggcgcaatat gcaaatcttg
    88141 gtcaaaaaac taaagctgca agtgaaagtt caacaaccag cgaatcatca aaagctgcag
    88201 aagagaaccc atcagaatca gagcaaacac agacatcatc agctgaagaa ccaactgaga
    88261 ctgaggctca gacgcaagag ccagctgcac aataagtagg attttattga ctaatcagtt
    88321 aaaaaaactt ataataattt agttaagaat tgtttatcaa ggattagata cagaaaagtg
    88381 acggttgctg cgagtcattg ataattagat aaacatgcta cttaacttta caacaattga
    88441 tatcgtaaat taagaatgac aagttcattg aatagaggtg gtaccgcggt tttcgccctc
    88501 tgtgagatgg acttgttttg tattgaggac tatttgaaat caacgaaagg aatagagtag
    88561 actaggctgc caggcttata gctgatctcc tagcaagaaa gacgagctgt gtcaactggt
    88621 gtgattacat tactagcttc agcataacac tgtttttctt actctttgta tctaaatttt
    88681 tatgaaagaa ttatcgtctg cacaaatccg ccaaatgtgg ttggatttct ggaaatctaa
    88741 aggacattgc gttgagcctt cagctaactt ggttcctgtg aatgacccaa cgcttctttg
    88801 gatcaactca ggtgttgcaa ccttgaaaaa atactttgat ggttcagtga ttccagaaaa
    88861 tccacgtatt accaatgcac aaaaatcaat tcgtactaat gatattgaaa atgttggtaa
    88921 aacagcacgt caccatacta tgtttgaaat gcttggtaac ttctcaattg gagactattt
    88981 ccgtgatgaa gctattgagt ggggatttga actcttgaca agtccagact ggtttgattt
    89041 ccctaaagat aagctctaca tgacttatta cccagatgac aaggattcat ataaccgttg
    89101 gattgcttgt ggcgttgaac caagtcactt ggtgccgatc gaggataact tctgggaaat
    89161 cggtgctggt ccttcaggtc cagatacgga gattttcttc gatcgtggtg aagatttcga
    89221 tccagaaaat atcggacttc gcctcttggc tgaagatatc gaaaacgatc gttacatcga
    89281 aatctggaac atcgttctct cacaattcaa tgctgaccca gccgtaccac gttcagaata
    89341 caaagaatta ccaaacaaaa acattgatac aggtgctggt cttgaacgtc ttgcagctgt
    89401 tatgcaaggg gcaaaaacaa actttgaaac tgacctcttc atgccaatca tccgtgaagt
    89461 agagaagttg tcaggtaaaa cttataatcc agatggcgac aacatgagtt tcaaggttat
    89521 cgctgaccac atccgtgcgc tttcatttgc tatcggtgat ggtgcgcttc ctggaaatga
    89581 aggtcgtggt tacgttcttc gtcgtcttct ccgtcgtgct gttatgcatg gtcgtcgtct
    89641 tggcatcaac gaaactttcc tttacaaatt ggttccgact gttggacaaa tcatggaaag
    89701 ctactaccca gaagtgcttg aaaaacgtga ttttatcgag aaaatcgtta aacgtgagga
    89761 agaaacattt gctcgtacta tcgatgcagg tagcggtcac ttagattcat tgcttgcgca
    89821 gcttaaggct gaaggtaagg atactcttga aggtaaagat atcttcaaac tttatgatac
    89881 ttatggattc ccggttgaat tgacagagga attggcagaa gatgcaggct acaagattga
    89941 tcacgaaggc tttaagtcag ccatgaaaga acaacaagac cgtgcgcgtg cagctgttgt
    90001 taagggtggt tcaatgggga tgcaaaatga aaccctagct ggtattgttg aagaatcacg
    90061 attcgaatac gacacatata gtcttgaatc aagtctttca gtcatcatcg ctgataatga
    90121 acgtaccgaa gctgtttcag aaggtcaagc tcttcttgtc tttgctcaaa caccattcta
    90181 tgctgaaatg ggtggacagg ttgctgacac aggtagaatc aaaaatgata agggtgacac
    90241 agttgctgag gttgttgatg ttcaaaaagc accaaatggt caacctctac acactgtaaa
    90301 cgttttggca tcactttcag ttggaacaaa ctacacactt gaaatcaaca aagagcgtcg
    90361 tttggctgtt gagaaaaacc acacagcaac tcacttgctt catgcagccc ttcacaatgt
    90421 tatcggtgaa cacgcaactc aggctggttc attgaacgaa gaagaattct tgcgctttga
    90481 ttttactcac tttgaagcag taagcaatga ggaacttcgt cacattgaac aagaagttaa
    90541 tgagcaaatt tggaacgctc ttacgatcac aacgactgaa actgacgttg aaaccgcaaa
    90601 agagatggga gcaatggcac tttttggtga gaaatatggt aaagtggttc gtgtggttca
    90661 aattggtaat tattctgttg aactttgtgg tggaactcac ttaaataatt cttcagaaat
    90721 cggtctcttc aagattgtca aagaagaagg tattggttca ggcactcgtc gtattattgc
    90781 agttactggt agacaagctt ttgaagctta tcgtaaccaa gaggatgccc taaaagagat
    90841 cgctgctact gtaaaagctc cgcaattgaa agatgcagca gctaaagtac aagctcttag
    90901 cgactcactt cgtgatcttc aaaaagaaaa tgcagagctt aaagaaaaag cagcagctgc
    90961 agcagctggt gatgtcttta aagatgttca agaagctaag ggcgtgcgct tcattgctag
    91021 tcaagttgat gttgcagatg caggggcact tcgtacattt gctgataact ggaaacaaaa
    91081 agactactct gatgtgcttg ttctcgtagc agctattggt gagaaggtta atgtccttgt
    91141 tgcaagcaaa actaaagatg tccacgctgg taacatgatc aaagaattgg caccaattgt
    91201 agcaggtcgt ggtggaggta aaccagacat ggctatggca ggtggtagcg atgcaagtaa
    91261 aattgcagag ctgctagcag cagttgctga aacagtgtaa atccataaaa aaccaagtta
    91321 tttagaactt ggttttttat atttgaaatg actaccatca taaccttttt gactattatc
    91381 gttgtcaaaa agaaaaagat atgttacaat tatagtaaca aatagacatg aggaggtgtt
    91441 agagaaaatg acacatggta tcaaacttaa gaggcagatt tgcttatggg caagtatcct
    91501 gactcttttc attttaagtt atcatcttat cagggtaatc tggtttggag tacccttgag
    91561 ttatctggtt ttgggcttta caggactagc cttgctgttg aatacttttt cttattttag
    91621 aaagtaagtt ataagttaaa gaagaatgaa ggagatggca atggaggtta ttcttaaaaa
    91681 tcggctcaaa gaattacgag ccagagatgg aattaatcaa acggaaatgg ctaaactggc
    91741 aggagtatcg aggcagacca tcagtctcat tgagcgtaac gagtacaccc catcagttat
    91801 aattgccatg aaaattgcaa aggtatttca ggaaccagtt gaagaggtgt ttcgtctagt
    91861 ggaggtcgag gaatgaaaaa gaagaagaat agttttttac gcttgttaaa aatgttattg
    91921 ctatcaagtt tggcgggagg gattataggt ggcatggttg gagccttttt aggttaccat
    91981 ggagagagat tagatcagct cacgttccta aaagatgatg tgattaacct gattatctta
    92041 ttaaatcgtc tagtagttgt cacaggcctc acactttcct ttgttttttt aacgcaactg
    92101 aaaaaagaga cagctgttta taacacaatt gaagaggatg attattctga aaatggttac
    92161 cgtcagctga ataaaaagca tgcttatacc atgttattga ttgccgtagc ttctatttta
    92221 tccatgtgca atgtgctact tggtctgacc ttaactaatg attcacagca tgccatgctg
    92281 gccattccgc ttctggatat ccttttatta ttaatggtca ttccttttca agctttagcc
    92341 atgaaacgtt acaatgccat tcggggaacc gatgttcctt attttccaaa tttgaaagaa
    92401 ttaaaacata atatcatggc tctagatgag gcagagttac aagcctatca taagacatcg
    92461 tttgaaagtg tgttgtcatt aaatggagtc atcatccctt ccttgtatgt gattttattt
    92521 tttgtctatc tattcactgg tcaagtggag ttaacagcca tcttagtttt ggtgttaatt
    92581 cagctctatt tactcgttaa gagtgcaaca atgaccagac aattttaccg ttagcctaaa
    92641 aggagactgt ttatgaaagg attcattaat tatttaaaaa tagcagtgct cattattctg
    92701 gctatggttt tcaatgtcct tccgatgatt ttattgcaaa agcaacacga tattcctatg
    92761 gtgcttaatt ggggaattgg tattttctac ttggttattg ttggaagtgt ccttattgta
    92821 ttatggcgtc tttatcaagc taagcaagac acccctatta aacagcaaaa aatgagattg
    92881 gttgactggg gttatttagc attattttgg ttaatcatcc gtgtgatagc tattgcaggt
    92941 acccttgtta accagctatg gtctggtcaa caagtgagtg ctaatgatgc tgcaatacat
    93001 accttagcta gacttatcaa aggtggtttc ccgctttata ctgccctatt tgtacttgtg
    93061 atagctttta tcgctcctat tatggaagaa ctagtcttta gaggatttcc tatgattgat
    93121 ctcttcaaag gaaaatcact taaggtggca ggtttagtga cctctcttgt ttttgcttta
    93181 ccacatgcca ccaatagtgt tgaatttatc atgtacagct gtatgggcat ttttctcttt
    93241 gttgcctatc aaagacgagg aaacttaaaa gacgctatct tgttacatat ttttaataac
    93301 ttgattgaag tcattttgtt aatgtcaata ggcttaggag tcatataata aaaagcttcg
    93361 gatggatgaa ctgcacccca aaagttggac aaaaaaatct aacatttggg ttggaattgt
    93421 caagaaaaaa tagacataaa gttaagctaa tgagtaacta tttttttcaa attcaacagg
    93481 tgagagatac cctaaagctg aatgcagacg tttggggtta tagtaagtct cactatattt
    93541 aaaaatctca agcttagctt gctcaatagt tgcgaaatga gcatcattga caagctctct
    93601 ttttaatgtt ttataaaagg attccatgag tgcattatcg tatggattac cttttcgact
    93661 catcctggac ttacattttt tccatctaag taggtcctga aatcgtgctc cggtatattg
    93721 agaaccctga tctgtatgga cgattacccc ttctttaggc ttttctctat tataagcttg
    93781 attgaaagct tctgttacca acttgtcttg catacgttgg cccattgccc aaccaacaac
    93841 ctttctacta taaacgtcta tcaagacaga cacataaagt attccttctt gtgtaggaat
    93901 gtaggttagg tctcctagcc ataatttatt cttaccagtc gcttggaaac attgattaac
    93961 aagattggga cgagttcatg aagaacgtcg acggttatag tatttatatt gatatcgact
    94021 tcctttagca tagag
//
LOCUS       DATXQK010000002        89795 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.002, whole genome shotgun sequence.
ACCESSION   DATXQK010000002 DATXQK010000000
VERSION     DATXQK010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 89795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 89795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..89795
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.002"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            517..1044
                     /locus_tag="VC394_000089"
     CDS             517..1044
                     /locus_tag="VC394_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEP5993592.1"
                     /translation="MSIDDYRPTYMVEAIYDLRANDLLRHGITAVLVDLDNTLIAWNN
                     PDGTPEVRAWLDEMTIADISVVVVSNNKHSRVERAVSRFGVDFISRALKPFAYGIEKA
                     IARYGFDRNEVIMVGDQLMTDIRASHRAGIKSVLVKPLVASDAWNTKINRWRERRVMA
                     KLEEKYGKLSYQKGI"
     gene            1044..2162
                     /gene="yqeH"
                     /locus_tag="VC394_000090"
     CDS             1044..2162
                     /gene="yqeH"
                     /locus_tag="VC394_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEP5993593.1"
                     /translation="MEELFCIGCGIQIQTEDKEKAGFTPAAALKKGMETGELYCQRCF
                     RLRHYNEITDVHITDDEFLRLLHEVGDSDALVVNVIDIFDFNGSIIPGLSRFISGNDV
                     LLVGNKKDILPKSVKDGKVTQWLTERAHEEGLRPLDVMLTSAQNKYAIKDLIERINEL
                     RNGRDVYVVGVTNVGKSTLINAIIQEITGDKDVITTSRFPGTTLDKIEIPLDDGSFIF
                     DTPGIIHRHQMAHYLSPKELKIVSPKKEIKPKTYQLNPEQTLFLGGLSRFDFINGERQ
                     GFTAFFDNQLELHRTKLAGADAFYDKHVGTLLTPPGKKELTAFPKLVRHEFTIDQKMD
                     IVFSGLGWIRVNGQKDSKAIVAAWAPEGVAVIVRKAII"
     gene            2188..2496
                     /gene="yhbY"
                     /locus_tag="VC394_000091"
     CDS             2188..2496
                     /gene="yhbY"
                     /locus_tag="VC394_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEP5993594.1"
                     /translation="MLTSKQRAFLKSEAHSLKPIVQIGKNGLNDHIKTSIRQALDARE
                     LIKVTLLQNTDEDIHEVAEILEEEIGCDTVLKIGRILILYKVSAKKENRKLSPKVKAI
                     "
     gene            2565..3197
                     /locus_tag="VC394_000092"
     CDS             2565..3197
                     /locus_tag="VC394_000092"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEP5993595.1"
                     /translation="MALELLTPFTKVELEEEKKESNRKQIGILGGNFNPIHNAHLVVA
                     DQVRQQLGLDQVLLMPECKPPHVDAKETIDEKHRLRMLELAIEDVEGLAIETCELERQ
                     GISYTYDTMLYLTEQHPDVDFYFIIGADMVDYLPKWHRIDELVKLVQFVGVQRPKYKA
                     GTSYPVIWVDLPLMDISSSMIRDFIKKGRQPNYLLPKRVLDYITQEGLYQ"
     gene            3194..3787
                     /gene="yqeK"
                     /locus_tag="VC394_000093"
     CDS             3194..3787
                     /gene="yqeK"
                     /locus_tag="VC394_000093"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEP5993596.1"
                     /translation="MTYEDYLPYSRTELLAKIAEQMSPKRFKHVLGVEKAALSLAECY
                     GCNPDKAGLAALLHDYAKECPDQVFLDLIDKYQLSPELAKWNNNVWHGMVGIYKIQED
                     LGLKDKDILRAIEIHTVGAAEMTLLDKVLYVADYIEEGRIFPLVDDARKIAKLDLNQA
                     VAYETVNTVAYLASKAQPIFPQTLDTYNAFCSYLKEY"
     gene            3811..4164
                     /gene="rsfS"
                     /locus_tag="VC394_000094"
     CDS             3811..4164
                     /gene="rsfS"
                     /locus_tag="VC394_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEP5993597.1"
                     /translation="MKKEELLKIVVEATDEKRAKDILALDLEGLTSLTDYFVIASATN
                     SRQLEAIADNIREKVKEAGGDASHVEGNSQAGWVLLDLTDVVVHLFLEDERYHYNLEK
                     LWYEAPAVALDAYLA"
     gene            4212..4955
                     /locus_tag="VC394_000095"
     CDS             4212..4955
                     /locus_tag="VC394_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEP5993598.1"
                     /translation="MKHNYEKFASVYDAVMDDSLYDLWTDFSLRHLPKSKGRNRLLEL
                     ACGTGIQSVRFAQAGFDVTGLDLSQDMLAIAKKRAQSAKKKIDFIQGNMLDLSQVGQF
                     DFVTCYSDSICYMQDEVDVGDVFKEVYDVLANDGIFIFDVHSTYQTDECFPGYSYHEN
                     ADDFAMVWDTYADEAPHSVVHELTFFIQEDDGRFSRFDEVHEERTYELLTYDILLEQA
                     GFKSFKVYADFEDKEPTKTSKRWFFVAYK"
     gene            5209..6315
                     /locus_tag="VC394_000096"
     CDS             5209..6315
                     /locus_tag="VC394_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEP5993599.1"
                     /translation="MTVTGIIAEFNPFHNGHKYLLETAEGLKIIAMSGNFMQRGEPAL
                     IDKWIRSEMALKNGADIVVELPFFVSVQSADYFAQGAIDILCQLGIQQLAFGTEDVID
                     YQKLIKVYEKKSKQMTAYLSTLEDTLSYPQKTQKMWEIFAGVKFSGQTPNHILGLSYA
                     KASAGKHIQLCPIKRQGAAYHSKDKNHLLASASAIRQHLNDWDFISHSVPNAGLLINN
                     PHMSWDHYFSFLKYQILNHSDLTSIFQVNDELASRIKKAIKVSQNIDHLVDTVATKRY
                     TKARVRRILTYILVNAKEPTLPKGIHILGFTSKGQAHLKKLKKSRPLITRIGAETWDE
                     MTQKADSIYQLGHQDIPEQSFGRIPIIIKNERLN"
     gene            6797..7513
                     /locus_tag="VC394_000097"
     CDS             6797..7513
                     /locus_tag="VC394_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEP5993600.1"
                     /translation="MGRKWANIVAKKTAKDGATSKVYAKFGVEIYVAAKQGEPDPELN
                     TALKFVIDRAKQAQVPKHVIDKAIDKAKGNTDETFVEGRYEGFGPNGSMIIVDTLTSN
                     VNRTAANVRTAYGKNGGNMGASGSVSYLFDKKGVIVFAGDDADSVFEQLLEADVDVDD
                     VEAEEGTITVYTAPTDLHKGIQALRDNGVEEFQVTELEMIPQSEVVLEGDDLETFEKL
                     IDALESDDDVQKVYHNVADF"
     gene            7716..8558
                     /locus_tag="VC394_000098"
     CDS             7716..8558
                     /locus_tag="VC394_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEP5993601.1"
                     /translation="MIKKQYLGRATIALASTLVLAACGSSKTAESGNQGSSKEVLFAT
                     VGTTAPFSYEKGGQLTGYDIEVAKAVFKGSDDYRVSFKKTEWSSIFTGLDSGKYQMGG
                     NNISFTKERSAKYLFSYPIGSTPSVLVVPKDSDIKSFDDIQGHTTQVVQGTTSVAQLE
                     DFNKKHSDNPVTLKFTNENITQMLTNLSEGKADFKIFDAPTVNAIIKNQGLDNLKTIE
                     LTSTEQPFIYFIFSQDQEKLQSFVNKRIKELTADGTLSKLAKEHLGGDYVPSDKELKL
                     PTAN"
     gene            8885..9730
                     /locus_tag="VC394_000099"
     CDS             8885..9730
                     /locus_tag="VC394_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEP5993602.1"
                     /translation="MSFKKWIGIVGIALASTVLVACSEKQDDKNTLTIGVMTKTESDQ
                     ARWDKVEELLKKDNITLKYKEFTDYSQPNKAVANGEVDINAFQHYNFLNNWNKENKEH
                     LVAIADTYISPINLFSGTSQDGKAKYKSVADLPNGAQIAVPNDATNESRALYVLQSAG
                     LIKLNVSGDQLATIANISENKKKLDIKELDASQTARALVSADAAVVNNSYAVPAKIDY
                     KTSLFKEKADDNSKQWINIIAGQKDWEKSEKADAIKKLIKAYQTDEVKKVVEKTSNGI
                     DVSVW"
     gene            9980..11044
                     /locus_tag="VC394_000100"
     CDS             9980..11044
                     /locus_tag="VC394_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEP5993603.1"
                     /translation="MNEAIIQLDHIDITFRQKKRVIEAVKDVTVHINQGDIYGIVGYS
                     GAGKSTLVRVINLLQAPTNGKITVDGDVTFDQGKIQLSADALRQKRRDIGMIFQHFNL
                     MAQKTAKENVAFALRHSSLSKTEKEHKVIELLELVGLSERADNYPAQLSGGQKQRVAI
                     ARALANDPKILISDEATSALDPKTTKQILALLQELNRKLGLTIVMITHEMQIVKDICN
                     RVAVMQNGVLIEEGSVLDIFSNPKEALTQEFITTATGIDEALEKINQQDIVKHLPANA
                     LLAQLKYAGTSTDEPLLNSIYRQFEVTANILYGNIEILDHIPVGDMIVVLEGQAENIL
                     AAEKALHEAGVDVSILKRGA"
     gene            11045..11737
                     /locus_tag="VC394_000101"
     CDS             11045..11737
                     /locus_tag="VC394_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP5993604.1"
                     /translation="MSQLIQTYLPNVYELGWSGDAGWGLAIWNTLYMTIVPFIVGGAI
                     GLLLGLLLVLTGPDGVIENKTICWVIDKVTSSFRAIPFVILIAILASFTYLLLRTTLG
                     ATAALVPLTFATFPFYARQVQVVFSELDKGVIEAAQASGATFWDIVKVYLSEGLPDLI
                     RVSTVTLISLVGETAMAGAIGAGGLGNVAISYGYNRFNNDVTWVATIIILLIIFAIQF
                     IGDSLTRRFSHK"
     gene            complement(11791..13161)
                     /gene="brnQ"
                     /locus_tag="VC394_000102"
     CDS             complement(11791..13161)
                     /gene="brnQ"
                     /locus_tag="VC394_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEP5993605.1"
                     /translation="MKQKNVYIVIGFMLFALFFGAANLIYPAFLGIYSGHQILWSIIG
                     FCLTGVSLPLLGVIAVAKSGSGDVESLARPISKWYAIFYSSILYLSIGPFFAIPRTGA
                     TSFSVGIAPILGDNTTNKAIYAILFFGLSYFLAIKPSKLAENIGKFLTPTLLVVISIL
                     VIASFVHPAGNYGDAFNAGVGVNNAFKDFPFIAGLIQGYGTMDALASLVFAILVIEAT
                     KQFGAKTDKEVTKITLISGAIAILLLALVYIFVGRIGATSQSLFPFSDGSFTLHGNPV
                     NGGQILSHASRFYLGGIGQAFLAVVIFLACLTTSTGLITSSAEYFHKLVPALSHIAWA
                     TIFTLLSAFFYFGGLSVIINWSAPVLFLLYPLTVDLIFLVLAQKCFNNDPIVYRTTIG
                     LTFIPAIFDALLTLSQMTGLFHLPEAVVTFFQKTVPLGQFSMGWIIFAAIGFLIGLIL
                     SKTKKS"
     gene            13395..14609
                     /gene="sstT"
                     /locus_tag="VC394_000103"
     CDS             13395..14609
                     /gene="sstT"
                     /locus_tag="VC394_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012516260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEP5993606.1"
                     /translation="MKKIYDLWVRVSLIKKIGIGVVIGVMLGILAPDLTGFSILGKLF
                     VGGLKAIAPLLVFALVSQAISHQKKGKQTNMTLIIVLYLFGTFASALVAVLTAYLFPL
                     TLVLNTPVNTELSPPQGVAEVFQSLLLKLVDNPINALATANYIGVLSWAIIFGLALKA
                     ASQETKHLIKTAAEVTSQIVVWIINLAPIGIMSLVFTTISENGVGILSDYAFLILVLV
                     GTMVFVALVVNPLIAVLITRQNPYPLVLRCLRESGLTAFFTRSSAANIPVNMQLCQKI
                     GLSKDTYSVSIPLGATINMGGAAITINVLTLAAVHTFGIPIDFLTALLLSVVAAVSAC
                     GASGVAGGSLLLIPVACSLFGISNDLAMQVVGVGFIVGVIQDSCETALNSSTDVLFTA
                     IAENAFWKRKKA"
     gene            complement(14664..15338)
                     /locus_tag="VC394_000104"
     CDS             complement(14664..15338)
                     /locus_tag="VC394_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEP5993607.1"
                     /translation="MLKRKTVGVLGLGIFGRTVARELSNFDQDVIAIDIRESHVKEVA
                     DLVTKAAVGDITDKEFLLAVGIEHCDTVVIASGNNLESSVLAVMHCKKLGVPTIIAKA
                     KNKIFEEVLYGIGATKVITPERDSGKRVASNLLRRHIESIIYLEHGISMIEFVIPKSW
                     EGQSLSELDVRRKYELNVIGMRQKEVKTLDTNVKPFEPLEPNTIIVAIANDHTFEKFD
                     YLGYLK"
     gene            complement(15348..16739)
                     /locus_tag="VC394_000105"
     CDS             complement(15348..16739)
                     /locus_tag="VC394_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEP5993608.1"
                     /translation="MSGGNMKRSFIKSLSVTQRLTFSFAIVILIGTLLLSMPFTHYQN
                     GPNTVYLDHFFNVVSMVCVTGLSVVPVAEVYNGIGQTIAMALMQIGGLGLVTLIAVST
                     FALKRKMRLSDQTLLQSALNRGDSKDLKHYLFFAYKVTFSIEAFAAIVIMIDFIPRFG
                     WKNGIFNSIFLAVSAFCNAGFDNLGSSSLKDFMLNPTLNVIITFLIISGGLGFAVWVD
                     LGVAFKKYFFERPHCYGATFRKLSNQSRLVLQTTAVILFLGTFLTWFLEKDNSKTIAN
                     FSLHQQLMVSFFQTVTMRTAGFATISYNDTLAPTNILYMIQMVIGGAPGGTAGGIKVT
                     TAAITFLLFKAELSGQSEVTFRNRIIANKTIKQTMTVLIFFFAVLMIGFILLLSVEPH
                     IAPIPLLFESISAIATVGVSMDLTPQLSTAGRLIVIVLMFVGRVGPITVLISLIQRKE
                     KTIQYATTDILVG"
     gene            complement(16809..17522)
                     /gene="rsmG"
                     /locus_tag="VC394_000106"
     CDS             complement(16809..17522)
                     /gene="rsmG"
                     /locus_tag="VC394_000106"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEP5993609.1"
                     /translation="MTPQDFYRTLEEDGFSLSSKQKEQFDTYFKLLVEWNTKINLTAI
                     TEENEVYLKHFYDSIAPILQGFLANEPIKLLDIGAGAGFPSLPMKILFPNLEVTIIDS
                     LNKRISFLTLLAQELGLENVHFFHGRAEDFGQDKAFRGQFDVVTARAVARMQVLSELT
                     IPFLKIGGKLIALKAQAADQELEEAKNALCLLFGKVIKNHSYQLPNGDSRFITIVEKK
                     KETPNKYPRKAGLPNKKPL"
     gene            17672..18229
                     /locus_tag="VC394_000107"
     CDS             17672..18229
                     /locus_tag="VC394_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEP5993610.1"
                     /translation="MPTVLIILVVLGVLALWLMISYNSLVKSRMHTKEAWSQIDVQLK
                     RRNDLIPNLIETVKGYASYEQKTFEKITDLRARVANASTPQETMAASNELSKQVTSLF
                     AVAENYPDLKANENFLKLQEELTNTENKISYSRQLYNSTTSNYNLQLESFPSNIAGKL
                     FGFKPSEFLQTPEAEKEVPKVEFNF"
     gene            18276..19172
                     /gene="htpX"
                     /locus_tag="VC394_000108"
     CDS             18276..19172
                     /gene="htpX"
                     /locus_tag="VC394_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEP5993611.1"
                     /translation="MLYQQISQNKQRTVVLLVVFFALLALIGASAGYLLLDNYAMGLV
                     LALVIGVIYATSMIFQSTSLVMSMNNAREVTEKEAPGFFHIVEDMAMVAQIPMPRVFI
                     IEDPSLNAFATGSSPQNAAVAATTGLLEVMNREELEGVIGHEISHIRNYDIRISTIAV
                     ALASAVTVISSIGGRMLWYGGGSRRQRDDGDDDVLRIITLLLSLLSLLLAPLVASLIQ
                     LAISRQREYLADASSVELTRNPQGMINALEKLQLSQPMKHPVDDASAALYINEPRKKR
                     SFSSLFSTHPPIEERIERLKNM"
     gene            19406..19939
                     /locus_tag="VC394_000109"
     CDS             19406..19939
                     /locus_tag="VC394_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEP5993612.1"
                     /translation="MLAISEIRKHPDGLSFDRLCDVKSMLLERDQQIIDIKAVKAVGN
                     VRYDKGLYLLDYQLSYEVILPSSRSMVPVCLSEVQHIQELFIEATDLADKKELVEDNL
                     VLVLDKDAINLEESIVDNILLAIPLQVLTEEEKKSKELPAGQNWAVLTEEDYQCLKEE
                     KQKENNPFASLQGLFDE"
     gene            20206..20892
                     /gene="covR"
                     /locus_tag="VC394_000110"
                     /gene_synonym="csrR"
     CDS             20206..20892
                     /gene="covR"
                     /locus_tag="VC394_000110"
                     /gene_synonym="csrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CovR"
                     /protein_id="HEP5993613.1"
                     /translation="MTKKILIIEDEKNLARFVSLELQHEGYEVIVEVNGREGLETALE
                     KEFDLILLDLMLPEMDGFEVTRRLQTEKTTYIMMMTARDSIMDVVAGLDRGADDYIVK
                     PFAIEELLARIHAIFRRQDIESEKKVPSQGIYRDLVLNPQNRSVNRGDDEISLTKREY
                     DLLNILMTNMNRVMTREELLSNVWKYDEAVETNVVDVYIRYLRGKIDIPGKESYIQTV
                     RGMGYVIREK"
     gene            20898..22400
                     /gene="covS"
                     /locus_tag="VC394_000111"
                     /gene_synonym="csrS"
     CDS             20898..22400
                     /gene="covS"
                     /locus_tag="VC394_000111"
                     /gene_synonym="csrS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CovS"
                     /protein_id="HEP5993614.1"
                     /translation="MENQKQKQKKYKNSLPKRLSNIFFVLFFCIFSAFTLIAYSSTNY
                     FLLKKEKQSVFQAVNIVRVRLSEVDSNFTLENLAEVLYKNDKTHLRIDDRKGSRVIRS
                     ERDITNTLDANQDIYVYNIDKQMIFTTDNEESSPGLHGPIGRVYHDHIEDQYRGFSMT
                     QKVYSNRTGKFVGYVQVFHDLGNYYVIRARLLFWLLVVELFGTSLAYLIILITTRRFL
                     KPLHNLHGVMRNISENPNNLNLRSDISSGDEIEELSVIFDNMLDKLETHTKLQSRFIS
                     DVSHELRTPVAIIKGHIGLLQRWGKDDSDILEESLTATAHEADRMAIMINDMLDMIRV
                     QGSFEGHQNDMTVLEDSIETVVGNFRVLREDFIFTWQSENPKTIARIYKNHFEQALMI
                     LIDNAVKYSRKEKKIAINLSVTGKQEAIVRVQDKGEGISKEDIEHIFERFYRTDKSRN
                     RTSTQAGLGIGLSILKQIVDGYHLQMKVESELNEGSVFILHIPLAQSKES"
     gene            22615..23109
                     /gene="nrdR"
                     /locus_tag="VC394_000112"
     CDS             22615..23109
                     /gene="nrdR"
                     /locus_tag="VC394_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEP5993615.1"
                     /translation="MRCPKCNYHKSSVVDSRQAEDGNTIRRRRECEQCHTRFTTFERV
                     EELPLLVIKKDGTREQFSRDKILNGVVQSAQKRPVSSTDIENVISRIEQEVRTTYENE
                     VSSTAIGNLVMDELAELDEITYVRFASVYKSFKDVDEIEELLQQITNRVRGKKKRLNN
                     DETN"
     gene            23093..24268
                     /locus_tag="VC394_000113"
     CDS             23093..24268
                     /locus_tag="VC394_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HEP5993616.1"
                     /translation="MMKPIDTFTYLKRNKVTLDSVTLIQLYFPIIGSDAVSIYQYFIH
                     FFDDGLQRHKFSDILNHLQFGMKRFEDALAILTAMELVSVYQLSDTYLITLHQPLSRD
                     LFFQHPAYSRLLEQKIGEVAVSELQVTVPSQARNISKRFSDIFGVQGDLTNVPQKPQK
                     NFDLSSFQQLMVRDGLQFEDNQKDIISLYSIAEQYDMTWFDTYQIAKATAVNGKIRPE
                     RLLAKKNQSMTKPSKENFSQAEQIILREAKQDSALVFLEKIKKARRATITKDERILLQ
                     TLAKMNFLDDVINVMVLYTFNKTKSANLQKSYVLKMANDFAYQKVSTAEEAIVVLRAF
                     TDRQSRRQSKVKTSQSNVPKWSNPDYQETTSQEEQAKLDQFKQAALKRLENLGKGGD"
     gene            24269..25171
                     /gene="dnaI"
                     /locus_tag="VC394_000114"
     CDS             24269..25171
                     /gene="dnaI"
                     /locus_tag="VC394_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEP5993617.1"
                     /translation="MEKIGETMAKLGQNTRVNSDQLIQTILADPEVASFISQHHLSQE
                     QINLSLSKFNQFLVERQKYQLKDPSYIAKGYQPILAMNEGYADVSYLETKELVEAQKQ
                     AAISERIQLVSLPKSYRHIHLPDIDVNNASRMEAFSAILDFVEQYPSAEQKGLYLYGD
                     MGIGKSYLLAAMAHELSEKKGVSTTLLHFPSFAIDVKNAISNGSVKEEIDAVKNVPVL
                     ILDDIGAEQATSWVRDEVLQVILQYRMLEELPTFFTSNYSFADLERKWATIKGSDETW
                     QAKRVMERVRYLAREFHLEGANRR"
     gene            25234..26544
                     /gene="der"
                     /locus_tag="VC394_000115"
     CDS             25234..26544
                     /gene="der"
                     /locus_tag="VC394_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEP5993618.1"
                     /translation="MVLPTVAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRQFSLIDTGGIDDVDAPFMEQIKHQAQIAMEEADVIVFVVSGKEGVTDADEY
                     VSKILYRTNTPVILAVNKVDNPEMRNDIYDFYSLGLGDPYPVSSVHGIGTGDVLDEIV
                     ENLPVEEAEENDDIIRFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DADGQEFTMIDTAGMRKSGKIYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTIDKDNHTVAKWEADIRDQFQFLTYAPIIFVSALTKQ
                     RLNKLPDLIKRISESQNKRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVSVKP
                     PTFVVFVNEEELMHFSYLRFLENQIRAAFTFEGTPIHLIARKRK"
     gene            26751..29849
                     /locus_tag="VC394_000116"
     CDS             26751..29849
                     /locus_tag="VC394_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEP5993619.1"
                     /translation="MMARLIPGRVRNEGIKLYEQGLVSFQDDNKGILQIEVETYQVQY
                     GADDEDITCQCDTFHMKHYCKHIAAVEYFLKNDQKGKLFLKQLTNQTKIKETTKKMTS
                     FGSLFLDSLAMNEDDSVKYRLSALGSRSPFSSDYWWSLKINRLPDDRSYVIRDIKGFL
                     QLIKKEGFYQIGKNYFEQLSWLQFDPSSQDLIEFLWRLASNTDKGDNENIFPNHARHL
                     RLPSGFFEEGIHYLTSLYDFTFEGPSQTYHHLFVRSLEAEAGLYEFKVEVHRKSIELQ
                     IAEKNVQYLFDNDYLLYQDTFYHLTLKQRKMVQAIRSLPIEADLAKHIHFDLDDHAKL
                     AASLSDFKQIGLVEAPKSFAIRDFEVTFQFDLLNRDEISCQLMFDYGNYQVSDKASLE
                     ALPFASHLKKEEKINRSLLAFGFSPQFYSKKRLTSAKELYTFFEETVPCFERLGNVAL
                     STAIQALQVKEMPKIAIRRNQGLLDISFDFSTIIENDIDQAVTALFQNNPYFVSQTGQ
                     LVVFDDETQKVSKSLQELRARQLKNGHLQLDGIRALQVSKLFEGMTSVHFSKELEELA
                     YHLQHPETFSIKPLPVKAQMRDYQRNGVQWLSMLNHYGFGGILADDMGLGKTLQTLAF
                     LASHLKSDSKVLILSPSSLIYNWFDECQKFTPQLDVVVSYGLKQIRDQIIEEGHQITI
                     TSYSSFRQDFETYQAFHYDYLILDEAQVIKNAQTKISHCLRAFNTANCFALSGTPIEN
                     KMLEIWSIFQIVLPGLLPTKKEFLKLTAEQVSRYIKPFVMRRKKEDVLPELPDLIETN
                     YSNEMTDEQKAIYLAQLRQMKDQIRNSSDVDISRQKIEILSGITRLRQICDTPSLFMD
                     YQGKSGKLDSLRILLTQIKENGHRALIFSQFRGMLDLAKQEMTALGLTSYQMTGSTPA
                     NERQEMTRAFNNGSKDAFLISLKAGGVGINLTGADTVILIDLWWNPAVEMQAISRAYR
                     IGQKENVEVYRLITRGTIEEKILELQESKRNLVTTVLDGNENRASMSIEEIKEILGLN
                     TH"
     gene            30092..30694
                     /locus_tag="VC394_000117"
     CDS             30092..30694
                     /locus_tag="VC394_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5993620.1"
                     /translation="MVKNQFPLVADGIAISDPAKQMALYENEDLITNIRGYYQDKEYD
                     DIARNEEFTAKATSRQAPSSKRFCSNDEKHHYVKEARQKAKQDLKEKRQAYLAKEMAY
                     VPKQVSKKQQPADSSPSQKQATTEMSRFTKKLHQDNYILAELPKEYKEPKNLPQQGTT
                     KKNNYDFLKSSQIYNNKEMRQQREKTIAQELNLSRFEDLP"
     gene            30734..32062
                     /locus_tag="VC394_000118"
     CDS             30734..32062
                     /locus_tag="VC394_000118"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEP5993621.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFSEDNITPDMELIVGNAFRENNKEVAYALRHQIPFKRYHDFLGDFMKSF
                     ISFAVAGAHGKTSTTGLLSHVLKNITDTSYLIGDGTGRGSANAQYFVFESDEYERHFM
                     PYHPEYSIITNIDFDHPDYFTGIADVRNAFNDYAKQVKKALFVYGEDDELKKIEAPAP
                     IYYYGFEEGNDFIAYDITRTTNGSDFKVKHQGEVIGQFHVPAYGKHNILNATAVIANL
                     FVAGIDTALVADHLKTFSGVKRRFTEKIINDTIIIDDFAHHPTEIVATIDAARQKYPS
                     KEIVAIFQPHTFTRTIALLEDFACALNEADSVYLAQIYGSAREVDKGEVKVEDLAAKI
                     IKPSQVVTVENVSPLLDHDNAVYVFMGAGDIQLYEHSFEELLANLTKNNQ"
     gene            32108..32590
                     /locus_tag="VC394_000119"
     CDS             32108..32590
                     /locus_tag="VC394_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP5993622.1"
                     /translation="MLIRQVQGSDLEVIATIESDNFSPQEATTRAVLEERIRLIPDTF
                     LVALIDQEIVGYIEGPVVTTPILEDSLFHGVTKNPKTGGYIAITSLSIAKHFQQQGVG
                     TALLAALKDLVVAQQRTGLILTCHDYLISYYEMNGFINQGISESQHGGTLWYQMIWKL
                     "
     gene            32702..34276
                     /gene="mltG"
                     /locus_tag="VC394_000120"
     CDS             32702..34276
                     /gene="mltG"
                     /locus_tag="VC394_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEP5993623.1"
                     /translation="MALTDFKDKDQQDQQRSFKEQILAELEKANQIRKEKEEELFQKE
                     LEAKEAARRTAQLYAEYKRQDAFQKESIAHNNKTAKHFQAIKGAVMTSEALKPTLLSE
                     KENSSLKTTNKRVVQANELQETASKESQVPLTIEKGHSVRRKLSKRQQTERAAKKIST
                     VLISSIIITLLAVTLAGAGYVYSALNPVDKNSDAFVQVEIPSGSGNKLIGQILQKKGL
                     IKNSTVFSFYTKFKNFTNFQSGYYNLQKSMSLEEIASALQEGGTAEPTKPSLGKILIP
                     EGYTIKQIAKAVEHNSKGKTKKAKTPFNEKDFLDLVTDEAFIQDMVKRYPKLLATIPT
                     KEKAIYRLEGYLFPATYNYYKETTMRELVEDMLAAMDATLVPYYDKIAASGKTVNEVL
                     TLASLVEKEGSTDDDRRQIASVFYNRLNSGMALQSNIAILYAMGKLGEKTTLAEDATI
                     DTTINSPYNIYTNTGLMPGPVASSGVSAIEATLNPASTDYLYFVANVHTGEVYYAKTF
                     EEHSANVEKYVNSQIQ"
     gene            34349..34831
                     /gene="greA"
                     /locus_tag="VC394_000121"
     CDS             34349..34831
                     /gene="greA"
                     /locus_tag="VC394_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEP5993624.1"
                     /translation="MAEKTYPMTLTEKEQLEKELEELKLVRRPEIVERIKIARSYGDL
                     SENSEYDAAKDEQAFVEGQISTLETKIRYAEIIDSDAVAKDEVAIGKTVIVQEVGTTD
                     KDTYHIVGAAGADIFSGKISNESPIAQALIGKKTGDKVRIESPAATYDVEIISVEKTN
                     "
     gene            complement(35451..36374)
                     /gene="yidC"
                     /locus_tag="VC394_000122"
     CDS             complement(35451..36374)
                     /gene="yidC"
                     /locus_tag="VC394_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEP5993625.1"
                     /translation="MKLTLNRILFSGLALSILFTLTGCVGRDAHGNPKGMIWEFLGKP
                     MSYFIDYFANNAGLGYGLAIIIVTIIVRTLILPLGLYQSWKASYQSEKMAFLKPVFEP
                     INKRIKQASSQEEKMAAQTELMAAQRAHGINPLGGIGCLPLLIQMPFFSAMYFAAQYT
                     KGVSTSTFMGIDLGSRSLVLTAIIAALYFFQSWLSMMAVSEEQREQMKTMMYTMPIMM
                     IFMSFSLPAGVGLYWLVGGFFSIIQQLITTYLLKPRLHKQIKEEYAKNPPKAYQSTSS
                     RKDVTPSQNMEQANLPKKIKSNRNAGKQRKR"
     gene            complement(36456..36734)
                     /locus_tag="VC394_000123"
     CDS             complement(36456..36734)
                     /locus_tag="VC394_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEP5993626.1"
                     /translation="MQKVRLIVSGRVQGVGFRYATHTLALDIGDIYGRVWNNNDGTVE
                     ILAQSKDSDKIATFIQEVRKGPSKWAKVTYVDVTMANFEDFQDFQIGK"
     gene            36995..37732
                     /locus_tag="VC394_000124"
     CDS             36995..37732
                     /locus_tag="VC394_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEP5993627.1"
                     /translation="MVITSKTNQLIKQTKKLLQKKHRKQSYLIEGWHLFEEAQKSGQV
                     FRHIFVLEEMVERLAGEQELVIVSPQVLKELTDSPSPQGIVAEVEIPKLAFPSDYKGK
                     YLVLEDVQDPGNLGTIIRTADAARFDGVFLSEKSADIYNQKTLRSMQGSHFHLPIWRT
                     DVYQLCRELQEYKTPILATTLSKKSVDYKSLTHHERLALVLGNEGQGISAEMAALADQ
                     LVHITMPGQAESLNVAVAAGILIFSLI"
     gene            37771..38271
                     /locus_tag="VC394_000125"
     CDS             37771..38271
                     /locus_tag="VC394_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEP5993628.1"
                     /translation="MHYTEDKEYMEHVGHLIAHPRFQKLSHIVQHQHSTRLEHSINVS
                     YSSYKLAKRFGWDAKSTARGGLLHDFFYYDWRVTKFNKGHAWVHPRIAVRNAKKLTEL
                     NKKEEDIILKHMWGATIAFPRYKESYIVTMVDKYWAVKEAVTPLRQKWSNRRFLRRKT
                     LQSHNR"
     gene            38286..38975
                     /locus_tag="VC394_000126"
     CDS             38286..38975
                     /locus_tag="VC394_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEP5993629.1"
                     /translation="MNDHVIYTQSDVGLNQFFAKIYSLVGMGVGLSAFVSYLMLYPFR
                     ENLISILVNQPMIYYGAAIIELILVFVASGAARKNTPAALPIFLIYSALNGFTLSFII
                     VAYAQTTVFQAFLSSAAVFFAMSIIGVKTKRDMSGLRKAMFAALIGVVVASLINLFIG
                     SGMMSYVISVISVLIFSGLIASDNQMIKRVYQATNGQVGDGWAVAMALSLYLDFINLF
                     ISLLRIFGRND"
     gene            39153..39395
                     /locus_tag="VC394_000127"
     CDS             39153..39395
                     /locus_tag="VC394_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEP5993630.1"
                     /translation="MSTAIWILLLIVALGVGVFGGIFIARKQIEKEIGEHPRLTPEAI
                     REMMSQMGQKPSEAKIQQTYRNIIKQSKAAVSKGKK"
     gene            39573..40367
                     /gene="racE"
                     /locus_tag="VC394_000128"
     CDS             39573..40367
                     /gene="racE"
                     /locus_tag="VC394_000128"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEP5993631.1"
                     /translation="MDTRPIGFLDSGVGGLTVVCELIRQLPHEKIVYIGDSARAPYGP
                     RPKKQIKEYTWELVNFLLTQNVKMIVFACNTATAVAWEEVKAALDIPVLGVVLPGASA
                     AIKSTTKGQVGVIGTPMTVASDIYRKKIQLLAPSVQVRSLACPKFVPIVESNEMCSSI
                     AKKIVYDSLSPLVGKIDTLVLGCTHYPLLRPIIQNVMGPSVKLIDSGAECVRDISVLL
                     NYFDINGNYHQKAVKHRFFTTANPEIFQEIASIWLKQKINVEHVTL"
     gene            40364..41350
                     /locus_tag="VC394_000129"
     CDS             40364..41350
                     /locus_tag="VC394_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEP5993632.1"
                     /translation="MSEKIYEYKDENNWFIGKMTGHNLISGWGVKHTTIKKIDDLLDG
                     IAATLDWENPKGYDVSVVRYQSPLSLITFIIDMINQETQRKIKVTPHAGTILLMENAK
                     LLAVYLPEGGVSTATFFATSEQGFGDIILIATRNEGKTKEFRNLFGQLGYRVENLNDY
                     PELPEVAETGTTFEENARLKAETISRLTGKMVLADDSGLKVDALGGLPGVWSARFSGP
                     DATDAKNNAKLLHELAMVFDQKKRSAQFHTTLVVAAPNKDSLVVEAEWPGYIATQPKG
                     ENGFGYDPVFIVGETGRHAAELEADQKNQLSHRGQAVRKLMEVFPAWQAKQS"
     gene            41329..41850
                     /locus_tag="VC394_000130"
     CDS             41329..41850
                     /locus_tag="VC394_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEP5993633.1"
                     /translation="MASKTIIVMSDSHGDRDIVQAIKDKYLGQVDAIFHNGDSELNSS
                     DPIWAGIYVVGGNCDYDTGYPDRLVTQLGTVTIAQTHGHLYHINFTWDKLDYFAQEVA
                     ADICLYGHLHRPAAWQVGQTLFMNPGSVTQPRGEINEKLYARVELTDTQIKVDYFTRD
                     HKLYPSLSKEFKR"
     gene            41847..42308
                     /locus_tag="VC394_000131"
     CDS             41847..42308
                     /locus_tag="VC394_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEP5993634.1"
                     /translation="MIAKEFETFLMSHLDNYLIPEQDLAIFIDTHNADHVMLLLVSNG
                     FSRVPVITREKKYVGTISISDIMMYQSKRQLTDWEMSQTDIGEMVNTKIETISITSSL
                     TEIMHKLIDFPFLPVVDRANRFVGIITRKSILKAVNSLLHDFTDDYTIIKK"
     gene            42305..43051
                     /gene="xerD"
                     /locus_tag="VC394_000132"
     CDS             42305..43051
                     /gene="xerD"
                     /locus_tag="VC394_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEP5993635.1"
                     /translation="MKSYIEPFIASKALSQNSQKAYRYDLQQFCQLIGERVNQDKLLL
                     YQNSIANLSLSAKKRKLSTANQFLYYLYQIKYLNSYFRLTDTMKVMRTEKQQAAIINT
                     DIFYQKTPFVWGQLISLLILELGLTPSEVAGIEVANLDLNFQMLTLKTKKGVRVLPLS
                     QILIPFLEQQLVGKEAYLFEHRGIPFSRQWFFNHLKTFVRSIGYEGLTAQKLREQFIL
                     KEKLAGKSIIELSDILGLKSPVTLEKYYKS"
     gene            43051..43755
                     /locus_tag="VC394_000133"
     CDS             43051..43755
                     /locus_tag="VC394_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEP5993636.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSQYKVDIYEVPIVEVIEQYLNYIET
                     LQVMKLEVAGDYMLMASQLMLIKSRRLLPKVVEHIEEEDLEQDLLEKIEEYSRFKAVS
                     QALAKQHDQRAKWYSKPKQELIFEDAILQEDKTVMDLFLAFSNIMAAKRAVLKTNHTV
                     IERDDYKIEDMMASIKQRLEKENVISLSAIFEECQTLNEVISIFLASLELIKLHVVFV
                     EQLSNFGAIILRKEKK"
     gene            43752..44303
                     /locus_tag="VC394_000134"
     CDS             43752..44303
                     /locus_tag="VC394_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEP5993637.1"
                     /translation="MTYLSQIEALLFVAGEEGLSLRHLASMLSLTPTALQQQLEKLSQ
                     KYEKDQHSSLCLIETANTYRLVTKEGFAELLRAYAKTPMNQSLSRASLEVLSIVAYKQ
                     PITRIEIDDIRGVNSSGALSKLLAFDLIREAGKKDVVGRPHLYATTDYFLDYMGINHL
                     DELIEVSAVEPADEEIALFRTQD"
     gene            44425..45147
                     /locus_tag="VC394_000135"
     CDS             44425..45147
                     /locus_tag="VC394_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEP5993638.1"
                     /translation="MRINKYIAHAGIASRRKAEELIKQGLVTLNGQVITDLATTVKSG
                     DVVEIEGSPIYNEEKVYYLLNKPRGAISSVSDDKGRKTVLDLLPQVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDTMIHPRNEIDKVYLARVKGIATKENLRPLTRGIVIDGKKTKP
                     ARYNIVRVEADKNRSIVELTIHEGRNHQVKKMFESVGLLVDKLSRTRFGTVDLKGLRP
                     GEARRLNKKEISQLHNLANTKK"
     gene            45144..45407
                     /gene="yidD"
                     /locus_tag="VC394_000136"
     CDS             45144..45407
                     /gene="yidD"
                     /locus_tag="VC394_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEP5993639.1"
                     /translation="MMKKLLIVSVKAYQKYISPLSPPSCRYKPTCSAYMLTAIEKHGT
                     KGILMGIARILRCHPFVAGGVDPVPEDFSLMRNKNTSKNAEKA"
     gene            45585..46133
                     /locus_tag="VC394_000137"
     CDS             45585..46133
                     /locus_tag="VC394_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEP5993640.1"
                     /translation="MTTKELINKNDKVKKARNHIVLFQPQIPQNTGNIARTCAATNAP
                     LHIIKPMGFPIDDRKMKRAGLDYWDKLELHFYDHLEQFINQCHGQLHLISKFAVNNYS
                     QATYADGDSHYFLFGREDTGLPEDFMREHAEKALRIPMNDEHVRSLNVSNTVCMVIYE
                     ALRQQGFQGLELKHTYEHDKLK"
     regulatory      46242..46366
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            46445..47008
                     /locus_tag="VC394_000138"
     CDS             46445..47008
                     /locus_tag="VC394_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEP5993641.1"
                     /translation="MSKTHKMIMIGILSAISFLLMLVSFAIIPGAAFLKIEFSIIPVL
                     FGLMIMDLKSAYLILLLRSLLKLFLNNRGVNDFIGLPMNIIAIALFVTAFALVWNRQK
                     TLSQYVFASLLGTGLLTFGMVVLNYTFAIPLYAIFANIDIRAYIGVTKYMMTMVIPFN
                     LVEGLIFAITFYFVYIASKPILERYLH"
     gene            47010..47663
                     /locus_tag="VC394_000139"
     CDS             47010..47663
                     /locus_tag="VC394_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEP5993642.1"
                     /translation="MMTNKQTHFLIASFALLIFVIIGYTVKFFPERLALLDNTIQAEI
                     RGNLPIVLTQFFRGVTVFGNVMTQVLLVIVSVLVLFFMKWKIEALFILSNGAIAAFLI
                     TTLKLFYQRPRPAIEHLVYAGGYSFPSGHAMGSMLIFGSLLIICYQRLHSKLLQFVTS
                     MIFIILILLIGLSRIYLGVHYPSDILAGFVLGFGILHFIYPFYKQKRFEWRFLLKQD"
     gene            47956..48876
                     /locus_tag="VC394_000140"
     CDS             47956..48876
                     /locus_tag="VC394_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407327.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEP5993643.1"
                     /translation="MKKRYQTLNEHYRQLFGAKMFKVPIDAGFDCPNRDGTVAHGGCT
                     FCTVSGSGDAIVAPDAPIKEQFYKEIDFMHRKWPDVNRYLVYFQNFTNTHDTVDVIRD
                     RYEQAINEPGVVGINIGTRPDCLPDDTIAYLAELSERMHVTVELGLQTTYEETSRLIN
                     RAHSYDLYKETVRRLRHYPNINIVSHLINGLPKETHDMMLENVRRCVTDNDIQGIKLH
                     LLHLMTNTRMQRDYHEGRLKLLSQKDYVSIICDQLEIIPKHIVIHRITGDAPRDMLIG
                     PMWSLNKWEVLNAIDKEMERRGSFQGCKVD"
     gene            48915..49469
                     /locus_tag="VC394_000141"
     CDS             48915..49469
                     /locus_tag="VC394_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEP5993644.1"
                     /translation="MLKRPIHLSHDFLAEVVDKSSVVVDATMGNGNDTAFLAQLAKKV
                     YAFDVQEQAIRKTSERLAQLGLSNAELILAGHEAVDQYVTEPVRAAIFNLGYLPSADK
                     SIITLPNTTLQALSKLLTLLMVGGRIAIMVYYGHDGGSLEKDALLDFVKQLDQRKVSA
                     MLYQPLNQVNTPPFLIMLEKLADV"
     gene            49602..50936
                     /locus_tag="VC394_000142"
     CDS             49602..50936
                     /locus_tag="VC394_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEP5993645.1"
                     /translation="MEDPVSQPLVIQFLLLVVLTLLNAFFSASEMALVSLNRSRVEQK
                     AADGDKKYARLLRVLEEPNHFLSTIQVGITFISLLSGASLSASLGKVISGWLGNSATA
                     RTAGTIISLVFLTYVSIVLGELYPKRIAMNLKDKLAIVSAPIIIGLGRLVSPFVWLLS
                     ASTNLLSRLTPMTFDDADEQMTRDEIEYMLSKSEATLDAEEIEMLQGVFSLDEMMARE
                     VMVPRTDAFMIDINDDPLENIQEILKQSFSRIPVYDVDKDKIIGLIHTKRLLESGFRQ
                     GFDQINMRKMLQEPLFVPETIFVDDLLRQLRNTQNQMAILLDEYGGVAGLVTLEDLLE
                     EIVGEIDDETDKAEQFVHEIGDNTYIVVGTMTLNEFNDYFDTELESDDVDTIAGFYLT
                     GIGTIPSQEQKEAYEIDNKDKHLVLINDKVKDGRITKLKLILSNIEQIIEED"
     gene            51014..51805
                     /gene="pflA"
                     /locus_tag="VC394_000143"
     CDS             51014..51805
                     /gene="pflA"
                     /locus_tag="VC394_000143"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEP5993646.1"
                     /translation="MTEKDYGQVTGMVHSTESFGSVDGPGIRFIIFLQGCKLRCQYCH
                     NPDTWEMETNNSKIRTVNDVLKEALQYKHFWGKKGGITVSGGEAMLQIDFITALFIEA
                     KKLGIHTTLDTCGFTYRPTPEYHQVLDNLLAVTDLILLDLKEIDEKQHKIVTRQPNKN
                     ILQFARYLSDKQIPVWIRHVLVPGLTDIDDHLTRLGEFVKTLKNVDKFEVLPYHTMGE
                     FKWRELGIPYQLEGVKPPTKERVQNAKNLMQTESYTEYMNRIHQS"
     gene            51936..52871
                     /locus_tag="VC394_000144"
     CDS             51936..52871
                     /locus_tag="VC394_000144"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEP5993647.1"
                     /translation="MSKILVFGHQNPDTDAIASSYAFDYLSQKAFGLDTEVVALGTPN
                     EETAFALDYFGVEAPRVVESAKAQGSEQVILTDHNEFQQSIADIREVEVYGVVDHHRV
                     ANFETANPLYMRVEPVGSASSIVYRMFKENGIEVPKAIAGMLLSGLISDTLLLKSPTT
                     HVSDHLVAEELAELAEVNLEDYGMALLKAGTNLASKSEVELIGIDAKTFELNGNAVRV
                     AQVNTVDIAEVLERQEAIEAAIKDAMAAEGYSDFVLMITDIVNSNSEILAIGANMDKV
                     EAAFNFTLDNNHAFLAGAVSRKKQVVPQLTESFGA"
     gene            52947..53600
                     /locus_tag="VC394_000145"
     CDS             52947..53600
                     /locus_tag="VC394_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEP5993648.1"
                     /translation="MITVFHSDKLTRQPFFQDLINYLDQHDHVILREIKKAFPNVTGI
                     DKAIESYVQAGYIRRENKRYGINLPLVSSDQQLALDTMLFVDTCSAMYENILAVVFET
                     QLTNQTNHVMIKEKTNITRDDLTLANYFYRLKRGEKPSAEQLDLYDLLGDVNQEYALK
                     YMTTFLLKFMRKDLVMQKRPDIFVEALVTLGYLKQVEPTTYQLLMTLDKESLTFIAP"
     gene            complement(53645..54682)
                     /locus_tag="VC394_000146"
     CDS             complement(53645..54682)
                     /locus_tag="VC394_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEP5993649.1"
                     /translation="MLFMVNKKRRPLLKSHRHLLGLYTSLILLLVSLMGLALSLGESH
                     LSFLDLIHVFLGKSSHAISFIVINIRLPRILAACLGGGSLALSGLLLQRLTRNPLADS
                     GVLGITIGAGISLAIVVSFSFFEQAHISHYLPLFAMLGAVVTTFSVYWLSLTKQGQID
                     PTRLILTGVAVTTMLSSLMVALVGHINRYKVDLVINWLSGQLIGDDWPTLSVIAPLLL
                     CFWLLTYSQAHFLNIMGLADNTAIGLGLPLNRKRRLILVLAAGLGALSVLLVGNISFI
                     GLIAGHFSTYLVGSNHKITIPISILIGMILLLVADTVGRVYLVGTNIQTGILVSLIGA
                     PYFLYLMAKTK"
     gene            complement(54643..55695)
                     /locus_tag="VC394_000147"
     CDS             complement(54643..55695)
                     /locus_tag="VC394_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEP5993650.1"
                     /translation="MKTKHPFLCLERFCLKKTQIITKSHIFWLVFVLLSFSLCVAIYC
                     HLRFGAVALSHQDLNSILFGKQNGHKANVLLAIRLPRLFGATLTGSALAVSGTIMQAI
                     TRNPIAEPGLLGINAGAGLALVLAYAFVPHLHYSLIILLSLLGSSLAATLVFGLSYQS
                     GKGYHQLRLVLAGAMVSILLSALGQGITNYYHLANAVIGWQAGGLVGVNWQMIGYIAP
                     LIILSLCLAQLLSYHLTVLSLSESQAKALGQKTNLISAVFMILVLILSSAAVAIAGSI
                     SFIGLVIPHLMKHFTPHHYRYLLPLCAVSGASFMVWVDIACRNLNPPYETPLGALVSL
                     IGFPCFLWLIRRGGRY"
     gene            complement(55685..56617)
                     /locus_tag="VC394_000148"
     CDS             complement(55685..56617)
                     /locus_tag="VC394_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-hydroxamate ABC transporter
                     substrate-binding protein"
                     /protein_id="HEP5993651.1"
                     /translation="MKKLTLLLTLCLTTITLIACGNQATNHSNTASKSLSPMPQIAGV
                     TYYGDIPKQPKRVVSLASTYTGYLKKLDMNLVGVTSYDKKNPILAKTVKKAKQVAATD
                     LEAITTLKPDLIVVGSTEENIKQLAEIAPVISIEYRKRDYLQVLSDFGRIFNKEGKAK
                     KWLKDWKTKTAAYEKEVKAVTGDKATFTIMGLYEKDVYLFGKDWGRGGEVIHQAFHYD
                     APEKVKTEVFKQGYLSLSQEVLPDYIGDYVVVAAEDDKTGSALYESKLWQSIPAVKKH
                     HVIKVNANVFYFTDPLSLEYQLETLREAILSSEN"
     gene            complement(56643..57425)
                     /locus_tag="VC394_000149"
     CDS             complement(56643..57425)
                     /locus_tag="VC394_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5993652.1"
                     /translation="MTTISAEDLTIAYEQRTIIDKLSFYIPEGKITTIIGANGCGKSS
                     LLKALTRLLPPKQGVVYLNGQNIATLETKEVAKKLALLPQVQEATNGITVYELVSYGR
                     FPHQSYFGNLSPADKKAIHWAMQATNVMAYADQPVDALSGGQRQRVWLAMALAQGTDT
                     IFLDEPTTYLDLNHQLEILELVKSLNKDAGKTIVMVLHDLNLSARYSDHLIAMKHGKI
                     HYTGTIADVMTSPIIQDIFQIKPVLVDDPIHNCPIVLTYQLQ"
     gene            complement(57671..59116)
                     /locus_tag="VC394_000150"
     CDS             complement(57671..59116)
                     /locus_tag="VC394_000150"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEP5993653.1"
                     /translation="MITIEQLLDILKKDHNFREVLDADGYHYHYQGFSFERLSYDSRQ
                     VDGKTLFFAKGATFKADYLKEAITNGLQLYISEVDYELGIPVVLVTDIKKAMSLIAMV
                     FYGNPQEKLKLLAFTGTKGKTTAAYFAYHMLKESYKPAMFSTMNTTLDGKTFFKSQLT
                     TPESLDLFAMMAECVTNGMTHLIMEVSSQAYLVDRVYGLTFDVGVFLNISPDHIGPIE
                     HPTFEDYFYHKRLLMENSRAVVINSGMDHFSFLADQVADQEHVFYGPLSDNQITTSQA
                     FSFEAKGQLAGHYDIQLIGHFNQENAMAAGLACLRLGASLADIQKGIAKTRVPGRMEV
                     LTMTNHAKVFVDYAHNGDSLEKLLSVVEEHQTGKLMLILGAPGNKGESRRADFGRVIH
                     QHPNLTVILTADDPNFEDPEDISKEIASHIARPVEIISDREQAIQKAMSLCQGAKDAV
                     IIAGKGADAYQIVKGQQVAYAGDLAIARHYL"
     gene            59204..60838
                     /locus_tag="VC394_000151"
     CDS             59204..60838
                     /locus_tag="VC394_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEP5993654.1"
                     /translation="MSTEKKQLTQEELMVQGAAWSTAGNFISRLLGVLYIIPWYIWMG
                     QYAIQANALFNMGYNVYAYFLLISTTGLNVAIAKQVAKYNSMGQTEHSYQLIRSTLKL
                     MLGLGLIFSAIMYLGSPLFASLSGGDDTLVPIMHSLSLAVFIFPVMSVIRGIFQGHNN
                     IKPYAISQIAEQLIRVIWMLLTTFLIMKLGSGDYASAVTQSTFAAFIGMVASMGVLGY
                     YLWKQGLLAAIFSKPDHTVSIDIKGLLLETLKESIPFIVTGSAIQAFQLIDQWTFVNT
                     MTLFTDYSRSQLLVLFGYFNANPAKITMVLIAVAASIGGVGIALLTENYVKKDMKAAA
                     RLIINNIEMLVMFLLPALTGAIILARPLYSVFYGASEDRAIHLFVAVLFQTLLMALYT
                     LFSPMLQALFENRKAIYYFAYGILIKLVLQIPLIYLLHAYGPLLATTIALVVPIYLMY
                     RRLYQVTHFNRKLLQKRLLLTLIETLLMGLVVFVANWLLGYVFKPTGRLTSLLYLLII
                     GGLGMTVYTALTLLTHQLDKLIGSKASRLRQKLGWH"
     gene            61006..61635
                     /gene="upp"
                     /locus_tag="VC394_000152"
     CDS             61006..61635
                     /gene="upp"
                     /locus_tag="VC394_000152"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEP5993655.1"
                     /translation="MGKCQVISHPLIQHKLSILRRQTTSTKDFRELVNEIAMLMGYEV
                     SRDLPLEDVDIQTPVSKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRNEETLEPVEYLVKLPEDINQRQIFLVDPMLATGGSAILAVDSLKKRGAANIKFVCL
                     VAAPEGVKKLQEAHPDIDIFTAALDDHLNEHGYIVPGLGDAGDRLFGTK"
     gene            61859..62449
                     /locus_tag="VC394_000153"
     CDS             61859..62449
                     /locus_tag="VC394_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002274529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEP5993656.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDNTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGTKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRHRLEKILAQNAGK
                     TIKQIHKDAERDYWMSAEETLTYGFIDEIMENNELK"
     gene            62944..63219
                     /locus_tag="VC394_000154"
     CDS             62944..63219
                     /locus_tag="VC394_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEP5993657.1"
                     /translation="MFQKQERIGLVVYLYYNRDARKLSKFGDLYYHSKRSRYLIVYIN
                     KNDLDTKLEEMRRLKCVKDIRPSAFDDIDRQFVGNLHRDETNNHQKG"
     gene            63468..64103
                     /locus_tag="VC394_000155"
     CDS             63468..64103
                     /locus_tag="VC394_000155"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEP5993658.1"
                     /translation="MITGKLITVEGPDGAGKTTVLEQLIPLLKQKVAQEILTTREPGG
                     VAISEYIRELILDINHTTMDPKTELLLYIAARRQHLVEKVLPALEAGQLVFIDRFIDS
                     SVAYQGAGRGLIKADIQWLNEFATDGLEPDLTLYFDVPSEIGLARINANQQREVNRLD
                     LETIEIHQRVRKGYLALAKEHPKRIVTIDATKPLKEVVSVALEHVLALLLA"
     gene            64121..64996
                     /locus_tag="VC394_000156"
     CDS             64121..64996
                     /locus_tag="VC394_000156"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEP5993659.1"
                     /translation="MDLAQKAPNVYQAFQTILKKDRLNHAYLFSGDFANEEMALFLAK
                     VIFCEQKKDQTPCGHCRSCQLIEQGDFADVTVLEPTGQVIKTDVVKEMMANFSQTGYE
                     NKRQVFIIKDCDKMHVNAANSLLKYIEEPQGEAYIFLLTNDDNKVLPTIKSRTQVFQF
                     PKNEAYLYQLAQEKGLLNHQAKLVAKLATNTSHLERLLQTSKLLELITQAERFVSIWL
                     KDQLQAYLALNRLVQLATEKEEQDLVLTLLTLLLARERAQTPLTQLEAVYQARLMWQS
                     NVNFQNTLEYMVMSE"
     gene            65499..65822
                     /gene="yabA"
                     /locus_tag="VC394_000157"
     CDS             65499..65822
                     /gene="yabA"
                     /locus_tag="VC394_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEP5993660.1"
                     /translation="MNKKELFDAFDGFSQNLMVTLAEIEAMKKQVQSLVEENTILRLE
                     NTKLRERLSHLEHETVAKNPSKQRKDHLEGIYDEGFHICNFFYGQRRENDEECMFCRE
                     LLDRK"
     gene            65827..66690
                     /gene="rsmI"
                     /locus_tag="VC394_000158"
     CDS             65827..66690
                     /gene="rsmI"
                     /locus_tag="VC394_000158"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEP5993661.1"
                     /translation="MQVQKSFKDKKTSGTLYLVPTPIGNLQDMTFRAVATLKEVDFIC
                     AEDTRNTGLLLKHFDIATKQISFHEHNAYEKIPDLIDLLISGRSLAQVSDAGMPSISD
                     PGHDLVKAAIDSDIAVVALPGASAGITALIASGLAPQPHVFYGFLPRKAGQQKAFFED
                     KHHYPETQMFYESPYRIKDTLTNMLACYGDRQVVLVRELTKLFEEYQRGSISEILSYL
                     EETPLKGECLLIVAGAQVDSEVELTADVDLVSLVQKEIQAGAKPNQAIKTIAKAYQVN
                     RQELYQQFHDL"
     gene            66717..67109
                     /locus_tag="VC394_000159"
     CDS             66717..67109
                     /locus_tag="VC394_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5993662.1"
                     /translation="MTEDQATILILGVYGIILAVFLLSALLCLIANWRLFKRAGYKGW
                     YSLIPFYAGFVKHKITFGEDNKWFYFIGWVIAIYYYYVHFCYVRAFGGSKGLAVFGLF
                     FPGIASLIVAFGKTYTTQECHPVPHLLN"
     gene            complement(67156..67785)
                     /locus_tag="VC394_000160"
     CDS             complement(67156..67785)
                     /locus_tag="VC394_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEP5993663.1"
                     /translation="MIKEFCAENLTLLPTLDAGQVSRVELCDNLAVGGTTPSYGVIKE
                     ACQLLHDKKISVATMIRPRGGDFVYNDLELKAMEEDILKAVEAGSDALVLGLLTSENQ
                     LDTDAIEQLLPATQGLPLVFHMAFDRIPTDHQHQALDQLIDYGFVRVLTHGSPEATPI
                     TDNVEQLKSLVTYANKRIEIMIGGGVTAENCQSLSQLTGTAIVHGTKII"
     gene            68084..68440
                     /locus_tag="VC394_000161"
     CDS             68084..68440
                     /locus_tag="VC394_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEP5993664.1"
                     /translation="MITIYQYPKCSTCQRAMAELKQLVSDFEAIDIKANPPKAQDLKH
                     WMETSGYTIKNFFNTSGNSYRELGLKDKIDQLSLDKAAELLATDGMLIKRPILIKDGN
                     VLQVGYRKPYQELDLG"
     gene            complement(68514..69341)
                     /gene="xth"
                     /locus_tag="VC394_000162"
     CDS             complement(68514..69341)
                     /gene="xth"
                     /locus_tag="VC394_000162"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEP5993665.1"
                     /translation="MKLISWNIDSLNAALTGESPRALLSRAVLDTLVAQDADIIAIQE
                     TKLSAKGPTKKHIETLLSYFPNYLHVWRSSVEPARKGYAGTMFLYKNTLNPVITFPEI
                     GAPTTMDAEGRIITLEFEDFFVTQVYTPNAGDGLRRLDDRQIWDHKYADYLTELDAQK
                     PVLATGDYNVAHKEIDLANPSSNRRSPGFTDEERQGFTNLLARGFTDTFRHLHGDIPH
                     VYTWWAQRSKTSKINNTGWRIDYWLASNRIVDKVKRSEMISSGERQDHTPILLDIDL"
     gene            69575..70756
                     /gene="lctO"
                     /locus_tag="VC394_000163"
     CDS             69575..70756
                     /gene="lctO"
                     /locus_tag="VC394_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HEP5993666.1"
                     /translation="MAQKTVITEETTDFVMDFKTSSAEGNVDFINVFDLEKMAQQVIP
                     KGAFGYIASGAGDTFTLHENIRSFNHKLIVPHSLKGVENPSTEITFDGDHLTSPLILA
                     PVAAHKLANEQGEVASAKGLKEFGSIYTTSSYSTTDLPEISAALGGTPHWFQFYCSKD
                     DGINRNIMDRVKAQGCKAIVLTADATVGGNREVDRRNGFVFPVGMPIVQEYLPDGAGK
                     TMDYVYKSAKQALTSKDIEYIATYSGLPVYVKGPQCAEDTLRALDAGASGIWVTNHGG
                     RQLDGGPAAFDSLQEVAEAVDHKVPIVFDSGIRRGQHIFKALASGADLVALGRPAIYG
                     LAMGGSIGTRQVFEKLNDELKMVMQLAGTQTIQDVKAFNLRHNPYDSSIPFDQNALRL
                     D"
     gene            71022..75959
                     /gene="scpC"
                     /locus_tag="VC394_000164"
     CDS             71022..75959
                     /gene="scpC"
                     /locus_tag="VC394_000164"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CXC chemokine-degrading serine protease SpyCEP"
                     /protein_id="HEP5993667.1"
                     /translation="MEKKQRFSLRKYKSGTFSVLIGSVFLMMTTTVAADELTTTSEPT
                     ITNHAQQQAQHLTNTELSSAESQSPDTSQITPKINREKEQPQGLVSEPTTTELADTDA
                     APMANTGPDATQKSASLPPVNTDVHDWVKTKGAWDKGYKGQGKVVAVIDTGIDPAHQS
                     MRISDVSTAKVKSKEDMLARQKAAGINYGSWINDKVVFAHNYVENSDNIKENQFEDFD
                     EDWENFEFDAEPKAIKKHKIYRPQSTQAPKETVIKTEETDGSHDIDWTQTDDDTKYES
                     HGMHVTGIVAGNSKEAAATGERFLGIAPETQVMFMRVFANDVMGSAESLFIKAIEDAV
                     ALGADVINLSLGTANGAQLSGSKPLMEAIEKAKKAGVSVVVAAGNERVYGSDHDDPLA
                     TNPDYGLVGSPSTGRTPTSVAAINSKWVIQRLMTVKELENRADLNHGKAIYSESVDFK
                     DIKDSLGYDKSHQFAYVKESTDAGYNAQDVKGKIALIERDPNKTYDEMIALAKKHGAL
                     GVLIFNNKPGQSNRSMRLTANGMGIPSAFISHEFGKAMSQLNGNGTGSLEFDSVVSKA
                     PSQKGNEMNHFSNWGLTSDGYLKPDITAPGGDIYSTYNDNHYGSQTGTSMASPQIAGA
                     SLLVKQYLEKTQPNLPKEKIADIVKNLLMSNAQIHVNPETKTTTSPRQQGAGLLNIDG
                     AVTSGLYVTGKDNYGSISLGNITDTMTFDVTVHNLSNKAKTLRYDTELLTDHVDPQKG
                     RFTLTSRSLKTYQGGEVTVPANGKVTVRVTMDVSQFTKELTKQMPNGYYLEGFVRFRD
                     SQDDQLNRVNIPFVGFKGQFENLAVAEESIYRLKSQGKTGFYFDESGPKDDIYVGKHF
                     TGLVTLGSETNVSTKTISDNGLHTLGTFKNADGKFILEKNAQGNPVLAISPNGDNNQD
                     FAAFKGVFLRKYQGLKASVYHASDKEHKNPLWVSPESFKGDKNFNSDIRFAKSTTLLG
                     TAFSGKSLTGAELPDGYYHYVVSYYPDVVGAKRQEMTFDMILDRQKPVLSQATFDPET
                     NRFKPEPLKDRGLAGVRKDSVFYLERKDNKPYTVTINDSYKYVSVEDNKTFVERQADG
                     SFILPLDKAKLGDFYYMVEDFAGNVAIAKLGDHLPQTLGKTPIKLKLTDGNYQTKETL
                     KDNLEMTQSDTGLVTNQAQLAVVHRNQPQSQLTKMNQDFFISPNEDGNKDFVAFKGLK
                     NNVYNDLTVNVYAKDDHQKQTPIWSSQAGASASAIESTAWYGITARGSKVMPGDYQYV
                     VTYRDEHGKEHQKQYTISVNDKKPMITQGRFDTINGVDHFTPDKTKALGSSGIVREEV
                     FYLAKKNGRKFDVTEGKDGITVSDNKVYIPKNPDGSYTISKRDGVTLSDYYYLVEDRA
                     GNVSFATLRDLKAVGKDKAVVNFGLDLPVPEDKQIVNFTYLVRDADGKPIENLEYYNN
                     SGNSLILPYGKYTVELLTYDTNAAKLESDKIVSFTLSADNNFQQVTFKMTMLATSQIT
                     AHFDHLLPEGSRVSLKTAQGQLIPLEQSLYVPKAYGKTVQEGTYEVVVSLPKGYRIEG
                     NTKVNTLPNEVHELSLRLVKVGDASDSTGDHKVMSKNNSQALTASATPTKTTTSATAK
                     ALPSAGEKMGLKLRIVGLVLLGLTCVFSRKKSTKD"
     gene            76732..77439
                     /locus_tag="VC394_000165"
     CDS             76732..77439
                     /locus_tag="VC394_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEP5993668.1"
                     /translation="MSKKKRKHYQVYDGLRCAMTLCFISGYVNAFTYMTQGKRFAGVQ
                     TGNLLSFAIRLSEQQLKEALQFLLPMIVFMLGQSFTYFMHRWATKKGLHWYLLSSVIL
                     TGIAFGMALFTPFLPSNVTVAALAFFASIQVDTFKTLRGASYANVMMTGNIKNAAYLL
                     TKGLYEKNHELTHIGRNTLIVILAFAVGVVCSTLLCIAYGEYALMPILMPLLYVNYLL
                     AQEFYHIQTKIKPIKRT"
     gene            77679..79679
                     /gene="metG"
                     /locus_tag="VC394_000166"
     CDS             77679..79679
                     /gene="metG"
                     /locus_tag="VC394_000166"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEP5993669.1"
                     /translation="MMKKPFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGHEV
                     FYLTGLDEHGQKIQTKAKEAGITPQTYVDNMAKDVKALWQLLDISYDTFIRTTDDYHE
                     EVVAAVFEKLLAQDDIYLGEYSGWYSVSDEEFFTESQLEEVFRDEDGKVIGGIAPSGH
                     QVEWVSEESYFLRLSKYADRLVAFFKERPDFIQPDGRMNEMVKNFIEPGLEDLAVSRT
                     TFTWGVPVPSDPKHVVYVWIDALLNYATALGYGQANHANFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDLPMPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERFGLDPLRY
                     YLMRSLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVAMINKYFDGTVPAYVDNGT
                     AFDADFSQLIDAQLADYHKHMEAVDYPRALEAIWTIIARTNKYIDETAPWVLAKEDGD
                     KAQLASVMAHLAASLRVVAHVIQPFMMETSAAIMAQLGLAPVSDLSTLALADFPANTK
                     VVAKGTPIFPRLDMEAEIDYIKAQMGDSSAISQEKEWVPEEVALKSEKDVITFETFDA
                     VEIRVAEVKEVSKVEGSEKLLRFRVDAGDGQDRQILSGIAKCYPNEQELVGKKLQIVA
                     NLKPRKMMKQYISQGMILSAEHGDQLTVLTVDSSVPNGSIIG"
     gene            80170..81183
                     /gene="nrdF"
                     /locus_tag="VC394_000167"
     CDS             80170..81183
                     /gene="nrdF"
                     /locus_tag="VC394_000167"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEP5993670.1"
                     /translation="MTQHYYERSQSPIEYALSETQKQLRSINWNYLNDDKDLEVWNRV
                     TQNFWLPEKVPVSNDLNSWRSLGEDWQQLITRTYTGLTLLDTVQATVGDVAQIQHSQT
                     DHEQVIYTNFAFMVGIHARSYGTIFSTLCSSEQIEEAHEWVVSTQSLQDRSRVLIPYY
                     TGDDPLKSKVAAAMMPGFLLYGGFYLPFYLSARGKMPNTSDIIRLILRDKVIHNYYSG
                     YKYQQKVARLSPEKQAEMKAFVFDLLYELIDLEKAYLRELYAGFDLAEDAIRFSLYNA
                     GKFLQNLGYDSPFTDEETRISPEVFAQLSARADENHDFFSGNGSSYVMGITEETTDDD
                     WEF"
     gene            81187..81675
                     /gene="nrdI"
                     /locus_tag="VC394_000168"
     CDS             81187..81675
                     /gene="nrdI"
                     /locus_tag="VC394_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEP5993671.1"
                     /translation="MAELIIVYFSSKSNNTHRFVQKLGLPAQRIPVDNRPLEVSTHYL
                     LIVPTYAAGGSDAKGAVPKQVIRFLNNPNNRKHCKGVISSGNTNFGDTFALAGPIISQ
                     KLQVPLLHQFELLGTATDVKKVQAIFARLKHHTHDKQKQINNLITERTHPCHKPMRHT
                     SH"
     gene            81642..83822
                     /gene="nrdE"
                     /locus_tag="VC394_000169"
     CDS             81642..83822
                     /gene="nrdE"
                     /locus_tag="VC394_000169"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEP5993672.1"
                     /translation="MSQTNASYLSLNALTRFKKPDGSYHFDSDKEAVRRYLEEHVSPN
                     QMAFNSLEDKLAYLINEGYYEQAIFDAYPNDLIKEAFHYAYQQGYRFLNLMGAMKFYQ
                     SYALKTLDGKQYLETFEDRAVMNALFLADGDQTFVFDVIDAILHRRFQPATPTFLNAG
                     KKRRGEYISCYLLRVEDNMESISRAISTSLQLSKRGGGVALCLTNLREIGAPIKGIEN
                     QATGIVPVMKLLEDSFSYANQLGQRQGAGAVYLHAHHPEVLTFLDTKRENADEKIRIK
                     SLALGLVIPDITFQLAKENKDMALFSPYDIKRAYGKDMSDISITEEYDKLLANPAIKK
                     TYISARKFFQLIAELHFESGYPYLLFDDTVNKRNPHAKKGRIVMSNLCSEIAQVSTPS
                     TFKEDLSFETIGEDICCNLGSINIAQAMADAPHFEQLITTSIRALDRVSRVSDLNCAP
                     SVETGNAANHAVGLGAMNLHGFLATNHIYYDTKEAVDFTDLFFHAMAYYAFKASCQLA
                     KEKGAFAGFSLSTYADGTYFAKYLQEDAKPQTAKVATLLQDYGFTLPTVADWQALVAD
                     IKQFGLANAHLLAVAPTGSISYLSSCTPSLQPVVAPVEVRKEGSLGRIYVPAYQIDQA
                     NYAYYERGAYEVGPKAIIDVVAAAQKHVDQAISLTLFMTDQATTRDLNRSYIQAFKQN
                     CASIYYVRVRQDVLAGSEQYDEDSLVTAPGASDETTTECQSCMI"
     gene            complement(84027..84779)
                     /locus_tag="VC394_000170"
     CDS             complement(84027..84779)
                     /locus_tag="VC394_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosyltransferase"
                     /protein_id="HEP5993673.1"
                     /translation="MLKKRYQLAMILLLSCFSLVWQTEGLVELFVCEHYERAVCEGTP
                     AYFTFSDQKGAETLIKKRWGKGLVYPRAEQEAMAAYTCQQAAPINTSLDKTKGELSQL
                     TPELRDQVAQLDAATHRLVIPWNIVVYRYVYETFLRDIGVSHADLTSYYRNHQFDPHI
                     LCKIKLGTRYTKHSFMSTTALKNGAMTHRPVEVRICVKKGAKAAFVEPYSAVPSEVEL
                     LFPRGCQLEVVGAYVSQDQKKLHIEAYFKGSL"
     gene            85236..85688
                     /locus_tag="VC394_000171"
     CDS             85236..85688
                     /locus_tag="VC394_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5993674.1"
                     /translation="MKTKSKRFLNLATLCLALLGTTLLMTRPVKAEVSEIGHDHVTIS
                     SNGQTDEGAAYGRGHDDGSKFGYEAGLQSSWNESEPPSSDKIPEPSVNPYESSNEQDR
                     EDYKEGFRDGYPGGYVAGWRKTHPIEATLQYLWYTVSSWFESLFNNSK"
     gene            complement(85995..87247)
                     /locus_tag="VC394_000172"
                     /pseudo
     CDS             complement(85995..87247)
                     /locus_tag="VC394_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293804.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            87703..88176
                     /locus_tag="VC394_000173"
     CDS             87703..88176
                     /locus_tag="VC394_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5993675.1"
                     /translation="MKTKSKRFLNLATLCLALLGTTLLMGQPIKAEAVVSDLNDAVSS
                     MGDTAEETDVNIDGQEDEEDEEDDAYMQGRRLGEKEGYEAGQKDNAPRLFDSSTLEPP
                     TNPYEDAGDTLNSNWFKSGYSDAYGQAYEQGWNDTHPIQSTLIWLWGIVRNLFRF"
     gene            88429..88680
                     /locus_tag="VC394_000174"
     CDS             88429..88680
                     /locus_tag="VC394_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5993676.1"
                     /translation="MPEKKVLPVLSMVVSFVCLLDHIFFFATYSYLYLPLVASSLALI
                     SLVQNKGRQKVWSLAGLILAALAVAVVAYAYYDPTVIPY"
     gene            complement(89053..89553)
                     /locus_tag="VC394_000175"
     CDS             complement(89053..89553)
                     /locus_tag="VC394_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5993677.1"
                     /translation="MKKTLTLLLALFTIGVTSSVRAEESSWVPLPRQQKVLDDNRNEI
                     PVHITGNKVTVTLPPGWSAWIDKNTKQRNSNSVTTTVTLDISTSQPPKEFTIYFQGDD
                     GFLAGMMTFIDSETLKNEQYKAFDEEYAENLFENSRLREEQKTWRDKVRDVFQDAWWN
                     FKGLFQ"
BASE COUNT        28453 a        16197 c        18539 g        26606 t
ORIGIN      
        1 gtaatcattt tttgatttac agttattatc atgtgttatc tggcgactgt cctgtttaca
       61 agtaagttca caagtatggg ataaaatagt ataaactata gtaaatactg aatagtcata
      121 gaaaaatatc ggcataggtc agaaaagctg atatgtttaa tactttttgc ggatatctac
      181 tgtatttaaa tgtatttgat atccttgtca aggatatacg tctaaacaag ttcaaaacga
      241 aatcatttgt gattatctta tcaggagttg actcttgata agatttttgt atatcagtac
      301 tgtggtacgt ttagatagct tggcagtgaa tttatagagt gtcaaacaac gagtgttttg
      361 tgttacacta accattagaa gagtctattg ttgagcatat caaggtgtta tcttttcgag
      421 ttgaggtatc atatctagta tatttaacta tggttttaaa tgctatttca aaaaaaatat
      481 gataaaatag aaagtactaa ttattttgga gatgacatga gtatcgatga ttataggccg
      541 acctatatgg tagaggctat ttatgattta agagcaaatg atttgttgcg tcatggtatt
      601 acggctgttt tagtggattt agataatacc ttaattgcat ggaataatcc tgatggcaca
      661 ccagaggttc gcgcttggct tgatgaaatg actatcgcgg atattagcgt ggttgttgtt
      721 tcaaataata aacattctcg tgttgagcgt gccgtttcac gctttggtgt tgactttatt
      781 agccgggctt tgaagccatt tgcttatggt attgaaaaag ccattgctcg ttatggattt
      841 gatcgcaacg aggttatcat ggttggagat cagctaatga ctgatatcag agctagtcat
      901 agagctggaa taaaatcagt tttagtcaaa ccacttgttg cttctgatgc ttggaatact
      961 aaaattaacc gctggcgtga acgccgtgtg atggcaaaat tagaagagaa atatggtaaa
     1021 ttatcatatc aaaaaggaat ttaatggaag aattattttg tataggctgt ggtatccaga
     1081 ttcaaactga agacaaagaa aaagctggtt ttacgccagc tgctgctttg aaaaagggta
     1141 tggaaacagg agaactctat tgtcagcgct gctttagatt gaggcattat aatgaaatta
     1201 cagatgtcca tattaccgat gatgaatttt taagacttct tcacgaagtg ggtgatagtg
     1261 atgctcttgt ggttaatgtg attgatattt ttgactttaa tggttctatt attccaggtc
     1321 tatctcgctt tatttctggt aatgatgtct tactggttgg gaataaaaaa gatattttgc
     1381 caaagtctgt taaggatggt aaagttactc aatggttgac ggaacgtgca cacgaagaag
     1441 gtcttcgccc tttagacgtt atgttaacga gcgctcaaaa taaatatgcg attaaagact
     1501 tgattgagcg tatcaatgag ttacgtaatg gtcgagatgt ttatgttgtg ggggtcacta
     1561 atgttggtaa atcgacctta attaatgcca ttattcaaga aattacaggg gataaagatg
     1621 tgattacgac gtcccgcttc cctggaacga cccttgataa aattgaaatc ccactggatg
     1681 acggtagctt catatttgat acgccaggta ttattcatcg tcatcaaatg gctcattatt
     1741 tatcccctaa agagctaaaa atagtgagtc ctaagaagga aatcaagcct aagacgtatc
     1801 aattaaaccc agaacaaact ctctttttag gtggtttgtc tcgctttgac tttatcaatg
     1861 gggagcgtca agggtttaca gctttttttg acaatcaact agaattacat cgtacaaaat
     1921 tagctggagc agatgctttt tatgataagc atgttggaac tttgttaact ccgccaggta
     1981 agaaagagtt gacagcattt cctaaattag ttcgtcatga atttacaatt gatcagaaaa
     2041 tggatattgt tttttctggt ctaggatgga ttcgtgttaa tggtcaaaaa gactcaaaag
     2101 caatagttgc tgcatgggct cctgaaggcg tcgctgtcat tgttcgtaaa gcgattattt
     2161 aaaaataaaa aaagaagttg gaaaaaaatg cttacaagta aacaaagagc tttcttaaaa
     2221 tctgaggctc atagcttaaa accgattgta caaattggca aaaatggatt aaatgaccac
     2281 attaagacta gtattcgtca agctttggat gctagagaat tgataaaggt cactttgttg
     2341 caaaatactg atgaagatat tcatgaagta gcagagattt tagaagaaga aattgggtgt
     2401 gatacggttt tgaaaattgg tcgtatcttg attctctata aggtttctgc taagaaagaa
     2461 aaccgcaagt tgtcccctaa agttaaagca atctagtcct tttatttgta tagggactgg
     2521 ttttatgaga cgaggactat tgtcgcgaga attttggaga aagtatggca ttagaattgt
     2581 tgacgccttt tacgaaggtg gaattagagg aagaaaaaaa agaaagcaac cgcaaacaga
     2641 ttgggatttt aggagggaat tttaatccta ttcacaatgc acaccttgtg gttgcagatc
     2701 aagttcgcca gcaattagga ttagatcagg tgctattgat gcctgaatgt aaaccaccgc
     2761 atgtggatgc caaagagact attgatgaga agcatcgtct tcgcatgttg gaattggcta
     2821 ttgaagatgt tgaagggcta gctattgaaa cctgtgaact tgaacgtcag gggatcagct
     2881 atacctatga tacgatgctt tatttgactg agcagcatcc tgatgttgat ttttatttta
     2941 tcatcggagc tgatatggtt gattatttgc caaagtggca ccgtattgat gaactggtca
     3001 aactcgttca atttgttgga gttcaacgtc caaagtataa agctggaacc tcttacccgg
     3061 tcatttgggt agatcttcct ttgatggata tttcttctag tatgattagg gattttatta
     3121 aaaaaggtcg tcagcccaat tatctcttac ctaagcgggt tttagactac attactcaag
     3181 aaggtttgta tcaatgacat atgaagatta tcttccttat agcaggaccg aactgttagc
     3241 aaaaattgca gagcagatga gccctaaacg gtttaaacat gtacttggcg tggaaaaggc
     3301 agccttgtcc ttggcagagt gttatggttg taacccagac aaagcagggt tagcagctct
     3361 tttacatgat tatgccaagg agtgccctga ccaggtcttt ttagacttaa ttgacaagta
     3421 ccaattatct ccagaattag ctaaatggaa taataatgtc tggcatggta tggttggtat
     3481 ttataagatt caagaagacc tagggctaaa agacaaagat atcttgcgag ctattgaaat
     3541 tcacaccgtt ggtgctgctg agatgacctt gctggataag gtcttatacg ttgctgatta
     3601 tattgaagaa ggtcgcattt ttcctttggt tgatgacgct agaaaaatcg cgaagctcga
     3661 tttgaatcag gctgttgcct atgaaacagt gaacactgtc gcttatttag cctcaaaagc
     3721 gcagcctatt ttcccacaaa ctctggatac ctataatgcg ttttgcagct atttaaagga
     3781 atactaatgg ataatgatat gaggaataaa atgaaaaaag aagaactctt aaaaattgtt
     3841 gtggaagcaa ctgatgaaaa gcgtgctaaa gatattttag ccttggattt agaggggtta
     3901 actagtttga ctgattattt tgtgattgcg agtgcaacaa atagtcgtca attagaggct
     3961 attgcggata atatccggga aaaggtaaaa gaggctggtg gagatgctag ccatgttgaa
     4021 ggtaatagcc aagcaggctg ggtcttgctt gatttgaccg atgtggtggt acatcttttt
     4081 ttagaagatg agcgttacca ctataatctt gaaaaattgt ggtatgaagc gcctgctgtg
     4141 gctctagatg cctatttagc ttaatgttac aaagagacct tgtcaatacg acagggtttt
     4201 tgaggatgat aatgaaacat aattatgaaa aatttgcgtc agtttatgat gctgtgatgg
     4261 atgattctct atatgacttg tggacagatt tttcacttcg acatttacca aagtctaagg
     4321 gtcgaaatcg tttgttagag ttggcctgtg gaaccggtat tcagtcagtt cgttttgctc
     4381 aagcaggttt tgatgtgact ggtttagact taagtcaaga catgctggct attgctaaaa
     4441 agcgtgcgca atctgctaaa aagaaaattg attttattca aggtaacatg ctggatttgt
     4501 cacaggtggg gcagtttgat tttgtaacct gctattcgga ttccatttgc tatatgcaag
     4561 acgaagttga tgttggcgat gtttttaaag aagtttatga tgttttagca aacgatggca
     4621 tctttatttt tgatgtgcat tcaacctatc aaacggatga gtgtttccct ggttattcct
     4681 atcatgagaa tgcagatgat tttgcaatgg tttgggatac ctatgctgac gaggctcctc
     4741 actctgttgt tcacgaactc actttcttta tccaagaaga cgatggacgt ttttctcgct
     4801 ttgatgaagt gcatgaggaa cgaacgtatg agttattgac ttatgatatt ttgttggaac
     4861 aagcagggtt caagtcattt aaggtctatg ctgattttga agataaggag ccaactaaga
     4921 ccagcaagcg ttggtttttt gtagcatata aatagaagaa aaaagtagcc tttttagcta
     4981 taggatttaa tgaattatct tgaatgttac ttatatgaaa agagttcatt aggcattaaa
     5041 tgctattatc aaagcttaga agtattggag acattacgat tgacactatg tctgaaggtt
     5101 gctaccaact ttaaagaaag cattttctat tatacgttgt gcttaagcat ttgatttaac
     5161 tcttattagc cctataaaag actatttagt acaatttgag gagattctat gactgttacc
     5221 ggaattatag ctgagtttaa tccgtttcac aatggacaca aatatttgct agaaacagca
     5281 gagggactaa aaattattgc tatgtcagga aattttatgc aaagaggaga gcctgctctt
     5341 attgataaat ggattcgatc agagatggct cttaaaaatg gtgcggatat tgttgtggaa
     5401 ttgccctttt tcgtttcagt tcaatcagca gactattttg ctcaaggagc tattgatatt
     5461 ctttgtcaat taggaattca gcaattagca tttgggacag aggatgtgat tgattatcaa
     5521 aaactgatta aggtctatga gaaaaaatca aagcaaatga ctgcctattt atcgacgctt
     5581 gaagatacac ttagctatcc acaaaaaaca caaaaaatgt gggaaatctt tgctggagtt
     5641 aaattttcag ggcaaacgcc aaatcatatt ttggggttaa gttacgcaaa ggctagcgct
     5701 ggaaagcata tacaactatg tcctatcaag cgtcaagggg ctgcctacca ttcgaaagac
     5761 aaaaatcatc tgctagcttc tgcaagtgcc attagacagc acctgaatga ttgggatttc
     5821 ataagtcatt cagtaccaaa tgcaggttta cttataaaca atccccatat gtcatgggac
     5881 cattattttt cattcttaaa atatcaaatt ctgaaccatt ctgatttaac tagtattttt
     5941 caggtcaatg atgaattggc aagtcgtatt aaaaaagcga taaaagttag tcaaaatatt
     6001 gaccacctag ttgacactgt tgctaccaaa cgctatacaa aagctcgtgt aagacgtatt
     6061 ttaacctaca ttttggttaa tgctaaggaa cctactttac ctaaaggaat tcatattcta
     6121 gggtttacct caaaaggaca agcacattta aaaaaattaa aaaaaagcag gcctctgatt
     6181 actagaattg gagcagaaac ttgggatgag atgacacaaa aagcagacag catctatcaa
     6241 cttgggcatc aggatatccc agaacaaagt tttggaagaa tacctattat aataaaaaat
     6301 gagaggctca attaaactct aattgagttt ttttgggggc ttgactctaa cattacgttg
     6361 atggtttata cttcagttaa tccaatgagg aaattttata acaataatta aaaaagtcag
     6421 tcaaaagata tgctttcgaa gaacaggcta acgtttttac aagattatat cagtaatcat
     6481 tttgacaatt gtagtaatgg aatgttgcca aaatttagaa tgttgtgtgt tatgaatgac
     6541 aagattttaa aggaatacta attaaacggg aggtcgtgaa ccggaagctg ttgtcactca
     6601 agcgccaaaa tctgtctgta acataatatt gatgtatggg aacgcaatta tttggttttt
     6661 attgtgatgc ttaaaacgat tttatatttc tattttacca aagcttttca aagtgctata
     6721 ataagggagt tagagtcaat attagtaacg ttaatgttga caatattcaa taaaatacta
     6781 ttaaaaggag acaaaaatgg gacgtaaatg ggcaaatatt gttgctaaaa aaactgctaa
     6841 agatggagca acatcaaagg tctatgccaa atttggtgtt gaaatttatg tggctgccaa
     6901 acaaggcgag ccagatccag agttaaatac agctcttaaa tttgtcattg atcgtgcaaa
     6961 gcaagcacaa gtgccaaagc atgttattga taaagcgatt gataaagcta aaggaaacac
     7021 agatgaaact ttcgtagagg gacgctatga aggttttggt ccaaatggtt caatgattat
     7081 tgtggatact ttgacatcaa atgttaaccg tacagcggca aatgtacgta cggcttacgg
     7141 taagaacggt ggcaatatgg gagcttcagg atctgtatcc tacttatttg ataaaaaagg
     7201 ggtcatcgtt tttgctggtg atgatgctga cagtgtcttt gaacaattac ttgaagcgga
     7261 tgtagacgta gatgatgttg aagcagaaga gggaacaata acagtttata ccgccccaac
     7321 agatcttcat aaaggtatcc aagcacttcg cgataatggt gtagaagaat tccaagttac
     7381 tgaacttgaa atgattcctc aatcagaagt agtattggaa ggtgatgacc ttgaaacttt
     7441 tgaaaagctt attgatgcac tcgaatcaga tgatgatgtt caaaaagtgt atcataatgt
     7501 agcagatttt taaattgaca tagaataata aaaagagttg aaaactattt cagctctttt
     7561 taagtgatgc ttttataagt aagtaccaag taacatagtc gttgttagaa gataatgaac
     7621 aatagtaaat cagtatcaac ttgataggaa tcaattatta gtgaactcta attaaaaatg
     7681 gtaaactaga cacaagaaca aaaaggagag ataggatgat taaaaaacaa tatttaggaa
     7741 gggcaacaat tgccctagca tcaacccttg tactagcagc ttgtgggtca tcaaaaacag
     7801 ctgagtcagg aaatcagggg agttctaaag aagtcttatt tgcaacggtt ggaacaacag
     7861 ctcccttttc ttatgaaaaa ggtggtcaac taactggcta cgatatcgag gtggctaaag
     7921 cagtctttaa aggatcggat gattacaggg tgtcctttaa aaaaacagaa tggtcttcta
     7981 tctttacagg tttggactca ggaaaatacc aaatgggtgg taacaatatt tcctttacca
     8041 aagagcgttc agctaagtac cttttttctt atccaatcgg atcaacacct tctgttttag
     8101 ttgttccaaa agacagtgat atcaagagct ttgatgatat ccaagggcat acgacacaag
     8161 ttgttcaagg gactacaagt gttgcacagt tagaagactt taataagaaa cacagtgaca
     8221 acccggttac tctaaaattc accaatgaga acattaccca aatgttaacc aatttgagtg
     8281 aaggcaaggc agatttcaag atttttgacg ctccaacggt taacgccatt attaaaaatc
     8341 aagggttaga taatctcaaa acaattgaac tcacctcaac agagcaacca tttatttatt
     8401 ttatctttag tcaagatcaa gagaaattgc aaagctttgt taacaagcgt attaaagagt
     8461 tgactgctga cggaactctt agcaagcttg ccaaagagca ccttggcggt gattacgtgc
     8521 catcagataa agagttgaaa cttcctacag ctaactaaac catctattaa ccagttattc
     8581 atctagaaga ctggtttttc tgatcggaaa caatattata gtttatgagg ttatgttata
     8641 aaaattgcaa atagccttaa tcaaaatgag atgttaaagt gctttttgag aagtgaatat
     8701 tgtaagaagt atgctttggg ttttagtcct caaataccaa gttttttgtt gactaaacaa
     8761 ataaggatgg ttagcatagc atatagttta aaactatggt atatgctagc cattatcaat
     8821 ttgaaaacta tatctaatta agatacaata atgatcgtaa acaaaatgaa tcgaggataa
     8881 cgctatgtct tttaaaaaat ggattggaat agtcggaatc gctttagcat caactgtatt
     8941 ggttgcttgt tctgaaaaac aagatgataa aaacactcta actattggag tgatgacaaa
     9001 aacagaatct gatcaagcga gatgggataa agttgaagaa ttattgaaaa aagacaatat
     9061 cactttgaag tacaaagaat ttacagatta ctcgcaacct aataaggcag ttgctaatgg
     9121 tgaagtcgat attaatgcct tccaacacta taatttctta aataactgga ataaggaaaa
     9181 taaggaacat ttggtggcca ttgctgatac ctatatcagc ccaattaacc ttttctctgg
     9241 aaccagtcaa gatgggaagg ctaaatacaa atcagtagct gatctgccaa atggtgctca
     9301 aattgcagta ccaaatgatg cgaccaatga aagccgtgcc ctctatgtgc tgcaatcagc
     9361 tggcctcatt aaattgaatg tgtctggtga tcaattagca accattgcca atatctcaga
     9421 gaacaaaaag aaattagata tcaaggaatt agatgctagc caaacggcac gtgctttagt
     9481 atctgctgat gcagctgttg tcaataatag ttacgctgtt cctgcaaaaa ttgactacaa
     9541 aacctctctt ttcaaagaaa aagcagatga caattcaaaa caatggatta acattattgc
     9601 aggtcaaaaa gattgggaaa aatcagagaa ggctgatgct attaaaaaac tcatcaaggc
     9661 ctaccaaact gatgaggtga agaaagtcgt ggaaaaaact tcaaatggta tagatgtatc
     9721 cgtatggtaa attttagcca ttgttataac aagagtgtgg tcgtgtttaa aacacatgct
     9781 ttcttgcatg cttgaggaag agtttacgac taatttacct ttagaaagct atttaagacc
     9841 ttttcccttt taggatttag aaaccttggg aagactattg catgttatct taatagtcat
     9901 gattatgcag gcgatgaaaa cattgctatc gtagattact gtcgccatat cgtactaata
     9961 gaggagttat taaaatctta tgaatgaagc aataattcaa ttagatcata ttgatattac
    10021 cttccgtcaa aaaaaacggg ttatcgaagc tgttaaagat gtcacggttc atatcaatca
    10081 aggagatatt tatggcattg ttgggtattc aggtgccggg aaatcaactc ttgtgcgtgt
    10141 gattaacctg ctacaggcac caacaaacgg gaaaatcact gttgatgggg atgtgacctt
    10201 tgaccagggg aaaattcaat tgtcagctga tgcccttcgc caaaaacgac gtgatattgg
    10261 tatgatcttc caacatttca atttgatggc ccaaaaaaca gccaaagaaa atgtagcatt
    10321 tgcgctccgt cattcgtcgt taagtaagac tgagaaagaa cataaagtta tcgagctgct
    10381 agaacttgta ggtttatcag agcgagcaga taactaccct gcccagttat cgggtgggca
    10441 aaaacaaagg gtggctattg cgcgtgctct agctaacgat ccaaaaattt tgatttcaga
    10501 tgaggcaaca tcagcactag atccaaaaac cactaaacaa attttggcgc tcttgcaaga
    10561 attaaaccgc aaacttggcc ttaccattgt tatgattact catgaaatgc aaattgttaa
    10621 ggatatttgt aatcgtgttg ctgttatgca aaatggtgtt ttgattgaag aaggttctgt
    10681 ccttgatatc ttttcaaatc caaaagaagc cttgacacag gaatttatca caacagctac
    10741 agggattgat gaggctttag aaaaaattaa ccaacaagac attgttaaac atttgcctgc
    10801 aaatgccctt ctagctcagt tgaaatatgc aggaacttct acggatgagc ctctacttaa
    10861 tagcatttac cgtcaatttg aagtgacagc taatattttg tatggcaata ttgagatttt
    10921 agatcatatt ccagttggtg acatgattgt tgtcttggaa gggcaagctg aaaatattct
    10981 tgcggctgag aaagccttgc atgaagctgg tgttgatgtt agtattttaa agagaggagc
    11041 ctagatgtca cagttaattc aaacttattt gccaaatgtt tatgaactcg gatggtctgg
    11101 agatgctggt tggggcttag cgatttggaa tactttatac atgaccattg tgccttttat
    11161 tgttgggggt gctattggtc tcttattagg cttattatta gttttaacag gaccggacgg
    11221 tgttattgaa aacaaaacga tttgttgggt tattgataaa gttacctcta gttttagagc
    11281 cattcctttt gttattttga ttgctatttt agctagcttt acctatttgc ttttaagaac
    11341 aactttagga gcaacagcgg ccttggtacc tttaaccttt gcgacttttc ctttttatgc
    11401 ccgtcaggtc caagttgttt tttcagaatt agacaagggt gttattgaag cagcgcaagc
    11461 ttcaggtgct actttctggg atattgtcaa agtgtatctc agtgaaggtt taccagatct
    11521 tattcgtgtt tctacagtca ccttaatttc tttagttggg gaaactgcca tggcaggagc
    11581 tatcggtgct ggaggattag gaaatgtagc gatttcttat ggttataatc gttttaacaa
    11641 tgatgtgact tgggtagcga ctattattat tcttttaatt atttttgcta tccaatttat
    11701 tggtgatagc ttgactagaa ggtttagtca taaataaacg aaacaacacc ctaagttttt
    11761 acttagggtg ttgttgttta gatgatgtca ttagcttttc ttcgttttac ttagtataag
    11821 ccctattaaa aaaccaatag cagcaaagat aatccatccc attgagaatt gccctagtgg
    11881 aacagttttt tggaaaaaag ttacaacggc ttctggtaaa tgaaataatc cagtcatttg
    11941 tgatagtgtt aggagtgcat caaatatggc aggaataaag gttagaccaa ttgtagttcg
    12001 atagacaata ggatcattat tgaagcattt ttgtgccaaa acaaggaaaa ttaaatcgac
    12061 tgttaatggg tataaaagga ataaaacagg agctgaccag ttgataataa ctgataaacc
    12121 accaaaataa aagaaagctg atagtaaagt aaagatagtt gcccaagcaa tatgagataa
    12181 agcaggcact aatttatgga agtattcagc acttgacgtg attaagcctg ttgaagtggt
    12241 tagacaggcc aggaaaatca caacagctag aaatgcttgt ccgatgccac ctaggtaaaa
    12301 acgagaagca tgacttaaga tttgaccgcc attaactgga ttaccatgaa gggtaaagct
    12361 gccatcacta aaaggaaata atgattgtga tgttgctcca atacgaccga caaagatata
    12421 gacaagtgct aatagcaaaa tggcaatagc cccagaaata agtgttattt tggtcacttc
    12481 tttgtccgtc ttagcgccaa attgtttggt agcctcaatg actaaaatag caaaaacaag
    12541 agaagctagt gcatccatag tgccataacc ttgaattaat cctgctataa aaggaaaatc
    12601 tttaaaggca ttattaacac cgaccccagc gttaaaagca tcaccgtaat ttccagcagg
    12661 atggacaaag gacgcgataa ccaaaataga aataacaact aacaacgttg gcgttaaaaa
    12721 ttttccgata ttttcagcta gcttactagg tttgatagca aggaagtagg acagaccaaa
    12781 aaatagtata gcataaatag ctttattggt tgtattatct cctaagatag gagcgatacc
    12841 gactgaaaaa gaagtggctc ctgttcttgg aatagcaaaa aatggaccaa tagataaata
    12901 caaaatggaa gaatagaaga ttgcatacca tttagagatg gggcgtgcca aactttcaac
    12961 atcgcctgat ccagatttag caacagcaat gacaccgagt aaaggcaagg agacaccagt
    13021 taaacaaaaa ccaatgatag accatagaat ctgatgacct gagtagatgc ctaaaaaagc
    13081 tgggtaaatg aggtttgctg ctccaaaaaa tagtgcaaat agcatgaatc caatgacgat
    13141 atatacattt ttttgtttca tttataaata gtctttctat tgatactaat ccgaagattc
    13201 taacaatttt aattatatta tgtgtcaaaa taacaacaaa ggttattata tctaagatta
    13261 cttcgatagt caatagaaat tgactgaaaa ttcttagcac aaaaaatggt taagatatgt
    13321 agctcattcg atttgacgaa tagatagaat tatattatta taaaaaacat gtaactttag
    13381 taaggatgta acgtatgaaa aaaatatatg atttatgggt tagggttagt ctaatcaaaa
    13441 aaataggtat cggtgttgta ataggagtca tgttgggcat tttggctcct gatcttacag
    13501 gttttagtat tttaggaaag ttatttgttg gtggtttaaa ggctatcgca cctttgcttg
    13561 tatttgcttt ggtttcccaa gccatctccc atcagaaaaa agggaaacag actaatatga
    13621 cattaatcat tgttctgtat ttatttggta cctttgcgtc agctttagta gcagtactga
    13681 cagcttatct attccctttg acgttagtgt taaatacacc tgtaaataca gaattatcac
    13741 cacctcaagg tgttgctgaa gtttttcagt cacttctatt aaaattagtg gataatccga
    13801 tcaatgcttt agctactgcc aattatattg gtgtgctatc atgggcaatt atttttggac
    13861 tagcattaaa ggctgcaagt caagaaacaa agcatcttat aaaaacagcg gcagaagtta
    13921 cttcacaaat agttgtttgg atcatcaatc tggcacctat tgggattatg agtctggtgt
    13981 tcactacgat ttctgaaaac ggtgttggaa tcttatctga ttatgctttt ttgatactag
    14041 ttttagtagg aacaatggtt tttgtcgcct tagtggttaa tccacttatt gcggttttga
    14101 ttacacgaca aaatccttat ccccttgtct tgagatgttt acgtgagtca ggtcttacag
    14161 catttttcac acgaagttct gcagccaata ttccagtcaa tatgcaatta tgccaaaaaa
    14221 ttggattgag taaagatact tattcagtgt caattccact tggagcaact attaatatgg
    14281 gtggggcagc aattaccatc aatgtcctaa cgcttgctgc tgtacacact tttggtattc
    14341 ctattgattt tctaacagcc ctcttactta gcgttgttgc tgctgtctct gcttgtggtg
    14401 cttctggtgt tgctggggga tcgttgttac ttattcctgt agcatgtagt ttgtttggca
    14461 tctcaaatga tcttgcgatg caggtggttg gtgttggttt tattgttggt gttatccagg
    14521 attcttgtga gacagcctta aattcgtcaa ccgatgttct ttttacagct attgctgaaa
    14581 atgctttttg gaaacgtaaa aaagcctagc taggtttaac atagtaaaaa acatccttgg
    14641 aaaacgccaa ggatgttttt ttattattta agatagccaa gataatcaaa tttttcaaag
    14701 gtgtggtcat tagcgatggc tacaatgata gtattcggtt ctaaaggttc aaaaggtttg
    14761 acattggtat ctaaggtttt aacttctttt tgacgcatgc caatcacatt taactcatat
    14821 ttacgacgaa catctagttc ggaaagagat tgtccttccc agcttttagg aatgacaaat
    14881 tcaatcatgg aaatgccatg ttcgaggtag atgatgcttt cgatatgacg tcttaacaga
    14941 tttgaagcta cacgttttcc agaatcacgt tcaggggtga tgactttagt tgccccgatg
    15001 ccatatagaa cttcttcaaa aattttattt ttagccttgg caataatggt tggaacaccg
    15061 agttttttac agtgcatgac tgcaagaaca gaagattcca agttattccc tgaagcgata
    15121 acgacagtat cacaatgttc aatccctaca gctaatagaa attctttgtc agtaatatct
    15181 ccaacagcgg ctttagtgac caagtcggcc acctctttaa catgactttc tctgatatca
    15241 atagcaataa cgtcttgatc aaaattactt aattctctgg caacagtacg gccaaaaata
    15301 ccaaggccaa gaacgccgac agttttacgt tttaacatgt tttctcctta acccactaaa
    15361 atatcagtag tggcatattg aatggttttt tcttttcgtt gaatcagact gatgaggaca
    15421 gtaattgggc caacgcggcc gacgaacatc aagacaatga caataagacg tccagcagta
    15481 gacaattgtg gtgtcaaatc catagatacc ccaactgtcg cgatggcaga aatagattca
    15541 aataagagtg gtataggagc gatatgaggc tccacgctaa gtaataaaat aaaacctatc
    15601 ataagaactg caaaaaagaa gataagaact gtcatggtct gcttaattgt cttatttgcg
    15661 ataattcggt tacgaaaggt aacttctgat tgccctgaaa gttccgcttt aaagagtaag
    15721 aaagtaatgg cagcagtcgt cactttaata ccaccagctg ttccaccagg agcaccacca
    15781 ataaccattt gaatcatata aagaatgtta gtcggtgcta gggtatcgtt ataagagatt
    15841 gttgcaaagc cagctgttct catagtgact gtttgaaaaa atgaaaccat aagttgctga
    15901 tgtaaactaa aattggcaat ggttttgctg ttatcttttt ctaaaaacca ggttaagaag
    15961 gttccaagaa aaagaataac agctgtggtt tgtagtacta gacgagactg attggataat
    16021 ttacgaaaag ttgccccata acaatgaggt ctttcaaaaa agtatttttt aaaagctact
    16081 cctaaatcaa cccaaaccgc aaaaccaagt ccaccagaga taatcaagaa cgtaatgata
    16141 acatttaatg taggatttaa cataaagtct tttaaactag aagatcctaa attgtcaaaa
    16201 ccagcgttac aaaatgccga cacagctaga aaaatactat tgaagatacc atttttccac
    16261 ccgaagcggg ggataaagtc aatcatgata actatcgcag caaaggcttc tatactaaag
    16321 gttactttat aagcaaaaaa aaggtagtgc tttaaatctt tgctatcacc tcgatttaac
    16381 gctgattgga gcaaggtttg atcgctcaat cgcatttttc gcttgagggc aaaagtactc
    16441 acagctatta acgtcaccaa gccaagacca ccaatctgca ttagtgccat agcaatggtt
    16501 tgtccgatac cgttatacac ttcagcgacg ggaacaacag aaagtccagt cacacaaacc
    16561 atggaaacca cattaaagaa atgatcaaga tagacggtat taggaccatt ttgataatgg
    16621 gtaaagggca tggataacaa gagagtccca atcagaatca caatggcaaa actaaatgtt
    16681 aagcgctgtg tgactgaaag agatttgata aaagagcgtt tcatgtttcc tccagacata
    16741 atatctccta ctataataac aaaaataagt taaaaaagct aagtttcatc ttagcctttg
    16801 gtactatttt atagaggttt tttgttaggc aagccagctt ttcttggata cttgttaggt
    16861 gtttcctttt tcttctcaac aatggtgatg aagcgagaat ctccatttgg caattggtag
    16921 ctatggtttt taatgacttt accaaaaaga agacaaagag cattcttagc ttcttctaac
    16981 tcttggtcag cagcttgtgc ctttaaggcg attaattttc ctccaatttt taaaaagggg
    17041 atggttaatt cagatagaac ctgcatccga gcaaccgctc tggcagtaac gacatcaaat
    17101 tgacctcgaa aggccttgtc ctgtccgaaa tcttctgctc taccatgaaa aaaatggaca
    17161 ttttccaagc ccaattcctg agcaaggagt gtcagaaaag aaatccgctt attaagagaa
    17221 tcaataatgg taacctcaag gttcgggaaa agaattttca tgggtaggct aggaaaacca
    17281 gctccagctc caatgtccaa taatttgatt ggttcattag ctaaaaagcc ctgtaaaata
    17341 ggagcgatgg aatcataaaa atgcttgagg taaacctcat tttcttctgt aatagctgtt
    17401 aaattaattt tagtgttcca ttccactagt aatttaaagt aggtgtcaaa ttgttctttt
    17461 tgtttcgatg ataaggaaaa accatcttcc tctagtgttc ggtaaaagtc ttggggtgtc
    17521 atttgtatcc tctgtttttg atagttgtta ccattttatc ataaaatagc ccataacaaa
    17581 atccaataaa tcatcagaaa taacacctca ttacttgcta ttagcctaga aattgctata
    17641 attgagacac aaagattaaa ggagacccac tatgccaaca gttttgatta tcttagtcgt
    17701 tttaggagtg cttgcgctat ggttaatgat tagttacaat agcctggtta aatctcggat
    17761 gcacacaaaa gaagcttgga gtcagattga tgtgcaatta aagcgtcgta atgacttgat
    17821 tccaaatctt attgaaaccg taaagggata tgctagctac gagcaaaaga catttgaaaa
    17881 aatcactgat ttgcgtgcgc gtgttgcgaa tgcctcaact cctcaagaaa ccatggcggc
    17941 ttctaacgaa ttgagtaaac aagtgaccag tttgtttgcc gttgctgaaa attacccaga
    18001 cttaaaagct aacgaaaact tcttgaaatt acaagaagag ttgaccaata cggaaaataa
    18061 aatctcatat tctcgtcaac tctataattc aacaacgtct aattacaacc ttcaattaga
    18121 atctttccca agcaatatcg ctggtaaatt atttggtttt aaaccaagtg aattcttaca
    18181 aacaccagaa gctgaaaaag aagttccaaa agttgaattt aacttttaaa tatgaaatta
    18241 tcatctagta ggcaatcata agagaaagga gaattatgct ttatcaacag atttcacaaa
    18301 ataagcaacg aacagttgtt ttattagttg ttttcttcgc tctcttagca cttattggag
    18361 cttcagcagg ctatctgctg ttagataatt atgccatggg cttggttctt gctcttgtca
    18421 ttggggtgat ttatgctacc agtatgattt ttcaatccac cagtcttgta atgagtatga
    18481 acaatgctag agaagtcaca gaaaaagagg ctccaggctt cttccatatt gtcgaggata
    18541 tggctatggt ggctcagatt ccgatgccga gagtttttat tattgaagat ccttctttaa
    18601 atgcttttgc gacaggatct agccctcaaa atgctgctgt tgcagcaacg acaggtttac
    18661 ttgaagtcat gaatcgtgaa gaacttgaag gtgttatcgg ccatgaaatt agccacatcc
    18721 gaaattatga tatccgtatt tcgactattg ctgtagcttt ggcgagtgct gttaccgtta
    18781 tttctagtat cggtgggcgt atgctatggt atggtggcgg ttctcgcagg caaagagatg
    18841 acggagatga tgatgtttta agaatcatta cattactttt gtcgttatta tctttgcttt
    18901 tagcgcccct agtagcaagt ttgattcagt tggctatttc tcgtcaaaga gaatatctag
    18961 ctgatgctag ttctgttgaa ctaacaagaa atccccaagg tatgattaac gctcttgaaa
    19021 aattgcagtt atctcagcca atgaagcatc ctgttgatga tgctagtgca gccttgtata
    19081 ttaatgagcc tcgcaaaaaa aggagcttca gttcattatt cagcacccat cctcctattg
    19141 aggagaggat tgaaaggcta aaaaacatgt gagcgtttgg ggtttttgct caaacctcac
    19201 ttttttatgt ctttgggcag ataatctaaa atggcaggaa ttatcaaaaa tatctgaaca
    19261 aaaagaaaaa aatgagtgaa acaatggtta aaaatagaat gatttaaatc ttaaaattaa
    19321 aaaacgtcag aaaataaatg caaaactatt gacaaatctt ttagatagta gtaaaaataa
    19381 atgagagttt tattaggaga agatgatgtt agctatttcc gaaatcagaa aacacccaga
    19441 cggtctctcg tttgatcgtt tatgtgatgt caaaagtatg ttacttgagc gtgaccaaca
    19501 gattattgac ataaaggcag ttaaagctgt cggtaatgtt cgctatgata aggggcttta
    19561 tctgttagat tatcaattga gttacgaagt gatattaccg tctagccgtt ctatggttcc
    19621 ggtctgttta agtgaagtcc aacacattca agaattattt atagaggcga cagatttagc
    19681 tgataagaaa gagttggttg aagacaatct ggtattagtt ttagacaaag acgctattaa
    19741 cctcgaagaa agtattgtgg ataatatctt acttgctatt ccgctacagg tcttgacaga
    19801 ggaagaaaag aaatcaaaag agttgccagc aggtcaaaat tgggcagttt tgacagaaga
    19861 agactaccag tgtctaaaag aagaaaagca aaaagaaaat aatccttttg ctagcttgca
    19921 agggttgttt gatgaataaa actgaatatt aaagatgtct gtaattttta tttgaaaaaa
    19981 cagctattct ttgatatact ctttagagaa tatggttact tatctaaagt agaaggtatt
    20041 ttgaccatag agggcaaaga aggtaatgac tattagatgc ttcatatttt attcgctaga
    20101 agactatttg accattagaa ctgaaatagt ctaggatatg agatgaattt catagttttc
    20161 tttctgggag aaaaagatag attaagagga taagggttgg tataaatgac aaagaaaatt
    20221 ttaattattg aagatgaaaa gaatctggct agattcgttt ctcttgagct gcaacatgag
    20281 ggttacgaag tcattgttga ggtcaatggt cgtgaagggt tagaaactgc tttggaaaaa
    20341 gagtttgatt taatcctgct tgacttaatg ttaccagaga tggatggttt tgaagtgacc
    20401 cgtcgtttgc aaaccgaaaa aacaacgtat atcatgatga tgactgcgcg tgattctatt
    20461 atggatgtgg ttgcaggttt agaccgcggt gcagacgact atattgttaa accgtttgcc
    20521 attgaagaac tacttgcccg tattcatgct attttccgcc gtcaagatat tgaatctgag
    20581 aagaaagtgc ctagtcaagg catttatcga gatctagttt taaatccaca aaaccgttca
    20641 gttaatcgtg gcgacgatga gatttctctc actaaacgtg aatatgattt gcttaatatt
    20701 ttgatgacta atatgaatcg tgtcatgaca cgtgaagaat tattgtcaaa tgtttggaaa
    20761 tatgatgaag ccgttgagac taatgttgta gatgtctata ttcgttatct ccgcggcaaa
    20821 attgacattc caggcaagga atcttatatc caaacagtgc gtggcatggg atacgttatt
    20881 cgtgagaaat aagtcatatg gaaaatcaga aacaaaaaca gaagaaatat aaaaattcgt
    20941 taccaaaacg actatctaat atcttttttg ttcttttttt ctgcattttc tctgccttta
    21001 cactgattgc ctatagttca acaaactatt tcttattgaa gaaagaaaag cagtcagtct
    21061 ttcaagctgt aaatattgtt agagttcgtc tttctgaggt ggactctaat tttacattag
    21121 agaacttagc agaagttttg tacaaaaacg ataaaacaca tctgagaatt gatgacagaa
    21181 agggcagtcg agtcattagg agtgagcgcg atatcacaaa tactctagat gcgaatcaag
    21241 atatttatgt ctataacatt gataagcaga tgatttttac cacagataac gaagaatcat
    21301 ctcctggctt gcatggtcct atcggtcggg tgtatcacga ccatatcgaa gatcagtatc
    21361 gtgggttttc catgacacaa aaggtatatt ctaatcggac tggaaaattt gtgggctatg
    21421 ttcaagtctt tcatgattta ggcaattatt atgtcattag agcaagactg ctgttttggc
    21481 tactagttgt tgagttattt ggcacaagct tagcctattt aatcatctta attactacgc
    21541 ggcgcttttt gaagccttta cacaatttac atggagtaat gcgtaatatc tctgaaaatc
    21601 ctaataattt aaacctgcgc tcagatattt cgtcaggaga tgaaatcgaa gaattgtctg
    21661 ttatctttga caatatgtta gacaaattgg agacacatac taagttgcaa tcacgtttta
    21721 ttagtgatgt cagccatgaa ttacgaacac cggttgcgat tattaaaggg catattggtc
    21781 tcttacaacg ttggggtaaa gatgacagtg atattcttga agaaagtttg acagcaacag
    21841 cgcatgaggc tgaccgtatg gcaatcatga ttaatgatat gttagacatg attcgcgtac
    21901 aaggctcatt tgagggacat caaaatgata tgacagtttt ggaagattct attgaaactg
    21961 ttgttggtaa ttttagagtt ttaagagaag attttatctt tacatggcag tcagaaaatc
    22021 caaaaacgat agcccgtatt tataaaaatc attttgagca ggctttgatg attcttattg
    22081 ataatgctgt gaagtattcc cgtaaagaaa agaaaatcgc gattaacctt tcagtgactg
    22141 gcaaacaaga agctattgtt agagttcaag ataaaggcga aggaatttct aaagaagata
    22201 ttgaacatat ctttgaacgc ttttatagaa cggataaatc acgtaatcga acaagtactc
    22261 aggctggatt agggattggc ttgtctattc tcaagcaaat tgtagatggg tatcatttac
    22321 agatgaaggt tgaaagtgaa ttaaatgaag gttcagtgtt tatcttacat attcctttgg
    22381 cccagtctaa agagagttag agtagcttaa ggcattgtta atgtgacagg tatagtatgt
    22441 aatcattaga taatagtagc agtcttttgc cacaactggt tagaagctga tgctattttt
    22501 ttagcaatga tggatgacat gaaagaaata ctgtcaatcg cgatgccttt tgttatcctt
    22561 gataatatgg taaaatagtg tattagatta atttgataga attggggtgt ttgcgtgcgt
    22621 tgtccaaaat gtaattacca taaatcgagt gttgttgaca gtagacaagc tgaagacggc
    22681 aacaccattc gtcgtcgaag agaatgtgaa cagtgtcata ctcgctttac tacctttgag
    22741 cgtgttgaag aactcccttt attagtcatc aaaaaagatg gcacacgaga gcaattttct
    22801 agagataaaa tattaaatgg ggttgtccaa agtgcgcaaa aacgacctgt ctcgagcaca
    22861 gatattgaaa atgtcatttc tcgtattgaa caagaagttc gaacaaccta cgaaaatgaa
    22921 gtgtcaagta ctgctattgg taatttagtg atggatgagt tggccgaact tgatgaaatc
    22981 acatatgtac gctttgctag tgtctacaag agcttcaagg acgttgatga aatcgaagaa
    23041 cttttgcaac aaattacgaa tcgcgtacgc ggaaagaaaa aacgtttaaa taatgatgaa
    23101 accaattgat acttttacat atctaaaacg caacaaagta actcttgatt cggtgacttt
    23161 aatacagttg tactttccaa ttattggaag tgacgcagtc tctatctatc aatactttat
    23221 tcactttttt gacgatggtt tacaacgtca taaattcagc gatattttaa atcacctaca
    23281 gtttggtatg aaacgttttg aagatgctct tgccatattg acagcaatgg aattagtgag
    23341 tgtgtatcag ctatcagata catatctcat tacgttgcac cagccgctta gccgtgactt
    23401 gttttttcaa catccagctt attctcgatt attagaacaa aagattggag aggtagctgt
    23461 ctctgagttg caggtaactg tcccaagtca agcaagaaat atttctaagc gattttcgga
    23521 tatttttggg gtgcaaggag atttgacgaa cgtacctcaa aagccacaaa agaattttga
    23581 cctttcaagt ttccagcaat taatggttcg tgatgggtta caatttgaag ataatcaaaa
    23641 agatatcatc agcctttaca gtattgctga acagtatgat atgacgtggt ttgatactta
    23701 tcaaatagct aaggcgacgg ctgttaatgg gaaaattcga cctgaacgct tattggctaa
    23761 aaaaaatcaa agcatgacaa aaccaagcaa agaaaatttt tcacaggctg agcaaattat
    23821 tttgcgagaa gcaaagcaag acagtgcttt agtctttttg gaaaaaatta aaaaggctag
    23881 acgtgcaacg attactaagg atgaacgcat tttattgcaa acattggcca aaatgaattt
    23941 tcttgatgat gttatcaatg tcatggtgct ttataccttt aataagacaa aatctgctaa
    24001 tttacagaag agttatgtct taaaaatggc caatgatttt gcctaccaaa aggtgtcaac
    24061 ggctgaagaa gctatagtgg ttttgcgagc cttcacggat cgtcaaagtc gtcggcaatc
    24121 aaaagtaaaa acaagtcaaa gcaatgtgcc taagtggagc aatcctgatt accaagagac
    24181 aacaagtcaa gaagagcagg cgaaactaga tcagttcaaa caggctgctt tgaagaggtt
    24241 agaaaaccta ggaaaagggg gtgactagat ggaaaagatt ggagaaacca tggcgaaatt
    24301 gggtcaaaac actcgtgtta atagcgacca gttgattcaa accattttag cggatcctga
    24361 agtagcaagc ttcatcagtc aacatcactt atctcaagag caaattaacc ttagcttatc
    24421 taagttcaat caatttttgg tggaacgcca gaagtatcaa ctcaaagatc cctcttatat
    24481 tgctaagggc tatcagccta ttctagctat gaatgagggc tacgctgatg tgtcttattt
    24541 ggaaaccaaa gaattggtag aagctcaaaa acaggcagct atctctgaac ggattcaact
    24601 ggtcagtttg ccaaaatcct atcgccatat tcatttacca gatattgatg ttaataatgc
    24661 gagccgtatg gaagctttct ctgctatttt agattttgtg gaacaatacc catcagcaga
    24721 gcaaaaaggc ttatatttgt acggagatat gggaattggc aagtcttact tgttggctgc
    24781 tatggcccat gaattgtctg agaaaaaagg tgtgtcaacg acccttctac attttccgag
    24841 ctttgccatt gatgtaaaaa atgccatttc aaacggctct gttaaagaag aaatcgatgc
    24901 ggtcaaaaat gtaccagtgc tcattttaga tgatattggt gcagagcaag ccacttcttg
    24961 ggtccgtgat gaggtgttgc aggtgatttt acaataccgt atgttagagg aattgccaac
    25021 cttctttacc tctaattaca gctttgcgga tttggagcga aaatgggcaa ccatcaaagg
    25081 aagtgacgaa acttggcagg ccaaacgtgt catggaacgc gtgcgttatt tggctagaga
    25141 gtttcattta gaaggggcta accgccgtta agtttgagat agcaatcaag aagaatagct
    25201 acacgtatga gtgagtgaaa gaaaaggaga atcatggttt tacctaccgt tgccattgtg
    25261 ggccgtccaa atgtgggcaa gtccacctta tttaatcgaa tcgcaggaga gcgcatttca
    25321 attgtagaag acgttgaagg ggttactcgt gaccgtattt atgctactgg ggagtggctt
    25381 aaccgccaat tctcattgat tgatacaggt ggtattgatg acgttgatgc cccgtttatg
    25441 gagcaaatca agcaccaggc tcaaattgct atggaagaag cggacgttat tgtctttgtc
    25501 gtttctggta aagaaggggt gaccgacgct gacgaatatg tgtctaaaat tctgtatcga
    25561 accaataccc cagtcatttt agctgttaat aaggttgata atcctgaaat gcgtaatgac
    25621 atttatgatt tttattcact tggtttaggg gatccctatc cagtttcttc tgttcatggt
    25681 atcggaacag gggatgtctt ggatgagatc gttgaaaact tgccagtaga agaggctgaa
    25741 gaaaatgatg atattattcg cttcagttta atcggtcgtc ctaatgttgg gaaatctagc
    25801 ttgattaacg ctattttagg tgaggaccgt gttatcgcta gccctgtcgc agggacaaca
    25861 cgtgacgcta ttgacacgca ttttacggat gctgacggtc aagaatttac catgattgac
    25921 acggctggaa tgcgcaaatc tggcaagatt tacgaaaata cagagaaata ttctgtcatg
    25981 cgtgccatgc gtgccattga ccgctccgat gtggtcttga tggttatcaa tgctgaagaa
    26041 ggtatccgtg aatacgacaa gcgtatcgct ggctttgcac acgaagctgg taaaggaatg
    26101 attatcgtgg ttaacaaatg ggataccatt gacaaggata atcataccgt tgctaagtgg
    26161 gaagccgata ttcgtgacca attccaattt ttgacatacg ctccgattat ttttgtgtca
    26221 gctttgacga agcagcgcct caacaaattg cctgacttga ttaaacgtat cagtgagagt
    26281 cagaataaac gtatcccatc tgctgtcctc aatgatgtta tcatggatgc tattgctatt
    26341 aatccaacgc caacggataa gggtaaacgt ttgaaaattt tctatgccac tcaagtgtca
    26401 gtaaagccac caacatttgt cgtctttgtt aacgaagaag aattgatgca cttctcttat
    26461 ctacgcttct tggaaaacca gattcgcgca gcctttacct ttgaagggac accgattcat
    26521 ctgattgctc gcaaacggaa ataagaaatg aacatcaact aaaatgctga cctaagaaat
    26581 aatctcatca ttgataagga ctgtgttgtg tatgttacag gagacagtcc tttttataat
    26641 acctttaagg gtgtagacgg aagattagta gcaaacaaag aaaaaggatt ttgacaagaa
    26701 atatcagtcg gtttttgcta taatgaaagg ataagcaaaa aggtggaaaa atgatggcta
    26761 gattaattcc aggacgagta aggaatgaag gtattaagct ttatgagcaa ggtttagtct
    26821 cattccaaga tgataataaa ggcattcttc aaatagaagt ggagacttat caggttcaat
    26881 atggagcaga tgatgaggat atcacttgtc aatgcgacac atttcatatg aaacattatt
    26941 gcaagcatat tgcagcagtc gagtattttt taaaaaatga tcaaaaaggt aagctttttt
    27001 taaaacagtt aacaaatcaa acaaaaatca aagaaacaac aaagaaaatg acatcttttg
    27061 gtagtctctt tttagatagc ttagccatga atgaagatga ttctgttaaa tacaggctgt
    27121 ctgctcttgg tagtcgaagc cctttttcgt cagattactg gtggagtctg aaaatcaatc
    27181 gtttaccaga tgatcgttct tatgtgattc gagatattaa aggctttttg cagcttatta
    27241 aaaaagaagg cttttatcag attggaaaaa attattttga acaactttca tggctgcagt
    27301 ttgacccatc cagtcaagac cttattgagt ttttatggcg cttagcatct aatactgaca
    27361 aaggggacaa cgagaacatt tttccaaatc atgcaaggca tttgcgctta cctagtggtt
    27421 tttttgaaga aggtattcac tatttgacgt cactttatga ctttactttt gaggggcctt
    27481 ctcaaactta tcatcatctt tttgtccgtt ctttggaagc agaggcaggc ctttatgagt
    27541 ttaaagtgga agtgcatcgc aaatctattg aattgcaaat tgctgaaaaa aatgtgcaat
    27601 acctctttga taatgactat cttctttatc aagatacctt ttatcacttg acgttaaagc
    27661 aacgaaaaat ggttcaggct attcgtagtc tgcccattga ggccgacttg gctaaacata
    27721 ttcattttga tttggatgat catgcgaaat tagctgcaag cttatcagat tttaaacaga
    27781 tcggtctagt agaggctcct aaaagctttg ctattagaga ctttgaggtg acttttcaat
    27841 ttgatttgtt aaatcgagat gaaatcagtt gtcagcttat gtttgattat ggcaattacc
    27901 aagttagtga caaagcaagt ttagaagcac ttccttttgc tagtcattta aaaaaagaag
    27961 aaaaaatcaa tcgttcttta cttgcttttg gcttctcgcc acaattttac tcaaaaaagc
    28021 gactaacaag tgcaaaagag ctctatactt tttttgaaga aacagttcca tgttttgagc
    28081 gcttaggtaa tgttgcgttg agtacagcta tccaagcatt acaagtgaaa gaaatgccca
    28141 agatcgctat tagacgcaat cagggattac tagatatttc gtttgatttt tcaaccatta
    28201 ttgaaaatga tattgaccaa gctgttacag ctctttttca aaataatcct tattttgtga
    28261 gtcaaacagg tcaattagtg gtttttgatg atgaaactca aaaagttagc aaaagtttac
    28321 aagaattaag agcaagacag ctaaaaaatg gtcacttgca actggatggc attagagctc
    28381 tacaagtatc caaacttttt gaaggcatga catctgttca tttttcaaag gaattagaag
    28441 agttagccta tcatttgcag catcctgaaa ctttttcaat aaaaccgtta ccagttaagg
    28501 ctcaaatgcg agattatcaa cgcaacggtg ttcagtggtt atcaatgctc aatcattatg
    28561 gctttggtgg cattttagca gatgacatgg gacttggaaa aactctccag acacttgctt
    28621 ttttagctag tcacctgaaa tcagatagta aagtgctcat tttatctcca tctagtctta
    28681 tctataattg gtttgatgaa tgtcaaaaat tcacacctca gctagatgtt gtagtttctt
    28741 acggacttaa acaaatcaga gatcaaatca tcgaagaagg tcatcaaata accataacca
    28801 gctattcctc ctttagacag gattttgaga cctatcaagc gtttcactac gattatctta
    28861 tcttagatga agctcaagtg ataaaaaatg cgcaaactaa gatttcacac tgtttacgtg
    28921 cgtttaatac agccaattgt tttgcgcttt cgggcactcc tattgaaaat aaaatgcttg
    28981 aaatttggtc cattttccaa attgttttgc cagggttgct accgactaaa aaagagtttt
    29041 taaaattaac agctgagcaa gtatcgcgct atatcaaacc ctttgtgatg cgtcgtaaaa
    29101 aagaagatgt cctaccagaa ttacctgatt taattgaaac taattattca aacgagatga
    29161 cagatgaaca aaaagctatt tacttagcgc agttaaggca gatgaaagat caaattcgaa
    29221 attctagtga tgtggatatt agccgtcaaa aaattgagat tttatctggt atcactcgat
    29281 tgaggcaaat ctgtgacact ccaagtctct ttatggatta ccaaggcaaa agtggtaagc
    29341 tagatagttt gcgaatctta ttgacacaaa ttaaagaaaa tggtcatcgg gctttgattt
    29401 tttctcaatt tagagggatg ctggacttgg ccaagcaaga gatgactgct ctaggattga
    29461 cctcttatca aatgaccgga tctacccctg ccaatgaacg tcaagaaatg actagagcct
    29521 ttaataacgg ttccaaggat gcttttttga tttccctcaa ggcaggtgga gttggaatca
    29581 accttacggg agcagacaca gtgattttaa tcgatttatg gtggaatcct gctgttgaaa
    29641 tgcaggcaat cagtcgcgcc taccgtattg gtcaaaaaga aaacgttgaa gtatatcgct
    29701 taatcactcg agggacgatt gaagaaaaga ttttagagct tcaagaaagt aagcgcaatt
    29761 tggtgactac agttttggat ggcaatgaaa accgagcaag tatgtccatt gaagaaataa
    29821 aagaaatttt ggggctaaac actcattaat cttccttgat tttagtgaca ttaaaaaccc
    29881 tttaaagaag atgacaccaa aagcacctta tataacctcg ttaaaacgct tctttttgaa
    29941 atatctttat tgatatagcg attgtcattt gctagtaatg attaaaacat ttagaaaaag
    30001 acaagtcata gaatcatcaa ggaatgcttt gaaaaataac agtatttaag ttataataga
    30061 ggatgctaca agaagggaga atcaaaaaag gatggtaaaa aatcagtttc ctttagttgc
    30121 agatggcatt gctattagtg atccagcaaa gcaaatggct ctgtatgaaa atgaagatct
    30181 cattaccaac atccgtggct attaccagga caaggagtat gatgatattg ctagaaatga
    30241 ggagtttact gctaaagcaa cttctcggca ggccccttct agtaagcgct tttgtagcaa
    30301 tgatgaaaaa caccattatg tcaaagaggc gcgtcaaaaa gcaaagcaag atctcaagga
    30361 aaaaagacag gcttatcttg ccaaagaaat ggcctatgtt cctaaacagg tttctaaaaa
    30421 gcaacagcct gcggattctt ctccttctca aaagcaagca acaacagaaa tgagtcgttt
    30481 tacaaaaaag cttcatcaag acaactatat tttagctgag ttgccaaaag aatataaaga
    30541 gccaaaaaat cttcctcaac aaggaactac aaaaaaaaat aattacgatt ttttaaaatc
    30601 cagtcaaatt tacaataata aagaaatgag acaacaacga gaaaaaacaa ttgcgcaaga
    30661 gcttaatctt agtaggtttg aggatcttcc ttaataaatc tactcagcgt atgcagatga
    30721 caaaggagtt aagatgtcaa aaacgtatca ttttatcgga attaaagggt caggaatgag
    30781 tgccttggcc cttatgttgc atcaaatggg gcataaggtg caaggtagtg atgttgaaaa
    30841 atactatttc actcaaagag gattggaaca agcgggcatc actatcttac cttttagcga
    30901 ggacaacatt acgccagata tggaacttat cgttgggaat gcctttcgtg aaaacaacaa
    30961 agaagtggcc tatgctttga gacaccagat tcctttcaaa cgttatcatg actttttagg
    31021 tgattttatg aaatcattta tcagttttgc agtagcaggt gctcatggta aaacttctac
    31081 aacgggttta ttgtcacatg tcttgaaaaa tatcacagac acatcttatt taattggtga
    31141 tggtactggg cgtggatctg ctaatgcaca gtattttgtt tttgaatcag acgaatacga
    31201 acgccatttt atgccttatc acccagaata ctctatcatt actaatattg attttgacca
    31261 tcctgattat ttcacaggaa ttgctgatgt gcgaaacgct ttcaacgatt atgctaagca
    31321 ggtcaagaaa gctttgtttg tttatggtga agatgacgaa ctaaaaaaga tcgaagcacc
    31381 tgcacctatt tactattatg gttttgaaga gggcaatgat tttatcgctt acgacattac
    31441 gcgtactacg aacggatcgg actttaaggt aaaacaccaa ggagaggtca ttggtcagtt
    31501 tcacgtacct gcttatggaa aacataacat tttaaacgct acagcagtaa ttgcaaactt
    31561 gtttgtagcc ggcattgata cggctctagt ggcagaccat cttaaaactt tttcaggtgt
    31621 gaaacgtcgt tttacagaga aaattatcaa tgacactatt attattgatg actttgctca
    31681 tcatccaacc gaaattgtgg caacaattga tgcggctaga caaaaatacc caagtaaaga
    31741 aattgtagca atatttcagc cacatacttt tactcgcact attgccttat tagaggattt
    31801 tgcttgtgct ttaaatgaag ctgacagtgt ttatttagcc cagatttatg gttctgcacg
    31861 tgaagtagat aaaggtgaag ttaaggttga agatttggct gctaagatta tcaaaccatc
    31921 acaagtagta acggtggaaa atgtctctcc tcttcttgat cacgataatg ctgtttatgt
    31981 ttttatggga gctggagata ttcagctata tgaacattct tttgaggaat tacttgcaaa
    32041 tttaactaaa aataaccaat aacatgtcct gccttaggca ggttttctca ttctaaagga
    32101 gtttgatatg ttgattagac aggttcaagg atccgatttg gaagttattg ctaccattga
    32161 aagtgacaat ttttcaccac aagaagcaac gacaagagca gttcttgaag agcgcatacg
    32221 gctgatacca gatacttttt tagtggcact tatcgatcag gaaattgttg gctatatcga
    32281 aggtccagtt gtcactactc ctattttgga ggattctttg ttccacgggg tgactaaaaa
    32341 tcctaaaact ggaggctata ttgctattac tagtttgtcc atagctaaac attttcagca
    32401 acaaggggtt gggacagctt tgttggcggc actaaaagat ttagtagtag cgcagcaaag
    32461 aacaggtctt attttgactt gtcatgatta cttgatttcc tattatgaaa tgaatgggtt
    32521 tattaaccaa ggaatttcag agtctcaaca tggtggaacc ctttggtacc aaatgatttg
    32581 gaaactttag aatcacagct gattcaactg ttggtttgtt aaaaataagt aacaaatgca
    32641 tcatttaatt gcaattagga tacttttaag ttataatggc ggttgattat atttaggagg
    32701 attggctttg accgatttta aggataaaga ccaacaagat cagcagcgaa gctttaaaga
    32761 gcagattctt gctgagttgg aaaaagcaaa tcaaatcaga aaagaaaaag aggaagaact
    32821 ttttcaaaaa gagttggaag ctaaagaagc agctaggaga acggcccagc tatatgctga
    32881 atataaaaga caagatgctt ttcaaaaaga gtctatagca cataacaata agacagctaa
    32941 acactttcaa gctataaaag gtgcggtaat gacttcagaa gcgcttaaac cgactttact
    33001 ttctgaaaaa gaaaactcat ctttaaaaac gacaaataag agagtcgtgc aggcaaatga
    33061 gcttcaagag actgcctcta aagaatctca agtaccgtta actattgaga aaggtcattc
    33121 agtgagacga aaattaagca aacgccaaca gactgagcga gctgctaaaa agatttcaac
    33181 cgttttgatt agttctatta ttataaccct tttggctgtt actctagcag gagcaggcta
    33241 tgtttatagt gctttaaatc ctgttgataa aaatagtgat gcctttgttc aagttgagat
    33301 accatctggg tcaggcaata aattgattgg tcaaattctt caaaaaaaag gtttaatcaa
    33361 gaatagcact gtttttagtt tttatacaaa atttaaaaac tttacaaatt ttcagagcgg
    33421 gtattacaat ctgcaaaaaa gtatgagtct agaagaaatt gctagtgctt tacaagaagg
    33481 tggtacagca gaacctacca agccatctct tgggaagatc ttgattccag aaggatacac
    33541 gattaaacaa atagctaaag ctgttgagca taatagcaag ggaaagacca aaaaagctaa
    33601 aacacctttt aacgagaagg attttttgga tttagtcaca gatgaggctt ttattcaaga
    33661 tatggtaaaa agatatccaa aattattagc aactatccca actaaagaaa aagctattta
    33721 ccgtttggaa ggttaccttt tcccagcaac ctataactat tacaaagaaa ctaccatgag
    33781 agaacttgta gaggacatgc tggcagctat ggatgctact ttggtaccct attatgataa
    33841 aattgctgct agtggtaaga cagtcaacga ggtattgacc ttggcctctt tggttgaaaa
    33901 agaaggttca acagacgatg acagacgtca aattgcaagt gtcttttata accgccttaa
    33961 tagcggaatg gcactacaat ctaatatagc tattttgtat gcgatgggga aacttggtga
    34021 gaaaacaacc ttggctgagg atgctactat tgacaccacc attaattctc cttataatat
    34081 ttataccaat acaggtctga tgcctggtcc agttgctagc tcgggggttt ctgcaattga
    34141 agcaacccta aatccagcct caacggatta tttatacttt gtggccaatg tccatactgg
    34201 tgaagtttac tatgcaaaaa catttgaaga acactctgca aatgttgaaa aatatgtgaa
    34261 tagtcaaatt cagtaaacac aaacatggtg cttaagcacc atgtttgtgt ttctgattcg
    34321 ttcaaaaaag aaaagagaga aaacaattat ggcagaaaaa acgtatccga tgaccctaac
    34381 ggaaaaagaa caacttgaaa aagaacttga agaattaaaa cttgtgcgtc gtccagaaat
    34441 agtggagcgt attaaaattg cacgttcgta tggagatctt tcagagaact ctgaatacga
    34501 tgccgcaaaa gatgagcaag cttttgttga aggtcaaatt tcaacgcttg aaactaagat
    34561 tcgctatgct gaaatcattg acagtgatgc tgttgcaaaa gatgaagtcg ctatcggaaa
    34621 aacagttatc gttcaagaag ttggaacaac agataaagat acctatcata ttgtaggtgc
    34681 tgctggagca gatatcttct caggaaaaat ttcaaatgaa agcccgattg ctcaagcttt
    34741 gattggtaaa aaaacaggtg acaaggttag aattgaatca ccagcagcta cttatgatgt
    34801 tgaaatcatc agcgttgaaa agacaaatta atcagatgaa gccattattt gcctaaccac
    34861 taagtgtttt ggttgctaat ggcttttcta gtatcttcaa atgaggttga ctgttaggaa
    34921 ggtcaagtat taatcgcatt gattatgagc caagaggact atagatcaaa gatcgttttc
    34981 gatggttatt taatgccaaa agaaaagacc agcaccttaa tgtactgacc ccaaaaagtt
    35041 ggacactata ttttaaggaa gtgtatgatc taaggaaact gacactaatg attgaccgtt
    35101 ttgcaagact ttattaatga tttcttgctt tattttaaat gaataagtct gatacgtttc
    35161 cttccttaac actatcgact ccatactgac ccatcagtca aacggtatat ctcaaacgac
    35221 tacggtttac tccaaattga ttggctagtt gaggccaaga aacacctgat tttttttaat
    35281 gctaaatttc tagcttatct tcatagctta atttcataca aaaacacccc aatcgttaga
    35341 tttttttgat atgtcccctt tcactagatg agtccagtaa aagggggcat atcgtttttt
    35401 gtctaacttt tggggtgcag ttcataattg tgctaggttt tttgtctttc ttaacgcttt
    35461 cgctgttttc cagcattacg attggatttt atttttttag gcaagttagc ctgttccatg
    35521 ttttgtgaag gcgtaacgtc cttacgagaa gaagttgatt ggtatgcttt aggcgggttt
    35581 ttagcgtatt cttctttaat ttgcttatgc aatcgaggtt ttaagaggta ggttgtgatt
    35641 aactgttgaa taatactaaa gaaaccacca actagccagt aaagaccaac accagcaggg
    35701 agtgagaagg acatgaagat catcatgata ggcatggtgt acatcatcgt cttcatttgc
    35761 tcacgttgtt cttctgaaac ggccatcatt gataaccatg attggaagaa gtaaagagca
    35821 gcgataattg ctgttaacac taaactacgg ctaccaagat caatacccat aaaggtgctt
    35881 gttgatactc ctttagtgta ttgagcggca aaatacatgg cagaaaagaa tggcatctgg
    35941 atgagaagag gtagacatcc aatacctcca agaggattaa tcccatgagc acgctgagca
    36001 gccattaatt ctgtttgggc agccattttt tcttcctgac tacttgcctg tttgatacgt
    36061 ttgttgattg gttcaaaaac aggtttcaag aaggccattt tctcagattg ataacttgct
    36121 ttccaagatt gatatagacc tagtggtaag ataagtgttc taacaataat ggttacgatg
    36181 atgatagcca gtccataacc tagtccagca ttgtttgcaa agtaatcgat aaagtatgac
    36241 atgggttttc caagaaattc ccaaatcata cctttgggat ttccgtgagc gtctcttccc
    36301 acacagcctg tcaaggtgaa tagaattgac agtgctagtc ctgaaaagag gatgcgattt
    36361 aatgttagtt tcaatgtgtt tatcctttaa ttttaatata cttatctatt ttacagtttt
    36421 tttcagaaat tcacaatagt tctgaaggcc gatttttact tgcctatttg aaagtcctga
    36481 aagtcttcaa aattagccat ggtgacatcc acataagtga cttttgccca tttggatggt
    36541 cctttacgaa cttcttgaat aaatgtggct attttatctg aatcttttga ttgggccagg
    36601 atttcaacgg tcccatcgtt gttattccag acacgaccat aaatatctcc tatatctaga
    36661 gctaaagtat gggtagcgta tcgaaaacca acaccttgta cacgtccaga aacaattaaa
    36721 cgtacttttt gcataagaag ggcctccttg cttttattat agcacatact tagcaggaaa
    36781 tctctttagc taattgtcta cttcctgtca cttttactaa agacaagaat acaaaaatga
    36841 gatagttagt agtactaaca gtaaaagagg tttgtctatt ttaaaaatag tatcaaaaaa
    36901 aggtctttta ggtttaaaac attgaactgc ccccaaattg accatctaag aagttgatgc
    36961 tttcaaatgc tgtcgcgtat ggtataatag gtgcatggtt attacatcaa aaacaaatca
    37021 actaattaag caaacaaaaa aattattgca aaaaaagcat cgaaaacagt cctatttgat
    37081 tgagggctgg cacctttttg aagaagccca aaaatcagga caagtttttc gccacatctt
    37141 tgttttagag gaaatggtgg agcgtctagc aggtgagcaa gaactcgtta ttgttagccc
    37201 acaagtgctc aaggagttga ccgactctcc tagtccacaa ggcattgttg ctgaggtgga
    37261 gatacctaag ttagcctttc catcagatta caaagggaaa tatttggttt tagaggacgt
    37321 ccaagaccca ggaaatcttg ggaccatcat tcgtacagca gacgctgcac ggtttgatgg
    37381 ggtctttctt tctgaaaaat cagcagatat ttacaaccaa aaaaccctac gttccatgca
    37441 aggtagccat tttcatttac caatttggcg aacagatgtc tatcagcttt gtcgagagct
    37501 ccaagagtat aaaactccca tcctagcaac aactctgtca aagaaatctg ttgactataa
    37561 aagcctcaca catcatgaaa ggttggcttt ggtgttggga aatgaaggcc agggcattag
    37621 tgctgaaatg gcagccctag cagaccaact ggttcatatt acaatgcctg gtcaggcgga
    37681 aagtttgaat gttgccgtag cagcaggaat cctcattttc agcttgattt aagctaatat
    37741 gatataataa ccaagtgggg tgattatgtt atgcattata cagaagataa agaatatatg
    37801 gaacacgtcg gtcatttgat cgctcatcct cgctttcaaa aattgagtca tattgttcag
    37861 caccaacatt caacgcgtct tgaacattct atcaatgttt cttattccag ttataaatta
    37921 gccaagcgat ttggttggga tgctaaaagt acagccagag ggggcttact tcatgatttc
    37981 ttttattatg attggcgcgt gaccaagttt aataagggtc atgcctgggt tcatcctcgt
    38041 attgctgtac gaaatgcgaa aaagttaaca gaactcaata aaaaagaaga ggatatcatt
    38101 ctaaaacaca tgtggggagc gaccattgct tttccgcgtt acaaggaaag ttatattgtg
    38161 acaatggtag ataaatactg ggctgtcaag gaagcagtga cacctttaag acaaaaatgg
    38221 tctaatcgac ggtttcttcg ccgcaagacc ctccaaagtc ataatcgtta agtaaaggag
    38281 ttattatgaa cgatcacgtt atttatacac aatcagatgt gggattaaat cagttttttg
    38341 ctaaaattta tagtctagtg ggaatgggag tgggtttatc tgcttttgtt tcttatttga
    38401 tgctttatcc atttagagaa aatttaatct ctattcttgt taatcagccg atgatttact
    38461 atggtgctgc gattattgaa cttattttag tttttgtagc cagtggtgca gcacgaaaaa
    38521 acacaccagc tgcattgccc atttttctta tctattctgc gctaaatggc tttaccctaa
    38581 gctttattat tgtggcatac gctcaaacga cagtttttca agcatttctt tcttcagcag
    38641 ctgtgttttt tgccatgtca atcattggtg tcaagactaa acgggatatg tcaggtctta
    38701 gaaaggctat gtttgctgct ttgattggtg ttgttgtggc tagtctgatt aatcttttta
    38761 ttggtagtgg gatgatgagt tatgtcatta gtgtgatttc cgtgctaatt ttctcaggtc
    38821 tcatcgcatc agataatcaa atgattaagc gcgtttatca agcaacaaat ggccaggttg
    38881 gtgatggctg ggctgttgct atggccttaa gcttgtattt agattttatt aacttattta
    38941 tcagccttct tcgtattttt ggaagaaatg attaacatta aaaagtgagt ataactactc
    39001 actttttaat gttaacagga taagtaaact gtaaggaaga aattcgagat agatataaga
    39061 tgaggctgct aaaagaccta gtgttaattt gtaaactctt tctttttttg gtatactatt
    39121 aaggattaaa tacgaggaga atatagatag atatgtcaac agctatttgg attttattac
    39181 ttattgttgc ccttggggta ggtgtttttg gtgggatttt tattgcccgt aaacaaattg
    39241 aaaaagaaat tggtgaacac ccacgtttaa caccagaagc tatccgtgaa atgatgagcc
    39301 agatggggca aaaaccaagt gaagcaaaaa tccaacaaac ttatcgtaat atcatcaaac
    39361 aatcaaaagc agccgtatcc aaaggtaaaa aataaaagct aattgttata ggggaaacct
    39421 ttgtgatagt tagaagtgaa cagtctttga tctcaatgtt cagtagcact tgtcttatca
    39481 gttagatttg tcaactgttc agggtttaaa aagaaactca gttggctatt tccaactgag
    39541 tttttcatat cgtcagtaag gaagtaaaca taatggatac aagaccaatt gggtttttag
    39601 attctggtgt gggaggtctg accgtcgttt gtgaattgat tcgtcagctc cctcatgaga
    39661 agattgtata tattggtgat tcagcaagag ccccctatgg tcctagaccc aaaaaacaaa
    39721 ttaaggagta tacttgggag ctagtcaatt ttttgctgac tcaaaatgtt aaaatgattg
    39781 tttttgcatg caataccgca acagcggtgg cttgggaaga agtaaaagca gctttagata
    39841 ttcctgtttt aggggttgtc ttaccggggg caagcgcagc tattaaatca acgacaaaag
    39901 gccaggttgg ggtcatcgga accccaatga cagtggcttc agacatttat cgcaaaaaaa
    39961 tccagctatt agcaccatct gttcaagtaa ggagtcttgc ttgcccgaag tttgtaccga
    40021 ttgtggaatc aaatgagatg tgttcgagta tagctaaaaa aatagtttat gacagtctat
    40081 caccattagt cggtaaaata gatacccttg tactaggatg tactcactat cccttgttac
    40141 gaccaattat ccaaaatgtt atggggccat ctgttaagct gattgacagt ggagcagaat
    40201 gcgtccgaga tatctctgtc ttattaaact attttgacat taacggtaat tatcatcaaa
    40261 aagctgtcaa acaccgcttt tttacaacag caaatcctga gatttttcaa gaaattgcct
    40321 cgatttggct gaaacaaaaa ataaatgtgg agcatgtcac cttatgagtg aaaaaattta
    40381 cgaatacaaa gatgaaaata actggtttat cggaaagatg actggtcata atttaatatc
    40441 gggctgggga gtcaaacaca cgacaatcaa aaaaatagat gaccttttag acggtattgc
    40501 cgcaacgtta gattgggaaa atcctaaagg ttacgatgtt tcggtggtgc gttatcagtc
    40561 tcctttatct ctcatcacct ttattatcga catgataaat caagagaccc agcgtaagat
    40621 taaggtaact ccccatgcag gcacgatttt attaatggaa aatgcgaaac tgcttgcagt
    40681 ttatctccca gaaggtggag tttcaacggc tacatttttt gcgactagtg aacagggatt
    40741 tggggatatt attttaatcg ctacacgaaa cgagggaaaa accaaagagt tccgcaatct
    40801 gtttggccag ctaggttatc gtgtggaaaa tctcaatgat tatccagaat tgccagaagt
    40861 agcagaaact ggtactactt ttgaagaaaa tgctcgcttg aaagcagaaa cgatttcacg
    40921 cctcacagga aaaatggtat tagcagacga ttcaggacta aaagttgatg ccttgggtgg
    40981 tttacctggg gtttggtcag ctcgtttctc aggaccagat gccacagatg ccaagaataa
    41041 tgccaaactc ttacacgaat tagctatggt gtttgaccaa aagaagcgct cagctcaatt
    41101 tcatacgact ttggtagtgg ctgctcccaa taaagatagt ttggttgtgg aagccgagtg
    41161 gccaggctat attgctacgc agcctaaggg agaaaatggt tttgggtatg accctgtctt
    41221 tatcgtcgga gagactgggc gtcatgctgc agaattagag gctgaccaaa agaatcagtt
    41281 gtcgcataga ggtcaagcag ttcgaaaatt aatggaggtc tttccagcat ggcaagcaaa
    41341 acaatcatag taatgagtga ctctcatggt gaccgagata tcgttcaagc cattaaagac
    41401 aagtatcttg gtcaagttga tgctattttc cataatgggg attctgaatt aaacagctca
    41461 gatcctattt gggctggtat ttatgtcgtt ggcggtaatt gtgactatga tacaggttac
    41521 cctgatcgtt tagtaactca attaggcaca gttacgattg cccagacaca tggtcacttg
    41581 taccatatta atttcacttg ggataaactg gactactttg ctcaagaagt agcagcagat
    41641 atttgtctat atggacacct acaccgtcca gctgcttggc aagttgggca aaccctcttt
    41701 atgaatcccg gatcagtaac acaacctcgc ggagaaatta acgagaaatt atatgctcgt
    41761 gttgaactaa ctgatactca aattaaagtt gattacttta caagagatca taaactttac
    41821 ccatcactgt ctaaggaatt taaacgatga tcgcaaaaga atttgaaacg tttttaatga
    41881 gtcatttaga taattatctt atcccggaac aagacttggc tatttttatc gacacacata
    41941 atgcagacca tgtgatgtta cttttggtga gcaatggttt ttctagagtt cctgttatca
    42001 cacgggagaa aaaatatgtg ggaaccatta gcatctcaga tattatgatg taccaatcta
    42061 aaaggcagct gacagattgg gaaatgagtc aaacagatat tggcgagatg gttaatacca
    42121 aaattgaaac cattagcatt acttccagcc taacagaaat tatgcacaag ttaattgatt
    42181 tcccattctt accggttgta gatagagcga atcgctttgt tgggattatt acgcgaaaat
    42241 ctatattaaa ggctgtcaac agtcttttac atgattttac agatgattac acgattataa
    42301 aaaaatgaag agttatattg aaccttttat tgctagcaag gctttatctc aaaatagtca
    42361 aaaagcttat cgatacgatt tgcaacagtt ttgtcaactg attggtgaac gtgtcaatca
    42421 agacaaacta ttactttatc aaaatagtat agctaacctc agtttatctg caaaaaaacg
    42481 caaattatca acagctaatc aattcttata ctacttgtat caaataaagt atttaaatag
    42541 ctattttcga ttaacagaca ccatgaaagt gatgcggact gagaagcagc aagctgctat
    42601 tattaatact gatattttct atcaaaaaac cccttttgtg tgggggcaat taatcagctt
    42661 attaatattg gagctgggtt taactccgag tgaagtagct ggtattgaag tagctaactt
    42721 ggatttaaat tttcaaatgt tgacgctaaa gactaaaaaa ggtgtgcgtg tattaccttt
    42781 gtcgcaaata ctaatccctt ttttagaaca acaactcgtt ggcaaagaag cctatctttt
    42841 tgaacatcga ggcatccctt tttcaagaca atggtttttt aatcacctaa aaacctttgt
    42901 tcgaagtatt ggttatgaag gattaactgc tcaaaaatta cgagagcaat ttattttgaa
    42961 agaaaaacta gctggtaaat cgataataga acttagcgat attcttgggc tgaaaagccc
    43021 tgtgacctta gaaaaatact acaaatcata atggatataa aacttaaaga ttttgaaggt
    43081 cctcttgatt tactcttgca cttggtatca cagtacaaag ttgacatcta cgaggttcct
    43141 attgttgagg ttattgagca gtaccttaat tatattgaaa ccttacaggt aatgaaactt
    43201 gaagtggctg gtgactacat gctgatggca agtcagctca tgttaatcaa aagtagaagg
    43261 ctattaccaa aggtggtaga acacatcgaa gaagaagatt tagaacaaga tctgttagaa
    43321 aagatcgaag aatatagtcg ctttaaagca gttagtcaag cgttagcgaa gcaacatgac
    43381 caaagggcaa aatggtattc aaaacctaaa caagaattaa tttttgaaga tgctatctta
    43441 caagaagata aaacggtgat ggatctattt ctagcttttt caaacataat ggcagctaaa
    43501 agagcagttt taaaaaccaa ccatacagtc atcgagcgtg atgattataa gattgaagac
    43561 atgatggctt ctatcaaaca gcgactggaa aaagaaaacg ttattagctt atcagcgatt
    43621 tttgaggaat gccaaacatt aaatgaagtt atcagtatat ttttagctag tctagaattg
    43681 attaagcttc acgtggtatt tgtagagcag ctctctaatt ttggggctat catactacga
    43741 aaggagaaga aatgacgtat ttgtcacaga tagaagcgct gctatttgtt gcaggtgaag
    43801 aagggttaag tttgcgacat ctagcgtcta tgttatcatt aacgccaaca gctcttcaac
    43861 agcaattaga aaaactctct caaaagtatg aaaaagatca acattctagt ctttgtttga
    43921 tagaaacagc taatacctac aggctagtaa ctaaagaagg ttttgcagaa ctcttgcgtg
    43981 cttatgccaa aacaccaatg aaccaaagtc tatctcgtgc gagtttagag gtcttgtcta
    44041 ttgttgcata caaacaacct attacacgaa tagaaattga tgacattcga ggggtaaact
    44101 ccagtggagc tttgagtaaa ttattggcat ttgatctcat tagagaagct ggcaaaaaag
    44161 atgtggttgg gcgtcctcat ttgtatgcga caacagatta ttttctggac tatatgggaa
    44221 ttaatcattt agacgaattg attgaagtat ctgccgttga accagcagat gaagagattg
    44281 ctctttttag gacacaagac tgagttttgc ttgtcatcaa atagcaaatg gcgtttacaa
    44341 aaggatttgt tttaaggcgt tttagttgaa aaaaagcctt aaactaggga caacaaaaag
    44401 ttgctcaaaa gaaagaagaa tataatgcga attaacaaat acattgccca tgcgggtatt
    44461 gccagtcgac gtaaggctga agaattgatt aagcaaggct tagtaacctt aaatggtcag
    44521 gttattacgg atttagcaac cactgtaaaa tcgggagacg tggttgaaat agaaggaagt
    44581 cctatttaca acgaggaaaa ggtttactat ttgcttaata aacctcgtgg cgctatctca
    44641 agtgtgtcag atgacaaagg ccgtaaaacg gtactggatt tactgcctca ggttaaagaa
    44701 cgcatatacc ctgttggacg attggattgg gacacatcag gggtccttat tttaacgaat
    44761 gatggtgatt ttacagatac tatgattcat cctagaaatg aaatagacaa ggtttatctg
    44821 gcgcgtgtca aaggaattgc aacgaaagaa aatcttcgtc ccttaactcg aggaattgtt
    44881 attgatggta aaaagaccaa accagctcgc tacaatattg ttcgtgtaga agctgataag
    44941 aatcgatcga tcgtagaatt gactatccat gaagggcgaa accatcaggt taaaaaaatg
    45001 tttgagtctg tggggctttt ggttgataaa ttatctcgta cacgttttgg gaccgtcgac
    45061 ttaaagggac taagaccagg agaagcaaga cgcttaaata aaaaagaaat aagtcaactg
    45121 cataacttag ctaatacgaa aaaatgatga aaaaactatt aatagtatct gttaaagctt
    45181 atcaaaaata catttctcct ttatcgcctc ctagttgtcg ctacaagcca acctgttcag
    45241 cttatatgtt aactgctatt gaaaaacatg gtactaaggg aatactaatg gggatagcaa
    45301 gaatattaag atgtcaccct tttgttgctg gtggtgtcga tcctgttcca gaagatttta
    45361 gcctaatgag aaataaaaac acgtctaaaa acgctgaaaa ggcatgactt tatcaaaaaa
    45421 tggcttgaca ttgatagctg aagtgttata atggaaaaca agcattcgtt ggtttaaatc
    45481 aaacctgtta tgatttaagt tagcgagtca ccatcaacca cattgtttgc tgagcatgac
    45541 tccgagcagt gtggtttttt aaattaaaaa gagtaaatga gattatgacg acaaaagaac
    45601 ttatcaacaa aaacgacaaa gtaaaaaagg ctcgcaatca tattgtcctg tttcaacccc
    45661 aaattccaca aaatactggt aatattgcta gaacatgtgc tgcaactaat gcacctttac
    45721 acattattaa acccatggga tttcctattg atgaccgcaa aatgaagcgt gccggcttgg
    45781 attactggga caagttagaa ctgcattttt atgatcattt agaacagttt atcaatcaat
    45841 gtcacggaca gcttcacctg attagcaagt ttgcagttaa taattattct caagctactt
    45901 atgctgatgg cgattcccat tatttcttgt ttggacgaga agatacaggc ctgccagaag
    45961 attttatgcg agaacatgct gaaaaagcac ttcgtattcc gatgaatgat gagcatgtac
    46021 ggagtttaaa tgtctcaaat acggtttgta tggtgattta tgaagccctt agacaacagg
    46081 gctttcaagg gttagagtta aagcatactt acgaacacga taaactaaaa taatggactt
    46141 tttaaaagcg tcagtttttg gcaacatgcg ttgtcagtag ctgacgcttt taaaaattta
    46201 gtcaaagcta ttgctatcta ggtttaaatc tgctatgcta gaagtgtctt cagggcaggg
    46261 tgtgattccc gaccggcggt gactaggtaa ctagaagtcc gcgagcgcaa gctgatgtgg
    46321 tgtaactcca caaccgacag tatagtctgg atgagagaag accgggcttt ttgtgtaggc
    46381 ttattttagc tacacaataa cgacggaact tctttcaatt tgaaaaaatt ggaggaattt
    46441 tttaatgtca aaaacacata aaatgattat gattggtata ctttctgcga tatcatttct
    46501 attgatgcta gttagctttg ccattattcc aggcgcagct tttttaaaaa ttgaatttag
    46561 tattattcct gtcttatttg gtttaatgat tatggactta aagagtgctt acttgatttt
    46621 gttattgagg tccttattga aactcttttt gaacaatcgt ggggttaatg attttattgg
    46681 tctcccaatg aatattatcg ctatcgcttt atttgtgacg gcttttgcct tagtttggaa
    46741 ccgtcaaaaa acgcttagtc aatatgtatt tgctagctta ctaggcacag ggttattaac
    46801 gtttggcatg gttgttctca attatacttt tgccattcct ttatatgcta tatttgcaaa
    46861 tattgatatc agagcttata ttggtgttac taagtatatg atgactatgg ttattccgtt
    46921 taatcttgtt gaggggttga tatttgcaat taccttttat tttgtgtata ttgcaagtaa
    46981 accaatttta gaaagatact tacactaata tgatgacaaa caaacaaact cactttttaa
    47041 tagcttcttt tgcattgcta atctttgtta taataggcta tacggtcaaa ttttttcctg
    47101 aacgtttggc acttctagat aatactattc aagcagagat tagaggcaac cttcctatag
    47161 tgctgacgca attctttagg ggtgttactg tatttggaaa tgtcatgaca caagttttgt
    47221 tagttattgt gtcggtccta gttttattct ttatgaagtg gaaaattgaa gccttgttta
    47281 tcttgtcaaa tggtgccatt gcagcatttt tgattacgac tctcaagctc ttttaccaaa
    47341 gaccaagacc tgctattgag catttggttt atgctggtgg ctattcgttt cccagtggcc
    47401 atgcgatggg tagcatgctg atttttggta gcctattgat tatttgttat cagaggctac
    47461 attcaaaact attacaattt gtgactagca tgatctttat aatactaatt ttattaattg
    47521 ggttatctcg tatttatcta ggagtacact acccaagtga tattttagca ggctttgtat
    47581 taggatttgg tattttgcac tttatttatc ctttttataa gcaaaaacga tttgaatggc
    47641 gatttctttt gaaacaagac taatttttct tgagaagaca taggcttatt taaatcattt
    47701 agaataacat tggcaagcat cacccccttg attgccagtg ttattttagt tttagttaga
    47761 tgatgtgagc tgatgattag taaatatgtt tagataaaaa acgattaaag tgctatgtaa
    47821 cgtcagaaaa gacgtgattt ttgttgatct gtaatcatta agtaagctga tgtagaagta
    47881 gtgaattcta atgactgagc tatcttttgc atagatgact atcttgctag taatgatttg
    47941 tgctacaata gaaacatgaa aaaaagatat cagacattaa atgagcatta ccgtcaacta
    48001 tttggcgcaa aaatgtttaa agttccgatt gatgccggct ttgattgtcc caatcgtgat
    48061 ggaacggtag ctcatggagg atgtactttt tgcacagtat caggttctgg agatgctatt
    48121 gtagcaccag atgctccaat taaagagcaa ttttataaag aaattgattt tatgcaccgt
    48181 aaatggcctg atgtcaatcg ctatttggtt tattttcaaa actttaccaa tactcatgat
    48241 acagttgatg tgattcgcga ccgttacgaa caagctatta acgaacctgg agttgttggt
    48301 attaatattg gcacacggcc tgactgtcta ccagatgata ccattgctta cttagcagaa
    48361 ttatcagaac gcatgcatgt gacagtggag ctaggcttac agacaactta tgaagaaact
    48421 tctaggctaa ttaacagagc acattcttat gatttgtata aagaaacggt gaggcgacta
    48481 aggcattacc ccaacattaa tattgtctcg cacttgatta atggattgcc aaaagaaact
    48541 catgacatga tgcttgaaaa tgtgagacgc tgtgtaacag acaatgacat tcaaggaatt
    48601 aaattgcatt tgctgcattt gatgaccaat actagaatgc aaagagatta tcatgaagga
    48661 cgcttaaagt tgttgagtca aaaggactat gtttcgatta tttgtgacca gctagagatt
    48721 atccctaagc atattgtgat tcaccgtatt acaggagatg ccccacgcga tatgttgatt
    48781 gggcccatgt ggagtctaaa taagtgggaa gtattgaatg ccattgataa agaaatggaa
    48841 agacgaggaa gctttcaggg ctgtaaagtt gactaatttg tctcattttg acggatttgg
    48901 ataggagaaa agtgatgcta aaacgcccca ttcatctctc gcatgatttt ttagcggagg
    48961 ttgtggataa gtctagtgtg gttgttgatg ccacgatggg taatggaaat gatactgcct
    49021 ttttggcaca attagctaaa aaggtttatg cttttgatgt gcaagaacaa gctattagaa
    49081 aaacaagtga gcggttagcg cagctaggat tatcgaacgc tgagctgatt ttagcgggtc
    49141 atgaagcggt tgatcagtat gtgacagagc cggtgcgcgc agcgattttt aaccttggtt
    49201 atttgcccag cgcagataaa tctattatta ccttaccaaa taccacactc caagctttat
    49261 caaaactact aacgttatta atggtaggtg ggcgtattgc tattatggtc tattatgggc
    49321 atgatggagg cagtttggaa aaagatgctt tattagattt tgtcaagcaa ttagaccaaa
    49381 gaaaggtttc tgcaatgctt tatcagcctc ttaatcaggt gaatacccca ccttttttaa
    49441 tcatgttgga gaaattagca gacgtttagc tcttatccct gtgtcaagtc tactagaagt
    49501 tctgcgatta tttcggtgga aaaagaagaa atcccatagg ctttggctat aaatgtgata
    49561 taatgaagtc actatattat ttttttagga gttacattaa catggaagac cctgtgagtc
    49621 agcccttagt gattcaattt ttattgttag ttgttttaac cttgttaaat gctttttttt
    49681 cagccagtga aatggcctta gtttctctca atcgttctcg ggtggaacaa aaagcagcag
    49741 acggtgataa aaaatacgct cgtttgttgc gggttttaga ggaacctaat cattttttat
    49801 caacgattca agttgggatt acctttatta gtttactatc aggagcaagt ttatcagctt
    49861 ctttgggtaa ggtgatctca ggttggctag gtaattcagc gaccgcaagg acagctggta
    49921 ctatcatctc cttggttttc ttgacttatg tctctattgt tttaggagaa ttgtatccaa
    49981 aacggattgc catgaacctc aaagacaagt tggcgattgt ttcagcccct attatcattg
    50041 gattagggag actggttagt ccctttgtat ggctcttatc agcttctact aatttactga
    50101 gccgacttac ccctatgacc tttgatgatg cagatgagca aatgacacgt gatgaaatcg
    50161 agtatatgtt atcaaaaagt gaggcgaccc ttgatgctga agaaattgag atgttgcaag
    50221 gagttttctc acttgatgaa atgatggcgc gtgaagtcat ggtcccaagg accgatgctt
    50281 tcatgattga cattaacgat gatccgcttg aaaatattca ggaaatctta aaacaaagtt
    50341 tttcacgcat tcctgtttat gatgtggata aagataaaat tatcggtctc atccacacta
    50401 agcgtctctt ggagtcaggt ttccgccagg gatttgatca gattaacatg cgaaaaatgt
    50461 tacaagaacc tctttttgtt cccgaaacca tttttgtaga tgatctctta cgccagctgc
    50521 gcaataccca aaatcagatg gctattttgc tagatgaata tggtggtgtg gcaggacttg
    50581 tgactttgga agacttgctt gaagaaatcg tcggtgaaat cgatgatgaa accgataaag
    50641 cagaacaatt tgttcatgag attggagaca atacctatat tgttgttggt actatgactt
    50701 taaatgagtt taatgactat tttgataccg aactagaatc agatgatgta gataccattg
    50761 ctggttttta tttgacaggt atcggaacca ttccaagcca ggagcaaaaa gaagcctacg
    50821 aaatagataa caaagacaaa catttagttc taatcaacga taaagtcaaa gatggccgta
    50881 ttacgaaatt aaaattaatc ctgtctaata tagaacagat tattgaggaa gactaggctc
    50941 catgcctagt ctttttgatg aaaagtgtta taatgaaaat gtaaaaacgt ttacaagtaa
    51001 tgaaggagat gcaatgactg agaaagatta tggacaagta acagggatgg tgcattcgac
    51061 agaaagtttt ggttctgtag atggtccagg cattcgtttt atcattttct tgcaaggctg
    51121 taaattacgt tgccaatatt gccataatcc ggatacttgg gagatggaaa ccaacaattc
    51181 aaaaatcaga acagtcaatg atgtcttgaa agaagccctt cagtacaaac atttctgggg
    51241 aaaaaaagga ggaattaccg tatctggtgg tgaagctatg cttcaaattg attttatcac
    51301 tgccttattc atcgaagcta aaaaattagg tatccatacc acactagaca cctgtggctt
    51361 tacctataga ccgacacctg aataccatca ggttcttgat aacttattgg cagtgaccga
    51421 tttgatttta ttggacttaa aagaaattga tgaaaaacaa cataaaattg tcacgcgtca
    51481 gcctaataaa aacatattgc aatttgctag gtatttatcc gacaagcaga ttcctgtctg
    51541 gattcgacat gtcttggttc caggcttgac tgatattgat gatcatttga cacgattggg
    51601 tgagtttgtc aaaaccttga aaaatgtcga taaatttgaa gttttgcctt accataccat
    51661 gggagaattc aagtggcgtg agctaggtat tccttatcag ttagaaggtg ttaaaccacc
    51721 aactaaggaa cgcgttcaaa atgctaaaaa tctgatgcag accgaatctt acacagaata
    51781 tatgaatcgg attcatcagt cataagagct gttatagggt tgaggtttcc tcaacttttt
    51841 aaagtattat aagcagatct tttgtaatct tcctttgatt ttagggggta attttgctaa
    51901 aataaaatga ataaacaaaa tgaggtagaa tgattatgtc aaaaatttta gtttttggtc
    51961 atcaaaatcc tgatacggat gctattgcat catcttatgc ttttgattac ttatcccaaa
    52021 aagcgtttgg tttggatact gaagtcgtag ctttaggaac acctaacgaa gaaacagctt
    52081 ttgcccttga ttattttggt gttgaggcac cacgtgtggt agagtctgct aaagcacaag
    52141 ggtctgaaca agtgattttg acagaccaca atgaattcca acaatccatt gcagatatcc
    52201 gtgaggttga ggtatatggt gtggttgatc accaccgtgt ggctaatttt gaaactgcaa
    52261 atcctttata catgcgtgtg gaaccagtag gatcagcctc atctattgtt taccgtatgt
    52321 ttaaagaaaa tggcatcgag gtgccaaaag cgattgcagg gatgctcttg tctggtttga
    52381 tttcagatac cttgttgctg aaatctccaa caactcatgt gtctgatcac cttgtcgctg
    52441 aagaattggc agagctagca gaagtgaact tggaagacta tggaatggct cttttaaaag
    52501 caggtacaaa ccttgcaagc aaatcagaag tagaattgat tggtattgat gccaaaacat
    52561 ttgaattaaa tggcaatgct gtgcgcgtgg cacaagtgaa cactgttgac attgcagaag
    52621 tcttagaacg tcaagaagct attgaagcag ccattaaaga tgccatggct gcagaaggtt
    52681 attcagattt tgttttgatg atcacggata ttgttaactc aaactctgaa attctagcta
    52741 ttggagcaaa catggacaag gttgaagcag cctttaactt cacccttgac aacaatcatg
    52801 ccttcttagc aggtgccgtt tctcgtaaaa aacaagtggt tccacaattg acagaaagtt
    52861 ttggagctta atgaaaagac gctttaaaag gacttgattt ttttgaccaa gtccttttgt
    52921 cactcttcta tgttataata gaaggtatga taacagtatt tcattcagat aaactcacca
    52981 gacaaccttt ctttcaagac cttatcaatt atctcgacca gcatgatcac gttatattac
    53041 gagagatcaa aaaagccttt cctaatgtga caggtattga caaggccatc gaaagctatg
    53101 ttcaagctgg ctatattcgc cgtgaaaata agcgttatgg aatcaatctt cctttggtga
    53161 gttctgatca gcagctggcc ttagacacta tgctttttgt ggacacctgt tcagctatgt
    53221 atgaaaatat tttagcggtt gtttttgaga ctcagctaac aaaccaaacc aatcacgtga
    53281 tgatcaagga aaagaccaac atcacgagag acgatttgac cctggctaat tatttttacc
    53341 gtctcaaaag aggtgagaag ccatcagctg agcagctgga cttgtatgac ctcttggggg
    53401 atgtgaatca ggaatatgcc cttaaatata tgacaacttt tttgcttaaa ttcatgcgca
    53461 aagaccttgt gatgcaaaaa cgtcctgata tatttgtgga agctctggtt acacttggtt
    53521 acctaaaaca agtggagcct acaacatacc agctgctaat gaccttggat aaagaaagtt
    53581 taacctttat agcaccatag aaaaaagcta ggaacaagga ctgttcttag ctttttgagt
    53641 gcttttattt tgtttttgcc attaagtaaa gaaaataagg ggctccgata agcgacacta
    53701 aaatccctgt ctggatatta gtgccaacca gatacactcg cccgactgta tctgcaacaa
    53761 gaagtaggat cattccgatt aaaatactaa tgggtattgt tattttatga ttactcccta
    53821 ccaagtatgt actaaagtgt ccagctatta agccgataaa actaatatta ccaaccaaaa
    53881 gaacactcaa agcacctaag ccagctgcta agacaagtat caaacgacgc ttacggttta
    53941 aagggagtcc tagcccaata gcagtattat cagctaatcc cataatatta aggaaatggg
    54001 cttgactata agtcaatagc caaaaacaca ataataaagg agcgatgaca ctcagagtag
    54061 gccagtcgtc tcctattaat tggccgctaa gccaattgat gaccaaatcg actttgtagc
    54121 gattaatatg accaacaaga gcaaccatta gacttgaaag catggtagta acagctacac
    54181 cagtaagaat taaccgtgta ggatcaattt gtccttgttt tgtcaaagat agccaataaa
    54241 cgctaaaggt tgttacaaca gcaccaagca tggcaaataa aggcaagtaa tggctgatat
    54301 gagcttgctc aaagaaagaa aaggagacca caatagccaa agagatacct gctcctatag
    54361 taatacctaa tacaccagag tcagctaagg gatttcgtgt taaacgttgt aataatagcc
    54421 ctgataaagc aagagaaccg cctcctagac aagcagctag aattcttggc agacgaatgt
    54481 taataacgat gaaagatatg gcatggctag attttcccaa aaacacatgg ataaggtcca
    54541 aaaaagatag atgagattct ccaagggaca aagctaatcc cataagactt acaagtagca
    54601 ggatcaggct agtatataac cccaaaaggt gtcggtgaga tttcaatagc ggcctcctct
    54661 tcttattaac cataaaaagc atggaaaacc aattaagcta accaatgccc caagaggggt
    54721 ttcataaggc ggattgagat tacgacaagc aatatcaacc cataccatga aactagcgcc
    54781 agaaactgca caaagcggca aaaggtatcg gtaatgatga ggtgtaaagt gtttcataag
    54841 atgaggaatg actaagccga taaaagaaat gcttccagca atggctacag ctgctgatga
    54901 taaaataaga accaaaatca taaagactgc actgatcaag ttggtctttt gcccaagagc
    54961 ttttgcctga gactcactta gactaaggac ggtcaggtga taagataata gctgggctaa
    55021 acaaagacta agaataataa gaggagcaat atagccgatc atttgccaat tgaccccgac
    55081 aagacctcct gcttgccagc cgataacagc atttgccaga tgatagtaat tggtaatacc
    55141 ttgacctaat gctgatagca gtatggaaac catagctcct gctaagacaa ggcggagctg
    55201 atggtagcct ttgccagatt gataagaaag gccaaagact aaggttgctg ccaggctaga
    55261 ccctaacaac gaaagcagaa taatgaggga atagtgcaag tgaggtacaa acgcataagc
    55321 cagtaccaag gctagtcctg ctccagcatt aatgcctaaa aggccaggct cagcgatagg
    55381 attacgtgtg attgcttgca tgatagttcc agaaacagct aacgcagagc cagttaaggt
    55441 agcaccaaaa agtctcggta atcgtatagc gagtaaaaca ttggctttat ggccgttttg
    55501 ctttccaaat aaaatagagt taagatcctg atgtgataaa gcaacggctc caaagcgcag
    55561 atgacagtaa atagcaacac ataagctaaa actaagtagc acaaagacaa gccaaaagat
    55621 atgtgactta gtgattatct gcgttttttt taagcaaaaa cgctcgagac acaaaaaggg
    55681 atgcttagtt ttcacttgat aagattgcct ctcgtaaagt ctctaattga tattctaaag
    55741 aaagaggatc agtaaagtaa aaaacattgg cattgacttt gatcacatgg tgttttttca
    55801 cagctggaat ggattgccat agcttacttt catatagagc agaaccagtc ttatcatctt
    55861 ctgcagcaac gacgacataa tcaccaatat agtctggcaa gacttcctgc gacaatgaca
    55921 ggtaaccttg tttaaaaact tcagtcttca ctttttcagg agcatcataa tggaaagctt
    55981 gatggataac ctctccgcca cgtccccagt ctttaccaaa aaggtaaacg tctttttcat
    56041 aaagccccat aatagtaaag gttgctttgt cacctgtaac ggctttgact tctttttcat
    56101 aagctgctgt tttagttttc caatctttta accatttctt ggctttgcct tctttgttaa
    56161 agatgcggcc gaagtcagat aacacttgta aataatcacg tttgcggtat tcaatcgaaa
    56221 taacaggcgc aatttctgcc aattgtttaa tattttcttc tgtagagcca acaacaatga
    56281 ggtcaggctt aagcgttgta atggcttcta aatcagttgc agcaacttgc tttgcttttt
    56341 ttacagtctt tgctaagatg gggtttttct tgtcataaga agtaaccccg actagattca
    56401 tatctaattt tttgaggtaa ccagtgtaag ttgaagccag actgactact cgcttaggct
    56461 gtttaggaat atctccgtaa taagtcacac cagcaatttg aggcataggc gataaacttt
    56521 ttgaggcggt gttagaatga ttagttgctt gattaccaca ggcgattaag gtaatagttg
    56581 tcaaacagag tgttaatagt agagttaatt ttttcatgta tgctaattct cctagaataa
    56641 agttattgaa gctgataagt cagcactata ggacagttgt ggataggatc atcaaccagt
    56701 accggtttga tttggaagat gtcttgaatg ataggtgaag tcataacatc tgctatagta
    56761 ccagtataat ggatcttgcc atgtttcata gcaatcagat gatccgaata gcgggctgaa
    56821 agattgaggt cgtgaagaac cataacaata gtcttgccgg catccttatt tagggattta
    56881 acgagctcta ggatttctag ttgatgattt aaatctaaat aagtagttgg ctcatctaaa
    56941 aaaatagtat cagtgccttg ggctaatgcc attgcaagcc aaaccctttg acgttgcccc
    57001 ccagaaagag catcaacagg ttgatcagca taggccatga cattggtggc ttgcatagcc
    57061 caatgaatgg cttttttgtc agctggacta agatttccaa agtaagattg atggggaaaa
    57121 cgtccgtaag aaaccaattc gtaaacagtt atgccgttag ttgcttcttg aacttgaggt
    57181 aaaagagcta gttttttagc aacctcttta gtttctaatg tggcaatgtt ttgcccattt
    57241 aaatatacaa ctccctgttt tggtggtaat aatcttgtca gtgcttttaa taaactagac
    57301 ttcccacagc cattggcgcc aataatggtc gtaattttac cttcaggaat ataaaacgat
    57361 aatttatcga tgatggtacg ctgttcatag gcaattgtga ggtcttcagc actaattgtt
    57421 gtcatatggt tgtctccttt ctaaaaagag tcaaagggtt gatgaaagtg cacattaaag
    57481 tattcatctg cccattttaa aatagttgag aagtagctta ttttctcaaa atcttcacta
    57541 ttatagcata gaaaaaaggc ttaacaaagc cttttttgat gtgattttta ggatgtatta
    57601 aaaaagaaat attagtttag aatgattaaa aacaatagcg ataatcattc taaactaacg
    57661 tcaacatact ctagaggtag tgtctggcga ttgctaaatc accagcataa gctacttgtt
    57721 gtcctttaac aatttgataa gcatctgcgc ctttcccagc gataatcaca gcatcttttg
    57781 ctccttggca aagagacatg gctttttgaa tagcttgctc acggtcacta ataatctcta
    57841 ctgggcgtgc gatatggctg gcaatttctt ttgaaatatc ttctggatcc tcgaaattag
    57901 ggtcatcagc agtgagaatg acggtgaggt ttggatgctg atgaatcacc ctaccaaaat
    57961 cagctcgtct gctctcgcct ttatttcctg gtgcacctaa aatcaacatt aatttgcctg
    58021 tctgatgttc ttccacaacg ctgagtaatt tttctaagct gtcaccatta tgggcataat
    58081 caacaaaaac tttagcatga tttgtcatag tgaggacttc catacggcct gggacgcgag
    58141 tcttagcgat acctttttga atatcagcta ggctagcacc caaacgaagg caggcaagtc
    58201 cagctgccat agcattttct tggttaaaat ggccaattaa ttgaatgtca taatggccag
    58261 ctaattgtcc tttagcttca aaggagaagg cttggctagt ggtgatctgg ttgtcagaca
    58321 agggaccata aaacacatgc tcttgatccg ctacctgatc cgctagaaat gaaaaatgat
    58381 ccatcccgct attgatcaca acagctctgc tattctccat taaaagacgc ttatgataga
    58441 aataatcttc aaacgttgga tgctcgattg ggccaatatg atcaggacta atatttagga
    58501 aaaccccaac atcgaacgta agcccataga cacggtcaac gaggtaagcc tgactagaaa
    58561 cttccataat caagtgggtc atgccattag tcacacactc agccatcata gcaaaaagat
    58621 ccaagctctc aggagttgtg agttgtgatt tgaaaaaggt cttgccatct aaggtggtat
    58681 tcatggtaga aaacatggca ggtttatagg attctttcaa catgtggtag gcaaaataag
    58741 cagccgtcgt ctttccctta gtacctgtaa aagctagtag tttgagcttt tcttgagggt
    58801 tgccatagaa aaccatggca attagactca tagctttttt gatatcagtg accaaaacaa
    58861 cgggaatacc aagctcgtaa tctacctctg aaatatagag ttgtaatccg ttagtgattg
    58921 cttctttaag gtagtcagct ttgaaagttg cccctttagc aaaaaaaagg gtcttgccat
    58981 ctacttgacg gctatcgtaa ctcaagcgct caaaactaaa gccttgataa tggtagtggt
    59041 atccgtcggc atcaagaacc tcacgaaaat tatggtcttt ttttaaaatg tctaataatt
    59101 gttcaatggt tatcataccc ttattttatc cttaaagttc ttgttttaca agtcacaagc
    59161 aaaagtttat aataaagcta atagaaacta ggaaagaata gatatgtcga cagaaaaaaa
    59221 acaactgaca caagaagaat tgatggttca gggagcagcc tggtccacag ctggcaattt
    59281 cattagccga cttttgggag tgttatacat tattccctgg tatatttgga tggggcaata
    59341 cgcgattcaa gccaacgctc ttttcaatat ggggtataat gtttatgctt attttttatt
    59401 gatttcaacc acaggactca atgttgcgat tgctaagcag gttgccaagt acaattccat
    59461 gggacaaact gagcatagct atcaattaat ccgaagtacc ctaaaactca tgcttggtct
    59521 gggccttatt ttttcagcca ttatgtatct gggctctcct ttatttgcga gcttatctgg
    59581 tggtgatgac acacttgttc ccattatgca cagcctttct ttagcagttt ttatttttcc
    59641 tgtaatgagt gtcattcgag gtatctttca aggccacaat aatatcaagc cttatgcgat
    59701 tagtcagatt gctgagcaac tcattcgggt tatctggatg cttttgacca ccttccttat
    59761 catgaaatta ggttctggtg attacgcttc agcagtaact cagtcaactt ttgcagcctt
    59821 tatcgggatg gtggctagta tgggtgttct ggggtactat ctttggaaac agggacttct
    59881 agcagctatt tttagtaagc cagatcatac tgtttccatt gatatcaagg gcttgttgct
    59941 tgaaacctta aaagaatcca tcccctttat cgtaacagga agtgccattc aagcctttca
    60001 attgattgat caatggactt ttgtcaatac tatgactctt tttacagatt atagccgttc
    60061 tcaattgctt gttttatttg gttactttaa cgcaaaccca gccaaaatta caatggtttt
    60121 aattgcagta gcagcatcta ttggtggtgt aggcattgcc ttattaactg aaaattatgt
    60181 caaaaaagac atgaaagcag ctgctcgctt aatcattaac aacattgaaa tgttagtaat
    60241 gtttttgtta cccgctctta ctggggcaat tattttagca agacctctat attctgtttt
    60301 ttacggagct agcgaggatc gtgccattca cctctttgtg gcggtcctct tccaaacctt
    60361 gttgatggcg ctttacaccc ttttttcacc gatgcttcaa gctctttttg aaaatcgaaa
    60421 agcgatttac tactttgcct atggtatctt gattaagtta gttttacaga taccgcttat
    60481 ttatttgcta catgcttatg gtcctttact agcgacgacg attgctttag tggtgccgat
    60541 ttatttgatg tatcgacgcc tatatcaggt tactcatttt aaccgcaaac tgttgcaaaa
    60601 acgtttatta ttaaccttga ttgaaacctt attaatggga ctggtcgtgt ttgtggccaa
    60661 ctggctattg ggctatgtct ttaaaccgac aggccgcttg accagccttc tttacctcct
    60721 cattattggt ggcttgggaa tgacggttta cacggcactc accttgctga cgcatcaact
    60781 ggataaatta attggtagca aagctagtcg ccttcgtcag aaattaggct ggcattaata
    60841 taatgacacc aagaacgatt tcgtgagtgc ttatcgaaat cgtttttttc ttaatgatct
    60901 cgcttagtaa aggtagccga taaaaaatca ggatttccca agcaaactac tgatattttt
    60961 ggtataatga taaaagtgta aaaaaataga aggaagtata tcaacatggg aaaatgtcaa
    61021 gttatttcgc atccgcttat ccaacacaaa ctttcaatct tacgtcgtca aaccacatca
    61081 actaaagatt ttcgtgagtt ggttaatgaa attgcgatgc tgatgggata tgaagtgtca
    61141 cgcgaccttc ccttagaaga tgtcgacatc caaacgccag tatcaaaaac ggtccaaaaa
    61201 cagttagctg gtaaaaaatt agctattgtt ccgattcttc gtgctggtat tgggatggtt
    61261 gatggcttgt taagtttggt accagctgca aaagttggcc atatcggcat gtaccgtaat
    61321 gaagaaactc ttgaacctgt tgaatacctc gttaaactac cagaagatat taaccaacga
    61381 caaattttcc ttgttgatcc aatgttggca acaggtggtt cggctatctt agcagtggat
    61441 tcgttgaaaa aacgtggtgc agcaaatatt aagtttgtct gtttagttgc agcaccagaa
    61501 ggggtgaaaa aacttcaaga agctcatcca gatattgata tcttcacagc agcacttgat
    61561 gatcatttaa atgaacatgg ctatatcgtg ccaggattgg gagatgctgg tgatcgttta
    61621 tttgggacaa aataaaagag acaaccatca aatagtagcc tttttaggcc aatcgttctt
    61681 gattttgtta aaaaaatcag agagatttct cttctttaaa ggaaaagcaa gtttatgatt
    61741 tgacctttat tgaccaaaat attataataa agacaatcag taaaaaagag atgacctagt
    61801 gggttgccgt ctctcaaaat gacgctacta aaaacccttg tagattggag aatgtattat
    61861 gattcctgtt gttattgaac aaactagccg tggagaacgt tcttatgaca tttattcacg
    61921 tttattaaaa gaccgtatta tcatgttaac agggcctgtc gaagacaaca tggccaactc
    61981 tgtcatagcg cagctcttat tccttgacgc gcaagacaac acgaaagata tctacctata
    62041 tgtcaacaca ccaggtggct ctgtctcagc aggcttggct attgtcgata ccatgaactt
    62101 tatcaaggcc gatgtgcaaa ctattgtcat gggtatggca gcttctatgg ggacagtcat
    62161 tgcttcatca ggaacaaaag gcaaacgctt tatgttacca aatgcagagt atatgatcca
    62221 ccagccaatg ggtggcacag gcggtggcac gcaacaaacg gatatggcta tcgcagcaga
    62281 acatctctta aaaacacgtc accgtttaga aaaaatctta gcacaaaatg ctgggaaaac
    62341 gattaaacaa attcataaag atgctgagcg tgattattgg atgagtgctg aagaaacctt
    62401 gacttacggt ttcattgatg aaatcatgga aaacaacgaa ctaaaataaa gaggatatct
    62461 gcaaaagtat tctagaaatt tctaggatgc tttttctctt agaaaggaaa ataatgatac
    62521 agcctactgc attaaaacag cttcaagagt taaaacgtca gttcagatcc ctttgcaacc
    62581 aattatgaga tagtttgagg atgatcctta gcagctgtgt gcaagaagtt atgacgattt
    62641 cataaaggat gacctagcca gtctttcttc gtctctttta cttaaagtgt ggtataatca
    62701 gattatgcta gccaaaatta aaaaaggcat tgccattaaa ggtgttgtag atcggcttat
    62761 cctagatctc ataacaatgc tgtagtaccg cccttacctt gctactaaag gcgctgttga
    62821 agcacaaatt gacttgcaaa ggatcttgac tgataggtat atgtctggtg gaatcgccaa
    62881 agcagcaaaa agaccaagac ccttatttgg tctctaccaa aatataatag aaaaggattt
    62941 agaatgtttc aaaaacaaga acgtattgga ctcgtcgttt acctgtatta taaccgagat
    63001 gccagaaagt tatcaaaatt tggtgattta tattaccatt ccaagcgctc tcgttatctc
    63061 attgtttata ttaataaaaa tgatttagac acaaaattag aagaaatgag acgtttgaaa
    63121 tgtgttaaag acattagacc atcagctttt gatgatattg accgccaatt tgtaggtaac
    63181 cttcatcgcg acgaaacaaa caaccatcaa aagggttaac aaccccctag ttactgatta
    63241 gcgttttaga tttagttagc ttattaaagt aagaaggttt tgacagatgc tcacactact
    63301 tcaagacaat gagacaacta gagcattagt gaataactga gttgacggtc acttctttaa
    63361 taagaacgga taggattggt cctaaaatag agcactgcaa agatatgcca gtgcttttta
    63421 gctcattttt tgataaaata gaactactaa aaagtgaagg aaataagatg atcacaggaa
    63481 aactcataac cgttgaaggt ccagatggcg cagggaaaac gactgtttta gagcaactca
    63541 ttccactgtt aaaacaaaaa gtggctcagg agatcttgac aacaagagag cctggaggtg
    63601 ttgccatttc agaatacatt agagaactta ttttagacat caaccatact actatggacc
    63661 ctaaaacaga actcttactt tatattgcgg ctcgaaggca gcatttggtg gaaaaggtac
    63721 taccagctct tgaagcaggc cagttggttt ttattgatcg tttcatcgat agttcagtag
    63781 cttatcaagg tgcaggacgc ggtttgatca aagcagacat tcagtggctc aacgagttcg
    63841 caacagatgg tttagagcct gacttgaccc tttattttga tgttccttct gaaattggtt
    63901 tagcacggat taacgcgaac caacaaagag aagtcaatcg tttagatttg gaaacaatag
    63961 agatacatca acgagtacga aaaggatacc tagccttagc taaggagcat ccaaaacgta
    64021 ttgtcacaat tgatgcgact aaaccattga aagaagttgt ctctgtcgct ttggaacatg
    64081 tcttagcctt gcttttagcc taggataagg gaggggagga atggatttag cgcaaaaagc
    64141 tcctaacgtt tatcaagctt ttcagacgat tttaaagaaa gaccgtctga atcatgctta
    64201 tcttttttcg ggtgattttg ctaatgaaga aatggctctt tttttagcta aggtcatctt
    64261 ttgtgagcag aaaaaggatc agacgccctg cgggcattgt cgctcttgtc aattgattga
    64321 acaaggagat tttgccgatg tgacggtatt ggaaccaaca gggcaagtga ttaaaacgga
    64381 tgtggtcaaa gaaatgatgg ccaacttttc tcagacagga tatgaaaaca aacgacaagt
    64441 ttttattatc aaagattgtg acaaaatgca tgtcaatgcc gctaatagct tgctaaagta
    64501 tattgaggag cctcagggag aagcttacat atttttattg accaatgatg ataacaaagt
    64561 gcttccgacc attaaaagtc ggacacaggt ttttcagttt cctaaaaacg aagcctatct
    64621 ttaccaattg gcacaagaaa agggattatt aaaccatcag gctaagctag tagccaaact
    64681 tgccacaaat accagtcatc tagaacgtct gttgcaaacg agcaagcttt tagaactgat
    64741 aactcaagca gagcgttttg tatctatttg gctgaaagat cagttgcagg catatttagc
    64801 gttgaaccgt ctggtacagt tagcaactga aaaagaagaa caagatttag ttttgaccct
    64861 tttgaccttg ctcttggcaa gagagcgtgc gcaaacgcct ttgacacaat tggaggctgt
    64921 ctatcaggct aggctcatgt ggcagagcaa tgttaatttt caaaacacat tagaatatat
    64981 ggtgatgtca gaataagtgc aattggaatt cgttatgggg aaacgaagct tgtcaaaaga
    65041 cttgcccgta ctcccttttc agcagaagag cacatggatt tttacaactg ttaaaagatt
    65101 tagcggccga ttttaggtca agaagtgagc tgccccagat taacagccaa gaagatttca
    65161 ttttctatgg aggctatgga ccgtgtggca ggccactgtg ctgcattcgt ttttgggaaa
    65221 atttctgaca gtatctatta aaatactaaa aaatcaaggg ttatcattga ctttgggtaa
    65281 agcaacaagc ccttatggcc gtttgctttg ctgtctctat gatgaaggtg gcttttacca
    65341 ataaagcaag aaaaaattcc ctgaatatgg taccatagta gagacaagtg atggccctgc
    65401 aacagtagca gccgttgata tatttacaga tcccattaag gtgcggttaa aagaccagtt
    65461 gaccttagtc acttatgctt tagaggaggt taaagtaggt gaataaaaaa gaattattcg
    65521 atgcttttga tggattttcc caaaatttaa tggttacttt agctgaaatt gaagccatga
    65581 aaaaacaagt ccaatcacta gtagaagaaa acacgattct tcgcctagaa aatacaaaac
    65641 tacgggaacg tttgagtcac ctagaacatg aaacggtagc taaaaatcct tccaaacaaa
    65701 ggaaggatca cttagaggga atttatgatg aagggtttca tatctgcaat tttttctatg
    65761 gacaacgtcg tgaaaatgat gaagaatgta tgttttgcag agaattatta gatagaaagt
    65821 aagggcatgc aggttcaaaa aagttttaaa gacaaaaaaa caagcggaac cttgtatttg
    65881 gttccaaccc caattggcaa tctgcaggac atgaccttta gagcagtggc aacgttaaaa
    65941 gaggttgatt ttatttgtgc agaagacaca agaaacacag gtcttttgct aaaacacttt
    66001 gacattgcta ccaagcaaat cagctttcat gagcacaacg cctatgagaa gattcctgat
    66061 ttaattgatt tattaatatc aggacggtca ctagctcagg tgtctgatgc aggaatgcca
    66121 tcgatttctg atcctgggca tgatttggtc aaggctgcta ttgatagtga tatcgcagtc
    66181 gttgctttac caggtgcctc agctgggatt acagctttga ttgctagtgg actggctcct
    66241 caaccccatg ttttttatgg gtttttaccc agaaaagcag ggcaacaaaa ggctttcttt
    66301 gaggacaaac accattaccc agaaacacaa atgttttatg aatcgcccta tcgcatcaaa
    66361 gataccctga ccaatatgtt ggcttgttat ggtgatcgtc aagtggtgct tgtcagagaa
    66421 ttaaccaagc tttttgagga gtaccaacgg gggtcgattt cagaaatctt gtcctacctt
    66481 gaggaaacac ccctcaaagg ggagtgtctg ctgattgtgg ctggagccca agtagacagt
    66541 gaggttgagc tgacagctga tgttgactta gtgtctctag tgcaaaaaga aattcaagca
    66601 ggagccaagc caaatcaggc tattaaaaca attgctaaag cttaccaggt aaatcgccaa
    66661 gagctttacc aacaatttca tgacttataa gtcatcgtag gttaggagaa aaacaaatga
    66721 cagaagatca agcaactatc cttattttag gagtatacgg aatcatattg gctgtattct
    66781 tattatcagc tttattatgc cttatcgcta actggcgact ctttaaaaga gcaggttaca
    66841 aaggatggta tagtctcatt cctttctatg caggttttgt caaacacaaa atcacttttg
    66901 gagaagataa taaatggttt tactttattg gttgggtcat tgctatttat tattactatg
    66961 tgcacttttg ctatgtccgt gcttttggtg gatcaaaggg tctagcagta tttggcttgt
    67021 ttttcccagg cattgcttct ttgatagtgg cttttggaaa aacttatacc acacaagaat
    67081 gccaccctgt gccacatctt ttaaactaag agaaaaaaga ccatcaattg gatttattga
    67141 tggtctttta ggtgtttaaa tgattttagt cccgtgaaca atggccgttc cagtgagttg
    67201 actcaagctt tggcaattct cagcagtgac gcctccccca atcatgattt caatgcgttt
    67261 attggcatag gtcacaagac ttttaagctg ttccacattg tctgtaatag gagtagcttc
    67321 aggtgagcca tgagttagca cacgcacaaa gccataatca atcagttggt caagcgcttg
    67381 gtgctggtga tcagtcggaa tccggtcaaa agccatgtgg aaaaccagtg gcaagccttg
    67441 tgtagcaggc agtaattgct cgatagcgtc ggtatccagt tgattttctg aggtcagcaa
    67501 gcctaagact agcgcatctg agccagcttc aacagctttt aagatatctt cttccatagc
    67561 ttttagttcg aggtcgttat agacaaaatc gccaccgcga ggtcgaatca tggtcgctac
    67621 gctaatcttt ttatcgtgta agagctgaca ggcttctttg atgacgccat aagatggcgt
    67681 ggtccctccg acagctaggt tatcacagag ttccacacga ctgacttgac cagcatcaag
    67741 ggttggcagt agtgtgaggt tctcagcaca aaattcttta atcattttta gttctccttt
    67801 atcactaata ttatagcacg aatgaagcta gtcatcagga aacatttttg tcaaatcatg
    67861 gacattatgc tatactcact agtattaaaa gtaggccaaa acagcagcaa atactttgaa
    67921 aaaagattgc tatgttctac ggcaatatca gtctttacca acgaactctc aagaagaaaa
    67981 gaaagacaaa gcttagcatt ttaaaaaagc cccccttttt ttgtgaaatg atgaccaaaa
    68041 acaggcgcct cacgcctaaa acagttaccc taaaggagaa caaatgatta cgatttacca
    68101 atacccaaaa tgtagcacct gccaaagagc catggctgaa ttaaagcaac tggttagtga
    68161 ctttgaagcc attgacatca aagctaatcc acccaaagct caggacctca aacattggat
    68221 ggaaacatct ggttatacca tcaaaaattt ctttaatacc agtggtaaca gctaccgcga
    68281 attgggactt aaagataaaa tagaccagct tagccttgat aaagctgctg aacttttagc
    68341 cacagatggt atgttgatta aacggcctat tctgattaag gacggtaacg tcttacaagt
    68401 tggctatcgc aagccatatc aagagttgga tttaggataa tgcgacaaag tctcaaaaaa
    68461 ggacaaggca acattataac gccttgtcct tttttgggat ctgtttttag acattagaga
    68521 tcaatatcta gtaaaattgg cgtatggtct tggcgttccc cagaagaaat catttcagaa
    68581 cgcttaacct tatccacgat tcggtttgaa gctagccaat agtcaattct ccaaccggta
    68641 ttattgattt tgctggtttt actacgttgt gcccaccagg tataaacatg aggaatatcg
    68701 ccgtgcaagt gacgaaaagt atctgtaaag cctcttgcta ataggttagt aaagccttga
    68761 cgttcttcgt cggtaaaacc aggagaacgt cgattgctgc ttggatttgc caaatcaatc
    68821 tctttatggg caacgttgta atctcccgta gccaaaacag gcttttgagc atccagttca
    68881 gtcaaataat cagcgtattt gtggtcccaa atttgacggt catctaaccg tcttagacca
    68941 tcaccagcat taggcgtata aacctgtgtg acgaaaaagt cttcaaattc tagggtaatg
    69001 atacggccct cagcatccat tgttgttggg gcaccaattt ctggaaaagt aatgacaggg
    69061 tttaacgtgt ttttgtacag gaacatggtg ccagcatagc ctttcctagc aggctcaact
    69121 gaagaacgcc acacatgcaa atagtttgga aagtaactaa gtagggtctc aatatgtttt
    69181 ttggtaggcc ccttagcaga taatttagtt tcttggatgg caataatatc cgcatcttga
    69241 gcgactaaag tgtctaaaac agcgcgtgat aaaagcgcac gaggggactc tcctgtgaga
    69301 gcagcattta aggaatcaat gttccatgaa atcagtttca tttggtttct ctttctaaca
    69361 gatagtcttg ttgttattgt aacaaaaaac gaaacgataa gcgactttgg gcacctagcc
    69421 cccataaaac agtctagcca aatgaagcat agcagcgtaa gaacaagaca aggaggtgat
    69481 cagtgagcat atcgtttgac agttaagagt aggattacta taatagaaac ataaatgtaa
    69541 gcgctaacaa aataaaaaat ataggagaat gtcaatggca caaaaaacag tcatcaccga
    69601 agaaacaacc gattttgtta tggatttcaa aacaagtagc gctgagggaa atgttgattt
    69661 tattaacgtt tttgaccttg agaaaatggc acaacaggtc atccctaaag gagcctttgg
    69721 ttatattgct agtggtgctg gtgatacctt taccctccat gaaaatattc gctcatttaa
    69781 tcataaatta attgtccctc actccctcaa aggggttgaa aatccgtcaa cagagattac
    69841 gtttgatggt gatcacttaa cctctcctct tattttagca cctgtagcgg ctcataagct
    69901 ggccaatgaa caaggagaag tagcgtctgc caaaggattg aaggaatttg gaagtatcta
    69961 caccactagt tcctactcaa caactgacct gccagaaatc agtgctgcct taggtgggac
    70021 accccattgg tttcaatttt attgcagtaa agacgatggc attaatcgta atattatgga
    70081 tcgtgtcaaa gcacaaggtt gcaaagctat cgttttgaca gcagatgcaa cagttggagg
    70141 aaaccgcgaa gttgataggc gaaacggctt tgtttttccg gtcggtatgc caattgttca
    70201 agaatattta cctgatggtg caggtaaaac aatggattat gtctataaat cagctaaaca
    70261 agcgcttacc tcaaaagata ttgaatatat cgcaacctac tcaggcctgc ctgtttatgt
    70321 caaaggacca cagtgtgctg aggatacctt gcgggcattg gacgcaggag catcaggtat
    70381 ctgggtaacc aatcatggtg gccgtcaatt agatggtggt ccagctgctt ttgattcctt
    70441 acaagaagtc gctgaagcag ttgatcacaa agttcctatt gtctttgact caggtataag
    70501 acggggtcaa catattttca aagctcttgc ttcaggtgct gaccttgttg ccttggggcg
    70561 tccagcgatt tacggcttgg ctatgggagg aagcattggt actcgtcagg tctttgaaaa
    70621 gttaaatgac gagttaaaaa tggttatgca actggctggg acacaaacta tccaagatgt
    70681 taaagccttt aaccttcgcc acaatccgta tgattcatcg attccatttg atcaaaatgc
    70741 actacgctta gattagaaca gacaaaaacc tctgctatca tgtcacaaat agtagaggtt
    70801 ttacactgca aaaagaggta tgaaaatcca ataacatagt tacaaggctt tcgtttaata
    70861 ccccccacta atagattaaa atgaaagact tcttacatta atagaagttt ctgttgcttt
    70921 tctcagaaaa atgtggttaa ataatcaaat attattaacg aaaaatgagt taatgattaa
    70981 cattaaactg gttttagtta aaacatttag gagggtatca ggtggagaaa aagcaacgtt
    71041 tttcccttag aaaatacaaa tcaggaacgt tttcggtctt aataggaagc gttttcttga
    71101 tgatgacaac aacagtagca gcagatgagc taaccacaac gagtgaacca acaatcacga
    71161 atcacgctca acaacaagcg caacatctca ccaatacaga gttgagctca gctgaatcac
    71221 aatccccaga cacatcacaa atcactccca agataaatcg tgaaaaagag caaccacaag
    71281 gtctagtctc tgagccaacc acaactgagc tagctgacac agatgcagca ccaatggcta
    71341 atacaggtcc tgatgcgact caaaaaagcg cttctttacc gccagtcaat acagatgttc
    71401 acgattgggt aaaaaccaaa ggagcttggg acaaggggta caaaggacaa ggtaaggttg
    71461 ttgcagttat tgacacaggg atcgatccgg cccatcaaag catgcgcatc agtgatgtat
    71521 caactgctaa agtaaaatca aaagaagaca tgctagcacg ccaaaaagcc gccggtatta
    71581 attatgggag ttggataaat gataaagttg tttttgcaca taattatgtg gaaaatagcg
    71641 ataatatcaa agaaaatcaa ttcgaggatt ttgatgagga ctgggaaaac tttgagtttg
    71701 atgcagagcc aaaagccatc aaaaaacaca agatctatcg tccccaatca acccaggcac
    71761 cgaaagaaac tgttatcaaa acagaagaaa cagatggttc acatgatatt gactggacac
    71821 aaacagacga tgacaccaaa tacgagtcac acggtatgca tgtgacaggt attgtagccg
    71881 gtaatagtaa agaagccgct gctactggag aacgcttttt aggaattgca ccagagaccc
    71941 aagtcatgtt catgcgtgtt tttgccaacg acgtcatggg atcagctgaa tcactcttta
    72001 tcaaagctat cgaagatgcc gtggccttag gagcagatgt gatcaacctg agtcttggga
    72061 ccgctaatgg tgcacagctt agtggcagca agcctctaat ggaagcaatt gaaaaagcta
    72121 aaaaagccgg tgtatcagtt gttgtagcag caggaaatga gcgcgtctat ggatctgacc
    72181 atgatgatcc attggcaaca aatccagact atggtttggt cggttctccc tcaacgggtc
    72241 gaacaccaac atcagtggca gctataaaca gtaagtgggt gattcaacgt ctaatgacgg
    72301 tcaaagaatt agaaaaccgt gccgatttaa accatggtaa agccatctat tcagagtctg
    72361 tcgactttaa agacataaaa gatagcctag gttatgataa atcgcatcaa tttgcttatg
    72421 tcaaagagtc aactgatgcg ggttataacg cacaagacgt taaaggtaaa attgctttaa
    72481 ttgaacgtga tcccaataaa acctatgacg aaatgattgc tttggctaag aaacatggag
    72541 ccctgggagt acttattttt aataacaagc ctggtcaatc aaaccgctca atgcgcctaa
    72601 cagctaatgg gatggggata ccatctgctt tcatatcgca cgaatttggt aaggccatgt
    72661 cccaattaaa tggcaatggt acaggaagtt tagagtttga cagtgtggtc tcaaaagcac
    72721 cgagtcaaaa aggcaatgaa atgaatcatt tttcaaattg gggcctaact tctgatggct
    72781 atttaaaacc tgacattact gcaccagggg gtgatatcta ctctacctat aacgataacc
    72841 actatggtag ccaaacagga acaagtatgg cctctcctca gattgctggc gccagccttt
    72901 tggtcaaaca atacctagaa aagactcagc caaacttgcc aaaagaaaaa attgctgata
    72961 tcgttaagaa cctattgatg agcaatgctc aaattcatgt taatccagag acaaaaacga
    73021 ccacctcacc gcgtcagcaa ggggcaggat tacttaatat tgacggagct gtcactagcg
    73081 gcctttatgt gacaggaaaa gacaactatg gcagtatatc attaggcaac atcacagata
    73141 cgatgacgtt tgatgtgact gttcacaacc taagcaataa agccaaaaca ttacgttatg
    73201 acacagaatt gctaacagat catgtagacc cacaaaaggg ccgcttcact ttgacttctc
    73261 gctccttaaa aacgtaccaa ggaggagaag ttacagtccc agccaatgga aaagtgactg
    73321 taagggttac catggatgtc tcacagttca caaaagagct aacaaaacag atgccaaatg
    73381 gttactatct agaaggtttt gtccgcttta gagatagtca agatgaccaa ctaaatagag
    73441 taaacattcc ttttgttggt tttaaagggc aatttgaaaa cttagcagtt gcagaagagt
    73501 ccatttacag gttaaaatct caaggcaaaa ctggttttta ctttgatgaa tcaggtccaa
    73561 aagacgatat ctatgtcggt aaacacttta caggactcgt cactcttggt tcagagacca
    73621 atgtgtcaac caaaacgatt tctgacaatg gtctacacac acttggcacc tttaaaaatg
    73681 cagatggcaa atttatctta gaaaaaaatg cccaaggaaa ccctgtctta gccatttctc
    73741 caaatggtga taacaaccaa gattttgcag ccttcaaagg tgttttcttg agaaaatatc
    73801 aaggcttaaa agcaagtgtc taccatgcta gtgacaagga acacaaaaat ccactgtggg
    73861 tcagcccaga aagctttaaa ggagataaaa actttaatag tgacattaga tttgcaaaat
    73921 cgacgaccct gttaggcaca gcgttttctg gaaaatcgtt aacaggagct gaattaccag
    73981 atgggtatta tcattatgtg gtgtcttatt acccagatgt ggttggtgcc aaacgtcaag
    74041 aaatgacatt tgacatgatt ttagaccgac aaaaaccggt actatcacaa gcaacatttg
    74101 atcctgaaac aaaccgattc aaaccagaac ccctaaaaga ccgtggatta gctggtgttc
    74161 gcaaagacag tgtcttttat ctagaaagaa aagacaacaa gccttataca gttacgataa
    74221 acgatagcta caaatatgtc tcagtagaag acaataaaac atttgtggag cgacaagctg
    74281 atggcagctt tatcttgccg cttgataaag caaaattagg ggatttctat tacatggtcg
    74341 aggattttgc agggaacgtg gccatcgcta agttaggaga tcacctacca caaacattag
    74401 gtaaaacacc aattaaactt aagcttacag acggtaatta tcagaccaaa gaaacgctta
    74461 aagataatct tgaaatgaca cagtctgaca caggcctagt cacaaatcaa gcccagctag
    74521 cagtggtgca ccgcaatcag ccgcaaagcc agctaacaaa gatgaatcag gatttcttta
    74581 tctcaccaaa cgaagatggg aataaagact tcgtggcctt taaaggcttg aaaaataacg
    74641 tgtataatga cttaacggtt aacgtatacg ctaaagatga ccaccaaaaa caaaccccta
    74701 tctggtctag tcaagcaggc gctagtgcat ccgctattga aagtacagcc tggtatggca
    74761 taacagcccg aggaagcaag gtgatgccag gtgattatca gtatgttgtg acctatcgtg
    74821 acgaacatgg taaagaacat caaaagcagt acaccatatc tgtgaatgac aaaaaaccaa
    74881 tgatcactca gggacgtttt gataccatta atggcgttga ccactttact cctgacaaga
    74941 caaaagccct tggctcatca ggcattgtcc gcgaagaagt cttttacttg gccaagaaaa
    75001 atggccgtaa atttgatgtg acagaaggta aagatggtat cacagttagt gacaataagg
    75061 tgtatatccc taaaaatcca gatggttctt acaccatttc aaaaagagat ggtgtcacac
    75121 tgtcagatta ttactacctt gtcgaagata gagctggtaa tgtatctttt gctaccttgc
    75181 gtgacctaaa agcggtcgga aaagacaaag cagtagtcaa ctttggatta gacttaccgg
    75241 tccctgaaga caaacaaata gtgaacttta cctaccttgt gcgggatgca gatggtaaac
    75301 cgattgaaaa cctagagtat tataataact caggtaacag tcttatcttg ccatatggca
    75361 aatacacggt cgaattgttg acctatgaca ccaatgcagc caaactagag tcagataaaa
    75421 tcgtttcctt taccttgtca gctgataaca acttccagca agttaccttt aagatgacga
    75481 tgttagcaac ctctcaaata actgcccact ttgatcatct tttgccagaa ggcagtcgcg
    75541 ttagccttaa aacagctcaa ggtcagctaa tcccgcttga acagtccttg tatgtgccta
    75601 aagcttatgg caaaaccgtt caagaaggca cttacgaagt tgttgtcagc ctgcctaaag
    75661 gctaccgtat cgaaggcaac acaaaggtga ataccctacc aaatgaagtg cacgaactat
    75721 cattacgcct tgtcaaagta ggagatgcct cagattcaac tggtgatcat aaggttatgt
    75781 caaaaaataa ttcacaggct ttgacagcct ctgccacacc aaccaagaca acgacctcag
    75841 caacagcaaa agccctacca tcagcgggtg aaaaaatggg tctcaagttg cgcatagtag
    75901 gtcttgtgtt actcggactt acttgcgtct ttagccgaaa aaaatcaacc aaagattgat
    75961 aacaaaacgc aaagagacaa caaatcatcc cttttaacag tcggaccttt ctttgagatt
    76021 gagctgacag atgatcattt cttaacaaaa gttaaagagt tgagcaaagt aaggaacaga
    76081 cataaagacc ataaaagcaa gccagtttgg cttgctttta tggtgttatt gtcgtgtgct
    76141 acgatgactg aaaaagaaca acgaacaatg acctcgaatc accaaaagga cttgatttga
    76201 tagcttccct atgctatact aagcctattc tttcaagaag gactcctttt gtggaaaaga
    76261 ctgaaaaaaa ggacactaat ctatccaaaa actccagatg agtagctgca aacggatgcc
    76321 tctgctgtga tcaaggggat aaagcagact ggcttgtcac tacctgagca ggtgtcaaaa
    76381 aaagaagctt ttggctttga cattcaacat actgagcttt agtattttag aacggagaca
    76441 cccccagttt tgtactcaaa aagcatgaac agttctgctg atgtgacatc tgatttctac
    76501 gtgcgcagtg agccaaaaca gctgctgttt gctatggaaa aagtggcgcc aatcgagatt
    76561 tttgagaccc taggctcaaa aataaggcat agaattccaa aggaagtcgc tgccgtgagc
    76621 acaacctaag aaaaactatt ccaaaagact ttttctgcaa tttgaaatac tgaatttcag
    76681 tattttttct tttgtaacta agagcaatga atgaggtaaa aaaaggcaac aatgtcaaaa
    76741 aagaaacgaa aacactatca ggtttatgat ggattacgct gtgctatgac gctttgtttt
    76801 atcagtggct atgtcaatgc ctttacctat atgacccaag gcaaacgttt tgcaggtgtt
    76861 cagacaggca accttttgtc atttgccatt cgcttgtcag aacagcaact gaaggaggca
    76921 ctgcaatttt tacttcccat gatagttttt atgcttgggc agtcctttac ttatttcatg
    76981 caccgctggg ccactaaaaa agggctccac tggtacttat tatcaagcgt tatcttaaca
    77041 gggattgctt ttggaatggc cctattcacg ccttttcttc cttccaatgt tacggtagca
    77101 gctttagcct tttttgcctc tattcaggtt gatacgttta aaaccttacg aggagctagc
    77161 tatgctaatg tgatgatgac aggaaatatc aaaaatgccg cctatttact aaccaaaggc
    77221 ttgtacgaaa aaaatcacga actaacacat atcggacgca acaccttaat agtcatcttg
    77281 gcttttgctg ttggggtggt ttgttcgacg ttgctttgca tagcttatgg cgagtatgct
    77341 ttgatgccta ttttaatgcc tttactctat gtcaattacc tgttggctca ggaattttat
    77401 cacattcaaa ccaaaatcaa accgattaaa agaacttaac ggacttgtgg agaaagaaag
    77461 ttcatttagg gtaagttttt acttaccctc cgttgatttg ctggttttgc tgccccttaa
    77521 aatatgatat aatagaccat tgatagctgt tacttaacag aaggctgata aaaaaggttc
    77581 cattagcctt gagggttgaa tttaaacagg aaaaaacgaa atcctacact atgcatctca
    77641 atcgtacttt catttgagta tggttctgaa aggaaactat gatgaaaaaa ccattttaca
    77701 ttacgacacc catttattac ccatctggga aattacacat tggttctgcc tatacaacta
    77761 ttgcttgtga tgtcttggca cgctacaagc gtctgatggg ccatgaggtc ttttacctca
    77821 caggactaga tgaacacgga caaaagattc aaaccaaggc aaaagaagct ggtattacac
    77881 ctcaaaccta cgttgataac atggcaaagg atgtcaaagc cctttggcaa ttgctagata
    77941 tttcctatga cacatttatc cggacaacgg acgattacca cgaagaggtc gttgctgctg
    78001 tttttgaaaa attactggcg caagatgaca tttaccttgg tgaatactct ggttggtatt
    78061 ctgtctctga tgaggaattt ttcactgaaa gccaactaga agaagttttt agagatgaag
    78121 acggtaaggt tattggaggg attgctcctt caggtcacca agtagagtgg gtatcagaag
    78181 aatcttactt cctacgcctt agcaagtacg ctgaccgcct cgtagccttc tttaaagagc
    78241 gacctgactt tatccaacca gacggtcgta tgaatgaaat ggtgaaaaac tttatcgagc
    78301 caggcctaga agatttggct gttagccgta ctacctttac atggggtgta cctgttccat
    78361 cagacccaaa acacgtggtt tatgtctgga tcgatgccct cctaaactac gcgacagctc
    78421 ttggttatgg tcaagctaat cacgctaact ttgataaatt ctggaatgga acggttttcc
    78481 acatggttgg aaaagatatc ttgcgttttc atagcatcta ctggccgatc ttactcatga
    78541 tgcttgattt accaatgcca gaccgcttaa ttgcccatgg ttggtttgtc atgaaagacg
    78601 gtaaaatgtc taaatcaaaa ggcaatgtgg tctatccaga aatgttagtg gagcgctttg
    78661 gacttgatcc tcttcgttat tacctcatgc gttctttgcc agttggttca gacgggacct
    78721 ttacccctga agattatgtg ggtcgtatca actatgagtt agccaacgac cttggcaatt
    78781 tattgaaccg taccgttgcc atgatcaaca agtattttga cggcacggtg ccagcttatg
    78841 ttgacaatgg aactgccttt gatgctgact tcagtcaact gattgatgct cagctagcag
    78901 actaccataa gcacatggaa gctgtagact acccacgcgc cttagaagcc atttggacca
    78961 tcattgctcg taccaacaaa tacatcgatg aaaccgctcc ttgggtgctc gctaaagaag
    79021 atggtgacaa agctcaacta gccagtgtca tggcacactt ggcagctagc ttgcgcgtgg
    79081 tcgctcatgt gatccaaccc tttatgatgg aaacatcagc tgctatcatg gcccagcttg
    79141 gcttagcgcc tgtttctgac ttatcaacac ttgctctagc agattttcct gccaatacaa
    79201 aggtcgttgc caagggcaca ccgattttcc cacgtcttga catggaagct gagattgact
    79261 acattaaagc ccaaatgggt gacagctcag ctatttccca agaaaaagaa tgggtccctg
    79321 aagaggttgc gctcaaatct gaaaaagacg tcatcacatt tgaaaccttt gatgctgtgg
    79381 aaatccgtgt ggcagaagtc aaggaagtgt caaaagtgga aggttcagaa aaattgctgc
    79441 gctttagagt agatgcagga gacgggcaag accgtcaaat cttgtcaggt attgccaaat
    79501 gctaccccaa cgagcaagaa ttagtgggta aaaaactaca aattgttgct aacctgaaac
    79561 cgcgtaaaat gatgaaacaa tacatcagcc aaggcatgat tttgtcagca gagcatggag
    79621 atcagctaac ggtcttaacc gttgattcct ctgttccaaa tggaagtatc attggctgat
    79681 gctgacctat tattcaaaaa cctaagcaag aatatgtgct taggtttttt gattgccttc
    79741 aagaaagatg aataacgaga tgggtgatat ggcatcaaaa gcataactat cctaggacct
    79801 gtcctagtaa gattgctgtg cttttgacaa gtgaacagag aggtcttaaa ctatatcttg
    79861 tcataaaaaa gattgcaaaa cactatatct tgtgttatta tggtttttat aaaagttaag
    79921 cgtgcttaac tttagccaaa caaaagagga cactagagcg tgtcccacaa gacagttgag
    79981 cccttcgctt ttttagtaga gtaggagtgc tcaagtcaaa aagagacagc ccatcaaacc
    80041 attgctgttt agcaggtctt tttgtgttgc tcaaaaaaca gttcctgtct gtttggcgtc
    80101 tagtgatgac ttaggcttag cgctactaga cttttgtttg aaaaatacct caagccttga
    80161 aggagacaaa tgacccaaca ttattatgaa cgttcgcaat ccccaattga atacgcccta
    80221 tctgaaacac aaaaacagtt gcgctccatc aattggaatt accttaatga tgacaaagat
    80281 ttggaagtgt ggaaccgtgt cactcaaaat ttttggttgc ctgaaaaggt tcctgtttct
    80341 aatgatttaa attcttggcg ctccttgggt gaggattggc aacagctcat cacaaggacc
    80401 tacactgggt tgaccttact agatacagta caagcaacgg ttggtgatgt ggcacaaatt
    80461 cagcattcac aaaccgacca cgaacaagtc atctatacga actttgcctt tatggtgggg
    80521 attcatgccc gctcttacgg gacgattttt tcgacccttt gcagcagcga gcaaatcgaa
    80581 gaagcacatg aatgggtggt ctcaacacag agcttgcaag accgttcccg tgtcttgatt
    80641 ccttactata cgggtgatga ccctttgaaa tcaaaagtag cagcagctat gatgccagga
    80701 tttttgctgt atggcggctt ttaccttcct ttttacctat cagcacgtgg taaaatgcca
    80761 aacacctctg atatcatccg cctgatttta cgcgacaaag ttattcacaa ctattacagc
    80821 ggttacaaat accaacaaaa agtagcacgt cttagcccag aaaaacaggc tgaaatgaag
    80881 gcctttgtct ttgacttgct ctatgaactc atcgaccttg aaaaagctta cttgagagaa
    80941 ctgtatgcag gctttgattt ggcagaagat gctatccgtt ttagccttta taacgctgga
    81001 aaattccttc aaaatttagg ttatgattcg cccttcacag atgaagaaac ccgtatctca
    81061 cctgaagtat ttgctcagct atcagcgcgt gcagacgaaa accatgactt tttctcaggc
    81121 aatggctcat cttatgtgat ggggatcacc gaagaaacca cagatgacga ttgggagttt
    81181 taagaaatgg cagagctgat tattgtgtat ttttcatcta agtccaataa cacccatcgc
    81241 ttcgtccaaa aattaggcct gcctgctcag cgcataccag tagataatag gccacttgaa
    81301 gtttcaaccc attacctttt gattgtgcct acctatgcag ctggtggttc agatgccaaa
    81361 ggagccgttc ctaaacaagt gattcgtttt ttgaataatc ctaacaatcg taaacattgt
    81421 aagggagtga tctcttcagg aaataccaat tttggcgata cctttgcctt agcaggccct
    81481 atcatttctc aaaaattgca ggtcccccta ttacaccaat ttgaactatt aggcactgct
    81541 acagatgtca aaaaggtaca ggcgatcttt gcccgcttaa agcatcacac acacgacaag
    81601 caaaaacaaa tcaacaacct gattacagaa aggacacacc catgtcacaa accaatgcgt
    81661 catacctctc attaaatgca ctgacccgtt ttaaaaaacc agacggcagc taccattttg
    81721 atagcgataa agaagctgtt aggcgctacc tggaagaaca tgtctctccc aatcaaatgg
    81781 cttttaactc actagaagat aagctagctt acctcatcaa tgaagggtat tatgagcagg
    81841 ccatttttga tgcctatcct aacgacctta tcaaggaggc ctttcattat gcctatcaac
    81901 aaggctatcg ctttttaaat ctcatgggcg ctatgaaatt ttaccagtcc tatgccttaa
    81961 aaaccttgga cggtaagcag tatctagaaa catttgaaga ccgtgctgtg atgaatgcac
    82021 tctttttagc tgatggagat caaacctttg tctttgacgt gattgatgcc atcttgcacc
    82081 gacgctttca accagctacc ccaacgtttt taaatgccgg aaaaaaacgc cgtggcgaat
    82141 acatttcgtg ctatcttctt cgtgtcgaag acaatatgga atctatctct cgcgccatta
    82201 gcaccagctt gcaactctca aaaagaggag gcggagtcgc cttgtgcctc accaacctca
    82261 gagaaatcgg agcaccgata aaagggattg aaaatcaggc aacaggcatc gttcctgtca
    82321 tgaaactact agaggactct ttttcctatg ccaatcaact aggtcagcgc caaggagcag
    82381 gtgcggttta ccttcatgcc catcatcctg aggtcttgac ctttttagac accaaacgtg
    82441 aaaatgccga tgagaaaatt cgcattaagt ccctagcatt gggacttgtg atcccagata
    82501 ttaccttcca gttagccaaa gaaaacaaag acatggctct cttttcaccc tatgatatca
    82561 agcgtgccta tggcaaggac atgtcagaca tctccatcac agaggaatac gataaactgc
    82621 tagccaatcc agccatcaaa aagacctaca tcagtgctag aaagttcttt cagttaattg
    82681 cagagctaca ttttgagtca ggctaccctt acctcttgtt tgatgacacg gtcaataagc
    82741 gcaatcctca tgccaaaaaa ggcagaatcg tcatgtctaa cctgtgttct gaaatcgctc
    82801 aagtcagcac cccaagcact ttcaaagaag atttgagttt tgaaaccatt ggagaggata
    82861 tctgctgtaa tttagggtct atcaatattg cccaggctat ggcagatgcg ccacactttg
    82921 aacagctcat cacaacgagc atcagagcac ttgatcgtgt ctctagagtg tctgacctga
    82981 attgtgcgcc ttctgttgag acaggcaatg cagccaacca tgcagtagga ttaggcgcca
    83041 tgaacctaca tgggtttttg gcaaccaacc acatctacta tgatacaaaa gaagccgttg
    83101 actttacaga tctgtttttc catgccatgg cctattatgc ttttaaagcc tcttgccaat
    83161 tagccaaaga aaaaggcgct tttgcaggat tttcgttatc aacctatgca gatggcactt
    83221 attttgccaa gtatttgcaa gaagatgcca agccacaaac agccaaggtt gccacattgt
    83281 tgcaagatta cggcttcaca ttgccaacgg tggcggattg gcaagcccta gtagcagata
    83341 tcaagcagtt tggtctagcc aatgcgcact tgctagcggt tgctccgaca ggctccatca
    83401 gctacttatc atcctgcact ccgagtctac aaccagtcgt tgctcctgtt gaggtccgaa
    83461 aagaaggtag cctgggcagg atttacgtcc ctgcctatca gattgaccag gctaactatg
    83521 cttactatga acgtggtgcc tatgaggtcg gcccaaaagc catcatcgac gttgtagctg
    83581 ctgcccaaaa acacgttgac caagccatct cattgaccct gtttatgacc gatcaagcga
    83641 ccacacgtga ccttaaccgc tcctatatcc aagcttttaa gcaaaactgt gcatcgatct
    83701 attatgtgcg agtgaggcaa gacgtcctag caggcagcga acagtatgac gaggatagtc
    83761 ttgtgacagc tcctggagct agtgatgaga ccaccacaga gtgtcaaagc tgtatgatct
    83821 aacagctact agaaagctct cgtgtcccct atcacatgca taggatcagt gcactcgacc
    83881 tttcaagaca agcaagcatc agctcttgct tgtctttttt tggccttaaa gcccgttagt
    83941 ctgctgctat gcgaggcttt ttttgagcat tagaacgtca aaaaaaagga catggagtcc
    84001 ttttttggtg atcggtgttg aggccgtcac aaactgccct tgaaatacgc ttctatgtgg
    84061 agcttttttt ggtcttgtga cacgtaagct ccaacgacct ccagctgaca gcctcttgga
    84121 aacaagagct caacctctga aggcacagcc gaataaggct cgacaaaggc tgccttggcc
    84181 ccttttttga cacagatgcg cacctccacc ggtcgatggg tcatggcgcc gtttttcaag
    84241 gctgtcgtgc tcataaaact gtgcttggtg tagcgtgtgc caagcttgat cttacaaagg
    84301 atatgagggt caaactgatg gttacggtag taagacgtga gatcagcgtg tgaaacaccg
    84361 atatcacgca aaaacgtctc gtacacatag cggtatacca caatgttcca cgggatgact
    84421 agccggtgag tcgcagcatc gagctgggcc acctgatccc ttagctcagg cgtgagttgg
    84481 ctgagctcac ctttggtctt gtctaggctg gtgttgatag gggctgcctg ctggcaggta
    84541 taagcagcca tcgcctcttg ctcagccctt gggtagacga gacccttgcc ccagcgcttt
    84601 ttaatcagtg tctcagcgcc cttttgatcc gaaaaggtaa aataagcagg cgtcccctca
    84661 caaaccgccc gctcatagtg ctcacagaca aaaagctcga ccaagccctc agtttgccag
    84721 actagactaa aacagctaag aagaaggatc atagccagtt gatagcgctt ttttagcata
    84781 gttccccctg acaccagccc aaaaggctga cccttcccct aggccttttg gctagctcat
    84841 gataccacca gtttctcaga caggctaatc tttttgtcat ctctttctcc ctaaacagac
    84901 tctcttatca tgatattatg ctattatgag cgatttgtca atccatagca accgtttttt
    84961 agccagataa gtaagctttt gacctagagg cagacaagag tggcagtacc tgccgtttgc
    85021 tgtggtggct aataaggact aacataaaat atcaaacgct ttcaaaaaca gctcctaagc
    85081 ttaaaaaagc tgatgaacaa ttttttgagt ttaagagaga ttgccatgat tttctaatca
    85141 tttgatgttt aaaatgataa aaacatgaac ttgaaatgaa agaatacgag tagtaaacta
    85201 aaaatatatc aaaaataatg aaatggagtg attttatgaa aacaaaatca aaacgctttt
    85261 taaacctagc aaccctttgc ttggccctac taggaacaac tttgctaatg acacgtcccg
    85321 taaaggcgga ggtgtcagaa attgggcacg atcacgtaac aatcagttct aatggtcaaa
    85381 cagatgaggg tgctgcatat ggtagaggcc atgatgatgg aagtaagttt ggatatgaag
    85441 caggattaca atcgagttgg aatgagtctg aacccccatc atcagataaa atacctgagc
    85501 ctagtgtgaa tccatatgaa agtagtaatg aacaagatag agaggattat aaagaaggtt
    85561 ttcgtgatgg atatcctggg ggctatgtcg caggctggag aaaaactcat ccaatagaag
    85621 ctactcttca atatttatgg tatacggtat ctagttggtt tgaaagctta tttaataata
    85681 gtaagtagat atttgtttgt tgattaaagt tacttaaggt aaaataaaat gtctgtcagc
    85741 aacttcgtgc tagtggcttt tgttttacct ttcccacact aatctgctat aattgggata
    85801 atccctttag tagtcaggcc acctctgata ggggtggcct tttttgggtt ttagtgaccc
    85861 gtgtgttgaa aaaggagcat ttatgccaga aaagaaagtg ttgcccgtcc taagcatggt
    85921 ggtgagtttt gtctgcctgc ttgatcatat cttctttttt gggctctttg tcaactgtag
    85981 tgggtgactt atagctaaac acgtgaggag acgagactcg tcttctcttt tgttatgttc
    86041 aaagcgaggt aaatgcgttt tttaaagtta tcaaagttcc gaaagccaaa agcattgcgt
    86101 ttgatgtgtt tgatgagttt attggttgct tctagtttgg cgtttgaaca aggaagctca
    86161 atgacattag tgacatagtt tttgtatcgc atcagggtgt tcaaagccgt tttaaagatg
    86221 ttattgagca gaagtaacac cttttgaatt aagtcaaaaa atgctcagta ttcttctctt
    86281 gtaagtgaaa aaggagtaac tgataaaggt catagtaatc ttttaattca ggaacgggat
    86341 taaatagttt attgagacat tgtttaggag ttagcgtttc cctaaaggtt ctagagtaga
    86401 aggcgttgag cgagagtttt cggctacctt ttgaatgagt tgacagtagt atttaagaac
    86461 tctgtattcc agagactttt tgtcatattg cttcatgatg tgaatccggg tttgactgag
    86521 cgctctgttc atgtgctgaa caataggaaa cgatctagga cgatgttagc ttgaggaaat
    86581 agctttttaa tgagcgaaat atagcttcct gacatatcaa cggtaacgat tttgacactg
    86641 tttctagcct ctttagagta tctaaagaag tgatttcgaa tggttgtttg tcgccggttg
    86701 tccagtatgg agatgatttt tttggactca aaatcttgag cgataaaggc gagttcgccc
    86761 ttttgatagg aaaatccatc ccaagacaac acctcgggta gtttagagaa gtcttccttg
    86821 attacaaact ggttgagttt tcggtagacg gtagaagatg atatagccga ctgttgagcg
    86881 atgttagtga gagctcctcg attaaccaag gtttgtgcga ctttttgtct gaggggctca
    86941 gagatctgac agtttttttg aaccagactt gtttccgcta cagccacttt tcgacaggat
    87001 ttgcattgag atcttctctt ttttaaacga atgagactag gaaaaccacc aatttcgata
    87061 aaggggattt tagaatgttt ctgggagtcg tatttaatct gaactccttt acagtgttta
    87121 cacttaggta gggatagtca agtgtggcga agacttcgat aagtgtctcg cgttgaaaaa
    87181 ccttgttcaa ggtgatgtgt ttatctttaa ttctaatcaa ttctgtagta tgatagagtt
    87241 gtctcatatg attctttcta atgatggttt ggtcgctttg cattataagt catatgggat
    87301 tttttgctac ttatagttac ctctacttgc cgtttgtagc aagtagtcta gcattgtagg
    87361 tcttgtataa aacaaaagca agtgttcgct ggctgggctt gtagtagcag taacagtata
    87421 atatgaaccg acagctattc cctattaaaa tagacgcaaa tgtccacgct ttcactgtag
    87481 ttgctagtaa ggtctaacac aaaacatcaa aggctttcaa aaaaactcct aagcgcaaaa
    87541 aggcggacta acagccccaa gcaagcactt tctgaggtaa taagagattg ccatgatttt
    87601 ctaatcattt gatgtttaaa atgataaaaa aatgaacttg aaatgaaaga gtgcgagtag
    87661 taaactatga ccgtatcaaa aataatgaaa tggagtgatt ttatgaaaac aaaatcaaaa
    87721 cgctttttaa atctagcaac cctttgcttg gccctactag gaacgacttt gctaatggga
    87781 caacccataa aggcggaggc agtagtatct gatttgaatg atgcagtctc ttcgatggga
    87841 gatacagcag aggaaactga cgtcaatatc gatggtcaag aagacgaaga agacgaagaa
    87901 gacgatgctt atatgcaagg gagaagactg ggagagaaag aggggtatga agctggtcaa
    87961 aaagacaacg ctcccagatt atttgacagt agtacgctag agccccctac taatccttac
    88021 gaggatgcgg gtgatacatt gaatagtaat tggtttaaaa gtggttacag cgatgcttat
    88081 ggtcaagcat atgagcaggg atggaatgat actcatccga tacaatcaac tttaatttgg
    88141 ctgtggggga tagtaaggaa cttatttaga ttttagattt aggtgacggt taccaacaac
    88201 aaagtaatta atattacgtt ataaaaagct agtatcgctt ttgaggatag tgttatttac
    88261 tcttttgaga tatttgaaat actataacac taaaatgaat ccttttatca caagcatttc
    88321 ccccactaat ctgctataat tgggatagtc cctttagtag tcaggccacc tctgataggg
    88381 gtggcttttt tgggttttag tgacccgtgt gttgagaaag gagcatttat gccagaaaag
    88441 aaagtgttgc ccgtcttaag catggtggtg agttttgtct gcctgcttga tcatatcttc
    88501 ttttttgcta cttatagtta tctctacttg ccgcttgtag caagtagtct agctcttatc
    88561 agtctggtgc aaaacaaagg caggcaaaag gtttggtcgc tagctgggct tatcttagct
    88621 gctctagccg tggcagtagt ggcctatgcc tactatgacc caaccgtcat cccctattaa
    88681 ccttatctcc tctttgcacg caatcagcag cacaaaaagc acccttacag gtgcttagat
    88741 tgaaaaaggt cttttgtgat actttttctg aggcggtgcg gacggtagcg acttcctttg
    88801 gaattccctg acttattttt gagacctaag gtctcaaaaa tcccgagtgg cgtcctctcg
    88861 tattgacgcc actttcacca tgacaaaaat catctgtttt ggctcccttt gtgtgtaaaa
    88921 atcggaaacc gtatgtcaca tcagcagagc tgtttatgct ttttgaacat agaactaggt
    88981 ttgtctaggt tctaagcacc tgtaagggtg ctttttgtac tttagccaac ctcctcatag
    89041 cctagcaaac cctcactgaa acagtccctt aaagttccac caagcgtctt gaaaaacatc
    89101 gcgtacttta tcccgccatg ttttttgttc ctctcttaga cgagaatttt caaaaaggtt
    89161 ttcggcatat tcttcatcaa atgctttata ttgttcattt ttgagtgttt ctgaatcaat
    89221 aaaagtcatc attccagcta aaaatccatc atctccttga aaataaatag taaattcttt
    89281 tggaggttgc gacgttgaga tatcgagagt gactgtagta gtcactgaat tgctgtttcg
    89341 ttgtttcgtg tttttatcaa tccatgcaga ccatcctgga ggaagtgtaa cagtgacttt
    89401 attacctgta atgtgaacag gaatttcatt tctgttatca tctaaaactt tttgttgtct
    89461 tggaagaggt acccaagaac tctcctccgc tctgacgcta ctagttaccc cgatggtaaa
    89521 gagtgccagt agcaaagtta atgtcttttt cattttttcc tctccttttg ctcatttttt
    89581 aagacaaggg ttattatacc aattcttgcg ctaaaaagat agggagttca tgtcttgtgg
    89641 ttgggaaatg gcgcgactat tcttggtagg atcagctagt ttttttccta gtcagtcgct
    89701 agctgttgcc attttacctt taaaggtcgt ttgggtatta tgtcatattt tatgagcggt
    89761 tttatatttg gtatcaatat cttatagggc aattg
//
LOCUS       DATXQK010000003        81889 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.003, whole genome shotgun sequence.
ACCESSION   DATXQK010000003 DATXQK010000000
VERSION     DATXQK010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 81889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81889
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.003"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            127..2325
                     /gene="nrdD"
                     /locus_tag="VC394_000176"
     CDS             127..2325
                     /gene="nrdD"
                     /locus_tag="VC394_000176"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEP5993678.1"
                     /translation="MVSLEEDKVTVQPDIKVIKRDGRLVNFDSTKIYSALLKASMKVT
                     RMSPLVEAKLEAISDRIIAEIIERFPTNIKIYEIQNIVEHKLLAANEYAIAKEYINYR
                     TQRDFARSQATDINFSIDKLINKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLKM
                     LPSHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLANGFKIGNAEVESPKSIQT
                     ATAQISQIIANVASSQYGGCTADRIDEFLAPYAELNFKKHMADAKKWIVETKRESYAF
                     EKTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSWFEREIQKAILTIRINGL
                     GSEHRTAIFPKLIFTVKRGLNLEPDSPNYDIKTLALECATKRMYPDMLSYDKIIDLTG
                     SFKSPMGCRSFLQGWKDENGQDVTSGRMNLGVVTLNLPRIAMESNGDMDKFWELFNER
                     MLISKDALIYRVERVTEAKPANAPILYQYGAFGKRLEKTGNVNDLFKNRRATVSLGYI
                     GLYEVASVFYGGQWEGNPDAKAFTLSIIKAMKQACEDWSDEYGYHFSVYSTPSESLTD
                     RFCRLDTEKFGIVTDITDKEYYTNSFHYDVRKSPTPFEKLDFEKDYPEAGASGGFIHY
                     CEYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDKCYNCQFEGDFTPTERGFTCPNCG
                     NNDPKTVDVVKRTCGYLGNPQARPMVNGRHKEISARVKHMNGSTIKYPGL"
     gene            2507..2653
                     /locus_tag="VC394_000177"
     CDS             2507..2653
                     /locus_tag="VC394_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5993679.1"
                     /translation="MGKYQLDYKGMQQVERFHEKHSKKKTDKKSRVQELKARFLEKSK
                     KQDN"
     gene            2682..3617
                     /locus_tag="VC394_000178"
     CDS             2682..3617
                     /locus_tag="VC394_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEP5993680.1"
                     /translation="MLNIGIVGLGAISQKAYLPYMRQLSDITWHLSTRNAAVRQQVGQ
                     LFGHAILYSDVKELSKASLDGVFIHAATSAHAELASLFLNQGIPVFMDKPIADNYLMT
                     KNLYDLAKENQTFLMAGFNRRFTPRVKKLSSLSTKRKVAVEKNDLNRPGDMTFKLFDF
                     FIHPLDTALFLSEGTLLKGHFQYHLEAGLLSQVMVTLMTESMTTTASMNLQSGSRREV
                     MEVQRAEETYHLENLDELSIYKGTEKRVLGFASWDTTLHKRGFETMIDAFLEAISTGV
                     NPVSPESSLLSHWICQQIADSQLSYGELTVELPKD"
     gene            3626..4135
                     /locus_tag="VC394_000179"
     CDS             3626..4135
                     /locus_tag="VC394_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP5993681.1"
                     /translation="MEIRRPTLKDKDAVLSMINEFLEQKSATDGLWHFNVNDFNYETW
                     LEDSLRQEMGLSSQGVPAIQYVAFDERSQAIGFLNLRLRLNERLLEKGGHIGYSVRPS
                     QRGKGYAKEMLKQAVSYAISKNITTILVTCDETNVASRAVIVANGGVLEDSRGGTERY
                     WIEEQRGDK"
     gene            4135..4749
                     /gene="nrdG"
                     /locus_tag="VC394_000180"
     CDS             4135..4749
                     /gene="nrdG"
                     /locus_tag="VC394_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEP5993682.1"
                     /translation="MAEKCWNNPKPKEWQAEELSQGRIIDYKAFNFVDGEGVRNSLYV
                     SGCLFHCKGCYNAATWSFKAGMPYTQELEEQIMTDLAQPYVQGLTLLGGEPFLNTGIL
                     IPLIKRIRRELPEKDIWSWTGYTWEEMMLETPDKLEMLSLIDILVDGRFDITKKNLML
                     QFRGSSNQRIIDVQKSLAAKEVIIWDKLNDGDQTFEQISREDLL"
     gene            complement(4923..5654)
                     /gene="yaaA"
                     /locus_tag="VC394_000181"
     CDS             complement(4923..5654)
                     /gene="yaaA"
                     /locus_tag="VC394_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEP5993683.1"
                     /translation="MLTFLIPTAKEMMIPKESHPHLLPQDSQAILKIMAAMTTEDLAK
                     SYRIKEEAAKKEQQRWQDMASQQNLAYPAYQLFNGLMYRHIKRDKLTTQEQAYLTQQV
                     YITSSFYGIIPANHPIAEHRHDFHTRIKIEGQSLKSYWRPCYNQFAKEHPQVISLLSS
                     EFDDVFSKDCKQLWISPKFMAEKEGQFKTHSTISKKARGAFLTACMENNCQTVDSLKS
                     LVFAGFYYHPDLSTDHEFVYIKKEA"
     gene            complement(5696..6553)
                     /locus_tag="VC394_000182"
     CDS             complement(5696..6553)
                     /locus_tag="VC394_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEP5993684.1"
                     /translation="MTYPYNSTISLGTVSLNVTDLAKMTTFYTSIIGLQVLSQDTTSR
                     QLTTDGKTVILELRQTPLPANKAYGLYHTSFLVPDRHSLGLVLNHFLTRGISLEGSAD
                     HGYSEAIYLSGPEGNGIEIYHDKAVEHWDIRDNGQIIGVTEPTDTKSILEQLTDIPKR
                     FLLAQDTRIGHVHLSVKNALASSLLYQKVFDLGDKMTIPSASWIASGNYHHHLAFNHW
                     SAPYLKKHQEGAPGLAFLTIHIETPLLFSATLKKARLHGLAILQEDSSSFTTEDEEGI
                     CVNVILGQD"
     gene            6740..7077
                     /locus_tag="VC394_000183"
                     /pseudo
     CDS             6740..7077
                     /locus_tag="VC394_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018307.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(7356..8069)
                     /gene="treR"
                     /locus_tag="VC394_000184"
     CDS             complement(7356..8069)
                     /gene="treR"
                     /locus_tag="VC394_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEP5993685.1"
                     /translation="MTKYERIYKDLETKINKDFYKEGDFLPTEIELSQQYQASRDTVR
                     KALSLLTKAGLILKKQGRGTQVIKHHQIMFPISELTSYQELVSYSNLDSKTNVIAIDK
                     LIVDETLSKLTGFSKNSLVWRVTRQRVVEGVASVLDIDYLSKTLVPIMTREIAEHSIY
                     QYLEKELHLAIDFALKEVTIDQITDRDKILLDLGSDQHVVSVKSKVYLSNNNQFQFTE
                     SRHKLEKFKFLDFARRRPK"
     gene            8280..10304
                     /gene="treP"
                     /locus_tag="VC394_000185"
     CDS             8280..10304
                     /gene="treP"
                     /locus_tag="VC394_000185"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEP5993686.1"
                     /translation="MGKFEQDAKSLLTAIGGKENIKAVTHCATRMRFVLNDNNKANVK
                     EIEKISVVKGTFTNAGQFQVIIGNDVPVFYNDFTAVSGIEGVSKEAAKSAAKSNQNAL
                     QQVMTMLAEIFTPIIPAIIVGGLILGFRNILESVPFEFLGQQVEKGKLVFDAAGDPVW
                     NTIVRVSPFWSGVNHFLWLPGEAIFHFLPVGITWSVTRKMGTTQILGTVLGICLVSPQ
                     LLNAYAVAGTPAAEIAKNWVWDFGFFTINRIGYQAQVIPALLAGLSLAYLEIFWRKRI
                     PEVVSMIFVPFLSLIPALILAHTVLGPIGWTIGKGISFVVLAGLTGPVKWLFGAIFGA
                     LYAPLVITGLHHMTNAIDTQLIADTATRTTGLWPMIALSNIAQGSAVFAYYLMNRHEE
                     REAEISLPAAISAYLGVTEPALFGVNVKYVYPFVAGMIGSGIAGLLSTTFNVQANSIG
                     VGGLPGFMAINVKYMIPFFICMVVAIVVPMFLAFFFRKSHIMTKTEDEAKLPETPVSD
                     APVATAPHKTMQGTVITLTSPLTGEVKALSEAVDPVFAQGVMGQGALLQPTEGVLVAP
                     CDAEVSVLFPTKHAICLVTTEGLELLMHIGMDTVNLDGQGFEALVKQGDQVKAGQTLI
                     QFDIAAISEAGYATETPLVVTNQDAFTVTVEGSLPRQIKANDKLAVAMKK"
     gene            10372..12000
                     /gene="treC"
                     /locus_tag="VC394_000186"
     CDS             10372..12000
                     /gene="treC"
                     /locus_tag="VC394_000186"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEP5993687.1"
                     /translation="MTIDKKKVVYQIYPKSYKDTTGNGVGDLRGIIDKLPYLQELGID
                     MIWLNPFYPSPQRDNGYDVSDYTAVNPDFGTMADFEDLVKAAKEHQIELMLDMVLNHC
                     STDHEWFQKALAGDPYYQDFFILRDQPTDWVSKFGGNAWAPFGDTGKYYLHLFDVTQA
                     DLNWRNPHVREELAKVVNFWRDKGVKGFRFDVINLIGKDEELVDCPVNDGKPAYTDRP
                     ITHTYLHDLNQASFGQDDSFMTVGEMSATTIDNCLLYTAPEREELSMAFNFHHLKVDY
                     ENGQKWTIMAFDFAALRDLFHAWGEGMSQGNGWNALFYNNHDQPRALNRFVDVTHFRN
                     EGATMLAASIHLSRGTPYIYMGEEIGMLDPDFDSMDDYVDVESLNAYSSLLVSGKSAE
                     EAFAIIKAKSRDNARTPMQWDASEHAGFTTGKPWLEVGKSYRDINVETEKEGRIFPFY
                     QRLIALRKELPIIAEGDYRAAFKDSQAVYAFERHLGDQCLLVLNHFYAGEVELELPPR
                     YQHGQALISNYEKVSICEKVMLKPYQTLAILADN"
     gene            12208..14103
                     /gene="pepO"
                     /locus_tag="VC394_000187"
     CDS             12208..14103
                     /gene="pepO"
                     /locus_tag="VC394_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HEP5993688.1"
                     /translation="MTTYQDDFYQAVNGKWAETAVIPDDKPRTGGFSDLADEIEALML
                     DTTDAWLAGDHIPDDAILKNFVKFHRLVADYAKRDEVGVSPILPLIEEYQSLKSFSEF
                     VANIAKYELAGLPNEFPFSVAPDFMNAQLNVLWAEAPSILLPDTTYYEEGNEKAEELR
                     GIWRQSQEKLLPQFGFSTEEIKDLLDKVIELDKQLAKYVLSREEGSEYAKLYHPYVWA
                     DFKKLAPELPLDSIFEKILGQVPDKVIVPEERFWTEFAATYYSEANWDLLKANLIVDA
                     ANAYNAYLTDDIRVESGAYSRALSGTPQAMDKQKAAFYLAQGPFSQALGLWYAGQKFS
                     PEAKADVESKVARMIEVYKSRLETADWLAPATREKAITKLNVITPHIGYPEKLPETYA
                     KKVIDESLSLVENAQNLAKITIAHTWSKWNKPVDRSEWHMPAHLVNAYYDPQQNQIVF
                     PAAILQEPFYSLDQSSSANYGGIGAVIAHEISHAFDTNGASFDEHGSLNDWWTQEDYA
                     AFKERTDKIVAQFDGLESHGAKVNGKLTVSENVADLGGVACALEAAQSEEDFSARDFF
                     INFATIWRMKAREEYMQMLASIDVHAPGELRTNVTLTNFDAFHETFDIKEGDAMWRAP
                     KDRVIIW"
     gene            complement(14269..15309)
                     /locus_tag="VC394_000188"
     CDS             complement(14269..15309)
                     /locus_tag="VC394_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEP5993689.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDMDKAVELLREK
                     GMAKAAKKADRVAAEGLTGVYVHGNVAAVVEVNAETDFVAKNAQFVELVNATAKVIAE
                     GKPANNDEALALVMPSGETLAEAYVNATATIGEKISFRRFALIEKTDEQHFGAYQHNG
                     GRIGVISVVEGGDDALAKQVSMHIAAMKPTVLSYTELDAQFIKDELAQLNHAIELDNE
                     SRAMVDKPALPFLKYGSKAQLSDDVITAAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLDSVNAKAIAFARFEVGEGIEKKANDFES
                     EVAATMAAALNN"
     gene            complement(15443..16210)
                     /gene="rpsB"
                     /locus_tag="VC394_000189"
     CDS             complement(15443..16210)
                     /gene="rpsB"
                     /locus_tag="VC394_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEP5993690.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKLADQAYEFVRDAAANDAVILFVGTKKQAAEAVADEATRAGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEEGTFDVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNADPDDIDIIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDADVAFEADIQADSIEEIVEVVEGDNA"
     gene            16514..19588
                     /locus_tag="VC394_000190"
     CDS             16514..19588
                     /locus_tag="VC394_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEP5993691.1"
                     /translation="MTKGIRFQLLGSPHIFLDDKEQFFAFAKANALLYYLVVNGSVSR
                     EVAASLLWENKNTQTAKKNLRNAIYQVNKVLQADVIICPNRNLLVLKKTLDIKTDINL
                     FLADPLAHLELYQGEFLQGFYLKSGEEFDLWVSKMRMQYEQVYLKACYQKIEEKLSLD
                     AIEDVEEHLKQLIERDEFEEKNYQLLMRLYQQGNCPGKVIETYYQLANVLDKELGIQP
                     SLQSQQIYQEVVAKDRNERKIKHFLRNSNHFLGRIDEIKQLENYFANCLACQEVGALL
                     LIGDTGIGKRTLARQVLANQTQTFQIVTAKCFREEAMDSLLPWRNILDGLGDLVIQNR
                     LLTTKAWKAALKRCFPVATIFQEDNNQPFIKDHTSLLVSFIVDILQHLAEIKALVILI
                     EDCHWMDEDSLTLLQRVMNQLVHYPIAFVLTKHLGTTPELGLCLNALMSQGRLESICL
                     EPFNRQESLAYINSQLGSQPVTEEEMEHLYQASQGNPFFLSEYTQTLLRHEKFVPLTP
                     AIKAKLGLKLANLSSRDDALLNYLSCCRRPIPLNTLAQLMLLPLEEVIEMVDNLGHYY
                     ILVEESVGEEVLISFRQRIIQLYSYDRLSLSKRRLLHGQIAKRLEDLLPILTPSPHLL
                     DDIAYHYQESRQVIKALEYNLNYLDATLPFQHELFPIYSKSIGSLEKSDRDHQRLMEE
                     QFDRIRQSIADLELTYDNNRDFQQLLIRFSYLEGRYDIRTGRYQEGIKNIQKVIALAT
                     ELKQPSFLLEGYRQLIHYCIQVENKPEMRYYTSLSLEAAVAANHFEAIAISLRLNGLY
                     HLIIGELNEAERLLQQSIDFFKVTPGLQANYAIQIAAALDYLGEIAQIRYQFEKAVAY
                     QKQAIALTENKPAELSVSIFYIGLGISYFYLADFEQAEQILSLAKEALVNHSYPWKET
                     QLQIYLAMIQWKKGNYQPALTLLDYRETLMSRYRNPRDKGLVFYLMAVVKYQLIFQGA
                     TLSQQEKEMADYLLSESFEYYYEIASTNLNPYRDCHLVSELNDLRQQLSAKS"
     gene            complement(19619..20605)
                     /locus_tag="VC394_000191"
     CDS             complement(19619..20605)
                     /locus_tag="VC394_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HEP5993692.1"
                     /translation="MLEDYYPSTTSYYHGGIDDDLYTAKWGMVMTFLDLNDSSLTPFE
                     GTHFALIGFKSDKGVYINNGRVGAVESPAAIRTQLAKFPWHLGNQVMVYDVGNIDGPN
                     RSLEQLQNSLSKAIKRMCDLNLKPIVLGGGHETAYGHYLGLRQSLSPSDDLAVINMDA
                     HFDLRPYDQTGPNSGTGFRQMFDDAVADKRLFKYFVLGIQEHNNNLFLFDFVAKSKGI
                     QFLTGQDIYQMGHQKVCRAIDRFLEGQERVYLTIDMDCFSVGAAPGVSAIQSLGVDPN
                     LAVLVLQHIAASGKLVGFDVVEVSPPHDIDNHTANLAATFIFYLVQIMAQHN"
     gene            complement(20791..22332)
                     /gene="hutH"
                     /locus_tag="VC394_000192"
     CDS             complement(20791..22332)
                     /gene="hutH"
                     /locus_tag="VC394_000192"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEP5993693.1"
                     /translation="MTRVINLDGESLTIEDVIAIARQGVACRIDDSAIEAVNASRKIV
                     DDIVSEKRVVYGVTTGFGSLCNVSISPEDTVQLQENLIRTHASGFGDPLPEDAVRAIM
                     LIRINSLVKGYSGIRLSTIEKLLELLNKRVHPYIPEKGSLGASGDLAPLAHMVLPMLG
                     LGKAYYKGELLSGQEALDKAGIDKISLAAKEGLALINGTTVLTAIGALATYDAIQLLK
                     LSDLAGALSLEVHNGITSPFEENLHTIRPQSGQLATARNIRNLIEGSQNTTVATQSRV
                     QDPYTLRCMPQIHGASKDSIAYVKSKVDIEINSVTDNPIICKDGHVISGGNFHGEPMA
                     QPFDFLGIAISEIGNVSERRVERLVNSQLSKLPSFLVKYPGLNSGFMITQYACASLAS
                     ENKVLAHPASVDSIPSCENQEDFVSMGTTAARKAFEILKNSRRIVATEIMAACQALDL
                     KPENHELGKGTKVAYDLFRKEINFIEHDKHIEIYDELNKASAVIEDPSFLEAVEQAVE
                     LSIQF"
     gene            complement(22343..23686)
                     /locus_tag="VC394_000193"
     CDS             complement(22343..23686)
                     /locus_tag="VC394_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEP5993694.1"
                     /translation="MASNHMNEQEREQAKFSLSGATLYGINAVIGSGIFLLPRAIYKG
                     LGPASIAVMFGTAILTIMLAVCFAEVSGYFGKNGGAFQYSKRAFGDFIGFNVGFLGWT
                     VTIFAWAAMAAGFARMFIITFPAFEGWHIPLSIGLIILLSLMNIAGLKTSKIVTITAT
                     IAKLIPIVAFCACTLFFIKNGLPNFTPFVQLEPGTNLLGAISNTAVYIFYGFIGFETL
                     SIVAGEMRAPEKNVPRALLGSISIVSVLYMLIIGGTIAMLGSQIMMTNAPVQDAFVKM
                     IGPAGAWMVSIGALISITGLNMGESIMVPRYGAAIADEGLLPAAIAKQNQNGAPLVAI
                     LVSGAIAIVLLLTGSFENLAKLSVVFRFFQYIPTALAVMKLRKDDPDANVIFRVPFGP
                     IIPILAVIVSLVMIWGDNPMNFVYGAVGVIIASSVYYLMHGRNHQMDQMPPKEQI"
     gene            complement(23906..24502)
                     /locus_tag="VC394_000194"
     CDS             complement(23906..24502)
                     /locus_tag="VC394_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HEP5993695.1"
                     /translation="MTKVTIKAPSDYLQTDWSGGETNQLFLYPEDGDYKKREFSYRLS
                     KSTVALRQTTFTLLDGYHRILMTLDKPITLSDLSHAKEISLTPFEPYAFEGDTRIVSR
                     GICQDINLMYDDQYQGQLLPVWSDTPFEAPKADCQLLYALSKLTYSVTNQANQELEAN
                     HLLVIEQEASLSDLKVCPNQTSPAQTPVAVWAGLWYKT"
     gene            complement(24524..26197)
                     /locus_tag="VC394_000195"
     CDS             complement(24524..26197)
                     /locus_tag="VC394_000195"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEP5993696.1"
                     /translation="MVLSDIEIANSVTMEPISKVANQLGIDEEALCLYGKYKAKIDAR
                     QLVALKDKPDGKLILVTAISPTPAGEGKTTTSVGLVDALSAIGKKAVIALREPSLGPV
                     FGVKGGAAGGGHAQVVPMEDINLHFTGDFHAIGVANNLLAALIDNHIHHGNSLGIDSR
                     RITWKRVVDMNDRQLRHIVDGLQGKVNGVPREDGYDITVASEIMAILCLSENISDLKA
                     RLEKIIIGYNYQGEPVTAKDLKAGGALAALLKDAIHPNLVQTLEHTPALIHGGPFANI
                     AHGCNSVLATKLALKYGDYAVTEAGFGADLGAEKFIDIKCRMSGLRPAAVVLVATIRA
                     LKMHGGVPKANLATENVQAVVDGLPNLDKHLANIQDVYGLPVVVAINKFPLDTDAELQ
                     AVYDACDKRGVDVVISDVWANGGAGGRELAEKVVALAEQDNQFCFVYEEDDSIETKLT
                     KIVTKVYGGKGIRLTPAAKRELADLERLGFGNYPICMAKTQYSFSDDAKKLGAPTDFT
                     VTISNLKVSAGAGFIVALTGAIMTMPGLPKVPASETIDIDEEGNITGLF"
     gene            complement(26215..26841)
                     /locus_tag="VC394_000196"
     CDS             complement(26215..26841)
                     /locus_tag="VC394_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HEP5993697.1"
                     /translation="MGLVDLSLTDFAKVLGSDAPAPGGGSAAALSGANGISLTKMVCE
                     LTLGKKKYADYQAIITEIHAKSTALQASLLAAIDKDTEAFNLVSAVFDMPKETDEDKA
                     ARRTAMQKALKTAAQSPFEMMTLMVEALEITATAVGKSNTNAASDLGVAALNLKAGLQ
                     SAWLNVLINLSGIKDEDFVTDYRQKGQALLDKGCHLADDIYTKILDIV"
     gene            complement(26852..27751)
                     /gene="ftcD"
                     /locus_tag="VC394_000197"
     CDS             complement(26852..27751)
                     /gene="ftcD"
                     /locus_tag="VC394_000197"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003005485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HEP5993698.1"
                     /translation="MAKIVECIPNFSEGQNQAVIDGLVATAKSIPGVTLLDYSSDASH
                     NRSVFTLVGDDQSIQEAAFQLVKYASENIDMTKHHGEHPRMGATDVCPFVPIKDITTQ
                     ECVEISKQVAERINRELDIPIFLYEDSATRPERQNLAKIRKGQFEGMPEKLLEEDWAP
                     DYGDRKIHPTAGVTAVGARMPLVAFNVNLDTDNIDIAHKIAKIIRGSGGGYKYCKAIG
                     VMLEDRHIAQVSMNMVNFEKCSLYRTFETIKFEARRYGVNVIGSEVIGLAPAKALIDV
                     AEYYLQVEDFDYPKQVLENHLLG"
     gene            complement(27839..29869)
                     /locus_tag="VC394_000198"
     CDS             complement(27839..29869)
                     /locus_tag="VC394_000198"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEP5993699.1"
                     /translation="MSFYSETDIAAAMTVKLDDVLPEKTVFKEGIRRAPDRGFRLTQA
                     QTEIALKNALRYVPTKFHEEVIPEFLEELKTRGRIYGYRWRPKDRIYGKPINEYKGNC
                     TAAKAMQVMIDNNLSFEIALYPYELVTYGETGSVCANWMQYCLIKKYLEVMTDEQTLV
                     VESGHPVGLFQSKPEAPRVIITNGLLVGEYDNMKDWEIAEEMGVTNYGQMTAGGWMYI
                     GPQGIVHGTFNTLLNAGRLKLGVADDGDLTGKLFISSGLGGMSGAQGKAAEIAKAVAI
                     IAEVDQSRIETRHSQGWVTQIAESPEEALQLAQKAIDAKESTSIAYHGNIVDLLEYVN
                     DKQIHVDLLSDQTSCHNVYDGGYCPVGISFDERTRLLAEDKDTFHQMVDDTLARHFEA
                     IKTLTENGTYFFDYGNAFMKSVYDSGITEISKNGRNDKDGFIWPSYVEDIMGPMLFDY
                     GYGPFRWVCLSGKHEDLVATDKAAMEAIDPDRRYQDRDNYNWIRDAEKNQLVVGTQAR
                     ILYQDCIGRVTVALKFNELVRKGKIGPVMIGRDHHDVSGTDSPFRETSNIKDGSNVTC
                     DMAVQCYAGNAARGMSLVALHNGGGTGIGKAINGGFGLVLDGSERIDEIIKSAIAWDT
                     MGGVARRNWARNEHAIETAIEYNRLHAGTDHITIPYLADDDLVTSAVDQLFH"
     gene            30161..31426
                     /locus_tag="VC394_000199"
     CDS             30161..31426
                     /locus_tag="VC394_000199"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEP5993700.1"
                     /translation="MVADVLLTHFNQLFCLNDPGHPLTGQEMKKATIVEDGYIAIKDG
                     LIVALGSGEPDAELVGPQTIMRSYKGKIATPGIIDCHTHLVYGGSREHEFAKKLAGVS
                     YLDILAQGGGILSTVRATRSASFDNLYQKSKRLLDYMLLHGVTTVEAKSGYGLDWETE
                     KRQLDVVAALEKDHPIDLVSTFMAAHAIPEEYKGNPKAYLDVIIKDMLPVVKEENLAE
                     FCDIFCEKNVFTADESRYLLSKAKEMGFKLRIHADEIASIGGVDVAAELSAVSAEHLM
                     MITDDGIAKLIGAGVIGNLLPATTFSLMEDTYAPARKMIDAGMAITLSTDSNPGSCPT
                     ANMQFVMQLGCFMLRLTPIEVLNAVTINAAYSVNRQERVGSLTVGKEADIAIFDAPNI
                     DYPFYFFATNLIHQVYKKGQLTVDRGRIL"
     gene            complement(31484..33016)
                     /gene="ahpF"
                     /locus_tag="VC394_000200"
     CDS             complement(31484..33016)
                     /gene="ahpF"
                     /locus_tag="VC394_000200"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEP5993701.1"
                     /translation="MALSPDIKEQLAQYLTLLEADLVLQVSLGDNEQSQKVKDFVEEI
                     AAMSERISIENITLDRQPSFKVAKKGHGSGVVFAGLPLGHELTSFILALLQVSGRAPK
                     VDQDVIDRIKAIDRPLHFETYVSLTCHNCPDVVQALNIMSVLNDKISHTMVEGGMFQD
                     EVKAKGIMSVPTVFLDGEEFTSGRATIEQLLEQIAGPLSEEAFADKGLYDVLVIGGGP
                     AGNSAAIYAARKGLKTGLLAETFGGQVMETVGIENMIGTLYTEGPKLMAEVEAHTKSY
                     DVDIIKAQLATSIEKKENIEVTLANGAVLQAKTAILALGAKWRNINVPGEDEFRNKGV
                     TYCPHCDGPLFEGKDVAVIGGGNSGLEAALDLAGLAKHVYVLEFLPELKADKVLQDRA
                     AKTNNMTIIKNVATKDIVGEDHVTGLSYTERDSGEDKHLDLEGVFVQIGLVPNTAWLK
                     DSGVNLTDRGEIIVDKHGSTNIPGIFAAGDCTDSAYKQIIISMGSGATAAIGAFDYLI
                     RQ"
     gene            complement(33037..33597)
                     /gene="ahpC"
                     /locus_tag="VC394_000201"
     CDS             complement(33037..33597)
                     /gene="ahpC"
                     /locus_tag="VC394_000201"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002273221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEP5993702.1"
                     /translation="MSLIGKEIAEFSAQAYHDGKFITVTNEDVKGKWAVFCFYPADFS
                     FVCPTELGDLQEQYETLKSLGVEVYSVSTDTHFVHKAWHDDSDVVGTITYPMIGDPSH
                     LISQAFEVLGEDGLAQRGTFIVDPDGIIQMMEINADGIGRDASTLIDKIHAAQYVRKH
                     PGEVCPAKWKEGAETLTPSLDLVGKI"
     gene            34004..34074
                     /locus_tag="VC394_000202"
     tRNA            34004..34074
                     /locus_tag="VC394_000202"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34036..34038,aa:Cys,seq:gca)
     gene            34593..34796
                     /locus_tag="VC394_000203"
     CDS             34593..34796
                     /locus_tag="VC394_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEP5993703.1"
                     /translation="MAQGTVKWFNAEKGFGFISTENGQDVFAHFSAIQTNGFKTLEEG
                     QKVAFDVEEGQRGPQAVNITKLA"
     gene            34992..35453
                     /locus_tag="VC394_000204"
     CDS             34992..35453
                     /locus_tag="VC394_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEP5993704.1"
                     /translation="MPTKNTSDSIEEYIKELLAKSGIAEIKRSMLADSFQVVPSQINY
                     VIKTRFTESRGYEVESKRGGGGYIRIAKVHFSDKHHLIGNLMATIEDCISEQVFTDSI
                     QLLFDEHLLTEREGNIILAVASDDVLGTDGSTIRARMLYRLLQRIDRKGSN"
     gene            35453..37897
                     /locus_tag="VC394_000205"
     CDS             35453..37897
                     /locus_tag="VC394_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEP5993705.1"
                     /translation="MIMYSTKMQDIFRQAQFQAARFDSHCLETWHVLLAMVAVDNSLA
                     NMILSEYDAQVAIEEYEAAAILAMGKTPKEQLSRVDFRPQSKTLTNLLAFAQAISQIT
                     RDQEVGSEHVLFAILLNPDIMASRLLEIAGYQIKDNGNGQPRLADLRKAIERHAGYSK
                     EMIKAIHELRKPKKTKTQGTFSDMMKPPSTAGELSDFTRDLTEMARQGLLESVIGRDQ
                     EVSRMIQVLSRKTKNNPVLVGDAGVGKTALAYGLAQRIANGAIPYELKEMRVLELDMM
                     SVVAGTRFRGDFEERMNQIIDDIEADGQIILFVDELHTIMGSGSGIDSTLDAANILKP
                     ALSRGTLHMVGATTQEEYQKHIEKDAALSRRFAKILIEEPNTEDAYQILMGLKLSYET
                     YHNVSISNEAVKTAVKMAHRYLTSKNLPDSAIDLLDEASAAVQNMVKKSAPETLTPID
                     QALINGDMKKVSRLLAKEAKGQMRKPTPVTEDDILATLSKLSGIPLEKLTQADSKKYL
                     NLEKELHKRVIGQDAAVTAISRAIRRNQSGIRTGKRPIGSFMFLGPTGVGKTELAKAL
                     AEVLFDDEAALIRFDMSEYMEKFAASRLNGAPPGYVGYDEGGELTQKVRNKPYSVLLF
                     DEVEKAHPDIFNVLLQVLDDGILTDSRGRKVDFSNTIIIMTSNLGATALRDDKTVGFG
                     VKGIHQDHQAMEKRILEELRKTYRPEFINRIDEKVVFHSLTQDNMRDVVKIMVQPLIT
                     TLAEKGITLKIQPLALKHLSEVGYDEHMGARPLRRTLQTEIEDKLSELILSRELTSGH
                     TLKIGLSYGKLTFHIA"
     gene            38075..38365
                     /gene="groES"
                     /locus_tag="VC394_000206"
     CDS             38075..38365
                     /gene="groES"
                     /locus_tag="VC394_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEP5993706.1"
                     /translation="MLKPLGDRVVVRFDDEKEQTVGGFVLAGTHKESTRKATVLAVSE
                     TGVRTITGDSVLPSVSVGQEVLVENGHDLEVTVDDEKVSIIRESDIIAIVTK"
     gene            38401..40032
                     /gene="groL"
                     /locus_tag="VC394_000207"
     CDS             38401..40032
                     /gene="groL"
                     /locus_tag="VC394_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880885.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEP5993707.1"
                     /translation="MAKDIKFSADARAAMVRGVDMLADTVKVTLGPKGRNVVLEKAFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVHEG
                     LKNVTAGANPIGIRRGIETATATAVEALKAIAQPVSGKEAIAQVAAVSSRSEKVGEYI
                     SEAMERVGNDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDNEKMVADLENPFI
                     LITDKKVSNIQDILPLLEEVLKTNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATMTALGQAAKITVDKDSTVIVEGS
                     GSSEAIANRIALIKSQLETTTSDFDREKLQERLAKLAGGVAVIKVGAPTETALKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALITVIEKVAALELEGDDATGRNIVLRALEEPVRQ
                     IALNAGYEGSVVIDKLKNSPAGTGFNAATGEWVDMIKTGIIDPVKVTRSALQNAASVA
                     SLILTTEAVVANKPEPAAPAPAMPAGMDPGMMGGF"
     gene            40283..40549
                     /locus_tag="VC394_000208"
     CDS             40283..40549
                     /locus_tag="VC394_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP5993708.1"
                     /translation="MNHFGEIFKTFRESKGLRLKDVAKAGISTSQLSRFEKGETDLTI
                     STFMLILDESNMSIDEFMYAVHDFHRDDLNELLSKSEGFRNNSR"
     gene            complement(41272..42771)
                     /locus_tag="VC394_000209"
     CDS             complement(41272..42771)
                     /locus_tag="VC394_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HEP5993709.1"
                     /translation="MINKKISLGVLSILTAFSLQSVSYACTGFIIGKDLTKDGSLLYG
                     RTEDLEPHHNKNFIVRLAKDNPAGEKWKDPSNGFEYPLPEHSYRYSAIPDVTPNKGVY
                     DEAGFNEFGVSMSATVSASANDAIQKIDPYVKNGLAESSMASVILPSVKTAREGVALI
                     AKIVTEKGAAEGNIVTLADKDGIWYMEILSGHQYVAIKFPDDKYAVFPNTFYLGHVDF
                     NDKENTIASEDVEKVTKKAKSYTEVDGKFHIAKSYNPPLNDANRSRSFSGIKSLDPDS
                     KVTYKDSNYELLQSTDKTFSLEDAMKLQRNRFEGLDLKPLDQMALDGKGKPKSKKAVK
                     GYAYPISNPNVMEAHIFQLKKDIPAELGGGVMWLSIGSPRNAPYLPYLGNISRTYEAY
                     QEKSTQYNDKSWYWTVSHINDLVAAHPKPFGTKVIDEMKGLEKTWIAEQDKSTKEISD
                     LVVSDPKAAQEKADKISLDRAEKTFKRLKAIEAKLVEEKPQNKKGLNRS"
     gene            43018..44658
                     /locus_tag="VC394_000210"
     CDS             43018..44658
                     /locus_tag="VC394_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5993710.1"
                     /translation="MTKPFHHKKLKQITIIAATSLFLFLIGGAFYYSKNHCINAYLKA
                     RSAQSGPVFENIKAYLVWDDTNEQITNDEAMYTKFRRYSQKELRQKKQDLKAASQDSA
                     VQVKSVGRRFWIFPDYRIAIKPMDLTIKTNVPQADVLLNQKKVAVSDSEQFSVKLDRL
                     PTAEYTASIRGKHNGRNIKVNKSYDGDNPVLDLSVSFRTFLVTSNAKQGDLYFDDNHI
                     GTLKDGQLQVEDYPVTENAQAYMKTTFPDGELRSQKYALADVEEGATLEILVTDLLEE
                     DKAGELLVSAFDQLMHYLSTGQDSSNLRSVFEAGSSNAFYRGLKESIKAKFQTDTRKA
                     SRLNIPSILLTTMTQVGKTTYVLDFTATYEFLYDNSTDPEQHTSGHINQDLTGKVTVK
                     KVGQHYLISQSGSKNITVVKEDNQLKAPSVFPESILGTWTGQANGLSIHMSLASDGTI
                     TTKVEDRKGNRSKETRTAKISKVEDKGNGFYLYTPDPGSDISALAPEGGLGGANVKYA
                     YGFKISGKTASPVVWQAALTHEFDYTKPLSGVTLQKQP"
     gene            complement(44747..45748)
                     /locus_tag="VC394_000211"
     CDS             complement(44747..45748)
                     /locus_tag="VC394_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEP5993711.1"
                     /translation="MTSVKVLKLSLLLLPPKWLSTLLKNTITGSMKTTNKPSKGFTVT
                     LKNPFEELTVKALLEEQLLIPRKIRHFLRTKKHVLINGHPVNWQSCVKYGDQVKLFFD
                     HEDYPEKIIVMGQAEKVTCLYEDEHIIIVNKPEGMKTHGNDPTELALLNHVSAYTGQT
                     CYVVHRLDKETSGAILFAKTPFILPILNRLLEKRDIHREYLALVHGSLDSPRVTYHHP
                     IGRHRHDRRKRVVDPINGKKAITEVTLVKNFHKTASLLNCQLQTGRTHQIRVHLAHQG
                     HVLFGDPLYSNGKKDCARLMLHAYQLRLKHPLTQEDICVQAKSATFDAVLNAQKARK"
     gene            46325..46705
                     /locus_tag="VC394_000212"
     CDS             46325..46705
                     /locus_tag="VC394_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEP5993712.1"
                     /translation="MKTIRRYDVNEDRGHTGLVEAGDFYYLNYCVGNVGQDIESQING
                     AFDEMERRLALVGLTLDAVVQMDCLFRDVWNIPVMEKMIKERFNGRYPARKSIQTEFA
                     HHGGPQGLLFQVDGVAYSKHISMT"
     gene            46744..49080
                     /locus_tag="VC394_000213"
     CDS             46744..49080
                     /locus_tag="VC394_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEP5993713.1"
                     /translation="MRFLELLQKKFFPKAYQEKQFLMHQKTRLTPQHNQKQYSPNANH
                     LDSSATKNSEQDPAIALQRSRAYEGSPKSRPAWLQKLEAVLPSPQHPIRRFWRRYHIG
                     KLLMILIGTLVLLLGSYLFYLSKTAKVSDLQDALKATTVIYDHKGEYAGSLSGQKGSY
                     VELNAISDDLENAVIATEDRTFYSNSGINLKRFLLAVVTAGRFGGGSTITQQLAKNAY
                     LSQDQTIKRKAREFFLALELTKKYSKKDILTMYLNNSYFGNGVWGVEDASQKYFGTTA
                     ANLTLDEAATLAGMLKGPEIYNPYHSLKNATHRRDTVLGAMVDAKKITQTKAQQARAV
                     GLKNRLADTYVGKTDDYKYPSYFDAVISEAIATYGLSEKDIVNNGYKVYTELDQNYQT
                     GMQTTFNNDELFPVSAYDGSSAQAASVALDPKTGGVRGLIGRVNSSENPTFRSFNYAT
                     QAKRSPASTIKPLVVYAPAVASGWSIEKELPNTVQDFDGYQPHNYGNYESEDVPMYQA
                     LANSYNIPAVSTLNDIGIDKAFTYGKTFGLDMSSAKKELGVALGGSVTTNPLEMAQAY
                     AAFANNGVIHPAHLINRIENARGEVLKTFTDKAKRVVSQSVADKMTAMMLGTFSNGTA
                     VNANVYGYTLAGKTGTTETNFNPDLAGDQWVIGYTPDVVISQWVGFNQTDENHYLTDS
                     SAGTASAIFSTQASYILPYTKGSQFHVDNAYAQNGISAVYGVNETGNQSGVDTQSIID
                     GLRKSAQEASQSLSKAVDQSGLRDKAQSIWKEIVDYFR"
     gene            49129..49281
                     /gene="rpmG"
                     /locus_tag="VC394_000214"
     CDS             49129..49281
                     /gene="rpmG"
                     /locus_tag="VC394_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEP5993714.1"
                     /translation="MAQKKASLACVECGSRNYSIGVSSTPKPTRLEVNKFCKYCKTYT
                     LHKETR"
     gene            49295..49471
                     /gene="secE"
                     /locus_tag="VC394_000215"
     CDS             49295..49471
                     /gene="secE"
                     /locus_tag="VC394_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEP5993715.1"
                     /translation="MGFISGTFKVLKDTTWPNRKQRWKDFISVLEYTAFFTVIIYIFD
                     QLLAKSVLALINLF"
     gene            complement(49708..50481)
                     /locus_tag="VC394_000216"
     CDS             complement(49708..50481)
                     /locus_tag="VC394_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEP5993716.1"
                     /translation="MTDRGIVFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCANPESQQ
                     KAPEQMLTSDGLNTKIVGEEKTVDEVIEEVLKDLDFYEESGGGMTLSGGEIFAQFDFA
                     LALLKAAKAAGLHTAIETTAFAKHEQFVTLIDYVDFIYTDLKHYNQLRHQKVTGVRND
                     LIIKNIHYAFQAGKEIVLRIPVIPQFNDSLDDAKAFSELFNQLEIDQVQLLPFHQFGE
                     NKYKLLGREYEMAEVKAYHPEDLADYQAVFLNHNIHCYF"
     gene            50607..51353
                     /locus_tag="VC394_000217"
     CDS             50607..51353
                     /locus_tag="VC394_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEP5993717.1"
                     /translation="MNRLERIIQLVSQKKKIDVNSLSEQLEVSKVTIRKDLDKLESKG
                     LLRREHGYAVLNSGDDLNVRLSYNYNIKRRIAEKAAELVQDNDTIMIESGSTCALLAE
                     VLCQTKRNIKIITNSCFIANYIRQYSSCQIILLGGYYQPNSEVTVGPLLKEMISLFHV
                     NRVFVGTDGFNKDLGFMGKDMMRSEGVRYMADAAEEVVILTDSSKFSKTSLVHQLSLA
                     DVNRVITDQALDKQTQELLSSGGLVLDFVS"
     gene            51369..52349
                     /locus_tag="VC394_000218"
     CDS             51369..52349
                     /locus_tag="VC394_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEP5993718.1"
                     /translation="MKEERRRLLAKVAYLHYVQGKSQTLISKEMNIYRTTVCRMLAKA
                     KEEGIVRIEIADYDADLFALEEYVRKQYGLEKLDLVPNQVEDTPMDTLTNVAKTAAEV
                     FRHVVKDGDKIGLSWGATLSCLMDELNPKAMKDVFIYPLAGGPSHINAKYHVNTLVYR
                     LARIFHGNSAFMNAMVIQEDKHLAKGILQSKYFNDILTSWDQLDLALVGIGGEPNSLE
                     QSQWRDLLTSSDHDQLKYEKAVGEVCCRFFDQAGQPVYTGLQDRTIGISLEQLRRVPK
                     TMAVATGKHKAKAILAALKAGFINYLVTDKETMLAVLALDEDIDLNNVLL"
     gene            52637..52957
                     /locus_tag="VC394_000219"
     CDS             52637..52957
                     /locus_tag="VC394_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEP5993719.1"
                     /translation="MQVIVPDQIIMGLILNAGDAKQHIYQALKCAKEDDYATSEKEMA
                     LADDALLEAHNLQTQFLAQEASGNKSEITALFVHSQDHLMTTITEINLIKEIIDLRKE
                     LATK"
     gene            52985..53293
                     /locus_tag="VC394_000220"
     CDS             52985..53293
                     /locus_tag="VC394_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEP5993720.1"
                     /translation="MIKIGLFCAAGFSTGMLVNNMKVAAEKKGIDCQIEAYAQGKLAD
                     YAPLLDVALLGPQVAYTLDKSEAICKDNDIPIAVIPMADYGMLDGNKVLDLALSLVKE
                     "
     gene            53303..54607
                     /locus_tag="VC394_000221"
     CDS             53303..54607
                     /locus_tag="VC394_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEP5993721.1"
                     /translation="MAKMNMQNIIKPIMTFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     AGQIPFQGVNDAIASVFGADWTEPFMQVYHGTFAIMGLISCFAIGYSYAKNSDVEPLP
                     SGVLSLSAFFILLRSSYVPAEGEAIGDAISKVWFGGQGIIGAIVIGLTVGAVYTTFIR
                     RHIVIKMPDQVPQAIAKQFEAMIPAFVIFTLSMLVYIIAKSVTGGGTFIEMIYDVIQV
                     PLQGLTGSLYGALGIAFFISFLWWFGVHGQSVVNGIVTALLLSNLDANKALMAAGELS
                     LDKGAHIVTQQFLDSFLILSGSGITFGLVVAMIFAAKSKQYKALGKVAAFPALFNVNE
                     PVVFGFPIVMNPVMFLPFILVPVLAALTVYGAIAIGFMQPFAGVTLPWSTPAIISGFM
                     VGGWQGAIVQILILIMSTLVYFPFFKIQDNMAYQNEQASEES"
     gene            54818..57235
                     /locus_tag="VC394_000222"
     CDS             54818..57235
                     /locus_tag="VC394_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEP5993722.1"
                     /translation="MIETKSPYFGHLTDRMAHYREAVLDKKPYIDAERAILATEAYQK
                     HQNKPANLKRAYMLQTILENMTIYIEDESLIAGNQASSNKDAPIFPEYTLEFVLNELD
                     LFEKRDGDVFYITEETKQQLRDIAPFWENNNLRARCGVLLPEEVQVYMETGFFGMEGK
                     MNSGDAHLAVNYQKLLEHGLKGFEERARAAKAALDLTIPENIDKYHFYDSVFIVIDAV
                     KTYAKRYAKLARELAKTAKPERQAELLDIARICDKVPYEPAKTFAEAVQSVWFIQCIL
                     QIESNGHSLSYGRFDQYMYPYVKADLEAGRETEDTIVERLTNLWIKTLTINKVRSQAH
                     TFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLRSVAQTKLPQPNLTVRYHKGLDNT
                     FMNECIEVMKLGFGMPAMNNDEIIIPSFIKKGVSEEDAYDYSAIGCVETAVPGKWGYR
                     CTGMSYINFPKILLITMNDGIDPASGKRFAKGHGHFKDMTSYEELKAAWDATLREITR
                     MSVIVENAIDLGLEREVPDILCSALTDDCIGRGKTLKEGGAVYDYISGLQVGIANLSD
                     SLAALKKLVFEEGRLTPEELWHALESDFAGERGEEIRQMLINDAPKYGNDDDYADSLV
                     VEAYDTYIDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGKGTLATPDGRHAGTPL
                     AEGCSPEHSMDKKGPTSVLKSVAKLPTDEIVGGVLLNQKVNPQTLAKEEDKLKLMALL
                     RTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSKATQDDII
                     ERTEHTL"
     gene            57248..57916
                     /locus_tag="VC394_000223"
     CDS             57248..57916
                     /locus_tag="VC394_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEP5993723.1"
                     /translation="MEYMLDTLDLEAIKKWHHILPLAGVTSNPSIAKKEGDIDFFERI
                     REVRAIIGDKASIHVQVIAQDYEGILKDAAEIRRQCGDSVYVKVPVTTEGLAAIKTLK
                     AEGYHITATAIYTTFQGLLAIEAGADYLAPYYNRMENLNIDPEAVIGQLAEAINRENA
                     DSKILAASFKNVAQVNKSFALGAQAITAGPDVFEAGFAMPSIQKAVDDFGKDWEAIHH
                     RKSI"
     gene            57973..59061
                     /locus_tag="VC394_000224"
     CDS             57973..59061
                     /locus_tag="VC394_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEP5993724.1"
                     /translation="MKVFASPSRYIQGKNALFTNAKTLKQLGNSPILLCDDVVYGIVG
                     ERFESYLIDNGMTPVHVAFNGEASDNEISRVVAIAKENGNDVIIGLGGGKTIDSAKAI
                     ADLLAVPVIIAPTIASTDAPTSALSVIYTDEGAFEKYIFYSKNPDLVLVDTQVICQAP
                     KRLLASGIADGLATWVEARAVMQKNGDTMAGGNQTLAGVAIAKACEQTLFADGLKAMA
                     SCDRQVVTPALENVIEANTLLSGLGFESAGLAAAHAIHNGFTALTGAIHHLTHGEKVA
                     YGTLTQLFLENRSREEIDRYIDFYQAIGMPTTLKEMHLDTATQEDFLKIGRQATMAGE
                     TIHQMPFVISPEDVAAALVAVDAYVTSR"
     gene            complement(59143..59652)
                     /locus_tag="VC394_000225"
     CDS             complement(59143..59652)
                     /locus_tag="VC394_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEP5993725.1"
                     /translation="MSTDHHLREVSYQLLAERGVSLEAIAELVLFLQNDYIPNLTMAE
                     CLESVEAVLAKREVQNAIITGVELDKLAEANQLSEPLLSILKTDQGLYGIDEILALSI
                     VNLYGSIGFTNYGYLDKTKPGIVDKLNHKDGNSCHTFLDDIVSAIAAAAASRIAHNDP
                     AKSAITNQQ"
     gene            60016..60831
                     /gene="sdaB"
                     /locus_tag="VC394_000226"
     CDS             60016..60831
                     /gene="sdaB"
                     /locus_tag="VC394_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptodornase B"
                     /protein_id="HEP5993726.1"
                     /translation="MNLLGSRRVFSKKCRLVKFSMVALVSATMAVTTVTLENTALARQ
                     TQVSNDVVLNDGASKYLNEALAWTFNDSPNYYKTLGTSQITPALFPKAGDILYSKLDE
                     LGRTRTARGTLTYANVEGSYGVRQSFGKNQNPAGWTGNPNHVKYKIEWLNGLSYVGDF
                     WNRSHLIADSLGGDALRVNAVTGTRTQNVGGRDQKGGMRYTEQRAQEWLEANRDGYLY
                     YEAAPIYNADELIPRAVVVSMQSSDNTINEKVLVYNTANGYTINYHNGTPTQK"
     gene            complement(61072..61914)
                     /locus_tag="VC394_000227"
     CDS             complement(61072..61914)
                     /locus_tag="VC394_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEP5993727.1"
                     /translation="MEIGETVEFIRHSKNISIKQVCGDYLTRQTYYRFIKNNLDISSK
                     KLLYILDNLNVNVDEFLFISNNFKQYKEFIDMDTAKHYFECRNIEGLNHILDSYKDSK
                     STKEKNLFALVKVLLATLTEEDCLTERTYLSNYLINIETWSHYETVLFNNCMFIFESC
                     FIEMVFSKVILNLDKYNTLRYYGNESIRMFVNMLILFIQRQEYDKASEILAKIEDYQL
                     NDDCLYERCCVSFFDGIIGLINGKEGAEQKCVQILEIFQLLNCKTIHHMFQTYLEAIK
                     HKLS"
     gene            62855..64051
                     /gene="speB"
                     /locus_tag="VC394_000228"
     CDS             62855..64051
                     /gene="speB"
                     /locus_tag="VC394_000228"
                     /EC_number="3.4.22.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine proteinase exotoxin SpeB"
                     /protein_id="HEP5993728.1"
                     /translation="MNKKKLGIRLLSLLALGGFVLANPVFADQNFARNEKEAKDSAIT
                     FIQKSAAIKAGARSAEDIKLDKVNLGGELSGSNMYVYNISTGGFVIVSGDKRSPEILG
                     YSTSGSFDANGKENIASFMESYVEQIKENKKLDTTYAGTAEIKQPVVKSLLDSKGIHY
                     NQGNPYNLLTPVIEKVKPGEQSFVGQHAATGCVATATAQIMKYHNYPNKGLKDYTYTL
                     SSNNPYFNHPKNLFAAISTRQYNWNNILPTYSGRESNVQKMAISELMADVGISVDMDY
                     GPSSGSAGSSRVQRALKENFGYNQSVHQINRSDFSKQDWESQIDKELSQNQPVYYQGV
                     GKVGGHAFVIDGADGRNFYHVNWGWGGVSDGFFRLDALNPSALGTGGGAGGFNGYQSA
                     VVGIKP"
     gene            64053..64358
                     /locus_tag="VC394_000229"
     CDS             64053..64358
                     /locus_tag="VC394_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spi family protease inhibitor"
                     /protein_id="HEP5993729.1"
                     /translation="MEMHFVRTEPEARRIAETFCAENTQTKTPMRVQQLSYPSDTDHS
                     GGELYIYALSPAGFIIVSGDTRAHTILGYSFDNNLDLNHDNVRSMIEAYQTQINSLD"
     gene            64836..65765
                     /gene="prsA"
                     /locus_tag="VC394_000230"
     CDS             64836..65765
                     /gene="prsA"
                     /locus_tag="VC394_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEP5993730.1"
                     /translation="MKQMNKLITGVVTLATVVTLSACQSSHNNTKLVSMKGDTITVSD
                     FYNETKNTELAQKAMLSLVISRVFETQYANKVSDKEVEKAYKQTADQYGTSFKTVLAQ
                     SGLTPETYKKQIRLTKLVEYAVKEQAKNETISKKDYRQAYDAYTPTMTAEIMQFEKEE
                     DAKAALEAVKAEGADFAAIAKEKTIAADKKTTYTFDSGETTLPAEVVRAASGLKEGNR
                     SEIITALDPATSKRTYHIIKVTKKATKKADWKAYQKRLKDIIVTGKLKDPDFQNKVIA
                     KALDKANVKIKDKAFANILAQFAKPNQKQPAQK"
     gene            complement(66041..66277)
                     /locus_tag="VC394_000231"
     CDS             complement(66041..66277)
                     /locus_tag="VC394_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5993731.1"
                     /translation="MTHRFFLTPLSTLWRRLRPKRYSKKVLADEPTSYQPPAAHGPCD
                     DIHQLLEDKSLSDDYLIATSAMRHLWYWGYSQIG"
     gene            66701..69781
                     /locus_tag="VC394_000232"
     CDS             66701..69781
                     /locus_tag="VC394_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin binding protein"
                     /protein_id="HEP5993732.1"
                     /translation="MTNCKYKLRKLSVGLVSVGTMLIAPTVLGQEVSASSSTESSTTT
                     ANTGTGTASGMTATTPSATTDTGEAAGSGARSEANGASSVVSSEESQSSGTTPASPQA
                     QTAPAATSTSSASSSNEKTPKTATTTTSSTPVASTSNNSNKVTSTEAETQTMDVERYT
                     VDKENSKLNIKDGKTPKTRSSVNKDTKLIRNRDDKQRDIVDVTRTVKTNEDGTIDVTV
                     TVKPKQIDEGADVMALLDVSQKMTKENFDKAKEQIKKMVTTLTGEPTDGKENHNRRNS
                     VRLMTFYRKVNEPIELTAENVDKTLDEVWKKAKEDWDWGVDLQGAIHKAREIFNKEKE
                     KKSGKRQHIVLFSQGESTFSYDIKNKSDITKTKITEKVTTSSPLFPWLPIFNHTNRKA
                     EIIGDLEKVLDMAEKVGISLPSSLKSAVKALGLTNSAIGSILGKGLTEYLGLTEYSSD
                     NLDGGGFDYSKRVGEGYYYHSFSDRKYENTMPLEEAIRTALASNFSKLTDNWFFDILN
                     SFVNKDTVEKAKLDVIMKVLNSIFYKREYRYYNHNLSAIAEAKMAQQEGITFYSVDVT
                     DLNSASKRVRRQAAVRKGTKEEENKKNEEERNTKFDTYLKKMSEGNNFLSNVEERDKF
                     KDTLTELTIKDEFTDKVTVEKDSWSKSITDGLKNSNNNNVKHQQANTSTWSFFSSSKE
                     SLTWIISKEQLKEAFEKNGSLTFKYKLRVNKDKLLDKDKKRTKRDTPTENKTSVTADI
                     ISNTVDYKINNQEVKGNKLDAVKLTYTKETVPVPDVEGEVVPIPEKPLVEPMTPLYPA
                     IPNYPTPQLPKDEDLEISGGHGPIVDIVEDTGTGVEGGAQNGVVSTQENKDPIVDITE
                     DTQPGMSGSNDATVVEEDTAPKRPDVLVGGQSDPIDITEDTQPSVSGSNDATVVEEDT
                     VPKRPDILVGGQSDPIDITEDTQPGMSGSNDATVIEEDTKPKRFFHFDNEPQAPEKPK
                     EQPSLSLPQAPVYKAAHHLPASGDKREASFTIVALTIIGAAGLLSKKRRDTEEN"
     gene            69961..71898
                     /locus_tag="VC394_000233"
     CDS             69961..71898
                     /locus_tag="VC394_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin binding protein"
                     /protein_id="HEP5993733.1"
                     /translation="MTKKFIKSAVILGLASITISMTQPVEAISNSLEYQAQYERVKEE
                     LDALKEKGPKLGERSLYFRLVNKFLSWFGQVWDPLAIKETDYLLDLIGQVNYLVSNTE
                     FQAPEYKDAKNKLDLAVAGYDLYLAREKKRGDAPDYQVTQLTLEQLKTIKKKIREALE
                     TFAQEVKTIQQKNPDLFPHAYVLNAKSKDITDKAQPFLVNSYYDVFYKQFLFTNTNDG
                     YNYNKEADNLTSYQNLIKELLGLKAEIDQLTKGKTGDDRLEMLMWQSKFTLSDSRKKS
                     HLSAYYTQGEYVDKVVLSEEAPNLRTAKEKVKTLKGILHDYYVETNKPEKAKKYHVED
                     ETLPSQPEAPATPEAPAPAPVPVPGQKPAEDERSPQATEPANPSKEDSSTDASQGSHD
                     SENPATDSPSQPQAPDQGNHQSQVPNAKPQTDKTDTPNAPAIPQDTPKAPEAGGQSGP
                     AGNTEEKAPDSGPKESDQSSSKESPSVGEDTTPNQPDVLVGGQSEPIDITEDTITDAP
                     PTVSGHNASTQPQSVVEDTAPQRPDVLVGGQSEPIDITQDTQPGMSGSNDATVINEDT
                     KPKRVFHFDNKEPQASEKAAEQKLAPHDSHTTPQASDDTAAPHLPASGDKPEKVFALA
                     ALTMVAAAGLLSKKRRNTDKN"
     gene            72040..72336
                     /locus_tag="VC394_000234"
     CDS             72040..72336
                     /locus_tag="VC394_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HEP5993734.1"
                     /translation="MTKQDQLIVEKMEQTYEAFSPKLANLIEALDAFKEHYEEYATLR
                     NFYSSDEWFRLANQPWDDIPCGVLSEDLLFDMIGDHNQLLADVLDLAPIMYKHM"
     gene            72485..72769
                     /locus_tag="VC394_000235"
     CDS             72485..72769
                     /locus_tag="VC394_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEP5993735.1"
                     /translation="MKFKKVLVIPALALAATCFLTACGTKKDSKKEEVKEIKMSDIKD
                     DAVSKKTKVVDGEEVTEYTTKDGNVIQIPAGNEEGMESKDAGGSGAPAKN"
     gene            73203..74471
                     /locus_tag="VC394_000236"
     CDS             73203..74471
                     /locus_tag="VC394_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEP5993736.1"
                     /translation="MFQLRKKMTRKQLALLSAGVLTCVVGGSYLIMNHQQQEIVSSVN
                     KVKALTIKEAMEQGKDISLTLAGEVTANNSSKVKIDSSKGEVKDVFVKKGDVVKVGQP
                     LFSYETSQRLTAQSSEFDVQTKANQLQVAKTNAALKWETYNRKVNEINTLKSRYNTAP
                     DESLLEQIRSAEDSVSQALSDAKTADSDVKTAQIELDKANATATTEKGKLEYDTVKSD
                     TAGTIVSLNTDLPNQSKSKKENETFMEIIDKSKMLVKGNISEFDRDKLKIGQKVEVID
                     RKDNSKKWTGKVTQVGNLKAEEKGQGQGQGGNDQQDNPNQAKFPYVIELDQSDKQPLI
                     GSHTYVNVLNNVPEAGKIVLKETFTMAENGKTYVWKVDKNKVKKQEIKTKPFSKGYVE
                     VTSGLTMQDKIAQPLPGMKDGMEVGSIVKP"
     gene            74458..75132
                     /locus_tag="VC394_000237"
     CDS             74458..75132
                     /locus_tag="VC394_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5993737.1"
                     /translation="MLNLKDIRKSYHLGTEEFAILKGIDLEVNEGDFLAIMGPSGSGK
                     STLMNIIGCLDKPGSGSYAIEGRDVSSLSDNELADLRNQKIGFVFQNFNLMPKLTACQ
                     NVELPLTYMNVPKKERRKRALEMLKLVGLEERSEFKPMELSGGQKQRVAIARALVTNP
                     SFILGDEPTGALDTKTSVQIMDLFKQFNDNGKTIIIITHEPEVAALCKKTVILRDGNI
                     EHSDIE"
     gene            75145..76362
                     /locus_tag="VC394_000238"
     CDS             75145..76362
                     /locus_tag="VC394_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP5993738.1"
                     /translation="MEDIIAALSSILSHKMRSILTMLGIIIGIGAIIAIFSIIEGNTE
                     NTKRQLIGGSNNTINIVFNKKSSIDPKFPDKSNAKKPDYLPFMAEEELSKIQQVKGVK
                     NALISYGIDDKVYHLGQKSSAKISAITKNVAEVRRMTFIKGSDFSDKDFIDQKQVIYL
                     EKSLYESLFPKDDGLGKFVEVMGNPFRVIGVFESKEQSGLTSGTEKIAYIPLHQWYNI
                     NGVVDATPEITIQTYRADDLKPVAKRVSDMLNQTIPKSDYMFGVMNLKEFERQLDNLN
                     KSNFVLLAGIASISLIVGGIGVMNIMLVSVTERTREIGIKKALGARRKLILKQFLIEA
                     VILTLLGGVIGVISGMVSGLIITRSLEYPYILSLFSVVLSLAFCCIIGIVFGLLPAIK
                     ASKLDPIEALRFE"
     gene            76456..77109
                     /locus_tag="VC394_000239"
     CDS             76456..77109
                     /locus_tag="VC394_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP5993739.1"
                     /translation="MFKILVVEDDDTISQVICEFLKANNYDPDCVFDGQAALDKWQTT
                     SYDLIILDIMLPSLSGLEVLKTIRKTSDVPIIMLTALDDEYTQLVSFNHLISDYVTKP
                     FSPLILIKRIENVLRVSTPDEKRQIGDLLVDETEHSVYWQGTLVKLTKKEYDIIDYLA
                     KRHQKIVTRDQLMDDIWGYSELDTRVLDNHIKNLRKKMTGIPLKTITGMGYLLGERE"
     gene            77106..78494
                     /locus_tag="VC394_000240"
     CDS             77106..78494
                     /locus_tag="VC394_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEP5993740.1"
                     /translation="MRLIKKTFLVINGLIIVVVTSILLVLYFAMPIYYTKVKDKEVKR
                     EFDQTSKQIKGKTVTEIRDILTKKINKDNIWYSLVDSDNQLLYPSLQLLDGVSESKDS
                     QNVNIVTTFDNSYSNVKVMSQKVTLRDGKKMTLLGQSSLQPVTDASKVLLDLYPSLLM
                     FSVTVGSIVAYLYSRTSSRRILSMSQTAKKMVNLEPNLTCTIHGKDEIAMLASDINRL
                     YASLSTSIKSLQKEYEKASDSEREKSEFLRMTSHELKTPITSVIGMIDGMLYNVGDFA
                     DRDKYLRKCRDVLEGQAQLVQSILSLSKIETLASQNQELFSLKSSLEEEMEVFLVLSE
                     LKHLKVTINLEEQFVKANKVYLLKAIKNIIDNAFHYTKSGGQVMIQLKDNQLVIKNEA
                     ETLLTQQQMKQLFQPFYRPDYSRNRKDGGTGLGLFITHQILDQHHLAYRFVVLDQRWM
                     VFTIDFPSHHDD"
     gene            78596..80221
                     /locus_tag="VC394_000241"
     CDS             78596..80221
                     /locus_tag="VC394_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEP5993741.1"
                     /translation="MKKRKLLAVTLLSTILLNSAVPLVVADTSLRNNTSSTDQPTTAD
                     TDTDDESETAKKDKKSKETASQHDTQKDHKSSHTHLTPPSNDTKQTDQASSEATDKPN
                     KDKNDTKQPDSSDQSTPSPKDQSSQKESQNKDGRPTPSPDQQKDQTPDKTPEKSADKT
                     PEKGPEKATDKTPEPNRDAPKPIQPPLAAAPVFAPWRESDKDLSKLKPSSRSSAAYVR
                     HWTGDSAYTHNLLSRRYGITAEQLDGFLNSLGIHYDKERLNGKRLLEWEKLTGLDVRA
                     IVAIAMAESSLGTQGVAKEKGANMFGYGAFDFNPNNAKKYSDEVAIRHMVEDTIIANK
                     NQTFERQDLKAKKWSLGQLDTLIDGGVYFTDTSGSGQRRADIMIKLDQWIDDHGSTPE
                     IPEHLKITSGTQFSEVPVGYKRSQPQNVLTYKSETYSFGQCTWYAYNRVKELGYQVDR
                     YMGNGGDWQRKPGFVTTHKPKVGYVVSFAPGQAGADATYGHVAVVEQIKEDGSILISE
                     SNVMGLGTISYRTFTAEQARLLTYVVGDKLPRP"
     gene            80299..80550
                     /locus_tag="VC394_000242"
     CDS             80299..80550
                     /locus_tag="VC394_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5993742.1"
                     /translation="MIKKVTTPSQKIKKRVRNGYLLKLGTACLLLSILSYGIGLLGQP
                     SMENTFMGIASVAMLGSVCFFIIFALNRIFDALEDNLRD"
     gene            81226..>81889
                     /locus_tag="VC394_000243"
     CDS             81226..>81889
                     /locus_tag="VC394_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEP5993743.1"
                     /translation="MHVSKLFTSQQWRELKLISYLTENSNAIGVKDKELSKALNISML
                     TLQSCLTNMQFMKEVGGITYKDGYINIWYHQCCGLQEVYQKALRESPSLKLLELLFFR
                     DFSSLEELAEELFVSLSTLKRLIKKTNTYLSHTFAISIVTSPVQVSGDERQIRLFYLK
                     YFSEAYKISEWPFGDILNLKNCERLLSLLIKEVDVKVHFTLFQHLKILSGVNLIRYYK
                     GYS"
BASE COUNT        25552 a        15877 c        17356 g        23104 t
ORIGIN      
        1 aaaagtttta ttgtttatca gaaaaagtaa tctaatatca atatattgtg ttcgagcaaa
       61 aaacttgaca caatatattg atttttttag ttgtcgtgat ataatagttc cgtaaggaga
      121 tttgatatgg taagtttaga agaagacaag gtgactgttc aacctgatat taaagtgatt
      181 aaacgagatg gtcgccttgt taattttgat agtacaaaaa tctatagtgc tttattaaaa
      241 gcaagcatga aagtaactcg gatgtcgcca cttgttgagg ctaaattaga ggctatttct
      301 gatcgcatta tagcagaaat tattgagcgt tttccaacta atatcaaaat ttatgaaatc
      361 caaaatattg tagagcataa gcttcttgca gctaatgaat atgctattgc aaaagaatac
      421 attaattatc gtactcagcg tgactttgca cgttcacaag caacagatat caatttttct
      481 attgataaat taattaataa agatcaaaca gttgttaatg aaaatgctaa caaagatagc
      541 gatgttttta atactcaacg agatttaact gctggaatcg tagggaaatc gattggttta
      601 aaaatgttac cttcgcatgt tgctaatgct catcaaaaag gagatatcca ttaccatgat
      661 ttggattaca gtccttatac accgatgacg aactgctgtt taattgactt taagggcatg
      721 ttagccaatg gctttaaaat tggtaatgct gaagtggaaa gtcccaagtc tattcaaact
      781 gcaacagctc agatctcaca gattattgcg aatgtagcat caagtcagta cggcggatgc
      841 acagctgatc gcattgacga gtttttagcc ccatatgcgg agcttaactt taaaaaacat
      901 atggctgatg ctaagaaatg gatcgttgag actaagagag aaagctatgc ttttgaaaag
      961 actcaaaaag atatttatga tgcgatgcag tctttggagt atgaaattaa tacgctcttt
     1021 acgtctaatg gtcaaacacc atttacttct ttaggatttg gtttggggac gtcttggttt
     1081 gaacgtgaga ttcaaaaagc tattttgacc attcggatta atggtcttgg tagtgaacat
     1141 cgcacggcta ttttccctaa attaattttc acggttaaac gtggcttgaa tttagaacca
     1201 gattcaccaa actatgatat taagactttg gctttagaat gtgcgactaa gcggatgtac
     1261 ccggatatgt tatcttatga taaaattatt gatttgacag gatctttcaa atctccaatg
     1321 ggatgccgct ctttccttca aggctggaaa gatgaaaatg ggcaagatgt gacctcaggc
     1381 cgtatgaatc ttggggttgt caccctcaat ttacctcgca ttgccatgga atcaaatggc
     1441 gatatggata agttttggga gctgtttaat gagaggatgc taattagtaa ggatgcttta
     1501 atttatcgtg tcgaacgtgt cacagaagca aaaccagcaa atgcccctat tctttatcaa
     1561 tatggtgctt ttggaaagcg tttggagaag acagggaatg taaatgatct ctttaagaat
     1621 cgtcgtgcaa cagtctctct tggctatatt ggtctttatg aagtggcgtc tgttttttat
     1681 ggtggtcaat gggaaggtaa tccagatgct aaagctttta ccttgtcaat tatcaaggca
     1741 atgaaacagg cctgtgagga ttggtcagat gaatatggtt atcatttctc tgtttattcg
     1801 actccatcag aaagtttgac agatcgcttt tgtcgtttag atacggaaaa atttggcatt
     1861 gtgacagata ttacggataa agaatactat acaaattctt ttcactatga tgtgcgtaag
     1921 agtccgacac cttttgaaaa attagatttt gaaaaagatt atccagaagc aggtgcttca
     1981 ggtggtttta tccactactg tgagtatcct gttttgcaac aaaatccaaa ggccttggaa
     2041 gcggtttggg actatgctta tgatcgtgtg gggtatttgg gaaccaatac gcctattgat
     2101 aaatgctata attgccaatt tgaaggcgat tttaccccaa cagaacgtgg ttttacttgc
     2161 ccaaactgtg gcaataatga ccctaaaaca gttgatgtgg tcaaacgtac atgtggttac
     2221 ttggggaatc ctcaggcccg cccaatggtt aacggtcgcc ataaggaaat ctctgcgcgt
     2281 gtaaaacata tgaatggttc tactataaaa tacccaggcc tgtaaactca agctggggga
     2341 gagccctttt gatggaaata tgatatgctt aaaaggttgc ttcatgcaag caatctttta
     2401 ttgtttaatg gtttcttagc aattgtgaga taagaataac taatccaaaa cgactaatta
     2461 gctgttagca atcggactat aagaagaaag agataaggaa gaaataatgg gaaaatatca
     2521 attagactat aaaggaatgc agcaggtaga acgctttcat gagaagcatt ctaaaaaaaa
     2581 gacggataaa aaatcccgcg ttcaagaact taaggctcgg tttttagaga agtcaaaaaa
     2641 acaggataac taaagtatcc aataaagaaa gaaggttgag tatgcttaat attgggattg
     2701 tgggactagg tgccatttca caaaaagcct atttaccata tatgagacag ctgagtgaca
     2761 ttacttggca tctatcaacg cggaatgcag cagtacgtca gcaggtcggt cagttatttg
     2821 gtcacgctat tctttacagt gatgtcaagg agttgtcaaa ggcaagttta gatggcgtct
     2881 ttattcatgc ggccacatcg gcccacgcag agttggctag tttattttta aatcagggga
     2941 tcccagtctt tatggataaa cctatagctg acaattacct gatgacaaag aacctctatg
     3001 acttagccaa agaaaatcag acttttctga tggcaggatt taacagacgt tttacacctc
     3061 gtgtcaagaa gttgtcaagt ttgtcaacta agcgtaaagt ggctgttgaa aaaaatgact
     3121 tgaaccggcc aggagatatg acttttaagc tctttgattt ctttattcat cctttagata
     3181 cagccttatt cttgtcagaa gggaccttac taaaagggca ttttcaatat catctggaag
     3241 caggcttact gagtcaagtt atggtaacct tgatgaccga aagcatgacg actacagctt
     3301 ctatgaattt acaatcaggg agtcgccgtg aggtaatgga agttcaacgg gctgaagaaa
     3361 cttatcattt agagaactta gacgagttat ctatttataa aggtactgaa aaaagggtgc
     3421 ttggctttgc ttcttgggac actaccttgc acaaaagagg ttttgaaaca atgatcgatg
     3481 cctttttaga agctattagc acaggcgtca atcctgtcag tcctgagtct agtcttttaa
     3541 gccactggat ttgtcaacag attgctgact ctcagctttc ttatggagag ttaacggtag
     3601 agttgcctaa ggattaggaa ttgatatgga aataagacga ccgactttaa aagataaaga
     3661 cgctgtattg tcaatgatta acgaattttt agagcagaaa agtgctacag atgggttatg
     3721 gcattttaac gtcaatgact ttaactatga aacatggttg gaagattcct tgcgacagga
     3781 aatgggtttg tccagtcaag gagtgcctgc tattcaatac gtcgcatttg atgagagaag
     3841 tcaggctatc gggttcttaa atcttcgttt acggttaaat gagagattac ttgaaaaagg
     3901 tgggcatatt ggttactctg tccgtcccag ccagcgtggg aaaggttatg ccaaggagat
     3961 gcttaaacaa gctgtcagct atgcgatttc caagaatatc acgacaattt tggtaacttg
     4021 tgatgagact aatgttgcaa gccgagcagt tattgtggct aacggtggtg tcttggaaga
     4081 tagtcgaggt ggaacggaac gttattggat tgaagaacaa agaggcgata agtgatggca
     4141 gaaaagtgtt ggaataatcc taagccaaag gaatggcagg cagaggagtt aagtcaaggc
     4201 cggattattg actataaggc ttttaacttt gttgatggtg aaggagttcg taattccctt
     4261 tatgtgtctg gttgtttatt ccattgcaag gggtgttata atgctgcgac ttggtctttt
     4321 aaggcgggaa tgccttatac gcaagaactt gaagagcaga taatgacgga tttggcacag
     4381 ccttatgttc agggactgac acttttaggt ggagaaccct ttttgaatac gggtatctta
     4441 attcctctga ttaagcgtat tcggcgtgaa ttgccagaaa aagatatttg gtcttggacg
     4501 ggttatactt gggaagaaat gatgcttgaa acaccagaca aactggaaat gctgtcctta
     4561 attgatattc tggtagatgg tcgttttgat atcacaaaaa agaacctcat gttgcaattt
     4621 agaggctctt ctaaccagcg aattatcgat gttcaaaagt ctttggctgc taaagaagtg
     4681 attatctggg ataagttaaa cgatggggac caaacctttg aacaaattag tcgggaggat
     4741 ttgctttaag aagctgactt atccacatga tttttttgaa aaggtcatgt gaaaacaggt
     4801 cctttaaggg tgaattgggg agaaagtaat tgttatttcc tggttttccc cacattatcc
     4861 tcatagttat ccacaggttg tgtataagtt gtagaattgt gaataaagac taaaaaagag
     4921 gcctaggcct cttttttgat atagacaaat tcatgatctg ttgataggtc tggatggtag
     4981 taaaaaccag caaagacaag gcttttaagg ctgtcaacag tttggcaatt attttccata
     5041 caggcagtga gaaatgcacc tcgtgccttt tttgaaatgg tggaatgggt tttgaattgg
     5101 ccctcttttt ctgccataaa cttgggactt atccacaatt gtttacagtc tttggaaaag
     5161 acatcgtcaa attcactgga taaaagggaa atcacttggg gatgctcttt ggcaaactga
     5221 ttataacagg gccgccagta agacttgagg ctttgtcctt caatttttat tctggtgtga
     5281 aaatcatggc ggtgctctgc gataggatga ttagcgggaa tgataccgta aaaggatgag
     5341 gtgatgtaaa cttgctgggt gaggtaagcc tgttcttggg tagtgagctt atcgcgcttg
     5401 atatggcggt acataaggcc attgaacagt tggtaggcag ggtaagccaa gttttgttga
     5461 gaggccatgt cttgccagcg ttgctgttct tttttagctg cctcttcttt gatacgatag
     5521 gatttggcca agtcctccgt ggtcatagct gccatgattt ttaaaatggc ttggctgtct
     5581 tgtggaagga ggtgtgggtg agattcctta gggatcatca tttcttttgc ggttggaatt
     5641 aaaaaggtta gcatagtgtt attgtagtaa agttacttgc tttctgtcaa gaaaactagt
     5701 cttgtccaag gatgacgttc acacagatac cttcctcgtc ttctgtcgtg aaagaagaag
     5761 aatcttcttg taaaatagct aggccgtgta atcttgcttt tttcagagta gctgaaaaaa
     5821 gcaggggcgt ctcaatgtga atggtcaaga aggctaagcc tggggcacct tcttggtgtt
     5881 ttttgaggta aggggctgac cagtgattga atgcaaggtg atgatggtag ttccctgaag
     5941 caatccagct agcacttgga atggtcattt tatcgcctaa atcaaaaacc ttttgataga
     6001 gaagtgaaga agccagagca tttttgacac tcagatggac atggccaatg cgtgtgtctt
     6061 gagctagtaa aaagcgcttg gggatatctg tcaactgctc caatatgctt ttggtatccg
     6121 tgggctcagt cacaccaata atttgaccat tgtcgcggat atcccaatgc tcaacagctt
     6181 tgtcatgata aatttcaatc ccattgccct caggaccgct gaggtagata gcttcgctat
     6241 agccgtgatc agctgatcct tctaaagaga tacctctggt taagaaatga ttgagaacaa
     6301 gtcccaagct atgacgatca ggaactaaaa aggatgtgtg ataaagacca taagccttat
     6361 ttgctggtaa aggtgtttgt cgtaactcta agataacggt tttaccgtct gtagtcaatt
     6421 gacgagatgt cgtgtcttgt gagagaacct gtaaaccgat aatacttgtg taaaaggttg
     6481 tcatctttgc tagatcggta acatttagag aaaccgttcc taaagagatg gttgagttgt
     6541 aagggtatgt catcctatta cctccaaaaa ctaattaaat ctatcataat caatcagttt
     6601 caaaaagaaa agtaggcaca aataagtggt ataggcacta aaaagtaact catttgtcag
     6661 ccctatcttg tttctgttct ctctaatagt ctataatggg ttataagtta caaaaagata
     6721 ctaagaggag ctgctgccta tgctgaaaaa aagaccggct tgtcccgtag aaacaacctt
     6781 atcggtaatc gggaataaat agaaattatt aatattaagg gacttgctaa agggaccttg
     6841 cgttttgggc agttgaagtc ctcaataggt ttggtcagtc aaaaggtact tactgcacaa
     6901 ttaagggcta tggaagcaga cggattggtt catcgggaag tgtatgcaga agtgccacct
     6961 cgggtggagt attccttaac agaaactggg ttgagcttag ctcctgttat tgaagcgatg
     7021 tcggattggg gccaaactta ccaagaaaaa catgccgatt tatttgaaga aggctagagg
     7081 aaagagttgt ccgagcatac tgagacttag agaagctgat agtttttaaa aaatgatatt
     7141 atcaaattct ttacagtttg ataaaattac tttttttcta caaaaagact tgcgctcatc
     7201 tcacgaatat gataatataa tatcaagtat gtttgagaaa acagaagctc aggtcaagta
     7261 agtgcacact tacaataaca cagttgataa tggggaaact cagagtcaag aaaaagaaaa
     7321 gcaagccttg cattttgcaa ggcttttttc tgtctttatt ttggtctgcg ccgagcaaaa
     7381 tctaaaaatt taaatttttc cagtttatgg cgactctcgg tgaattggaa ttggttatta
     7441 ttggaaaggt agactttgga tttgacggaa acaacatgtt ggtctgatcc aaggtcaagt
     7501 aaaattttat cacgatcggt tatttggtca atggtaacct ctttgagggc aaagtcaatg
     7561 gcgagatgga gttccttttc caaatattga taaatagaat gctctgcgat ttcccttgtc
     7621 attattggca ccaaagtttt actaaggtaa tcaatgtcta agacggaagc cacaccctca
     7681 acaacgcgtt ggcgtgtaac acgccagacc aagctgtttt tgctaaaacc ggtcaatttt
     7741 gaaagtgtct catccacaat cagtttatca atggcaatga cgttggtctt agaatctaga
     7801 tttgagtaag agacgagttc ctgataacta gttagttctg agatagggaa catgatctga
     7861 tgatgtttaa tcacttgggt gccacgtcct tgttttttga ggataagacc tgcctttgtg
     7921 agaagtgaga gagccttgcg gacggtatct cggctagctt ggtattgttg actcaattct
     7981 atttcagtag gtaaaaaatc cccttctttg taaaagtctt tgtttatttt tgtttcgagg
     8041 tctttataaa tacgttcata ctttgtcata gggtaaatta cctttccatt tgtctatttt
     8101 tttacagaca acacctttta ttttaccaaa ataatggctt ttttggcaca aacaacttgt
     8161 agacaaattt gcaatcgatt acaataaagg tgtcagttag attctatcta agaatttgaa
     8221 tatttcattt tgacgttgct tgttaaaagc aactagaaca aaggactagg ggatgtcaca
     8281 tgggaaaatt tgaacaggat gctaagagtc ttctaactgc tattggcggt aaagaaaaca
     8341 tcaaggctgt cacacactgt gcaacgcgta tgcgttttgt tttgaatgat aataataagg
     8401 caaatgtcaa agagattgaa aaaatctctg tagttaaagg gacatttacc aatgctgggc
     8461 agtttcaggt aatcattggt aatgatgttc cagtttttta taatgacttt acagctgttt
     8521 ctggtattga aggggtgtct aaagaagctg ccaaatcagc agctaaaagt aatcaaaatg
     8581 ccttacaaca ggtgatgacc atgttggctg agattttcac acctattatt ccggcgatta
     8641 ttgttggggg gcttatttta ggtttccgta atattttgga gagtgtgcct tttgaatttc
     8701 ttgggcagca ggtcgaaaaa gggaaattag tttttgatgc agctggggat cctgtttgga
     8761 atacgattgt gagggtatct cctttctggt caggggttaa ccatttcttg tggttaccag
     8821 gggaagctat tttccacttc ttaccagttg ggattacttg gtctgtgacg cgtaagatgg
     8881 gaaccactca aattttaggg actgtccttg gtatctgttt ggtgtcacca caattattga
     8941 atgcttatgc ggtagcagga acgcctgctg ctgagattgc caaaaactgg gtttgggatt
     9001 ttggtttctt taccattaat cgtattgggt atcaggcaca ggttattcca gcccttttag
     9061 ctggtctgtc ccttgcttat cttgaaattt tctggcgtaa acggattcca gaagtggtct
     9121 caatgatttt tgtgccattc ctttctttga ttccagcttt gattttagcg catacggtat
     9181 tggggccaat cggttggact attggtaaag ggatttcctt tgttgtgtta gctggtttga
     9241 ctggtcctgt taaatggcta ttcggtgcta tctttggtgc cttgtatgct ccgctagtta
     9301 ttactggttt acatcacatg acaaatgcca ttgataccca attaattgct gatactgcaa
     9361 ctcgtacaac tggtttgtgg ccaatgattg ctctttcaaa tatcgctcaa gggtcagccg
     9421 tttttgctta ttatttaatg aatcgtcatg aagaacgtga ggctgaaata tcgcttcctg
     9481 cagcaatttc tgcttacctt ggggtaactg agcctgcctt atttggggtt aatgttaaat
     9541 acgtttatcc ctttgtagcc ggaatgatcg gatcaggtat tgcgggtctc ttatcaacaa
     9601 cctttaatgt tcaggcaaat tctattggtg ttggtggctt accaggtttc atggctatca
     9661 atgtgaagta catgattcca ttcttcatct gtatggtagt agccattgtg gtgccgatgt
     9721 ttttagcctt ctttttccgt aaatcacata tcatgactaa gacagaagat gaagctaaac
     9781 tacctgagac acccgtttcg gatgctcctg tagcaactgc tccacataag actatgcaag
     9841 gaacagttat cactttaaca agccctttaa cgggtgaagt taaagcgttg tctgaagctg
     9901 ttgatcctgt ctttgcacag ggagttatgg gccaaggtgc tcttcttcaa ccgacagaag
     9961 gggtgttagt agcgccttgt gatgctgaag tatcggtctt gttcccaact aaacacgcta
    10021 tttgtttggt gacgactgaa ggtttggaat tattgatgca tattggcatg gatacggtta
    10081 acttagatgg tcaaggattt gaagctttgg tgaagcaagg tgatcaggtt aaggctggac
    10141 aaacattgat tcaatttgat atagcagcta tttctgaagc tggatacgcc actgaaacgc
    10201 ctcttgtggt gactaatcaa gatgctttta cggtaactgt tgaaggtagt ttaccgcgtc
    10261 agattaaggc taatgataag ttagcagtag cgatgaaaaa gtaggtttac tagggagaaa
    10321 acgttcagga actgagatca gttcctgaaa ctattttagg gaggaaaact catgacaatt
    10381 gataaaaaga aagtcgtcta tcaaatttac ccaaaatcct ataaggacac gactggaaat
    10441 ggtgtgggag acttgcgagg gatcattgat aaattgcctt acttacaaga actaggaata
    10501 gatatgattt ggttgaaccc tttctaccct agtccacaac gagacaatgg ctatgatgtt
    10561 tcagattata cggcggtcaa ccctgatttt gggacaatgg ctgattttga agatttggta
    10621 aaagctgcta aggagcatca gattgagtta atgttggata tggttttgaa tcactgttcc
    10681 acagaccacg agtggttcca aaaagcttta gcaggagacc cttattatca ggatttcttt
    10741 atcttgagag atcagccgac tgattgggtt tccaaatttg gtgggaatgc ttgggcgcct
    10801 tttggagata caggcaaata ctacttacac ttgtttgatg tgacacaggc tgacttgaat
    10861 tggcggaacc cacatgttcg tgaggaattg gctaaagtgg ttaatttttg gcgagataaa
    10921 ggagtgaagg gattccggtt tgatgtgatt aatctgattg ggaaagatga agagctggtg
    10981 gattgtccgg tcaatgatgg taagccagct tatacggatc gtcctattac tcacacttat
    11041 cttcatgatc tcaatcaagc cagttttggt caggatgatt cgtttatgac agtaggggaa
    11101 atgtctgcca cgactattga taactgtctt ttatacacgg ctcccgaacg tgaggagcta
    11161 tccatggctt ttaatttcca ccatctaaaa gttgattatg agaacggtca gaaatggact
    11221 attatggctt ttgattttgc agcgctgcga gacttattcc atgcttgggg tgaaggcatg
    11281 agtcagggca atgggtggaa tgccttgttc tacaataacc atgatcaacc acgtgccctg
    11341 aatcgttttg ttgatgtaac acatttccga aacgaaggtg cgacgatgtt agcggcttcc
    11401 atccatttgt cacgaggaac gccttacatt tatatgggtg aggagattgg catgcttgat
    11461 ccagattttg atagtatgga tgattatgtg gatgtggaaa gtctcaatgc ttactcaagc
    11521 ttattagtct caggtaaaag tgcagaagaa gcctttgcca ttatcaaggc taagtcaagg
    11581 gacaatgcca gaacaccaat gcaatgggat gctagtgaac atgctggctt tacgactggt
    11641 aagccttggt tagaggttgg caaatcttat cgagacatca atgtcgaaac agaaaaagag
    11701 ggacgtattt tccctttcta ccaacgcttg attgctttgc ggaaggaact ccctattatt
    11761 gctgaagggg actatcgggc tgcttttaaa gatagtcagg ctgtctacgc ctttgaacgc
    11821 catttaggtg accagtgttt gcttgttctc aatcatttct atgctggtga ggtcgaactg
    11881 gaattacctc cacgttatca acatggacag gccttaatca gcaactatga gaaagtctct
    11941 atttgtgaaa aagtgatgct gaaaccttat cagacacttg ctattttagc tgataactaa
    12001 ctaaaagcct gaggttaggc ctcaggcttt tggtgattgt cacgaaaaaa gcagttttct
    12061 catatattta tttttaaact cttatttttt ctaaataaaa acaattgaga taagttattt
    12121 tagctaaatt ttggtacaat ggaaaagatt ataagtagag tttaagataa agattctaca
    12181 aatttaaaat gatataagga gatacgaatg acaacttatc aagatgattt ttaccaggct
    12241 gtgaatggaa agtgggctga aacagcggtt attccagacg acaagcctcg aacaggtgga
    12301 ttttcagact tagcagatga aattgaggca ttaatgctag acactacaga tgcttggtta
    12361 gcaggggatc atatccctga tgatgctatt ttaaaaaatt ttgtgaaatt ccatcgtttg
    12421 gtggctgact acgctaaacg tgatgaagtt ggagtaagtc caattctccc tttaattgaa
    12481 gaatatcagt ccttaaaatc tttttcagaa tttgttgcta atatagcgaa atatgaattg
    12541 gcaggtttac caaatgaatt tccctttagt gtggcaccag attttatgaa tgctcagctg
    12601 aatgtgcttt gggcagaagc accaagcatt cttttgccag atacaacata ctacgaagaa
    12661 ggcaacgaaa aagctgagga attgcgtggt atttggcgtc agtcgcaaga aaagctttta
    12721 cctcaatttg gtttttcaac agaagaaatc aaggatcttt tggataaggt aattgagcta
    12781 gataagcaat tggccaagta tgtcttgtct cgtgaggaag gctcagaata tgccaaatta
    12841 taccatcctt atgtgtgggc tgattttaaa aaactagctc cagagcttcc tttggatagc
    12901 atttttgaaa agattttagg ccaagtacct gacaaggtta ttgttcctga ggaacgtttt
    12961 tggacagaat ttgcagccac ttattactct gaagccaatt gggatttact caaggctaat
    13021 ttgattgtgg acgcggctaa tgcttacaat gcttacttga cggatgatat tcgtgtggaa
    13081 tcaggcgctt attcgcgcgc tctttcagga acacctcaag ctatggacaa acaaaaagca
    13141 gccttttatt tggcgcaagg accgtttagc caagcgcttg gcttgtggta tgctggtcaa
    13201 aaattctccc cagaagctaa ggctgatgtg gaaagtaagg tagcacgcat gattgaggtt
    13261 tataagtctc gtctagaaac ggctgattgg ttagcaccag ccacacgtga aaaagccatt
    13321 actaaattaa atgttatcac gccacatatt ggttacccag aaaaattacc agaaacctat
    13381 gctaaaaaag tgattgatga gagcttgtca ttggttgaaa atgcacagaa cttggctaaa
    13441 atcacgattg ctcacacttg gagtaaatgg aacaagccag ttgaccgtag tgagtggcat
    13501 atgccagccc acttagtcaa tgcttattat gatcctcagc aaaatcagat tgtctttcct
    13561 gcggcaatct tacaagagcc attttattct ttagatcaga gttcttcagc caattatgga
    13621 ggaattggtg cagtcattgc gcatgagatt tcacatgcct ttgacaccaa tggtgcttcc
    13681 tttgatgaac atggtagcct aaatgattgg tggacacaag aagactatgc tgcctttaaa
    13741 gagcgtacag ataagattgt ggcgcagttt gacggcttag agtcacatgg tgctaaggtc
    13801 aatggtaaat tgacagtttc agaaaacgtc gctgatctag gtggggtagc ttgtgcttta
    13861 gaagcggcac aatcagaaga agacttctca gcccgtgact tctttattaa ctttgcgacg
    13921 atttggcgta tgaaggcccg tgaggaatac atgcaaatgc tagctagcat tgatgttcat
    13981 gccccgggtg aactgcgaac aaatgtcacc ttaaccaact ttgatgcttt ccatgagacc
    14041 tttgatatta aagaaggcga tgctatgtgg agagctccga aagaccgtgt gattatttgg
    14101 taattaggta gttgataaac cttcaaatgc ctgtgattat aaaaatgact gattctgtaa
    14161 aacacaggat cagttttttg cttccttatt ggtgtccgtt ttgacacaac aaaaagacaa
    14221 ccctcaggaa agaaggttgc cttttttaga cttatcagaa ataagtgatt agttgttaag
    14281 ggcagcagcc atagtagctg caacttctga ttcaaagtcg ttagcttttt tctcgatacc
    14341 ttcaccaact tcaaaacgtg caaaagcaat cgctttagcg tttactgagt caaggtaagc
    14401 ttcaacagtt ttgctgtcgt ccatgatgta aacttgtgca agaagagtgt aagcttggtc
    14461 aactttagtg ttgtcaagca tgaagcggtc cattttacct gggatgattt tgtcccagat
    14521 tttttctggt ttgccttcag cagcaagttc agctttgatg tctgcttcag cagcagtaat
    14581 cacatcgtct gataattgag cttttgaacc gtatttcaag aatggaagag ctggtttgtc
    14641 aaccattgca cgagattcgt tgtcaagttc gatagcgtgg ttcaattgag caagttcatc
    14701 tttgatgaat tgtgcatcaa gctcagtgta tgaaagaaca gttggtttca ttgcagcgat
    14761 atgcattgat acttgtttag caagagcatc gtcgccgcct tcgacaactg agataacccc
    14821 gatacggcca ccattatgtt ggtaagcacc gaagtgttgc tcatctgttt tttcaatcaa
    14881 agcaaaacga cggaatgaga ttttttctcc gatggtagca gttgcattaa cgtaagcttc
    14941 agcaagagtt tcacctgaag gcataacaag tgcaagtgct tcatcgttgt tagctggttt
    15001 gccttcagcg ataactttag cagttgcgtt taccaattca acgaattgag catttttagc
    15061 aacaaagtct gtttcagcgt taacttcaac aacagcagca acgttaccat gaacgtaaac
    15121 gcctgttaaa ccttcagcgg caacacggtc agctttttta gctgctttag ccattccttt
    15181 ttcacgaaga agttcaacgg ctttgtccat gtcaccatct gtttcaacaa gtgctttttt
    15241 agcgtccata acgccagcac cagatttttc acgtaattct tttacaagct tagctgtaat
    15301 ttctgccatg tgtagtatcc tccaaatgat atttgttttg gtttcaaaaa acgagacaga
    15361 gcggtttgct ttctgccccg tttggttttc gattgttaaa gacaaacaaa gtttgccata
    15421 agagttagga actctgtgct aattaagcgt tgtcgccttc gacaacttca acgatttctt
    15481 cgattgaatc tgcttgaatg tcagcttcaa aagcaacgtc tgcatcttca ccttgacggc
    15541 cttcgataat agcgtcagct aatttagcgg tgattaattt aacggcgcgg atagcgtcat
    15601 cgttagctgg aatgatgata tcgatatcat ctggatcagc gtttgtatca accatcgcta
    15661 cgactgggat accaagtttt ttagcttctt taacagcaat ttgttcttta tgtgggtcaa
    15721 caacatacat cacgtctggg atacgaggca tatcttcgat accgcccaag aatttttcaa
    15781 gacgagcgcg ttgtttgtta agaagtgcaa cttctttctt aggaagaacg tcaaaggttc
    15841 cttcttcttc catacgtttg atttctttca aacgagcgat acgtttttgg atagttcccc
    15901 agttggtaag cgttccaccc aaccaacggt ggttgatgaa gtattggcct gcacgcgttg
    15961 cttcgtcagc aactgcttca gcagcttgtt tcttagtacc aacgaacaag atgacagcat
    16021 cgtttgctgc agcatcacga acaaattcgt aagcttggtc agctaatttt acagtttgtt
    16081 gaagatcgat aacgtggata ccgttacgtt ctgtgaagat gtatttagcc atcttagggt
    16141 tccagcgacg agtttggtga ccaaagtgaa caccagcctc aagaagttgt ttcattgaaa
    16201 ttactgccat gagtagtttc tcctttataa aatgtttttt cctcttccag acttcgactt
    16261 gcaggctaac ctagcggcaa caagcccaca attggtctag aatgagtatt tgccgcctac
    16321 acgaccctac tatattatca aaaaaagagg ttaaaagcaa ggagaaatgc tatggattcc
    16381 cttatttttg agatgactag cttctttgtt tattggtaag tgttaagaca agcttttgct
    16441 cattttgggt gacacttgcc aaatcgtttt atttaagata tagtgaactt gactaatttt
    16501 tggaggcgtt ttcatgacga agggtattcg atttcaattg cttggcagtc cccacatttt
    16561 tcttgatgac aaagaacaat tttttgcctt tgccaaagca aatgctctcc tttattactt
    16621 agtggttaat ggatctgtta gccgcgaggt ggcggcgagt ctactttggg aaaataagaa
    16681 cacgcaaaca gcgaaaaaga atttacgaaa cgctatctac caggtcaata aagtcttgca
    16741 agctgatgtg attatttgtc ccaatcgcaa cctgctggtt ttgaaaaaaa cacttgatat
    16801 caaaacagat attaaccttt ttctggctga tccgctagct cacttagaac tctaccaagg
    16861 ggagtttttg cagggctttt accttaaaag tggtgaggaa tttgatcttt gggtttcaaa
    16921 aatgcgtatg cagtatgaac aggtatacct aaaagcgtgt taccaaaaaa ttgaagaaaa
    16981 gctctcttta gatgccattg aagatgtcga ggaacattta aagcagctta ttgaacgtga
    17041 tgaatttgaa gagaaaaatt atcagctctt gatgaggctt tatcagcagg ggaattgtcc
    17101 tggtaaggtc atcgaaactt attatcagtt ggctaatgtg ttggataagg agctagggat
    17161 tcagccaagt ttgcagagcc aacagattta tcaggaagtg gtcgctaaag atcgtaatga
    17221 acgcaaaatc aagcattttt tgcgcaacag taatcatttt ttggggcgga ttgatgagat
    17281 taagcagctg gaaaactatt ttgccaactg tttggcctgc caagaggtag gagctctgct
    17341 cttaattggt gatacaggga ttgggaaacg aaccctagcc agacaggtct tagccaatca
    17401 aacccaaacg tttcaaattg tcacagctaa atgttttcgt gaagaggcta tggattcctt
    17461 attgccttgg cgtaatatct tagatggcct gggagatttg gtgattcaaa accgcttatt
    17521 gaccaccaaa gcttggaagg ctgctcttaa acgctgtttt cctgtggcaa ccatttttca
    17581 agaagataac aaccaaccct ttatcaagga ccacaccagc ttgttggttt cctttattgt
    17641 tgatatttta caacatttgg cagaaatcaa ggcgctggta atccttattg aggattgtca
    17701 ctggatggat gaggatagtt tgaccttgtt gcaacgggtc atgaatcaat tggtccacta
    17761 tccgattgct tttgtcttga ccaagcatct aggaacgact cctgagctcg gtctttgttt
    17821 gaatgctttg atgagtcagg ggcgtttaga aagcatttgt ttggagcctt ttaatcggca
    17881 agaaagtttg gcatatatta acagtcaact gggtagtcag ccagtgacgg aagaagaaat
    17941 ggagcacctc tatcaagcca gccaaggcaa ccctttcttt ttgtcagaat acactcagac
    18001 cctactgcgt cacgaaaaat ttgtgcctct aacgcctgcc attaaggcta agttgggtct
    18061 taaactagct aacctaagta gtcgtgatga cgcattgtta aactatttgt cttgttgtcg
    18121 gaggcctatc cctctaaata ccctcgctca attgatgtta ctgcctttag aagaagtgat
    18181 cgagatggtg gataatctgg ggcactacta tattttggtg gaagaaagcg taggagagga
    18241 agtgttgatt tcatttcgtc agcgaattat tcaactctat agttatgacc gtttatcttt
    18301 gtcaaaaagg cgtttattgc acggacaaat tgccaaacgg ttagaagatt tactgcctat
    18361 tttgacacca agccctcact tattagatga cattgcttac cattatcagg aatcacggca
    18421 ggtgattaag gctttggaat acaaccttaa ctatctagat gctaccttgc catttcagca
    18481 tgagctgttt cccatttatt caaagagtat tggctccttg gaaaagtcag atcgtgacca
    18541 tcaacgctta atggaagagc agtttgatag gattcgtcaa agcattgcgg atttggaact
    18601 gacctatgat aataaccgtg atttccagca gttgcttatt cgtttttctt accttgaagg
    18661 ccgttatgat attcgaacag ggaggtatca agaagggatt aaaaacattc aaaaggtcat
    18721 cgctttggca accgagctaa aacagccctc cttcttgcta gaaggctatc gccagttaat
    18781 tcattactgt atccaagtgg aaaacaagcc agaaatgcgt tattacacta gtctttcatt
    18841 ggaggcagcg gttgctgcta atcattttga ggccattgcg attagtttgc ggttaaacgg
    18901 tctttatcat ttgattattg gggagttaaa tgaggcagag cgtctgcttc aacagtcgat
    18961 tgacttcttt aaggtgacgc caggcttgca agctaactat gcgattcaga ttgctgctgc
    19021 cttagattat ttgggggaga tcgcccagat tcgctatcag tttgaaaagg ccgttgctta
    19081 ccaaaaacaa gccattgcct tgaccgaaaa caagccagct gagttatcag tgagtatttt
    19141 ttatattgga ttagggattt cctactttta tttagctgat tttgaacaag ctgagcaaat
    19201 cttgagtttg gctaaggaag ctttggtcaa tcatagttat ccttggaaag aaacgcaact
    19261 tcaaatttac ctggccatga ttcaatggaa aaagggcaat tatcagccag ccttgaccct
    19321 acttgattac agagaaacct tgatgtctag gtatcgcaat ccgcgtgata agggcttggt
    19381 tttctatttg atggctgtgg tcaagtacca attgatattt caaggagcta ccctaagtca
    19441 gcaggaaaaa gaaatggcag actacctgtt gtcagaatcc tttgagtatt attatgaaat
    19501 tgctagtact aacctcaacc cttaccgaga ttgtcatttg gtgagtgagt tgaacgattt
    19561 gcgtcaacaa ttatctgcta aaagctaagg aaaaccctct gattcacatc agagggtttt
    19621 agttatgttg tgccataatc tgtacgaggt aaaagataaa ggtggctgct aaattggcgg
    19681 tgtggttatc aatatcgtgt ggtggtgaaa cctcaaccac atcaaagcca actaattttc
    19741 cgctagctgc aatgtgttgc aaaactaaga cggcaagatt tgggtcaaca ccgagggact
    19801 ggatagcact aaccccaggt gcagcaccaa ctgagaaaca atccatgtcg atagtcaggt
    19861 agaccctttc ttgcccttct aagaaacggt caatggctcg gcagaccttt tgatggccca
    19921 tttggtagat gtcttgacct gttaaaaatt gaatgccctt ggattttgcc acaaagtcaa
    19981 ataggaaaag gttgttatta tgttcttgaa tgcctaaaac gaagtattta aagaggcgtt
    20041 tgtcagcaac tgcatcgtca aacatttgtc gaaagcctgt ccctgaatta gggcctgttt
    20101 ggtcgtaagg ccttaaatca aagtgggcgt ccatattgat gacagctagg tcatcacttg
    20161 gggataggct ttggcgtagg cctaagtagt ggccgtaggc ggtttcatgg ccacctccta
    20221 agacgatggg tttgagattg aggtcacaca tccgtttgat ggctttgctt aaactgtttt
    20281 gcagttgttc caaagagcga ttggggccgt caatgttacc cacatcgtaa accataactt
    20341 ggtttcctaa atgccaaggg aatttggcta gctgggttcg tatggcagct ggactttcga
    20401 ccgctccgac acgaccatta ttgatataaa cgcccttatc acttttaaag ccaatcaagg
    20461 caaaatgcgt cccttcaaat ggggttagtg agctgtcgtt aaggtcaagg aaagtcatga
    20521 ccatgcccca tttggcagta tataagtcat cgtcgatccc accatgataa tagctagtgg
    20581 tggatgggta gtaatcttct agcatgcgcg tcatctcctt taggattatg atagcaaaat
    20641 aaaacttaca gtcaacactt tcttggcctt tgtcactggt ttttaggaat agcagagcaa
    20701 taaaatcgtc tgcttttggt cccagttagt tagtctaaag aggccatgac ctctgccaca
    20761 gcccctctag ttaaagaagt accttattca ctaaaattgg atactcaact caactgcctg
    20821 ctcaaccgct tcgaggaagc ttgggtcttc aatgactgct gatgccttgt ttagttcatc
    20881 gtagatttcg atgtgtttat cgtgttcgat aaagttgatt tccttgcgga agagatcgta
    20941 ggcaacctta gtccctttac ctagttcatg attttcaggt ttgagatcaa gagcttggca
    21001 ggctgccatg atttcagtag ccacgatgcg gcgagaattt ttaaggattt caaaggcctt
    21061 gcgagcagcg gtagtgccca tgctgacaaa gtcttcttgg ttttcacaag atgggataga
    21121 atccacgctg gctggatgtg ccaaaacctt gttttcagaa gcaagagagg cacaagcgta
    21181 ctgggtaatc ataaagcctg aattgagacc tgggtatttg acaagaaagg atggcaattt
    21241 gctgagctgg ctattgacca aacgttccac gcggcgttca gaaacatttc caatttcaga
    21301 aatggcgatg cctaagaagt caaatggctg tgccattggt tctccgtgga aattacctcc
    21361 tgaaataacg tggccatctt tacagataat tggattatct gtgacagaat tgatttcaat
    21421 gtcaactttg gatttgacat aggcgatact gtctttgcta gccccatgaa tttgaggcat
    21481 gcagcgcaag gtgtaagggt cttgcacacg agattgagta gctacggttg tgttttggct
    21541 gccttcaatg aggtttcgga tattgcgggc tgttgccaac tggccgcttt gtgggcgaat
    21601 ggtgtgaagg ttttcttcaa atggactagt aatcccgtta tgcacttcaa gggatagggc
    21661 accagctagg tctgacagtt tcaataattg aatggcgtca taggttgcaa gggcaccgat
    21721 tgcagtgaga acagttgttc cgttgataag ggcgagtcct tctttagcgg ctaaagaaat
    21781 cttgtcaata ccagctttat caagagcttc ttgaccagaa agaagctctc ctttgtagta
    21841 agctttccct agaccaagca taggaagcac catgtgagca agcggcgcca aatctcctga
    21901 agcccctaga gacccttttt caggaatgta aggatgaacc cttttgttga gcagttccaa
    21961 cagcttttca atggtggata gacgaatacc agaataccct ttgacgagag agttgatacg
    22021 gatcagcatg atagctctca cggcatcttc tggcaatggg tcaccaaatc cactagcatg
    22081 ggtacgaatc aaattttctt ggagttggac tgtatcttct ggtgagatgc ttacgttaca
    22141 aagggatcca aaaccagtgg tcaccccgta aaccacccgt ttttcactga caatgtcatc
    22201 aacaatttta cgagaggcat tcacagcttc aatagcacta tcatcaatgc gacaggctac
    22261 gccttggcga gcaatcgcaa tcacgtcttc gatagtaagg ctttcgccat ctaaattaat
    22321 cactcttgtc atagtttttc tcctagattt gttccttagg gggcatttgg tccatttggt
    22381 ggttacgtcc gtgcataagg tagtacacac tacttgcaat gatgacacca actgcaccat
    22441 aaacaaagtt cattgggttg tcaccccaaa tcataactaa actaacgata actgcaagaa
    22501 ttgggataat aggaccaaat ggtactcgga agatcacatt agcatcggga tcgtccttcc
    22561 gtaatttcat aacggctagt gctgttggaa tgtattggaa gaagcggaaa acaacactga
    22621 gttttgctaa gttttcaaat gagccagtaa gtaggagtac aatggcaatt gctcctgaaa
    22681 ccaagatagc aacgagtggt gcgccatttt gattttgctt ggcaatagca gctggtaaga
    22741 gcccttcatc agcaatggca gcaccgtaac gtggcaccat gatggattct cccatgttaa
    22801 gacctgtaat ggaaattaaa gcaccgattg agaccatcca agcgccagca gggcctatca
    22861 ttttaacaaa ggcatcttga acaggtgcat tggtcatcat gatttgagag ccaagcatgg
    22921 caatggttcc accgataatc agcatatata agacagagac gatactgatt gatcctaaaa
    22981 gagcacgcgg tacatttttt tcaggggcac gcatttcccc agcgacaata gagagggttt
    23041 caaaaccgat aaagccatag aagatataaa cagcagtatt ggagatagcc cctaagaggt
    23101 tagtccctgg ttccaactga acaaatgggg taaagttagg aagcccgttt ttgatgaaaa
    23161 aaagggtaca ggcacaaaag gcaacgattg gaattaactt agcaatggtg gctgtgatgg
    23221 tgacaatttt agaggttttc aaaccagcga tattcatcaa agatagtaag atgattaacc
    23281 caatactaag tggaatgtgc caaccttcaa aggcagggaa agtaatgata aacattctgg
    23341 caaacccagc tgccatggct gcccaagcga agatagtaac ggtccaacca aggaagccaa
    23401 cgttaaagcc aataaaatca ccaaaagctc gtttggaata ttggaaggct ccgccgtttt
    23461 tcccaaaata accagagact tctgcaaaac aaactgccaa catgatggtc aaaatagctg
    23521 ttccaaacat aacagcgatg gatgcagggc caaggccctt gtaaatggcc ctaggtaaga
    23581 gaaaaatacc agagccgata acggcattga tcccgtaaag agtggcaccg ctcaagctaa
    23641 actttgcctg ttctctttct tgttcgttca tgtgatttga cgccataata atgctctcct
    23701 ttttgtttgc aaattgcgtt caaatataat ggataaggtc gctaggaaat gaggagatag
    23761 tcggtttagg agggtccttc ttgcacctaa cgatagtgat gagctattgg ttagcgatga
    23821 gaaataggta gccttttgtg atgatgtgta gtgatttcat tggagaagaa caggcatgag
    23881 tagtgtgatg cctatcttta tcccactaag tcttatacca taagcctgcc caaacagcta
    23941 ctggagtttg ggcggggctt gtttgattgg ggcaaacttt tagatctgaa aggctggcct
    24001 cctgctcgat taccaacaag tgattggctt caagctcttg attcgcctga ttggtcacag
    24061 aataagtcag tttgcttagg gcataaagca gttgacagtc cgctttgggt gcctcaaatg
    24121 gtgtgtcaga ccagacgggt aataattgcc cttggtattg gtcatcatac attaaattga
    24181 tgtcttggca gattccacga ctgacgattc tggtatcgcc ttcaaacgca tagggttcaa
    24241 aaggagttag ggaaatctct ttagcatgac tcaaatcact caatgtgatg ggcttgtcta
    24301 gggtcattag aatcctgtga tagccatcaa gtaaggtaaa ggttgtctgt cgtagggcca
    24361 cagttgactt tgagaggcga taagagaatt ctctcttttt gtaatctcca tcttcgggat
    24421 aaaggaacaa ttggttggtt tcaccgccag accagtcagt ttggaggtaa tcagagggcg
    24481 ctttgatagt tacctttgtc atttgttctc tccttgtttc caattagaat agtcctgtga
    24541 tgttgccctc ctcgtcaata tcaattgttt cgctggcagg tacttttgga aggccaggca
    24601 tggtcatgat agcacccgtc agagcaacga taaagcctgc tcctgcggac actttgaggt
    24661 tgctaatagt cactgtaaag tcagttggtg cgccaagttt tttggcatcg tctgagaagg
    24721 aatattgggt tttggccatg caaattgggt agttgccaaa gccgaggcgt tctaagtcag
    24781 ccagttcacg tttggctgcg ggggtcaatc taatcccctt accaccgtaa accttggtaa
    24841 cgattttagt cagtttggtt tcaatgctat catcttcctc ataaacaaag cagaattgat
    24901 tgtcttgctc ggctagtgcc acgacttttt cagctaattc acggccacca gctccgccat
    24961 ttgcccaaac atcagaaatc acaacgtcaa cgccacgttt gtcgcaggca tcatagacag
    25021 cttgtaattc agcatcggta tcaagcggga atttattgat cgccacgaca actggaagac
    25081 catagacgtc ttggatatta gccaaatgtt tgtcaaggtt aggtaaacca tccacaacgg
    25141 cctgaacatt ttcagtagcc aggtttgctt ttggcacacc cccatgcatc ttaagggcac
    25201 gtatagtggc tactaggaca acggctgctg ggcgaaggcc tgacatgcga catttgatgt
    25261 caatgaattt ttcagctcca aggtcagcgc caaatccagc ttcggtaacg gcataatcgc
    25321 catatttcaa ggctagtttg gtggctagga cactattaca gccatgggcg atattagcaa
    25381 atgggccacc gtgaatgagg gctggtgtgt gttccagggt ttgcacgaga ttaggatgaa
    25441 tcgcatcctt gagcagagcg gctagggcac caccagcttt caagtcttta gctgttacag
    25501 gttcaccttg gtagttgtaa ccgatgatga ttttttcaag gcgggctttg aggtcagaaa
    25561 tattttcgga taggcacagg attgccatga tttcagaggc gacggtaatg tcataaccat
    25621 cttcacgagg aaccccgttg accttgcctt gcaagccgtc aacaatatga cgaagctggc
    25681 ggtcattcat atccaccaca cgtttccaag tgatacgtct agaatcaatg cctaggctat
    25741 tgccatggtg aatgtggtta tcaatcaagg cagccaataa gttgttggcg acaccgatgg
    25801 catgaaagtc ccctgtaaag tgaaggttga tgtcttccat tgggacaact tgcgcgtggc
    25861 caccgccagc agctcctccc ttaaccccaa aaacaggacc aagagaaggt tcacgaaggg
    25921 caataactgc tttttttcca atggcagaaa gagcatctac caatccaacg gaagtagtgg
    25981 ttttgccttc accagctggt gttggtgaga tagcagtgac aagaatgagt ttgccatcag
    26041 gtttgtcttt taaggcgacc agttgacggg catcaatttt tgccttgtac ttgccataga
    26101 gacagagcgc ttcctcgtca atccctaatt gattggcaac cttactgata ggctccatag
    26161 tgacagagtt ggctatttca atatctgata aaaccatagt tgctccttta cttgttaaac
    26221 aatatcgaga attttcgtgt agatgtcatc tgccaagtga cagcccttgt ctaataaggc
    26281 ttgtcctttt tggcgatagt cggtaacaaa gtcttcatct ttgatgccag acagattaat
    26341 caaaacattc aaccaagcgc tttgtaaacc tgcttttaag tttaaagcag caactcctaa
    26401 gtcgctagca gcatttgtat tagacttacc gacagctgtt gcagtgatct caagtgcttc
    26461 gaccatgagg gtcatcatct caaaaggtga ttgtgcagct gttttcaagg ccttttgcat
    26521 ggcagttcgt ctagcagcct tgtcttcgtc agtctctttt ggcatatcaa aaacggcaga
    26581 aaccaggttg aaggcttcgg tatctttgtc aatggctgca agcaagctag cttgtagagc
    26641 ggtgctttta gcatggatct ctgtaataat agcttgataa tcagcatact ttttcttacc
    26701 aagggtcaat tcacagacca tcttggtcaa ggaaatgcca ttagcgccag agagagcagc
    26761 agcagaacca ccacccggag caggggcatc tgagccaagg actttagcga aatctgttaa
    26821 gcttaaatca actaaaccca tagagaacct cctaacctag caaatggttt tcaagaacct
    26881 gtttagggta gtcaaagtcc tcaacttgta agtagtattc agccacatcg attaaggcct
    26941 tggctggcgc taagccgatg acttcagaac caataacatt cacaccgtag cgacgcgctt
    27001 caaatttgat ggtttcaaaa gtacggtaaa gagaacattt ttcaaagtta accatgttca
    27061 tggagacttg ggcaatatgg cggtcttcta acatgacacc gatagcctta caatatttgt
    27121 agccaccacc tgaaccgcgg ataattttag caattttatg agcaatgtcg atattgtcag
    27181 tatccaaatt aacattgaaa gcaaccaatg gcatgcgagc accaacggca gttaccccag
    27241 cagttgggtg aatcttacga tctccgtagt caggtgccca atcctcttcc aatagttttt
    27301 ctggcatgcc ttcaaactga cctttacgga ttttagcaag gttttgacgt tctggacgtg
    27361 tggcagagtc ttcataaagg aagatcggaa tgtcaagttc acggttaatg cgttctgcga
    27421 cttgtttgga aatttcgaca cattcttgtg tagtgatgtc tttgattggg acgaacgggc
    27481 agacatcggt tgctcccata cgaggatgtt caccatgatg cttggtcatg tcaatgtttt
    27541 cagaagcgta tttcaccagt tggaaagctg cttcttgaat agactggtca tctccaacta
    27601 aggtaaacac gctccggttg tggctcgcat cagaagagta gtcgagaagg gtcactccag
    27661 gaatactttt ggccgtcgct accaaaccat cgataacagc ctgattttgg ccttctgaga
    27721 agttgggaat acattcaaca atttttgcca tgttaatctc cttttatgtc attgagttac
    27781 tcattagggg aataggttac ctactttaga taacgcttac atttttagct agcagttatt
    27841 agtgaaataa ttgatctacc gctgaagtca caaggtcatc atcagccaag tatgggatag
    27901 tgatgtgatc agtacctgca tggagacggt tgtattcaat agcggtttca atagcgtgct
    27961 catttcgggc ccagttacgg cgagctacac cgcccatagt atcccaagca atagcagatt
    28021 taataatctc atcaatgcgt tcgctgccat ctaaaacaag tccgaaacca ccattgatgg
    28081 ctttaccaat accagttccg ccaccgttgt gaagagccac taaactcata ccacgcgcag
    28141 cgttaccagc gtaacattgc accgccatat cacaggtcac attggaaccg tctttaatgt
    28201 ttgaggtttc acgaaatggt gagtcggtac cagacacgtc atggtggtca cgaccaatca
    28261 tgacaggtcc aatcttgcct ttgcggacca attcattaaa tttaagggcg acagtcacac
    28321 gaccgataca gtcttggtaa aggatccggg cttgggtacc aactacgagt tgatttttct
    28381 cagcatcacg aatccagttg tagttatcac ggtcttgata gcggcggtca ggatcgatag
    28441 cttccatggc agctttgtca gtagccacca agtcttcgtg tttaccactc aagcagaccc
    28501 aacggaaagg tccatagcca tagtcaaaaa gcataggtcc catgatatct tccacatagg
    28561 atggccagat aaatccgtct ttgtcgttac gaccattctt agaaatttct gtaataccag
    28621 agtcgtaaac agacttcata aaggcattgc cgtagtcaaa gaaataggtg ccattttcag
    28681 ttaacgtctt aatagcttca aaatgccgtg ccaaggtatc atcaaccatt tggtggaagg
    28741 tatctttatc ttctgccaag agccgggttc tttcgtcaaa gctaatacct actgggcagt
    28801 agccaccgtc ataaacgttg tgacaagagg tttggtcaga caaaagatca acatgaatct
    28861 gcttgtcatt gacgtactct aaaagatcaa cgatattacc atggtaagca atcgaagtgg
    28921 actctttagc atctatggct ttttgggcta attgcagagc ttcttcaggg ctctcagcaa
    28981 tttgggtaac ccagccttgc gaatgacggg tttcaatccg agattggtca acttcagcaa
    29041 taatggcaac tgctttagca atttcagcag ccttaccttg tgccccactc atgcctccta
    29101 aaccagatga gataaagagt ttaccggtca aatcaccatc gtcagccacc ccaagtttga
    29161 gacgaccagc atttaagagg gtattaaatg ttccatgaac gattccttgt gggccaatat
    29221 acatccaacc acctgcggtc atttgaccat agttggtgac acccatctct tcagcgattt
    29281 cccagtcttt catgttgtcg tattcaccca ccaaaaggcc attagtaata atcacacgag
    29341 gcgcttcagg tttggattgg aaaagaccaa ctggatgacc agactcaacc accaaggttt
    29401 gttcatcagt catgacttct aggtattttt taatcaaaca atattgcatc cagttggcac
    29461 aaacagaacc agtttctcca taagtgacta gttcgtaagg gtacaaggca atctcaaaac
    29521 tcaagttatt gtcaatcatg acctgcatgg ccttggcagc tgtacaattt cccttgtact
    29581 cgttaatggg tttgccgtaa atgcggtctt ttggacgcca tctgtaacca tagatgcgtc
    29641 cccttgtttt gagttcttcc aaaaattctg gaatgacttc ttcgtggaac ttcgtaggta
    29701 catagcgtag ggcatttttc aaggcgattt cggtctgagc ctgggtcaat cgaaaacctc
    29761 tgtctggtgc tcttcggata ccttctttga agacagtctt ttccggtaaa acatcgtcta
    29821 acttaacagt catagcggca gctatgtcag tttcactgta aaatgacata gaaaactcct
    29881 cctcaactcc tttttattgt cgagattagt ctaacttaga ctagtctata aaaagtcaac
    29941 cacgctttga aatgcgaaaa aagtctaaaa aaaaatcaaa acctcatttt ttgactaaaa
    30001 tttgacgctt aatcagtaca cttgaggcat gacagataaa gtcagtcaga gtttaattgt
    30061 tgttaccaaa gttgcagaga tcgcaactat tgagtgggtg aggggggact agtaatggct
    30121 attaatcagt tgacaaacga tgttaaaaaa ggaggttttt atggttgctg atgtcttatt
    30181 aacacatttt aatcaactat tttgtctaaa tgatccaggt catcctttaa ctggtcaaga
    30241 aatgaaaaaa gccactatcg ttgaagatgg ctatatagcc atcaaagacg gcctcattgt
    30301 ggctcttggt tctggtgaac cggatgctga gcttgtcggt ccacagacta ttatgcgttc
    30361 ttataaaggt aaaattgcaa cgcctggtat tattgactgt catacccatt tggtctatgg
    30421 tggcagtcgg gaacatgaat ttgccaagaa attagcaggg gtgtcttact tggatatttt
    30481 agctcaaggt ggtggtattt taagcacggt gagggccact cggtcagcca gctttgacaa
    30541 cctttatcaa aaatccaaga gattgctaga ctacatgttg cttcatggag tgactactgt
    30601 cgaagcgaaa agtggctacg gccttgattg ggaaacagaa aaacgccaac tggatgttgt
    30661 tgctgcgtta gaaaaagacc atcctattga tttggtgtct acgtttatgg cagcccatgc
    30721 gattcctgaa gaatacaaag ggaatcctaa ggcttatctc gatgtgatta tcaaggatat
    30781 gcttcctgtt gttaaagagg aaaatctggc agaattttgt gatattttct gtgaaaaaaa
    30841 tgtttttaca gctgatgagt cccgctatct gttaagcaaa gccaaagaaa tgggcttcaa
    30901 gcttcgtatt catgcagatg aaattgcgtc tatcggcgga gttgatgtcg cagcagagct
    30961 cagcgcagtt agtgcagagc atttgatgat gataacagat gatggtatcg ctaagttgat
    31021 tggtgctggt gttattggta atttgcttcc agccaccacc tttagtttaa tggaagacac
    31081 ttatgcccct gctcgcaaaa tgatcgatgc cggcatggct atcactctct caacagatag
    31141 taatccaggc agttgcccga ctgctaacat gcagtttgtg atgcaattag gttgttttat
    31201 gttacgttta acaccgattg aagtcttaaa tgctgttacc attaatgcgg cctactctgt
    31261 caatcgtcag gagagggttg gtagtttgac agttggtaaa gaagcagata ttgccatctt
    31321 tgatgcccca aatattgatt acccatttta tttctttgcc accaacttga ttcaccaagt
    31381 ctataaaaaa ggacaactca cggttgatcg aggtcgtatt ctttaaaaaa tttcaaaaag
    31441 aagctttgag cgttagctca aaacttcttt taaatatgct attttattga cgaatcaagt
    31501 agtcaaaggc accgatggca gcagtagcac cagaccccat ggaaataatg atttgtttat
    31561 aagctgaatc agtacagtct ccagcagcaa agataccagg aatattggtt gacccatgtt
    31621 tatccacaat gatttcaccg cggtcggtta ggttgacacc actatctttg agccaagctg
    31681 tgtttggaac aagaccaatt tgaacaaaga ccccttcaag gtcaaggtgt ttgtcttcac
    31741 cgctatcacg ctcagtgtag ctaagacctg taacatggtc ctctccaaca atatccttag
    31801 tagcaacatt tttgatgatg gtcatattgt tagtcttagc tgcgcggtct tgaagcactt
    31861 tgtcagcttt aagttcaggc aagaattcta aaacgtaaac gtgtttagca agaccagcca
    31921 agtcaagggc cgcttctaat ccagagttcc caccaccgat aacagcaaca tctttgcctt
    31981 caaagagagg accgtcacag tgagggcagt aagtcacccc tttattacgg aattcatctt
    32041 caccaggaac attaatgtta cgccatttgg ccccaagagc taagatagca gttttggctt
    32101 gtagaaccgc accattagct agagtaacct caatgttttc ttttttctca atagaagtag
    32161 ctagttgtgc tttgataata tcaacatcat aagacttagt atgggcctcc acttcggcca
    32221 ttaattttgg tccttcagtg taaagggtac caatcatgtt ttcgatgccg accgtttcca
    32281 tgacctgacc accaaaggtt tcagcaagta aacctgtttt tagtccttta cgagctgcat
    32341 agatagcagc gctattgccc gcagggccac caccaataac aaggacatca tagaggcctt
    32401 tatcagcaaa ggcttcctca gaaagtgggc cagcgatttg ttctaagagt tgctcaatgg
    32461 tagcgcgacc agaagtgaat tcttccccgt ctaaaaagac agtagggaca gacataatgc
    32521 cttttgcttt cacttcatct tggaacatgc cgccttctac catagtgtgt gaaatcttgt
    32581 cgttcaagac ggacataatg tttaaagctt gaacaacatc tgggcagtta tggcaggtta
    32641 agctgacata agtttcaaaa tgcaacggac ggtcaatggc cttgatgcga tcaataacat
    32701 cttgatccac ttttggagca cgtccagata cttgcaagag agcaagaata aaggacgtca
    32761 attcgtggcc taaaggaaga ccagcaaaga caaccccact accgtgacct tttttcgcaa
    32821 ccttaaagct tggttgtcgg tctaaagtga tgttttcaat agaaatacgc tcagacatag
    32881 cagctatttc ttccacaaag tctttgactt tttgagactg ctcattgtca ccaagggaaa
    32941 cttgcaaaac aagatcagct tctaacaggg tgaggtattg ggcaagttgt tctttaatat
    33001 caggacttaa tgccatagct ttcctcctaa aatttattaa attttaccaa ctaaatcaag
    33061 acttggtgtc aaagtttcag cgccttcttt ccatttagct ggacaaactt cacctggatg
    33121 tttacggacg tattgggcag cgtgaatttt atcaatcaag gtgctagcgt cacgtccaat
    33181 accatcagca ttaatttcca tcatttggat aataccatct gggtcaacga taaatgttcc
    33241 acgttgagca agtccgtctt cgccaagcac ttcaaaggct tgtgaaataa ggtgtgaagg
    33301 gtcaccaatc atagggtatg tgatagtgcc aaccacatct gagtcatcat gccaagcttt
    33361 atgaacaaaa tgagtatcag tagagacaga ataaacttct acaccaagag atttcagtgt
    33421 ttcgtattgc tcttgaaggt caccgagttc agttgggcaa acaaatgaaa agtctgctgg
    33481 gtagaaacaa aaaactgccc attttccttt aacgtcttca tttgtaacag tgatgaattt
    33541 tccatcgtga taagcttgag ctgaaaattc agcaatttct tttccaatta gagacatata
    33601 gttgtcctcc ttttttatag ttttatactt gtattataaa agaacaacaa gattatgtct
    33661 actcagaaac ccttgatttt actgggaaat cagcgattgt tttctgacaa ttcagttgtg
    33721 aaataaatca cattaattaa aatgattcta aataagctga agacgccgtt aaatcaaagg
    33781 ttaatttaat tataatctac agaaaatgta attgttatta aagcgtttac aaaaaaactt
    33841 ttttgattcc gttaaggcaa cttaatatta tggttaccta atcgctaata ttgtaaccta
    33901 ttttggcatg agatcaggga atggctgtta tattaattgg atatgtcttg tgcaaatagt
    33961 gcttgacgga tttgtggaat tagggtaaaa taatctttgt tgtggcggta tagccaagtg
    34021 gtaaggcacg gctctgcaaa agcttgatcg tcggttcaaa tccgtctacc gccttttata
    34081 tacaaaacaa gccttgatta atttcgtgaa aacgttgtta aatcaaggtt tttatttttt
    34141 gcgacaggat aaaaaaagag aaaaaaagaa ataaagtcgg tcaaaagtcg gtcaaatttc
    34201 ccgaagatgt cttttatagc ctgattatta ggtatgaaaa atactatggc caaaattggt
    34261 aaacgattgc aaataaaggt cagagttctt acccccttaa acgatatgcg ttaaaagtta
    34321 gttgaactgc cgtgcaccta acgatagaca cggtgatgtg agagcgagta gagttgaatc
    34381 tctacttgtt tatcttacca tagtcagctt tataaagtaa ccacccatta caatgcaact
    34441 atattcttaa tgtttttaag catgttattg acaagtgtta taaaatgtgt tactcttagt
    34501 gagtaaatta gtttttgagt tagtatttgt gtttcaacat ctttatttct ctctattttt
    34561 acaaatgatt tttagaaagg aagtggatgt atatggcaca aggtacagta aaatggttta
    34621 acgctgaaaa aggttttggt tttatttcaa ctgaaaacgg tcaagatgtc tttgcacatt
    34681 tttcagccat ccaaactaat ggttttaaaa cgttagaaga aggacaaaaa gtggcatttg
    34741 acgtcgaaga aggtcaacgt ggacctcaag cagtcaatat cactaaattg gcctaatctg
    34801 gttaaagtaa ctaaagagaa gcagatgttg gaaacaacaa ctgctttttt gatttctacc
    34861 agttaccttt aatgattaag agttgcgttt aaggtcttgt atttgttata ataatagtca
    34921 gttttagtca aaatgtattg acgtggtgct actaagtttt tagagtagcc cgcaagcaaa
    34981 ggagtgacag catgccaacc aaaaatactt cagacagcat tgaagagtat attaaagaat
    35041 tattagctaa gtcaggaata gcagaaatta agcggtccat gctagcagat tcgtttcaag
    35101 ttgtcccaag tcagatcaac tatgtcatta aaacccgttt tacggagagt cgaggctatg
    35161 aagtagagag taaacgaggt ggtggaggct atattcgtat tgccaaggtt catttttcag
    35221 acaaacacca tctgattggc aatttaatgg caactattga agactgtatc agtgagcaag
    35281 ttttcacgga ctcgatacaa ttgctttttg acgaacactt attgacagag cgtgagggaa
    35341 atatcatttt ggctgtggcg tctgatgatg ttttaggaac tgatggttca actattcgtg
    35401 cacgaatgct ctatcgctta ttgcaacgaa ttgacagaaa gggaagcaat taatgattat
    35461 gtattcaacg aagatgcaag acatttttag acaggcgcag ttccaagctg ctcgctttga
    35521 tagccattgc ctggaaactt ggcatgtttt gttagctatg gtagctgtag ataattcttt
    35581 agcaaatatg attttaagtg aatatgatgc ccaagtcgcc atagaagaat atgaagctgc
    35641 agctatttta gccatgggca aaacccctaa ggaacagttg tctcgtgtag acttcagacc
    35701 tcaatctaaa accttgacta acttgttagc ttttgcgcag gctattagcc aaatcactag
    35761 ggatcaagaa gtcggctctg agcatgtctt atttgctatt ttattgaatc cagatatcat
    35821 ggcgagtcgt ttgttagaaa tagcaggcta tcagataaaa gataacggca atgggcagcc
    35881 gcgattagct gacttgcgaa aagcaataga acgtcatgca ggttacagta aggaaatgat
    35941 taaggctatt cacgaactac gtaagcctaa aaaaacgaaa acacaaggga ccttttcaga
    36001 tatgatgaag ccaccaagta cagctggtga attgagtgat ttcacaagag atttgactga
    36061 aatggcaaga caaggtttgt tagaatcggt gattggacgt gaccaagaag tatctcgtat
    36121 gattcaggta ctaagtcgta aaacgaaaaa caatcctgtc ttggtaggtg atgcaggtgt
    36181 tggtaaaact gcgcttgctt atggccttgc tcaacggatt gcaaatggcg ctattcctta
    36241 tgaacttaag gagatgcgtg tcctagaatt agacatgatg agtgtggtag caggaacccg
    36301 ttttcgtggg gattttgaag agcgcatgaa tcaaatcatt gatgatattg aagctgatgg
    36361 tcagattatt ctttttgttg atgaactaca tactattatg ggttctggca gtggtattga
    36421 cagtacactt gatgcggcta acattttaaa accagcatta tcgcgcggta ctcttcatat
    36481 ggttggagca acaactcaag aagaatatca aaaacatatt gaaaaagatg cagctctttc
    36541 gcgtcgtttt gctaaaatat taattgaaga acctaataca gaagatgctt atcagatttt
    36601 gatgggccta aaattatctt atgagaccta ccataatgtc tcgatatcaa atgaggcagt
    36661 taaaacagct gtaaaaatgg cacaccgtta tttaaccagt aaaaatctcc ctgattcagc
    36721 tatcgattta ttagatgaag ctagtgctgc tgtgcaaaac atggtgaaaa aatcagctcc
    36781 tgagacttta acaccaatag accaagctct tatcaatggt gatatgaaaa aagtatctcg
    36841 cctcttagct aaagaagcaa aaggtcagat gagaaaacca acaccagtga cagaagatga
    36901 tattttggca accttgagta agttatcggg aattccactt gaaaaactga cgcaagctga
    36961 tagtaaaaaa tacctcaatt tagaaaaaga actgcataag cgtgtgattg gtcaggatgc
    37021 tgctgttacg gctatttcaa gagccattcg tcgcaatcag tcaggtattc gaacaggaaa
    37081 acgtcctatt ggatcattta tgtttcttgg cccaacagga gtaggtaaga cagaactagc
    37141 aaaggccctt gcagaagttc tctttgatga tgaagcagcg cttattcgtt ttgatatgtc
    37201 tgagtacatg gaaaaattcg cagcgtctag gcttaatgga gcacctcctg gttatgtcgg
    37261 ctatgatgaa ggaggtgaac tgacacagaa agttagaaat aaaccttatt cagtcttgct
    37321 ttttgatgaa gtggaaaaag cacatcctga tatttttaac gttctccttc aagtattaga
    37381 tgatggtata ttgactgata gtcgtgggcg taaggtcgat ttttcaaata ctattattat
    37441 catgaccagc aatcttggcg caacagccct gcgtgatgat aaaacggtcg gttttggggt
    37501 caaaggcatt caccaagacc atcaagctat ggagaaacgt attttagaag aattaagaaa
    37561 aacttaccgc ccagaattta tcaatcgtat tgatgaaaaa gtggtctttc atagtctgac
    37621 ccaagataac atgcgcgatg tggttaaaat catggtacag cctctgatta ctacattggc
    37681 agaaaaaggt attaccctta aaattcagcc tttggccttg aaacatttgt ccgaggtcgg
    37741 ctatgatgag catatggggg caagaccatt acgtcgaacg ctgcaaactg agatagaaga
    37801 taagctatca gagcttatcc tttctcgaga attgacaagt gggcatacgc taaaaattgg
    37861 attatcatat ggcaaattaa cgtttcacat agcttaattt ccatataaaa ggtggatcat
    37921 ccttgatgtc accaaaaaaa gaggttattc ttacaaaaat aacttctttt ttcttgacta
    37981 tttttgacca agtgataaaa tagaatataa agttagcact cgatgaacat gagtgctaac
    38041 tttactaaag attcttttat tggaggaaaa tttcatgtta aaaccattag gtgaccgtgt
    38101 ggtcgtaaga tttgatgatg aaaaagagca gacagtcggt ggttttgtcc ttgcaggcac
    38161 tcataaagag tcaacacgaa aagcgacagt cctcgctgtc agtgaaacgg gtgttcgcac
    38221 tattacaggt gatagcgtgc taccttcagt ttcagtgggt caagaggttt tagttgaaaa
    38281 tgggcatgat ttagaagtaa ccgtagatga tgagaaagtt tctatcatcc gtgaatctga
    38341 catcattgct attgttacaa aataaaacga caaatctcta aatagaaaga aggattgaat
    38401 atggcaaaag atattaaatt ttcagcagat gcgcgtgctg ccatggtgcg cggagttgat
    38461 atgttagcag ataccgtcaa agtaacgctt ggtcctaaag ggcgtaatgt tgttcttgaa
    38521 aaagcttttg gttctccctt aattactaat gacggggtaa ccattgctaa agagatcgaa
    38581 ttagaagatc attttgaaaa catgggagca aaattggtgt ctgaagtggc ttctaaaacc
    38641 aatgatattg ctggtgatgg gacgactact gcaacagttt tgacacaagc cattgttcat
    38701 gaaggactaa aaaatgtgac agcaggtgct aatccaattg gtatccgtcg aggcattgaa
    38761 acagcaacag caacagctgt tgaagccttg aaagccattg ctcaacctgt atctggcaag
    38821 gaagctattg ctcaggtcgc tgcagtatca tcacgctctg aaaaagttgg agagtatatc
    38881 tcagaagcta tggagcgtgt gggcaacgat ggtgtgatta ccatcgaaga atctcgaggt
    38941 atggaaacag aacttgaagt ggtcgaaggc atgcaatttg accgtggtta cctgtctcaa
    39001 tacatggtca cagacaatga aaaaatggtt gcagaccttg aaaacccatt tatcttaatc
    39061 acggataaaa aagtgtcaaa catccaagac attttgccac tacttgagga agttcttaaa
    39121 accaaccgtc cattactcat tattgcagat gatgtggatg gtgaagccct tccaaccctt
    39181 gtcttgaaca agattcgtgg tactttcaat gtggttgctg tcaaagcgcc aggatttggt
    39241 gatcgtcgta aagctatgct tgaagacatt gctatcttga caggtggtac agtgattaca
    39301 gaggatctag gacttgaatt aaaagatgct acaatgacag cccttggaca ggctgctaag
    39361 attacagttg ataaagatag cacagtaatt gttgaaggtt caggaagttc agaagctatt
    39421 gctaaccgta ttgcactgat taaatcgcaa ttagaaacaa caacttctga ctttgaccgt
    39481 gaaaaactac aagaacgttt ggcgaaatta gctggtggtg tagctgttat caaagtagga
    39541 gctccaacag agacagcttt aaaagaaatg aaacttcgca ttgaggatgc tctaaatgcc
    39601 acacgtgcag ccgttgaaga aggtatcgtt gctggtggtg gaacagcact tattacggtg
    39661 atcgaaaaag tagctgctct tgagcttgag ggcgatgacg ctactggacg taacattgtg
    39721 cttcgtgctc tagaagagcc tgtacgtcaa attgctttaa atgctgggta cgaaggctcc
    39781 gtagttattg acaagttgaa aaacagccct gcaggaacag gatttaatgc tgcaacaggt
    39841 gagtgggttg atatgattaa aacaggaatc attgaccctg tcaaagtaac acgatcagcg
    39901 cttcaaaatg cagcttctgt agctagtctt attttgacaa cagaagcagt tgttgctaat
    39961 aagcctgaac cagctgcgcc agcgccagca atgccagcag gtatggatcc aggaatgatg
    40021 ggcggcttct aagctctcta tagcattaag gattatgaaa aagaagttga gactaaaggt
    40081 ctgcttctta ctatgaaaaa agaacaaaga ttggatttcg actcatgata tcccatcttt
    40141 gtttttttag ttgtgatata aaatgccaat ctttgtggtt acagactttt aatgtattgg
    40201 aaaatatatt gtttttcttt gtcatgtgct atcatggagc caatgaaaag ttattcccat
    40261 atatgcgtgg tggaggtctg tgatgaatca ttttggagag atttttaaaa cctttagaga
    40321 atcaaaggga ttacgattaa aagatgtcgc aaaggctggt atatcaacct ctcagctgtc
    40381 gcgtttcgaa aaaggggaga cggatttaac catatcgaca tttatgctga ttttggatga
    40441 aagtaacatg tccattgatg agtttatgta tgctgtccat gattttcatc gtgacgactt
    40501 aaatgaactc ttatcaaaaa gtgagggatt tcgtaacaat tcaagatagt gatggcttga
    40561 agcagttact tcatgctcaa ctagcatcaa ctgataagaa ggaaatgttc acggatatct
    40621 tttggcatat ctagcatgtc taagtctgct atggcatgcc attttgggag tccctcacgc
    40681 gactctgtca gtagctctcc ttcaaaggat tcacatagaa agtcgtaata gacatagcac
    40741 tctttttttg tcaggcttga taaagctgga aatacccttt aattgaaggt ttaaagctgt
    40801 cagacctatt tcttctttta gttctcataa agccgcttca aagaaagatt cacagggctc
    40861 gactttacct tctggctgga cccatcctgg taagttatcg tgttgtctat ggagtcataa
    40921 aatcatatcg ttatgtttga cacacatatt gccccagtct ctaaccgttg ctatcaccag
    40981 attcaccacc ttttagtata agaaaaaagg ggtgaaagag gtttcttgtt gtttttttgg
    41041 cttaagattt ctttaagtct tatctagtat actaatgtca ttccttaata ggataagtat
    41101 ttggttgtag aagtatcgtc ttaaactaac gctatctcaa ccgattccca acttattcac
    41161 ccctcctata ccgataaggt ctcacaaagt gtgagacctt attttttgac ttcagctcta
    41221 gttatggaga aacttgttga tacaagcttt tttgggttat gggttatggt attatgatct
    41281 attcaagcct ttcttgtttt ggggtttttc ttcaaccaac ttggcttcaa tagcttttaa
    41341 gcgtttgaag gttttttcag cgcgatctag tgaaatctta tcagcttttt cttgagcagc
    41401 tttggggtca ctgacgacta agtcactgat ttctttggtg cttttgtctt gttcggctat
    41461 ccaggttttt tccaagcctt tcatctcatc aatcacttta gttccaaatg gttttggatg
    41521 agcagccacg agatcattaa tgtgtgacac cgtccaatac catgacttat cgttatattg
    41581 ggtgcttttt tcttgatagg cttcataggt tctactgata ttgccaaggt atggcaagta
    41641 aggggcatta cgaggactgc caatagatag ccacatgaca ccaccaccca attcagcagg
    41701 aatgtctttt ttcagttgga aaatatgagc ttccatgaca ttaggatttg agatggggta
    41761 agcataacct ttaacagctt ttttggattt tggtttaccc ttaccgtcta atgccatctg
    41821 atcaagtggt ttaagatcca gcccctcaaa acgattacgt tgcagtttca tcgcatcttc
    41881 tagactaaag gtcttatcag tgctttggag caactcataa ttgctgtctt tataagttac
    41941 ttttgaatca gggtcaagtg atttaatccc tgagaaagag cgagagcggt tggcatcgtt
    42001 tagtggtgga ttgtaagatt tggcaatatg aaatttacca tcaacttcgg tataagattt
    42061 ggctttttta gtgacttttt ccacatcttc tgaggcaatc gtattttcct tatcgttgaa
    42121 atcgacatgt ccgagataga aggtatttgg aaaaacagcg tatttatcat ctgggaattt
    42181 gatggcaacg tattggtgac cagagagaat ttccatgtac cagatgccat ctttgtcagc
    42241 caaggtaaca atatttcctt cagcagctcc tttttcagtg actattttag caatcaatgc
    42301 gacaccttca cgggctgttt tgacacttgg taaaatgaca ctggccatag atgattccgc
    42361 taagccattt ttgacgtaag gatcaatttt ttggatggca tcattagctg aagctgatac
    42421 tgttgcagac atagagacgc caaactcatt aaaaccagct tcgtcataaa cgcctttatt
    42481 aggtgttaca tcaggaatcg cactatagcg ataagaatgc tcaggcaatg ggtactcaaa
    42541 accattagag gggtctttcc atttttcgcc agcaggattg tcctttgcta gacgtacaat
    42601 gaagttttta ttatgatgtg gctcaagatc ttctgttcga ccataaagaa gagaaccgtc
    42661 tttggttaaa tctttgccaa taataaaacc agtacaagca taactaacac tttgaagcga
    42721 aaaggctgtc agaatactta aaactccaag tgatattttt ttgtttatca tgcgaatcct
    42781 cctagtaaac gataacgctt tcacagacat tataaaatgc tctttttaaa aattcaagtt
    42841 ttttatgaag tgttttgaaa ttgttagcta tctaaatatt atgagaattc tctcttgaat
    42901 tttggcttaa cggcccctaa aaacgtatag gcagtgaact atctaggttt cttcgaaaat
    42961 ccattagcca tttatgatat aatagtaaag aaaaacatag aaagttgggg tctgttgatg
    43021 accaaaccat ttcatcataa gaaactaaaa caaataacta taatagctgc aactagcctt
    43081 tttttattcc tgatcggtgg tgccttttac tactctaaaa atcattgtat taatgcctat
    43141 cttaaagctc gatcagccca atcaggacca gtttttgaaa atatcaaagc atatctagtc
    43201 tgggatgata ctaatgagca gatcacaaat gacgaggcga tgtatactaa gtttagaagg
    43261 tatagtcaaa aagaattgag gcaaaaaaag caggatttaa aagccgctag tcaagatagt
    43321 gccgttcaag taaagtctgt tggtcgtcgt ttttggattt ttcctgatta tcggattgcc
    43381 ataaaaccca tggatttaac gataaaaacg aatgtgcctc aagcagacgt tcttttaaat
    43441 caaaaaaaag ttgctgtttc tgattcagaa cagttctcag taaagcttga tcggctacca
    43501 acggcagaat ataccgcaag tatcagaggc aaacacaacg ggcgaaacat taaagtcaac
    43561 aaatcatatg atggtgataa tcccgtgcta gatttgagtg tgtcttttag aactttttta
    43621 gtaacaagca atgctaagca aggagatctt tacttcgatg ataaccatat tggcacatta
    43681 aaagatggtc aattacaagt agaagattac cctgttacag aaaacgcaca agcttatatg
    43741 aaaacaacat tcccagatgg tgagttaaga tcacaaaaat atgctctagc tgatgttgaa
    43801 gaaggagcaa ccctggagat tttagttaca gatcttttag aagaggacaa agcaggggag
    43861 ctattagtat cagcttttga tcagctgatg cactacctca gtacaggtca agactcatct
    43921 aacttacgta gcgtctttga ggcagggtct agcaacgcat tttatagggg attgaaagag
    43981 tccataaaag cgaaatttca gacagataca agaaaagcca gccgtctcaa tattccatct
    44041 atccttttga caacaatgac tcaagtgggt aaaacaactt acgtgcttga tttcacagct
    44101 acctacgaat ttttgtatga caactcaaca gatcctgagc agcatacctc tggacatatt
    44161 aatcaagact tgactggaaa agtgactgta aaaaaagttg gacagcatta ccttatcagc
    44221 cagtccggct ctaaaaatat tactgttgtt aaagaggaca atcaactcaa agcgccatct
    44281 gtctttcctg agtctatttt gggaacgtgg acaggccaag ccaatggttt aagcatccat
    44341 atgtctctag catcagatgg aacaattacg actaaagttg aagatcgaaa aggcaaccgt
    44401 tctaaagaaa ctcggacagc taaaattagt aaagttgaag acaaaggcaa tggtttttat
    44461 ctttatacgc cagatcctgg aagtgacata agcgccttag ctccagaagg aggattgggg
    44521 ggtgcaaatg tcaaatatgc ttatggtttc aaaatatctg gtaaaacagc ctctccagtg
    44581 gtgtggcagg cagcattaac acatgaattt gattatacca agccactttc gggagtaact
    44641 ttgcaaaagc aaccataaag gatgcaaagc cacaaagaac agtagaaaca atgttatcac
    44701 tcaaatacag tgctatgaaa acagataaac tcaaaaggct aggagattat ttcctagcct
    44761 tttgagcgtt taagactgcg tcaaatgtag ctgatttagc ttgcacgcaa atgtcttctt
    44821 gggttagagg gtgttttaaa cgtaattgat aagcatggag catcagtcga gcacaatctt
    44881 tttttccatt agaatacaaa ggatcaccaa ataaaacatg accttgatgg gctaaatgga
    44941 ctcgaatttg atgagtccgt ccagtttgta attgacaatt gagtaagctt gccgttttgt
    45001 ggaagttttt gactagagtt acctcagtaa tagctttctt gccatttatc ggatcgacaa
    45061 cacgttttct gcggtcatgt cgatggcgac caattggatg atggtaagtt accctaggac
    45121 tgtctaagga tccgtgaact agtgccaaat attctcgatg gatgtctctt ttttctaaaa
    45181 gacgattaag gattggtaag ataaaaggtg ttttagcaaa taaaatggcc ccacttgttt
    45241 ccttatccag acgatgaacc acatagcagg tctgtcccgt gtaagcagac acgtggttga
    45301 gcagcgccaa ctctgttgga tcattgccgt gtgttttcat cccttcaggt ttgttaacaa
    45361 taatgatgtg ctcatcttca taaaggcagg ttactttttc ggcctgcccc atcacgataa
    45421 tcttttcagg ataatcttcg tgatcgaaaa aaagtttgac ctgatcaccg tatttaacac
    45481 aactttgcca gttaacagga tgaccattaa taagaacatg ttttttagtt ctcagaaaat
    45541 gcctaatttt acggggaatt aacaattgtt cctctaataa tgctttgacc gtcaattctt
    45601 caaagggatt tttcagtgtg acagtaaacc cctttgatgg tttatttgtt gtcttcattg
    45661 agccagttat ggtatttttc aacaaggttg atagccattt gggtggcaag agcagcagag
    45721 aaagcttcag taccttcacg ctcgtcatta gctacctcca agcgagtctc atcgtaaatg
    45781 gcttttagac tttctgcgga taaggtttct ttaaaagctt ggaatttatt cattggttaa
    45841 acccgtcctt ctatgtttaa tgtgagctcc tgattaatgc aaggaattca atgctattat
    45901 agcatgtttc taatcaaaac gtgccactca aaaaggtaaa aacgttattt tgctaatcca
    45961 tctaataaga aagtgtctta aataagacct cttgatagag tttgtaacag aaatgatatg
    46021 aaactaggtg ctaacgcaga aatagaaaac aactaacgtg ttgcgaagtt ggaacagtca
    46081 taaaaaaata gcaagataaa acttatcaac acacagttac ctaagacgat gagtcaaaca
    46141 ggtatgttct ttcaaaacaa ccccatgagg ttgaacgacg tatcggaaaa atacggatta
    46201 gtttggcctt atgataggtc cttctctatc gtaaaagaag tcaaccaaag gtttttaata
    46261 taaaatatta agaaaaattg ttataattat gataaaaaca taatcgtaaa atagaggaaa
    46321 aacaatgaaa acaattagac gttatgatgt taacgaagat agagggcata caggccttgt
    46381 agaagcagga gatttctatt atttaaacta ttgtgtcggc aatgttggac aagacattga
    46441 aagtcagatt aatggagcct ttgacgagat ggagcgccga ttagcattgg tggggctcac
    46501 attagatgca gttgttcaaa tggattgtct atttcgagac gtttggaaca tacctgtgat
    46561 ggaaaaaatg attaaagagc gctttaatgg cagatatcct gctcgaaaat cgattcaaac
    46621 ggaatttgca catcacggtg gacctcaagg actactcttt caagtggatg gtgtggctta
    46681 ttcaaaacat atttcgatga cctaatcaag gttactttaa gatcatagtg gtataataac
    46741 gttatgagat ttctagaact tttacaaaag aaattttttc ctaaagcata tcaggaaaaa
    46801 caattcttaa tgcatcaaaa aacgcgttta acgccacaac acaatcaaaa gcagtattcg
    46861 ccaaatgcca atcatttgga ctcatcagct accaaaaact cagaacaaga ccctgcaata
    46921 gctctgcaac gcagtagagc ctatgaagga agccctaaaa gtcggcccgc ttggttgcaa
    46981 aagttggaag ctgttttgcc gtctcctcaa catccaattc ggcgtttttg gcgccgctat
    47041 cacatcggaa aactgctaat gattctgatt ggaactcttg tcttactctt aggatcatac
    47101 ttgttttact tatcaaaaac agctaaagta tctgatttac aagatgcctt gaaggctaca
    47161 actgttattt atgatcacaa aggagagtat gcaggcagtt tatctggtca aaaagggagt
    47221 tatgttgagc tcaacgctat ttcagatgat cttgagaatg ctgttattgc cactgaggat
    47281 aggacttttt acagtaatag cggtattaat cttaaacgct tcttattggc ggtagttacg
    47341 gcgggccgct ttggaggtgg ctcaacgatt acacagcaac tggctaaaaa tgcttatctc
    47401 tcacaagatc agacaattaa acgaaaggcc cgagagtttt ttttggcgtt agagttgacc
    47461 aaaaaataca gtaaaaaaga tattcttact atgtacctta acaactccta ctttggaaat
    47521 ggagtttggg gagttgaaga tgccagtcaa aaatattttg gaaccacagc tgctaactta
    47581 acactggatg aagctgccac attagcaggt atgctcaaag gacctgaaat atataaccct
    47641 taccattctc taaaaaatgc tactcaccgt agagatactg ttttaggagc gatggttgat
    47701 gccaaaaaga ttacccaaac aaaagctcaa caagctagag cagtagggct aaaaaatcgc
    47761 ttagctgata cttatgttgg taagacagat gactacaaat acccatccta ctttgatgct
    47821 gttattagtg aagcaatagc aacttatggt ctttcagaaa aagacattgt taataatgga
    47881 tacaaagttt acaccgagct agatcaaaat taccaaactg gcatgcagac gacttttaac
    47941 aacgatgaac tatttcctgt ttcagcttat gacggtagct ctgctcaagc agctagtgtt
    48001 gctttagatc ctaaaacagg aggtgttaga ggtctgattg gtcgtgtgaa tagtagtgaa
    48061 aatccgactt tcagaagttt taactatgcg actcaagcaa aacgtagtcc cgcatcaaca
    48121 atcaaaccac tcgtggttta cgcgccagcc gttgcttcag gatggtcaat tgaaaaagaa
    48181 ctaccaaata ccgttcaaga ttttgatggc tatcagccac ataattatgg aaattatgaa
    48241 tcagaagatg ttcctatgta tcaagcatta gcaaactctt ataatattcc agcagtttct
    48301 acattgaacg atatcggaat cgataaagcc tttacctatg gtaaaacatt tgggttagat
    48361 atgagctctg ccaaaaaaga gttgggggta gctttaggtg gcagcgtgac aaccaatcca
    48421 ttggagatgg ctcaggcata tgctgccttt gccaataatg gagtaatcca tcctgcgcac
    48481 ttgattaacc ggattgaaaa tgccaggggt gaagtgctta aaacctttac tgataaggct
    48541 aaacgtgttg tcagccagtc tgttgcagat aagatgacag ccatgatgct aggtaccttt
    48601 tcaaatggaa cagcagtcaa tgctaacgta tatggctata cactagctgg taaaacagga
    48661 acgacagaaa ccaacttcaa tcccgactta gcaggcgatc agtgggttat tggttatacg
    48721 ccagatgttg ttattagtca atgggtagga tttaatcaga ccgatgaaaa tcattatcta
    48781 acggattcaa gtgcaggcac ggcttcagct atttttagca ctcaggcatc ttacattttg
    48841 ccttatacca agggcagcca atttcatgta gataatgcct acgctcaaaa tggtatttca
    48901 gctgtttatg gagtcaatga aacaggtaat caatcaggag ttgacactca atctattatt
    48961 gatggtttaa gaaaatcagc acaagaagct tcgcaatcac tatcaaaagc agtcgatcag
    49021 tcagggttac gtgataaagc ccaatctatt tggaaagaga ttgttgacta ttttagatag
    49081 cttgcagtat tttagagatc gtgttaaaat aatagttacg gaggcgttat ggcacagaaa
    49141 aaagcgagtc tagcgtgtgt cgaatgtggc agtcgtaact actctatcgg agtgagtagc
    49201 acgcctaaac caacacgtct agaagtaaat aaattttgca aatactgcaa aacatatacc
    49261 ttacacaaag aaacacgtta aaaaggagag cctgatggga ttcattagcg ggacttttaa
    49321 agtcttaaaa gacacaacat ggccaaatcg taaacaacgt tggaaagatt ttatctcagt
    49381 tcttgagtac acagctttct ttacggtgat tatctacatc tttgaccagc tcttagcaaa
    49441 gagtgtatta gcactgatta atttatttta ggtcaagatt ggtagaaatg ataagcttgc
    49501 ttggaaaagc ctaggctggg agtattccca gccttttttg tagtatcaat aatctaatta
    49561 gtaagctttt ttaaagccca tactggtttg agctattcaa aagcataaag gtaaaggcaa
    49621 tgtagcaacg atctccaaac caagttcact aaattagctt gtaacagagt caacgcataa
    49681 acagcctggc ctcacttgct tagcacatta aaaatagcaa tgaatattat ggttgaggaa
    49741 aacagcctga tagtctgcta aatcttcggg atgataagcc ttgacctcag ccatctcata
    49801 ctccctacct agaagtttgt acttgttttc tccaaattga tggaaaggta acagttgcac
    49861 ttggtcaatc tcaagctgat taaagagctc tgagaaggct ttagcatcgt ctagtgaatc
    49921 attaaattgg ggaataacag ggatgcgcaa gacgatttct tttccagctt gaaaggcata
    49981 atgaatattt ttgataatca agtcattacg aaccccagtc actttttggt gtctgagctg
    50041 attataatgt ttcaaatcgg tatagataaa gtctacgtag tcaataaggg tgacaaattg
    50101 ctcgtgtttg gcaaaggcgg tggtttcaat tgcggtgtga agacctgctg ctttggctgc
    50161 ttttaacagg gctaaggcaa agtcaaattg ggcaaagatt tcaccccctg acagggtcat
    50221 gcccccacct gactcttcgt agaaatcaag gtctttaagg acttcttcga tcacctcgtc
    50281 cacggttttt tcctctccaa ctattttagt attaagccca tcacttgtta acatctgctc
    50341 aggtgctttt tgttgggatt cgggattggc acaccaagga catctcaagg gacaaccttt
    50401 taaaaaaacg gttgtccgaa taccagggcc gtcatgaata ctaaaatgtt ggatgttgaa
    50461 aacaataccg cgatcagtca taagtgtgaa ttcctttcgt tgtaaaacca tcataccaga
    50521 taaacgtaaa taaatcaatt acgaatgaaa gtaaatatct cttttctttt aagaaaataa
    50581 ctggtacaat gaaagggggg tgaatgatga atcgattaga acgtattatt caactggttt
    50641 cccagaagaa aaaaatagat gttaacagtt tatcagagca attagaagtt tcgaaagtaa
    50701 ccattcgaaa agaccttgat aaacttgaaa gtaagggctt gttgcgacgg gaacacggct
    50761 atgcggtgct aaacagtggc gatgacctca atgtacgctt gtcctacaat tacaacataa
    50821 agcgacggat tgcagaaaaa gcagctgaat tggtgcaaga caacgatacc attatgattg
    50881 aatctggctc aacctgtgcc cttttggcag aagtgctttg ccagaccaaa cgaaatatta
    50941 aaatcattac caattcgtgc ttcattgcca actacattcg tcaatatagt agctgtcaaa
    51001 tcattttact gggcggttat taccagccca attcagaggt gactgttggt cctttattga
    51061 aagaaatgat atcccttttt catgtcaatc gtgtctttgt tggaacagac ggtttcaaca
    51121 aagatcttgg ctttatgggc aaggacatga tgcgctcaga aggggtgcgt tacatggcag
    51181 atgctgctga ggaagtggtg attttgaccg attccagcaa gttttcaaaa accagtctgg
    51241 tacaccagtt gtcattagct gatgtcaatc gcgtcattac agaccaagca ctagacaagc
    51301 aaacacaaga gttattaagt tctggtggtc ttgttcttga ttttgtttct tagaaaatag
    51361 gtggctagat gaaagaagaa cgcagacggc tccttgccaa ggtggcttac ttgcattatg
    51421 ttcaaggcaa gagccaaacc ttgatttcaa aggaaatgaa tatctaccga acaacggttt
    51481 gtcggatgtt agcaaaagcc aaggaagaag gcattgttcg gattgaaatt gccgactatg
    51541 atgctgattt atttgcttta gaagaatacg tgcgaaagca gtacggcttg gaaaaactag
    51601 accttgtgcc caaccaggtt gaagataccc caatggatac cttgacaaat gttgctaaaa
    51661 cagcagctga ggtttttaga catgttgtta aagatggcga taagatcggt ctgtcctggg
    51721 gagcgactct ttcttgcttg atggatgagt taaatccaaa agcgatgaaa gatgtgttta
    51781 tttatccttt ggcaggagga cctagccata ttaatgccaa ataccatgtc aataccctgg
    51841 tttaccgctt ggctcgcatc tttcatggta atagcgcttt tatgaacgct atggtcattc
    51901 aagaagacaa acacttggct aagggaatct tacaatctaa atactttaac gatattttaa
    51961 caagctggga ccagttagat cttgccttag taggaattgg tggggagcct aatagccttg
    52021 agcagagtca atggcgtgat ttattaacca gctcagatca tgaccaactc aaatatgaaa
    52081 aagccgttgg tgaagtttgt tgtcgtttct ttgatcaggc aggtcagcca gtttacactg
    52141 gcctacagga tcgaactatt ggtatttcgc tagaacagtt gaggcgtgtc cctaaaacaa
    52201 tggctgttgc aacaggtaaa cacaaggcta aggcaatatt ggcagcctta aaagcaggtt
    52261 ttatcaatta tctagtgacg gataaagaga ccatgctagc tgttttagcg ttagatgaag
    52321 atattgatct taataacgtt cttctttaat agaaattcaa agatactatg atatttatag
    52381 aggcccaaga ccaaacggtc agggcttttt tgtttgatgt gttgataaaa cagagattgc
    52441 tcaaatgtgc atttaatgta ttggcacaaa agtgcattaa acaaaatata gcacaaaata
    52501 tattgaccct aaaaacaaaa atgatacaat aaacttacca acgaaatgta aacgattaca
    52561 aacataataa catcaccttc gaaaaaaaag actcattgtc gatacatcac atgaaaaaga
    52621 aaaaaaggag acaccaatgc aagttattgt accagaccaa atcattatgg gcttaatttt
    52681 aaatgcaggc gatgccaaac aacatatcta ccaagctctg aaatgtgcta aagaggacga
    52741 ttatgccact agtgaaaaag aaatggcctt agcagatgat gccttattag aagcccacaa
    52801 tttacagacc cagtttcttg cccaagaagc tagtggtaat aagtcagaga ttacagctct
    52861 ttttgttcac tcacaggatc acttgatgac gaccattaca gaaattaatc tcatcaaaga
    52921 gattattgac cttcgtaaag aattggcaac taaataagtt ttcatgacta aaaaggagat
    52981 tgacatgatt aaaattggat tgttttgtgc agcaggattt tcaaccggca tgttggtgaa
    53041 caacatgaaa gtagctgctg aaaaaaaagg cattgactgt cagattgaag cctatgctca
    53101 agggaaatta gcggactatg caccattact tgatgtggca cttttaggcc cacaggttgc
    53161 ctatactctg gataaatcag aagctatttg taaagacaat gatatcccta ttgcagttat
    53221 cccaatggct gattatggaa tgttagacgg caacaaggtg cttgatttag cccttagcct
    53281 tgttaaagaa taggagtcaa tcatggcaaa aatgaatatg caaaacatca tcaagccaat
    53341 catgacattt gtgaatatgc gcggcatcat tgccttaaaa gacggtatgt tagccatttt
    53401 acctttgacc gttgtgggga gtcttttcct gattgctggg cagattcctt tccaaggggt
    53461 taatgatgcc attgctagtg tgtttggtgc tgactggaca gaacccttta tgcaggttta
    53521 ccatggaaca tttgctatta tggggttgat ttcctgtttt gcaattggct attcttatgc
    53581 taaaaactca gatgttgaac ctctcccttc aggggttcta tccttgtcag ccttctttat
    53641 tttattgaga tcatcttatg ttccagcaga aggtgaagct attggagatg ctatcagtaa
    53701 ggtttggttt ggtggtcaag ggattattgg tgctattgtg attggtttaa cggtcggagc
    53761 tgtttacaca acatttatcc gtcgtcatat cgtcattaaa atgccagatc aagtgccaca
    53821 agccatcgcc aaacagtttg aagccatgat tccagccttt gtgatcttta ccttgtcaat
    53881 gcttgtgtat attattgcca agtcagtaac aggtggtggc acctttattg aaatgattta
    53941 tgatgtcatt caggtaccgc tgcaagggtt aacgggttct ctttatggcg cactaggtat
    54001 tgccttcttt atttcctttc tttggtggtt tggcgtgcat gggcaatctg tggtcaatgg
    54061 aattgtcact gctcttctct tatcaaatct agatgccaat aaggccttga tggcagcagg
    54121 tgagttatcc ctagacaagg gtgcccatat tgtaacccaa caatttttag actctttctt
    54181 gattctatca ggttctggca ttacctttgg tttagtggta gctatgatct ttgcggctaa
    54241 atccaaacaa tacaaggctt taggtaaggt tgcagccttc ccagccctct ttaatgttaa
    54301 tgaaccagtt gtctttggtt ttccaattgt gatgaatcca gtcatgttct tgccatttat
    54361 cttggttcct gtcttggcag ctctcacagt ttatggcgcc attgctattg gtttcatgca
    54421 gccctttgca ggagtgaccc ttccgtggtc aacaccagcc attatctcag ggttcatggt
    54481 tggtggctgg caaggagcta ttgtgcaaat tcttatactc atcatgtcaa cgttggtgta
    54541 cttcccattc tttaaaatcc aagataatat ggcttatcaa aatgaacaag ctagtgaaga
    54601 gtcatgattt agcgtccaca caaattcttg agaccatctc cagttagagg tggtttttta
    54661 gaaaaagaag aacgatttat caggcctata ttgcacaata gtgcaaaata aagtgactat
    54721 ctcttcttat ggttaaggat acgcaaaagt cgcaatatat gataaaatag ttacgaaaga
    54781 aacatttata atttcgtaag aaagaaggag accccgcatg attgaaacaa aaagccctta
    54841 ttttggacat ttaacagaca gaatggctca ctatcgtgaa gctgtgttag acaagaaacc
    54901 ttatattgat gctgagcgtg ctattttagc cactgaagcc tatcaaaaac accaaaataa
    54961 gccagcaaac ctcaaacgcg cttacatgct tcaaactatt ttggaaaaca tgaccatcta
    55021 tattgaggat gaaagcctca ttgctggtaa ccaagcctct tcgaacaagg atgcgccgat
    55081 tttcccagaa tacactctgg aatttgtcct caacgaactt gatctttttg aaaaacgaga
    55141 tggggatgtc ttctacatta ccgaagaaac caagcaacag ctccgagaca ttgccccatt
    55201 ctgggaaaac aataacttac gtgctcgctg cggggtcttg ctaccagaag aagtgcaagt
    55261 gtacatggaa acaggtttct ttggcatgga aggcaagatg aactctgggg atgcccattt
    55321 agcggttaat taccaaaaac tcttggagca cggcttaaag ggatttgaag aacgggcacg
    55381 agctgccaaa gctgcgcttg atttgaccat tcctgaaaac attgacaaat accattttta
    55441 cgactctgtt tttattgtca ttgatgcagt caaaacttat gccaaacgct atgccaagtt
    55501 ggctagagaa ctagcaaaaa cggctaaacc agaacgtcaa gcagaattgt tagacatcgc
    55561 gcgcatttgt gataaagtgc cttacgaacc agcgaaaacc tttgcagagg ctgtgcaatc
    55621 tgtctggttc attcagtgta ttttgcaaat tgaatcaaat gggcattccc tatcttatgg
    55681 ccgctttgac cagtacatgt atccttatgt caaagctgat ttggaggctg gccgtgaaac
    55741 agaagacaca attgttgagc gtttgaccaa cctctggatt aagaccttga ccattaataa
    55801 agtacggagc caagcccaca ccttctcatc agcagggagt ccactttacc aaaacgtgac
    55861 gatcggcggt caaacacgcg ataaaaaaga tgcggtcaac ccactgtctt accttgttct
    55921 aaggagtgtg gctcaaacca aattgccaca acctaacttg actgttcgtt accataaagg
    55981 cctagacaat acctttatga acgagtgtat cgaggtcatg aaacttggtt ttggtatgcc
    56041 agccatgaac aacgatgaaa tcatcattcc atctttcatc aaaaaaggtg tctcagaaga
    56101 agacgcctat gattactcag ccattggctg tgtggaaaca gcagttcctg ggaaatgggg
    56161 ctaccgttgc acggggatga gttacatcaa cttccctaaa attttgctga ttaccatgaa
    56221 tgacgggatt gacccagcat caggcaagcg ctttgcaaaa ggacacggtc acttcaaaga
    56281 catgacatct tatgaagaat taaaagcagc atgggatgcc accctgcgcg aaattacccg
    56341 catgagcgtc attgttgaaa atgccatcga cctaggtctt gaacgtgaag taccagatat
    56401 tctttgctca gccttgacag atgattgtat cggtcgtggc aagaccttaa aagaaggcgg
    56461 agctgtttac gactatatct caggccttca agtcggtatt gccaacctat cagactccct
    56521 agcagcgctg aaaaaattag tatttgaaga aggtcgatta actccagaag aactctggca
    56581 cgcccttgaa agtgactttg ctggagagcg cggagaagag atccgacaaa tgctcatcaa
    56641 tgacgcacca aaatatggta atgatgatga ctacgcagat agtttagttg tcgaagctta
    56701 cgacacctac attgatgaaa ttgccaaata cccaaatacc cggtatggcc gtggcccaat
    56761 cggaggtatc cgctattctg ggacatcatc aatctcagcc aatgtcggac aaggaaaagg
    56821 aaccttggcg acaccagacg gtcgccatgc aggcacacca ctagcagaag gctgttcacc
    56881 agaacacagc atggacaaaa aaggcccgac ttcagtattg aaatctgttg ctaaattacc
    56941 aacagatgag attgtcggag gagtgctact caaccaaaaa gttaacccac aaaccctggc
    57001 caaagaagaa gacaaactga aattaatggc cttacttcgt accttcttta atcgcctaca
    57061 cggttatcat atccagtaca atgtcgtgtc acgtgaaacc ttgattgatg cccaaaaaca
    57121 tcctgaaaag caccgtgacc tcattgtccg tgttgccggc tactcagctt tcttcaatgt
    57181 gctctcaaaa gcaacccaag atgacattat tgaacgtaca gaacacacac tttaggagaa
    57241 aataagtatg gaatacatgt tagacactct agatttagaa gctattaaaa aatggcatca
    57301 cattttgcct ctcgcaggtg tgacctcaaa cccttctatt gccaaaaaag aaggtgacat
    57361 tgatttcttt gaacggattc gagaagttcg tgccattatc ggtgacaagg cttctattca
    57421 tgttcaagtg atcgcccaag attacgaagg catcttaaaa gatgctgctg aaatccgcag
    57481 gcaatgtgga gatagtgtgt atgtcaaagt acctgtaacc acagaaggcc ttgcagccat
    57541 taaaaccttg aaagccgaag gctatcacat cacagcaacc gctatttata cgaccttcca
    57601 aggactatta gccattgaag ctggtgctga ttatttggct ccttactata accgtatgga
    57661 aaatctcaat attgatccag aagctgtcat aggacaatta gcagaagcta ttaaccgcga
    57721 gaatgctgat agtaagattt tggcagcaag ctttaaaaat gtggctcagg tcaataaatc
    57781 ctttgctctg ggagcacaag ccatcactgc tggcccagat gtgtttgaag caggatttgc
    57841 catgccatct attcaaaaag cggttgacga ttttggcaag gattgggaag ccattcatca
    57901 ccgcaagagc atttaagaaa tagcatcagt agtaatcaca tcattaggaa tcacagataa
    57961 aggagttcaa cgatgaaagt atttgcaagt ccatctcgtt acattcaagg taaaaatgcc
    58021 ttgtttacca atgctaaaac cctaaaacaa ctaggaaata gccccatttt actgtgtgat
    58081 gacgtggtgt atggcatcgt cggagaaaga tttgaaagct atttgataga taatggcatg
    58141 actcctgttc acgtagcctt taatggcgaa gcctcagata atgaaatcag ccgcgtggtt
    58201 gccattgcca aggaaaatgg caatgatgtt attattggac ttggtggtgg aaaaaccatt
    58261 gacagcgcta aggctatcgc tgacttactt gctgttccgg tgattattgc cccaaccatt
    58321 gcttcaacag atgccccaac ctcagcctta tcggttattt ataccgatga aggagccttt
    58381 gaaaaataca ttttctattc aaaaaatcca gatcttgttt tggttgacac acaagtaatt
    58441 tgtcaggcac caaaacgctt attggcgtca gggattgcag atgggttagc aacatgggta
    58501 gaggcgcgtg ctgttatgca aaaaaatgga gacaccatgg caggtggcaa tcaaaccttg
    58561 gcaggagttg ccattgccaa agcctgtgag cagaccttgt ttgcagatgg cctaaaagcg
    58621 atggctagct gtgacagaca agtcgtgacc ccggccttgg aaaatgtcat tgaagccaat
    58681 acacttctta gtgggcttgg ttttgaaagt gcgggtctgg ctgctgcaca tgccattcac
    58741 aatggcttta cagcgctgac aggtgctatt catcacctta cccatggtga aaaagtcgcc
    58801 tatggcacgt tgacccaact tttcttggaa aatcgttcgc gtgaagaaat tgaccgctac
    58861 atcgactttt accaagctat tggcatgcca acaaccctca aagaaatgca tttagacacg
    58921 gctactcaag aggatttctt aaaaattggc cgtcaagcaa ccatggcagg cgaaaccatc
    58981 catcaaatgc catttgttat cagtccagaa gatgtagcag cggctttggt tgctgttgat
    59041 gcctatgtta cgagtcgata ggctaaccaa aaagagattt cttatttgcc agataacctc
    59101 aaaaaaacaa gtctagggag tcatttccta gacttgtttt acttattgtt ggttggtaat
    59161 agcactttta gcagggtcat tgtgggcaat acggctggca gctgcagccg caatggcgct
    59221 aacaatatca tctaaaaagg tatggcagga gttgccatct ttgtggttga gtttgtcaac
    59281 aatccccggt tttgtcttgt ccagataccc ataattggta aaaccgatag acccatagag
    59341 gttaacaatg gataaggcca ggatttcatc aatcccatac agcccttggt cggttttcaa
    59401 gatactaagg agtggctcag acagctggtt ggcttcagcc aatttatcca attctacccc
    59461 agtgataata gcattttgca cttctcgttt tgcaagcacc gcttcaacgc tctctaaaca
    59521 ttctgccatg gtcaaattgg gaatataatc attctgcaaa aaaagcacca gctctgcaat
    59581 agcttctaaa gaaacccctc gttcagccag taattgataa gaaacttctc gtaagtgatg
    59641 gtcagttgac atgtgagacc tccttttttt ctttattgta acataaaaga ttttaagaaa
    59701 cctctacaac agctaaagag gggatagttc ttttttaaga gatgttgact actgagaccc
    59761 cctacttggt aaaagtcaaa gaaacgatag taaagtaacc atcttaccta ggcctaaata
    59821 caatcaccac ccctaggtga cacacagcac ctatttcaaa aatgatatta gtatggcaaa
    59881 acaaaaagac aacgccttct tttttctcct tattatctcg tttaattttc atatttttta
    59941 aaaaaactat tgataaacta gttaagtaag cgtatactat ggttagtgag cgaaattaga
    60001 aaagaggaca agcatatgaa tctacttgga tcaagacggg ttttttctaa aaaatgtcgg
    60061 ctagtgaaat tttcaatggt agctcttgta tcagccacaa tggccgtaac aacagtcaca
    60121 cttgaaaata ctgcactggc acgacaaaca caggtctcaa atgatgttgt tctaaatgat
    60181 ggcgcaagca agtacctaaa cgaagcatta gcttggacat tcaatgacag tcctaactat
    60241 tacaaaactt taggtactag tcagattact ccagcactct ttcctaaagc aggagatatt
    60301 ctctatagca aattagatga gttaggaagg acgcgtactg ctagaggtac attgacttat
    60361 gccaatgttg aaggtagcta cggtgttaga caatctttcg gtaaaaatca aaaccccgca
    60421 ggatggactg gaaaccctaa tcatgtcaaa tataaaattg aatggttaaa tggtctatct
    60481 tatgtcggag atttctggaa tagaagtcat ctcattgcag atagtctcgg tggagatgca
    60541 ctcagagtca atgccgttac aggaacacgt acccaaaatg taggaggtcg tgaccaaaaa
    60601 ggcggcatgc gctataccga acaaagagct caagaatggt tagaagcaaa tcgtgatggc
    60661 tatctttatt atgaagccgc tccaatctac aacgcagacg agttgattcc aagagctgtc
    60721 gtggtatcaa tgcaatcttc tgataatacc atcaacgaga aagtattagt ttacaacaca
    60781 gctaatggct acaccattaa ctaccataac ggtacaccta ctcaaaaata ataccaaaag
    60841 gctagacctc tgctcactag gcctagcttt ttacatcaaa aaaagcaatg actatagaaa
    60901 gtaaaaatac tagaaaaagc aatgattgcc gtcattgctt tttatgattt gtcaaaaagt
    60961 aaaaagcaaa ttgaaaaaat cgccctggag ctgttgagat aaactacacc atttattaag
    61021 ataccaaatc aaaaagctaa caccataaga gcgttagctt tttaaagagc ctcaggacag
    61081 tttatgttta atggcttcta ggtaggtttg aaacatatga tggatcgttt tgcaattgag
    61141 taattggaag atctctaaga tctgcacaca cttttgctca gccccctctt taccatttat
    61201 gaggccaata atgccatcaa aaaaagacac acaacaccgt tcatataagc aatcatcatt
    61261 tagctgataa tcttcaattt ttgccaaaat ctcagaagct ttatcatact cttgtcgctg
    61321 aataaacaaa atcaacatat tgacaaacat ccgaatcgat tcattcccat aataccttag
    61381 ggtattgtat ttatcgaggt tcaaaataac ttttgaaaac accatctcaa taaaacaaga
    61441 ctcaaaaata aacatacaat tattaaaaag cacagtctca tagtgactcc aagtttcaat
    61501 attaataaga tagtttgaca aataagtccg ctctgtcaga cagtcttcct cagtaagagt
    61561 tgctaataac accttgacca aggcaaaaag gttcttttcc tttgttgact tactatcttt
    61621 ataagaatca aggatatgat ttaaaccttc tatgtttcgg cattcaaaat aatgttttgc
    61681 cgtatccata tcaataaatt ctttatattg tttaaagtta ttactaataa agagaaactc
    61741 atcaacattg acattcaagt tgtctaagat ataaagtaac tttttcgaag aaatgtcaag
    61801 attattttta ataaaacgat aataggtttg cctagtgaga taatcaccac aaacttgttt
    61861 aatcgaaatg ttttttgaat gcctaatgaa ttcaacggtt tcaccaattt ccatatgtca
    61921 agccttccta gttgatgtca aaaatacgtt acgcatgtac ctaatacgta acaagttgaa
    61981 tgtttcggat gatagtcgct tatgataggt gcataaggtc aatagccaga tgcgatagtt
    62041 ttatcaactg tcatatgtta aacctttcta atctaataga tgttaaaaat acgttacgtg
    62101 tgtacctaat acgtaacaaa ataatgggtt agcaaaataa gcagctatga tatagccata
    62161 aggttaaaag gaggcgccta ctatgtggtt attgttacta tttttgtagt tccttttgca
    62221 aaatggtagt agataaaaat gagaagtcaa taaaagcatt gacaaacaaa agaatcattt
    62281 tgtttatatt atcagtattt ttaagtttct tgtcaactga aatgagcatc tactagccac
    62341 aatagtaact aaaaggctaa ttgtcagtaa aaatgagata aatcagccag gaaaaagcct
    62401 acgagtcaac taatggacaa ccccaaaaaa gacaaaggac tttcacttgg tgttgtggac
    62461 cctatcacga tccttatcac agaatcagtt attaacaaaa tgagaacggt cccatatcat
    62521 ctaaaaaaac tgtcaagcac aacctattgt ttgctagctg atcttgccta tcagtctgcc
    62581 tagtagatac ggcagaagat aagagatcaa ccaaaagatg ataagtgcaa cgactcatag
    62641 cgtcttttga ccaaaaccaa ttagactcac atgagatgag aataattggg ttgggttgtc
    62701 agtgtcaact aaccgtgtta ttgtctatta ccattcatgg tatcagcgac atcgtatgat
    62761 aaccatgcga ttcagctaag taaggaggtg tgtccaatgt actgttaaaa gcaaatgcag
    62821 tagattaact tattttgaaa gaggtataaa aaaaatgaat aaaaagaaat taggtatcag
    62881 attattaagt cttttagcat taggtggatt tgttcttgct aacccagtat ttgccgatca
    62941 aaactttgct cgtaacgaaa aagaagcaaa agatagcgct atcacattta tccaaaaatc
    63001 agcagctatc aaagcaggtg cacgaagcgc agaagatatt aagcttgaca aagttaactt
    63061 aggtggagaa ctttctggct ctaatatgta tgtttacaat atttctactg gaggatttgt
    63121 tatcgtttca ggagataaac gttctccaga aattctagga tactctacca gcggatcatt
    63181 tgacgctaac ggtaaagaaa acattgcttc cttcatggaa agttatgtcg aacaaatcaa
    63241 agaaaataaa aaattagaca ctacttatgc tggtaccgct gagattaaac aaccagttgt
    63301 taaatctctc cttgattcaa aaggcattca ttacaatcaa ggtaaccctt acaacctatt
    63361 gacacctgtt attgaaaaag taaaaccagg tgaacaatct tttgtaggtc aacatgcagc
    63421 tacaggatgt gttgctactg caactgctca aattatgaaa tatcataatt accctaacaa
    63481 agggttgaaa gactacactt acacactaag ctcaaataac ccatatttca accatcctaa
    63541 gaacttgttt gcagctatct ctactagaca atacaactgg aacaacatcc tacctactta
    63601 tagcggaaga gaatctaacg ttcaaaaaat ggcgatttca gaattgatgg ctgatgttgg
    63661 tatttcagta gacatggatt atggtccatc tagtggttct gcaggtagct ctcgtgttca
    63721 aagagccttg aaagaaaact ttggctacaa ccaatctgtt caccaaatca accgtagtga
    63781 ctttagcaaa caagattggg aatcacaaat tgacaaagaa ttatctcaaa accaaccagt
    63841 atactaccaa ggtgtcggta aagtaggcgg acatgccttt gttatcgatg gtgctgacgg
    63901 acgtaacttc taccatgtta actggggttg gggtggagtc tctgacggct tcttccgtct
    63961 tgacgcacta aacccttcag ctcttggtac tggtggcggc gcaggcggct tcaacggtta
    64021 ccaaagtgct gttgtaggca tcaaacctta gtatggaaat gcatttcgtt agaacagaac
    64081 ctgaggcacg ccgcatagct gaaacctttt gtgccgaaaa cacacaaaca aaaaccccta
    64141 tgcgcgtgca gcagttaagc tatccatcag acacagatca ctcaggtggt gagctataca
    64201 tctatgcttt gtctcctgct ggatttatca tcgtatcagg agacaccaga gcgcacacca
    64261 ttttaggcta ttcttttgat aataacctag acctcaacca tgataatgtc aggagtatga
    64321 tagaagctta ccaaacacaa atcaactctt tggattgatc ggtcaaagta gcttttgccc
    64381 ccctcaccac tcgtttaaga cgaccgtgtt tctaaaatac cactaccttt taagtttcac
    64441 gccggcctaa gcgagtgttt gtctcatgga gcagactgtt tccatcgaaa agtcacccat
    64501 tgataaagga ggtcatcaac gcctactaat gatgacttcc ctaagatgcc aaggtaaagg
    64561 actgagttct gtagagatgg gactcagtcc ttttacgatt tttgggagtt attctagcca
    64621 taccgtggca agaaaacaac acttaaaaat agccaacgtc agtttctgtt catacatgac
    64681 tacagccaaa caccacaacc taacctatcc taaacacagt ggctttctaa cgaacagtca
    64741 caacgagcag caacaccaaa aagcaagatt aagctacgtt taagactaat gatataagat
    64801 aacagtaatt attaatgaga aaagaggctc aggacatgaa acaaatgaat aaactcatta
    64861 caggagtggt aacgctggcg acagtcgtga ccttatcagc ttgtcaatca tcacacaaca
    64921 acaccaaact cgtctcgatg aaaggagaca ccatcactgt cagtgacttc tacaatgaga
    64981 ccaaaaacac agaactcgca caaaaagcca tgttaagctt ggtgattagc cgtgtatttg
    65041 agacacaata tgccaacaaa gtctctgaca aagaggttga aaaagcctat aaacaaaccg
    65101 cagaccaata cggtacatcc tttaagacag tcctagcaca atcaggctta acgccagaaa
    65161 cctataaaaa acaaattcgc ctcacaaaat tagtcgaata tgccgtcaaa gaacaagcca
    65221 aaaacgaaac catctcaaaa aaagactacc gtcaggccta tgacgcttat accccaacca
    65281 tgaccgcaga aatcatgcag tttgaaaaag aagaggatgc caaagcagcc cttgaagccg
    65341 tcaaagctga aggggcagac tttgcagcta ttgccaaaga aaaaaccatt gcagccgata
    65401 aaaaaacaac ctacacgttt gactcaggtg aaacaaccct accagcagaa gtagttagag
    65461 ctgcatcagg cctcaaagaa gggaacagat cagaaatcat cacagcgctt gatccagcca
    65521 cctcaaaacg cacctaccat atcatcaaag tcaccaaaaa agcaactaaa aaagcagact
    65581 ggaaagcgta ccaaaaacgc ttgaaagaca tcatcgtgac tggcaaatta aaagaccctg
    65641 acttccaaaa caaagtcatc gctaaagctc ttgataaagc aaatgtcaaa atcaaagaca
    65701 aagcatttgc caatatctta gcccagtttg caaaacctaa ccaaaaacaa cctgcccaaa
    65761 aatagagggt tgccagttta tttccctaag gttgcttaaa aagacagccc taaccatcac
    65821 tccccttttc aaaacaccga taatggaaac gtgtcctata ctgatggtct agggcaatgt
    65881 cataaagcta aagactctat ccaaataagg atagagtctt taatatttaa aaaccagtca
    65941 gcaaaaaaaa ttcacgctaa gcctagctag aatgaggctt agcgtgaatg gtatcagtga
    66001 tagtccgtta acatagctga ttgggagtca gaaactctaa ctacccaatc tgactatacc
    66061 cccagtacca gaggtgtctc atcgcagaag tagcaatcag ataatcgtct gataagctct
    66121 tgtcctcaag caactgatga atatcgtcgc acgggccatg agctgccggt ggctgatagc
    66181 tagtaggctc atcagccaaa acttttttgg agtaacgctt tggtctcaga cggcgccaga
    66241 gggttgagag aggggtgaga aaaaaacgat gggtcatctt ggcctccttg tctaatgata
    66301 gtcaccttaa cagacaaaag tgtcaataat gtcattttaa ggagaaaaag attaaaaatc
    66361 aaggtcacaa actttccttt gttaaaaaaa cgcgacccta acaaagaatc taaatacctc
    66421 tcgcacttga gcttctcctt gcgcagctat cttgtgcact cagttgcctc caagataatg
    66481 ataacgatac catcattatc ctataggcta tcgcgacaac cccttttttc gcctcaacga
    66541 gtctgacaca gctgtcatga cagaaaaaag acctctgcct atctaagaga ccattaattt
    66601 ttctctcact aaaaaactga ttttagaaac gaagaagcat ggtgtataat aaggttcaga
    66661 acaattatga cataataatg aaagtaaggt taacgaaaca ttgacaaatt gtaagtataa
    66721 acttagaaag ttatctgtag ggctcgtctc cgtcggaacg atgctgatag ccccgacagt
    66781 tttaggacag gaggttagtg ctagtagtag tacggagagc agtaccacta cagctaatac
    66841 tggtaccggt acggcaagtg ggatgactgc cactactcct agtgctacga cagatactgg
    66901 tgaagcagct gggagcggag ctaggagtga agctaatggt gcatcgtccg tagtatctag
    66961 cgaagaaagt cagagttcag gcactactcc agcctcaccc caagcacaga cagctccagc
    67021 agcaacgtca acatcatcgg cttcttctag taatgagaaa actcccaaga cagcaactac
    67081 aactacatca tcgactccag tagcaagtac cagtaataat agcaacaaag taactagtac
    67141 tgaagctgaa acacagacga tggacgtgga acggtataca gttgataagg aaaattcaaa
    67201 gctaaatatt aaagacggta agactccaaa aactaggagt agtgttaata aagacacaaa
    67261 acttattaga aaccgcgatg acaaacagcg tgatatcgtt gatgttactc ggacagttaa
    67321 aactaacgaa gatggcacta ttgatgttac cgtaacggtt aaaccgaagc aaattgacga
    67381 aggcgccgat gttatggccc ttctagatgt ctctcaaaag atgacaaaag agaattttga
    67441 taaggctaaa gaacaaataa aaaaaatggt tacgacttta acaggcgagc caactgatgg
    67501 taaggaaaat cataataggc gtaattctgt acgtctaatg actttttacc gtaaggttaa
    67561 tgagcctatc gaattgactg ctgaaaatgt agacaaaaca ttagatgaag tttggaaaaa
    67621 agctaaagaa gattgggact ggggcgttga cttacaggga gctatccata aagctcgaga
    67681 gatttttaat aaggaaaaag aaaaaaaatc gggtaaacgc cagcatatcg tcttgttctc
    67741 tcaaggcgaa tcaaccttta gttatgatat taaaaataaa agtgatatta caaaaacaaa
    67801 aataactgaa aaagttacga cctctagccc actgtttcct tggctcccta tctttaatca
    67861 cactaaccga aaggcagaga ttattggtga tttagaaaaa gtactagaca tggccgaaaa
    67921 agtgggaata agtttaccta gtagtctgaa gtcagcagtg aaagcccttg gcttaactaa
    67981 tagtgcaata ggttctattt tagggaaagg tttgacagag taccttggtt tgacagaata
    68041 tagttcagat aacttagatg gaggagggtt tgattatagt aaacgtgtag gggaaggtta
    68101 ctactaccac agtttttcag ataggaaata tgaaaataca atgccccttg aagaagctat
    68161 caggacggcc ttagcatcta atttttccaa actcacagat aattggtttt tcgatatctt
    68221 aaatagtttt gtcaataaag atacagttga gaaagctaaa ttagacgtaa ttatgaaggt
    68281 acttaatagt attttttaca aacgtgaata tcgctattac aaccataacc tgtcagcaat
    68341 agccgaagct aaaatggctc aacaagaggg cattaccttc tattccgttg atgttactga
    68401 tttaaactca gcttctaaaa gagtgaggcg acaagcagca gtgcgtaagg gcactaagga
    68461 agaagaaaac aaaaaaaatg aagaagagcg taatacaaag tttgacacct atctgaaaaa
    68521 gatgtctgaa ggtaataatt tcttaagtaa tgttgaagag agagataagt tcaaagatac
    68581 tttaacagag ctgacaatta aagacgaatt tacggataaa gttacggtcg agaaggattc
    68641 ctggagtaaa tctataactg atggacttaa aaattcaaat aacaataatg ttaaacatca
    68701 acaggcaaac acatcgacat ggagtttctt cagttcatcc aaagaaagcc tcacctggat
    68761 tatttccaaa gagcagctca aagaagcctt tgagaaaaat ggttctctca cttttaaata
    68821 caaattacgg gtcaataaag acaaactatt agataaagat aagaagagaa caaaacgtga
    68881 tacacctacg gaaaataaga cttctgtaac ggcagatatc atttcaaata ctgttgacta
    68941 caaaattaat aatcaagaag ttaagggtaa caaacttgat gctgtcaagt tgacttatac
    69001 taaagagacc gttcctgttc cagatgtgga aggagaagtt gtaccaatac cagaaaaacc
    69061 actggtagaa ccaatgacgc ctctataccc tgcaattcct aattacccta cccctcaact
    69121 tccaaaagat gaagatctgg agattagtgg aggtcatgga ccgattgtcg atatcgtcga
    69181 agatactggt acaggtgttg agggcggcgc tcaaaatggt gtggtttcaa ctcaggaaaa
    69241 taaagatcca atcgttgaca tcaccgaaga tacccaacca ggtatgtcag gctcaaatga
    69301 cgcgacagtt gtcgaggaag acacagcacc taaacgtcca gatgtccttg ttggtggtca
    69361 aagtgatcca atcgatatca ccgaagatac ccaaccaagt gtgtcaggct caaatgacgc
    69421 gacagttgtc gaggaagaca cagtacctaa acgtccagat atccttgttg gcggtcaaag
    69481 tgatccaatc gatatcaccg aagataccca accaggcatg tcaggttcta atgacgctac
    69541 tgttatcgaa gaagatacga aaccaaaacg cttcttccac tttgataacg agccacaagc
    69601 accagaaaaa cctaaagagc aaccatctct cagcttacca caagctccag tctataaggc
    69661 agctcatcac ttgcctgcat ctggagacaa acgtgaagca tcctttacaa ttgttgctct
    69721 aacaattatt ggagctgcag gtttgctcag caaaaaacgt cgcgacaccg aagaaaacta
    69781 accctcgtta gtctagcgac cctaagcctt tggctttaag aatttctcat tatccccctc
    69841 aaacaccggt acctcactga gatggcgctt taaaaaggcg ctaatctcaa atcaggtatc
    69901 ccctacggtt gagggggatt aatgggtatc acaacaagaa aaaaggtata atgatgaaat
    69961 atgactaaaa aatttattaa atcagcagtg attctaggct tagcaagcat aaccatcagc
    70021 atgacgcaac ccgttgaagc catttcaaat agcttagaat atcaagcaca atacgaacgt
    70081 gtcaaagaag aactagacgc attaaaagaa aagggcccaa aattaggaga acgttccctt
    70141 tattttagat tggtgaataa atttctaagt tggtttggcc aagtctggga cccactcgca
    70201 atcaaagaaa ccgattacct gttggacctc atcggacaag tcaattactt ggtgagtaac
    70261 accgagtttc aagcccctga gtacaaagat gctaaaaaca aactagactt agctgtagca
    70321 ggctatgacc tctacctagc tagagagaaa aaacgtggtg atgctcctga ttaccaagtg
    70381 acccagctaa cgctagaaca gctcaaaacc atcaaaaaga agatacgaga ggccctcgaa
    70441 acatttgcac aagaggtcaa aacaatccag caaaaaaacc cagacctatt tccccatgct
    70501 tatgttttaa acgctaagag caaagatatc acagataaag cgcaaccctt tttagtaaat
    70561 agttactacg atgtctttta taagcagttt ttattcacta atacaaatga cgggtacaac
    70621 tataataaag aagcagacaa tctgacctcg tatcaaaatc tgattaaaga gctattaggc
    70681 ttaaaagctg agatcgatca gttaacaaaa ggcaagactg gcgatgaccg tttagagatg
    70741 ttaatgtggc aatcaaagtt caccttatct gattcacgga aaaaatcaca ccttagcgcc
    70801 tactatacac aaggcgagta tgtcgataag gtggtcctat ctgaagaagc tcccaacttg
    70861 agaacagcaa aagaaaaggt caagacctta aaagggatct tgcatgatta ctatgttgaa
    70921 actaataagc cagaaaaagc taaaaagtat catgtcgagg acgagacctt gccgtctcaa
    70981 cctgaggctc ctgctacacc tgaagctcct gctcctgctc ctgttcctgt tcctggtcaa
    71041 aaaccagcag aagatgagag atcgccacaa gctacggagc cagctaaccc gtcaaaagag
    71101 gattcctcaa cggacgcatc tcagggatct cacgatagtg aaaacccagc gacagacagt
    71161 ccttctcaac ctcaggcacc agaccaaggc aatcaccaaa gtcaagtgcc taacgccaag
    71221 cctcaaacgg acaagacgga cactcctaac gccccagcca tcccacagga caccccaaaa
    71281 gcgcctgagg ctggtggtca gagtggcccc gctgggaaca ctgaggagaa agctcctgac
    71341 tctggaccta aagagtctga tcaaagctca tctaaagagt ccccatccgt tggcgaagac
    71401 acgaccccta atcagccaga tgtccttgta ggtggtcaaa gtgagccaat tgatatcacc
    71461 gaagacacaa tcactgacgc tcctccgaca gtatctgggc acaacgcatc aacacagccc
    71521 caatctgtgg tcgaagacac agcacctcag cgcccagatg tccttgtagg tggtcaaagc
    71581 gagccaatcg atatcaccca agatacccaa ccgggcatgt caggctctaa tgacgctacc
    71641 gttatcaatg aagatacaaa accaaaacgc gtcttccact ttgacaacaa agagccacaa
    71701 gcatcagaaa aagcggcaga gcaaaaactt gctcctcatg atagccacac aacccctcaa
    71761 gcttcagatg atacagcagc ccctcacttg cctgcatctg gagataaacc tgaaaaagtg
    71821 tttgcacttg ctgccttgac aatggtcgca gctgcagggc tactcagcaa aaaacgtcgc
    71881 aacaccgaca aaaactagtc gctgttaggc acaacgcatc actaaggcca gtgcatttgc
    71941 actggcctta gttgggtttt tatcaaatag agggtttttc atgacaattg ttagcacttc
    72001 ggtgtaaaat aaaagtaata accagcaaag gagcaaccta tgacaaagca agatcagtta
    72061 atcgttgaaa aaatggaaca aacctatgag gcctttagcc caaagctagc aaatctcata
    72121 gaagccctag atgcctttaa agagcactat gaggaatatg ccacacttag aaatttttac
    72181 agtagtgacg aatggtttcg gcttgcaaac caaccttggg atgatatccc gtgcggtgtc
    72241 ctatctgaag acctcctctt tgatatgatc ggtgaccata accaactact agctgatgtc
    72301 ttagacttgg cccctatcat gtacaagcat atgtaaccat tgttaacgtc acagatacta
    72361 cgatgacagg acaaaaaaag cttgaaaatc tcaaaaaaaa acagataaaa aacagataat
    72421 cgttagaaaa acagtagatg cgtctgttaa actaaaggta tcaaaacaaa aggagcattt
    72481 catcatgaaa ttcaaaaaag tattagtcat tcctgcatta gcattagcag caacatgctt
    72541 tttaacagct tgtggcacaa aaaaagattc taaaaaagaa gaagttaaag aaatcaaaat
    72601 gtctgacatc aaagacgacg cagttagcaa aaaaacaaaa gtggttgatg gcgaagaagt
    72661 tacagaatac accacaaaag atggaaacgt gattcagatt cctgcaggaa acgaagaagg
    72721 catggaatca aaagatgctg gtggatcagg cgctcctgct aaaaactaag ggttacaaca
    72781 aagagcataa ggtgactcgc aaacgttacg catgacatga ggcagtctgc ctaagatggt
    72841 aactcactta tcataatgcc taaatagcca aacacactaa ctcgcaaatg tacaaaatca
    72901 tatacccgtg cttatgtgag cgaacggtta tatgattttt gttttccaaa aacaagaaaa
    72961 gaacgctaaa atagccagtt tgtcgtgttt caagctacca aaaaggaagt gctaagaagt
    73021 gctaaaatga aaaaaagatc cattatacct gtcatcttac tgttggtcat cggattaatc
    73081 gttggtgtta agcgtcatca tgactagtag gtcttgctag tgaccgttca aaagggataa
    73141 cgttggtatg gtataaccat aaaaatcgta taaaaagaaa aaatagctat tgaggagaaa
    73201 gtatgtttca gttaagaaaa aaaatgacgc gcaaacaatt agccttgttg agtgctggag
    73261 tgttgacctg tgtggttggt ggtagctact tgataatgaa ccatcaacaa caagaaattg
    73321 tctctagtgt caacaaagta aaagccttaa ccataaaaga agccatggaa caaggaaaag
    73381 atatcagctt gaccttagct ggcgaagtaa cagctaacaa cagcagcaaa gtcaaaatcg
    73441 actcaagtaa aggagaagtc aaagatgtct ttgtcaaaaa aggcgatgtt gtcaaagtag
    73501 ggcaaccctt gtttagttat gaaacgtcac aacggttaac ggctcaaagt tcagaatttg
    73561 atgttcaaac caaagccaat caactccaag ttgctaaaac caatgcagca ttgaagtggg
    73621 aaacctacaa tcgcaaggtc aatgaaatca ataccctaaa atctcgctac aacactgcac
    73681 cagatgagag cttactagag caaattcgca gcgcagaaga cagtgtatcc caagcactaa
    73741 gcgatgccaa aacagcagat agcgatgtca aaaccgctca aatcgaactc gataaagcta
    73801 atgctactgc cacaacggaa aaaggtaaac tagagtatga cactgttaag tcagacactg
    73861 caggaaccat tgttagccta aatactgatt tgccaaatca atcaaaatcc aaaaaagaaa
    73921 atgaaacttt tatggaaatt atcgacaaat caaaaatgtt agtcaaaggt aacatcagtg
    73981 aatttgaccg tgacaagtta aaaatcggtc aaaaagtcga agtgattgac cgcaaagaca
    74041 actctaaaaa atggactgga aaagtaaccc aagttggcaa cctcaaagca gaggaaaaag
    74101 gccaaggtca aggccaaggt ggcaatgacc aacaagacaa tccaaaccaa gcaaaattcc
    74161 cttatgttat cgaacttgac caatcagaca agcagccact cattggttca cacacctatg
    74221 ttaatgtgct caacaatgtt ccagaagctg gcaagatcgt attgaaagaa acctttacaa
    74281 tggcagaaaa tggaaaaacc tatgtgtgga aagttgataa aaacaaggtc aaaaaacaag
    74341 aaatcaagac taagcccttc tcaaaaggtt atgttgaggt gacaagtggc ttgactatgc
    74401 aagataagat tgctcagccg cttcctggca tgaaagacgg tatggaggta ggaagtattg
    74461 ttaaacctta aagatattcg aaaaagctat catcttggga ctgaagaatt tgcgatttta
    74521 aaaggaatcg atttagaagt taacgagggt gactttttag ccatcatggg accatcaggt
    74581 tcgggaaagt caacattgat gaatatcatt gggtgtttag ataagcctgg ctctggctca
    74641 tatgccattg aaggcagaga cgtgtcatcc ttatctgata atgaacttgc tgatttgcgt
    74701 aatcaaaaaa tcggctttgt ttttcaaaac tttaacctga tgcccaagct aacagcttgt
    74761 caaaatgtcg aattgccctt gacttatatg aatgttccta aaaaagagcg tcgcaaacga
    74821 gcgctagaga tgttaaagct agtaggacta gaagaacgca gtgaatttaa accgatggag
    74881 ctatctggtg ggcaaaaaca gcgtgtagcg attgcaagag ctttagtcac taacccgagt
    74941 tttatccttg gtgatgagcc aacaggtgca ctagacacaa aaaccagcgt ccaaatcatg
    75001 gacctattta aacaattcaa tgataacggc aaaacgatta ttatcatcac acacgagcct
    75061 gaagtagctg ccttatgcaa aaagacggtg atcctaagag atggtaatat agaacattcc
    75121 gatatagagt aaagggggaa aataatggaa gatattattg cagctttaag ctctatatta
    75181 tcacacaaga tgcggtcaat tttgacaatg ctagggatta ttatcggtat tggtgccatt
    75241 atcgcaatct tttcaatcat cgaaggtaat accgaaaata ccaaaagaca gttgattggt
    75301 ggcagcaata acactattaa cattgtgttt aacaaaaaaa gcagtattga ccctaaattt
    75361 ccagacaagt caaatgctaa aaaaccagac tatcttcctt ttatggctga agaagagctt
    75421 agcaaaatac agcaagtcaa aggtgtcaaa aacgccctga tctcatatgg tattgatgac
    75481 aaagtctacc atctaggcca aaaatcatct gcaaagattt ctgccatcac aaaaaacgta
    75541 gctgaagtga ggcgcatgac atttattaaa ggcagtgact tttcagacaa agactttatt
    75601 gaccaaaaac aggtgattta ccttgaaaaa agcttgtatg agtcactatt tccaaaagac
    75661 gatggccttg gaaaatttgt cgaagtgatg ggaaatccct tcagggtcat tggggtgttt
    75721 gaatcaaaag aacaaagtgg cctcacaagt gggactgaga aaattgctta tatcccgcta
    75781 catcagtggt ataatattaa tggtgtagtg gatgcgacac ctgagatcac gatccagacc
    75841 tatcgcgcag atgacctcaa acctgtagca aagcgtgtca gtgatatgct caatcaaacg
    75901 atccctaaat cagattatat gtttggtgtc atgaacctta aagaatttga gcgccaacta
    75961 gacaacctaa acaaatctaa ttttgtgtta ttagctggta ttgctagcat ctctcttatt
    76021 gtaggtggta ttggtgtgat gaacattatg ctagtatcag ttaccgaaag aacgagagaa
    76081 attgggatca aaaaagcgct tggcgctaga cgtaagctta ttttaaaaca atttttaatt
    76141 gaagcggtca ttctaacctt gctaggtggt gtgatagggg ttatttcagg gatggtatca
    76201 ggattgatca tcacaagatc actcgagtat ccctatatcc tatccttgtt ctcagttgtc
    76261 ttgagtttag cgttttgttg tattataggg attgtctttg gactattacc agccattaaa
    76321 gcgtctaagc tagacccaat tgaagcattg cgatttgaat aatcaaccaa acaagcataa
    76381 aagtcaaaaa ggatgaccct ttgatgtcat ccttagctgc ttgatacact aaacgccaat
    76441 ctagaaaggg aagttatgtt taaaatatta gtagttgaag atgatgatac catcagtcaa
    76501 gtgatttgtg agtttttaaa agcgaataac tatgatccag attgtgtatt tgatggccaa
    76561 gctgccttag acaagtggca gacaacgtct tatgatctga ttattttaga catcatgctc
    76621 ccttccttaa gtggcttaga agtgctaaaa acaattcgaa agacatcaga tgtccctatt
    76681 attatgctaa cggcattaga tgacgaatac acacagcttg taagcttcaa ccatttgatt
    76741 agtgattatg tgaccaaacc attttcacca ctcattttaa tcaaacgtat cgaaaatgta
    76801 ttacgcgtct ctactccaga tgaaaagcgc caaatcggag acttacttgt tgatgagact
    76861 gaacatagcg tctattggca aggaacattg gtcaaactaa ccaaaaaaga atatgacatt
    76921 attgattatt tagcaaaacg tcaccaaaaa attgtcacca gagaccaatt gatggatgac
    76981 atatggggct attctgaact ggatacgcgt gtccttgaca accacattaa gaaccttcgt
    77041 aaaaaaatga cgggtattcc cctcaagaca attacaggca tgggctattt attgggagag
    77101 cgtgagtgag actaatcaaa aaaacctttt tggtcatcaa tggcttaatt attgtagtgg
    77161 ttacctctat tttgttagtg ctttattttg caatgccaat ttattacacc aaagtcaaag
    77221 acaaagaagt taagcgtgaa tttgaccaaa caagcaagca aatcaaaggc aaaacggtaa
    77281 ccgagattag agatatccta acaaaaaaaa tcaacaaaga taatatttgg tacagcttag
    77341 tagacagtga taaccaactg ctctatcctt ccttgcagct gttagacggc gtcagtgaaa
    77401 gcaaagacag tcaaaatgtt aatatcgtca ccacctttga caactcttat tctaatgtta
    77461 aggtgatgag tcaaaaagta accctacgcg atggaaaaaa aatgacattg cttgggcaat
    77521 cttcacttca acccgtaaca gatgctagca aagttctcct tgacctctac ccgtcactat
    77581 tgatgttttc agtgaccgta gggagtattg tcgcctactt atatagtagg acttctagcc
    77641 ggcgtatcct tagcatgtca caaaccgcca aaaaaatggt caacctagaa ccaaacttaa
    77701 cgtgtaccat tcacggcaaa gatgaaatag caatgttggc tagtgatatt aaccgtttgt
    77761 atgcgagtct ttcaacgagt atcaaatcct tgcaaaaaga gtatgaaaaa gcttctgatt
    77821 cagaaagaga gaaatctgag tttttacgca tgacatcgca cgagctaaag acaccaatca
    77881 ccagtgttat cggaatgatt gatggaatgc tctataatgt tggtgacttt gcagatcgtg
    77941 acaaatattt gcgaaaatgc cgagacgtcc ttgaagggca agcccaactg gttcaatcca
    78001 ttttatcttt atctaagatt gaaaccctag cttctcaaaa tcaagaactg ttttcactaa
    78061 aaagcagcct agaagaagaa atggaagtct ttctcgtctt atcagaacta aaacatctca
    78121 aagtgactat caatctcgaa gaacaattcg tcaaagccaa taaagtatac ctactaaagg
    78181 cgattaaaaa tattattgac aatgcctttc actataccaa atcaggcggc caagtgatga
    78241 ttcaactaaa agacaaccaa ctagtgatta aaaatgaagc agagacatta ttgacacaac
    78301 agcagatgaa acagttattc caaccgtttt atcgaccaga ttatagtcgt aacaggaaag
    78361 acggtggtac aggattgggg ctgtttatca cccaccagat tcttgatcag catcatctgg
    78421 cttatcgctt tgttgttctt gatcaaagat ggatggtatt tacgattgat tttccatccc
    78481 atcatgacga ttgatccaca ctagtaggac gacaaaagtc atcaatcact aaccaagcct
    78541 tgacctttac ccatcattgt ttaggcaagt ataaaaaaga ggagagaaat catatatgaa
    78601 gaaaaggaaa ttgttagcag taacactatt aagtaccata ctcttaaaca gtgcagtgcc
    78661 attagttgtt gctgacacct ccttgcgtaa taacacatca tccactgatc agcctactac
    78721 agcagatact gatacggatg acgagagtga aacagcaaaa aaagacaaaa aaagcaagga
    78781 aacagcgtcg cagcacgaca cccaaaaaga ccataagtca tcacacactc acctaacccc
    78841 cccttcaaat gatactaagc agaccgatca ggcatcatct gaagctactg acaaaccaaa
    78901 taaagacaaa aacgacacca agcaaccaga cagcagtgat caatccaccc catctcccaa
    78961 agaccagtcg tctcaaaaag agtcacaaaa caaagatggc cgacctaccc catcacctga
    79021 tcagcaaaaa gatcagacac ctgataaaac accagaaaaa tcagctgata aaacccctga
    79081 aaaaggacca gaaaaagcaa ctgataaaac accagaacca aatcgtgacg ctccaaaacc
    79141 catccaacct cctttagcag cagctcctgt ctttgcaccg tggagagaaa gtgacaaaga
    79201 cctgagcaag ctaaaaccaa gcagtcgctc atcagcggct tacgtgagac actggacagg
    79261 tgactctgcc tacactcaca acctgttgtc acgccgttat gggattactg ctgaacagct
    79321 agatggtttt ttgaacagtc taggtattca ctatgataaa gaacgcttaa acgggaagcg
    79381 tttattagaa tgggaaaaac taacaggact agacgttcga gctatcgtag ctattgcaat
    79441 ggcagaaagc tcactaggta ctcagggagt tgctaaagaa aaaggagcca atatgtttgg
    79501 ttatggcgcc tttgacttca acccaaacaa tgccaaaaaa tacagcgatg aggttgctat
    79561 tcgtcatatg gtagaagaca ccatcattgc caacaaaaac caaacctttg aaagacaaga
    79621 cctcaaagca aaaaaatggt cactaggcca attggacacc ttgattgatg gtggggttta
    79681 ctttacagat acaagtggca gtgggcaaag acgagcagat atcatgatca aactcgacca
    79741 atggatagat gatcatggaa gcacacctga gattccagaa catctcaaga taacttccgg
    79801 gacacaattt agcgaagtgc ctgtaggtta taaaagaagt cagccacaaa acgttttgac
    79861 ctacaagtca gagacctaca gctttggcca atgcacttgg tacgcctata atcgtgtcaa
    79921 agagttaggt tatcaagtcg acaggtacat gggtaacggt ggcgactggc agcgcaaacc
    79981 aggctttgtg accacccata aacctaaagt gggctatgtc gtctcatttg caccaggcca
    80041 agcaggagca gatgcaacct atggtcacgt tgctgttgta gagcaaatca aagaagatgg
    80101 ttctatctta atttcagagt caaatgttat gggactaggc accatttcct atcggacgtt
    80161 cacagctgag caggctcgtt tgttgaccta tgtcgtaggg gacaaactcc caagaccata
    80221 accccacaac caagaagcta cctgcctgtt gaccaatctc aaaaagagac agtgtcaact
    80281 tagaatgagg ttcatcagat gataaagaaa gtgaccaccc cgagtcaaaa aataaaaaaa
    80341 cgtgttcgta atggctatct cctaaaacta ggtacagcct gtttactctt aagtatactc
    80401 agttatggca ttggtctttt gggccagcct agcatggaaa atacgtttat gggaatagcg
    80461 agtgttgcca tgttaggaag tgtctgtttc tttattatct ttgctctaaa ccgtatcttt
    80521 gacgcactag aggacaattt gcgagattag agtaataggt caaataatct tccaataatt
    80581 tagagcacaa gctaagttat tggaagtttt tttgtctttt gagagccatg atgccatcaa
    80641 aaagtcaaac actccttatg atgtgagagt cacatgacta aaattaaaag caaaatagac
    80701 cccaaagccc tgttttctca aaacagccaa cgaaaaaaga caagttaacc agttcataaa
    80761 aaacatcgta gaaacgtttt caagagctaa tgttggagta aattgactga agtatgatag
    80821 aatttttaat gaatgtgaca ataatgtcac agataaaaac cgaaaacagc aaaaataatt
    80881 aaattgaata taaaaaagtt aattaagtga gtgataatca agaaatgaag ttcaaaaaat
    80941 ataaataaaa taaaacacaa gaactgctaa aagcagtgaa tgacataaat gtcgctaaaa
    81001 aaatagatag aagagtctaa aatttcagtt ttaggtcacg ttttatgaat ggaaatcaga
    81061 atgatgacct taagttgtga tttgcgcgtc attgaccgct ttcttcgttt taatgaaaga
    81121 atttgaaaga tattttatgg tatacttttc cttaatatgg ttcatacgga cttgataaat
    81181 ctatttacaa aaataataaa aaattaagat aaggacatga agttaatgca tgtaagtaaa
    81241 ttgtttacta gccaacaatg gagagaattg aaactgatct catatttaac agaaaactcc
    81301 aatgctatcg gagttaaaga taaggagctt agcaaggctc tcaatatttc catgttaaca
    81361 ttacagtcat gcctcactaa tatgcagttt atgaaagaag taggtggaat tacctacaaa
    81421 gacggctata ttaacatttg gtatcaccag tgttgtgggc tacaagaggt ctatcaaaaa
    81481 gctcttcgag aatcgccttc tttgaaatta ctggagcttt tgtttttcag agactttagt
    81541 tccttagaag agttagctga agagctgttt gtcagcttat ctaccctcaa acgcctcatc
    81601 aaaaaaacca acacttactt gtctcatacc tttgctatta gtatcgtgac aagtccagtg
    81661 caggtgtcag gagatgagag gcaaattcgc ctgttttacc tcaagtattt ttcagaagcc
    81721 tataaaatct cagaatggcc atttggggac attttaaacc tgaaaaactg tgaacgccta
    81781 ttgagcttat tgatcaaaga agtagatgtc aaagttcatt ttacgctatt tcagcactta
    81841 aaaatcttaa gtggcgtcaa cttgatccgt tactacaagg ggtattctt
//
LOCUS       DATXQK010000004        72405 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.004, whole genome shotgun sequence.
ACCESSION   DATXQK010000004 DATXQK010000000
VERSION     DATXQK010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 72405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72405
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.004"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            161..1393
                     /locus_tag="VC394_000244"
     CDS             161..1393
                     /locus_tag="VC394_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEP5993744.1"
                     /translation="MIKRLISLVVIALFFAASTVSGEEYSVTAKHAIAVDLESGKVLY
                     EKDTKEVVPVASVSKLLTTYLVYKEVSKGKLNWDSPVTISNYPYELTTNYTISNVPLD
                     KRKYTVKELLSALVVNNANSPAIALAEKIGGTEPKFVDKMKKQLRQWGISDAKVVNST
                     GLTNHFLGANTYPNTEPDDENCFCATDLAIIARHLLLEFPEVLKLSSKSSTIFDGQTI
                     YSYNYMLKGMPCYREGVDGLFVGYSKKAGASFVATSVENQMRVITVVLNADQSHEDDL
                     AIFKTTNQLLQYLLINFQKVQLIENNKPVKTLSVLDSPEKTVKLVAQNSLFFIKPIHT
                     KTKNTVHITKKSSTMIAPLSKGQVLGRATLQDKHLIGQGYLDTPPSINLILQKNISKS
                     FFLKVWWNRFVRYVNTSL"
     gene            1561..2742
                     /locus_tag="VC394_000245"
     CDS             1561..2742
                     /locus_tag="VC394_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEP5993745.1"
                     /translation="MTKRIILTIFTFICFSVMPLVQAEDVMDITRQAGYTVSEVNRPK
                     SSIVVDANSSDILWQDNIDIPRDPASMSKMFTLYILFEELAKGKITMDTTITATPTDQ
                     AIANIYEISNNNIVAGVAYPIRDLITMTAVPSSNAATVMIANYLSNNDASAFIDRINA
                     TAKQLGMTNTHFSNASGAAAQAFQGYYNPTKYDLSASNITTARDLSKLLYAFLKKYPE
                     IISFTNKSVVHTMVGTPYEEEFHTYNHSLPDNQFGMKGVDGLKTGSSPSAAFNAMITA
                     KRGKTRLITIVMGVGDWSDQNGEFYRHPFVNALTEKGFKDSKTLSKKARQKLEKLVPQ
                     TKKETSPKQQHFKATKKQSYLERVEDFMNHNHTFLLICLAIFIITILLLSLVVFAMGR
                     Q"
     gene            complement(2894..4312)
                     /gene="sufB"
                     /locus_tag="VC394_000246"
     CDS             complement(2894..4312)
                     /gene="sufB"
                     /locus_tag="VC394_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEP5993746.1"
                     /translation="MSDINEKVEPKPIDLGDYQFGFHDDVEPIYSTGKGLSEAVVREL
                     SAAKNEPEWMLEFRLKSLETFNKMPMQTWGADLSDINFDDIIYYQKASDKPARSWDDV
                     PEKIKETFDRIGIPEAERAYLAGASAQYESEVVYHNMKGEFEKLGIIFTDTDSALKEY
                     PDLFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENTGQ
                     FERTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVY
                     NLVTKRARALTDATVEWIDGNLGAKTTMKYPSVYLDGPGARGTMLSIAFANAGQHQDT
                     GAKMIHNAPHTSSSIVSKSIAKSGGKVDYRGQVTFNKQSKKSVSHIECDTILMDDISK
                     SDTIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKE
                     LPMEYAVELNRLISYEMEGSVG"
     gene            complement(4305..4784)
                     /locus_tag="VC394_000247"
     CDS             complement(4305..4784)
                     /locus_tag="VC394_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEP5993747.1"
                     /translation="MALSKLNHLYMAVVADHSKRPHHHGQLDGVEAVQLNNPTCGDVI
                     SLTVKFDEDKIEDIAFAGNGCTISTASSSMMTDAVIGKSKEEALALADIFSEMVQGQE
                     NPAQKELGEAELLAGVAKFPQRIKCSTLAWNALKEAIKRSANAQHLTDQNVKEGKNV"
     gene            complement(4771..5997)
                     /locus_tag="VC394_000248"
     CDS             complement(4771..5997)
                     /locus_tag="VC394_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEP5993748.1"
                     /translation="MLDAKDIKQDFQILNQQVNDEPLVYLDNAATTQKPALVLEALQS
                     YYQEDNANVHRGVHTLAERATHKYEASRQQVADFIHAKSSKEVLFTRGTTTSLNWVAR
                     FAEQVLTPEDEVLISIMEHHANIIPWQQACQKTGARLVYVYLKDGQLDMDDLANKLTT
                     KTRFVSLVHVSNVLGCINPIKEIAKLAHAKGAYLVVDGAQSVPHLAIDVQDLDCDFFA
                     FSAHKMLGPTGLGVLYGKEELLNQVEPLEFGGEMIDFVYEQEATWKELPWKFEAGTPH
                     IAGAIGLSAAISYLQRLGMADIHAHEAELIAYVLPKLEAIEGLTIYGPSQPSARSGLI
                     SFNLDDLHPHDLATALDYEGVAVRAGHHCAQPLLSYLGVPATVRASFYIYNTKADCDR
                     LVEAILKAKEFFNGTL"
     gene            complement(6028..7290)
                     /gene="sufD"
                     /locus_tag="VC394_000249"
     CDS             complement(6028..7290)
                     /gene="sufD"
                     /locus_tag="VC394_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEP5993749.1"
                     /translation="MTKEKLVAFSQAHAEPAWLQERRLAALEAIPELELPTIERVKFH
                     RWNLGDGTLTENESLASVPDFIAIGDNPKLVQVGTQTVLEQLPMALIDKGVVFSDFYT
                     ALEEIPEVIEAHFGQALAFDEDKLAAYHTAYFNSAAVLYVPDHLEITTPIEAIFLQDS
                     DSDVPFNKHVLVIAGKESKFTYLERFESIGNATQKTSANISVEVIAQAGSQIKFSAID
                     RLGPSVTTYISRRGRLEKDANIDWALAVMNEGNVIADFDSDLIGQGSQADLKVVAASS
                     GRQVQGIDTRVTNYGQRTVGHILQHGVILERGTLTFNGIGHILKGAKGADAQQESRVL
                     MLSDQARADANPILLIDENEVTAGHAASIGQVDPEDMYYLMSRGLDQETAERLVIRGF
                     LGAVIAEIPIPSVHQEIIKVLDEKLLNR"
     gene            complement(7385..8155)
                     /gene="sufC"
                     /locus_tag="VC394_000250"
     CDS             complement(7385..8155)
                     /gene="sufC"
                     /locus_tag="VC394_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEP5993750.1"
                     /translation="MSILEINNLHVSIEGKEILKGVNLTLKTGEVAAIMGPNGTGKST
                     LSAAIMGNPNYEVTQGQILLDGVNILDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAMNAGKADKDKISVRDFITKLDEKMALLGMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPKFALLDEIDSGLDIDALKVVSKGVNEMRGKDFGAMIITHYQRLLNYITPDLVHV
                     MMDGRIVLSGDAALATRLEKEGYAGIAQDLGIEYKEES"
     gene            complement(8277..9446)
                     /locus_tag="VC394_000251"
     CDS             complement(8277..9446)
                     /locus_tag="VC394_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEP5993751.1"
                     /translation="MFSFTIDYVLVLIGALLMSLFLTPLVRFLAFRVGAVDNPNARRV
                     NKVPMPTSGGLAIFMSFLVASLGLIPIASKGAMFFGQTYFSYILPVVIGATVITLTGF
                     LDDLYELSPKLKMFGILIGAVIVWAFTDFKFDSFKIPFGGPLLVFGPFLTLFLTVLWI
                     VSITNAINLIDGLDGLVSGVSIISLVTMAIVSYFFLPQKDFFLTLTILVLISAIAGFF
                     PYNYHPAMIYLGDTGALFIGFMIGVLSLQGLKNSTAVAVVTPVIILGVPIMDTIVAII
                     RRSLSGQKFYEPDKMHLHHRLLSMGFTHRGAVLVVYGITMLFSLISLLLNVSSRIGGV
                     LLMLGLLFGLEVFIEGLEIWGEKRTPLFNLLKFIGNSDYRQAMLLKWKEKKDLKH"
     gene            complement(9453..10214)
                     /gene="mecA"
                     /locus_tag="VC394_000252"
     CDS             complement(9453..10214)
                     /gene="mecA"
                     /locus_tag="VC394_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEP5993752.1"
                     /translation="MEMKQISETTLKITISMDDLEERGMELKDFLIPQEKTEEFFYSV
                     MDELDLPDNFKDSGMLSFRVTPRKDRLDVFVTKSEINKDINLEDLAEFGDMSQMTPED
                     FFKSLEQSMREKGDVKAHEKLEKIEEIMEDVVEATLANQSEAADPSTNHESEPLDYVH
                     YVLDFSTITEAVAFAKTIDFSIEASELYKGSNCYHMTILLDVQQQPSYFANVMYARLI
                     EHANPGSKTRAYLQEHGLQLMLDGAVEQLQKIELG"
     gene            complement(10360..11199)
                     /locus_tag="VC394_000253"
     CDS             complement(10360..11199)
                     /locus_tag="VC394_000253"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEP5993753.1"
                     /translation="MLIIELLKAIFFGIIEGITEWLPVSSTGHLILVQEFIRLNQDKA
                     FIEMFNIVIQLGAIIAVMLIYFERLNPFQPGKTAREVQLTWQLWLKVVIACIPSILIA
                     VPLDNWFEAHFYFMVPIAIALIVYGIAFIWIEKRNAQQEPAVTELARMSYKTAFFIGC
                     FQVLSIVPGTSRSGATILGAIILGTSRTVAADFTFFLAIPTMFGYSGLKAVKFFLDGH
                     HLDFAQVLILLVASLTAFVVSLLAIRFLTDYVKKHDFTIFGKYRIVLGSLLLIYSFFK
                     FVF"
     gene            complement(11265..13190)
                     /locus_tag="VC394_000254"
     CDS             complement(11265..13190)
                     /locus_tag="VC394_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEP5993754.1"
                     /translation="MKKILMTLVLCFSLLGIRIKAADVDYSITNYEGQLLLSKENTAR
                     FEQKVTYQFDTSYNGQYISLGRAGHLPAGFAIDQKPKVEVYQNGQQVPVSQEFSDLGD
                     GYRLKLYNAGQAGDKVDVKVIWQLHHLLTAYQDVAELNWTPISDWDKTLEKVSLTVTT
                     PTDIQDSNLWAHRGYYQKKPQVLKEGNSRYQINAKNVSGQLELHAYWDKKALLGEEPV
                     DVSTSKKNKIVALETKISRRRALLQLLFGKVIPLVEVGFLLWQLIQFIRLKKQFNRYH
                     LANHTDHSYEVPEDLSPLVLTQAIYGQSFAYLSPTASESQKLLIPKGVTFEALVQATL
                     LDLIDQKVLLLTKEEGKAYLEISQLDRVTDEEAAFLDMAFGNKVTLPVDQLFSQYHYD
                     ADTIKQLKKTYKGKKLEQEVRQSSEQVIKAMKKASAAITNNVLETIKKLNLPDTYRQM
                     TPAEKRKSNSVQGLGCLLLVLNSGLLIYLAIKESGLALIYLALMVLTMCLGFYISLKL
                     DQYKKLGIETPEGGVRLHQWQSFKNMIRDIDKFEDVAIEGLVVWNRVLVYATLFGYAK
                     KVERYLKVHRIALPEVYQAVRPGELSMVMYATTPTFLSSLSSATTSSNFSVSSGGGIS
                     GGGGFSGGGGGGGGGAF"
     gene            13388..14956
                     /locus_tag="VC394_000255"
     CDS             13388..14956
                     /locus_tag="VC394_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEP5993755.1"
                     /translation="MKKLILSCLVALALLFGGMSRAQANQYLRVGMEAAYAPFNWTQD
                     DASNGAVPIEGTSQYANGYDVQVAKKVAKAMNKELLVVKTSWTGLIPALTSGKIDMIA
                     AGMSPTKERRNEISFSNSYYTSQPVLVVTANGKYADATSLKDFSGAKVTAQQGVWHVN
                     LLTQLKGAKLQTPMGDFSQMRQALTSGVIDAYISERPEAMTAEAADSRLKMITLKKGF
                     AVAESDAAIAVGMKKNDDRMATVNQVLEGFSQTDRMALMDDMVTKQPVEKKAEDAKAS
                     FLGQMWAILKGNWKQFLRGTGMTLLISMVGTITGLFIGLLIGIFRTAPKAKHKVAALG
                     QKLFGWLLTIYIEIFRGTPMIVQSMVIYYGTAQAFGISIDRTLAAIFIVSINTGAYMS
                     EIVRGGIFAVDKGQFEAATALGFTHGQTMRKIVLPQVVRNILPATGNEFVINIKDTSV
                     LNVISVVELYFSGNTVATQTYQYFQTFTIIAIIYFVLTFTVTRILRHIERRFDADTYT
                     TGANQMQIAEVSNV"
     gene            14949..15689
                     /locus_tag="VC394_000256"
     CDS             14949..15689
                     /locus_tag="VC394_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEP5993756.1"
                     /translation="MSNSIIEIKNLKKSYGSNEVLKDISLSVNKGEVISIIGSSGSGK
                     STLLRSINLLEEPSAGQILFHGEDVLAEHYNLTHYREKLGMVFQSFNLFENLNVLENA
                     IVAQTTVLKRDRAQAEQIAKENLNAVGMTEQYWQAKPKQLSGGQKQRVAIARALSVNP
                     EAMLFDEPTSALDPEMVGEVLKTMQDLAKSGLTMIIVTHEMEFARDVSDHIIFMDKGL
                     ITEEGSPQQIFENPTQDRTKEFLQRFLK"
     gene            15831..15947
                     /locus_tag="VC394_000257"
     CDS             15831..15947
                     /locus_tag="VC394_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEP5993757.1"
                     /translation="MAEKTDLSSAYRRLKSPNIKTRKRALKIIHEYKRYGKK"
     gene            complement(16173..17183)
                     /gene="gap"
                     /locus_tag="VC394_000258"
     CDS             complement(16173..17183)
                     /gene="gap"
                     /locus_tag="VC394_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEP5993758.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNIEGVEVTRINDLTDPNMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGNFIKVSAERDPENIDWATDGVEIVLEATGFFAK
                     KEAAEKHLHANGAKKVVITAPGGNDVKTVVFNTNHDILDGTETVISGASCTTNCLAPM
                     AKALHDAFGIQKGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAI
                     GLVIPELNGKLDGAAQRVPVPTGSVTELVVTLDKNVSVDEINAAMKAASNDSFGYTED
                     PIVSSDIVGVSYGSLFDATQTKVMEVDGSQLVKVVSWYDNEMSYTAQLVRTLEYFAKI
                     AK"
     gene            complement(17531..19609)
                     /gene="fusA"
                     /locus_tag="VC394_000259"
     CDS             complement(17531..19609)
                     /gene="fusA"
                     /locus_tag="VC394_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEP5993759.1"
                     /translation="MAREFSLAKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWDGHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVQTLHDRLQANAH
                     PIQLPIGAEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPEEYLEQAQEYREKLIEAV
                     AETDEDLMMKYLEGEEITNDELIAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVIA
                     YLPSPLDIPAIKGVNPDTDAEEERPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     NSGSYVMNTSKGKRERIGRILQMHANSRQEIETVYAGDIAAAVGLKDTTTGDSLTDEK
                     AKVILESIEVPEPVIQLMVEPKSKADQDKMGVALQKLAEEDPTFRVETNVETGETVIA
                     GMGELHLDVLVDRMKREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLIESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLALKEAAKSAQPAILEPMMLVTITAPEDNLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSATQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGE"
     gene            complement(19976..20446)
                     /gene="rpsG"
                     /locus_tag="VC394_000260"
     CDS             complement(19976..20446)
                     /gene="rpsG"
                     /locus_tag="VC394_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEP5993760.1"
                     /translation="MSRKNQAPKREVLPDPLYNSKIVTRLINRVMLDGKRGTAATIVY
                     DAFSAIKEATGNDALEVFETAMDNIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVNASRARGEHTMKDRLAKEIMDAANNTGASVKKREDTHKMAEANRAFAHFRW"
     gene            complement(20467..20880)
                     /gene="rpsL"
                     /locus_tag="VC394_000261"
     CDS             complement(20467..20880)
                     /gene="rpsL"
                     /locus_tag="VC394_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEP5993761.1"
                     /translation="MPTINQLVRKPRKSKIEKSDSPALNIGYNSHKKVQTKMAAPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLIRGGRVKD
                     LPGVRYHIVRGALDTAGVADRKQGRSKYGAKRPKG"
     gene            complement(21088..23709)
                     /locus_tag="VC394_000262"
     CDS             complement(21088..23709)
                     /locus_tag="VC394_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC10/PgrA surface exclusion domain-containing
                     protein"
                     /protein_id="HEP5993762.1"
                     /translation="MDLEQTKPNQVKQKIALTSTIALLSASVGVSHQVKADDRASGET
                     KASNTHDDSLPKPETIQEAKATIDAVEKTLSQQKAELTELATALTKTTAEINHLKEQQ
                     DNEQKALTSAQEIYTNTLASSEETLLAQGAEHQRELTATETELHNAQADQHSKETALS
                     EQKASISAETTRAQDLVEQVKTSEQNIAKLNAMISNPDAITKAAQTANDNTKALSSEL
                     EKAKADLENQKAKVKKQLTEELAAQKAALAEKEAELSRLKSSAPSTQDSIVGNNTMKV
                     PQGYPLEELKKLEASGYIGSASYNNYYKEHADQIIAKASPGNQLNQYQDIPADRNRFV
                     DPDNLTPEVQNELAQFAAHMINSVRRQLGLPPVTVTAGSQEFARLLSTSYKKTHGNTR
                     PSFVYGQPGVSGHYGVGPHDKTIIEDSAGASGLIRNDDNMYENIGAFNDVHTVNGIKR
                     GIYDSIKYMLFTDHLHGNTYGHAINFLRVDKRNPNAPVYLGFSTSNVGSLNEHFVMFP
                     ESNIANHQRFNKTPIKAVGSTKDYAQRVGTVSDTIAAIKGKVSSLENRLSAIHQEADI
                     MAAQAKVSQLQGKLASTLKQSDSLNLQVRQLNDTKGSLRTELLAAKAKQAQLEATRDQ
                     SLAKLASLKAALHQTEVLAEQAAARVTALVAKKAHLQYLRDFKLNPNRLQVIRERIDN
                     TKQDLAKTTSSLLNAQETLAALQAKQSSLEATIATTEHQLTLLKTLANEKGYRHLDED
                     IATVPDLQVAPPLTGVKPLSYSKIDTTPLVQEMVKETKQLLEASARLAAENTSLVAEA
                     LVGQTSEMVASNAIVSKITSSITQPSSKTSYGSGSSTTSNLISDVDESTQRALKAGVV
                     MLAAVGLTGFRFRKESK"
     gene            complement(23700..24539)
                     /gene="purR"
                     /locus_tag="VC394_000263"
     CDS             complement(23700..24539)
                     /gene="purR"
                     /locus_tag="VC394_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEP5993763.1"
                     /translation="MKLRRSERMVVISNYLINNPYKLTSLNTFATKYEAAKSSISEDI
                     AIIKKAFEEANIGDIDTLTGASGGVIFTPSISETEARTIVEDLCQRLSESDRILPGGY
                     IYLSDLLSTPKILQNIGRIIANAFKGEKIDAVMTVATKGVPLANAVANILSVPFVIVR
                     RDLKITEGSTVSVNYASASSDRIEKMFLSKRSLKPNSRVLIVDDFLKGGGTITGMISL
                     LTEFDSTLVGVAVFAENAQSEREQMTFKSLLKVSEIDVKNNNVVVEVGNIFDKWKKER
                     EWT"
     gene            complement(24806..25744)
                     /locus_tag="VC394_000264"
     CDS             complement(24806..25744)
                     /locus_tag="VC394_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEP5993764.1"
                     /translation="MKINQMKKDQLFEGFYLIKSAEVRKTRAGKDFISLTFQDDTGEI
                     SGNLWDAQPYNVEEFTAGKVVFMKGRREVYNGTPQVNQITLRNVRPGEPNDPKDFKEK
                     APVSVTEVRDYLEQMLFKIENATWQRIVRALYRKYDKEFYTYPAAKTNHHAFESGLAY
                     HTATMVRLADSIGDIYPDLNKSLLFAGIMLHDLAKVIELTGPDNTEYTVRGNLIGHIS
                     LINEEITKVISELQIDDTKEEVIVLRHVILSHHGQLEYGSPVRPRIMEAEIIHMIDNI
                     DANMMMMTTALSRVSEGEMTNRIFAMDNRSFYKPNY"
     gene            complement(25734..27005)
                     /locus_tag="VC394_000265"
     CDS             complement(25734..27005)
                     /locus_tag="VC394_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEP5993765.1"
                     /translation="MDLILFLLVLVLLGLGAYLLFKVNGLQHQLAQTLEGNADNLSDQ
                     ITYQLDTANKQQLLELTQLMNRQQADLYQQLTDIRDVLHRSLSDSRDRSDKRLEEINQ
                     QVNQSLKNMQESNEKRLEEMRQIVEEKLEETLKNRLHASFDSVSKQLESVNKGLGEMR
                     SVAQDVGTLNKVLSNTKTRGILGELQLGQIIEDIMTSSQYEREFVTVSGSSERVEYAI
                     KLPGNGQGGYIYLPIDSKFPLEDYYRLEDAYEVGDKLAIEASRKALLAAIKRFAKDIH
                     KKYLNPPETTNFGVMFLPTEGLYSEVVRNAAFFDSLRREENIVVAGPSTLSALLNSLS
                     VGFKTLNIQKNADDISKILGNVKLEFDKFGGLLAKAQKQMNTANNTLDQLISTRTNAI
                     VRALNTVETYQDQTTKSLLNMPLLEEENNEN"
     gene            complement(27007..27639)
                     /locus_tag="VC394_000266"
     CDS             complement(27007..27639)
                     /locus_tag="VC394_000266"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEP5993766.1"
                     /translation="MSKVALFAGGDLSYISRDFDYFVGIDRGSLFLLENGLPLNMAVG
                     DFDSVSQKAFTDIKEKAELFITAHPEKNDTDTELALKEVFARFPEAEVTIFGAFGGRM
                     DHLLSNIFLPSDPGIAPFMAQIALRDQQNMITYRPAGQHLIHQEEGMTYVAFMAEGEA
                     DLTITGAKFELTQDNFFKKKIYSSNAFIHQPITVSLPSGYLIIIQSKDWS"
     gene            complement(27632..28294)
                     /locus_tag="VC394_000267"
     CDS             complement(27632..28294)
                     /locus_tag="VC394_000267"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEP5993767.1"
                     /translation="MSTLKIAPSILAADYANFASELARIEETDAEYVHIDIMDGQFVP
                     NISFGADVVASMRKHSKLVFDCHLMVVDPERYVEAFAQAGVDIMTIHTESTRHIHGAL
                     QKIKAAGMKAGVVINPGTPATALEPLLDLVDQVLIMTVNPGFGGQAFIPECLEKVATV
                     AKWRDEKGLSFDIEVDGGVDNKTIRACYEAGANVFVAGSYLFKASDLVSQVQTLRTAL
                     NV"
     gene            complement(28304..29176)
                     /gene="rsgA"
                     /locus_tag="VC394_000268"
     CDS             complement(28304..29176)
                     /gene="rsgA"
                     /locus_tag="VC394_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEP5993768.1"
                     /translation="MQGKIIKSLAGFYYVESEGQVYQTRARGNFRKRGETPYVGDIVD
                     FSAEDNSEGYILAIHPRKNSLVRPPIVNIDQAVVIMSAKEPEFNSNLLDRFLILLEHK
                     AIHPVVYISKMDLLDSPEEIKAIGRQYQAIGYDFVTSLEELLPLLADKITVFMGQTGV
                     GKSTLLNRIAPELALETGEISDSLGRGRHTTRAVSFYNTHGGKIADTPGFSSLDYDIA
                     NAEDLNEAFPELRRLSHECKFRSCTHTHEPKCAVKAALETGELWPVRYEHYLQFLSEI
                     ENRRETYKKVIKRK"
     gene            complement(29600..30472)
                     /gene="rsmA"
                     /locus_tag="VC394_000269"
     CDS             complement(29600..30472)
                     /gene="rsmA"
                     /locus_tag="VC394_000269"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEP5993769.1"
                     /translation="MRIADYSVTKAVLDRHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DQNVNVIEIGPGIGALTEFLAENAAEVMAFEIDDRLVPILADTLRDFDNVQVVNQDIL
                     KADLQTQIKQFKNPDLPIKVVANLPYYITTPILMHLIESKIPFQEFVVMMQREVADRI
                     SAEPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRDQPLIEVKD
                     EDFFFRVSRLSFVHRRKTLWNNLTSHFGKSEDIKTKLEKGLALADIKPSIRGEALSIQ
                     DFGKLADALKEVGL"
     gene            complement(30586..31176)
                     /gene="rnmV"
                     /locus_tag="VC394_000270"
     CDS             complement(30586..31176)
                     /gene="rnmV"
                     /locus_tag="VC394_000270"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEP5993770.1"
                     /translation="MQRGYLSLTEKINIQEVLVVEGKDDTANLRRFYEVDTYETRGSA
                     ITEEDLERINRLNDLRGVIVLTDPDYNGERIRKLIMAAVPTARHAFLNRNEAVPSSKS
                     KGRSLGVEHANFEDLQKALAHVTQQYDDESYFDIRQTDLIRLGLLMASDSRKRREYLG
                     EKLRIGYANGKQLLKRLELFGITLAEVEEVMETYEE"
     gene            complement(31148..31939)
                     /locus_tag="VC394_000271"
     CDS             complement(31148..31939)
                     /locus_tag="VC394_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEP5993771.1"
                     /translation="MTNKVEKLTIFDTHTHLNVAEFQGHETEELTLAQEMGVAYHNVV
                     GFDQATISGALALADQYPNVYATIGWHPTEAGSYSEAVEESIVSQLSHPKVIALGEIG
                     LDYYWMEDPKEVQIEVFKRQMQLAKDHDLPFVVHTRDALEDTYEVIKAAGVGPRGGIM
                     HSYSGSLEMAERFIELGMMISFSGVVTFKKALDIQEAAQHLPLDKILVETDAPYLTPV
                     PKRGKQNHTAYTRYVVDKIAELRGMTVEEVAKETTANAKRVLKLD"
     gene            32224..33054
                     /locus_tag="VC394_000272"
     CDS             32224..33054
                     /locus_tag="VC394_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEP5993772.1"
                     /translation="MPKNHRLITKIEASLEHMTSLEKGIAHFFITTDLTPQELTASEI
                     VKRLHISQAALTRFAKKCGFTGYRAFAFDYLHSLQESQETFQSIHLELTKRVLMDYDA
                     LINKTYELVNEEKLLNLAKLIDASERVYFFGKGSSGLVAREMKLRFMRLGLICDAYSD
                     TDGFTWANSLVNENCLVFGFSLSGKTNSVITALHQASQRGAKTVLLTTDNQTEFDDSL
                     DIIPVSSTHQLHYGNRVSPQFPLLIMMDIIYAYVLAIDKPHKEKIFKNTIIDKENNGL
                     "
     gene            complement(33165..34103)
                     /locus_tag="VC394_000273"
     CDS             complement(33165..34103)
                     /locus_tag="VC394_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEP5993773.1"
                     /translation="MKHYLAIDIGGTAIKYGLISETGDLLEKEEMATEAYKGGPSILE
                     KVKGLVKTYQDQMDLAGVAISSAGMVNPDKGEIFYAGPQIPNYAGTQFKKEIEETFGL
                     PCEVENDVNCAGLAEAISGSAKDYPVALCLTIGTGIGGCLLFNSQVFHGSSHSACEVG
                     YLHLSDGQFQDLASTTALVQEVALAYGDDISQWDGRRIFEQAKAGDAICIAAISKQVD
                     YLGQGIANICYVVNPNVVVLGGGIMVQKDYLADKLKTALDSYLVSSLAKKTQLKFASH
                     GNNAGILGAYYHFKQKNERSCSFITLEKIENNLASS"
     gene            complement(34125..35039)
                     /locus_tag="VC394_000274"
     CDS             complement(34125..35039)
                     /locus_tag="VC394_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEP5993774.1"
                     /translation="MTDLTKYQGIIPAFYACYDDQGAISPERVRALTQYYIDKGVQGL
                     YINGSSGECIYQSVFDRQLVLENVMAVAKGKLTIINHVACNNTKDSIELAAHSERLGV
                     DAIAAIPPIYFRLPEYAVADYWNAISSAAPHTDFIIYNIPQLAGVALTPSLYKTMLAN
                     KRVIGVKNSSMPVQDIQTFCAIGGDDHIVFNGPDEQFLGGRLMGAAAGIGGTYGAMPE
                     LFLRLNQLIVDKDLEKAKTLQYTINETIGVLVSAHGNMYGVIKEVLRINEGLDIGSVR
                     SPLAELVEEDRVICQRAAALINQAKETF"
     gene            complement(35051..35713)
                     /locus_tag="VC394_000275"
     CDS             complement(35051..35713)
                     /locus_tag="VC394_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEP5993775.1"
                     /translation="MTSKKQGLLHSLFKLDSKWMRASAALFDLLVFNLLFVLSCLPLL
                     TIGVAKMALYASLLDWREGQVSQLVTTYSSHFKHYFKSGMRLGLIELLIMSICVLDLF
                     LIRNQSGLVFQGFKVLCIAVLFLVVILFLYAYPQAVRKELPLLILFKRSLLLAGILFP
                     WTFAFLVVIGLLVFTLPLTLLTLFCGLSLLATMGISSLTYLYLIIMESLLRRFPLNND
                     IE"
     gene            complement(35870..36700)
                     /locus_tag="VC394_000276"
     CDS             complement(35870..36700)
                     /locus_tag="VC394_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEP5993776.1"
                     /translation="MTKKKLTASDVLTTVILCILTILFIFPFYWIMTGAFKAQADTIM
                     IPPQWWPKAPTIENFKALVVQNPALKWLWNSVFISVATMFLVCGTSSLAGYALAKKRF
                     YGQRLLFSIFIAAMALPKQVVLVPLVRIVNFMGIHDTLAAVILPLVGWPFGVFLMKQF
                     SENIPTELLESAKIDGCGEIRTFFNVAFPIVKPGFAALAIFTFINTWNDYFMQLVMLT
                     SRENLTISLGVATMQAEMATNYGLIMAGAAMAAVPIVTVFLVFQKSFTQGITMGAVKG
                     "
     gene            complement(36713..37600)
                     /locus_tag="VC394_000277"
     CDS             complement(36713..37600)
                     /locus_tag="VC394_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001260031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEP5993777.1"
                     /translation="MNVNKLKMRETLISYAFLAPVLVFFVIFVLIPMIMGFVTSFFNY
                     SMTDFTFVGFANYARMFQDPIFMKSLINTLIIVIGSVPVVVFFSLFVAAKTYDKNVVA
                     RSFYRAVFFLPVVTGSVAVTVVWKWIYDPMSGILNYVLKYAHVIEQNISWLGDKHWAL
                     LAIIAILLTTSVGQPIILYIAAMGNIDNSLMEAARVDGATEFQVFWNIKWPSLLPTTL
                     YIAVITTINSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFKLSEYGYANTMGVFLAV
                     MIAIISFAQFKILGNDVEY"
     gene            complement(37703..39022)
                     /locus_tag="VC394_000278"
     CDS             complement(37703..39022)
                     /locus_tag="VC394_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEP5993778.1"
                     /translation="MNMKKLASLVMLGASVLGLAACGGKSQKEAGASKSDTAKTEITW
                     WAFPVFTQEKAEDGVGTYEKKLIAAFEKANPEIKVKLETIDFTSGPEKITTAIEAGTA
                     PDVLFDAPGRIIQYGKNGKLADLNDLFTEEFTKDVNNDKLIQASKAGDTAYMYPISSA
                     PFYMALNKKMLKDAGVLDLVKEGWTTDDFEKVLKALKDKGYNPGSFFANGQGGDQGPR
                     AFFANLYSSHITDDKVTKYTTDDANSIKAMTKISNWIKDGLMMNGSQYDGSADIQNFA
                     NGQTSFTILWAPAQPGIQAKLLEASKVDYLEIPFPSDDGKPELEYLVNGFAVFNNKDE
                     QKVAASKTFIQFIADDKEWGPKNVVRTGAFPVRTSYGDLYKDKRMEKIAEWTKFYSPY
                     YNTIDGFAEMRTLWFPMVQAVSNGDEKPEDALKAFTEKANKTIKKTQ"
     gene            complement(39071..39775)
                     /locus_tag="VC394_000279"
     CDS             complement(39071..39775)
                     /locus_tag="VC394_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEP5993779.1"
                     /translation="MPDKPTKEKLMEQLKGGIIVSCQALPGEPLYSETGGIMPLLAKA
                     AQEAGAVGIRANSVRDIKEIQAITDLPIIGIIKKDYPPQEPFITATMAEVDQLAALNI
                     AVIAMDCTKRDRHDGLDIASFIRQVKEKYPNQLLMADISTFDEGLVAHQAGIDFVGTT
                     LSGYTPYSRQEAGPDVALIEALCKAGIAVIAEGKIHSPEEAKKINDLGVAGIVVGGAI
                     TRPKEIAERFIEALKS"
     gene            complement(40049..40183)
                     /gene="rpmH"
                     /locus_tag="VC394_000280"
     CDS             complement(40049..40183)
                     /gene="rpmH"
                     /locus_tag="VC394_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEP5993780.1"
                     /translation="MKRTYQPSKIRRQRKHGFRHRMSTKNGRRVLAARRRKGRKVLSA
                     "
     gene            complement(40498..41412)
                     /locus_tag="VC394_000281"
     CDS             complement(40498..41412)
                     /locus_tag="VC394_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEP5993781.1"
                     /translation="MVLFTGKTVEEAIETGLQELGLSRLKAHIKVISKEKKGFLGFGK
                     KPAQVDIEGISDKTVYKADKKATRGVPEDINQKNAPAVNSADVEPEEIKATQKLEAED
                     TKVVPLMSEDSPAQTLSNLAETVTETKAQQPSIPVEESEVPQDPGNDGFSKDIEKAAQ
                     EVSDYVTKIIYEMDIEATVETSNNRRQINLQIETPEAGRVIGYHGKVLKSLQLLAQNF
                     LHDRYSKNFSVSLNVHDYVEHRTETLIDFTQKVAKRVLESGQDYTMDPMSNSERKIVH
                     KTVSSIEGVDSYSEGNDPNRYVVVSLQR"
     gene            complement(41424..42251)
                     /locus_tag="VC394_000282"
     CDS             complement(41424..42251)
                     /locus_tag="VC394_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEP5993782.1"
                     /translation="MRKVLRVKKNIKIARIVPLVLLLVACGRGEVTAQSSSGWDQLVY
                     LFARAIQWLSFDGSIGVGIILFTLTIRLMLMPLFNMQIKSSQKMQDIQPELRELQKKY
                     AGKDTQTRMKLAEESQALYKKYGVNPYASLLPLLIQMPVMIALFQALTRVSFLKTGTF
                     LWVELAQHDHLYLLPVLAAVFTFLSTWLTNLAAKEKNVMMTVMIYVMPLMIFFMGFNL
                     ASGVVLYWTVSNAFQVVQLLLLNNPFKIIAERQRLANEEKERRLRERRARKKAMKRK"
     gene            complement(42217..42576)
                     /gene="rnpA"
                     /locus_tag="VC394_000283"
     CDS             complement(42217..42576)
                     /gene="rnpA"
                     /locus_tag="VC394_000283"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEP5993783.1"
                     /translation="MKKTYRVKREKDFQAIFKDGKSTANRKFVIYHLNRGQDHFRVGI
                     SVGKKIGNAVTRNAVKRKIRHVIMALGHQLKSEDFVVIARKGVESLEYQELQQNLHHV
                     LKLAQLLEKGFESEEKH"
     gene            complement(43116..43856)
                     /locus_tag="VC394_000284"
     CDS             complement(43116..43856)
                     /locus_tag="VC394_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP5993784.1"
                     /translation="MMNIFILEDDFIQQTRIESIVVGILKETRIPCNQLEVFSTPQKL
                     FESIQERGDHQLYFLDIEIGEYTRCGLELAAAIRQKDPNAVIVFVTTHSEFAPISFKY
                     KVSALDFIDKAGGQKQFKEQIEECIRYTYDMMSSRESKDMFLFETPQTRLKLPYKDIL
                     YFATATTPHKVCLWTQTERLEFYGNLSEIQAVAPKLFLCHRSYLVNLDKVVRIDKSKQ
                     LLYFENGDSCMVSRLKMKCLFERWEALR"
     gene            complement(43860..45143)
                     /locus_tag="VC394_000285"
     CDS             complement(43860..45143)
                     /locus_tag="VC394_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEP5993785.1"
                     /translation="MMVIYSMALFYATIFINSWVIFAKVSAIKLSWKRVSVIGISFVI
                     ANMIFDKVILIDQLFFIIVSLLSAPKKKLFEHMFNGFFTILIVELLFRVIGSFFLPAV
                     LGFSIGQINNNLKLLELCYLFVLPIFYLFSYIFSIDLSLIRFISEDKMKKWVFWMNTA
                     MFSYYFFAHFLVTVQSGFLALYFQYRSILVFIYLAIFIWVIVKLDRFAKDQLSQKLTQ
                     AQNERIAYLENYNQSIEQLYREIRTVKHDSENILISLKDSIDSGDIDLITRVYDTVIQ
                     QSATSMMRTNYEISSLDNIKEAVIRSIMNSKLLEAQYLGIELYIEIPDVIDHLPIKLI
                     DLIVLFTGLVDNAIETAKGSRRPFLSIAYFKQDNKQLFIIENSTKTNRVDIAKRFDAQ
                     QQNSAHFLTVLDSYPQITLSTKSDHYRLRQLLEMR"
     gene            complement(45140..46486)
                     /locus_tag="VC394_000286"
     CDS             complement(45140..46486)
                     /locus_tag="VC394_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEP5993786.1"
                     /translation="MANSEYMFLLLSIIVYYMTKIYIFSFLSDITLPVWKQLTILALA
                     LFFNQFPYLSPLLIDPLLFLVVLRQETKQLFSLKALFLAVAPSVLVDLLSRFMGTIVI
                     PYLFLSSGIYLGHIIFDLLAYLLIFPSFAIINYMIGKDYKMICQSGYSKRSHNFYQTL
                     LIFVLVYYVDIFVILGFTDPFLHFHHSLFVPTPYKLLFLMFILLLVYLLSYFNHSSKE
                     YLKNELRREQQAYMTNLETYGKHLEKLYRDVRAFQSDYLSRIERLGQAIKSESITQIQ
                     DIYAQTVHEANDYWDDKHYNISKLRKINISSIKSLLSAKIISAEKSGIDLNVEVPDNI
                     KETYIPELDLLLLMSIFCDNAIEAALEAQQPHMSIAYFLLDDYQMFVVTNTTKKKVDI
                     NKIFEEGYSSKGSERGIGLSNAQRILKKYPYLSLRTKSFDKEFSQTLTMPKEEVDR"
     gene            complement(46881..48326)
                     /locus_tag="VC394_000287"
     CDS             complement(46881..48326)
                     /locus_tag="VC394_000287"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEP5993787.1"
                     /translation="MSKPIRVRYAPSPTGLLHIGNARTALFNYLYARRHGGTFIIRIE
                     DTDRKRHVEDGERSQLENLKWLGMDWDESPETHENYRQSERLALYQQYIDQLLAEGKA
                     YKSYVTEEELAAERERQEAAGETPRYINEFIGMSADEKAKYIAEREAAGIVPTVRLAV
                     NESGIYKWTDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVVDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYM
                     PEAVFNFIALLGWNPGGEEEIFSREQLIALFDENRLSKSPAAFDQKKMDWMSNEYLKH
                     ADFETVYALCKPFLEEAGRLTEKAEKLVELYKPQLKSADEIIPLTDLFFSDFPELTEA
                     EKEVMAGETVSTVLQAFKAKLEAMSDEDFKPENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPNTIYLLGRDKSIEHIKNML"
     gene            complement(48509..49219)
                     /locus_tag="VC394_000288"
     CDS             complement(48509..49219)
                     /locus_tag="VC394_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HEP5993788.1"
                     /translation="MVDNRMRFTIDQNMQFPLVEIDLEHGGSVYLQQGSMVYHTENVT
                     LNTKLNGKGSGLGKLVGAIGRSMVSGESMFITQAMSNGDGKLALAPNTPGQIVALELG
                     EKQYRLNDGAFLALDGSAQYKMERQNIGKALFGGQGGLFVMTTEGLGTLLANSFGSIK
                     KITLDGGTMTIDNAHVVAWSRELDYDIHLENGFMQSIGTGEGVVNTFRGHGEIYIQSL
                     NLEQFAGTLKRYLPTSSN"
     gene            complement(49350..49847)
                     /locus_tag="VC394_000289"
     CDS             complement(49350..49847)
                     /locus_tag="VC394_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEP5993789.1"
                     /translation="MSYFEHFMAANQAYVALHGTAHLPLKPKTKVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFTNEGF
                     AKHIHEHLGVDVSGQDFLPFQDVEDSVRKDMAKLRASSLIPDDVVINGAVYDVATGKM
                     TQVME"
     gene            complement(50036..51397)
                     /gene="radA"
                     /locus_tag="VC394_000290"
     CDS             complement(50036..51397)
                     /gene="radA"
                     /locus_tag="VC394_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEP5993790.1"
                     /translation="MAKKKATFICQECGYQSPKYLGRCPNCSAWSSFVEEVEVKEVKN
                     ARVSLAGEKSRPVKLKDVDNISYHRTQTDMSEFNRVLGGGVVPGSLILIGGDPGIGKS
                     TLLLQVSTQLANKGTVLYASGEESAEQIKLRSERLGDIDNEFYLYAETNMQAIRTEIE
                     NIKPDFLIIDSIQTIMSPDITGVQGSVSQVREVTAELMQLAKTNNIATFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSAVVVTMEGSRPILAEVQSLVTPTVFGNARRTTTGLDFNRVSLI
                     MAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEKPTSPQEAFLGE
                     IGLTGEIRRVTRIEQRINEAAKLGFTKVYAPKNALQGIDIPQGIEVVGVTTVGQVLKA
                     VFS"
     gene            complement(51478..51924)
                     /locus_tag="VC394_000291"
     CDS             complement(51478..51924)
                     /locus_tag="VC394_000291"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEP5993791.1"
                     /translation="MTKIRGFELVSSFTNPDLLPKRETTHAAGYDLSVAEAVTIAPGE
                     IKLVPTGVKAYMQDGEVLYLYDRSSNPRKKGIILINSVGVIDADYYGNEANEGHIFAQ
                     MQNITDHPVTLAVGERIVQGVFMPFLIADGDQARGERTGGFGSTGQ"
     gene            complement(52034..52801)
                     /locus_tag="VC394_000292"
     CDS             complement(52034..52801)
                     /locus_tag="VC394_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEP5993792.1"
                     /translation="MIDLQEILANMNPNQKINYDRVMQQMAKVWAKESVRPSILMHVC
                     CAPCSTYTLEYLTQFADITVYFANSNIHPKDEYHRRAYVTQQFVSEFNAKTGNTVQFL
                     EADYVPNEYVRQVRGLEEEPEGGDRCRVCFDYRLDKTAQKAVELGFDYFASALTISPH
                     KNSQTINDVGIDVQKVYTTKYLPSDFKKNNGYRRSVEMCEEYDIYRQCYCGCVYAAKM
                     QGIDLVQVKKDAKAFMADKDLDNDFTHIRFSYRGDEM"
     gene            complement(52919..54703)
                     /locus_tag="VC394_000293"
     CDS             complement(52919..54703)
                     /locus_tag="VC394_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5993793.1"
                     /translation="MKTARFFWFYFKCYRFSFTVIAVAVILATYLQVKAPVFLGESLT
                     ELGKIGQAYYVAKMSGQTHFSPDLSAFNAVMFKLLMTYFFTVLANLIYSFLLTRVVSH
                     STNRMRKGLFGKLERLTVAFFDRHKDGEILSRFTSDLDNIQNSLNQSLVQVVTNIALY
                     IGLVWMMFRQDSRLALLTIASTPVALIFLVINIRLARKYTNIQQQEVSALNAFMDETI
                     SGQKAIIVQGVQEDTVTAFLKHNERVRQATFKRRLFSGQLFPVMNGMSLINTAIVIFV
                     GSTIVLSDKSMPAAAALGLVVTFVQYSQQYYQPMMQIASSWGELQLAFTGAHRIQEMF
                     DETEEVRPQNAPAFTSLKEAVAINHVDFGYLPGQKVLSDVSIVAPKGKMIAVVGPTGS
                     GKTTIMNLINRFYDVDAGSITFDGRDIRDYDLDSLRQKVGIVLQESVLFSGTITDNIR
                     FGDQTISQDMVETAARATHIHDFIMSLPKGYNTYVSDDDNVFSTGQKQLISIARTLLT
                     DPEVLILDEATSNVDTVTESKIQRAMEAIVAGRTSFVIAHRLKTILNADHIIVLKDGK
                     VIEQGNHHELLHQKGFYAELYHNQFVFE"
     gene            complement(54706..56412)
                     /locus_tag="VC394_000294"
     CDS             complement(54706..56412)
                     /locus_tag="VC394_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5993794.1"
                     /translation="MLKDAILRYKWYALGSFFMVIGVVASSLLQPHYLKSVLSAVIQN
                     NQEKIYEVGKPLLIIALLGLMSGAINTVLAAKIAQGVSADMREKTFRKIQDFSYANIE
                     AFNAGNLVVRLTNDINQIQSLVMMMFQILFRLPILFIGAFIMAVQTFPQLWWVIVVMV
                     ILIALIMGLVMRQMGPRFGKFQRLMDKINRIAKENLRGVRVVKSFVQEQQQYTKFKET
                     SNDLLALNLSIGYGFSLMQPALMLVSYLAVYVSINVVSTMVETDPTVIGNIASFMTYM
                     MQIMFSIIVVGSMGMQVSRAFVSMARIRQILSTEPAMTFENEKEETISGSIVFDDVSF
                     TYPNDDEPTLKHISFAIESGQMVGIVGATGSGKSTLAQLIPRLFDPQDGQILLGGKPI
                     KTLSQTTLRQSVSIVLQKAILFSGTIADNLRQGSAKADIDAMQKAAQIAQAKEFIDRM
                     DSRYESQVEERGSNLSGGQKQRLSIARGVINHPKILILDDSTSALDAKSEKRVQEALS
                     HKLEGTTTVIVAQKISSVVKADKILVLDQGQLIGEGTHAELVANNAIYREIYETQKGR
                     EE"
     gene            complement(56405..56854)
                     /locus_tag="VC394_000295"
     CDS             complement(56405..56854)
                     /locus_tag="VC394_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEP5993795.1"
                     /translation="MSQVIGDLRELIHQIEQISDEIAKKYDVEHLAGPQGYVLVFLAK
                     HQNQEIFVKDIEKQLRISKSVASHLVKRMVKNGFINVMPSQVDKRYKQVVLAQVGRDK
                     LPLLRECRKDIEHYFLKEITKEELLTAKKVIEQLKQNMLTYKGDNDA"
     gene            57152..58168
                     /locus_tag="VC394_000296"
     CDS             57152..58168
                     /locus_tag="VC394_000296"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEP5993796.1"
                     /translation="MTKQKVAILGPGSWGTALSQVLNDNGHDVRLWGNIPDQIEEINT
                     KHTNRHYFKDIVLDKNITATLDLGQALSDVDAVLFVVPTKVTRLVARQVAAILDHKVV
                     VMHASKGLEPETHERLSTILEEEIPAHFRSEVVVVSGPSHAEETIVRDITLITAASKD
                     IEAAKYVQSLFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGYGDNAKAAVI
                     TRGLAEITRLGVKLGADPLTYSGLSGVGDLIVTGTSVHSRNWRAGAALGRGEKLEDIE
                     HNMGMVIEGIATTKVAYEIAQDLGVYMPITTAIYKSIYEGADIKESILGMMSNEFRSE
                     NEWH"
     gene            58201..59100
                     /gene="galU"
                     /locus_tag="VC394_000297"
     CDS             58201..59100
                     /gene="galU"
                     /locus_tag="VC394_000297"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEP5993797.1"
                     /translation="MTKVRKAIIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVEE
                     ALKSGIEEILIVTGKSKRSIEDHFDSNFELEYNLQAKGKIELLKLVDETTSINLHFIR
                     QSHPRGLGDAVLQAKTFVGNEPFVVMLGDDLMDITNPNVKPLTKQLIDDYEETHAATI
                     AVMRVPHEDVSNYGIIAPQAKAVKGLYSVDTFVEKPQPQDAPSDLAIIGRYLLTPEIF
                     SILEKQEPGAGNEVQLTDAIDTLNKTQRVFAREFKGKRYDVGDKFGFMKTSLDYALKH
                     PQVKDDLKAYIIQLGKALEKTKP"
     gene            complement(59199..59870)
                     /locus_tag="VC394_000298"
     CDS             complement(59199..59870)
                     /locus_tag="VC394_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEP5993798.1"
                     /translation="MTRLLKRYPITIFLLGLTGLIFIAMQVVYGHLATGAQAIYQVGG
                     MFGLLVKAMPDQLWRLVTPIFIHIGFGHFFVNGLTLYFVGQIVEDLWGSRRFLLLYVL
                     SGVMGNAFTFWLSPETVAAGASTSLFGLFAAIVVLSFLGKNQALKDLGKSYQTLIVVN
                     LLMNLFMPNVSMAGHIGGVVGGVLLSIVFPTKMRVITVKKTKRMLALVSYGIILVGVL
                     VLGFL"
     gene            60558..62066
                     /locus_tag="VC394_000299"
     CDS             60558..62066
                     /locus_tag="VC394_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEP5993799.1"
                     /translation="MLDYYLESDIIDQKILLAILLTEKELTMAAACSQTTLTALKIKQ
                     YLRQFNALFKGYLRIELIKSRIYCEVLNDKREAFFYDIFALSDTLKVLTFLLLDNPKH
                     KSIAVYTRKQGISQSKAYRLIHKLKHYLQDIGLNIVDNTVIGDELKIRYLIALLHKEY
                     GIILYDIQPADIETIHAFIFAAQKNLQPSAFLDRRFLFFDVLLMLTWKRHRYPVHLPH
                     LALFEHLKSLPIFDNIKTIAVDELAPRTRVTFSSDDFDYLFLIYLTTDNSFLSGYWTS
                     HQRQQLYHLITKDPDYHLLIHRLQALVGKYSDIHEHIPNLIPFFKRTLYNLQTLITFD
                     GYYFDQYQGNMLLLDKLETVIKDWLYDTGRQGSISSGHLHLMCLYLEQILESSIAPIN
                     ITVIESQETVGNVIGNFITSTIPSYKVELSRVNILSDNIYPYDKPVDLVVTSQKLLPF
                     LKELGVFPKETRLFGLSLDCIQQQREDLIKTILALHQNHYQKRLEELWRVPS"
     gene            complement(62415..63764)
                     /locus_tag="VC394_000300"
     CDS             complement(62415..63764)
                     /locus_tag="VC394_000300"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEP5993800.1"
                     /translation="MSHITFDYSKVLESFAGQHEIDFLQGQVTEADKLLREGTGPGSD
                     FLGWLDLPENYDKEEFARILTAAEKIKADSEVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTAKERKAPQILYAGNSISSTYLADLVEYVQDKEFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDKVKGAVKLEADANNWETFVVPDNVGGRFSVLTAVGLL
                     PIAASGADITALMEGANAARKDLSSDKISENIAYQYAAVRNVLYRKGYITEILANYEP
                     SLQYFGEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGYRNLFETVIRVDN
                     PRKNVIIPELAEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGGVPNMFVTLPAQDEF
                     TLGYTIYFFELAIAVSGYMNAVNPFDQPGVEAYKRNMFALLGKPGFEALSAELNARL"
     gene            complement(64220..64924)
                     /gene="speG"
                     /locus_tag="VC394_000301"
     CDS             complement(64220..64924)
                     /gene="speG"
                     /locus_tag="VC394_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptococcal pyrogenic exotoxin SpeG"
                     /protein_id="HEP5993801.1"
                     /translation="MKTNILTIIILSCVFSYGSQLAYADENLKDLKRSLRFAYNITPC
                     DYENVEIAFVTTNSIHINTKQKRSECILYVDSIVSLGITDQFIKGDKVDVFGLPYNFS
                     PPYVDNIYGGIVKHSNQGNKSLQFVGILNQDGKETYLPSEAVRIKKKQFTLQEFDFKI
                     RKFLMEKYNIYDSESRYTSGSLFLATKDSKHYEVDLFNKDDKLLSRDSFFKRYKDNKI
                     FNSEEISHFDIYLKTH"
     gene            complement(65026..65124)
                     /gene="ffs"
                     /locus_tag="VC394_000302"
     ncRNA           complement(65026..65124)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VC394_000302"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            65324..66556
                     /locus_tag="VC394_000303"
     CDS             65324..66556
                     /locus_tag="VC394_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein"
                     /protein_id="HEP5993802.1"
                     /translation="MGVMMKQKIKILTVIGLMTVGMSACHNTSKPSNTDSVFSLTGKK
                     RQQIVKQVRQRYYFQQLSKTEQENYLTLYDSLAQFREIISLTPASKKSLIKTIDAFVM
                     DNPEFYWITSADYRFEFSDQTVFVTFPIPEDAKNVYQDLQAIGNDIVANTPSKDRYEQ
                     VKYFYEVIIRDTDYNKKAFEAYQSGSQAQVASNQDIKSVFIDHLSVCNGYAQAFQFLC
                     QKAGIPVAYIRGTGTSQQPQQSFAHAWNAVQINNTYYGVDVTWGDPVFDNHLSHQKQG
                     TINYSFLCLPDYLMALSHQPSKDIAFNTKERFENVWTIPSCTDDSLLYSKRHQSYIST
                     FDSDAILASLENQLLNRQEQLCLQFAHQDDYQQMVTDLTTNQTGYHNLFNQYWNNYTG
                     FTYGLLPETLSISFASRN"
     gene            complement(67170..67685)
                     /locus_tag="VC394_000304"
     CDS             complement(67170..67685)
                     /locus_tag="VC394_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEP5993803.1"
                     /translation="MPYSLEEQTYFMQEALKEAEKSLQKAEIPIGCVIVKDGEIIGRG
                     HNAREESNQAIMHAEMMAINEANAHEGNWRLLDTTLFVTIEPCVMCSGAIGLARIPHV
                     IYGASNQKFGGADSLYQILTDERLNHRVQVERGLLAADCANIMQTFFRQGRERKKIAK
                     HLIKEQSDPFD"
     gene            complement(67685..68464)
                     /locus_tag="VC394_000305"
     CDS             complement(67685..68464)
                     /locus_tag="VC394_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEP5993804.1"
                     /translation="MKLTTLGSWGAYPYQDAGTTSYLVTGHDGFQLLMDAGSRALNEL
                     EKEISPLDLDAVIISHYHPDHVADLGVLRHYFQLYPKHLWQPKVLPIYGHDEDRHEFA
                     KLSIPDVSEGRAYDVTGVEQIGPFDITFLKTVHPVVCYAFRIVERATGQVLVFTGDSG
                     YFEGLADFAKEADLFLADVYLYEGNENHMAHLTSKEAGLMASQAGVKKLVLTHMPPVP
                     PEGIDPDNHLEVLRQETQAYAGNIPVDLALPHKSWNLGSLS"
     gene            complement(68607..69143)
                     /locus_tag="VC394_000306"
     CDS             complement(68607..69143)
                     /locus_tag="VC394_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEP5993805.1"
                     /translation="MFTTKELVKVAMMTTLIIILGFIPAIPLGFIPVPIVLQNLGVML
                     AGLMLGGKKGTLSVFLFLVIGLFLPVFSGSRTTIPVLMGPSAGYVIAYLLVPIVFSLL
                     YRNWFSKSTPLAFLALLISGVVLVDVLGAIWLAAYTGMSLVTSLLSNLVFIPGDTIKA
                     IIATIIAVKYKDSFLNTK"
     gene            complement(69147..69458)
                     /locus_tag="VC394_000307"
     CDS             complement(69147..69458)
                     /locus_tag="VC394_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5993806.1"
                     /translation="MTDCFGIDLDQEYRCLHYHTPLDIVGLKCAFCQTYYACYHCHDQ
                     LTDHAFVPTGHQETSPVICGHCRKLLSLAEYGCGCCPYCQSPFNPACHRHKDIYFLKE
                     S"
     gene            complement(69675..70817)
                     /gene="tgt"
                     /locus_tag="VC394_000308"
     CDS             complement(69675..70817)
                     /gene="tgt"
                     /locus_tag="VC394_000308"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEP5993807.1"
                     /translation="MTDYPIKYRLIKTEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKAIGSGIILSNTYHLWLRPGDELIARSGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAADLVAMDFPGYSI
                     GGLAVGESHEEMNAVLDFTTPLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSEGRLVIKNAKFAEDFTPLDHDCDCYTCQNYSRAYIRHLLKADETFGIR
                     LTSYHNLYFLVNLMKKVRQAIMDDNLLEFRQDFLERYGYNKSNRNF"
     gene            71036..71899
                     /locus_tag="VC394_000309"
     CDS             71036..71899
                     /locus_tag="VC394_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEP5993808.1"
                     /translation="MSIKAIKGQARDTLKNLSGKYLLFLIPTLLFMFHFGIEIHQGYV
                     LSSGIEVSLAASYFPLLLGVILSLFILSASFTMIDVVRHFRQKVSFAESTTAFSKEFF
                     GNLLVLAITKWLFFLIWSLIWFFGLFIFLSGLSAFLVNAKSGGSTVISLIFLLFGAVL
                     SLIGFGIYINRYYAYSLSEYLLYDKVKEGTYLGAIAVIETSVAMMKGYKWKLFFLQLS
                     FTGWFLLNIVTFGLLNIYLLPYFTTANVIFYDQLKKRFKDKDDPIEGEHLSLHQLNKK
                     PTPMMYDDQKD"
     gene            72096..>72405
                     /locus_tag="VC394_000310"
     CDS             72096..>72405
                     /locus_tag="VC394_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEP5993809.1"
                     /translation="MIRLIDRYGVTIVQKCKNNYYSPELKQEIINEVLIDGQSQKQTS
                     LDYALPTSSMLSRWIAQYKKNGYTILEKPRGRPSKMGRKRKKNLEEMTEVERLQKELE
                     Y"
BASE COUNT        22047 a        15289 c        13071 g        21998 t
ORIGIN      
        1 tatactcctt ttttaatgag gtttcaatta gtttaataaa gattgtcatt ctctcatggt
       61 agcgttttat tgcatcacta ctcttgtgag gtaagtcatt accttagaca aaaatatggt
      121 ataattaaaa aaataatact tgagtaagga taatctataa atgatcaaac gattaatttc
      181 cctagtggtc atcgccttat tttttgcagc aagcactgtt agcggtgaag agtattcggt
      241 aactgctaag catgcgattg ccgttgacct tgaaagtggc aaagttttat acgaaaaaga
      301 tactaaagaa gttgtcccag tcgcctcagt cagtaagctc ttgacaacct atctggttta
      361 caaagaagtt tctaagggca agctaaattg ggatagtcct gtaactattt ctaactaccc
      421 ttatgaactc actacaaact atactattag taacgttcct cttgataaga gaaaatatac
      481 cgttaaagaa cttttaagtg cgttagttgt taataacgcc aatagccccg ctattgcttt
      541 agctgaaaaa ataggcggaa ccgaacccaa atttgttgac aaaatgaaaa aacaattaag
      601 gcaatggggc atttccgatg caaaggtcgt taattcaact ggcttaacta accatttttt
      661 aggagctaat acttatccta atacagaacc agatgacgaa aattgttttt gcgccactga
      721 tttagctatt attgccaggc atctcttatt agaatttcca gaagtactga aattatctag
      781 caaatcctcc actatttttg atggacaaac catttacagt tataattaca tgcttaaagg
      841 catgccttgt tatcgagaag gcgtggatgg tctttttgtt ggttattcta aaaaagccgg
      901 tgcttctttt gtagctacta gtgtcgaaaa tcaaatgagg gttattacag tagttttaaa
      961 tgctgatcaa agccacgagg atgatttagc tatatttaaa acaaccaatc aattgttgca
     1021 gtacctttta attaattttc aaaaagtcca gttaattgaa aataataaac cagtaaaaac
     1081 gttatctgtc ttagacagtc ctgaaaaaac tgtcaaactt gtagcccaaa atagtttatt
     1141 ttttatcaaa ccaatacata caaagaccaa aaataccgtc catattacta agaaatcatc
     1201 cacaatgatc gcacctctat caaagggaca agtcttaggt agagcaaccc ttcaagataa
     1261 acatcttatt ggacagggtt atctggatac tcctccttct atcaatctta tccttcaaaa
     1321 aaacatttct aaaagtttct ttttaaaggt ctggtggaac cgttttgtga ggtatgtcaa
     1381 tacctcttta tagaataaca caatgctttt tgtatgacat tgactgacag agtacttctg
     1441 cagtcttttt catttttatt taagcaaact aggccaatcc taacaatttt tcaaaaaaac
     1501 ttcacattgt attagcaaag tgatatactg aaaatgcatt acatttaaag gagtcggaaa
     1561 ttgaccaaaa gaattatact aactattttt acctttattt gcttttcggt gatgcctctt
     1621 gtccaagccg aagatgtcat ggatattaca aggcaagcag gctacacagt tagtgaggtt
     1681 aatcgaccaa aatcttctat tgtcgttgat gcaaatagtt cagatattct ttggcaagat
     1741 aatattgata ttcctaggga tcctgctagt atgtctaaaa tgtttacgct ttacatttta
     1801 tttgaagagc tcgctaaggg aaaaattacg atggacacta ctattacggc aactccaacc
     1861 gatcaagcta tcgctaacat ttatgagatt agcaataata atatcgtcgc tggagtagct
     1921 tatcctattc gtgatcttat tactatgaca gctgtgcctt catcaaatgc agcaactgtt
     1981 atgattgcca attatttatc aaacaatgat gcttctgcgt ttatcgatcg tattaatgcc
     2041 acagccaaac aattaggcat gactaacact catttttcaa acgctagtgg tgcagcagca
     2101 caagcttttc aaggttatta taatcctact aaatatgatt tatctgcttc aaatattacg
     2161 accgctcgag atttgtccaa gttactctat gccttcttaa aaaaataccc tgaaattatc
     2221 tcctttacaa ataaatctgt tgtgcacact atggtaggaa cgccatatga agaagaattt
     2281 cacacctata accactctct cccagacaat caatttggta tgaaaggagt tgatggatta
     2341 aaaacaggtt ctagtcccag tgctgctttt aacgccatga taactgccaa aagaggcaaa
     2401 actcgactca ttactattgt catgggggtc ggagattggt cagatcaaaa cggagagttt
     2461 tatagacatc ctttcgttaa tgccttgact gagaaagggt ttaaagatag taagacctta
     2521 tccaaaaaag cacgccaaaa actcgaaaaa ttagtgccac aaaccaaaaa agaaacatca
     2581 cctaaacagc agcacttcaa agccactaaa aagcaatcgt acttggaaag agtcgaagat
     2641 tttatgaatc acaaccacac tttcttgctt atttgcttag ctatctttat aattactatc
     2701 cttttactaa gcctcgttgt ctttgcaatg ggacgccaat aatgaattat caaaaacctc
     2761 tgtcaaagca cagaggtttt tgatatgtta tcttacgtgt tcaaggcaac taaaaactaa
     2821 ccacttgggg ttagtactac aactcataaa atcctatctc aaagatcttt cagatggata
     2881 agagaaaatg caattaaccg acagaacctt ccatctcata ggaaatcaat cgattgagtt
     2941 cgaccgcata ttccattggc aattctttgg taaaaggctc cacaaatccc atgacaatca
     3001 tctctgtggc ttcgctttct gataagccac ggctcatgag atagtagagt tgttcttcag
     3061 aaatcttaga cactttagct tcatgttcta acgccacctg tgaattatga atttcgttaa
     3121 atgggatcgt atctgattta gaaatatcgt ccatcaaaat agtatcacat tcaatatggg
     3181 aaacggattt tttagattgc ttgttaaagg tcacttggcc acgataatct actttaccac
     3241 cagacttggc aattgatttt gacacaattg atgatgatgt atggggagca ttgtgaatca
     3301 ttttagcccc tgtatcttgg tgttggcctg cgttagcaaa ggcaatagac agcatggtgc
     3361 cacgcgcacc tggcccatca aggtaaacag aagggtactt catggtggtt ttagctccta
     3421 gattgccatc aatccattcc actgttgcat ccgtaagggc gcgtgcacgt tttgttacta
     3481 gattatacac attgtctgac cagttttgaa tggtggtata acgcatgtat gcaccgtcaa
     3541 gcgcaaagat ttcaacgata gcagcatgta agctgttact tgaataagtg ggagctgtac
     3601 atccttcaac ataatgaaca cttgctcctt catcaacaat aatcaatgtc cgctcaaatt
     3661 gaccagtatt ttcattgtta atgcggaaat aagtttgcaa agggatatct accttgaccc
     3721 ctttaggaac ataaataaag gtaccaccag accaaactgc tgaattgagg gctgctaatt
     3781 tgttgtctgt tggcggaacc agtttggcaa agtattgttt gaacaaatca ggatattctt
     3841 tgagggcgga atcggtatct gtaaagataa tccctagctt ttcaaattca cccttcatgt
     3901 tatggtaaac cacttctgac tcatactgag ctgatgcccc agcaagataa gcacgttctg
     3961 cttctggaat tccaatacga tcaaaggttt cttttatttt ttctggaaca tcatcccaag
     4021 aacgagctgg cttatcagat gctttttgat agtaaatgat atcatcaaag ttaatatctg
     4081 ataagtctgc tccccaggtt tgcatcggca ttttattaaa ggtttccaag gattttaaac
     4141 gaaactccaa catccactca ggttcatttt tggcagctga tagttcgcga accactgcct
     4201 cactcaatcc ttttcctgta gaataaatgg gctctacatc atcgtgaaat ccaaattggt
     4261 agtcccctaa gtcaattggc tttggctcta ctttctcatt tatatcagac atttttccct
     4321 tcctttacat tttggtccgt gaggtgctga gcattggcac ttcgtttaat ggcttccttg
     4381 agagcattcc aagctagggt agagcatttg atacgctgtg gaaattttgc aactcctgcc
     4441 aacaattctg cttcacctag ctctttttga gcaggatttt cctgtccttg taccatctct
     4501 gaaaaaatat cagctaacgc gagtgcttct tctttacttt taccaataac agcatctgtc
     4561 atcatgcttg atgaagctgt ggaaatggta cagccgttgc ctgcaaaagc aatatcttca
     4621 attttatctt cgtcaaactt aacggtcaaa gaaatcacat caccacaagt cggattattc
     4681 agttgaacag cctctacgcc atctagttgc ccatgatgat gtggacgttt cgaatggtcc
     4741 gctaccacag ccatgtatag atggttcagt ttagagagtg ccattgaaaa actcctttgc
     4801 ttttagaatt gcttcgacta gacggtcaca atctgccttg gtgttataga tataaaaact
     4861 tgctctaaca gttgctggta cacctaaata actaagaaga ggttgggcgc agtggtgccc
     4921 tgctcttact gcaacacctt catagtccaa ggctgttgcc aagtcatgag gatgcaaatc
     4981 atccagatta aaagaaatca gaccagatct tgcactaggc tggcttggtc catatatggt
     5041 aagcccctca atagcttcta atttcggcaa gacataggct attagttctg cttcatgcgc
     5101 atgtatatca gccatgccta gtctctgaag gtaagaaatg gctgcgctta gcccaatagc
     5161 accagctatg tgaggtgttc ctgcttcaaa cttccagggc aattctttcc aagtggcctc
     5221 ttgttcgtaa acaaaatcaa tcatttctcc gccaaattca agaggctcca cttgattcaa
     5281 aagctcttct ttgccgtaaa gaacacccaa acctgttggc cccaacatct tatgagctga
     5341 aaaagcaaag aaatcacaat ccaagtcttg aacatcaata gccaaatgtg gaaccgactg
     5401 ggcaccgtca acaacaaggt aggctccttt agcatgtgcc agcttggcaa tttctttaat
     5461 gggattgatg caaccaagaa cattggagac atgtactagg ctaacaaaac gtgtttttgt
     5521 cgtcaatttg tttgccaaat cgtccatgtc aagttggcca tcttttaaat aaacatagac
     5581 taaccttgct cctgtttttt gacaggcttg ttgccaagga atgatattgg catgatgctc
     5641 cataatcgaa atcaacacct catcttctgg cgtcaagacc tgctctgcaa accgagcaac
     5701 ccaattcaaa ctggttgttg ttcctctggt aaagagcact tccttacttg atttagcatg
     5761 aataaagtca gcaacctgct ggcgactggc ctcatatttg tgcgttgcac gttcagccaa
     5821 ggtatgaact cctcggtgga cattagcatt atcttcttga taataggatt gcaaagcctc
     5881 aagaacaagc gccggttttt gtgtggtggc ggcattatcc aaataaacaa ggggttcatc
     5941 attgacttgt tgatttaaga tttgaaagtc ttgtttgatg tcttttgcgt ctagcaatac
     6001 taacctcttt ctttttggca gtaggtctta acgattaagc aatttctcat ctaaaacctt
     6061 aataatctct tggtggactg atggaatagg aatttcagcg ataactgctc ctaggaatcc
     6121 tctaataacc aatcgttctg ctgtttcttg atccagtcct cgactcatca agtaatacat
     6181 atcttcaggg tcaacctgac cgatagaagc tgcatgacct gctgttactt cattttcatc
     6241 aattaagagg attggattgg catcggctct tgcttggtca gaaagcatca aaacacggct
     6301 ttcttgttga gcatcagctc ccttagcgcc ttttagaata tgaccaatcc cgttaaacgt
     6361 taaggtgcca cgttccaaaa tcacaccatg ctgtaaaata tgaccgaccg tacgttgacc
     6421 atagttggtc acgcgcgtgt caataccttg tacctgacga ccacttgagg ctgcaacaac
     6481 tttcaaatca gcttgtgagc cctgaccaat caaatcactg tcaaaatcag caatgacatt
     6541 gccttcattc atcacagcta aggcccaatc aatgttggca tccttctcta aacgtcctcg
     6601 acggctaata taggttgtca ctgaaggacc taagcggtcg atagccgaga atttaatctg
     6661 gctgcctgct tgggcaatca cttctacact gatattagcg ctggtctttt gagtggcatt
     6721 gccaatagat tcaaaacgct ctaaataggt gaacttactt tcttttcctg caatcactag
     6781 aacatgcttg ttaaaaggaa cgtcactgtc actatcttgt aagaaaatag cttcaatagg
     6841 agttgtgatt tccaagtgat caggaacgta gagcacggct gcgctattaa aataagcagt
     6901 gtggtaggca gctagtttgt cttcatcaaa agctaatgcc tgaccaaaat gagcttcaat
     6961 tacttctggg atttcctcaa gcgccgtata aaaatcactg aaaacaactc ccttgtcaat
     7021 taacgccatt ggtaactgtt ctaagactgt ttgcgtgcct acctgaacaa gctttgggtt
     7081 atctccaata gctataaaat ctggaacact agctagactt tcattttctg ttaaggtacc
     7141 atctcctaga ttccaacggt gaaatttaac cctttcgatg gttggtaact ctaattctgg
     7201 tatggcttct aatgccgcta aacgccgttc ttgcagccaa gcaggctcag cgtgggcttg
     7261 cgaaaaagct actagttttt cttttgtcat ctgtcaatcc tttgtctctg gtttttaaat
     7321 tggctgggta tatagaacat cacctagctt tttgtagaaa gctaaagtaa ttgtctccaa
     7381 aaacttaact ttcttcctta tactcgattc ccagatcctg tgcaatacct gcgtagcctt
     7441 ctttttcaag acgagtagcc aaggcagcat caccagataa gacaatacga ccatccatca
     7501 taacatgaac taggtctggg gtaatgtaat tcaacaaacg ttgatagtgg gtgataatca
     7561 tagctccaaa gtctttccca cgcatctcat taaccccttt agagactact tttagggcat
     7621 caatatcaag accagaatca atttcatcta acaaagcaaa tttaggctct aacatgagca
     7681 attgaagaat ttcattgcgt tttttctccc caccagaaaa accttcattg aggtaacgct
     7741 ctgccatctc ttctttcata cctaacaaag ccattttctc atcaagtttg gtgataaaat
     7801 ctcgcacgga aatcttatcc ttatctgcct taccggcatt cattgctgca cgcataaatt
     7861 cagcgttggt aatccctgga atttctgaag ggtattgcat ggcgagaaaa agtcccagtc
     7921 tcgcacgctc atcaacttct agatcgagaa tattgacccc gtcaagcaaa atttgccctt
     7981 gggtcacttc gtagttagga tttcccataa tagctgctga aagggtagac tttcctgtcc
     8041 catttggccc cataatggca gctacttccc cagttttaag agttaggtta acccctttca
     8101 aaatctcttt accttctatt gaaacatgta aattattaat ctcaagaatt gacatgatac
     8161 accccttatt ttctctgtca tctataccat ctcaagaaaa gacagtgtca tttgcactgc
     8221 tttttttata atggtcactg catctattat agcaaaaaaa gccttactcg gcttttttag
     8281 tgtttcaaat ccttcttttc tttccatttc agaagcattg cttgacgata atcactatta
     8341 ccaataaact ttaacaagtt aaacaaaggc gttcgctttt caccccagat ctccaatcct
     8401 tcaataaaaa cttctaaacc aaataaaagt ccaagcatca agaggacgcc accaattcga
     8461 ctagaaacat ttaaaagtaa agaaataagg gaaaaaagca tggtaatacc ataaactact
     8521 aagacagctc ctcgatgagt aaagcccatt gataagagtc gatgatgcag gtgcatctta
     8581 tccggctcgt agaatttttg acctgataaa ctacgtcgaa taatagccac gatggtatcc
     8641 ataatgggta caccaagaat aataacaggt gtcaccacag ccacagccgt tgaatttttc
     8701 aatccttgaa gagacaaaac ccctatcata aagccaataa agagggctcc tgtgtcaccc
     8761 aaataaatca tagctggatg gtaattataa ggaaaaaatc ctgcaatagc agaaatcaag
     8821 accaaaatgg ttaacgtcaa aaagaaatcc ttttgaggta aaaagaaata agatacaata
     8881 gccatggtca ctaaactaat aatagagacc ccactaacca aaccatccaa accgtcaatc
     8941 aagttaatag cattagtgat ggaaacaatc cacaggactg ttaagaataa ggttaagaaa
     9001 ggaccaaaaa ctaacaacgg ccctccaaaa ggaattttga agctatcaaa tttaaagtcg
     9061 gtaaaagccc agacaatcac tgcaccaatt agaataccaa acatctttaa tttaggactt
     9121 aactcataca aatcatctaa aaaaccagta agggtaatta ctgtagcacc aatcacgaca
     9181 ggtaaaatat aactgaagta ggtctgacca aaaaacatgg cgcctttaga agcaatcgga
     9241 ataagtccca aacttgctac taaaaaagac ataaaaatag ctaatccacc actggttggc
     9301 atcggaacct tattgacccg tctggcattg ggattgtcta ctgcacccac acgaaaggct
     9361 aaaaagcgaa caagaggggt taagaataag gacattaaca aagcccctat cagaacaagg
     9421 acataatcta ttgtgaatga aaacatacta ttttatccca actcaatctt ttgtaattgc
     9481 tcaacagcac catcaagcat caattgtaag ccatgctctt gtaaataagc tcttgttttt
     9541 gatccagggt ttgcatgctc aatcaatctc gcatacatga cattcgcaaa ataagatggt
     9601 tgttgctgaa catctaataa aatagtcata tggtagcagt tgctcccctt atataactct
     9661 gaagcttcta tcgaaaaatc aatcgtttta gcaaaagcca ctgcctctgt aatcgttgaa
     9721 aaatccaata cataatggac atagtctaaa ggttctgact catgattagt tgatggatca
     9781 gccgcctcag actgattagc tagagtagct tcaacaacat cttccattat ctcttcaatt
     9841 ttttccaatt tttcatgagc tttgacatct cccttttcac gcatggattg ctccaagctc
     9901 ttgaaaaaat cttccggagt catttgggac atatcaccaa actctgctaa atcttcaaga
     9961 ttaatatctt tatttatctc agatttggta acaaaaacat ctagacggtc cttgcgtgga
    10021 gtcactcgaa aactaagcat accactatct ttaaaattat ctggaagatc gagttcgtcc
    10081 atcacagaat agaaaaactc ttctgttttt tcttgaggaa tcaagaaatc tttcaactcc
    10141 attcctcttt cttctaaatc gtccatacta attgttattt ttagcgttgt ctcgctaatt
    10201 tgtttcattt ccattctcaa tacctcgcac gcgtagtcat tttattatac tagaaatgct
    10261 gtaatttcgc aatttatgac taactcctct tctccaaaag gaaatttaaa aagcgacagt
    10321 ccccaaggtc tgtcgcaaca tcctaaacga tatgtaagtt taaaaaacaa atttaaagaa
    10381 gctataaata agtaaaaggc ttcctaacac aatacggtat ttcccaaaaa tggtgaaatc
    10441 gtgtttctta acataatctg tcaagaacct aatcgccaat aaactaacca caaaagcggt
    10501 caaactagcc actaaaagaa taaggacttg tgcaaaatca aggtgatggc catcaaggaa
    10561 aaatttcaca gcctttaatc cactatatcc aaacatggta ggaatggcca aaaagaaggt
    10621 gaaatcagcg gccactgttc gactagttcc taaaataata gctcctaaga tggtagcccc
    10681 tgatcgacta gtaccaggca caatacttaa gacttgaaaa caaccaatga agaaagctgt
    10741 tttgtaagac attctagcta attcagtgac ggcaggttct tgttgagcat ttcgtttttc
    10801 aatccagata aaggcaatcc cgtagacgat taaagcgatg gcaatcggaa ccataaaata
    10861 aaaatgtgct tcaaaccaat tatcaagagg aaccgctatt aaaatagaag gaatacacgc
    10921 aatcaccact tttagccata attgccaggt aagctgtact tctcttgctg ttttcccagg
    10981 ttgaaaggga ttgagacgtt caaaataaat caacataaca gcgataatcg cccctaattg
    11041 aataacgata ttaaacattt ctataaaagc cttatcctga tttagtcgga taaattcctg
    11101 caccaaaatg aggtgaccag tgcttgaaac tggtaaccat tctgtgattc cctcaatgat
    11161 gccaaagaaa atggctttta gcaattcgat aattaacatg ttttctcctt attaaccact
    11221 cttattgtga ttttctaaac tgtgattaat gccaagcagc ttttttaaaa ggcaccgccg
    11281 ccgcctcctc cgccaccgcc agagaagccg ccaccaccac taatcccgcc tccggaagag
    11341 actgagaaat ttgaggaagt tgtagctgaa gacaagctag acaaaaaggt tggtgtcgta
    11401 gcatacatta ccattgataa ttcacccggt cgaacagctt ggtaaacctc aggtaaggca
    11461 atacgatgga ctttcaaata gcgttccact ttcttagcgt aaccaaataa ggtggcataa
    11521 accaaaacac ggttccaaac aaccaaacct tcaatagcaa catcctcaaa tttgtcaatg
    11581 tctcgtatca tgtttttaaa gctctgccat tgatgtaacc gaacaccccc ctcaggtgtt
    11641 tcaataccca atttcttgta ttgatctaac ttcaggctaa tgtaaaagcc aaggcacatc
    11701 gttagcacca ttaaagcaag gtaaatgagg gctaacccac tttctttaat agccaagtaa
    11761 atcaataaac cactatttag aacaagcaac aaacaaccta atccctggac actattgctt
    11821 tttcgtttct ctgccggggt catttgacga taagtatctg gtaagtttaa ctttttgata
    11881 gtttctagga cattgtttgt aatagctgcc gacgcttttt tcatcgcctt aatcacctgc
    11941 tcagacgatt gacggacttc ttgctcgagc tttttacctt tgtacgtttt ctttaattgt
    12001 ttaatggtat cagcatcata atggtattga ctaaacaatt gatccaccgg caatgtcacc
    12061 ttgtttccaa aagccatatc taagaaggca gcttcctcat ctgtcacacg atctaactga
    12121 ctgatttcaa gataagcttt cccttcttct ttcgttaata aaagcacctt ttggtcgatc
    12181 agatccaaca aggtagcttg aacaagggcc tcaaaggtca ctcctttagg gatcaataat
    12241 ttctggcttt ctgatgctgt aggggataaa taggcaaaac tctgtccata aatcgcttgt
    12301 gtcaacacaa gaggcgaaag atcttcaggg acttcgtaac tgtgatctgt atggttggcc
    12361 aaatgataac gattgaattg tttctttaat cttataaact gtattaactg ccacagaaga
    12421 aaacctacct ctaccagagg aatgactttg ccaaatagca gctgtaacaa ggcccttcgt
    12481 ctggaaatct tagtttccaa cgctacaatt ttattctttt tactcgttga aacatcaact
    12541 ggttcttctc ccaataatgc ttttttatcc caataagcat gcaattccaa ttgccctgag
    12601 acatttttgg cattaatctg atagcgactg ttaccttctt ttaacacttg aggtttcttt
    12661 tggtaatatc ctctgtgagc ccatagatta gaatcctgaa tatcagttgg ggtagtgacg
    12721 gtcaaactca ccttttctaa tgttttgtcc caatcactaa tcggtgtcca attcagctct
    12781 gccacgtctt gataagctgt taaaaggtgg tgcagttgcc agataacctt aacgtcaacc
    12841 ttatcaccag cttggccagc attataaagt tttaaacgat agccatctcc aaggtcacta
    12901 aactcttgac taacaggtac ctgttgacca ttctgatata cctcaacttt aggcttttgg
    12961 tcaatagcaa agcccgctgg caaatgacct gcccggccca gcgaaatata ctggccgtta
    13021 taagaagtgt caaattgata ggttactttt tgctcaaacc gagcagtatt ttcttttgat
    13081 aacagtaact gaccttcata gttggtgatg ctatagtcta catcagctgc ttttattcgt
    13141 atccctaaaa gactgaagca gagcacaagc gtcatcaaaa tttttttcat ggcaacatcc
    13201 tctttctgcc ctcattttac catattttaa ggagatgaca aaacctaaac caagtccttc
    13261 atcttatttt taatcattat tttgaatatt tttccatatt tcaacctatt cactgataat
    13321 atattgaaat aggatgcaaa ttgctgtaaa ataggtctga atactatttt tgaaacagga
    13381 gacctatatg aaaaagctaa tccttagctg tttggtcgcc ttggcccttc tgtttggagg
    13441 aatgagccgg gctcaagcaa accaatattt aagagtcgga atggaagcag cctatgctcc
    13501 ttttaactgg actcaagatg acgcttcaaa cggggctgtc cctattgagg gaactagcca
    13561 atacgccaac ggttacgatg tccaagtcgc taaaaaagtc gctaaagcta tgaacaaaga
    13621 actgttagtc gttaagacct cttggaccgg tttaattcca gcattaactt ctggaaaaat
    13681 cgatatgatc gctgctggta tgagtcctac caaagagcgt agaaacgaaa ttagcttctc
    13741 aaacagctac tacactagcc aacctgttct agtcgtaact gctaatggca aatatgctga
    13801 tgcaacaagc ctcaaggatt tttctggagc taaagtaact gcccagcaag gcgtttggca
    13861 tgtcaatctc ttaactcaac taaaaggtgc taagttacaa acaccaatgg gagatttctc
    13921 tcaaatgcga caagccctta cttcgggtgt tatcgatgcc tatatttctg aacggcctga
    13981 agccatgact gctgaagctg ctgatagccg tttgaaaatg atcactctta aaaaagggtt
    14041 tgctgttgct gaatcagatg ctgctatcgc tgtcggaatg aaaaaaaatg acgatcgtat
    14101 ggcaactgtc aaccaagtgc ttgaaggatt ttctcaaacg gatcgtatgg ccctgatgga
    14161 tgatatggtt accaaacaac ccgtggaaaa gaaagccgaa gatgctaaag catcatttct
    14221 aggccaaatg tgggctattt taaaaggtaa ctggaagcaa ttcttacgtg gaactggaat
    14281 gacccttctg atttccatgg tcggaaccat tacaggtctc tttattggat tattaatcgg
    14341 tattttccgt acagctccta aagctaagca taaagtagct gccttgggac aaaaactctt
    14401 tggttggtta ctcactattt acatcgaaat cttccgtggg acacctatga ttgttcaatc
    14461 tatggttatt tactacggaa cagcgcaagc ctttggtatt tcgatcgacc gtaccctagc
    14521 ggctattttt atcgtatcta tcaatacggg tgcctatatg agtgaaattg ttcgcggtgg
    14581 tattttcgct gtcgacaaag gtcaattcga agcagcaact gctcttggct ttactcacgg
    14641 acaaaccatg cgtaaaatcg tgctaccaca agttgttcgc aacattttac cagcaacagg
    14701 taatgagttt gtcatcaata tcaaagatac ttctgttttg aatgttatct ctgttgtgga
    14761 actttacttc tcaggtaata ccgtagccac acaaacctac caatatttcc aaacctttac
    14821 gattatcgcc attatctact ttgttcttac cttcacggtg acacgtattc ttcgtcatat
    14881 tgaacgccgt ttcgatgccg atacttacac cactggagca aaccaaatgc agattgcgga
    14941 ggtctcaaat gtctaactca attattgaaa tcaaaaacct caaaaaatct tacggcagca
    15001 atgaagtact caaagatatc tccctttctg ttaacaaggg agaagtgatt tccattatcg
    15061 gttcttctgg ttctggaaaa tcaaccctcc tgcgctcgat caatcttttg gaagagccaa
    15121 gtgccggtca aatcctcttt catggtgaag atgtccttgc tgaacactac aacttgactc
    15181 actaccgtga aaaactaggc atggttttcc aatcctttaa cttgtttgaa aacctaaatg
    15241 tattggaaaa tgctatcgtc gctcaaacca ccgtcctcaa aagagaccgt gcacaagccg
    15301 aacaaatcgc taaagaaaac ctcaatgcgg ttggtatgac agaacaatac tggcaagcca
    15361 aaccaaaaca attatcaggg gggcaaaaac aacgggttgc cattgccaga gccttatctg
    15421 tcaatccaga agccatgctc tttgatgaac caacatcagc tcttgatcct gaaatggttg
    15481 gtgaggtcct taaaaccatg caagacttgg caaaatcagg gttgaccatg attattgtta
    15541 cccatgaaat ggaatttgct cgtgatgttt ctgaccatat catcttcatg gataagggat
    15601 tgattactga agaaggaagt cctcaacaaa ttttcgagaa tcctacacaa gatcgtacca
    15661 aagaattttt acaacgcttc ttaaaataag ataaaggatc caactatttc tatgccagtt
    15721 gggtcttttt tcaattcaaa aagaatttgt cataaaatac tttaccagtt aactatttta
    15781 tgctatactt atttttgtta acgttaataa tttatattta ggaggataat atggcagaaa
    15841 aaactgactt atcgtcagct tacagaagac tgaaaagtcc caatattaaa accagaaaac
    15901 gtgctctgaa gattattcac gagtacaaac gttacggtaa aaaataagat tttacttttt
    15961 atctctccaa atttaagtca gacaaaaaaa ggctaagaca ccccttccat cttagccttt
    16021 ttgtatacag tttattaagg gaactatttt aaaatatctt ttatgctaga gtctagaata
    16081 gttcaaaacg ttaaaatcat acatttaagg tagtgtttaa agtatacgaa aaagaactca
    16141 gcataaacca agctctcttt cgttataact aattatttag caatttttgc gaagtactca
    16201 agagtacgta caagttgagc agtgtaagac atttcgttgt cataccatga tacaacttta
    16261 accaattgtg atccgtcaac ttccattact ttagtttgag ttgcgtcaaa caatgaaccg
    16321 tatgatacgc ctacgatatc tgaagaaacg attggatctt cagtgtaacc gaagctatcg
    16381 tttgaagcag ctttcatagc agcgttgatt tcgtcaacag aaacgttttt gtcaagagtt
    16441 acaaccaact cagttactga tccagttgga acaggaacac gttgtgcagc accgtcaagt
    16501 ttaccgttaa gttctgggat aacaagaccg atagctttag cagcaccagt tgagttagga
    16561 acgatatttg cagcaccagc gcgtgcacga cgaaggtcac caccacggtg tggtccgtca
    16621 aggatcattt ggtcaccagt gtaagcgtgg attgtagtca taagaccttt ttgaataccg
    16681 aatgcatcgt gaagagcttt agccatagga gctaaacagt ttgtagtaca tgaagcacct
    16741 gagataactg tttcagtacc gtcaagaatg tcgtggttag tgttgaaaac aactgtttta
    16801 acatcgtttc caccaggagc tgtgataaca acttttttag caccgttagc atgtaagtgt
    16861 ttttcagctg cttctttttt agcaaagaaa ccagttgctt caagaacgat ttcaacccca
    16921 tcagttgccc agtcgatgtt ttctggatca cgttcagcag aaactttgat gaagtttccg
    16981 tttacttcaa atccaccttc tttaacttca actgttccat caaaacgacc ttgagttgta
    17041 tcgtatttca acaagtgtgc aagcatattt ggatctgtaa ggtcattgat acgagttact
    17101 tcaacacctt cgatgttttg aatacggcgg aatgcaagac gtccgatacg accgaaacca
    17161 ttaataccaa ctttaactac cattagtgat ttcctcctta tgaaaatcaa aagaatatta
    17221 tttagaggga atgctcccta cctattgtga aaagattaac ttacagtcat ctgcaacaac
    17281 ctttcaacag aactattata taactatttc agaaaaaatg caaataaatt agccattttc
    17341 ataaagggca gcttctttct tctagccttc aaaacccacc aaatatagga aagggctttc
    17401 ttttttctat gttctattct gtaatttatc tttttattgg gaaagatttc caaaacttca
    17461 aactgtacta taacgttctg ttacactaaa aaagaggtta ggatagttat cctcaacctc
    17521 ttagtggtgg ttattcacct ttgtttttct taatgatttc ttcttgaact gacttaggaa
    17581 catcttcata gtggtcaaat accatcatga atgtaccacg tccttgagta gcagaacgaa
    17641 gaactgttgc ataaccgaac atttcagcaa gtggaacata agcacgaacg atttggctgt
    17701 taccatgtgc ttccataccg tcaacgcgtc cacgacgagc tgtcacgtgt cccataacgt
    17761 ctccaagatt gtcttctgga gctgtaatcg ttacaagcat catcggctca aggatagctg
    17821 gttgtgcaga tttagctgct tctttaagtg caagtgatgc agcgatcttg aaggcagttt
    17881 cagatgagtc gacatcgtgg tatgaaccat cgtaaagctt agctttaaca tcaaccattg
    17941 ggtaacctgc aagaacaccg tttgccatag attcgatcaa ccctttttca acagctggga
    18001 taaattcacg aggaactaca ccaccaacga tagcattttc gaattcgaaa cctttacctt
    18061 cttcatttgg tgtaaattca atccaaacat caccgaattg acctttacca ccagattggc
    18121 gtttgaagaa tccacgagct tgtgtagaag cacggaatgt ttcacggtaa gatacttgag
    18181 gagcaccaac gttagcttca actttgaact cacgtttcat acggtcaaca aggacatcaa
    18241 ggtgaagttc acccatacca gcgataactg tttcaccagt ttcaacgttt gtttcaacgc
    18301 ggaatgttgg atcttcttca gcaagttttt gaagggcaac acccatcttg tcttggtctg
    18361 ctttagattt tggctcaacc atcaactgga taactggttc tggaacttcg attgactcaa
    18421 gaataacttt tgctttttca tcagtcaatg agtcaccagt tgttgtgtct ttcaaaccaa
    18481 cagcggcagc gatgtcacca gcgtaaactg tttcaatttc ttgacggctg tttgcgtgca
    18541 tttgaaggat acgtccgata cgctcacgtt taccttttga tgtgttcata acgtaagaac
    18601 cgctattaag aacacctgag taaacacgga agaatgtcaa acgacctacg aatgggtcag
    18661 tcatgatctt gaaggcaaga gctgcaaatg gctcttcatc agatgctgga cgttcttctt
    18721 cagcatctgt atccgggtta acacctttga ttgcagggat gtcaagtggg cttggaaggt
    18781 atgcgataac cgcatcaagc atcaattgaa cacctttgtt tttgaaggca gaaccacaaa
    18841 gtactgggaa gaattcaacg ttgattgttg ctttacggat accagcaatc aattcgtcgt
    18901 tagtgatttc ttcaccttca aggtatttca tcatcaagtc ttcatcagtt tcagcaactg
    18961 cttcgattaa tttttcacgg tattcttgag cttgttctaa gtattcttct ggaatatctt
    19021 cttcaaggat atctgtacca aggtcgttgg tatagatttc tgctttcatc ttgatcaagt
    19081 caatgatacc acggaaatca tcttctgcac cgattggcaa ttggattggg tgtgcatttg
    19141 cttgaagacg gtcatgaagg gtttgtactg agtaaaggaa gtcagcaccg attttatcca
    19201 ttttgttggc gaaaacgata cgtggaacac cgtattcagt tgcttgacgc caaactgttt
    19261 cagtttgagg ctcaacacct gattgtgagt caagaacggt tacagcacca tcaagaacac
    19321 ggagagaacg ttgtacttcg atggtgaagt ccacgtgtcc tggtgtgtcg atgatgttaa
    19381 cgcggtgacc atcccattga gctgttgtag cggcagaagt aatagtgata ccacgttctt
    19441 gctcttgctc catccagtcc atttgtgatg cgccttcgtg tgtttcaccg attttatgaa
    19501 ttttaccagt gtagtaaagg atacgctctg ttgtagttgt tttaccagca tcgacgtgag
    19561 ccatgatacc gatattacga gtttttgcaa gtgaaaattc gcgagccatg tgtttctcct
    19621 atttttttag tttactcttt cattatagca ctatttagca aaaacggata ggcaggacct
    19681 acccgttatc taaatatttg ttggtaaaga taatacctat tgcctttata aggcttggta
    19741 tcttattcat tgaactcagg ctaaacgctg tgtgaaaaag ataaactttc ctagagacaa
    19801 aggtttcttc gtcaaatttc ctattttcac tttgcgttct atacgccttc gtatcttatt
    19861 aattgagttc gagctacaag ccactgacaa aaagataata gttcctagaa actgaagttt
    19921 ctgcgtcaat ttccctattt tgtctagcat ttgtttgacg ctaaaatatc ttatattacc
    19981 aacggaagtg agcaaaggca cggttagctt cagccatttt gtgagtgtct tcacgtttct
    20041 taactgatgc acctgtgttg tttgcagcat ccatgatttc tttagcaaga cgatctttca
    20101 tagtgtgttc accacgagca cgtgatgcgt ttaccaacca acgaagtcca agtgttgtac
    20161 gacgttctgg acgaacttca actgggactt ggtagttaga accaccgaca cggcgtgcgc
    20221 gtacttcaag tacaggcatg atgttgtcca tagctgtttc aaatacttca agagcgtcat
    20281 ttcctgttgc ttctttgata gcgctgaaag catcgtaaac gatagtagca gctgtaccac
    20341 gtttaccgtc aagcataaca cggttgataa gacgtgttac gatttttgag ttatataatg
    20401 gatctggtaa tacttcgcgt ttaggcgctt gatttttacg actcattttc tcttatcccc
    20461 cttctattat ccttttggac gtttcgcacc gtatttagaa cggccttgtt tacggtcagc
    20521 aacacctgca gtatcaagtg cgccacgaac gatatggtaa cgtacccctg gaaggtcttt
    20581 tacacgtccg ccacggataa gaacaacgct gtgctcttgc aagttgtgtc cgatacctgg
    20641 gatgtaggca gttacttcga taaggttgct caaacgtaca cgagcgaatt tacgaagggc
    20701 tgagttaggt tttttaggtg tcattgttcc aacacgagta gcaacgccac gtttttgtgg
    20761 ggcagccatt ttagtttgaa cttttttgtg actgttgtaa ccaatgttca aagctggtga
    20821 atctgatttt tcaattttag atttacgtgg tttacgtacc aactggttaa ttgtaggcat
    20881 ctacattctc ctgtgttttt ttatttttgg ttgatatagc acttggtgac agcccatatc
    20941 cggtgcactt tttgcaacaa ttgtcagcac gtctctgtac acttttgaga gaccaaaagt
    21001 aaaaagtacc gtctaatata ataacacaga cggtacttca tgtcaaatag aatttttcct
    21061 ttgatgcaat tgcttatgac gaatgcatca cttagattcc ttacggaacc taaatcctgt
    21121 gaggccgaca gctgccaaca tgacgactcc tgctttaaga gctctttgag tactttcatc
    21181 aacatcagaa atgagattgc tcgttgtaga agatcctgag ccataagatg tcttagatga
    21241 gggctgagta atcgaagatg tgattttaga cacaatggca ttacttgcta ccatttcaga
    21301 ggtttggcca acaagcgctt ctgctacaag acttgtattt tcagcagcta atcttgctga
    21361 agcttctaat agttgtttcg tttctttaac catttcttga acaagcggag tagtatctat
    21421 cttactatat gatagcggtt ttacgcccgt aagaggtgga gctacttgca aatcaggcac
    21481 agtagctata tcttcgtcta aatggcgata tcccttttca ttagctaagg ttttaagcaa
    21541 agtcaactgg tgttctgtgg tagcaatagt agcttctaga ctgctttgtt tagcttgtaa
    21601 ggctgctaaa gtttcttgtg catttaacaa agatgaggta gttttagcta aatcttgctt
    21661 agtattatca atgcgctcac gtatcacttg aaggcggtta ggattcaatt taaagtccct
    21721 tagatattgc aaatgagctt ttttagccac cagtgctgtc actctggctg cggcttgctc
    21781 tgctaagact tctgtctggt gcagtgcggc tttcaacgat gctagcttag ctaatgattg
    21841 atcacgagta gcttcgagtt gtgcttgttt tgctttagct gctagtaatt ctgttctcaa
    21901 agaaccttta gtatcattta attgtctcac ttggaggttt aagctgtctg actgcttaag
    21961 tgtgcttgct aatttacctt gaagttgact tactttagct tgggctgcca taatgtcagc
    22021 ttcttgatga atagccgaca aacgattttc taatgagctt acttttcctt tgatcgctgc
    22081 aatagtatca gatacagtgc ctactctttg ggcataatct tttgtacttc caacggcttt
    22141 tataggggtc ttattaaagc gttgatggtt agcaatgtta gactctggaa acattacaaa
    22201 gtgttcattc aaagatccta cattgctggt tgaaaatcca aggtaaacag gcgcattagg
    22261 gttacgttta tctacacgta aaaagttaat agcatggccg tatgtatttc cgtgtaaatg
    22321 atctgtaaag agcatatact tgatactgtc ataaatacca cgtttaatac cattcacagt
    22381 atgcacatcg ttaaaagcac cgatattctc gtacatgtta tcatcatttc gaatgagccc
    22441 tgacgctccg gcagagtctt caataatagt tttatcatga ggcccaacac cataatgccc
    22501 tgatacccct ggctgtccgt agacaaatga tggtcttgta ttaccatgag ttttcttata
    22561 gctggtacta agtaatcttg caaattcttg tgatcctgct gtaacagtaa ctggtggtag
    22621 acctaattgt cttcttacac tattaatcat gtgagctgca aactgcgcta gctcattttg
    22681 cacttctggt gtcaaattat cgggatcaac aaagcgatta cgatctgctg gaatatcttg
    22741 gtattgattt aattgattac ctggactagc tttggcaata atttgatctg catgctcttt
    22801 gtaataatta ttgtaactag ctgatccaat ataaccacta gcttctaatt ttttaagttc
    22861 ttcaagagga tagccttgcg gtactttcat ggtattatta cccacaatgc tatcttgagt
    22921 agacggagct gaggatttaa gacgactaag ttctgcctct ttttctgcta gagcagcttt
    22981 ctgagctgcc aactcttcag tcaattgctt tttaacttta gctttttgat tttctaagtc
    23041 agctttagcc ttctccaatt ctgagcttaa tgcttttgta ttatcattag ccgtttgagc
    23101 tgctttagtg atagcatcag gattgctaat catagcattg agcttagcaa tattttgttc
    23161 agacgttttg acttgttcca ctaaatcttg agctcgagta gtttctgctg aaatgctagc
    23221 tttttgttct gacaatgcag tctcttttga atgttgatct gcttgagcat tatgaagctc
    23281 tgtttcagta gctgttaact ctctttgatg ttcggctcct tgggctaata gcgtctcctc
    23341 actacttgca agagtattag tgtaaatttc ttgtgcagag gttaaagctt tttgttcatt
    23401 atcttgctgc tcttttaagt ggttgatttc agcagtagtt tttgtcagag cggtagcaag
    23461 ctctgtcagt tctgcttttt gttgactgag agttttttca actgcatcaa tagttgcctt
    23521 tgcctcttga attgtttctg gttttggtaa actatcgtcg tgagtattac tcgccttcgt
    23581 ttctcctgag gctctatcat ctgctttgac ttggtgagat acgcctacac tggcactcaa
    23641 taaagcaatt gttgaggtta aagcaatttt ctgcttaact tggtttggct tcgtttgttc
    23701 taagtccatt ctctctcctt tttccattta tcgaaaatat taccaacttc tacaacaaca
    23761 ttgttgtttt tgacatcaat ctctgaaact ttcagcaatg atttgaaggt catttgctca
    23821 cgctctgatt gagcgttttc tgcaaacaca gcaacaccaa ctaaagtgct gtcaaattct
    23881 gtcaagagac tgatcattcc tgtaatcgtc cccccgccct tcaagaaatc atctacaatc
    23941 aatacacgac tattgggttt caaactacgc tttgacagaa acatcttttc aatacgatca
    24001 ctagaagcac tcgcataatt gacagacact gtcgatcctt ctgttatttt taaatcacgt
    24061 ctaacaatga caaaaggaac acttaaaata ttagccactg catttgctaa aggaactcct
    24121 ttggttgcaa ctgtcatcac agcgtcaatc ttttctcctt taaaggcatt ggcaataata
    24181 cgaccaatat tttgtaaaat tttaggtgta ctcagcaagt ctgacaaata gatatagcct
    24241 ccaggtaaga tgcgatcact ctctgataac ctttgacaca ggtcttcaac aatggtacgc
    24301 gcttctgttt ctgaaatact gggggtaaaa attacgcctc cacttgctcc tgtaagtgta
    24361 tcgatatcac caatgttagc ttcttcaaaa gctttcttga taatagcgat atcttctgag
    24421 atagaagatt ttgcggcctc atatttagtt gcaaaggtgt ttaaactagt taatttatac
    24481 ggattgttaa tcaaataatt tgaaatgaca accatacgtt cacttcgtct taatttcata
    24541 gtttccccat ttttatgtac tttttgctat tataccataa agttaataaa aaacgaacat
    24601 tcgattagga aaatattggt ttttttgttc ttattaatat catgcaagaa ttcaatctaa
    24661 gataaactaa tcaacattcc tactgaaagg acattctact taataaccat aacaagagcc
    24721 aaaaacactt atgaaaccat cataagtgtt aagcacatca aaaatcatca caaaagcaaa
    24781 gtaactatta cttgagaaac actgatcaat agtttggctt gtaaaaagac cgattatcca
    24841 tggcaaaaat ccgattggtc atctcgccct cgctaacacg gctaagagct gttgtcatca
    24901 tcatcatatt agcatcaata ttgtcaatca tatgaataat ttcagcttcc atgatgcgtg
    24961 gacgtaccgg actaccgtat tctaattgcc cgtgatggct taaaatcacg tgcctaagga
    25021 caatgacttc ttctttggta tcatcgattt gtagttctga aattactttt gtaatttctt
    25081 cgttaatgag agaaatatgc ccaatcaagt taccacgaac ggtgtattct gtattatctg
    25141 gtcctgttaa ttctatgact ttagctaagt catgtaacat aatacctgca aacagtagac
    25201 tcttatttaa gtcagggtag atatctccaa tgctatctgc tagtctgacc atcgtagcag
    25261 tatggtaggc gaggcctgac tcaaaagcat ggtggtttgt tttagctgca ggataagtat
    25321 aaaattcttt atcatactta cgataaagag cccttacaat tcgttgccac gttgcatttt
    25381 caattttaaa gagcatctgt tccaaatagt cacgtacttc tgtcacactg acaggtgcct
    25441 tttctttgaa gtcctttgga tcattgggct cacctgggcg aacatttcgc aaggtgattt
    25501 gattaacctg aggggtacca ttatacacct cgcgtcgccc tttcataaac acgactttac
    25561 cagcagtaaa ttcttcaacg ttataaggtt gggcatccca cagatttcct gaaatctcgc
    25621 cagtgtcatc ctgaaaggta aggctaataa aatctttacc agctcttgtc ttacgaacct
    25681 ctgccgactt aatgaggtag aacccttcaa aaagctggtc ttttttcatt tgattaattt
    25741 tcattatttt cctcttctaa taagggcatg ttcaagagag attttgttgt ttggtcttga
    25801 taagtttcaa cggtattcaa ggctcgaaca atggcatttg tccttgttga aatgagctga
    25861 tccagcgtat tattagctgt attcatttgc ttttgagcct tggcaagcag gccaccaaat
    25921 ttatcgaatt ctaacttgac attgcccaaa attttactga tgtcatcagc atttttttgg
    25981 atattaaggg tcttgaaacc aacagataag gaattgagca aagcagacag ggtcgaagga
    26041 cctgcaacca caatattttc ttcccgacga aggctatcaa agaaagccgc atttctgacc
    26101 acttctgaat aaagaccttc tgttggtaag aacataactc cgaaattggt cgtctctggg
    26161 gggttcaagt actttttatg aatgtctttg gcaaagcgtt tgatagctgc cagaagtgct
    26221 tttcggctag cctcgatggc cagtttatca ccaacttcgt aagcatcttc taatcggtaa
    26281 taatcttcaa gagggaattt tgagtcaatc ggtaggtaaa tataaccgcc ttgaccattt
    26341 cctgggagct taatcgcata ttctacgcgt tcactagaac cactaaccgt tacaaattct
    26401 ctttcgtact ggcttgatgt catgatatcc tcaatgattt ggcctagttg aagttcgcct
    26461 aaaatgcctc gtgttttggt attggacaaa accttattta aagtacccac atcttgagcc
    26521 acgctacgca tttctcccaa gcctttattg acactttcta gttgcttgga tacagaatcg
    26581 aaagaggcgt gcagacgatt ttttaaggtt tcttccaatt tttcttcaac gatctggcgc
    26641 atttcctcca aacgtttttc gttagattct tgcatatttt tgagcgattg gttgacctgc
    26701 tggttaattt cttctaagcg tttgtcagac cggtccctac tatcagacaa actacggtgc
    26761 aagacgtcac gaatatctgt taattgttgg taaaggtctg cttgttgtcg gttcatcagc
    26821 tgtgttagct ctaacaattg ttgcttgtta gctgtatcca actggtaggt tatttggtca
    26881 gacaaattat ccgcgttgcc ttctagggtt tgggcaagct gatgttgaag gccgttgact
    26941 ttgaacaaca gataagcccc taaacctaag agaaccaaga ccaaaaggaa caagataagg
    27001 tccatactaa ctccaatctt tactttgaat aataatcaag tagccacttg gcaaactaac
    27061 tgtaataggt tgatggataa aggcattact ggaatagatt ttctttttga aaaaattatc
    27121 ttgcgtcaac tcaaatttag caccagtaat ggtcaagtca gcttctcctt ctgccataaa
    27181 tgccacatag gtcatccctt cttcttgatg gataaggtgc tgacctgccg ggcggtaggt
    27241 aatcatattt tgttggtcac gcaaggcgat ttgtgccata aagggagcaa taccagggtc
    27301 gctaggcaag aaaatattgg ataagaggtg gtccattcga cccccaaaag ccccaaaaat
    27361 ggtcacttcc gcctcaggaa aacgagcaaa gacctcttta agggctaatt ccgtatccgt
    27421 atcatttttt tctggatgag cagtaataaa aagctctgcc ttctctttta tatccgtaaa
    27481 cgctttttgg gaaaccgaat caaaatcacc aacagccata ttcaaaggaa gcccattttc
    27541 taataaaaat aggctccctc tatcaatacc gacaaaataa tcaaaatcac ggctgatata
    27601 agagagatct cctcccgcaa aaagagcaac tttagacatt caaggccgtc cttaaggttt
    27661 ggacttggct cactaggtca ctggccttga aaaggtaaga acctgccaca aagacattgg
    27721 cccctgcttc ataacaagca cgaatggtct tattgtctac tccgccatca acctcaatgt
    27781 caaaggacaa tcctttttca tctcgccatt tggctacggt agccactttt tctaaacatt
    27841 ctgggataaa ggcctgaccc ccaaaacctg ggttgactgt cataatgagg acttggtcaa
    27901 ctaagtctag caaaggttct agggctgttg caggagttcc tggattaatc actacccctg
    27961 ctttcatgcc agctgccttg attttttgaa gggctccatg aatatgacga gtactttctg
    28021 tgtgaatggt catgatatca acaccagctt gagcaaaagc ctcaacatag cgttcaggat
    28081 caaccaccat tagatggcaa tcaaagacca atttactgtg ctttctcata cttgccacaa
    28141 catctgcacc aaaactgatg ttaggaacaa attgaccatc cataatatca atgtgaacgt
    28201 attcggcatc tgtttcttca atacgagcta gctcagaagc aaaattagca taatcagctg
    28261 ctagaataga tggggcaatt tttagagttg acatggagta gacctacttt cttttgataa
    28321 cttttttata tgtttcacga cggttttcaa tttcactgag aaattgcagg taatgctcat
    28381 aacgaactgg ccacaactct cccgtttcaa gcgctgcctt gacagcacac ttgggttcgt
    28441 gggtatgggt acaggatcta aacttacact catggctcag ccggcgcagc tctggaaaag
    28501 cctcgttcaa atcttcagca ttagcaatat catagtctaa tgatgaaaaa cctggcgtat
    28561 ccgcgatttt tccgccatgg gtattgtaaa aactcacagc tcgggtggtg tgacgaccac
    28621 gccctagact atctgagatt tctcctgttt caagagccaa ttcgggggcg attcggttga
    28681 gaagggttga tttcccaaca cctgtttgtc ccataaagac ggtaatttta tcagctagca
    28741 aaggcaacaa ttcctcaaga ctggtcacaa aatcgtaacc gattgcctga tactgtcgac
    28801 caatagcctt aatctcctcg ggactatcta ataagtccat cttagagata taaaccactg
    28861 gatgaatggc tttgtgttca agcaagatga gaaagcggtc tagaagattg ctgttaaatt
    28921 ctggttcttt agcagacata ataaccactg cttggtcaat attgacgata ggaggtctta
    28981 ccaaactgtt tttacgagga tgaatcgcta aaatataacc ttcagagttg tcctctgctg
    29041 agaaatccac gatatctcca acataaggtg tctcaccgcg cttcctaaaa ttaccacgcg
    29101 ctcgcgtctg atatacctgt ccttctgact ccacgtaata aaaaccagcc agagatttaa
    29161 tgattttacc ttgcaactgt gctcctttgc cctactataa taactctatt atatcagaaa
    29221 accgctccca aatcagcatt tgactctaag tttcactcgc cttaaaaaac tgtcaagatt
    29281 ctctagacca ctggctatca tttaggtcac tgctttttaa aaatagaata ttttatcaac
    29341 taaaaatcag atcactgatg atttttgagg taggaaacga tatgaatttt aagaagacct
    29401 ttgctattta cgaaaaagag tctaaccgta ccgctatagt cacgctcttg ttaattgccc
    29461 cttttttccg gggtattaaa aaaactagct ctgcactgcg ataccttaac ggccagcttc
    29521 aataagaaaa atccccctga actgaacatt tctgttttgt ctacaaggga atttttttat
    29581 tgtcaccggg agaaagcttt tataatccaa cttcttttaa agcatcagct aatttgccaa
    29641 aatcttgaat cgacaaggcc tcaccacgaa tagaaggctt gatatctgct agtgctagac
    29701 ctttttcaag tttagtcttg atgtcttcag acttcccaaa atggctagtc aagttattcc
    29761 acagggtctt gcgacgatgg acaaaactga gacgagatac tctgaaaaag aaatcttcgt
    29821 ccttaacctc aatcaaaggc tggtcgcgac gcaccatttt gaggatagca ctatcgacat
    29881 ttggtgcagg tacaaagacg gtgcgcggca caatgaaggc aaccttggca gtcatgtaat
    29941 actgcaccgc aatggacaag gaaccataag ctttagtgtt tggctcagcc gagatacgat
    30001 ctgccacttc acgctgcatc atgactacaa actcttggaa tggaattttg ctctcaatca
    30061 agtgcatgag aataggcgtt gtgatgtagt aaggtagatt agccaccacc ttaatgggca
    30121 aatcaggatt tttaaactgc ttgatttggg tctgcaaatc agcttttaaa atatcttgat
    30181 tgaccacttg cacattatca aaatcgcgca aggtatctgc caaaatcggc accaagcggt
    30241 catcaatctc aaaagccatc acttcagcag cattttctgc caaaaattcc gtcaaagccc
    30301 caattccagg accaatctcg ataacattga cattctgatc aatctcagcc gtgtcaacga
    30361 ttttctgtaa aatattggta tcggtcaaaa agttctgacc aaaagatttc ttaaaggtaa
    30421 atccgtgacg atcaaggaca gccttggtca cgctataatc tgcaattctc atatattctc
    30481 ttctttttat gatcactttc tttttggcat ttgaaccact agcctaaact tttttgccaa
    30541 gatacgccat tcagttaatg gggctgttat attttattgt actctttact cctcatatgt
    30601 ctccatcact tcttccactt ctgctagggt aatgccaaat aattcgaggc gcttgagcag
    30661 ttgtttgccg ttggcatagc cgatgcggag cttttcgccc aagtattccc tgcgtttacg
    30721 gctatctgac gccattaaaa gccctagacg aatcaggtct gtttgacgaa tgtcaaaata
    30781 agactcatca tcatattgct gagtgacatg agcaagtgcc ttttggaggt cctcaaaatt
    30841 ggcatgttca acccctaagg aacggccctt actcttagaa ctcggaaccg cctcgtttcg
    30901 gtttaaaaag gcgtgacgag ctgttggcac agctgccata atgagcttac gaatgcgctc
    30961 accattataa tctgggtctg tcaaaacaat gacaccacgc aagtcattta ggcggttaat
    31021 ccgttctaaa tcttcttcag tgatagcaga gcctctggtc tcataggtat ccacctcgta
    31081 aaaacggcga aggttagccg tgtcgtcctt gccttctaca acaagaacct cttgaatatt
    31141 aattttttca gtcaagctta agtaccctct ttgcattagc agtcgtttct tttgcaactt
    31201 cttccacggt cattccccgt agctctgcga ttttatcaac cacataacgg gtataagctg
    31261 tatgattttg tttgccacgt tttggaactg gtgtaagata aggggcatct gtttccacca
    31321 aaatcttatc caaaggcaag tgctgcgctg cctcttgaat atcaagggcc tttttgaaag
    31381 tgaccacacc tgaaaaagaa atcatcatgc caagttcaat gaaacgttca gccatctcta
    31441 atgatcctga gtaagaatgc atgatgcctc cacgagggcc aacaccagct gctttgatga
    31501 cctcataggt atcttccaag gcatcgcggg tgtgcaccac aaaaggcaaa tcatggtctt
    31561 ttgctaattg catttggcgc ttaaaaacct ctatttgcac ctctttgggg tcctccatcc
    31621 aataatagtc taagccaatt tcacctaagg caatgacttt aggatgtgat aattgagaga
    31681 cgatagactc ctcaactgct tcagaataag atcctgcttc tgtcgggtgc catccaatag
    31741 tagcatacac gttaggatac tggtctgcga gagcaagtgc tccactaata gtggcctggt
    31801 caaagccaac cacattatgg taggccacgc ccatttcttg agccaaagtc aattcttcag
    31861 tttcgtgtcc ttgaaattct gctacattca ggtgcgtatg ggtatcaaaa atagttagtt
    31921 tttctacttt atttgtcatg atgttattat aacataaaga tcttaaccac atcagaaaac
    31981 ttggtgagaa cttctgccgt ttaggttgat cgtaaaaccc tttgctagct gacacttgtc
    32041 tattttctac ttttttgaca aatgaccttt aatagtcggc tcctacataa gccctcaaaa
    32101 atactacaac ctactcaaat aaagtgggtt gaacaaatct gtatcacaaa aagggtcgct
    32161 accttctgtt agcgatctat gctatactag tagatagtta aactgtaagg agtatgcttt
    32221 gctatgccta aaaaccaccg cctaattacc aaaatcgaag catcattgga acatatgacc
    32281 agtttagaaa aagggattgc tcattttttt attacaaccg atctgacacc tcaagagttg
    32341 accgcatccg agatagttaa gcgactacac atctctcaag ccgccttgac tcgatttgca
    32401 aaaaagtgtg gttttactgg ttatcgtgct tttgcctttg attaccttca cagtcttcaa
    32461 gaatcacaag agactttcca atctattcac ttagaattga caaagcgcgt gctgatggac
    32521 tacgatgctc ttattaataa aacctatgag ctcgttaatg aagaaaaatt actgaactta
    32581 gccaagctga tcgatgcttc tgagcgagtc tatttctttg gcaaaggatc ttctggacta
    32641 gtagcaagag aaatgaagct acgctttatg cgacttggtt taatctgtga tgcttattca
    32701 gacactgatg gttttacttg ggcaaatagc ctcgtcaatg aaaattgcct tgtttttggt
    32761 ttttctttat ctggcaaaac aaactctgtc atcacagcac ttcatcaagc tagtcagcga
    32821 ggggccaaaa ctgtcctctt aacaacagat aatcaaaccg aatttgacga ctccttagat
    32881 attattcccg tatcatcaac ccaccaacta cattatggga atcgtgtgtc accacaattt
    32941 cctcttctta ttatgatgga cattatttat gcttacgttt tagcgattga caaaccgcac
    33001 aaagaaaaaa tcttcaaaaa cactattatt gacaaagaaa ataacggcct ttagactaca
    33061 atagcaaaaa gggcgttgtt ttctttttaa agggtcgtca ttcacctccc ccagtatact
    33121 taaacaagaa ctcttcacgt catatagact cacagccact aagattagct actcgctaaa
    33181 ttattttcga ttttctcaag agttataaat gaacaggatc gctcattctt ttgtttaaaa
    33241 tggtagtaag ctcctaatat accagcatta tttccatgac tagcaaactt cagctgagtt
    33301 ttcttggcaa gactagaaac caagtagcta tcaagagctg tctttaattt atcagctaaa
    33361 tagtcttttt gcaccatgat tccgccacca agtaccacca catttgggtt aacaacgtag
    33421 cagatattag caataccttg tcctaagtaa tccacctgct tactaatggc tgctatacaa
    33481 atcgcatcac ccgctttagc ctgctcaaaa atacgacgac catcccattg gctaatgtca
    33541 tccccataag ctaaggccac ctcttgaaca agagctgtgg tagatgccag atcctgaaac
    33601 tgaccatctg acaaatgaag gtaaccaact tcgcaagctg aatgactact accatgaaaa
    33661 acttgggaat taaacaataa acagccaccg attcccgtcc caattgtcaa gcaaagagcc
    33721 acaggataat ccttggcact gccagaaata gcttcggcta gaccagcaca gttaacatcg
    33781 ttttccactt cacacggtaa cccgaaagtc tcttcaatct cttttttaaa ctgagttcca
    33841 gcatagttag gaatctgagg gccggcataa aagatttctc ccttatcagg attaaccatt
    33901 cctgcagaag aaatggccac tccagccaga tccatttggt cttgataggt ctttaccaag
    33961 cccttgacct tttccaaaat agagggacca cctttataag cctcagtggc catttcctct
    34021 ttttccaaca ggtctcctgt ttcagaaatc aagccatact tgatagcagt accaccaata
    34081 tcaattgcta aataatgctt catttgttat cccctcgcat cttattaaaa ggtttcctta
    34141 gcctgattaa tcagagcagc agcccgttgg caaataaccc tatcttcctc gaccaactca
    34201 gctaaaggtg atcgaacaga accaatatca agaccctcat taatacgaag tacttcctta
    34261 ataaccccat acatattgcc gtgagcagaa acaaggacac cgatagtctc attaatagta
    34321 tattgaaggg tcttggcctt ttctaaatcc ttgtcaacta taagttgatt cagccttaaa
    34381 aataattctg gcatagcccc gtaggtgcca ccaatgcctg cagcagcacc catcaagcgc
    34441 cctcctagaa attgttcatc tgggccatta aaaacaatat gatcgtctcc gccaatcgca
    34501 cagaaggttt gaatatcctg cactggcatg gatgaatttt taacgccaat cacgcgctta
    34561 ttagccaaca tagtcttata aaggcttggg gtcaaggcaa cccctgccag ttgaggaata
    34621 ttatagatga taaaatctgt gtgaggagca gccgaagaaa tcgcattcca ataatcagca
    34681 actgcatatt ctggtaaccg gaaataaata ggcggaatag ctgcaatggc atccacgccc
    34741 aaacgttctg aatgagctgc caattcaata ctatctttag tgttgttgca ggcaacgtga
    34801 ttaataatcg tcagcttgcc cttagcgact gccatgacat tttctaacac caattgacgg
    34861 tcaaaaacgc tctggtaaat acactctcca gaagagccat taatgtaaag cccctgaacc
    34921 cctttgtcaa tatagtactg ggtcaaggca cgcacacgct cagggctaat agctccttgg
    34981 tcatcgtaac aagcataaaa ggcaggaata atgccttggt atttcgtaag atctgtcata
    35041 gtaatgtcct ttattcaatg tcattattaa gtgggaatcg cctaagaagg gattccatga
    35101 taatcaagta gaggtaagtt aggctactga tgcccatagt ggccagtaga gacaagccac
    35161 aaaataaggt taacagggtt aacggcaagg taaaaaccaa aaggccaatc accactagaa
    35221 aagcaaacgt ccatgggaat aaaattcctg ccagcagtaa acttcgctta aagagtatta
    35281 aaagaggaag ctcttttctg acggcctgag gataagcgta caaaaagagt ataactacca
    35341 aaaataaaac agcaatgcaa agtactttaa acccttgaaa aactaggcct gattggtttc
    35401 gaattaagaa aagatctaag acacaaatac tcattatcaa aagctcaata agacctaggc
    35461 gcatcccact tttaaaataa tgtttaaaat gagagctata agtagtaaca agctgactaa
    35521 cttgaccttc tcgccaatcc aacaaactcg catacaaagc cattttagca accccaatcg
    35581 ttagaagggg aaggcaggac aagacaaata agagattaaa cacgagcaaa tcaaacagtg
    35641 ctgcgctagc cctcatccat ttactatcaa gcttgaagag agagtgtaaa agtccctgtt
    35701 ttttcgatgt caaatcacaa cttcctttct agaaatacgc ttacaaacaa aagctaattc
    35761 ccctcttaaa cgtttaacac atgacttgct tcccatctga tttatctatg tatgcaccta
    35821 aattaataag caacctataa attaagctta gcgacacgct ttatttagtc taacctttca
    35881 cagcacccat agtaatgcct tgggtaaatg atttttggaa gacaagaaat accgtcacaa
    35941 taggcactgc agccatggca gcccctgcca taatcaagcc ataattagta gccatttcag
    36001 cttgcatagt ggcaacccca agtgaaatcg ttaaattttc ccgagaggtt aacatcacca
    36061 actgcataaa ataatcattc caagtattga taaaggtaaa aattgctagc gctgcaaagc
    36121 ctggtttgac aatagggaaa gccacattga aaaaggtacg aatttcgcca catccatcaa
    36181 tcttggcgga ttccaataac tcggttggaa tattttctga gaactgtttc ataaggaaaa
    36241 caccaaatgg ccagcccaca agaggcaaaa taacagccgc caaagtgtca tggattccca
    36301 taaaattaac aatccgcaca agaggcacta gaacaacctg ttttggcaag gccatagcag
    36361 caataaaaat cgaaaacaaa agccgctgtc cataaaaccg ctttttggcc aaagcatagc
    36421 cagccaaaga ggaagtgcca caaaccaaga acatggtcgc caccgaaata aagacactat
    36481 tccacaacca ttttaaggct ggattttgca ctaccaaggc cttaaaattt tcaatagtag
    36541 gtgcctttgg ccaccattgc ggtggaatca taatggtatc ggcttgagcc ttaaatgctc
    36601 ctgtcataat ccaataaaat gggaaaataa acaaaatggt caagatgcat agaataacag
    36661 tagtcagaac atccgatgcg gttagtttct ttttcgtcat cttaatatcc tcctaatatt
    36721 ccacatcatt acctaaaatt ttaaattgag caaagctgat gattgcaatc atgactgcca
    36781 agaacacacc cattgtgttg gcatagccgt attcagagag cttaaaggct ttttcataca
    36841 aatagtacat gagggtacta gttgaataat ttggccctcc agacgtcaag agttgaatca
    36901 aagcaaaaca ttggaaggaa ttaatggttg ttatgacagc aatatataag gttgttggca
    36961 ggagacttgg ccacttgata ttccaaaaca cctgaaattc tgtggcgccg tctacacgcg
    37021 cagcttccat caaagaatta tcaatatttc ccatagcagc aatatataga ataataggct
    37081 gcccaacaga tgtggttaat aaaatagcaa taatagctaa caaagcccag tgcttatctc
    37141 ccagccaact aatgttttgt tcaatcacat gagcatattt taagacataa ttcaaaatcc
    37201 ctgacatcgg atcataaatc catttccaaa ccaccgttac ggcaacactt ccggtaacaa
    37261 caggtagaaa gaaaactgcc cgataaaacg aacgcgccac cacatttttg tcataagtct
    37321 tagccgccac aaagagggaa aagaaaacta caacaggtac cgaaccaata acgataatca
    37381 acgtattgat aagagatttc ataaagatag gatcttgaaa catcctagca taattggcaa
    37441 aaccaacaaa ggtaaaatcc gtcatggaat aattgaaaaa actcgtcaca aagcccataa
    37501 tcatcggtat caagacaaaa atcacaaaga aaaccaagac cggagctaag aaagcgtatg
    37561 agatgagcgt ctctcgcatt tttaatttat tgacgttcac ttgaaatacc tcttttttct
    37621 gttcaacttt tctaaaaaga aaagcttctt tccagtgacc atttgacatg tgcattgaaa
    37681 agaagccact tgttctgaac acttattgtg tttttttgat tgtcttgttc gctttttcag
    37741 tgaaggcttt caaagcatct tctggctttt catcaccatt agagacggct tgaaccattg
    37801 ggaaccaaag agttctcatt tcagcaaacc catcaatcgt gttgtagtat ggtgagtaga
    37861 attttgtcca ttcagcaatt ttttccattc gtttgtcttt gtaaagatcc ccataagaag
    37921 ttcttacagg gaaagcacct gtacgaacaa catttttagg tccccattct ttatcatcag
    37981 cgataaactg gataaatgtc ttagaggcag cgactttttg ttcatctttg ttattaaaga
    38041 ccgcaaaacc atttacgagg tattctagtt ctggtttgcc atcatctgat gggaatggga
    38101 tttcaaggta atccacttta ctagcttcta acaatttagc ttggatgccg ggttgtgcag
    38161 gcgcccaaag aatggtaaag gaagtttggc cattcgcaaa gttttgaata tcagctgaac
    38221 cgtcgtattg agagccattc atcattaaac catctttaat ccaattggaa attttagtca
    38281 tggctttaat agagttagca tcatctgtgg tgtatttggt tactttgtca tcggtaatat
    38341 gactactata aaggttagca aagaaggcac gtggtccttg gtcaccacct tgaccattag
    38401 caaagaaaga acctgggtta tatcctttgt ccttcaatgc tttcaagact ttttcaaaat
    38461 catctgttgt ccaaccctct ttcacaaggt caaggacacc agcatctttg agcatttttt
    38521 tattcaacgc catgtagaat ggagcagagc tgataggata catgtaagct gtatctcctg
    38581 cttttgaggc ttgaatcaat ttgtcattgt taacatcttt ggtaaactct tctgtgaaca
    38641 agtcattcaa gtcagccaat ttcccatttt taccatattg aatgatacga cctggagcat
    38701 caaagaggac atcaggagct gttccagctt caatagcagt tgtgattttc tcaggaccag
    38761 atgtaaaatc aatggtttcc aattttacct tgatttctgg attagctttc tcaaaggcag
    38821 caatcaattt cttctcatag gttcccacac catcttcagc tttttcttgc gtgaagaccg
    38881 ggaaagccca ccatgtgatt tctgtcttag cagtatcaga tttacttgca ccagcctctt
    38941 tctggctctt tccgccacaa gctgctagtc ctaaaacaga tgcaccaagc atcactaatg
    39001 aagctaactt cttcatgttc atcgtaaaat ctcctttttt tgatgtgtgt ttctattttg
    39061 acaaccacta ttaggatttg agcgcttcga taaaacgctc tgcaatttcc ttaggccttg
    39121 taatggcccc tccaacaaca atacctgcca cacctaagtc attaattttc ttggcttctt
    39181 ctggggaatg aatctttcct tcggcaatga cagcaatgcc tgctttgcaa agagcttcta
    39241 tcaatgccac atcaggacct gcttcctgac ggctatacgg ggtgtagcct gacaaggtgg
    39301 ttccaacaaa atcaatgcca gcctgatggg ccactagtcc ttcatcaaag gtactaatat
    39361 ctgccatcaa aagctgattg gggtactttt ccttaacttg tcgaataaag ctagcaatat
    39421 ccaagccatc atgtcggtca cgcttggtac aatccatagc aatcactgca atatttaagg
    39481 cagctaattg atccacttcg gccatcgttg ccgtaataaa aggctcttgc ggcgggtaat
    39541 cttttttgat gataccgata attggtaggt ctgttatggc ttgaatttct ttaatgtctc
    39601 taaccgaatt agctctgatg ccgacagcac cagcttcttg agcagctttt gccagcagtg
    39661 gcataatacc cccagtctct gaatagagag gctcacctgg caaagcctga caagaaacga
    39721 taatcccacc ctttaactgc tccatgagct tttctttggt tggtttatca ggcatagttt
    39781 ctactccttg tgtttcttag ttttaataaa aataacgttc acaaagttat tataacaaaa
    39841 aataaagcgc tttcaatagc aatgagactt ttagaatcta ctttcatttt ttatattatt
    39901 ttctattatt tggaatgctc tttcatacaa caaataaaaa ggtaaggagt tgacaccata
    39961 cttatacatt gcaaattccc gattagaaaa cgttaaaaaa ccaagagcct cgagcactct
    40021 tggtttactt tagttgtatt ttttgcaatt aagctgataa tactttacgt cctttacgac
    40081 gacgtgcagc aagcacgcga cgtccgtttt tagttgacat acggtgacgg aatccgtgtt
    40141 tacgttgacg acggatttta cttggttgat aagtacgttt cacagtgaat acctcctcat
    40201 agattttgta ttcgtttagc cggctatttg tgatatcagt tactaaacat actataatat
    40261 tctatcttaa agagggtcta ctgtcaagat ttattttcgt ttttcaacag tttctctctc
    40321 atttaagagg ccgctcattt cataaagtgt ctcttctttg acactgttcc tttgccttac
    40381 cgttgaaacc ttatcaatag caacaataat caatgagatc agtaaaacaa acggttaagc
    40441 tggccataca aaaaatcttc gatagctaaa ctaactacca aagattcttt ttaggatcta
    40501 acgttgaaga ctgactacca catagcggtt agggtcattg ccttcagaat aactatcaac
    40561 cccttcaatg gatgaaacgg ttttatgaac gattttacgc tcactgttac tcatgggatc
    40621 catggtgtaa tcttgaccag attccaagac gcgtttggca actttttgcg taaagtcaat
    40681 caaggtttct gttcgatgtt ccacataatc atggacattc aaggatactg aaaaattttt
    40741 tgaatagcgg tcgtgcaaga aattttgagc taacaactgc aaggatttca aaaccttacc
    40801 atggtaacca atcacacgtc ccgcctctgg cgtttcaatc tgcaaattga tttggcgacg
    40861 attgttgctg gtttcaacgg tggcttcgat atccatttca tagatgattt tagtcacata
    40921 atcggacact tcctgagcag ctttttcaat atctttgctg aaaccatcat taccaggatc
    40981 ttggggcact tctgattcct cgactgggat tgacggttgt tgtgcttttg tttcagtgac
    41041 tgtctcagca agattagaaa gggtttgcgc tggactatct tcagacatga gaggaaccac
    41101 tttggtatct tctgcttcta acttttgagt ggcttttatt tcttcaggtt ctacatcagc
    41161 agaattgacc gcgggagcat ttttttgatt aatatcctct ggaacacctc gagtggcttt
    41221 tttatcagcc ttataaaccg tcttgtcgct gataccttcg atatcaactt gggctggctt
    41281 tttaccgaac ccgaggaaac cttttttctc ttttgaaatg actttgatat gcgctttaag
    41341 gcgtgataac cctaactctt gaagtcctgt ttcgatagct tcttcgactg tttttcctgt
    41401 aaataatacc ataacctaaa ctcctatttt ctcttcattg cttttttacg agcacgacgt
    41461 tcacgaagac ggcgttcttt ttcttcattg gcaagacgct gtctttcggc aataatctta
    41521 aaaggattgt ttaatagcag tagttgtacc acttgaaagg cgtttgagac cgtccaatag
    41581 agcactactc cactagccaa gttaaagccc atgaaaaaga tcattagcgg catcacataa
    41641 atcataacag tcatcatgac atttttttct ttagccgcta gattggtcaa ccaagtggac
    41701 aagaaagtga agacagctgc caaaactggt aaaaggtaca aatgatcatg ttgtgccaat
    41761 tccacccaca agaaagtccc tgtttttaag aaagataccc gtgtcaaggc ttggaatagg
    41821 gcaatcataa ctggcatctg aattaagagg ggtaagaggc tagcataagg gttaacccca
    41881 tattttttgt aaagggcttg actttcttca gccaacttca tccgcgtttg tgtgtcttta
    41941 ccagcgtatt tcttttgcaa ttctctaagc tcaggttgga tgtcttgcat cttctggctt
    42001 gatttgattt gcatattaaa caaaggcatg agcatgagac ggatagtaag ggtaaaaaga
    42061 ataatgccaa caccaattga accatcaaag gaaagccatt gaattgctct ggcaaataag
    42121 taaaccaact ggtcccaacc actagatgat tgtgccgtta cctcaccacg accacaggcc
    42181 actaataata gaactaaggg gactattctt gcaattttaa tgtttttctt cactctcaaa
    42241 acctttctca agcaattgtg ctaactttaa aacatgatgt aaattttgtt gaagctcttg
    42301 atactccaaa gactcgacac ccttacgggc aatcaccacg aaatcctctg acttcagctg
    42361 atgccctaat gccatgataa catgacgtat ctttcgtttg actgcatttc tggtgactgc
    42421 atttcctatt tttttaccga cagaaatacc cacacggaag tggtcttggc ctctatttaa
    42481 atgataaatg acaaattttc gatttgctgt actttttcca tccttaaata tggcttggaa
    42541 atctttctca cgcttgacac gataggtctt cttcaaaatt taactccaat atctaaatta
    42601 ttaccattat accacatccc ctaaaaaatt aaaaagccct aaccctttca gattaagcct
    42661 ctttctgtac tatttctagt ttttgtgctt caactgcctc ttgtcactcc atcttttcgt
    42721 agtatagttc ttctttcgac aaaggaataa ccttcagaaa acaaccataa aaagtcagaa
    42781 tccacaagac gtcagctact tatccctgta tttacctatc aaaaaacccg gcaagccggg
    42841 ctttgatact gattttacaa tcagctaatg tgatatccaa acaaagacaa ctgacatcgt
    42901 cattttgtct gccgagccat catatcaaaa atactatttg tttatggaga gagagaaaac
    42961 aatattttga ttgaattggg gattgattgc tcgataaact catgacttcg taaatcttta
    43021 tttacactaa ttattatagc gaaaaatgtt tcaataagag catcatttcc tgaaatgttg
    43081 ctttttcgtc ctaaaatgtt attgctctct taactttagc gaagagcctc ccaccgttca
    43141 aacagacatt tcatttttaa tcgtgatacc atacaagaat caccattttc gaaataaagg
    43201 agctgcttac tcttatcaat tcgaacaacc ttatctagat tcacaaggta ggatctatgg
    43261 cacaaaaaaa gtttcggtgc tactgcttga atctcagata gattaccata aaactctaat
    43321 ctttctgtct gagtccataa acaaactttg tgaggagttg tggctgtagc aaagtataaa
    43381 atatccttat agggcaattt taatcttgtc tgtggagtct caaataaaaa catgtccttt
    43441 gactcacgac tagacatcat atcgtaagta tatcggatac attcttcgat ttgttcttta
    43501 aactgttttt gaccacctgc tttatcaata aaatctagag cggatacctt atatttaaaa
    43561 gaaataggag caaattctga atgagtggtg acaaacacaa taacggcatt aggatctttt
    43621 tgtcgaatag ctgcagctaa ttcaagccca caacgagtat attcaccaat ttcaatatct
    43681 aaaaaataca gctgatggtc tcctctttct tggatactct caaataattt ttgaggagtc
    43741 gagaacactt caagctgatt acatggtatt ctagtctctt ttaagatgcc tactacgata
    43801 gattctatgc gtgtctgctg gataaaatca tcctcaagaa taaaaatgtt catcatactt
    43861 tatctcattt ctaacaattg tcttaatcga taatggtcac ttttagtcga aagcgttatt
    43921 tgggggtaac tatctaaaac agtcaaaaag tgggctgagt tctgctgctg agcatcaaag
    43981 cgtttggcta tatccacacg attggttttt gtgctatttt caataataaa taattgcttg
    44041 ttatcttgtt taaagtatgc aatagataaa aaaggacgtc tactgccttt ggctgtctca
    44101 atagcattgt ccactagtcc agtaaaaagt acaattaagt cgattaactt aataggtaaa
    44161 tgatcgataa catctggaat ctcaatataa agttcaatac ccaaatactg agcttcaagc
    44221 aatttggaat tcatgatact gcgaatcaca gcctctttga tattgtctaa agaagatatt
    44281 tcataatttg tgcgcatcat agaagttgcc gattgctgaa taaccgtgtc ataaactcta
    44341 gtaatcagat ctatatcacc actatcgata ctatctttta gactaattaa gatattttca
    44401 gaatcatgtt taactgttct gatttctcgg taaagttgct caatcgattg attgtaattt
    44461 tccagatacg caatacgttc attttgtgct tgtgttaatt tttgtgacag ctgatcttta
    44521 gcaaatcgat ctaattttac aataacccaa ataaaaatag ctaaataaat aaatactaaa
    44581 atagaccggt actggaaata tagtgcaaga aatccgcttt gcacagttac caaaaaatgg
    44641 gcgaaaaagt agtaagaaaa catggcagta ttcatccaaa atacccactt tttcatctta
    44701 tcttcactga taaaccttat taagcttaag tcaatactga aaatgtagct aaacagataa
    44761 aatattggta atacaaaaag ataacataac tctaaaagct taaggttatt attaatttga
    44821 ccaatgctaa atcctaagac agctggtaaa aagaaagatc cgatgacgcg aaacagtaat
    44881 tcgacaatca agatagtaaa gaaaccatta aacatatgct caaataattt ctttttcgga
    44941 gcacttaaca aactaacaat aataaaaaac agttggtcaa tcaaaatgac tttgtcaaat
    45001 atcatattag caattacaaa actaatgcct ataacgctaa cccgtttcca gcttagttta
    45061 atagcagaaa cctttgcaaa aatgacccag ctgttaataa agatggtagc atagaataaa
    45121 gccatactgt atataaccat catctatcca cctcctcctt tggcatggtc aaggtttggc
    45181 taaattcctt atcaaaactt ttagtccgca aggataaata aggatacttt ttgagaatac
    45241 gctgcgcatt agaaaggccg ataccacgtt ctgaaccttt tgaagaatag ccttcttcaa
    45301 agatcttgtt aatgtctacc ttttttttag tcgtatttgt cacaacaaac atctgatagt
    45361 catctaacaa aaagtaagca attgacatat gaggttgctg tgcttcaaga gctgcctcaa
    45421 tggcgttatc acaaaaaata gacatcaaca atagtaagtc caactcagga atgtaagtct
    45481 cttttatatt atctggcact tccacattca aatcaatacc tgatttttcc gcactgataa
    45541 ttttagctga caataagctc ttaatcgatg agatattaat tttcctaagc ttcgagatat
    45601 tatagtgttt atcatcccag tagtcgttag cttcatgaac cgtttgggca taaatatctt
    45661 gaatctgagt aatggattca ctcttaatgg cttgacctaa acgctctata cggctcaggt
    45721 aatcactttg aaaagctctt acatctcgat agagtttctc aagatgtttc ccataagtct
    45781 ctaagttagt catataagct tgttgttctc ttctcaactc atttttaagg tattctttag
    45841 aactatgatt aaaataagat agaagataaa ctaaaagaag tataaacatc aaaaacaata
    45901 acttataagg tgtgggaaca aaaagagaat gatgaaaatg taaaaagggg tccgtaaatc
    45961 ctaaaatgac aaaaatgtcc acataataaa ccaaaacaaa aatcagcaat gtttgataaa
    46021 aattatgcga tcgtttcgaa tatccggatt ggcatatcat cttgtaatct tttccaatca
    46081 tataattaat aatagcaaaa cttggaaaaa tcagcagata agcaagcaag tcaaagataa
    46141 tatgacccag ataaatacca ctcgacaaaa aaagataggg gataacgatt gtacccataa
    46201 accgagatag caaatccacc aaaacactag gtgcaacagc caaaaaaaga gctttcaaag
    46261 aaaataattg cttggtttct tgccttaaaa caactaaaaa taatagagga tcaattagca
    46321 atggcgacaa atacgggaac tgattgaaaa atagggctaa agccaaaatc gttagttgtt
    46381 tccaaactgg taatgtgata tcagatagaa aggaaaaaat ataaattttt gtcatataat
    46441 agacaataat tgataataat aaaaacatat attcgctatt agccattttt ttctcctttc
    46501 caactttatt attttagcat attttatgac aatgattaca atattatttg attacaattg
    46561 attagctaag tgaatactaa actagtctct ttcagcatat accaaaaaaa tattgtacac
    46621 taattgattc ataaaaacca tttctttaaa tcacactaat caaaacctca ccgtttgctg
    46681 tcattatcac gtaagcatct caccacctct ctttcaaatc caatctccaa aatagaaata
    46741 aactatacta ttttttactt taccaaggta gcaagctcag ataacgataa aaaagactga
    46801 agaacttaat cttcaatctt tcgttgtcgt gactttttaa ctccttaaag cgtttaaaaa
    46861 taaaggtgaa cgatacagcc ttatagcatg tttttgatgt gctcaatgga tttatcacgt
    46921 cccaataaat aaatcgtatt tggtaattct gggccatgca tttcaccaga tactgcaata
    46981 cgaataggca taaagaggtt tttccctttg attcctgttt ctttttgaac agccttaatt
    47041 tgtgggaaaa tattttctgg tttgaagtct tcatctgaca tagcttccaa cttagccttg
    47101 aaagcttgca agactgtcga aactgtttca cctgccatca cttctttttc ggcttctgtt
    47161 aattcaggga aatcagaaaa gaaaaggtca gtcaatggaa taatctcatc tgcagatttt
    47221 aattgaggtt tgtataattc tactagcttc tcagcttttt ctgtcaaacg gcctgcctct
    47281 tcaaggaaag gtttgcagag ggcataaact gtttcaaaat ccgcatgttt aagatactca
    47341 ttgctcatcc aatccatttt cttctggtca aaggcagctg gcgacttgct aaggcgattt
    47401 tcatcaaaaa gggcaatcag ttgctcacga gagaagattt cctcttctcc gccaggattc
    47461 cagccaagaa gggcgatgaa gttaaagaca gcttcaggca tataaccttt tttgcggtaa
    47521 tcttcaatga attggagggt attggtgtcg cgttttgata attttttacc cgtttcagaa
    47581 ttgatgataa gagtcatatg accaaactct ggcgcttccc agccaagagc ttcatagacc
    47641 atcaattgtt ttggcgtatt ggcaatatgg tcatcgccac ggataacgtg agaaatttgc
    47701 atatcatgat catcaacaac aacggcaaag ttataagttg ggtagccatc tttcttttga
    47761 ataacccagt caccaccgat gttaccacct tcaaactcga tatcaccttt aaccatgtca
    47821 gtccatttgt aaataccgga ctcattaaca gcaagacgga ctgttggaac gatacctgct
    47881 gcttcacgct cagcaatgta cttagctttt tcgtcagctg acataccaat aaattcattg
    47941 atatagcgag gcgtttcgcc agctgcttct tggcgttcac gctctgctgc caattcttcc
    48001 tcagtcacgt aagacttata ggcttttcct tcagctaaca attgatcaat atattgttga
    48061 taaagtgcta gacgttcaga ctggcggtaa ttttcatgcg tttctgggct ttcatcccaa
    48121 tccattccta accattttag gttttcaagt tgagaacgtt caccatcttc aacatgacgt
    48181 ttacggtcag tatcttcaat acgaatgata aaagtacctc cgtgacgacg ggcataaagg
    48241 taattaaata gggctgtgcg ggcattcccg atatgtaaaa gccctgttgg acttggtgca
    48301 taacgcacac gaattggttt agacatgctt cttctcctgt tacatatatc actcagaaac
    48361 ctgtggcacg aaaaacaagc aacaaatgat gatttgagct tgccaagacc ttttcagact
    48421 tcttctaaaa tctttcttat tatagcatat tttacttgct taggcgaaaa aaaatcagcc
    48481 ctaaaaagct gattttatgt tattttgact aatttgaact agttggtaga taacgtttaa
    48541 gagtcccagc aaattgctca aggttaagac tttggatata aatctcacca tgacctctaa
    48601 aggtattaac aactccttca cctgtaccaa ttgactgcat aaagccattt tctaaatgaa
    48661 tatcataatc aagttctctg ctccatgcta caacatgggc attatcaata gtcattgtgc
    48721 caccatcaag tgtgattttc ttgatagatc caaacgagtt agcaagtaag gtaccaaggc
    48781 cttcagttgt catgacaaaa agtccgcctt gaccaccaaa aagtgctttt ccaatatttt
    48841 gacgttccat tttgtactgt gctgaaccat ctagggctag aaaggcacca tcatttagac
    48901 gatattgttt ttcacctagt tctaaagcaa caatctgtcc tggtgtattg ggtgctaatg
    48961 ctaatttacc atctccattt gacatagctt gtgtaatgaa cattgattca ccagaaacca
    49021 tggaacggcc gatggctcca actaattttc ctaaaccaga tcccttacca tttaatttgg
    49081 tattcaaggt tacattctca gtatgataaa ccatacttcc ttgttgtaag taaactgatc
    49141 ccccatgttc cagatcaatc tcaactagtg gaaattgcat gttttgatca atcgtaaatc
    49201 tcattctatt gtcgaccatc ttactctcct tgtttgtatg aataacttga tacaattatt
    49261 ataacgcttc cctatttctt tgtctaatat tttcaataat ttttaaaaat aaaaaagagc
    49321 tgaattcagc tctacaactt ggctactaac tattccatta cttgtgtcat ctttccagta
    49381 gccacatcgt agacggcacc gttaataacg acatcatcag ggataagaga ggaggctctt
    49441 agcttagcca tatccttgcg gacactgtct tccacatctt ggaagggaag gaaatcttgc
    49501 cctgaaacat ccacacctaa gtgctcatga atatgcttgg caaagccttc attagtaaaa
    49561 gtctgcgcgc cacaatctgt atggtggaga accacaatct ctcgcgttcc catttgctgc
    49621 tgagaaataa ccagcgaccg aatcatgtct tcggtgacac gtccgccagc attccttaaa
    49681 atatgagcat ctcctagagc cagccccaga gcttgggcca catgtagccg tgaatccata
    49741 caagtcacaa tcgctacttt agtcttgggt tttaagggca gatgagctgt cccatgcaag
    49801 gccacataag cctgattggc agccataaaa tgttcaaaat aagacatcaa taaactcctt
    49861 ttgagaaaca gcagagctat ttttgctgtt agtttgtctt atcttaacat cttttatgga
    49921 aaatagctgg attaacctct tagacggtcg gtatcgttcg aaaagttaaa aaaacaaatg
    49981 attgggatac tttcttgaca aatttcctcc tcatttgctc ttagtttctg atcctctagc
    50041 taaaaactgc ctttaaaacc tgaccaaccg tagtcactcc aacaacctca ataccttgag
    50101 ggatatcaat gccttgcaag gcatttttag gagcatagac cttggtaaag ccaagctttg
    50161 ctgcttcatt aatccgttgc tcaatacggg tcactcgccg aatctcacct gttaacccga
    50221 tctcgcctaa gaaagcttct tgagggctgg ttggtttttc cttataactg gatgcgatag
    50281 ccacagcgac tgccaaatca atggcaggtt cgtctagttt gacaccacca gccgacttga
    50341 ggtaggcatc ttgattttgt aagagtaaac cacaacgctt ttctaaaact gccattatta
    50401 ggcttacgcg attgaaatca agtcccgtag tggttcgtct ggcattgcca aaaacggttg
    50461 gtgtcactaa agattgcacc tctgctaaga taggacgact tccttccatc gttacaacga
    50521 cagccgatcc tgttgcccca tctaagcgtt cctctaaaaa gacttgacta ggatttaaga
    50581 cctctaccaa gccgccagac tgcatttcaa agatgccaat ctcgttagtg gagccaaagc
    50641 ggtttttcac agcccgaaga atgcgaaagg tgtgatgcct ttccccttca aagtagagaa
    50701 cggtatccac catgtgttct aacatacgag gcccagccaa ggttccttcc ttggtgacat
    50761 gacccacaat aaaggttgca atgttgttcg tctttgcaag ctgcatcaat tctgcagtga
    50821 cttctctcac ctgactgact gatccttgaa cacctgtaat atcagggctc atgatggtct
    50881 gaatggagtc aataattaaa aaatctggtt taatattttc aatttctgtg cgaatagctt
    50941 gcatattggt ttcggcatag aggtaaaatt cattgtcaat atcacccaga cgctcactcc
    51001 gcagtttaat ctgctctgct gattcttccc cagacgcata gagaaccgtt cccttattag
    51061 cgagctgcgt agatacctgc aagagtaagg tggattttcc aatacctgga tcaccaccaa
    51121 ttaaaatcaa gcttccagga accacacccc ctccaagaac tctattaaat tcagacatgt
    51181 cagtctgtgt tctgtgataa ctaatattat ccacatcttt tagtttaacc ggtctggact
    51241 tttctccagc tagactaaca cgggcatttt tgacttcttt gacctctact tcttccacaa
    51301 aagaagacca ggccgagcaa tttggacagc gccctaaata ctttggcgac tggtagccac
    51361 actcctgaca gataaaggtt gcttttttct ttgccatagt aatctccctc ccctagacaa
    51421 gacaaaccca ctcttaaaga ataggtctac tgtcataagc atttttgtta aatcgcttta
    51481 ctgccctgtc gaaccaaaac cacctgttcg ttcacctctg gcttggtcac catcagcaat
    51541 taaaaaaggc ataaaaaccc cctgaacgat cctctcaccc acagctaatg tcacagggtg
    51601 atctgtaatg ttttgcattt gtgcaaaaat atgtccttca ttagcctcat ttccataata
    51661 atctgcatca atcacaccta ctgaattaat caggataatc cctttttttc gtggatttga
    51721 cgaacgatca tagaggtaaa gcacctcacc atcttgcatg taggccttaa cccctgttgg
    51781 taccaactta atctcccctg gggcaatggt tacagcctca gcaacagaca ggtcataacc
    51841 agctgcatga gtcgtctcac gttttggtag taaatcagga ttagtaaagc ttgaaacaag
    51901 ctcgaaacca cgaatttttg tcattatgat aggtatatcc tttcaagtca atcatatcct
    51961 cttattatag actattcgta aaagaatagc aaaaaggcgg tcaactttaa acggaccact
    52021 tttaatcatg ccatcacatc tcatccccac gataactaaa gcggatgtgg gtgaaatcat
    52081 tatccaaatc cttgtctgcc ataaaagctt tggcatcttt tttcacttga accaagtcaa
    52141 taccttgcat tttggcagca tagacacagc cacaatagca ttggcggtaa atgtcatact
    52201 cctcacacat ttcaacggat ctgcgataac cgttgttttt cttgaaatca cttggcaggt
    52261 atttggtggt gtaaaccttt tgcacatcga tgcccacatc attaatggtt tgcgaatttt
    52321 tatgaggact gatggttagg gcactggcaa agtagtcaaa acctagttcc actgcttttt
    52381 gagcggtctt gtctagtcgg taatcaaaac agacacggca acggtcccca ccttctggtt
    52441 cttcttctaa acctcgcact tgtcgaacgt actcattggg gacatagtcc gcttcgagaa
    52501 actgcacggt gttgcctgtt ttagcgttga actcggagac aaactgttgt gtcacatagg
    52561 ctctgcgatg gtattcgtcc ttagggtgaa tattagaatt ggcaaagtaa accgtaatgt
    52621 cagcaaactg cgtcaggtat tctagggtgt aagtggagca tggggcgcag cagacatgca
    52681 ttaaaatact gggacgcacg gattccttag cccaaacctt ggccatctgt tgcatgaccc
    52741 tgtcataatt tatcttttga ttgggattca tgttagcgag aatctcttgt aaatcaatca
    52801 ttgtggctac tttctaattg ttttttctct tgcttgctat tataccatat caggaaagaa
    52861 agcttgactg ctttattgtt caccaaaaaa ctccgctaag acgcggagct gataccatct
    52921 attcaaagac aaattgattg tgatacaatt cggcataaaa gcctttttga tgcaatagct
    52981 catgatgatt tccttgctca atgaccttac catctttcaa cacaataatg tgatcggcat
    53041 ttaaaatggt tttgaggcgg tgagcaatga caaagctggt tcgacctgcc acgatagctt
    53101 ccatggcccg ttgaatttta ctttcggtaa ctgtatcaac atttgaagtg gcctcatcca
    53161 aaatcaacac ttcagggtca gtcagtagtg tcctagcaat agaaatcaac tgcttttgac
    53221 ctgttgaaaa gacattgtca tcatctgaga cataggtatt gtaccctttt ggtaaggaca
    53281 tgataaagtc atgaatatgg gtcgcacgcg cagcagtttc aaccatgtct tgactaatgg
    53341 tctgatcacc aaaacgaata ttatccgtaa tggttcctga aaaaagaact gactcttgca
    53401 acacaatccc taccttttga cgaagactat ccaaatcgta gtcacgaata tcacggccat
    53461 caaaggtaat cgaacctgca tccacatcgt agaaacggtt aatcaagttc ataatagtgg
    53521 tctttccaga acctgtcggt ccaaccacgg caatcatttt gcccttgggt gcaacgattg
    53581 acacatctga taaaactttt tgcccaggaa gatacccaaa atcgacgtgg ttaattgcca
    53641 ctgcttcttt taagctggtg aacgctggtg cattttgtgg acgaacttct tcggtttcat
    53701 caaacatttc ttgaatacga tgagcaccgg taaaggccag ctgcaattct ccccaactag
    53761 acgcgatttg catcatgggt tggtaatatt gctgggaata ttgtacaaaa gtaaccacta
    53821 aaccaagcgc tgccgctgct ggcatagatt tgtcactgag gacaattgtt gaaccgacaa
    53881 aaatcacgat agccgtgtta ataaggctca ttccattcat gactggaaat aattgtcctg
    53941 agaacagacg gcgtttgaag gtggcttgtc gaaccctttc gttatgtttt aaaaaggctg
    54001 tcaccgtatc ttcttggaca ccttgtacaa taatagcctt ttgtcctgaa atggtttcat
    54061 ccataaaagc atttaaagca ctgacttctt gctgttggat attggtgtat tttcttgcca
    54121 aacggatgtt aatcactaaa aaaatgagag caactggggt tgatgcgatg gttaacaaag
    54181 ctaaacggct atcttgccta aacatcatcc agaccaggcc gatgtaaagg gcaatattag
    54241 tcaccacttg aaccaaggat tggttcagcg agttttgaat attatccaaa tcactcgtga
    54301 aacgagaaag aatctcccca tctttatggc ggtcaaaaaa ggcgacggtt aaacgttcta
    54361 atttaccaaa caagcccttg cgcatgcggt tggtcgaatg tgagacaaca cgtgtgagta
    54421 agaaactata tattagatta gctaaaacag taaagaaata agtcatcaaa agcttaaaca
    54481 tcacggcatt aaaagctgat aaatcagggc taaaatgtgt ctgaccactc atcttagcaa
    54541 cgtaataagc ctgaccgatt tttcccaact cagtcaagga ctcccctaag aagacaggag
    54601 cttttacttg taaataagtt gctaagataa cggcaacagc aatgacagta aacgagaaac
    54661 gatagcattt aaaataaaac cagaaaaaac gtgctgtttt catcactact cctcccttcc
    54721 tttttgtgtc tcataaattt cacgataaat cgcattatta gccaccaact ctgcgtgtgt
    54781 tccttctcca attaactgtc cttggtcaag gactaagatc ttatctgcct taactaccga
    54841 tgaaattttt tgagcaacaa taactgtagt tgtaccttct aatttatgac ttaaggcttc
    54901 ttgaacgcgt ttttcagatt ttgcatcaag agctgaagtg gaatcgtcta atataagaat
    54961 tttgggatga ttaatgacac cacgtgcaat agacagacgt tgcttttgtc cgccagacaa
    55021 gttactacct cgctcttcaa cttggctctc ataacggcta tccatacgat caataaattc
    55081 ttttgcttgc gcgatttgag cggctttttg catagcatca atatcagctt ttgctgagcc
    55141 ttggcgaagg ttatcagcta tcgttcctga aaagagaata gccttttgca acacgatgga
    55201 aaccgattgg cgcagggttg tttgacttag ggttttaatc ggctttcctc ctaacaaaat
    55261 ttggccatct tgaggatcaa ataagcgagg gatgagctgt gcaagcgttg atttcccaga
    55321 tcctgttgca ccaacgatgc cgaccatttg cccagattca atagcaaaag aaatgtgctt
    55381 caaggttggc tcatcatcat taggatatgt aaaactaaca tcgtcaaata caatgcttcc
    55441 tgagatggtt tcttcctttt cattttcaaa tgtcatggca ggttctgtag acaatatttg
    55501 cctaattcta gccatagaaa caaaagcacg actaacttgc atccccatgg aaccaaccac
    55561 aataattgaa aacataattt gcatcatata agtcatgaaa gaggcaatat tcccaatcac
    55621 tgttggatct gtttcgacca tggtcgaaac aacatttata gaaacataaa ctgccaaata
    55681 agacaccaac attaaagccg gctgcattag tgaaaaacca taaccaatag ataaatttaa
    55741 cgccaaaaga tcattggacg tttctttaaa tttagtgtat tgttgttgtt cttgtacaaa
    55801 tgatttaacc acgcgaacgc cacggagatt ttccttagcg atgcgattaa tcttatccat
    55861 taaccgttga aatttaccaa aacgaggacc catttgacgc atcaccaaac ccatgatgag
    55921 ggcaattaag ataaccatta caacaatgac ccaccataat tgagggaatg tctgaactgc
    55981 cataataaac gcgccaataa aaaggatcgg caaacgaaag agaatttgaa acatcatcat
    56041 gactaaactt tggatttgat tgatatcatt agttagccta accacaagat taccagcatt
    56101 aaaagcttca atattggcat atgaaaagtc ttggattttt cgaaaggtct tctccctcat
    56161 atcagcagat acaccttgag ctattttagc agctaaaacc gtattaatcg ctccagacat
    56221 taaccctaaa agggcaataa taagtaatgg tttcccaacc tcataaattt tctcttgatt
    56281 attttgaatc acagcgctta gcacagattt aagataatgt ggttgcaaga gactacttgc
    56341 aacaacccca ataaccataa aaaatgagcc taaagcatac catttataac gtaagattgc
    56401 gtctttaagc atcgttgtct cctttataag ttagcatatt ttgcttgagc tgttcaatta
    56461 cttttttcgc tgtcagcaac tcttcttttg taatttcttt taaaaaatag tgctcgatat
    56521 ccttacgaca ctcccgcaac aaaggcaatt tatctctacc aacctgcgct aacactactt
    56581 gcttataacg cttatccact tgggaaggca tcacattgat aaacccattt ttgaccatac
    56641 gtttcactaa atgactagca actgactttg agatacgaag ttgtttttca atatctttga
    56701 caaatatttc ttgattttgg tgtttagcta aaaaaacaag aacataacct tgaggacctg
    56761 ctagatgttc tacatcatat ttttttgcaa tctcatcact aatttgttcg atttgatgta
    56821 tcaattcacg taaatcacct atcacttgtg acatctcttc cctccttatt ttctttaata
    56881 gtttctatga taagtatttt agttctctag ataactattg tcaattaaaa tatctttatt
    56941 tttaaaaata agtttccgaa cttaaataac taaaaagaac agggcaaagc catgacaaca
    57001 gctcacctga tgaagcttat taaacaacat tattaatata gctagctctc ttgggaacgc
    57061 cttattagcc aagaccatat accttttcta atagagctaa tcaaggtgtt atgttataat
    57121 agagtgacta gctcttgaag gagaatttag aatgacaaaa caaaaagtag ctattttagg
    57181 acctggttct tggggaactg ccctttctca ggttcttaat gacaatggcc atgacgtccg
    57241 tttatgggga aatattcctg atcaaattga agagataaat accaagcata ccaatagaca
    57301 ctacttcaaa gacattgtgt tagacaaaaa cattacggca actcttgatt taggtcaagc
    57361 cctatcagat gttgatgcgg tgctttttgt tgtgccaact aaagtaacac gattagttgc
    57421 tagacaagtt gccgccattt tagatcataa agtagtggtc atgcatgcct caaaaggtct
    57481 agaaccagag acccacgaac gtttatcaac tatcctagaa gaagaaattc cagctcattt
    57541 tcgtagtgaa gtggtcgttg tatcaggtcc tagtcatgct gaagaaacta tcgttcgcga
    57601 tattactctc atcacggcag cctcaaaaga tattgaagct gcaaagtatg ttcagtccct
    57661 ctttagtaac cactacttcc gactttatac caataccgac gtcatcggag ttgaaacagc
    57721 tggtgccctc aaaaacatta ttgctgttgg agcaggagct ctccatggac ttgggtatgg
    57781 agataatgcc aaagcagcgg taattactcg tgggttagct gaaatcaccc gtctaggcgt
    57841 caagcttggt gctgacccgt taacctacag tgggttatct ggtgttggtg atctaattgt
    57901 aacaggtact tctgtccatt cacgtaactg gcgggctgga gctgcccttg gacgtggtga
    57961 aaaattagaa gatattgaac acaacatggg tatggttatc gaaggcatcg caacaactaa
    58021 ggtagcctat gaaattgccc aagacctcgg agtttacatg ccaattacaa cagctattta
    58081 taagtccatc tacgagggcg ctgatatcaa agaaagcatc ctcggcatga tgtcaaatga
    58141 atttcgttct gaaaatgaat ggcattaagc cattcgtcac taaaataaga ggaggtgctg
    58201 atgaccaaag tcagaaaagc tattattccc gcagcaggtt taggcacacg ttttttacct
    58261 gcaaccaagg cccttgccaa ggagatgcta cctattgttg ataaaccaac tattcaattt
    58321 atcgttgaag aagctctaaa atcaggtatc gaagaaattc ttatcgttac tggaaaatcc
    58381 aaacgctcca tagaagatca ttttgattct aattttgaat tagaatataa tttacaggct
    58441 aaaggaaaaa tcgaacttct aaagttagta gacgagacca cttctattaa ccttcacttt
    58501 attcgacaga gtcatccaag aggattagga gacgcagttc ttcaagcaaa gacctttgtt
    58561 ggcaatgaac cttttgttgt catgctaggt gatgatctga tggatattac aaatccaaat
    58621 gtaaaaccat taaccaaaca attgatcgat gactatgaag aaactcatgc tgcaactatt
    58681 gccgtcatga gggttcctca cgaggatgtc tctaactacg gtatcattgc tcctcaagca
    58741 aaagcagtaa aaggactcta tagtgtggat acttttgttg aaaaaccaca acctcaagat
    58801 gcacctagtg atttggcaat tattggacgt tacttgctca caccagaaat ttttagtatt
    58861 ttagaaaaac aagaacctgg tgcaggcaat gaagttcaac tgactgatgc tattgatacc
    58921 ctcaataaga cacagcgtgt ctttgctcgt gagtttaaag ggaaacgcta tgatgttggc
    58981 gataaatttg ggtttatgaa aacttctttg gattatgccc tgaaacaccc tcaagtcaaa
    59041 gatgacttga aagcctacat catccaactg ggcaaagcac tagagaaaac aaaaccataa
    59101 aaataaggag ggaaaatccc tccttatttt tatggttttt gagtcgtttc atcctactag
    59161 ccatctgaga agtaacagtg ctaaccactc acttattttt ataaaaaccc aagcactaac
    59221 acaccaacca aaataatacc atagctcact aaagccaaca ttcgcttggt tttcttcact
    59281 gttatcactc tcatcttagt tggaaatact atactcagta acacaccacc cacgacaccg
    59341 ccaatatggc ctgccatact cacgtttggc ataaaaagat tcatcagtaa attgactaca
    59401 attaacgtct ggtatgactt gcccaaatct ttcaacgctt ggttcttacc caaaaaactc
    59461 aatactacaa tagcagcaaa aagtccaaat aacgacgtcg atgctcctgc agctactgtt
    59521 tcaggactca accaaaaagt aaaggcattg cccataacac ctgataaaac ataaagtaat
    59581 aaaaaacggc gtgaacccca gaggtcttcg acaatttgac caacaaaata tagtgttaac
    59641 ccattgacaa aaaaatgacc aaagccgata tgaataaata tcggagtcac caatcgccac
    59701 aactgatctg gcattgcttt tactagtaat ccaaacatac ctccaacttg ataaatagct
    59761 tgagcaccag ttgctaaatg tccatatacc acttgcattg ctataaaaat taagcctgtt
    59821 aaccctaaaa ggaaaatagt aataggatat cgttttaata accgtgtcat ctaagataaa
    59881 cctcctaaac agcaatatca taaatcgttt gatttggcaa tcgtattggc ccgctgataa
    59941 gcttgattgc taacaacatc cactaataaa gcttgatcat tttgacgttt taagtctaca
    60001 tccatagttc gtaaagacta gaagagttct ttgagaatct cttttttcat catcttctta
    60061 tcataccata aattaggaaa aaggttcaag actgacaaga aattagctct ctcaaaaaag
    60121 attaaaaata aaggcattct aaagaatcat ttaagaaaac tttaaggtta ctatcgtaga
    60181 atgaaaacat cctaaaaagc ggtttaccta aaccgttggg agaataggtt ttccccttgt
    60241 tttccctttt cataaatctc ctgggcacct atcctcgtag gtgccctccc ttgtttaaac
    60301 ctgaccagtt aaccggctgg ttaggttttt aaatttaaaa agtcaacagc aaaaatatga
    60361 tatcacccgc gtgttcttag ctctctcaat tatgggctaa aaacacgcat caatcaatct
    60421 attgatgaag tttgctgctt tttgatgatc tttattgtga ctatactggt aatcctattg
    60481 attttagttg acttttacgg tagattcgct acaatatata ggattataca atttaattta
    60541 aggagagaaa aattacattg ttagattatt atttggaatc cgatattatt gaccagaaaa
    60601 tcctactggc tattttgttg acagaaaaag aactgacgat ggcagcagct tgttctcaaa
    60661 caactttgac tgctttaaaa ataaagcagt atcttcgaca atttaacgcc ttatttaaag
    60721 ggtacttacg tattgagctt attaaatctc gtatttattg tgaggtatta aatgataagc
    60781 gagaagcatt tttttatgat atttttgcct tatcagatac tctaaaagtg ttgacattct
    60841 tattactaga taatcccaaa cacaaatcaa tcgctgttta tacacgaaaa caagggatat
    60901 cccagtcaaa agcttatcgt cttattcata aattaaaaca ttatttacaa gacattggtt
    60961 taaatattgt agacaatact gttatcggag atgaacttaa aatacggtat ttgattgctc
    61021 ttttacataa agagtacggc attatattat atgatataca acctgcagac attgaaacga
    61081 ttcatgcttt tatctttgca gcacaaaaaa acttacaacc ttctgccttt ttagatagac
    61141 gcttcttgtt ttttgatgtg ctgttgatgt tgacttggaa gcgccatcgc tatcctgttc
    61201 atttgcctca tttagctctt tttgaacatc tcaaatcctt gcctattttt gacaatatca
    61261 aaaccattgc cgtggatgag ctagcacctc gaactcgtgt cacattttcg tctgatgatt
    61321 ttgactatct ctttttaatt tatttaacga ctgataactc cttcttgagt gggtactgga
    61381 caagtcatca gcgacaacaa ctttaccatt taatcaccaa agatcccgat tatcatttac
    61441 taatccatcg tcttcaggca ctagttggaa aatattccga tattcatgag catatcccta
    61501 acttaattcc ttttttcaaa agaactctct acaatttaca aactttaatt acctttgatg
    61561 gttattattt tgatcaatat caaggcaaca tgctactact agataagctt gaaaccgtta
    61621 tcaaagattg gctttatgac acaggccgtc aaggcagtat tagttctggt cacttacact
    61681 tgatgtgttt ataccttgag cagatcttgg aaagctctat tgctccaatc aatattactg
    61741 ttattgagag ccaagaaact gttggtaacg ttataggtaa tttcatcacc tcaactatcc
    61801 cttcttacaa agttgaactg tcacgggtca atatcttatc tgataatatt tatccctatg
    61861 ataaacctgt agatctggtt gtgaccagtc agaagctctt gcctttctta aaagaactcg
    61921 gtgtttttcc caaagagacc cgcttgtttg gtttgagttt ggactgtatc caacagcagc
    61981 gcgaagattt aatcaaaacc atcttagcct tgcaccagaa ccactaccaa aaacgtttag
    62041 aagaactttg gagagttcct tcttaatttt ttgacagtcc ctcctagata cttttacagt
    62101 ctaggggggt ctttaaggtt gtcacttgca accttgataa gctctctttt taaccattgc
    62161 tatttgctta gaaacagaac tggaactgtt ttagcggttc aactcctctt gtaggatctt
    62221 cttccctttt ttgaaggttt tatctgaaat ttggcataat aaagtgcgct cccatgttct
    62281 ggaagcgcac tttattatcg cttttactta accttgtatc cacttgatga tggaagtgcc
    62341 gccccaattc ttagattgct tggctaactt ttaagctctt gtacaaggaa gaagtctttt
    62401 tgactatctg ttgattagag gcgagcatta agctctgctg ataatgcttc aaaacctggt
    62461 tttccaagaa gcgcaaacat attacgtttg taagcttcaa cacctggttg atcaaatggg
    62521 ttaacagcat tcatgtagcc tgaaacagcg atggcaagct caaagaagta aatagtgtaa
    62581 ccaagggtaa actcgtcttg agctggaaga gtgacaaaca tgtttggcac gccaccatct
    62641 gtgtgggcaa gaagcacacc atctgtggct ttcttattaa caaaatcaac gtctttacct
    62701 tgaaggtaac caagaccatc taggtcttct gctaattcag gaatgataac atttttacga
    62761 gggttatcaa cacgaataac tgtttcaaag aggttacggt aaccttcttg gataaattga
    62821 ccaagtgagt gaaggtctgt tgagaagtta gctgaagttg ggtaaatccc tttttgatct
    62881 tttccttctg attcaccagc taattgtttc caccattcgc caaagtactg aagagatggc
    62941 tcgtagttag ccaagatttc agtgatgtag cctttacggt aaagcacatt acgaacagca
    63001 gcgtattggt aagcaatgtt ttcagagatt ttgtctgaag aaaggtcttt acgagcagca
    63061 tttgcccctt ccataagagc agtaatatct gcacctgatg cagcaattgg aagcaaacca
    63121 acagcagtca agactgagaa acgaccacca acattatctg gcaccacaaa tgtttcccag
    63181 ttgttagcgt cagcttctaa cttaacagca cccttaactt tgtctgtggt agcgtagata
    63241 cgtttgttag cttcttcttg gccatatttt ttaaccaaaa gttctttgaa cacgcgaaaa
    63301 gcaatagctg gctcagtggt tgtacctgat ttagagatca cgtttactga gaattctttg
    63361 tcttgaacgt actctacaag gtcagctaag taagttgatg aaatagagtt tccagcgtaa
    63421 agaatttgtg gggctttgcg ctctttggca gtttgcaagt tagcaaaatg atgatttaaa
    63481 aaatcaatcg ctgctttggc accaaggtat gacccaccga taccgatcac cacaagaact
    63541 tcgctgtctg ccttaatctt ttcagcagca gtcaagatac gtgcaaattc ctctttgtca
    63601 tagttttctg gaagatcaag ccagcccaag aaatctgaac ctggtccagt tccttcacgc
    63661 aataacttgt cagcttctgt gacttggcct tgtaaaaaat caatttcatg ttgtcccgca
    63721 aatgactcga ggacttttga ataatcaaat gtaatatgtg acatgagttt cgctccctta
    63781 aaattttaag tttcattagt atgataacct tttctatcta atatttcaat ggtttttagt
    63841 ggttttcacc tgtgaaaaag ctaggttttt tgaaaaggtt ttcgtttttt cagacataaa
    63901 aaatgtgtca atgcaagcat attctcatca tagtggtttt ttaagagaaa ataggatttc
    63961 atatttccta aaaatatcat gaaaaaaggc cctgaggccg ctttaccgta ataacttatt
    64021 ttaaggattt tatcatttca acacgggtaa taaccccatc atgcatatga tcaatgcata
    64081 aagccacacc tacaaaggca attgaagccc caatcacctg taaatacttt ctcatgctat
    64141 cttcttaaca ttacttactc tcattatcta aaaatggcaa tcatttgaat acttaaaaag
    64201 caaagggggg agaatagcac tagtgcgttt ttaagtagat atcaaaatga ctaatttctt
    64261 cactattaaa aatcttatta tctttatacc ttttaaagaa actgtctcga cttaaaagct
    64321 tatcatcctt attaaataaa tcaacttcat aatgtttact atctttagta gcaaggaaaa
    64381 ggctccccga tgtataacgc gattccgaat catagatatt gtatttttcc attagaaatt
    64441 ttcttatttt aaaatcaaat tcctgtaaag taaactgttt cttttttatg cgaacagcct
    64501 cagagggcaa ataagtttct ttcccatctt gatttaaaat tcctacaaac tgtaatgatt
    64561 tatttccttg attcgaatgt tttacaatac caccataaat attatctaca taaggtgggg
    64621 aaaaattata agggagacca aaaacatcga ccttatcccc ttttataaac tgatcagtaa
    64681 tgcctaaaga tacaatagaa tcaacataaa gaatacattc cgatcttttt tgtttagtat
    64741 taatatgtat gctatttgta gtaacaaatg caatttctac attttcataa tcgcatgggg
    64801 taatattata ggcaaacctt aaacttcttt ttaaatcttt taaattttca tctgcataag
    64861 ctaattgact tccatagcta aaaacacatg ataatatgat aattgtcaaa atgtttgttt
    64921 tcatttaaaa ctttttatgt cctgttttat acctcttaac ctaaataaaa atatcaatcg
    64981 gtttcaacaa ctgatgacac tatagaagga ggagattact ttagtaaagt caacaaaaaa
    65041 agagcacaca cccgaaaatc gcttagggct gctggattcc tcccctgacc cacttcacgc
    65101 acaagtgttg ctccgcctat tattatatca tattacggac agtttgcaag ttttccaact
    65161 aagtaaaatc actttcaagt gagcatagct tcattttggg acaagatgga ctgaatacct
    65221 agaaaaatca tgaccctttc caaacacgat tttgcttttt gaaaatcttt ccctctcccc
    65281 attccagcta ttctctatgt tataataaga ttattagatt agaatgggag ttatgatgaa
    65341 acaaaagatt aaaatcttaa cagtaattgg ccttatgaca gttggtatgt ctgcgtgtca
    65401 caacactagc aaaccttcta ataccgattc tgttttttct ttaaccggaa aaaagcgcca
    65461 acaaatcgtc aaacaagtaa gacaacggta ttatttccaa caattatcca aaaccgaaca
    65521 agaaaattac ctcactttat acgatagtct agcacaattt cgtgaaatca tcagtttgac
    65581 gccggcttct aaaaaaagct taattaaaac gattgacgcc tttgtgatgg ataatcccga
    65641 attttactgg attacatctg ccgattatcg atttgagttt tctgatcaaa cggtctttgt
    65701 cacctttcct atcccagaag atgccaagaa cgtttaccaa gacttgcaag caatcggtaa
    65761 tgatatcgtc gcaaacacgc catcaaagga tcgctatgag caggtcaaat atttttatga
    65821 ggtcatcatt cgagacacag actacaataa aaaagccttt gaagcctatc aatcaggcag
    65881 tcaagcccag gtcgcttcta atcaagacat taaaagtgtt tttattgatc atttatctgt
    65941 ttgtaatggc tatgcccaag cctttcagtt tctttgtcaa aaagctggca ttccagtcgc
    66001 ctatatccgt ggaactggta catctcagca acctcagcaa tcttttgcac atgcatggaa
    66061 tgctgttcaa attaataaca cttattatgg cgttgatgtc acttggggtg accctgtttt
    66121 tgataaccat ctctcacatc aaaagcaagg aactatcaat tacagttttc tatgcctgcc
    66181 agattatcta atggccttat cacatcagcc aagcaaagac attgctttta atactaaaga
    66241 acgttttgaa aatgtttgga ctataccttc ttgtacagat gattctttgc tttattctaa
    66301 acgtcatcaa agctatatct caacgtttga cagtgacgcc atccttgcaa gccttgaaaa
    66361 tcagttgtta aatagacaag agcaactatg tctacaattt gctcatcaag atgattatca
    66421 acagatggta actgatttaa cgacgaatca aacaggttat cataaccttt tcaatcagta
    66481 ttggaataat tatacgggct ttacctatgg actcttacca gagactctta gtatttcatt
    66541 tgctagtagg aactaggttt agacacatga aacacaaagt taagatgatt atctttgtca
    66601 aacattacaa gaaattgata tactgacaca attgaaggcg attattattc taaccgtctt
    66661 caatagatat aaccttttca gattgagcac atctaatgct tgtaacatta gcaacagtga
    66721 tgagctcttt tttaatgctt tagcaaaata tatcagctaa gacaaaagat taaataactc
    66781 aagccatgcc tataagtcac ttgtttttaa tccctgtgtc agtgtcaatc tacgccacag
    66841 ataactgaca gctctgagcc ttagaagtct gagctggata agtttactaa aaacaaaaat
    66901 aaggtgagcc attactatct gtttttagat agtaaatctt tttaagaatg actaactcat
    66961 ctagtcatca cgtgttttat ttttcgattg ttttcacctc gattgggtct gaaaaagagc
    67021 gcttctagta cttggaaacg cccttttgta tattctatct tcgtatgttg aatctaacaa
    67081 ccattggaaa aatggtctaa cgtttcactc attattttga caggttaccc ttcaccaaaa
    67141 atgcatgtga taacaagctc cttaaaactc taatcaaagg gatctgactg ttccttgatc
    67201 aaatgcttag ctattttttt gcgctctcgc ccttggcgga aaaaggtttg catgatatta
    67261 gcacagtctg ctgctagcag gcctcgttca acttgtacgc ggtggtttaa gcgctcatct
    67321 gtcaaaatct ggtaaaggct gtctgctcca ccaaattttt gattgctggc tccataaata
    67381 acatgcggaa tccttgctag cccaatggca ccactacaca tcacacaagg ctcgatggtc
    67441 acaaagagtg tggtgtctaa taaacgccag ttgccttcat gagcgttagc ctcgttaatg
    67501 gccatcattt cagcgtgcat aatggcttgg ttactctctt cacgcgcatt gtgcccacga
    67561 ccaataatct caccatcctt gacaatgaca cagccaatgg ggatttcagc tttctgtaag
    67621 gatttttcgg cttcttttaa agcctcttgc atgaaataag tttgctcttc taaactatat
    67681 ggcatcaaga aagactcccc aagttccaag atttgtgtgg taaggcaagg tcaacaggaa
    67741 tattacctgc ataggcttgt gtttcttgcc tcaacacttc caagtgattg tcaggatcaa
    67801 taccttctgg aggaaccggt ggcatgtggg tcaagacaag cttcttaaca cctgcctggc
    67861 tagccattag tcctgcttcc ttactggtca aatgggccat atgattttca ttgccttcgt
    67921 ataagtaaac atctgctaag aaaaggtctg cttcttttgc gaagtctgcc aatccttcaa
    67981 aataaccact gtctccagta aagacaagta cctgaccagt cgctctttct acaattcgaa
    68041 aggcataaca cacaactgga tgaactgttt taagaaaggt aatgtcaaaa ggaccaatct
    68101 gttcaactcc agtgacatca taggctctac cttctgaaac atcaggaatg cttaacttag
    68161 caaattcgtg tcggtcctca tcatgcccat aaatgggcaa tactttaggc tgccacaggt
    68221 gtttaggata aagttgaaaa taatggcgca acacgcctaa atcggccacg tggtctggat
    68281 ggtaatggct aataatcact gcatccaaat cgagaggact gatttctttt tccagctcat
    68341 tgagcgctcg actaccagca tccatcaata actgaaagcc gtcatgtcct gtgaccaaat
    68401 aagaggttgt gcctgcatct tgatagggat aggctcccca agaccctaag gtggttaatt
    68461 tcatctcaaa cccttctttc ttgctttaac tcttattata acaaaaatta gtgtcaaaga
    68521 ggattgagac ccaccaaaaa caggtcctag cagataaatc tgccaagacc tgttcaacac
    68581 aacttaacta tttgatgatt agattactat tttgtattca aaaaactatc tttgtattta
    68641 acagcgataa tggtcgcaat gattgctttg atggtatctc ctggaataaa gaccaagttt
    68701 gacaaaagcg atgtcacaag agacatgcca gtgtaggctg ccaaccagat agcacccaga
    68761 acatcaacca gtacaactcc tgagattaaa agagctagga atgccaatgg cgtgcttttt
    68821 gagaaccagt tgcggtaaag taatgaaaag acaataggca caagaaggta ggcaataaca
    68881 tagccagcag atggccccat caatactgga atggttgttc gtgagcctga gaaaacaggt
    68941 aagaaaaggc caatcactaa aaataagaaa acagataagg ttcccttttt accacccagc
    69001 atcaatccag ccaacatgac acctaagttt tgtaaaacaa tagggactgg gataaagcct
    69061 agcgggatag caggaataaa gcctaaaata ataatcaaag tggtcatcat agccacctta
    69121 acaagttctt tggttgtaaa catctgttac gactccttta aaaaatagat atccttgtgc
    69181 ctatgacagg ctggattaaa gggactttga caataaggac agcatccaca accatactca
    69241 gctaggctca agagctttcg acaatgccca caaataacag gagatgtttc ttgatgtcct
    69301 gttggcacaa aggcatgatc tgttaactgg tcatggcaat ggtagcaagc ataataagtc
    69361 tgacaaaaag cacatttgag cccaacaata tcaagcgggg tatgataatg taggcaccga
    69421 tactcttggt caaggtcaat tccaaaacag tccgtcatat ctcatcctct tgtaacagtt
    69481 taatcaaaag catatgattt gtcaactcta tttttacaaa agggtaactg ttttctgaaa
    69541 aagaacacgt aaaacaagaa cttcccttta aagagggacc tatcagttga aagcaatagg
    69601 acatccgtct atacaaaaaa actgaaacca aagacgatag atcgccctca gttccaggat
    69661 catagcattt ttaattagaa attacggttt gatttattat aaccataccg ttcaagaaaa
    69721 tcttggcgga attcaagtaa gttatcgtcc ataatagcct gacgtacttt tttcatcaag
    69781 ttaaccaaga aataaaggtt gtggtagctt gtcaaacgaa taccaaaagt ttcgtccgct
    69841 tttagcaagt gacgaatgta ggcacggcta tagttttgac aagtgtagca gtcacagtca
    69901 tgatcaagtg gggtaaaatc ttcagcgaat ttggcatttt taatcacaag acgcccctca
    69961 ctagtcatgc aagttccgtt acgggcgata cgcgttggca agacacagtc aaacatatct
    70021 acaccacgaa tcacaccatc aatcaagcta tctggtgctc ccactcccat caggtaacga
    70081 ggtttatttt ctggtaagag gggagtcgta aagtcaagga cggcattcat ttcttcatgg
    70141 ctctccccaa cagctaaacc accaatagaa taacctggga aatccatagc caccaaatct
    70201 gccgcagact gacgacgaag gtcctcaaat ccagctcctt gtacaattcc aaataatcct
    70261 tggtcatgag gacgacgatg ggcttttaag ccacgttctg cccaacggct agtacgttca
    70321 atggattttt tcacgtaatc ataaggttgg taaaattgcg ggcactcgtc aaaactcatc
    70381 atgatgtctg agccaaggtt attctgaatg gaaatggctt tttcgggtga tagaaacatt
    70441 tttgagccat tcaagtgatt tttgaaagtc actccttctt cagtgatgtt tcgtgaatcg
    70501 gctaaagagt aaacttgaaa accacccgaa tcggttaaga ttggctgatc ccagttcata
    70561 aacttgtgca gaccaccaga gcgagcaatc aactcatctc ctggacgcaa ccaaaggtga
    70621 taagtgtttg ataaaataat ccctgatcca attgctttta actcttctgg agactgtgtt
    70681 ttaacagtag cctgagttcc cactggcata aacattggtg tcggaaaggt cccgtgaggg
    70741 gtgataattt cgcccaagcg tgcaccggtg tgcttttctg ttttgataag gcgatactta
    70801 atcggataat ctgtcatgtg ctttcttcct tgctatcaag ataaacagtc ttgagctttt
    70861 ttgatctgcc tgagtttcca tcataatgaa gggcttcaag atgacatgtt gttctagcgc
    70921 ctatcatttt atcaaaaaat ccataatttg tcatgctttc ccaaaaaagt atagtcaaaa
    70981 taatggttag ctgtctcatt ttgttttatg gtataatgaa ataaaggagt ctgttatgtc
    71041 aattaaagca atcaaaggcc aggctagaga cacgttaaaa aatctctctg gcaagtattt
    71101 actattttta attccaaccc ttctctttat gtttcatttc ggtattgaga ttcatcaagg
    71161 ttacgtccta tctagtggta ttgaagtctc gctagcggct agttatttcc cattactact
    71221 aggagttatc cttagccttt tcattttatc agcatccttt acaatgattg atgttgttag
    71281 gcattttcgt cagaaagttt cttttgccga atcaacaaca gccttctcaa aagaattctt
    71341 tgggaacctg ctggttttag cgatcactaa gtggctattc tttctcatct ggtcactaat
    71401 ttggtttttt ggcctcttta ttttcctttc cggactttca gcgtttttgg tcaatgctaa
    71461 atcaggtggc tcaacagtca tttcactgat ctttttacta tttggagcag ttttgtcctt
    71521 aattggtttt ggtatttata tcaaccgtta ttacgcttat agtctatcag aataccttct
    71581 ctacgataag gttaaagagg ggacttatct aggagctata gccgtcattg aaacaagtgt
    71641 agctatgatg aaaggttata aatggaagct ttttttctta caacttagct tcaccggttg
    71701 gtttctactg aatattgtta cttttgggtt acttaatatc tatcttttac catattttac
    71761 aacagccaat gtcatttttt atgatcaact caagaaacgt ttcaaagaca aagatgaccc
    71821 aattgaagga gaacatttaa gccttcatca gcttaataaa aagcctactc ctatgatgta
    71881 tgacgatcaa aaagactaaa taaatcctcc atccataaaa atagcccttg aactgcaccc
    71941 caaaagttag acacaaaatc taacgattgg ggtgctattt ttttatgaaa tttaatcaag
    72001 aaacgaaagt taagatttat gagttacggc aaatgggaga gtccattaaa tccatatcaa
    72061 aaaagtttga tatggcagaa tccgatctca aatatatgat tcgcttgatt gataggtatg
    72121 gggtaaccat tgttcaaaaa tgtaagaata attattattc tccagaactg aagcaagaga
    72181 taataaatga agtcctgatt gacggccaat ctcagaaaca aacatcctta gattatgctt
    72241 taccaacttc tagtatgctc tcaaggtgga tagcgcaata caagaaaaac ggttatacta
    72301 ttcttgaaaa accaagaggg agaccgagta agatggggcg taaacgtaag aaaaatttgg
    72361 aagagatgac agaggttgaa cgtcttcaga aggaattaga atacc
//
LOCUS       DATXQK010000005        70639 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.005, whole genome shotgun sequence.
ACCESSION   DATXQK010000005 DATXQK010000000
VERSION     DATXQK010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 70639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70639
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.005"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            13..330
                     /locus_tag="VC394_000311"
     CDS             13..330
                     /locus_tag="VC394_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEP5993810.1"
                     /translation="MKTKKISAVLEKVGLSSGYLNQKIYELSGGQAQRVAIARMLMKP
                     RKIILADEPTGALDGEIGKEIINLLLSETAENKYVIIATHDPAVYNKVDFIIDMKDIG
                     SKK"
     gene            327..818
                     /locus_tag="VC394_000312"
     CDS             327..818
                     /locus_tag="VC394_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5993811.1"
                     /translation="MKKKIYIGLIVIVGVFVVFLSGKQITQKETSNKPVVTLTVYTIS
                     SSDTQKWSKVKQFETKEAIYLVKVKETTSSEEVFSNIIADGMAVGFGVSEEDVKQFND
                     NLCSPVEDAKNNKLIGIEFINFSSVDKGFVVANFDYGKKAFNSQKTENKKLYKKLYEA
                     YKG"
     gene            1022..1552
                     /locus_tag="VC394_000313"
     CDS             1022..1552
                     /locus_tag="VC394_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEP5993812.1"
                     /translation="MGIEKTVSELADILGVSRQAVNNRVKSLPEEDLDKNEKGVTVVK
                     RSGLVKLEEIYKKTIFDDEPISEETKQRELLEILVDEKNTEITRLYEQLKAKDAQLAS
                     KDEQMRVKDVQIAEKDKQLDQQQQLTAKAMADKETLKLELEEAKAEANQARLQVEEVQ
                     AEVGPKKGFLTRLFAK"
     gene            1601..2188
                     /locus_tag="VC394_000314"
     CDS             1601..2188
                     /locus_tag="VC394_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEP5993813.1"
                     /translation="MKHLDTYIAFDLEFNTVNDVSHVIQVSAVKYDHHKEVDSFDTYV
                     YTDVPLQSFINGLTGITSDKIAAAPKVEEVMAAFKNFVGELPLIGYNAQKSDLPILAE
                     NGLDLRDQYQIDLFDEAYDRRSADLNGIANLRLQTVATFLGIKGRGHNSLEDARMTAA
                     IYKSFLETDTNKAYLSQQEEVTTDNPFAALGDFFD"
     gene            2341..3069
                     /locus_tag="VC394_000315"
     CDS             2341..3069
                     /locus_tag="VC394_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEP5993814.1"
                     /translation="MSQFYSTGELANLAGVSIRTVQYYDQRGILIPTALTEGGRRLYT
                     DSDLEQLRMICFLRDLGFSIEQIRKVLAEENAAQVLELLLVDHIATAKEDLAAKEQQV
                     DIAVKILDRLRKQDPQSLDFLMDISLSMKNQKAWKKRFITLIVCLTVETLLFVGGAYF
                     LQLTVWKYLLIPVFLLVLLGTLYWYYQNICYLCPSCHQTFEAKWTEFALAGHTPRTRK
                     LTCSHCHQKSYCLELAKEDTVSRG"
     gene            complement(3126..3425)
                     /locus_tag="VC394_000316"
     CDS             complement(3126..3425)
                     /locus_tag="VC394_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016503193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEP5993815.1"
                     /translation="MMNMQNMMKQAQKLQKQMEQKQADLAAMQFTGKSAQDLVTATFT
                     GDKKLVGIDFKEAVVDPEDVETLQDMTTQAINDALTQIDEATKKTLGAFAGKLPF"
     gene            complement(3618..3992)
                     /locus_tag="VC394_000317"
     CDS             complement(3618..3992)
                     /locus_tag="VC394_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP5993816.1"
                     /translation="MSAKKTFFASNLKYLRLKKNMEQLELANLLGRKSSSSISEWEKG
                     KYTPKSGLLSDIAAIFDVSLTALMEEDLTLESKGVSYQLPQRELAMISEQLSENNYTK
                     WVDFAQILLEQQAKEDRELDNH"
     gene            4162..4383
                     /locus_tag="VC394_000318"
     CDS             4162..4383
                     /locus_tag="VC394_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP5993817.1"
                     /translation="MTKSTLKELRTTKKMTQQELSHLTGISVRTIARYEKDTKKLRRA
                     KYEKLKGIAQALAVSVDDIFLGIDSEFMN"
     gene            complement(4463..6745)
                     /locus_tag="VC394_000319"
     CDS             complement(4463..6745)
                     /locus_tag="VC394_000319"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEP5993818.1"
                     /translation="MRYNQFSYIPTSLERAAEELKELGFDLDLQKTAKANLESFLRKI
                     FFHYPDSDYPLSHLIAKNDMDALSFFQSEQELSKEVFDLLALQVLGFIPGVDFTEADA
                     FLDKLAFPIHFDETEIIKHIHHLLATRCKSGMTLIDDLVSQGMLTMDNDYHFFNGKSL
                     ATFDTSQLIREVVYVEAPLDTDQDGQLDLIKVNIIRPQSQKPLPTLMTPSPYHQGINE
                     VANDKKLYRMEKELVVKKRRQITVEDRDFIPLETQPCKLPIGQNLESFSYINSYSLND
                     YFLARGFANIYVSGVGTAGSTGFMTSGDYAQIESFKAVIDWLNGRATAYTSHSKTHQV
                     RADWANGLVCTTGKSYLGTMSTGLATTGVDGLAMIIAESAISSWYNYYRENGLVCSPG
                     GYPGEDLDVLTELTYSRNLLAGDYLRHNDHYQELLNQQSQALDRQSGDYNQFWHDRNY
                     LKNAHQIKCDVVYSHGLQDWNVKPRQVYEIFNALPSTINKHLFLHQGEHVYMHNWQSI
                     DFRESMNALLCQKLLGLANDFSLPEMIWQDNTCPQNWQERKVFGTSTIKELDLGQELL
                     LIDNHYGEDEFKAYGKDFRASKAALFKGKANQALIDILLEEDLPINGEIVLQLKVKSS
                     ENKGLLSAQILDYGKKKRLGDLPIALTQSSIDNGQNFSREPLKELPFREDSYRVISKG
                     FMNLQNRNNLSSIETIPNNKWMTVRLPLQPTIYHLEKGDTLRVILYTTDFEHTVRDNS
                     NYALTIDLSQSQLIVPIASN"
     gene            complement(6767..7426)
                     /locus_tag="VC394_000320"
     CDS             complement(6767..7426)
                     /locus_tag="VC394_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEP5993819.1"
                     /translation="MNHHILQRYIDNHNFPIIEKSYHKYLTFESLEEDFTYILKDGIV
                     KQSVLSKYGMEFNLRYVTGLEITSVLNTGYSKDMGEPYNVRIESEKASFYKVRRSAFL
                     KDINEDIELQGYVKDFYHNRLQKSMKKMQCMLTNGRIGAISTQIYDLMTLFGEELPNG
                     QILINFVITNEELGKFCGISTASSVSRILKQLKEKNIIRIDKQHIIITNLDKLKDNIV
                     F"
     gene            7592..8788
                     /locus_tag="VC394_000321"
     CDS             7592..8788
                     /locus_tag="VC394_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEP5993820.1"
                     /translation="MEEKLFNKHFVAITVINFIVYMVYYLFTVIIAFVATRELGAQTS
                     QAGLATGIYILGTLLARLIFGKQLEVFGRRLVLRGGAIFYLLTTLAYFYMPTISMMYL
                     VRFLNGFGYGVVSTATNTIVTAYIPARKRGEGINFYGLSTSLAAAIGPFVGTFMLDNL
                     HIDFRMVIVLCSVLIGCVVVGAFAFPVKNMSLNAEQLAKTKSWTVDSFIEKKALFITA
                     IAFLMGIAYASVLGFQKLYTSEIHLTTVGAYFFVVYALIITITRPAMGRLMDAKGDKW
                     VLYPSYLFLAMGLFLLGSVSSGGSYLLSGALIGFGYGTFMSCGQAASIQGVDEHRFNT
                     AMSTYMIGLDLGLGAGPYLLGLIKDLALGSGVASFRHLFWLAAVIPLICTLLYLLKTK
                     TRQVLS"
     gene            9290..10138
                     /locus_tag="VC394_000322"
     CDS             9290..10138
                     /locus_tag="VC394_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEP5993821.1"
                     /translation="MEMTWTVKYITEFIATAFLIILGNGAVANVDLKGTKGHNSGWLV
                     IAFGYGLGVMMPALMFGNVSGNHINPAFTVGLAVSGLFPWAHVLQYVVAQLLGAIFGQ
                     LVVVMVYKPYFMKTENPNHVLGSFSTISSLDNGQKDSHKVSYINGFLNEFVGSFVLFF
                     GALALTKNYFGVELVGKLIEAGYDQTTAATQISPYVTGSLAVAHIGIGFLVMVLVTSL
                     GGPTGPALNPARDFGPRLLHHFLPKSVLGQAKGDSKWWYAWVPVVAPILAAIVAVAAF
                     KYLYIR"
     gene            complement(10474..11370)
                     /locus_tag="VC394_000323"
     CDS             complement(10474..11370)
                     /locus_tag="VC394_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEP5993822.1"
                     /translation="MNEMLILRLIQALLVSAMLFIFFMLVKHLKKNKINPFKRFWTGF
                     WIGLLTDALDTLGIGSFATTTTCFKLTKLVTDDRQLPGTMTVAHVLPVLIQSLCFIFV
                     VKVEVLTLLAMAAAAFIGAYLGTHITKNWHAPTVQRILGSLLIIAAIIMIIRIIYHPG
                     EHLSDTIHGLHGIWLFVGIGFNFIVGVLMTMGLGNYAPELIFFSLMGLSPTVAMPVMM
                     LDAAMIMTASSSQFIKANRVSWDGFAGIVSGGIIGVLLAVFFLTNLDINSLKLLVIAI
                     VFFTGGMLIRSSFTSHKQQTNR"
     gene            11668..12420
                     /locus_tag="VC394_000324"
     CDS             11668..12420
                     /locus_tag="VC394_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEP5993823.1"
                     /translation="MTLMENITFKTPVLRVNDRDLNIAFYQNNLGLRLVSEENAIAIF
                     SSWGEGQECFVIEESPSVRTRAVEGPKKVNTIVIKTNQPKEIEQLLAHGAHYDALFKG
                     QNGYAFETISPEGDRFLLHAEQDIKHLQGTDLPSLEKDATFKGLTQFKFDIIVLNVIS
                     EERSKAFYRDLFSDQLPITMDFIQEEGPDLAIDPHIAWDLEILEFQVSKDYDMKVLKA
                     TLEEDGHKVYIDKKHKVLVLSDPSQIEVWFTK"
     gene            12405..13355
                     /locus_tag="VC394_000325"
     CDS             12405..13355
                     /locus_tag="VC394_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEP5993824.1"
                     /translation="MVYEMTLAVIKCIENHLHKKVYKGASLALFQSGRWQEYHIGTID
                     GRRPVDANLVYDLASVSKVVGVATICNILLNNGTLALDDPLKAYYPSIVDATVTIRQL
                     LTHTSGLDPYIPNRDVLNAQQLRKALNHLTQKGNKNFYYTDVNFLLLGFMLEELFSES
                     LDQIFDKTIFTPFGMYHTSFGPRPEAVPTLKGVSDGEVHDPKAKILKKHSGSAGLFST
                     LADLESFSNHYLNDPFSDCLWRNYSQQTIERSLGWNLDGDWISHTGYTGPFLMLNKKE
                     QTAAIFLTNRTYDEDDKSKWLKERQLLYNALKHDLTTPVS"
     gene            complement(13535..15862)
                     /gene="pflB"
                     /locus_tag="VC394_000326"
     CDS             complement(13535..15862)
                     /gene="pflB"
                     /locus_tag="VC394_000326"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEP5993825.1"
                     /translation="MATVKTNTDVFEKAWEGFKGTDWKEKASVSRFVQANYTPYDGDE
                     SFLAGATERSLHIKKVIEETKAHYEATRFPYDTRPTSIADIPAGFIDKENELIYGIQN
                     DELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIRRA
                     RHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAITEIDEESIRLREEV
                     NLQYQALGEVVKLGDLYGVDVRRPAQNVKEAIQWVNIAFMAVCRVINGAATSLGRVPI
                     VLDIFAERDLARGTFTESEIQEFVDDFVLKLRTVKFGRTKAYDALYSGDPTFITTSMA
                     GMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDQLPETFRRYCMKMSHKHSSI
                     QYEGVTTMAKEGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNGGY
                     DDVHRDYKVFNVVEPITSEVLEYDEVMANFEKSLDWLTDTYVDALNIIHYMTDKYNYE
                     AVQMAFLPTHQRANMGFGICGFANTVDTLSAIKYATVKTIRDENGYIYDYEVTGDFPR
                     YGEDDDRVDDIAKWLMEAYHTRLASHKLYKNAEASVSLLTITSNVAYSKQTGNSPVHR
                     GVFLNEDGTVNTSQVEFFSPGANPSNKAKGGWLQNLNSLAKLEFSHANDGISLTTQVS
                     PRALGKTFDEQVDNLVTVLDGYFENGGQHVNLNVMDLNDVYDKIMNGEDVIVRISGYC
                     VNTKYLTPEQKTELTQRVFHEVLSMDDAAEAISSK"
     gene            16071..17165
                     /gene="dinB"
                     /locus_tag="VC394_000327"
     CDS             16071..17165
                     /gene="dinB"
                     /locus_tag="VC394_000327"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEP5993826.1"
                     /translation="MLIFPLINDTSRKIIHIDMDAFFAAVEERDNPALKGKPVVIGKD
                     PRETGGRGVVSTCNYEARKYGIHSAMSSKEAYERCPKAIFISGNYEKYRTVGDQIRRI
                     FKRYTDVVEPMSIDEAYLDVTNNKLGIKSAVKIAKLIQHDIWKEVGLTCSAGVSYNKF
                     LAKLASDFEKPHGLTLVLKEDALCFLAKLPIEKFHGVGKKSVEKLHDMGIYTGQDLLA
                     VPEMTLIDHFGRFGFDLYRKARGISNSPVKSDRIRKSIGSERTYAKLLYQETDIKAEI
                     SKNAKRVAALLQDHKKLGKTIVLKVRYADFTTLTKRVTLPELTRNAAQIEQVAGDIFD
                     SLSENPAGIRLLGITMTNLEDKVADISLDL"
     gene            complement(17258..17740)
                     /locus_tag="VC394_000328"
     CDS             complement(17258..17740)
                     /locus_tag="VC394_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5993827.1"
                     /translation="MISYEKVRQALKTSTIAIIILNGLGVVLSLMGFAGIFYLQSQLK
                     NEAFRAQLTTEQLAQLQSSMTPFMIFLSILNVLAIIAIIVFCAQNLSKLKQGLTVSYI
                     PYILGLILSVIGLVNQFTTTMSMVGTILILIQAALYGFAFYKAKTLNEKGDDTDQAML
                     "
     gene            complement(17831..20194)
                     /locus_tag="VC394_000329"
     CDS             complement(17831..20194)
                     /locus_tag="VC394_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEP5993828.1"
                     /translation="MEYVFTGTVDRIIFENQANFFKILLLAIEDTDSDIDDFEIIITG
                     TMADIIEGDDYTFWGELTQHPKYGQQLKLSRYQKIKPSSSGLVNYFSSDHFKGIGKKT
                     AEKIIALYGHNTIDHILEDPSKLETISGLSKANRQAFVAKLKLNYGTEQLIAGLVELG
                     LSNRFAFQAFEKYKEEALDLVKENPYQLVEDLQGFGFKMADALAENLGIESGSPKRFR
                     AALLHCLLEESINRGDTYVQARQLLDFAITLLEDARQVECDPAAVAEQLSELIIEGKI
                     KNSDTKLFDASLYFAEEGIANNISRLLDTPLSQSFSHDTIQTTIQAVQKDFAITYDQV
                     QQEAITKALTSKVFLLTGGPGTGKTTVIRGILQAYANLHQIDLDKKDLPILLAAPTGR
                     AARRMNELTGLPSATIHRHLGLNGDNDYQAMEDYLDCDLLIVDEFSMVDTWLANQLLG
                     AINSTTQVIIVGDSDQLPSVGPGQVLSDLLKVNSLPQIALQKIFRQSQESTIVNLADQ
                     MRRGILAADFRDKKADRSYFEAQAAFIPDMIQKIVLSAIKSGIPAEEIQILAPMYKGQ
                     AGINHLNQLMQELLNPLQGQTEFLFNDTHFRKGDKVLHLVNDAQLNVFNGDIGYITDL
                     IPAKYTESKQDELILDFDGSEVTYPRNEWLKLTLAYAMSIHKSQGSEFQVVILPITRQ
                     SGRLLQRNVIYTAITRSKSKLILLGEYTAFEYAIKHEGDKRQTYLIERFQEQSDLASS
                     QSTNSNPTENSQSDNDDFRLTPENYSTIDSMIGLTESDIALFFQKKS"
     gene            complement(20252..20845)
                     /gene="lepB"
                     /locus_tag="VC394_000330"
     CDS             complement(20252..20845)
                     /gene="lepB"
                     /locus_tag="VC394_000330"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEP5993829.1"
                     /translation="MKQFIKEWGPFTLFLILFGLSRLFLWQAVKVDGHSMDPTLAHGE
                     RLIVFNQARIDRFDIVVAQEEENGQKKEIVKRVIGLPGDTISYNDDTLYINGKKTVEP
                     YLAEYLKQFKNDKLQKTYAYNTLFQQLAETSDAFTTNSEGQTRFEMSVPKGEYLLLGD
                     DRIVSRDSREVGSFKKENLIGEVKARFWPLNKMTVFN"
     gene            complement(20856..21758)
                     /locus_tag="VC394_000331"
     CDS             complement(20856..21758)
                     /locus_tag="VC394_000331"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEP5993830.1"
                     /translation="MNTLVLKIDAILSKHLKKQLAPYTISSQNIYVAFAAKKNGVTVL
                     LYKSGKLVLQGNGANALAQELNLPVAKTVFEASNNSQDIPIIGSDEVGNGSYFGGIAV
                     VASFVDPKDHPFLKKLGVDDSKKLSDKTIQQIAPLLEKQIPHQSLLLSPKKYNELVGK
                     SKPYNTISIKVALHNQAIFLLLQKGIQPKQIVIDAFTSQSNYEKHLKKEKNHFPNPLT
                     FQEKAESHYLAVAVSSIIARNLFLDNLDQLGQDLGYQLPSGAGSASDKVASQLLAAYG
                     MSSLEYSAKLHFANTHKAQALLTK"
     gene            21915..22223
                     /locus_tag="VC394_000332"
     CDS             21915..22223
                     /locus_tag="VC394_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5993831.1"
                     /translation="MKSINRYKFTFGEKTLTLTTDKDNLFMEEVERVAKEKYQALKNH
                     LPEADDETIAILMAINTLSTQLSREIAIEKMEAEILDLRQKTLVGLQEKANQIDVNEV
                     "
     gene            22226..22771
                     /locus_tag="VC394_000333"
     CDS             22226..22771
                     /locus_tag="VC394_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEP5993832.1"
                     /translation="MLSLLIVLVLTWNFYIGYSRGIILQSFYVLGALLSLLVANRFYI
                     GLAHKLTLWIPYSNPVEGTSVFFFKSVDIFVLDKVYYAGLAFFIIFLLGYALSRFLGI
                     FVHFLLLDYFDNQWTKCLSGGLALLVSLLFLNMLLSIFATVPMPFLQHYLHSSFLARL
                     VIEHLPPLTIIIQKLWIQAII"
     gene            22920..25259
                     /locus_tag="VC394_000334"
     CDS             22920..25259
                     /locus_tag="VC394_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEP5993833.1"
                     /translation="MNNKILEQLEFNKVKELLLPYLKTEQSQEELLELEPMTEAPKIE
                     KSFNEISDMEQIFVEHHSFGIVSLSSISESLKRLELSADLNIQELLAIKKVLQSSSDM
                     IHFYSDLDNVSFQSLDRLFENLEQFPNLQGSFQAINDGGFLEHFASPELERIRRQLTN
                     SERRVRQILQDMLKEKAELLSENLIASRSGRSVLPVKNTYRNRISGVVHDISSSGSTV
                     YIEPRAVVTLNEEITQLRADERHEEGRILHAFSDLLRPHVATIRNNAWILGHLDFVRA
                     KYLFMSDNKATIPKISDDSTLALINVRHPLLSNPVANDLHFDHDLTAIVITGPNTGGK
                     TIMLKTLGLAQLMGQSGLPVLADKGSKIAVFNNIFADIGDEQSIEQSLSTFSSHMTHI
                     VSILNEADRNSLVLFDELGAGTDPQEGASLAMAILEHLRLSHIKTMATTHYPELKAYG
                     IETNFVENASMEFDAETLSPTYRFMQGVPGRSNAFEIASRLGLAPFIVKQAKQMTDSD
                     SDVNRIIEQLEAQTLETRRRLDHIKEVEQENLKFNRAVKKLYNEFSHERDKELEKIYQ
                     EAQEIVDMALNESDTILKKLNDKSQLKPHEIIDAKAQIKKLAPQVDLSKNKVLNKAKK
                     IKAARAPRIGDDIIVTSYGQRGTLTSQLKDGRWEAQVGIIKMTLTQDEFSLVRVQEEQ
                     KVKNKQINVVKKADSSGPRARLDLRGKRYEEAMQELDHFIDQALLNNMGQVDIIHGIG
                     TGVIREGVTKYLRRHKHVKHFAYASQNAGGSGATIVTLG"
     gene            25263..25763
                     /locus_tag="VC394_000335"
     CDS             25263..25763
                     /locus_tag="VC394_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEP5993834.1"
                     /translation="MESYEHFYAKLSQPFRKKPQLIILLNVLLKIITGVMYILYPSFL
                     IFTLWQGMTFQLWLRLLIIPAVGFIALSYIRKRFDFPRPYEKWNIKPLIDKDTKGRSM
                     PSRHVFSATMISMCLLRYYVYFGIVCLILSVLLAICRVIAGIHYPKDVIVGYLIGLML
                     GLCLLI"
     gene            25844..26158
                     /gene="trxA"
                     /locus_tag="VC394_000336"
     CDS             25844..26158
                     /gene="trxA"
                     /locus_tag="VC394_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEP5993835.1"
                     /translation="MALEVTDATFVEETKEGLVLIDFWATWCGPCRMQAPILEQLSQE
                     IDEDELKILKMDVDENPETARQFGIMSIPTLMFKKDGEVVKQVAGVHTKDQLKAIIAE
                     LS"
     gene            complement(26210..26798)
                     /locus_tag="VC394_000337"
                     /pseudo
     CDS             complement(26210..26798)
                     /locus_tag="VC394_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402392.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(26874..27998)
                     /gene="mutY"
                     /locus_tag="VC394_000338"
     CDS             complement(26874..27998)
                     /gene="mutY"
                     /locus_tag="VC394_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEP5993836.1"
                     /translation="MWDNETIASFRRTLLGWYDQEKRDLPWRRTTNPYYIWVSEIMLQ
                     QTQVNTVIPYYKRFLEWFPQIKDLADAPEEQLLKAWEGLGYYSRVRNMQKAAQQVMVD
                     FGGIFPHTYDDIASLKGIGPYTAGAIASISFNLPEPAVDGNVMRVMARLFEVNYDIGD
                     PKNRKIFQAIMEILIDPDRPGDFNQALMDLGTDIESAKTPRPDESPIRFFNAAYLNGT
                     YGKYPIKNPKKKPKPMRIQAFVIRNQNGQYLLEKNTKGRLLGGFWSFPIIETSPLSQQ
                     LDLFDDNQSNPIIWQTQNETFEREYQLKPQWTDNHFPNIKHTFSHQKWTIELIEGVVK
                     ATDLPNAPHLKWVAIEDFALYPFATPQKKMLETYLKQKNA"
     gene            28196..28489
                     /locus_tag="VC394_000339"
     CDS             28196..28489
                     /locus_tag="VC394_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5993837.1"
                     /translation="MFQKLKNLIDRYSWFVFIMMILVLFLNYIYPFDPSLSGLQLEWL
                     IMTQVFPGLGIVCAMVAIIFKKFNLFVLALILYFALWLKLFLAFSFFTYFLGV"
     gene            28662..28952
                     /locus_tag="VC394_000340"
     CDS             28662..28952
                     /locus_tag="VC394_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEP5993838.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKDWE
                     KRRLAYEINDFREGLYHIVNLEATDAAALNEFDRLSKINGDILRHMIVKLDA"
     gene            28974..29465
                     /locus_tag="VC394_000341"
     CDS             28974..29465
                     /locus_tag="VC394_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEP5993839.1"
                     /translation="MINNVVLVGRMTKDAELRYTPSQVAVATFTLAVNRTFKSQNGER
                     EADFINCVIWRQPAENLANWAKKGALIGVTGRIQTRNYENQQGQRVYVTEVVADNFQM
                     LESRATREGGSTGSFNGGFNNNTSSSNSYSAPAQQTPNFGRDDSPFGNSNPMDISDDD
                     LPF"
     gene            29630..29869
                     /locus_tag="VC394_000342"
     CDS             29630..29869
                     /locus_tag="VC394_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEP5993840.1"
                     /translation="MAQQRRGGFKRRKKVDFIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPYVNED"
     gene            complement(30002..30658)
                     /locus_tag="VC394_000343"
     CDS             complement(30002..30658)
                     /locus_tag="VC394_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEP5993841.1"
                     /translation="MELQELTKKNQEFIHTATNKLIQDGKSDEDIKLILEEAIPAILE
                     NQKKGVTARNLLGTPTAWAASFSQDPSQKAAETDKNTNPWLMWLDTSLLFIGIVALLN
                     GIMTFFNTNATVTGLISLLALGFGGGASMYATYYFIYRHLGKDKSLRPSWFKIIAALS
                     LAMLIWIALYSATAFLPTSLNPQLPPLALLIIGGISLALRYYLQRKYNIQNTMSPVNK
                     "
     gene            complement(30791..31735)
                     /locus_tag="VC394_000344"
     CDS             complement(30791..31735)
                     /locus_tag="VC394_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEP5993842.1"
                     /translation="MKQMFLSSAIEFKEIETFEPGAWIKLVNPSQEESMKIADQFNID
                     ISDLRAPLDVEETSRIAVEDDYTLIIVDVPIYEERNNKSYYITMPLGIIVTENAVITT
                     CLHDMTLFDHFHNRRVKNFYTFMKTRFVFQILYRNAELFLTALRTIDRQSERLEAQLE
                     AATRNEELIDMMELEKSIVYLKASLKFNERIVKKLSSSTSSLKKYIEDEDLLEDTLIE
                     TQQAIEMAGIYENVLNAMTETTASIINNNQNTIMKTLALMTMALDIPTVIFSAYGMNF
                     QNNWLPLNGLEHAFWYITLIAMLLSSFVVIYFIRKKWF"
     gene            31938..34766
                     /gene="uvrA"
                     /locus_tag="VC394_000345"
     CDS             31938..34766
                     /gene="uvrA"
                     /locus_tag="VC394_000345"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEP5993843.1"
                     /translation="MQNKIIIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGNMEKPDVDSIDGLSPAISIDQKTTSKNPRSTVGTV
                     TEINDYLRLLYARVGTPYCINGHGAITASSAEQIVEQVLALPERTRMQILAPIVRRKK
                     GQHKTIFEKIQKDGYVRVRVDGDIFDVTEVPELSKSKMHNIEVVIDRLVNKDGIRSRL
                     FDSVEAALRLGDGYLMIDTMDGNELLFSEHYSCPVCGFTVPELEPRLFSFNAPFGSCP
                     TCDGLGIKLEVDLDLVVPDPSKSLREGALAPWNPISSNYYPTMLEQAMASFGVDMDTP
                     FEALTEEERDLVLYGSGDREFHFHYVNDFGGERNIDIPFEGVVTNVNRRYHETNSDYT
                     RNVMRGYMNELTCATCHGYRLNDQALCVHVGGEEGPHIGQISELSIADHLQLLEELEL
                     TENESTIAKPIVKEIHDRLTFLNNVGLNYLTLSRAAGTLSGGESQRIRLATQIGSNLS
                     GVIYILDEPSIGLHQRDNDRLIESLKKMRDLGNTLIVVEHDEDTMMQADWLIDVGPGA
                     GEFGGEIIASGTPKQVAKNKKSITGQYLSGKKFIPVPLERRSGNGRFIEIKGAAQNNL
                     QSLDVRFPLGKFIAVTGVSGSGKSTLVNSILKKAVAQKLNRNADKPGKYHSISGIEHI
                     ERLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVPCEVCHGRRYNSETLEVHYKEKNIAEVLDMTVDDALV
                     FFSAIPKIARKIQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSLYIL
                     DEPTTGLHTDDIARLLKVLERFVDDGNTVLVIEHNLDVIKSADHIIDLGPEGGVGGGQ
                     IVATGTPEEVAQVKESYTGHYLKVKLQQ"
     gene            34882..35955
                     /locus_tag="VC394_000346"
     CDS             34882..35955
                     /locus_tag="VC394_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEP5993844.1"
                     /translation="MSGFLEQRLGHCLRQMAEKGLEALLVTHLTNSYYLTGFSGTAAT
                     VLITAKRRVLITDSRYTLLAKASVEGFDIIESRTPLKVVAELLEADQIDCLGFEDQVS
                     FSFYQAMQAELSGITLLAQSGFVEHLRFIKDTSEIDTIAKACSISDKAFEDALDFIKP
                     GTTTERDLANFLDFRMRQYGASGTSFDIIVASGYRSAMPHGRASDKVIQNKESLTMDF
                     GCYYNHYVSDMTRTIHIGQVTDEEREIYALVLAANKALIDKASAGMTYSDFDGIPRQL
                     ITEAGYGSHFTHGIGHGIGLDIHENPFFGKSEQLLQAGMVVTDEPGIYLDNKYGVRIE
                     DDLVITETGCQVLTLAPKELIVL"
     gene            35989..36456
                     /locus_tag="VC394_000347"
     CDS             35989..36456
                     /locus_tag="VC394_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComE"
                     /protein_id="HEP5993845.1"
                     /translation="MTNRLSWQDYFMANAELISKRSTCDRAFVGAVLVKDNRIIATGY
                     NGGVSATDNCNEAGHYMEDGHCIRTVHAEMNALIQCAKEGISTDGTEIYVTHFPCINC
                     TKALLQAGIKKITYKAHYRPHPFAIELMEKKGVAYVQHDVPHIILGGKEGPNG"
     gene            36544..37101
                     /gene="efp"
                     /locus_tag="VC394_000348"
     CDS             36544..37101
                     /gene="efp"
                     /locus_tag="VC394_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEP5993846.1"
                     /translation="MIEASKLKAGMTFESEGKLIRVLEASHHKPGKGNTIMRMKLRDV
                     RTGSTFDTTYRPDEKFEQAIIETVPAQYLYKMDDTAYFMNTDTYDQYEIPVANVEQEL
                     LYILENSDVKIQFYGSEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATLETGLVV
                     NVPDFIEAGQKLIINTAEGTYVSRA"
     gene            37147..37536
                     /locus_tag="VC394_000349"
     CDS             37147..37536
                     /locus_tag="VC394_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEP5993847.1"
                     /translation="MTTEHIGEIVISPRVLEVITGIATTQVEGVHSLHNKKMADSFNK
                     ASLGKGVYLQTEEDGSVTADIYVYLQYGVKVPTVSMNIQKTVKSAVYDMAEVPISAVN
                     IHVEGIVAEKTPKPDLKSLFDEDFLDD"
     gene            37529..37981
                     /gene="nusB"
                     /locus_tag="VC394_000350"
     CDS             37529..37981
                     /gene="nusB"
                     /locus_tag="VC394_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEP5993848.1"
                     /translation="MTNSFQNSRRDLRERAFQALFNIEMGAELLAASQFAYGYDKVTG
                     EDAQVLELPIFLLSLVTGVNNHKEELDNLISTHLKKGWSLERLTLTDKTLLRLGLFEI
                     KYFDETPDRVALNEIIEVAKKYSDETSAKFINGLLSQYVSEAPSANKS"
     gene            complement(38122..39087)
                     /locus_tag="VC394_000351"
     CDS             complement(38122..39087)
                     /locus_tag="VC394_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEP5993849.1"
                     /translation="MVAKLTDVAALAGVSPTTVSRVINQKGYLSQKTVNKVNKAMREL
                     GYKPNNLARSLQGKSTQLIGLIFPNISNIFYAELIEHLEIELFKQGYKTIICNSEHNP
                     VKEREYLEMLAANQVDGIISSSHNLGIEDYERVEAPIVAFDRNLAPNIPVISSDNFEG
                     GKLAAQTLQKHGCQNIVMITGNDNSDSPTGLRQLGFNYQLKRSAEIIKLPNNLSPVRR
                     EMEIKSILATRKPDGLFVSDDLTAILIMKVAKQLHITIPEDMKVIGYDGTTFIQQYVP
                     QLATIRQPIDEIAKLSVEILIKKIKKEKTSKDYILPITLLPGASI"
     gene            complement(39092..40531)
                     /locus_tag="VC394_000352"
     CDS             complement(39092..40531)
                     /locus_tag="VC394_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEP5993850.1"
                     /translation="MDLPQAIRYRPYKEWSSKDYQAITEKMAQSPWHSQFHVEPKTGL
                     LNDPNGFSYFNGHYHLFYQNWPYGAAHGLKQWVHMTSTDLVHFTETRSRLLPDHAHDS
                     HGAYSGSAYAIDDKLFLFYTGNVRDANWVRTPLQVGAWMDKQGNISKIPQVLIEQPDD
                     VTEHFRDPQLFSYQGQFYAIIGAQGLDGKGKIKLYKAVDNHVDNWRFIADLDFDDSGT
                     EYMIECPNLVFVDDKPVLIFSPQGLAKADLDYQNIYPNTYKIFESFNPETGQLLGGGA
                     LQNLDFGFEAYATQAFSSPDGRVLAVSWIGLPDIDYPTDRYDYQGALSLVKELRIKDG
                     ILYQTPVSALQNLRGPAELFHNKIDSSNCYELELTIPGQKKLDLLLFADQKGNGLRLK
                     VDTTKGQLSIDRSRAGVQYAQDYGTVRSCQIPQGHVTLNVYVDNSILEIFINQGQKVL
                     TSRVFPTQGQTGIQVVEGQAFGHYYEMRY"
     gene            40773..42656
                     /locus_tag="VC394_000353"
     CDS             40773..42656
                     /locus_tag="VC394_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEP5993851.1"
                     /translation="MDNRQIATEVIEALGGRENVRSVAHCATRLRVMVHDEGKIDKEK
                     AEAIDKVKGAFFNSGQYQMIFGTGTVNNIYDEVVALGLPTSSTSEQKAEAGKQGNIFQ
                     RAIRTFGDVFVPIIPAIVATGLFMGVRGLVTQPAIMDLFGVHEYGENFLMYTRILTDT
                     AFVYLPALVAWSAFRVFGGNPIIGIVLGLMLVSNELPNAWVVASGGDVKPLTFFGFVP
                     VVGYQGTVLPAFFVGLVGAKLEKWLHKKVPEALDLLVTPFLTFAIMSTLGLFVIGPVF
                     HSLENLVLAGTQAVLHLPFGIAGLIVGGIQQLIVVTGIHHIFNFLEAQLIANTGKDPF
                     NAYLTAATAAQAGATLAVAVKTKSTKLKGLAFPSTLSALLGITEPAIFGVNLRYPKVF
                     VSGLIGGALGGWVAGLFGIAGTGFGITVLPGTLLYLNGHLLQYLVTMLVGLGVAFGIA
                     YTWGYQDRETLPLPAVEVDQTADQPALAEETLYSPLNGTVVDLAAVSDPVFSSGAMGQ
                     GLAIKPEDNTLYSPVDGKVEIVFETGHAYAITSSQGAEVLLHIGIDTVSMAGDGFESL
                     VAVGQAVKKGDLLGHFDPSKIAEAGLDDTTMMIVTNSADYQSVDILAQGHVLIGDQVA
                     LIK"
     gene            42887..45901
                     /locus_tag="VC394_000354"
     CDS             42887..45901
                     /locus_tag="VC394_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-beta-N-acetylglucosaminidase"
                     /protein_id="HEP5993852.1"
                     /translation="MDKHLLVKRTLGCVCAATLMGAALATHHDSLNTVKAEEKTVQVQ
                     KELSSIDSLHYLSENSKKEFKEELSKAGQESQKVKEILAKAQQADKQAQELAKMKIPE
                     KIPMKPLHGPLYGGYFRTWHDKTSDPTEKDKVNSMGELPKEVDLAFIFHDWTKDYSLF
                     WKELATKHVPKLNKQGTRVIRTIPWRFLAGGDNSGIAEDTSKYPNTPEGNKALAKAIV
                     DEYVYKYNLDGLDVDVEHDSIPKVNGEASDENLKRSIDVFEEIGKLIGPKGADKSRLF
                     IMDSTYMADKNPLIERGAPYIDLLLVQVYGARGEQGEFQNDTKLVTDTPEERWQGYSK
                     YIRPEQYMIGFSFYEERAGSGNLWYDINSRKDEDKANGINTDITGTRAERYARWQPKT
                     GGVKGGIFSYAVDRDGVAHQPKQIAEKDKQNVKNNQPQIPEITDNIFHSDYSVSKALK
                     TVMLKDKSYDLIDEKDFPDKALREAVMAQVGTRKGDLERFNGILRLDNPAIQSLEGLN
                     KFKKLAQLDLIGLSRITKLDQSVLPANMKPGKDTLETVLETYKKDNKEEPATIPPVSL
                     KVSGLTGLKELDLSGFDRETLAGLDVATLTSLEKVDISGNKLDLAPGTENRQIFDTML
                     STVSNHVGSNEQTVKFDKQKPTGHYPDTYGKTSLRLPVANGKVDLQSQLLFGTVTNQG
                     TLINSEADYKAYQNQQIAGRSFVDSNYHYNNFKVSYENYTVKVTDSTLGTTTDKTLAT
                     DKEETYKVDFFSPADKTKAVHTAKVIVGDEKTMMVNLAEGATVIGGSADPVNARKVFD
                     GQLGSETDNISLGWDSKQSIIFKLKEDGLIKYWRFFNDSARNPKTTNKPIQEASLQIF
                     NIKDYNLDNLLENPNKFDDEKYWITVDTYSAQGERATAFSNTLNNITSKYWRVVFDTK
                     GDRYSSPVVPELQILGYPLPNADTIMKTVTIAKGLSQQKDKFSQKMLDELKIKEMALE
                     TSLNSKIFDVTAINANAGVLKDCIEKRQLLKK"
     gene            46165..47049
                     /locus_tag="VC394_000355"
     CDS             46165..47049
                     /locus_tag="VC394_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEP5993853.1"
                     /translation="MGKLYGSIEAGGTKFVCAVGDEEFTVVDKTQFLTTTPEETIART
                     IAYFKAFEADLAGMAIGSFGPIDIDPSSETYGHITTTPKSGWANVDLLGQLSAAFKIP
                     FDVTTDVNSSAYGEVLARPGVESLVYYTIGTGIGAGAIQHGHFIGGLGHTEAGHTYVM
                     PHPDDMAKGFLGVCPFHKGCLEGMAAGPSIEARTGVRGERLDQEADVWDIQAFYIAQA
                     ALQATMLYRPQVIVFGGGVMTQEHMVLRVHDKFTTLLSGYLPVPDLTDYIVTPAVADN
                     GSATLGNFALAKLAAQGK"
     gene            47144..48097
                     /gene="manA"
                     /locus_tag="VC394_000356"
     CDS             47144..48097
                     /gene="manA"
                     /locus_tag="VC394_000356"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEP5993854.1"
                     /translation="MSEPLFLKSTMHDRIWGGTKLRDVFAYNIPSDTTGEYWAISAHP
                     NGVSTVTNGRYQGQPLNTLYAQEPALFGNPKEEVFPLLTKILDANDWLSVQVHPDDAY
                     GREHEGELGKTECWYIISAEEGSEIVYGHQAKSKEDLRAMIEAGAWDDLLTRVPVKAG
                     DFFYVPSGTMHAIGKGILILETQQSSDTTYRVYDFDRKDVNGNLRDLHIEKSIDVLTI
                     GKPENSVPATIVLDNMVETTLVSTPFFTVYKWVTSQMVDMKQAAPYLLVSVLKGQGKL
                     YVDQKAYELEKGMHFILPNDVKSWSFDGQLEMIVSHPNQLV"
     gene            48231..50750
                     /gene="secA"
                     /locus_tag="VC394_000357"
     CDS             48231..50750
                     /gene="secA"
                     /locus_tag="VC394_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEP5993855.1"
                     /translation="MANILRKVIENDKGELRKLEKIAKKVESYADQMVSLSDRDLQGK
                     TLEFKERYQKGETLEQLLPEAFAVVREAAKRVLGLFPYRVQIMGGIVLHNGDVPEMRT
                     GEGKTLTATMPVYLNAIAGEGVHVITVNEYLSTRDATEMGEVYSWLGLSVGINLAAKS
                     PAEKREAYNCDITYSTNSEVGFDYLRDNMVVRQEDMVQRPLNFALVDEVDSVLIDEAR
                     TPLIVSGAVSSETNQLYIRADMFVKTLTSVDYVIDVPTKTIGLSDSGIDKAESYFNLS
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEDGEILIVDQFTGRTMEGRRFSDGL
                     HQAIEAKEGVRIQEESKTSASITYQNMFRMYKKLAGMTGTAKTEEEEFREVYNMRIIP
                     IPTNRPIARIDHTDLLYPTLESKFRAVVEDVKTRHAKGQPILVGTVAVETSDLISRKL
                     VEAGIPHEVLNAKNHFKEAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMRRFGSDRIKAFLDRMKLDE
                     EDTVIKSGMLGRQVESAQKRVEGNNYDTRKQVLQYDDVMREQREIIYANRRDVITANR
                     DLGPEIKAMIKRTIDRAVDAHARSNRKDAIDAIVTFARTSLVPEESISAKELRGLKDD
                     QIKEKLYQRALAIYDQQLSKLRDQEAIIEFQKVLILMIVDNKWTEHIDALDQLRNAVG
                     LRGYAQNNPVVEYQAEGFKMFQDMIGAIEFDVTRTMMKAQIHEQERERASQRATTAAP
                     QNIQSQQSANTDDLPKVERNEACPCGSGKKFKNCHGRKSFS"
     gene            50866..51222
                     /locus_tag="VC394_000358"
     CDS             50866..51222
                     /locus_tag="VC394_000358"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEP5993856.1"
                     /translation="MIVGHGIDLQEISAIEKVYQRNPRFAQKILTEQELAIFESFPYK
                     RRLSYLAGRWAGKEAFAKAIGTGIGRLTFQDIEILNDVRGCPILTKSPFKGNSFISIS
                     HSGNYVQASVILEDKK"
     gene            51219..52319
                     /locus_tag="VC394_000359"
     CDS             51219..52319
                     /locus_tag="VC394_000359"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEP5993857.1"
                     /translation="MISSFHRPTVARVNLQAIKENVASVQKHIPLGVKTYAVVKADAY
                     GHGAVQVSKALLPQVDGYCVSNLDEALQLRQAGIDKEILILGVLLPNELELAVANAIT
                     VTIASLDWIALARLEKKECQGLKVHVKVDSGMGRIGLRSSKAVNLLIDSLKELGADVE
                     GIFTHFATADEADDTKFNQQLQFFKKLIAGLEDKPRLVHASNSATSIWHSDTIFNAVR
                     LGIVSYGLNPSGSDLSLPFPLQEALSLESSLVHVKMISAGDTVGYGATYTAKKSEYVG
                     TVPIGYADGWTRNMQGFSVLVDGQFCEIIGRVSMDQLTIRLPKAYPLGTKVTLIGSNQ
                     QKNISTTDIANYRNTINYEVLCLLSDRIPRIY"
     gene            52406..53917
                     /locus_tag="VC394_000360"
     CDS             52406..53917
                     /locus_tag="VC394_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEP5993858.1"
                     /translation="MNKNKLLRVAMLLSLLAPTAESMTVLAQDVMLETHKATTNETSD
                     SSSKEENNKNAAPTTSDKTDQGPLDASAETNSNSLVNADDKKRSDSSQSAVGSSNNKA
                     EAENQVDDKSTDHSKPTDQPKPTDQPKPSPSKVDTAPASSLSRQLPEARTPIQSLSPY
                     VSDLDLSEIDIPSVNTYAAYVEHWSGKNAYTHHLLSRRYGIKADQIDSYLKSTGIAYD
                     STRINGEKLLQWEKKSGLDVRAIVAIAMSESSLGTQGIATLLGANMFGYAAFDLDPTQ
                     ASKFNDDSAIVKMTQDTIIKNKNSNFALQDLKAAKFSRGQLNFASDGGVYFTDTTGSG
                     KRRAQIMEDLDKWIDDHGGTPAIPAELKVQSSASFASVPAGYKLSKSYDVLGYQASSY
                     AWGQCTWYVYNRAKELGYQFDPFMGNGGDWKYKAGYALSKTPKVGYAISFAPGQAGAD
                     GTYGHVSIVEDVRKDGSILISESNCIGLGKISYRTFTAQQAEQLTYVIGKSKN"
     gene            54407..58234
                     /gene="shr"
                     /locus_tag="VC394_000361"
     CDS             54407..58234
                     /gene="shr"
                     /locus_tag="VC394_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein Shr"
                     /protein_id="HEP5993859.1"
                     /translation="MKKISKCAFVAISALVLIQATQTVKSQEPLVQSQLVTTVALTQD
                     NRLLVEEIGPYASQSAGKEYYKHIEKVIVDNDVYEKSLEGERTFDINYQGIKINANLI
                     KDGKHELTIVNKKDGDILITFIKKGDKVTFISAQKLGTTDHQDSLKKDVLSDKTVPQN
                     QGTQKVVKSGKNTANLSLITKLSQEDGAILFPEIDRYSDNKQIKALTQQITKVTVNGT
                     VYKDLISDSVKDTNGWVSNMTGLHLGTKAFKDGENTIVISSKGFEDVTITVTKKDGQI
                     HFVSAKQKQHVTAEDRQSTKLDVTTLKKAIKEADAIIAKESNKDAVKDLAEKLQVIKD
                     SYKEIKDSKLLADTHRLLKDTIESYQAGEVSINNLTEGTYTLNFKANKENSEESSMLQ
                     GAFDKRAKLVVKADGTMEISMLNTALGQFLIDFSIESKGTYPAAVRKQVGQKDINGSY
                     IRSEFTMPIDDLDKLHKGAVLVSAMGGQESDLNHYDKYTKLDMTFSKTVTKGWSGYQV
                     ETDDKEKGVGTERLEKVLVKLGKDLDGDGKLSKTELEQIRGELRLDHYELTDISLLKH
                     AKNITELHLDGNQITEIPKELFSQMKQLRFLNLRSNHLTYLDKDTFKSNAQLRELYLS
                     SNFIHSLEGGLFQSLHHLEQLDLSKNRIGRLCDNPFEGLSRLTSLGFAENSLEEIPEK
                     ALEPLTSLNFIDLSQNNLALLPKTIEKLRALSTIVASRNHITRIDNISFKNLPKLSVL
                     DLSTNEISNLPNGIFKQNNQLTKLDFFNNLLTQVEESVFPDVETLNLDVKFNQIKSVS
                     PKVRALIGQHKLTPQKHIAKLEASLDGEKIKYHQAFSLLDLYYWEQKTNSAIDKELVS
                     IEEYQQLLQEKGSDTVSLLNDMQVDWSIVIQLQKKASNGQYVTVDEKLLSNDPKDDLT
                     GEFSLKDPGTYRIRKALITKKFATQKEHIYLTSNDILVAKGPHSHQKDLVEKGLRALN
                     QKQLRDGIYYLNASMLKTDLASESMSNKAINHRVTLVVKKGVSYLEVEFRGIKVGKML
                     GYLGELSYFVDGYQRDLAGKPVGRTKKAEVVSYFTDVTGLPLADRYGKNYPKVLRMKL
                     IEQAKKDGLVPLQVFVPIMDAISKGSGLQTVFMRLDWASLTTEKAKVVKETNNPQENS
                     HLTSTDQLKGPQNRQQEKTPTSPPSAATGIANLTDLLAKKATGQSTQETSKTDDTDKA
                     EKLKQLVRDHQTSIEGKTAKDTKTKKSDKKHRSNQQSNGEESSSRYHLIAGLSSFMIV
                     ALGFIIGRKTLFK"
     gene            58431..59306
                     /locus_tag="VC394_000362"
     CDS             58431..59306
                     /locus_tag="VC394_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEP5993860.1"
                     /translation="MTKVVIKQLLQVIVVFMISLSTMTGVVYADKGQIYGCIIQRNYR
                     HPISGQIEDSGGEHSFDIGQGMVEGTVYSDAMLEVSDAGKIVLTFRMSLADYSGNYQF
                     WIQPGGTGSFQAVDYNITQKGTDTNGTTLDIAISLPTVNSIIRGSMFVEPMGREVVFY
                     LSASELIQKYSGNMLAQLVTETDNSQNQEVKDNQKPVDTKLGESQDESHTGAMITQNK
                     PKANSSNNKSLSDKKILPSKMGLTTSLELKKEDKFRSKKDLSIMIYYFPTFFLMLGGF
                     AVWVWKKRKKNDKTM"
     gene            59290..60174
                     /gene="isdE"
                     /locus_tag="VC394_000363"
     CDS             59290..60174
                     /gene="isdE"
                     /locus_tag="VC394_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     IsdE"
                     /protein_id="HEP5993861.1"
                     /translation="MIKRCKGIGLALMAFFLVACVNQHHKTAKESEQQRIVATSVAVV
                     DICDRLNLDLVGVCDSKLYTLPKRYDAVKRVGLPMNPDIELIASLKPTWILSPNSLQE
                     DLEPKYQKLDTEYGFLNLRSVEGMYQSIDDLGNLFQRQQEAKELRQQYQDYYRAFQAK
                     RKGKKKPKVLILMGLPGSYLVATNQSYVGNLLDLAGGENVYQSDEKEFLSANPEDMLA
                     KEPDLILRTAHAIPDKVKVMFDKEFAENDIWKHFTAVKEGKVYDLDNTLFGMSAKLNY
                     PEALDTLTQLFDHVGDHS"
     gene            60176..61198
                     /locus_tag="VC394_000364"
     CDS             60176..61198
                     /locus_tag="VC394_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEP5993862.1"
                     /translation="MTASDLQRDQSLLAKKRFRSGLYLVLLILSLLFLAVIALSLGGL
                     AVSYGAIVKGLFVAYDPQVALIYDLRFPRIVIALLAGAGIAVSGVLFQAVLKNPISDP
                     AIIGICSGASFMVLVSSLLLPQLLLYGPIVSFLGGGVSFLLIYGLAWKKGLNPIRLIL
                     TGIAINALFMGLSTALTSFFTSASPMVNALLAGHISQKTWADVGVLFPYTFIGLLLAL
                     LLSKTCNLLLLDDQVIRHLGIDATALRLGISLVAVLLASVATSIVGVVSFLGLIVPHM
                     SRLLVGSKHQILIPFSALLGAFVFLLADTLGRSLAYPLEISPAIIMSIVGGPYFIYLL
                     RRSDII"
     gene            61195..62031
                     /locus_tag="VC394_000365"
     CDS             61195..62031
                     /locus_tag="VC394_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5993863.1"
                     /translation="MSKNSSHIQVQNLSFAYEEKLVLDDLTFSIPKGKITTIMGANGS
                     GKSTLLQLLTKNLPLKQGQVWLEQEELATISLKAFAKKVAVVHQYHQVVEGLLVGELV
                     AMARLARRSFFKTTTKEDEERLAWALEVTALSDYVYRDLQHLSGGEQQRVWIAMALAQ
                     DTDIIFLDEPTTYLDIKYQLDILKLITKINQELGLTIVLVLHDINQALSLSDHLIGLK
                     DGRLYAQGQPRDLLDQQFIRDVFGVELPFVERNGQQFVLTSLVNETNDIRTQRRDRNK
                     NR"
     gene            62028..63791
                     /locus_tag="VC394_000366"
     CDS             62028..63791
                     /locus_tag="VC394_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEP5993864.1"
                     /translation="MMETEDTISRGKRKRLLKRLRERIAPKRYLLYVSAFLSWLQFVM
                     RMISFYLIAKTFSTFILGHAIALGRLAGLLLLLNVVGFVLAILGKQLQGIASQFARDS
                     LKQSFFEAFIDLDGQFDAHASDADILTLASQGIDSLDTYYGYYLSLSMRTKWNCTTIM
                     ILVFLIYPLAGLVFLGVLLLIPLSIVAMQKRSKQIMSHYWSSYMDVGNLFMDDLKGLN
                     TLYSYQAIERYEQEFSGKAEQFRKATMSLLGFQLQAVGYMDAVMYLGIGLSGFLAVQA
                     LATGQLSFFNFLFFLLIATEFFTPIREQGYGMHLVMMNTKMADRIFSFLDSVPARKDN
                     KSKTAINFNQIDIQNISLAYEKKTVLSGVTMTLTKGQLTAIAGVSGQGKTSLAQLLLK
                     RQSATTGHILFDGLDSDNLLQETINQQVLYVSDQSTLLNRSIYDNLRLAANLSKKEIL
                     DWIDQHGLLSFINWLPDGLDTIVGENGNLLSPGQKQQVICARALLSKRSLYIFDEATS
                     SLDAENERIIDNLITRLAKTAIVIVITHKMSRLKGANQVLFLNTGQPACLGKPCDLYR
                     DQPTYRHLVDTQARLEASIYG"
     gene            63784..65454
                     /locus_tag="VC394_000367"
     CDS             63784..65454
                     /locus_tag="VC394_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5993865.1"
                     /translation="MAKPNRSTLSLVWRLLTLMKTLLPWIILAVGFAVMGFVITVSIP
                     TGIAYLGLLAIRQEVIPILALYLLIALAFLRGFVRYGEHYFGHFVAFHSLAAFRNLIF
                     KKLRALSPACLDSQDSGYLLKMIGEDIEALEVFFAHTIAPICTAILSAGLMFWYFCQS
                     SWQLALLALATYACLAIVIPIYFANILQVLLKSQNEGRKDYLSYFLESLRSVKDLLQF
                     QVLDEQFERLIKKSNHVNALDRNVAQAQFLQMALTFFWLGLMILAFSYMVFDGICHDS
                     LSFDKGLLTFIAFTASFSPFLELGRLPLGFKRAMNAARNIFDLLDENVIVDEGTKQID
                     RLRSVSFEDLSFAYPKRQELVFKDLSVTFQEKGIIGIKGESGSGKSTLVKLIMKWYNW
                     KTGDIFLNDRNSCLLNAAKLQDTIAYVPQTAQLFQQSIRENLTFGRQDISDESIWNLA
                     ETCGMKDRLLACKEGLDTIIKSPSDFSAGEGQRLELMRALLKDASCYIFDEPTSNLDS
                     LNEAILLDLIKTHCQGLVFLISHRPSTLACADHLFCVKNGSLKEVNKK"
     gene            65451..66044
                     /locus_tag="VC394_000368"
     CDS             65451..66044
                     /locus_tag="VC394_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEP5993866.1"
                     /translation="MKQLTIKDIMITGAFAALYFICVGLGTFINIFFDRSGNMLYAPA
                     FAALLGGTIYFLLIAKLQTFGPITLLGLMMGGFFFLSGHFFAAMFPSIIFGLIADIIA
                     SLGKYKSRFFNDLSYMVFAFVNSGPIVLMWLLKDAYVESLLARGKSMTYVNRIMYDFS
                     LGHVIWFVGIVLAGALLGSAFAHYLLKKHFIKSGLLS"
     gene            66041..66721
                     /locus_tag="VC394_000369"
     CDS             66041..66721
                     /locus_tag="VC394_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEP5993867.1"
                     /translation="MRLDVRTKLLLLVLANACFFFRVDGFLEFIIVIFLLLLLSALNK
                     KKLAFKLAVVYLLMIGLSVIPLSIFPSYLDHLLSFVSIAGRLVFPSLLAGLITIKTTT
                     IYELVHGLRKWHFPEVWLLTLAVMCRFIPMIRQECCVIHRSLTIRGIILTKWSILIRP
                     KQYLEYLMVPLLLSLIRSSQELTIASLTKGLAVNKGTSECFSSHLTWKDWGVQIWITV
                     IIIITILQ"
     gene            66658..68112
                     /locus_tag="VC394_000370"
     CDS             66658..68112
                     /locus_tag="VC394_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5993868.1"
                     /translation="MERLGGADLDYCYHNHHDTAMISAEQLVFTYHDQKNPACQVSTC
                     QIASGQFIVLCGPSGSGKSTFLKLLNGIIPDYYAGKYEGRLDVADCQADRDSVETFSR
                     SVASVFQNPASQFFYREVQHELVFPCENQGLDAKVIMKRLWTLAEDFAFAELLNKDMF
                     GLSGGQKQRVAIATAIMQGTNIMLFDEPTANLDSAGIAAVKAYLTQLKAAGKTIIVAE
                     HRLHYLMDLADNFFYFKNGRLTDKLTTQDLLALTDEQRQDMGLRRLDLSDLKPVLAGK
                     IESQHYRPDDSLCIEHLTVRAGSKILRCIEQLSFAAGSISGITGSNGLGKSQLVYYIA
                     GILDDKKATIKFQGIPLSAKQRLSKTSIVLQEVSLQLFAESVSKEVNLGHERHPRTTE
                     VIERLSLTTLLERHPASLSGGEQQRVMIAASLLADKDILIFDEPSSGLDLLQMKALAN
                     LLMQLKTQHKVVILISHDEELLSSVCDTIYQMLP"
     gene            68487..70502
                     /gene="recG"
                     /locus_tag="VC394_000371"
     CDS             68487..70502
                     /gene="recG"
                     /locus_tag="VC394_000371"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEP5993869.1"
                     /translation="MILTAPMSNLKGFGPKSAEKFQKLDIYTVEDLLLYYPFRYEDFK
                     SKSVFDLVDGEKAVITGLVVTPANVQYYGFKRNRLSFKLRQGEAVLNVSFFNQPYLAD
                     KIELGQEVAVFGKWDATKSAITGMKVLAQVEDDMQPVYRVAQGISQSTLIKAIKSAFE
                     IDAHLELKENLPATLLEKYRLMGRSQACLAMHFPKDITEYKQALRRIKFEELFYFQMN
                     LQVLKAENRSETNGLPILYSKHAMETKISSLPFILTNAQKRSLDDILSDMSSGAHMNR
                     LLQGDVGSGKTVIAGLSMYAAYTAGFQSALMVPTEILAEQHYISLQELFPDLSIAILT
                     SGMKAAVKRTVLAAIANGSVDMIVGTHALIQDSVQYHKLGLVITDEQHRFGVKQRRIF
                     REKGENPDVLMMTATPIPRTLAITAFGEMDVSIIDELPAGRKPIMTRWVKHEQLGTVL
                     EWVKGELQKDAQVYVISPLIEESEALDLKNAVALHAELSTYFEGIAKVALVHGRMKND
                     EKDAIMQDFKDKKSHILVSTTVIEVGVNVPNATIMIIMDADRFGLSQLHQLRGRVGRG
                     YKQSYAVLVANPKTDSGKKRMTIMTETTDGFVLAESDLKMRGSGEIFGTRQSGIPEFQ
                     VADIVEDYPILEEARKVSAAIVSDPNWIYEKQWQLVAQNIRKKEVYD"
BASE COUNT        21769 a        12334 c        14755 g        21781 t
ORIGIN      
        1 tgaaactcat caataaagac aaaaaaaata tctgctgtac ttgaaaaagt agggctctct
       61 agtggctatt taaatcaaaa aatatatgaa ttgtctggag ggcaggctca gcgtgtagct
      121 attgcaagaa tgttaatgaa gccacgtaaa ataattttag cagatgagcc aacaggagct
      181 ttggatggtg aaattggaaa agaaattatt aatttgttgt tgagcgaaac agccgaaaat
      241 aaatacgtta ttatagcaac tcatgatcca gctgtatata ataaggttga ttttataatt
      301 gatatgaaag atatagggag taagaaatga aaaagaaaat ttatattggc ttgattgtca
      361 ttgttggagt ttttgtagtg ttcttgtctg gaaaacaaat cacacaaaaa gaaacttcaa
      421 ataagcctgt ggtgacccta acagtttata ccatctcctc tagtgatacc caaaaatgga
      481 gtaaagtaaa gcagtttgaa acgaaagaag caatatacct agtaaaggta aaagaaacta
      541 cctcttcaga agaagtcttt tcaaacatca ttgcagatgg aatggcagtt ggttttggag
      601 ttagtgaaga agacgttaaa cagtttaacg ataatttatg tagcccggta gaagatgcta
      661 agaataataa attaattgga atagaattca tcaatttttc tagtgttgat aaggggtttg
      721 tggttgcaaa ttttgactac ggcaaaaaag cgtttaattc tcagaaaaca gaaaataaaa
      781 aattatataa aaagctctat gaagcgtata aaggatagct atttatagtt ttttgagagt
      841 gataccactt ctgaataata aatgaactaa catggcatac cgttttctat tcatctaaca
      901 atgttttcaa gaaataattg atgcctttag cacgactgaa atgaggtcaa attgacagaa
      961 tactcctatt atggtactat ttttaataag aatattcagt gatagtgata aagaggaaaa
     1021 tatgggaatt gaaaagacag ttagtgaact agctgatatt ttgggagtga gtcgtcaggc
     1081 cgtcaataat cgtgttaagt ctttacccga agaagatctt gacaaaaatg aaaaaggcgt
     1141 tactgtggtt aagcgcagtg gtcttgttaa gctggaagag atctataaaa aaacaatttt
     1201 tgacgatgag cctatcagtg aagaaacgaa acaacgtgag cttttagaga ttcttgtgga
     1261 tgagaagaac actgaaatca cgcgccttta tgagcaactc aaagccaaag atgctcaact
     1321 tgcctcaaaa gatgagcaaa tgcgtgttaa agacgtgcag atcgctgaaa aagataaaca
     1381 attggatcag cagcaacaat taactgctaa agccatggct gataaagaaa ccctaaaact
     1441 agaattggaa gaagctaaag cagaagccaa ccaagctcgc ttacaagtcg aagaagttca
     1501 ggctgaagtt ggccctaaaa aaggcttttt aacccgcttg tttgcgaaat aatcacaaaa
     1561 aatgacgaag gccctatcag ggtctttttt ggaggaaaac atgaaacact tagatactta
     1621 tattgccttt gatttagaat ttaacactgt caatgacgtt agtcatgtca ttcaagtatc
     1681 agcagtcaaa tatgaccacc ataaagaagt tgacagcttt gacacctatg tttacacaga
     1741 tgttccctta cagagtttta tcaatggcct aacaggaatc acatctgaca agattgctgc
     1801 ggcaccaaaa gtagaggagg ttatggctgc gtttaaaaat tttgtgggag agttaccact
     1861 gattggttat aatgctcaaa aatctgacct gcctattcta gctgaaaatg gcttagattt
     1921 aagggaccaa taccagattg acctctttga tgaagcttac gatagacgta gcgcagactt
     1981 aaatgggatt gctaaccttc gtctgcaaac agtggcaacc tttttaggca ttaagggtcg
     2041 aggtcataat agcttagaag atgccagaat gacagcagca atctataagt catttttaga
     2101 aacagataca aacaaagcat atctcagcca gcaagaagag gtaaccacag ataacccttt
     2161 cgcagctttg ggagactttt ttgactaagg gctctgctat ttctaaaaaa taacaaaaat
     2221 tcctagggtt agctcttgct tgtaacgcta ggtcatgtga taagataggg gcatatcaag
     2281 agggagtcga gtgcacacga atcgtttttg atcactaaat gatagaaaga aggtgtggaa
     2341 atgtctcaat tctattcgac tggagaattg gccaacttag ctggcgtttc tattcgaact
     2401 gtccaatact atgaccaacg tgggattttg attccgacag ctttgacaga aggtggacgg
     2461 cgactctata cggattctga tttagaacaa ttacgcatga tttgctttct gcgcgatttg
     2521 ggattttcca ttgagcagat tcgcaaagtc ttagcagagg aaaatgcagc ccaggtgctg
     2581 gaattgcttc tagttgatca tattgcgact gccaaggaag acttggccgc aaaagagcaa
     2641 caagttgata tagctgtcaa gatacttgac agacttcgaa aacaagaccc ccagtcacta
     2701 gattttctga tggacatttc gcttagcatg aaaaatcaaa aagcttggaa aaaacgcttt
     2761 attactttaa ttgtctgttt gacagtggaa actctacttt ttgtgggtgg agcctatttt
     2821 ctccaattga cagtctggaa atatttatta atcccggtgt tccttttagt cttactagga
     2881 accttatact ggtattacca aaatatctgt tatctttgtc cttcttgtca tcaaacattt
     2941 gaagcaaaat ggacagaatt tgcccttgca ggccatacac cacgaaccag aaagttaacc
     3001 tgctcccatt gccatcaaaa atcgtattgc ttggaactgg caaaagagga cactgtctca
     3061 agaggataag cactaactca taaaaaaact agcctgagat ccttctcaag ctagtttttg
     3121 tttttttaga atggtaattt accagcaaaa gcgcctaatg ttttcttagt cgcttcatca
     3181 atttgcgtaa gggcatcatt aatggcttga gtggtcatgt cttgaagcgt ttctacatct
     3241 tcaggatcta caacagcttc tttgaagtca atcccaacca attttttatc acccgtgaaa
     3301 gtggcagtga caaggtcttg agctgatttt ccagtaaatt gcatagcagc caaatccgcc
     3361 tgcttttgct ccatttgttt ctgaagcttt tgtgcttgct tcatcatgtt ttgcatgttc
     3421 atcataagac tatactctcc ttaatttaat actaactatt ataacatata aaaagaggca
     3481 ataaaaacaa tgtcagttag tagactagcc tgtatcagta agaaagtcgc tatggataag
     3541 atggattagc agcaagggtc tggctaatga cagaataagt ctagaaatgg attatttttg
     3601 tcatagacta gtccgactta gtggttatct aattcacgat cttctttagc ttgttgttct
     3661 aaaagaattt gtgcgaagtc aacccactta gtgtagttat tttcggataa ttgttctgaa
     3721 atcatcgcta actctcgttg tggcaactga taagagactc ccttactttc taaggttaaa
     3781 tcttcttcca ttagggcggt taaactaaca tcaaagatag cagcaatatc acttaataga
     3841 ccagactttg gagtatattt tcctttttcc cactcactga ttgaagaaga gctttttcgt
     3901 cctaggagat tagcaagttc taattgttcc atgttctttt ttaatctaag gtactttaaa
     3961 ttacttgcaa aaaaagtttt cttggcggac atatttttct ccttttcttt atataaaata
     4021 tgataccata aaagaggtta aagcaaaagg tatcgtaaaa gatggtctaa aaactctcta
     4081 aaaacttgac ttcataaaaa ccgaagttta taataaactt aacatgatta actaatctca
     4141 ttttagtgtg gagggaaaat tgtgaccaaa tcaactttaa aagagttaag aacaacaaag
     4201 aaaatgacac agcaggaatt gtctcactta acaggcattt ctgtcagaac gattgctagg
     4261 tatgaaaaag atactaaaaa gctaaggcga gcaaaatatg agaagctaaa aggtattgca
     4321 caagcattag cggtttccgt tgatgatatt tttttaggaa tagattcgga atttatgaac
     4381 taagacgatc gttttctgtt tgctattagt aagaccaatt aacaaaaaag tgcttagtta
     4441 tattgctaag cacttttatt tgttaatttg atgctattgg cacaatcaat tgagactgac
     4501 tgagatcgat agttaaagca tagttgctgt tgtcacggac agtatgttca aaatcggttg
     4561 tgtataagat gactcgaagg gtatctcctt tttctagatg atagatagtt ggttgcaaag
     4621 gaagtctaac agtcatccat ttattattag gaatagtttc aattgacgac aaattattgc
     4681 gattttgtaa attcatgaaa cctttcgaaa taacacggta agaatcttct ctaaatggta
     4741 gctctttaag gggttctcta gagaaatttt gcccattgtc aatacttgat tgggtgagag
     4801 caataggaag atcgcccaag cgttttttct taccatagtc aaggatttgt gcagatagaa
     4861 gtcctttatt ttcacttgat ttcactttta actgtaggac aatttcaccg ttaataggta
     4921 agtcctcttc cagtaaaata tcaatcaggg cttgattggc cttgccttta aagagagctg
     4981 ctttggacgc acggaaatct ttaccgtaag ctttaaattc atcttcacca tagtgattat
     5041 caattaaaag cagttcctga ccgaggtcaa gctctttgat ggttgaggtt ccaaagactt
     5101 tacgctcctg ccagttttgt gggcaggtgt tatcctgcca gatcatttca ggaaggctaa
     5161 aatcattagc taggcctaag agtttttgac ataaaagggc attcatgctt tcgcgaaaat
     5221 caatggattg ccaattatgc atgtaaacat gctcaccttg atgtaaaaag aggtgcttgt
     5281 tgattgtgga aggaagcgca ttgaaaatct catagacctg tcttggtttg acattccagt
     5341 cttgtaagcc atgactataa acgacatcac actttatttg atgagcattt tttaagtaat
     5401 tgcggtcgtg ccaaaattga ttataatcac cagattgtcg gtctaacgcc tgagactgtt
     5461 ggtttagcaa ttcttgataa tgatcattat ggcgaagata atcgccagcc aataaattac
     5521 gcgagtaggt gagctcagtt aggacatcta gatcctctcc aggatagcca ccaggactac
     5581 aaactaagcc attttcgcgg tagtagttgt accaagaaga aatagctgat tctgcaataa
     5641 tcatggctaa tccgtctacg cctgtagtgg ctaaaccagt agacatagtt cccaagtagg
     5701 actttccagt cgtacaaact agaccatttg cccaatcagc tctcacttgg tgggtttttg
     5761 agtggctggt gtaggctgtg gctcttccat ttagccaatc aataacggct ttaaagcttt
     5821 caatttgagc atagtctcca ctcgtcataa agccagtgga accagcagtt cccacacctg
     5881 aaacatagat attagcaaac ccacgtgcga ggaaataatc gttgaggcta taggagttga
     5941 tgtagctaaa gctttctaga ttttgaccta taggaagttt gcaaggctgt gtctcaagag
     6001 gaataaaatc cctgtcttcg acagtgattt gtctcctttt tttaacgact aactcttttt
     6061 ccatacgata aagttttttg tcgttggcta cttcattgat accttgatgg tatggactag
     6121 gtgtcatcaa agttggtaat ggtttttgag attgaggacg aatgatatta accttaataa
     6181 gatcaagttg gccatcttga tcggtatcaa gaggtgcttc aacgtaaaca acttctcgaa
     6241 tcagctgtga cgtgtcaaag gtagctaatg atttaccgtt gaagaaatgg taatcattgt
     6301 ccatagtaag cattccttgg ctgaccaaat catcaatcag ggtcatgcca gacttacaac
     6361 gggtcgctaa taagtggtgg atatgcttga taatttctgt ttcgtcaaag tggataggaa
     6421 aggccagttt atcaaggaaa gcatcggcct ctgtaaaatc aactccagga ataaagccaa
     6481 ggacttgcag ggccagtaaa tcaaaaacct ctttggataa ctcttgctct gattgaaaaa
     6541 agctcaatgc gtccatgtca tttttggcga ttagatgact taaaggataa tcactatcag
     6601 ggtagtggaa gaagatttta cgtaaaaagc tttctaggtt agctttggca gttttttgca
     6661 gatccagatc aaagcccaat tcttttaatt cttcagccgc tctttctaga ctagttggaa
     6721 tataggaaaa ttgattatag cgcataggag tttcctttct agtggcttag aaaacgatat
     6781 tatcttttaa tttatcaaga ttggtaatga taatatgttg tttatcaatg cgaatgatgt
     6841 ttttttcttt caactgtttt agaatacggc tgacgctgct agcggtagaa atgccacaga
     6901 atttaccgag ttcctcatta gtaatgacaa aattaattaa aatttgacca tttggtagtt
     6961 cttcgccaaa tagggtcatt aagtcatata tttgagtaga aatggcaccg atacggccgt
     7021 tagttaacat acattgcatt tttttcatgg atttttgaag acgattatgg taaaagtcct
     7081 tcacatatcc ttgtagttca atatcttcgt ttatatcttt taaaaatgct gagcggcgaa
     7141 ccttgtaaaa actagctttc tctgactcga tgcgaacatt ataaggctct cccatatctt
     7201 ttgaataacc agtgttgaga acagaagtaa tttcaagtcc tgtaacatat ctcaaattaa
     7261 attccattcc atattttgaa agaacactct gtttcacaat gccgtctttt aaaatataag
     7321 tgaagtcctc ttcgagactt tcaaaagtca agtacttgtg ataacttttt tcaatgatag
     7381 gaaagttatg attatcaata taccgttgca aaatgtgatg attcacgctt ttctccaaat
     7441 attcttttac caatgtataa tttgtatctt caagagaaat taacatttgc taatgccctt
     7501 tgatagtaac acttatatta taccattgaa atatattata ttctgctttt tttgtaaaat
     7561 gtttatgaaa atagagagga gttcattttc aatggaagaa aaattgttta acaaacattt
     7621 tgtggccatc acagtaataa actttatagt ctatatggtc tactatcttt ttacggttat
     7681 tattgccttt gtggcgacta gagagctagg agctcagact agccaggcag gattggcaac
     7741 agggatttat attctaggga ctttattagc ccgtttgatt tttggaaagc agttggaggt
     7801 gtttggtcgt cgtttggttt taagaggagg tgctattttt tacctcttaa cgaccttagc
     7861 ctacttttat atgccaacga ttagcatgat gtacttggtt cgtttcttga acggatttgg
     7921 ttatggtgta gtatcaactg cgacaaatac cattgtaaca gcctatattc cagctcgtaa
     7981 aagaggagaa ggaattaatt tttatggctt gtcaaccagt ctagcagcag ctattggccc
     8041 ttttgtagga acctttatgt tggataatct tcatattgat tttcgcatgg ttattgtctt
     8101 atgtagcgtc ttgattgggt gtgtggttgt tggtgctttt gcttttccag tcaaaaacat
     8161 gtctttaaat gcggagcaat tggctaaaac caaatcgtgg actgtggata gtttcattga
     8221 gaaaaaagcc ttgtttatca ctgcaatcgc ctttttaatg gggattgcct atgcttcagt
     8281 tttaggattt caaaaattat acacgtctga aattcatttg acaacagtag gcgcttattt
     8341 ctttgtggtc tatgccttaa tcattaccat cactcgtcca gcaatgggac gtttaatgga
     8401 tgctaaaggc gataaatggg tcttgtaccc aagttatctt tttttagcaa tgggactctt
     8461 cttgctaggt agtgtatcgt ctggaggaag ctatttgctt tcaggagcct tgattggctt
     8521 tggttatggc acttttatgt cttgtggtca agccgcctcc atacaaggag tggatgagca
     8581 ccgctttaat acggctatgt ctacttatat gattggtctt gatttaggtt taggtgctgg
     8641 gccttacctc ttgggcttga ttaaagattt ggcacttggt agtggtgtgg cttcctttag
     8701 gcacttattc tggttagctg ctgtgattcc actgatttgt accttgcttt acttattaaa
     8761 aacaaagaca agacaggttt tgtcttaagg acaagaaaag gagtttagtc ttatacttaa
     8821 atacaaacgt atcttaacag ctgtagatag ttcctatgag ccgggattag ctgtgggtaa
     8881 agccattact atagcgcttt gaaataattc tagtttgcta ttcgtacatg tcaataaaaa
     8941 tgactcgcaa aagccattcg tgaaagagaa aatgtggcct tatttttagt aggtacaatt
     9001 ggattaagca cgtttagata catgacaatt ggattaacat cagaatatag gctttgtcat
     9061 gttaagattg atctgttgat cgtgagagct aataaaaaat gttataatac tatgaaataa
     9121 agtgataaag ataaggcctt ttatggtcct tttctttacc gcttttatta ttgtgtgaaa
     9181 ataacactta atgatcattt tatttattat tttattacct ttacattcat caataagcct
     9241 gttaaaatgg tatttgttat attttattat ttggaggaaa ccagaaaaca tggaaatgac
     9301 atggactgtg aaatacatca cagaatttat cgcaacggct tttttgatta ttttggggaa
     9361 tggagctgtt gctaacgtcg acttaaaagg aacaaaagga cataactcag gttggttagt
     9421 gattgctttt ggctatggct taggagttat gatgccagct ttgatgtttg gtaatgtatc
     9481 tggaaaccat attaatcctg ctttcacagt tggactagca gtatcaggct tgttcccatg
     9541 ggctcacgtc ttacaatacg ttgtggcaca attacttgga gctatctttg gtcagttggt
     9601 agtggtaatg gtttataaac cttactttat gaaaactgaa aatccaaatc atgttttagg
     9661 ctcattctca acgatttcat ctcttgataa tggtcaaaaa gacagccata aagtctctta
     9721 tatcaatggt ttcttaaacg aatttgtagg ttcatttgtt cttttctttg gtgctttggc
     9781 tttgactaaa aactactttg gggtagagtt agttggtaaa ttgattgaag caggttacga
     9841 ccaaacaact gcagcaacac aaatttcacc atacgtgaca ggctcattgg cggttgctca
     9901 cattggtatt ggtttcttag ttatggtctt ggttacttca ttaggtggac caactgggcc
     9961 agcactaaac ccagcgcgtg attttgggcc tcgtttgtta caccacttct taccaaaatc
    10021 agttcttggt caagccaaag gtgattcaaa atggtggtat gcatgggttc ctgtagttgc
    10081 acctatctta gcggcgatag tagccgtagc agcgtttaaa tacctttata tcagataaaa
    10141 aaagaactaa agcttctttt agactcaaga caagctcgct taaggaactt gtctttttta
    10201 tatgagttaa gcgaaatcta atgcaaactt gactgtcttg tatagcgtat tttgtaaatt
    10261 ttactagact tcttgtaaaa aagcctctcc tcagataaga tgactaatgc ctctaaacgg
    10321 tgttacaaat aaacgaggca tccctaactt cctttatctg gtgttgaaac tattttgcac
    10381 ggactaaatc actgatagag taaaaaacga tttttcattt ttgttgtaaa atattaaaaa
    10441 cgtctctggt tagtgattcc cagaaacgtt atcttatcta tttgtttgtt gcttatgaga
    10501 ggtaaaggag gaacgaatca gcattccacc agtgaagaaa acgatagcga tgaccagaag
    10561 tttcaagcta ttaatatcta aatttgttaa gaaaaacaca gctagtaaaa cgccaataat
    10621 accaccagaa acaattccag caaaaccatc ccaactgaca cggtttgctt tgataaactg
    10681 gctactagaa gctgtcataa tcatagctgc atctagcatc ataacaggca tagcaactgt
    10741 tgggctaaga cccattaatg aaaagaaaat caattctgga gcataattgc ctaaccccat
    10801 agtcatcaag actccaacga taaaattaaa accaattcca acaaaaagcc agatgccatg
    10861 gaggccatga atggtgtctg aaaggtgttc tcctggatgg tagattattc taatgatcat
    10921 gattatagct gctatgatta aaagactacc aaggattcgt tggacagttg gagcatgcca
    10981 gttttttgta atatgagtcc ccaaataagc tccaataaag gctgctgcag ccatagcaag
    11041 taaagttaga acctcaactt ttacgacaaa aataaagcaa agagattgaa tgagcaccgg
    11101 aagcacatga gctacggtca tagtgccagg caattgtcta tcgtcagtga ctaatttagt
    11161 taacttaaag cacgttgtgg tagtagcaaa agaccctatc ccaagggtgt caagggcatc
    11221 tgttagcagg ccaatccaaa accccgtcca aaagcgttta aaaggattga tcttattttt
    11281 tttaagatgt tttactaaca taaagaaaat aaaaagcatt gccgaaacta gcaaagcttg
    11341 tatcagtcta aggataagca tttcgttcat aaagactagt ttacaaaaaa aaaccttagc
    11401 cgtcaacgtg atttacttca ttaaaacatg ccactatgct tagaagagct ttggttcgat
    11461 gtattttgtt aaaattaagc taaaatatta atattgcatg aaaggggacg ccttcctttt
    11521 tcccttactg agcttagctg ttattgtagt gaatgtttaa aaggctagtt tgtcgctctt
    11581 tatcaaaaat ctagtaggat gaatacgtat aatttcttgt caaatagagg aattttgtct
    11641 ataatagcag aggattagga ggaaattatg actttaatgg aaaatattac ctttaaaact
    11701 ccagtattac gcgttaatga tcgcgacctt aatattgcat tttatcaaaa taatttaggg
    11761 cttcgtttag tatctgaaga aaatgcgatt gctatctttt catcttgggg agaagggcag
    11821 gaatgttttg taattgaaga gtcaccttca gttagaacgc gtgcggtaga aggccctaaa
    11881 aaggttaata cgattgtcat caaaacaaat caacctaaag aaatcgaaca attattagct
    11941 catggagcac attatgacgc tctttttaaa ggtcaaaatg gctatgcctt tgagactatt
    12001 tccccagaag gagaccgatt cttgttgcat gctgaacaag atattaaaca tctccaaggt
    12061 actgatttac catcgcttga aaaagatgca acttttaaag gtttaactca atttaagttt
    12121 gatattattg ttttaaatgt tattagtgaa gaacgctcaa aagcttttta tcgggatctg
    12181 tttagtgatc agttacctat tactatggat tttattcaag aagaaggtcc cgatttggca
    12241 attgatcccc atattgcttg ggatttagaa attttagaat ttcaagtctc aaaggactat
    12301 gatatgaagg tcctaaaagc aactttagaa gaagacgggc acaaggtcta cattgataaa
    12361 aaacacaaag tattggtcct ttctgatcca agtcagatcg aagtatggtt tacgaaatga
    12421 ctttggcagt cattaaatgt attgaaaacc atcttcacaa aaaagtatac aaaggggcca
    12481 gtctggctct ctttcaatct ggtcgatggc aagaatacca tatcggaaca attgatggaa
    12541 gacgaccagt tgatgctaac ttagtttatg acttggctag tgtttcaaaa gttgttggtg
    12601 tggccacaat atgcaatatt ttattgaata atggtacgtt agcattagat gatcccttaa
    12661 aagcatatta tcctagtatt gttgatgcga ctgtcactat tcgacagtta ttgactcata
    12721 ccagtggtct tgatccttat attcctaaca gagatgtttt aaatgcacaa caattaagaa
    12781 aagcacttaa tcatcttact caaaaaggaa ataaaaactt ttattacaca gatgtcaatt
    12841 tcctcttgtt aggctttatg ttagaagagc tctttagtga atctttagac cagatatttg
    12901 acaagactat ctttactccc tttgggatgt atcatacgtc ttttggccct cgtccagaag
    12961 ctgtaccaac attaaagggt gtgagtgatg gggaagttca tgatcccaaa gcaaaaatct
    13021 taaaaaaaca ttctggatct gcggggctat tttcaactct tgcagattta gaaagtttta
    13081 gcaatcatta tcttaatgat ccattttcag attgtctgtg gcgcaactac agccaacaaa
    13141 caattgagcg gtcactaggg tggaatttag atggcgattg gattagtcat acaggttaca
    13201 cagggccgtt tctaatgctt aataaaaaag agcaaacggc agctattttt ttgaccaacc
    13261 gtacttacga cgaggatgat aaaagtaaat ggttaaaaga acgtcagttg ctttacaatg
    13321 cactaaagca tgatctgaca acacccgtat cgtaacgtct aaaggggcag ttttagcgat
    13381 cttattactg tttttaaagt ttcttctaaa aaatagccat ttcatttgtc tgatgagatt
    13441 agagtgatta taagtgaaaa aagaacagcc atctcatttt gaatcaaatg agatggctgt
    13501 tcttttttat aagttattga tcatttagat actactattt acttgaaata gcttcagcgg
    13561 catcatccat tgaaagcact tcgtggaaga cacgttgagt caattcagtt ttttgttctg
    13621 gtgtaaggta tttggtgttg acacagtaac cagagatacg cacgataacg tcttcgccgt
    13681 tcatgatttt gtcataaaca tcgttaaggt ccataacgtt caagttaacg tgctgaccac
    13741 cattttcaaa gtaaccgtca agcactgtta ccaagttgtc aacttgttcg tcaaatgttt
    13801 taccaagagc acgaggtgat acttgagtag tcaatgagat accatcgtta gcatgtgaga
    13861 attccagttt agcaagtgag ttaaggtttt gtaaccagcc accttttgct ttgttagatg
    13921 ggttagcacc tggtgagaag aattccactt ggctagtgtt aactgtacca tcttcgttta
    13981 agaagacgcc gcggtgaact ggagagttac cagtttgttt agagtaagca acgtttgaag
    14041 tgatggtaag aagtgatact gaagcctcag cattcttgta aagtttgtgg cttgcaagac
    14101 gtgtgtggta agcttccatc aaccatttcg cgatgtcatc aacacggtcg tcatcttcac
    14161 cgtaacgagg gaagtcacct gttacttcgt agtcatagat gtagccattt tcatcgcgga
    14221 tagttttaac agttgcgtat ttaatagctg acaaggtatc aactgtgtta gcgaaaccac
    14281 agataccgaa tcccatgtta gcacgttggt gtgttggcaa gaaggccatt tgaactgctt
    14341 cgtagttgta tttatcagtc atgtagtgga tgatgttaag cgcatctaca taagtatctg
    14401 tcaaccagtc aagagatttc tcaaagttag ccataacttc gtcgtactca aggacttctg
    14461 atgtgattgg ttcaacaacg ttaaacactt tgtagtctct gtgaacatca tcgtaaccac
    14521 cgttaagacc agtaagaagg gctttgagaa cgtttacacg agcaccgaag tattggatgt
    14581 tatgacgttg ttcttcattt tctgggtcaa gtggtgatac acaacatgaa atacatgaca
    14641 tttcaccata accttcttta gccattgttg tcacaccttc atattggata gaagagtgtt
    14701 tgtggctcat tttcatacag tagcgacgga atgtttctgg cagttggtca gtccaaagaa
    14761 ctgttaagtt tggttctgga gagttaccga tattatcaag agtgtttaag aaacggtagt
    14821 ccatcttagt cacacggtga cgaccgtcgt tacccatacc agccatagaa gttgtgatga
    14881 atgttgggtc acctgagtaa agggcatcgt aagctttagt acgaccaaat tttacggtac
    14941 ggagtttaag gacaaaatca tccacaaatt cttggatttc tgattctgta aaggtaccgc
    15001 gagcaaggtc acgttctgca aagatatcaa gaacgattgg cacacgtcca agagaagtag
    15061 cagcaccgtt gatcacacga catacagcca tgaaagcgat gtttacccat tggatagctt
    15121 ctttaacgtt ttgtgctggg cgacgaacat caactccgta aaggtcacca agtttaacca
    15181 cttcaccaag agcttggtat tgaaggttaa cttcttcgcg aagacgaatg gattcttcat
    15241 cgatttcagt gatagcattc cagtcattga ctttttcttg catcaagtag tcagcaccgt
    15301 aaagagcaag acgagcgtaa acaccgatga tacgtccacg tgagtaagca tctggaagac
    15361 cagttacagt gtgggcatga cgagcacgac gaatatttga agtataagcg cggaagatac
    15421 cgtcgttaac agttgtcacg tacttagtaa agatttcgtg tactgctggg tctggttcat
    15481 agccgttttc tttaagggtc gtttccgcca tacggatacc accttttggc atgaagttca
    15541 atttgaacaa ttcatcgttt tgaataccat aaattaattc attttcttta tcgatgaaac
    15601 cagcaggaat gtcagcaatt gaagttggac gagtatcgta tgggaaacgt gtagcttcat
    15661 agtgtgcttt tgtttcttcg ataacttttt tgatgtgaag tgaacgttca gtagcaccag
    15721 ccaagaaaga ttcatcgcca tcgtatgggg tgtagttagc ttgtacaaaa cgtgagacac
    15781 ttgctttttc tttccagtca gttcctttaa agccttccca agctttttcg aaaacatctg
    15841 tattggtttt aacagttgcc ataaatttct ccttaaattc tagtaacaaa gcgatctgtt
    15901 acatttacaa ttctaagttt aacatatagg aaacgttttc ccaagcaaaa ttcttgacaa
    15961 aattaagatt cttttataat acagattgtt gaaacagtca caaactgtat tagaatgtat
    16021 ttttttggct ttagatagcc taaaaggtat aatagagaaa ggagggactt atgcttattt
    16081 ttccactgat taatgacacg tcacgaaaaa tcatccatat tgacatggat gccttttttg
    16141 ctgcagttga ggaaagggat aaccctgctt taaaaggaaa gcctgttgtg attgggaaag
    16201 atccaagaga aacaggtggt cgcggagttg tttccacttg taattacgaa gcgagaaaat
    16261 atggcattca ttcggccatg agctctaagg aagcttatga gcgttgtccc aaagccattt
    16321 ttatttcagg aaattatgaa aagtatcgaa cagttgggga ccagatccgc cgtattttta
    16381 agcgttatac tgatgtggta gagcccatgt ccattgatga ggcctatcta gatgtcacca
    16441 acaataagtt gggtataaaa tcagccgtca aaatagccaa gctgattcag catgatatct
    16501 ggaaagaagt aggattgacc tgttcagcag gtgtgtctta taacaaattt ttggctaaat
    16561 tggctagtga ttttgaaaaa ccccatggcc tcaccctagt cttgaaagaa gatgccctgt
    16621 gctttttagc caaactcccc attgaaaagt ttcatggtgt tggtaaaaaa tcagttgaaa
    16681 aactgcatga catggggatt tatacaggac aggatttgtt ggcagttcct gaaatgacct
    16741 tgattgatca ttttggtcgg tttggttttg acctttaccg taaagcgaga ggcatcagca
    16801 attctcctgt caagtctgat cggatacgca agtcaattgg cagtgagaga acctacgcta
    16861 aactgcttta tcaagaaaca gacatcaagg cagagatcag taaaaatgct aagcgcgtgg
    16921 ccgctctctt acaagaccat aaaaagttag gcaagactat tgtactcaaa gtgcgttatg
    16981 ctgattttac caccttgaca aaacgtgtca ccttgccaga attaaccaga aatgccgcac
    17041 aaattgagca agtagctggg gatatttttg acagcttaag cgaaaatcct gctggtatcc
    17101 gcctgttagg gataaccatg accaatttgg aggataaggt agctgatatt tccttggacc
    17161 tataggagaa agagtgctct atccgtccgc ctcattttgg agaaaaaaga agctgattag
    17221 ggatttccct aatcagcttc tttggtccat tattttatta cagcatagct tggtctgtat
    17281 catcaccttt ttcgttaagg gttttagcct tgtaaaaagc aaagccataa agagcagctt
    17341 gtataaggat aaggatagtt ccaaccattg acatggttgt tgtgaactgg ttaaccaagc
    17401 cgataacaga aaggataagg cctaagatat aaggaatata actaaccgtt agaccttgtt
    17461 ttaatttaga taagttttga gcacaaaaga caatgatagc aataatagca aggacattta
    17521 agatagataa gaaaatcata aatggtgtca tgctgctttg tagctgtgcc aattgctcag
    17581 ttgttaattg ggcccgaaat gcttcattct tgagttggct ttgtaagtag aaaatccctg
    17641 caaatcccat taatgacagt acaactccaa gcccgtttag aataataatg gcaatagtag
    17701 acgttttgag tgcctgacgc actttttcat aagaaatcat ataatcctcc tagagttctt
    17761 aatccgaaat tgtacaatgt gtttaataca aaaatattat accatagatt aatcaaaaat
    17821 gtccgttttt ctaggatttt ttctggaaaa agagggcaat atctgactct gtaagcccta
    17881 tcatcgaatc gatagtcgaa taattctcag gtgttaacct aaaatcatca ttatctgact
    17941 gagagttctc agtggggtta gagtttgttg attgagagga ggctaagtcg gattgttctt
    18001 ggaagcgttc gatcaagtag gtttggcgtt tatcgccttc gtgtttaata gcatactcaa
    18061 aggcagtata ttctcccaac agaattaact tacttttaga ccgagtaatg gccgtgtaaa
    18121 tcacatttcg ttgcaagagt cggccgcttt ggcgtgtgat aggtaaaatc accacttgaa
    18181 actcactccc ttgcgattta tgaatgctca tggcataggc cagggttaat tttagccatt
    18241 catttctagg gtacgtgact tcgctaccgt caaaatctaa gattaattcg tcttgcttag
    18301 attcggtgta tttagcagga atcaaatctg taatataacc aatatctccg ttaaagacat
    18361 tcaactgagc atcgttgact aagtgcagga ctttatcacc tttacgaaaa tgcgtatcat
    18421 tgaacagaaa ttctgtttgt ccttgcaagg gattgagaag ttcttgcatg agttgattaa
    18481 gatgattgat gccagcttgt cctttgtaca taggcgctaa aatttgaatt tcctcagcag
    18541 ggatgccact tttaatagca gataggacaa ttttttgaat catgtcaggg ataaaggctg
    18601 cttgggcttc aaaataagaa cggtcagctt ttttatcacg aaagtcagca gctaagattc
    18661 ctcgacgcat ctggtctgcc aaattgacaa tagtagattc ttgggattga cgaaatatct
    18721 tttgcaaggc gatttggggc aggctattga cttttaaaag atctgacagg acttgaccgg
    18781 gtccaaccga aggaagctgg tcactatctc cgacaataat cacttgagtt gttgaattaa
    18841 tagctcctaa caactggttg gcaagccagg tatccaccat tgaaaattca tcgacaatca
    18901 gcaggtcgca gtctagataa tcctccatgg cttggtaatc gttgtcgcca ttgaggccta
    18961 agtgcctgtg gatggttgcg ctaggaagtc cagtcaactc attcatgcga cgtgcagccc
    19021 gacctgttgg agctgctaac aagataggaa ggtctttttt atccaaatca atctgatgca
    19081 ggttcgcgta agcctgcaaa atgcctcgaa taacagttgt ttttcctgtt ccgggaccac
    19141 ctgttaggag aaagaccttg ctggttaagg ccttagtaat ggcttcttgc tgcacttggt
    19201 cataggtgat ggcaaagtct ttttgaacgg cttggatggt tgtttgaatg gtatcatgac
    19261 taaatgactg acttaaaggg gtatctaaga gacgagagat gttgttagca atcccttctt
    19321 cagcaaaata aaggctagca tcaaacagtt tggtgtcact gtttttgatt ttgccctcta
    19381 tgatcaactc gcttagttgt tcagccacag cagcaggatc acattctact tgacgtgcat
    19441 cttcaaggag tgtaatggca aagtctaata attgtcgtgc ttgaacataa gtatctcctc
    19501 gattgatgga ttcttccaag agacagtgaa ggagggctgc acgaaaacgt ttcggagagc
    19561 cgctttcaat ccctaagttt tcagcgagag catctgccat tttaaaccca aaaccttgta
    19621 gatcttcgac taactgatag ggattttctt taacaaggtc aagagcctct tctttatact
    19681 tttcaaacgc ttgaaaggca aaacggttgc taaggccaag ttcgacgaga ccagcaatca
    19741 gttgctctgt gccgtaattc aattttagtt tagcgacaaa ggcttgacga ttagccttag
    19801 ataggccaga gatagtttct aatttgcttg ggtcttctaa aatatggtca atggtattat
    19861 gaccatatag tgcaatgatt ttctccgctg tttttttacc aatcccctta aaatggtcgc
    19921 tagagaaata attaaccaaa ccagatgaac taggtttgat tttttggtaa cggcttagtt
    19981 tgagttgctg tccatattta gggtgctggg tcaattctcc ccaaaaggtg tagtcatctc
    20041 cttcaataat gtcagccatc gttcctgtga taatgatttc aaagtcgtca atgtcactgt
    20101 ctgtatcctc aatggcaagg aggagaatct taaagaaatt agcctggttt tcaaaaatga
    20161 tacgatcaac agtacctgta aaaacgtatt ccatagtaac tctttctatt tatacaagat
    20221 aactgaaaac ctgcccaaag gcaggttttt tctaattaaa gacggtcatt ttattgagtg
    20281 gccaaaaacg agctttcact tcaccgataa ggttttcttt tttgaaacta ccaacttcgc
    20341 gactatccct ggaaacaata cgatcatcac caagaagaag gtattctcct tttggaacac
    20401 tcatctcaaa gcgtgtttgt ccctcagaat tagttgtaaa agcatcagat gtttctgcta
    20461 actgttggaa tagggtatta taggcgtaag ttttttggag tttatcgttt ttaaattgtt
    20521 ttagatactc agccaaatac ggctcaactg tttttttacc attaatataa agtgtgtcat
    20581 cattataaga aatggtatcg cctggcaatc caataactct ttttacgatt tctttctttt
    20641 gtccattttc ttcttcctga gcaactacaa tatcaaagcg atcaatccta gcttgattaa
    20701 aaacgataag gcgttcgcca tgagctagag ttgggtccat agaatggccg tctactttaa
    20761 cagcctgcca caaaaaaaga cgagatagac caaagagaat taaaaagaga gtgaatgggc
    20821 cccattcttt aataaactgt ttcatatgat tccttttatt tagtgagaag cgcttgcgct
    20881 ttgtgagtgt tggcgaagtg aagtttagca ctatattcta agctagacat accataagcg
    20941 gctaagagtt ggctagcaac tttatctgaa gctgaaccag caccgcttgg gagttggtaa
    21001 cctaaatctt gacctaattg atcgagattg tctaaaaata gatttctagc aatgatagag
    21061 ctaacagcca cagccaaata gtgactttct gccttttctt gaaaggttag aggatttggg
    21121 aaatggtttt tttccttttt tagatgcttt tcataatttg attggctagt gaaagcatca
    21181 ataacaattt gtttaggttg aatgcctttt tgtaacaaca aaaaaatagc ttggttatga
    21241 agagcaactt tgatagaaat ggtattatat ggtttacttt taccaaccaa ttcgttatat
    21301 tttttaggtg ataggagaag tgattgatga ggtatttgtt tctctaacag aggcgcaatc
    21361 tgttgaatgg ttttatccga taatttttta gaatcatcaa ccccaagctt tttcaggaag
    21421 ggatgatctt taggatcgac aaaacttgcc acaacagcaa tcccaccaaa ataagagcca
    21481 ttacccacct catcactgcc gattataggt atatcttggc tattgttgct tgcttcaaaa
    21541 actgtcttag ctactggaag attaagctct tgagctaaag catttgcacc gtttccctgg
    21601 agaactaatt ttccggattt gtacagcaaa acagtaaccc catttttttt agcagcaaag
    21661 gctacataga tattttggct actgatggtg tagggggcta gctgtttttt taaatgttta
    21721 ctcagtatag catctatttt caaaactaag gtattcataa taattatttt aacataaaat
    21781 agttgactat ggttaattca taagtttatt taacaaagaa aacagagact cgaatgttta
    21841 gacgggttag ggcaatggtg ttgactattt tacgaaagtt tcattttacg gtataatgct
    21901 aatgaggtga ccctatgaaa agtattaatc gctataaatt cacctttggt gaaaaaacat
    21961 taacattgac aactgacaaa gacaatctct ttatggaaga agttgagcgg gtagctaaag
    22021 aaaagtatca ggctcttaaa aatcatttac cagaagctga tgacgagaca atcgctattt
    22081 taatggctat caataccttg tcaacacaat taagcagaga gattgccatt gaaaaaatgg
    22141 aagctgaaat tttagattta cgtcaaaaaa ccttggttgg tcttcaagaa aaagctaatc
    22201 aaatagatgt gaatgaggtt taatcatgtt atctttattg atagtgcttg ttttaacttg
    22261 gaatttttat attggttata gtagaggtat tattcttcaa tcgttttatg tcttaggagc
    22321 cctactttct cttttggtag ctaatcgatt ttatattgga ttagcccata agctaacgct
    22381 ttggattcct tattctaacc cggtagaagg aacatcagtc tttttcttta aatcagttga
    22441 tatctttgtt ttagataagg tttattatgc aggccttgcc ttttttatca ttttcttatt
    22501 ggggtatgca ctgagtcgct ttcttgggat ttttgttcat ttccttttgc tagattattt
    22561 tgataaccag tggacaaaat gtttgagtgg cggactagcg cttctagtat cgcttctttt
    22621 tttgaacatg ttactgtcta tttttgcaac ggtccctatg ccttttttac agcactattt
    22681 gcactctagt tttcttgctc gtttggtgat tgaacacctt ccaccattga caataattat
    22741 tcaaaaattg tggattcaag ccattatttg atatcgtttt gtgactaata taagaacctg
    22801 gagtgagttt cacctttagg ttctttattt acgagaaact gtttagaaat aaagagaacg
    22861 catgggaact tagtgacttt cttgccacat tttccatcat tttaggtata atgaagagta
    22921 tgaataacaa gattttagag cagttagaat ttaacaaagt taaggaattg ctattacctt
    22981 atctcaagac agaacaatca caagaagaat tattagagct ggagccgatg acggaggctc
    23041 ctaaaataga aaaaagtttt aatgaaattt ctgacatgga acagattttt gttgaacatc
    23101 actcatttgg catagtcagc ctaagttcaa tctctgagag tttaaaacgc ttagagcttt
    23161 cagctgatct taatattcaa gaacttttgg ctatcaaaaa agttttacag agttcttcgg
    23221 atatgattca cttttattct gatttggata atgtttcttt ccaatctttg gatcgtttgt
    23281 ttgaaaattt ggaacaattc cctaatctgc aagggtcttt tcaagctatc aatgatggtg
    23341 gttttttaga acattttgcg agtccagaat tagagcgtat ccgtcgtcaa ttaacaaata
    23401 gtgaacgacg ggttcgtcag attttacagg atatgcttaa ggaaaaagca gagcttttat
    23461 cagagaatct aatcgctagt cgtagtggac gaagtgtcct accagtaaaa aatacttatc
    23521 ggaatcgtat ttctggtgtg gttcatgaca tctcttcttc aggaagtact gtttatattg
    23581 agcctcgtgc tgtagttaca ctaaacgaag agataactca gcttagagct gacgaacgcc
    23641 acgaagaagg tcgtatttta cacgcatttt cagacttgtt aagacctcat gtcgccacta
    23701 ttagaaataa tgcatggatt cttgggcatc ttgattttgt aagggctaaa tatcttttta
    23761 tgtctgataa taaggcgacg atacctaaga tctctgatga cagcacgtta gcattaatca
    23821 atgttcgtca tcctctttta agtaatcctg tggctaatga cttacatttt gatcacgatt
    23881 taactgcaat tgtcatcact ggtcccaata ctggtggtaa gacgattatg ctaaaaacac
    23941 tcggtttagc acaattaatg ggacagtctg gtttgccagt attagcggat aaaggtagta
    24001 aaattgcagt atttaacaat atctttgcag atattggcga tgagcaatct attgaacaaa
    24061 gtctatcaac tttttctagt catatgacgc acatagtcag tattttaaac gaggctgacc
    24121 gcaatagttt agttctcttt gatgaactag gagcaggaac ggatcctcaa gaaggtgcta
    24181 gtttggctat ggctatttta gaacacctta ggttaagtca tatcaaaacg atggcgacca
    24241 cgcactatcc agaattaaaa gcttatggga ttgagacaaa ttttgtagag aatgcgagca
    24301 tggaatttga tgccgaaacg cttagcccca cgtatcgctt tatgcaagga gttcctggac
    24361 gatcaaatgc atttgaaatt gcttctcgcc ttggtttagc tccatttatt gttaaacaag
    24421 ctaagcagat gacagattct gactcagatg ttaaccgtat tattgaacag ttagaggcac
    24481 agacacttga gacacgtaga agactggatc atattaaaga agttgaacaa gaaaatctca
    24541 aattcaatcg tgcggttaag aaactctata atgaattttc acatgaacgc gataaagagt
    24601 tagaaaaaat ctatcaagaa gctcaagaaa ttgtagatat ggctttgaat gagagtgata
    24661 ctatcttaaa aaaactcaat gataagagcc aattaaaacc tcacgaaatt atagatgcta
    24721 aggcacaaat aaaaaaatta gcacctcaag ttgatttatc aaaaaataaa gtcttaaata
    24781 aggctaaaaa aatcaaagca gctcgtgccc ctagaattgg tgatgatatt atagtgacta
    24841 gttatggaca gcgaggtacc ttaactagtc aattaaaaga tggccgttgg gaagcacaag
    24901 tgggaattat caaaatgaca ttaacacaag atgaatttag cctcgttaga gtccaagaag
    24961 aacagaaagt caaaaataaa cagattaatg tggttaaaaa ggctgatagt tctggaccaa
    25021 gagctcgact tgatcttaga ggtaaaagat acgaagaagc aatgcaagag ttagatcatt
    25081 ttattgatca agcattgctt aacaatatgg gacaagttga tatcattcat ggtattggta
    25141 caggcgttat tcgtgaggga gtgacaaaat atcttcgtcg tcataagcat gttaagcatt
    25201 ttgcttatgc ctcacaaaat gcagggggat ctggcgccac aattgtaacg ttagggtaaa
    25261 tgatggaatc atatgaacac ttttatgcca aactatctca accttttaga aaaaaaccac
    25321 agttaataat tcttttaaat gttttactaa aaataatcac aggagtgatg tacatacttt
    25381 atccatcttt tctgatattt acgctttggc aagggatgac atttcaatta tggttaaggt
    25441 tattaattat tcctgctgtt ggctttatag ctttgtctta cattaggaaa cgctttgatt
    25501 ttccacgacc gtatgaaaaa tggaatatta aacctttaat tgataaggat acaaagggaa
    25561 ggtcaatgcc tagcagacat gttttttcag ctacgatgat tagtatgtgt ttgttacgat
    25621 attatgttta ctttggaata gtttgtttaa tattatcggt tttattggca atttgccgtg
    25681 tgatagcagg tattcactat cctaaggatg ttattgtagg ttaccttatt ggcttgatgt
    25741 tagggctttg tttattgatt tagcgagttc gtagcaaata gtttcaaaaa caactaaatt
    25801 cttgtacaat aggcttatca ttaagatgaa atggagaaaa aagatggcat tagaagtaac
    25861 agatgctaca tttgtagaag aaactaaaga aggtttagta ctcattgatt tttgggcaac
    25921 ttggtgtggt ccatgtcgta tgcaagcacc aatcttagaa cagttatctc aagaaatcga
    25981 tgaagatgaa ctcaaaattt tgaaaatgga tgttgatgaa aatccggaaa ctgctcgtca
    26041 gtttggtatt atgtcaattc caacattgat gtttaaaaaa gacggagaag tggttaaaca
    26101 agttgcaggt gttcatacta aagaccaact gaaagctatt attgctgaat tgagttaact
    26161 aaaataaagc ccagcgtcac gctgagcttt attttatgac ctactaactt caatggaata
    26221 accatgtaaa cataaaacga cttgcaaaag caaaagcaac agtaatgagg aaaaatacag
    26281 tgacaataag gggtttctga taaggaagtt ttgctctttc ttctcgtttt tcgatttcgt
    26341 ctaataattt tccatttgaa atagctagga tttccttata aggttgcaca tcatattttt
    26401 tcttaaaacg ttccacacga ttagcataag tcatagtaat tatcgataat aaaacaaaaa
    26461 tgccaagtcc tagccactct aagaaataga cttaatggat aacttgaaat cattaatagt
    26521 atcatcaatg ttaccattac tttggtatct cgtttaaaat tctttttatc aacctgagtg
    26581 atggtatatt tcattttctc aatgtctcct ttaatgagtt ggtctaagct cacttgaaaa
    26641 atttgactta ggagtaagag gctatggatg tctgggtaag ttttgtcatt ttcccagtta
    26701 gatatgctct gacggcttac atagactttc tcggctaatt tttcttgcga gagcgcttgt
    26761 tgtttacggt agcgaataat ttgctgacca atttccataa atatcaccga aaaactttac
    26821 agcggcgtaa aggaattgta aacaaaaagt gaaccgttat agttcacttg cgattaagca
    26881 tttttctgct ttaagtaggt ctctagcatt ttcttttgcg gtgttgcaaa gggatacaga
    26941 gcaaagtcct caattgctac ccactttagg tgaggagcat taggtaaatc tgttgctttg
    27001 accaccccct ctatgagttc tattgtccac ttctggtgac taaaagtgtg cttaatattt
    27061 ggaaagtgat tatcagtcca ttggggcttt aattgatatt ctctttcgaa agtttcattt
    27121 tgggtttgcc agattatggg gttggattga ttgtcatcaa ataaatctaa ttgttgagaa
    27181 agaggtgacg tttcgataat aggaaatgac caaaaacctc ctagaagtct ccctttagta
    27241 tttttctcca gtagatattg gccattttgg ttacgaatga caaaagcctg aattcgcatt
    27301 ggtttaggct ttttctttgg attttttatg gggtatttac cataagtacc attcaaataa
    27361 gcagcgttaa agaaacgaat agggctttca tcaggcctag gtgtcttagc ggattcgatg
    27421 tctgtaccta gatccataag tgcctggtta aaatcaccgg gtctgtcagg gtcaattagt
    27481 atttccataa tagcttgaaa aattttacga tttttagggt caccgatatc ataattaact
    27541 tcaaacaaac gagccatgac acgcatcaca ttgccatcaa cagcaggctc tggtaagttg
    27601 aatgagatgc tggcgatagc acctgcagta taaggaccaa tcccttttaa tgaagcaata
    27661 tcatcataag tgtgaggaaa tatgccacca aaatcaacca tcacttgctg tgcagccttt
    27721 tgcatattac gcacccgaga ataatagcct agtccctccc atgcctttag taattgttct
    27781 tcaggagcgt ctgccaaatc ttttatttga ggaaaccact ctagaaaacg tttataataa
    27841 gggattaccg tattaacttg tgtttgttgc aacatgattt cagaaaccca aatataatat
    27901 ggattagtag ttcttcgcca agggagatca cgtttttctt ggtcatacca ccctaaaagt
    27961 gtgcgacgaa aagaagcgat tgtctcatta tcccacatat taataccata atcttttaat
    28021 tcaatcatgt tgttattgta acacaggagc cttaaaaaga gtaaaaaaag attttaggta
    28081 tttgcgaaaa agaaaaggaa atattataat aatattaggt ttacctcatc taaatgttga
    28141 tttagaataa ctgaaattaa cgatagaagc agacaggttt taatttggag gggacatgtt
    28201 ccagaaatta aagaatctta ttgatcggta ttcgtggttc gtttttataa tgatgattct
    28261 tgtgttattt cttaactata tttatccatt tgatccttct ttgagcggac tacagttgga
    28321 atggctgatc atgacgcaag tttttcctgg attaggtatc gtatgtgcca tggttgctat
    28381 tatttttaaa aagtttaacc tttttgtttt ggccctaatt ttatattttg cactttggtt
    28441 aaaacttttc cttgcttttt cttttttcac ctatttttta ggagtctgac taagtgccaa
    28501 tttttaaaac tcgtgaaagg aaatgtttga caaaacttag tcaagtagaa aaagttcctt
    28561 gcttttaatt gactattagt gtacaataag attctgtgag tacacctcac ttactcactc
    28621 caaaagtagt gagtcattag accaaaagga ggaaaatatc aatggctaaa tacgaaattc
    28681 tttatatcat tcgtccaaac attgaagaag aagctaaaaa cgctttggta gcacgctttg
    28741 actctatctt gactgacaac ggtgcaactg ttgttgaatc aaaagattgg gaaaaacgtc
    28801 gtcttgcata cgaaatcaac gatttccgtg aaggacttta ccacatcgtt aaccttgaag
    28861 cgactgacgc agcagctctt aacgagtttg accgtctttc aaaaatcaat ggtgacattc
    28921 ttcgtcacat gatcgttaaa cttgacgctt aattccaact aagagggtag tttatgatta
    28981 ataatgtagt actagttggt cgtatgacca aggatgcaga acttcgttac acaccaagtc
    29041 aagtagctgt ggctaccttc acacttgctg ttaaccgtac ctttaaaagc caaaatggtg
    29101 aacgcgaggc agatttcatt aactgtgtga tctggcgtca accggctgaa aatttagcga
    29161 actgggctaa aaaaggtgcc ttgatcggag ttacgggtcg tattcagaca cgtaactacg
    29221 aaaaccaaca aggacaacgt gtctatgtaa cagaagttgt tgcagataat ttccaaatgt
    29281 tggaaagtcg tgctacacgt gaaggtggct caactggctc atttaatggt ggttttaaca
    29341 ataacacttc atcatcaaac agttactcag cgcctgcaca acaaacgcct aactttggaa
    29401 gagatgatag cccatttggg aactcaaacc cgatggatat ctcagatgac gatcttccat
    29461 tctagtcaga atggattgaa cgaatacgaa attatttgag ttcgggctaa aaatttagaa
    29521 cttagaattt agatcaactc cctaggaaat caagatttct tgccagtttt ctaaaattca
    29581 gtcgtttttt aagagccctt aacatcataa tttataaagg agaaaataca tggctcaaca
    29641 acgtcgtggc ggattcaaac gccgtaaaaa agttgacttc atcgcagcta acaaaattga
    29701 atatgttgat tacaaagata ctgagcttct tagccgtttc gtttcagaac gtggaaaaat
    29761 tcttcctcgt cgtgtaacag gaacttcagc taaaaaccaa cgtaaagtaa caacagcaat
    29821 caaacgtgct cgcgttatgg ctcttatgcc ttacgtaaac gaagactaat aaacaaacag
    29881 tccatttcgg actgtttttt agttgactag gactagtcta ggcgtatagg tgagtttttt
    29941 ctaaaagaga gaatgatagt aaacgaaaat ccttgcgtag acagcaagga tgaaatgatt
    30001 gttatttgtt aacaggtgac atagtgtttt gaatgttgta tttgcgttgt aggtaatagc
    30061 gtaaagcaag ggaaatacca ccaataatga gcaaggcaag aggaggcaac tgtgggttaa
    30121 gagaggttgg taaaaaggca gtcgcagagt acaaggcaat ccaaatcaac atggctaatg
    30181 ataaggctgc gatgattttg aaccaacttg gacgaaggct cttatctttt ccgagatgac
    30241 ggtaaataaa gtaataagta gcgtacatgg atgctcctcc accaaaacca agtgccagta
    30301 aggaaatcaa tcctgtaact gtagcgtttg tgttgaagaa ggtcatgatg ccatttaaaa
    30361 gggcaacaat tccgatgaat agcaaagagg tatctagcca catcaaccaa ggattggtgt
    30421 ttttgtcagt ttctgctgct ttttgactag ggtcttggct aaaagaagct gcccaagcag
    30481 tcggagtgcc taatagattt cgagcggtga cacctttttt ctggttttct agaatagctg
    30541 gaatggcttc ttcgagaatc agcttaatgt cttcatcaga cttgccgtct tggattaatt
    30601 tgttcgtagc ggtgtggata aattcttgat tttttttggt aagttcttgt aattccatgg
    30661 gaatcccttc tttatatggg ttagttaaag taagtcaatg atattagcta gtgtcaaatg
    30721 agactttgga gccagccgtt tgacacagag ttttgtcagg tgttaactac attctacagc
    30781 ttgctccact ctagaaccat ttttttctaa taaagtaaat tacaacaaag gaactaagta
    30841 gcatagcaat caaggtaata taccaaaaag catgttctag gccatttagt ggtaaccagt
    30901 tgttttggaa attcatacca taggcggaga aaatcacagt tggtatatct aaagccatgg
    30961 tcatcaaggc cagtgttttc ataatagtgt tctggttgtt gttgataata gaagcagtgg
    31021 tttcagtcat ggcattcaag acattttcat aaatgccagc catctcaatg gcctgttggg
    31081 tttcaatcaa ggtatcttct agcaaatcct cgtcctcaat gtatttcttg agagagcttg
    31141 ttgagctgga taatttcttg acgatgcgtt cgttgaattt caaggaagcc tttaagtaga
    31201 caatggattt ttcaagttcc atcatgtcga taagttcttc atttcgagta gctgcttcaa
    31261 gctgagcttc caaacgttcg ctttggcgat caatggtcct caaggcagtc agaaagagct
    31321 cggcgttgcg atatagaatc tgaaaaacaa aacgtgtctt catgaaggta tagaaatttt
    31381 tgacccggcg gttgtgaaaa tgatcgaaaa gggtcatgtc atgcagacaa gttgtgataa
    31441 cagcattttc cgttacaata atgcctaaag gcatagtaat atagtaactt ttattgttac
    31501 gttcttcgta tatcgggacg tctacgataa ttagggtata atcatcttct acggcaatac
    31561 gtgaggtttc ttccacgtcc aaaggggcgc gcaagtcaga aatatcaatg ttaaattgat
    31621 cggcgatctt catagactct tcttgggaag ggttaaccag tttaatccaa gctcctggct
    31681 cgaatgtttc tatttctttg aattcaattg ccgatgaaag aaacatttgt ttcatgacaa
    31741 gccctccttt ttgatgattg actggtagag attgacatca aaccttgata ataaagcctt
    31801 ctcaaggatt ttctgaaaaa ttctcaatac ttatctatta taccaaaatt cgcaaagttt
    31861 ggggcaatag gttatcagaa atttttgtta taatataaga aagctcgatg aggagggtgc
    31921 ctttaggcac attcaagatg caaaataaaa taataattca tggggcaaga gcccacaatt
    31981 taaaaaatat tgacgtggaa attccacgag acaaattagt tgtcgtgaca gggttatcag
    32041 ggtcaggaaa atctagtcta gcctttgata ctatttatgc ggaagggcaa cgtcgttacg
    32101 tagaaagttt atcagcttat gctagacagt tcttgggtaa tatggaaaaa ccagatgtgg
    32161 attccattga cggtcttagc cctgctattt ccattgatca aaaaactacc agtaagaacc
    32221 cccgctcaac ggttggtacg gttacggaaa tcaatgatta tttacgttta ctatatgcgc
    32281 gtgtgggcac accttactgt attaatgggc atggggccat tacagcatct tctgcagaac
    32341 aaattgtaga gcaggtctta gccctccctg aacggacacg catgcagatt ttagcgccta
    32401 ttgttcgccg gaaaaaaggt caacacaaaa ccatttttga gaaaattcaa aaagatggtt
    32461 atgtccgtgt gcgggttgac ggggatattt ttgacgtgac agaagtgccg gagctgtcca
    32521 aaagtaagat gcacaacatc gaggtggtta ttgaccgtct ggttaataag gatggtattc
    32581 gtagccgttt atttgattcg gttgaagcag ctttaaggct gggagatggc taccttatga
    32641 ttgacactat ggatggcaat gaactgctct tttctgagca ttattcctgt cctgtctgtg
    32701 ggtttacggt tcctgaatta gaaccaagac tcttctcttt taatgcgccg ttcggctctt
    32761 gtccgacctg tgatgggcta ggcattaagt tggaagtgga tttggacttg gttgttcctg
    32821 acccaagtaa gtctttgaga gagggagctt tggcgccttg gaatcctatt tcctccaatt
    32881 attacccaac catgcttgag caagctatgg caagttttgg ggtggatatg gatacacctt
    32941 ttgaggcctt gactgaggaa gaaagagatt tagtgcttta tggttcagga gatagagaat
    33001 ttcattttca ctatgtgaat gattttggtg gtgagcgtaa cattgatatt ccttttgaag
    33061 gtgttgtgac taatgttaat cgtcgttatc acgaaactaa tagtgattat acgcgcaatg
    33121 tgatgcgtgg ttatatgaac gaattgacct gtgcgacttg tcatggttac cgtttgaatg
    33181 accaagcctt gtgtgtgcat gtgggtggcg aagaaggacc tcatattggt caaatttcag
    33241 aattatcgat tgccgaccac ttgcagttgt tggaagaatt ggaactcaca gaaaatgaaa
    33301 gtaccattgc taaaccgatt gtcaaagaaa ttcatgaccg cctgaccttc ttaaataatg
    33361 tgggactgaa ttatctgacc ttgtcacggg ctgctgggac tttatcaggt ggtgaaagtc
    33421 aacgaatcag gttagcgaca caaattggtt ctaacttatc aggggtgatc tatatcttgg
    33481 atgagccttc tattggattg caccaaagag acaatgaccg tttgatcgaa agcctgaaaa
    33541 aaatgcgtga tttgggtaat accttaattg tggtagaaca cgatgaagac accatgatgc
    33601 aggcagactg gctgattgat gtggggcctg gggcaggtga gttcggtgga gaaattattg
    33661 cctctggaac gccgaaacaa gtggctaaga ataaaaaatc catcacgggt caatatttgt
    33721 ctggtaaaaa attcattcca gtgccattag agcgtcgttc tggtaatggg cgattcattg
    33781 aaataaaagg agcagcgcag aataatcttc aaagcctcga cgtgagattc ccactgggta
    33841 aatttattgc ggtcacaggg gtatcaggct cagggaaatc aaccttggtt aatagtattt
    33901 tgaaaaaggc tgtcgcccaa aaattaaacc gtaatgctga taagcctggt aaatatcact
    33961 ctatttctgg tattgaacat attgaacgct tgattgacat tgaccaaagt ccgattggaa
    34021 ggacgccacg gtcaaatcca gccacctata cgggtgtctt tgatgatatt cgagacttgt
    34081 ttgcccaaac caacgaagct aagattcgtg gctacaaaaa aggccgtttc tcttttaatg
    34141 tcaaaggtgg gcgttgtgag gcttgttcag gtgatgggat tatcaaaatc gaaatgcact
    34201 ttttgccaga tgtttatgtg ccttgtgagg tttgccacgg cagacggtat aattctgaga
    34261 cgcttgaggt gcattataaa gaaaaaaata ttgctgaagt acttgacatg actgtcgatg
    34321 atgccttggt atttttctct gctatcccta aaattgcccg aaaaattcaa accattaagg
    34381 atgttggttt gggttatgtg accttgggtc aaccagctac tactctatct ggaggagaag
    34441 cgcagcggat gaaattagct agtgaattgc acaaacgctc gacaggtaag agcttgtata
    34501 tcctagatga gccaacgact ggtttacaca cagatgatat tgcaaggcta ttaaaagttt
    34561 tggaacgctt tgtagacgat ggtaatacgg ttcttgtgat tgagcataat ttggacgtta
    34621 tcaaatctgc tgaccatatt attgacttag gaccagaagg tggtgtcgga ggtggtcaga
    34681 ttgttgccac ggggacacca gaagaggtag ctcaagttaa agaaagctat actggtcatt
    34741 atttgaaagt aaaactacaa caataggatt aggagttcgt ttgagctcct ttttaatctt
    34801 ttaaatgctc tcaaatcatg gtaaacttaa agtagttttt tcatgctaca atattctcaa
    34861 aagttaccaa aggagttcgc gatgtcagga tttttagagc aacgattagg tcactgccta
    34921 aggcagatgg cagagaaggg gctagaggct cttctagtca cccatttaac caatagttat
    34981 tacttgacag gtttttctgg aactgcagca actgttttga taacggccaa acgtcgtgtt
    35041 ttgatcacag attcacgtta taccttgctt gctaaagcta gtgttgaggg atttgatatt
    35101 atcgaaagcc gcacgccgct taaggttgtg gcagaattgt tagaggctga tcaaatagat
    35161 tgccttggtt ttgaggacca ggtatcgttt tctttttatc aggccatgca agcagaactg
    35221 tcaggaataa ccttgcttgc tcagtcaggt tttgtggagc atttacgttt tattaaggat
    35281 acttctgaaa ttgataccat tgctaaagcg tgctcgatct cagacaaagc atttgaagat
    35341 gctcttgatt ttattaaacc agggacaacc actgaacgtg acctggctaa ttttttagat
    35401 tttcgtatgc gtcagtatgg tgccagcggc acatcatttg atatcattgt agcttcaggc
    35461 tatcgctctg ccatgcctca tggacgcgcc agtgacaagg ttatccagaa taaagagagc
    35521 ttgaccatgg actttgggtg ttactacaat cactatgtta gtgatatgac gaggaccatt
    35581 catattggcc aagttactga tgaagaacgt gagatttatg ctcttgttct tgctgctaat
    35641 aaggctctca ttgataaagc tagcgctggt atgacttata gcgattttga cggtattccg
    35701 cgccaactca tcactgaggc tggttatggt agtcacttca cacatggcat tggtcatggt
    35761 atcgggcttg acatccatga gaatccgttt tttggtaaat ctgagcaact tcttcaagca
    35821 ggaatggtgg taacagacga gccaggaatc tatctggata ataaatatgg cgtccgtatt
    35881 gaagatgact tggtcatcac agaaactggc tgtcaagtat tgaccttggc accaaaggaa
    35941 ctgattgtgt tgtaaattat cttagggaag gattagaagg acgaagcaat gacaaatcgt
    36001 ttatcatggc aagattactt tatggccaat gcagagctta tttccaaacg ctctacctgt
    36061 gaccgcgcct ttgtgggagc tgttttggta aaggataatc gtattattgc cacgggttat
    36121 aatggtggtg tttctgccac agataattgt aatgaagcgg gccattatat ggaagatgga
    36181 cattgcatca ggacagttca tgctgaaatg aatgctttga ttcaatgtgc gaaagaaggg
    36241 atttcgactg acggaactga aatttatgta acgcactttc cctgtatcaa ctgtaccaag
    36301 gctctgttgc aggctgggat taaaaagatt acttacaaag cacattatcg tccgcacccg
    36361 tttgcgattg aactaatgga gaaaaaggga gttgcgtacg ttcaacacga cgtgcctcac
    36421 atcattttgg gtggtaagga aggacctaat ggttagcttg ggcgaatggt cataaaagat
    36481 ttgagcctag ggcgcttttg tggtaaaatg aagtgataat attttagtaa gaggtaaata
    36541 ataatgattg aagcaagtaa gcttaaagcc ggtatgacat tcgaatcaga agggaaatta
    36601 attcgtgtcc ttgaagctag ccaccataaa ccaggtaaag gaaacacaat tatgcgtatg
    36661 aaattgcgtg atgtgcgtac agggtctacc tttgacacaa cttaccgtcc agatgaaaaa
    36721 tttgagcaag ctatcattga aactgttcca gcacaatacc tatataaaat ggatgacact
    36781 gcttacttca tgaatactga cacttacgat cagtacgaaa ttccagttgc taacgttgag
    36841 caagagttgc tttacattct tgaaaactca gacgttaaaa tccaattcta tggaagcgaa
    36901 gtgattggag tgactgttcc aacaaccgtt gaattgactg ttgccgaaac acaaccatct
    36961 atcaaaggtg caactgtcac aggttctggt aaacctgcaa ctcttgaaac aggtcttgtg
    37021 gtcaacgttc cagactttat cgaagctggt caaaaactaa tcatcaacac tgcagaaggt
    37081 acttacgttt ctcgtgctta atcctttgtc agaacaggtt agtctaaaga agagaaagga
    37141 aatgctatga caactgaaca tatcggtgaa atcgttattt caccacgagt acttgaggtt
    37201 attactggga ttgctaccac acaagttgag ggcgttcatt cccttcataa caaaaaaatg
    37261 gcagatagct ttaacaaagc aagtcttggt aaaggggttt atctccagac agaagaagat
    37321 ggaagtgtta cagcagatat ttatgtttac ttgcaatatg gagttaaggt cccgactgta
    37381 tctatgaata ttcaaaaaac agtcaaatct gctgtgtacg atatggcaga agtgcctatt
    37441 tctgcagtta atatccatgt tgaaggtatt gttgcggaaa agacaccaaa accagatttg
    37501 aagtcacttt ttgatgagga tttcttggat gactaacagc tttcaaaatt caagaagaga
    37561 tttgcgtgaa cgtgcctttc aggctttatt taacatagag atgggagctg agttattggc
    37621 agcttctcag tttgcctatg gttatgataa agtaacaggg gaggacgctc aagtattgga
    37681 gctgccgatt tttttattaa gtcttgtgac gggtgtcaat aatcataaag aagagttgga
    37741 taacttgata tcaactcatt tgaaaaaagg ttggtcgtta gaacgcttga cactaacaga
    37801 caaaacgtta ttacgactag gtctttttga aatcaagtat tttgacgaaa caccagaccg
    37861 tgttgcacta aacgaaatca ttgaagtagc taaaaaatat tctgatgaaa cctctgctaa
    37921 atttattaat ggcttactta gccaatatgt ctcggaagct ccttcagcaa ataaatcata
    37981 ataagtcgga gcaatcttag gattgtatcc gattttttca atttatgtac gaatattaaa
    38041 agacaaacaa gcatactctc gcatctagaa agagtatgct tgtttgtctt ttagagtaat
    38101 tagtgattgg ttatgttttg tttaaatact tgctcctgga agtagcgtaa tcggtaaaat
    38161 atagtcttta cttgtttttt cttttttaat ttttttgatg agtatttcga cactcaattt
    38221 agcaatctca tcaataggtt gacgaatggt cgctaattga ggaacatatt gctggataaa
    38281 tgtcgttcca tcataaccta tgactttcat atcctctgga atagtaatgt gaagttgttt
    38341 ggctactttc ataatgagga tagcagttaa atcatcagaa acgaaaaggc catcaggttt
    38401 tcgggttgcc aaaatggatt taatttccat ttcgcgtcta acaggagata ggttatttgg
    38461 taatttgatg atttctgcac ttcgtttgag ctggtagtta aaccctagct ggcgcaaccc
    38521 tgtgggcgaa tcagaattgt cattacctgt aatcatgaca atgttttgac aaccgtgttt
    38581 ttgcaaagtt tgtgcggcta atttaccacc ttcaaaatta tctgaagaaa taaccggaat
    38641 atttggcgcc agattacggt caaaagctac aataggagct tcgacacgct cataatcctc
    38701 aattcccaaa ttatgactgg aagaaataat gccgtccact tgattggccg caagcatttc
    38761 taagtattcg cgttctttga cgggattatg ttcactattg caaataatag ttttgtagcc
    38821 ctgtttaaac aattcaattt ccaaatgttc aattaattca gcgtaaaaga tattgctaat
    38881 gttggggaag ataagcccaa tgagttgggt tgatttacct tgcaaacttc tagcgagatt
    38941 gttaggcttg tagcccaatt cacgcatggc cttattcacc ttattcactg ttttttggga
    39001 aaggtagcct ttttggttga ttactcgcga aacagttgtt ggactgacac ccgccaaggc
    39061 tgccacgtct gttaattttg cgaccacttg cctaatacct catttcataa taatgtccaa
    39121 aagcttgtcc ttctactact tggatacctg tttgtccttg ggttgggaaa acacgactag
    39181 ttaggacttt ttgcccttgg ttaataaaga tttcaagaat ggaattgtcc acatagacat
    39241 taagtgtaac atggccttgt gggatttggc aagagcgcac cgtaccatag tcttgggcat
    39301 attgaacccc tgcccgactt cggtcaatgc ttaactgtcc ttttgttgta tccaccttca
    39361 atcttaggcc attccctttt tggtcggcaa ataggagcag gtcaagcttt ttttggcctg
    39421 gaatcgtcaa ttccaattca taacagttag aactgtctat tttattatga aataattctg
    39481 ctggccctcg aagattttgt agggctgaaa caggtgtttg atagagaatt ccgtctttaa
    39541 tccttaattc tttcactaaa ctaagagctc cttgatagtc ataacgatct gtcggataat
    39601 caatatctgg taacccaatc caagacacgg ctagaaccct gccatctgga ctactaaagg
    39661 cttgagtggc ataagcttca aaaccaaagt caagattttg aagggccccc ccacccagca
    39721 attggccagt ttctggattg aagctttcaa aaattttata ggtattgggg tagatatttt
    39781 gataatctaa atcagcttta gccagtcctt gtgggctgaa aatcaaaaca ggcttgtcat
    39841 cgacaaacac aaggttgggg cattcaatca tgtattccgt gccagaatcg tcaaagtcca
    39901 aatcggctat aaatcgccag ttgtccacat gattgtccac agctttgtaa agtttgattt
    39961 ttcctttgcc atctaaacct tgggcgccaa taatagcata gaattgacct tgataagaaa
    40021 aaagctgagg atcccgaaaa tgttctgtaa catcatcggg ctgttcaatc aaaacctgtg
    40081 gtattttgga aatatttcct tgtttatcca tccatgcgcc aacctgtaat ggtgttctga
    40141 cccaattggc atcgcgaaca ttaccagtgt aaaagaggaa gagcttgtca tcaatagcat
    40201 aggcagaacc tgagtaagcc ccatgactat cgtgagcgtg gtctggcaga aggcggctcc
    40261 ttgtttccgt aaaatggacg aggtctgtgg atgtcatgtg tacccattgt ttcagaccat
    40321 gcgcagcccc ataaggccag ttttgataaa aaagatggta gtgaccgttg aaataagaaa
    40381 acccattggg gtcattgagt aatcctgtct taggttcgac atgaaattgg ctgtgccatg
    40441 gggattgggc cattttttca gtaatagctt ggtagtcttt tgagctccac tctttgtaag
    40501 ggcggtagcg aatagcttgt ggtagatcca tcaaaatctc cttgttttat agtcgtcttc
    40561 aagaatatac taaacgtttt ctgtttaaaa atcaagaatt taaagcatat aaaataaaaa
    40621 tatgttaaac gcttgacata aaaaatatta gtgataaaat gatagtcaag aagtgaagcg
    40681 gattcactag cgttggtcat gcacattctt agccacaaag ctggcagaat ggatgaggga
    40741 ctaacaccaa atgaaaaagg agacttttag gcatggataa tcgtcagatt gcaactgaag
    40801 tgattgaggc tttaggtggc cgagaaaatg tgagaagtgt tgcccactgt gcaactcgcc
    40861 ttcgcgtgat ggttcatgat gaaggaaaga ttgataagga aaaagcagaa gctattgaca
    40921 aggttaaagg agctttcttt aattctggtc aatatcaaat gatttttgga actggtaccg
    40981 tcaataacat ttatgacgaa gtcgttgctc ttggtttacc aacatcatca accagtgagc
    41041 aaaaggcaga agcaggcaaa caaggcaata tcttccaacg ggcgattcgt acgtttggag
    41101 atgtttttgt tcccattatt ccagctattg tagcaacggg tctctttatg ggggttcgtg
    41161 gtttggtgac ccagccagct attatggatt tatttggggt gcatgagtac ggggaaaatt
    41221 ttctcatgta cactcgtatt ctaacggata cagcctttgt ctacttgcca gctttggtgg
    41281 cttggtcagc ctttagggtc tttgggggta atcctattat cggtattgtt ttaggtttga
    41341 tgctggtgtc caatgaattg cctaatgctt gggtagttgc ctctggtgga gatgttaagc
    41401 cgctaacctt ctttggattt gttcctgttg ttggttatca aggtaccgtt ttacctgcct
    41461 tctttgttgg tctggtagga gctaagttgg agaaatggtt gcacaaaaag gttccagaag
    41521 ctttagactt attggtcaca ccgtttttaa cgtttgctat tatgagtacc ttgggactat
    41581 ttgtgattgg accagttttc cattctcttg aaaaccttgt tctggctggg acacaggccg
    41641 tcttgcattt gccgtttggc attgctggtc ttattgttgg tggtatccaa cagttgatcg
    41701 tcgttactgg tattcaccat atctttaact tcctagaagc gcagctaatt gccaataccg
    41761 gaaaagatcc tttcaatgct tacctaacag cagcaacagc tgctcaagct ggagctacct
    41821 tagctgttgc tgttaaaacc aaatcaacaa aactaaaagg tctggccttt ccatcaacct
    41881 tgtcagctct tttagggatt actgaaccag ctatttttgg ggtcaacctc cgttatccaa
    41941 aagtctttgt ttcaggtctt attggtggtg ccttaggtgg ttgggtcgct gggctctttg
    42001 gcattgcagg aactggtttt gggattacgg tcttaccagg aactctcctt tacttgaatg
    42061 gccatttatt acaatacctt gtgactatgc ttgtcggtct tggggtagcc tttggaattg
    42121 cttatacttg gggttatcaa gatagagaaa cccttccgtt accagctgtc gaagttgatc
    42181 aaacggctga tcagccagcc ttagcagagg aaactttata tagtcctttg aatggaacag
    42241 ttgtagattt agcagctgtt tcagaccctg ttttttcatc aggtgctatg ggtcaaggct
    42301 tagcaattaa gccagaagat aataccctat actcaccagt tgatggtaaa gttgaaattg
    42361 tctttgaaac aggtcatgcc tatgctataa cgtcaagtca aggagcagaa gtgttacttc
    42421 atattggtat tgacactgtg tcgatggcag gagatggctt tgaatctctt gtagcagtag
    42481 gtcaggcggt taaaaaaggt gatcttcttg gacactttga tcctagcaag attgcagaag
    42541 ctggactaga tgacaccacc atgatgattg tgaccaatag cgctgactat caaagcgtgg
    42601 acattcttgc tcaaggacat gttttgattg gtgatcaagt ggcacttatc aaataaggac
    42661 aaaagagcta actaagcagc tcttttgtcc tgccattcat ggcaggttgg caaaaaaatg
    42721 agaaaagcct aaaaacctta aatttgtgtt attttaccta tactttgtac cttgtttttt
    42781 ttaataaagt gttgttatac ttaaggcgaa ctataggaat gcgcttacat agatggtata
    42841 tcaactggga agccatgact tagtaccaaa aataaggagt gtccaaatgg ataaacattt
    42901 gttggtaaaa agaacactag ggtgtgtttg tgctgcaacg ttgatgggag ctgccttagc
    42961 gacccaccat gattcactca atactgtaaa agcggaggag aagactgttc aggttcagaa
    43021 agaattatct tctatagata gcttgcatta tctgtcagag aatagcaaaa aagaatttaa
    43081 agaagaactc tcaaaagcgg ggcaagaatc tcaaaaggtc aaagagatat tagcaaaagc
    43141 tcagcaggca gataaacaag ctcaagaact tgccaaaatg aaaattcctg agaaaatacc
    43201 gatgaaaccg ttacatggtc ctctctacgg tggttacttt agaacttggc atgacaaaac
    43261 atcagatcca acagaaaaag acaaagttaa ctcgatggga gagcttccta aagaagtaga
    43321 tctagccttt attttccacg attggacaaa agattatagc cttttttgga aagaattggc
    43381 caccaaacat gtgccaaagt taaacaagca agggacacgt gtcattcgta ccattccatg
    43441 gcgtttccta gctggaggtg ataacagtgg tattgcagaa gataccagta aatacccaaa
    43501 tacaccagag ggaaataaag ctttagccaa agctattgtt gatgaatatg tttataaata
    43561 caaccttgat ggcttagatg tggatgttga acatgatagt atcccgaaag taaatggaga
    43621 agctagtgac gaaaatctca agcgatctat tgatgttttt gaagagattg ggaaattaat
    43681 tggaccaaaa ggtgctgata aatcgcgtct gtttattatg gatagcacct acatggctga
    43741 taaaaaccca ttgattgagc gaggagctcc ttatattgat ttgttattgg tacaagtcta
    43801 tggcgcaaga ggagaacaag gtgagtttca aaacgatacc aagttagtta cagatacccc
    43861 tgaagaacga tggcaaggtt atagcaagta tattcgtcct gaacaataca tgattggttt
    43921 ttctttctat gaggaaagag caggcagcgg caatctttgg tatgatatta attctcgcaa
    43981 ggacgaggac aaagcaaatg gaattaacac tgacataact ggaacgcgtg ccgaacggta
    44041 tgcaaggtgg caacctaaaa caggtggggt taagggagga atcttctcct atgctgtgga
    44101 tcgtgatggt gtagctcatc aacccaaaca aatagctgaa aaagataaac agaatgtaaa
    44161 aaataatcaa cctcagattc cagagattac tgataacatt ttccactcag attatagtgt
    44221 ctccaaggca ctaaagacag ttatgctaaa agataagtcg tatgatctga ttgatgagaa
    44281 agatttccca gataaggctt tgcgagaagc tgtgatggcg caggttggaa ccagaaaagg
    44341 tgatttggaa cgtttcaatg gcatattacg attggataat ccagcgattc aaagtttaga
    44401 aggtctaaat aaatttaaaa aattagctca attagacttg attggcttat ctcgcattac
    44461 aaagctcgac caatctgttt tacccgctaa tatgaagcca ggcaaagata ccttggaaac
    44521 agttcttgaa acctataaaa aggataacaa agaagaacct gctactatcc caccagtatc
    44581 tttgaaggtt tctggtttaa ctggtctgaa agaattagat ttgtcaggtt ttgaccgtga
    44641 aaccttggct ggtcttgatg tcgctactct aacgtcttta gaaaaagttg atatttctgg
    44701 caacaaactt gatttggctc caggaacaga aaatcgacaa atttttgata ctatgctatc
    44761 aactgtcagc aatcatgttg gaagcaatga acaaacagtg aaatttgaca agcaaaaacc
    44821 aactgggcat tacccagata cctatggcaa aactagtctg cgcttaccag tggcaaatgg
    44881 aaaagttgat ttgcaaagcc agcttttgtt tgggactgtg acgaatcaag gaaccctaat
    44941 caatagcgaa gcagactata aggcttacca aaatcagcag attgctggac gtagctttgt
    45001 tgattcaaac tatcattaca ataactttaa agtttcttat gagaactata ccgttaaagt
    45061 aactgattcc acattgggaa ccactactga caaaacgcta gcgactgata aagaagagac
    45121 ctataaggtt gacttcttta gcccagcaga taagacaaaa gctgttcata ctgctaaagt
    45181 gattgttggt gacgaaaaaa ccatgatggt taatttggca gaaggcgcaa cagttattgg
    45241 aggaagcgct gatcctgtaa atgcaagaaa ggtatttgat gggcaactgg gcagtgagac
    45301 tgataatatc tctttaggat gggattctaa gcaaagtatt atatttaaat tgaaagaaga
    45361 tggattaata aagtattggc gtttcttcaa tgattcagcc cgaaatccta agacaaccaa
    45421 taaacctatt caggaagcaa gtctgcaaat ttttaatatc aaagattata atctagataa
    45481 tttgttggaa aatcccaata aatttgatga tgaaaaatat tggattactg tagatactta
    45541 cagtgcacaa ggagagagag ctactgcatt cagtaataca ttaaataata ttactagtaa
    45601 atattggcga gttgtctttg atactaaagg agatagatat agttcgccag tagtccctga
    45661 actccaaatt ttaggttatc cgttacctaa cgccgacact atcatgaaaa cagtaactat
    45721 tgctaaaggt ctatctcaac aaaaagataa gttttctcaa aagatgcttg atgagttaaa
    45781 aataaaagag atggctttag aaacttcttt gaacagtaag atttttgatg taactgctat
    45841 taatgctaat gctggagttt tgaaagattg tattgaaaaa aggcagctgc taaaaaaata
    45901 aacaaagcaa ctttcttaga tagcaacatt cagattaaat taacaaaatg tgactatgat
    45961 aaaggtttgc tggaattgat taaccaaaag actaaaaatc tgagatgaat agtcccagat
    46021 ttttagtctt ttataggttt tgatgacata aagctaaata atcgttagac taccagaaag
    46081 tggcacttgt ccgtgagaca tggctgtctt gataagagcc tgtgtcatca taaagataaa
    46141 aaagaacaag aaaaaggaga aaacatggga aaattatatg gaagcattga agctggtggg
    46201 acaaagtttg tctgtgccgt gggtgatgag gagtttacag tggtggataa aacacaattt
    46261 ctgaccacca caccagaaga aaccatcgcg cgaaccatcg cttattttaa agcttttgag
    46321 gcagatttgg ctggaatggc gattggttct tttggaccaa ttgatattga tccgtcgtca
    46381 gagacttacg gtcatatcac aaccactcct aagtcaggct gggctaatgt ggatttgtta
    46441 ggacaacttt cggctgcttt taagattcct tttgatgtga caacagatgt taatagctct
    46501 gcttatggtg aggtgcttgc tcgtcctggt gtggaaagtt tggtctatta caccataggt
    46561 actggtattg gggcgggagc tattcaacat ggccacttta ttgggggatt aggtcataca
    46621 gaagcgggtc atacctatgt catgccccat cctgatgata tggccaaggg atttttaggt
    46681 gtttgccctt ttcataaagg gtgtctggaa gggatggcag caggtccaag tattgaggcc
    46741 agaacgggag tgagaggtga acggcttgat caggaagctg atgtttggga cattcaagcc
    46801 ttctacattg cccaagcagc gctgcaagca actatgcttt accgtcctca agtgattgtt
    46861 tttggcggag gtgtcatgac gcaagaacat atggtgttgc gcgtgcatga taaattcact
    46921 acccttttaa gcggttattt gccagtccct gatttgacag actacattgt tactccagct
    46981 gttgctgata atggctccgc tactctggga aattttgctt tggccaagtt ggctgcacaa
    47041 ggaaaatagg gtctgttttt gcctgcttgt ctagtaaaag aaagcgcagt cagctagaac
    47101 atgctataat aaacagagat taacatcaga agcgaggaat attatgtcag aaccattatt
    47161 tctaaaatca accatgcacg acagaatttg gggtggcacc aagttgagag atgtctttgc
    47221 ttacaacatc ccaagtgaca ccacaggtga gtattgggct atttctgctc atcctaatgg
    47281 tgtttcgaca gttactaatg gacgttatca aggacaacct ttaaatacat tatatgcgca
    47341 agagccggcc ttgtttggga atccaaagga agaagttttt ccactgttaa ctaagatttt
    47401 agatgctaat gactggctaa gtgtccaagt tcatcctgat gatgcttatg gacgtgaaca
    47461 tgaaggagaa ttaggcaaga cagaatgctg gtatattatt tctgccgaag aaggttctga
    47521 aattgtatac ggccatcagg ccaagtccaa agaagacctt cgtgccatga ttgaagcagg
    47581 cgcttgggat gatttgctga ctcgtgtccc agtcaaagca ggcgattttt tctatgttcc
    47641 aagtggcacc atgcatgcga tcggcaaggg aattcttatt ttagaaaccc agcagtcatc
    47701 tgataccact tatcgggttt atgattttga ccgtaaggac gttaatggta atcttcgtga
    47761 ccttcatatt gaaaagtcta ttgatgtctt aacgattggt aagccagaaa atagcgtgcc
    47821 agccactata gttttggata atatggtaga aacaacttta gtctcaacgc catttttcac
    47881 tgtttacaaa tgggtaacta gtcaaatggt tgacatgaaa caagctgcgc cttatctttt
    47941 agtgagtgtt ttaaaaggtc aaggaaaact ctatgtggac cagaaggctt atgagctaga
    48001 aaaaggcatg cactttatct tgcctaacga tgttaaatcg tggtcatttg atggtcaatt
    48061 agaaatgatt gtgagtcacc ccaatcaact cgtttaatac catggtatca atgtgagaaa
    48121 aaaggataaa attatgactc tctgtttctc tgataacttt accaaccttt cttgaactgc
    48181 ctacatattt atgttaaaat gaaatgatta gatgatcgaa aggaaagaga atggccaata
    48241 ttctacgcaa agttatcgaa aatgacaaag gcgaactaag aaaattagaa aaaattgcaa
    48301 aaaaagtaga atcctacgca gaccaaatgg tgtcattgtc ggatagagat ctacaaggaa
    48361 aaacacttga atttaaagaa cgctatcaaa aaggtgagac cctcgaacaa ctattacctg
    48421 aggctttcgc agttgttcgt gaagcagcaa aacgtgtgtt gggcttgttc ccttatcgtg
    48481 ttcaaattat gggaggaatt gttcttcata acggggatgt ccctgaaatg cgtactggtg
    48541 aaggaaaaac tcttacagcc accatgccag tttatctcaa tgctattgct ggtgagggtg
    48601 ttcacgttat tactgttaat gagtatcttt caacgcgtga cgctaccgaa atgggtgaag
    48661 tgtatagctg gttaggtctt tcagttggga tcaacctagc tgctaagtct cctgctgaaa
    48721 aacgtgaagc ttataattgt gatattactt attcaaccaa ctctgaagtc ggttttgatt
    48781 accttcgtga caacatggtt gttcgccagg aagacatggt acaacgccca ctcaattttg
    48841 ccttggttga tgaagtggac tcagtcttaa ttgacgaggc aaggacacct ttaattgtct
    48901 ctggtgctgt tagctctgaa accaatcaat tgtatattcg tgctgacatg tttgtaaaaa
    48961 ctctgacgtc agtggattat gttattgatg tgcccactaa aacgattgga ttaagcgatt
    49021 caggtattga taaagctgag agttatttta acttgagcaa tctttacgat attgaaaatg
    49081 tagccttgac tcactttata gacaacgccc ttcgtgcaaa ctatattatg ttgttagata
    49141 tcgattatgt ggtcagcgag gatggtgaga ttcttattgt tgaccaattt acaggtcgta
    49201 cgatggaagg gcgtcgcttc tcagacggct tgcaccaagc tattgaggca aaagaaggcg
    49261 ttcgtattca ggaagaatca aaaaccagcg cctcaatcac ctaccaaaat atgtttcgta
    49321 tgtacaaaaa attggcaggt atgactggta ctgcgaagac cgaggaagaa gaatttcgcg
    49381 aagtttataa catgcgtatt attccaattc caaccaatcg tccaattgcc cgtattgatc
    49441 acactgattt gctttacccg accttagaat caaagtttag agcagttgtg gaagacgtta
    49501 aaacgcgtca tgcaaaagga caacctatcc ttgtaggtac ggttgctgtt gaaactagtg
    49561 atttgatttc acgcaagctg gttgaagcag ggattcctca tgaggttttg aatgctaaaa
    49621 atcactttaa agaagctcaa atcattatga atgctgggca acgtggtgct gttacaatcg
    49681 caactaacat ggctggtcgt ggtactgata tcaagcttgg tgaaggtgtt cgtgaacttg
    49741 gtgggttatg tgtcattggt acagagcgtc atgaaagccg tcgtatcgat aaccagcttc
    49801 gtggacgttc aggtcgtcaa ggagatccag gtgaatccca attctatctt tcattagaag
    49861 atgatttgat gagacgtttc ggctctgatc gtataaaagc ctttttggat cggatgaaat
    49921 tagatgaaga agacactgtt attaaatcag gtatgttagg gcgtcaagtg gaatctgctc
    49981 aaaaacgggt tgaaggaaat aactacgata cccgtaaaca ggtcttacaa tatgatgatg
    50041 tgatgcgtga acagcgtgaa attatctatg ctaaccgtcg tgatgtcata actgctaatc
    50101 gagatcttgg cccagaaatt aaagctatga tcaagcgtac tatcgaccgt gctgtagatg
    50161 ctcatgcacg tagcaatcgt aaagatgcta tagatgctat tgtaactttt gctaggacct
    50221 cgctggtacc agaagagtct attagtgcta aagagttacg tggtttaaaa gatgatcaaa
    50281 tcaaagagaa gctttatcaa agagcgcttg ctatctacga ccagcaacta tcaaaacttc
    50341 gtgatcaaga agctattatt gaattccaaa aagtactcat tttgatgatt gtggataaca
    50401 aatggactga acacattgat gccttagatc aacttcgtaa tgctgttgga cttcgggggt
    50461 atgctcaaaa taaccctgtg gtggaatacc aagcagaagg tttcaagatg ttccaagata
    50521 tgattggtgc tatcgagttt gatgtaacac gtaccatgat gaaagcacaa attcacgaac
    50581 aagaacgtga acgtgcaagt caacgtgcca caacagcagc acctcaaaac atccaatcgc
    50641 agcagtcagc taacaccgat gatttaccca aagtggagcg taacgaagct tgtccatgtg
    50701 gttcaggcaa aaaattcaaa aattgtcacg gtcgtaaatc cttctcataa gaacgagaca
    50761 caacaggaca aagttaggta tatagaaaga agctgagaat aattctcagc ttctcactgt
    50821 ttaaatcttc taacattaag atgaaataag gtttggtgga aagtaatgat tgttggacat
    50881 ggaatagact tacaagagat ttcagctatt gaaaaagttt atcaaagaaa tcctcgcttt
    50941 gctcaaaaaa ttttaactga gcaggaattg gctatttttg agagttttcc ttataaacgt
    51001 cgactcagct atttggcggg tcgttgggca ggcaaagagg cttttgctaa ggctataggg
    51061 acaggtattg ggcggttaac ctttcaagat attgagattt taaatgatgt tagaggatgt
    51121 cccattctga caaaatctcc gtttaaagga aatagtttta ttagcatctc acatagtggc
    51181 aattatgtac aggctagtgt tattttggag gataaaaaat gatttcaagt ttccatcgcc
    51241 caacagttgc aagggtcaac ttacaagcta ttaaggagaa tgttgccagt gttcaaaagc
    51301 atattccact aggggtaaaa acgtatgcag ttgtcaaggc tgatgcttat ggtcatggtg
    51361 ctgtccaggt gtcaaaagca ctcctacctc aagtggatgg gtactgtgtg tcaaatcttg
    51421 atgaggcttt gcaattacgt caagcaggca ttgataaaga gattttaatt cttggagtcc
    51481 tcctgccaaa tgaattagag ttagcagttg ctaatgctat tactgttaca atcgctagtt
    51541 tagactggat agctttagct agactggaga aaaaagaatg tcaaggctta aaagttcatg
    51601 taaaagttga ttctggtatg gggcggatcg ggcttcgttc ttcaaaagca gtcaatttat
    51661 tgattgatag tctaaaagag ttgggtgctg atgtagaagg tattttcact cattttgcca
    51721 cagctgatga ggcagatgat actaaattta accagcagtt acagtttttt aaaaagctga
    51781 tagctggact tgaggataag cctcgtttag tacatgctag taattcagcc acaagtatct
    51841 ggcatagtga taccattttt aatgctgttc gtttaggaat tgttagttat ggtttgaatc
    51901 caagtggttc tgatctaagc ttaccatttc cactgcaaga ggctttatct ctagaatcta
    51961 gcttagtgca tgtcaagatg atttcagctg gtgatacagt cggttatgga gctacttata
    52021 ctgccaaaaa gtctgaatat gtagggaccg tcccaatcgg ttatgcagat ggctggacca
    52081 ggaacatgca aggcttttcg gtgttagttg atggacaatt ctgtgaaatt atagggcgtg
    52141 tatcgatgga tcaactgacc atacgacttc ccaaagcata tcctttagga acaaaagtca
    52201 ctttgattgg cagcaatcag caaaaaaata tttctacaac agatatcgca aattaccgta
    52261 atacaatcaa ttatgaagtt ctatgccttt taagtgaccg tattcctcgg atatattaag
    52321 aaaatcacgt aaaaataatt gcattgtgct tatctttaat gttataataa agtgaggaca
    52381 ttagaaagaa gtgactaatt aatatatgaa taaaaacaaa ctattaagag ttgccatgct
    52441 actaagtctc ttagccccga cagcagaaag catgacagtg ttggctcaag atgtaatgct
    52501 tgagacgcat aaagcaacta caaatgaaac cagtgattct tcttcaaaag aggaaaataa
    52561 taaaaatgca gcacctacaa catcagataa aactgaccaa ggtccccttg atgcttctgc
    52621 agaaacaaac tctaatagtc ttgttaacgc ggatgataaa aaaagaagcg attctagtca
    52681 gtctgctgta ggctcttcga acaacaaggc agaagcagaa aaccaggtag atgataaatc
    52741 aactgatcat tcgaaaccaa ctgaccagcc aaaaccgact gatcagccca aaccatcacc
    52801 atctaaagtt gatacggcac ctgcttcttc attgtcgaga cagctgccag aggcaagaac
    52861 tcctattcag tcgttgtccc cttacgtatc agatttagat ttgagtgaga tagatatccc
    52921 ttctgtcaac acatacgcgg catatgtaga gcattggagt ggtaaaaatg cctataccca
    52981 tcatctttta tctcgccgtt atggtattaa agctgaccag attgatagtt acttaaaatc
    53041 aacaggcatt gcctatgaca gcacacgtat taatggtgag aagctattgc aatgggaaaa
    53101 gaaaagtggg ctggatgttc gagctatcgt agctattgcg atgtctgaga gttctttagg
    53161 aactcaaggg attgcgactt tgcttggagc taatatgttt ggctatgcag cttttgatct
    53221 agatccgact caagcaagta agtttaatga tgatagtgct attgtcaaaa tgacacaaga
    53281 taccattatt aaaaacaaaa atagcaattt tgcacttcaa gatttaaaag cggctaagtt
    53341 ttcacgaggt caattaaact ttgcaagtga cgggggtgtt tattttactg atactactgg
    53401 tagtggtaaa cgtcgcgcac aaattatgga agacctggat aagtggattg atgaccatgg
    53461 tggcacacca gccattccag ccgaattgaa agtgcagtca tcagctagtt ttgcatctgt
    53521 gccagcaggt tataagctct ctaagagtta tgatgtcttg ggttatcaag cttcgagtta
    53581 tgcttgggga caatgcactt ggtatgtgta taatcgcgcc aaagaattgg gttaccaatt
    53641 tgatcctttt atgggaaatg gtggagattg gaagtataaa gcagggtatg ccctgtcaaa
    53701 gactccaaaa gtaggttatg ctatttcatt tgcaccaggg caagcaggcg ctgatggcac
    53761 ttatggccac gtatcaattg tagaagatgt tagaaaagat gggtctattc ttatttcaga
    53821 gtctaactgt atcggcttag gtaagatttc ttatcgtacc tttacagctc agcaggctga
    53881 acagctaaca tatgttattg gcaagagtaa aaactaataa ctaatgactt ttcttaaggc
    53941 ttaattttct tagcgggagt taggcctttt aaactgttgg tgtaacacga taaagtttca
    54001 agtaaataaa tgattttgag acaatagcac gattagggct attgtctttt tttattgtga
    54061 tgaatatgca gtagtgacat ctcatgttgt ctcctaacac aattatggag cttataacgt
    54121 gttacatgtt ttgtgaatga agtaatgatt gaatcagccc tagtaataac tatcctaagg
    54181 ctagctttat ctcagtaata ttgttgtaga ggaattcttt tatcagagat atcatcatta
    54241 tcaatccagg cttattgcta gtgtcttggc tcacatagtg gagcttttta acagacaatt
    54301 ggaccattgg aaacaatctt gacaaaataa tcacaaagtg atagattaag ttcagttaat
    54361 aaaaaagtta gcttattcat aaaaattttt agaaagttga ttgaaaatga aaaaaatcag
    54421 caaatgtgcg tttgtggcaa tatctgccct tgttctcatt caggctactc aaactgtaaa
    54481 atcacaagag cctttagttc agtcacaact cgtgacaaca gtagctttaa ctcaggataa
    54541 tcgactttta gttgaagaga taggccctta cgctagtcaa tcagctggaa aagagtatta
    54601 taaacatatt gaaaaggtta ttgttgataa tgatgtctat gaaaaaagcc tggaaggcga
    54661 gcgaaccttt gatattaact accaagggat taagatcaat gctaacctta ttaaagacgg
    54721 taagcatgaa ttgactattg ttaataaaaa agatggtgat atcctaatta cctttattaa
    54781 aaagggcgat aaagtgacct ttatttcagc tcaaaaatta ggaacaacag atcatcagga
    54841 ttcattaaaa aaagatgtgc tcagtgataa aacagtgcca caaaaccaag gcacacaaaa
    54901 agttgttaaa tctgggaaaa atactgctaa cttgtcatta ataacaaaat tgagtcaaga
    54961 agatggtgca attttatttc cagaaattga tcgttattct gataacaaac agataaaagc
    55021 attgactcag caaatcacaa aggttacagt caatggtaca gtttataaag atcttatttc
    55081 agattctgta aaagatacta atggctgggt ctcgaatatg acagggcttc atcttggaac
    55141 aaaagctttc aaagatggag aaaatacaat cgtgatatcc tcaaaaggat ttgaagacgt
    55201 taccattacc gttaccaaga aagatggtca aatccatttt gtatctgcca aacaaaaaca
    55261 acatgtgact gctgaagaca gacaatcaac aaagttggat gtcaccactt tgaaaaaagc
    55321 tatcaaagaa gcggatgcga ttattgctaa agaaagcaac aaagacgcgg tcaaagatct
    55381 ggctgagaaa cttcaagtca tcaaggattc ttacaaagaa atcaaagata gtaagctact
    55441 cgccgatact catcgactgt taaaagatac catcgagtct tatcaagcag gtgaggtttc
    55501 tattaacaat ctcacagaag gaacctatac gctaaacttt aaagctaata aagaaaactc
    55561 agaagagtcc tccatgcttc aaggtgcttt tgataaaaga gccaaattag tggttaaagc
    55621 agatggtaca atggaaattt ccatgcttaa tactgcttta ggacaatttt tgattgactt
    55681 ttctattgaa agcaaaggga cctacccagc agcagtgcgt aaacaagttg gccaaaaaga
    55741 tatcaatggt agctatattc gaagcgaatt taccatgcct attgatgatt tggataaatt
    55801 acacaaaggt gctgttttgg tatcagccat gggaggtcaa gaaagtgatt taaaccacta
    55861 tgacaaatac accaaacttg acatgacctt tagtaagacc gttaccaaag gctggagtgg
    55921 ttatcaggta gaaactgatg ataaagaaaa aggggttggg actgaacgtc ttgaaaaagt
    55981 tttagttaaa cttggcaaag atttagacgg cgatggtaaa ttatcaaaaa cggaattaga
    56041 acagattcga ggcgagttgc gtctagacca ttacgagtta actgatattt ctttattgaa
    56101 acatgctaaa aatattacag aactacatct ggatggaaac caaattacgg aaattccaaa
    56161 agagttattt agtcaaatga agcaacttcg atttcttaac ttaagaagta atcatttaac
    56221 ttatctagac aaagatacat ttaaaagcaa cgctcaatta agagaactct acttatcaag
    56281 taactttatt cactctcttg aaggaggact attccagtcg cttcatcacc tggagcaact
    56341 tgatctttcc aagaatcgta ttggccgact ttgtgataac ccatttgaag gattgtctcg
    56401 cctgacttca ttaggtttcg cagaaaatag tcttgaggag atacctgaaa aagcgctaga
    56461 gcctctaaca tcacttaact ttatcgactt atctcaaaat aatttagcac tactgccaaa
    56521 aacaatagaa aaattgcgcg ccttaagcac tattgtggca agtagaaatc atattactcg
    56581 tattgataat atttcattta aaaatcttcc taaattatct gtactcgatt tatcaactaa
    56641 tgaaatttca aatcttccaa atggtatatt taaacagaat aaccaattaa caaaacttga
    56701 ttttttcaat aacttgctta ctcaggttga agaatcagta tttccagatg ttgaaacgct
    56761 taatttagat gtgaagttca atcagataaa aagtgtgagt ccaaaagtaa gagctcttat
    56821 cggacaacac aaactgactc cacaaaaaca tattgcaaaa cttgaagctt ccttagatgg
    56881 cgaaaaaata aaatatcatc aagctttcag tcttttagat ttgtattatt gggagcaaaa
    56941 aacaaattct gccattgata aagaactagt gtctattgaa gaatatcaac aattgttaca
    57001 agaaaaaggt tcagatactg tctctttact taatgatatg caagtcgatt ggagtattgt
    57061 gattcagttg caaaaaaaag cttccaacgg acagtatgtg acggttgacg aaaagcttct
    57121 ctcaaatgat ccgaaagatg acttaacggg agagttttct ttaaaagatc caggtacata
    57181 tcggattcgc aaagctttaa taacaaagaa atttgctact caaaaagaac atatctattt
    57241 gacatctaat gatatccttg tggcgaaagg accacattca catcaaaaag atttagttga
    57301 gaaaggcctt agagcattaa atcaaaaaca attgcgtgat ggtatttact atttaaatgc
    57361 cagcatgtta aaaactgact tagcatctga gtccatgtca aacaaggcta ttaatcatcg
    57421 agtgactttg gtagttaaaa aaggtgtttc ctatttagaa gttgagttta gagggataaa
    57481 ggttggtaaa atgttaggct accttggtga attgagctat ttcgtagatg gttaccaaag
    57541 agatttagct ggtaaaccag ttggtcgaac aaaaaaggca gaggttgtgt cttatttcac
    57601 ggatgtaact ggcctaccat tggcagatcg ttatggaaaa aactatccaa aagtgctgcg
    57661 tatgaaattg attgaacaag cgaagaaaga cggacttgtg ccattacagg tctttgtgcc
    57721 tatcatggat gccatttcaa aagggtctgg ccttcaaacc gtttttatgc gtttagactg
    57781 ggcaagcctt acgacagaga aggcaaaggt tgtcaaagaa actaataatc cacaagaaaa
    57841 tagccatcta acttcaacag atcagttgaa aggacctcaa aatcgtcaac aagaaaaaac
    57901 acctacaagt cctccttcag cagctactgg tattgctaac ttaactgatc tcctggctaa
    57961 aaaagcaacc ggccaatcaa ctcaagaaac ttctaagaca gatgatactg ataaggcaga
    58021 gaaattgaaa cagttagtgc gtgaccatca aacatcaatt gaaggtaaaa cggcaaaaga
    58081 tactaagact aaaaaatctg ataagaaaca tcgttccaat caacaatcaa atggtgaaga
    58141 aagtagctct cgttatcact taattgcagg tctatctagc tttatgatcg tagctctggg
    58201 attcattatt ggtcgaaaga cattatttaa ataagaaact tagtaccaag tgaaataaca
    58261 aaaaagctag tcagcaagag ctaaaaacat acctatttcc gacaggactc ctgatacagg
    58321 ttgaagtagt cgcaagatag gtgtgttttt cacatttgga gatgttataa aatgggaatc
    58381 atttaaaaaa tgaaataaga tgtttaagaa aagatagaca agaggagcat atgacaaagg
    58441 tagtaataaa gcaattgttg caggtaatag tagtgttcat gataagttta tcgacaatga
    58501 caggtgttgt gtatgcagat aaaggtcaaa tttatggatg tattattcag agaaattatc
    58561 gtcatccgat atctgggcag atagaagata gcggaggtga gcattccttt gatattggtc
    58621 aaggtatggt tgaagggact gtctatagtg atgccatgct agaagtatct gatgctggaa
    58681 agatcgttct tacttttcgt atgagcttag ctgattattc gggcaattat cagttctgga
    58741 tacaacctgg aggaactgga agctttcagg cagttgatta caatatcacc caaaaaggta
    58801 ctgataccaa tgggacaaca ctagatattg ctattagttt gcctactgta aatagtatca
    58861 ttcgaggaag catgtttgtt gaacctatgg gcagagaagt ggtcttttac ctgtcagctt
    58921 cagagttaat acaaaagtat tcaggtaata tgttggccca gcttgttact gaaacagaca
    58981 attctcaaaa tcaagaagta aaagacaatc aaaagcctgt tgatacaaaa ctaggagaaa
    59041 gtcaagatga gtctcacaca ggtgctatga taactcagaa taagcctaaa gccaactcat
    59101 caaataacaa gagtctatca gataaaaaaa ttcttccttc taagatgggc ttaacgactt
    59161 ctttagagtt aaaaaaagaa gataagtttc ggtctaaaaa agacttatca atcatgattt
    59221 actactttcc aacttttttt ctgatgttag gagggtttgc tgtatgggtt tggaaaaaga
    59281 ggaaaaaaaa tgataaaacg atgtaaagga attggtctag ccttaatggc cttctttttg
    59341 gtagcttgtg tgaatcagca ccataaaacg gctaaagagt ctgaacagca gagaattgta
    59401 gccacttcgg ttgctgtggt tgacatctgt gaccgtttaa atttagacct cgttggggtt
    59461 tgtgatagta aattatatac ccttcctaaa cgctatgatg ctgttaagcg tgtgggttta
    59521 cccatgaatc ctgatataga gttgattgct tctttgaaac caacttggat tttgagtccc
    59581 aattctttac aagaagattt ggaacccaag tatcaaaaat tggatactga gtatggtttt
    59641 ttgaacttac ggagtgttga gggcatgtac cagtccattg atgatttagg gaaccttttc
    59701 caacgtcaac aagaagcaaa agaattgcgc cagcaatacc aggactatta tcgtgctttc
    59761 caagctaaac gtaaggggaa gaaaaagcct aaagtgctta ttcttatggg cttgccaggt
    59821 agttatttgg tggcgacgaa ccaatcttat gtagggaatc ttttggactt ggcaggtggt
    59881 gagaatgttt atcagtcaga tgagaaagaa tttctatcag ctaatcctga agacatgctg
    59941 gctaaggagc ctgacttgat tttacgaaca gctcatgcca ttccagacaa ggtaaaagtg
    60001 atgtttgaca aagaatttgc tgaaaatgat atttggaaac attttacggc agtcaaggaa
    60061 gggaaagtct atgatttgga caataccctg tttggcatga gtgctaaatt gaactaccca
    60121 gaagccttgg acaccttaac acagcttttt gaccacgtgg gagatcattc gtaacatgac
    60181 agcatcagat ttacaaaggg atcaatccct actggctaaa aaacgtttca gaagtggtct
    60241 ttatctagtt cttttaattc tcagtctcct gtttttggcg gttattgcct tatcccttgg
    60301 tggtttggcg gtttcttatg gggcaattgt caagggactg tttgtggctt atgaccccca
    60361 agtggctttg atttatgatt tgcgctttcc aagaattgtc attgctttat tagcaggagc
    60421 agggatagcg gtttcgggag ttctttttca agcagtgttg aaaaatccta tttcagaccc
    60481 tgctattata gggatttgta gtggtgctag ttttatggtt ttagtctcta gtttacttct
    60541 accgcagttg ctgctgtatg gtccaatcgt atccttttta ggaggcggag tgtctttcct
    60601 gctgatttat ggtttagcat ggaagaaggg gcttaatcct attcggctta ttctaacagg
    60661 gattgctatt aatgctttat ttatggggtt atcaactgct ttaaccagct ttttcacctc
    60721 agctagtccc atggtcaatg ctcttttagc aggtcatatt agtcaaaaaa catgggctga
    60781 tgtaggggtt ttattccctt atacattcat tggcttgtta ctagctttgt tattgtcaaa
    60841 aacctgtaac cttcttcttt tagatgatca ggtgattcgt catttaggaa tcgatgctac
    60901 agcgttacgg ttgggaattt ccttggttgc tgttttattg gcttcggtag ccacttcgat
    60961 cgttggagtg gtttctttcc taggcttgat tgtccctcat atgagccgct tgctggttgg
    61021 aagtaagcac caaatcttaa ttcctttttc agctttgctt ggagcctttg tctttttgct
    61081 agccgatact ttaggaagaa gtcttgctta cccattggaa attagccctg cgattataat
    61141 gagtattgta ggcgggcctt actttattta cctgctaagg aggtctgata ttatatgagt
    61201 aagaacagca gtcatattca ggtgcaaaac ctttcctttg cttatgagga aaaacttgtt
    61261 ttagatgacc tgacctttag cattccaaaa gggaaaatta ccactattat gggggcaaat
    61321 ggcagtggca agtcaacctt attacaatta ttaaccaaaa accttccgtt aaaacaaggg
    61381 caggtttggc ttgagcagga agaattagca acgatttctc tcaaggcttt tgcgaaaaaa
    61441 gtggcagtgg tacaccaata ccatcaagtg gtcgaaggtt tgttagtggg tgaattggta
    61501 gccatggcta ggcttgcccg tcgttctttt tttaaaacta ctaccaaaga agatgaggaa
    61561 cgccttgctt gggccttaga agtaacggct ttgagtgatt atgtttatcg tgacctccaa
    61621 cacctttctg gtggtgaaca gcagagggtt tggattgcca tggcccttgc ccaggataca
    61681 gatattattt ttttagatga gccaacgact tatttggata ttaagtacca gcttgacatt
    61741 ctcaagctga ttactaaaat caaccaagag ctaggcctaa cgattgtgtt agtattacat
    61801 gacatcaatc aggccttgtc tctttcagac catcttattg gactgaaaga tgggcgactt
    61861 tatgcccaag ggcaaccaag agacctcctt gatcaacagt ttatcagaga tgtctttggt
    61921 gttgaattgc cctttgttga gagaaatggt caacagtttg tcctcacttc attggtcaat
    61981 gaaaccaatg atattagaac tcaaaggaga gataggaaca agaacagatg atggaaacag
    62041 aagatacaat atcaagggga aaaaggaaaa gactgttaaa acgattacgt gaacgaattg
    62101 caccaaaaag gtatttgctt tatgtgtcag cctttttatc ttggttacaa tttgtgatga
    62161 ggatgatcag tttttacctc attgccaaaa cgttttcgac ctttattttg ggtcatgcta
    62221 ttgccttagg acgcttggca ggtttactcc tacttttaaa tgtggtaggc tttgttcttg
    62281 ctatattagg caagcaactg caagggatcg cttcacaatt tgctagagat tcactcaaac
    62341 aatctttctt tgaggctttt attgatttag acgggcaatt tgatgctcat gccagtgatg
    62401 ctgatatttt aacgttggct tcacagggga ttgatagttt ggatacttat tatggttact
    62461 atttgtcttt gtctatgaga accaagtgga actgcacaac tattatgata ctagtttttt
    62521 taatttatcc attggcaggc cttgtctttt taggagttct gcttctgatt ccactttcaa
    62581 tcgttgctat gcaaaagcgg tctaagcaaa ttatgagtca ttattggtct tcttatatgg
    62641 atgttggtaa tttatttatg gatgacttaa aaggtcttaa tacactgtat tcttatcaag
    62701 caatagaacg ttacgagcaa gaattttcgg gtaaggcaga acagtttcga aaggctacca
    62761 tgtctttgct tggctttcag ttacaagcag ttggttatat ggatgcagtt atgtatctgg
    62821 gaattggttt gtcaggtttt ttagccgttc aagctttggc aacaggccag ttatcctttt
    62881 ttaatttcct cttttttctc ttgattgcta cagaattttt tacaccgatt cgtgagcagg
    62941 gttatggcat gcacttggtc atgatgaata ccaaaatggc tgaccgtatc tttagtttct
    63001 tggatagtgt tccagcaagg aaagataaca agagtaaaac agcaataaac tttaaccaga
    63061 ttgacatcca aaatatttct ttagcttatg agaaaaaaac agtgcttagt ggggttacca
    63121 tgacattaac gaagggccaa ctaacagcta tcgcgggggt gtcaggacaa ggaaaaacaa
    63181 gtctagctca actcctcttg aaacgccagt ctgcaacgac tgggcatatt ttatttgacg
    63241 gtttagacag tgacaatttg ttgcaagaaa ctatcaacca gcaggttctt tatgtatcgg
    63301 accaatctac cttattgaac cgtagcattt acgataattt aaggttagca gcaaatctct
    63361 ctaaaaaaga aatcttagat tggatagatc aacatggttt gttaagcttc atcaactggc
    63421 taccagatgg tcttgacaca atagttggtg aaaatggtaa cctgttatca ccaggccaaa
    63481 agcaacaggt gatttgcgca agagctctat taagtaagag gtctctttat atctttgatg
    63541 aggcaacatc tagtctagat gcagaaaatg aacgtattat tgacaattta ataacgagat
    63601 tagctaagac agcaatagtt attgtgatta ctcataagat gtctcgactg aaaggagcta
    63661 accaagtact ctttttaaac acagggcagc ctgcttgttt aggcaaacct tgcgaccttt
    63721 atcgggacca accaacttat cgtcacctag ttgatactca agcaagactg gaggcaagta
    63781 tttatggcta aacctaacag atctacctta agcctggttt ggcgtttatt aaccctaatg
    63841 aaaactctgc taccttggat tatcttggct gttggctttg ctgttatggg ttttgtcatc
    63901 acggtaagta ttccaactgg aatagcttat ttaggactac tagctattag acaagaagtg
    63961 attccaatat tagctttata tcttttgatt gctctagcct ttttaagagg ttttgttcgt
    64021 tatggagagc attattttgg tcattttgtt gcttttcata gtttagcagc ttttaggaac
    64081 ttgatcttta agaaactaag agctctttct ccagcttgct tggatagtca agacagtggt
    64141 tatttgctta aaatgattgg agaagatatt gaggcattag aagttttttt tgcccacaca
    64201 atcgcaccta tttgtacagc aatattgtct gcaggtttaa tgttctggta cttttgtcaa
    64261 agtagttggc agttagcctt attagctttg gcaacctatg cttgtttagc cattgttata
    64321 cccatttatt ttgctaatat tttacaggtt ttactaaaaa gccaaaatga aggtcgaaaa
    64381 gattacctgt cttatttttt agagagttta cgatcagtca aagatctttt gcaatttcag
    64441 gtattagacg agcaatttga acgattaata aaaaaaagta atcatgtaaa tgctcttgat
    64501 cgtaacgtag cgcaagccca atttttgcaa atggcattga cttttttttg gcttggctta
    64561 atgatcttag cattttctta tatggttttt gatgggatat gccatgacag cttatcattt
    64621 gataaaggct tattaacttt tattgctttc actgcttcgt tttctccttt tttagaacta
    64681 ggacgtttgc cattaggctt taagcgtgct atgaatgcgg ctcgaaatat ttttgacctt
    64741 ctagacgaaa atgtcatagt agacgaaggc acaaaacaga tagatcgttt aaggagtgtt
    64801 tcttttgagg acctcagttt tgcttatcca aagcgccaag agctggtttt taaggattta
    64861 tcagttacct ttcaagaaaa ggggattatt ggcattaaag gcgaatcagg atctggtaaa
    64921 tcaactttgg tgaaattaat tatgaaatgg tataattgga agacaggaga tatttttctt
    64981 aatgatagga atagttgctt actaaatgct gctaaattac aagatactat tgcttatgtg
    65041 ccacaaacag cgcagctttt ccaacagtct atccgtgaaa atcttacctt tggccgtcaa
    65101 gatatttcag atgagtccat ttggaattta gctgagactt gtggtatgaa agacagactg
    65161 ttagcttgta aggaaggttt ggatacgatt atcaagagtc cttctgactt ttcagctgga
    65221 gaaggtcaac gtttagagtt gatgcgagct ttattgaaag atgcgagttg ctatattttt
    65281 gatgaaccca cgtcaaattt agattctcta aatgaagcta tcttgcttga tttaattaaa
    65341 acacattgtc aaggattggt cttcttaatt tctcacaggc catcaaccct agcttgtgct
    65401 gatcatctat tttgcgtaaa aaatggttcc ctaaaagagg tgaataaaaa atgaaacagt
    65461 taacaataaa agatattatg ataacaggcg catttgccgc gctctacttt atttgtgttg
    65521 gtctaggcac gtttattaac atcttttttg atcgctcagg aaatatgtta tatgcccctg
    65581 cctttgcagc tttactgggg ggaacaattt attttctttt gattgctaaa ttgcaaacat
    65641 ttgggccaat aacattatta ggattgatga tgggaggttt tttcttcctt agtggtcact
    65701 tttttgccgc gatgtttccc agcattattt tcggtttaat agcggatatt attgcttctt
    65761 taggaaaata caagtcaagg ttttttaatg acctatctta tatggttttt gcttttgtca
    65821 atagtggccc tattgtgctg atgtggttgt tgaaagatgc ctatgttgaa agtttgttag
    65881 cccgcggtaa aagtatgacc tatgtcaatc gcattatgta tgattttagt ttagggcatg
    65941 ttatttggtt tgtaggaatt gttcttgcag gagccttatt agggtcagct tttgcacatt
    66001 atttactaaa gaaacatttt atcaaatcag gattgttatc atgaggcttg atgttcgcac
    66061 caaactgctt ttgttggtac ttgccaatgc ctgcttcttt ttccgtgttg atggcttttt
    66121 agaatttata attgttattt ttctcttatt acttttgtct gctttaaata agaaaaagct
    66181 agcattcaag ttggcggttg tttatctgtt aatgatcggt ctatctgtaa tcccgctatc
    66241 tattttccct tcttatcttg atcacttatt gagttttgtc tcaatagcag gaaggttagt
    66301 tttcccttct ttactagcag gtttaattac tattaagaca actaccatct acgaattggt
    66361 tcatggatta cgcaaatggc atttcccaga agtctggcta ttgaccttag ccgttatgtg
    66421 ccgtttcata ccaatgatta gacaagaatg ttgtgttatt catcgctctt taacaatcag
    66481 ggggattatt ttaacaaaat ggtctatttt gatcagacct aaacaatact tggagtattt
    66541 aatggttcca ttgcttttat ctttgataag aagtagccaa gaactaacta tcgctagtct
    66601 aactaaaggg ctagcagtca acaaaggcac cagtgaatgc tttagctcac acttaacatg
    66661 gaaagattgg ggggtgcaga tttggattac tgttatcata atcatcacga tactgcaatg
    66721 atttcagcag aacaacttgt ttttacctat catgatcaaa aaaatccagc ttgtcaggta
    66781 tcgacttgtc aaattgcctc tggacaattc attgttttgt gtggaccttc tggttcaggt
    66841 aaatctacct ttttaaaact tcttaatggc ataattcctg attattatgc tggaaaatat
    66901 gaaggtagac tagatgtggc tgattgccaa gcagacagag acagtgttga aacattttct
    66961 agatctgtgg cctctgtttt tcaaaatcca gccagtcagt ttttctatcg cgaagtccaa
    67021 cacgaactgg tctttccctg tgaaaatcaa ggtttagatg caaaggttat catgaagcgt
    67081 ctttggactt tagcagaaga ttttgctttt gcagagctcc ttaacaagga tatgtttgga
    67141 ttgtcaggag ggcaaaaaca aagagttgct attgcaacgg ccattatgca aggtactaat
    67201 attatgcttt ttgatgagcc aactgctaac ttagatagtg caggcatagc tgctgttaaa
    67261 gcctatctta cccaattaaa agcagcagga aaaaccatta ttgtagccga gcatcgctta
    67321 cattacttga tggatttggc agataacttt ttctacttta aaaatggtag attaacagat
    67381 aagttaacaa cacaagattt gctagccttg acagatgaac aacggcaaga tatgggacta
    67441 cgtcgcttag acttgtctga cctaaaacct gtgttagctg gtaagattga gagtcaacat
    67501 tatcgcccag atgatagcct gtgtattgag catctaacag ttagagcggg aagcaaaata
    67561 cttagatgta ttgaacagct tagttttgct gcaggaagta tttctggcat tacaggatca
    67621 aacgggcttg gaaaatcgca attagtttat tatatagctg gtatcttaga cgataaaaaa
    67681 gcaactatta agtttcaagg cataccttta tctgccaagc aacgtttatc aaagactagt
    67741 attgtcttac aagaagtttc tttgcaacta tttgcagaat cggtttcaaa ggaagtcaac
    67801 ttgggccatg aacgacatcc acggacgaca gaagtaatag agcggttatc tttaacgact
    67861 cttttagaac gtcatccagc tagtttgtca ggaggtgagc aacaaagagt gatgattgcc
    67921 gctagtcttt tggctgataa agatatctta atttttgatg agccaagtag cggacttgat
    67981 ttgttacaaa tgaaggcttt agctaatctt ctcatgcagc tgaagaccca acataaggtt
    68041 gtcatcctca tttcacatga tgaagaactt ctaagtagtg tctgcgatac catttatcaa
    68101 atgctacctt aacgagcccc tagaactgtt taaaagtact aaaaggcttt tatctgaaaa
    68161 taaataggaa ctcttgtata atagatacaa gagtcagaac gtagacaact ccagttttat
    68221 gctcaaaaag catgcatagc tctgctgatt tgacattcgg tttccgattt ttacatataa
    68281 agtgagccaa aacagatgct ttttgctgtg gtaaaagagt ggcgtcaata cgagattgac
    68341 gccactcggg atttttgaga cctcaggctc aaaaataaag gcatggaatt ccaaagaacg
    68401 tcgctgccgt aagcaccacc taaggaaagt atcacgacaa actttgtctt caatctgaca
    68461 agagtttttg tgaagggaaa aagataatga tattaacagc tcctatgtcc aacttaaagg
    68521 gatttggacc aaaatcagca gaaaaatttc agaaattaga tatttataca gtagaagatt
    68581 tactgcttta ttatccgttt cgctatgaag attttaaatc aaaatctgtt tttgatttag
    68641 tggatggtga aaaagcagtc attacaggct tagtcgttac tccagctaat gtacaatatt
    68701 atggttttaa acgtaaccgt ttaagtttca aattgcgtca aggggaagct gtcttaaatg
    68761 ttagtttttt taatcaaccc tatttagctg ataaaataga acttggtcaa gaggtagctg
    68821 tttttggtaa atgggatgcc actaaatcgg ctattactgg gatgaaggtt ttagctcaag
    68881 ttgaagatga catgcaacct gtttatcgcg tagctcaggg aatttcacag tctactttga
    68941 ttaaagctat taagtcagct tttgaaatcg atgcgcattt ggaattgaag gaaaatttac
    69001 cagctacttt attggaaaaa taccgattga tgggtcgtag tcaggcttgt ttagctatgc
    69061 atttcccaaa agatatcaca gagtataagc aagcgctccg tcggattaaa tttgaagaat
    69121 tattttactt tcaaatgaac cttcaagttt tgaaagccga aaatagatct gaaacaaatg
    69181 gtttgcctat tctttatagt aaacatgcta tggagacaaa gatttcctct ttacccttta
    69241 ttctaacaaa tgctcaaaag cgctctttag atgacatatt atctgatatg tcatcgggag
    69301 ctcatatgaa tcgtttattg caaggagatg taggatcagg aaagacagtc attgctggtc
    69361 tatcaatgta tgcagcttat acagcaggtt ttcaatcggc tttgatggtt ccaacggaaa
    69421 tcctagctga acaacactac attagtctgc aagagttatt tccagattta tcaatcgcta
    69481 tattaacttc gggtatgaaa gcagctgtca agcgtacggt tttagcagct attgcaaatg
    69541 gctcggttga tatgattgta ggaactcatg ctcttatcca agactcggta cagtaccata
    69601 aactggggct tgtcattaca gacgagcaac atcgttttgg tgttaaacag cgtagaattt
    69661 tccgtgaaaa gggagaaaat cctgatgttt taatgatgac agccacccca attccccgaa
    69721 ctctagcaat cacagctttt ggagaaatgg atgtttctat tattgatgaa ttacctgccg
    69781 gtcgtaaacc tattatgaca cgctgggtga aacacgagca gctaggtact gtgttggaat
    69841 gggtaaaagg tgaattgcaa aaagatgctc aagtgtatgt catttcaccg ttgattgaag
    69901 aatcagaagc tttagattta aagaatgcag tagcattgca tgctgaatta tctacttatt
    69961 ttgaaggaat tgctaaggtt gctcttgtac atggacgtat gaaaaatgat gaaaaagatg
    70021 ctataatgca agatttcaag gataaaaaaa gtcatatttt agtatccaca acagttattg
    70081 aagtaggggt aaatgtccca aatgcaacga tcatgattat tatggatgcc gatcgttttg
    70141 gattaagtca gttacatcaa cttcgtgggc gtgttggtcg tggatataaa caatcatacg
    70201 ctgttttagt ggctaatccc aaaactgatt cggggaaaaa acgaatgaca atcatgacag
    70261 aaacgacaga tggtttcgtt ttagctgagt cggatttaaa aatgcgtggt tctggtgaaa
    70321 tctttggtac tcgtcagtct ggaattccag aatttcaagt agctgatatc gttgaggatt
    70381 atcctatttt agaagaagca cgcaaagttt ctgcagcgat tgtttctgat cctaactgga
    70441 tatatgaaaa acagtggcaa ttagtggcac aaaatattag aaaaaaagaa gtttatgatt
    70501 aacttaaaac aagtcgttgt tttacaaata tttagttagt tttgagataa actgttataa
    70561 agtataaaga ggtcactatt tctggccttt tttacttatg ctttacgatg agactgctat
    70621 gtgaagcagt attaaccca
//
LOCUS       DATXQK010000006        61510 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.006, whole genome shotgun sequence.
ACCESSION   DATXQK010000006 DATXQK010000000
VERSION     DATXQK010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 61510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61510
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.006"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(116..2023)
                     /locus_tag="VC394_000372"
     CDS             complement(116..2023)
                     /locus_tag="VC394_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEP5993870.1"
                     /translation="MIILQGNKLERSFSGDVLFQNISLQVDERDRIALVGPNGAGKST
                     LLKLLVGEETPTSGEVNTKKDLTLSYLAQNSRFESDKTIYEEMLKVFEALRQDEKRLR
                     QMEMDMATVSGQDLTRLMMDYDLLAERFRQQGGFTYEAEIKAILNGFKFDESMWQMTI
                     AELSGGQNTRLALAKMLLEKPELLVLDEPTNHLDIETIAWLENYLANYQGALIIVSHD
                     RYFLDKVATVTLDLTPHGLDRYVGNYSRFMTLKAEKLVAEEKQFDKQQKEIAKLEDFV
                     QKNIVRASTTKRAQARRKQLEKIERLDKPTSARKSAHMTFQADKPSGNVVLTVEKAAI
                     GYNQHVLSEPINLDVHKLDAIAIVGPNGIGKSTLIKSVIGQIPFIKGEVKYGANVEIG
                     YYDQTQSHLTSSNTVLEELWQDFSTTPEVDIRNRLGAFLFSGDDVKKSVAMLSGGEKA
                     RLLLAKLSMENNNFLVLDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRLAT
                     KVLEITENGSTLYLGDYDYYLEKKAELEELARLAAGETVEETKEASATDYQLQKANQK
                     ERRRLTRRYEEIEARLETIEERIGAIQEDMHASNDTAQLIAWQKEWDQLDQEQETLME
                     EWETIAEQIES"
     gene            complement(2069..2395)
                     /locus_tag="VC394_000373"
     CDS             complement(2069..2395)
                     /locus_tag="VC394_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5993871.1"
                     /translation="MKQLLAIILWLPKLIVKMFWHLIKGFLQTILLVTIIIIGLMYYS
                     NHSDSVLANKISIVTEQVVQIFDILTQKPSAKTRHGSGNSHSTFLVSAKEGNQRLKKS
                     SISFGN"
     gene            complement(2425..3210)
                     /locus_tag="VC394_000374"
     CDS             complement(2425..3210)
                     /locus_tag="VC394_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEP5993872.1"
                     /translation="MASIAIEYHSVVLGMERKVNVIYPDQSEIPKKDQGDKDIPVLYL
                     LHGMGGNENSWQKRTAIERLLRHTNLIVVMPSTDLGWYTDTAYGLNYYRALSQELPQV
                     LAAFFPNMTQKREKTFVAGLSMGGYGAFKWALKSNRFSYAASFSGALDFSPETLLEGN
                     LGELAYWQGVFGQFDDPDLDKHYLKNMVAESDGKTKFYAWCGYEDFLFATNEKAIADF
                     QAQGLDIDYHKGHGKHEWYYWNQQLEVLLEWLPINYQKEERLS"
     gene            complement(3343..5004)
                     /locus_tag="VC394_000375"
     CDS             complement(3343..5004)
                     /locus_tag="VC394_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEP5993873.1"
                     /translation="MTDIKMIALGGVREYGKNFYLVEINDSMFILDAGLKYPENEQLG
                     VDLVIPNLDYVIENKGKVQGIFLSHGHADAIGALPYLLAEVSAPVFGSELTIELAKLF
                     VKSNNSTKKFNNFHVVDSDTEIEFKDGLVSFFRTTHSIPESMGIVIGTDKGNIVYTGD
                     FKFDQAAREGYQTDLLRLAEIGKEGVLALLSDSVNATSNDQIASESEVGEEMDSVISD
                     ADGRVIVAAVASNLVRIQQVFDSATAHGRRVVLTGTDAENIVRTALRLEKLMITDERL
                     LIKPKDMSKFEDHELIILEAGRMGEPINSLQKMAAGRHRYVQIKEGDLVYIVTTPSTA
                     KEAMVARVENLIYKAGGSVKLITQNLRVSGHANGRDLQLLMNLLKPQYLFPVQGEYRD
                     LAAHAKLAEEVGIFPENIHILKRGDIMVLNDEGFLHEGGVPASDVMIDGNAIGDVGNI
                     VLRDRKVLSEDGIFIVAITVSKKEKRIISKAKVNTRGFVYVKKSHDILRESAELVNTT
                     VGNYLKKDTFDWGELKGNVRDDLSKFLFEQTKRRPAILPVVMEVR"
     gene            complement(5208..5966)
                     /locus_tag="VC394_000376"
     CDS             complement(5208..5966)
                     /locus_tag="VC394_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5993874.1"
                     /translation="MTKIIELINATVDVDNGFEDAKTILDNVTLTIYEHDFLTILGGN
                     GAGKSTLFNVIAGTLSLTRGQIRILGQDVTHWPAEKRALYLSRVFQDPKMGTAPRMTV
                     AENLLIARQRGEKRSLASRKITEHLASFEDLVKRTGNGLEKHLETPAGLLSGGQRQAL
                     SLLMATLKKPALLLLDEHTAALDPKTSQSLMQLTDEFVTKDGLTALMITHHMEDALTY
                     GNRLIVMKDGNIIKDLNQMEKEQLTITDYYQLFD"
     gene            complement(5963..6832)
                     /locus_tag="VC394_000377"
     CDS             complement(5963..6832)
                     /locus_tag="VC394_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP5993875.1"
                     /translation="MIISSVSQGLIWGVLGLGIYLTFRILNFPDMTTEGSFPLGGAVA
                     VTAISLGWNPFLSTLLGMLSGALAGFLTGLLYTKGKMPTLLAGILVMTSCNSIMLMVM
                     GRANLGLHDHKRIQDCLPFSIDLNSLLTGLITVVIVISVLIYFLYTNLGQAYIATGDN
                     KDMAKSFGINTDWMEVMGLVVSNSLIALSGALVSQQDGYADVSKGIGVIVIGLASIIV
                     GEVLYSTGLTLLERLIAIVIGSILYQFLISVVITLGFNTSYLKLISALVLALCLMIPV
                     VKERYFKGVRLTR"
     gene            complement(7177..8175)
                     /locus_tag="VC394_000378"
     CDS             complement(7177..8175)
                     /locus_tag="VC394_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEP5993876.1"
                     /translation="MKNKSLIATLLVLTVIVIGSLLSKGVSKENRDLANRQNITIGIL
                     QFVTHEALDDIKRGIEDQLKKQMPQKQNVVIKVMNAEGDQSKIQTMSRQLVQSGSDIV
                     IGIATPAAQGLAAASKDIPVVMSAVSDPVGSRLVMQLDQPEANVTGLSNKVPVKQTID
                     LMKNLTPHVKTVGILYASNEDNSLSQVKEFRRLARKKGYQVISYAVPSTNEVPATMSV
                     MLGKVDAVFIPQDNTIASAFSSVMTTSKAAKIPVYTSVDRMVEKGGLAAISQNQYDLG
                     VQTANQVLKLIKGKRVVDVPVKVVDIGQPLINKNVAAELGIAINREDFPSASFIEN"
     gene            8529..10181
                     /gene="fbp54"
                     /locus_tag="VC394_000379"
     CDS             8529..10181
                     /gene="fbp54"
                     /locus_tag="VC394_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein Fbp54"
                     /protein_id="HEP5993877.1"
                     /translation="MSFDGFFLHHLTNELKENLLYGRIQKVNQPFERELVLTIRNHRK
                     NYKLLLSAHPVFGRVQITQADFQNPQVPNTFTMIMRKYLQGAVIEQLEQIDNDRIIEI
                     KVSNKNEIGDAIQATLIIEIMGKHSNIILVDRAENKIIESIKHVGFSQNSYRTILPGS
                     TYIEPPKTAAVNPFTITDVPLFEILQTQELTVKSLQQHFQGLGRDTAKELAELLTTDK
                     LKRFREFFARPTQANLTTASFAPVLFSDSHATFETLSEMLDHFYQDKAERDRINQQAS
                     DLIHRVQTELDKNRNKLSKQEAELLATENAELFRQKGELLTTYLSLVPNNQDSVILDN
                     YYTGEKIEIALDKALTPNQNAQRYFKKYQKLKEAVKHLSGLIADTKQSITYFESVDYN
                     LSQASIDDIEDIREELYQAGFLKSRQRDKRHKRKKPEQYLASDGKTILMVGRNNLQNE
                     ELTFKMAKKGELWFHAKDIPGSHVIIKDNLDPSDEVKTDAAELAAYYSKARLSNLVQV
                     DMIEAKKLHKPSGAKPGFVTYTGQKTLRVTPDQAKILSMKLS"
     gene            complement(10240..11487)
                     /locus_tag="VC394_000380"
     CDS             complement(10240..11487)
                     /locus_tag="VC394_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEP5993878.1"
                     /translation="MLNSEKMIASLDQQDLAHAEKYFQKALKEDDADSLIALGEYLES
                     IGFLPHAKRIYLQLADDYPELNINLAQIAAEDDAIEEAFLYLDKVSKDSPNYLSALLV
                     MADLYDMEGLTEVAREKLLQAVSISPEPLVIFGLAEIDMSLQYFKEAIDYYAQLDNRQ
                     ILELTGISTYQRIGRAYASLGKFEAAIEFLEKAVAIEYEDETVFELATLLYDQENYQK
                     ANLYFKQLETINPDYPGYEYGYALSLHEEHKTSEALRLVQQGLRKNAFDSQLLLLASQ
                     LSYELHDRQNAENYLLQAKEVAVDDEEILMRLVTLYFDAERFEEVIALNRETIDNVLT
                     KWTIAKAYHALEQEEVALALYNEISADLAENPEFLQDYAYLLREFGQFHKAIQMATAY
                     LRQVPDDVNMQDFLDHIKHQQSE"
     gene            complement(11477..12658)
                     /locus_tag="VC394_000381"
     CDS             complement(11477..12658)
                     /locus_tag="VC394_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEP5993879.1"
                     /translation="MPLIPDKSRTDSLFYKWFLNNQATMALVITLLAFLTIFVFTKIS
                     FLFMPVISFFAVIMLPLVISTILYYLTKPLVDLINHLGPNRTTSIFIVFGLITLLFVW
                     AISGFVPMVQTQLTSFIEDLPKYVGKVNEEANKLLENEWLVSYKPQLQDMLTHTSQKA
                     LDYAQSFSKNAIDWAGNFAGAIARITVAIIISPFILFYFLRDSSHMKNGLVNVLPLKL
                     RVPMVRVLGDINKQLSGYVQGQVTVAIVVGFMFSIMFSLVGLKYAITFGIIAGFLNMI
                     PYLGSFLAMIPVVIMAMVQGPFMLVKVLVIFMIEQTIEGRFVAPLVLGNKLSIHPITI
                     MFLLLTAGSMFGVWGVFLVIPIYASVKVVIKELFDWYKKVSGLYDEEVLVIEEVKDHV
                     K"
     gene            complement(12716..13192)
                     /locus_tag="VC394_000382"
     CDS             complement(12716..13192)
                     /locus_tag="VC394_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEP5993880.1"
                     /translation="MTQLATICYIDNGDSLLLLHRNKKENDVHKGKWISVGGKLEAGE
                     TPDECARREILEETHLTVTEMAFKGIITFPEFTPGHDWYTYVFKVTGFEGDLISDEES
                     REGTLEWVPYDQVLEKPTWEGDYDIFKWILEDRPFFSAKFTYDQNNQLMDKSVTFY"
     gene            complement(13413..14453)
                     /gene="rfbB"
                     /locus_tag="VC394_000383"
     CDS             complement(13413..14453)
                     /gene="rfbB"
                     /locus_tag="VC394_000383"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEP5993881.1"
                     /translation="MYKNIIVTGGAGFIGSNFVHYVYNNHPDVHVTVLDKLTYAGNRA
                     NIEAILGDRVELVVGDIADAELVDKLAAKTDAIVHYAAESHNDNSLEDPSPFIHTNFI
                     GTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGQGEGPGEKFTAETKYNPSSPYS
                     STKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILSGIKPKLYGE
                     GKNVRDWIHTNDHSTGVWAILTKGRIGETYLIGADGEKNNKEVLELILEKMGQPKDAY
                     DHVTDRAGHDLRYAIDSTKLREELGWEPQFTNFSEGLEETIKWYTENETWWKAEKDAV
                     EAKYAKTQEVIK"
     gene            complement(14697..15290)
                     /locus_tag="VC394_000384"
     CDS             complement(14697..15290)
                     /locus_tag="VC394_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEP5993882.1"
                     /translation="MTETFFDKPLACREIKEIPGLLEFDIPVRGDNRGWFKENFQKEK
                     MLPIGFPERFFEEGKLQNNVSFSRQHVLRGLHAEPWDKYISVADDGKVLGAWVDLREG
                     ETFGNVYQTVIDASKGMFVPRGVANGFQVLSETVSYSYLVNDYWALDLKPKYAFVNYA
                     DPSLGITWENLAAAEVSEADKNHPLLSDVKPLKPKDL"
     gene            complement(15290..16159)
                     /gene="rfbA"
                     /locus_tag="VC394_000385"
     CDS             complement(15290..16159)
                     /gene="rfbA"
                     /locus_tag="VC394_000385"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEP5993883.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPIYDKPMIYYPLSTLMLA
                     GIKDILIISTPQDLPRFEELLGDGSEFGISLSYKEQPSPDGLAQAFIIGEEFIGDDRV
                     ALILGDNIYHGNGLTKMLQKAAAKEKGATVFGYQVKDPERFGVVEFDENMNAISIEEK
                     PEVPKSHFAVTGLYFYDNDVVEIAKNIKPSARGELEITDVNKAYLERGDLSVELMGRG
                     FAWLDTGTHESLLEAAQYIETVQRLQNAQVANLEEIAYRMGYISKEDVHELAQSLKKN
                     EYGQYLLRLIGEA"
     gene            complement(16218..17324)
                     /locus_tag="VC394_000386"
     CDS             complement(16218..17324)
                     /locus_tag="VC394_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEP5993884.1"
                     /translation="MKIAIIGAGIVGSTAAYYLQQSGQKEVTIFDHGQGQATKAAAGI
                     ISPWFSKRRNKVWYRMARLGADFYQQLINDLKEDGFATDFYQQNGIYVLKKQEEKLRD
                     LYELALARKVESPIIGELAIKNRKELGDDFKGLIGFDNCLYASGAARVEGAALCETLL
                     KTSGYPVIRQKVTLKQQGSGYEIAGHYFDQVILAAGAWLPDLLMPLGYQVDVRPQKGQ
                     LLDYDVHHIISDNYPVVMPEGEIDLIPFNQGKISVGASHENDKGYDLEPDWQVLEKLE
                     MQALTYLPLLKEATQKSCRVGIRAYTSDYSPFYGQVPGLKNLYTASGLGSSGLTVGPL
                     IGYELAQLLLGHEGLLTPSDYSPEPYLLRSTGKA"
     gene            complement(17364..18152)
                     /locus_tag="VC394_000387"
     CDS             complement(17364..18152)
                     /locus_tag="VC394_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019781659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEP5993885.1"
                     /translation="MKAKTLIDAYEAFCPLDLSMERDVKGLQMGSLDKDIRKVMITLD
                     IRESTVAEAIKNEVDLIITKHAPIFKPLKDLVSSPQRDILLDLVKHDISVYVSHTNID
                     IVPGGLNDWFCDLLEIKEATYLSETKEGFGIGRIGTVKEQALEELASKVKRVFDLDTV
                     RLIRYDKENPLISKIAICGGSGGEFYQDAVQKGADVYITGDIYYHTAQEMLTEGLFAV
                     DPGHHIEVLFTEKLKEKLQGWKEENGWDVSIISSKASTNPFSHL"
     gene            complement(18142..18828)
                     /locus_tag="VC394_000388"
     CDS             complement(18142..18828)
                     /locus_tag="VC394_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEP5993886.1"
                     /translation="MDSQLSNRLAQVAAYVPKGVKLLDVGSDHAYLPIFLVETNQISA
                     AIAGEVVRGPYESALKNVTQSGLAEHIQVRLANGLAAFEEADDVTAITICGMGGRLIT
                     DILEAGKEKLQGIERLVLQPNNREDDLRAWLSVNAFKIVAETIMAENDKYYEIIVAEH
                     GEKALSATELRFGPYLSQEKSVVFKEKWQREMDKLAYALSCIPEEKTQERQLLLTKIQ
                     QIKEVIVHES"
     gene            complement(18900..19556)
                     /gene="nth"
                     /locus_tag="VC394_000389"
     CDS             complement(18900..19556)
                     /gene="nth"
                     /locus_tag="VC394_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEP5993887.1"
                     /translation="MRIGKARLAKVLTIIGQMFPEAKGELDWETPFQLLIAVILSAQT
                     TDKAVNKVTPGLWQSYPEIEDLAFAELSDVENALRTIGLYKNKAKNIIKTAQAIRDDF
                     KGQVPKTHKELESLPGVGRKTANVVLAEVYGVPAIAVDTHVARVSKRLNISSPDADVK
                     QIEADLMAKIPKKDWIITHHRLIFFGRYHCLAKKPKCEICPVQSYCKYYQNTYGKSKA
                     "
     gene            complement(19553..20236)
                     /locus_tag="VC394_000390"
     CDS             complement(19553..20236)
                     /locus_tag="VC394_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEP5993888.1"
                     /translation="MSFLEHYKSGNLVIPSALLFHYKDLFKSSDDFLVWQFFYLQNTT
                     KRDDLAPSQIAHALGKSVADINKIISSLTSQGLLDMRTIELTGEIEIIFDASPVLAKL
                     DQLFVSQTATEIDKQETPNHFKRLVDEFERELGRFLSPFELEDLEKTLRDDKTDPDLI
                     REALKEAVFNGKTNWKYIQAILRNWRKEGIVNLRQVEERRRVREGEDLSQVTISEDFL
                     SAMNLWSDS"
     gene            complement(20317..20835)
                     /locus_tag="VC394_000391"
     CDS             complement(20317..20835)
                     /locus_tag="VC394_000391"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEP5993889.1"
                     /translation="MDLTNYIASIKDYPKAGITFRDISPLMADGKAYSYAIREIAQYA
                     CDKDIDMVVGPEARGFIIGCPVAVELGIGFAPVRKPGKLPRDVVSADYEKEYGLDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGIVAGCAFLIELEGLNGRHAIR
                     NYDYKVLMQFPG"
     gene            complement(20985..23195)
                     /gene="recJ"
                     /locus_tag="VC394_000392"
     CDS             complement(20985..23195)
                     /gene="recJ"
                     /locus_tag="VC394_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEP5993890.1"
                     /translation="MIKSKYSWKIKDKKPDDGFFKLAKTKGLTQTAAQLIYERGIRTE
                     EALDEFLTADLSQLHDPYLLHDMAKAVPRIRQAIEEGERILIYGDYDADGMTSASIVK
                     ETLDMMGAEPLVYLPNRFTDGYGPNQSVYKYFIEQEAVSLIITVDNGVAGHEAIRYAQ
                     EQEVDVIVTDHHSLPEELPEAFAIIHPEHPDADYPFKHLAGCGVAFKLATALLESLPT
                     DCLDLVAIGTIADMVSLTGENRVLVKNGLAMLKHTERVGLQELMSLSPIDLEHFNEDA
                     IGFQIAPQLNALGRLDDPNPAIELLTGFDDQEAQAIALMIKKKNEERKALVQDIFDQA
                     MAMVDPQKPVQVLAQAGWHPGVLGIVAGRIMETIGQTVVVLTIDNGFAKGSARSLEAI
                     NIFEALNGKRELFTAFGGHAGAAGMTLPVDNLEALSDFLCQFVIERGLDQTAKNTLTI
                     DERLSLDDLSLDILKSLDKLAPYGMDHQKPVFYVKDIRVSQARTIGQDQSHLKFKVSQ
                     GKASFDVLAFGQGSQLQEFRQATGLELAVTLSVNHWNGNTSLQFMLVDARVDGVQLLD
                     LRTKTAKVPEGIPTIEEDPNARVILINDIPEDFKTWRNQFVHKDFDAIYFKNQMKHPY
                     YLTGFGSREQFAKLYKTIYQFPEFDLRHKLTELSHYLNIEKLLLIKLIQIFEELSFVT
                     IDDGLMTVNPQAQKREISESHIYQDLKELVKFQEIMALASPKEMYDYLVKSNDQ"
     gene            complement(23192..23956)
                     /locus_tag="VC394_000393"
     CDS             complement(23192..23956)
                     /locus_tag="VC394_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEP5993891.1"
                     /translation="MAQRIIVITGASGGLAQAIVKQLPKEDSLILLGRNKERLEHCYQ
                     HIDNKECLELDITNPVAIEKMVAQIYQRYGRIDVLINNAGYGAFKGFEEFSAQEIADM
                     FQVNTLASIHFACLIGQKMAEQGQGHLINIVSMAGLIASAKSSIYSATKFALIGFSNA
                     LRLELADKGVYVTTVNPGPIATKFFDQADPSGHYLESVGTFTLQPNQVAKRLVSIIGK
                     NKRELNLPFSLAVTHQFYTLFPKLSDYLARKVFNYK"
     gene            complement(23956..24885)
                     /locus_tag="VC394_000394"
     CDS             complement(23956..24885)
                     /locus_tag="VC394_000394"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEP5993892.1"
                     /translation="MELQFLGTGAGQPAKQRNVSSLALKLLDEINEVWMFDCGEGTQR
                     QILETTIKPRKIRKIFITHLHGDHIFGLPGFLSSRSFQASEEQTDLDIYGPIGIKTYV
                     LTSLKVSGARVPYQIHFHEFDDKSLGKIMETDKFVVYAERLAHTIFCMGYRVVQKDLE
                     GTLDAEALKAAGVPFGPLFGKIKNGQDVELEDGRLICAKDYISAPKKGKIITIIGDTR
                     KTSASVKLAKDADVLVHESTYGKGDERIARNHGHSTNMQAAQIAHEAGAKRLLLNHVS
                     ARFLGRDCRQMEKDAATIFENVKMVQDLEEVII"
     gene            complement(24900..25535)
                     /locus_tag="VC394_000395"
     CDS             complement(24900..25535)
                     /locus_tag="VC394_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEP5993893.1"
                     /translation="MNNYIDLAKTYGGFTSLDTNYLNHLLASLTDQQKLAFITPPPSV
                     INAYFAEIYQKQSPQAATDYYFNLSKALGLFTDQPSFEEEKPFVRLNLSGKAYGFAYQ
                     NDQEVALVFSEKAEPKKPELFFELTQIFPQYMVYEDKGQLKMQAKQFEQGECEDITPD
                     DTLLSKIYRLANGITMLKGFNVEELWALSQTFSGQKYYDFAQREFMIYITQ"
     gene            complement(25528..26766)
                     /gene="hflX"
                     /locus_tag="VC394_000396"
     CDS             complement(25528..26766)
                     /gene="hflX"
                     /locus_tag="VC394_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEP5993894.1"
                     /translation="MIETKRQQERVILLGVELQTTEHFDMSMTELANLAKTAGVKVMA
                     SFSQKRERYDSKTFIGSGKLDEIKAIVEADEIDAVIVNNRLTARQNANLEAVLEVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYMLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSIRHQIADIERQLTQVEKNRQTIRDRRVGSDTFKIGLIGYTNAGKSTIMNLL
                     TDDSHYEANELFATLDATTKQLYLENQFQATLTDTVGFIQDLPTELVAAFKSTLEESK
                     YVDLLLHVIDASDPNHSEQEKVVLNLLKELDMLNIPRLAIYNKVDIAEQFTATAFPNI
                     RISARSKDSKILLRRLIIDQIRDQFVPFRIKVHQDKAYKLYDLNRVALLDHYTFDQEI
                     EDISGYISPKQQWRLDDFYE"
     gene            complement(26839..27738)
                     /gene="miaA"
                     /locus_tag="VC394_000397"
     CDS             complement(26839..27738)
                     /gene="miaA"
                     /locus_tag="VC394_000397"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEP5993895.1"
                     /translation="MTKIKIVVIVGPTAVGKTALGISLAKAFNGEIISGDSQQVYRQL
                     DIGTAKATQEEQEAAVHHLTDIREVTESYSAYDFVQDAQKAISDIVSRGKLPIIVGGT
                     GLYLQSLLEGYHLGGQVDQEAVKAYRNELEQLDDHDLYERLQVNNITIEQVNRRRAIR
                     ALELAQFADELENAETAYEPLIIGLNDDRQVIYDRINQRVDRMLENGLLEEAKWLYEH
                     YPTVQASRGIGYKELFPYFVGEMTLAEASDQLKQNTRRFAKRQLTWFRNRMAVSFTAI
                     TAPDYPQVVHDRVRDFLGQKEKS"
     gene            27866..28042
                     /locus_tag="VC394_000398"
     CDS             27866..28042
                     /locus_tag="VC394_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002263068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEP5993896.1"
                     /translation="MIMEYQFVKGLATGVLATAATVAGAVFAVKKTIIDPEEEKAAFI
                     EENRKKAARRRVAR"
     gene            complement(28160..29068)
                     /locus_tag="VC394_000399"
     CDS             complement(28160..29068)
                     /locus_tag="VC394_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEP5993897.1"
                     /translation="MKHLKNPPLVMSGLALGTLSFGNLLATYVSIFSYLGILAALFIY
                     GILLVGMVRNLNDTKMQLRQPLIASVFPTFFMTGMLLSSLFLKVTGGCWLGFLTWWLF
                     FLGNLVLIAYYQYRFVFSFSWDNVFPSWSVLFVGIAMAALTAPASRQFLLGQVIFWVC
                     LLLTAVILPFMAKKTYGIGLGQAVMPNISTFCAPLSLLSASYLATFPRPQVGMVIFLL
                     ISSQLLYAFVVVQLPRLLNRPFNPGFSAFTFPFVISATSLKMTLSFLGWQGLGWQVLL
                     LGEVLLATALVTYVYGAYLRFLFQNK"
     gene            complement(29127..29513)
                     /locus_tag="VC394_000400"
     CDS             complement(29127..29513)
                     /locus_tag="VC394_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEP5993898.1"
                     /translation="MLFIAKQTLKSQLIPQDNLLAESRFNEIMDFLTGDFPLVFRPMY
                     NPHRYTISQDNQALEKVKQASYKRMDIAMTHLDGLIGDSGHVYRDQQTIADAYAYAMA
                     LWSQKTPKSYENYPHLAAFMAKMLEM"
     gene            complement(29824..30810)
                     /locus_tag="VC394_000401"
     CDS             complement(29824..30810)
                     /locus_tag="VC394_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEP5993899.1"
                     /translation="MSEKIRVLLYYKYVSIENAQEYAAKHLEFCKSIGLKGRILIADE
                     GINGTVSGDYETTQKYMDWVHSDERFADLWFKIDEENQQAFRKMFVRYKKEIVHLGLE
                     DNNFDSDINPLETTGEYLNPKQFKEALLDEDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKDKFMEKRVVVYCTGGVRCEKFSGWMVREGFKDVGQLHGGIATYGKD
                     PEVQGELWDGAMYVFDDRISVPINHVNPTVISKDYFDGTPCERYVNCANPFCNKQIFA
                     SEENETKYVRGCSPECRAHERNRYVQENGLSRQGWAERLEAIGESLPEFVGA"
     gene            31010..31312
                     /locus_tag="VC394_000402"
     CDS             31010..31312
                     /locus_tag="VC394_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5993900.1"
                     /translation="MTYELCLEYGTYPLSRVYAYWGEDQNPPTFIQEDRLLCHKLETM
                     NHLFHDLFVTIESQFHYVGFNMPEKRAQIRILYQEVATILKSKYKDYPIKIETFLL"
     gene            complement(31675..31746)
                     /locus_tag="VC394_000403"
     tRNA            complement(31675..31746)
                     /locus_tag="VC394_000403"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31711..31713),aa:Arg,seq:ccg)
     gene            complement(31859..33064)
                     /gene="rpsA"
                     /locus_tag="VC394_000404"
     CDS             complement(31859..33064)
                     /gene="rpsA"
                     /locus_tag="VC394_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002997242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEP5993901.1"
                     /translation="MNEFEDLLNSVSEVNPGDVVTAEVLTVDNGQANVVIEGTGVEGV
                     LTLRELTNDRDADINDFVKAGDTVEVLVLRQVVGKDTDTVTFLVSKKRLEARKAWDKL
                     VGREGEVVTVKGTRAVKGGLSVEFEGLRGFIPASMIDTRFVRNTEKFVGQEFDAKIKE
                     VDAAENRFILSRREVIEEAAKEARAEVFSKISEGAVVTGTVARLTSFGAFIDLGGVDG
                     LVHVTELSHERNVSPKSVVSVGEEVEVKVLSIDEEAGRVSLSLKATTPGPWDGVEQKL
                     AQGDVVEGKVKRLTDFGAFVEVLPGIDGLVHISQISHKRVENPKDVLSVGQEVTVKVL
                     EVNAADERVSLSIKALEERPAQAEGDNKEEKRQSRPRRPKRESRRDYELPETQTGFSM
                     ADLFGDIEL"
     gene            complement(33191..33262)
                     /locus_tag="VC394_000405"
     tRNA            complement(33191..33262)
                     /locus_tag="VC394_000405"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33228..33230),aa:Gln,seq:ttg)
     gene            complement(33313..33393)
                     /locus_tag="VC394_000406"
     tRNA            complement(33313..33393)
                     /locus_tag="VC394_000406"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33357..33359),aa:Tyr,seq:gta)
     gene            complement(33454..33684)
                     /locus_tag="VC394_000407"
     CDS             complement(33454..33684)
                     /locus_tag="VC394_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEP5993902.1"
                     /translation="MSKKDKKIEIQLIDHKVMVNETKIDGYQLQIGKRVVGEIAELDE
                     KFAVLKNDGVDCFFKTLEQAVENIIENYNLNH"
     gene            complement(33748..34815)
                     /locus_tag="VC394_000408"
     CDS             complement(33748..34815)
                     /locus_tag="VC394_000408"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEP5993903.1"
                     /translation="MKKSYNRKKQKRGTMMTIAIDWDNLGFEYHKLPFRYISYYKNGQ
                     WDKGQLTEDATLHISESAPALHYGQQAFEGLKAYRTKDGSIQLFRPDRNAVRLQATAD
                     RLLMPQVSTEQFIDAAKQVVKANEDFVPPYGTGATLYLRPLLIGVGDIIGVKPAEEYI
                     FTIFAMPVGNYFKGGLAPTNFIVSEAFDRAAPYGTGAAKVGGNYAGSLLPGKAAKSAG
                     FSDVIYLDPATHTKIEEVGAANFFGITANNEFVTPLSPSILPSITKYSLLQLAEERLG
                     MTVIEGDVPINELDKFVEAGACGTAAVISPIGGIQYKDNLHVFYSETEVGPVTRRLYD
                     ELVGIQFGDIEAPEGWIVKVD"
     gene            complement(34893..37352)
                     /gene="parC"
                     /locus_tag="VC394_000409"
     CDS             complement(34893..37352)
                     /gene="parC"
                     /locus_tag="VC394_000409"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021340535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEP5993904.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQERALPDIRDGLKPVQRRI
                     LYSMNKDGNTFEKGYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKNTVSFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSSGISAGYATDIPPHNLSEVIDAVVYMIDHPKASLEKLMEFLPGPDFPTGGI
                     IQGADEIKKAYETGKGRVVVRSRTEIEELKGGKQQIIVTEIPYEVNKAVLVKKIDDVR
                     VNNKVPGIVEVRDESDRTGLRIAIELKKDADSQTILNYLLKYTDLQVNYNFNMVAIDH
                     FTPRQVGLQKILSSYISHRKDIIIERSKFDKAKAEKRLHIVEGLIRVLSILDEIIALI
                     RSSDNKTDAKENLKVSYDFSEEQAEAIVTLQLYRLTNTDIVTLQNEENDLRDLITTLS
                     AIIGDEATMYNVMKRELREVKKKFANPRLSELQAESQIIEIDTASLIAEEETFVSVTR
                     GGYLKRTSPRSFNASSLEEVGKRDDDELIFVKQAKTTEHLLLFTTLGNVIYRPIHELT
                     DLRWKDIGEHLSQTISNFATEEEILYADIVTSFDQGLYVAVTQNGFIKRFDRKELSPW
                     RTYKSKSTKYVKLKDDKDRVVTLSPVIMEDLLLVTKNGYALRFSSQEVPIQGLKSAGV
                     KGINLKNDDSLASAFAVTSNSFFVLTQRGSLKRMAVDDIPQTSRANRGLLVLRELKTK
                     PHRVFLAGGVQSDTSAEQFDLFTDIPEEETNQQMLEVISKTGQTYEIALETLSLSERT
                     SNGSFISDTISDQEVLVARTR"
     gene            complement(37443..39392)
                     /gene="parE"
                     /locus_tag="VC394_000410"
     CDS             complement(37443..39392)
                     /gene="parE"
                     /locus_tag="VC394_000410"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEP5993905.1"
                     /translation="MTKKEITINNYNDDAIQVLEGLDAVRKRPGMYIGSTDATGLHHL
                     IWEIVDNAVDEALSGFGDDIKVVINKDGSVSVADSGRGMPTGQHAMGIPTVQVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSAWLEVEITRDGSVYRQRFENGGKPVTTLK
                     KVGTAPKSKSGTVVTFMPDDKIFSTIDFKFNTISERLKESAFLLKNVKMSLTDLRGDD
                     PIIEEFHYENGVQDFVEYLNEDKETLTPVIYMEGQDQDFQVEVALQYNDGFSDNILSF
                     VNNVRTKDGGSHETGLKSAITKAMNDYARKTNLLKEKDKNLEGSDYREGLSAVLSILV
                     PEQHLQFEGQTKDKLGSPLARPIVESIVSEKLTFFLLENGEVASHLVRKAIKARDARE
                     AARKARDDSRNGKKNKKDKGLLSGKLTPAQSKNAKKNELYLVEGDSAGGSAKQGRDRK
                     FQAILPLRGKVLNTEKAKMADILKNEEINTMVYTIGAGVGADFNLEDINYDKIIIMTD
                     ADTDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKTEKVSYAWTDGEL
                     EDLRKEFGKGAILQRYKGLGEMNANQLWETTMDPETRTLIRVTIDDLARAERRVSVLM
                     GDKAAPRRQWIEDNVKFTLEENTVF"
     gene            39527..40168
                     /gene="plsY"
                     /locus_tag="VC394_000411"
     CDS             39527..40168
                     /gene="plsY"
                     /locus_tag="VC394_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEP5993906.1"
                     /translation="MKLLLFITIAYLLGSIPTGLWIGQYFYHINLREHGSGNTGTTNT
                     FRILGVKAGTATLAIDMFKGTLSILLPIIFGMTSISSIAIGFFAVLGHTFPIFANFKG
                     GKAVATSAGVLLGFAPLYLFFLASIFVLVLYLFSMISLASVVSAIVGVLSVLTFPAIH
                     FLLPNYDYFLTFIVILLAFIIIIRHKDNISRIKHHTENLIPWGLNLSKQVPKK"
     gene            complement(40226..41494)
                     /locus_tag="VC394_000412"
     CDS             complement(40226..41494)
                     /locus_tag="VC394_000412"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEP5993907.1"
                     /translation="MLLIKNGRVMDPKSQRDQVADVLIDGKQIVKIASAIECQEAQVI
                     DASGLIVAPGLVDIHVHFREPGQTHKEDIHTGALAAASGGVTTVVMMANTNPVISDVE
                     TLQEVLASAAKEKIHIYTNASVTQAFNGKDVTDFKALLEAGAVSFSDDGIPLESSKVL
                     KEAFDLANANQTFISLHEEDPQLNGVLGFNEGIAEEHFHFCGATGVAEYSMIARDVMI
                     AYDRQAHVHIQHLSKAESVQVVAFAQQLGAKVTAEVSPQHFSTTEDLLLIVGTSAKMN
                     PPLRTQRDRLAVIEGLKSGVITVIATDHAPHHKDEKAVDDMTKAPSGMTGLETSLSLG
                     LTHLVEPGHLTLMSLLEKMTLNPALLYGFDAGYLAENGPADLVIFADKQERLITENFA
                     SKASNSPFIGNKLKGVVKYTIADGEVVYPN"
     gene            complement(41618..42271)
                     /locus_tag="VC394_000413"
     CDS             complement(41618..42271)
                     /locus_tag="VC394_000413"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEP5993908.1"
                     /translation="MAHSIWHEKIKSFLPEHYYGRINHFLDEAYASGLVYPPRENVFK
                     ALQVTPLEETKVLILGQDPYHGPKQAQGLSFSVPEEISAPPSLINILKELADDIGPRD
                     HHDLSTWASQGVLLLNACLTVPAGQANGHAGLIWEPFTDAVIKVLNEKDSPVVFILWG
                     AYARKKKAFITNPKHHIIESPHPSPLSSYRGFFGSKPFSRTNAILEKEGMTGIDWLQ"
     gene            complement(42404..43054)
                     /locus_tag="VC394_000414"
     CDS             complement(42404..43054)
                     /locus_tag="VC394_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEP5993909.1"
                     /translation="MINIPLMKDSLGFVLSGLPYTLGISLLSFLTGLVFGLLLALLGR
                     SHQPLLHYLVRAYISIMRGVPMIVVLFVLYFGLPYYGLELPALLCAYLGFSMVSAAYI
                     SEVFRSSIEAIDKGQWEAAKALGLPYALMVKKIILPQAFRIAVPPLGNVIIDMVKSSS
                     LAAMITVPDIFQNAKIIGGREWDYMSMYILVAFIYWIIAFLLERYQEFLENKLALV"
     gene            complement(43083..43946)
                     /locus_tag="VC394_000415"
     CDS             complement(43083..43946)
                     /locus_tag="VC394_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEP5993910.1"
                     /translation="MKKSVTLLSIGLASLLLAACAPHKSQKSSWETIKEKGVLKVATP
                     GTYQPTSFYNDNNELVGYEVDMVKEIGKRLNIKVKFVETGFDQAFTSVDSGRVDISLN
                     NFDITPKRQKKYNISTPYKYGVGGMIVRADGSSNMAKKDLSDWKGKKAAGASGTEYMK
                     VAQKQGAELVTYDNVTGDVYLNDVANGRTDFIPNDYPAQKLFVDYMLSQNPNLNVKMS
                     DAQYNPTEQGIVMNKKDDSLKKKIDAVIKDMIKDGSLKKISETYYAGQDLTKPFGKDK
                     KIPIIDTKDVN"
     gene            complement(44064..45518)
                     /locus_tag="VC394_000416"
     CDS             complement(44064..45518)
                     /locus_tag="VC394_000416"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEP5993911.1"
                     /translation="MTYQDATAMAIAVQTGQTTPLELVTQAIYKAKKLNPTLNAITSE
                     RFEAALEEAKQRDFSGKLFAGVPIFLKDLGQELKGDLSTSGSKLFKNHHATKTDLFVK
                     RLEDLGFIILGRSNTPEFGFKNISDSQLHGPVSLPHDNTRNAGGSSGGAAALVSSGIS
                     ALATASDGGGSIRIPASFNGLIGLKPSRGRMPVGPGSYRSWQGASVHFALTKSVRDTR
                     NLLYYLQMEQMESPFPLATLTKNSIYQSLQRPLTIAFYQRLSDGSPVSLDTEKALRQA
                     VTWLREQGHQLVELEEFPVNMTEVIRHYYIMNSVETAAMFADIEDTFGRPMTKDDMET
                     MTWAIYQSGKDIPAWRYSQVLQKWDTYSATMASFHETYDLLLTFTTNTPAPKHGELVP
                     DSKLMANLAQAEIFSSEEQFNLVETMFGKSLAINPYTALPNLTGQPAISLPTYETKEG
                     LPMGIQLIAAKGREDLLLGIAEQFEAAGLLKIPQ"
     gene            complement(45715..46344)
                     /locus_tag="VC394_000417"
     CDS             complement(45715..46344)
                     /locus_tag="VC394_000417"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEP5993912.1"
                     /translation="MTLGSQIATQLLDIKAVYLKPEDPFTWASGIKSPIYTDNRVTLS
                     YPKTRDLIENGFVETIRAHFPEVEVIAGTATAGIPHGAIIADKMTLPFAYIRSKPKDH
                     GAGNQIEGRVLKGQKMVIIEDLISTGGSVLDAAAAASREGADVLGVVAIFTYELPKAS
                     QNFKEAGIKLITLSNYTELIAVAKLQGYITNDGLHLLKKFKEDQVNWQQ"
     gene            complement(46402..47094)
                     /gene="pyrF"
                     /locus_tag="VC394_000418"
     CDS             complement(46402..47094)
                     /gene="pyrF"
                     /locus_tag="VC394_000418"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEP5993913.1"
                     /translation="MKEERPIIALDFSSFEETKAFLDLFPAEEKLYVKIGMELYYAQG
                     PDIVRSIKSLGHNVFLDLKLHDIPNTVRAAMAVLKELDIDMATVHAAGGVEMLKAARE
                     GLGQGPTLIAVTQLTSTSEDQMRGDQNIQTSLLESVLHYSKGAAKAQLDGVVCSAQEV
                     EAIKAVTPTGFTCLTPGIRPKGSNIGDQKRVMTPNQARRIGSDYIVVGRPITQAKDPV
                     AAYQAIKAEWAG"
     gene            complement(47371..47907)
                     /locus_tag="VC394_000419"
     CDS             complement(47371..47907)
                     /locus_tag="VC394_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5993914.1"
                     /translation="MNVTEQNSHQILIQKLLVSIHYLTLFRDELKLVERTPSILGGEF
                     PAHLVQSELGDIVAAIDTLDMQQRLIESTFWYEESAFKLMNKTLDIVDNWIKGVDHLI
                     DLCQSKEVFQIIIGDKRIRVFGVLSDVFSSLKVSALSLKEAPIPDVLYEHIQRVNLEE
                     EAFIKYYQSPKVPTGEFE"
     gene            48129..49034
                     /locus_tag="VC394_000420"
     CDS             48129..49034
                     /locus_tag="VC394_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEP5993915.1"
                     /translation="MRIQQLHYIIKIVECGSMNEAAKQLFITQPSLSNAVKDLEMEMG
                     ITIFNRNPKGITLTKDGVEFLSYARQIIEQTSLLEDRYKNHNTGRELFSVSSQHYAFV
                     VNAFVSLLKRTDMTRYELFLRETRTWEIIDDVKNFRSEIGVLFINDYNRDVLTKLFDD
                     NHLTASPLFKAQPHIFVSKSNPLATKSLLNMDDLRDFPYLSYDQGIHNSFYFSEEMMS
                     QMPHSKSIVVSDRATLFNLMIGLDGYTVASGILNSNLNGDQIVAIPLDVPDEIDIVFI
                     KHEKANLSKMGERFIEYLLEEVTFD"
     gene            complement(49113..49901)
                     /locus_tag="VC394_000421"
     CDS             complement(49113..49901)
                     /locus_tag="VC394_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HEP5993916.1"
                     /translation="MTNNQTLDILLDVYAYNHAFRIAKALPNIPKTALYLLEMLKERR
                     ELNLAFLAEHAAENRIIEDQYHCSLWLNQSLEDEQIANYILDLEVKVKNGAIIDFVRS
                     VSPILYRLFLRLITSEIPNFKAYIFDTKNDQYDTWHFQAMLESDHEVFKAYLSQKQSR
                     NVTTKSLADMLTLTSLPQEIKDLVFLLRHFEKAVRNPLAHLIKPFDEEELHRTTHFSS
                     QAFLENIITLATFSGVIYRREPFYFDDMNAIIKTELGLWRQSIV"
     gene            complement(49894..50607)
                     /gene="deoD"
                     /locus_tag="VC394_000422"
     CDS             complement(49894..50607)
                     /gene="deoD"
                     /locus_tag="VC394_000422"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEP5993917.1"
                     /translation="MSIHISAKKGDIADKILLPGDPLRAKFIAENFLEDAVCFNEVRN
                     MFGYTGTYKGRRVSVMGTGMGMPSISIYARELIVDYGVKTLIRVGTAGAIDPEVHVRE
                     LVLAQAAATNSNIIRNDFPEFDFPQIADFGLLDKAYHIAREMGVTTHVGNVLSSDVFY
                     TNMPERNMALGKLGVKAIEMEAAALYYLAAQHHVKALGIMTISDNLNDPTEDTTAEER
                     QTTFTDMMKVALETLIAND"
     gene            complement(51192..52001)
                     /locus_tag="VC394_000423"
     CDS             complement(51192..52001)
                     /locus_tag="VC394_000423"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015647060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEP5993918.1"
                     /translation="MSLMTKINETKDFLVAKGIEAPEFGLILGSGLGELAEEVENPVV
                     IDYADIPNWGKSTVVGHAGKLVYGDLAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKA
                     LGCEGVLVTNAAGGIGYGPGTLMAITDHINMTGNNPLIGENLDEFGPRFPDMSDAYTK
                     VYRNKAHEVAEKMNIKLEDGVYMGLTGPTYETPAEIRAFKVLGADAVGMSTVPEVIVA
                     AHSGLKVLGISAITNFAAGFQSELNHEEVVEVTEHIKEDFKGLVKAILAEL"
     gene            complement(51985..52425)
                     /gene="arsC"
                     /locus_tag="VC394_000424"
     CDS             complement(51985..52425)
                     /gene="arsC"
                     /locus_tag="VC394_000424"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922177.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEP5993919.1"
                     /translation="MEEITIYHNPNCGTSRNVLAMIRHAGIEPTIIEYLQTPPNRETL
                     IELLQSMGISARELLRTNVPEFEAHGLANQAVAEKDVINAMLTDPILINRPIVVTRKG
                     VKLCRPSETLLDILPVPLPSPYIKEDGEIVNPIEVKGEKDVIDD"
     gene            complement(52441..53652)
                     /locus_tag="VC394_000425"
     CDS             complement(52441..53652)
                     /locus_tag="VC394_000425"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEP5993920.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDADKFFNAGVADTDSDTLGHISE
                     TAGLSVPNMAKIGLGNISRPIPLKTVPTEDNPTGYVTKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKIIREANKPYSGTAVIDDFGSRQMETGELIV
                     YTSADPVLQIAAHEDIIPVEELYKICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRHDYAVSPFQDTVLNKLADAGVPTYAVGKINDIFNGSGITNDMGHNKSNSHGIDTL
                     IKTLQLPEFTKGFSFTNLVDFDANFGHRRDPEGYRDCLHEFDNRLPEIIANMKEDDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSASFTGNGLIPQGHFADISATVAENFGVDIA
                     MIGESFLGHLK"
     gene            complement(53729..54412)
                     /gene="rpiA"
                     /locus_tag="VC394_000426"
     CDS             complement(53729..54412)
                     /gene="rpiA"
                     /locus_tag="VC394_000426"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEP5993921.1"
                     /translation="MEALKKIAGVTAAQYVTDGMTIGLGTGSTAYYFVEEIGRRVKQE
                     GLQVVGVTTSSVTSKQAEVLGIPLKSIDDIDSIDLTVDGADEVDKDFNGIKGGGAALL
                     MEKIVATPTKEYIWVVDASKMVEHLGAFKLPVEVVQYGADRLFRVFEKAGYKPSFRMK
                     GDSRLVTDMQNYIIDLDLGCIKDPVAFGHLLDGTVGVVEHGLFNGMVDKVIVASKDGV
                     TVLEVPKAS"
     gene            54790..56889
                     /locus_tag="VC394_000427"
     CDS             54790..56889
                     /locus_tag="VC394_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEP5993922.1"
                     /translation="MTHFSGKDPFVNMDDIFNQLMANMGGYRSENPRYLVNGREITPE
                     EFQHYRQTGQLPVATTKATTSQMLTPKADSVLTQLGTNLTQEARQGHLDPVIGRNKEI
                     QDTAEILARRTKNNPVLVGDAGVGKTAVIEGLAQAIVNGDVPAAIKNKEIVSIDISSL
                     EAGTQYRGSFEETIQNLIQEVKEAGNIILFFDEIHQIVGAGATSSDSGSKGLADILKP
                     ALSRGELTLIGATTQDEYRNTILKNAALARRFNEVKVNAPSAEDTFHILMGIRNLYEQ
                     HHHITLPDNVLKAAVDYSIQYIPQRSLPDKAIDLLDMTAAHLAAQHPVTDLKTLETEI
                     AKQKERQEKAVAKEDFEKALAAKTRIETLQKQIEQHNQSQNVTATVNDIAESVERLTG
                     IPVSNMGTNDLERLKGISSRLKSHVIGQDEAVAAVARAIRRNRAGFDDGNRPIGSFLF
                     VGPTGVGKTELAKQLALDLFGSKDAIIRLDMSEYNDRTAVSKLIGTTAGYVGYDDNNN
                     TLTERVRRNPYAIVLLDEIEKADPQIITLLLQVLDDGRLTDGQGNTINFKNTVIIATS
                     NAGFGQQDTETSGFNIMDRIAPYFRPEFLNRFNSIIKFNHLQKENLEEIVDLMLAEVN
                     QTTAKKGISLTIADDAKAHLIDLGYNHAMGARPLRRIIEQEIRDRITDYYLDHPEVKK
                     LQAILEEGQLVIRQKDQ"
     gene            complement(56947..57690)
                     /locus_tag="VC394_000428"
     CDS             complement(56947..57690)
                     /locus_tag="VC394_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5993923.1"
                     /translation="MTQVAQGRSKGKSLLCHQCNALFVFIIFLILLAVLFGVRYRNSS
                     IEGIWRTTSIDQKLGDDFAKRLTGLHQSPLIDDSLLTSSQMILTVKNNNVDLSFSVQV
                     ERDIFVKRLAAYHQNELLKTLKENHLVVGDLSSKERQIIENSMPASHELEMILDQAFE
                     KLASQIGGKYNQKTGHLSAVVLKGKVNRILHTIDIKEEVAAGHTSFSKGLLTPNGYFD
                     YTRFGKKLELLGDEKIIFKKALKKSPSSV"
     gene            complement(57838..58437)
                     /locus_tag="VC394_000429"
     CDS             complement(57838..58437)
                     /locus_tag="VC394_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEP5993924.1"
                     /translation="MAEEQVLNTHNASILLSAANKSHYPKDDLPEIALAGRSNVGKSS
                     FINTILGRKNLARTSSKPGKTQLLNFFNIDDKLRFVDVPGYGYAKVSKSERAKWGKMI
                     EEYLTSRDNLRAVVSLVDLRHAPSKEDIQMYDFLKYYDIPVIVVATKADKIPRGKWNK
                     HESVVKKALNFDNSDTFIVFSSVERIGIDDSWDAILEQV"
     gene            complement(58447..59676)
                     /gene="clpX"
                     /locus_tag="VC394_000430"
     CDS             complement(58447..59676)
                     /gene="clpX"
                     /locus_tag="VC394_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEP5993925.1"
                     /translation="MAGSRTNDIKVYCSFCGKSQDDVKKIIAGNNVFICNECVALSQE
                     IIKEELAEEVLADLTEVPKPKELLDVLNQYVVGQDRAKRALSVAVYNHYKRVSFTESR
                     DDDDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLIQAADYNVERAERGIIYVDEIDKIAKKGENVSITRDVSGEGVQQALLKIIEGTV
                     ASVPPQGGRKHPNQEMIQIDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNSRKID
                     DNASYMQEIISEDIQKFGLIPEFIGRLPVVAALEQLNTSDLIQILTEPRNALVKQYQA
                     LLSYDGVELAFDKEALEAIANKAIERKTGARGLRSIIEETMLDIMFEIPSQEDVTKVR
                     ITKAAVEGKSKPVLETA"
     gene            complement(59806..59976)
                     /locus_tag="VC394_000431"
     CDS             complement(59806..59976)
                     /locus_tag="VC394_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5993926.1"
                     /translation="MQRSIFGVFTAFLCVICVLCAIPAFKKNRYGLGSLFLLNAFTNL
                     VNSIHAFYGTLF"
     gene            complement(59996..60493)
                     /locus_tag="VC394_000432"
     CDS             complement(59996..60493)
                     /locus_tag="VC394_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEP5993927.1"
                     /translation="MTKEIIAIWAEDEAGLIGVAGKLPWYLPKELEHFKKTTLHQAVL
                     MGRVTFEGMNCKRLPQRQTLVMTRNRDYQVDEVLTMTSIEKVLEWYHAQDKTLYIIGG
                     NKVLEAFNGYFDRIIKTVIHHRFKGDTYRPNLDFSHFTQESQTFYARDAKNPYDFTVT
                     ALKHK"
     gene            complement(60573..61412)
                     /locus_tag="VC394_000433"
     CDS             complement(60573..61412)
                     /locus_tag="VC394_000433"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEP5993928.1"
                     /translation="MTKADQIFKANIQKIINEGSLSEQARPKYKDGRTAHSKYITGAF
                     AEYDLAKGEFPITTLRPIPIKSAIKELFWIYQDQSNSLDVLEAKYNVHYWNEWEVDQT
                     RTIGQRYGAVVKKHDIISKILKQLAENPWNRRNVISLWDYEAFEETKGLLPCAFQIMF
                     DVRRVGEDLYLDASLTQRSNDILVAHHINAMQYVALQMMIAKHFGWKIGKFFYFVNNL
                     HIYDNQFDQAQELLKRQPVASQPKLVLNVPDGTNFFDIKPDDFELQNYDPVKPQLHFD
                     LAI"
BASE COUNT        18586 a        13098 c        10823 g        19003 t
ORIGIN      
        1 tcacgtttta ccgaccttga cataaaaaaa cacatcattt taaaacctct ttcacacctt
       61 caaacaagta acaaagcgat aaaaataagc ttagcaaaac tgctaaagct tatttttatg
      121 attctatttg ttcagcgatg gtttcccatt cttccataag agtttcttgt tcttgatcca
      181 attggtccca ttccttttgc caagcgatta actgcgccgt atcattggag gcatgcatgt
      241 cttcttgaat agcaccaatg cgctcttcaa tggtttcaag tcttgcttca atctcctcat
      301 atcggcgggt caagcgacga cgctcttttt gattagcttt ttgtagctga taatcagtgg
      361 cagaggcttc ttttgtttcc tcaactgtct ctcctgctgc taggcgggct agttcttcta
      421 gctctgcctt tttctcaagg taataatcat agtcacctaa gtaaagggtt gagccatttt
      481 cggtaatttc cagaacctta gttgccaaac gattaatgaa atagcggtcg tggctgacaa
      541 aaagcaaggt accgtcaaag tcaatgaggg cattctctag cacttcttta ctgtcaatgt
      601 ctaggtggtt agtcggctcg tccagaacaa gaaaattatt gttttccata gataacttcg
      661 caagaagcag acgggctttt tctccacctg acagcatagc caccgatttt ttgacatcat
      721 ctccagaaaa gagaaaagcc cctaatcggt ttctgatatc gacttctggc gtagttgaaa
      781 aatcctgcca taattcttcc aaaactgtgt tgctagacgt caaatgtgat tgggtttggt
      841 catagtaacc tatttcaaca ttggcaccgt atttcacttc tcctttgatg aaaggaatct
      901 gtccaataac agatttaatc agagtagatt taccaatacc atttggacca acaatagcaa
      961 tcgcatctaa tttgtgaacg tctaggttaa taggctcaga taaaacatgt tggttataac
     1021 caatagcagc tttttcaacc gtcaacacca cattgccaga cggcttatct gcctgaaaag
     1081 tcatgtgagc tgattttcta gcactagtcg gtttgtctaa gcgttctatt ttttccaatt
     1141 gcttacgtct cgcttgagca cgttttgtag ttgaagccct gacaatattt ttttgaacaa
     1201 aatcttctaa cttggcaatt tctttttgct gtttgtcaaa ctgcttttct tcggctacta
     1261 atttttcagc ttttaaggtc ataaaacgag aataattgcc aacgtagcga tccaatccat
     1321 gaggagttaa atccaaggtc actgttgcca ccttatccaa aaaataacgg tcgtgactca
     1381 caatgattag agctccttga taattggcca agtagttctc taaccaggca atggtttcaa
     1441 tatctaagtg gtttgttggc tcatcaagga ccaatagttc tggcttttct aaaagcattt
     1501 tagccaaggc cagacgagtg ttttgacccc ctgacagctc agcgatagtc atctgccaca
     1561 tgctttcatc aaatttaaag ccatttaaaa tggctttaat ctcagcttca tatgtaaagc
     1621 caccttgctg acgaaaacgt tctgctaaaa gatcataatc catcatcagc cgtgtcaagt
     1681 cctgacctga aacggttgcc atatccattt ccatctgacg gagacgcttt tcatcttggc
     1741 gtaatgcttc gaaaaccttg agcatttcct catatatcgt tttgtcagat tcaaaacggc
     1801 tattttgagc cagataagac aaggttaggt cttttttagt attaacttct ccgctcgttg
     1861 gggtttcttc cccaaccaat agcttgagta aggttgactt accagctcca tttggcccca
     1921 ctaatgcaat tctgtctctt tcatcaactt gcagtgatat gttttgaaaa agaacatctc
     1981 ctgagaaaga gcgttctaac ttgtttcctt gtaaaataat catactttca ttctaacaaa
     2041 aaaatgccct tttgaggaca ttttcagcct agtttccaaa agaaatcgaa ctctttttaa
     2101 gtcgttgatt accttcttta gcgctaacaa ggaaagtgga atgactatta ccactcccat
     2161 gacgcgtctt tgcagatggc ttttgtgtta agatatcaaa aatttgcacc acttgctcag
     2221 taactatcga gatcttattg gctaaaacgg aatcactgtg attagaatag tacatcagcc
     2281 ctataataat aatcgttact aataagatag tttgtaaaaa ccctttgata agatgccaaa
     2341 acattttcac tattagctta ggcagccaca atatgatcgc cagtaattgt ttcatttgtc
     2401 acctcatgtt gtactaggaa atggttaaga caacctttct tctttttggt agtttatcgg
     2461 taaccactct aacaggactt ctaattgctg gttccaatag taccactcat gtttaccatg
     2521 acctttatga taatcaatgt caagaccttg agcctgaaaa tcagcaatag ctttttcatt
     2581 agtggcaaaa agaaaatcct catagccaca ccaagcataa aatttggttt ttccatcact
     2641 ttctgccacc atatttttaa ggtaatgctt gtcaaggtca gggtcatcaa actggccaaa
     2701 aacgccctgc caataggcta attctcccag gtttccctca agcaaggtct caggagaaaa
     2761 atccaaagcg cctgaaaacg atgccgcata agaaaaccga ttgcttttga gtgcccactt
     2821 aaaggcacca tagccaccca tagagagccc agcaacaaag gttttttctc gtttctgggt
     2881 catattagga aagaaagctg ctaagacctg cggtaactct tgtgacaacg cccgataata
     2941 gttaagtcca taagccgtgt ctgtatacca acctaggtct gtcgaaggca taacgactat
     3001 caaattggta tgccgcaaca agcgttcaat agctgttcgt ttttgccaag agttttcatt
     3061 gcctcccata ccatgtaaca aatataaaac aggaatatcc ttatcccctt gatctttttt
     3121 agggatttca gactggtcag gataaatcac atttacctta cgctccattc ctaaaactac
     3181 cgaatggtat tcaattgcaa tacttgccat tcattacact cctttatacc aaaataatcc
     3241 aatcacgtaa atctactgta tttttgggtg aacagtttcc tggtttacca gttgttaaaa
     3301 tcaaagaagg taggcagttt ttgcctaacc ttcctatttt cactaacgaa cttccatcac
     3361 aaccggcaga atcgctgggc gccttttggt ttgttcaaat aagaatttag acaaatcatc
     3421 tctgacattc ccttttaatt cgccccagtc aaaggtatct ttttttaagt aattgccaac
     3481 agttgtatta actaattctg cactttctct taaaatatca tgacttttct tgacatacac
     3541 aaatccacgc gtattaactt tagccttaga aataatgcgt ttttcttttt tagaaacagt
     3601 aatagcaaca atgaaaatac catcttctga caagacttta cggtctcgta aaacgatatt
     3661 acccacatca ccgatagcat taccgtcaat catgacatca ctggcaggta cacctccttc
     3721 atggagaaaa ccttcgtcat taagcaccat gatatcgcca cgcttcagaa tatgaatatt
     3781 ctctggaaaa ataccaactt cttcagctag ctttgcatgg gcagctaaat cacgatattc
     3841 tccttgcact ggaaaaagat actggggttt taacaaattc atcaatagtt gcaaatcacg
     3901 cccattagca tggcctgaca cgcgtaagtt ttgagtgatt aatttaacag aaccaccggc
     3961 cttgtaaatc aagttttcta cacgcgccac catagcttct ttagccgtac ttggtgtagt
     4021 tacaatataa acaaggtctc cttctttgat ttgaacataa cggtgacgtc ccgctgccat
     4081 tttttgtaag ctattaattg gctcacccat acgaccagct tcaaggatga ttaattcatg
     4141 gtcttcgaat ttagacatat ccttaggttt aattaaaaga cgctcatccg taatcattaa
     4201 tttttctaga cgtaaagctg ttctgacaat attttctgca tctgttccgg ttaaaacaac
     4261 acgacgccca tgggcagtgg cggaatcaaa tacttgctga atccgaacaa gatttgaggc
     4321 tactgcagct acaatgacac ggccatcagc atcagagata acactgtcca tttcttcacc
     4381 aacttcagat tcgctggcaa tctgatcgtt gcttgtcgca ttgactgaat ctgataaaag
     4441 agctagaacc ccttctttcc caatctcagc taaacgtagc aaatccgtct gataaccttc
     4501 tcttgctgct tgatcaaatt tgaagtcacc ggtataaaca atgtttcctt tatctgttcc
     4561 aataacaata cccatacttt ctgggattga atgcgttgtt ctaaagaacg aaactaggcc
     4621 atctttaaat tcaatttcag tatcactgtc taccacatga aaattattaa atttcttagt
     4681 gctgttatta cttttcacaa atagcttggc taattcaata gtcaattcag atccaaatac
     4741 aggagctgat acttcagcta acaaataagg aagggctcca atggcgtcag catggccatg
     4801 actcaagaaa atcccttgta ctttaccttt gttttcaatt acataatcta aattggggat
     4861 gactaaatct accccaagtt gctcattttc agggtacttg agccctgcgt ctaaaataaa
     4921 catagaatcg ttaatttcta ccaaatagaa gttttttccg tattcacgga cccctcccaa
     4981 ggcaatcatt ttaatatcag tcatgatact cctttaaata ttttattatt acaaacggtc
     5041 cttacaacgt aagtcgaatt actgttatgt agctatgcta ctatcttcta tagtatatca
     5101 tatttgaggc gttttttctc taaatagcct tgatactttc agattatttt cagtatggaa
     5161 atcaaaaaat cactgactta ttattagtca atgatttaga tcgttgatta gtcaaatagt
     5221 tggtaatagt cagtaatcgt cagttgttct ttttccattt gatttaaatc tttaataatg
     5281 tttccatctt tcattacaat cagacgattc ccataagtta atgcatcttc catatggtgg
     5341 gtaatcatca aagctgttaa gccatctttt gtcacaaact catctgtcaa ttgcattaaa
     5401 gattgactcg tcttagggtc aagggcagcc gtatgttcat ctaataataa gagagctggc
     5461 tttttcaacg ttgccattaa taaacttaaa gcctgtcttt gtccaccaga caaaagacct
     5521 gctggtgttt ctaaatgctt ttctaggcca tttcctgttc gtttaaccaa atcctcaaaa
     5581 ctagctaagt gctctgttat ttttcgagaa gccaaggagc gtttttctcc tctttgacgt
     5641 gcgattaaga gattttcagc cactgtcatc cgaggggctg ttcccatttt gggatcttga
     5701 aagactcgcg ataggtaaag agctcttttt tctgccggcc aatgcgtcac atcctgacca
     5761 agaatcctaa tttgcccccg tgtcaaactt agcgttcctg caattacatt aaaaagggtt
     5821 gattttccag ctccatttcc tcctaaaata gttaaaaaat catgttcata gatagttagt
     5881 gtcacattgt ccagtattgt tttagcatct tcaaaaccat tatctacgtc aactgttgca
     5941 ttgatcaatt caataatttt tgtcatcgtg ttaacctcac tcccttaaag tagcgctctt
     6001 ttaccactgg tatcatgagg cacagtgcta aaacaagagc actaattaat ttcaaataac
     6061 ttgtattaaa acctagcgta ataactactg aaattaaaaa ttgataaaga atcgagccta
     6121 ttacaatagc aatcaaacgt tctaaaagag tcagtccagt tgagtatagt acttcaccaa
     6181 caattatgct cgccaaacca ataacaataa ctccaatccc ttttgagaca tctgcatagc
     6241 catcttgttg gctcactaga gcacctgata aagctattaa actattagaa accacaagtc
     6301 ccataacttc catccaatcc gtgttaatac caaaactctt agccatatct ttattgtctc
     6361 ccgtagcgat ataagcttga cctagattag tgtaaaggaa atagatcaaa acgctaataa
     6421 caatcacaac agttatcaga cctgttaaaa ggctatttaa atcaattgaa aaaggtaaac
     6481 aatcctgaat acgtttatga tcatgtaaac ccaagtttgc tcttcccatt accattagca
     6541 taatggagtt acatgacgtc atcaccaaaa ttccagctaa aagtgttggc atttttcctt
     6601 tcgtatataa cagccctgtt aaaaaacctg ctaaagcacc tgataacata ccaagtaagg
     6661 ttgatagaaa tggattccac cctaagctaa tagcggtgac cgcaacagct cctcctaaag
     6721 gaaaagaacc ttctgtagtc atgtcaggga agtttaaaat cctaaaggtc aggtagattc
     6781 ctaatcctaa aactccccat ataaggcctt gtgaaactga tgaaataatc atatttttcc
     6841 tcatatttat attagcgggt aactctcgcc agtataaacc ctttctttac attaattttt
     6901 agatttgttt tatacttagt tcagcaccgt ggcttagtct tttttccaat cactttaacg
     6961 aaactaagac agaaaacaaa aaaatcgaga atcatgttta aacttactca aaaggcaaga
     7021 ttttttacgt gtctgaaata gagcatttta aaactaaaaa tgataaaaat aacccttaca
     7081 tcctcaagac gattgtctga cttggcatcc aaattcatct cagtgagggt gttttttatg
     7141 gttttttctt gaccttttat atcacatgtc attgtattaa ttctcaataa aagaagctga
     7201 aggaaaatct tccctgttaa tagcaatacc taattctgct gctacattct tattaattag
     7261 cggttgacca atatccacaa ctttgacagg aacatctaca acacgctttc ctttgattaa
     7321 ttttaacacc tgattagctg tttgaactcc caaatcgtac tgattttgag aaatggctgc
     7381 caaaccacct ttttcaacca ttcgatcaac gctagtgtag acaggaatct ttgcagcctt
     7441 actggttgtc atgactgatg aaaatgcact tgctatagta ttgtcttggg ggataaatac
     7501 cgcgtctact ttacctaaca taactgacat cgtggcagga acttcgttag tagaaggaac
     7561 cgcgtatgaa ataacttgat agcccttttt gcgagctaac cgcctaaact ctttaacttg
     7621 agataaggaa ttatcctcat tacttgcata taaaattcca actgttttga catgtggtgt
     7681 cagatttttc attaaatcaa ttgtctgttt aacaggcact ttattagata gaccagttac
     7741 attagcttca ggttgatcca attgcatcac taatctagat cctactggat cagatactgc
     7801 tgacatgaca actgggatat ccttgctggc agctgctaag ccttgcgccg ccggggttgc
     7861 gataccaatc acgatatcac ttcctgattg cactaattgc ctactcattg tttggatttt
     7921 actctgatcc ccctcagcat tcataacctt aatcactaca ttttgttttt gaggcatctg
     7981 ctttttgagt tgatcttcaa tacctcgctt aatatcatca agagcctcat gagtaacgaa
     8041 ttgtaagata ccaattgtaa tgttttgtcg gttagccaaa tcacggtttt cctttgatac
     8101 tccttttgat aatagagagc ctatcactat tacagttaaa acaaggaggg tagctattaa
     8161 actcttgttt ttcatctgaa ttcctcacat ttcatgtcaa tctctttaaa acgttgatga
     8221 tgagaatact tctcaaaaga aaaagcactt agtaatgatt actaggcgcc cttgtgaaat
     8281 ccaatgtcag cgcatagata tctttttaga agtactctaa aagtcgtatc tatgccaatt
     8341 cataaacaca acttatctat gccagccttg ccaatttact aagtgttgtg tttttaccat
     8401 cattgacttc ctttctcgtt tttttaacat tataacctaa atcaaaaaaa ttgcaagctc
     8461 tttcgtccat atttttagtc aagtatttgt ggtataatga agagattaga attcagaaag
     8521 gagaaaccat gtcttttgac ggctttttct tacaccattt aaccaacgaa ttaaaagaga
     8581 acttgcttta cggtcgtatc caaaaggtca atcaaccctt tgaacgggaa cttgtcttaa
     8641 ccattcgtaa ccatcgcaag aattataaat tgctattgtc tgcccatcct gtttttggaa
     8701 gggtacaaat cacccaagca gactttcaaa atcctcaggt acctaatacc tttaccatga
     8761 ttatgcgtaa atacctccag ggtgcagtca ttgagcagtt agaacagatt gataatgacc
     8821 gcattatcga aatcaaggta tctaataaaa atgaaatcgg agatgctatt caggcaaccc
     8881 taatcattga aatcatgggg aagcacagca atatcatctt ggtggaccgt gcagaaaata
     8941 aaatcatcga atccattaaa catgttggct tttcacaaaa ctcttaccgc accattttac
     9001 ctggctctac ctacattgag ccccctaaaa cagctgctgt taatcctttt acaattacag
     9061 atgttcctct atttgaaatc ctccaaacac aagaactaac agttaaaagc ttgcaacagc
     9121 attttcaagg tttggggcga gacaccgcaa aagagttggc cgagcttctg accacagaca
     9181 aattaaaacg gttccgtgag ttttttgcta gacctactca ggcaaatctg accacagctt
     9241 cttttgcgcc tgttcttttc tcagatagcc atgcgacgtt tgagacttta tcagagatgc
     9301 ttgatcactt ttatcaagat aaggctgagc gtgaccgtat caatcagcaa gccagtgatt
     9361 tgattcaccg tgtgcaaact gaactggaca aaaatcgtaa caagctaagt aagcaagaag
     9421 ctgagctgct agccactgaa aatgctgaat tattccgtca aaaaggagaa ttattgacca
     9481 cctacctcag tctggtccca aacaaccaag actctgtgat tttagacaat tattatacag
     9541 gggaaaaaat tgagattgcc ttagacaagg ctctgacacc aaatcaaaat gctcaacgtt
     9601 attttaaaaa ataccaaaag ctaaaagaag ctgtcaaaca cttatctgga ttgattgccg
     9661 ataccaagca aagtattact tactttgaga gcgtggacta caatttatct caagcaagta
     9721 tcgacgacat cgaagatatt cgcgaagaat tatatcaggc tggctttttg aaaagtcgcc
     9781 aaagagacaa acgccataaa cgtaaaaagc ctgagcagta cctggcttca gatggaaaaa
     9841 ccattctcat ggtgggacgc aataacttgc aaaatgaaga actcaccttt aaaatggcta
     9901 aaaaaggaga actctggttc catgctaagg atatccctgg aagtcatgtc atcatcaaag
     9961 acaatcttga cccaagtgac gaggttaaaa ctgatgctgc cgaactagct gcttattatt
    10021 cgaaagctag gttatcaaat ctcgttcagg tcgatatgat tgaagctaaa aaattacaca
    10081 aacctagtgg cgccaagcca ggatttgtga cttatactgg tcaaaaaact ttacgggtta
    10141 ctcctgacca agccaaaatt ctctctatga aattatcctg ataagcaact aaaaaacagc
    10201 tctcctagga aagttatttc ccctaggaga actgtttttt tattctgatt gttggtgctt
    10261 gatatggtca agaaagtctt gcatattcac atcatcaggc acttgtctga ggtaagcggt
    10321 agccatttgg atagccttgt gaaactggcc aaactccctt aataaatagg cataatcctg
    10381 taaaaactcc ggattttctg ccaaatcagc cgaaatctca ttataaagag ccaaggcaac
    10441 ttcttcttgt tccaaggcgt ggtaagcctt tgcaatggtc catttggtta agacattatc
    10501 aatcgtctca cgattaagag caataacctc ctcaaaacgc tcggcatcaa aataaagggt
    10561 cactaaacgc atgagaatct cttcgtcatc aacagctact tcctttgctt gaagcaggta
    10621 attttcagca ttctgcctat cgtgtaattc ataagaaagc tgcgatgcta gtaataataa
    10681 ttgactgtca aatgcatttt tacgtaggcc ctgttgaacc aagcgcaaag cttctgacgt
    10741 cttatgctct tcatgaagac tcaaagcata accatattca taacctggat aatctggatt
    10801 gatggtttcc aactgtttaa agtaaagatt agctttttga taattttctt ggtcatacaa
    10861 gagggtcgcc aattcaaaaa cagtttcatc ttcgtattca atggccacgg ccttttccaa
    10921 gaattcgatg gctgcctcaa atttccctaa gctagcatag gctcgtccaa tgcgttgata
    10981 agtggaaata cctgtcaatt caaggatttg acgattgtct aactgtgcat agtaatcaat
    11041 agcttcttta aaatactgca aggacatgtc aatttctgcc aaaccaaaaa tgaccaatgg
    11101 ctctgggctg atgctcactg cttgaagcaa tttttcacgc gccacctctg tcagtccttc
    11161 catgtcatag aggtcagcca ttactaagag agctgacaaa taattggggc tatcttttga
    11221 aaccttatcc aaatacaaga aagcttcttc aatggcgtcg tcttctgctg caatctgagc
    11281 taaatttata ttgagttctg ggtaatcatc agctaactgc aaataaatac gcttggcatg
    11341 tggcaaaaaa cctatacttt ctaagtattc tcctaaggcg attaaactat ctgcatcatc
    11401 ttcttttaaa gctttttgga aatatttttc agcgtgcgct aggtcttgct gatccaagga
    11461 agcaatcatt ttttcactat ttaacatggt ccttaacctc ctcaatcacc aatacttctt
    11521 cgtcatacaa accgctaact tttttgtacc agtcaaataa ttctttaatc acaactttga
    11581 cagaagcgta gattgggatg actaagaaga caccccaaac gccaaacatt gatcctgcag
    11641 tcaacaataa gaacatgatc gtaatcggat gaatgctcag cttatttcca agaacaagcg
    11701 gtgccacaaa acggccttcg atggtttgct caatcataaa gataacaagc accttaacca
    11761 acataaatgg gccttgtacc atagccataa tcacaacagg tatcatggca aggaaacttc
    11821 ctaagtaagg tatcatgtta agaaagccag caataatccc aaaagtgatg gcatatttca
    11881 gtcccacaag actaaacatg atagaaaaca taaagccgac aacaattgca accgtaacct
    11941 gcccttggac atagcctgat aattgcttgt taatatctcc caagactcga accataggca
    12001 cccgtaattt gagaggtaag acattcacaa ggccattttt catgtggcta ctgtctcgta
    12061 aaaaataaaa gagaataaag ggcgaaataa taatagccac ggtaatccga gcaatggcac
    12121 cagcaaaatt accagcccag tcaatagcat tttttgaaaa actttgcgcg taatccaaag
    12181 ccttttgact tgtatgggta agcatgtctt ggagttgggg cttgtagctg actaaccatt
    12241 cattttcaag aagtttattg gcttcttcat tgaccttgcc cacatatttg ggcagatcct
    12301 caataaaact ggtaagttgg gtctgtacca tgggaacaaa gcctgaaatc gcccagacaa
    12361 aaagtaaggt aatcaaacca aaaacaataa aaatagaggt ggtacgattc gggcccaagt
    12421 gattaataag gtcaactaag ggtttggtca gataatacag aatggtcgaa ataaccaagg
    12481 gcaacatgat gaccgcaaaa aaagaaatca ctggcataaa taaaaaagag attttagtaa
    12541 aaacaaaaat cgttaaaaag gctaaaagtg ttattactaa agccatggta gcttgattat
    12601 ttaaaaacca tttgtaaaat aagctatctg ttctagactt gtcaggtatt aagggcatta
    12661 agatagggtt cctttctttg ggaaaatgat aagttttact ggtaacctgg gtaaatcaat
    12721 aaaaggtcac agatttatcc atgagttggt tgttttggtc ataggtaaat ttggcagaga
    12781 aaaaaggacg gtcttctaaa atccatttaa aaatgtcata atcaccttcc caagttggct
    12841 tttccaacac ttgatcgtaa ggtacccatt ccaaggttcc ttctcgtgac tcttcatcgg
    12901 aaatgaggtc tccttcaaat cctgttactt taaaaacata ggtataccag tcatgaccag
    12961 gggtaaattc tggaaaagta atgattcctt taaaggccat ctctgtaaca gtcaaatggg
    13021 tttcctctaa aatctcacga cgggcacact catctggggt ctcaccagcc tcgagcttac
    13081 ccccaactga aatccacttg cctttgtgga catcattttc ttttttattg cggtgtaata
    13141 atagcaaaga atcgccatta tcaatgtaac aaatggttgc taattgagtc atctatctct
    13201 ttcctttcca tctttgtttc tattatacca atctctagaa aatatgctat cattttatga
    13261 gtcaaagtgt gaatacttag tcacgctcaa aaccttctaa agatgcctta aaaaagtgta
    13321 gataagagcc tacaatgaaa aacatcaatt taaactatca aaagaacctt gtcatatcaa
    13381 cgataattga tatgacaagg ttctttaatg ttttatttaa tcacttcttg agttttagca
    13441 tacttggctt ctacagcatc tttttctgct ttccaccatg tctcattttc tgtataccac
    13501 ttaatagttt cttccaaacc ttctgaaaag tttgtaaatt gtggttccca gccgagttct
    13561 tcacgcaatt ttgtagaatc aatagcgtaa cgaagatcgt gaccagcacg gtcagttacg
    13621 tgatcataag cgtcttttgg ttgacccatt ttctcaagga taagctcaag aacttccttg
    13681 ttgtttttct cgccgtcggc accaataagg tatgtttcac cgatacgacc cttagtcaaa
    13741 atagcccata ctcctgtaga atgatcatta gtatgaatcc agtcacggac atttttccct
    13801 tcaccgtata gttttggctt gatgcctgat aaaatattgg tgatttggcg aggaataaat
    13861 ttctcaatgt gctggtaagg tccgtagtta ttggaacaat ttgaaatggt cgctttcaca
    13921 ccgaaggaac gtacccatgc cttaacgata aggtcagaag ctgccttagt tgatgagtaa
    13981 ggtgatgatg gattgtattt tgtttcagca gtgaattttt cacctggtcc ttcaccctgt
    14041 cctggaaggt cttcacgaag tggaagatct ccataaactt catcagttga cacgtggtgg
    14101 aaacggatat cgtatttacg agctgcttca agcaaagtgt aagttccgat aaagtttgta
    14161 tggataaatg gacttggatc ttccaatgag ttatcgttgt gactctcagc cgcatagtga
    14221 acaatagcat ccgttttggc agccaatttg tctaccaatt cagcgtcagc gatatcacca
    14281 acaactaact caacacgatc accaagaata gcttcaatgt tagcacggtt acctgcatat
    14341 gtcaatttat caaggacagt tacatgaaca tctgggtggt tattgtagac atagtgcaca
    14401 aagttagatc cgatgaaacc agctccacca gttacgataa tatttttata cataagatat
    14461 aaagggacct ttcgtttccg aaaaaatcta ttaaaagtaa taactctaac aaagacgttt
    14521 taggcctaag aaaacatcgc tttctaagat ttgagtccca tgttcagctc aaagagagaa
    14581 actgaacttt cctttcatca tttttagttg ttacttgtta cctgaagtag agaagaataa
    14641 ctcccctcat atccacctca aatgattaat cccaatcatc tgagtatgtt gtttccctat
    14701 aggtcttttg gtttcagtgg tttgacatca ctaagaagag gatggttttt atctgcttca
    14761 gaaacttctg cagctgctag attttcccaa gtaatcccaa gacttgggtc agcatagttt
    14821 acaaaagcat acttaggttt caagtcaaga gcccagtagt cattgacaag ataactgtaa
    14881 gagacagtct ctgaaagaac ttgaaagcca ttagcaaccc ctctaggaac aaacattcct
    14941 tttgaagcat caatcactgt ctggtagacg tttccaaaag tctctccctc acgaagatca
    15001 acccaagctc ctaaaacctt gccatcatca gcaactgaga tgtatttatc ccaaggttca
    15061 gcatgaagtc cacgcagcac atgctgacgt gaaaaagaaa cgttgttttg tagttttcct
    15121 tcctcaaaaa aacgttctgg aaagccaatt ggcaacattt tttctttttg gaaattttct
    15181 ttaaaccagc cacgattatc cccacgaaca ggaatatcaa actctagtaa gcctgggatt
    15241 tctttgattt ctcggcaagc caatggtttg tcaaaaaaag tttctgtcat taagcttctc
    15301 caatcaaacg aagcaagtat tgcccgtatt cgtttttctt taaagactgc gccaattcat
    15361 ggacatcttc tttactgatg taacccatgc gataggcaat ttcttccaga tttgccactt
    15421 gagcgttttg caaacgttga actgtttcaa tatattgagc cgcttcaagt agactttcat
    15481 gcgttccagt gtctagccat gcaaaaccac gccccatcaa ttcaacggag aggtcaccac
    15541 gttccaagta agccttgttg acgtctgtaa tttccaactc accacgcgcg cttggtttaa
    15601 tgtttttagc aatttctacc acgtcattgt catagaaata aagtccagtc actgcaaagt
    15661 gggacttagg cacttctggt ttttcttcga tagagatagc attcatgttc tcatcaaatt
    15721 ccaccactcc aaagcgctca gggtctttca cttgataacc aaaaacggtg gctccttttt
    15781 ctttggcagc tgccttttga agcattttag tgaggccatt accgtggtaa atattatctc
    15841 ctaaaataag agcaacacgg tcatcaccga taaactcctc tccaataatg aaagcttgtg
    15901 ctaaaccatc tgggcttggt tgttccttat aggataagct aatgccaaat tctgacccat
    15961 ctcctagcaa ttcttcgaaa cgaggaagat cttggggggt tgaaatgatt aaaatatctt
    16021 taatccctgc caacatcaag gttgacaatg ggtaataaat cattggtttg tcataaatgg
    16081 gcatcaactg tttagatgcg gcacgggtca gtggatacaa gcgtgtccct gaacctcctg
    16141 caagaataat acctttcata cgtagtgctc ctattcttct atatacccta tcattctatc
    16201 attttttgaa agcagtgtca cgctttacca gtagaccgca aaaggtaagg ctcaggggaa
    16261 tagtcgctag gcgttaaaag tccttcgtgt cctagcaata attgagctaa ttcatagcca
    16321 atcaaagggc caactgttaa tcccgatgac cctaaaccac tagctgtata aaggttcttc
    16381 aatcctggaa cctgaccata aaaaggagaa taatcactgg tataagctct tatccctaca
    16441 cgacatgatt tttgagtagc ttcttttaat aacggcaaat aagtgagagc ttgcatttca
    16501 agtttttcta agacctgcca atcaggctcc aaatcataac ctttatcatt ttcatggctc
    16561 gcaccaacag aaatcttacc ttgattgaaa ggaatgaggt ctatttcacc ttctggcata
    16621 acaacgggat aattgtcgct gatgatgtga tgaacgtcat aatccaagag ttgtcccttt
    16681 tgtggtcgca catcaacctg atagcctaaa ggcatcaata agtcaggtag ccatgctcct
    16741 gctgccagaa tgacctgatc aaaataatga ccagcaatct cataaccact tccttgctgt
    16801 ttcagagtga ctttttggcg aataactgga taaccactag tcttgagtaa cgtctcacat
    16861 aaagcggctc cctccactct agcagctcct gaagcgtaca gacagttatc aaagccaatc
    16921 aatcctttaa aatcatcccc cagttcttta cgatttttaa tggctaactc accaataatc
    16981 ggtgattcta cctttcttgc taacgctaat tcataaagat ctcttagttt ttcttcctgc
    17041 ttttttaaaa catagatgcc attttgctga taaaaatctg ttgcaaaacc atcttctttg
    17101 aggtcattga ttaattgctg ataaaagtcc gctcctaaac gagccatgcg gtaccaaact
    17161 ttattgcgac gctttgaaaa ccaaggacta ataataccag cagcagcctt agtggcttgt
    17221 ccttgaccat ggtcaaaaat ggtaacttct ttttgaccac tttgttgtaa ataataagcg
    17281 gcggttgatc ctacaatccc cgccccaata atggctattt tcattcctgg tatcctttta
    17341 tcttacttta agaccttaac agcctataaa tggctaaatg gattggtcga ggctttgctt
    17401 gaaataatgc tgacatccca accgttctct tctttccacc cttggagttt ctcctttaat
    17461 ttctccgtaa aaagcacctc aatatgatgc cccgggtcaa ccgcaaatag tccttctgtc
    17521 agcatctcct gtgctgtatg gtaatagata tcccctgtaa tataaacgtc tgcgcctttt
    17581 tgcacagcat cctgataaaa ttcaccacca cttccgccac agatagcaat ctttgaaatc
    17641 aaagggtttt ccttatcgta acggataagt cgcacggtat ctaaatcaaa cacccttttg
    17701 accttacttg ctaactcttc taacgcttgt tctttaactg ttccaatacg accaatgcca
    17761 aagccttctt tggtttcaga caagtaagtt gcttccttga tttctagcag gtcacaaaac
    17821 caatcattta gcccgcctgg tacaatgtca atattggtat ggctcacata gacagaaatg
    17881 tcatgcttga ccaaatctag aaggatatca cgctgagggg atgaaaccag atctttaagc
    17941 ggtttaaaga tgggagcatg cttagttata atgagatcaa cttcattttt gatggcttct
    18001 gctactgttg actctcggat atctaaagta atcataacct tacgaatgtc tttatccaat
    18061 gaacccattt gaagaccttt aacgtctctt tccatagata aatcaagtgg gcaaaaggct
    18121 tcataggcat caattagggt tttagctttc atgaacaatc acctctttta tttgttgtat
    18181 ttttgtcaag agaagctgac gctcttgagt tttttcttct gggatgcagg acaaggcata
    18241 agctaactta tccatttctc gttgccactt ctctttaaaa acgactgatt tttcctgaga
    18301 aaggtatgga ccaaaacgca attctgtcgc ggataaggcc ttttcaccat gctcagcaac
    18361 gatgatttca taatatttgt cattttcagc catgatggtt tctgcaacaa ttttaaaagc
    18421 gttaaccgat aaccaagccc gtaaatcatc ctcacgattg ttgggctgta aaaccagacg
    18481 ttcaatgccc tgcaattttt ccttacctgc ctctaaaata tcagtaatca aacggccacc
    18541 catgccacaa atggtaatgg ctgtcacatc atcagcttct tcaaaggctg caaggccatt
    18601 ggctaagcgc acctgaatat gctcggcaag gccagactgt gtgacatttt taagagcaga
    18661 ttcgtaggga cccctaacca cttcgccagc aatggctgct gaaatctgat tagtttctac
    18721 caaaaatatg ggaagatagg catgatcact gccaacatct aataatttga caccttttgg
    18781 aacataggcc gcaacttggg ctaaacggtt tgataattga ctatccatct tgatttcttt
    18841 ctaaaacgta atggttctat tatagcaaaa ttacctccat aaaaaaagaa aaccaaaggc
    18901 taggctttcg attttccata agtattttga tagtacttac agtaagactg aacgggacaa
    18961 atctcacatt ttggtttttt ggctaaacaa tggtagcgtc caaagaaaat caatcgatgg
    19021 tgagtaataa tccaatcttt ctttggaatt ttagccatca aatctgcttc aatctgcttg
    19081 acatcggcat ctggtgatga aatattgagc ctctttgaga ctctagccac atgagtatca
    19141 accgcaatag ctggaacacc ataaacctct gccaatacca cattggctgt ttttcggcca
    19201 acacccggta aactttcaag ctctttgtgg gtttttggca cttgaccctt aaaatcatca
    19261 cgaatagcct gagcagtttt aatgatattt ttggccttat ttttatagag tccaatcgtt
    19321 ctcagagcat tttcaacatc agaaagttca gcaaaagcta agtcttctat ttctggataa
    19381 gactgccata gtccaggagt taccttattg acggccttat ctgtcgtttg agctgataaa
    19441 atcaccgcaa ttaacaattg aaaaggtgtt tcccagtcta actctccctt agcctcagga
    19501 aacatttgac caatgatggt caggactttt gctaatcttg cttttccaat tctcatgaat
    19561 cactccataa attcatcgca gatagaaaat cctctgatat cgtcacttga gaaagatctt
    19621 cgccttctct aacacgtcta cgttcttcta cttgtcgaag attaacaata ccttcttttc
    19681 gccaattacg taagatagcc tggatatatt tccaatttgt tttgccgttg aagacagctt
    19741 cttttaaagc ttctcgtatc aaatcaggat ctgttttatc atcacgaagc gttttttcaa
    19801 gatcttctaa ctcaaaagga ctcaagaatc gtcctaattc acgttcaaat tcatcaacta
    19861 atcgtttgaa atggtttggt gtttcttgtt tgtcaatttc agttgccgtt tgactgacaa
    19921 acaattgatc taacttggct aacacaggac tcgcatcaaa aataatttca atttctccag
    19981 taagttcaat tgtccgcata tctaaaagcc cttgactagt caaagatgaa atgattttat
    20041 taatatcagc aactgatttt ccaagcgcat gagcaatttg actgggcgct aagtcgtctc
    20101 gctttgtcgt attttgaaga tagaaaaact gccagaccaa aaaatcatct gagctcttaa
    20161 aaagatcttt gtaatgaaaa agcaaggcac ttgggataac caaattacct gacttataat
    20221 gttctaaaaa actcataatc cctcaatcaa aaggctaaaa tcgaatctat agcctttact
    20281 actttaaatc ccctaatcag atgctgatta atgtgcttac ccaggaaact gcattaagac
    20341 tttatagtca tagttcctaa tagcatgacg accatttaaa ccttctaatt caataaggaa
    20401 ggcacaacca gctacaatac caccaagttt ttcaatcatt tcaatagttg cttttaccgt
    20461 accgccagtc gctagtaaat catcgacaat caaaacacgt tgtccaggct taatagcatc
    20521 tgcatgcata gttaaggtat caagaccata ttctttttcg taatcggcag agacaacatc
    20581 tcttggcagt tttcctggtt tacgaacggg agcaaaacca attcctaact caactgcaac
    20641 aggacaacca atgataaaac cacgcgcttc aggtccaaca accatatcaa tatctttatc
    20701 acaagcgtac tgtgctattt cacgaatagc atagctatat gctttaccgt cagccattaa
    20761 aggtgagata tctcgaaacg taataccagc tttagggtaa tctttgatcg atgcaatgta
    20821 atttgttaaa tccatagtta attctttcta gtggcaaatt gccaaatata aaatactctt
    20881 cattatatca cgaaacatat ttttcgtaaa ataagacttc tgacattatg tttatctgat
    20941 acttcattcg taactacctc aataactact gtcaactgct ttacttattg atcgtttgat
    21001 ttcaccaaat aatcatacat ctctttcggg ctagccaaag ccatgatttc ttgaaatttc
    21061 accaattctt ttaagtcttg gtaaatgtgg ctctctgaaa tctctcgttt ttgcgcttgg
    21121 gggttaaccg tcatcagccc atcgtcaatc gtcacaaaag acaactcttc aaaaatttga
    21181 atcaacttaa tcagtaaaag tttttctatg tttaaatagt gactcaactc tgtcaattta
    21241 tgccgtaaat caaattcagg aaactgatag atggttttgt agagcttggc aaactgctct
    21301 ctgctgccaa atcctgttaa ataataagga tgtttcatct gatttttaaa atagatggcg
    21361 tcaaaatcct tgtgaacaaa ctggttgcgc caggttttga aatcttcagg gatgtcatta
    21421 atcagaatga ctctggcatt tgggtcttct tcaatagtcg gaataccttc aggaactttg
    21481 gctgtttttg tccttaagtc aagaagttgc actccgtcaa cacgagcatc tactaacatg
    21541 aattggagac tcgtgttccc attccaatgg ttgacagata aggtaactgc taattctaaa
    21601 ccagtagctt gtcggaactc ttgcagttga cttccttgac caaaagccag tacgtcaaag
    21661 gaagctttgc cctgactgac tttaaattta agatggctct ggtcttgccc tatggtacgc
    21721 gcttgactaa cccttatgtc cttgacataa aagaccggct tttgatgatc catcccataa
    21781 ggggctaatt tatctaaact ctttaaaata tcaaggctaa ggtcgtccaa agacagtctt
    21841 tcatcaatag ttaacgtatt tttggcagtc tgatctaagc cacgttctat cacaaactga
    21901 caaagaaagt ctgaaagagc ttccaagttg tcaacgggca gggtcattcc tgctgcacca
    21961 gcatgtcccc caaaggctgt aaataactca cgcttgccat tcaaggcttc aaaaatatta
    22021 atagcttcta aacttctggc cgagcctttt gcaaagccat tatcaatggt caaaacgacg
    22081 accgtttgtc caatagtttc catgatacga ccagcgacaa tcccaagaac accaggatgc
    22141 cagccagctt gtgccaagac ttgaacaggt ttttgaggat caaccatagc catcgcttga
    22201 tcaaaaatgt cttggactaa ggccttacgc tcttcatttt ttttcttaat catcaaggca
    22261 atcgcttgag cttcttggtc atcaaagccc gtcagtagct caatggctgg attagggtca
    22321 tctaagcgcc ctaaagcatt taattgcgga gcaatctgaa agccaatagc gtcttcatta
    22381 aaatgttcca aatcaatagg agacaaagac atcaactcct gcaagcccac tctttcggtg
    22441 tgtttgagca tggccagacc atttttgact aaaacacggt tttcacctgt caagcttacc
    22501 atatcggcaa tggttccaat agctaccaag tctagacagt ctgttggtaa actttctagc
    22561 aaagctgttg ccaatttaaa ggcaacgcca cagccagcta agtgtttaaa gggataatca
    22621 gcatcaggat gctcaggatg aatgatggca aaggcttctg gcaattcttc aggcaggcta
    22681 tgatggtcag ttacaatgac atcaacttcc tgttcttgag catatctaat cgcttcatgt
    22741 ccggccacgc cattatcaac cgtgataatt agggacacag cttcctgctc aataaaatat
    22801 ttatagacgc tctggttggg gccatagcca tctgtaaagc ggttgggaag ataaaccaaa
    22861 ggctctgctc ccatcatgtc tagggtttct ttgacaatag aagccgacgt catgccatca
    22921 gcatcatagt cgccgtaaat gagaattcgt tcgccctctt caatagcttg acgaattctt
    22981 ggcacagctt ttgccatgtc atgtagcaag taaggatcat gcagttgaga caagtccgct
    23041 gttaaaaact catctaaggc ttcttcagtc cttatccctc gctcataaat caactgagcg
    23101 gcggtctggg ttagaccttt tgtctttgct agcttaaaga agccatcatc tggcttcttg
    23161 tcttttattt tccagctata tttggattta atcatttata attaaatacc tttcttgcaa
    23221 gataatcaga taatttaggg aaaagggtgt aaaattgatg ggtcaccgct aaactaaagg
    23281 gcaaattcaa ttctcgttta tttttcccga taatagaaac caaacgctta gccacttgat
    23341 ttggttggag agtaaatgta ccaacgcttt ccaaataatg tccagacggg tcagcttggt
    23401 caaaaaactt ggtggcaatg ggacctggat tcacggtggt cacgtaaacc cctttatccg
    23461 ctaattctaa gcgaagggca ttggaaaatc cgataagggc aaacttggtg gctgaataaa
    23521 tgctcgattt ggctgacgca atcaagcctg ccatggacac aatattaata aggtgacctt
    23581 gcccctgctc tgccattttc tgaccaatca agcaagcaaa gtgaatgctc gctagggtgt
    23641 taacctgaaa catatcagct atttcttggg cagaaaactc ttcaaagcct ttgaaagctc
    23701 cgtagccagc attattaatc aagacatcaa tacggccata gcgctggtaa atctgggcga
    23761 ccattttctc aatggctact ggattggtaa tatccaactc aaggcattct ttgttgtcaa
    23821 tatgctgata acagtgttct aggcgttctt tgttacgtcc taataaaatc aagctgtctt
    23881 ccttgggtaa ctgcttaaca attgcctgag ccagtcctcc agaagctccc gtgataacaa
    23941 tgattctttg tgccattaaa taatcacttc ctctaaatct tgtaccattt taacattttc
    24001 aaatatagtg gcggcatctt tttccatctg gcgacaatca cgccctaaaa agcgagctga
    24061 tacatgattg agcaagaggc gttttgcgcc tgcttcatga gcgatttgtg ctgcctgcat
    24121 gttggtagaa tgcccgtgat ttctcgcaat ccgctcgtca cccttaccat aggtggattc
    24181 atgaaccaaa acatctgcat ctttagcgag tttaacactg gcagacgttt tgcgcgtatc
    24241 gccgataatg gtgatgattt tgcctttttt aggggcagaa atataatctt tggcacaaat
    24301 caggcggccg tcttccaact caacatcttg accattttta attttgccaa agagtggtcc
    24361 gaagggaaca cccgctgctt ttaaggcttc tgcgtctagg gttccttcta agtctttttg
    24421 aactactcga taacccatgc aaaaaatggt atgcgccaga cgctctgcat agaccacaaa
    24481 cttatctgtt tccattattt tacctaatga tttgtcatca aattcatgaa aatgaatctg
    24541 ataaggtaca cgcgctccag aaacttttag actagttaac acgtaagttt taatgccaat
    24601 tggtccataa atatcaagat ctgtctgttc ttcactagct tgaaaagacc gacttgataa
    24661 aaatcctggt aatccaaaaa tgtggtcccc atgcaaatga gtaatgaaaa tctttcttat
    24721 ttttcgtggc ttaatcgtcg tttctaaaat ttgtctttgc gttccttcac cgcaatcaaa
    24781 catccagact tcattgattt cgtctagtaa tttcaaggca agacttgaca cattgcgctg
    24841 cttggctggt tgaccagctc cagttcctaa aaattgtaat tccattcaat ctattctttc
    24901 tattgggtga tgtaaatcat aaattctcgt tgggcaaaat cataatactt ttgaccagaa
    24961 aatgtttggc taagagccca taactcctct acattaaacc ccttaagcat ggtgataccg
    25021 tttgccagac ggtaaatctt cgacaagagt gtgtcatcag gcgtaatgtc ttcacattct
    25081 ccttgctcaa actgcttagc ctgcatttta agctggcctt tgtcttcata aaccatatat
    25141 tgtgggaaaa tttgtgttaa ttcaaagaag agttcgggtt tcttaggctc tgctttttca
    25201 gaaaatacca atgcaacttc ttggtcattt tggtaagcaa aaccataagc ttttccagac
    25261 aaattgaggc gtacaaaggg cttttcttcc tcaaaagatg gttggtcagt aaaaagcccc
    25321 aaagccttag aaagattaaa ataataatca gtcgcagctt gcggtgactg tttctggtag
    25381 atttcagcaa agtaggcatt aataacactt ggtggtggtg tgataaaagc tagtttttgc
    25441 tggtctgtca aactggctaa tagatggttt aggtagttgg tatccagact agtaaatcca
    25501 ccataggttt ttgctaaatc aatataatta ttcataaaaa tcgtcaagtc tccattgctg
    25561 ttttggggaa atgtagcccg aaatatcctc tatttcttgg tcaaacgtgt agtggtctaa
    25621 aagagcaact cggttcaaat catacagctt gtacgccttg tcttgatgca ctttgattct
    25681 aaaaggaaca aactggtctc gaatttggtc aatgattaaa cgtctcaaga gaattttgct
    25741 atcctttgaa cgggctgaaa tcctaatatt tggaaaagca gtagctgtga attgttctgc
    25801 tatatccacc ttgttataaa tagctaaacg tggaatatta agcatgtcta gctcttttag
    25861 aaggtttaaa accacttttt cttgctcact atgattaggg tcgctagcgt caatcacatg
    25921 taacagcaaa tccacatatt tgctttcttc tagggttgat ttaaaagcag ccactaattc
    25981 cgttggcaag tcttgaataa agcctacggt gtctgttaag gttgcttgaa attgattttc
    26041 taaatagagt tgctttgtag ttgcatctaa ggtagcaaat aattcattgg cttcatagtg
    26101 gctgtcatct gtcaacaagt tcataatggt tgacttgcca gcattggtgt agccaatcaa
    26161 gccaatttta aaggtatctg aacccacacg acgatctcta attgtttgac gatttttctc
    26221 tacctgagtt aattggcgtt caatatcagc aatctgatga cgaatcgaac ggcgatttaa
    26281 ctctagttgg ctttcacctg gtccacggct cccaatacca cctgcttgac gacttaacat
    26341 gatgccctgt ccaaccaaac gaggtaacat atatttcaat tgggcaaggt gtacttgtaa
    26401 tttgccctca tggcttctgg cacgcatggc aaaaatatct aagattaact gcatacggtc
    26461 aatgactttt acctctaaaa cagcttctag attagcattt tgcctagcag tcaaccgatt
    26521 gttcacaata actgcatcaa tctcatcagc ttccacaatc gccttaatct catccagttt
    26581 ccctgagccg ataaaggttt tgctgtcgta acgttctcgt ttttggctga aacttgccat
    26641 aactttgacg ccagctgttt tggcaagatt agctaattct gtcatggaca tatcaaaatg
    26701 ttctgttgtc tgaagctcaa ctcctaaaag aatcactctt tcttgctgac gctttgtctc
    26761 aatcatcacc tttaccaatc ctccaaattc cgtaaattct attataacat atcttcaaca
    26821 gattctgcta attttgcgtc atgacttctc tttctgacca agaaaatccc tgaccctatc
    26881 atgtactacc tgtgggtaat caggagccgt tatggcagtg aagctaacag ccattcgatt
    26941 gcggaaccaa gttagctgtc gcttggcaaa acgtctcgtg ttttgtttta actgatcact
    27001 agcttctgcc aaagtcattt cacctacaaa ataggggaat aattccttat aacctatgcc
    27061 acgactagcc tgaacagtcg ggtaatgctc atataaccat tttgcttctt ccaataagcc
    27121 gttttcaagc atacgatcca cgcgctgatt gatacggtca tagatgactt ggcggtcatc
    27181 attcaagccg ataataagcg gctcataagc cgtctcggca ttctctaatt catcagcaaa
    27241 ctgagccaat tcaagggctc ggatcgcccg cctgcgattc acctgctcaa tggtaatgtt
    27301 attgacttgc aagcgttcat acaagtcgtg atcgtccaat tgctccaact cattccgata
    27361 ggcttttacc gcctcttgat caacttgacc tcctagatga taaccttcga gtaaactttg
    27421 taagtaaaga ccagttccac ctacaataat tggtaatttc ccacgactaa caatgtctga
    27481 aatagctttt tgagcatctt gaacaaaatc gtaggccgaa taagactccg tcacctcacg
    27541 aatatcagtt aagtgatgaa ccgctgcttc ttgctcttct tgagtggcct tggctgttcc
    27601 aatatccaac tgtcgataaa cttgctggct atctcctgaa ataatttctc cattaaaagc
    27661 cttggctaaa ctaatcccaa gtgctgtttt tccaaccgct gtcggaccaa caatcacaac
    27721 tatttttatt tttgtcattt tcttgtcttt ctattagaaa agcgtgtcca aaaacgcctt
    27781 ttacttgaaa attagcttat ttttcgctac catatattat aacataagat taaggaccac
    27841 tgaggtcctt cactagaaat ggagaataat catggaatat caatttgtta aaggtcttgc
    27901 tactggcgtc ctagcaactg ctgctactgt tgctggtgct gtctttgctg taaaaaaaac
    27961 aatcattgac cctgaagaag aaaaagctgc tttcattgaa gaaaaccgca aaaaagctgc
    28021 gcgtcgtcgt gtagctcgct aacgtgacaa tggatcaatt taaaagagac tagcacatta
    28081 aagtgccggc ctctttttta gtaatctcga taactagtta gtagtaactg tctcttgatg
    28141 agccaagatg ttagctgctt tacttatttt gaaatagaaa tctcaggtaa gccccataaa
    28201 catacgtgac taaagctgtc gccaacaata cctctcctag caaaagaacc tgccaaccta
    28261 aaccttgcca acctaaaaaa gataaggtca tttttaagga agtggcacta atcacaaacg
    28321 gaaaggtaaa agctgaaaaa cctgggttaa agggacgatt gagtaagcga ggaagctgta
    28381 caaccacaaa agcatataac agttgtgagc tgatgagaag aaagataacc attcctactt
    28441 gtggtctagg aaaggttgct aaataagacg ctgaaagtaa ggacagaggg gcacaaaacg
    28501 ttgaaatatt gggcattact gcttgaccta aaccaatccc ataagtcttt ttggccataa
    28561 aaggtaaaat cacagcagtt aagagaagac aaacccaaaa aatcacctgt cctaataaaa
    28621 actgcctact agcaggtgct gttaatgccg ccattgcaat tccaacaaaa agcacggacc
    28681 aggaaggaaa aacgttatcc catgaaaaag aaaagacaaa acgatactgg taataagcaa
    28741 tcaggacaag atttcccaaa aagaatagcc accaagtgag aaagcctaac caacaccctc
    28801 ctgtcacttt gagaaagagt gaagacaaca acatccctgt cataaaaaaa gttggaaaaa
    28861 cagaggcaat tagaggttgt cgtaactgca ttttggtatc gttaaggttc ctaaccatgc
    28921 caacaagcaa gataccataa ataaaaagag ctgccagaat tcccaaataa ctaaaaatcg
    28981 aaacataagt agccaacaaa ttgccaaatg acaacgttcc taatgccaat cctgacatga
    29041 ccaatggtgg gtttttaagg tgtttcataa gtgtcctttt ctgcctatta acagctattt
    29101 ttagtgacat aatcttgttt attttatcac atttcgagca tttttgccat aaaggctgcc
    29161 agatgcggat aattctcgta agattttggt gttttttgag accatagcgc catggcgtaa
    29221 gcataagcat ctgcaatggt ttgttgatct cgataaacat gcccactatc cccaataaga
    29281 ccatctaaat gcgtcatagc aatatccatc cgcttgtaag aagcttgttt gactttttca
    29341 agcgcttgat tgtcttgaga aatagtgtag cgatgagggt tgtacattgg cctaaaaaca
    29401 aggggaaaat cacctgttaa gaaatccatg atttcgttga atcgactttc tgccaaaaga
    29461 ttatcttggg gaatgagctg agatttcagg gtttgtttcg ctataaagag caagataata
    29521 cctgcctgtg tgacgatttg accatcatca agctgtaaag cttaaaccat tcccaaagga
    29581 ttgatggtaa ggcagtcttc agaatccagt tttaccttct ctatctcgta atacagaaac
    29641 tcacaattct tttgaaaagg ttctagacag agttggtgtt gacgtcttcg ctgttgatgg
    29701 ctctgcatga caaaatgagc ttggtgtcca cgttcactag tatcttgttg caaagtcttt
    29761 gcaatttggt ctcatcatct gtatcacaaa aaacaaacca gtttcctgat ttgttttgag
    29821 atattaggca cctacaaatt ctggcaagga ttctccaata gcttctaaac gttctgccca
    29881 tccttgacgt gataaaccat tttcttgcac atagcgatta cgttcatgtg cacgacattc
    29941 aggcgagcat cctcgaacat actttgtttc gttttcttct gaagcaaaaa tttgtttgtt
    30001 acaaaacgga tttgcacaat tgacataacg ttcacaaggt gttccatcaa aataatcttt
    30061 actaatgaca gttggattaa catgattaat tggcaccgag atacgatcat caaaaacata
    30121 catcgcacca tcccaaagtt caccttgaac ttccggatct tttccatatg ttgcaatacc
    30181 accgtgtaat tgtccaacat ctttaaagcc ttctcgaacc atccaacccg agaatttctc
    30241 acagcgaaca ccacccgtac agtaaacaac aacacgtttt tccataaact tatctttgtt
    30301 gtcacgtacc cattgtggta actcacggaa attgcgaata tcaggacgaa ttgctccacg
    30361 gaagtgacct aagtcatatt cataatcatt acgcgtatct aaaacaacag tatcttcatc
    30421 aagcaaggct tctttaaatt gtttaggatt taaatactca cctgttgttt ccaaaggatt
    30481 aatatcacta tcaaagttat tgtcttctaa cccaaggtgc acgatctctt tcttataacg
    30541 aacaaacatt ttacggaaag cttgttgatt ttcctcgtca atcttgaacc aaagatcagc
    30601 aaaacgctca tcgctgtgga cccaatccat atacttctga gttgtttcat aatctccaga
    30661 aacagttcca ttaatcccct catccgcaat caaaatacga cctttaagcc caatagattt
    30721 acaaaattct agatgtttcg cagcgtactc ttgcgcattt tcaattgaaa catatttata
    30781 atagagtaaa actctgattt tttctgacat tttttctcct tcattgttat tcagttactt
    30841 cctattatat ctaggaaaaa caagtctagg caagtcattt gtttgtcttt tttttcacaa
    30901 aatagatctc aatttatttt tgatgagatg agatgcaacc cattactcct ttaagcagat
    30961 cattagaagc tttatttaga aaatgctaag ataaaacaag gaggccaaaa tgacttatga
    31021 actttgttta gaatatggga cttatccgtt gtcacgtgtt tatgcttatt ggggagaaga
    31081 tcaaaatccc ccaacattca ttcaagaaga tcgactgctt tgccataaac tagaaactat
    31141 gaatcacctg ttccatgatt tatttgtaac tattgaatca caattccatt atgttggttt
    31201 taacatgcct gaaaaaagag cgcagattcg gatactatat caagaagtag ctactatatt
    31261 aaaatccaaa tataaggatt atccaattaa gattgagacg tttcttcttt aaaaatctaa
    31321 tactaaaaaa ctaactgtgc ttagacagat gacacagcta gttttttagg taaaactctt
    31381 cttaaatttt aaaacgtttc gaaaaatcac taaatgcttt aatagcactt gctaaaaatg
    31441 gtttcaccat ctcatcaata attaaccttg attacttaca atcttatgaa tctgagtcaa
    31501 ataaaattca ggctgtggca ttacagagat atttaaaacc tatactcatc gttttcaagg
    31561 tcagaaaaat aatgattttg aaaaggttcg tcaactcttc tagataattt tggacaaatt
    31621 ttggaaaaga aacaaaaaaa gcctatcaaa tagggctttt ttatatgatt tctatccacc
    31681 ttgagggaat cgaaccccca tctcaagaac cggaatctta cgtgatatcc attacactaa
    31741 aggtggtcac aactttgtca atacccttat agtatatcat aaaatgaaat aatatgaaat
    31801 aacttttcta accagaagac aataaaaagt tgaggaaaac ctcaactttt tagatttatt
    31861 acaattcaat atcaccgaaa agatcagcca ttgagaatcc agtttgtgtt tctgggagtt
    31921 cgtagtcacg tcttgattca cgttttggac gacgtgggcg tgattgacgt ttttcttctt
    31981 tgttgtctcc ttcagcttgc gctgggcgtt cttcaagagc tttgattgat aatgatacgc
    32041 gctcatcagc tgcattcact tcaagaactt taactgttac ttcttgtcct acagaaagta
    32101 catcttttgg attttcaaca cgtttgtgtg aaatttgtga aatatgaaca agtccatcaa
    32161 tacctggtaa tacttcaaca aaagcaccga agtcagtcaa gcgttttact ttaccttcaa
    32221 caacatcacc ttgagcaagt ttttgttcaa cgccgtccca tggtccaggt gttgttgctt
    32281 taagtgaaag tgacacacga ccagcttctt cgtcaattga aagaacttta acttcaactt
    32341 cttcaccaac tgaaacaact gatttaggtg acacattacg ttcgtgagac aattcagtca
    32401 cgtgaacaag tccgtcaaca ccaccaaggt caatgaaagc accaaaactt gttaaacgcg
    32461 ctacagtacc tgtaacgact gcaccttctg aaatcttaga gaacacttca gcacgagctt
    32521 ctttagcggc ctcttcgata acttcacgac gtgataggat gaaacggttt tctgctgcat
    32581 caacttcttt gattttagca tcaaactctt gtccaacaaa tttttcagtg ttgcgaacaa
    32641 aacgagtgtc aatcattgaa gcagggataa atccacgaag tccttcaaat tcaactgaaa
    32701 gaccaccttt aacagcacgg gtacctttaa cagtaacaac ttcgccttca cgaccaacaa
    32761 gtttgtccca ggctttacga gcttccaaac gttttttaga tactaggaaa gtaactgtat
    32821 cagtatcttt acctactact tgacgaagaa caagtacttc aactgtgtcg ccagctttaa
    32881 caaagtcgtt aatatcagca tcgcggtcgt tagtcaattc acgaagtgtc aagacacctt
    32941 caacacctgt tccttcgata acaacgtttg cttgaccgtt atcaacagtt aaaacttccg
    33001 cagtgacaac atcaccaggg ttcacttcgc taacactgtt tagcaaatct tcaaattcat
    33061 tcattctaaa aaatcctcca acaaaagcta gccaatgcta gcttttgata acaatatatt
    33121 ttctcaaggt aacccgcaac gacaacaact cttatctttg acggtctcag ggagcacctg
    33181 atacctctga ctggggtagc tggattcgaa ccaacgcatg agggagtcaa agtcccttgc
    33241 cttaccgctt ggctataccc caaaaatatt ttttacagaa aactaatttt ctaattagat
    33301 tagtttccga aatggaaaga gagggattcg aacccccgaa cccgaaggag cggatttaca
    33361 gtccgccgcg tttagcctct tcgctatctt tccgtaacaa caaaaactat tctatcatag
    33421 tttttgtaag ctggcaagtg tttttgtcta attttaatgg ttcaaattgt agttttcaat
    33481 gatattttca accgcttgtt cgagcgtttt gaagaagcaa tctactccat cattttttaa
    33541 gacagcaaat ttctcatcaa gttctgcaat ttctccaacc acacgtttac caatttgtaa
    33601 ttgatagcca tcaatcttag tttcattaac cataactttg tgatcaatca gttggatttc
    33661 aattttttta tcttttttac tcatcacttt acctcacttt gtctatttta cacaaaatca
    33721 aaaaagcttg caagagcaag ctctatttta atctactttt acaatccaac cttctggtgc
    33781 ttcaatatcc ccaaattgaa taccgaccaa ctcatcgtat aaacggcgtg tcacaggacc
    33841 aacttctgtt tcactgtaaa agacatgtaa gttatccttg tactggatac caccaattgg
    33901 agagataaca gctgctgttc cacaagctcc tgcttccaca aatttatcca attcattaat
    33961 tggaacatca ccctcaatta ctgtcatacc aagtctttct tcagctagtt gtaagagtga
    34021 atacttcgta atagatggca agatagatgg actaagtggc gtaacaaatt cattatttgc
    34081 agtaatgcca aaaaagtttg cagctccgac ttcttcaatt ttagtatggg ttgctggatc
    34141 taagtaaatc acatcagaaa agcctgctga tttagcggcc ttacctggta gcaaacttcc
    34201 tgcatagttt cctcccacct tagcagcacc tgttccgtaa ggagcggcgc ggtcaaacgc
    34261 ttcagaaaca ataaaattag tcggagcaag tccacctttg aaatagtttc ctactggcat
    34321 ggcaaaaatg gtgaaaatat attcttcggc tggtttaacc ccaataatat ctccaactcc
    34381 aattaataac ggtctcaggt aaagcgttgc acctgttccg taaggtggta caaagtcttc
    34441 attggcttta accacttgct tagctgcatc aataaattgc tctgttgata cttgtggcat
    34501 caacaagcgg tctgctgtgg cttgtaaacg cactgcatta cggtctggtc taaatagttg
    34561 aatagagcca tctttggtgc gataagcctt taaaccttca aaagcttgtt gcccataatg
    34621 aagcgctgga gcgctttctg agatatgtaa ggtggcatct tctgttaatt gacctttatc
    34681 ccattgacca tttttgtaat aagaaatgta acgaaatggc aacttatgat attcaaatcc
    34741 taggttgtcc caatcaattg ctattgtcat catcgtacct cttttctgct tttttctatt
    34801 gtaacttttt ttcaataatt ttcaagttta ttctactctt ttgcattaat attctaaatt
    34861 taccataaaa ttaaaaagag ttttcactct ttttaacgtg ttctagctac cagtacttct
    34921 tgatccgaaa tagtgtctga aataaaggag ccattgctgg ttctttccga taacgaaagc
    34981 gtttctaaag caatctcgta ggtttgacca gtcttactaa tgacttcaag catttgttga
    35041 ttagtttctt cttcaggtat atcagtgaac aaatcaaact gctcagctga agtatctgat
    35101 tgaacgcctc cggccagaaa aactctatga ggtttggttt ttaactctcg taggaccaat
    35161 agtccacgat tggcacgact tgtctgagga atatcatcga cagccatccg tttgagagaa
    35221 cccctctgag ttaagacaaa gaaagagtta cttgtcacag caaaggctga ggcaaggctg
    35281 tcatcatttt tcaagttaat gcctttaaca ccagcagatt tcaacccttg aataggcact
    35341 tcctgactgg agaatcgcaa agcataacca tttttagtca ctaataggag gtcttccata
    35401 ataactggtg acaaggtaac cacgcggtct ttgtcatcct ttagcttcac atatttggtt
    35461 gatttagatt tataagttcg ccaaggactt agttctttac gatcaaagcg tttgataaaa
    35521 ccattttgtg tgacagccac atacaagccc tggtcaaagg aagtcacaat atcagcgtag
    35581 agaatctctt cttctgtcgc gaagtttgaa atggtttgtg ataaatgttc accgatatcc
    35641 ttccaacgta aatcggtcaa ttcatggatt ggccggtaaa tgacattgcc taaagttgta
    35701 aacaacaaaa ggtgttctgt tgttttggcc tgtttaacga agatgagttc gtcatcatct
    35761 ctcttgccaa cttcttccaa actagacgca ttgaaggatc gtgggctggt tcttttgagg
    35821 tatccgcccc gtgtcacact aacaaaagtt tcttcctctg caatcaagct ggcagtgtca
    35881 atctcaatga tttgactttc tgcttgtaac tctgataaac gaggattggc gaatttcttt
    35941 ttcacttctc ttaattcacg tttcatgacg ttatacatgg ttgcttcgtc accgataata
    36001 gcggaaaggg tcgtgattaa gtctcttaaa tcattttctt cattttggag agtaacaata
    36061 tcggtattag ttaaacgata aagctgcaag gttacaatcg cttctgcttg ttcctcagag
    36121 aaatcatagc tgactttcag attttcttta gcatcagtct tattgtcaga cgatctaatc
    36181 aaagcaataa tctcatctaa gatggacaag acacgaatca atccttcaac aatgtggagg
    36241 cgtttttcag ctttggcttt atcgaattta gaccgctcaa taataatatc tttgcggtga
    36301 ctgatatagc ttgatagtat tttttgaagc cctacttgac gtggtgtgaa gtgatcaatg
    36361 gcaaccatat tgaaattata attaacttga aggtctgtgt atttcaacaa ataatttaga
    36421 atggtttggc tatctgcatc ttttttcaac tcaatagcta tgcgaagacc tgtgcgatca
    36481 gattcatcac gtacttctac gatgcctggg actttattgt taacacgaac atcgtcaatc
    36541 tttttgacca agactgcttt gttaacttca taaggaatct cagtgacaat aatttgttgt
    36601 ttaccacctt taagttcctc aatttccgtc ctagagcgaa caacgacacg tcccttacct
    36661 gtttcgtagg cttttttgat ttcgtcagct ccttggataa tgcctcctgt cgggaaatct
    36721 ggcccaggca aaaattccat caatttctct aagctagcct tgggatgatc aatcatatac
    36781 acaacagcat caatcacttc tgacaaattg tgcggtggaa tatctgtcgc atagcctgcg
    36841 gaaataccag aagacccatt gacaagtaag ttaggaaaag ctgctggtaa aacggtgggt
    36901 tctttttctg tgtcatcaaa gttccacgca aaagagaccg tatttttttc aatgtcttga
    36961 agaagataac ctgcaatttc agacaagcga gcttctgtat aacgcatagc cgcaggcgga
    37021 tcgccatcca ttgaaccatt atttccgtgc atttcaacaa ggatttcacg gtttttccaa
    37081 ttttgactca tccgtaccat ggcatcataa atagaggaat ccccatgcgg gtggaaattc
    37141 cccatgatat taccaactga tttggctgat ttacggtaac ctttttcaaa agtattgcca
    37201 tccttattca tcgaataaag aatccgacgc tgaacgggtt tcaatccatc tcgaatatca
    37261 ggaagtgccc gttcctgaat aatatacttg gaataacggc cgaagcgttc tcccataata
    37321 tcctcaaggg acatgttttg aatgtttgac atattcaaat agctttcttt ttcattattt
    37381 attaattacc taaaaaacaa tcgtctttag cacttgtaat tatctgtgag aaacttgtat
    37441 gactaaaaca ccgtattttc ttctaaggta aacttgacgt tatcctcgat ccactgacgg
    37501 cgtggggctg ctttgtcacc catcaacaca gaaacacgtc gctctgctct tgccaagtcg
    37561 tcaatagtaa cacgaataag ggttctggtt tcagggtcca tagtggtttc ccataattgg
    37621 ttagcattca tttcacccag tcctttatag cgttgaagaa tagctccttt accaaattcc
    37681 ttacgtaaat cttctaattc accatctgtc caagcgtagg acactttctc tgttttacct
    37741 ttaccttttg acattttata aagcggggga agcgcaatat aaacgtgacc tgcttccacc
    37801 aacggtcgca tataacggta aaagaaagtc agcaaaagag tctgaatatg agccccatcg
    37861 gtatcagcat cggtcatgat aataatctta tcatagttga tatcctcgag gttaaagtca
    37921 gctcccactc cagctccaat ggtataaacc atagtgttaa tttcttcatt tttcaagatg
    37981 tcagccatct tagctttttc ggtatttaaa actttgccac gtaatggtaa aatagcctga
    38041 aatttgcggt ctcgtccttg tttggctgaa cctcccgcag aatctccttc gaccagatac
    38101 aattcatttt tcttggcatt tttggactgg gctggggtta attttcccga caacaagcct
    38161 ttgtcttttt tgtttttctt accattacgt gagtcatcac gcgcttttcg agcagcttcg
    38221 cgagcatctc tagctttaat ggctttgcga actagatgag aagctacttc tccattttcg
    38281 agtaaaaaga aggttaattt ttcagaaaca atgctctcta caataggtct cgctaaggga
    38341 cttcccaatt tgtctttggt ctgtccttca aattgcaagt gctgctcagg aactaagata
    38401 gataatactg ctgagagtcc ttctcgataa tctgagccct ctagattttt atccttttct
    38461 tttaagagat tggttttacg agcatagtca ttcatcgctt tggtaatagc tgactttagt
    38521 cccgtttcat ggcttccacc atctttggtg cgcacattgt ttacaaagga taagatattg
    38581 tcggagaaac catcgttata ctgcaaagca acctcgactt gaaagtcttg gtcttgacct
    38641 tccatgtaaa taacaggtgt caaggtttcc ttatcttcat tgaggtattc gacgaaatct
    38701 tgaaccccat tttcataatg aaactcttca ataatcgggt catctccacg aagatctgtc
    38761 aagctcatct tgacattttt caaaagaaaa gcagattctt tcaatcgttc tgaaatggta
    38821 ttgaacttaa agtcaatagt tgaaaaaatc ttatcatctg gcataaaggt tacaaccgtt
    38881 cctgatttag acttaggagc tgtgccaact tttttaaggg ttgtaacagg tttcccccca
    38941 ttttcaaagc gctgacgata cactgacccg tctcttgtga tttcgacttc taaccaggca
    39001 gacaaagcat tgacaacaga ggagccaaca ccgtgcaaac cacctgacgt tttgtagcca
    39061 ccctgaccaa atttaccacc tgcatgaaga atcgtaaaaa taacttgaac ggtgggaatc
    39121 cccatggcat gctgacctgt tggcattcca cgtccgctat ctgccacact gaccgagcca
    39181 tctttattga taacaacctt aatatcatct ccaaaaccag acaaggcctc atcgacagca
    39241 ttatcaacaa tttcccaaat caggtggtgc aatcccgtag catctgttga accgatatac
    39301 atccctggac gcttgcgaac agcatctaac ccttctagga cttggatagc atcgtcatta
    39361 taattattaa ttgtaatttc tttcttagtc aacaccgacc tccagacagt ttcatctttt
    39421 ttatcttaca agttttttac caattttgca aaataatttg ctaatttttt ccgaattctt
    39481 ttccagatct agttctccaa tctatgaata tgttataata aggattatga aattactact
    39541 ttttattacc attgcctatt tacttggttc tattccaact ggactatgga ttggacagta
    39601 cttttaccac atcaacttac gagagcatgg atcaggaaat actggaacca caaatacttt
    39661 tcggatttta ggtgtcaagg caggaacagc taccttagct attgatatgt ttaaagggac
    39721 actttcaata ttgttaccaa ttatttttgg tatgacttca atttcatcca ttgctatcgg
    39781 ctttttcgca gttttagggc atacttttcc tatttttgcc aactttaaag gtggtaaggc
    39841 cgtagcaaca agtgctggtg tattgctagg ctttgctccg ttatatctct tttttttagc
    39901 atctatcttt gttttagttc tctatttatt tagcatgata tctttagcta gtgtggtttc
    39961 agctatcgtt ggtgtgttat ctgttttaac atttcctgcc attcattttc ttttaccaaa
    40021 ttatgactac tttttaactt ttattgtgat attacttgcg ttcattatta ttataagaca
    40081 caaagataac attagtcgta tcaaacatca tactgaaaat ctaatacctt gggggctaaa
    40141 tttaagcaaa caagtgccta aaaaataaat ttactaaaag cctgacttcc tctgaaagtc
    40201 aagctttttt gttttaactc atttgttaat tagggtaaac aacctctccg tctgcaatag
    40261 tgtatttcac gacacctttg agtttgttac caataaatgg ggaattacta gctttagaag
    40321 caaaattctc agtgatcaga cgttcttgct tgtctgcaaa aataaccaaa tctgctggac
    40381 cattttctgc caaataaccc gcatcaaaac cataaagcaa agctggatta agcgtcattt
    40441 tttctaatag tgacataagt gtaagatgac ctggttcaac caaatgcgtc aaacctaatg
    40501 acaaagaagt ttcaagtcct gtcataccag atggagcttt agtcatatca tcaacagctt
    40561 tttcatcctt gtgatgagga gcatgatcag tggcaatgac tgttataaca cccgatttca
    40621 agccttctat gacagctaag cgatctcttt gtgtacgaag gggaggattc atcttggcgc
    40681 tggtacctac aatcaataag agatcttcag ttgttgaaaa atgttgtggg gacacttcag
    40741 cagttacctt agcacccaac tgctgagcaa aagccactac ttgaaccgat tctgctttag
    40801 ataaatgttg gatatgaaca tgagcttgcc tgtcataagc aatcatgaca tctcttgcaa
    40861 tcatactata ttctgctaca ccagttgccc cacaaaaatg aaaatgctcc tcagcaatac
    40921 cttcattaaa accaagaacc ccatttaact gaggatcttc ctcatgcaaa gaaatgaaag
    40981 tttggttggc attagccaaa tcaaaggctt cttttaaaac tttagaactt tctaagggaa
    41041 taccatcatc tgaaaagctt actgctcctg cttccaacaa agctttaaaa tcagtcacgt
    41101 cttttccatt aaaggcttgg gtgacactgg cattggtgta aatatgaatc ttttctttag
    41161 ctgcgctagc taatacttcc tgtaaagttt cgacatctga aatgacagga ttggtattag
    41221 ccatcatgac aaccgtagtc acaccacctg atgctgctgc caaggctccc gtgtgaatat
    41281 cttctttgtg cgtttgaccg ggttctctaa aatgcacatg aatatcgacc aaacccggag
    41341 caacaataag accactagca tcaatcactt gagcttcttg gcattcaata gctgaagcta
    41401 tctttacaat ctgtttacca tcaatcaaga catctgccac ttgatctctc tgcgatttcg
    41461 ggtccataac gcgtccattt ttgatgagta acatgctctc tccttcttct cacttatctg
    41521 taaaactgat agacagacct gtctgccaat aatactgaaa ctttgtccat aggctgctta
    41581 cctcaaacca tctgcctgat aatcactaat gcttgtccta ttgtaaccaa tctatgccag
    41641 tcatgccttc tttttctaaa atagcatttg ttcttgaaaa gggtttgctg ccaaaaaagc
    41701 cacgataaga cgacaaaggg cttggatgag gactctcgat gatgtggtgc tttggattag
    41761 taatgaaggc ttttttcttc cttgcataag ctccccacaa aatgaaaact acagggctgt
    41821 ctttctcatt cagcacttta ataacagcgt ctgtaaatgg ctcccatatt aagcctgcat
    41881 gcccattagc ctggcctgct ggcactgtta agcaagcatt caaaagcaga accccttgac
    41941 tagcccaagt gcttaaatca tgatggtcac gaggaccaat gtcatctgct aattctttta
    42001 aaatattaat aagggatggc ggagcagaaa tctcttctgg aactgaaaat gataagccct
    42061 gggcctgttt ggggccatga taagggtctt gtcctaaaat caacactttg gtttcttcca
    42121 aaggagtgac ttgtaaggct ttaaagacat tttctcgtgg aggatagaca agaccggaag
    42181 cataggcttc atctaaaaag tgattgatgc gaccgtagta atgctccggc aaaaatgatt
    42241 tgattttctc atgccaaatt gaatgagcca taagattcct ttctaatcac gtcagtttct
    42301 aagttaaata cgatgtcctt ttctatctag tttaccacat tttaaataga aagaccctga
    42361 aaatatgata tttccagggt taatattcgt tttttacagg atattaaaca agtgctagct
    42421 tattttctaa gaattcttgg taacgttcta acaaaaaagc aataatccaa tagataaagg
    42481 ctactaaaat gtacatggac atgtagtccc actccctacc accgataatt ttggcatttt
    42541 gaaaaatatc tggtacggta atcatggcag ctagcgacga acttttaacc atatcaataa
    42601 tgacattgcc tagaggagga actgcaattc gaaaggcttg aggaagaata attttcttaa
    42661 ccataagagc atatggcaac cctaaagcct tagctgcttc ccactgtccc ttgtcaatag
    42721 cttctataga agagcgaaaa acttcagaaa tataggcagc gctaaccata gaaaaaccaa
    42781 gataagcgca aagtaaagct ggcaattcta aaccataata aggcaacccg aaataaagaa
    42841 caaagagcac cacgatcatc ggcacacctc gcataataga gatgtaggcc ctaacaagat
    42901 agtgtagcag tggctgatgt gaccttccta gtagggcaag gagcaaacca aagaccagcc
    42961 ccgttaaaaa gctaagcaac gatatgccaa gggtataagg taatcccgat aatacaaatc
    43021 ctaaactatc tttcattaat gggatattaa tcatgtggtt aggactcctt taacatcaag
    43081 tattagttaa cgtctttcgt atcaatgatg ggaatttttt tgtctttgcc aaaaggtttt
    43141 gtcaaatctt gaccagcata gtaggtttca gatattttct tcaagctacc atcttttatc
    43201 atgtctttaa taacagcatc tatttttttc ttgaggctat cgtctttttt gttcataaca
    43261 attccttgtt cggttgggtt gtactgagca tcgctcattt tgacatttag atttggattt
    43321 tgagataaca tgtaatccac aaataatttt tgagctgggt aatcatttgg aatgaaatct
    43381 gttcgaccgt tagccacatc gtttagataa acatctcctg taacattatc gtaggtcacc
    43441 agttcagctc cctgtttttg cgctactttc atgtactctg ttccagaagc gccagctgct
    43501 ttttttcctt tccagtcgct aagatctttt ttagccatgt ttgagctgcc atcagcacgg
    43561 acaatcatgc ctcctacccc gtatttataa ggcgtagaga tattgtattt tttctgacgt
    43621 tttggggtaa tgtcaaagtt gttcaaagaa atatccactc gaccactatc aactgaggta
    43681 aaggcttggt caaatcctgt ttcaacaaac ttgactttaa tgttaagtcg tttaccgatt
    43741 tccttgacca tatctacttc gtaacctact aactcattat tatcgttgta aaaagaagtt
    43801 ggctgatagg ttcctggggt agctactttt aacacccctt tttctttgat agtctcccaa
    43861 cttgattttt gagatttatg tggcgcacat gctgccaata aaaggctggc aagtccgata
    43921 gacagtaaag taactgattt cttcatagta actcctttat taagataaga atagtataac
    43981 attttgatta agttgtgtta tcagtgagcg ctcaatctct ttaaaaagtt aatctataat
    44041 agttaaactg ttttataaga ttattattga ggaatcttca ataatcctgc tgcctcaaac
    44101 tgctctgcaa ttcctaataa taggtcctct ctacctttgg ctgcaattag ttgaatgccc
    44161 attgggagcc cttccttagt ttcgtaagta ggtaaactaa tagctggctg gccagttaga
    44221 ttaggcagag ctgtgtaagg attaatagcc agactcttac caaacatggt ttcgaccaaa
    44281 ttgaattgct cttcactgct gaaaatttca gcttgagcca agttagccat gagtttcgaa
    44341 tctgggacaa gttctccgtg cttaggagct ggtgtgttgg ttgtgaacgt caacaacagg
    44401 tcataagtct catgaaaact agccatggtt gcactgtacg tatcccattt ttgaaggacc
    44461 tgtgagtaac gccaagctgg gatgtcttta cctgattggt aaatagccca ggtcatggtt
    44521 tccatatcgt ctttggtcat cggacgtcca aaagtgtctt caatatcagc aaacatagca
    44581 gccgtttcca cactattcat aatataataa tgacgaatca cttctgtcat atttacagga
    44641 aactcttcca attcaactag ctgatgccct tgttctctta accaagttac tgcttgtctt
    44701 aaagcttttt cagtatctaa agatactggg ctaccatcac ttaaccgttg ataaaaagca
    44761 attgtcaagg gacgttgtag cgattgataa atactatttt ttgttaaggt ggctaggggg
    44821 aaggggcttt ccatttgttc catctgtaaa tagtagagta gatttctagt atctctaacg
    44881 gatttggtca aggcaaaatg aactgaggct ccttgccagc ttcgataaga accaggtccg
    44941 acaggcatgc gcccgcggct aggtttgagc ccaattaagc cattaaacga ggctggaatt
    45001 cgaatagaac caccaccatc actagcagtt gctagtgcac taattcctga tgacactaga
    45061 gcagcagctc ccccagatga gccaccagca tttcgtgtat tatcatgagg taatgaaacc
    45121 gggccatgga gctgactatc actaatattt ttaaagccaa attcaggtgt gttagaacgc
    45181 cctaaaataa taaatcccaa gtcttctaac cttttcacga ataaatctgt tttagtcgca
    45241 tgatgattct tgaatagctt agaacccgat gttgataagt ctcccttcaa ttcttgtccc
    45301 aaatctttca aaaaaatggg aactcctgca aaaagcttac ctgaaaaatc cctttgtttg
    45361 gcttcttcta gggctgcttc aaagcgttct gaggtaatag catttaaggt agggttcaac
    45421 tttttagcct tataaatagc ctgggttact aattctagtg gtgtggtctg accggtctga
    45481 acagcaatag ccatagccgt cgcatcttga taagtcatag ctactcttcc tttctaagat
    45541 atgaatccta aaacattctt ttttattaaa acacaaataa aagaaaacca ccattaaaag
    45601 gttagaaatt agaatgtgat gtgtaaggaa tgtagacaaa acaatcaaaa cataacattt
    45661 tgaattgggg ttaaagagat tttttcatta aaaataaacc tggctgatag ttccttattg
    45721 ttgccagttt acctggtctt ctttaaattt tttaagtaaa tgtaaaccat cattagtaat
    45781 atagccttgc aactttgcaa ctgcaattag ctcagtataa ttagaaagcg taatcaactt
    45841 gataccagct tctttaaaat tctgactagc ttttggtaac tcataagtga aaatcgcaac
    45901 aactcccaga acgtcagctc cctcacgact ggctgctgcg gctgcatcta gtaccgaacc
    45961 acctgttgaa atcaaatctt caatgataac cattttttgg cctttaagca cacgcccctc
    46021 aatctggttg cctgctccat gatcttttgg cttactgcga atataagcaa aaggaagagt
    46081 cattttatct gctataatgg caccgtgagg aatacctgct gtagctgtac cagcgatgac
    46141 ctcaacttct gggaaatgag ccctgatagt ttccacaaac ccattctcaa ttaaatcacg
    46201 agtcttagga taagaaaggg tgacacgatt atcagtgtaa ataggtgatt taatgccaga
    46261 tgcccacgta aaaggatctt caggctttaa ataaacagct ttaatatcaa gtaattgagt
    46321 tgcaatttgg cttcctagtg tcatttctta ttctcctttt tcttctttaa cagacaagtg
    46381 ttggtttcct aatcttatct gttagcctgc ccactcagct tttatggctt ggtaagcagc
    46441 cactgggtct tttgcctggg taattggtcg ccctaccaca atataatcac ttccaatgcg
    46501 tcttgcttgg tttggagtca tcactcgttt ttgatcgcca atattactgc ctttaggacg
    46561 aatgccagga gttaagcaag taaatcccgt aggcgtcacc gccttgatag cttctacttc
    46621 ttgtgccgaa caaaccaccc catctagctg agctttagct gcccccttgc tgtaatgcaa
    46681 aacagactct aaaagactcg tttgaatatt ttgatcacca cgcatttggt cttcactggt
    46741 tgaagttagc tgcgttaccg caattaaggt agggccttgg cctagtcctt ctcgagctgc
    46801 ttttagcatc tctacaccac cagctgcatg aacagttgcc atatcaatat ctaattcttt
    46861 aagaactgcc atagcagcac gaacagtatt tggaatatca tgtaatttta aatccaaaaa
    46921 tacattatgc cctaacgatt tgatagaccg aacaatatct ggtccttgcg cataatataa
    46981 ttccatacca attttcacgt atagtttttc ttctgctgga aataagtcta aaaatgcttt
    47041 agtctcctca aatgatgaaa agtctaaagc aataattggg cgttcttctt tcataactta
    47101 tcctttctat aatgtgtcaa tatctcatta agcaccaaaa agcctcgcta ccaaagaagc
    47161 gaggctacag caaactaaaa gggaattccc tttttttatc ttgtatacct tgtttgcctc
    47221 tctggactca cttaaaggtg tctaaattta aactattata gcttttttaa aacaagatgt
    47281 caaaactttt tgttatttga acacattttt cctactatat ctttttttgt acacttacgt
    47341 caactcattt tatcattttt acccaatcat ttattcaaac tcacctgtgg gtaccttagg
    47401 gctttggtaa tattttataa aagcttcttc ttcaagatta accctctgga tatgttcata
    47461 gagcacatcc ggaataggag cttccttaag tgacaaagca ctcactttta aagatgaaaa
    47521 aacatcactt aagaccccaa aaactcgaat gcgtttatct ccaataataa tttgaaagac
    47581 ttctttggac tggcataaat ctatcagatg atccactcct ttaatccaat tatcgacaat
    47641 atccaaagtt ttgttcatca acttaaaagc tgattcttca taccaaaagg tagactcaat
    47701 caagcgttgc tgcatatcca aggtatcaat ggccgcaaca atatctccca actcagactg
    47761 tactaaatga gctggaaatt cccctccaag tatagaagga gttcgttcta caagcttcag
    47821 ttcatctcga aaaagagtca aatagtggat actgaccaat aacttttgga ttaagatttg
    47881 atgactattt tgttcagtaa cattcacacg accaccaccc tttgattaga aaaataccta
    47941 tttgttaata ctattaatat aacacaagaa aaataaaagg gcaatgaaat tgatagattc
    48001 aactctcaaa attgaatcat atttatccta ggccatatcg agaaaagact ccagcgcttt
    48061 ttcaaagttc taaacccatg atgacgtttg tgcttgcgtc tttatttttt ttgagctaga
    48121 ctgtacttat gagaattcaa caactgcatt atattatcaa aattgttgag tgcggctcta
    48181 tgaatgaagc tgctaagcaa ttatttatca cacaaccaag tctatccaat gcggtcaagg
    48241 atttggaaat ggagatgggc attaccattt tcaaccgaaa tcctaaaggg atcaccttga
    48301 caaaagatgg ggtggaattt ttatcttatg ctcgccaaat tatagagcaa acatcactgt
    48361 tagaagaccg ctataaaaac cacaatactg gccgagagct ctttagcgtt tcatctcaac
    48421 attatgcctt cgtagtcaat gcatttgtct cactcctcaa gcggaccgac atgactcggt
    48481 acgaactctt tttgcgtgag acaagaactt gggaaatcat tgatgatgtc aaaaactttc
    48541 gatctgaaat tggtgttctc tttatcaacg actataaccg agatgtcttg accaaattat
    48601 ttgatgacaa tcatttgact gcctctccat tatttaaggc acagcctcat atttttgtca
    48661 gcaaatcaaa tcctctagca actaaaagcc tattaaacat ggacgacttg agagattttc
    48721 cttacctgag ctatgaccaa gggattcaca actcctttta tttttcagaa gagatgatgt
    48781 cccagatgcc ccatagcaaa tcaattgtgg tcagtgaccg tgccaccctc tttaacttaa
    48841 tgattggttt agatggctac accgttgcca gtggtattct aaatagcaat ctaaatggtg
    48901 accagattgt ggctatcccg ctagatgtcc ctgacgaaat tgacattgtt tttatcaagc
    48961 atgaaaaagc caacttgtcc aaaatggggg aacgttttat cgaatacctt ctcgaagaag
    49021 taacctttga ttaatttctt tttcaatcgt cacactaaaa aacacggtag taagacctgt
    49081 atcggtcaag ttatcgtgtt ttttgataaa tatcagacaa tagattgtct ccaaaggccc
    49141 aactccgttt taataatggc attcatgtca tcaaagtaaa aaggttcacg tcggtagatt
    49201 acaccagaaa aagtcgccaa ggtgataatg ttttccaaaa aagcctgtga agaaaaatga
    49261 gtggtgcgat gcagttcctc ttcatcaaaa ggcttaatca aatgagccag aggattacgg
    49321 acagcctttt caaaatgtcg taacaaaaaa accaagtcct tgatttcctg aggtaaggag
    49381 gtcaacgtca acatgtctgc taagcttttg gtcgtcacat tgcgagactg cttttgagac
    49441 aggtaagcct taaaaacctc gtgatcagat tccaacatgg cctgaaaatg ccaggtatca
    49501 tattggtcat tctttgtatc aaaaatataa gccttgaagt ttggaatttc tgacgtgatt
    49561 agtctgagaa aaagtcggta aagaataggc gacactgacc tgacgaaatc aataatagca
    49621 ccgtttttta ctttaacttc taaatccaaa atgtaattgg caatctgctc atcttcaagc
    49681 gattggttaa gccataatga acagtggtac tggtcttcaa tgatccgatt ctctgctgca
    49741 tgttccgcca gaaaggcaag gttcaattct ctgcgctctt ttaacatctc tagtaaatag
    49801 agggcagttt tagggatatt tggcaaggct ttagcaattc taaaggcgtg attataagca
    49861 tagacatcca aaaggatgtc tagtgtttga ttattagtca ttggcaatta aggtttccaa
    49921 ggcgaccttc atcatatcag tgaaagtggt ttggcgctct tcagctgtag tatcttcggt
    49981 tggatcattc aagttatctg agatcgtcat gattccaagg gctttgacat ggtgctgagc
    50041 agctaaatag taaagagctg ctgcttccat ttcgatggct ttaacaccca atttaccaag
    50101 agccatatta cgctcaggca tgttagtgta aaagacatca gaagaaagca cattcccaac
    50161 gtgagttgtt actcccattt ctctggcgat atggtaagcc ttgtcaagta aaccaaaatc
    50221 agcaatttgt gggaaatcaa attctgggaa gtcattacgg ataatatttg agttggttgc
    50281 tgctgcttgg gcaagcacca attcacgcac atgaacttct gggtcaatcg cacccgcagt
    50341 accaacacgg atcaatgttt tgacaccgta atcaacgatt aattcacgag catagataga
    50401 gatcgaaggc atccccatac cagttcccat gacagaaacg cggcgccctt tgtaagttcc
    50461 tgtgtagcca aacatgttac gcacttcgtt aaaacacact gcatcttcta ggaaattttc
    50521 agcgataaat ttcgcgcgca aagggtctcc aggtagaaga attttatcag caatatcacc
    50581 ttttttggca gaaatatgga tagacattat taagatatta aaggcgacaa agaacaaagt
    50641 aaaaatagga aactgacgac gcatcccaga tgctagacag tttatctttt ttacacagtt
    50701 ctccgcccgt attcaattca atgaatacag ttagccctac ctttcctttt attttgtaga
    50761 aggtcagagg gcggacagaa agcaactgaa atttagggaa ctaacgccat gtgtgagcac
    50821 aaaagttagt ttatctagtt tccgagcttt ccgcccgtat tcaattgaat acaaccccat
    50881 tcctttcata attgatattg aatagaagaa gagagctaaa aatcgagttc aaataagaaa
    50941 ggactaactc tctttccttt attatttaaa aattaatacg aagaaatatt cgagtccatg
    51001 cttaatttaa accgaatacg tctctttccc ttcgttggtt atgttagtta gaaaagctag
    51061 aatctgagtt caacattcat ggaactcaca ccccgtgatt attttttgat ttttttattg
    51121 cgaacagatg tgccaaactg acgacataaa ataacattcc tgtaataatg ctccagttga
    51181 ttttgttcgt cctacaactc agctaaaata gctttaacaa gacctttgaa atcttcttta
    51241 atatgttcag tcacttcaac aacttcttcg tggttcaatt ctgattggaa accagctgca
    51301 aagttagtga tggctgaaat gccaagtact ttaagtccag aatgagcagc cacaataact
    51361 tctggcacag ttgacatgcc gacagcatct gctcctaata ctttgaaagc acggatttct
    51421 gctggtgtct cataagttgg accagttaag cccatataaa caccatcttc tagtttgatg
    51481 ttcatttttt cagccacttc atgtgcctta ttgcgataaa ccttagtata ggcatcagac
    51541 atatcaggga agcgtggacc aaactcgtcc aagttttcac cgattagtgg gttatttccg
    51601 gtcatattga tatggtctgt gattgccatc aaggtacctg ggccgtagcc gataccacca
    51661 gctgcatttg tgacaagaac accttcacat cccagagctt tcatgacacg aacagggaaa
    51721 gtcaccactt caagcgggtt tccttcgtaa aaatggaaac gaccttgtag ggcaagtact
    51781 ttacgaccag ctaaatcacc gtagactaat tttccagcat ggccaacaac tgttgatttg
    51841 ccccagtttg gaatatctgc ataatcaata acaacaggat tctctacttc ttcagcaagt
    51901 tcaccaagtc ctgaacccag aattaatcca aattctggag cttcaattcc tttagcaact
    51961 aaaaagtctt ttgtttcgtt aattttagtc atcaatgaca tctttttctc cttttacctc
    52021 aatagggttc acaatctcgc catcctcttt aatataagga ctaggcaggg gaacaggaag
    52081 aatatccagt aaggtctcag aaggacggca aagttttact cctttgcgag tcacaacaat
    52141 aggacgattg atcaaaattg gatccgtcaa catggcatta ataacgtctt tctcagctac
    52201 tgcttgattt gctaatccat gagcctcaaa ttcaggaaca ttggttcgca agagttctcg
    52261 cgcagagatt cccatcgatt gtaagagctc aattaaagtc tcccgattag gtggcgtctg
    52321 caaatactca ataatagtag gctcaatacc tgcatggcga atcatggcaa gaacattacg
    52381 tgaagtgcca cagtttggat tatgataaat cgtgatttct tccattatca actaattttc
    52441 ttattttaag tgaccaagga atgactcccc aatcatggct atatcaactc caaaattttc
    52501 tgcaacagtc gctgaaatat ctgcaaaatg cccttgtgga ataagaccat ttccagtaaa
    52561 ggatgcagag taggcaagca atggaatgta ttcacgagtg tggtcggtgc ctgcataggt
    52621 tgggtcatta ccatggtcag cagtgatgag aagcaaatcg tcttctttca tatttgcgat
    52681 gatttcaggt aggcgattgt caaattcatg caagcagtcg cgataaccct ctgggtcacg
    52741 acgatgacca aagttggcat caaaatcaac gaggttagta aaggagaatc ccttagtaaa
    52801 ctcaggtaat tgaagagtct taataagcgt gtcaatacca tggctgttcg acttattgtg
    52861 tcccatgtcg tttgtaatac ctgaaccatt aaagatgtca ttaatcttac caacggcata
    52921 agttgggaca cctgcgtcag ccagtttgtt caaaacggta tcttggaaag gagacactgc
    52981 atagtcatga cggttagccg tacgtgtaaa gtttcctggt tcgcctacat aaggacgcgc
    53041 aataatacgg cctaaaaggg cagggcgttc aagcgtgatg gagcgagcat attcacaaat
    53101 cttgtaaagc tcttccacag gaataatgtc ttcatgggct gcaatttgaa gcactgggtc
    53161 tgctgaggtg taaacaatca actcacctgt ttccatttga cgcgaaccaa aatcatcaat
    53221 aaccgcagtt cctgaatagg gtttattagc ttcacgaata attttgcgac ctgagaattc
    53281 ttcaatctta gttaaaatct cttctgggaa accattccag aaagtgtcaa aaggttctgt
    53341 aatgttcaaa cccataattt cccaatggcc tgtcatggta tcttttccaa gagatacttc
    53401 ttccaatttg gtcacgtaac cagtaggatt atcttctgtt ggaacagttt ttagtggtat
    53461 tggacgagaa atgtttccaa gtccaatttt tgccatattt ggaaccgata agcctgctgt
    53521 ttctgaaata tgaccgaggg tgtcagaatc tgtatcagct acaccagcat taaagaattt
    53581 gtcagcatct ggtgctgcac caataccgac agagtctaaa acaaccaagt gaatacgatt
    53641 aaattttgac ataagtctcc aatctagggg aggttacggt tgactcccca atacctataa
    53701 aatacattaa cactgttata tgctaagact agcttgcttt aggcacttct aaaacagtaa
    53761 cgccgtcttt actagcaaca atcaccttat caaccatacc attaaagaga ccgtgttcca
    53821 caacaccaac tgttccatct aacaggtgac caaaagcaac aggatcttta atacaaccca
    53881 aatcaagatc aataatatag ttttgcatgt ctgtcaccaa acgactatca cctttcatac
    53941 gaaaagacgg tttgtaacca gctttttcaa agacacggaa caaacgatca gcaccatatt
    54001 gaacgacttc gactggtaac ttaaaggcac ccagatgttc taccatttta gaagcatcaa
    54061 caacccagat gtattctttt gttggtgttg caacaatttt ttccattagt aaggctgcgc
    54121 cgccaccctt gattccattg aaatccttat ccacttcatc agcaccatct acggtgaggt
    54181 caatgctatc aatatcatct atcgatttta aaggaatgcc taagacttct gcttgcttgc
    54241 tagtcacact tgacgtagtc acaccaacca cttgcagtcc ttcttgcttt acgcgacggc
    54301 caatttcctc aacaaaatag taagccgttg acccagttcc aagcccaatg gtcatgccat
    54361 cagtcacata ttgcgcagcg gtaacaccgg caattttttt gagtgcttcc atgattcccc
    54421 cttataatag aattgcgtct tagcacttta ttataccatg aaagcgccaa cttgttaatg
    54481 aaaacatttg tttttctctc attgacagcc taagtgctat agaataggat gatcttttta
    54541 aaacttatga tttattctaa agtcatcata taagcactta cttttcgtgt ttatttagct
    54601 ttttgaaaat caagtgtttt tgtttgacct ttactgacct tttggttata ataatactat
    54661 aagatgagta aaaataatgt ctgaatcatt ttattagcaa agggtttcct atcatgtgaa
    54721 atcctaacaa cgattctgcg actgcttaca aacatcttat agtctatttt ttattgcgag
    54781 gtaactgata tgacacattt ttctggtaaa gatccttttg taaatatgga tgatattttc
    54841 aaccaattaa tggcaaacat gggtggttat cgtagcgaaa atccccgcta tctcgttaac
    54901 ggtcgcgaaa taactcccga agaattccaa cactatcgtc aaacaggaca actccctgta
    54961 gcgacaacca aagcaacaac ttctcaaatg ttaacaccta aagctgatag tgtattgact
    55021 caactcggaa ctaacttaac acaagaagct cgtcaagggc atcttgaccc cgtcattggt
    55081 cgtaacaaag aaattcaaga taccgctgaa atcttggcac gacgaactaa aaataatcct
    55141 gtccttgtcg gagatgctgg tgtcggtaaa actgcagtca ttgaaggttt agctcaagcc
    55201 attgttaatg gagatgtccc agctgctatt aaaaacaagg aaatcgtatc aattgatatt
    55261 tctagtcttg aagctggtac acagtatcgt ggttcctttg aagagaccat tcaaaacttg
    55321 attcaagaag taaaagaagc tgggaatatc atcttatttt ttgatgaaat tcatcaaata
    55381 gttggtgctg gtgccacaag cagtgattca ggctcaaagg gacttgcaga tattcttaaa
    55441 ccagccttgt cacgaggaga attaacactg attggtgcta ctactcaaga cgaataccgc
    55501 aatactattt taaaaaatgc tgcactcgct cgtcgcttta atgaagttaa agtgaatgca
    55561 ccatcagctg aagatacttt tcacatttta atgggtattc gtaatcttta tgaacaacac
    55621 caccatatta cccttcctga taatgttcta aaagctgctg ttgattattc tattcaatac
    55681 atcccacagc gtagcctgcc agataaagca attgacttac tggatatgac agctgctcat
    55741 ttagctgcgc aacacccggt cactgactta aaaacactcg aaactgaaat agctaaacaa
    55801 aaagagaggc aagaaaaagc tgtcgccaaa gaagactttg aaaaagcttt agcagcaaaa
    55861 acacggattg aaacattaca aaagcaaatt gagcaacata accaaagcca aaatgttact
    55921 gctacagtca atgatatcgc agagtctgta gagcgcttaa ctggtattcc cgtctctaac
    55981 atggggacta atgatttaga aaggctcaaa ggaattagca gccgcttaaa aagccatgtc
    56041 atcggacaag atgaggctgt tgcagcagta gcacgtgcta ttcgacgaaa tcgagcaggt
    56101 tttgatgatg gtaaccgtcc aataggttct ttcctttttg tgggtccaac tggtgttggt
    56161 aaaacagagt tagctaaaca attagcactt gatttatttg gttccaaaga tgctattatt
    56221 cgacttgata tgtccgaata taacgatcgc actgctgtgt caaaattgat tgggacaacg
    56281 gctggttatg ttggctacga tgataataat aataccctaa ctgagcgtgt tcgtcgtaac
    56341 ccttatgcta ttgtcttact agatgaaatt gaaaaagctg atcctcaaat cattacactt
    56401 cttctacaag tgttagatga tggacgattg acagatggac aaggaaatac tattaacttt
    56461 aaaaataccg ttattattgc tacatcaaat gccggttttg gccaacaaga tacggaaact
    56521 tctgggttta acattatgga tcgcattgca ccttatttca gaccagagtt tttgaaccgt
    56581 tttaatagta tcattaaatt taatcatctg caaaaagaaa atttagaaga aattgttgat
    56641 ttaatgctag ctgaagtaaa tcaaaccacc gcaaaaaaag gtattagttt aacgattgct
    56701 gatgacgcta aagctcattt gatagattta gggtacaatc acgctatggg agctcgtcca
    56761 ttacgacgta ttatcgaaca ggaaattcgt gaccgtataa ctgattatta tcttgaccat
    56821 ccagaagtca aaaaacttca ggcaatccta gaagaaggac aactagtgat taggcaaaaa
    56881 gatcagtaat aaacttcttc aattgaataa ttttcaaaac atgaaaaaat ccgtcttgtg
    56941 actacgttat acacttgacg gacttttttt tagcgctttt ttaaaaataa ttttttcatc
    57001 gcctaataac tcaagtttct ttccaaatct ggtgtaatca aaataaccat taggtgtgag
    57061 caatcctttt gaaaaagaag tatgtcccgc tgctacttct tctttgatat caatggtgtg
    57121 caaaatacga ttaacttttc ctttgagaac aacagcagat aaatgtcctg ttttttgatt
    57181 atatttgcct ccgatctgag aagctaattt ctcaaaagct tgatctaaaa tcatttctaa
    57241 ttcatggcta gctggcatac tattctcaat aatttggcgt tctttactac tcaagtcacc
    57301 aacaactaaa tgattttcct ttaatgtctt taataattca ttttggtgat aagcagctaa
    57361 acgtttgaca aaaatgtctc gttcaacttg aacactaaaa ctcaagtcaa cgttgttatt
    57421 tttaactgtt agtatcatct gagatgacgt taataaacta tcatcaataa gagggctctg
    57481 atgcaaacca gtaagacgtt tagcaaaatc atcgcctaac ttctgatcaa tgcttgttgt
    57541 tcgccagata ccttcaatag agctattacg atatcgaaca ccaaaaagta cagcaagcaa
    57601 gataagaaaa ataataaata caaaaagggc attgcattga tggcataaca atgactttcc
    57661 tttagagcgc ccttgggcta cttgtgtcat gactcatctc cttaatataa aatactagca
    57721 tgtcatcatt aaggcgtcaa gtaaaatcgg taaacctaac tatgattttt tatgttttcg
    57781 ataagaaaaa caccctctac tgagaatgtt ttagcataga taaatcaaca cctatcgcta
    57841 gacctgctct aaaatagcat cccaagaatc atcgatacca atacgttcta ccgaagaaaa
    57901 tactataaaa gtatcagagt tgtcaaaatt caaagctttc ttgaccacag actcatgttt
    57961 gttccatttg ccacgaggaa tcttatctgc tttggttgcc acaacaataa caggaatgtc
    58021 atagtacttc aaaaagtcat acatctgaat atcttctttt gaaggggcat gacgtaaatc
    58081 aaccaaactc acgactgcac gcagattgtc tctgctggtt aagtattctt cgatcatctt
    58141 tccccatttg gcccgttcac ttttagaaac cttggcatag ccataaccag ggacatccac
    58201 aaatctaagc ttgtcatcaa tattgaaaaa atttaatagt tgggtttttc ctggcttgct
    58261 tgaggttctt gccaaatttt tgcgccccaa tatggtatta ataaaacttg atttaccaac
    58321 atttgaacgt ccagcgaggg caatttctgg caaatcatct ttggggtaat gagatttatt
    58381 ggcagcactc aataaaattg aggcgttatg ggtatttaag acttgttctt cagccattat
    58441 ttctccttaa gctgtctcta aaacgggttt tgacttgcct tcgacagcag ccttggtgat
    58501 acgcactttt gtgacatctt cttggcttgg aatttcaaac ataatgtcta acatcgtttc
    58561 ttcaataata gaacgaagac cacgtgctcc tgtcttacgc tcaatggctt tattggcaat
    58621 ggcttctaag gcttccttat caaatgccaa ttcgacgcca tcgtaagaaa gaagggcctg
    58681 atattgcttg accagggcat ttcttggttc tgtcaaaatt tgaatcaaat ctgatgtgtt
    58741 tagttgttca agggctgcca caactggtaa gcggccaata aattctggaa taagcccaaa
    58801 cttttgaata tcttctgaaa taatttcttg catataagaa gcattgtcat caattttacg
    58861 gctgttttga ccaaaaccaa taactttttc acctaaacgt tgtttaacaa tctcctcgat
    58921 gccatcaaaa gccccgccaa caataaacaa gatatttttt gtatcaattt ggatcatttc
    58981 ttgattaggg tgcttccgcc caccttgtgg gggaacactt gctaccgtac cttcaataat
    59041 tttaagaagg gcttgctgta caccttcacc agatacatca cgtgtgatag atacgttctc
    59101 acctttttta gcaatcttat ctatttcatc aacataaata ataccacgct ctgctcgttc
    59161 gacattatag tcagcagcct gaatcagttt gagtaaaata ttttccacat cttctccaac
    59221 ataccctgct tcagtcaaag aagttgcatc tgcaatagca aatggtacat tgaggctttt
    59281 agccagagtt tgtgctaaga aggtttttcc tgaacctgtt ggtccaatca tcaaaatgtt
    59341 ggacttttgt aaatccacat catcgtcatc acgactttct gtaaacgaga cacgcttata
    59401 atgattgtac acagcaactg ataaagcacg tttagcacga tcttgaccaa ccacatattg
    59461 atttaaaaca tcaaggagct cttttggttt tggaacttct gtcaaatcgg caaggacttc
    59521 ttcagccaat tcttctttta taatttcttg tgataaagct acgcattcat tgcagatgaa
    59581 aacattattt cctgcgataa tttttttaac gtcatcctgg cttttaccac aaaatgaaca
    59641 gtatacttta atatcgttag ttctacttcc tgccatttct tataattctc ttactttctc
    59701 ttatctttta ctgaatttta atattagtca atgtgttgta ttggttttta gtgattcttg
    59761 cttgatagct aaatcttcac cagtgctgtc ttcgcaagtc acatctcaaa acaatgtacc
    59821 atagaaggcg tgaatactat taaccagatt agtaaacgca tttaataaga acagacttcc
    59881 taagccataa cgattctttt taaaagctgg gatggcacaa agaacacaaa tcacgcacaa
    59941 aaaagccgta aatactccaa aaatactacg ttgcatcgct tatcatctcc tcagactatt
    60001 tgtgttttaa agcagtaacg gtaaaatcat aaggattttt agcgtctcga gcatagaacg
    60061 tttggctttc ttgcgtgaaa tgactaaaat ctagatttgg tcggtaggta tctcctttaa
    60121 aacgatgatg gataactgtt tttatgatac gatcgaaata accattaaac gcttctaaaa
    60181 ccttattccc accaatgata taaagtgttt tatcctgcgc atggtaccac tccaagactt
    60241 tttcaatact agtcattgtc aacacctcat ctacttgata atctctattt cttgtcatga
    60301 caagggtttg acgttgagga agtcttttgc aattcattcc ttcaaaagtc acacggccca
    60361 ttaagactgc ttgatgtagc gttgtctttt taaaatgttc taactctttt ggtaaatacc
    60421 atggtaactt acccgcaaca ccaataagcc ctgcttcatc ctctgcccaa atagcaataa
    60481 tttcctttgt cattaattat ttccttcatt ttaaactcta tttattttac caaatttatg
    60541 aacaaaatgc tcacaaaaaa tctaaataaa gtttaaatag caaggtcaaa gtgcaactga
    60601 ggcttcactg gatcataatt ttgtaattca aaatcatctg gtttaatatc aaagaaatta
    60661 gtcccatctg gaacatttaa aacaagctta ggctgactag caactggctg tcgctttagt
    60721 aattcttgag cctgatcaaa ttggttgtca taaatatgta aattgttaac aaaataaaag
    60781 aacttaccta ttttccatcc aaaatgttta gcaatcatca tctgcaaagc aacgtattgc
    60841 atggcattga tgtgatgagc aactaatatg tcattagaac gttgcgtcaa actagcatct
    60901 aaataaagat cttcccctac acgtctaaca tcaaacatga tttgaaaggc acatggcaac
    60961 aacccttttg tctcctcaaa tgcttcatag tcccagagag aaatgacatt acgacgattc
    61021 caaggatttt ctgctaattg ctttagtatt tttgagatga tatcatgttt tttgactaca
    61081 gcaccataac gttgcccaat agtacgtgtc tggtcaactt cccattcatt ccaataatga
    61141 acattatatt tagcttctag cacatctaaa ctgttggact gatcttgata tatccaaaat
    61201 aattccttta tagcagattt aattggaatg ggtcgtaaag ttgtgatggg aaactctcct
    61261 ttagctaaat catattcagc aaacgcccca gtaatatact tagaatgagc tgttcgacca
    61321 tctttatatt taggacgtgc ttgctcactc aatgatcctt cgtttataat tttttgaata
    61381 ttagctttaa aaatctggtc agcttttgtc atctctaata acctttctgt aagcatactt
    61441 catactattc taactaattt taccaaaaaa taccagaata aataaacatt tgaaaaactt
    61501 catgtggtca
//
LOCUS       DATXQK010000007        60710 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.007, whole genome shotgun sequence.
ACCESSION   DATXQK010000007 DATXQK010000000
VERSION     DATXQK010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 60710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60710
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.007"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            141..1337
                     /gene="tuf"
                     /locus_tag="VC394_000434"
     CDS             141..1337
                     /gene="tuf"
                     /locus_tag="VC394_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEP5993929.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETATRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDTKFEDIIMELMDTVDSYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGTVRVNDEIEIVGIKEETKKAVVTGVEMFRKQLDEGLAG
                     DNVGILLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILSKDEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTINVELIHPIAVEQGTTFSIREGGRTVGSGI
                     VSEIEA"
     gene            1578..2336
                     /locus_tag="VC394_000435"
     CDS             1578..2336
                     /locus_tag="VC394_000435"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEP5993930.1"
                     /translation="MSRKPIIAGNWKMNKNPQEAKAFVEAVASKLPSTDLVDVAVAAP
                     AVDLVTTIEAAKDSVLKVAAQNCYFENTGAFTGETSPKVLAEMGADYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGLTPIVCCGESLETYEAGKAVEFVGAQVSAALAGLSAEQV
                     ASLVLAYEPIWAIGTGKSATQDDAQNMCKAVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVKDYMACPDVDGALVGGASLEADSFLALLDFLN"
     gene            complement(2435..3670)
                     /locus_tag="VC394_000436"
     CDS             complement(2435..3670)
                     /locus_tag="VC394_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEP5993931.1"
                     /translation="MALIEISQEQFDHYCHSLVHHSFIQTSEMASLMAKRGAKPQFLG
                     LEKDGELKVAAMVFSQKVAGGWRMELNAGPNTNHPEELEHFYTQLKDYAKQNDVIELI
                     LKPYDNYQSFDTDGIPISHPNTDLISLLTALGYKHDGLKTGYPEGEPVWHYVKKLEGI
                     DSSRLTHSFSKKGKALIKKANTFGIKLRQLKRDELHHFKEITEATSDRRDYLDKSLSY
                     YQDFYDSFGDSCEFMVATLNFEDYLNNLKQRQLQLATSINKVKGDLGKNPHSEKKQNR
                     LKELSSQFETFQVRISEALHFLEEYGTKDVFLAGSLFIYTEQEAVYLFSGSYPKFNKF
                     YSPALLQEHAMLKAIHKGIKQYNFLGITGKFDGSDGVLRFKQNFNGFILQKPGTFRCY
                     PFPIKYHFIRLAKKLLNRY"
     gene            complement(3657..4883)
                     /locus_tag="VC394_000437"
     CDS             complement(3657..4883)
                     /locus_tag="VC394_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase FemX"
                     /protein_id="HEP5993932.1"
                     /translation="MYTYKIGISPEEHDQFVLAQPQAGLLQSSKWRKVKDNWKHERIS
                     FYENGVQVAAAACLIRKLPLGFTMIYIPRGPIMDYANFELLDFVIKTLKTFGKSKRAL
                     FVKIDPSLVIKQTLEGKESKENDVTLSIIAFLKKLGIEWSGRTKELEDTIQPRIQANI
                     YAKDFDFDSLPKKAKQSIRTATNKGVNVTIGGSELLDDFSALMKKTENRKGIILRGKS
                     YYQKLLDIYAGQSYITMASLDLPEQKKLLIQQLDKALAEQARLTDKSKPSKVAENQKT
                     IARLQKDLTILSEQLAAGKTRIPLAATLTLIYGETSENLYAGMDDDYRNYQAPLLTWY
                     ETAKEAFKRGCRWHNLGGVENQQDGGLYHFKARLNPTIEEFAGEFNIPVGLVSSLAIL
                     TYNLRKKLRSTTNGTN"
     gene            complement(4883..5692)
                     /gene="yidA"
                     /locus_tag="VC394_000438"
     CDS             complement(4883..5692)
                     /gene="yidA"
                     /locus_tag="VC394_000438"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEP5993933.1"
                     /translation="MSIKLVAVDIDGTLLTDDRRITDDVFQAVQEAKAQGVHMVIATG
                     RPIAGVISLLEQLELNHKGNHVITFNGGLVQDAETGEEIVKELMTYDDYLETEFLSRK
                     LGVHMHAITKEGIYTANRNIGKYTVHESTLVNMPIFYRTPEEMTNKEIIKMMMIDEPD
                     LLDAAIKQIPQHFFDKYTIVKSTPFYLEFMPKTVSKGNAIKHLAKKLGLDMSQTMAIG
                     DAENDRAMLEVVANPVVMENGVPELKKIAKYITKSNNDSGVAHAIRKWVLN"
     gene            complement(6148..7449)
                     /locus_tag="VC394_000439"
     CDS             complement(6148..7449)
                     /locus_tag="VC394_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEP5993934.1"
                     /translation="MNEKVFRDPVHNYIHIDNPLIYDLINTKEFQRLRRIKQVPTTAF
                     TFHGAEHSRFSHCLGVYEIARRVTAIFEEKYADIWNKDESLVTMTAALLHDIGHGAYS
                     HTFEVLFHTDHEAFTQEIITNPETEINAILVRHALDFPDKVASVINHTYPNKQVVQLI
                     SSQIDCDRMDYLLRDSYFSAANYGQFDLMRILRVIRPVEDGIVFEHSGMHAVEDYIVS
                     RFQMYMQVYFHPASRAVELILQNLLKRAQHLYPEQQAYFQKTAPGLIPFFEKKANLAD
                     YIALDDGVMNTYFQVWMASEDHILSDLASRFINRKILKSVTFDQDSRGELERLRQLVE
                     SVGFDPDYYTGIHINFDLPYDIYRPELENPRTQIEMIQKDGSLAELSQLSPIVKALTG
                     TTYGDRRFYFPKEMLELDDLFAPSKETFMSYISNGHFHFSQ"
     gene            7531..7917
                     /locus_tag="VC394_000440"
     CDS             7531..7917
                     /locus_tag="VC394_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEP5993935.1"
                     /translation="MKLQLTNHIRFGDETEIIQEIHDCEWREKGGYQYLIYQNTDKEK
                     VVIKYNETELTMSRFSNPQSIMKFFAGKKVLIALPTPMGVQQFLTDTSHYHLDCSCQK
                     LDLHYHLLQAQTEMLFASYHLELSWD"
     gene            8147..10828
                     /locus_tag="VC394_000441"
     CDS             8147..10828
                     /locus_tag="VC394_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEP5993936.1"
                     /translation="MSKEQRHEAFYTQSEETVLAQLETSREGLTSAQAKERLAEYGRN
                     ELDEGEKRSLFMKFLDQFKDLMIIILIVAALLSVLTEGMEGLTDAIIILAVVILNAAF
                     GVYQEGQAEAAIEALKSMSSPLARIRRDGHVTEIDSKELVPGDIVLLEAGDVVPADLR
                     LLEANSLKIEEAALTGESVPVEKDLSTAVSEDAGIGDRVNMGYQNSNVTYGRGIGVIT
                     NTGMYTEVGHIAGMLANADETDTPLKQNLDNLSKILTYAILVIAAVTFAVGVFLRGQH
                     PLEGLMTSVALAVAAIPEGLPAIVTVVLSLGTQVLAKRNAIIRKLPAVETLGSTEIIA
                     SDKTGTLTMNQMTVEKVYTNGTLQSSSADIAFDNTTLRVMNFANDTKVDPSGKLIGDP
                     TETALVQFGLDHNFDVREAMVAEPRVAELPFDSDRKLMSTIHKQADGKYFIAVKGAPD
                     QLLKRVTQIEENGQIRPITDADKKTILDTNKSLAKQALRVLMMAYKYSDALPTLETEI
                     VEANLVFSGLVGMIDPERPEAAQAVKVAKEAGIRPIMITGDHQDTAKAIAKRLGIIEE
                     DGVDHVFTGAELNELSDEEFQKVFKQYSVYARVSPEHKVRIVKAWQNEGKVVAMTGDG
                     VNDAPSLKTADIGIGMGITGTEVSKGASDMVLADDNFATIIVAVEEGRKVFSNIQKTI
                     QYLLSANMAEVFTIFLATLFGWDVLQPVHLLWINLVTDTLPAIALGVEPAEPGVMKHK
                     PRGRKSSFFDGGVKEAILYQGAFQTILVLGVYGFALMFPEHTSYHDVHADALTMAYVT
                     LGLIQLVHAYNVKSVYQSIFTVGLFKNKLFNYSIPVAFVALMATVVVPGFNQFFHVTH
                     LTITQWLVVIIGSLLMVVLVELVKAVQRSLGQDEKAI"
     gene            complement(10912..11907)
                     /locus_tag="VC394_000442"
     CDS             complement(10912..11907)
                     /locus_tag="VC394_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEP5993937.1"
                     /translation="MQRKVTIKDIAELAKTSKTTVSFYLNGRFDKMSEETKNRIKSTI
                     EATNYKPSIAARSLNAKSTKLIGVVIGDITNSFSNQIVKGIESKAQEFGYQIIIGNSN
                     YDPSREDELIEKMLNLGVDGFIIQPTSNFRKYSRIIDIKKKKVVFFDSQLYEHRTNWV
                     KTNNYDAVYDTIQQCIDKGYEHFIMITGNPNLLSTRIERASGFIDVLEANHLTHQEMI
                     IDENQTSSEAIAQFLQGSLTKKSLVFVPNCWALPKVFTAMKSLKFNIPEIGLVGFDNI
                     EWTKFSSPTLTTIIQPAYEEGEQATKILIDDIEGHSQEAKQQIFDCQVNWQESTF"
     gene            complement(11971..13878)
                     /locus_tag="VC394_000443"
     CDS             complement(11971..13878)
                     /locus_tag="VC394_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEP5993938.1"
                     /translation="MSLAFTRFKETVNPNFCRNYLLDYQADSYADRKRIADLLLTNTF
                     LFEDNWDMEPCHIPYHLDPITWQEAVTDDPEWNFMLNRQTYLQTLILVYLVERDERYL
                     LTAKGFILNWIESAIPLDPKGLATRTLDTGIRCFAWVKCLIYLNLFDALTKQEERLIL
                     ASLEKQLHFLHANYLDKYSLSNWGILQTTAILLADAYFGSDLDIAAATAFARKELTQQ
                     IALQILEDGSQFEQSTMYHVEVLKALLELTALVPDYLPQLRPTLLAMSDYLLKMTGPD
                     HKQIPLGDSDFTDTRDILTLAATILEEPHLKAAAFPTLDIDSLLLLGEKGVHTFEQLP
                     VQTLPTFAHHFEHSGHITINQENYYLFFKNGPIGSSHTHSDQNSICLYYKGQPLFCDA
                     GRYTYKEEPLRYALKSASHHSTAFLEEQLPEQINSSWAYLSYPKSNYCHLRQNGHVYF
                     IEGSYQTQFSDRNNYQHDRQILILPPGMFLIIDTIQAQGNHCLVSQFILDNHLEVKTD
                     HLSDLRLISDCPFTIEETILSKKYNQYLTSHKFIKRKPFKDKGCTSTLLVPDDTKVTP
                     LTPLQTGKRNPIETALSWHLKGKQFDYSICVLQEDLIKGEKLVLLNGHKIRGKVVVIN
                     HITNEIIRLKH"
     gene            complement(13964..14785)
                     /locus_tag="VC394_000444"
     CDS             complement(13964..14785)
                     /locus_tag="VC394_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEP5993939.1"
                     /translation="MTGSNKLTKKDYLKTALRAFFLQNGFNYSNYQGIGYANVIYPAL
                     KKHFGSDKKGLYQALEDNCEFYNTNPHFLPFITSLHLVMLENDRPEEETRNIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASLASDGLVLGPIFFFLAMNIILTAIKIGSGLYGYK
                     VGTSFIDKLSEQMAIVSRMANIVGVTVIAGLAATSVKITVPITFAAGKVDAANTAQKF
                     VTIQGMLDKIAPALLPALFTLLMYYLIKNKKWTTYKLVILTVIIGVIGSWLGILA"
     gene            complement(14772..15554)
                     /locus_tag="VC394_000445"
     CDS             complement(14772..15554)
                     /locus_tag="VC394_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEP5993940.1"
                     /translation="MDINLLQALLIGLWTAFCFSGMLLGIYTNRCIILSFGVGMILGD
                     LPTALSMGAISELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVTPEAALALST
                     PIAVVIQFLQTFAYTAFAGAPETAKKQLQKGNIRGFKFAANGTIWAFAFMGLGLGLLG
                     ALSMDTLLHLVDYIPPVLLNGLTVAGKMLPAIGFAMILSVMAKKELIPFVLIGYVCAA
                     YLQIPTIGIAIIGIIFALNEFYNKPKQVDATTVQGGQQDDWI"
     gene            complement(15573..16061)
                     /locus_tag="VC394_000446"
     CDS             complement(15573..16061)
                     /locus_tag="VC394_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEP5993941.1"
                     /translation="MTQPNIIMTRVDERLIHGQGQLWVKFLNCNTVIVANDAVSEDKI
                     QQSLMKTVIPSSIAIRFFSIQKVIDIIHKASPAQSIFIVVKDLQDAKRLVEGGVPITE
                     INIGNIHKTDDKVAITQFISLGETDKSAIRCLAHDHHVVFNTKTTPAGNSASDVDILD
                     YI"
     gene            complement(16097..17296)
                     /locus_tag="VC394_000447"
     CDS             complement(16097..17296)
                     /locus_tag="VC394_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEP5993942.1"
                     /translation="MTRSLKTIALEPIKQPERFTKEDFLSQEDITQALDLALKQVRLN
                     MDYFKEDFPTPATKDNQYAIMDNTEWTNAFWTGCLWLAYEYGGDDAIKALAQANDLSF
                     LDRVTRDIELDHHDLGFLYTPSCMAEWKLLKTPESREAALKAADKLIQRYQDKGGFIQ
                     AWGVLGKKEDYRLIIDCLLNIQLLFFASQETGDNRYRDMAINHFYASANHVIRDDASA
                     YHTFYFDPETGDPIKGVTRQGYSDDSAWARGQAWGIYGIPLTYRFLKEPELIQLFKGM
                     THYFMNRLPKDQVSYWDLIFGDGSEQSRDSSATAIAVCGIHEMLKTLPDHDPDKKTYE
                     AAMHSMLRALIKDYANKNLNPGAPLLLHGVYSWHSGKGVDEGNIWGDYYYLEALLRFY
                     KDWNPYW"
     gene            complement(17296..17733)
                     /locus_tag="VC394_000448"
     CDS             complement(17296..17733)
                     /locus_tag="VC394_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEP5993943.1"
                     /translation="MIAIIVMGHGHFASGIVSSLELIAGPQEKVTAIDFTTEMTAADV
                     QDQLSRALIPEEETLVLCDLLGGTPFKVAATLMESLPNTTCNVLSGLNLAMLIEASFA
                     RQTAASFDDLVSGLITCSKEGIVDWKTLSQQEDGTTDDELGGI"
     gene            18068..18862
                     /locus_tag="VC394_000449"
     CDS             18068..18862
                     /locus_tag="VC394_000449"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEP5993944.1"
                     /translation="MENMFSLQGKIALITGASYGIGFEIAKAYAQAGATIVFNDIKQE
                     LVDKGVAAYRELGIEAHGYVCDVTDEAGIQQMVSQIEDEVGVIDILVNNAGIIRRTPM
                     LEMAAEDFRQVIDIDLNAPFIVSKAVLPSMIAKGHGKIINICSMMSELGRETVSAYAA
                     AKGGLKMLTKNIASEFGEANIQCNGIGPGYIATPQTAPLRERQADGSRHPFDQFIIAK
                     TPAARWGTTEDLAGPAVFLASDASNFVNGHILYVDGGILAYIGKQP"
     gene            18887..19528
                     /locus_tag="VC394_000450"
     CDS             18887..19528
                     /locus_tag="VC394_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEP5993945.1"
                     /translation="MKIALINENSQAAKNGIIYDALTSVTDKHGYQVFNYGMYGTEGE
                     SQLTYVQNGLLASILLTTKAADFVVTGCGTGVGAMLALNSFPGVTCGFASEPTEAYLF
                     SQINGGNALSIPFAKGFGWGAELNLTLIFERLFAEPMGGGYPKERAIPEQRNARILSD
                     LKKITYRDLLAIVKDIDQDFLKETISGAHFKEYFFANAEPSELVAYLKSVLEQ"
     gene            19557..20558
                     /locus_tag="VC394_000451"
     CDS             19557..20558
                     /locus_tag="VC394_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEP5993946.1"
                     /translation="MSKLLLVGEPLIRVSPNQFQPLTNACDAQLFFGGSEANIARTLG
                     GFGLEARLFTALPDNPVGHAFHQFLKQSGVDMSLTAWQGHRVGLYYLENGFGCRASQV
                     YYDRCGSSFSALDKDSLDLAAIFEGISHFHFSGISLALGKKTQDLIEILAREARKRQV
                     CVSFDLNFRSSMIAVADAKRLFSHFAQYADIIFGMEPLLLDSDDFDMFDRNKADTTMI
                     RERLAGLYQRYQLQAIYHTERSNDAQGSNRFKAYAYDGQFYESCEVTTPVLQRVGSGD
                     AFVAGLLYQLLEGNEKQRNLDFAVATASLKCTVAEDQLYHSVQQVEAVLHNQRDVQR"
     gene            20563..21198
                     /locus_tag="VC394_000452"
     CDS             20563..21198
                     /locus_tag="VC394_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEP5993947.1"
                     /translation="MGKIEILTKLKANRLVLVVRGESSEEALACSLASIEGGIKTIEV
                     TYTNPFASEVIGQLAERFKEDPEVLIGAGTVLDDVTARQAILAGAQFIVGPNFNRAVA
                     LICHGYSIPYLPGCMTVNEVVTALESGVDMVKIFPGSTVGISFIRAIKSPLPQVEVMV
                     TGGVSSDNLKDWLAAGVDVLGIGGEFNQLASQKQYNLITKKAAHYIKSLRQ"
     gene            21494..22144
                     /locus_tag="VC394_000453"
     CDS             21494..22144
                     /locus_tag="VC394_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEP5993948.1"
                     /translation="MIKGIIFDMDGVLFDTEPFYLRRREDFFKTKGIPIDHLNSKDFI
                     GGNLQELWKELLGKNRDDAIVKAITTDYDAYKQAHKPPYQKLLITEVNSCLEQLKKQG
                     IKLAVASNSKRQDVLLALETTQIKDYFEIILAREDVSRGKPYPDIYNKAVQKLGLQKK
                     QLLVVEDSEKGIAAAKAANLTVFAITDYRYGIDQSQADHKIDHLGQLCVKIGCFGS"
     gene            22840..23964
                     /gene="queG"
                     /locus_tag="VC394_000454"
     CDS             22840..23964
                     /gene="queG"
                     /locus_tag="VC394_000454"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEP5993949.1"
                     /translation="MTIKAEIKALAKEIGISKIGFTTADNFDYLEKSLRASVEEGRNS
                     GFEHKVMEDRIYPERLLESAKTIISIGVAYPHKLPQQPQKTPYKRGKITPSSWGLDYH
                     YVVGEKLDRLSKGIEELCRDFPLQQKAMVDTGALVDTAVAQRAGIGFIGKNGLVISKE
                     YGSYMFLGELITNLEIEPDKPVDYDCGDCRRCLDACPTSCLIGDGSMNAKRCLSFQTQ
                     DKGMMDIEFRKKIKTVIYGCDICQICCPYNKGINNSPATEIDPELAQPELIPFLSLSN
                     GQFKEKFGMIAGGWRGKNILQRNAIIALANAHDKTAVVKLIEIIDKNNNPIHTATAIW
                     ALGEIVKKPNDEMLAFMSHLTLKDEDSQKELELIRRKWQV"
     gene            24033..25131
                     /gene="prfB"
                     /locus_tag="VC394_000455"
     CDS             join(24033..24104,24106..25131)
                     /gene="prfB"
                     /locus_tag="VC394_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889043.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEP5993950.1"
                     /translation="MEVAEIRQKIVENKEKLTSFRRSLDLDRLEEEIALLENHMTEPD
                     FWNDNIAAQKTSQELNELKGKYDTFHNMQELSDETELLLEMLDEDDSLKEELEENLMQ
                     LDKIMGAYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDLLLRMYTRFGNANGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFASVE
                     VMPELDDTIEVEVRDDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVSSTVDRT
                     QYGNRDRAMKMLQAKLYQLEQEKKAQEVDALKGDKKEITWGSQIRSYVFTPYTMVKDH
                     RTNFELAQVDKVMDGEINGFIDAYLKWRIED"
     gene            25150..25842
                     /gene="ftsE"
                     /locus_tag="VC394_000456"
     CDS             25150..25842
                     /gene="ftsE"
                     /locus_tag="VC394_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEP5993951.1"
                     /translation="MALIEMSGVTKKYRRSTTALRDVNVSVNQGEFVYLVGPSGAGKS
                     TFIKLLYREEQLTTGKLYVGEFNLTKLKARDVPILRRHIGVVFQDYKLLPRKTVFENV
                     AYAMEVIGEKRRHIKKRVPEVLDLVGLKHKMRSFPSQLSGGEQQRVAIARAIVNNPKL
                     LIADEPTGNLDPEISWEIMQLLERINVQGTTILMATHNSHIVNTFRHRVVAIEDGRIV
                     RDEEKGDYGYDD"
     gene            25835..26764
                     /locus_tag="VC394_000457"
     CDS             25835..26764
                     /locus_tag="VC394_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP5993952.1"
                     /translation="MIRYFFRHIWESIKNLKRNFWMTFASVSMVAVTLTLVGVFAATL
                     LNIQRVASGVENNVHINTYLQVDSTDAAKVIQNTAGEPVNNDNYHSVYDKIAQIKGVK
                     KITFSSKDEQLKKLQETLGDVWNMYDQDTNPLQDIYLIETQTPKQVKAITKKIRTIEG
                     VEAADYGGINSDKLFKFSTLIQTWGLIGTAMLLFVAVFLISNTIRMTIMSRKRDIEIM
                     RLVGAKNSYIRGPFFFEGAWVGLLGAVLPSLLIYYGYDLVYKHFAQELQRNNLSMYPL
                     DPYVYYLIGALFVIGIMIGSLGSVLSMRRYLKF"
     gene            complement(27074..27772)
                     /locus_tag="VC394_000458"
     CDS             complement(27074..27772)
                     /locus_tag="VC394_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEP5993953.1"
                     /translation="MPFIFRHSFFNKALIFWYTILMKIYKTINHIAGENTYYLVNDQA
                     VILIDPGSNGQEIIAKIKSFEKPLVAILLTHTHYDHIFSLDLVRDAFDHPPVYVSEKE
                     AAWLSSPDDNLSGLGRHDDIITVIARPAENFFKLKQPYQLNGFEFTVLPTPGHSWGGV
                     SFVFHSDELVVTGDALFRETIGRTDLPTSNFEDLITGIRQELFTLPNHYRVYPGHGPS
                     TTICHEKNANPFFH"
     gene            27940..28704
                     /locus_tag="VC394_000459"
     CDS             27940..28704
                     /locus_tag="VC394_000459"
                     /EC_number="1.1.1.304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-acetoin forming diacetyl reductase"
                     /protein_id="HEP5993954.1"
                     /translation="MSKVAIVTGAGQGIGFAIAKRLHADGFKIGILDYNEETAQAAVD
                     KLSPEDTVAVVADVSKRDQVFDAFQKVVDTFGDLNVVVNNAGVAPTTPLDTITEEQFE
                     KAFAINVGGTIWGSQAAQKHFRELGHGGKIINATSQAGCEGNPNLTVYGGTKFAVRGI
                     TQTLAKDLASEGITVNAYAPGIVKTPMMFDIAHEVGKNAGKDDEWGMEQFAKDITLKR
                     LSEPEDVANAVGFLVGDDSNYITGQTIVVDGGMVFH"
     gene            28812..31313
                     /locus_tag="VC394_000460"
     CDS             28812..31313
                     /locus_tag="VC394_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEP5993955.1"
                     /translation="MFCFIDIACYNRLTMTQKKLRKYAVVDLEATGAGPNASIIQVGI
                     VIIQGNKIIDSYETDVNPHESLDEHIVHLTGITDKQLAKAPDFGQVAHHIYQLIEDCI
                     FVAHNVKFDANLLAEALFLEGYELTIPRVDTVELAQLFFPRFEKYNLSHLSRQLNIDL
                     AEAHTAIADARATAILFLRLLQKIESLPIECLESLLAYSDSLLFETAMVIQEGLAKAK
                     PYDPNKYIKIRQILLPKGSKALKPYQISKSFPINMALLGLEERPKQTQFAQLIDEDYH
                     QGVASFIEAQTGIGKTYGYLLPLLAKEDQNQIIVSVPTKLLQDQLMAGEVAAIQEQFH
                     IACHSLKGPANYLKLDSFADSLDQNDQNRLVNRYKMQLLVWLLETKTGDLDEIKQKQR
                     FAAYFEQLKHDGDIKQSSEFYDYDFWRVSYEKAKTARLLITNHAYFLHRVQDDKDFTR
                     NKVLVFDEAQKLMLQLDQLSRHQLNLTVFLQTIQAKLSNPLPLLEKRLLESLSFELGQ
                     VSSDYYQNKEHQLAHDWSRIAGYAKELTGADYQELQAFFATSDGDYWLSSEKQEEKRV
                     TYLNSASKAFIHFQQLLPETVKTYFVSATLTISSEVTLADLLGFEEYLYHVIEKDKKQ
                     DQLVLVDQEAPIVTEVSDQIYVEAIAKRIESLKQEGYPILVLFNSKKHLLLVSDYLDQ
                     WQVPHLAQEKNGTAYNIKKRFDQGEQTILLGLGSFWEGVDFIQADRMITLIARLPFDN
                     PEDFFVKKMSHYLLEKGKNPFRDYFLPMTILRLKQAIGRTMRRQDQKSVVIILDRRLL
                     TKSYGQVILEGLGQEFLISQQNFHDCLVETDCFLI"
     gene            31648..32841
                     /locus_tag="VC394_000461"
     CDS             31648..32841
                     /locus_tag="VC394_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEP5993956.1"
                     /translation="MPKLSKRVLEMKESVTLAAGARAKALKAQGRDVLNLTLGEPDFF
                     TPKHIQDKAIESIQNGTASFYTNASGLPELKAAIATYLKNQYGYHLSPDQIVAGTGAK
                     FILYAFFMAVLNPGDQVLIPTPYWVSYSDQVKMAEGQPIFVQGLEENQFKVTVDQLER
                     ARTSKTKVVLINSPSNPTGMIYGAEELRAIGEWAVHNDILILADDIYGSLVYNGNQFV
                     PISTLSEAIRRQTITVNGVAKSYAMTGWRVGFAAGEPEIISAMSKIIGQTTSNLTTVS
                     QYAAIEAFCGSQSSLEEMRLAFEERLNITYPLLCQVPGFEVVKPQGAFYFFPNVKKAM
                     EMTGFSDVTSFANAILEEVGLAVVSGAGFGAPENVRLSYATDIETLKEAVRRLHVFME
                     SNEIN"
     gene            32871..34217
                     /gene="asnS"
                     /locus_tag="VC394_000462"
     CDS             32871..34217
                     /gene="asnS"
                     /locus_tag="VC394_000462"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEP5993957.1"
                     /translation="MSKKLISIVDVKDYVGQEVTIGAWVANKSGKGKIAFVQLRDGSA
                     FFQGVAFKPNFIEKYGEESGLEKFDVIKRLNQETSVYVTGIVKEDERSKFGYELDITD
                     LEIIGESHEYPITPKEHGTDFLMDNRHLWLRSRKQMAVMQIRNAIIYATYEFFDQNGF
                     IKFDSPILSENAAEDSTELFETDYFGKPAFLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSFLSHEESLDLQEAYVKALIQGVLDRAPQALDILERDVE
                     ALKRYITEPFKRVSYDDAITLLQEHEADEDTDYEHLEHGDDFGSPHETWISNYFGVPT
                     FVVNYPASFKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREDNYDALVAKMDEL
                     GMDKSEYDFYLDLRKYGSVPHGGFGIGIERMVTFVAGTKHIREAIPFPRMLHRIRP"
     gene            34632..35522
                     /gene="rapZ"
                     /locus_tag="VC394_000463"
     CDS             34632..35522
                     /gene="rapZ"
                     /locus_tag="VC394_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002282100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEP5993958.1"
                     /translation="MSDKHINLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALVPK
                     FLELIEQTNENRRVALVVDMRSRLFFKEINSTLDSIESNPSIDFRILFLDATDGELVS
                     RYKETRRSHPLAADGRVLDGIRLERELLSPLKSMSQHVVDTTKLTPRQLRKTISDQFS
                     EGSNQASFRIEVMSFGFKYGLPLDADLVFDVRFLPNPYYKVELREKTGLDEDVFNYVM
                     SHPESEVFYKHLLNLIVPILPAYQKEGKSVLTVAIGCTGGQHRSVAFAHCLAESLATD
                     WSVNESHRDQNRRKETVNRS"
     gene            35519..36496
                     /locus_tag="VC394_000464"
     CDS             35519..36496
                     /locus_tag="VC394_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEP5993959.1"
                     /translation="MKNPKMTVIGGGTGISIILKSLRNEAVDITAVVTVADDGGSSGE
                     LRNAMQLTPPGDLRNVLLAMSDMPKFYERVFQYRFNESDGALAGHPLGNLIIAGISEM
                     QGSTYNAIQILTKFFHITGKIYPSSEQALTLHAVFKDGHEVAGESSIAKYQGMIDHVY
                     VTNTYNDQKPQASRKVVEAILESDMIVLGPGSLFTSILPNLVIPEIKEALRQTKAEVV
                     YICNIMTQYGETEQFSDADHVAVLNQHLGRDLIDTVLVNVAKVPQAYMNSNKFDEYLV
                     QVDHDFAGLCRAAKRVISSYFLRLENGGAFHDGNLVVEELMNLVRIVKQ"
     gene            36493..37404
                     /gene="whiA"
                     /locus_tag="VC394_000465"
     CDS             36493..37404
                     /gene="whiA"
                     /locus_tag="VC394_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEP5993960.1"
                     /translation="MSFTTKVKEELIHLSTGDNNELAAIIKLSGSLGLAHQSLHLSIT
                     TENAKIARYIYSLIEDAYVIVPEIRYHQKTNLRKNRVYTVYVEQGVETILADLKLADS
                     FFGLETGIEPQVLSDDNAGRSYLKGAFLAAGSIRDPESGKYQLEIYSVYLDHAQDLAQ
                     LMQKFMLDAKTIEHKSGAVTYVQKAEDIMDFLIIIGAMSCKEDFEAIKLLREARNDIN
                     RANNAETANIAKTISASMKTINNIIKIMDTIGLESLPIELQQVAQLRVKHPDYSIQQV
                     ADALEFPITKSGVNHRLRKINKIADDL"
     gene            37537..38934
                     /locus_tag="VC394_000466"
     CDS             37537..38934
                     /locus_tag="VC394_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HEP5993961.1"
                     /translation="MACTTILVGKKASYDGSTMVARTEDSQNGDFTPKKMIVVKPEDQ
                     PRHYRSVQSSFEMDLPDNPMTYTSVPDALGKDGIWAEAGVNEANVAMSATETITTNSR
                     VLGADPLVASGIGEEDMVTLVLPYIRSAREGVLRLGAILEDYGTYESNGVAFSDEHDI
                     WWLETIGGHHWIARRVPDDAYVTNPNQFGIDYFEFNNPEDYLCSADLKDFIDTYHLDL
                     TYNHEHFNPRYAFGSQRDKDRHYNTPRAWIMQKFLNPEIVQDPRSFALAWCQKPYRKI
                     TVEDVKYVLSSHYQDTVYDPYGSEGTPVSKKVFRPIGINRTSQTAILHIRPNKPQEIA
                     AIQWMAYGSMPFNTMVPFFTQVKTIPDYFANTYENVSTDNFYWTNRLIAALADPHYNH
                     HETDLDNYLEETMAKGHAMLHAVEAQLLAGETVDLEEENQKMSDYVQGETQTLLNKIL
                     FDASNLMTNRFSLSD"
     gene            39086..40633
                     /locus_tag="VC394_000467"
     CDS             39086..40633
                     /locus_tag="VC394_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEP5993962.1"
                     /translation="MKKKILLMMSLISVFFAWQLTQAKQVLAEGKVKVVTTFYPVYEF
                     TKGVIGNDGDVSMLMKAGTEPHDFEPSTKDIKKIQDADVFVYMDDNMETWVSDVKKSL
                     TSKKVTIVKGTGNMLLVAGVGHDHHHEEADKKHEHNKHSEEGHNHAFDPHVWLSPYRS
                     ITVVENIRDSLSKAYPEKAENFKANAATYIEKLKELDKDYTAALSDAKQKSFVTQHAA
                     FGYMALDYGLNQISINGVTPDAEPSAKRIATLSKYVKKYGIKYIYFEENASSKVAKTL
                     AKEAGVKAAVLSPLEGLTEKEMKAGQDYFTVMRKNLETLRLTTDVAGKEILPEKDTTK
                     TVYNGYFKDKEVKDRQLSDWSGSWQSVYPYLQDGTLDQVWDYKAKKSKGKMTAAEYKD
                     YYTTGYKTDVEQIKINGKKKTMTFVRNGEKKTFTYTYAGKEILTYPKGNRGVRFMFEA
                     KEPNAGEFKYVQFSDHAIAPEKAEHFHLYWGGDSQEKLHKELEHWPTYYGSDLSGREI
                     AQEINAH"
     gene            40781..41503
                     /locus_tag="VC394_000468"
     CDS             40781..41503
                     /locus_tag="VC394_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEP5993963.1"
                     /translation="MPKEQPLYLQIVDDLEVKIKKSMTENDKLLSERELSDLYGVSRI
                     TIRLALKELELRDLIYKKQGKGTYVSGIKEPVTDLSSTYSFTEQMKKVGKTPKTEILS
                     FEQYQVTPYLSGLLEFDLDTEVFELERLRIADDMPLMFERSYIPAQPFQGLSIADLKR
                     KALYDIFAKDYQETIRLAEEEFYASIALDYEADLLGIKKGDPVLHIIRKTYNDKNILI
                     EFTFSIARADQFRYRVQHHPNG"
     gene            41523..42722
                     /locus_tag="VC394_000469"
     CDS             41523..42722
                     /locus_tag="VC394_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEP5993964.1"
                     /translation="MFTKTQEALEALGAAITTKEIKQEPRLWQETMTFFEETRDSLDS
                     FLKRVCKSANGNNVHVIFTGAGTSEYIGNTICPYLKKVGNRQCYLFESVASTDLVAAP
                     DYYLAEEETVLLVSFARSGNSPESVAAVNLVNQLVPNSYHLTITCAKDGELAKKAQQD
                     ERSYLYLMPEDANDAGFAMTGSFTCMMLAALLIFDDANSIAQKEAYVTDMILAGQAVI
                     DQERRLQELADLGFERLVYLGSAGLAKLTQEAQLKMLELTAGQVATLYESSMGFRHGP
                     KSFINDRTLVIGFVNNDAYVRQYDLDMLEEIQADGIALKILALLQGGDINFSEDQFRL
                     DTRHLLPEAYLAFPMILVAQTLALLTAVTLGNSPDTPSATGTVNRVVKGVTIHPYPTS
                     SRQCNKA"
     gene            complement(42819..43079)
                     /locus_tag="VC394_000470"
     CDS             complement(42819..43079)
                     /locus_tag="VC394_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEP5993965.1"
                     /translation="MRKDIHPDYRPVVFLDTTTGYQFLSGSTKASKETVEFEGETYPL
                     IRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAK"
     gene            complement(43194..44135)
                     /locus_tag="VC394_000471"
     CDS             complement(43194..44135)
                     /locus_tag="VC394_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEP5993966.1"
                     /translation="MITTFETILDKIKAHQTIIIHRHQNPDPDALGSQAGLKEIIAQN
                     FPDKKVLMTGFDEPSLTWISQMDQVTDKDYKEALVIITDTANRPRIDDERYTLGQCLI
                     KIDHHPNDDVYGDFYYVDTSASSASEIIADFAFSQNLTLSDKAAKLLYTGIVGDTGRF
                     LYASTTSKTLSIASQLRHFEFDFAAISRQMDSFPLKIAKLQSYVFEHLTIDESGAAYV
                     LVSQETLKHFDVTLAESSAIVCAPGKIDNVQAWAIFVELTDGNYRVRMRSKEKIINGI
                     AKRHGGGGHPLASGANSANLEENQAIFRELIAVCQEI"
     gene            44529..44978
                     /locus_tag="VC394_000472"
     CDS             44529..44978
                     /locus_tag="VC394_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEP5993967.1"
                     /translation="MALAKIVYASMTGNTEEIADIVANKLQELGHDVDIDECTTVDAS
                     EFENADIAVVATYTYGDGDLPDEIVDFYEDLQDLDLEGKIYGVVGSGDTFYDYFCKSV
                     DDFSDQFALTGAVKGAEPVKVDLAAEDEDIDRLEAFAEQLSQAVNNL"
     gene            45153..45437
                     /locus_tag="VC394_000473"
     CDS             45153..45437
                     /locus_tag="VC394_000473"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEP5993968.1"
                     /translation="MRLEEIRQEINGIDHHLVALLEKRMALVEQVTAYKLANHLPVLD
                     QARENQILDRVSYLVKDQAFEPAIHETFKTIMSLSRQYQTQRLTGGDIND"
     gene            45430..46692
                     /locus_tag="VC394_000474"
     CDS             45430..46692
                     /locus_tag="VC394_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HEP5993969.1"
                     /translation="MIKSIATLPKPSLKEALGLIGLALPIGATVGVVDVIFGKGLLFL
                     SEYRDHHLFLLLPFLALAGLVIVFLYDKLGKEVRQGMGLVFQVGHGQKDQIPPILIPL
                     ILFSTWVTHLFGASAGREGVAVQIGATISHYCQRFVTSQEAARHLLIMGMAAGFAGLF
                     QTPIAAVVFALEVLLVGTLRYCALLPSLVAACVASWTSHSLGLEKFTIVLEETLTITP
                     LTLVKLIGLGLIFGLVGNSFAYLLGWFKPYLSQKLPNPYFRIAFIGALLSICLMIGHV
                     GRYSGLGTHLIAAAFSGQTILTYDWLLKMIVTVISLSAGFQGGEVTPLFAIGASLGIV
                     LAPYLGLPVLLVAALGYTTVFGSATNTFWAPIFIGIEVFGPENALAYFVTSAAAYMVS
                     HRHSIYSYQKVVMINLKDMTVNQMDLKK"
     gene            46807..47154
                     /gene="rplS"
                     /locus_tag="VC394_000475"
     CDS             46807..47154
                     /gene="rplS"
                     /locus_tag="VC394_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEP5993970.1"
                     /translation="MNPLIQSLTEGQLRSDIPNFRPGDTVRVHAKVVEGTRERIQIFE
                     GVVISRKGQGISEMYTVRKISGGIGVERTFPIHTPRVDKIEVIRHGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            47401..47472
                     /locus_tag="VC394_000476"
     tRNA            47401..47472
                     /locus_tag="VC394_000476"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:47434..47436,aa:Arg,seq:cct)
     gene            complement(47517..48593)
                     /locus_tag="VC394_000477"
     CDS             complement(47517..48593)
                     /locus_tag="VC394_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006740075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEP5993971.1"
                     /translation="MWIEELLNGKFKFIERYTDPLTGKYKKVSVTLDKNSNQAKNKAS
                     IIIKEKIEDRLAIRDHSEMTYGELKREYLNQWIPTVKDSTKRGYLVSDGHIATVLPDD
                     TIISKLTKRDIRQFIDNLLKNHSYHVTHKCRKRLHAILSYAIQMDYLTSNPTDNVLVP
                     KPKEEYMPEKVLYLTSNEVYDLCGRMIANNEQTLADVVLFMFLTGMRYGELACLTYDK
                     IDFENKEILINATYDFYTKEITTTKTKKSTRKISVSDNILEIINRQNKVSEFIFPNSK
                     GLPILNAYINKRLKIYGNYHTHLFRHSHISFLAEKGIPLKAIMDRVGHSDPNTTLSIY
                     SHTTVNMKALINKQTAPFMPLKII"
     gene            complement(48712..49233)
                     /locus_tag="VC394_000478"
     CDS             complement(48712..49233)
                     /locus_tag="VC394_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5993972.1"
                     /translation="MKKIVYLFTLSVLIVCLGACSGGSKKDTTSKTLSSDTSKVSGDM
                     SEKEYFDTLISKIDKVTTDNYKSEEYLFYDYKTILRDPKKYFSLKVRIDNLKIIQISD
                     KDKYTKILANTPNGDLYMLFIETKRLETKLLETDNITINGRYLLSYEYTTTSGSENSV
                     PLIYIDGYLLLDK"
     gene            complement(49244..49621)
                     /locus_tag="VC394_000479"
     CDS             complement(49244..49621)
                     /locus_tag="VC394_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEP5993973.1"
                     /translation="MVKINEILSQYNIKLFEFPEAMWDRKGFYYPDKRIIYINQNLSQ
                     IEKEKVILHELGHLEHDPKQYQRLLLKYENQADRFMIRELIKNYLSSHDVVDFNWLQF
                     ATTYQISTTWGQEIIQDEFKKLI"
     gene            complement(49605..49964)
                     /locus_tag="VC394_000480"
     CDS             complement(49605..49964)
                     /locus_tag="VC394_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP5993974.1"
                     /translation="MRTNDEIIDLMDNIRKEKNLSISELSRKVGMSKSGVSLYFNKAR
                     KFPLDRANLFAKALGTTPEHIIGVTPKNSPTQTIDLSNLRERVVMFDGKPLSDTDVEK
                     IEAIIKLSLGVGNGEDK"
     gene            50152..50370
                     /locus_tag="VC394_000481"
     CDS             50152..50370
                     /locus_tag="VC394_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP5993975.1"
                     /translation="MAVDYLRVKAERIAKGYTQDYMAKQLGWSDRARYAKRENGFVSF
                     DADELAKVAEILGISKDDIGIFFTYNVH"
     gene            50470..50700
                     /locus_tag="VC394_000482"
     CDS             50470..50700
                     /locus_tag="VC394_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5993976.1"
                     /translation="MLAKLKSGIEVPYEELWMDDNDLAEFIGKSFDQTQRLLRKMYKD
                     RNYRKYIDKVGGRSTKVKKFEEWRKTQNEKLI"
     gene            50684..50827
                     /locus_tag="VC394_000483"
     CDS             50684..50827
                     /locus_tag="VC394_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5993977.1"
                     /translation="MKSLFNFIFAKPKKQEEKPKWTIENNGWEANARRYNQLHGLPGK
                     QIW"
     gene            50837..51055
                     /locus_tag="VC394_000484"
     CDS             50837..51055
                     /locus_tag="VC394_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5993978.1"
                     /translation="MEQQLINDNEFLRDENRRLNNELAEHYFVVTAKANLLDVIIASG
                     FILQSTIDKCIAELDEIDQMELRKVWNK"
     gene            51057..51299
                     /locus_tag="VC394_000485"
     CDS             51057..51299
                     /locus_tag="VC394_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5993979.1"
                     /translation="MRLAKQKIGAQQNGYGRIGCDIEIYNEIAEIADKCGYTLTSLTN
                     ALLGYALENSELTAETKTVEVFKFTIGNEDFDNGNN"
     gene            51286..52602
                     /locus_tag="VC394_000486"
     CDS             51286..52602
                     /locus_tag="VC394_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEP5993980.1"
                     /translation="MVTINKLEIENVKRIKAVKIEPSATGLTIVGGNNNQGKTSVLDA
                     IAWALGGNKYKPSQATREGSMVPPTLKITMSNGLIVERKGKNASLKVIDPNGQKGGQQ
                     LLDSFVEELAINLPKFMDGTPKEKADVLLEIIGVGDQLAELEFKEKEIYNQRHAIGVI
                     ADQKEKFAKEMTYYPDAPKQLVSISELIQQQQAILAKNGENAQKRQNVERIRYDYDQS
                     ILEVDRLRKLLADAEAKTNKLSEDLKIANTDAMDLHDESTAEIEANIADIDEVNRKVR
                     ANFDKDKAEEDAKQQRDQYNTLTNEIEVIRQQKRDLLTNADLPLEGLTVDDGKLLYLG
                     QEWDNMSGSQQLMVATAIVRKLKPECDFVLIDKLEQMDNITLTQFGQWLEQEGLQAIA
                     TRVSTGEECAIIIEDGYSVENKAHNFETAAAGGFAETVAPAWQGGF"
     gene            52617..53699
                     /locus_tag="VC394_000487"
     CDS             52617..53699
                     /locus_tag="VC394_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEP5993981.1"
                     /translation="MQITRGKRARAQRVVIYGPEGIGKSSFAAQFPEPLFIDTEGSTD
                     NMDVARMDKPTSYTMLKNQIAWIKANPTCCKTLVLDTIDWAESLIVDDVCAQHGKKGI
                     EDFGWGNGYTYTKEEVGRFLNSLQELIEFGINVVLTAHAQMRKFEQPDEMGAYDRWEL
                     KLGKKTSSQTAPLVKEWADMVLFANYKTVVMTTDTNKKKATGGSRVLYTQHHPAWDAK
                     NRHGLPEEMPLDYAGIAHIFAQQAPSQPVAETPSTQQPTPTQSAHEPAHKQPTQPPVA
                     NESQAPLPDTLPQAMKDLMVPDQVTADELVQVANIRGHFPLGTPIENFPPNYWDMIVA
                     NWSATLEVIKTQVRVEPDLPFTVEGT"
     gene            53894..54487
                     /locus_tag="VC394_000488"
     CDS             53894..54487
                     /locus_tag="VC394_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5993982.1"
                     /translation="MTEYNNNFDHELGWDDEITQDSVGFVQLVPGDYQFTVTNMERGR
                     HTPDPQNPGKLPACNKAVVTIQIETSEGIAQLTHNLFLYTTTEGMLSAFFGAIGQKKH
                     GEPLKMNWNTVVGAKGVCRVNKRKGTGKYADQEYDNIKSMIYADEVDWSKVLNASQPT
                     QNTYQQPQQPTQQQPQPQQGSFFQGNTTQPQGGWGGF"
     gene            54487..56070
                     /locus_tag="VC394_000489"
     CDS             54487..56070
                     /locus_tag="VC394_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEP5993983.1"
                     /translation="MQLRPYQEEARSAVQKEWEEGRKRTLLVLPTGCGKTIVFSKIIE
                     DRVRLGERVLVLAHRSELLEQASDKLMTATGLGTALEKAENTSLGSWFRVVVGSVQTM
                     QREKRLSKFPANHFDTIIIDEAHHAISDGYQRVLQHFDSSNVLGVTATPDRGDKQNLG
                     KYFDSLAYEYSLVDAIKSGYLSKITAVTIPLSLDLTTVSQQAGDFKASDIGTALDPYL
                     EQIADEMVKQCANRKTVVFLPLVKTSQKFRDILNQKGFKAAEVNGESKDRAEVLEDFD
                     NDKYNVLCNSMLLTEGWDCPTVDCVVVLRPTKVRALYSQMVGRGTRLAPGKENLLLLD
                     FLWHTERHELCRPAHLIASSPEVAQKMVENMTEETEVAFELIEAEEVAAKDVVAEREE
                     ALAKQLAEMRKRKRALVDPLQFEMSIQAEDLADYVPAFGWEQSPPSDKQLEALEKFGI
                     YTDDIGNAGKATKLLERLVKRRETGLTTPKQIRTLERYGFKNVGMWKFEVASNMINRI
                     AASGWRLPKGINAREYQPE"
     gene            56083..56277
                     /locus_tag="VC394_000490"
     CDS             56083..56277
                     /locus_tag="VC394_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5993984.1"
                     /translation="MENMNEQIEKFINDFVNEAIEKSDTYADAILYVNKIASLTELGQ
                     VIKKAIQDKIGECALNSKIN"
     gene            56300..58543
                     /locus_tag="VC394_000491"
     CDS             56300..58543
                     /locus_tag="VC394_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEP5993985.1"
                     /translation="MAENDFNLLPLLDYINPATVDYRTWVQVGMALKHEGYTAMDWDV
                     WSQSDSRYKKGECFAKWDSFQGNGFGTVTGATITQLAKENGWTSDYRTSDEAHELGWD
                     DTIDRDYKIVDKNWVEAKEIKEPTNWSPVHDLITYIDTLFESTDKVGYVTKTYPIETD
                     EGVVYKPTKGDYARTAGELIQELQKYGNDIGAVLGDYKEEAGAWIRFNPLDGNGVKND
                     NVTDFRYALVESDSMELGKQYALFKELELPIATLVHSGKKSLHAIVKVDARDYQEYRK
                     RVDYIYQICKKNGLDIDTQNRNPSRLSRMPGIMRNGHKQFLIDTNIGKTNYEEWYQWI
                     EDLNDDLPDPETLADEWDNLPELAPELIKGVLRQGHKMLIAGPSKAGKSFALIELSIA
                     LAEGKEWLGWDCEQGKVLYVNLELDRPSALHRFKDVYDAMGLPPTNVANIDIWNLRGK
                     TVPMDKLAPKLIRRSLKKNYQAVIIDPIYKVLTGDENSADQMAHFTNQFDKVATELGC
                     SVIYCHHHSKGSQGGKKSMDRASGSGVFARDPDALIDLVELELTDELIKQRVEKASAK
                     VYQKALQEQNLSYYQHEVTLDDLESRYQMQQHFDKAIPDIMLKQPYLNEIKRITHAIE
                     ISTGWRVEGTLREFAKFQPVNMWFSYPVHEVDTTGVLADIQLEESKPNWQKAKEGRKS
                     KKEKAEERKQKIDTAYSALFDGFTPVSLSELQEYLDSTEKTVRNHLKEHGGFDIKNGI
                     VYQKKTE"
     gene            58835..59017
                     /locus_tag="VC394_000492"
     CDS             58835..59017
                     /locus_tag="VC394_000492"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5993986.1"
                     /translation="MQIEFFKNGKSDIELEYLKNEVNGFINDLREDGIKCLDIQFIFD
                     EDEIIVFVKYEGLIDD"
     gene            59010..59405
                     /locus_tag="VC394_000493"
     CDS             59010..59405
                     /locus_tag="VC394_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HEP5993987.1"
                     /translation="MIDFFIPMKKIPTTTHQQKKVRVVNGKPQFYEPDSLKEARAMFM
                     SKFAPHAPEKKMTGPLRLTVKWLFPKIKKSVDGQYKDTKPDTDNLNKLPKDCMTALGF
                     WNDDAQVASEIIEKFWADTVGIYVKVEEL"
     gene            59402..59626
                     /locus_tag="VC394_000494"
     CDS             59402..59626
                     /locus_tag="VC394_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5993988.1"
                     /translation="MKIDYINFFERVVPDWMRESNVKMQEVGFNTEAYWIWANQSIVT
                     ICETYGNDSLINGQFHLIWEWLESKTKESS"
     gene            59629..59814
                     /locus_tag="VC394_000495"
     CDS             59629..59814
                     /locus_tag="VC394_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5993989.1"
                     /translation="MVKHVVRVYNKGITATYAIYDKNLFQEHEFVTKIEAMQFIRRLE
                     LANDKRATEYFMREVDE"
     gene            59811..60062
                     /locus_tag="VC394_000496"
     CDS             59811..60062
                     /locus_tag="VC394_000496"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5993990.1"
                     /translation="MILTKDLAKKHLGCYVDRYRRNDVINGMFPKRIVELSDGRLYLR
                     NPIGVYTKIEEDLDIDYIFKPIKVLEFLSETEVNDDTKI"
     gene            60046..60450
                     /locus_tag="VC394_000497"
     CDS             60046..60450
                     /locus_tag="VC394_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5993991.1"
                     /translation="MIPKFRAFNKKTKKMYSIDGFKSSERKIYRCSLADDEFRSGRLE
                     TFHFVEDNLDDYILMQSTGLKDKNGIEVFDGDIMFYEQDCYQYTLVKYDKDKLAFVLY
                     DGCERLYHELWEPGEVIGNIYENPKLLESVEE"
     gene            60447..>60710
                     /locus_tag="VC394_000498"
     CDS             60447..>60710
                     /locus_tag="VC394_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5993992.1"
                     /translation="MTTDELLQNLRDDLNKIMNVLKNDWKALLFLAIAIFGMMVTVSY
                     FSYRDARQYYESQITGLRTQLSRTQKQLKRASEDRARQTKRIAE"
BASE COUNT        18856 a        10780 c        12870 g        18204 t
ORIGIN      
        1 ctatttaaaa aataaagaaa tggcaaaaaa cataaaatac ttgcattttc attgtaattt
       61 gctacaatag taaggtaaag ttagactgta tggcctaccg tctatctata aaatatattt
      121 tattggaggc ttttcctaaa atggcaaaag aaaaatacga tcgtagtaaa ccccacgtta
      181 acattggtac aatcggacac gttgaccatg gtaaaactac tttaacagct gcaatcacaa
      241 ctgtattggc acgtcgcttg ccttcatcag ttaaccaacc aaaagattac gcttctatcg
      301 atgctgctcc agaagaacgc gaacgcggaa tcactatcaa cactgcacac gttgagtacg
      361 aaactgcaac tcgtcactat gcgcacatcg acgctccagg acacgcggac tacgttaaaa
      421 acatgatcac tggtgccgct caaatggacg gagctatcct tgtagttgct tcaacagatg
      481 gaccaatgcc acaaacgcgt gagcacatcc ttctttcacg tcaggttggt gttaaacacc
      541 ttatcgtgtt catgaacaaa gttgaccttg ttgatgacga agagttgctc gaattagttg
      601 agatggaaat tcgtgacctt ctttcagaat acgatttccc aggtgatgac cttccagtta
      661 tccaaggttc agctcttaaa gctcttgaag gcgacactaa atttgaagac atcatcatgg
      721 aattgatgga tactgttgat tcatacattc cagaaccaga acgcgacact gacaaaccat
      781 tgcttcttcc agtcgaagat gtattctcaa tcacaggtcg tggtacagtt gcttcaggac
      841 gtatcgaccg tggtactgtt cgtgtcaacg acgaaatcga aatcgttggt atcaaagaag
      901 aaactaaaaa agctgttgtt actggtgttg aaatgttccg taaacaactt gacgaaggtc
      961 ttgcaggaga caacgtaggt atccttcttc gtggtgttca acgtgacgaa atcgaacgtg
     1021 gtcaagttat tgctaaacca ggttcaatca acccacacac taaattcaaa ggtgaagtat
     1081 atatcctttc taaagacgaa ggtggacgtc acactccatt cttcaacaac taccgtccac
     1141 aattctactt ccgtacaact gacgtaacag gttcaatcga acttccagca ggtacagaaa
     1201 tggttatgcc tggtgataac gtgacaatca acgttgagtt gatccaccca atcgccgtag
     1261 aacaaggtac tactttctca atccgtgaag gtggacgtac tgttggttca ggtatcgttt
     1321 cagaaatcga agcttaattt taattaagtt cccagaatag caatttaagt cagacaactt
     1381 aagtttgaag aagcccccaa ttcgggggct ttttgctatc ttaaactcca taaaatgctg
     1441 ataaataaga atgaaaagct ttcagaaaaa gggtttttat ttgtgaaaat attcctaaaa
     1501 tataagcaat tccttttaat cgtcggtcat ttgtgctaaa atagaagata taaataaaaa
     1561 aagaagaggt atgtgatatg tcacgtaaac caattattgc tggtaactgg aaaatgaaca
     1621 aaaaccctca agaagcaaaa gcattcgttg aggctgtagc aagcaaatta ccttcaactg
     1681 accttgttga tgttgccgta gcagccccag ctgttgattt ggtaaccact attgaagctg
     1741 ctaaagattc agttcttaaa gttgctgcac aaaactgcta ctttgaaaac acaggggcat
     1801 tcactggtga aacatcacca aaagtgcttg ctgaaatggg agctgactat gttgtgattg
     1861 gtcactcaga acgccgtgat tacttccatg aaacagatga agacattaat aaaaaagcca
     1921 aagcaatctt tgctaacggt ttaactccaa ttgtttgttg tggtgagtct cttgaaactt
     1981 atgaagctgg taaagccgtt gagtttgtag gtgcgcaagt ttcagcagca cttgcaggtc
     2041 tttcagcaga gcaagtagct tcacttgttt tagcttatga gccaatctgg gctatcggta
     2101 ctggtaaatc agctactcaa gatgatgctc aaaacatgtg taaagctgtt cgtgacgtgg
     2161 tagcagcaga ctttgggcaa gaagttgctg ataaagttcg cgttcaatac ggtggttctg
     2221 taaaacctga aaacgttaaa gattacatgg cttgtccaga tgttgacggt gcccttgtag
     2281 gtggtgcatc acttgaagct gatagcttcc ttgctttgct tgatttcctt aactaagtca
     2341 tctaaaaaag aaaaggttaa atccttttct tttttagatg acttaactct tactatgaga
     2401 gaataaaggt gattctacag gcaaatgcat tagcctaata gcggtttaat agcttttttg
     2461 ccaatctaat gaaatgatat ttgattggaa atggataaca tctaaatgtc cctggttttt
     2521 gcaaaataaa gccattgaaa ttctgtttaa agcgcaagac accgtctgaa ccatcaaact
     2581 ttccagtaat accaagaaaa ttatattgtt tgatgccctt gtgaattgcc ttgagcatag
     2641 catgctcttg taatagagct gggctataaa atttattaaa tttggggtaa gatcctgaaa
     2701 agaggtagac ggcctcttgt tcggtataga taaaaagact tcccgcaagg aacacatctt
     2761 tagttccgta ctcttcaaga aaatggagag cttcagaaat tcttacctga aaggtttcga
     2821 attggctgga tagttctttt aatctatttt gctttttttc agaatgagga tttttgccaa
     2881 ggtctccttt aactttgttg atgctggttg ctagttgtag ttgtctttgt ttaaggttat
     2941 taaggtaatc ttcaaaatta agagtcgcaa ccataaattc acaagagtct ccaaaactat
     3001 cataaaagtc ctgatagtaa gataatgatt tgtcaaggta atctctacga tcagaggttg
     3061 cttcagtgat ttctttgaaa tggtgtaact catctcgttt cagttgacgt aatttaatac
     3121 caaaagtatt agcttttttt attaaagctt ttcctttttt gctgaaggaa tgggttaaac
     3181 gtgaggaatc aattccttct aactttttaa cgtaatgcca aacaggttca ccctcaggat
     3241 agcctgtttt taaaccgtca tgtttatatc ctaacgccgt taaaaggcta ataaggtcag
     3301 tgtttggatg agaaataggg atgccatcag tatcaaatga ctgataattg tcgtaaggtt
     3361 ttaaaatgag ttcgataacg tcattttgtt tggcatagtc tttaagttga gtgtaaaaat
     3421 gttctaattc ttcgggatga ttggtattag ggcctgcatt gagttccata cgccaacctc
     3481 cagccacttt ttggctaaag accatagcag caacctttag ttcaccatct ttttcaagtc
     3541 caagaaattg tggttttgcc cctcttttag ccatcaagct ggccatttct gaggtttgga
     3601 taaaggaatg atggacgaga ctgtggcagt aatggtcaaa ttgttcttgt gaaatttcaa
     3661 ttagtgccat tagttgtgct ccttaatttt tttcttaagt tataggtcaa gatggctaag
     3721 cttgaaacaa ggccgacagg aatgttgaat tcacctgcaa attcttcgat ggtggggttg
     3781 agtctagctt tgaaatgata gagtccacca tcttgttgat tttccacacc tcctaaatta
     3841 tgccagcggc agcctctttt aaaagcttct ttagccgttt cataccaggt taaaagagga
     3901 gcttgataat tgcgataatc atcgtccatt cccgcataaa gattttcaga agtctcgcca
     3961 taaatcaagg ttagggtggc ggctagtggg atgcgggttt tgcctgcagc gagctgctcg
     4021 cttaaaatgg ttaaatcttt ttgtagccgt gcaatggttt tttgattttc tgctactttg
     4081 ctaggtttac tcttatctgt taatcgtgct tgttctgcta aggccttgtc caactgttgg
     4141 atcaagagtt ttttttgttc aggtaagtct aaactagcca tagtaatgta ggactgacca
     4201 gcataaatat ctagtaattt ttgatagtag gatttgcctc gaaggataat gccttttcga
     4261 ttctcggttt ttttcatcaa ggctgaaaag tcgtctagca attcactgcc accaatcgtg
     4321 acattaacac ctttattggt agcggtacga atggattgct tggctttttt gggcaggctg
     4381 tcaaagtcaa aatccttggc gtagatgttg gcttgtattc ttggttgaat agtgtcttcg
     4441 agttcttttg ttctgccaga ccattcgata ccaagttttt ttagaaaagc aataatagag
     4501 agtgtcacat cattttcttt gctttctttt ccttctaaag tttgtttgat gactaaactt
     4561 ggatcaattt tgacaaaaag cgctcgtttg ctttttccga aagttttgag cgttttaatg
     4621 acgaaatcaa ggagttcaaa attggcgtag tccatgattg gcccacgtgg gatataaatc
     4681 atagtgaaac caagaggaag ttttctgatt aaacaagcag cagcagcaac ttgtacccca
     4741 ttttcataaa agctgatgcg ttcgtgtttc caattatctt tgacttttct ccactttgaa
     4801 ctttgaagta agccagcttg tggctgtgct aagacaaact gatcatgttc ttcaggagat
     4861 attccgattt tatatgtata cattagttta atacccactt gcgaatagca tgtgccacac
     4921 cactgtcatt gttggatttg gtgatgtatt tggctatttt tttcaattca ggaaccccat
     4981 tttccatgac aacagggttg gcaacgactt ctaacatagc acgatcattt tcagcatccc
     5041 cgatggccat ggtttgtgac atatctagcc cgagtttttt agctaagtgt ttgatggcat
     5101 tgcccttgct gacggttttt ggcatgaact ctaaataaaa aggggttgac ttgacgatgg
     5161 tgtacttatc aaagaaatgt tgaggaattt gcttgatggc cgcgtccaaa aggtcaggtt
     5221 catcaatcat catcattttg atgatttctt tattggtcat ttcttcggga gtgcgataga
     5281 aaataggcat gtttaccaaa gtcgattcat gaactgtgta ttttccaata ttccgattgg
     5341 cagtgtaaat accttctttg gtaatggcat gcatgtgaac tcctaatttt cggctcaaaa
     5401 attccgtttc cagataatcg tcgtaagtca tcaattcttt aacaatttct tcgccggtct
     5461 cggcatcttg caccaagcca ccattaaaag tgataacgtg gtttcctttg tggttgagtt
     5521 ctaattgttc gagtaatgaa atcactccgg caataggtct gccagtagca atcaccatat
     5581 gaaccccttg ggctttagcc tcttggacag cttgaaaaac atcatctgta attcgacggt
     5641 catcagtgag taatgtgcca tcaatatcga cagcaaccag tttgatagac atggcatcct
     5701 ccttgttttt ttgtggttta tcttaaaaat gttctccctc ctatcttact aaaaataggt
     5761 gcttttgtca tgggatgaga aaggaaactt aaggaaaaac gagagaaaac gttttagttt
     5821 agctcaatcg cctctgtagt aactaaaaag attgaagaaa gtactctttc ttcaatctta
     5881 gaatggagac aaacccagtt tgatgctcaa aaagcatgaa ctgctctgct gatttgacaa
     5941 ctgatttctt atttctgcgt gtgacgtgag ccaaaacagc tgctttttgc tgtggcaaaa
     6001 gtggtgtcaa tactggcagt gacgttacgc ggaatttttg agaccctagg ctcaaaaata
     6061 aagcatagaa ttccaaagga agtcgctgcc ataagcatca cctaagaaaa gtatcaaaaa
     6121 agactttttc tgcaatctta aagggcttta ctgactaaag tgaaaatgcc cattggaaat
     6181 ataactcata aaagtttcct tgctaggagc aaagaggtca tcgagctcta acatttcctt
     6241 tggaaaataa aagcgacggt ctccgtaggt tgtccctgtt aatgctttga caataggaga
     6301 taactgagat aattcagcaa ggctcccgtc tttttgtatc atttcaatct gagttcttgg
     6361 attttcgagc tctggtcgat aaatatcata aggcaggtcg aagttgatat ggataccggt
     6421 gtaataatct ggatcaaagc caactgattc gaccaattgg cgcaagcgtt ctaactctcc
     6481 tcgggaatct tggtcaaagg taacagactt cagaattttt cgattaataa agcgactggc
     6541 caaatctgat aagatatggt cttcgcttgc catccagact tggaagtaag tattcatgac
     6601 tccgtcatct agggctatat aatctgctaa attagccttc ttttcaaaga aggggatgag
     6661 tccaggagct gttttttgga aataagcctg ttgctcagga tataagtgtt gggctctttt
     6721 gaggagattt tgtaagatga gctcaacagc acggctggcg ggatgaaaat agacctgcat
     6781 gtacatttga aaacgactga caatatagtc ctcgacagca tgcataccac tatgttcaaa
     6841 gacaatgcca tcttcaacag gtcgaatcac acgtagaatg cgcattaaat caaactgacc
     6901 ataattggca gcagaaaaat aagaatctct taaaaggtaa tccatacggt cgcagtcaat
     6961 ctggctggaa atcagttgca caacttgctt gttaggatag gtatgattaa tcacgctggc
     7021 aactttgtct ggaaaatcaa gagcatgacg caccaaaatg gcattgattt ctgtctcagg
     7081 attggtaatt atctcctgag taaaggcctc gtgatccgta tgaaaaagaa cctcaaaggt
     7141 atgagaatag gctccatgac caatgtcatg taagagggca gccgtcatag tgaccagact
     7201 ttcatcttta ttccagatat cagcgtattt ctcttcaaaa atagctgtca cccgacgggc
     7261 aatttcgtaa acacctaggc agtgagaaaa acggctatgt tctgcgccgt ggaaggtgaa
     7321 agctgttgta gggacttgtt tgatgcgtcg taagcgttgg aattctttgg tattgataag
     7381 gtcataaatt aatggattat caatatggat atagttgtga acagggtcac gaaatacttt
     7441 ttcattcata atttttatta tatcaaaaat tgataaaata gggagaaatt gatagaataa
     7501 acagacgagc ttagaataga aagaagagtt atgaaattac agttaaccaa ccatattcgc
     7561 tttggagatg agacagagat tattcaagaa attcatgatt gtgagtggcg agaaaaaggg
     7621 ggctatcagt acctcatcta tcaaaacact gacaaggaaa aggttgtcat caagtataat
     7681 gagacagaac tgaccatgag ccgtttttcc aaccctcagt caatcatgaa attttttgcg
     7741 ggcaagaagg tcctgattgc tctacctaca ccgatggggg ttcaacaatt tttgacagat
     7801 accagtcatt accacttaga ttgttcttgt caaaaattgg accttcatta tcatttgctt
     7861 caagctcaaa cagaaatgct atttgcaagc tatcatcttg agctgagttg ggattaacta
     7921 aaaagatagc agtaaatcat ctgattaggg attattcttg ttttttagtc aggtttttac
     7981 tataatggtt attatatcaa gggagtagca gacggctagc cgtgattgta tcgtcaatac
     8041 ggaatttcgg tttccggtac aatcttaaac agcgagactt gtttaagaaa ataaacaggt
     8101 ctttttattt gaacaactaa aataaaaaga ccaaggaggt actaatttgt ctaaagaaca
     8161 aagacatgaa gcattttaca cgcaaagtga agagaccgta ctggctcaat tagagaccag
     8221 tcgcgaaggt cttacgtctg cccaagcaaa agagcgctta gcagagtatg gtcgtaatga
     8281 gcttgatgaa ggcgaaaaac gaagtttatt tatgaaattc cttgatcaat ttaaggattt
     8341 gatgattatt attttgattg tggcagccct tctttcagtt cttacagagg ggatggaagg
     8401 tttgacagac gccattatta tcttggctgt tgttatccta aatgctgctt ttggtgttta
     8461 ccaagaagga caagctgaag ctgctattga agccttgaaa tcaatgtcaa gtccactggc
     8521 tcgtattcgc cgtgatgggc atgtgactga gatagactct aaagaattgg tccctggaga
     8581 tattgtcttg ctagaagcgg gagatgttgt ccctgctgat ctgcgtcttt tggaagctaa
     8641 ctccttaaaa atcgaagaag cagcgcttac tggagaatct gtaccggttg aaaaagactt
     8701 gtcaacggct gtttcagaag atgccggtat tggtgatcgc gtcaatatgg gatatcaaaa
     8761 ctcaaatgtg acatatggtc gtgggattgg ggttatcact aacactggca tgtacaccga
     8821 ggttgggcat attgctggta tgcttgctaa tgctgatgag acagatacac cattgaagca
     8881 aaaccttgac aacctttcta agattttgac ttatgcgatc cttgtgattg cagcggtgac
     8941 ttttgctgtt ggagtcttct tgcgtggtca acacccgctt gaaggcttga tgacttcagt
     9001 agcgcttgcc gttgcagcta ttccagaggg acttcctgcg attgttactg ttgtgctttc
     9061 tcttggaact caagtacttg ctaaacgtaa cgccattatc cgtaaattac cagctgttga
     9121 aacgctagga tcaactgaaa tcattgcttc tgataaaacg ggtaccttga caatgaatca
     9181 aatgaccgtt gaaaaagttt ataccaacgg gacactccaa agttcatcag ctgatattgc
     9241 ttttgacaat acgacacttc gtgtaatgaa ctttgctaat gatactaaag ttgatccttc
     9301 agggaagtta attggtgacc caacagaaac agctttggta cagtttgggt tagaccacaa
     9361 ttttgacgta cgtgaggcta tggtggcaga acctcgtgtg gcagaattac catttgattc
     9421 tgaccgtaag ttgatgtcaa ccattcataa acaagctgat ggcaaatatt tcatcgctgt
     9481 taaaggtgcg ccagaccaat tgttgaagcg tgtcacacag attgaagaaa atggacaaat
     9541 tcgtcctatt acggatgctg ataaaaaaac cattcttgat accaataaga gtcttgccaa
     9601 acaagctctt cgtgtcttga tgatggctta caaatattct gatgctcttc caacactgga
     9661 aacagaaatt gttgaggcta atcttgtctt ctctggtttg gtagggatga ttgaccctga
     9721 acgtcctgaa gcagcacagg ctgttaaagt agctaaggaa gctggcattc gtccaatcat
     9781 gattactggt gaccaccaag atacagcaaa agctattgcg aaacgtcttg gtattatcga
     9841 agaagatggc gttgatcatg tctttacagg tgctgaactc aacgaattat cagatgaaga
     9901 gttccaaaaa gtctttaaac aatactccgt gtatgcccgt gtatctcctg agcataaagt
     9961 tcgtatcgtt aaggcttggc aaaatgaagg caaagttgtt gccatgactg gcgatggggt
    10021 taacgatgca ccatctctta aaacggcaga tattggtatt ggtatgggaa ttacaggtac
    10081 agaggtttct aagggagctt ctgacatggt tcttgccgat gataactttg caaccattat
    10141 cgttgcggtt gaagaaggac gtaaggtctt ctcaaatatt caaaaaacaa ttcaatattt
    10201 actttctgct aacatggcag aagtctttac catcttcctt gcaaccttgt ttggttggga
    10261 tgtgcttcaa ccagttcact tgctttggat taacttggta actgataccc taccagctat
    10321 tgctcttggt gttgaacctg ctgagcctgg tgtgatgaaa cacaaacctc gtggacgtaa
    10381 gtcaagcttc tttgatggtg gggtgaagga agccattctt taccaaggtg ccttccaaac
    10441 cattttggtg cttggggttt atggttttgc cttgatgttc ccagaacaca caagctacca
    10501 tgatgtccat gcagatgcct tgaccatggc ttatgtgacc ctcggtttaa ttcagttggt
    10561 acatgcctac aacgtgaaat ctgtttacca atccatcttt actgttgggt tgttcaaaaa
    10621 caaattgttc aactattcta ttccagtggc ctttgtagca ttgatggcaa cagttgttgt
    10681 tcctggattt aatcaattct tccatgtgac acacttgacg atcacacaat ggttagtggt
    10741 tattatcgga agtctcttga tggttgttct tgttgaattg gtgaaagctg ttcaacgatc
    10801 acttggtcaa gatgaaaaag caatttaata gacgctaaaa ggtttgagac aattgtctca
    10861 aacctttttt tataatcgtt acaacgataa ggactattgg tatgcctgac attaaaaagt
    10921 tgattcttgc caattgactt gacagtcaaa aatttgttgt ttggcttctt gactgtgacc
    10981 ttcaatatca tcgattaaaa tcttagtagc ctgttcgcct tcttcatagg cgggttggat
    11041 aatagttgtt agtgttggtg aagaaaattt tgtccactca atgttatcaa atcctactag
    11101 tccaatctct ggtatattaa atttaagaga cttcatagct gtaaatactt ttggaagagc
    11161 ccaacaattg gggacaaaaa ccaaactctt tttagtcagt gatccttgca aaaactgagc
    11221 gattgcctct gaagaagtct gattttcatc aataatcatt tcttgatgag taagatgatt
    11281 agcttctaac acatcaatga agcctgaagc gcgttcaata cgagtgctaa gtaaattggg
    11341 gttacccgtg atcatgataa aatgttcata acctttatcg atacattgtt gaatggtatc
    11401 ataaacagca tcataattat tagttttgac ccagtttgtg cgatgttcgt aaagttggct
    11461 atcaaaaaag accacttttt tcttcttaat atcgataatg cgagaatact tcctgaaatt
    11521 agaggtaggc tgaatgataa agccatccac tccgaggttc agcatctttt caatgagttc
    11581 atcttcgcga gaagggtcgt agttgctgtt tccgatgata atttggtaac caaattcctg
    11641 ggctttgctt tcaattccct taacaatttg attggaaaaa ctattggtaa tatcaccaat
    11701 aaccacgcca atgagtttag tagatttggc gtttagactt ctggcagcaa tgcttggttt
    11761 ataattggta gcttcaatcg tgcttttaat gcggttttta gtttcttcag acatcttgtc
    11821 aaacctacca tttaagtaga aagaaactgt tgtttttgaa gttttcgcta attcagcaat
    11881 atctttgatg gttactttgc gctgcattgc atacactcct ttaaaatagt aatggttagt
    11941 catattataa cataaggact cgtaaaagat ttagtgtttc aatcgaataa tctcattcgt
    12001 gatatggttg ataacaacca cctttcctct gattttatga ccatttagga gaactagttt
    12061 ttctccttta attaagtcct cttggagaac gcagatactg tagtcaaact gctttccttt
    12121 taaatgccag gaaagggcgg tctcaattgg gttgcgtttg cctgtttgaa gtggtgttaa
    12181 gggagtaact ttggtatcgt cagggactaa cagggtactg gtgcaaccct tgtctttaaa
    12241 gggttttcgt ttgatgaatt tgtgactagt caggtattgg ttgtattttt ttgacaaaat
    12301 cgtttcttcg atagtgaaag ggcagtcact gattagtctg aggtcactaa ggtgatctgt
    12361 ctttacttct aagtgattat caagaataaa ttgactgaca agacagtggt taccttgagc
    12421 ctgtatcgta tcaatgataa gaaacattcc tggtggtaag atgagaattt gtcggtcatg
    12481 ttggtagttg ttcctatcag agaattgcgt ctggtagcta ccttcaataa agtaaacgtg
    12541 tccattttgt cgaaggtgac aataattgga tttggggtaa gaaaggtaag cccaggagct
    12601 attaatctgc tcaggtaatt gttcttctaa gaaggcggta gaatgatggc tagcgctttt
    12661 tagggcatag cgtaatggtt cttctttgta agtgtatcgc ccagcatcac agaaaagtgg
    12721 ttgtccttta taatagagac agatactatt ttggtcgcta tgggtatggg aactaccgat
    12781 gggcccattt ttgaaaaaaa gataataatt ttcttgattg atggtaatgt gcccggaatg
    12841 ttcaaagtga tgcgcaaaag ttggtaaggt ttggacgggt aactgttcaa aagtgtggac
    12901 acctttttct cctagaagga gaagactatc gatatcaagt gtgggaaaag cagcagcttt
    12961 taagtgtggt tcttcaagga tagtggctgc tagtgttaga atatctctgg tatcggtaaa
    13021 gtcactgtct cctaagggga tctgtttgtg gtcagggcca gtcattttga ggagataatc
    13081 actcatggca agcaaagtag gtctaagctg tggaagataa tcagggacta gagcagttaa
    13141 ttccagaagg gctttgagta cttccacatg atacatggtt gattgttcaa attgactgcc
    13201 gtcttcgaga atttggagtg cgatttgttg cgtcaattct tttctagcga aagctgttgc
    13261 tgcagcaata tcaaggtcag aaccaaagta ggcatcagct aataaaatcg cagtggtttg
    13321 caagattccc caattgctga gactgtattt atcaaggtag ttggcatgga gaaagtgaag
    13381 ttgtttttcc agggaagcta ggatgagcct ttcctcttgc ttggttaagg cgtcaaaaag
    13441 attaaggtaa atcaaacatt tgacccaagc aaaacaacgg atgccagtat ctaaggttct
    13501 agtagctaac cccttaggat ctaaaggaat agctgattca atccaattga gaatgaaacc
    13561 tttagctgtt aagagatacc gttcatctct ttcaacaagg tagacgagga ttaatgtttg
    13621 gagataggtc tgtcgattta acataaaatt ccactcagga tcatctgtga cagcctcttg
    13681 ccaagtaatg ggatctaagt ggtaagggat gtggcagggc tccatatccc aattatcttc
    13741 aaataaaaag gtattggtta agagtaggtc agctatcctt tttcgatcag catagctgtc
    13801 agcctgatag tctagcaggt aattcctgca aaagtttgga ttgactgttt ctttaaagcg
    13861 tgtgaaggca agagacatag gacgtttcct ttccaaaaga aaagagacct gttaatgtga
    13921 ctaggaaata gttcctaaac ccataacaga atctcagtga caactaagct aaaataccca
    13981 accaactgcc aataacacca atgataacag ttaaaatgac caatttgtag gttgtccatt
    14041 ttttgttttt aataaggtaa tacatgagta aggtaaagag agctggtaaa agtgcagggg
    14101 caatcttatc aagcattcct tgaatagtca caaatttttg tgctgtattg gccgcatcca
    14161 ccttgccagc cgcaaaagta atgggaacag ttattttaac agaagtagca gctaatccag
    14221 cgataacggt aacaccgaca atatttgcca tacgagatac tatcgccatt tgttcgctta
    14281 gtttatcaat aaagcttgtg ccgaccttgt agccatagag accagaaccg attttaatag
    14341 cagttaggat aatattcatg gctaggaaga agaaaattgg acctaacact aaaccatcac
    14401 tagctaagga agctgcaatc gttgaaaaga gtggcgccaa gcaaaattgt gataaggagt
    14461 caccgatacc agctaaaggc cccataagtg ccattttgat gtttcgggtt tcttcttcag
    14521 gacgatcatt ttctaacatg accaagtgca gactggtgat gaaaggaagg aagtgagggt
    14581 tagtattgta aaactcacag ttatcttcta aagcttgata taagcccttt ttatcactgc
    14641 caaaatgttt tttcaaggca ggatagatga cattggcgta tccgatacct tggtaattac
    14701 tgtaattaaa cccattttgt aggaagaaag ctctcagagc tgttttgagg taatctttct
    14761 tggttaattt attagatcca gtcatcttgt tggcctcctt ggacagttgt tgcatcgact
    14821 tgtttaggtt tgttgtaaaa ttcattcaag gcgaaaatga taccaataat ggcgatacca
    14881 atggttggaa tttggaggta ggctgcacaa acataaccaa tcagtacaaa aggaatcaac
    14941 tccttcttgg ccataactga taagatcatg gcaaatccga tagcaggtaa cattttacca
    15001 gcaacagtca atccattgag caagacaggt gggatataat caaccaagtg aaggagggta
    15061 tccattgaca aggcacctaa taaaccaagg cctaatccca taaaagcgaa agcccagata
    15121 gtgccgttgg cagcgaattt gaagcctcta atattgcctt tttgcaattg ttttttagcg
    15181 gtttcaggag ccccagcaaa agcggtatag gcgaatgtct gaagaaattg gatgacaact
    15241 gcgattggtg ttgataaggc tagggccgct tctggggtaa ctttaccagc actggtaata
    15301 gccatcaagg taccaaagat accagggccg ataggattag gaggaacggt accaccggca
    15361 cccactccaa atcccatata agctaattcg gagatagctc ccatgctaag tgcagttggc
    15421 aagtcaccta agatcatccc tacaccaaaa gacagaataa tacaacggtt ggtgtagatg
    15481 ccaagtaaca ttccgctgaa acaaaaggct gtccaaagac caattaaaag tgcctgtaac
    15541 aaattgatat ccatatcaat ctcctttatt cattaaatat aatctaagat gtcaacgtca
    15601 gaggcgctat tgccagctgg agttgttttg gtgttaaaaa ccacgtggtg gtcatgggct
    15661 aagcaacgaa tagcagactt gtctgtttct cccagcgaaa taaactgggt aatcgcaacc
    15721 ttatcatcag ttttatgaat attaccgata ttgatttctg tgatgggaac acccccttca
    15781 actaggcgtt tggcatcttg caaatctttg accacgatga agatactttg agcaggactt
    15841 gctttatgaa tgatgtcaat cactttttga atggagaaaa aacgaatggc aatactggat
    15901 ggaataactg ttttcatgag ggattgttga atcttatctt cagaaaccgc atcgttagcg
    15961 acgataacgg tgttgcagtt gaggaatttg acccataatt gcccctgtcc gtgaatcaag
    16021 cgctcatcaa ctcgcgtcat gataatgttt ggttgtgtca taaaatctcc tttttaaatg
    16081 tcggtatcgg tgtttgttac cagtaagggt tccagtcttt gtagaaacga agcagagctt
    16141 caagatagta atagtctccc cagatatttc cctcgtcaac cccttttcca gagtgccaag
    16201 aatagactcc gtgaagtagt agaggagcgc caggattgag atttttgtta gcataatcct
    16261 taatcaaggc tcttagcatg ctgtgcatgg cagcttcata tgttttttta tcaggatcat
    16321 gatctggtaa ggtttttaac atttcatgaa taccacatac agcaatggcg gttgctgaac
    16381 tatcacgaga ctgctcgcta ccatctccaa aaattaaatc ccagtaggaa acctggtctt
    16441 taggaagacg gttcatgaaa taatgggtca tgcccttgaa gagttgaatg agttcaggtt
    16501 cttttaggaa acgataggtc aaaggaatac cataaatgcc ccaagcttgc cctctggccc
    16561 aagcagaatc atcgctataa ccttgtcggg tgaccccttt aataggatcg cctgtttctg
    16621 gatcaaagta gaaggtgtgg taagcagagg cgtcatctcg gatgacatga ttggctgagg
    16681 cgtagaaatg attgatcgcc atgtcacggt aacggttgtc tccagtttct tggctagcaa
    16741 aaaagagtaa ttggatgttt aggagacaat caatgattaa acggtaatct tcttttttac
    16801 ccagcacccc ccaggcctga ataaagccgc ctttgtcttg atagcgttgg attaatttgt
    16861 cggctgcttt caaagcagct tctctagact caggtgtttt taaaagtttc cactcagcca
    16921 tgcaagaagg ggtgtaaaga aagcccaaat catggtgatc tagctcaata tctcttgtta
    16981 ctcgatctag gaaagaaagg tcatttgctt gagccagagc tttaatggca tcatccccac
    17041 cgtattcgta agctaaccaa aggcaacctg tccaaaaggc attggtccat tccgtgttgt
    17101 ccataatggc gtattggtta tctttggtag ctggcgttgg aaagtcttct ttaaaatagt
    17161 ccatattaag ccgaacttgt ttgagggcta agtcaagggc ctgagtaatg tcttcttggc
    17221 ttagaaagtc ttctttggta aatcgttcag gctgcttgat aggttcaaga gcaatggttt
    17281 ttaaagatcg tgtcattaaa tgcctcctaa ctcatcgtct gtcgtaccgt cttcttgctg
    17341 agaaagagtt ttccagtcaa caatgccttc tttagaacat gttataagtc ctgatactaa
    17401 gtcgtcaaaa gaagctgctg tttgtcttgc aaaactagct tcaatcagca tggctaagtt
    17461 aaggccagaa agaacattac acgtcgtgtt tggaagagat tccattaagg ttgcagcgac
    17521 tttaaaaggg gtacctccta gaaggtcaca aagaactaag gtttcctctt ctgggattaa
    17581 agcacgagat aattggtcct gcacatcagc ggcggtcatc tcagtagtaa aatcaattgc
    17641 agttaccttc tcttgtggtc cggcaattaa ctctagggat gagacaatcc cactcgcaaa
    17701 atgtccatgt cccataacga taatagcaat cataaataaa tctcctttgg tttaccggtt
    17761 tactcaaaaa tattgtagcg ctttcaaaat tctttgtcaa tacttttttt aaaagatttt
    17821 tgaaaaagaa aaagtgaacg gttgtgctaa aaaagaatca aaaatggtct tttttgtcac
    17881 tatttaatgt agaaaaatct taatagctat taagtatagg acaagactaa acaagaatga
    17941 cagtttggat aagagcactc ttttaaattt tgaaccgttt tcattaaaat gattgacaaa
    18001 actagtttac tttgttatag tcttggtaaa caggtttact taatctaata acaaaaagga
    18061 gaatcctatg gaaaatatgt tttcgttaca aggtaagatt gccttgatta ccggagcatc
    18121 ttacggtatt ggttttgaaa ttgccaaagc ctatgctcaa gcgggtgcaa ctattgtgtt
    18181 taatgacatc aaacaagagt tagttgataa gggggtggct gcctatcgag agttagggat
    18241 agaagcgcat ggctatgttt gtgacgtgac tgatgaagct ggtatccaac agatggttag
    18301 tcaaattgaa gatgaagtag gtgttattga tattctggtc aacaatgcag gtattattag
    18361 gcgtacgccg atgcttgaaa tggccgcaga agattttcgt caagttattg atattgattt
    18421 aaatgctcct tttattgtgt caaaagctgt attaccatca atgattgcaa agggacatgg
    18481 taaaattata aatatctgtt ctatgatgag cgaattgggg cgtgaaacag ttagtgccta
    18541 cgcggcggct aaaggtggct tgaaaatgtt aaccaaaaat atagcctctg agtttggaga
    18601 agccaacatt caatgtaatg ggattggtcc tggctacatt gcgacgcccc aaacagctcc
    18661 gttgagagag cgacaagcag atggtagtcg tcatcctttt gatcaattta tcattgctaa
    18721 aacaccagca gcacgttggg ggacaacaga agacttagct ggtccagctg tttttctagc
    18781 tagcgatgcc agtaattttg tcaacggcca tattctttat gtcgacggtg gcattttagc
    18841 ttatattgga aaacaacctt aatcacacac tagaaagaga ttttatatga aaattgcatt
    18901 aattaatgaa aatagccaag cggctaaaaa tggtattatt tacgatgccc ttacctcagt
    18961 gacagataaa cacggctacc aagtctttaa ctatggcatg tatggcacag aaggtgaaag
    19021 ccagttgacc tatgttcaaa atggactctt agcttctatt ttattgacca ctaaggcggc
    19081 cgattttgtg gtgactggtt gtgggacagg tgtgggagct atgttggcct tgaacagctt
    19141 cccaggggtt acctgtggtt ttgcgagtga gccaacagaa gcatatctct tttctcaaat
    19201 caatggtggt aatgccttgt ccattccttt tgccaaaggt tttggttggg gagcagagtt
    19261 gaatttgacc ttaatatttg aacgcctctt tgcagaacca atgggtggcg gttaccctaa
    19321 agagcgagct attcctgaac agcggaatgc acgtatttta agtgatctga aaaaaattac
    19381 ttacagagat ttgttggcaa ttgtcaaaga catcgaccaa gatttcctca aagaaacgat
    19441 ttctggcgct cattttaaag agtacttctt tgccaatgcg gagccgtcag aattagtcgc
    19501 ttatttgaaa tcagtacttg aacagtaatt ccattttgac gaggaaagga ggtcacatgt
    19561 ctaagttatt attggttggt gaaccgctga ttcgcgttag ccccaatcag tttcagcctt
    19621 tgaccaatgc ctgcgacgct cagctttttt tcggcggttc agaagctaat attgcaagaa
    19681 ctctaggcgg ttttggctta gaagcaaggc tatttacggc cctaccagat aatcctgttg
    19741 gtcacgcttt tcatcagttt ttgaagcaaa gcggtgtgga catgtccttg acggcttggc
    19801 aaggtcatcg cgttgggcta tattaccttg aaaatggctt tggctgtcgg gctagtcagg
    19861 tttattatga ccgctgtggc tctagctttt cggccttaga taaggatagt cttgatttag
    19921 ctgccatttt tgaaggcatt agtcactttc attttagtgg gatcagtttg gcgctgggca
    19981 aaaaaactca ggatctgatt gagatactag cgagagaagc taggaaaagg caagtttgtg
    20041 tttcttttga tcttaacttt agatctagta tgattgcggt agctgatgct aaacgactct
    20101 tttctcactt tgctcaatac gcggatatta tttttggcat ggaacctttg ctgttagata
    20161 gtgacgattt tgacatgttt gataggaata aggcagatac tacgatgatt agagaacgtc
    20221 tggcaggact ttatcagcgc tatcagttac aagctattta ccatacggag cgttcaaatg
    20281 atgcccaagg cagtaatcgt ttcaaggctt atgcctatga tgggcagttt tacgaatcct
    20341 gtgaagtgac gaccccagtc ttgcaacggg ttggaagtgg agatgctttt gtggcgggtt
    20401 tactttatca gttgttagaa ggtaacgaaa agcagcgcaa ccttgatttt gctgtggcaa
    20461 cagctagcct aaaatgtaca gtagctgaag atcagctcta tcacagcgtc caacaagtag
    20521 aagcagtctt acataatcaa cgggatgtcc agaggtgatg atatgggaaa aatagaaatc
    20581 ttaaccaaac ttaaggctaa tcggcttgtc ctggttgtac gaggagagtc tagtgaggaa
    20641 gctttagctt gcagcctggc cagtattgaa ggtggcatta agacaattga agtcacctat
    20701 actaacccct ttgctagtga ggttatcggt caattagctg aacgcttcaa agaagatcct
    20761 gaggtattga taggtgctgg gacagttttg gatgatgtca ctgcccgcca agctatttta
    20821 gcaggagcac agtttattgt tggtcctaat ttcaatcgag cagtggcttt gatttgtcat
    20881 ggttatagca taccttattt accaggttgt atgacggtta atgaagtggt tacagcatta
    20941 gaatcagggg ttgatatggt caaaattttt cctggttcta ctgtagggat atcctttatt
    21001 cgggcaatca aatctccttt gccacaagta gaggttatgg ttacaggcgg agtttcttca
    21061 gataatctca aagactggct agctgctgga gtagatgttc taggcatagg aggcgagttt
    21121 aatcagttag cgagccaaaa acaatataac ctaatcacta agaaagccgc tcattatatc
    21181 aaaagtttgc gacaataatc taaaaaacgt ttttaccttg gctttagtgc tagtaaaaac
    21241 gtttttttat cattcattca attaatatgc ttctgctcat gtttttccag atagtataga
    21301 tataagagca gctaatttaa caagatgagc ctgttgtttg atatatagga tataaacttt
    21361 ttatgatcaa acaaaagtaa taccttttga attcgttagt attgatctga tctaaagtga
    21421 catcacttag aatggactag ctgtacagct tttacagcaa cttatgctaa aatagaaaaa
    21481 aggagaaatg ttgatgatta aaggaattat ttttgatatg gacggtgttt tatttgatac
    21541 agaacctttt tatctgaggc gacgagaaga tttttttaag acaaagggaa ttcccattga
    21601 tcatttgaac tctaaagatt ttattggggg caatcttcaa gaattatgga aagagttgtt
    21661 aggtaaaaat agggatgatg ctatcgttaa ggcaattaca actgactatg acgcttacaa
    21721 acaagcgcat aagcctcctt atcaaaaact gttgattaca gaagtgaact cttgtcttga
    21781 acagttgaaa aaacaaggta ttaaactggc tgtggcatca aactcgaagc gtcaggatgt
    21841 tttgttggcg ttggagacaa cgcaaataaa agattatttt gaaataatcc tagcgcgtga
    21901 agatgtttct agaggcaaac cttatccaga tatctataat aaagcagtac aaaaactagg
    21961 attacaaaaa aaacaactgc ttgtagtaga agacagcgaa aaaggcattg ctgccgccaa
    22021 agcggccaat ctgacagttt ttgccattac cgactaccga tatggcattg atcagagtca
    22081 agctgatcac aagatagatc atttaggaca actgtgtgta aaaatcggtt gttttggatc
    22141 gtgaagtaac gcaactaact gatcattgat gatacttcaa gacacttaag aatacgtcta
    22201 ggactttcta cagaccaata acaagaatac gtttatgtgt ttttactgtt ttttagctac
    22261 ctatttacaa tatgacactg aaaagagtac tatgtttaag ttattatcaa aaaaaccgtc
    22321 gcataagatc acctagaaaa ggattatgat ttttgtgatt tggatgcgat cattagtcgt
    22381 tgaccatctt tatgtggcag gagatatgtt taaccgtggt cattatttgg atttgttttt
    22441 agattggcta gtgagtaaat atagaaggat cataatactg ctttgacaaa ggcagtatct
    22501 gagtccatga gttgcacggg taatgtgact cggttttggt taggtataat aatatttgga
    22561 agtgttatgc tgcttgtctt cgtaggtcca ttgcttatag tcagacttac tttagagctg
    22621 agctgactgt ctagacaaga cgtgatggag atgtgatttc tgaccaagta gaggtaacca
    22681 gaaaagggac ttttacgatg taattgaatt tacttgttgc attcttgtgc atgttaagag
    22741 agaaaaccca gataaatcag gaagtaaaat ggttatgttt gattagaatt gtccttcttt
    22801 ttccttgaag tcttaactta ttttgattat gatagtagta tgactattaa ggcagaaatt
    22861 aaggcactcg ccaaagaaat tggtatctct aaaattggtt ttacgacggc agataacttt
    22921 gattatttgg aaaagtcatt gcgtgcttct gtggaagagg ggcgcaattc aggatttgaa
    22981 cataaagtca tggaggatcg tatctaccct gaaaggcttt tagaatcggc taaaacgatt
    23041 atttcaattg gcgttgctta tcctcacaaa cttcctcagc aacctcaaaa aacaccttat
    23101 aaacgtggaa aaattacacc tagttcgtgg gggcttgatt atcattatgt ggtcggcgaa
    23161 aagttagaca gactatcaaa aggcattgaa gagctgtgtc gagattttcc tttgcaacaa
    23221 aaagcaatgg tcgatactgg agctttagtt gatactgcag ttgcccaaag agcgggtatt
    23281 ggttttatag gaaaaaatgg tttagttatt tctaaagaat atggctctta tatgtttttg
    23341 ggagaattaa ttaccaattt agagatcgag ccagacaagc ctgtcgatta tgattgtggt
    23401 gactgtcgtc gttgtctaga tgcctgtcca acttcttgtt taattggtga tggctcaatg
    23461 aatgccaagc gttgtctatc atttcaaacc caagataagg gaatgatgga catagagttt
    23521 cgaaaaaaga ttaaaacagt tatctatggt tgtgatattt gccaaatttg ctgtccttat
    23581 aacaagggta taaacaattc accagctaca gagattgatc ctgagttggc tcaaccagaa
    23641 ttaataccat ttttgagttt atcaaatgga caatttaaag aaaaatttgg tatgattgct
    23701 ggaggttggc gaggtaaaaa tattttacag cgtaatgcca ttattgcgct agcaaatgct
    23761 catgataaaa ccgcagtggt caaattaata gagattattg ataaaaataa taaccctatt
    23821 catactgcaa cagctatatg ggcattggga gaaattgtca aaaaaccaaa cgatgagatg
    23881 ctggcgttta tgagtcattt aaccttaaaa gatgaagata gccaaaaaga gttggaatta
    23941 atccgtcgta aatggcaagt ttaagccttt tatggtattt ttaagaacct tatgataaaa
    24001 taaaacagat taacaaagat tggagaaatc tcatggaagt ggctgaaatt cgccaaaaaa
    24061 tagtagaaaa taaagagaag ttgactagct tcaggaggtc tctttgactt agatcgtttg
    24121 gaagaggaga ttgctcttct tgaaaatcac atgactgagc cagacttttg gaatgacaat
    24181 attgcagcgc aaaagacatc tcaagaacta aatgagttaa aaggtaagta tgatactttc
    24241 cataatatgc aagaattatc tgacgaaaca gaactcttac ttgagatgct tgacgaagat
    24301 gattctttga aagaagaatt agaagaaaat ctaatgcagc tagataagat aatgggtgct
    24361 tatgaaatga cgcttctctt atctgaaccc tatgaccaca ataatgctat cttagaaatc
    24421 catcctggtt caggaggaac tgaagcgcaa gattggggag atttactctt acgaatgtat
    24481 acccgttttg gcaatgctaa tggtttcaaa gtagaagtat tggattatca agcaggagat
    24541 gaagctggta ttaaatcggt taccctctct tttgaaggcc ccaatgctta tggtttgttg
    24601 aaatcagaaa tgggagttca tcgcctcgtt cgtatatcac cttttgattc agctaaacgt
    24661 cgtcatactt catttgcttc agttgaagtg atgccagagt tagatgatac gattgaagta
    24721 gaagtacgtg atgatgacat taaaatggat actttccgtt ccggaggagc tggtggtcag
    24781 aacgtcaata aagtatcaac aggtgttcgt ttgacgcata ttccaactgg aattgtggta
    24841 tcgtctaccg ttgaccgtac tcaatatggg aaccgcgatc gcgctatgaa gatgttgcag
    24901 gctaagctat atcagttaga acaagaaaag aaagcacaag aagtagatgc tctcaagggt
    24961 gataaaaaag aaatcacttg gggaagtcag attcgttctt atgttttcac cccttatacg
    25021 atggtgaagg atcatcgtac taactttgag cttgctcagg tagataaggt tatggatgga
    25081 gaaatcaatg gctttattga tgcctacctt aagtggcgca ttgaagacta aaaataataa
    25141 aggagaaaca tggcactaat agaaatgtct ggtgttacta aaaaataccg ccgctcaacg
    25201 accgccctca gagacgtcaa tgtttctgtt aatcaaggtg agtttgttta tctcgtaggc
    25261 ccatcaggtg ctggtaaatc aacttttatc aaactccttt accgtgaaga acaactgaca
    25321 actggtaagc tatatgtggg tgaattcaat ttaactaaac taaaagcacg tgatgttccg
    25381 attcttcgtc gtcacatcgg agtagttttt caagattaca aactgctacc aagaaaaaca
    25441 gtctttgaaa atgtagctta cgccatggaa gttattggtg aaaagcgccg ccacattaaa
    25501 aaacgcgtgc cagaggtact cgatttagtt ggcttgaaac ataagatgcg ttcttttcca
    25561 agtcagctat caggagggga gcagcaacgt gtggccattg cgcgtgcgat tgttaacaac
    25621 cctaaattat tgattgcaga tgaaccgaca gggaatttag atcctgagat ttcatgggaa
    25681 attatgcagt tacttgaacg tattaatgtt cagggaacaa ctatcttaat ggcgacacac
    25741 aacagtcata ttgtgaatac atttcgtcat cgtgttgttg ctattgaaga tggtcgcata
    25801 gtacgtgatg aagaaaaagg agattatggt tacgatgatt agatactttt tccgtcatat
    25861 ttgggaatcc attaaaaatc ttaaacgcaa cttttggatg acatttgctt cagttagtat
    25921 ggtggctgtt acattgactt tagttggtgt ttttgcggcg acattgttga atatacagcg
    25981 tgttgcttcg ggtgttgaaa ataatgtcca tatcaatacc tacttacaag ttgattcaac
    26041 tgatgctgca aaagttattc aaaatactgc cggtgaacct gttaataacg ataactacca
    26101 cagtgtttac gacaaaattg ctcagataaa aggtgtcaaa aagattacat tttctagcaa
    26161 ggatgaacaa ttgaaaaaat tgcaagaaac cttaggtgat gtttggaata tgtatgacca
    26221 agacactaat ccgctccaag atatctatct tattgagacc cagacgccta aacaagttaa
    26281 ggcgatcact aaaaagatca gaacgattga aggcgtagaa gccgcagatt atggagggat
    26341 taactccgat aaactattta agttttcaac attaattcaa acttgggggt tgatcggaac
    26401 agcaatgcta ttatttgtag ctgtctttct catttcaaac actattcgta tgactattat
    26461 gtctcgtaaa cgcgacattg aaattatgcg gttagttggt gctaaaaatt cttatattcg
    26521 agggcctttc ttttttgaag gggcttgggt tggcttgtta ggagctgttt taccatctct
    26581 tctgatttat tatggatatg atcttgttta taagcatttt gcacaagaat tacaacgtaa
    26641 taatctctcg atgtatccgc ttgatcctta tgtatattac ctgattggtg ctttgtttgt
    26701 cataggtatt atgattggtt cgcttggttc agtgctttct atgagacgtt atttaaaatt
    26761 ctaaaaaaag cgctgctgac gaaacgtagc gaccctcaaa agttggacct cttatgttat
    26821 tccaaggact tcgttttgta ttgcacagga ctggtccttt tcattttgtt ttgattcatt
    26881 tgtttatttg taataggcaa tatagtttaa aatagcagtt tctaaggttt caagagaaga
    26941 aaagcacccc aaaagttaga tgtttgatgt atctaacttt tggggtgtca ttcaaaattt
    27001 agtggctctt ttttagtaaa atactatgat ttcagtttta ggataacctc aaacagtatc
    27061 taaaatagct atgtcaatga aaaaaggggt tagcattttt ttcgtgacaa atggtagtag
    27121 aaggaccatg cccaggataa actcggtaat gattaggtaa ggtgaatagt tcttggcgaa
    27181 taccagtaat gaggtcctca aagttactgg taggtaagtc agtacgaccg atagtttcac
    27241 gaaatagagc atcgccagta acgactaatt catctgagtg gaaaacaaat gagactcctc
    27301 cccaagaatg accaggtgtt ggtaaaactg tgaattcaaa accatttagt tgatagggtt
    27361 gctttaattt aaaaaagttc tctgccggtc ttgctataac agtaatgata tcatcatgac
    27421 ggcctaaccc agataaatta tcatctgggg aagatagcca agcggcttct ttttctgaaa
    27481 cataaacagg tggatgatca aaagcatctc tgaccaaatc taaactaaaa atatgatcat
    27541 aatgagtatg cgttaagagg atagccacta aaggtttttc aaaggattta attttagcaa
    27601 tgatttcttg gccgttactt cctggatcaa tgaggataac agcttgatca ttaacgaggt
    27661 aataagtatt ttcaccagca atgtggttta ttgttttata gattttcata aggatagtgt
    27721 accaaaaaat caaggctttg ttaaaaaaag agtggcggaa aataaatggc aattagtttc
    27781 agaaaatatg agatctcaca gcaatttctt gacaccgttt tcataaaggt gtatcataaa
    27841 agtaatcaat aagccaagcc taaagctgaa cttagtttaa gaacactgac tttttgagag
    27901 caacctaatc ggttgactaa aaaatatgag gtggaagata tgtcaaaagt tgctattgtt
    27961 actggtgctg gtcaaggaat tggatttgct attgcgaaac gtcttcatgc agacggcttt
    28021 aaaataggca ttttagacta taatgaagag actgctcagg ctgcggttga taaactatca
    28081 cctgaggata ctgttgcagt tgtagcagat gtttcaaaac gtgaccaagt ttttgatgct
    28141 ttccaaaaag ttgtagatac ttttggtgat ttgaatgttg ttgttaacaa tgcaggtgtt
    28201 gccccaacaa cccctcttga cacgattact gaagagcagt ttgaaaaggc ttttgcaatc
    28261 aatgtcggag gaacgatttg gggaagccaa gcagctcaaa aacattttag agaattaggt
    28321 catggcggaa aaattataaa tgcaacgtca caagcaggat gtgaagggaa tcctaatttg
    28381 actgtttacg gtggtacaaa atttgcagtt cgtggtatta cacagacttt ggcaaaggat
    28441 ttggcatcag aaggaatcac agtcaatgct tacgcaccag gtattgttaa aacacctatg
    28501 atgtttgata ttgcccatga agttggtaaa aatgcaggca aagatgatga atggggtatg
    28561 gagcaatttg caaaagatat cactttgaaa cgcttgtctg aaccagaaga tgttgctaat
    28621 gcagtaggct tcttagtggg agatgattca aattatatca ctggacaaac aatagttgtc
    28681 gatggtggta tggtattcca ttaaaaatga ttaatactga cactgtgagt ttcactcaca
    28741 gtgtttttta tatttcttaa aaaattatcc tatcttgatt attggagtga tggcaccttt
    28801 gaggtatagt catgttttgt tttatagata tagcgtgcta taatagactt actatgaccc
    28861 agaaaaaact tagaaaatat gcagtagtgg acttggaggc tactggagca ggtcccaatg
    28921 cttctattat tcaggttggg attgttatta tccaaggaaa taaaattatt gatagttatg
    28981 aaacagatgt taatcctcat gaatctttgg atgagcatat tgttcatttg acaggtatta
    29041 cggataagca gttggctaaa gctccggatt ttggtcaagt ggcgcatcac atttatcaat
    29101 tgattgaaga ttgtattttt gttgcccaca atgtaaagtt cgatgcaaat ttattggcag
    29161 aagctctttt tttggaagga tatgagttaa caataccacg tgttgatact gttgaattag
    29221 cccaactatt tttccctcgc tttgaaaagt ataaccttag tcacctatct aggcagctca
    29281 atattgattt ggcagaagct catactgcta ttgcagatgc aagagctaca gctatactgt
    29341 ttttaagact tttacaaaaa attgagagcc ttccaattga gtgcttagaa agcttattag
    29401 cctattcaga tagcctgcta tttgaaacag caatggtcat tcaagaagga ttagccaagg
    29461 ccaaacctta tgatcccaac aaatacatta aaatcagaca gattctctta ccaaagggct
    29521 caaaagctct caagccttat caaatatcaa aaagcttccc tattaatatg gcactattag
    29581 gtctggaaga aaggcctaaa cagactcaat ttgctcagtt aattgacgaa gattaccatc
    29641 agggagtagc tagttttatt gaagctcaga caggtattgg aaaaacctac ggctacctgc
    29701 tacccctttt ggcaaaagaa gatcaaaacc aaatcattgt tagtgtgcca accaaacttc
    29761 ttcaagacca attaatggct ggtgaagtag ctgctattca agaacaattt cacatcgcct
    29821 gccatagttt aaaagggcct gccaattacc taaagctaga tagctttgca gatagtttgg
    29881 accaaaatga ccagaaccgt ttggtgaatc gttataaaat gcaattattg gtttggctct
    29941 tagaaactaa gacaggagac cttgacgaaa tcaaacaaaa gcagcgcttt gccgcttatt
    30001 ttgagcagct taagcacgat ggagacatta agcagtcttc tgaattttac gattatgatt
    30061 tttggagagt tagttatgaa aaagccaaaa cagcccgtct cctgattacc aaccatgctt
    30121 attttttaca ccgtgttcaa gatgacaagg actttactag aaacaaggtc ttggtatttg
    30181 atgaggccca aaagctgatg ctccaattag atcaattatc acgtcatcag cttaacctga
    30241 cggttttttt acaaacaata caggccaagt taagcaaccc tttacctctt ttagaaaaac
    30301 gcttattgga aagcttgtcg tttgaactgg gacaagtgtc aagtgattac tatcaaaata
    30361 aagagcacca gcttgctcat gattggtctc gtatagctgg ctacgccaaa gaattaacag
    30421 gagcagatta tcaagagtta caagcctttt ttgctacctc agatggtgac tactggctta
    30481 gtagcgaaaa gcaagaggag aagcgagtga cttatctaaa cagtgctagt aaggcattta
    30541 ttcatttcca acagctctta ccagaaacgg tgaaaactta ttttgtttct gccactttaa
    30601 ccatcagctc agaagtaacc ttggcagacc tattaggttt tgaagagtat ctctatcatg
    30661 tcattgaaaa ggataaaaag caagaccagt tggtgcttgt tgatcaagag gcgcctatcg
    30721 tgacagaagt cagtgaccaa atctatgtgg aagcaattgc aaaacgaatc gaaagcttga
    30781 agcaagaggg ttatcctatt ttagtgcttt ttaattctaa aaaacacttg ctgttggttt
    30841 cagactatct agaccaatgg caagtgcctc acttagcgca ggaaaagaat ggaacagctt
    30901 ataatattaa aaaacggttt gatcaaggag agcaaactat tttgcttggt ttaggttctt
    30961 tttgggaagg agttgatttt attcaagcgg atcgtatgat tacccttatc gcacgattgc
    31021 cctttgataa cccagaggat ttctttgtta agaaaatgag tcattatcta ttggaaaaag
    31081 gtaaaaatcc tttcagagat tacttcttgc caatgactat tttgcgatta aaacaggcta
    31141 ttggtcgcac gatgcgtcga caagatcaaa agtcagtggt tattattttg gatcgtcgtc
    31201 tccttacaaa gtcctatgga caagttattt tggaaggtct cggtcaggaa tttttgattt
    31261 ctcaacaaaa ttttcacgat tgtctagtag aaacggactg ttttctgata taatggaaag
    31321 gatctattat atcagaaaaa tttttaatgg cgttaagcac aatcaactaa catttaaatt
    31381 agtttgaact tttattatgc tcttttagac aactgttcaa atgttttcga attgtttttt
    31441 tgataaggac aataaatgaa caaatttttg gttttagtaa acccaattac tgttcttttc
    31501 attaatgtca gttgctatca aaacagcttg tcatagttcg tgcgatcagt agcgagtatt
    31561 ggtaaaactg aaaccgtaga ttatgaaaaa atgatctgtt gatcattaat taatctgaag
    31621 ataagaagtc gataaaaagg agatacgatg cccaaactat caaagcgagt tttagagatg
    31681 aaagagagtg ttactctggc tgcaggtgct agagctaagg ctttgaaagc gcagggtcgt
    31741 gatgtactga atttaacttt aggagaacca gattttttta cacctaagca cattcaagac
    31801 aaggcgattg aatctattca aaatggtacg gctagctttt atacaaatgc ttctgggctt
    31861 ccagaattaa aagctgcgat tgccacatac ctgaaaaatc agtatgggta tcacttgtct
    31921 cctgatcaga ttgtggcagg aacaggtgcc aagttcatcc tttatgcctt ttttatggct
    31981 gttttaaatc ctggtgacca agttctcatt ccaacaccat attgggtatc ttattctgac
    32041 caagtaaaaa tggcagaagg tcagccgatc ttcgttcaag gactagaaga aaatcaattc
    32101 aaggtaactg ttgaccagct ggaaagagca aggacaagta agactaaagt tgtccttatc
    32161 aattctcctt ccaatccaac aggcatgatt tatggtgctg aggaattaag ggccattgga
    32221 gaatgggcgg tgcacaacga tattcttatt ctggcagatg atatttacgg gtccttagtc
    32281 tataacggca atcagtttgt tcctatttca acactctcag aagcgattcg tcgtcaaacc
    32341 attacggtca atggcgtggc taaaagttat gccatgacag gctggcgggt tggttttgca
    32401 gcaggtgaac cagagatcat ttcggcaatg agtaagatca tcgggcagac tacctctaac
    32461 ctgacaaccg tttcacaata tgctgctatc gaggctttct gtggtagtca gtcatcactt
    32521 gaagagatgc gattagcatt tgaagagcgc cttaatatca cttatccatt gttgtgtcag
    32581 gtccctgggt ttgaggttgt taaaccacaa ggagcttttt acttctttcc aaacgttaag
    32641 aaagccatgg aaatgacagg tttttccgat gtgacaagct ttgctaatgc tattttagag
    32701 gaagtcggat tagctgttgt gagtggagca ggctttggcg caccggaaaa tgttcgtctg
    32761 agttatgcta cagatatcga aacccttaaa gaagctgttc gtagactgca tgtctttatg
    32821 gaaagtaatg aaattaatta aattaattaa ataaaaatag agagaaactt atgtcaaaaa
    32881 aacttatttc aattgttgat gttaaagatt atgttggtca agaagttact attggtgctt
    32941 gggttgctaa caaatcaggt aaaggtaaga ttgcgtttgt acagttgcgc gatggttcgg
    33001 cttttttcca aggtgtcgct tttaaaccaa acttcattga aaaatatggt gaagaatcag
    33061 gtcttgaaaa atttgacgtt attaaacgtc tcaaccaaga aacatctgtt tatgtaacag
    33121 ggattgttaa agaggatgag cgttctaaat ttggctatga gcttgatatt actgaccttg
    33181 aaatcattgg agagtcacat gaatatccta taactcctaa agagcatggg acagacttct
    33241 taatggacaa ccgtcatctt tggttgcgtt ctcgtaaaca aatggctgtt atgcaaattc
    33301 gtaacgctat tatttatgca acttatgaat tctttgacca aaatggtttt atcaaatttg
    33361 atagcccaat tttatcagaa aatgcagcag aagattcaac agagttattc gaaacagact
    33421 attttgggaa accagcattt cttagccaat ctggtcaatt ataccttgaa gctggagcaa
    33481 tggctctagg ccgagttttc gactttggtc cagtctttcg tgctgaaaaa tctaaaacac
    33541 gtcgccattt aacagaattt tggatgatgg atgctgagta ttctttctta tcacatgagg
    33601 aatcacttga ccttcaagaa gcttatgtta aagcattgat ccaaggcgtt ctagatcgtg
    33661 cgcctcaagc acttgatatt ttagaacgtg atgttgaggc tctcaaacgt tatatcacag
    33721 aaccattcaa gcgtgtttct tatgatgatg ctattacact tcttcaagag catgaagcag
    33781 atgaagatac agattatgaa catttagagc atggtgatga ctttggttcc ccacatgaaa
    33841 cttggatttc taattacttt ggagtaccga catttgtagt gaattaccca gcaagcttta
    33901 aagcatttta tatgaaacca gttcctggaa atccagaacg cgttctttgt gctgatcttt
    33961 tagctccaga aggttatgga gagatcattg gcggctcaat gcgtgaagac aactatgatg
    34021 ctcttgtggc taaaatggat gaactaggta tggataaatc agaatatgac ttttaccttg
    34081 accttcgtaa atatggctct gttcctcatg gcggttttgg tataggtatt gaacgtatgg
    34141 taacttttgt tgctggtacc aaacatattc gcgaagctat tccattccca cgtatgttac
    34201 atcggattag accctaataa tacaaaaaag ttcatgtcat ctatgaactt tttgtattgg
    34261 taagaggtga gtgtgttata aaaaaatcaa tatgtcctaa aggagagata agaaatagta
    34321 gagtttgaac ctacatcctt tgtgaagata aattttggaa gtttagtaga tcagttagca
    34381 gataagacaa tatagccttg ctcaagggat attttataga caagtatttg gactacgatt
    34441 atcaaataga cagtctcatt tgatactaaa aaggtctcgt ctattcatat aataggagag
    34501 ttacgggagt tattattaaa attagctgat ttttacagtt caaaatgtgt taccactttt
    34561 tcacttctta gctttcttat gtatgtggta taatgaaggt ggacggttcg atagagaaag
    34621 ggaaattgta aatgtcagac aaacacatta atttagttat tgtgacagga atgagcggcg
    34681 ctggaaaaac agttgccatt cagtcttttg aggatctagg ctactttacc attgataata
    34741 tgcccccagc cttggttcca aaatttttag aattaattga acaaaccaat gaaaatcgta
    34801 gggtggcttt ggttgtcgat atgagaagtc gtttgttttt caaggaaatt aattctacct
    34861 tagatagtat tgaaagcaat cctagcattg attttcggat tctttttttg gatgcaacgg
    34921 atggagaatt ggtgtcacgc tataaagaaa ccagacggag ccaccctttg gctgcggacg
    34981 gtcgtgtgct tgatggtatt cgattggaaa gagaactcct atctcctttg aaaagcatga
    35041 gccaacatgt ggtggataca acaaaattga cccctagaca attgcgtaaa accatttcag
    35101 accagttttc tgaagggtct aatcaagcct ctttccgtat tgaagtgatg agctttgggt
    35161 tcaaatatgg tcttcctttg gatgcggatt tggtttttga tgtgcgtttt ctacccaatc
    35221 cttattataa ggtagagctt cgtgaaaaaa caggactaga tgaggacgtt tttaattatg
    35281 tgatgtctca cccagaatca gaggtgtttt acaagcattt gttaaacctt attgtcccta
    35341 tcttaccggc ttaccaaaaa gaagggaagt ctgtcttgac ggtggctatt ggctgcacag
    35401 gaggccaaca ccgcagcgtt gcctttgccc attgcttggc agaaagtctg gcaacagatt
    35461 ggtcggttaa tgaaagccat cgtgatcaaa atcgtcgtaa ggaaacggtg aatcgttcat
    35521 gaaaaatcct aaaatgactg ttataggtgg gggtactggc atctccatta tcctaaaaag
    35581 ccttcgtaat gaagcagttg atattactgc tgtggtcact gttgcagatg atggtggttc
    35641 ctcaggagaa cttcgtaatg ccatgcaatt aaccccgcca ggagacttac ggaatgtctt
    35701 gttagcgatg agtgacatgc caaaatttta tgagcgggtt ttccaatacc gttttaacga
    35761 gagtgatgga gccttggctg gtcaccctct aggcaatctc attattgctg gtatctctga
    35821 aatgcaaggc tcaacctaca atgccattca aattttaacc aaatttttcc atattacagg
    35881 taaaatttac ccctctagcg agcaggcctt gacccttcat gctgttttta aagatgggca
    35941 cgaagtagca ggagaaagtt ctattgccaa atatcaaggc atgattgacc atgtctatgt
    36001 gaccaacacc tacaacgacc aaaagcctca ggccagtcgt aaggtggttg aggctattct
    36061 agaaagtgac atgattgtct tgggaccagg ctcactgttt acctctattt tacctaattt
    36121 ggtcattcca gaaattaaag aagctttgcg ccagactaaa gcagaagtgg tgtatatttg
    36181 taatatcatg acccagtatg gtgaaacaga acaattttca gatgcagacc atgtggctgt
    36241 tttaaatcag cacttaggaa gagatttgat tgatacggtt ttggtaaatg tggctaaggt
    36301 gccgcaggct tacatgaatt ctaacaaatt tgatgaatac ctcgttcagg ttgatcacga
    36361 ctttgctggc ctttgcaggg ctgctaagcg tgtgatttcg tcttattttt tacggttgga
    36421 gaatggcgga gcttttcacg atggtaactt agtggtggaa gaattaatga atttagtgag
    36481 gatagtcaag caatgagttt tacaaccaag gtcaaagagg aattaattca cttgtccaca
    36541 ggtgacaaca atgaattggc cgccattatc aaattatcag gcagcttggg cttagctcac
    36601 caaagccttc atttatccat taccactgaa aatgctaaaa ttgcccgcta catttattct
    36661 cttattgaag acgcctatgt tatcgtgcca gaaatcaggt atcatcaaaa gacgaacctg
    36721 cgcaaaaacc gtgtttacac cgtctatgtt gagcaaggtg tggaaaccat tttagccgat
    36781 ttgaaattag cggactcctt ttttggtctg gaaacaggca tcgaacctca ggttttatca
    36841 gacgataatg ctggccgttc ttatcttaaa ggggcttttc tagcggcagg tagcatcaga
    36901 gatcctgagt caggcaagta tcagttagaa atctattcag tttatcttga tcatgctcaa
    36961 gatttagctc aactcatgca aaaatttatg ctagatgcca aaaccatcga gcataaaagc
    37021 ggtgcagtta cttatgtgca aaaggcagaa gatatcatgg actttttaat tataattggt
    37081 gccatgtcct gcaaagaaga ctttgaagcc atcaaattac ttcgagaagc tcgaaatgat
    37141 attaaccgtg ccaataatgc agaaacagct aatattgcca aaactatcag tgctagcatg
    37201 aaaaccatta ataatattat taaaatcatg gacacaattg gtttagaaag tttaccgatt
    37261 gaattgcaac aggtagccca gcttcgggtt aagcatccag actattccat tcaacaagtt
    37321 gctgatgctc ttgagttccc aatcaccaag agcggggtca accatcgctt gcgtaaaatc
    37381 aataaaattg cagatgactt atagaacatt tttatcgtat gagcctttgt tcatacgatt
    37441 tttttgtcta aaagtcgttt tccattgttt ttttgaagtg tctgatgttc tgtgatatga
    37501 taaaagggat aataacgttt gtaaaggaga ctgataatgg catgtacaac tatactcgtc
    37561 ggtaaaaagg catcttatga tggctcaacc atggttgctc gaacagaaga ttctcaaaat
    37621 ggtgatttca cgcctaaaaa aatgattgtg gtgaaaccag aagatcaacc gagacattac
    37681 cgttcggtgc aatcttcctt tgaaatggac ttaccggaca atcccatgac ttacacgtct
    37741 gttcccgatg cgcttggtaa agatggtatc tgggcagaag caggcgtgaa cgaggctaat
    37801 gtggccatgt cagcaactga aaccatcact accaattctc gtgttttagg ggcagatcct
    37861 ctagtagctt ctggtattgg tgaggaggac atggtgacct tagtgctccc ttatatccgt
    37921 tctgcgcgtg aaggggtttt gcgccttggt gccattttag aagactatgg cacttacgag
    37981 tcaaatggtg tggctttttc agatgaacat gacatttggt ggctggaaac gattggtggt
    38041 catcactgga ttgcaagacg ggtgccagat gatgcttatg tcaccaatcc caatcaattt
    38101 ggcattgatt attttgaatt taacaatcct gaagattacc tttgctcagc tgatttaaag
    38161 gactttattg acacgtatca cttagattta acctataacc atgagcattt taatccccgt
    38221 tatgcctttg gtagtcaacg tgacaaagac cgtcactata atacgccacg tgcttggatc
    38281 atgcaaaaat tcttaaaccc agaaattgtg caagacccgc gcagctttgc ccttgcttgg
    38341 tgtcaaaaac cttaccgtaa aataacagta gaagatgtca aatatgtatt gagtagtcac
    38401 tatcaggaca cggtttatga cccttacggt tcagaaggaa ctcctgttag taagaaagtc
    38461 tttcgcccta ttggcattaa ccgtaccagc caaaccgcta tcttacacat tcgtcctaat
    38521 aaacctcaag aaattgcggc tatccagtgg atggcttatg ggtcaatgcc atttaatacc
    38581 atggtgcctt ttttcacaca ggtgaagact attccagatt attttgccaa cacttacgaa
    38641 aatgtgtcta ccgacaattt ctattggacc aaccgtctga ttgcggcttt ggcagatcct
    38701 cactacaatc atcatgaaac tgatttggac aattacttgg aagaaaccat ggcaaaaggt
    38761 catgccatgt tacatgctgt ggaagcacag ttattggctg gtgaaacagt cgatttggaa
    38821 gaggaaaatc aaaaaatgag tgactatgtg caaggagaaa cgcagacgct gttgaacaag
    38881 attctctttg acgctagcaa tctaatgacc aatcgtttca gtttgagcga ttaaaagtga
    38941 agctaaggct gagcaactgc ttagcctttt ttacgataac tttttaggaa aagtaatcgc
    39001 aaaaagacaa aaacttggaa aaaaaatcaa agtatgctat acttaactgg tcaattttta
    39061 accagttaat aacaaaggag gaatcatgaa aaagaaaatt cttttaatga tgagtttaat
    39121 cagtgtcttt tttgcttggc aacttactca ggcaaaacaa gtcttagcag agggtaaagt
    39181 gaaggtggtg acaactttct atcctgttta tgaatttaca aaaggggtta ttggtaatga
    39241 tggcgatgtt tccatgctta tgaaagcagg aacggaacct catgattttg agccttctac
    39301 aaaagacatt aaaaaaatcc aagatgcaga tgtatttgtt tatatggatg acaatatgga
    39361 aacttgggtt tctgatgtga aaaaatcatt gacatctaaa aaagtgacca tcgtcaaggg
    39421 aactggtaac atgctcttgg tagcaggagt tggacatgac catcaccatg aggaagctga
    39481 caaaaagcat gagcataata aacatagcga agaaggacac aaccatgctt ttgacccaca
    39541 cgtgtggttg tcaccatacc gtagcattac ggtcgttgaa aatattcgcg acagtctttc
    39601 aaaagcttac ccagaaaaag cagagaactt caaagccaat gccgctactt atattgaaaa
    39661 attaaaagag cttgacaaag actatacggc agcactttca gatgctaagc aaaagagctt
    39721 tgtgacacaa cacgcagctt ttggttatat ggcacttgac tatggcttga accaaatttc
    39781 tattaatggt gtcacaccag atgcagaacc atcagcaaaa cgtattgcta ctttgtcaaa
    39841 atacgttaaa aaatatggta tcaaatacat ttattttgag gaaaatgcgt caagtaaagt
    39901 cgcaaaaacc ctagctaaag aagcaggagt taaagcggct gtgcttagtc cgcttgaagg
    39961 tttgactgaa aaagagatga aagctggcca agattacttt acggtcatgc gtaaaaacct
    40021 tgaaacctta cgcttaacca ctgatgtggc tggtaaagaa attcttccag aaaaagacac
    40081 gactaagaca gtttacaatg gttatttcaa agacaaagaa gtcaaagatc gtcaattatc
    40141 tgactggtca ggtagctggc aatctgttta cccctatcta caagatggta ctttagacca
    40201 agtttgggac tataaggcta aaaaatctaa aggtaaaatg acagcagccg agtacaaaga
    40261 ttactacact actggttata aaactgacgt ggaacaaatc aaaatcaatg gtaagaaaaa
    40321 gaccatgacc tttgttcgta atggtgaaaa gaaaaccttc acttacacat acgccggcaa
    40381 agaaatcttg acctatccaa aaggaaatcg cggagttcgt ttcatgtttg aagctaaaga
    40441 accaaatgct ggcgaattca aatacgttca attcagtgac catgccattg ctcctgaaaa
    40501 agcagagcat ttccacctgt actggggtgg tgacagccaa gaaaaattac ataaagagtt
    40561 agaacattgg ccaacttact acggttcaga cttatctggt cgtgaaattg cccaagaaat
    40621 caatgctcat taatcttaac tcactaagca aatcttcttt taaggaggtt tgctttttct
    40681 ttgtggagta aaaaaattta ataattctca acattaattt gacggacact tatgattata
    40741 atatacttta agtggttata acaagttaga ggtgaataag atgccaaaag aacaaccact
    40801 ctatctacag attgttgatg acttggaagt gaaaatcaaa aagagcatga cagaaaacga
    40861 taagttgttg tcagaacgag aattaagtga cctttacggt gttagtcgta tcacgattag
    40921 actggctctt aaggaattgg aattacgaga cttaatttat aaaaagcagg gcaaggggac
    40981 ttatgtttca ggaatcaaag aacctgtcac tgatttatca agtacttaca gctttacaga
    41041 gcaaatgaaa aaagtgggga aaactcctaa aacagagatt ctttcttttg aacagtatca
    41101 ggtaacacct tacttgtctg gtctcttaga gtttgatctc gatacagaag tttttgaatt
    41161 agaacgccta aggattgcag atgatatgcc cttgatgttt gaacgatcct atattcctgc
    41221 gcagcctttt caggggttaa gcattgcgga cttgaagcgt aaagctttgt acgatatctt
    41281 tgcaaaggac tatcaagaga caatccgtct tgcagaagaa gagttttatg ccagcattgc
    41341 cttggactat gaagcagacc ttttgggaat caaaaaagga gatcctgtct tacatattat
    41401 tcgtaaaact tataacgata aaaatatttt gatcgaattt actttcagta ttgcaagagc
    41461 agaccagttt aggtataggg tacaacatca ccccaatggt taaagaaatg ggaggttaat
    41521 acatgtttac aaaaacgcag gaagctttgg aggctcttgg tgcagctatc actaccaagg
    41581 aaattaaaca agaaccccgt ttatggcaag aaaccatgac gttttttgaa gaaacaagag
    41641 attctctgga ttctttttta aagagagttt gtaaaagcgc taatggaaat aacgttcatg
    41701 tcatctttac aggtgcgggt acatctgagt acatcggtaa taccatttgc ccttacttga
    41761 agaaggtggg gaatcggcag tgctatctct ttgagagtgt tgcttcaacc gatttggttg
    41821 cggcgccaga ttattatctt gctgaggaag agacggtact attagtctcc tttgctagga
    41881 gtggtaatag tccagaaagt gtagcggctg ttaacctggt caatcaacta gtgccaaata
    41941 gttatcattt aaccattacg tgtgccaaag atggagaact ggcgaaaaaa gcacagcaag
    42001 atgagcgttc ttacctttat ttgatgcctg aagacgctaa tgatgctggt tttgccatga
    42061 ctggtagttt tacctgtatg atgctagcgg ctcttttgat ttttgatgat gctaacagta
    42121 tagctcagaa agaagcttat gtgactgata tgattcttgc tgggcaagct gttattgacc
    42181 aagaaagacg gctacaagag ctagctgatt taggatttga acgtttagtt tatctaggtt
    42241 ctgctggttt agccaagtta acccaagaag ctcaattaaa aatgttagaa ttgacagctg
    42301 gacaagttgc gaccctctac gagtcctcta tgggctttag gcatggtccg aaatctttta
    42361 ttaatgaccg taccttagtc attggatttg tcaataatga tgcttacgtc agacagtacg
    42421 atttggacat gttagaggaa atccaagcag atggcattgc ccttaaaatc ttggctctat
    42481 tgcaaggagg ggatatcaac ttttcagagg accaatttcg actagacacc cgtcacttat
    42541 tgccagaagc ttacttagct tttcccatga ttttagtcgc gcagaccttg gccctcttaa
    42601 cggccgttac attaggaaat tcccctgaca ccccatctgc gacaggaacg gttaatcgtg
    42661 tggtcaaagg agtaacgatt cacccttacc cgactagtag tcgtcaatgc aacaaagctt
    42721 aaagcttttg tgtagaagcc gtaaaacgaa atgattttgt gctagtaggc caaaagaaaa
    42781 accctcctga tacaggaagg ttttttgtat tgtcttgctt attttgctgc gtttgcgtct
    42841 ttgagaccgt attttttgtt gaagcggtcc acacgtccat ctgcttgagt gaacttttga
    42901 cgtcctgtgt agaatgggtg tgaatctgat gaaatttcca cacggataag tgggtaagtt
    42961 tcaccttcaa actcaacagt ttctttagaa gcttttgttg agccgctaag gaactggtaa
    43021 cctgtagttg tatcaaggaa aacaactgga cgataatctg gatgaatgtc ttttctcatc
    43081 ttaaaaaatt cctttctgcc atgacacttg atgcatcata gttattaact attctagtgt
    43141 aacaaaactt gaatgacttg acaaggcttt tctttcctta ttttttaaaa atcctaaatc
    43201 tcttggcaaa cagcgatgag ttctcggaaa atagcttgat tttcttctaa attagctgag
    43261 ttggctccgc tagcaagggg atgcccccct ccaccgtgac gcttagcaat gccattaata
    43321 atcttttctt tactgcgcat acgcacacgg tagttgccgt cagttaactc aacaaaaata
    43381 gcccaagctt gaacgttatc aattttacca ggagcacaga caatggcaga gctttctgct
    43441 agggtcacgt caaaatgttt taaggtttct tggctgacaa ggacataagc agccccactc
    43501 tcatcaattg ttaaatgctc aaagacgtag ctttgcagct ttgctatttt caaaggaaac
    43561 gaatccattt gccttgaaat cgcagcaaag tcgaactcga aatgtctgag ttggctagca
    43621 atggaaaggg ttttactagt ggttgaggca taaagaaatc gccctgtatc accaacgata
    43681 ccagtgtata agagcttagc agccttgtca gagagagtaa gattctgact aaaggcaaag
    43741 tctgcaatga tttcacttgc gctagaagcg cttgtgtcca cataatagaa gtcaccatat
    43801 acatcatcgt tgggatggtg atcaatctta attaagcact gccccagtgt gtagcgctca
    43861 tcatcaatcc ttggtcgatt cgctgtatct gtaatgatga ccaaagcctc tttatagtct
    43921 ttatcagtca cctgatccat ttggctaatc caagttaaac taggctcatc aaaaccagtc
    43981 atcaaaacct ttttatctgg gaaattttgt gcaataattt ctttcaagcc ggcctgacta
    44041 ccaagagcat cagggtcagg attttgatgg cgatggataa taatagtttg gtgagctttt
    44101 attttatcta aaattgtttc aaaagttgtt atcatgcttt aatctttctt tttcaaaaag
    44161 aggaaaataa ccgtttaaca taatcttcaa aaaacggttt gtgtcataag gaggtgtgcg
    44221 ttatgttaag tggctaatgc tagaattttt taagattgtc aaagctcata aattgacatt
    44281 ttatctgaaa atcatatcct attttattat agcataaaag taagctctag ataatggtaa
    44341 attaatctag gaagaaaaga ggttagttat cagttatgat actatcaaag ctagttttgc
    44401 aaatgcaagt gaaaaaatag tttttaagtg tcttaagtaa tcatatcaac tttctcttta
    44461 aacaattagt gcttttccta aaaaagtgtg ctaaaataaa catagtttag taattttggg
    44521 aggaatttat ggcattagca aaaatcgttt atgccagcat gacgggaaat actgaagaaa
    44581 tcgctgatat cgttgcaaat aaattgcaag aattgggcca tgatgttgac attgatgagt
    44641 gcacaacagt tgacgcttca gagtttgaga atgctgatat cgctgtcgtt gcgacttata
    44701 cttatggtga cggtgactta ccagatgaaa tcgttgattt ttacgaagat ttacaagatt
    44761 tagatttaga aggtaaaatc tatggcgttg tcggctctgg tgacactttt tatgactatt
    44821 tttgcaaatc tgttgatgac ttttcagatc aatttgcgct tactggtgcc gtcaaaggtg
    44881 cagagccagt caaagtagat ttggcagcag aagatgaaga catcgatcgt ttagaggcct
    44941 ttgcagagca actctcacag gctgttaata atctataaaa ctatttagta aaaatgtcag
    45001 ggacttcctt ggcattttta gatttttaac ctaaaataaa gggaatgtca cttcagggta
    45061 ggaattgaaa aaacaatcag cttcaccttg cttctgatcc tcttattaac tataataaaa
    45121 agataatcta atacatctag aaaaggaaag ttatgcgttt agaagagata cgacaagaaa
    45181 tcaatggtat tgatcaccac ttggttgcct tattagaaaa gcggatggca cttgtagagc
    45241 aagtaacagc ttataaacta gccaatcact tgcctgtctt agatcaggcg agggaaaatc
    45301 agatcctaga tagggtttct tatctcgtta aagaccaagc atttgagcca gctatccatg
    45361 agacttttaa aaccattatg tctctttcac ggcagtatca aacgcaacgc ttaacaggtg
    45421 gtgacattaa tgattaaatc aattgcaaca ctgcctaaac cctccttaaa agaagcgtta
    45481 ggactaatag gcttggcgct tcccataggg gccacggttg gagtggttga tgtgattttt
    45541 ggtaaagggc ttttgtttct ttccgaatat cgcgatcacc acctgttctt gcttcttcct
    45601 tttcttgccc tagcaggtct tgttatcgtt tttctttacg ataagttagg caaagaggtt
    45661 cgtcaaggga tgggattggt ttttcaggta ggacatggtc aaaaagatca gattcctcct
    45721 attctcattc ctcttatttt gttctcgact tgggtaaccc acttatttgg agcgagtgcg
    45781 ggtcgagaag gggtagccgt tcaaatagga gctaccatct ctcattattg tcagagattt
    45841 gtgactagtc aagaagccgc acgacacctt ttgattatgg ggatggcagc cggttttgca
    45901 ggactttttc aaacgccgat tgctgctgtc gtatttgccc ttgaggtttt gttggtagga
    45961 acgcttcgct actgcgcttt attgccttcc ttagttgctg cttgcgtggc ttcttggacc
    46021 tctcattctt tgggattaga gaaatttacg attgttcttg aagaaacatt gaccataact
    46081 cccttaacat tggttaaatt aattgggtta ggccttattt ttggtctggt gggaaatagt
    46141 tttgcctatt tacttggctg gtttaaacct tacctcagtc aaaagctacc aaatccttat
    46201 ttccggattg cttttatcgg tgctttgtta tctatctgtc tgatgattgg tcacgtcgga
    46261 agatatagtg gtctaggaac tcatttgatt gcggcagctt tttctggcca aactatccta
    46321 acttacgact ggcttttaaa aatgatcgtg acggtgatta gcttatctgc aggctttcaa
    46381 ggtggtgaag tgacgcctct atttgctatt ggagcttctt taggcatcgt tttggcccct
    46441 tacttaggtc ttcctgtact gttggttgct gcacttggtt acaccacggt atttggaagt
    46501 gcaacaaata ccttttgggc ccctatcttt attgggatag aggtctttgg tccagagaat
    46561 gccctagcat attttgtcac ctcggctgca gcctatatgg ttagtcacag acattccatt
    46621 tacagttatc aaaaagtagt catgatcaat ttaaaagaca tgacggtaaa tcagatggat
    46681 ttaaaaaaat agctttacaa taaagaagct ttttgttaga ataaatctcg tgtgtaatgg
    46741 acacacgaaa attacggcta atccgctaag acaagtactt aagattagta agataggaga
    46801 ataaaaatga atccattaat ccaaagtttg acagaaggtc aacttcgctc tgatatccct
    46861 aacttccgtc ctggtgacac tgttcgcgtt cacgcaaaag ttgtcgaagg aactcgcgaa
    46921 cgtatccaga tctttgaagg tgttgttatt tcacgtaaag gtcaagggat ctcagaaatg
    46981 tacactgttc gtaaaatctc aggtggtatc ggggtagagc gtacattccc aatccacaca
    47041 ccacgtgttg acaaaatcga agttatccgc cacggtaaag tccgtcgtgc gaaactttac
    47101 tacttgcgtg cattacaagg taaagctgct cgtatcaaag aaattcgtcg ttagtttgaa
    47161 ccgaaacgta acgtaataga ataaaaagac tagagtctct agtcttttta tgtcgtcgtg
    47221 acaatgtgac aattggtgat taacgctatg ctcgtaggac actttgaaag ttccttagca
    47281 gttataatta gtatattgta ataacaaaat gaagcaaaat aaactttcag ttatctgata
    47341 tttcatcttg cattacctta ttttctaggt taaaatagaa gacaaggtga atataagata
    47401 ggccccttag ttcaatggat ataacaactc cctcctaagg agtagttgct ggttcgattc
    47461 cggcaggggt cattattgtt aacttaaaaa gcctttatat caggcttttt ttattactaa
    47521 attattttta ggggcataaa aggggcagtt tgtttattta tcaatgcttt catatttaca
    47581 gttgtatgac tgtaaattga taatgttgtg ttggggtctg aatggccaac tctgtccata
    47641 atcgctttta gtggtattcc tttttcggct aaaaacgaaa tgtgtgagtg tctgaataag
    47701 tgagtatgat agttaccata aattttcagg cgcttattga tgtatgcgtt taaaattggt
    47761 aaaccttttg aatttggaaa aataaattca ctcactttat tttgtcgatt gatgatttct
    47821 aaaatgttat cagatacgga aattttgcga gttgattttt tagtcttagt agtcgtaatt
    47881 tctttagtgt aaaaatcgta agttgcgtta atcagaattt ctttattttc aaaatcaatc
    47941 ttatcataag tcagacaagc aagctcacca tatctcatac ccgttaaaaa catgaataaa
    48001 acgacatctg cgagcgtttg ttcattgttg gctatcattc taccacagag gtcataaact
    48061 tcattagatg ttaaataaag taccttttca ggcatatatt cctcttttgg cttaggtact
    48121 aaaacattat ctgtcggatt gcttgttaaa tagtccattt ggattgcgta agacaaaata
    48181 gcgtgtaatc tttttctgca tttatgtgta acatggtacg aatggttttt caataagttg
    48241 tcaataaatt gtctaatgtc gcgtttagtc aacttgctga taattgtatc atctggcaac
    48301 acggttgcga tatgaccgtc tgagactaaa taaccacgct ttgtagagtc tttgaccgtt
    48361 ggtatccatt gatttaaata ttcgcgcttt aattcaccat aggtcatctc agaatgatcg
    48421 cgaatagcga gtctatcttc aatcttttct ttgataataa tacttgcttt attcttagct
    48481 tgattactat tcttatcaag tgtcacagat acttttttat attttccggt taatggatct
    48541 gtataacgtt cgataaattt aaattttcca tttaataatt cttcaatcca catttgtttt
    48601 atacctcaat ttctgttaaa atgggtataa gaaaacgagc catttaatgc ttgtttctta
    48661 tacggtttgg cctcacactc aacgtttggc gatggcgagt gtggggcttt tttatttgtc
    48721 taaaagtaag tagccgtcta tataaattag agggacacta ttctcggaac cacttgttgt
    48781 cgtatattca taagataata aatatcttcc attgatagtg atattatcag tttctaaaag
    48841 cttagtttct agtcgcttgg tttcgataaa caacatatac aagtccccat taggggtgtt
    48901 tgctagaatt ttagtatatt tatctttatc agagatttgt attattttta aattatcgat
    48961 tctaacttta agggaaaagt actttttagg gtctcttaaa attgttttgt agtcataaaa
    49021 tagatactcc tctgatttgt agttatctgt tgttactttg tcaatcttag atattagagt
    49081 gtcaaagtat tctttttcgc tcatatctcc agatacttta ctagtatccg atgatagagt
    49141 ttttgatgtt gtatcttttt ttgagccacc agagcaagct ccgagacata ctattaagac
    49201 tgataaggta aacaaatata ctattttttt cacaataaat cccctagatt aattttttga
    49261 attcgtcttg tattatctct tgcccccacg tcgttgatat ttgatatgtc gtggcaaact
    49321 gcaaccaatt aaaatcaaca acatcatgag atgacaagta attctttatc aattccctaa
    49381 tcataaatct atcggcttgg ttttcgtatt ttagtagcaa tctttgatac tgtttagggt
    49441 catgttcaag atgacctaac tcgtgtaaaa taactttttc tttttctatt tgtgatagat
    49501 tttgattaat gtagattatc cgcttgtctg gatagtaaaa cccttttctg tcccacatcg
    49561 cttcagggaa ctcgaataac ttgatattgt actgactaag tatctcattt atcttcacca
    49621 ttccccactc ctaaagacaa tttaataata gcttcgattt tttctacgtc tgtgtcagat
    49681 aaaggttttc cgtcaaacat cacaacacgt tcacggagat tggataggtc aatagtttgt
    49741 gtcggagagt ttttaggtgt caccccaatg atatgttctg gtgtcgtccc taaagccttc
    49801 gcaaataagt tggctctatc tagcggaaat tttcttgctt tattaaagta taatgataca
    49861 cctgattttg acatgccgac ttttctagat aattcactta tcgagagatt tttctctttt
    49921 ctgatattat ccatcaagtc aattatttca tcgtttgttc gcattttttc gcctttcttt
    49981 tattgactat ctaaattata ccaccgttct caaaaaagca caatcgttct cacggaaaaa
    50041 cttttttatt ttttttagat aaaagtgttg acaataaaga acgatattga tatacttaga
    50101 ttgttctttt aagagaacga aataaaaaga caaaacagaa gagggggaaa tatggcagta
    50161 gattatttac gtgttaaagc ggagcgtatc gccaaaggct acacacaaga ctacatggct
    50221 aagcaactag gatggtctga ccgagctaga tacgcaaaac gcgagaacgg attcgtatct
    50281 tttgatgctg atgagttagc aaaagtagct gaaattttag gcatttcaaa agatgatatt
    50341 ggaatttttt ttacatacaa cgttcactaa aaagaacgat ttgttggttt tagccgcaaa
    50401 aaaaaaacga ctgcgggaac agtcgctaac taaattaatt tacttaatta taccatgaaa
    50461 ggaaacaata tgctagcgaa attaaaaagc ggtattgaag taccttacga agaattatgg
    50521 atggatgata atgatttagc tgaatttatt ggaaagtcat ttgatcaaac gcagcgatta
    50581 ctaagaaaga tgtacaaaga cagaaattac cgtaagtaca ttgacaaggt tggtggtcgt
    50641 tcgacaaaag ttaaaaaatt tgaagaatgg agaaaaacac aaaatgaaaa gcttatttaa
    50701 ctttattttc gcaaaaccaa aaaaacaaga agaaaaacca aaatggacga ttgaaaacaa
    50761 cggttgggaa gccaatgcgc gtagatataa ccagttgcac ggtttgcctg gcaagcagat
    50821 ttggtaggag gaaagaatgg aacaacaact aattaatgat aacgaatttt tgcgagacga
    50881 aaatcgcagg ttaaataatg aattggcgga acattatttt gtagttacag ctaaagccaa
    50941 tttattagat gtcattattg ccagtggttt tatcttgcaa agtacgatag ataaatgtat
    51001 agccgaattg gatgaaattg atcaaatgga acttagaaag gtatggaata aatagtatga
    51061 gattagcaaa gcaaaaaatt ggagctcaac aaaatggata tggccgcatt ggatgtgata
    51121 ttgaaattta taatgaaatt gctgaaattg cagataaatg tggatatacc ctgacgagct
    51181 taacaaatgc tcttttaggt tatgctttag agaatagcga gctcacagca gaaacaaaaa
    51241 cagttgaagt ttttaaattt actataggaa acgaggattt cgataatggt aacaattaat
    51301 aaattggaaa ttgaaaacgt taagcgtatt aaagcggtaa agattgagcc gtcggcaact
    51361 ggcttgacta ttgttggtgg aaacaataac caagggaaaa caagcgttct agacgcaatt
    51421 gcttgggcac taggcggcaa taaatacaaa cctagccaag caacgcgaga agggtctatg
    51481 gtgccgccta cgcttaaaat caccatgtct aatggactta ttgttgaacg gaaaggtaaa
    51541 aacgccagcc ttaaagtcat tgatccaaac ggtcaaaaag gcggacaaca actactagat
    51601 agctttgttg aagagttagc tattaactta ccaaagttta tggatggaac accaaaagag
    51661 aaagcagatg tcttgcttga aattattggt gtcggtgatc aattagctga attagaattc
    51721 aaagaaaaag aaatctataa tcaacgtcat gctatcggtg ttatagcgga ccaaaaagaa
    51781 aagtttgcta aggagatgac atattatcct gacgcaccta aacaactggt aagtatttct
    51841 gagcttatcc aacaacaaca agctatctta gccaagaacg gagagaatgc tcaaaagcgt
    51901 caaaatgtag aacgtatccg ctatgattat gatcaatcta tcttggaagt tgaccgctta
    51961 cgcaagttat tagcagatgc agaagctaag acgaataagt taagcgagga tttaaaaata
    52021 gcaaatactg acgctatgga tttacatgat gaatcaacag cagaaatcga ggcaaatatc
    52081 gccgatatcg acgaagtcaa ccgtaaagtt cgcgctaact ttgataaaga taaggcagag
    52141 gaagacgcta agcagcaacg tgatcagtac aacacattga ctaatgagat tgaggttatt
    52201 cgccagcaga aacgagattt attgactaat gcagacttgc cacttgaggg gcttaccgtt
    52261 gatgatggca aactgctgta tcttggccag gagtgggaca acatgtcagg aagtcaacaa
    52321 cttatggtag ccactgccat tgtacgaaaa cttaaaccag agtgtgactt cgttttgatt
    52381 gataaactcg aacaaatgga taacatcaca cttacacagt ttggccaatg gctagagcaa
    52441 gaaggattgc aagctatcgc aaccagagtt tcaaccggtg aagagtgcgc gataattatc
    52501 gaggacggct actccgtgga aaataaggct cataattttg aaactgcagc agcaggtggt
    52561 tttgccgaaa cagtagcacc cgcttggcaa ggtggatttt agaaaggaga atatcaatgc
    52621 aaatcacaag aggaaaacgt gcacgggctc aacgtgtggt catctatggt cctgaaggga
    52681 ttggtaagtc tagctttgct gctcaattcc cagagcctct gtttattgac actgagggat
    52741 caacagacaa tatggatgtg gcccgtatgg ataaaccaac aagctacact atgctaaaaa
    52801 atcaaattgc atggattaaa gcgaatccaa cttgttgcaa gacacttgtc cttgacacaa
    52861 ttgactgggc agaaagccta atcgttgatg atgtgtgtgc tcaacacggt aaaaagggaa
    52921 ttgaagattt tggctggggt aatggttata cctacaccaa agaagaggtt ggacgcttcc
    52981 taaacagtct gcaagaactg attgaatttg gtatcaatgt cgtccttact gcccatgccc
    53041 aaatgcgtaa atttgaacaa cctgacgaga tgggagctta tgaccgttgg gaattgaaac
    53101 ttggtaaaaa gacaagctca cagactgctc cgctcgtcaa ggaatgggct gatatggtac
    53161 tctttgctaa ttataaaact gttgttatga caactgatac taacaaaaaa aaggctactg
    53221 gcgggtctcg tgttttgtac acgcaacacc acccggcgtg ggatgctaag aaccgtcacg
    53281 ggttaccaga agaaatgcca ctcgattatg ctggtattgc tcatatcttt gctcagcaag
    53341 caccaagtca gccagtagca gaaacgccat caacacaaca accgacgcct actcaatccg
    53401 cacacgaacc cgcacataag caaccgacac aaccgccggt agcaaatgaa tcgcaggcac
    53461 cactgcccga cacattacca caagctatga aagatttgat ggtacctgat caagttacag
    53521 cggatgaact agtacaagtg gctaacatac gcggacattt cccgcttggg acgccgattg
    53581 aaaacttccc cccaaattat tgggatatga ttgttgcaaa ttggtcagca actcttgaag
    53641 ttatcaaaac acaagtaaga gtcgaaccag acttaccctt taccgtggaa gggacttaga
    53701 ttttgggaaa tagaaataat agcgaggtat aacaaaaatg gataaaacaa tcaaatttga
    53761 cttatctgcc attggtgatg gtggaaagtg gcgcttagat gctatcaata atattgctaa
    53821 ctacttaaaa gaagaacttg ctgatcaaac taacattaca attttagctt aatagaaaat
    53881 aggagaaata aaaatgactg aatacaataa caacttcgac catgaattgg gatgggatga
    53941 tgaaattaca caagacagtg taggttttgt ccaattagtg cctggtgatt atcaatttac
    54001 agtaactaat atggagcgtg gtcgtcacac acctgatccg cagaatccag gaaaattacc
    54061 agcgtgtaac aaggcggtcg ttacgattca aattgaaaca tcagaaggaa tcgcgcaatt
    54121 aactcataac ttattcctat atactacaac agaaggtatg ctatcagctt tctttggtgc
    54181 tattggtcaa aagaaacatg gggaaccact taaaatgaat tggaatactg tcgttggtgc
    54241 aaaaggtgtc tgtcgtgtaa ataagcgaaa aggaactggt aaatatgctg atcaagaata
    54301 cgataacatc aaatctatga tttatgcaga tgaagttgat tggtcgaaag ttttgaacgc
    54361 tagtcaacca acacaaaata cttatcaaca accgcaacaa ccaactcagc aacaacctca
    54421 accgcaacag ggaagtttct tccaaggtaa tacaacacaa ccacaaggtg gatggggagg
    54481 attctgatgc aattaaggcc gtatcaggaa gaggcacggt cagccgtaca aaaggaatgg
    54541 gaggaaggta gaaaacgaac tctactagtc ctccctaccg gttgcggtaa aactattgtc
    54601 ttttcgaaaa ttatcgaaga ccgtgtaagg ctcggagaga gagttttggt tctagctcat
    54661 aggtctgaac ttttagaaca ggctagtgat aaattgatga cagcaactgg attagggaca
    54721 gcattagaaa aagcagagaa tacttctttg gggtcatggt tcagggttgt agttggatca
    54781 gttcaaacga tgcagagaga gaagcgtttg agtaagtttc ctgcaaatca ttttgacaca
    54841 attattattg atgaggccca ccatgccatc tctgacggtt accaaagagt tttgcaacat
    54901 tttgatagta gcaatgtttt gggagttaca gcgacaccag atcgtggtga caaacaaaat
    54961 ctaggtaaat atttcgatag tttagcttat gaatattctt tggtagatgc catcaaatct
    55021 ggttatctat caaaaatcac tgctgttaca attccactat cactggactt aacgactgtt
    55081 agtcagcaag caggagactt taaagctagt gacatcggaa ctgccttaga tccatatctt
    55141 gagcagattg ccgatgaaat ggtgaagcaa tgtgcaaacc gtaaaacagt tgtatttctt
    55201 ccacttgtta aaacttccca aaaattccgt gatattctaa accaaaaagg attcaaagca
    55261 gcagaagtca atggagaatc caaagaccgt gctgaagtcc tagaagactt tgataatgat
    55321 aaatataatg tactatgcaa ttcaatgctt ttaacggagg gatgggattg tccaacagtg
    55381 gattgtgttg ttgtattgag accaactaaa gtacgagcac tgtatagcca aatggtaggg
    55441 cgtggcacac gtcttgcacc aggtaaggaa aatctattgc ttttagactt cctttggcac
    55501 acagaacgtc acgaactatg cagaccagcg catctgattg ctagtagccc agaagtggct
    55561 caaaagatgg tcgagaatat gactgaagaa accgaggtgg cttttgagct tatcgaggct
    55621 gaagaagtag ctgccaaaga tgtcgtagct gagcgcgagg aagcactggc caaacaattg
    55681 gcagaaatgc gcaagcgtaa acgtgctctt gttgatccgc tacaattcga aatgtctatc
    55741 caagctgaag atttagcaga ttatgtccca gcatttggtt gggagcaatc gccaccatct
    55801 gataaacagc tagaagcact tgaaaaattt ggcatttaca cagacgatat tggcaatgct
    55861 ggtaaagcta ctaaattatt agagcgattg gtcaagagga gagaaactgg cctaacaaca
    55921 cctaaacaaa tcagaacgct agaacgatac ggttttaaga atgtgggcat gtggaaattt
    55981 gaagtagcat caaacatgat taaccgcatt gctgctagcg gatggcgcct accgaaaggt
    56041 atcaatgctc gagaatacca acctgaatag gagacaaaat aaatggaaaa tatgaatgaa
    56101 caaattgaaa aatttattaa tgattttgta aatgaagcta tcgaaaaatc tgatacatac
    56161 gctgatgcca ttttgtatgt taataagatt gcatctctta cagagttagg ccaagtaatt
    56221 aaaaaagcta ttcaagacaa aatcggtgaa tgtgccttaa atagcaaaat taattaatat
    56281 cagacaaaga aaggataaca tggcagagaa tgattttaat ttgctaccgt tgctggatta
    56341 tatcaatcct gccacagtag actatcgaac ttgggtccaa gtaggaatgg ccttaaaaca
    56401 cgaaggatat actgctatgg attgggatgt ttggtcacaa tctgacagta gatataaaaa
    56461 aggcgaatgt ttcgctaaat gggatagctt tcaaggtaat gggtttggga ctgtcaccgg
    56521 tgcaactatc acacagttag caaaagaaaa cggttggaca tctgactacc gtacgagtga
    56581 tgaagcccac gagcttggtt gggatgacac aatcgatcgt gattataaaa ttgtggataa
    56641 gaactgggtt gaggctaagg aaattaaaga acctactaac tggtcaccag ttcacgattt
    56701 aattacttat atcgacactc tttttgaatc aactgacaaa gtgggctatg taacaaaaac
    56761 ctacccgatt gagacagatg aaggggtagt ctacaaacca acaaaagggg actacgcacg
    56821 tacagcaggc gaacttatcc aagagttgca aaaatacgga aacgatatcg gtgctgtgct
    56881 tggcgactac aaagaagaag ctggtgcatg gatacgattt aaccctttag atggaaatgg
    56941 tgtcaaaaac gacaatgtaa cagactttag atatgccttg gttgaatccg atagcatgga
    57001 acttgggaaa cagtatgctt tgtttaaaga actagaattg ccaatagcga cattggtcca
    57061 tagcggtaaa aaatcattac acgctattgt caaagtagat gctagagatt atcaggagta
    57121 ccgcaaacga gttgattaca tctatcaaat ctgcaagaaa aacggtcttg atattgacac
    57181 acagaatcgt aatccaagtc gtctatcacg aatgccgggt attatgcgaa atggtcacaa
    57241 gcaatttttg attgatacaa atattggtaa aaccaattat gaagaatggt atcaatggat
    57301 tgaagattta aatgatgatt taccagatcc agaaacgcta gctgatgaat gggataattt
    57361 gccagagctt gctcctgaat taattaaagg agtgttacgc cagggacata aaatgctaat
    57421 tgcaggacca tcgaaagctg gcaagtcatt tgctttgatt gagttatcga tagcacttgc
    57481 tgaaggcaaa gaatggttag gttgggactg tgaacaagga aaagttttat atgtcaattt
    57541 ggagctcgat agaccatcag ctttgcaccg ctttaaagat gtttatgatg caatgggatt
    57601 acctcctact aatgtggcaa atattgacat ttggaacctc cgtgggaaaa cagtaccaat
    57661 ggataagtta gcacctaaac ttatcagacg ctcacttaag aaaaattacc aagcggttat
    57721 tatcgaccct atttataaag ttctcactgg tgatgaaaac agtgctgatc agatggcaca
    57781 ctttaccaat caatttgaca aagtggccac tgaactagga tgctcagtta tctactgcca
    57841 ccaccattca aaaggcagtc aaggcggtaa aaaatctatg gaccgcgcca gtggttctgg
    57901 ggtattcgct cgcgaccccg atgctttgat tgatttagta gaacttgagc tgactgatga
    57961 gttaatcaag caacgtgttg aaaaggcatc tgctaaggtt tatcagaaag ctttgcagga
    58021 acagaacctt tcttactatc agcatgaagt gacattagat gatttggaaa gtcgctatca
    58081 aatgcaacaa cattttgaca aggcgatacc agacatcatg ctaaaacagc catatctgaa
    58141 cgaaatcaag agaattactc atgctattga aatcagtaca ggctggcgag ttgaagggac
    58201 tttacgtgag tttgctaaat ttcagccggt caacatgtgg tttagctatc cagtgcacga
    58261 agttgataca actggtgtgt tggcagatat acaactggag gaaagtaagc cgaactggca
    58321 gaaagctaag gaaggtagaa agtcaaaaaa agaaaaggca gaggaacgta agcaaaaaat
    58381 agatacagcc tacagtgctc tatttgatgg cttcacaccc gtttcattat cagagttgca
    58441 agagtatctg gattctaccg aaaaaacagt tcgaaatcat ctcaaagagc atggtggttt
    58501 tgatattaaa aacggaattg tctatcagaa aaaaacagaa tagggaaaga aaataacaga
    58561 aaattttccc tgtaaaaaac agagaatttt atgttatttt cccttcctaa atcctgtaaa
    58621 aaacataaat tttccctttt tctaaatcct gtaaaaaaca gagaatttta tgttattttc
    58681 cggaaaaaga cacgctataa ctcttatcag agttattaaa agtgtttttc cttcgtcaaa
    58741 agtcaaagaa aaaggaaggt ttggcgttag acttcgccaa aaccattcct ttctcatctt
    58801 tgacaaagcg cgtagattaa gaaaggaata aaaaatgcaa attgaatttt ttaaaaatgg
    58861 taaaagtgat attgagttgg aatatttaaa aaatgaagtt aacggtttta taaacgacct
    58921 gcgagaagat ggtattaaat gtttagatat ccaattcatt tttgatgaag atgagatcat
    58981 cgtatttgtt aaatatgagg gcttaatcga tgattgattt ctttatcccg atgaagaaaa
    59041 tcccaactac tacacaccaa caaaagaagg tccgtgtggt aaacggtaaa ccacaattct
    59101 acgaaccgga ttcgctgaag gaagctcgtg ccatgtttat gagtaaattt gcaccccatg
    59161 ctcctgagaa aaaaatgacc ggtccattac gactcactgt caaatggtta ttcccgaaaa
    59221 tcaaaaaatc agtagacgga caatataaag atactaaacc cgacacagat aatttgaaca
    59281 agttacctaa agattgtatg actgcattgg gattttggaa tgatgatgcg caggttgctt
    59341 cggaaattat tgagaagttc tgggctgata cagtaggaat atacgtaaag gtggaagagt
    59401 tatgaaaatt gattatatta atttttttga acgagtggtt cctgactgga tgagagagag
    59461 taatgtcaaa atgcaagaag ttggatttaa tactgaagct tattggatat gggccaacca
    59521 gtcaattgtt acgatttgcg aaacttatgg taacgactca ttaattaatg gtcaatttca
    59581 tcttatttgg gagtggcttg aaagtaaaac aaaagaaagt agttaaaaat ggtaaaacac
    59641 gttgttagag tttacaacaa aggtattaca gcgacttacg cgatctatga caaaaacctg
    59701 tttcaggagc atgagtttgt aacaaaaata gaagcaatgc agtttattag acggctagag
    59761 ttagctaatg ataagcgagc gacagaatat tttatgagag aggtagacga atgatactaa
    59821 caaaagattt agcgaaaaaa cacttaggat gctatgttga cagatataga cgtaatgacg
    59881 ttatcaacgg catgtttccg aagagaatag tagagttatc tgacgggcga ttatacctca
    59941 ggaatccgat tggtgtatat acaaaaatag aagaagattt agatattgac tatattttta
    60001 aaccaataaa agttttagag tttttgtcag aaactgaggt taacgatgat accaaaattt
    60061 agggcattta ataaaaagac caaaaaaatg tatagcattg atggctttaa atcaagtgaa
    60121 cgcaaaatat acagatgcag cttagctgat gatgagtttc gctctggtcg cttagagacg
    60181 tttcattttg tcgaggataa ccttgacgat tatattctca tgcaatcaac aggtctaaaa
    60241 gataaaaacg gcattgaggt gtttgatggg gatataatgt tttacgaaca ggattgctat
    60301 caatacacat tagtcaaata tgataaagat aagcttgctt ttgtgttata cgatggttgc
    60361 gaaagacttt atcacgaatt gtgggaacca ggagaagtca ttggcaacat ctacgaaaat
    60421 ccaaaattac tagaaagtgt ggaagaatga caacagatga attattgcaa aatttacgtg
    60481 atgacttgaa taaaataatg aatgtcctaa aaaacgattg gaaagcacta ttgtttcttg
    60541 caatcgcaat atttgggatg atggtaaccg tgtcgtattt tagctatcgc gacgcacgac
    60601 aatattacga gtcgcaaatc acaggactac gtacacagct aagcaggaca caaaagcagc
    60661 ttaaacgtgc tagcgaagat agagctagac agacaaagcg aattgcggaa
//
LOCUS       DATXQK010000008        53523 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.008, whole genome shotgun sequence.
ACCESSION   DATXQK010000008 DATXQK010000000
VERSION     DATXQK010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 53523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53523
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.008"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(448..1854)
                     /gene="lacG"
                     /locus_tag="VC394_000499"
     CDS             complement(448..1854)
                     /gene="lacG"
                     /locus_tag="VC394_000499"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEP5993993.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLSEEFGVNGIRISIAWSRIFPTGKGEVNPKGVEYYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSDGDFLNRENIEHFVNYAEFCFKEFSEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLFKDSGYSGEIGVVHAL
                     PTKYPFDANNPDDVRAAELEDIIHNKFILDATYLGKYSDKTMEGVNHILEVNGGELDL
                     REEDFAALDAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     KAPVDVPKTDWDWIIFPQGLYDQIMRVKADYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVISDAISDGANVKGYFMWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAYWYKKVAETQVIE"
     gene            complement(1927..3624)
                     /locus_tag="VC394_000500"
     CDS             complement(1927..3624)
                     /locus_tag="VC394_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEP5993994.1"
                     /translation="MNGLIAQIEKGKPFFEKISRNIYLRAIRDGFIAGMQVILFSSIF
                     ILIAYVPNAWGFHWSKDIENLLMTPYNYSMGILGLFVAGTTAKALTDSMNRSLPSTNQ
                     INFISTMLAAIVGFLLMAANPAKDGGFLTGFMGTKGLLTAFIAAFITVNVYKTCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTLSVVLLYVIELIVHTTLGVNVAESIGKLLAPLFQ
                     AADGYVGITIIFGAFAFFWFVGIHGPSIVEPAIAAITYANAETNLQLLQAGEHADKIL
                     TSGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNKAIGRASVVPTFFGVNEPILFGAPL
                     VLNPIFFIPFILAPIVNVWIFKFFIDTLGMNSFTANLPWTTPAPLGLILGTNFQVLAF
                     ILAALLIVVDVIIYYPFLKVYDEQILAEEAAGTNSSDALKEKVAANFDTKKADAILEK
                     SAAKETKEITDQTNVLVLCAGGGTSGLLANALNKAAKEYGAPVTAAAGSYGAHREILP
                     QYQLVILAPQVASNYEDMKVETDKLDIKLAKTEGAQYIKLTRDGQGALDFVKEQMEK"
     gene            complement(3624..3941)
                     /locus_tag="VC394_000501"
     CDS             complement(3624..3941)
                     /locus_tag="VC394_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEP5993995.1"
                     /translation="MNREETTLLGFEIVAYAGDARSKFLEALKAAQEGDYAKAEELIA
                     AGSDCLNDAHNAQTSLLQKEAAGDDLAYSVTLMHGQDHLMTTILLQDLMKHMIELYKR
                     GAK"
     gene            complement(3965..4948)
                     /gene="lacD"
                     /locus_tag="VC394_000502"
     CDS             complement(3965..4948)
                     /gene="lacD"
                     /locus_tag="VC394_000502"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEP5993996.1"
                     /translation="MTITLTENKRKSMEKLSIDGVISALAFDQRGALKRMMAQHQTKE
                     PTVEQIEELKSLVSEELTPFASSILLDPEYGLPASRVRSEEAGLLLAYEKTGYDATTT
                     SRLPDCLDVWSAKRIKEAGAEAVKFLLYYDIDGDQDVNEQKKAYIERIGSECRAEDIP
                     FYLEILTYDEKIADNASPEFAKVKAHKVNEAMKVFSEERFGVDVLKVEVPVNMKFVES
                     FADGEVLFTKEEAAQAFRDQEASTDLPYIYLSAGVSAKLFQDTLVFAAESGAKFNGVL
                     CGRATWAGSVKVYIEEGPQAAREWLRTEGFKNIDELNKVLDKTASPWTEKM"
     gene            complement(4951..5880)
                     /locus_tag="VC394_000503"
     CDS             complement(4951..5880)
                     /locus_tag="VC394_000503"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEP5993997.1"
                     /translation="MILTVTLNPAIDVSYPLNELKCDTVNRVVDVTKTPGGKGLNVSR
                     VLNDFGETVKATGCIGGESGDFIINHLPDSILSRFYKISGDTRTCIAILHEGNQTEIL
                     EKGPLLSVDEIDGFTHHFKYLLNDVDVVTLSGSLPAGMPDDYYQKLIKIANLNGKKTV
                     LDCSGNALEAVLKGDSKPTVIKPNLEELSQLLGKEMTKDFEALKEVLQDELFEGIEWI
                     IVSLGADGVFAKHKDTFYNVDIPKIKIVSAVGSGDSTVAGIASGLANDEDDRALLTKA
                     NVLGMLNAQEKTTGHVNMANYDKLYQSIKVKEV"
     gene            complement(5926..6441)
                     /gene="lacB"
                     /locus_tag="VC394_000504"
     CDS             complement(5926..6441)
                     /gene="lacB"
                     /locus_tag="VC394_000504"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEP5993998.1"
                     /translation="MKIAVGCDHIVTYEKIAVVDYLKSQGHEIIDCGTYDNVRTHYPI
                     FGKKVGEAVASGEAELGVVICGTGVGITNAVNKVPGIRAALVRDMTSAIYSKEELNAN
                     VIGFGGKIIGGLLMNDIIDAFLAAEYKPTEENKKWIEKMDSLQHASQDQNNPHFFDEF
                     LEKWDRGEYHD"
     gene            complement(6476..6904)
                     /gene="lacA"
                     /locus_tag="VC394_000505"
     CDS             complement(6476..6904)
                     /gene="lacA"
                     /locus_tag="VC394_000505"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEP5993999.1"
                     /translation="MAIIIGADKAGQELKEVIKDYLKEGKYEVVDVSENEVRDFVDTT
                     LAVAKEVNASEDNLGIVIDAYGVGSFMVATKIKGMVAAEVSDERSAYMTRGHNNARII
                     TLGSEISAPGIAKNIIKGFVEGKYDGGRHQVRVDMLNKMC"
     gene            7350..8123
                     /locus_tag="VC394_000506"
     CDS             7350..8123
                     /locus_tag="VC394_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004270277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEP5994000.1"
                     /translation="MKKKERHEKILDILKVDGFIKVKDIIDEMNISDMTARRDLDTLA
                     DKGLLIRTHGGAQYLDYSSAKDEGHEKTHTEKKVLQTTEKKQIAQRAKNIIKDGETVF
                     IGPGTTLEHLAVELKNRNIRVITNSLPVFLILHPSTTIDLILIGGECREITGAFVGAI
                     AINNLESLTFSKAFVSANAVYNNSIATYSDLEGEVQRVALNNAVEKILLVDSTKFESY
                     DFYNFYQLDQVDLIVTDKNVTTDIIEKYKKFSKIIIADK"
     gene            8823..9059
                     /locus_tag="VC394_000507"
     CDS             8823..9059
                     /locus_tag="VC394_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994001.1"
                     /translation="MVSEAMTVLNKKNLTLSSALRLFLQNVVVTNEVDLLTEEELEKE
                     KLFKQFQAEINKNIEDVRQGKFYTSEEVRAELGL"
     gene            9049..9384
                     /locus_tag="VC394_000508"
     CDS             9049..9384
                     /locus_tag="VC394_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEP5994002.1"
                     /translation="MDYKKYQIIYAPDVLEKLKEIRDYISQNYSSTSGQRKMEQIISD
                     IEKLEVFREVGFDADEKYGSKIIHYHSTKGYTLSKDYIVLYHIEGEENRVVIDYLLPT
                     QSDYIKLFK"
     gene            9512..10006
                     /locus_tag="VC394_000509"
     CDS             9512..10006
                     /locus_tag="VC394_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase family
                     protein"
                     /protein_id="HEP5994003.1"
                     /translation="MYLKASGNLEEFTKPTHKTYSEIFEEWYQAYQDTVEPTTASRTL
                     DLFQLHILPVMGELPINKTSPLDCQNFITDKAKTFKNIKQIKSYTEKIFDFAIKMKLL
                     KHNPMAEIIMPKRKKTRIENYWTVQELQEFLAIVLQKEPYKHYALFRLLAYSGLKERR
                     AMRS"
     gene            9969..10331
                     /locus_tag="VC394_000510"
     CDS             9969..10331
                     /locus_tag="VC394_000510"
                     /inference="COORDINATES: protein motif:HMM:NF012798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEP5994004.1"
                     /translation="MAALRKGELCALKWANIDFQSETLSVNKSLGRLDGQAIEKSTKN
                     DFSVRKIKLDSETISILQEWKAISQKEKAQLAVAPLSIESDFIFTYCTREGSIEPLHT
                     DYINNILSKVIKQHSLKK"
     gene            10367..10516
                     /locus_tag="VC394_000511"
     CDS             10367..10516
                     /locus_tag="VC394_000511"
                     /inference="COORDINATES: protein motif:HMM:NF012798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994005.1"
                     /translation="MIEIGVDPVNTAKRLGHASSQITLDTYSHATAVGEDKSIKQFAN
                     YLKNN"
     gene            complement(10649..11041)
                     /gene="rpsI"
                     /locus_tag="VC394_000512"
     CDS             complement(10649..11041)
                     /gene="rpsI"
                     /locus_tag="VC394_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEP5994006.1"
                     /translation="MAQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLIINQPFAVTSTEGSYDVFVNVVGGGYGGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(11062..11508)
                     /gene="rplM"
                     /locus_tag="VC394_000513"
     CDS             complement(11062..11508)
                     /gene="rplM"
                     /locus_tag="VC394_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEP5994007.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKVYYTHSMYPGGLKSITAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGGEHTHAAQQPEVLDISGLI"
     gene            complement(11726..11932)
                     /locus_tag="VC394_000514"
     CDS             complement(11726..11932)
                     /locus_tag="VC394_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP5994008.1"
                     /translation="MKNLKLKAARAGKDLSQQALADLVGVSRQTIAAVEKGDYNPTIN
                     LCIAICRVLDKTLDDLFWEVDDSH"
     gene            complement(11929..12627)
                     /locus_tag="VC394_000515"
     CDS             complement(11929..12627)
                     /locus_tag="VC394_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994009.1"
                     /translation="MFDSKQNLAKSQWGFIVITAVMDLVVIVATLLAKTSFQRYLVGG
                     VAVILTSFLILLVWGLKTAKKLQTRLDSKEVLDSKVRDDQKLPRYDERQKQILLKGYT
                     IGFWFMIVAVWLSIFLHRFSERLVSASFLFTLALWGGLAVQTTYCNLNGVSPFVDRRF
                     GKQGILVGMIATGMSLITIGLAVREMMAGQLKMGSFFKAGGSGSLMVLGITLLSMGAS
                     IFYRHYLDAKEADE"
     gene            complement(12763..13623)
                     /locus_tag="VC394_000516"
     CDS             complement(12763..13623)
                     /locus_tag="VC394_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEP5994010.1"
                     /translation="MTFTIMTDSTADLNQTWAEDHDIVLIGLTILCDGEVYETVGPNR
                     ISSDYLLKKMKAGSHPQTSQINVGEFEKVFREHARNNKALLYLAFSSVLSGTYQSALM
                     ARDLVREDYPDAVIEIVDTLAAAGGEGYLTILAAEARDSGKNLLETKDIVEAVIPRLR
                     TYFLVDDLFHLMRGGRLSKGSAFLGSLASIKPLLWIDEEGKLVPIAKIRGRQKAIKEM
                     VAQVEKDIADSTVIVSYTSDQGSAEKLREELLAHENISDVLMMPLGPVISAHVGPNTL
                     AVFVIGQNSR"
     gene            complement(13720..14238)
                     /locus_tag="VC394_000517"
     CDS             complement(13720..14238)
                     /locus_tag="VC394_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEP5994011.1"
                     /translation="MKKRILLVDGYNMIAFWQSTRQLFKINQLDQARNTLLTKLNHYA
                     HFENINIICVFDAQYVPGLRQRYDQYYISVVFTEEDETADSYIERMAAELNTAIHMVE
                     VATSDLNEQWTIFSQGALRVTARELEQRVHTVKADLDKMSRDIDLKTPKLRPFDQGQL
                     IQLKDFMSQLDR"
     gene            complement(14242..14988)
                     /gene="rlmB"
                     /locus_tag="VC394_000518"
     CDS             complement(14242..14988)
                     /gene="rlmB"
                     /locus_tag="VC394_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEP5994012.1"
                     /translation="MEDKDTIETNDIVYGVHAVTESLQANTGNKLYIQEDLRGKKVDN
                     IKSLATQKKVAISWTPKKTLSQMTDGAVHQGFVLRVSAFAYTDVDEILEIAEQEANPL
                     ILILDGLTDPHNLGSILRTADATNVCGVIIPKHRSVGVTPVVSKTSTGAVEHIPIARV
                     TNLSQTLDKLKARGFWIFGTDMNGTPSDCWNTNGKLALVIGNEGKGISTNIKKQVDEM
                     ITIPMNGHVQSLNASVAAAILMYEVFRNRR"
     gene            complement(15035..15832)
                     /locus_tag="VC394_000519"
     CDS             complement(15035..15832)
                     /locus_tag="VC394_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994013.1"
                     /translation="MTTMQKTVSLLSLALLIGLLGTSGKAISVYAQDQHTDNVIAEST
                     ISQVSVEASIRGTEPYIDATVTTDQPVRQPTQATITLKDASDNTINSWVYTMAAQQRR
                     FTAWFDLTGQKSGDYHVTVTVHTQEKAVTGQSGTVHFDQNKARKTPTNMQQKDTSKAT
                     TNSVDVDTKAQTNQSANQEIDSTSNPFKSATNHRSTSLKRSTKNEKLTPTASNSQKNG
                     SNKTKMLVDKEEVKPTSKRGFPWVLSGLVVSLAAGLFIAIQKVSRRK"
     gene            complement(16028..16441)
                     /locus_tag="VC394_000520"
     CDS             complement(16028..16441)
                     /locus_tag="VC394_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEP5994014.1"
                     /translation="MINPVDVNLINGIALAFEGDAVYSYYVRRHLIFQGKTKPSQLHR
                     LATRYVSAKAQANLIQAMLEAQLLTEKEEDIYKRGRNTNSHTKAKNADIITYRMSTGF
                     EAIMGYLDMMGQKERLEELIRWCIEYVEKQQLISS"
     gene            complement(16434..17777)
                     /locus_tag="VC394_000521"
     CDS             complement(16434..17777)
                     /locus_tag="VC394_000521"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000591125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEP5994015.1"
                     /translation="MIKIYDTMTRSLRKFVPLTENTVNMYVCGPTVYNYIHIGNARSA
                     VAFDTIRRYFEYTGYQVNYISNFTDVDDKIIKAATQAGVSPKELSDRFIAAFIEDTKA
                     LGVKPATQNPRVMDYIAEIISFVESLIEKDFAYEADGDVYFRVEKSEHYAKLANKTLS
                     ELEVGASGRTDAETALKENPLDFALWKSAKAGEVSWDSPWGFGRPGWHIECSVMATEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKTFANYWMHNGFVTVDNEKMSKSLGNF
                     VTVHDMLQTVDGQVLRFFLATQQYRKPINFTEKAIHDAEINLKYLKNTLQQPLTETAD
                     EQELKQFVIAFQDAMDDDFNTANGITVVFDMAKWINSGSYTEPVKSAFEKMLAVFGII
                     FEEEVLEVDIEALIAKRQEARANRDFAIADAIRDQLAAQGIKLLDTKDGVRWLRD"
     gene            complement(17805..18035)
                     /locus_tag="VC394_000522"
     CDS             complement(17805..18035)
                     /locus_tag="VC394_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994016.1"
                     /translation="MNKIRVGLYVIMSLSILVSSITLVASLIFLLRQDMVKAIIYFIL
                     TLFWLIGLVINIKLLKRWNSISEKDLGYQDDN"
     gene            complement(18514..19095)
                     /gene="cysE"
                     /locus_tag="VC394_000523"
     CDS             complement(18514..19095)
                     /gene="cysE"
                     /locus_tag="VC394_000523"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEP5994017.1"
                     /translation="MGWWKESIAIVKALDPAARNSLEVILTYPGIKALAAHRLSHFLW
                     RHHFKLLARMHSQFWRFWTQIEIHPGAQIAPGVFIDHGAGLVIGETAIVEKGVMLYHG
                     VTLGGTGKDCGKRHPTVRQGALISAHAQVIGPIDIGANAKVGAAAVVLSDVPEDVTVV
                     GVPAKIVRVHGQKDNRQIQSLQKQREVSYQLSK"
     gene            complement(19104..19856)
                     /locus_tag="VC394_000524"
     CDS             complement(19104..19856)
                     /locus_tag="VC394_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HEP5994018.1"
                     /translation="MTKSNELDIRLRAFINAPDNFLDSVALVNAFHNFPVWAAKEPYV
                     IEVEGVKVTPVFTDKEDMARFKEEQESAQSQYWLERSALAVLEEVITSGAAGLVFNLK
                     KKGDFGNSTIFKSSDMIQFMNHYTTVLNTLMSDDNVAADTMEKVYLVPAFVYPKDNNH
                     YDRLFPTMSTPEGKSYVPAFSNLQSFAKWYNQDDFGGLFRKAEGVILTWTIDDIYQPR
                     NGENELDETFGVAINPFDDQQILVDWSELDKS"
     gene            complement(19849..21981)
                     /gene="pnp"
                     /locus_tag="VC394_000525"
     CDS             complement(19849..21981)
                     /gene="pnp"
                     /locus_tag="VC394_000525"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEP5994019.1"
                     /translation="MSKQTFTTTFAGKPLVVEVGQVAKQANGATVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFNGPIAGVQVGYIDGEFI
                     INPDKEQMEASLLELTVAGSKEAINMVESGAKELSEDIMLEALLKGHQAIQELIAFQE
                     QIVAVVGKEKAEVELLQVDADLQADIVAKYNAQLQKAVQVEEKKAREAATEAVKEMVK
                     AEYEERYAEDENLATIMRDVAEILEQMEHAEVRRLITEDKIRPDGRKIDEIRPLDAVV
                     DFLPKVHGSGLFTRGQTQALSILTLAPMGETQIIDGLAPEYKKRFLHHYNFPQYSVGE
                     TGRYGAAGRREIGHGALGERALEQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGTNYTVLTDIQGLEDHFGDMDFKVAGTREGITAL
                     QMDIKIAGITPQILEEALAQAKKARFEILDVIEATIAEPRPELAPTAPKIDTIKIDVD
                     KIKVVIGKGGETIDKIIAETGVKIDIDDEGNVSIYSSDQAAINRTKEIIAGLVREAKV
                     GEVYHAKVVRIEKFGAFVNLFDKTDALVHISEIAWTRTTNVSDVLEVGEDVDVKVIKI
                     DEKGRVDASMKALIPRPPKPEKKEEKHD"
     gene            complement(22261..22992)
                     /locus_tag="VC394_000526"
     CDS             complement(22261..22992)
                     /locus_tag="VC394_000526"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEP5994020.1"
                     /translation="MMTKKLNVKVFSDGAVLETMLKDLQTGLVTGFTTNPSLMKKAGI
                     SSYIGFAKEVLAKITDYPVSFEVFADDLASMEKEAEKIASLGDNVYVKIPVTTSTGES
                     TCPVIQKLSAKGIKLNVTAIFTIEQTQAVVDHLTAGVPAIVSVFAGRIADTGVDPMPI
                     MEEALRICRQKEEVELLWASPRETYNIYQADQLGVDIITCTTDLIAKLPLQGKDLEDY
                     SLETVQMFLKDSTSLGFKILEDDNH"
     gene            complement(23002..24381)
                     /locus_tag="VC394_000527"
     CDS             complement(23002..24381)
                     /locus_tag="VC394_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEP5994021.1"
                     /translation="MEALLSFIRDILKEPAFLMGLIAFAGLVALKTPAHKVLTGTLGP
                     ILGYLMLAAGAGVIVTNLDPLAKLIEHGFSITGVVPNNEAVTSVAQKILGVETMSILV
                     VGLLLNLAFARFTRFKYIFLTGHHSFFMACLLSAVLGAVDFKGSLLIILGGFLLGAWS
                     AISPAIGQQYTLKVTDGDEIAMGHFGSLGYYLSAWVGSKVGKDSKDTEDLQISEKWSF
                     LRNTTISTGLIMVLFYLVATVASVLRNTSVTEELAAGQNPFIFAIKSGLTFAVGVAIV
                     YAGVRMILADLIPAFQGIANKLIPNAIPAVDCAVFFPYAPTAVIIGFASSFVGGLLGM
                     LILGVAGGVLIIPGMVPHFFCGATAGIFGNSTGGRRGAMIGAFANGLLLAFLPAMLLP
                     VLGKLGFSNTTFGDVDFGVLGILLGRLGTSIGQIGIYLVVAVLAIVLVLPNFLSKSQE
                     AINNVSEEI"
     gene            complement(24408..24692)
                     /locus_tag="VC394_000528"
     CDS             complement(24408..24692)
                     /locus_tag="VC394_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEP5994022.1"
                     /translation="MIKIVTVCGNGIGSSLLLRMKVEAIASSLGIDVDAESCDSNAAV
                     GKGADLFVTVKEFKDIFPEDAKVCIVKSYTNRKKIEEDLVPVLKEMSGKE"
     gene            complement(24685..26745)
                     /locus_tag="VC394_000529"
     CDS             complement(24685..26745)
                     /locus_tag="VC394_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEP5994023.1"
                     /translation="MLSHELIRNYQLFSKYKGHSLEAFESILKTSKRHILADIAKIND
                     TLSLYQLPLIALDRQLVYPPDLTEKDLLNRMLPTLDDYLFQDERLDMIIIYIMMAKEF
                     ISINHLESLLRLSRNSVIADLNLVRDRVQAFQVTLAYNRQDGYFFEGEPLALRRLLES
                     AVSSLLQVTSGPWVFSYLLHELGLPDQTKVMAATLEELSRENHLTFISEKLRDLIYFF
                     CLLAHRPFSRNVRAEVVDTFPLASPAVETMVDQLLVNFPSLTEEKYLVQSRLLGCIQG
                     DLELVFQQPIYDIMEEIINSVAVNTGLSITDTPELRQNLYSHLLPAYYRLYYDINLTN
                     PLKEQIKQDYESLFYLVKRSLSPLEKQLGKPVNEDEIAYFTIHFGRWLQAPKKRPSNQ
                     LVALSVCPNGISSSLMLEATLKELFPQLQFIRIHQLDKIKLLDPASFDLIFSTVAFDC
                     VKPVYVTQALMGPVEKMMLKKMVCDDFHLPLSEQFALDDLLSIIHKHTTITNKEGLVS
                     DLSRYLFGNHLTIEKGGLGLLDLLTADFIRQADAVSDWQEAIRLAAQPLLEHQMIETS
                     YIDGMIDSVNELGAYIVLAPKVAVPHAAPEKGTRQLGMSLLQLKEPVSFDLKQEGDPD
                     KQVQLIFVLSAVDSSSHLKALQELSLILDDDEHIEQLIEAKNTEEIMSLISHMIEKGD
                     ESHD"
     gene            complement(27098..27367)
                     /gene="rpsO"
                     /locus_tag="VC394_000530"
     CDS             complement(27098..27367)
                     /gene="rpsO"
                     /locus_tag="VC394_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEP5994024.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNSHIK
                     EHKKDHATYRGLMKKIGHRRNLLAYLRRTDVNRYRELIQSLGLRR"
     gene            complement(27525..27680)
                     /locus_tag="VC394_000531"
     CDS             complement(27525..27680)
                     /locus_tag="VC394_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994025.1"
                     /translation="MTMLGYRLSAEVRGSAILPLLAGYLIRQAIAQVYSDLKTASWLF
                     GLASSVA"
     gene            27806..28063
                     /locus_tag="VC394_000532"
     CDS             27806..28063
                     /locus_tag="VC394_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEP5994026.1"
                     /translation="MTQNVCQQNITYTVKERFASYTLEAQANQEVHLNLTLLANRFGT
                     SDRHLKHVLKQPIFQRIIERICFKTCRIADLDALTQLSVNN"
     gene            28189..28803
                     /locus_tag="VC394_000533"
     CDS             28189..28803
                     /locus_tag="VC394_000533"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEP5994027.1"
                     /translation="MSAQDKLIKPSHLITMDDIIREGNPTLRAVAKEVSLPLCDEDIL
                     LGEKMMQFLKHSQDPVMAEKLGLRAGVGLAAPQIDVSKRIIAVLVPNLPDKEGNPPKE
                     AYSWQEVLYNPKIVSHSVQDAALSDGEGCLSVDRVVEGYVVRHARVTVDYYDKEGQQH
                     RIKLKGYNAIVVQHEIDHINGVLFYDRINAKNPFETKEELLILD"
     gene            complement(28836..29378)
                     /locus_tag="VC394_000534"
     CDS             complement(28836..29378)
                     /locus_tag="VC394_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEP5994028.1"
                     /translation="MKHILFIVGSLREGSFNHQLAAQAQKALEHQAVVSYLNWKDVPV
                     LNQDIEANVPLPVVDARQAVQSADAIWIFTPVYNFSIPGSVKNLLDWLSRALDLSDPT
                     GPSAIGGKVVTVSSVANGGHDQVFDQFKALLPFIRTSVAGEFTKATVNPDAWGTGRLE
                     ISKETKANLLSQAEALLAAI"
     gene            complement(29509..29937)
                     /locus_tag="VC394_000535"
     CDS             complement(29509..29937)
                     /locus_tag="VC394_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEP5994029.1"
                     /translation="MSHLDKNTALKAMVVFRKAQRTLDAFGADIFKKADLTATQFSVL
                     EVLYTKGCMRINHLIDSLLATSGNMTVVLNNMERNGWISKCKDKTDKRAYVVTLTDKG
                     TRLIEAVLPKHVARVEEAFAVLTEKEQLCLIELLKKFKQL"
     gene            complement(30047..34444)
                     /locus_tag="VC394_000536"
     CDS             complement(30047..34444)
                     /locus_tag="VC394_000536"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEP5994030.1"
                     /translation="MSDLFAKLMDQIEMPLDMRRSSAFSSADIIEVKVHSVSRLWEFH
                     FAFAAVLPIATYRELHDRLIRTFEAADIKVTFDIQAAQVDYSDDLLQAYYQEAFEHAP
                     CNSASFKSSFSKLKVTYEDDKLIIAAPGFVNNDHFRNSHLPNLVKQFEAFGFGTLTID
                     MVSDQEMTEHLTKDFVSSRQALVKKAVQDNLEAQKSLEAMMPPVEEATPAPKFDYKER
                     AAKRQAGFEKATITPMIEIETEENRIVFEGMVFDVERKTTRTGRHIINFKMTDYTSSF
                     ALQKWAKDDEELRKFDMIAKGAWLRVQGNIETNPFTKSLTMNVQQVKEIVHHDRKDLM
                     PEGQKRVELHAHTNMSTMDALPTVESLIDTAAKWGHKAVAITDHANVQSFPHGYHRAR
                     KAGIKAIFGLEANIVEDKVPISYDPVDMDLHEATYVVFDVETTGLSAMNNDLIQIAAS
                     KMFKGNIVEQFDEFIDPGHPLSAFTTELTGITDKHLQDAKPLVTVLKAFQDFCKDSIL
                     VAHNASFDVGFMNANYERHDLPKITQPVIDTLEFARNLYPEYKRHGLGPLTKRFQVSL
                     DHHHMANYDAEATGRLLFIFLRDAREKHGIKNLLQLNTDLVAEDSYKKARIKHATIYV
                     QNQVGLKNMFKLVSLSNIKYFEGVPRIPRTVLDAHREGLLLGTACSDGEVFDAVLTKG
                     IDAAVDLAKYYDFIEIMPPAIYQPLVVRELIKDQAGIEQVIRDLIEVGKRANKPVLAT
                     GNVHYLEPEEEIYREIIVRSLGQGAMINRTIGRGEGAQPAPLPKAHFRTTNEMLDEFA
                     FLGKDLAYQVVVENTQDFADRIEEVEVVKGDLYTPYIDKAEETVAELTYQKAFEIYGN
                     PLPDIIDLRIEKELTSILGNGFAVIYLASQMLVNRSNERGYLVGSRGSVGSSFVATMI
                     GITEVNPMPPHYVCPSCQHSEFITDGSVGSGYDLPNKPCPKCGTPYQKDGQDIPFETF
                     LGFDGDKVPDIDLNFSGDDQPSAHLDVRDIFGDEYAFRAGTVGTVAEKTAYGFVKGYE
                     RDYGKFYRDAEVDRLAAGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAS
                     WQTTHFNFHDIDENVLKLDILGHDDPTMIRKLQDLSGIDPITIPADDPGVMALFSGTE
                     ILGVTPEQIGTPTGMLGIPEFGTNFVRGMVNETHPTTFAELLQLSGLSHGTDVWLGNA
                     QDLIKEGIATLKTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGY
                     IDAMRENNVPDWYIESCGKIKYMFPKAHAAAYVLMALRVAYFKVHHPIMYYCAYFSIR
                     AKAFELKTMSGGLDAVKARMEDITIKRKNNEATNVENDLFTTLEIVNEMLERGFKFGK
                     LDLYKSDAIEFQIKGDTLIPPFIALEGLGENVAKQIVKARQEGEFLSKMELRKRGGAS
                     STLVEKMDEMGILGNMPEDNQLSLFDDFF"
     gene            complement(34698..36554)
                     /locus_tag="VC394_000537"
     CDS             complement(34698..36554)
                     /locus_tag="VC394_000537"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEP5994031.1"
                     /translation="MKQSKLLIPTLREMPSDAQVISHALMVRAGYVRQVSAGIYAYLP
                     LANRTIEKFKTIMREEFEKIGAVEMLAPALLTADLWRESGRYETYGEDLYKLKNRDNS
                     DFILGPTHEETFTTLVRDAVKSYKQLPLNLYQIQSKYRDEKRPRNGLLRTREFIMKDG
                     YSFHHNYEDLDVTYEDYRQAYEAIFTRAGLDFKGIIGDGGAMGGKDSQEFMAVTPART
                     DLDRWVVLDKSIASMDDIPKEVLEDIKAELAAWMISGEDTIAYSTESSYAANLEMATN
                     EYKPSSKVAAEDALAEVETPHCKTIDEVAAFLSVDETQTIKTLLFVADNEPVVALLVG
                     NDHINTVKLKNYLAADFLEPASEEEARAFFGAGFGSLGPVNLAQGSRIVADRKVQNLT
                     NAVAGANKDGFHVTGVNPGRDFQAEYVDIREVKEGEMSPDGHGVLQFARGIEVGHIFK
                     LGTRYSDSMGATILDENGRTVPIVMGCYGIGVSRILSAVIEQHARLFVNKTPKGDYRY
                     AWGINFPKELAPFDVHLITVNVKDQVAQDLTAKLEADLMAKGYDVLTDDRNERVGSKF
                     SDSDLIGLPIRVTVGKKAAEGIVEIKIKATGDSIEVNAENLIETLEILTKEH"
     gene            complement(36752..38011)
                     /gene="rseP"
                     /locus_tag="VC394_000538"
     CDS             complement(36752..38011)
                     /gene="rseP"
                     /locus_tag="VC394_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEP5994032.1"
                     /translation="MLGIITFIIIFGILVIVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FSHVDQGGTLYTLRMLPLGGYVRMAGWGDDKTEIKTGTPASLTLNEQGFVKRINLSQS
                     KLDPTSLPMHVTGYDLEDQLSITGLVLDETKTYKVAHDATIVEEDGTEIRIAPLDVQY
                     QNASIGGRLITNFAGPMNNFILGIVVFILLVFLQGGMSDFSSNHVRVQENGAAAKAGL
                     RDNDQIVAINGYKVTSWNDLTEAVDLATRDLGPSQTIKVTYKSHQRLKTVAVKPQKHA
                     KTYTIGVKASLKTGFKDKLLGGLELAWSGAFTILNALKGLITGFSLNKLGGPVAMYDM
                     SNQAAQNGLESVLSLMAMLSINLGIFNLIPIPALDGGKILMNIIEAIRRKPIKQETEA
                     YITLAGVAIMVVLMIAVTWNDIMRVFF"
     gene            complement(38084..38878)
                     /locus_tag="VC394_000539"
     CDS             complement(38084..38878)
                     /locus_tag="VC394_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002275264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEP5994033.1"
                     /translation="MKERVVWGGVAVAIFLPFLIIGNLPFQLFVGVLAMIGVSELLKM
                     KRLEVFSFEGVFAMLAAFVLAVPMDHYLTFLPIDANVAFYSLMVFFILAGTVLNSRAY
                     SFDDAAFPIATSFYVGIGFQHLINARLSGIDKVFLALFIVWATDIGAYLIGRQFGRRK
                     LLPTVSPNKTIEGSLGGIACAVLVSFIFMVIDRSVYAPHHFLTMLVLVALFSIFAQFG
                     DLVESALKRHFGVKDSGKLIPGHGGILDRFDSMIFVFPIMHLFGLF"
     gene            complement(38891..39640)
                     /locus_tag="VC394_000540"
     CDS             complement(38891..39640)
                     /locus_tag="VC394_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEP5994034.1"
                     /translation="MFGLKAKSTKKVLGSIPKHIGIIMDGNGRWAKKRLKPRVFGHKA
                     GMDALQEVTITASELGVKVLTVYAFSTENWSRPQDEVSFIMNLPVTFFDKYVPVLHEN
                     NVKIQMIGETSRLPEDTLAALNAAIDKTKRNTGLILNFALNYGGRAEITSAVRFIAQD
                     VLDAKLNPGDITEDLIANYLMTDHLPYLYRDPDLIIRTSGELRLSNFLPWQSAYSEFY
                     FTPVLWPDFKKAELLKAIADYNRRQRRFGKV"
     gene            complement(39859..40224)
                     /gene="yajC"
                     /locus_tag="VC394_000541"
     CDS             complement(39859..40224)
                     /gene="yajC"
                     /locus_tag="VC394_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEP5994035.1"
                     /translation="MGFPTILMFVVMLGLIWFMQRQQKKQAQERQNQLNAIEKGDEVV
                     TIGGMFAIVDEVDTTAKKIVLDVDGVFLTFELSAIKRIVTKATTETTLVETEETSVVN
                     EPAVETVDSTDTDSAIESH"
     gene            complement(40340..40714)
                     /locus_tag="VC394_000542"
     CDS             complement(40340..40714)
                     /locus_tag="VC394_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HEP5994036.1"
                     /translation="MRPKEKETSMTFEEIVANFIPSSVAEVTSAIASGKDMIVFLGRS
                     SCPYCRRFAPKLAQVATDNQKEVYFVDSENAADAAELAAFRENYQLVTVPALLVSYDQ
                     HQRAVCDSSLTPDDILAFLTRE"
     gene            complement(40829..44326)
                     /locus_tag="VC394_000543"
     CDS             complement(40829..44326)
                     /locus_tag="VC394_000543"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase"
                     /protein_id="HEP5994037.1"
                     /translation="MKKKVNQGSKRYQYLLKKWGIGFVIAATGTVVLGCTPSILTHQV
                     AAKTIVGLARDEAQQGDGNAKSGDGLQSSSKEAKPVLDSSLANPASIAEHHLRMHFKT
                     LPAGESLGSLGLWVWGDVDQPSKDWPNGAITMTKAKKDDYGYYLDVPLAAKHRQQVSY
                     LINNKAGENLSKDQHISLLTPKMNEVWIDENYHAHAYRPLKKGYLRINYHNQSGHYDN
                     LAVWTFKDVKTPTTDWPNGLDLSHKGPYGAYVDVPLKEGANEIGFLILDKSKTGDAIK
                     VQPKDYLFKELDNHTQIFVKDTDPKVYNNPYYIDQVSLKGAEQTTPNEIKAIFTTLDG
                     LDEDAVKQNIKITDKAGKTVAIDELTLDRDKSVMTLKGDFKAQGAVYTVTFGEVSQVA
                     RQSWQLKDKLYAYDGELGATLAKDGSVDLALWSPSADTVKVVVYDKQDQTRVVGQADL
                     TKSDKGVWRAHLTSDSIKGISDYTGYYYLYEITRGQEKVMVLDPYAKSLAAWNDATAT
                     DDIKTAKAAFIDPSKLGPTGLDFAKINNFKKREDAIIYEAHVRDFTSDKALEGKLTHP
                     FGTFSAFVEQLDYLKDLGVTHVQLLPVLSYFYANELDKSRSTAYTSSDNNYNWGYDPQ
                     HYFALSGMYSVNPNDPALRIAELKNLVNEIHKRGMGVIFDVVYNHTARTYLFEDLEPN
                     YYHFMNADGTARESFGGGRLGTTHAMSRRILVDSIAYLTREFKVDGFRFDMMGDHDAA
                     AIEQAFKAAKAINPNTIMIGEGWRTYQGDEGKKEIAADQDWMKATNTVGVFSDDIRNT
                     LKSGFPNEGTAAFITGGAKNLEGLFKTIKAQPSNFEADAPGDVVQYIAAHDNLTLHDV
                     IAKSINKDPKVAEEEIHKRIRLGNTMILTAQGTAFIHSGQEYGRTKQLLNPDYRTKVS
                     DDKVPNKATLIDAVAKYPYFIHDSYDSSDAVNHFDWAKATDSIAHPISNQTKAYTQGL
                     IALRRSTDAFTKATKAEVDRDVTLITQAGQDGIQQEDLIMGYQTVASNGDRYAVFVNA
                     DNKTRKVVLPQAYRYLLGAQVLADAEQAGVTAIAKPKGVQFTKEGLTIDGLTALVLKV
                     SSKTANPSQQKSQTDNHQTKTPDGSKDLDKSLMTRPKRAKTNQKLPKTGEASSKGLLA
                     AGVALLLLAISLLMKRQKD"
     gene            complement(44524..46137)
                     /locus_tag="VC394_000544"
     CDS             complement(44524..46137)
                     /locus_tag="VC394_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEP5994038.1"
                     /translation="MKKHWWHKATIYQIYPRSFKDTSGNGIGDLKGITSQLDYLQKLG
                     ITAIWLSPVYQSPMDDNGYDISDYEAIADVFGDMADMDELLAAANERGIKIIMDLVVN
                     HTSDEHAWFVEARENPNSPERDFYIWRDEPNDLTSIFSGSAWEYDNASGQYYLHLFSK
                     RQPDLNWENEALRHKIYDMMNFWIAKGIGGFRMDVIDLIGKVPDSEITGNGPRLHDYL
                     KEMNQATFGNHDVMTVGETWGATPEIARQYSRPENKELSMVFQFEHVGLQHKPDAPKW
                     DYAEELDVPALKTIFSKWQTELKLGEGWNSLFWNNHDLPRVLSIWGNDSTYREKSAKA
                     LAILLHLMRGTPYIYQGEEIGMTNYPFKDLTEVNDIESLNYAKEAMGNGVSAARVMDS
                     IRKVGRDNARTPMQWSKDTHAGFSEAKETWLPVNPNYQDINVADALADPDSIFYTYQK
                     LIALRKEQDWLVEADYHLLPTADKVFAYQRQLGEETYVIVVNVSDEEQVFAKDLTGAQ
                     VIITNTDVDTVFETKHLQPWDAFCLKLKA"
     gene            complement(46266..47399)
                     /gene="ugpC"
                     /locus_tag="VC394_000545"
     CDS             complement(46266..47399)
                     /gene="ugpC"
                     /locus_tag="VC394_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEP5994039.1"
                     /translation="MVELNLNHIYKKYPNTTHYAVEDFDLDIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELKIGGEVVNDKSPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYKKDDIDRRVKEAAQILGLTEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGSTTIYVTHDQTEAMTLADRIVIMSATKNPQGN
                     GTIGKIEQVGSPQELYNLPANKFVAGFIGSPAMNFFEVEVKDGRIVSEDGLAIAIPEG
                     QAKMLEAAGYKGKKVTFGIRPEDISSRQIVHDTYPSATVTAEVLVSELLGSETMLYVK
                     LGQTEFASRVDARDFHSPGEQVSLTFNVAKGHFFDRDTEQAIR"
     gene            complement(47697..48545)
                     /locus_tag="VC394_000546"
     CDS             complement(47697..48545)
                     /locus_tag="VC394_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEP5994040.1"
                     /translation="MELKDYFPEMQVGPHPLGDKEWVSVKEGDQYVHFPKSCLSEKER
                     LLLEVGLGQCEVLQPLGSPWQRYLLDHQGNPPQLFETSQFIYLNHQQALPDDLVELLQ
                     QMIAGLEVILPISATQTAFLCRQAISIKVLRWLEDLLPTLESDFGLALTMFVGNAWYQ
                     VAAGTLRECFEEECQLLTAYLRQQSGRKLLTFSGLMLWSLLSHHTFLALTRQFHQSLS
                     PQSDMADVVHALWSEHGNLVQTAQRLYIHRNSLQYKLDKFAQQSGLHLKQLDDLAFAH
                     LFLLKY"
     gene            48885..50207
                     /gene="ska"
                     /locus_tag="VC394_000547"
     CDS             48885..50207
                     /gene="ska"
                     /locus_tag="VC394_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023611186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptokinase"
                     /protein_id="HEP5994041.1"
                     /translation="MKNYLSFGMFALLFALTFGTVKPVQAIAGPEWLLDRPSVNNSQL
                     VVSVAGTVEGTNQEISLKFFEIDLTSQPAHGGKTEQGLSPKSKPFATNSSAMPHKLEK
                     ADLLKAIQEQLIANVHSNDGYFEVIDFASDATITDRNGKVYFADRDDSVTLPTQPVQE
                     FLLSGHVRVRPYQPKAVHNSAERVNVNYEVSFVSETGNLDFTPSLKEQYHLTTLAVGD
                     SLSSQELAAIAQFILSKKHPDYIITKRDSSIVTHDNDIFRTILPMDQEFTYHIKDREQ
                     AYGINKKSGQEEKMNNTDLISEKYYILKKGEKPYDPFDRSHLKLFTINYVDVNTNKLL
                     KSEQLLTASEHNLDFRDLYDPRDKAKLLYNNLDAFGIMDYTLTGKVEDNHNDTNRIIT
                     VYMGKRPEGENASYHLAYDKDRYTEEEREVYSYLRYTGTPIPDNPNDK"
     gene            complement(50305..50748)
                     /locus_tag="VC394_000548"
     CDS             complement(50305..50748)
                     /locus_tag="VC394_000548"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEP5994042.1"
                     /translation="MKLVLQRVKEASVSIDGKIAGAINQGLLLLVGVGPDDNAEDLAY
                     AVRKIVNMRIFSDADGKMNQSIQDIKGSILSVSQFTLYADTKKGNRPAFTGAAKPDLA
                     SQLYDSFNEQLAEFVPVERGVFGADMQVSLINDGPVTIILDTKCH"
     gene            complement(50763..52982)
                     /locus_tag="VC394_000549"
     CDS             complement(50763..52982)
                     /locus_tag="VC394_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEP5994043.1"
                     /translation="MAKIMNVTGEEVIALAATYMTKTDVAFVAKALAYATAAHFYQVR
                     KSGEPYIVHPIQVAGILADLHLDAVTVACGFLHDVVEDTDITLDEIEADFGQDARDIV
                     DGVTKLGKVEYKSHEEQLAENHRKMLMAMSKDIRVILVKLADRLHNMRTLKHLRKDKQ
                     ERISRETMEIYAPLAHRLGISRIKWELEDLAFRYLNETEFYKISHMMKEKRREREALV
                     EAIVSKVKTYTTQQGLFGDVYGRPKHIYSIYRKMRDKKKRFDQIFDLIAIRCVMETQS
                     DVYAMVGYIHELWRPMPGRFKDYIAAPKANGYQSIHTTVYGPKGPIEIQIRTKDMHQV
                     AEYGVAAHWAYKKGVRGKVNQAEQAVGMNWIKELVELQDASNGDAVDFVDSVKEDIFS
                     ERIYVFTPTGAVQELPKESGPIDFAYAIHTQIGEKATGAKVNGRMVPLTAKLKTGDVV
                     EIITNANSFGPSRDWVKLVKTNKARNKIRQFFKNQDKELSVNKGRDLLVSYFQEQGYV
                     ANKYLDKKRIEAILPKVSVKSEESLYAAVGFGDISPISVFNKLTEKERREEERAKAKA
                     EAEELVKGGEVKHENKDVLKVRSENGVIIQGASGLLMRIAKCCNPVPGDPIDGYITKG
                     RGIAIHRSDCHNIKSQDGYQERLIEVEWDLDNSSKDYQAEIDIYGLNRSGLLNDVLQI
                     LSNSTKSISTVNAQPTKDMKFANIHVSFGIPNLMHLTTVVEKIKAVPDVYSVKRTNG"
     gene            complement(53234..53344)
                     /locus_tag="VC394_000550"
                     /pseudo
     CDS             complement(53234..53344)
                     /locus_tag="VC394_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_129544913.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
BASE COUNT        15846 a        11711 c         9888 g        16078 t
ORIGIN      
        1 cgtcttcatt ttcaatgagt aaaaagtgca aaaatgactt ttttcatatt ttttagtaag
       61 atttttgaga tgtcacataa gaggtcactt tataacaaat agccctctga tataaaaaac
      121 aaaactcgtt ctttacagct attttacaat ctaatcgctt cttgttataa catcaatctc
      181 taatcagaaa atggttactt tacttattta atcaggtcag gagtttgaat gacagaagtc
      241 aaatatctga aaaaggatat taaataaaac tttcaaaacc cattttcatc aactaaaaac
      301 acttataacc cgatcttaca actaatggat taccatcaac agcaaataat actatgcttg
      361 aaaaatgtca acatcatcta ctttttgtga ctataattca gtaaacgaac atgaagtgta
      421 aaaatgtatt aaaaaagtat ctcaaaatca ttcaatcact tgagtttctg ctactttttt
      481 ataccagtag gcactcttct taggataacg ttcttgagtt tcaaaatcga catagaagag
      541 accgtaacgt ttttcatagc catttgacca tgaaaagaca tccatcagtg accacataaa
      601 gtatccttta acatttgcac catcagaaat agcatcagaa ataacttcta agtgtttttt
      661 cacataatcg atacgtccat catcatagac agtattatct acaaactcat ctttgtagcc
      721 aagaccattc tctgtaatgt aaattttctt gtagttagga taatcggctt tgacacgcat
      781 gatttgatca tataagcctt gtgggaagat aatccagtcc cagtccgttt ttggaacatc
      841 aacgggtgct tttcttcgac caacaccctt gatttggtat ttagagctgc ctttttcccc
      901 tttaccatta tgaatgattt cagtctcacc atcaaaagct tgcatccaat cactcatata
      961 atagttaata ccaaggaagt cattcaaatc ttttgcagcg tctagtgcag caaaatcttc
     1021 ctcacgaaga tcaagttcac caccatttac ctcaaggata tggttaacac cttccattgt
     1081 tttatctgaa tacttaccta gataagttgc atcaagaata aatttattat ggatgatatc
     1141 ttccagttca gctgctctaa catcatcagg attgttagcg tcaaatggat acttagttgg
     1201 aagagcatgg accacgccga tttcacctga atagccacta tctttaaaga gtttgactgc
     1261 acgagcatga gagaccatca tgttatggtg tgattggaaa actttagcaa gatcatattg
     1321 gatacctgga gggaatttac caaccaagta ttggccatca ccaataggcc caatttcgtt
     1381 aaatgttgtc caatagttaa cttctgagaa ttctttaaaa caaaattctg cataatttac
     1441 aaaatgttca atgttctcac gattgaggaa atcaccatcc gagtggagag cttctggggt
     1501 atcaaaatgg tgaagtgtaa caaaaggctc aacatgacgc ttatgacact ctgcaaagag
     1561 attgtggtag tattcaactc ctttagggtt aacttctcct tttcctgttg gaaaaatacg
     1621 agaccaggca atagagatac ggatgccgtt gacaccaaat tcttcactaa gtttcaaatc
     1681 gactggataa cgattgtaaa aatcacttgc tggctcagct gtgtaccaat agttgtcttc
     1741 taagtattta tcccaagcta ctggtccttt accatctgtg tgggtagcgc cttcagcctg
     1801 gtaagcagct gtagcaccac caaaaataaa atctttaggt aatgttttag tcatgatata
     1861 cctcttctaa attttcattt cactataaag gaagattgag tacccccaac cttccctagc
     1921 gacatattat ttttccattt gttctttaac gaagtctaat gcgccttgac catcgcgagt
     1981 caatttgatg tattgagccc cttctgtttt agctaattta atatcaagtt tatcagtttc
     2041 cactttcata tcttcatagt tagaagctac ttgaggtgca aggataacaa gttgatattg
     2101 tggtaaaatc tcacggtgag caccataact tcctgctgct gcagtgacag gtgcaccata
     2161 ttctttagct gctttattca aagcgtttgc aagcaaacca cttgtaccac caccggcaca
     2221 aagaacgaga acattagttt gatcagtgat ttctttggtt tctttagctg ctgatttttc
     2281 aagaatagca tcagcttttt tagtatcaaa gtttgctgct actttttctt tgagggcatc
     2341 tgatgaattc gtacctgctg cttcttcagc aagaatttgc tcatcatata cttttaagaa
     2401 tgggtagtag ataatcacat caacaacaat taacaaagct gccaaaatga atgctagcac
     2461 ttggaagttt gtcccaagga taagacccaa tggagctggt gttgtccaag gtaagtttgc
     2521 agtgaaactg ttcattccaa gtgtgtcaat gaagaatttg aaaatccata cgttgacaat
     2581 cggtgccagg ataaatggaa taaagaagat tgggttcaaa acaagtgggg caccaaacaa
     2641 aatgggttcg ttaacaccaa agaatgttgg aacaactgat gcacgtccaa tggctttgtt
     2701 acgttttgat tttgttaacc acatgaacat gaatggaacg acgagagttg caccagtacc
     2761 acccatggta acgatgaaca tttgagtccc tgaagtcaag atcttatcag catgttcacc
     2821 agcttgaagt aattgaaggt tggtttcagc atttgcgtaa gtaatcgctg caatggctgg
     2881 ttcaacaata gatggtccat gaataccaac aaaccagaag aaagcaaatg caccgaagat
     2941 aatagtgatt ccaacatagc catctgctgc ttggaacaat ggtgcaagaa gtttaccaat
     3001 tgattctgca acgttaacgc ctaaagtagt atgaacaatc aattcaatca cataaagcag
     3061 tacaactgat aacgtaaatg gaatcaaatc tttaaaaact tgagatatat ttggtggaac
     3121 ctcttctggc atgcgaatag taacattgtt tttcacacag gttttataga catttacggt
     3181 aataaaagct gcgatgaagg ctgtcaacaa accttttgtt cccataaatc cagttaagaa
     3241 accaccgtct tttgctggat tggctgccat aagtaaaaaa cctacaatag cagcgagcat
     3301 agttgaaata aagttgattt ggtttgtgct tggtaatgat cgattcattg agtcagttaa
     3361 agctttggct gtcgtaccag ccacaaacaa acctaaaata cccattgagt agttgtatgg
     3421 cgtcatcaag agattctcaa tatccttact ccaatggaag ccccatgcat ttggaacata
     3481 ggcaatcaag ataaagatac ttgaaaacaa gataacttgc ataccagcga taaaaccatc
     3541 acgaatagcg cgaagataga tatttcgaga aattttctcg aagaaaggtt tccctttctc
     3601 aatctgagca attaatccat tcattatttt gctcctctct tataaagttc aatcatgtgt
     3661 ttcattaaat cttgtaataa aatggtcgtc attaaatgat cttgaccatg cattaaagtg
     3721 acactataag ctaagtcatc tccagctgct tctttttgta gtagacttgt ctgagcattg
     3781 tgagcatcat tcaagcagtc actacctgca gcgattaatt cttctgcttt agcataatct
     3841 ccttcttgcg ctgctttaag cgcttccaaa aattttgaac gggcatcacc agcataggct
     3901 acgatttcaa aacctaaaag agtagtttct tctctattca ttgtagtttc ctccgaaaat
     3961 aatgttacat tttttcagtc caaggacttg cagttttatc tagtactttg tttaattcgt
     4021 caatattttt gaaaccttct gtacgcaacc attcacgagc tgcttgtggt ccttcttcga
     4081 tatagacttt tacagatcct gcccaggtag cacgtccaca taagacaccg ttgaatttag
     4141 cacctgattc tgctgcaaac accaaagtat cttggaacaa tttggcagaa acacctgcac
     4201 tcaagtaaat atatggtaaa tcagtagaag cttcttggtc acggaaagct tgagctgctt
     4261 cttcttttgt aaagagaacc tcaccatctg caaagctttc tacaaatttc atatttactg
     4321 gaacttcgac tttcaagacg tcaacaccaa agcgttcttc tgagaagact ttcattgctt
     4381 cattaacttt atgagcttta acttttgcaa actcagggct tgcattgtca gcaattttct
     4441 catcataagt tagaatttct aagtagaatg gaatatcttc tgcacggcat tcagaaccaa
     4501 tacgttcaat gtaagctttt ttctgctcgt taacatcttg atctccatca atatcatagt
     4561 aaagaaggaa tttaactgct tctgctccag cttctttaat acgttttgca gaccaaacat
     4621 ctaagcaatc aggtaagcga ctagttgttg ttgcatcgta tcctgttttc tcatatgcca
     4681 acaacaagcc tgcttcttct gaacgaacac gacttgctgg caagccatat tcaggatcaa
     4741 gtaaaattga tgatgcaaat ggtgtcagtt cttcagaaac taaactttta agctcttcaa
     4801 tttgttctac tgttggctct tttgtttgat gttgagccat catacgtttg agagctccac
     4861 gttggtcaaa agctaaagca gaaataacac catctatact tagtttttcc atgcttttac
     4921 gtttgttttc agttaaagta attgtcattt ttatacctct ttgactttaa tagattgata
     4981 taacttatcg taatttgcca tgttaacatg cccagttgtt ttttcttgcg catttaacat
     5041 acctaagaca tttgcttttg ttaataaagc tcgatcatct tcatcattcg ctaaacctga
     5101 agcaatacct gccactgtag aatcacccga acctacagcg cttacaattt tgattttagg
     5161 aatgtcaacg ttataaaacg tatccttgtg ctttgcaaat acaccatcag caccaagaga
     5221 aacgattatc cattcaattc cttcaaatag ttcatcttgt aagacttctt ttaaggcttc
     5281 aaaatctttt gtcatttctt tacctagaag ttgagaaagt tcttcaagat taggtttgat
     5341 aactgttggt ttactatcac ctttcaatac tgcttctaaa gcatttccag aacaatccag
     5401 aacagtcttc ttaccattaa gattggcaat cttgattaac ttctgatagt aatcatctgg
     5461 cataccagca ggcaaactgc ctgagagcgt cactacgtca acatcattca gtaaatattt
     5521 aaaatgatga gtaaatccat caatttcatc tacacttaac agcggtcctt tttctaaaat
     5581 ctcagtttga ttaccttcgt gtaatatggc tatacatgtt ctggtatcac ctgaaatttt
     5641 atagaagcga cttaaaatcg aatcaggaag atgattaatg ataaaatcac cgctttctcc
     5701 accaatacaa ccagtagctt tgactgtctc accaaagtca tttagaactc gggaaacatt
     5761 caagccttta ccaccgggtg tctttgtaac gtctacaact cgattgacag tatcgcattt
     5821 tagctcattc aaagggtagg agacgtcaat agctggattt aaagtaactg ttaaaatcac
     5881 aatagctcct ttttctatat atccttatca taattctttc aaatattagt cgtgatattc
     5941 accacggtcc catttttcta gaaattcatc aaagaagtgt gggttgtttt gatcttggct
     6001 agcatgttgc aagctatcca ttttttcaat ccatttttta ttttcttcag ttggtttgta
     6061 ttcagccgct aagaaagcat cgataatatc attcatcagc aaaccaccga tgatttttcc
     6121 accaaaacca ataacgtttg cgtttagctc ttcttttgag tagatagcag atgtcatatc
     6181 tctaacgagt gctgcacgaa taccgggaac tttgtttaca gcatttgtga taccaacacc
     6241 agtaccacag ataacaacac ctaattctgc ttcaccactt gctactgctt caccaacttt
     6301 tttaccaaag attggatagt gtgtgcgaac attatcgtat gtaccgcaat caatgatttc
     6361 atgaccttgt gattttaggt aatccacaac agcgattttt tcataagtaa caatatggtc
     6421 acaacctaca gcaattttca tttttgttct ccttgtttta acctatttca atcaattagc
     6481 acattttatt aagcatgtcg acacgaactt gatgacgtcc accatcatat ttaccttcga
     6541 caaagccttt gataatgttc tttgcaattc ctggagcact aatttcagaa ccaagcgtga
     6601 tgattcgagc attgttgtgt cctcttgtca tgtatgctga acgctcatct gatacttcag
     6661 ctgccaccat tcctttgatt ttagtagcta ccatgaaaga accaacacca tatgcatcaa
     6721 tgacaatacc taagttatct tcggaggcat taacttcttt tgcaacggct aaagttgtat
     6781 ctacaaagtc acgtacttcg ttttcactaa catcaacgac ttcgtattta ccttctttaa
     6841 ggtaatcttt gataacttct ttaagttctt gacctgcttt gtcagcaccg ataataattg
     6901 ccattttatt tctcctatga tttagaataa gtaaatgtgg aaaacctacg tagatagtta
     6961 attaacgatt tgcaacatct tttgaaataa cgctttcatt atttctatga cctaatcata
     7021 cacccaccaa acataaaagt caacattttt ttgtttattt ttataaaaaa taaacaaaat
     7081 atgtttattt ttttcatttt tacgttatta tcctaatgtt tcttgtgttt ctttttcatt
     7141 tcataaatcc ctttgacagc cactcgtaac ctatcatata ttacgagacc cttatctcta
     7201 tttattgtaa acgttttatt taaaacattt tctattttgt taatttttgt tttttccaat
     7261 gtttatgttt gttttgtttg atttttttat tgatattatg ttatgatacc tttaatagtt
     7321 ataccctaag acactgatag gagataagaa tgaaaaagaa agagcgtcat gaaaaaattc
     7381 tcgatatctt aaaagttgac ggttttataa aagtaaaaga tattattgat gaaatgaaca
     7441 tctcagatat gaccgctaga cgagatctcg atacattggc agataaagga ttacttatca
     7501 ggacccatgg tggtgctcaa tatttggatt attcttcagc aaaggacgaa ggtcacgaga
     7561 aaacacacac tgaaaagaag gtcttacaga ctaccgaaaa aaaacaaatt gcacaaagag
     7621 ctaaaaacat tattaaagac ggcgaaactg tctttatagg tccagggaca acattagagc
     7681 atctagctgt cgaattaaaa aatagaaata tcagggtcat tacaaatagc cttcctgttt
     7741 ttttaatttt gcacccgagc actactattg atttgatttt aattggtggt gaatgtcgtg
     7801 aaattactgg agcttttgtt ggtgctattg ccattaataa tctagaatca ttaacttttt
     7861 caaaggcttt tgttagcgca aatgcagttt acaataattc tattgcgacg tacagtgatc
     7921 tcgaaggaga agttcaaagg gttgcattga ataatgctgt tgagaaaatc cttctagttg
     7981 atagtaccaa gtttgaaagt tatgacttct ataattttta ccaactagat caagtggact
     8041 taattgtgac tgataaaaat gtgacaactg atatcataga aaagtacaag aaattctcaa
     8101 aaatcataat cgctgacaag taaaggacct actcatatta aaaagcactt cagggtttag
     8161 atatatcccc tgtcaagtaa acagggagca tatctgaaag tagtgtttgt gttataaaga
     8221 agtttcttgc taacttattt tctggttttt ttcagactta tcagtcttgt actgttcctt
     8281 tttctgatga ataacgtgga aataggcaga cagctatata tttcacctct agaatggttg
     8341 tcattactgt catcaagaca tgcttgttct ttatcgtatg gagtgaagct ttcttttaga
     8401 catcaacata cctcttaagt agattttggt tcgttaccat gtctacttaa gaggtgtctt
     8461 caaagcgctt taaaacgttg atataacagt atattaacgt tttgagaatt gtgaagcttt
     8521 acgagctttt ttaagacctg gtttttggtt cactccatta tttaaagtca aatcccttta
     8581 aaatcaatgt ttctaaaatg aaaaatcact aaaaatctta tttatagaca aatatttaaa
     8641 cctaaacggt atcaaaaata agtttcggag cttatcaaat aatgaaaatc tgtctcaatt
     8701 accgagtctt ttttattgac ttttttgtat atatgatata attaaataaa ttacataaag
     8761 gggttacata aaatgactac agtaaaaaaa acagagcggt tacctttcaa gctaataaag
     8821 aattggtaag cgaagcaatg acagtattaa acaaaaaaaa tttaacctta tcatctgctt
     8881 taagattatt cctacaaaat gtcgttgtca caaacgaggt tgacttattg acggaagagg
     8941 agttagagaa agaaaaactt ttcaagcaat ttcaagctga aatcaacaaa aatattgagg
     9001 atgttcgtca agggaagttt tatacctctg aagaagtgag ggctgaactt ggactataag
     9061 aaatatcaga ttatctatgc tcctgatgtt ttagagaagc taaaagaaat tcgtgattat
     9121 atttctcaaa actattcctc aacatcaggt cagcgtaaaa tggagcaaat cattagcgac
     9181 atagaaaaac tcgaggtttt ccgagaagtt ggttttgatg ccgatgaaaa atatggttca
     9241 aaaatcatcc attatcacag cactaaaggt tataccttaa gtaaagatta tattgtccta
     9301 taccatatcg agggggaaga aaatagggtc gttattgatt acttgcttcc tactcaaagc
     9361 gactatataa agttattcaa ataacacatt aaaagcctta gcagtcttgc tagggctttt
     9421 ttggtagatt accaattaac taggagcaaa ataagttcgg gctgtttagt agatattcca
     9481 caaagaagcc aaagactacg aagataggct aatctacctt aaggcttcag gaaacttaga
     9541 ggagttcacc aagccaactc ataaaactta tagcgagata ttcgaggaat ggtatcaagc
     9601 ctatcaagat acggtagagc caaccacggc ttctcgtact cttgatctgt ttcaacttca
     9661 cattttgcca gtcatgggag aattgcccat taacaaaacc tcccctttag actgccagaa
     9721 cttcatcacc gataaagcaa agacctttaa gaatatcaag caaatcaagt cctacactga
     9781 aaagatcttt gattttgcca tcaagatgaa actgctcaag cataacccta tggctgaaat
     9841 cattatgcca aagcggaaaa agacacgaat agaaaactat tggacggttc aagaacttca
     9901 ggagttttta gccatagtac ttcagaaaga gccttacaag cactacgcac tatttcggct
     9961 actagcttat agcggcctta aggaaaggcg agctatgcgc tcttaaatgg gctaatatag
    10021 actttcagag tgagacacta agcgtcaata agagcctagg acgattagac ggacaggcaa
    10081 tcgaaaaaag cactaaaaat gatttctcag taagaaagat taagctagac agcgaaacta
    10141 tttctattct gcaagaatgg aaagccatca gccaaaaaga aaaggcacaa ctagcagttg
    10201 cacctctttc aattgagtct gactttatat ttacatactg tactagagaa gggagtattg
    10261 aaccacttca tacagactat attaacaata tcttatcaaa ggtcattaag caacatagtc
    10321 ttaaaaaata agtcctcacg gcttcaggca tacccatgca accctgatga ttgagattgg
    10381 agttgaccca gtaaatacag ccaaaaggtt aggacatgct agtagtcaaa taactcttga
    10441 tacatacagt catgctactg cagtcggaga agacaaatca atcaaacaat ttgctaacta
    10501 tctcaaaaat aactaaataa acgtattaaa ccaagtcaaa agttttccga aacggtatca
    10561 aaaaaaggta tcaaaagcaa aaaacgacaa aaaaagcacc ttgcaagaga cttgcaaaat
    10621 gcttgtaatc gttgatgtaa agctgttctt aacgttttga gaattgtgaa gctttacgag
    10681 ctttcttaag acctggtttt ttacgttcaa ccatacgtgc gtcacgtgta agaaggccag
    10741 cgcgtttcaa cgaatcgcgg aagtctgggt ctacttgaag aagcgcacga gcgataccat
    10801 gacggatcgc acctgattgt ccaccgtaac caccaccaac aacgttaacg aaaacgtcgt
    10861 atgaaccttc agttgatgta actgcaaaag gttggttaat gatcaaacgt aggtcagcat
    10921 gtgggatgta ttcttctaca tcttttttgt taacagtgat tttaccagta cctggaacca
    10981 aacgaacgcg tgcaacagcg tttttacgac ggccagtacc tgcatattgt gcttgtgcca
    11041 ttatttaatg ctcctttcct cttagataag tcctgagatg tcaagtactt ctggttgttg
    11101 tgctgcgtga gtgtgctcac cgccaacgaa gactttcaat ttcatacctt gtgcacgtcc
    11161 aagagtgtta tgtggaagca tgcctttaac tgatttttca atcaagcgaa ctgcgttttt
    11221 agagcgaagt tcaccagcag tgattgattt caaaccacct gggtacattg agtgagtgta
    11281 ataaacttta tcagtcgctt ttttaccagt taatttaact ttttcagcgt tgataacgat
    11341 aacaaaatca cctgtatcag tatgtggtgt gaaagttggt ttgttttttc cgcgaagtac
    11401 gctagcaact actgcagaaa gacgtccaag tggcacatca gttgcgtcaa caacgtacca
    11461 tttgcgttca acttggcctg gtttagccat gaaagttgtt ttgttcatga tttctcctat
    11521 acgaattcgt aatatttgtt tacaaggtga tgggtgttcc atcccatcga aggtatttgg
    11581 aaggttccgg ggcctttcaa atggggtaaa caataccgcc tactatgata tcaaacttaa
    11641 gaggctccag tcaagtgctt tagctgattt tattggatta ttttttcttt cgtttttgag
    11701 gaatggcaga gctgctctgc tgatgttaat gggaatcatc aacctcccaa aaaaggtcat
    11761 ctaaggtctt atcaagtact cgacaaatgg caatgcaaag attaatcgtt gggttgtaat
    11821 cacctttttc aacagccgca atggtttgtc tggagacgcc taccaaatct gctagagctt
    11881 gttgagacaa atctttgcct gcacgcgctg cttttaattt gaggttcttc attcatctgc
    11941 ctctttagca tccagataat gacgatagaa aatactagct cccatactaa ggagcgttat
    12001 gcccaacacc attaaagatc cactgccgcc agccttgaaa aagcttccca ttttaagttg
    12061 ccctgccatc atctctctta ccgccaaacc tatagtgatt aaactcattc ctgttgctat
    12121 catccccacc agtatgcctt gttttccaaa gcgcctatcc acaaatggcg ataccccgtt
    12181 gaggttacaa tatgtcgttt gaactgccaa ccctccccaa agagccaatg taaagaggaa
    12241 gcttgcagat accagtctct ctgagaagcg atgaagaaag attgacagcc aaacagccac
    12301 aatcataaac caaaacccaa tagtgtatcc tttgagaaga atctgttttt ggcgttcgtc
    12361 atatctgggc agcttctgat cgtcccttac cttagaatct agtacttctt tgctatctag
    12421 tctcgtttgg agctttttcg ctgtctttag cccccatacc aacaatatga gaaagctagt
    12481 caatataacc gctacgcctc caactaagta acgttgaaaa ctggtcttag ccagtaatgt
    12541 tgctactatc acaactagat ccatcactgc tgttatgaca atgaaacccc attgtgactt
    12601 agcgagattt tgtttgctat caaacatgat tcgtttctcc tttaaatctt tatatgacat
    12661 aatataaatt attttggata ttttgtcaaa tatattttac attcttgatt gatttaagca
    12721 gcaaaaaagg attgaggatt tctcttcaat ccttataagg gattaacgag aattctgccc
    12781 aatcacaaaa actgccaggg tgttaggacc aacgtgagct gagataactg gtcctagtgg
    12841 catcataaga acatcgctaa tattctcatg cgccagcagc tcttctcgta acttttcggc
    12901 actaccctga tcgcttgtat aagagacaat aaccgtcgaa tccgcaatat ctttttccac
    12961 ttgagccacc atttctttga tggctttttg gcgtccccga atttttgcaa tgggcaccaa
    13021 tttcccttcc tcatcaatcc acaaaagagg tttgatactg gctaaactgc ctagaaaagc
    13081 tgaacctttt gacagacgcc caccacgcat caaatgaaaa agatcatcta ctaagaaata
    13141 ggtgcgcagt cgaggaatca ctgcttcaac aatatcttta gtctccagta aatttttgcc
    13201 actatctctg gcttctgctg ctaaaatggt tagatagcct tctcctcctg cagcagccaa
    13261 ggtatcaaca atttcaatca ctgcatcggg ataatcttct cgaacaagat cgcgtgccat
    13321 tagagcgctt tgataggtac ccgataaaac cgaagaaaaa gcaagataaa gcagtgcttt
    13381 gttgttccta gcatgttcac gaaaaacctt ctcaaactct cccacattaa tctgactggt
    13441 ttgaggatga ctgccagctt tcatcttttt caaaaggtaa tcactactaa tacggttagg
    13501 accgaccgtt tcataaacct ctccatcgca caaaatcgtt aatcctataa ggacaatatc
    13561 atgatcttct gcccaggttt gattcaaatc agcggttgaa tctgtcatta ttgtaaaggt
    13621 cattcttact tctcctcatc cagtttttta gtttgataat caaataatta ttgttttatt
    13681 ttatcaaaaa aacccgcact tggcgagtcg taattctcat tatcgatcta actgagacat
    13741 aaaatccttc aattgaataa gctgtccttg gtcaaaaggc cgcagtttag gcgttttgag
    13801 atcaatatct ctagacattt tatctaaatc agctttgaca gtatgaactc tttgctctaa
    13861 ttctcttgca gtgacacgaa gagctccttg agaaaaaatc gtccattgtt catttaaatc
    13921 actcgtagct acttctacca tatgtatagc cgtatttaac tctgctgcca tgcgctcaat
    13981 gtagctatct gctgtttcgt cttcctctgt aaataccact gagatataat actggtcata
    14041 tcgttgcctt aaccccggta catattgggc atcaaaaaca caaataatat taatattctc
    14101 aaaatgggca taatgattaa gttttgttaa aagtgtgtta cgtgcctgat caagctgatt
    14161 tatcttaaac aactgacggg ttgattgcca aaaggcaatc atattatacc catctactaa
    14221 taatatccgt tttttcataa tttagcgcct atttcggaaa acttcataca tgagaatagc
    14281 tgccgctacg ctagcattta agctctgtac gtgaccattc ataggaatcg taatcatttc
    14341 atcaacttgc tttttgatat tggtagagat gcctttgcct tcgttaccaa tgactaaagc
    14401 aagtttacca ttagtgttcc agcaatcaga cggtgttcca ttcatgtctg tgccaaaaat
    14461 ccagaatcct cttgctttca atttatctag agtttgacta aggttagtta ctctcgctat
    14521 cggaatatgt tcaacagcac ctgtagacgt ctttgagacc actggagtca caccaacaga
    14581 acgatgttta ggaataatca ctccacatac atttgtagca tcagctgtcc gtaaaatcga
    14641 tcctaaatta tgaggatctg tcagaccatc taaaataaga atcaaaggat ttgcttcttg
    14701 ctctgctatt tcgaggatct catcaacatc agtataggca aaagctgata ctctcagaac
    14761 aaaaccttga tgcacagctc catcagtcat ttgtgacaag gtttttttag gcgtccatga
    14821 aatagcgacc tttttttgtg tcgctaagct tttgatgtta tccactttct ttcctcttaa
    14881 atcctcttgg atataaagct tatttcctgt atttgcttga aggctttctg taacggcatg
    14941 aacaccatag acgatatcgt ttgtttcaat agtatcttta tcttccatga gtttattata
    15001 ccatgaatgc ttcaaaataa atgcatagac tcttttattt tcgtctagat actttttgaa
    15061 tagctataaa taaacctgca gctaaactga ctactagacc tgataagacc caagggaatc
    15121 ctctttttga agtaggtttt acttcctctt tgtccactag catttttgtc ttgttgctac
    15181 cgtttttttg gctattacta gcagttggtg taagtttctc atttttagta gatcgcttta
    15241 aggaagttga tcgatgatta gtagctgatt tgaaaggatt tgaagtagaa tctatttctt
    15301 ggttagctga ttgatttgtt tgagcttttg tgtctacatc gaccgaattc gtcgttgctt
    15361 tagaagtatc cttttgttgc atattagttg gtgtttttct agctttgttt tgatcaaaat
    15421 gaacagttcc tgattgacca gttactgcct tttcttgagt atgaacggtg acagttacat
    15481 gatagtcacc actcttttgt ccagttaaat caaaccaagc tgtaaaacga cgctgttgcg
    15541 ctgccatagt atatacccaa ctattaatag tattatcact agcgtcttta agtgttatcg
    15601 ttgcctgagt tggttgtctg acaggttgat ctgtggtgac tgtagcatca atataaggtt
    15661 ctgttccacg tatactggct tcaacactga cctgactaat agttgattca gctataacat
    15721 tatcagtgtg ctgatcttgt gcatacacag atatggcttt gccagaagtc cccagcaaac
    15781 caataagtaa agctagtgat aataagctaa ctgttttttg catagttgtc ataataaata
    15841 tctcctcttt aaattagtct taccctctca ttataaacta ttcgaaaatg atttcattat
    15901 ttttatgtct tttttgttac aaatgtatct tttttatatc attatgtctt tttgatgtca
    15961 taaaaagagt taatggctct ctacctttac ttcagaccta tgaaaataag agtctcctag
    16021 gggactctta tgaggatatc aattgttgct tttctacata ctcaatacac catctgatca
    16081 attcttctaa ccgctctttt tggcccatca tatcaagata acccataatg gcttcaaaac
    16141 ctgttgacat acgataggta ataatatcgg cattcttagc tttagtatgg ctattggtat
    16201 tgcgaccacg cttatagatg tcttcttctt tttcggtcaa tagctgcgct tctaacatag
    16261 cctgaatcaa gttggcttgt gccttagcag aaacatatct cgttgctaaa cggtgtagct
    16321 ggctaggttt cgttttacct tgaaaaatga gatgacgacg aacatagtag gaataaaccg
    16381 catctccttc aaaggctagg gcaatgccat taatcaaatt cacatcaact ggattaatca
    16441 cgcagccacc tcacaccatc tttggtatct agcagcttga tgccttgagc tgctaactga
    16501 tctcgaatgg catcagctat ggcaaaatca cgattcgcgc gtgcttcttg tcgcttagca
    16561 atcaaggctt caatgtcaac ctcaagcacc tcttcttcaa agataatgcc aaaaacagct
    16621 aacattttct caaaagcgct tttaacaggt tctgtatagg agccagaatt aatccactta
    16681 gccatgtcaa atacgacggt aatcccattt gctgtattga aatcatcgtc catagcgtct
    16741 tggaaagcta ttacaaactg ttttagctct tgctcatctg cagtttctgt cagcggttgt
    16801 tgcaaggtgt ttttaagata cttcagattg atttccgcat catgaatagc cttttcagtg
    16861 aagttaattg gttttcggta ctgttgtgtc gcaagaaaga atcgcaaaac ctgaccatca
    16921 acggtttgta acatgtcatg aacggtcaca aaattaccta gtgacttgga cattttttcg
    16981 ttatcaacag tgacaaaccc attgtgcatc caatagttag caaaagtctt gcctgtttta
    17041 gcctcagatt gggcaatttc attggtatga tgaggaaatt ccaaatcagc tccgccacca
    17101 tgaatatcaa tggtatcacc aagaatttca gtagccatca cagaacattc aatgtgccag
    17161 cctggacgac caaaccccca ggggctatcc caagaaacct cacctgcctt agctgatttc
    17221 caaagagcaa agtccagtgg attttctttt aaagctgtct cagcatctgt tcgaccactg
    17281 gctccaactt caagttctga cagggtttta ttagctagct tggcataatg ctctgacttt
    17341 tccacgcgga aatacacatc tccatctgct tcgtaagcaa aatctttttc aatgaggctt
    17401 tcaacaaatg aaatgatttc tgctatataa tccataacac gaggattttg tgtggctggc
    17461 ttaacaccaa gtgccttggt atcttctata aaagctgcaa taaagcgatc tgacaattct
    17521 ttaggagaaa cacctgcttg agtagcagcc ttgataattt tatcatcgac atcggtaaaa
    17581 ttggaaatgt aattgacctg atagccagta tactcaaaat aacgtcgaat ggtgtcaaaa
    17641 gcgaccgctg atctagcatt tccaatatga atataattat agaccgtcgg tccacaaacg
    17701 tacatattga ctgtattttc agtcaaaggt acaaacttgc ggagcgaacg ggtcatggta
    17761 tcataaattt taatcatgta aactccttat tcaatatcct agcattagtt gtcatcttga
    17821 tatcctaagt ctttttctga aatgctattc caccttttca acaatttaat attaataacc
    17881 aaacctatta accaaaatag ggtcaaaatg aagtatataa tcgctttaac catgtcttgt
    17941 ctcagtaaaa agattagact agccaccagt gtaatactag aaacaagaat agacaaactc
    18001 ataatgacat acagcccaac tcttatttta ttcatgaggc tcctttatta acgaaaaacc
    18061 ttcacaacct gtgaaggctt cagaaagtag acaaacccac ttttatgctc aaaaagcatg
    18121 aatagatctg ctgatttgac atcaggtttc ctatttctgc atgtggggtg agccaaaaca
    18181 gctgcttttt gttgtggtaa aagtggtatc aatactgaca gtgatgccca tacagatttt
    18241 tgagacctta ggctcaaaac taaggcatgg aattccaaag gaagtcgctg ccgtaagcac
    18301 cacctgagaa aaagtatcac aatagactgt ttcttcagtc taagacttgc ttagttgatg
    18361 ctcttttacg tgttttttgt attttgtaac attcccatat cactacactc agcaaccaaa
    18421 ctgatagggc tagctaagtt caacattact ctactagaaa gtcccaaaat attttatcgg
    18481 aagtcatcag tgcttctttt tgttattttc cgatcatttt gacaactgat aagagacctc
    18541 tcgttgtttt tgtaaacttt gaatttgacg attatctttt tgcccatgca ctcgtactat
    18601 cttagctggc acacctacaa ctgtcacgtc ttcaggaaca tctgataaca caacagctgc
    18661 tgcccctact ttagcatttg ctccgatgtc aataggtcca atcacttggg catgtgccga
    18721 aattaaggca ccttgtcgaa ccgttggatg acgcttgcca caatcttttc cggttccacc
    18781 tagggtcacc ccatgataaa gcatcacacc tttttcaaca attgctgtct ctccaataac
    18841 aagaccagca ccatggtcaa tgaagactcc aggagcgatt tgtgctccag gatggatttc
    18901 aatttgtgtc caaaatcgcc aaaattggct atgcattctg gctagtaatt taaaatgatg
    18961 tcgccaaaga aaatgagaca aacgatgagc agctagggct ttgatgcctg gataagttag
    19021 gataacctca agactattac gagcagctgg gtctagagct ttaacaatag ctatactttc
    19081 tttccaccaa cccatactaa ctcctacgac ttatctaatt ctgaccaatc aacaagaatt
    19141 tgttggtcat caaaaggatt gatggcgaca ccgaacgttt catcaagctc gttttcacca
    19201 tttcttggtt gataaatatc atcaatcgtc caagtcagaa tcactccttc agctttcctg
    19261 aaaaggcctc caaaatcgtc ttggttgtac catttcgcaa agctttgaag atttgaaaag
    19321 gcgggaacat agctttttcc ttcaggcgtt gacatggtag gaaaaaggcg gtcgtaatgg
    19381 ttattatctt taggataaac aaaggcagga acaaggtaaa ccttttccat cgtatctgcc
    19441 gccacattgt catcactcat aagcgtattt aacacagtgg tgtaatggtt cataaattga
    19501 atcatatcac tgcttttgaa aatggtagaa ttcccaaaat ctcccttttt cttaaggtta
    19561 aagacaagac ctgctgcacc agatgtaatg acttcttcta gcactgcaag agctgaacgc
    19621 tccagccaat actgactttg agcactctct tgttcttctt taaagcgagc catatcttcc
    19681 ttatcggtaa aaacaggggt aaccttgaca ccttcgactt ctatcacgta gggttcttta
    19741 gcagcccaga caggaaaatt atggaaagca ttgacaaggg caacactatc caaaaagtta
    19801 tcaggcgcat tgataaaagc tcttaagcga atatctaatt catttgattt agtcatgttt
    19861 ttcttctttt ttctctggtt ttggtgggcg tggaatcaaa gctttcattg aggcgtccac
    19921 acgacccttc tcatcaatct taatgacctt aacatcaacg tcttcaccca cttcaaggac
    19981 atctgacacg ttggtcgttc tagtccaagc aatttcagaa atgtgaacaa gtgcatctgt
    20041 cttgtcaaag aggttgacaa aggcaccaaa tttctcaata cggacaacct tagcatggta
    20101 aacttcgcct accttagctt cacgtactag gccagcgatg atttctttag tgcggttaat
    20161 ggcagcttgg tcacttgagt agatagagac atttccttcg tcatcaatat caattttgac
    20221 acccgtttcg gcaataatct tatcaatggt ttcgccacct ttaccgataa ccactttgat
    20281 tttgtctacg tcaatcttga tggtgtcaat tttcggtgca gttggggcta actcgggacg
    20341 tggttcagcg atagttgctt caatcacatc aaggatttca aagcgagctt ttttagcttg
    20401 tgcaagagct tcttctagga tttgaggcgt aatccctgca atcttaatat ccatttgaag
    20461 ggctgtaatc ccttcacgag ttcctgccac tttaaagtcc atatctccaa agtggtcttc
    20521 aagaccttgg atatcggtca agactgtata attcgtcccg tctgaaatga gacccatggc
    20581 aatccctgcc acaggagcct tgattggtac accaccagcc atcagagcaa gggtaccagc
    20641 acagatagaa gcctgagaag acgaaccgtt agattctaga acttcggcaa ctaaacgaat
    20701 ggcataagga aactcttcta agcttggcaa gacttgttca agagcgcgtt cacctaaggc
    20761 accgtgtcca atttcacgac gaccagcagc tccgtaacga cccgtttcac caactgaata
    20821 ttgcgggaaa ttgtagtgat gcaagaagcg ttttttgtac tctggggcca gaccatcaat
    20881 gatttgagtt tctcccattg gtgccaaggt caaaattgat agcgcctgag tttgaccgcg
    20941 cgtaaaaaga cctgatccat ggacctttgg caagaagtca accacagcat caagcggacg
    21001 aatttcgtca atcttacgac cgtcaggacg aatcttgtcc tctgtaatga ggcggcgcac
    21061 ttcagcatgc tccatttgtt caaggatttc tgccacgtca cgcataatgg ttgctaggtt
    21121 ttcgtcttct gcgtaacgct cttcgtactc agccttaacc atttctttaa cggcttctgt
    21181 ggctgcttca cgcgcttttt tctcttcaac ttgaacagct ttttgcagtt gagcattgta
    21241 cttagccaca atatcagctt gtaagtctgc atcaacctgt aacagttcaa cctcagcttt
    21301 ctctttacca acaactgcta cgatttgttc ttggaaggca attaattctt gaatggcttg
    21361 gtgacctttt aaaagagcct caagcatgat gtcttctgat aattctttgg caccagattc
    21421 aaccatgttg atggcttctt tgctaccagc aacggttaat tctaagagtg acgcttccat
    21481 ctgttcttta tctgggttaa tgatgaactc accatcaata tagccaactt gaacacctgc
    21541 aatcggtcca ttaaatggaa tatctgagat ggacaaggcc agagatgagc caaacatcgc
    21601 tgccattgga gcgctagcat tttcatcata agaaaggaca gtgttaatca cttgtacttc
    21661 gttacgaaaa ccctcggcaa acatgggacg aatcgggcgg tcaatcaagc gagctgtcaa
    21721 ggtcgcatct gttgaagggc gtccctcacg cttcatgaaa cctcctggga atttaccagc
    21781 ggcatacatt ttttcttcgt aattaacttg aagggggaag aaatcacccg ttgccatttt
    21841 cttagacata acagctgcgg tcaggacggt tgattctccg taacgaacaa cggttgcccc
    21901 gttggcttgc ttagcgactt gaccaacttc aacaacaagg ggtttccctg caaatgttgt
    21961 tgtaaaggtt tgttttgaca tatattctcc ttttaatttt cagaggggta aagaggtacc
    22021 tgttctctgc ttagattcag ttgttatacg ttgttttgct acaaaaagca acctgccacg
    22081 cttgtcacac gctcctcttt gtatcaaacg acgtatctgc tacattttac cataaaatag
    22141 cagaaatttc actgtcagat cgcaatctct tgaaatgtcc ttgaaaaata ttaaacgaaa
    22201 cttatactct ttaacaaagc aaaaaaagca ggttggtatg cctgctttcg tctcctaata
    22261 ttaatgattg tcatcctcta aaattttaaa gccgaggctg gtactgtctt tcagaaacat
    22321 ttgcacggtt tcaagggaat agtcttctaa atcttttcct tgaagaggca acttggcaat
    22381 caaatcagtg gtacaagtga taatgtccac gcctaactga tcagcttgat agatattata
    22441 agtttcacga ggactagccc acaacaattc gacctcttct ttttgacgac aaatgcgtag
    22501 agcttcttcc atgattggca ttgggtcaac gccggtgtct gcaatacgac cagcaaaaac
    22561 agacacaata gctggcactc cagcggttaa atgatcaacc acagcttgag tctgctcaat
    22621 tgtaaaaatg gctgtgacgt ttaacttaat gcctttggct gacaactttt gaataacggg
    22681 acaagttgat tcgcctgttg aagtggtgac tggaattttc acatagacat tgtcacctag
    22741 actggcaatt ttctcagctt ccttttccat gcttgcgaga tcatccgcaa agacttcaaa
    22801 ggacactggg tagtctgtaa ttttggcaag cacttctttg gcaaagccaa tataagaact
    22861 gatgcctgct tttttcatca aacttggatt ggttgtaaag cctgtgacca aacctgtttg
    22921 taaatccttt aacatggtct ctaagaccgc accgtctgaa aagactttaa cgtttagttt
    22981 ttttgtcatc atatcctcct tttagatttc ttctgaaaca ttattgatgg cttcttgaga
    23041 ttttgacaga aagtttggaa gcaccaatac aatagcaagc acagcaacca ccaagtaaat
    23101 accgatttga ccaatcgacg ttcctaagcg gcccagtaaa atccctaaaa caccgaaatc
    23161 cacatctcca aaggtcgtgt ttgaaaaacc aagtttacca agtacaggta gaagcatggc
    23221 tggcaagaag gcgagtaata agccattagc gaaagcacca atcattgcac cgcgacgacc
    23281 acctgttgaa tttccaaaaa tccctgctgt tgcaccgcag aagaaatgag gcaccatacc
    23341 tgggataatc aggacaccac ctgcaactcc taaaatcaac atcccaagta agccaccaac
    23401 gaaacttgac gcaaaaccga tgataacagc ggttggcgca taagggaaga ataccgcaca
    23461 atccacggct ggaatagcat ttggaattaa tttgttagca ataccttgga aggctggaat
    23521 caagtcagcc aaaatcatgc gaacaccggc gtagacaatt gccacaccaa ccgcaaaggt
    23581 aagaccactc ttaatagcaa agataaatgg gttttgacct gctgctaatt cttctgttac
    23641 tgaagtattc ctcaaaacag aagccactgt tgccaccaag tagaaaagca ccatgataag
    23701 tcctgttgaa atggtggtgt tgcgtaagaa actccatttt tcagaaatct gaaggtcctc
    23761 ggtatcttta ctgtctttgc caaccttgct accaacccag gcagaaaggt aatagcccaa
    23821 actaccaaag tgtcccatgg cgatttcatc tccgtcagtc actttcagag tatactgttg
    23881 accaatggct ggcgaaatag ctgaccaagc tcccaagagg aacccaccta agataatcaa
    23941 aagacttcct ttgaaatcaa cagctccaag cacggcggac aagagacaag ccataaagaa
    24001 gctgtgatgt cctgttaaga aaatgtattt gaagcgggta aaacgagcaa aagccaaatt
    24061 aagcaataac ccaaccacca agatggacat agtttccaca cccagaatct tttgagcgac
    24121 agaagtgacg gcttcattat ttggcactac gccagtgatg ctaaaaccgt gctcaatgag
    24181 tttggcaaga gggtccagat tggtcacaat aacacccgcc cctgcagcaa gcataaggta
    24241 tcccaaaatc ggccccaagg ttcctgtcaa caccttatga gcaggtgttt tcaaagccac
    24301 caaccctgca aaggcaatca agcccattaa aaatgcaggt tcttttaaaa tatctcgaat
    24361 aaatgataat aatgcttcca tagtatcttc ctatctcctt taaaggctta ttccttgcca
    24421 ctcatttcct tgagaaccgg aaccaaatct tcttcaattt ttttacgatt ggtatagctt
    24481 ttaacgatac aaaccttggc atcctctgga aaaatatctt taaattcttt aacggtaaca
    24541 aacaaatctg caccctttcc aacagccgca ttggaatcac aggattctgc atccacatca
    24601 atacccaaac tagaggcgat agcttctact ttcatgcgaa gtaacaagct actaccaata
    24661 ccgtttccac aaaccgttac aattttaatc atgtgattca tctccttttt caatcatatg
    24721 gctaatcaga ctcattattt cctcagtatt cttagcctca attaattgct caatatgttc
    24781 atcatcatct aaaatcaacg acagttcctg caaagccttc aaatgtgaac tagaatccac
    24841 agcagacaaa acaaaaatca attgcacttg cttgtctgga tcaccttctt gcttcaaatc
    24901 aaagctgaca ggttctttta gttgtaagag agacatgcct aactgccgcg ttcctttttc
    24961 aggcgccgca tgaggaacag ccaccttagg ggctaaaaca atataggctc caagctcatt
    25021 gaccgaatca atcataccgt cgatataaga tgtttcaatc atctggtgtt ctagtaaagg
    25081 ctgagcagcc aaacgaatcg cttcctgcca atccgaaaca gcatcagcct gcctaataaa
    25141 atctgctgtt aacaagtcca atagtcctag acctcctttt tcaatcgtta aatggttacc
    25201 aaacaggtaa cgggataaat cactaacaag tccttcttta ttagtaatcg tggtgtgttt
    25261 atgaataata ctcaacaggt catccaaagc gaattgctct gacaagggaa gatgaaagtc
    25321 atcacagacc atctttttca acatcatttt ttcaacaggt cccatcaaag cttgcgtcac
    25381 ataaacaggt ttaacacagt caaaagctac tgttgaaaaa atcaaatcaa aggaagctgg
    25441 atccaacaac tttattttgt ccagctggtg aatcctaata aactgtagct gtggaaaaag
    25501 ttccttcaag gttgcctcca acatcagaga cgaactgatg ccattagggc aaacagataa
    25561 ggctaccagc tgattgctcg gcctcttctt aggggcctgc aaccatctcc caaaatgaat
    25621 ggtaaaataa gcaatctcat cttcattaac tggtttcccc aattgttttt ctagaggaga
    25681 aaggctgcgc ttgaccaagt aaaagagaga ttcataatct tgtttgattt gttccttcaa
    25741 aggattggtc aagttaatgt cgtagtaaag tcggtaataa gctggcaaaa ggtgactgta
    25801 taggttctga cgaagttctg gggtatcggt gatagacaat cccgtgttaa ccgccaccga
    25861 attgatgatt tcctccataa tgtcataaat aggttgctga aagactaact ctaagtcacc
    25921 ttggatacaa ccaagtaacc tagattgaac caaatatttc tcttcggtta gactggggaa
    25981 gttgaccaat aactggtcca ccatcgtttc aacagctggc gaagctaaag gaaaagtatc
    26041 cactacttca gcccttacgt tccgtgaaaa tggtcgatga gcgagaaggc agaaaaaata
    26101 aatcaaatcc ctcaatttct ccgaaataaa agttaagtgg ttctccctgc tcagttcctc
    26161 caacgttgct gccatcacct ttgtctggtc gggcaaacca agttcatgca ataagtaact
    26221 gaacacccaa ggtcctgatg taacttgcaa aagagaactg accgctgatt ctaagagacg
    26281 ccgcaaggct aagggttctc cttcaaaaaa ataaccatcc tggcgattgt aagctaaagt
    26341 tacctgaaaa gcttgtaccc gatcacgcac caagtttaaa tcagcaataa cggaatttct
    26401 gctgagccgt aacaagcttt ccaagtggtt aatggagata aattctttag ccatcatgat
    26461 gtaaatgata atcatgtcca gacgctcatc ttgaaagaga tagtcgtcta aggtaggtag
    26521 catgcgattc aacaagtcct tttcagtaag gtctggcgga taaaccagct gcctgtccag
    26581 agcaataagg ggtaactgat agagtgacaa cgtgtcattg attttagcaa tatctgccag
    26641 tatatgacgt ttgcttgtct ttaatatgga ttcaaatgcc tccagtgaat gtcctttata
    26701 tttagaaaag agttgatagt ttctgatgag ctcatgagat agcatgtcat ctcctcctat
    26761 aatgtcagta taaaatgaag cgttttcatt ttcaagacaa attgagtcca attgtccgta
    26821 gtcatttttg actgcaattg gactcaacag gtgatatcat tgtaacaagt tcctcaaaag
    26881 gtggccaaaa ataggcaaat ctgggaattg attccctttt tcttcatgat acaacaaaag
    26941 atacctcatc actgagatac cttttgttgt taaataaaat catcaactgc ttaaaaaaac
    27001 agtcttagac cattctagcc aaatgtgtaa gttaacaaac aacatgtcaa aaatgaaaaa
    27061 gcccactgca taagcagagg actttttaaa cttgaaatta acggcgaagt ccaagagatt
    27121 ggatcaactc acggtaacgg ttaacatctg tacgacgtag gtatgccaac aagttacgac
    27181 ggtgaccaat ttttttcatc aatccacggt aagtagcgtg gtcttttttg tgttctttga
    27241 tgtggctgtt caagtggttg atttcccatg taagaactgc tacttgaact tcaactgaac
    27301 cagtatcgcc ttcgtgacgt gcatattgag caatgatttc attttttttc tcttttgaga
    27361 ttgccataat atattctcct ttttttgctt aatccgagtc ttaggattgg cactcctaaa
    27421 accaagaaaa agttggtttg tctttcgacc actctattct agcagattca taatattagg
    27481 tcaaccgtta tcctattatt ctaagctctc acatccaaac caccttaagc aacagaacta
    27541 gcaaggccaa atagccaaga ggctgttttt aaatcagaat aaacctgtgc gattgcttgc
    27601 ctaataagat aaccagctaa aagaggtaag atggctgatc cccgcacttc agctgataag
    27661 cggtaaccta gcatagtcat tcatttcttt ccaacaaagt gttcactgat aatattaatt
    27721 gctctagtat taacaaaccc aatccctaat ccatgaacac agcttattaa ccgatgcgac
    27781 ctgcctttat gacataggtc aagagttgac acaaaatgtt tgccaacaaa acattaccta
    27841 taccgtcaaa gagcgctttg cttcctatac ccttgaagcg caagccaacc aagaggttca
    27901 tctaaacctt acattattag caaaccgttt tggaacaagc gatcgccatc ttaagcacgt
    27961 acttaagcag cccatttttc agaggattat cgaacgcatc tgcttcaaaa cctgtcgtat
    28021 tgccgattta gacgctctaa ctcaattaag tgttaataac tagacctctg aggtatgcag
    28081 cccattaact tataatagca atcactctga ccactacctg acttttcttt aaaaaagaaa
    28141 tgatttgtga tatagtaaat ccagttaaaa aaaagtaaag gagtagacat gtctgcacaa
    28201 gataaattaa ttaaacctag tcatctgatt accatggatg acattattcg tgaagggaat
    28261 ccaactttaa gagctgtcgc caaagaagtg agcttaccct tgtgtgatga ggatatccta
    28321 ttaggagaaa agatgatgca atttttgaag cattctcaag atcctgtaat ggcggaaaaa
    28381 cttggactta gggccggtgt tggtctagca gctcctcaga ttgatgtgtc aaaacgtatc
    28441 atcgctgtgc tcgttcctaa tcttcctgat aaagaaggaa atcctcctaa agaagcctat
    28501 agctggcaag aagtcctgta taatcctaaa atcgtttctc attctgttca agatgcagcc
    28561 ctttctgatg gcgaaggttg tttatctgtc gatcgtgttg ttgaagggta tgtggttcgc
    28621 catgctcgtg ttacagtaga ctactatgat aaagaaggac aacagcatcg catcaaatta
    28681 aagggataca acgctattgt ggttcaacat gaaattgacc atatcaatgg tgttttgttc
    28741 tacgatcgga tcaatgctaa aaaccccttt gaaaccaaag aagagctctt gattttagac
    28801 taacaaaaaa ggccctttag cctttttttg ttagtctaaa tagccgctaa aagagcctct
    28861 gcctgagata gcaagtttgc ttttgtctct tttgaaatct caagccttcc tgttccccag
    28921 gcatcaggat tcacagttgc ttttgtaaac tctcctgcta ctgaagttcg gataaacggc
    28981 aatagtgctt taaactgatc aaatacttga tcatgcccgc catttgcaac tgaagagacc
    29041 gtaaccacct taccgccaat agcagatggg cccgtcggat cagacaaatc aagagcacga
    29101 gacaaccagt ctagcaggtt tttaacagaa cctggaatag agaagttgta aactggtgta
    29161 aaaatccaga tagcatccgc tgactgaaca gcttgacgag cgtcaacaac tggtaaaggt
    29221 acattagctt cgatatcttg attcaaaaca ggaacgtctt tccaatttaa gtaagatact
    29281 actgcttgat gttccagagc tttttgtgct tgagccgcta attgatggtt aaaagaccct
    29341 tcacgaagcg agccaacaat aaataaaata tgtttcatct ctttgtcctc tttttttaaa
    29401 gatcgtttta aagctttcta aaagtggtat ttcattgata cttctttttg aaatttatca
    29461 ctatgtagta attatatctt ctctaagaag agctgtaaag agatttgttt ataattgttt
    29521 gaactttttg agaagttcaa tcagacaaag ttgttctttt tcagtcaaaa cagcaaaggc
    29581 ttcttccacc cgtgcgacgt gtttaggcaa aacagcttca atcaagcgag tacccttatc
    29641 tgttaaggta accacatagg ctcttttatc cgttttgtct ttgcacttgc taatccagcc
    29701 attacgttcc atattattta gtacaacagt catattcccc gaagtcgcta acaaagaatc
    29761 aatcaagtga ttgattcgca tacagccttt agtatatagt acttcaagca cactgaactg
    29821 cgtggctgtt aaatctgcct ttttaaaaat atcagcccca aaagcatcta gcgttcgctg
    29881 agctttacga aacaccacca tggctttcaa agcggtgttc ttatctaaat gactcatttg
    29941 atttcctcgc tttatacggt atgtcacaat ttagtatcag agagattaga aagtgatctt
    30001 gtccctcttc ttttatgact ttgcatatga ctaccatcat gataatctag aaaaagtcat
    30061 caaaaagact taattgatta tcttctggca tatttcctaa aatacccatc tcatccattt
    30121 tctcaacgag cgttgacgat gccccgcctc gtttacgcaa ttccatttta gagaggaatt
    30181 cgccttcttg acgagcttta acgatttgct tggccacgtt ttcacccaga ccttctaacg
    30241 ctataaatgg agggataagg gtatctcctt tgatttggaa ttctatagca tcacttttgt
    30301 aaaggtctaa tttgccaaac ttaaagccgc gttctaacat ttcgttgaca atctccaagg
    30361 ttgtaaagag gtcattttcc acattggtgg cttcattatt tttacgtttt atagtaatat
    30421 cttccattct tgctttaaca gcatctaagc caccactcat ggtttttaat tcaaaagcct
    30481 tcgcacgaat agagaaataa gcacaataat acataatggg gtggtgcacc ttgaaataag
    30541 ccacccgaag ggccatcaag acataggccg ccgcatgggc tttagggaac atgtacttga
    30601 tttttccaca cgattcaatg taccagtcgg gcacattgtt ttctcgcatg gcatcaatat
    30661 agccattacg ttcttcctca gaaattttca gccagaggcc cttacgcaca cgctccataa
    30721 tggtaaaggc cattttcggt tctaagcctg cgtgcatgag gtaaaccatg atgtcgtcac
    30781 gacaaccgat aacggttttt agggtcgcaa tgccttcttt aatcaaatct tgtgcattac
    30841 caagccaaac atcggttcca tgagatagtc cagacaactg caaaagctcc gcaaaagtgg
    30901 tcggatgcgt ctcattaacc atgccgcgaa caaagttggt tccaaattct ggaatgccta
    30961 gcataccagt cggtgtccca atttgttccg gggtaacgcc caaaatctct gtcccagaaa
    31021 agagagccat aacacctgga tcatcagcag ggatagtaat aggatcaatg cccgataaat
    31081 cctgaagttt acgaatcatg gtcggatcat catgccctag gatatcaagt ttcaagacgt
    31141 tttcatcaat atcatggaag ttaaagtgag ttgtctgcca agaagccgtt acatcatctg
    31201 ctggatattg cacgggggta aaatcataaa catccatgta attaggaata acaacaatcc
    31261 ccccagggtg ctgcccagtc gttcgtttca caccagcagc acctgctgct agacgatcca
    31321 cctcagcatc acgatagaac ttgccatagt cgcgttcata gcctttgaca aatccataag
    31381 ctgttttttc tgctacggta ccaactgttc cagcacgaaa ggcgtattcg tcaccaaaaa
    31441 tatctcggac atccaaatgg gcactgggct ggtcatcacc agagaagttc aaatcaatat
    31501 cgggcacctt atccccatca aacccaagaa aggtctcaaa gggaatgtct tgcccatctt
    31561 tttgataagg ggtgccacat ttcggacagg gtttattagg caaatcatag ccagatccaa
    31621 ctgacccatc tgtgataaat tcagaatgtt ggcaggacgg gcaaacgtag tgaggcggca
    31681 taggattaac ctcagtaatc ccaatcatgg tggcgacaaa gctagaccct acagatcccc
    31741 tagagccaac cagatagcct cgctcatttg accggttaac aagcatttgg gaagcgagat
    31801 aaatcacagc aaaaccgttc cccaagatag aggttaactc tttttcaatg cgtaaatcaa
    31861 taatatctgg gagaggatta ccataaattt caaaggcttt ttgataggtt aattcggcaa
    31921 ccgtctcttc ggccttgtca atatacgggg tgtaaagatc gcccttaacc acttccactt
    31981 cctcaatacg gtccgcaaaa tcctgagtat tttctacaac cacttgataa gcgaggtctt
    32041 ttccaagaaa ggcaaactca tccagcattt cattggttgt tctaaagtgc gctttaggta
    32101 ggggagcagg ttgggcccct tccccacggc cgattgttct attaatcatg gcaccctgac
    32161 cgagactacg cacaataatt tcacggtaaa tctcttcttc aggctctagg taatgcacat
    32221 ttccagtggc aagtacaggt ttattagctc gtttccctac ttcaatgagg tcacgaatca
    32281 cctgctcaat acctgcttgg tctttgatta attcacggac aaccaatggc tggtaaatgg
    32341 ctggtggcat gatttcgata aaatcataat acttagccaa atcaaccgcc gcatcaattc
    32401 ctttagtcag aacggcatca aaaacctcgc cgtcagaaca agctgtacca agtaacaaac
    32461 cctctctgtg agcatctaag acggttcttg gaatacgcgg caccccttca aaatatttaa
    32521 tattggaaag gctgaccaac ttaaacatat ttttaaggcc aacctgattt tgcacataga
    32581 tagtcgcatg cttaatccgc gcttttttgt aagaatcctc agctaccaaa tctgtattga
    32641 gttgcaaaag atttttgatg ccatgctttt ctctggcatc tcttagaaaa ataaacaaaa
    32701 gacgtcctgt ggcttccgcg tcgtaattgg ccatatgatg gtggtctaga ctcacttgga
    32761 aacgcttggt gagcggtccc aaaccgtgac gcttgtactc aggatacaag tttctagcaa
    32821 attctaaggt atcaatcaca ggctgtgtga ttttgggcaa gtcgtggcgt tcataattgg
    32881 cgttcataaa gcccacgtca aaactggcgt tgtgggcaac caagatacta tctttgcaaa
    32941 agtcctgaaa agcttttagg acggtaacca atggcttggc gtcctgcaaa tgcttatcgg
    33001 taatccctgt caattcggtg gtaaaggcag aaagaggatg cccaggatca atgaattcat
    33061 caaactgctc tacaatattg cctttaaaca ttttggaagc cgcaatctga atcaggtcat
    33121 tattcatagc agatagacct gtggtttcca cgtcaaagac cacataggta gcttcgtgca
    33181 aatccatatc aacaggatca taagaaatag gcaccttgtc ctcaacaata ttggcttcta
    33241 ggccaaaaat agccttaatt ccagctttgc gagccctatg gtagccatga ggaaaacttt
    33301 gcacattagc atggtcggta atagcaaccg ccttgtgtcc ccacttggct gccgtatcaa
    33361 tcaagctttc tactgtcggt aaggcgtcca tggtagacat attggtgtgg gcatgaagtt
    33421 cgacccgctt ttgcccttct ggcatcaggt ctttgcggtc atgatggaca atttctttga
    33481 cctgctgcac attcatggtg agactcttcg taaaaggatt ggtctcaata ttcccttgta
    33541 cccgtaacca agctccctta gcaatcatgt caaatttacg gagctcctca tcgtctttgg
    33601 cccatttttg cagagcaaac gaggaggtat agtctgtcat tttaaagttg atgatatggc
    33661 gacctgttct agtcgtttta cgctccacat caaaaaccat accctcaaag acaatccggt
    33721 tttcttcggt ctcaatctca atcattggtg tgatggtcgc tttttcaaac cctgcctgac
    33781 gcttagctgc tcgttccttg tagtcaaact taggagcagg tgtggcttcc tcaactggtg
    33841 gcatcatggc ttcaagggat ttttgggctt ccaaattatc ctgcacagcc tttttcacaa
    33901 gagcctgacg actggaaaca aaatccttgg tcaaatgctc agtcatctcc tgatctgaca
    33961 ccatatctat ggtcaaggtg ccaaagccaa aggcttcaaa ttgcttgacc agattaggca
    34021 gatgactgtt tctaaaatga tcgttattca caaaacctgg cgctgcaata atgagtttgt
    34081 catcctcata agtcactttg agctttgaga aagaagattt aaaactagca ctattacacg
    34141 gtgcatgctc aaaagcttct tggtaataag cttgaagcag atcatctgaa taatccacct
    34201 gagcagcttg gatatcaaag gttaccttaa tgtcagccgc ctcaaaagtt cttatcaaac
    34261 gatcatgtaa ttcacgataa gttgcaatcg gtaaaaccgc cgcaaaggca aaatgaaatt
    34321 cccacaagcg tgacaccgaa tgcaccttta cctcgataat atcagcagat gaaaaggcac
    34381 ttgaacgtct catgtcaagt ggcatttcta tctggtccat caatttagcg aataaatctg
    34441 acataatttt ctcctgattt tactaggctt tattttatca caattcaaga tgattttcga
    34501 gttttagatg gaaagaaaag cagggctttc ttctgacgtt ctttggctaa tttattccaa
    34561 gtgatagctc agctgaggtc atttttttgc caatcttttg tcgccaagcg cttttggtaa
    34621 agcaaaagcc actctgtatc agacaagttc acagcaaaaa gaggccactc taaacaggca
    34681 gcctcttcat ttctttttta gtgttctttt gttaaaattt caagggtttc gatgaggttt
    34741 tctgcattaa cttcaatgct gtcacctgtt gccttgattt taatttccac gataccttca
    34801 gcggcttttt taccaacagt gacacgaatt ggcaaaccaa tcaaatcgct atcagagaat
    34861 tttgaaccga cgcgttcatt acggtcatct gtcaagacat catacccttt agccatcaag
    34921 tcagcttcta acttcgccgt caagtcttgg gccacttgat ctttgacatt aactgtaatc
    34981 aaatgcacgt caaatggggc taattcttta gggaagttaa taccccaagc ataacggtaa
    35041 tcgccttttg gtgtcttgtt cacaaagaga cgggcatgct gttcaatgac agcagacaaa
    35101 atgcggctaa ccccgatacc ataacaaccc atcacaattg gaactgttct gccattttca
    35161 tcaagaatcg ttgctcccat gctgtctgaa taacgagtgc ccaacttgaa gatatgaccg
    35221 acttcgatac cacgtgcaaa ctggagaaca ccatgaccat ccggagacat ttccccttct
    35281 ttgacttcac gaatatctac atattcagcc tggaagtcac gtcctggatt gactcctgtc
    35341 acatggaaac catccttgtt agctcctgca acggcattgg taaggttttg cactttgcgg
    35401 tcagccacaa tgcgactacc ttgtgccaag ttgacaggcc caagtgagcc aaaacctgca
    35461 ccaaagaaag cacgggcttc ttcttcacta gctggctcta aaaaatcagc agctagatag
    35521 ttttttaatt taacggtatt gatatggtca tttccaacta gcaaagcaac aacaggttca
    35581 ttatctgcca caaaaagcaa ggttttgatg gtttgtgttt catctactga aagaaaagca
    35641 gccacttcat caatcgtttt gcaatgtggt gtctcaactt ctgccaaggc atcttcagca
    35701 gctacttttg aggacggttt gtattcgtta gttgccatct caaggttggc agcgtagctt
    35761 gattctgttg aataagcaat agtatcttca cctgaaatca tccaagcagc caattctgcc
    35821 ttaatatctt ctaagacctc ttttggtata tcatccattg acgcaataga cttgtcaaga
    35881 accacccagc gatcaaggtc cgtacgagct ggtgtaacgg ccataaattc ttgggaatct
    35941 ttccccccca tggcaccgcc atctccaata atccctttga aatctagacc agctctggta
    36001 aaaatagctt cgtaagcttg acgataatct tcgtaggtca catctaaatc ttcatagttg
    36061 tgatggaaac tatagccgtc tttcatgata aactcacggg tacgaagcaa gccattacgc
    36121 ggacgttttt catcacgata cttagactga atttgataaa ggtttaaggg caattgcttg
    36181 taagatttga ccgcatcacg caccaaagtc gtaaaggttt cttcgtgggt cggacccaag
    36241 ataaagtctg agttatcacg gtttttaagc ttatagaggt cctctccata ggtctcataa
    36301 cggcctgatt cacgccagag atcagctgtc aaaagagctg gtgccaacat ttcaacagca
    36361 ccgatctttt caaactcttc acgcatgatg gtcttgaatt tctcaatggt acgatttgcc
    36421 agtggtaaat aagcatagat accagcagaa acttggcgca cataaccggc acgcaccata
    36481 agcgcgtggc tgataacctg ggcatcactt ggcatttcgc gcaaggttgg gataagcagt
    36541 ttactttgtt tcatagatta ttccgtttta atacatcccc ttatcctcaa acccttctgg
    36601 aaacatcaag taagagtgaa gcgggattat tcaataattt gaatgtgtat tcctttcatt
    36661 ttgagtaaga cctcaaccct tgcaaatctt agggttgagg tctttttcga tctttaacat
    36721 gataaggtca gcttaagatg acctatcttg attagaaaaa gacgcgcatg atatcattcc
    36781 atgtcacagc aatcatcaat acgaccatga tagcaacccc agctagggtg atataggcct
    36841 ctgtttcttg cttgataggc ttgcgacgaa tggcttcaat gatattcatc aagatttttc
    36901 ccccatcaag tgcaggaatc ggaatcaggt taaagatccc taaattgatc gaaagcattg
    36961 ccataagaga taagactgac tctaagccgt tttgagcagc ctgattggac atgtcataca
    37021 tggcaacagg tccacctaat ttattgagac taaagccagt gatcaatcct ttcaaagcat
    37081 tcaaaatagt aaacgcccca ctccaagcta attctagacc gcctaagagc ttatccttaa
    37141 atcctgtttt cagactcgcc ttaactccta tcgtgtatgt ctttgcatgc ttttgtggtt
    37201 tcacagccac cgtttttaag cgctgatgtg acttgtaggt gaccttaatg gtctgtgacg
    37261 ggcctagatc ccgtgttgca aggtctacag cctcagtgag atcattccag ctagtcacct
    37321 tataaccatt aattgcgaca atttggtcat tatctcgaag accagcctta gctgctgctc
    37381 cattttcttg aacacggaca tgattgctac taaaatctga catcccacct tgtaaaaaga
    37441 ccaagaggat aaaaaccaca atccctaaaa taaaattatt catgggacct gcaaaattgg
    37501 tgattaaacg tccgccaata ctagcatttt gatattgaac atcaagcgga gcaattctta
    37561 tctcagtacc gtcttcttca acaatagtag catcgtgagc gaccttatat gtcttggttt
    37621 catctaaaac caagccagta atactcagct gatcttctaa gtcatagcct gtaacatgca
    37681 ttgggagact cgttgggtct aacttacttt gagacaagtt gatgcgctta acaaaacctt
    37741 gctcattaag ggttaaactt gctggagtac ctgttttgat ctcagttttg tcatcacccc
    37801 aaccagccat tcgcacataa ccacccaagg gtaacatcct caaggtataa agagttcctc
    37861 cttggtcaac atgagaaaaa attttagggc ccattccaat ggcaaattct cttactagaa
    37921 tgcctgattt tttagcaaaa tagaaatgtc caaattcatg gacaatcact aaaataccaa
    37981 aaataataat aaaggttatt attcctaaca tagatttcct ttcagtctat tgcttttttg
    38041 agttttgtcg cgttgttgcg actccaaaca ttacctctta agattaaaat aaaccaaaga
    38101 gatgcatgat gggaaaaaca aaaatcatgg aatcaaagcg gtctaaaata ccaccatgtc
    38161 ccgggataag cttacctgaa tctttgacac caaagtgacg tttgagagca ctttcaacca
    38221 aatcaccaaa ttgggcaaaa atggaaaaaa gagcgaccaa aaccaacatg gtcaaaaagt
    38281 gatgtggcgc ataaacagac ctatcaatca ccataaagat aaaagacaca aggactgcac
    38341 aagcgatccc tcctaaacta ccttcaatgg ttttgtttgg agaaactgta ggcaatagtt
    38401 tgcgcctacc aaactgacgc ccaatcagat atgctccaat atcagtggcc caaacgataa
    38461 ataaggccaa aaagaccttg tcaattcctg acaggcgagc attgatcaaa tgttgaaaac
    38521 caatcccaac ataaaaacta gttgcaatcg gaaaggccgc atcatcaaat gaataagctc
    38581 tgctatttaa aacagtacct gccaaaatga aaaaaaccat taagctgtaa aaagcaacat
    38641 tcgcatcaat tggtaaaaaa gtgaggtaat ggtccatcgg aactgccaat acaaaggcgg
    38701 ctaacatggc aaaaacacct tcaaaagaaa agacttctaa tcttttcatt ttcagtaatt
    38761 ctgatacccc aatcatggcc aaaacgccta caaataattg gaatggtagg tttcctatta
    38821 tgagaaaagg taggaaaata gctactgcta ctccccccca aacaacacgt tctttcataa
    38881 ctatctcctt ttagaccttg ccaaaacgac gttggcggcg gttataatcc gcaatggcct
    38941 ttagtagctc tgccttttta aaatcaggcc acaagactgg tgtgaaataa aattcactgt
    39001 aagctgattg ccaaggcaag aaattgctca aacgcagctc accactggtg cgaataatca
    39061 aatcggggtc tcggtagaga tatggcagat ggtctgtcat caagtaatta gcaatcaaat
    39121 cctccgtaat atctcctgga tttaatttgg catctaagac atcttgagca ataaagcgaa
    39181 cagcactggt aatctcagca cgtcctccat aattgagagc aaaattcaaa attaaacccg
    39241 tattacgttt tgttttgtcg atggctgcat ttagcgctgc caaggtatct tcaggtaaac
    39301 gactagtctc tccaatcatt tgaatcttta cattgttctc atgaagcaca ggcacatatt
    39361 tgtcaaagaa ggttactggt aaattcatga taaaagacac ctcatcttgg ggtctggacc
    39421 agttttctgt cgaaaaggca tagactgtca gaactttaac ccccaactca gatgcagtaa
    39481 tcgttacttc ttgtaaagca tccatcccag ctttatgccc aaacactcta ggttttagtc
    39541 tttttttggc ccaacgtcca ttaccatcca tgataattcc aatatgcttt gggatactcc
    39601 ccaaaacttt ttttgttgat ttcgctttta atccaaacat gttttttcct tattgataag
    39661 catacttatc tcatatttta tcatatttca cgccttttgg cttgtttttt atcaattaga
    39721 tcttatcttc acaaaatgtt cacctatcct ctgatagatg tgtcagcttt tcatgaacct
    39781 gttagcatca gaataactgt ttcagtccag ggatcaaaac aaaaaagttt ggcatatttc
    39841 atccaaactt tccccgcctt aatggctttc aatggcactg tcagtgtcag tgctatctac
    39901 tgtctcaaca gcaggctcat taacaacact ggtttcttct gtttcaacca gtgtcgtttc
    39961 tgtggtagct tttgttacaa tacgcttaat agctgatagc tcaaatgtca aaaagacgcc
    40021 atccacatct aacacaatct ttttagctgt tgtatcgact tcgtcaacaa tcgcaaacat
    40081 gccaccaatc gttacgactt cgtctccttt ttcaatagcg tttaactggt tttggcgttc
    40141 ttgcgcttgt tttttttgtt gacgttgcat aaaccaaatc aatccaagca tcactacaaa
    40201 cattaaaatt gttggaaatc ccataatgtc tcctttttat tctataaaag tcatgtctta
    40261 ctatatcaaa aatcaagagg tatagcaaga ctagacgtta cctgatttct tctttaaacg
    40321 agacgctaag cgcagtcctt tactcacgag tcaaaaaagc taagatgtca tctggtgtca
    40381 aacttgaatc acatacagcc ctttggtgct gatcgtaact aaccagcaaa gccggaactg
    40441 tcactaattg gtagttctca cgaaaggcag caagctcagc agcatcagca gcgttttcac
    40501 tatccacaaa atagacttct ttttgattat ctgttgccac ttgtgccaac ttaggagcaa
    40561 agcggcgaca ataaggacag ctcgaacgtc ctaaaaagac aatcatgtct ttgccactag
    40621 caatagctga tgtgacttct gcaacactcg acggtataaa attcgcaacg atttcttcaa
    40681 atgtcatgga tgtctccttc tcttttggtc tcatgataca ctaaagtagc aattttctca
    40741 atcacaaggt cttttcacag ctcagagctt ttatagccgt caaaaagaat ccgcaaacag
    40801 caatcttttt gtctctatta acaccaccct aatctttttg gcgcttcatc aacaggctaa
    40861 tagccaataa aagcagagct actccagctg ctaataagcc ttttgaggag gcttcacccg
    40921 tttttgggag cttttggttt gttttagctc tttttggcct agtcattaat gatttatcta
    40981 ggtcttttga gccatctggt gttttggttt gatgattgtc tgtctgactc ttttgctgag
    41041 agggattagc tgtttttgag gatactttga ggaccagggc agttaggcca tcaatagtca
    41101 agccttcttt ggtaaactgg actcccttag gcttagcaat ggcagtaaca ccagcctgct
    41161 cagcatcagc aagcacttgg gctcctagca aatagcggta ggcttgaggt aaaactacct
    41221 tgcgggtctt gttgtctgca ttgacaaaga cagcatagcg atctccattt gatgccaccg
    41281 tttggtaacc catgataagg tcctcttgtt gaataccatc ttgtcctgct tgggtgatca
    41341 aggtcacatc ccgatctacc tcagctttgg ttgcttttgt aaaggcatct gttgagcgac
    41401 gcaacgcaat taggccctgt gtataggctt ttgtttggtt gctaatcggg tgagctatgg
    41461 aatctgttgc ctttgcccag tcaaaatgat tgaccgcatc agacgaatca taagaatcgt
    41521 ggatgaagta agggtatttc gctacagcat caatcagagt cgccttattt ggcaccttgt
    41581 catcagacac ctttgtcctg tagtcgggat ttagaagctg cttggttcgt ccatattcct
    41641 gaccagaatg gataaaggca gtcccttgag cagttaaaat catggtattt cctagacgaa
    41701 tacgcttatg aatctcttct tcagccactt taggatcctt attgatggat ttggcaatga
    41761 catcatgtaa ggtcaggttg tcatgggctg caatatactg cactacatct cctggggcat
    41821 ctgcttcaaa gttactaggc tgtgctttga tcgttttgaa cagtccttct agattttttg
    41881 cgccaccagt aataaaggct gctgtgcctt catttgggaa acctgacttg agggtatttc
    41941 tgatatcatc agagaaaaca ccgaccgtat tggttgcttt catccaatct tgatctgccg
    42001 caatttcttt tttcccctca tcaccttggt aggtacgcca gccttcgcca atcataatgg
    42061 tatttggatt aatggctttg gctgccttaa aggcttgctc aatagctgcc gcatcatggt
    42121 cacccatcat gtcgaaacga aaaccatcta ccttgaattc acgagtcaga taagcaatcg
    42181 aatccaccaa gatacgacga ctcatggcat gtgtcgttcc tagacgacct ccgccaaaac
    42241 tctctctagc tgtgccatca gcattcataa aatgatagta gttgggttcc aaatcttcaa
    42301 agagataggt tctagccgtg tggttataaa ccacatcaaa aataacaccc ataccacgtt
    42361 tgtgaatctc attgacaagg tttttaagct ctgcgatacg taaagcaggg tcgttaggat
    42421 ttaccgaata catgccagaa agggcaaagt agtgttgtgg gtcataaccc cagttgtaat
    42481 tattgtctga agacgtgtag gctgttgagc ggctcttgtc cagctcattg gcataaaaat
    42541 aactcaaaac cggtagcaat tgaacgtggg taacccccaa gtctttgaga tagtctagct
    42601 gttcaacgaa agctgaaaaa gtcccaaaag ggtgtgttaa cttgccttct agagccttat
    42661 ctgacgtaaa atctcgcaca tgtgcttcat agataatagc gtcttcacgc tttttaaagt
    42721 tgttaatttt ggcaaaatca aggcctgttg gtcctagttt gcttggatca ataaaggcag
    42781 cttttgctgt tttgatgtca tcagtagcag tcgcatcatt ccaggcagcg agagatttgg
    42841 cgtaaggatc caaaaccatg actttttcct gaccgcgcgt gatttcataa aggtaatagt
    42901 agcctgtgta atcactaatg cccttgatac tatcagaagt cagatgagct ctccaaacac
    42961 ccttgtccga cttggtcaaa tcagcttgac caaccaccct tgtctgatct tgtttatcat
    43021 aaacgacaac cttaacagta tcagcacttg gagaccatag cgctaaatca acagaaccat
    43081 ccttagctag ggtagctcca agttcaccat cgtaagcata gagtttatct tttaattgcc
    43141 aggattggcg agcgacttgg ctaacttctc caaatgtaac cgtgtagact gcaccttgcg
    43201 ccttaaaatc accctttaat gtcattacag acttatccct gtcaagtgtc aactcatcaa
    43261 ttgcaacagt tttccctgct ttgtcagtga tcttgatgtt ttgtttcacc gcatcttcat
    43321 caagcccatc taaggtcgta aaaatggctt taatctcatt tggcgtggtt tgttcagctc
    43381 ctttgagact aacctgatca atataataag gattgttgta gacttttggg tcagtgtctt
    43441 tgacaaaaat ctgagtatga ttgtctaact ctttaaacag ataatctttt ggttgcactt
    43501 taatagcatc tcctgtctta cttttatcaa ggattaaaaa tccgatttcg ttagctcctt
    43561 cttttaaggg gacatcaaca taagctccat aaggcccttt atgtgacaag tcaagtccat
    43621 ttggccagtc ggtcgttggg gttttgacat ctttaaaggt ccagacagct aagttatcgt
    43681 agtgtcccga ttgattgtgg tagttgattc gaaggtaacc ttttttcaaa ggtcgataag
    43741 cgtgcgcatg gtaattctcg tctatccaaa cttcattcat ttttggtgtg agaagcgaga
    43801 tgtgctggtc ctttgaaaga ttctctccag ctttattatt aatgagataa gacacttgct
    43861 ggcggtgttt agctgctagt ggcacatcta gataatagcc atagtcatct tttttcgctt
    43921 ttgtcatggt gatagcacca tttggccaat cctttgaagg ttgatccaca tctccccaca
    43981 cccaaagtcc caagcttcct agcgactcac cagctggcaa tgttttaaaa tgcatacgca
    44041 aatgatgctc agcaatacta gcaggattag ctaacgagct gtctaaaact ggttttgcct
    44101 ccttgctaga cgattgaaga ccatcaccag atttagcatt gccatctcct tgttgagctt
    44161 catcgcgggc tagtccaaca atggttttag cagcaacttg atgtgttaag atactagggg
    44221 tgcaccctaa cacgacagtc ccagttgcag cgattacaaa acctatcccc cactttttta
    44281 acagatattg atagcgcttt gatccttggt tgactttctt tttcataaga cctccattta
    44341 gtataacatt caaacaagac tatcttatca cattgcgcaa gcgcttgcaa gacatgccgc
    44401 acttcaactt agaacacagc tcaaacaatg actatcaccc cttaatagga tctcaaaaat
    44461 caaacagcca aaacgagttt ccactcgctt ggctgttggg taaactcaaa tcacatacaa
    44521 ctgctaggcc ttcagtttga gacaaaaggc atcccaaggt tgtaagtgct tggtctcgaa
    44581 caccgtgtcc acatctgtat tggtgatgat cacttgtgca cctgttaagt ctttggcaaa
    44641 gacttgctcc tcatcagaca cattgacaac aatcacatac gtctcttctc caagctgacg
    44701 ctgataagca aacactttat cagctgttgg caagaggtga taatccgctt caactagcca
    44761 gtcctgctcc ttacgcaagg caatcaattt ttggtaggtg taaaaaatag agtctggatc
    44821 agcaagggca tcggccacat tgatgtcttg gtagttgggg ttgaccggca accaagtctc
    44881 ttttgcttca gaaaagccag catgtgtgtc cttagaccac tgcatcggcg ttctagcatt
    44941 gtctcgtcct accttacgga tgctatccat cacacgcgca gctgacactc catttcccat
    45001 agcttcctta gcatagttaa gggactcaat atcattaacc tcggttaagt ctttaaaagg
    45061 ataattggtc atgccaatct cttctccttg gtaaatgtaa ggggttcctc gcatcaaatg
    45121 cagtaaaata gctaaagcct tggctgattt ttctcggtaa gtgctgtcat tgccccaaat
    45181 ggataagacc cgtggcaagt catggttatt ccaaaaaagc gaattccagc cttctcccaa
    45241 ctttaattct gtctgccact tgctgaaaat agttttaaga gcaggaacat ccagttcttc
    45301 cgcataatcc cacttgggag cgtcaggttt atgttgaaga cccacatgct caaattggaa
    45361 gaccatggat aactctttgt tttcagggcg agaatactgg cgagcaattt caggcgttgc
    45421 tccccaagtc tcaccgacag tcatcacgtc atgattgcca aaggttgctt gattcatttc
    45481 tttgaggtaa tcgtgtaagc gcggcccgtt acccgtgatt tcagaatctg gaaccttccc
    45541 aatcaggtca atcacgtcca tacgaaaacc accgatgcct ttagcaatcc agaaattcat
    45601 catatcgtaa attttatgac gtaaagcttc attctcccag ttgagatctg gctgacgttt
    45661 gctaaaaagg tgcaaataat attggcctga tgcattatca tattcccaag ctgagccact
    45721 aaaaatagaa gtcaaatcgt taggttcatc tcgccaaata taaaaatcac gctcaggact
    45781 attagggttt tccctagctt caacaaacca agcatgctca tcagacgtat ggttgaccac
    45841 caagtccatg ataatcttaa tgcctcgctc attagctgct gctaagagct catccatatc
    45901 tgccatgtct ccaaaaacat cagcaatcgc ttcatagtca gagatgtcat aaccattgtc
    45961 atccattgga ctttggtaaa caggagataa ccaaatggct gtaatgccaa gtttttgcaa
    46021 gtagtccagt tgactggtaa tgccttttaa atcaccaata ccattgccgc tggtgtcttt
    46081 aaaggatctc gggtaaatct gataaatcgt agctttatgc caccagtgtt tcttcattat
    46141 tcttctccta ttaatcattc attcatcttt tattttaacg ttttttctct ttttgtgcaa
    46201 tcgtttgcgc aaatgacatg ataaaaaggt tggaacccaa cctttttatg aatatcgctt
    46261 acaccttaac gaatagcttg ttcggtatct ctgtcaaaga aatgcccttt agccacatta
    46321 aaggtcaagc taacttgctc ccctggacta tggaaatcac gcgcatctac acgagaagca
    46381 aactcagttt gtccaagctt gacatagagc atcgtctcag agcccagtaa ctcagaaacc
    46441 aaaacttctg ctgtgacagt tgcacttgga taagtatcat gcacgatttg tctactagaa
    46501 atgtcttctg gacgaatacc aaaagtgact tttttcccct tgtaaccagc cgcttcaagc
    46561 atttttgctt gaccttcagg aatcgcaata gcaagaccat cttcactcac aatgcggcca
    46621 tctttaactt ccacttcaaa gaagttcatg gcagggcttc cgataaaacc ggcaacgaat
    46681 ttattagctg gaaggttata caattcttgt ggtgaaccca cttgctcaat tttaccaatc
    46741 gttccattac cttgagggtt tttagtggca ctcatgataa cgatacgatc tgccaaagtc
    46801 atggcttctg tctggtcatg ggtaacgtaa atagttgttg agccgatacg acggtgaatt
    46861 ttagcaattt cagcacgcat ggagacacga agcttcgcat ccaagtttga caaaggctcg
    46921 tccatcaaga acactttagc atcacggaca atagcacgtc ccatagccac acgctgacgt
    46981 tgaccaccag acaggtcagc aggtttacgc tctaaaaatt cggtcaaacc aagaatctgc
    47041 gcagcttcct tgacacgacg gtcaatatca tcttttttgt acttacgtaa tttcaaacca
    47101 aaggccatgt tatcatacac ggtcatgtgt gggtaaaggg cgtagttttg aaagaccatg
    47161 gcaatatcac ggtctttagg agacttgtca ttcaccactt cgccaccgat ttttaactct
    47221 ccttcagaaa tgtcctcaag acctgcaatc atacgaagag tggttgattt accacatcct
    47281 gaaggtccaa caaagacgat aaattctttg tctttaatgt ccaagtcaaa atcttcaacg
    47341 gcataatgag tagtatttgg gtatttttta tagatgtgat ttagatttaa ttcaaccatg
    47401 tataactcct atttagccag caaatgttct agctgactgc aaagctagaa accgctgata
    47461 tcttttatat gatgctttta ttataacaaa aatagcgctt tcaaaacaga gacaaagcga
    47521 ccaaacttct ttgggcaact tgcacaatag cagggctagt caagtcgtaa gggaccataa
    47581 accaagaaaa ctatgtaaac acaggccggt cacaggagcc aaaacaagct gaacgatcta
    47641 tcattctcct tatcagctac agtccaggca gcaaaaaaga aagctctgtg gctttcctaa
    47701 tatttcaaca aaaagagatg agcaaaggct aaatcatcca attgcttcaa gtggagtcct
    47761 gattgctgag caaatttatc aagtttgtat tgcaaggagt tacggtgaat atacaagcgc
    47821 tgcgctgtct gcaccagatt gccgtgttct gaccatagcg cgtgaaccac atcggccata
    47881 tcactttgag gacttaggga ttggtgaaac tgacgcgtca atgccaaaaa agtgtggtgc
    47941 gacagcaaag accacagcat cagtcctgaa aatgttaata attttctgcc tgattgttgc
    48001 cttaaatagg ccgttaacag ctgacattcc tcctcaaaac actctctcaa tgtacccgca
    48061 gctacttgat accaggcatt tccaacaaac atggttaagg ccaaaccaaa atcactttct
    48121 agcgttggca ggagatcttc aagccagcgc aagaccttta ttgagattgc ttgtcggcat
    48181 aaaaatgccg tctgagttgc actgataggc aaaatgacct caagccctgc aatcatctgt
    48241 tggagcaact caactaagtc atctggtaaa gcctgctgat gattgaggta aatgaactga
    48301 gaagtctcaa acagctgagg gggattgccc tgatgatcaa gcaaataccg ctgccaaggt
    48361 gaccctagcg gctgcaagac ctcacactga cccagcccca cctcaagtaa taagcgctct
    48421 ttttcggata aacatgattt gggaaaatgc acatattggt ccccttcttt gacactcacc
    48481 cactctttat ctcctaacgg atgagggcct acttgcatct ctggaaaata gtcttttagc
    48541 tccatagcca ttcctttcat gatggtcttt aaaccattat aacacatgac tctttatcac
    48601 acagttcggt ttgttgtcag cacgattttg tattttctgt ctttttaatc attaaagcta
    48661 aataagtgtt attcattttt agcaagaaca ttcaatcaaa tagctattta tcggaagatt
    48721 aatttatgtt tatactaaaa caggtattat ttctcttttt tcattatcat gacgttatca
    48781 ttaagatatc atttaaaaac aattattagg tttttgtttg tatctataaa attattatgt
    48841 tattctaata atggggattg aaacttaact tttaggaggt ttctatgaaa aattacttat
    48901 cttttgggat gtttgcactg ctgtttgcat taacatttgg aacagtcaag cctgtccaag
    48961 ctattgctgg acctgaatgg ctgctagacc gtccatctgt caacaacagc caattagttg
    49021 ttagcgttgc tggtactgtt gaggggacga atcaagaaat tagccttaaa ttttttgaaa
    49081 ttgacctaac atcacaacct gctcatggag gaaagacaga gcaaggctta agtccaaaat
    49141 caaaaccatt tgctacaaat agtagcgcaa tgccacataa acttgaaaaa gctgacttat
    49201 taaaggctat tcaagaacaa ttgatcgcta acgttcacag taacgacggc tactttgagg
    49261 tcattgattt tgcaagcgat gcaaccatta ctgatcgaaa cggcaaggtc tactttgctg
    49321 accgagatga ttcggtaacc ttgccgaccc aacctgtcca agaatttttg ctaagcgggc
    49381 atgtgcgcgt tagaccgtat caacctaaag ccgttcacaa ctcagctgaa cgcgttaacg
    49441 tcaactatga agtgagcttt gtctccgaaa caggaaattt agactttacg ccatcgttaa
    49501 aagagcaata ccatttgacc acactggcag ttggtgactc tctttcatca caagagttag
    49561 cagcaattgc ccaatttatc ctatcaaaaa aacatccaga ttatatcatt acaaaacgtg
    49621 actcctcaat cgtcactcat gacaatgaca ttttccgtac gattttacca atggatcaag
    49681 agtttactta ccatatcaaa gaccgggaac aagcttatgg gatcaataaa aaatctggtc
    49741 aggaagaaaa aatgaacaac actgacctta tctctgagaa atattacatc cttaaaaaag
    49801 gggaaaagcc gtatgatccc tttgatcgca gtcacttgaa actgttcacc atcaattacg
    49861 ttgatgtcaa caccaacaaa ttgctaaaaa gcgagcagct cttaacagct agcgaacata
    49921 acttagactt cagagattta tacgatcctc gtgataaggc taaactactc tacaacaatc
    49981 tcgatgcttt tggtattatg gactatacct taactggaaa agtagaggat aatcacaatg
    50041 acaccaaccg tatcataacc gtttatatgg gcaagcgacc cgaaggagag aatgctagct
    50101 atcatttagc ttatgataaa gatcgttata ccgaagaaga acgagaagtt tacagctacc
    50161 tgcgttatac agggacacct atacctgata accctaacga caaataacca cgatcttcta
    50221 aaacgatgag attaactgac aaaaaaagca agcaacacgc tatcaacagt tgcttgcttt
    50281 tttctaacct cttagttgta gagactagtg acatttcgtg tctaaaataa tcgtaactgg
    50341 tccatcattg atgagactaa cctgcatatc tgccccaaaa acgccacgct caactggcac
    50401 aaactctgcc aattgttcat taaagctatc atacaactgg ctagccaaat ctggcttagc
    50461 agcaccagta aaggcaggac ggtttccctt tttggtgtca gcataaaggg taaattgcga
    50521 cacagataag atactgccct tgatgtcttg gatagactga ttcatcttgc catcagcatc
    50581 tgaaaaaatg cgcatgttga ctatttttcg cacagcgtaa gccaaatctt ctgcattatc
    50641 atctggtccc acacctacta acaacaacaa cccttggtta atagcgccag caattttccc
    50701 atcaatagaa acagaggcct ctttgacccg ttgcaagaca agtttcatgg ttttatcctt
    50761 tcttagccat tggtccgctt cacgctataa acatctggaa ctgccttgat tttttcgaca
    50821 acagtggtca gatgcatcag atttggaatg ccaaagctca cgtgaatatt agcaaacttc
    50881 atgtccttgg tcggctgagc attgactgtc gatatgctct tggttgagtt tgataaaatt
    50941 tggagcacat cattgagcag gccactacga ttgagcccat agatatcaat ttcagcctga
    51001 taatctttac tcgaattgtc caaatcccac tcgacctcaa tcaagcgttc ttggtagcca
    51061 tcttgactct taatgttatg acagtccgat ctgtgaatcg caatgccacg ccctttggta
    51121 atatagccgt caataggatc accaggtaca ggattacaac acttggcaat ccgcatcaag
    51181 aggcctgatg ctccttggat aatgacccca ttttcactgc gaaccttgag cacatctttg
    51241 ttttcgtgtt tgacctcacc gcccttaacc aattcttcag cttctgcttt agccttggcc
    51301 ctttcttctt cacggcgctc tttttcggtt aacttgttaa agacactgat aggactaatg
    51361 tcaccaaacc caacggctgc atagagtgat tcttcgctct tcacactgac ttttggaagg
    51421 atcgcttcaa tgcgtttttt gtcaaggtat ttattggcaa catagccctg ctcttgaaaa
    51481 taagacacca acaaatcacg gcctttattc actgacaatt ccttgtcttg atttttaaag
    51541 aactgacgaa ttttgttgcg agccttattg gttttgacca gttttaccca gtctcgacta
    51601 gggccaaagg aattggcatt ggtgatgatt tcaaccacat ctcctgtttt taacttggca
    51661 gtgagaggaa ccatacgtcc attgactttg gcacctgttg ctttttcacc gatttgcgta
    51721 tggatcgcat aagcaaaatc aataggacct gattcttttg gcaactcctg aacggcccct
    51781 gtcggtgtaa agacataaat ccgttcagaa aaaatgtctt ctttgaccga atccacaaag
    51841 tccactgcat cgccatttga ggcatcttgc aattctacca gctctttgat ccagttcatg
    51901 ccaacggctt gctcagcctg attgacctta ccacgcacgc cttttttata agcccagtga
    51961 gcagcaaccc cgtactcagc cacttgatgc atgtccttag ttctgatttg aatctcaata
    52021 ggtccttttg gtccatacac agtggtatga atcgactggt agccattagc tttaggagct
    52081 gcaatataat ccttgaagcg gcctggcatg ggacgccaaa gctcatgaat atagccaacc
    52141 atagcataaa catcgctctg cgtttccatg acacaacgga tagcaatcaa atcaaaaatc
    52201 tgatcgaaac gtttcttttt gtcccgcatt ttccgataaa tcgaataaat gtgttttggt
    52261 cggccataca catctccaaa caacccttgt tgtgtcgtat aggttttgac cttactgaca
    52321 atagcctcta ccaaagcttc acgctcgcga cgtttttctt tcatcatatg ggaaattttg
    52381 taaaattcgg tttcattgag gtaacgaaaa gccaaatctt ctagttccca tttgatgcga
    52441 ctaatcccca aacgatgcgc caagggggca tagatttcca tggtttcgcg cgaaatgcgc
    52501 tcttgtttgt ccttgcgcaa atgtttgagg gtgcgcatat tatgcaggcg gtcagccaat
    52561 ttcaccaaaa tcacgcgaat atctttagac atagccatca gcattttgcg atggttttcg
    52621 gcgagttgct cctcatgaga tttgtactca actttaccta acttggtgac accatcaacg
    52681 atatcacgag catcttggcc aaagtctgct tcgatctcat ctaaggtaat atccgtatct
    52741 tctacgacat catgtaaaaa gccacaggca actgtcacag catccagatg caaatcagcc
    52801 agaatccccg ccacctgaat cggatggacg atatagggtt cgcctgactt tctcacttgg
    52861 tagaaatggg ccgctgttgc atatgctaaa gcctttgcca caaaagccac atcagtcttg
    52921 gtcatatagg tggccgctaa ggcaatgact tcttctcctg ttacgttcat tatttttgcc
    52981 atttcgttcc tcatctagca tttcttttcc ctattttaac actttacaca caaaagcaaa
    53041 aggcagaatc tgctaagaaa gcgcttagtg tgaccttttc ttagaaaacg gttagtgagc
    53101 tgcttttgat atcttttggg atctctcagg cgtgagcatg ataccaacgg ccagctcccg
    53161 accaaaaata gagagaacca aggggctctc tctattatgt ttgcttatca gtaaggtctc
    53221 tcaattcaga ggattagttg ttttctttgc gttttgtaac ggctaggaca ccagctgttg
    53281 tcatcactgc tactgctgct gctgtaaaga atgggttggc ttgctcacca gttgctggta
    53341 gacgtctgtg gttgttggct acaggtgtct tagccatatt gccattgcgg cgttctggcg
    53401 tttggttagc ttctttgtca gcaggttttt ctggagctgg agttacctct ttgctctttt
    53461 ctggagcttt ttcacctggt tgctctggca tttctggcgt tggtgtcact tctgggctct
    53521 ttt
//
LOCUS       DATXQK010000009        53415 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.009, whole genome shotgun sequence.
ACCESSION   DATXQK010000009 DATXQK010000000
VERSION     DATXQK010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 53415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53415
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.009"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            99..536
                     /locus_tag="VC394_000551"
     CDS             99..536
                     /locus_tag="VC394_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEP5994044.1"
                     /translation="MNQNISAAEWEVMRVVWASGDIKSSDIITILRKKYQWSDSTIKT
                     LIGRLVEKKALGTYRQGRAYIYQALLDETLLQKEALATVLDGICQRQHTHLLLERLYD
                     LPMTLEEIEAFQELLEVKKENAVLEVSCNCLPGQCHCQKKETY"
     gene            536..2767
                     /locus_tag="VC394_000552"
     CDS             536..2767
                     /locus_tag="VC394_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEP5994045.1"
                     /translation="MAKEIFLVEGMSCASCALTIEKMVNQLPEVEQAVVNLATEKLTV
                     TYQDDDLETAKVIQAVKAAGYGAQVFDEHAKHMQTGRRDEDLKVIWSQVLWSALFTIP
                     ILYLAMGHMVGLWLPPFLQPDRYPFVYSFSQLLLTIPVLVLNRHYYHNGFKALFKGYP
                     NMDSLVALATSFAFAYSLYGVVEISLGQANFVHSLYFESVVVILTLIGLGKYFESRSK
                     GRTSQAIQKLLSLKATVVRVWRNSQWQLLPLEEVSYDDLVLVQPGEKVSIDGIIVEGH
                     SSLDESFLTGESLPVEKGEKDNVFAGSLNGPGALTIKPDKLGNDTLLAQIIQLVENAQ
                     ENKAPIAAIADKVSGVFVPVVLVLALLTGVFWLVFMQESLRFSLTTAIAVLVIACPCA
                     LGLATPTAIMVGTGRAAENGILFKGGDILEQAHQVDTVVFDKTGTITEGKPSLQKLLT
                     FSGDDHLILQEAASLEAYSQHPLGEAIVRAAQEAGYDSLPVEEFESLTGLGVTGQLEG
                     RRLAIGNAALMGILGIDLSCVQSVCAKASEKGQTLVYYAKEGQLRALFSIADAVKEDS
                     QATVEALHQLGIHTIMLTGDHDATAKAIASQVGITDVISQVLPDQKAGVIADLRSQGR
                     KVAMVGDGINDAPALAVADIGIAMGSGTDIAIESADVILMKPDMLDLVKAMSLSRATM
                     RIVKENLFWAFIYNVLMIPVAMGLLHLFGGPLLNPMLAGFAMSFSSVSVVLNALRLKG
                     KTI"
     gene            2781..2984
                     /locus_tag="VC394_000553"
     CDS             2781..2984
                     /locus_tag="VC394_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEP5994046.1"
                     /translation="MEKHYQVTGMTCDGCARTVTEKLSAVPGVQSVQVNLEKGQAKVT
                     GRPLTFLLKRALKDTKFELHQSS"
     gene            complement(3188..3958)
                     /locus_tag="VC394_000554"
     CDS             complement(3188..3958)
                     /locus_tag="VC394_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEP5994047.1"
                     /translation="MLKRERLLKITEIVNEQGIVTVNDIIQTLNVSDMTVRRDLDELE
                     KAGKLIRIHGGAQSITMPNKKERSNIEKQTVQTKEKWELASYATQLVNDGETIFIGPG
                     TTLECFAEQLKNRQIRIVTNSLPVFNILQDSETIDLILIGGEYRSITGAFVGSLASQN
                     ISSLKFAKAFISCNGIYKNDIATYSETEGEIQKLAFNNSIEKYLLVDNQKFNAYDFFI
                     FYHLNNIDAVVTDSQITEDVIERYSQFTQLLVAHENKE"
     gene            4195..4668
                     /locus_tag="VC394_000555"
     CDS             4195..4668
                     /locus_tag="VC394_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEP5994048.1"
                     /translation="MFPNILFTEARTQPELFDLVASYLEKVGYVTQEYHQALVEREAV
                     FPTGLKVDLKDGSDILYAAIPHTETKYCLVDQVVYVRNSQALTFKHMINPEEDCLVTD
                     FFFIINSQNEGQTTILSNLITFFITKGNLSYLASLKDDKQAISNYLIEKGVFQHD"
     gene            4661..4966
                     /locus_tag="VC394_000556"
     CDS             4661..4966
                     /locus_tag="VC394_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEP5994049.1"
                     /translation="MIKILAACGAGVNSSHQIKDAIETQMSDRGYDVHCDAVMVKDIT
                     EELVSRYDIFTPIAKTDLGFEMPIPTVEAGPILYRIPIMSEPVFAELERVIKENHLQ"
     gene            4992..6443
                     /locus_tag="VC394_000557"
     CDS             4992..6443
                     /locus_tag="VC394_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEP5994050.1"
                     /translation="MMQPFLDIINKILGFPIQLGSGVVMLIVMTGLAMIFGVKFTKAL
                     EGGIKLAIALTGIGAIIGILTGAFSESLQAFVKNTGISLNIIDVGWAPLATITWGSPY
                     TLYFLLVMLVVNIVMIVMKKTDTLDVDIFDIWHLSITGLLIMWYADHNHLPVLVSLLI
                     ATVAVILVGVLKIINSDLMKPTFDDLLGTGSQSPMTSTHMNYMMNPIIMVLDKIFDKV
                     FPGLDKYDFDAAKLNKKIGFWGSKFFIGMALGFVIGIMGDPHFTVESIKNWFGLGFTA
                     GACLELFSLIGSWFIAAVEPLSQGITNFANARMHGRRFNIGLDWPFIAGRAEIWACAN
                     ILAPIMLIEAVLLSKVGNGILPLAGIIAMGMTPALLVVTRGRLIRMIIFGSLLLPLFL
                     LSGTMIAPFATELAKKVGAFPAGTSAGSLITHSTLEGPMEKIFGYVIGQATTGQIASI
                     ITLIIFVAIYLGLFAWYANQMKARNAEYAKTMK"
     gene            6700..7125
                     /gene="lacA"
                     /locus_tag="VC394_000558"
     CDS             6700..7125
                     /gene="lacA"
                     /locus_tag="VC394_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEP5994051.1"
                     /translation="MAIILGADAHGNALKELIKSFLQEEGYDIIDVTDINSDFIDNTL
                     AVAKAVNEAEGRLGIMVDAYGAGPFMVATKLKGMVAAEVSDERSAYMTRGHNNARMIT
                     IGAEIVGPELAKNIVKGFVTGPYDGGRHQIRVDMLNKMA"
     gene            7140..7655
                     /gene="lacB"
                     /locus_tag="VC394_000559"
     CDS             7140..7655
                     /gene="lacB"
                     /locus_tag="VC394_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEP5994052.1"
                     /translation="MKIAIGCDHIVTNEKMAVSDFLKSKGYDVIDCGTYDHTRTHYPI
                     FGKKVGEAVVNGQADLGVCICGTGVGINNAVNKVPGIRSALVRDMTTALYAKEELNAN
                     VIGFGGKITGELLMCDIIDAFIKAEYKETEENKKLIAKIAHLESHHANQEDPDFFTEF
                     LEKWDRGEYHD"
     gene            7665..8118
                     /locus_tag="VC394_000560"
                     /pseudo
     CDS             7665..8118
                     /locus_tag="VC394_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061423.1"
                     /note="catalyzes the formation of tagatose
                     1,6-bisphosphate from tagatose 6-phosphate and ATP;
                     frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
     gene            8165..9142
                     /gene="lacD"
                     /locus_tag="VC394_000561"
     CDS             8165..9142
                     /gene="lacD"
                     /locus_tag="VC394_000561"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEP5994053.1"
                     /translation="MTITANKRHYLEKVSHQGIISALAFDQRGALKQMMAAHQEGEAT
                     VTQIETLKVLVSEELTPYASSILLDPEYGLLATKVRANQTGLLLAYEKTGYDATTTSR
                     LPDCLVEWSVKRLKAAGADAIKFLLYYDVDGDEQINLQKQAYIERIGSECTAEDIPFF
                     LELLSYDERISDNNSAAYAKLKPHKVNGAMSVFSDKRFGVDVLKVEVPVNMAYVEGFT
                     EGEVHYSQAEAIKAFQDQEAASHLPYIYLSAGVSAKLFQETLYFAAAAGAQFSGVLCG
                     RATWAGSVPVYITKGEDEARKWLCTEGFQNIDELNRVLEETASPWTEKI"
     gene            9339..9956
                     /locus_tag="VC394_000562"
     CDS             9339..9956
                     /locus_tag="VC394_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HEP5994054.1"
                     /translation="MTIDMWEILNIIGTIAFALSGAIVAMEEEFDILGIFILGFVTAF
                     GGGAIRNTLIGLPIEALWGQKPEFTCAFFAMVLIMLFPKLIARGWVRAVVLTDAIGLA
                     AFSVQGALHAVRLNQPLSAVIVAAVLTGAGGGVVRDILAGRKPSVLRSEIYAGWSILA
                     AIVLHFKLADSTIECYALVVLLTTLRMIGNRKKWNLPKIKWKEER"
     gene            9958..10782
                     /locus_tag="VC394_000563"
     CDS             9958..10782
                     /locus_tag="VC394_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEP5994055.1"
                     /translation="MIQLIAIDLDGTLLNQDKQIPKENITAIQEAAQSGLKIVLCTGR
                     PQSGTRPYFDQLGLTQEEFLIINNGCSTYSSPDWQLRHSKMLKVSDIELLEELSQSFP
                     DIYLTLTEENDYLVLEEEVPDLVQEDGDLVFTIVKPVSLAELSDTPRLIFQAMYLGEK
                     AALDAFERAVRNQLSQSFHVVRSQDNILEILPQGVSKASALKELVEDLGLTADQVMAI
                     GDAPNDIEMLTYAGLGVAMENASAAIKPLADKVTLTNDMAGVAQAIRQFALAAQKD"
     gene            complement(10818..11375)
                     /locus_tag="VC394_000564"
     CDS             complement(10818..11375)
                     /locus_tag="VC394_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEP5994056.1"
                     /translation="MTDKALSKRSLQNLTQFNKETRRIARESMEIALLNLLETKPLGD
                     ITISELVTKAGVSRNAFYRNYTSKEAIIEQLLVGVIRRIFRGLKQFDIKTQAYQAWLY
                     LFTEAKKEAQLLKMIFKHHLHHLLTRLVTKRLKAYQKLKDKKQSHYKRLFWSNAIVSV
                     LTNWISDDMIVPAEEMAAMGLPLLT"
     gene            11668..12510
                     /locus_tag="VC394_000565"
     CDS             11668..12510
                     /locus_tag="VC394_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEP5994057.1"
                     /translation="MTWKIVTDSGCDLRSLTRQSKELRFERVPLTLQIGTEIFRDDDG
                     LDIDNMMTTMYQSSKATTSSCPSPEAFLQAYRGADNVIVMTITGTLSGSHNSARLAKN
                     ELLEENPNVNIHLIDSLSAGGEMDLLVLELERLINLGLSFEEVVKQITAYQQKTRLIF
                     VLAKVDNLVKNGRLSKLVGKVIGLLNIRMVGKASNKGTLELLQKARGQKKAVSALIEE
                     IQKEGYVGGKVYIAHAQNPKICEQISEKIKSLYPDAVIQTGRTSGLCSFYAEDGGLLM
                     GYEI"
     gene            complement(12639..13361)
                     /locus_tag="VC394_000566"
     CDS             complement(12639..13361)
                     /locus_tag="VC394_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994058.1"
                     /translation="MATMKQYHKGDSMTKSQKEALYWMLSVLTITLIGGSCLIFGSHP
                     QTQDKVAKHSKSAASLLKKAVKAVNDADRLATAAAIQEAQKAVDKLAESSKKKTLQEQ
                     LNVAKAKQEQEDAATQAVKAAEETLNQNLKDIAQKAVNDLSNKGKKAALQSRLDAILP
                     AKPIIDEFPRQSGEITDNSYWTPFPGDVSDTYDNSQSPTLDPSSESSASDVTPQPSHP
                     DPIPPQTSSEPSDSGDKQSSKE"
     gene            13563..15194
                     /locus_tag="VC394_000567"
     CDS             13563..15194
                     /locus_tag="VC394_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEP5994059.1"
                     /translation="MSVNWQDIAFHFFGGLGLFLFSIKYMGDGLQQAAGDKLRYYIDK
                     YTSNPFFGILVGIAMSALIQSSSGVTVITVGLVSAGLLNLRQAIGIVMGANIGTTITS
                     FLIGFKLGDYALPMIFIGAACLFFTSNKKLNNFGRIIFGVGGIFFSLNLMGDAMDPLK
                     SVSAFQNYLATLGDKLFQGVFIGTALTMLIQSSAAIIGILQGLFSGGLLTLQGAIPIL
                     LGSNIGTCITAVLAAIGSNIAAKRVAAAHVLFNLIGTIIFMIILVPFTSLMLWLQSKL
                     SLTPEMTIAFSHGSFNITNTILLIPFISLLAMIVTRLIPGEDEVVKYEALYLDRLLIT
                     QAPSIALGNAHKELVHLASYAIQAFEASYSYIMTADGKFGEKVKRYERAVDTIDEELT
                     TYLVDISNEALSPSENEVLAGILDSSRDLERIGDHSESLGILIEGIISKQIGFSISAR
                     QELTEMYQLTHCLTLDAIRAIVDSDTDLAQTIVTRHKEIEEKERRLRKTHIKRLNCGE
                     CTAQAGINFIDIISHYTRITDHALNLAEKVLSHQL"
     gene            15311..16459
                     /gene="nagA"
                     /locus_tag="VC394_000568"
     CDS             15311..16459
                     /gene="nagA"
                     /locus_tag="VC394_000568"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEP5994060.1"
                     /translation="MTCYLKADCFYYPTEVKPAGYLGLHDGVFGEWTEIVPADAQIID
                     YTGYQIAPGLVDTHIHGYAGADVMDNSAQGIHQMSEGLLATGVTSFLPTTLTSTFEQL
                     EKVSGTIASVADQVKGAKIQGIYFEGPYFTEEYKGAQNPSYMKTPRLEEFDAWQKAAK
                     GLIKKIALAPEREGVKEFVSAVTKQGVTVALGHSNGTYQEAKEAVQAGASVWVHAYNG
                     MRGLTHREPGMVGAVYNLPNTYAELICDGHHVSPIACDILMQQKGHDHVAMITDCMRA
                     GGSPDGDYLLGEFSVVVANGTARLKESGNLAGSILKLKDGLRNVVAWGIATPAEAIHM
                     ATYVPAVSVGIDDVCGQIKAGHAADFIVLDKDLTLVATYLNGQKAFDA"
     gene            16581..17420
                     /locus_tag="VC394_000569"
     CDS             16581..17420
                     /locus_tag="VC394_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEP5994061.1"
                     /translation="MVTTVKMTSGYEIPVLGFGTYQAADGEEAYQSTLAAIKAGYRHI
                     DTAAIYKNEESVGRAIKDSGVLREDLFITTKLWNDAHSYEGAKDALAASLDRLGLDYV
                     DLYLIHWPNPKALRNTWKEANAQAWQYMEEAVEAGLIKSIGVSNFMVHHLEALQETAK
                     ITPAINQIRLAPGCYQKEVVDYCKANEILLEAWSPLGQGEIFDNETMQQLANKYDKTV
                     AQVALAWSLAEGFIPLPKSVHDERIKENMAIFDVSLTQEDKKTIRYLSGMSAIPNPDT
                     TSF"
     gene            17552..18085
                     /locus_tag="VC394_000570"
     CDS             17552..18085
                     /locus_tag="VC394_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEP5994062.1"
                     /translation="MTIFLVMLAMFTIRLVFLKKSITNEKAILAQGGQEFGAQNTKFL
                     TLLHIMIYVFAVIEALLKQIKFDGISFLGLLLMLLSVAVLYEVTRILGDIWTVKLMLA
                     KDHKYVDHWLFKTIKHPNYFLNIAPELVGIALLCHAKITAMLLFPCYIVVIYLRIREE
                     NKLLAEVIIPNGTRTKP"
     gene            18457..19374
                     /gene="glyQ"
                     /locus_tag="VC394_000571"
     CDS             18457..19374
                     /gene="glyQ"
                     /locus_tag="VC394_000571"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEP5994063.1"
                     /translation="MSKKLTFQEIILTLQQYWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLASLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATSPVTAE
                     VTYGLERLASYIQEVDSVYDIEWAPGVKYGEIFLQPEYEHSKYSFEISDQDMLLENFE
                     KFEKEASRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKKLGFPLLDEATRAILLAEDDE"
     gene            19752..21791
                     /locus_tag="VC394_000572"
     CDS             19752..21791
                     /locus_tag="VC394_000572"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEP5994064.1"
                     /translation="MSKNLLIELGLEELPAYVVTPSEKQLGERLATFLTENRLSFEDI
                     QTFSTPRRLAVRVSGLADQQTDLTEDFKGPAKKIALDADGNFSKAAQGFVRGKGLTTD
                     AIEFREVKGEEYVYVTKHEAGKPAKEVLLGVTEVLSAMTFPVSMHWANNSFEYIRPVH
                     TLTVLLNDEALELDFLDIHSGRVSRGHRFLGTETTITSADSYEADLRSQCVIVDAKER
                     QEMIVEQIKTLEVEQGVQVDIDEDLLNEVLNLVEFPTAFMGNFEAKYLDVPEEVLVTS
                     MKNHQRYFVVRDQAGHLMPNFVSVRNGNDQAIENVIKGNEKVLVARLEDGEFFWREDQ
                     KLQIADLVAKLTNVTFHEKIGSLAEHMDRTRVIAASLAKEANLSAEEVTAVDRAAQIY
                     KFDLLTGMVGEFDELQGIMGEKYALLAGEDAAVATAIREHYLPDAAGGALPETKVGAV
                     LALADKLDTLLSFFSVGLIPSGSNDPYALRRATQGIVRILDHFGWRIPMDKLVDSLYD
                     LSFDSLTYTNKADVMNFIRARVDKMMGKAAPKDIREAILASSTFVVPEMLAVAEALVK
                     ASHTENYKPAVESLSRAFNLAEKADASVQVDPSLFENEQENTLFAAIQGLTLAGSAAQ
                     QLEQVFALSPVINDFFDNTMVMAEDQALKNNRVAILSDLVSKAKTIAAFNQLNTK"
     gene            21945..22202
                     /locus_tag="VC394_000573"
     CDS             21945..22202
                     /locus_tag="VC394_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEP5994065.1"
                     /translation="MDPKKIARINELAKKKKTVGLTGPEKVEQAKLREEYIEGYRRSV
                     RHHIEGIKLVDEEGNDVTPEKLRQVQREKGLHGRSLDDPKS"
     gene            22329..22721
                     /locus_tag="VC394_000574"
     CDS             22329..22721
                     /locus_tag="VC394_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEP5994066.1"
                     /translation="MCLSYNRRNQRKKVVLMTIKKFSLLAIASLSLLSLAACDMDDKD
                     DHMDNQPKTSQTSKKVKLSEDKAKSIALKDASVTEADAQMLSVTQDNEDGKAVYEIEF
                     QNKDQEYSYTIDANSGDIVEKSSEPIND"
     gene            23081..24607
                     /gene="glpK"
                     /locus_tag="VC394_000575"
     CDS             23081..24607
                     /gene="glpK"
                     /locus_tag="VC394_000575"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEP5994067.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNQKGEKVSSSQKEFPQIFPHAG
                     WVEHNANQIWNSVQSVIAGAFIESSIKPSQIEAIGITNQRETTVVWDKKTGVPIYNAI
                     VWQSRQTAPIAEQLKQDGHTKMIHEKTGLVIDAYFSATKIRWILDHVPGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAVHVTDYSNAARTMLYNIKDLTWDDEILELLNIPKDMLPEV
                     KSNSEIYGKTAAFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GDEMQLSSNNLLTTIGYGINGKVHYALEGSIFIAGSAIQWLRDGLKMIETSPESEQLA
                     LASTSDDEVYVVPAFTGLGAPYWDSNARGSVFGLTRGTSKEDFVKATLQSIAYQVRDV
                     IDTMQVDSGIDIQQLRVDGGAAMNNMLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     AVGYWEDMDALKELNATGQLFKASMNESRKEKLYKGWKRAVKATQVFTQEEDADDDAK
                     "
     gene            24623..26461
                     /gene="glpO"
                     /locus_tag="VC394_000576"
     CDS             24623..26461
                     /gene="glpO"
                     /locus_tag="VC394_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEP5994068.1"
                     /translation="MEFSRETRRLALQKMQERDLDLLIIGGGITGAGVALQAAASGLD
                     TGLIEMQDFAQGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEPGSTFSMFRLKVAMDLYDLLAGVSNTPAANKVLTKEEVLKREPDLKQEGL
                     LGGGVYLDFRNNDARLVIENIKRANRDGALIASHVKAEDFLLDDNGKIIGVKARDLLS
                     DQEIIIKAKLVINTTGPWSDETRQFSHKGQPIHQMRPTKGVHLVVDRQKLPVSQPVYV
                     DTGLNDGRMVFVLPREEKTYFGTTDTDYTGDLEHPQVTQEDVDYLLGVVNNRFPNANV
                     TIDDIESSWAGLRPLLSGNSASDYNGGNSGKVSDDSFDHLVDTVKAYINHEDSREAVE
                     KAIKQVETSTSEKELDPSAVSRGSSFDRDENGLFTLAGGKITDYRKMAEGALTGIIQI
                     LKEEFGKSFKLINSKTYPVSGGEINPANVDSEIEAYAQLGTLSGLSMDDARYLANLYG
                     SNAPKVFALTRQLTAAEGLSLAETLSLHYAMDYEMALKPTDYFLRRTNHLLFMRDSLD
                     ALIDPVINEMAKHFEWSDQERVAQEDDLRRVIADNDLSALKGQQEG"
     gene            26463..27164
                     /locus_tag="VC394_000577"
     CDS             26463..27164
                     /locus_tag="VC394_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEP5994069.1"
                     /translation="MDIFGEFLGTALLVLLGNGVVAGVVLPKTKTHASGWIVIATGWG
                     IAVAVAVFISGKVAPAHLNPAVSLAFAMSGTIAWSTAIAYSLAQLLGAMVGSTLVFLQ
                     FRPHYLAAESQADILGTFATGPAIRDTSSNLLSEIFGTFVLMLGILAFGLYDMPAGLG
                     TLCVGTLVIGIGLSLGGTTGYAINPARDLGPRLVHAILPLNNKGDSDWSYAWIPVVGP
                     IIGAALAVLLFQVMS"
     gene            27273..28619
                     /locus_tag="VC394_000578"
     CDS             27273..28619
                     /locus_tag="VC394_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEP5994070.1"
                     /translation="MTLTTKTIHVIGASFAGLAFVDKYKDLNPDSQIILIDKESCPNY
                     IPNGINQLFRGDIQDLSDAMWGRACLAAQIESNHRFIQAEVLAIEAPSNTLLLKDSQG
                     RVFEEGYETLVCAMGASPQSHYIETSQTNKVLVTKYYEESQASLKLIEASQEVLVIGA
                     GLIGLDLAYSLSLQGKRVKLIEAAERPDFYQTDAELIAPVMAEMSTHHVTFINNKRVT
                     AIHEIEGKVVAHTEQGDTFQGDLAILAINFRPNTHLLQGQVACALDKTILVNENLQTS
                     QANIYAIGDMVSLYFGILGMDYYTPLINQAMKTGQALALHLAGYPIPPLQTVKVLGSS
                     HFGYYRASVGVTEEEAELYMDTCSYLYQNGDSKNLFWLKLIARKTDGILIGAQLLSKT
                     NALVIANQLGQALALKVTDGDLAFQDFLFLQGHSDLAYHLHEACLKLFEKRLRHED"
     gene            28609..30108
                     /locus_tag="VC394_000579"
     CDS             28609..30108
                     /locus_tag="VC394_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEP5994071.1"
                     /translation="MKIEDLMDKERKAQYRLLVTLYHAKETLRLKDLMRLSNLSKVTL
                     LKYIDNLNHLCREQGLACQLLLEKDSLSLKENGQFHWEDLVALLLKESVAYQILTYMY
                     CHEHFNITNLSVELMVSEATLNRQLAHLNQLLSEFDLALSQGRQLGSELQWRYFYLEL
                     FRHTLTRQGIDALVNQLDASHLATLIERLIGQSLSAEALEQLLIWLAISQARMSFQKG
                     YNDHFLRDSDFMTSNIFFKRLESMLLHYLRRYALEFDAFEAKSLFVFLHAYPLLPIAS
                     MKYSLGFGGPIADHISEALWLLKKAHVIIHQTKEEIIYGLGIFFSKAYFFKGAILSQP
                     TNSQYLYQLVGEDKRALLRVIINHLVLQMGQETDFSQQLSDDILALLVFSIERHHEPL
                     LVGLALGQNKVEAAIAELAIRRHLGHRRDFQLMPYDHQKVYDCLITYQTVYLPRQDLP
                     YYRLKQYSSPYELTALEAFLKDLFQQKNTREEELSLSPAAKSSFAHKTV"
     gene            30234..30878
                     /gene="fsa"
                     /locus_tag="VC394_000580"
     CDS             30234..30878
                     /gene="fsa"
                     /locus_tag="VC394_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEP5994072.1"
                     /translation="MKFFLDTANVAAIKAINELGVVDGVTTNPTIISREGRDFETVIK
                     EICDIVDGPISAEVTGLTADAMVEEARSIAKWHDNVVVKIPMTTEGLKATNILSKEGI
                     KTNVTLIFTVSQGLMAMKAGATYISPFIGRLEDIGTDAYQLISDLREIIDLYDFQAEI
                     IAASIRTTAHVEAVAKLGAHIATIPDPLFAKMTQHPLTTNGLKTFMEDWASFKK"
     gene            31096..33081
                     /gene="tkt"
                     /locus_tag="VC394_000581"
     CDS             31096..33081
                     /gene="tkt"
                     /locus_tag="VC394_000581"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013793690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEP5994073.1"
                     /translation="MTFDAIDQLAVNTVRTLSMDAIQAANSGHPGLPMGAAPMAYVLW
                     NHFMNINPKTSRNWSNRDRFILSAGHGSAMLYSLLHLAGYDLSVEDLKNFRQWGSKTP
                     GHPEVNHTDGVEATTGPLGQGIANAVGMAMAEAHLAAKFNKPGFDIVNHYTFALNGDG
                     DLMEGVSQEAASMAGHLKLGKLVLLYDSNDISLDGPTSMAFTEDVKGRFEAYGWQHIL
                     VKDGNDLEEIAAAIEAAKAETEKPTIIEVKTIIGFGAEKQGTSAVHGAPLGAEGIAFA
                     KKAYQWTHQDFEVPAEVTERFAQGLQARGEKAEQAWNDLFAVYEAEYPELAAEYQKAF
                     TNEAAQVELEAHELGSSMASRVSSQQAIQQISEQVASFWGGSADLSASNNTMVKAETD
                     FQPGHYEGRNIWFGVREFAMAAAMNGIALHGGTRVYGGTFFVFSNYLLPAVRMAALQN
                     LPTVYVMTHDSIAVGEDGPTHEPIEQLASVRSMPNFNVIRPADGNETNAAWKRAIAET
                     DRPTMLVLTRQNLPVLEGTKELAEDGLNKGAYILSEAKGDLEGILIATGSEVKLAMDT
                     QEALEAEGIHVRVVSMPSQNIFDEQSAEYKESILPAAVTKRLAIEAGSSFGWAKYVGL
                     AGKTLTIDTWGASAPGNRIFEEYGFTVANATELYKSL"
     gene            33274..33540
                     /locus_tag="VC394_000582"
     CDS             33274..33540
                     /locus_tag="VC394_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEP5994074.1"
                     /translation="MEKKRQRLYDVIRQAYDYPENRENVALSQLLLAASNRLIKHSNP
                     LLVAYQLNQDVDNYLLDNDILLPKSLCRFKQSLEKYILEEASSV"
     gene            33574..34308
                     /locus_tag="VC394_000583"
     CDS             33574..34308
                     /locus_tag="VC394_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5994075.1"
                     /translation="MIEFKHVSKLYGDKEALSDLNVTINDGEIFGLIGHNGAGKTTTI
                     SILTSIIEASYGEVFVDGQLLTENREAIKKQIAYVPDSPDIFLNLTPNEYWQFLAKIY
                     GVSDEDREERLAQLTTLFELKEEVNQTIDSFSHGMRQKVIVIGALVSNPNIWILDEPL
                     TGLDPQASFDLKEMMKAHAASGHTVLFSTHVLSVAEQLCDRIGILKKGKLIFVGTIDE
                     LKEHHPDKDLESIYLELAGRKAQEEG"
     gene            34313..35941
                     /locus_tag="VC394_000584"
     CDS             34313..35941
                     /locus_tag="VC394_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP5994076.1"
                     /translation="MNWSTIWELIKINILYSNPQSLANLKKRQENHPKENFKAYKSMM
                     RQQALMIAMFLVIYLFMFIGVDFSHYPGLFSFDVAMFFIMSTLTAFSSLYTIFYESND
                     LKLYIHLPVTSEELYIAKIVSSLGMGAVFLMPLISLLLIAYWQLLGNPLSILVAIVLF
                     LVLLVSSMVLAIYINAWVGKIIVRSRKRKLISTIMMFVSTFGAFVLIFAINISNNKRT
                     MTDGVFTDYPTIPYFKGFYDVIQAPFSTAALLNFWLPLLLILAMVYGIVTKVMPTYYR
                     EAFYISNENKAKQTKKPVNRPHQNQSLTQLLRKHHLLTLQNATLLTQTYLMPLMYVML
                     FIGPSLSRGTGFFKHISPDYFGVALLFGVSLGVMCATPTSFIGVGISLEKDNFTFIKS
                     LPITLKKFLLDKFCLLVGLQLIVPMVIYLVFGLFVLHLHPLLTIAFCLGYALSLIVQG
                     ELMYRRDYRLLDLKWQDMTQLFTRGNGQWLTMGLIFGNLIVTGVVGFGAVIIANIIQQ
                     PLLISILLSCLTLMVLGIAHLWIQKTFWKSLEKL"
     gene            36005..36826
                     /gene="proB"
                     /locus_tag="VC394_000585"
     CDS             36005..36826
                     /gene="proB"
                     /locus_tag="VC394_000585"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEP5994077.1"
                     /translation="MMKRQFEDVTRIVIKIGTSSLVLPTGKINLEKIDQLAFVISSLM
                     NKGKEVILVSSGAMGFGLDILKMEKRPTNLAKQQAVSSVGQVAMMSLYSQIFAHYQTN
                     VSQILLTRDVVVFPESLANVTNAFESLISLGIVPIVNENDAVSVDEMDHATKFGDNDR
                     LSAVVAGITKADLLIMLSDIDGLFDKNPTIYEDAQLRSHVAVITQEIIASAGGAGSKF
                     GTGGMLSKIQSAQMVFENKGQMVLMNGANPRDILRVLEGQPLGTWFKQIEEVTHD"
     gene            36819..38069
                     /locus_tag="VC394_000586"
     CDS             36819..38069
                     /locus_tag="VC394_000586"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEP5994078.1"
                     /translation="MTDMRRLGQRAKQASLLIAPLSTQIKNRFLSTLAKALVDDTQTL
                     LAANQKDLANAKEHGISDIMMDRLRLTSERIKAIAQGVQQVADLADPIGQVIKGYTNL
                     DGLKILQKRVPLGVIAMIFESRPNVSVDAFSLAFKTNNAIILRGGKDALHSNKALVKL
                     IRQSLEKSGITPDAVQLVEDPSHAVAEELMQATDYVDVLIPRGGAKLIQTVKEKAKVP
                     VIETGVGNVHIYVDAQADLDMATNIVINAKTKRPSVCNAAEGLVIHEAVAARFIPMLE
                     KAINQVQPVEWRADDKALPLFEQAVPAKAEDFETEFLDYIMSVKVVSSLEEAISWINQ
                     HTSHHSEAIITRDIKAAETFQDLVDAAAVYVNASTRFTDGFVFGLGAEIGISTQKMHA
                     RGPMGLEALTSTKFYINGDGHIRE"
     gene            38089..38310
                     /locus_tag="VC394_000587"
     CDS             38089..38310
                     /locus_tag="VC394_000587"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994079.1"
                     /translation="MTNEREKEEAKVALEELLHQIQVIHLELKIVRNWGYFDLFGDGM
                     LVSWIKRRKMSKVNQLLIDLQVTLQNCRR"
     gene            38353..38517
                     /locus_tag="VC394_000588"
     CDS             38353..38517
                     /locus_tag="VC394_000588"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994080.1"
                     /translation="MSWDKIFDIWLDNLLTDCCVIKEAKNFLQVVEILEAAAKSVLAK
                     LADSEQKEQD"
     gene            38541..39554
                     /gene="rsmH"
                     /locus_tag="VC394_000589"
     CDS             38541..39554
                     /gene="rsmH"
                     /locus_tag="VC394_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEP5994081.1"
                     /translation="MKSFSLTFSFLNLLKYGTIKVMTKEFHHVTVLLHETVDMLDIKP
                     DGIYVDATLGGSGHSAYLLSKLGEEGHLYCFDQDQKAIDNAQVTLKSYIDKGQVTFIK
                     DNFRHLKARLTALGVDEIDGILYDLGVSSPQLDERERGFSYKQDAPLDMRMDRQSLLT
                     AYEVVNTYPFNDLVKIFFKYGEDKFSKQIARKIEQARAIKPIETTTELAELIKAAKPA
                     KELKKKGHPAKQIFQAIRIEVNDELGAADESIQDAMELLALDGRISVITFHSLEDRLT
                     KQLFKEASTVDVPKGLPLIPEDMKPKFELVSRKPILPSHSELTANKRAHSAKLRVAKK
                     IRK"
     gene            39559..39882
                     /gene="ftsL"
                     /locus_tag="VC394_000590"
     CDS             39559..39882
                     /gene="ftsL"
                     /locus_tag="VC394_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEP5994082.1"
                     /translation="MTNEKRTQAVTNALQKRIKTFSRIEKAFYTAIIVTAITMAVSII
                     YLQSRKLQLQQEITSLNSHISDQKLELNNAKQEVNELSRRDRIIDIAGKAGLSNRNNN
                     IKKVE"
     gene            39886..42141
                     /locus_tag="VC394_000591"
     CDS             39886..42141
                     /locus_tag="VC394_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEP5994083.1"
                     /translation="MKKWQKYVLDYVVRDRRTPVENRVRVGQNMMLLTIFIFFIFIIN
                     FMIIIGTDQKFGVSLSEGAKKVYQETVTIQAKRGTIYDRNGTAIAVDSTTYSIYAILD
                     KSFVSASDEKLYVQPSQYETVADILKKHLGMKKTDVIKQLKRKGLFQVSFGPSGSGIS
                     YSTMSTIQKAMEDAKIKGIAFTTSPGRMYPNGTFASEFIGLASLTEDKKTGVKSLVGK
                     TGLEASFDKILSGQDGVITYQKDRNGTTLLGTGKTVKKAIDGKDIYTTLSEPIQTFLE
                     TQMDVFQAKSNGQLASATLVNAKTGEILATTQRPTYNADTLKGLENTNYKWYSALHQG
                     NFEPGSTMKVMTLAAAIDDKVFNPNETFSNANGLTIADATIQDWSINEGISTGQYMNY
                     AQGFAFSSNVGMTKLEQKMGNAKWMNYLTKFRFGFPTRFGLKDEDAGIFPSDNIVTQA
                     MSAFGQGISVTQIQMLRAFTAISNNGEMLEPQFISQIYDPNTASFRTANKEIVGKPVS
                     KKAASETRQYMIGVGTDPEFGTLYSKTFGPIIKVGDLPVAVKSGTAQIGSEDGSGYQD
                     GGLTNYVYSVVAMVPADKPDFLMYVTMTKPQHFGPLFWQDVVNPVLEEAYLMQDTLTK
                     PVVSDANRQTTYKLPNFVGKNPGETSSELRRNLVQPVVLGTGSKIKKVSHQPGQTLTE
                     NQQVLILSDRFVEVPDMYGWTKSNVKTFAKWTGIDISFKGTDSGRVMKQSVDVGKSLK
                     KIKKMTITLGD"
     gene            42143..43153
                     /locus_tag="VC394_000592"
     CDS             42143..43153
                     /locus_tag="VC394_000592"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEP5994084.1"
                     /translation="MFLTLIAAIISFMVSAFTMPYFIKFYQLKKIGGQQMHEDVKQHL
                     AKAGTPTMGGTVFLLVATAVSLLVSLFSIKNTQSLALISGILSIVVIYGIIGFLDDFL
                     KIFKQINEGLTAKQKLALQLVGGLMFYFLHVSPSGISSINVFGYQLPLGIFYLFFVLF
                     WVVGFSNAVNLTDGIDGLASISVVISLVTYGVIAYVQSQFDVLLLIGAMIGALLGFFC
                     FNHKPAKVFMGDVGSLALGAMLAAISIALRQEWTLLIIGIVYVLETSSVMLQVSYFKY
                     TKKKYGEGRRIFRMTPFHHHLELGGLSGKGKKWSEWQVDAFLWGVGSLASLLVLAILY
                     VF"
     gene            43311..44654
                     /locus_tag="VC394_000593"
     CDS             43311..44654
                     /locus_tag="VC394_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEP5994085.1"
                     /translation="MSFKDYHFKQYVQQALEEIGFVNPTEVQKRLIPIVNSGRDLVGE
                     SKTGSGKTHTFLLPIFEKLDEAKAEVQVVITAPSRELATQIFDACKQIAKHFQEEIRL
                     ANYVGGTDKLRQIEKLKGSQPHIVIGTPGRIYDLVKSGDLAIHKATTFVVDEADMTMD
                     MGFLDTVDKIAASLPKSVQILVFSATIPQKLQPFLKKYLTNPVIEQIKTKTVIADTID
                     NWLVSTKGRDKNGQLLEILKTMQPYMAMLFVNTKERADDLHAFLTANGLKVAKIHGGI
                     PPRERKRIMNQVKKLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGMAGTAITLYQPSDDSDIKELEKMGIAFTPKVLKNGEFQDTYDRDRRQNREKAYQK
                     LDTEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKERRAENRAKGRAERNAKKQHF"
     gene            44849..45652
                     /locus_tag="VC394_000594"
     CDS             44849..45652
                     /locus_tag="VC394_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEP5994086.1"
                     /translation="MTSVFLTSGWAFYDYLISPIPHGKLFSWHAVFDAIPNIIQRLPI
                     TLGLTLSGATFGLVLALIFALVKINKVKLLYPIQAIFVSFLRGTPILVQLMLTYYGIP
                     LFLKFLNQKYGFDWNVNAIPASIFAITAFAFNEAAYASETIRAAILSVDTGEIEAAKS
                     LGMTSVQVYRRVIIPNATVVAIPTLINGLIGLTKGTSLAFNAGIVEMFAQAQILGGSD
                     YRYFERYISVALVYWSISILMEQVGRLIENKMAIKAPEQARNEKLGELR"
     gene            45652..46395
                     /locus_tag="VC394_000595"
     CDS             45652..46395
                     /locus_tag="VC394_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEP5994087.1"
                     /translation="MITIRNLSKTFSGQKVLDSLALDIEKGQVIALVGASGAGKSTFL
                     RSLNYLEKPDSGSISIGDFTVDFETITTEQVLILRRKLAMVFQQFNLFERRTALENVK
                     EGLKVVKKLSDQEATKLAQAELAKVGLADRKHHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSITDAAKSGQTMVLVSHDMNFVYQVADRVLFLDQGKIL
                     EQGTPEEVFRHPQKERTKEFFASYSKTYI"
     gene            46499..46723
                     /locus_tag="VC394_000596"
     CDS             46499..46723
                     /locus_tag="VC394_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEP5994088.1"
                     /translation="MLVEIFSLYVKGLILSSLTMILICLIWTLWRAKTKRDKTLIERQ
                     AFLYEMLMVAILTIPILSFAFMSILVVIKS"
     gene            46787..47704
                     /gene="trxB"
                     /locus_tag="VC394_000597"
     CDS             46787..47704
                     /gene="trxB"
                     /locus_tag="VC394_000597"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEP5994089.1"
                     /translation="MYDTLIIGSGPAGMTAALYAARSNLSVAIIEQGAPGGQMNNTFD
                     IENYPGYDHISGPELAMKMYEPLEKFNVENIYGIVQKIENFGDYKCVLTEDASYEAKT
                     VIIATGAKYRVLGVPGEEYYTSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEAIYLT
                     QFAKKVTVVHRRDQLRAQKILQDRAFANDKVDFIWDSVVKEIQGNDIKVSNVLIENVK
                     TGQVTDHAFGGVFIYVGMNPVTGMVKDLEITDQEGWIITDDHMRTSIPGIFAIGDVRQ
                     KDLRQITTAVGDGAIAGQGVYHYLESFPS"
     gene            47873..49252
                     /locus_tag="VC394_000598"
     CDS             47873..49252
                     /locus_tag="VC394_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEP5994090.1"
                     /translation="MSIKEQTDNNELENGMVRGLENRHVQLIAIAGTIGTGLFLGAGR
                     SIALTGPSIIFVYMITGAFMFMMMRAIGEMLYYDPDQHTFINFISKYIGPGWGYFSGL
                     SYWISLIFIGMAEITAVGAYVQFWFPSWPAWLIQLVFLVLLSSINLIAVRVFGETEFW
                     FAMIKILAILALIATAIFMVLTGFETHTGHASLSNIFDHFSMFPNGKLKFFMAFQMVF
                     FAYQAIEFVGITTSETANPRKVLPKAIQEIPTRIVIFYVGALVSIMAIVPWHQLPVDE
                     SPFVMVFKLIGIKWAAALINFVVLTSAASALNSTLYSTGRHLYQIANETPNALTNRLK
                     INTLSRQGVPSRAIIASAVVVGISALINILPGVADAFSLITASSSGVYIAIYALTMIA
                     HWKYRQSKDFMADGYLMPKYKVTTPLTLAFFAFVFISLFLQESTYIGAIGATIWIIVF
                     GIYSNVKFK"
     gene            49423..50877
                     /locus_tag="VC394_000599"
     CDS             49423..50877
                     /locus_tag="VC394_000599"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEP5994091.1"
                     /translation="MYKDDSLTLHTDLYQINMMQVYFEQGIHNRHAVFEVYFRKEPFN
                     NGYAVFAGLQRMVEYLEQFQFSETDLAYLEELGYPGNFLTYLKELRLELTIRSAKEGD
                     LVFANEPIVQVEGPLGQCQLVETALLNIVNFQTLIATKAARIRSVIEDEPLLEFGTRR
                     AQELDAAIWGTRAAMIGGADATSNVRAGKRFDIPVSGTHAHALVQAYGNDYDAFMAYA
                     KTHKDCVFLVDTYDTLKVGVPTAIRVAKEMGDKINFLGVRLDSGDLAYLSKTVRQQLD
                     DAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVSIE
                     QEDGSMRDTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITFTDINVNELTEIEM
                     FHPTYTYIKKTVRNFDAIPLLVDIFVKGELVYQLPTLSEIKTYAKKEFDKLWDEYKRV
                     LNPQDYPVDLARDVWQNKMTLIDNIRKDTHGKSE"
     gene            50879..51703
                     /gene="nadE"
                     /locus_tag="VC394_000600"
     CDS             50879..51703
                     /gene="nadE"
                     /locus_tag="VC394_000600"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEP5994092.1"
                     /translation="MTLQEEIIRQLGVKASIDPQEEIRKTVDFLKAYLRKHSFLKTYV
                     LGISGGQDSTLAGKLAQMAIAELREETSDQAYQFIAVRLPYGVQADEADAQKALAFIA
                     PDQTLTINIKAAVDGQVEALQAAGVEISDFNKGNIKARQRMISQYAIAGQMAGAVIGT
                     DHAAENITGFFTKFGDGGADILPLFRLNKRQGKALLKVLGADAALYEKVPTADLEDQK
                     PGLADEVALGVTYQDIDDYLEGKLISKAAQATIEKWWHKGQHKRHLPITIFDDFWK"
     gene            51888..53225
                     /gene="pepC"
                     /locus_tag="VC394_000601"
     CDS             51888..53225
                     /gene="pepC"
                     /locus_tag="VC394_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEP5994093.1"
                     /translation="MSALTETFTEQLFAHYEANAKFSAIENAVTHNGLLKSLETRQSE
                     VDNDFVFSIDLTKDKVSNQKASGRCWMFAALNTFRHKLITEFKLENFELSQAHTFFWD
                     KYEKANWFMEQVIATADQELTSRKVKFLLDVPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESISSSNSRELNQYLNKLLRQDAQILRDLIASGAKADQVEDRKAELLQEIFNFLAMTL
                     GLPPRHFDFAYRDKDDHYHVEKGLTPQAFYDKFVGLKLSDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSREVRYLNLDMKRFKELAIKQMQAGESVWFGSDVGQVSDRQKGILATN
                     TYDFEASMDINLSQDKAGRLDYSESLMTHAMVLTGVDLDETGKPLKWKVENSWGEKVG
                     DKGYFVASDAWMDEYTYQIVVRKEFLTADELAAYEKEPQVLAPWDPMGALAQC"
BASE COUNT        16035 a         9627 c        11676 g        16077 t
ORIGIN      
        1 acttaagaaa taaaatcatt agaaacttta tatatttaca aatgtagata aatatgatat
       61 aatatatcta caaatgtaga ttaaaaggtt gtgagttgat gaatcaaaat atttcagctg
      121 ccgaatggga agtcatgcgt gtggtgtggg ccagtggtga tattaaaagt agtgatatta
      181 ttactatttt aaggaaaaaa tatcaatggt cagattctac cattaaaacc ttgattgggc
      241 gtttggtgga aaaaaaggct ttgggaactt accggcaagg aagagcttat atttatcaag
      301 ctcttttgga tgagactttg ctacaaaaag aagcactagc gactgtttta gacggtattt
      361 gtcagcggca acacacgcat ctacttcttg aacggcttta tgacttaccg atgaccttag
      421 aggaaattga ggcattccaa gagctactag aagtcaaaaa agaaaatgca gttcttgagg
      481 tgtcgtgtaa ttgcctgcct gggcagtgcc actgtcagaa aaaggagacg tattaatggc
      541 taaagagata tttttagtgg agggaatgag ttgtgcctct tgtgctctaa caattgaaaa
      601 aatggtcaat caattgcctg aagtagaaca ggctgttgtg aatttagcga ccgagaaact
      661 cacggttact tatcaagatg acgatttaga aacggccaaa gttattcaag cggtaaaagc
      721 tgctggttat ggtgcacaag tctttgatga acatgcaaaa cacatgcaaa caggtcgtcg
      781 tgacgaagac ctaaaggtta tttggtctca ggtcttgtgg tctgccctat tcaccattcc
      841 tatattatac ctagctatgg gtcacatggt gggcttatgg cttcctccat ttctgcaacc
      901 agatcgctat cctttcgttt acagctttag tcagctactc ttgacgattc cagtcttagt
      961 attaaatcgt cattattacc ataatgggtt taaagctttg tttaagggat atcctaatat
     1021 ggactccttg gtagctttag caaccagctt tgcttttgct tatagtcttt atggagtggt
     1081 tgaaatcagt ttgggccagg ccaactttgt gcactccctt tattttgaat cggtggtagt
     1141 gattttgaca ctcattggtc taggaaagta ttttgaaagt cgttctaaag gccgtacttc
     1201 ccaagctatt caaaagctct tatccttgaa ggctactgtt gtgagggtat ggagaaatag
     1261 ccaatggcaa ttgttgccgt tagaagaggt tagctatgat gaccttgtcc tggttcaacc
     1321 aggtgaaaaa gtttctattg acggtattat cgtggaagga catagcagtt tggatgaatc
     1381 ctttttaact ggggaaagtt tgcctgttga aaagggagaa aaagataatg tctttgcggg
     1441 cagcttgaat gggccaggcg ccctcacgat taaaccagat aagcttggga atgatacctt
     1501 gttagcacaa attatccaac tagttgagaa tgctcaagaa aataaagcac ctattgcagc
     1561 tattgctgat aaggtctcag gtgtctttgt tccggtggtt ttggtattag ctctgttaac
     1621 aggggtgttt tggttggtat tcatgcagga aagtcttcgt tttagtttga caacagccat
     1681 cgctgttcta gtcattgctt gcccttgtgc gcttggttta gcaacgccga cagcgattat
     1741 ggttggaaca gggcgtgcag ctgaaaatgg tattcttttt aaaggtggtg acatcttaga
     1801 acaagcccat caggtagata cggttgtttt tgataaaacg gggacgatta cggaggggaa
     1861 accaagtctt caaaagctcc taactttttc gggagatgat caccttatct tgcaagaagc
     1921 tgcttccttg gaggcctatt ctcaacaccc cttaggggag gctattgttc gtgctgctca
     1981 ggaagctggt tacgatagtc taccagtgga agagtttgag tctttaaccg gcctaggagt
     2041 tactggtcag cttgaagggc gtcgtttggc gattggaaat gctgctttaa tgggaattct
     2101 tggtatcgat ttatcttgtg ttcagtcagt ttgtgctaag gcttctgaga aaggccaaac
     2161 ccttgtatat tatgctaaag aggggcagct acgagctctt ttcagtattg ctgacgctgt
     2221 caaagaagat agtcaagcta ccgtcgaagc tttgcatcaa ctgggaattc acaccatcat
     2281 gctaacaggt gaccacgatg ctaccgctaa ggctattgcc tctcaagtgg ggattacaga
     2341 tgtgatcagt caagtcttac ctgatcaaaa agcaggcgtg atagcagacc tccgctctca
     2401 agggagaaaa gttgcaatgg taggtgatgg tattaatgat gctccggcat tggctgtagc
     2461 agacattggg atagctatgg ggtcagggac agatattgcc attgaatcgg cggatgtgat
     2521 tctgatgaaa cctgatatgc tggatttggt gaaagctatg tcattgagtc gagcgactat
     2581 gaggattgtc aaggaaaatt tattttgggc attcatttac aatgtcctca tgattccagt
     2641 ggccatgggc ttacttcatc tttttggagg cccattattg aacccgatgt tagcaggttt
     2701 tgccatgagt tttagttcag tatcagttgt tttgaatgcc ctgcgattaa aaggaaaaac
     2761 catttaaagg agaatacaaa atggaaaaac attatcaggt tacaggaatg acatgtgacg
     2821 gttgcgcaag aacggtcact gaaaaattat cagctgtccc cggtgtgcaa tcggtccagg
     2881 taaatttgga aaaaggacag gctaaggtga caggacgtcc tttaacgttc ctactcaagc
     2941 gtgctttgaa ggataccaaa tttgaattac atcaatcatc ttaggttatt ttggtagcct
     3001 tacttcatct caaaagaggt tccagttttt taatctgggc ttcttttttt gtggtcactc
     3061 ataaagactc tgacgttagc aggtgtgttc cgagcaaaat aaaaacacct ctctggaagc
     3121 aaatcacttt tgccagaagg tgtttaagga cgatgcgctg acaggctgtc cattcttgat
     3181 gaaacggtta ctccttgttt tcgtgggcta ctaataattg tgtgaactga ctataacgtt
     3241 cgatgacgtc ctcagttatt tgcgaatctg taacaacagc atcaatgttg tttaaatggt
     3301 aaaaaatgaa gaagtcgtag gcattgaatt tttggttatc taccaagagg tatttttcaa
     3361 tagaattatt aaaagctagc ttttgaatct ctccttcagt ttcgctataa gttgcaatat
     3421 cgtttttata aataccatta cagctaataa aagccttggc aaattttaag gatgagatat
     3481 tttgactggc aagggaccct acaaaagctc ctgtaataga tcggtattca ccgccgataa
     3541 gaatcaggtc aatggtttcg ctgtcctgaa ggatgttaaa gacagggagg ctattggtca
     3601 caatgcgaat ctgacgattt tttaattgct cagcgaaaca ttctaaagtt gttcctggac
     3661 caataaagat tgtttcacca tcattgacta gttgtgtcgc atatgaagct agttcccatt
     3721 tttcctttgt ttgaacggtt tgtttttcaa tatttgaccg ctctttttta ttaggcatgg
     3781 taatgctttg tgcacctcca tggatgcgga tcaatttgcc ggctttttcc agttcatcta
     3841 agtctctacg aactgtcata tcagaaacgt ttaaagtctg aataatatca ttcacagtaa
     3901 caataccttg ctcattgact atttcggtaa tttttagaag tctttctctt tttaacatct
     3961 aaggggctcc tttctatagt ttatttactt atgtttatca tagcatatag accttcatat
     4021 ttcaataaaa caaaataaac aaaacaaaaa caaacatata gaacaaaaaa tattgcgtaa
     4081 aaatgttgaa ttatgttgaa ggttatacta taatgatttt gtaaacggtt accaatttgt
     4141 tgatgatgag atgacagttg ttaaaaattg atacttatag aaaggcggta gggagtgttt
     4201 cctaatattc tatttactga agcaaggacg cagccagagc tttttgatct tgttgctagc
     4261 tacttggaaa aggttgggta tgtgacgcaa gagtatcatc aggctttagt ggagcgtgag
     4321 gcggtatttc cgacaggact caaagtggac ctcaaagatg ggtcagatat tttgtatgcg
     4381 gctattccgc atacagaaac caagtattgc ttggttgatc aagtggtcta tgttagaaat
     4441 agtcaagccc ttacctttaa gcacatgatt aatccagagg aagactgttt ggtaacggat
     4501 ttcttcttta ttatcaatag tcaaaacgag ggtcaaacga cgattctatc caatctcatt
     4561 accttcttta ttacgaaagg caatcttagc tatctagcca gtctaaaaga tgataaacaa
     4621 gctatttcaa actatttaat agaaaaagga gtgtttcagc atgattaaaa ttctagcagc
     4681 ctgcggtgca ggggttaact caagccatca aattaaagat gcgattgaaa ctcagatgag
     4741 tgatcgtggc tatgatgtgc attgtgatgc ggtgatggtc aaagacatca cagaagaatt
     4801 ggtgtctcgt tatgatattt ttacgccgat tgcaaaaact gacttgggat ttgagatgcc
     4861 aattcccaca gtagaagcag gaccgattct ctatcgtatc ccaatcatga gtgagcctgt
     4921 ttttgctgaa ttagagcgcg tgattaaaga aaaccatttg cagtgagtta ataaaaacat
     4981 aaggagacaa gatgatgcaa ccttttttag acattattaa caagattctg ggatttccta
     5041 ttcagttggg atcaggtgtg gtcatgctga ttgtcatgac aggtttagcc atgattttcg
     5101 gggttaaatt cacaaaggca ctggaaggcg ggattaagtt agctatcgct cttacaggta
     5161 ttggagccat tattgggatt ttgacggggg ctttttcaga gtctttgcag gcctttgtta
     5221 aaaatacagg gatcagttta aacattattg atgtcggttg ggcaccgctt gcaacaatta
     5281 cctggggttc cccttatacc ctttatttct tgctggtgat gctggtggtt aatattgtca
     5341 tgattgtcat gaaaaaaacg gacacccttg acgtcgatat ttttgatatt tggcatttgt
     5401 ctatcacagg tctattgatt atgtggtatg ctgatcacaa ccatttgcca gtccttgtgt
     5461 ctcttttgat tgctactgtg gcagttattc ttgttggggt attgaaaatc attaactctg
     5521 atttgatgaa accgaccttt gatgaccttc ttgggacagg gtctcaatca ccaatgactt
     5581 caactcacat gaactacatg atgaatccaa tcattatggt cttagataag atttttgaca
     5641 aggtctttcc ggggctagat aaatatgact ttgatgctgc taaattgaac aaaaaaattg
     5701 gcttctgggg ctctaagttc tttatcggaa tggctctagg ttttgtgatt gggattatgg
     5761 gagatcctca tttcactgtt gaatctatca aaaattggtt tggtctagga tttacagctg
     5821 gtgcctgctt ggaacttttc tctttgattg gctcttggtt tatcgcagct gttgagcctt
     5881 tgtcacaagg gattaccaat tttgccaatg ctagaatgca tgggcgtcgt ttcaatatcg
     5941 gtttggactg gcctttcatt gctggtcgcg ctgaaatctg ggcctgtgcc aatattcttg
     6001 ccccaatcat gttgattgaa gccgttctcc tatcaaaagt tggcaatggg attttaccat
     6061 tggcggggat tattgctatg gggatgacac cagctcttct tgtggtcaca cgtggtcgtt
     6121 tgattcgcat gattattttt ggttccctct tattgccatt attcctcttg tcaggaacta
     6181 tgattgctcc atttgcgaca gagttggcta aaaaagtagg ggcttttcca gcaggtacaa
     6241 gcgctggttc cttgattacg cattcaacac ttgaaggacc aatggaaaaa atttttggtt
     6301 atgtgattgg tcaagcgaca acaggtcaga ttgcctcaat catcacgcta atcatctttg
     6361 tggccattta cctaggtctc tttgcttggt atgctaacca aatgaaggca cgtaacgctg
     6421 agtatgcgaa aacaatgaaa taaaatcagg tcaggtgact gactaaaaaa ttagggagaa
     6481 tagcagataa ctgcgtaagg tatttctgcc ttacccccta acgtgaaaag agtatttctc
     6541 atgtaaggca aggcgctctg tgggaactca caagtgcttt gtcttgaaag aaataatgga
     6601 agacactaag actactggca atcatcttga tctaaggaca ggtaatcatg tcccccttct
     6661 ctagattggc aagcgttttg ggttagaaaa ggaaaagcta tggctattat tttaggtgca
     6721 gatgcacacg gaaatgcatt aaaagaattg atcaaaagtt ttcttcaaga agaaggctat
     6781 gatattattg acgtgactga tattaacagt gattttattg ataacacatt agcggtggcg
     6841 aaagccgtta atgaagcaga aggtcgttta ggcattatgg tagatgctta cggagcaggt
     6901 ccctttatgg tagccacaaa attaaaagga atggttgctg ctgaagtgtc agatgaacgc
     6961 tctgcttaca tgacgcgcgg tcacaacaat gcccgcatga tcacaatagg ggcagagatt
     7021 gtaggtccag aattagcgaa gaatatcgtc aaaggttttg tgactggtcc ctacgatggt
     7081 ggtcgtcatc aaattcgtgt tgacatgctc aataaaatgg cctaaaggag gataaagtga
     7141 tgaaaattgc gattggttgt gaccatattg tcaccaacga aaaaatggct gtttctgatt
     7201 ttttgaaatc taaaggctat gacgttattg actgtggcac ttatgaccat acaagaaccc
     7261 attaccctat ttttggaaaa aaagtcggcg aagctgtggt taatggtcaa gcagacttgg
     7321 gtgtttgtat ttgtggaact ggagttggca ttaataatgc cgtgaacaag gtaccaggta
     7381 ttcgttcagc ccttgttcga gatatgacaa cagcgcttta tgccaaggaa gaattgaatg
     7441 ccaatgtgat tggctttggt ggtaaaatca ctggtgaact tcttatgtgt gatattatag
     7501 atgcttttat caaggcagaa tacaaagaaa ctgaagagaa taagaagcta attgctaaaa
     7561 tagcccactt ggaaagtcat catgccaacc aagaagatcc tgattttttt acagaatttt
     7621 tggaaaaatg ggatcgtggt gagtatcacg attaggaaga agagatgagt ttaacagtta
     7681 tcttaaatcc ttctgtcgct atttcctatc ctttagattg tctggctatg gacacggtta
     7741 atcgtgttga cagaacggca aaaacagctg gtgataagga gctaaatgtg accagaatgt
     7801 tagcaaaagc tggtcagtta tggcaacagg atttagtggt ggtaaactgg gtgactttat
     7861 cattcatcag ttacaagaac agggcatttc taaccaattt ttcaaaatca aaggggaaac
     7921 gagaaattgt attgctgttt tacatgaagg catgcagaca aaaattttgg aagctggtcc
     7981 ctacagtgat gtggacgaag ccgaagtctc tttgagccac atgagtacta ttgctaagcc
     8041 gtttgatgtc ttaacttctc aggcagtctg cctaggagac tagctactca ctattatcaa
     8101 gatttgatga ctatggcaac ttttaaacaa ttatatcagc aacttcaagt aagagaggta
     8161 tcacatgaca atcacagcaa ataaaagaca ttacttagaa aaagtgagcc atcaaggaat
     8221 catatcagct ttagcttttg atcaacgagg tgcactgaag caaatgatgg cagcacatca
     8281 agaaggagaa gcgacagtta cacagataga aacattgaaa gttctcgttt ctgaggaatt
     8341 gacgccttat gcttcatcta ttttattaga tccagaatat gggttgctgg ctactaaggt
     8401 aagagctaat cagacaggct tactgttagc ttacgaaaaa acaggttatg atgctacaac
     8461 cactagccgt ttacccgatt gcttagtgga atggtcggtc aaacggttga aagcagcagg
     8521 tgcagatgct attaaatttt tgctctatta tgatgttgat ggagatgaac aaatcaattt
     8581 acaaaaacaa gcttatattg aacgcattgg ttcagaatgt acagctgaag acatcccttt
     8641 ttttcttgaa cttttgagtt atgatgagag aatatcagat aacaacagtg cggcatacgc
     8701 aaagcttaag ccacataagg ttaacggtgc tatgtcagta ttttcggata agcgttttgg
     8761 cgttgatgtg ctaaaagtag aggttcctgt taatatggct tatgtagaag gttttactga
     8821 aggggaagtc cattacagtc aagctgaggc tattaaagct tttcaagacc aagaagctgc
     8881 cagccacctc ccttatatct atttgagtgc aggcgtatct gccaaacttt ttcaagagac
     8941 attatacttt gcagcagcag caggtgctca atttagcggt gttttgtgtg ggcgtgccac
     9001 gtgggcagga tccgttccag tttatattac taaaggagag gacgaagcaa gaaagtggct
     9061 atgcactgaa ggcttccaaa atattgacga gttaaacagg gtactcgaag agacagctag
     9121 cccttggacg gaaaaaatct aaaacaacct tatcctatca agcagctctc attgatgtga
     9181 taagataagt atggggataa ttgtttgaat cttttgtagc aaggatgaaa atgggtttta
     9241 tccttgcttt tttatcctta cattttatct ttttctactt attttttaaa ggaaaacgtg
     9301 gtaaactaaa agaagactaa aagacaatag ggtagcttat gacaattgat atgtgggaaa
     9361 ttttaaatat catcgggacc attgcctttg ctctttctgg tgctattgtt gccatggaag
     9421 aagaatttga tattttaggg atttttattt taggtttcgt gacagccttt ggaggcggag
     9481 ccattcgaaa caccctaatt ggtttaccta tagaggcctt atgggggcaa aagccagaat
     9541 tcacttgcgc tttctttgct atggttttga ttatgctttt tcctaagcta atagctagag
     9601 gttgggtacg ggcagttgtt ttgactgatg ccattggtct ggcagctttt agtgtccaag
     9661 gagccttgca tgctgtccgt cttaaccagc ctttaagtgc tgtcattgta gcagctgtgt
     9721 tgactggtgc aggcggtggt gttgtcagag atattctggc agggcgaaaa cctagtgttt
     9781 tgcgtagtga aatctatgct gggtggtcta ttcttgcagc tattgttttg cattttaaac
     9841 tagcagattc aacgattgaa tgttatgcct tggttgtttt actcacgact ttacgcatga
     9901 ttggcaaccg taaaaaatgg aacctcccta aaatcaaatg gaaagaggaa agataggatg
     9961 attcaactaa ttgcaattga tttggacggc accttattaa accaggacaa acagattcca
    10021 aaagagaata tcacagccat ccaagaagca gctcagtcag gcttaaaaat tgtcttatgc
    10081 acaggccggc ctcagtcagg gacaagaccc tattttgacc aattaggttt gacacaagag
    10141 gaattcctca ttatcaataa tggctgtagt acttatagta gtcctgactg gcaattacgc
    10201 cattcaaaaa tgttgaaggt ttcagatatt gaattgctgg aagagctaag ccaaagtttt
    10261 ccagatattt acctgacttt aactgaggaa aacgattatt tggtgcttga agaagaagtg
    10321 ccagaccttg tgcaggaaga tggagatttg gtatttacca ttgtcaagcc tgtctcctta
    10381 gcagagctgt cggacactcc tcgcctgatt tttcaagcca tgtacttagg agaaaaagct
    10441 gcgctagatg cctttgaacg tgcggtgaga aatcagctta gtcagagttt tcatgttgtt
    10501 cgtagccaag ataatatttt agaaattctc cctcagggag tcagcaaggc atcggctctc
    10561 aaagaattag tagaagattt gggtttaact gctgaccaag tgatggctat tggggatgct
    10621 cccaatgaca ttgagatgtt aacttatgct ggtttaggag ttgctatgga aaatgcttca
    10681 gcagctatca agccattagc tgacaaagtc actttgacta atgatatggc gggtgtggcc
    10741 caggctatcc gtcagtttgc acttgcagct caaaaagact aactattata taaaaaaggt
    10801 tcccaaaagg aaccttttta cgttaataga ggcaagccca tagcagccat ttcttcggca
    10861 ggaacgatca tgtcatctga aatccagttt gtcaagacag aaacaatagc attgctccaa
    10921 aaaaggcgct tatagtgcga ttgttttttg tcttttaatt tttgataagc tttcagacgt
    10981 ttagtgacta atcgtgttag aagatggtgc aggtgatgtt tgaaaatcat tttaagaagc
    11041 tgagcttctt ttttagcttc agtaaaaaga tagagccagg cttgataagc ctgagtttta
    11101 atatcaaatt gttttagtcc acgaaaaata cgacggataa cccctactaa cagttgttct
    11161 ataatggctt ctttagaggt ataattacga taaaaagcgt tacgagatac tcctgctttc
    11221 gtcaccaatt ctgaaatcgt aatatcacct aaaggttttg tttctagaag attcagtaag
    11281 gcgatttcca tagattcacg tgctatgcgt cgtgtttctt tattaaattg tgttaagttt
    11341 tgaagtgatc tttttgagag tgctttatca gtcatcacaa atacctttca agtgttacct
    11401 ctgatgagtt atcagtagca aaaaccgact taataatgat ataattttat gggaaaagtc
    11461 ttagtttgtc aactaaagtt agttatcagg agcctcatta cctccttgta tagctctatt
    11521 ggatgacttt actaactatt gaaaaggcta gtaataaaac ataggattta atgattagtt
    11581 attaccatta gcctagacat cttagcgaag gcttaataat ctggtctttg ttaccgaaca
    11641 caaattagag aaaaagaagg ataaaaaatg acttggaaaa ttgtaacaga ttcaggatgt
    11701 gatcttagaa gtttgactag acaatccaaa gagttgcgct ttgaacgtgt gcctttgacc
    11761 ctacagattg gcacagaaat ttttagagat gatgatggat tagatatcga taatatgatg
    11821 acaactatgt accagtcgtc taaagcaacg acatctagct gtccaagccc agaagccttt
    11881 ttgcaggcct atagaggtgc agataatgtt atcgtgatga caattacagg gaccttatct
    11941 ggcagtcata atagtgcacg actggctaaa aatgaacttc ttgaagaaaa tcctaatgtc
    12001 aatattcacc taatcgattc tttatcagca ggtggcgaga tggatttgct cgtactggag
    12061 cttgagcggt tgatcaatct tggattatcc tttgaagaag ttgtcaaaca gatcacagct
    12121 tatcagcaaa aaacacgttt gatttttgtg cttgccaagg ttgataattt ggttaaaaat
    12181 ggtcgactga gtaaattagt aggtaaagta attggcttac tcaatattag aatggttggg
    12241 aaagctagca ataaaggaac gcttgaactt cttcaaaaag cgcgtggtca aaaaaaagca
    12301 gtttctgctt tgatagaaga aattcaaaaa gagggttatg taggcggaaa agtttacatt
    12361 gctcatgctc aaaatccaaa aatttgtgag cagatcagtg aaaaaatcaa atctttatat
    12421 cctgatgctg tgattcaaac tggaaggacc tcaggtttgt gtagctttta tgctgaagac
    12481 ggaggacttt taatgggcta cgaaatttaa tgctatctaa caaaacaaaa agctagactc
    12541 ttttgatttg agctagcttt tttaagatat caggaagact aatcactaag atagccgtct
    12601 aaaacgttta ttgattgatt ttacgaaaaa tttttgtatt actcttttga tgattgcttg
    12661 tctcctgagt cagaaggttc tgaagaggtc tgaggtggaa ttggatcagg atgactaggt
    12721 tgtggcgtaa catctgaagc tgaagattct gagcttgggt ctaaagtagg tgactgggaa
    12781 ttatcataag tatctgagac gtctcctggg aaaggtgtcc agtaagagtt atctgttatt
    12841 tctccacttt gtcttggaaa ttcatcaata atgggttttg ctggtaaaat agcgtctaac
    12901 cgagattgta aagcggcttt tttacctttg ttacttaagt cgttaactgc tttttgggcg
    12961 atatctttaa gattttgatt gagagtctcc tcagcagctt taactgcttg agtagctgca
    13021 tcttcttgct cttgctttgc tttggcaacg tttagctgtt cttgtagcgt ttttttctta
    13081 gaagattctg ctaatttatc aacagctttc tgggcttctt ggatagcagc tgcagtggct
    13141 aaacgatcag cgtcattaac tgctttaact gcttttttta gtaaactagc agcactttta
    13201 gaatgtttag ctaccttatc ttgcgtttga gggtgggatc caaaaataag gcaggagcca
    13261 cctattagag tgatagttag aacactcaac atccaataaa gcgcctcttt ttgtgattta
    13321 gtcatggaat ctcctttatg atattgtttc atcgtagcca tattatacct ctaattaaac
    13381 gaaatgaaaa gatttgtgca aaaacaaatg atgtaaaata tcggaaaagc ctaacacctt
    13441 atcaaaccaa taatatgcaa atgttgacat cggtatacac agaaacgctt aaaaaaacct
    13501 tgtgataaag accgtaatac attaaaatag aatagactaa aaaattgagc gaggacaata
    13561 ttgtgtcagt aaattggcaa gacattgcat ttcatttctt tggaggctta ggtctctttc
    13621 ttttcagtat caagtatatg ggagatggtc tgcaacaagc agcaggagat aaactaaggt
    13681 attacattga taaatacacc agtaatccct tttttggtat cttagtcgga attgccatgt
    13741 ctgccttaat tcagtccagt tcaggcgtaa ctgtgattac ggttggtttg gtttctgcgg
    13801 gtttgctaaa tttgcgacaa gccattggaa ttgttatggg ggctaatatt ggaacgacta
    13861 tcacttcgtt tttaattggt tttaaactag gagattacgc cttgcccatg atttttattg
    13921 gtgcagcctg tcttttcttt acctctaata aaaagctcaa caattttggg cgtatcattt
    13981 ttggtgtcgg aggaattttc ttctcgctga acctaatggg agatgccatg gatcctttga
    14041 aatctgtgtc agcctttcaa aattatttag cgacattagg tgataaactc ttccaaggtg
    14101 tctttatcgg aacggctttg accatgttga ttcagtcatc ggcagccatt atcgggattt
    14161 tacagggtct gttttcaggt ggtcttttga ctttacaggg agctattcct attttgttag
    14221 gctcaaatat aggaacctgc attacagctg tcttagcagc tataggttca aatattgctg
    14281 ctaaacgtgt ggcagcagct catgtgttat ttaatttgat cggtacaatt atttttatga
    14341 ttatcctagt cccattcaca tcattgatgt tatggctaca atcaaagttg agtttaacgc
    14401 ctgagatgac tatcgctttt tctcacggtt cttttaatat cactaatacc atcttattaa
    14461 ttcctttcat tagtctacta gctatgattg tgactagact cattccagga gaagatgaag
    14521 tggtcaaata cgaagccctt tatttagatc gcttacttat tacccaagcc ccttctatcg
    14581 cactaggaaa tgcccacaag gaattggttc atttagcctc ttatgcgatt caagcttttg
    14641 aagcttctta cagttatatt atgacagctg acggtaaatt tggggaaaag gtcaagcgtt
    14701 atgaaagagc agttgatacc atcgatgagg agttaaccac ttatttggtt gatatttcta
    14761 acgaagccct cagcccaagt gaaaatgaag tcttggctgg tattcttgac tcttcacgtg
    14821 atttggaaag aattggagat cattctgagt ctttaggcat tctaattgaa ggcatcatat
    14881 ctaaacaaat tggtttttca atatctgctc gtcaagaatt gacagagatg tatcaattga
    14941 cgcattgttt aacactggat gccattagag ctatcgttga tagtgataca gacttggcgc
    15001 aaactattgt gactcgccat aaagaaattg aagaaaaaga gcgacgatta cgtaaaaccc
    15061 acattaagcg cttgaattgt ggagaatgta cggcacaagc aggtattaat tttattgata
    15121 ttatttccca ctatacccgc atcacagatc atgccttgaa tttagcagag aaagtacttt
    15181 cacaccagct ttaaaaaaga gaaaacgtga atgatcatcc acgttttctt ttttgaatcc
    15241 aaaaagtggt atataccatt tacaaagtaa agaataaggc gtataataag gaaaaaaggg
    15301 aggaaaaaag atgacatgct atcttaaagc agattgtttt tattacccaa cagaggtgaa
    15361 accggctggc tacctaggtc ttcatgatgg tgtttttggg gagtggacag aaattgttcc
    15421 ggcagatgcg caaatcattg actacaccgg ttatcaaatt gcaccaggtc ttgtggatac
    15481 gcatatccat ggctatgcgg gagctgatgt catggataat tctgcccaag ggattcatca
    15541 aatgagtgaa ggactattag caacaggagt aacgtctttt ttaccgacga ccttaacttc
    15601 tacctttgag cagttagaaa aagtatcagg gactattgct tcagttgctg atcaggtaaa
    15661 aggtgctaaa atacaaggta tctactttga aggtccttat tttaccgaag aatataaggg
    15721 tgctcaaaac ccaagttata tgaaaacccc acgcttggag gaatttgatg cttggcaaaa
    15781 agctgctaaa gggctcatca aaaaaattgc ccttgctcct gagcgtgagg gtgtcaaaga
    15841 atttgtgtct gcagtgacaa aacaaggcgt aacagtggcc ttagggcatt caaatggtac
    15901 ttatcaagag gctaaagaag ctgttcaagc tggtgctagt gtttgggttc atgcttataa
    15961 tggcatgcga ggattgaccc accgtgaacc aggaatggtg ggagcggttt ataatcttcc
    16021 aaatacttac gcagagttga tttgtgatgg ccaccatgtc tcaccaattg cttgtgatat
    16081 tttgatgcag caaaaagggc atgatcatgt agccatgatt acggactgta tgcgagcagg
    16141 tggttcacca gatggtgatt acctcttggg ggaattctct gtggttgtgg caaatggcac
    16201 tgctcgtctt aaagagagtg gaaacttggc aggatctatt ttgaaattaa aagacggcct
    16261 tagaaatgta gtggcctggg gaatcgcaac tccggcagaa gccattcata tggcaactta
    16321 tgtgccagct gtttctgtcg gtattgacga cgtatgtggt caaatcaaag ctgggcatgc
    16381 ggctgacttt attgttcttg ataaggattt aacacttgtt gcaacctatt taaatgggca
    16441 aaaagctttt gatgcctaag agatacaaaa ggattggagt agctatgtct ggcaggccaa
    16501 ttctttttgt attttctttc tttatgatag aatagattaa agtaatcgct tacagatatt
    16561 gataaaagga ggtttttgtg atggtaacaa cagtaaaaat gacaagtggc tatgagattc
    16621 ccgttttagg atttggaact taccaagcag ccgatggtga ggaagcttac caatctacct
    16681 tagcagccat caaggctggc tatcgtcata ttgatactgc agctatttat aaaaatgaag
    16741 aaagtgtggg tcgtgccatc aaggattctg gggttctacg agaagactta tttattacaa
    16801 ctaaactctg gaatgacgcc catagttatg aaggggcaaa agacgcttta gctgcttctc
    16861 ttgaccgtct tggtctggat tatgtggatc tctatcttat ccattggcca aacccaaaag
    16921 ctctgcgtaa cacttggaaa gaggcaaatg cccaagcatg gcaatatatg gaagaggcgg
    16981 ttgaagcagg gcttattaaa agcattggcg tcagcaattt tatggtgcat catctagaag
    17041 ctcttcaaga gacagctaaa ataactccag caattaatca gattcgatta gccccaggtt
    17101 gctatcaaaa ggaagtggtt gattattgta aggcaaatga gattttattg gaagcttgga
    17161 gtcctctagg acaaggagag atttttgata atgagactat gcagcagtta gctaataaat
    17221 atgacaaaac agtggcacaa gttgccttag cttggtcgct ggcagaaggt tttattccct
    17281 tgccaaaatc ggtgcatgat gagcgcatca aggaaaatat ggctattttt gatgtgagct
    17341 tgacacaaga agataaaaaa acaattcgct atctctcagg aatgtcagcg attcctaacc
    17401 ctgatactac aagtttttaa ggaactacaa agatttagcc ataattcgct aaatcttttt
    17461 tgaaaacggt ttgacaaagg tttatcattt agctataatc tttattagtg ttttgtgaaa
    17521 gaaactatta ttgaaaataa ggagccgtat tgtgaccatc tttttagtga tgttagctat
    17581 gtttactatt cgtttagtat ttttgaagaa atcgataaca aatgaaaaag ccatattagc
    17641 ccaaggagga caggaatttg gtgctcagaa cacgaaattt ttgacgttac ttcacattat
    17701 gatttatgtg tttgcggtga ttgaagcgct gcttaaacaa attaagtttg atggcattag
    17761 ttttttaggt cttttgttga tgttattatc agtagctgtt ttatatgaag tgacacgtat
    17821 cttaggtgac atttggactg taaaattgat gttagcaaaa gaccacaaat atgtggatca
    17881 ctggttgttc aaaacgatta aacatcctaa ctacttttta aatatcgccc ctgagctggt
    17941 gggcatagcc ttactttgcc atgctaaaat tacggctatg cttcttttcc catgctacat
    18001 tgtagtaata tatttacgta ttcgagaaga aaacaagcta ttagcggaag tgattattcc
    18061 aaacggtact cgaacaaaac cttaagctca tgatggacaa actaatggct ttatgctaaa
    18121 ctagactcta ggaaaaagga tgcaagtatc ttatctaaag cgagttcggg gtagttagag
    18181 ccgaatggta ggactgcaga ttggcgcttc cgtttgggca gtgtgattaa gtatatttgt
    18241 caatattgcc caaaaagata ctatataaat gaagtaataa attagggtgg aaccgcgttt
    18301 tgacgcccct aggtcttatg atttaggagc atcaaaatgc ggttttttgg tgcctagacc
    18361 tgtaatacat gctagtaaca tcgtcaggta ttttactgat gacaaaaaaa cgattgtcaa
    18421 tgatatacca ttttactact atcaaaggag tacgaaatgt ctaaaaaact tacctttcaa
    18481 gaaatcatct tgaccttaca acaatattgg aatgatcagg gttgtatgct catgcaagcc
    18541 tatgacaatg aaaaaggagc tgggaccatg agtccttaca cgttcttacg tgctataggt
    18601 cctgagccat ggaatgcggc ttatgtagag ccatcacgtc gtcctgcaga tggccgttac
    18661 ggagaaaacc caaatcgtct ttaccaacac caccaatttc aagtggtcat gaaaccatca
    18721 ccatcaaata ttcaagaact ttaccttgct tctttggaaa aattgggcat caatccttta
    18781 gaacatgata ttcgttttgt tgaagataac tgggaaaacc catcaacagg atcagcaggg
    18841 cttggttggg aagtttggct tgatggaatg gaaatcacac aatttacgta tttccaacaa
    18901 gttggtggcc ttgcgacatc tccagtgaca gcagaagtaa cttatggtct tgagcgctta
    18961 gcttcctata ttcaagaagt ggattcggtc tatgatattg agtgggcacc aggtgttaaa
    19021 tatggtgaaa tcttcttaca acctgagtat gagcattcaa aatattcctt cgaaatatca
    19081 gaccaagaca tgctgcttga aaactttgaa aagtttgaaa aagaagcctc gcgtgcccta
    19141 gaagaaggtt tggttcaccc agcttatgat tacgtgctca aatgttctca tacgtttaac
    19201 ctgctggatg cccgtggcgc tgtttccgtc acagaacgcg caggctacat tgctcgtatc
    19261 cgtaatttgg cacgtgtcgt tgctaagacc tttgttgcag aacgcaagaa gcttggattt
    19321 ccactgttgg atgaagcaac acgcgcaatc ttgctagctg aagatgacga ataagtcgat
    19381 ttaaagatga tctagttgcg agctttgtaa tcttaagagc gagcaagaag tgggcgaccg
    19441 aaggtcgccg agtcaaggtt actcccgctg tagtgacaat tcggtaaggt tacttatcga
    19501 acgagggagt tggcgagact ttaggctggc taactaggaa tggaacactt ttgttttctg
    19561 aaattatcca ctggataatt tcactttgcg tcccccctac ctaagggaac aatcaaaaag
    19621 atacttttta gagaaaggaa ctgaacatgg gctacggact atgtcaaaaa gaccaagact
    19681 tcctaggtgc aagcacctgc gtcgctttgc tattttgatg gtgtccgctt aacgcccttg
    19741 gtatcttaat tatgagtaaa aatctactaa tcgaacttgg tctggaagaa cttccagcct
    19801 acgtggtcac accaagtgaa aaacaactgg gagagcgctt ggcgactttc ttaacagaaa
    19861 atcgtctctc ttttgaagat atacaaacct tttcaactcc acgccgtttg gcagtgcgtg
    19921 tgtctggatt agctgatcag caaacggatt tgactgagga ctttaaaggg cctgctaaga
    19981 aaattgccct tgatgctgat gggaatttct caaaagctgc tcaaggattt gttcgtggca
    20041 aaggcttgac cacagatgct atcgaattcc gcgaagtcaa aggagaagaa tacgtttatg
    20101 tgaccaaaca cgaagctgga aaacctgcta aggaagtgct tctaggcgtt actgaagtgt
    20161 tgtcagcaat gaccttccca gtcagcatgc actgggctaa caatagtttt gaatatattc
    20221 gtcctgttca caccttgaca gttctgttga atgatgaggc cttggaactt gacttcttgg
    20281 atattcattc tggacgcgtc agccgtggtc accgtttttt aggtactgaa accactatca
    20341 caagtgctga ttcttatgag gctgatttgc gcagccaatg cgtcattgtt gacgctaaag
    20401 agcgtcaaga gatgattgtg gagcaaatca agacgcttga agtggagcag ggtgtgcaag
    20461 ttgacattga cgaagacttg cttaatgaag ttttgaactt ggtcgagttt ccaacagctt
    20521 tcatgggaaa ttttgaggct aaataccttg atgtcccaga agaagtattg gtaacatcaa
    20581 tgaaaaacca ccaacgttat tttgtggtac gtgatcaggc tggtcacttg atgccaaact
    20641 ttgtatcagt tcgtaatggg aatgatcaag ccattgaaaa tgttattaaa gggaacgaaa
    20701 aagttttggt agctcgctta gaagatggtg aattcttctg gcgtgaggat cagaaattgc
    20761 aaattgctga tttagttgcc aaattgacca atgtgacctt ccatgaaaaa atcggctctc
    20821 ttgctgaaca catggatcgt acgcgtgtca ttgccgcatc acttgctaag gaagccaacc
    20881 tatctgctga ggaagtcaca gctgttgacc gtgctgccca aatttacaaa tttgaccttt
    20941 taacaggtat ggttggtgaa tttgatgagc tgcaagggat tatgggtgaa aaatatgctc
    21001 ttctagctgg tgaagatgct gctgtggcaa cagcaatccg tgaacactac ttacctgatg
    21061 ctgcaggagg tgcgctacct gaaaccaaag ttggcgctgt cctggccttg gcagataaat
    21121 tggatactct cttgtcattc ttttctgttg gattgattcc atctggctct aatgatcctt
    21181 atgctctacg tcgtgcaacg cagggtatcg ttcgtattct tgatcatttt ggatggcgca
    21241 tcccaatgga taagctggta gatagcttat atgacttgtc ctttgatagt ttgacctata
    21301 ccaataaggc ggatgtgatg aactttatcc gtgctcgtgt ggataagatg atgggcaaag
    21361 ccgcaccaaa agacattcgg gaagctattc tagcaagttc aacctttgtg gttccagaaa
    21421 tgttagcggt agctgaagct cttgtcaaag ccagccatac ggaaaattac aaaccagcag
    21481 ttgaatcttt gtcacgtgct tttaatttgg cagaaaaagc ggacgcttct gttcaggtgg
    21541 accctagcct ctttgaaaat gagcaagaaa acactttgtt tgcagctatt caaggcttaa
    21601 ctctagcagg cagtgctgcc cagcagttag aacaggtctt tgcccttagc ccagtgatta
    21661 atgatttctt tgataataca atggttatgg cagaagatca agcccttaag aacaaccgtg
    21721 tagctatttt atcagattta gtaagcaaag ctaagaccat tgctgctttt aaccaactaa
    21781 acaccaagta aatagaaagg aaagagccgt tggggactta gctagaaaag tgagttcagt
    21841 acccgagtgg aaagaacaag gcctttaaac attgcttttc acagtgagag tcaagtcagt
    21901 tttttaagtc ttacactttt ttcccaggct cttagtggct taacatggat cctaaaaaaa
    21961 ttgctcgtat caatgagctg gctaaaaaga aaaaaacggt aggcttgact ggacctgaaa
    22021 aagtggaaca ggcaaaactc cgtgaagaat acattgaagg ctatcgtcgc tctgtcagac
    22081 accatatcga aggcatcaaa ttggtggacg aagaaggaaa cgacgtgacc cctgaaaaat
    22141 taagacaggt gcaacgtgaa aaaggcttgc acggccgttc attagatgac ccaaaatcat
    22201 aagacaaaaa gcagctctga actgcttttt gtttatcgtg atgactgtta gcttttattt
    22261 taaatggtat gatacttcaa agtgaccaca cgtttttata ttgtatagtc aaaatattgg
    22321 gaaaaaccat gtgtttgagt tataatagaa gaaatcaaag aaagaaggta gtacttatga
    22381 caattaaaaa attctctcta ctagccatag ccagcctctc tttactcagt cttgcagcct
    22441 gtgatatgga tgataaagac gatcatatgg ataatcaacc aaagacaagt caaactagca
    22501 agaaagttaa actttcagaa gacaaagcca aatcaattgc gttaaaagat gcttctgtta
    22561 cggaagctga tgctcaaatg ctatcagtta cacaagataa tgaagatggc aaagccgtgt
    22621 atgaaatcga gtttcaaaac aaagatcaag aatacagcta taccattgac gccaactcag
    22681 gggatattgt ggaaaaatct tctgaaccaa tcaatgacta atcaagaact gtaatgaata
    22741 agacagcctt aactcttgtg tccaattgtc tttgcctctt aggcgcggct gtttgatacc
    22801 gtgttgcctt tgaggcttag ttctctttat gacccaatga tgactctgga ttagcagagt
    22861 gattgttggg tcttttgatt ttctttgata agaaagatta cctcgttaga aatgaggaga
    22921 cgtggttgtc atcagtggat ttttcaagat catctagtgg gataagctac ctatcttatc
    22981 agcttttttt tagctttaaa aattcttgat aatatatttc tgataaaatt agctataata
    23041 gaatcaaata ggaaaacgct tacttaaagg agggtaatga atgtcgcaag aaaagtatat
    23101 catggctatt gatcagggaa caacaagttc acgagcgatt attttcaatc aaaaagggga
    23161 aaaggttagc agtagccaaa aagaattccc tcagattttc ccacacgctg gttgggtaga
    23221 gcacaatgct aatcagattt ggaattcggt tcagtctgtt attgcgggtg cttttattga
    23281 gtccagcatc aaaccaagcc aaattgaagc aatcggaatt accaaccaac gtgagaccac
    23341 agtggtttgg gacaaaaaaa cgggtgtacc tatttataat gctattgtgt ggcaatcacg
    23401 acagacggca ccaattgctg agcaactcaa acaagatggc catacaaaaa tgatccatga
    23461 aaagacaggc cttgttattg atgcctactt ttctgcaacc aagatccgtt ggattttaga
    23521 tcatgttcct ggtgctcaag aacgagctga aaaaggcgag ttactttttg gaaccattga
    23581 tacttggctg gtttggaaat tgacagatgg tgctgttcat gtgacggatt attccaatgc
    23641 ggctcgtacc atgctctata atattaaaga cttaacatgg gatgatgaga ttttagagct
    23701 tttaaatatt ccaaaagata tgcttccaga ggttaaatcc aattctgaaa tttatggcaa
    23761 aacagctgcc tttcattttt atgggggcga agtgcctatt tcaggtatgg cgggtgacca
    23821 acaggcagct ttgtttggcc aactagcttt tgagcctggt atggtgaaaa atacttatgg
    23881 aaccggttct ttcatcatca tgaatactgg tgatgagatg cagctatcga gtaataacct
    23941 tttgaccacc attggttacg ggattaatgg gaaggttcac tatgctttag aaggctctat
    24001 ttttattgca ggctctgcca ttcaatggtt gcgagatggt cttaaaatga ttgagacctc
    24061 accagagtct gaacagctgg cactagcatc taccagtgat gatgaagtct acgttgtgcc
    24121 tgcctttacc ggtttaggag ctccttactg ggattctaat gcgcgtggat ctgtctttgg
    24181 cttgacacgt ggtaccagca aagaagactt tgtcaaagca accttacaat ctatcgccta
    24241 tcaagtgcga gatgttattg ataccatgca ggtggattca ggtattgaca ttcaacagct
    24301 ccgtgtggat ggcggtgcag ccatgaataa tatgctcatg caatttcagg ctgatatttt
    24361 aggaattgat attgcccgtg ctaaaaattt agaaacaaca gctctaggag cagctttctt
    24421 agcaggactt gctgtgggct actgggaaga catggatgct ttgaaagagt taaatgccac
    24481 aggccagctc tttaaggcta gcatgaatga atcgcgcaaa gaaaaattat acaagggttg
    24541 gaagcgcgct gttaaagcaa ctcaagtttt cacccaagaa gaagatgcag atgacgatgc
    24601 aaaataacta gaggaggatg gaatggaatt ttcaagagaa acaagacgct tagctctcca
    24661 aaaaatgcaa gaaagagatt tagacctact gattattggg ggaggtatta cgggtgctgg
    24721 tgtggcactt caggcggcag ctagtggcct agatacgggt ctgattgaga tgcaagattt
    24781 tgcgcaagga acctctagcc gttcaaccaa attggttcac ggggggcttc gttacttgaa
    24841 acaatttgat gtggaggtgg tttcagatac ggtgtcagag cgggctgtgg tgcaacaaat
    24901 tgcaccccac attccaaaac cagaccctat gttattacct gtttatgacg aacctggtag
    24961 tacctttagc atgttccgtt tgaaggtcgc tatggatttg tatgatcttt tagcaggcgt
    25021 gtccaatacg ccagcggcca acaaggtgtt aaccaaagaa gaagtcttaa aacgagaacc
    25081 agacttaaaa caagaaggct tgcttggtgg tggggtttac cttgatttcc gtaataatga
    25141 cgcaaggctt gttattgaaa atatcaaacg agctaatcgt gatggggctc ttattgctag
    25201 tcatgtgaaa gcagaagatt tcttgctaga tgacaatggt aagattattg gtgtgaaggc
    25261 gcgtgatttg ctgtcagatc aagaaatcat tatcaaggct aaattagtca tcaacaccac
    25321 aggtccatgg agtgatgaga ctcgtcaatt ctctcataag ggacaaccga ttcatcaaat
    25381 gcgccctaca aaaggggtgc atctggtagt ggaccgtcaa aaattaccag tgtctcaacc
    25441 tgtctatgtt gacacagggt taaatgatgg tcgtatggtc tttgtcttgc cacgtgaaga
    25501 aaaaacttat tttggaacaa cggacacgga ctacactgga gacttggagc acccacaagt
    25561 tactcaagaa gatgtggatt atttgttagg cgttgtcaat aaccgctttc caaatgccaa
    25621 cgtgaccatt gatgatattg aaagcagttg ggctggtctt cgccccttgt tatcaggtaa
    25681 tagtgcttct gactacaatg gtggcaacag cggtaaagtc agtgatgata gttttgatca
    25741 cttggttgat actgtcaaag cctatattaa ccacgaagat agccgagaag ctgttgaaaa
    25801 agctattaag caggttgaaa ccagcacatc tgaaaaagaa ttggatccgt ctgcagtgtc
    25861 acgaggttca agttttgacc gtgatgagaa tggactcttt accttggcag gtggtaagat
    25921 taccgactat cgcaaaatgg ctgaaggagc attgacaggg attattcaaa tcctcaaaga
    25981 agagtttggc aaatccttca agcttatcaa ttcaaaaacc tatcctgttt caggaggtga
    26041 aatcaatcca gccaatgtag attcagaaat agaagcttat gctcaattag gaacacttag
    26101 tggtctttcg atggatgatg ctaggtattt ggcaaacctt tatggttcta atgcgccaaa
    26161 agtctttgcc ttaactcgtc aattaacagc agctgaaggg ttaagtttag ctgaaacctt
    26221 gtccttacat tatgcgatgg attatgaaat ggctcttaaa ccgacagatt atttcttgag
    26281 aagaacaaat cacctcttat ttatgcgaga tagcctagat gctttgattg acccagtgat
    26341 taatgaaatg gctaaacatt ttgaatggtc tgatcaggaa agagtggcac aagaagacga
    26401 tcttcgtcgc gtgattgcag acaatgattt gagtgcccta aaaggccagc aggagggtta
    26461 atatggatat ctttggtgaa tttttgggta cagccttatt ggttttacta ggaaatggtg
    26521 tggtagcagg ggttgtctta cccaaaacga aaacccatgc ttctggctgg atcgtgattg
    26581 cgactggctg gggaattgca gttgctgtgg cagtttttat cagtggcaaa gttgcccctg
    26641 cccacctcaa tccagctgtt agccttgctt ttgccatgag tggaaccatt gcttggtcaa
    26701 cagctattgc ctatagcctt gcccaactat tgggagctat ggttggttca actctggtat
    26761 tccttcagtt caggccacat tatctggctg ctgagagtca ggctgatatt ttagggacat
    26821 ttgcgacagg tcctgctatt cgagatacca gctcaaacct attgagtgaa atctttggga
    26881 cctttgtctt gatgcttggt attttggcat ttggcttata tgatatgcca gcaggactag
    26941 gaaccctctg tgtaggtacc ttggttattg ggattgggtt atccttagga ggaacgacag
    27001 gttacgccat taacccagcg cgtgatttag gacctcgtct agttcatgct attctaccac
    27061 tcaacaacaa gggagattct gattggtctt atgcttggat acctgttgta ggaccaatta
    27121 ttggagcggc cttagcagtt ttgcttttcc aagtcatgtc ctaaccatca ctaaaggggt
    27181 tggctatcat aggccagctc ctttgctttt tctttatagg attaggtaaa acgctttcat
    27241 atcttaaaaa caactgctat aataatacta ttatgacatt aacgacaaag accatacatg
    27301 ttatcggggc atcctttgct ggtctagcct ttgttgataa atataaggac ctcaatccag
    27361 attcccaaat cattctcatt gataaggaaa gctgtcccaa ttatattcca aatggtatca
    27421 accaattatt cagaggagac attcaagacc tgtctgatgc tatgtgggga agggcctgcc
    27481 tagcagcaca aatagaatct aaccaccggt ttattcaagc agaagtgtta gctatcgaag
    27541 ctccctcaaa caccttgctt ctcaaagaca gtcaaggacg agtttttgaa gaaggttatg
    27601 aaacgcttgt ttgtgccatg ggtgctagcc cccagtctca ttatattgag acgtcacaga
    27661 ccaacaaagt tttggtaacc aaatattatg aagaaagcca agctagttta aaactgattg
    27721 aggctagcca agaggtcttg gttattggtg ccggcttgat tggcttagac cttgcctaca
    27781 gtttgtcctt acaaggcaaa cgggtcaaac tgattgaagc agccgaacgt ccagatttct
    27841 atcagactga cgcagaactg atagcccctg taatggcaga aatgtccact caccatgtca
    27901 cctttattaa taataagcgt gttacggcta tccatgagat agagggaaaa gttgtcgctc
    27961 ataccgagca aggggacacc ttccaaggtg atttggctat tttagccatt aactttaggc
    28021 caaatacaca ccttttgcaa ggacaagtgg cttgtgctct ggataagacc attttggtca
    28081 atgaaaactt acagaccagc caggctaata tctatgccat tggagacatg gtttcattgt
    28141 attttggtat attgggaatg gattactata cacctttgat taaccaagca atgaagacag
    28201 gtcaggctct ggctctgcat ttagctggat atcctattcc tccattacag acggtcaagg
    28261 tattaggaag ttctcacttt ggttattatc gcgcgagtgt tggtgtgaca gaagaagaag
    28321 cagagcttta tatggatacg tgtagctatt tgtaccaaaa tggggattct aaaaacctct
    28381 tttggctgaa attaatagct agaaaaacgg atgggatttt aataggcgct cagctcctat
    28441 ccaaaaccaa tgctcttgtc atcgccaatc aattaggtca ggctctggct ctgaaagtta
    28501 cagacggtga cttagcattt caagattttc tctttttaca aggtcacagt gatttggctt
    28561 atcatcttca tgaagcttgt ctcaaattat ttgaaaagag gctccgccat gaagattgaa
    28621 gacttaatgg ataaagaacg aaaagctcaa tatcgtctct tggtgaccct ttatcatgct
    28681 aaagaaacgt taaggctaaa agacctaatg aggctatcaa acttgtctaa agtgacccta
    28741 ttaaaataca ttgacaacct aaatcatttg tgtcgagaac aaggtttagc ctgtcaatta
    28801 cttttagaaa aagacagtct gagcttaaaa gaaaatggcc agttccattg ggaggatttg
    28861 gtagctctgc ttctcaaaga atcggttgcc tatcagatat tgacctacat gtactgccat
    28921 gaacacttta acatcaccaa cttatccgtt gaactgatgg ttagtgaagc tactctaaac
    28981 agacaactag ctcacctcaa ccagctttta tcagaatttg atctggcgct ctcgcaaggc
    29041 agacaactgg gaagtgagct tcagtggcgt tatttctatt tggaattgtt ccgtcatacc
    29101 ttgaccagac aaggcataga cgccttggtc aatcagttag acgcttcgca tttggctacc
    29161 ttaattgagc ggctgattgg ccagtcatta tcggcagaag ccttagaaca attattgatt
    29221 tggttggcga tttcgcaagc gcgaatgtcc tttcaaaaag gctacaatga tcatttttta
    29281 agagatagtg actttatgac atctaatatt ttctttaagc gcttagaaag catgctttta
    29341 cattacttaa gacgctatgc ccttgaattt gatgcttttg aagctaagag cctctttgtc
    29401 tttttgcatg cttatcctct acttcctatt gccagcatga agtatagttt gggttttgga
    29461 ggtcctattg ctgatcacat ttcagaagcc ttatggcttt taaagaaggc tcatgtgatt
    29521 atccatcaga caaaggagga aatcatttat ggcttgggga tatttttttc caaagcttac
    29581 ttttttaaag gagctatcct tagccaacca accaatagcc aataccttta tcaattagta
    29641 ggcgaagata aaagagcgct tttaagggtt atcataaacc atttagtgct gcaaatgggt
    29701 caggaaacgg atttcagtca gcagttgagt gacgacattt tagcattgtt ggttttttcc
    29761 attgagcgcc accatgagcc tcttttagta gggcttgcct tggggcaaaa taaggtggaa
    29821 gcagccattg cggaattggc cattagacgt catttggggc atcgtaggga ttttcaatta
    29881 atgccctatg atcatcaaaa agtttatgac tgtctgatta cctatcagac tgtctattta
    29941 ccgagacaag atttacccta ttaccggtta aaacagtata gctcccccta tgaactcact
    30001 gccttagaag cttttctcaa agaccttttc cagcaaaaaa atacacgaga agaggaactc
    30061 tcactcagcc cagcagctaa gtctagtttt gcacacaaaa ctgtgtgata cttggtcttt
    30121 tctaaggact gttacacttt tataataata aagtaggata gtctaaaggt agatgatttg
    30181 aaaaagctta tcaaatagag cttaacaaaa tcgaaaattt ttggaggtaa gaaatgaaat
    30241 ttttcctaga tacagctaat gtagccgcta ttaaagcgat taatgaactt ggcgttgtag
    30301 atggtgtcac caccaatcca accattatct cacgtgaagg acgtgatttt gaaacagtta
    30361 tcaaagaaat ctgtgacatc gtcgatggcc caatcagtgc tgaagtgaca gggttaacag
    30421 cagatgctat ggtagaagaa gctagaagca ttgctaagtg gcatgacaat gtcgtcgtta
    30481 aaattccaat gaccacagaa gggttaaaag caacaaacat tctttcaaaa gaagggatta
    30541 aaactaatgt gaccttgatt ttcacagtaa gccaaggttt aatggcaatg aaagcaggcg
    30601 caacttacat tagcccattt atcggtcgtt tggaagatat tggcacagat gcttatcaat
    30661 taatcagcga ccttcgtgaa atcattgact tgtacgattt ccaagcagaa atcatcgcag
    30721 caagtattcg tacaacagcc catgtggaag ctgttgctaa attaggcgca cacattgcga
    30781 cgattccaga ccccttgttt gctaagatga cgcaacatcc cttaacgaca aatggtttga
    30841 aaacctttat ggaagattgg gcttccttta aaaaataagg ctaaagagtc tgtggctact
    30901 gtgtcaacag gctctcttat attcatcgtc caaaagaatt caccaatgat gtccaaacta
    30961 gtttttttct ggcaaaatag agaaaaagaa tttagggact ttggaggatt ttctgaaaaa
    31021 agcgtctatt tttgtgatac aatagacaat agaaaacgtt taattttagt tgtaataaac
    31081 agagaggttt tacttatgac atttgatgca attgaccagt tggcagtaaa cactgtccgc
    31141 accctatcaa tggatgccat tcaagcggca aattctggac acccaggact tccaatgggg
    31201 gcagcaccta tggcctatgt tctttggaat cacttcatga acatcaatcc caaaacaagc
    31261 cgtaattggt caaacagaga ccgttttatc ctatcagcag gtcatggaag tgccatgctt
    31321 tatagcttgt tacacttagc tggttatgat ttatctgtag aagatttaaa gaacttccgt
    31381 caatggggtt ctaaaacacc aggtcaccca gaagtgaatc acacagacgg tgtcgaagca
    31441 accacaggac ctcttggtca agggatcgca aatgccgttg gtatggccat ggcagaagct
    31501 cacctagcag ctaaatttaa caaaccaggc tttgacatcg ttaatcacta cacctttgct
    31561 ttgaatggtg acggtgacct tatggaaggg gtcagccaag aagcagcaag tatggcagga
    31621 catttaaaac ttgggaaatt ggtcttgcta tatgattcaa acgacatctc tcttgatggt
    31681 ccaacctcta tggctttcac agaagatgtg aaaggacgtt tcgaagctta cggttggcaa
    31741 catatccttg tgaaagatgg aaatgattta gaagaaattg cagctgctat tgaagcagct
    31801 aaagctgaaa ctgagaaacc aaccatcatc gaagtcaaaa ccattattgg ttttggtgcc
    31861 gaaaaacaag gaacttcagc tgttcacggc gctcctcttg gagcagaagg cattgctttt
    31921 gccaaaaaag cttaccaatg gactcatcaa gattttgaag tgcctgctga agtaacagag
    31981 cgctttgctc aaggtcttca agcgcgtggt gaaaaagcag aacaagcctg gaatgacttg
    32041 tttgcagttt atgaagcaga atatccagaa ttagcagcag aataccaaaa agcctttact
    32101 aacgaagctg ctcaagtaga acttgaagcg cacgaacttg gtagttcaat ggcgagtcgt
    32161 gtatccagtc aacaagccat ccaacaaatc tcagaacaag tagcctcttt ctggggtgga
    32221 tcagcagatc tttcagcatc taacaacaca atggttaagg ctgaaacaga tttccaacca
    32281 ggtcactacg aaggccgtaa catctggttt ggcgttcgtg aatttgccat ggctgcagcc
    32341 atgaacggta ttgcccttca cggcgggaca cgcgtttacg gcggaacctt ttttgtcttc
    32401 tctaactatc ttcttccagc tgttcgtatg gcagcgctcc aaaacttgcc aacagtttat
    32461 gtcatgacac atgattctat cgctgttggt gaagatgggc caacgcacga accaattgaa
    32521 caattggcaa gtgttcgctc aatgcctaac tttaacgtta ttcgtccagc agacggtaac
    32581 gaaacaaatg ctgcttggaa acgtgccatt gctgaaacag atcgcccaac catgcttgta
    32641 ttgacgcgtc aaaaccttcc agtgcttgag ggcacaaaag aattggcaga agatggtctt
    32701 aacaaaggag cttacatctt gtcagaagcc aaaggcgacc ttgaaggtat cttgattgca
    32761 acaggttcag aagtgaaact ggcaatggat actcaagaag ctcttgaagc agaaggcatt
    32821 catgtgcgtg tcgtatctat gccatcacaa aatatctttg acgaacagtc agcagagtat
    32881 aaagaaagca tcctaccagc agctgtgact aaacgtctcg ctatcgaagc aggttcaagc
    32941 tttggctggg caaaatatgt tggcctagca ggaaaaacat tgaccattga cacttggggt
    33001 gcttcagctc caggaaatcg tatcttcgaa gaatatggtt tcactgtggc aaatgcaact
    33061 gagttataca aatcactcta ggttacacat cccaaacaga gtaaatcaaa tgatttactc
    33121 tgttttttta aaataaaata accagttctt tcttttcctc aaactgccta atacacttct
    33181 tttgataaga agtcattgac aaaacgattc ataaaaatta taatttaatt gttattgtat
    33241 tagcgtaaaa gtacaaaaaa taaaggagta taaatggaaa agaaacgaca gcgcttatac
    33301 gatgtgatta gacaggctta tgattatcca gaaaaccgtg aaaatgtggc cttgtcgcag
    33361 ctacttttag cagcttctaa tagattaatt aaacacagta acccacttct tgttgcctac
    33421 caactaaacc aagatgtgga taattactta cttgataatg acattttact tccaaaatcg
    33481 ctttgccgtt ttaagcagtc attggaaaag tatattttag aggaagcctc atcagtctag
    33541 ggagaaaaag agaagaaaag gaaaaaggat accatgatag aatttaaaca tgtttcaaag
    33601 ttgtatggcg acaaagaagc tctcagcgat ttaaacgtca ccatcaatga cggtgaaatt
    33661 tttggcctta tcggacataa tggtgcaggt aaaaccacga ccatcagcat tttgacttct
    33721 attatcgaag cgagttatgg cgaagtattt gtggatggtc aacttctgac agaaaataga
    33781 gaagctatca aaaaacaaat tgcttatgtg ccagattctc ctgatatttt cttgaatcta
    33841 actccaaatg agtattggca gtttttagct aaaatttatg gggtgtcaga tgaggaccga
    33901 gaagaacggt tagcacagct gacaacactt tttgaactga aggaagaagt taatcaaacc
    33961 attgatagtt tctcacatgg gatgcgccaa aaagtgattg tgattggtgc tcttgtatca
    34021 aatcctaaca tctggatttt ggatgaacca ttaacaggtt tggatcctca ggcttctttt
    34081 gaccttaagg aaatgatgaa agctcatgca gcttctggac atacggtctt attttcgact
    34141 cacgtgctat cagtcgctga gcagttatgt gatcgtatcg ggattctaaa aaaagggaaa
    34201 ttgatttttg ttggaacgat tgatgagttg aaagaacacc atcctgacaa agacttggag
    34261 agcatctacc ttgaactcgc aggtcgtaag gcgcaagaag aggggtgatg ggatgaattg
    34321 gtctactatt tgggaactta tcaagataaa cattctttat tccaatccac aaagtttagc
    34381 taacctcaaa aagcgtcagg aaaaccaccc gaaagaaaac ttcaaagcct ataaaagcat
    34441 gatgaggcaa caggccttaa tgattgccat gtttttggtg atttaccttt ttatgtttat
    34501 tggtgtcgat ttcagtcatt atccaggact tttctccttt gacgttgcta tgttttttat
    34561 catgtcaacc ttgacagcct ttagctctct ttacaccatt ttttatgaaa gtaatgactt
    34621 aaaactttat attcacttac cagtaacgtc agaagaactc tatattgcaa aaattgtctc
    34681 gtcgctaggg atgggtgctg tctttttgat gcctcttatc tctcttctct taattgccta
    34741 ttggcaacta ttgggaaatc ctttgtctat tttagtagct attgtgctct ttttagtgtt
    34801 actagtgagt tcgatggtat tagctattta tatcaatgct tgggtgggca aaatcattgt
    34861 aaggagtcgc aagcggaaac tcatttcaac tatcatgatg tttgtctcaa cttttggagc
    34921 tttcgtctta atttttgcga ttaatattag taacaacaag cgtacgatga cggatggcgt
    34981 atttactgat tacccaacta ttccgtattt caaaggattc tatgatgtta ttcaggcacc
    35041 gttttctact gcggccctcc ttaacttttg gttgccattg ctactgattt tagctatggt
    35101 atatggtatt gtcacaaaag tgatgccaac ttattatcgt gaggcttttt atattagtaa
    35161 cgagaacaaa gccaagcaaa ctaaaaaacc agttaatcgt cctcatcaga atcaatcact
    35221 gacgcagttg ttgcgaaaac atcatctttt aacgttacaa aatgcaactt tactgacaca
    35281 aacctatctc atgcccctga tgtatgtgat gctttttatc ggtccaagtt tgtcacgtgg
    35341 cacaggtttc tttaagcata tttccccaga ttactttggg gtggccttat tatttggggt
    35401 cagtttgggt gtcatgtgtg caacaccaac cagctttatt ggagtaggta tttcacttga
    35461 aaaggataac tttaccttta ttaaaagctt accaataacg ttaaaaaaat tcttgctgga
    35521 taaattttgc ttgctcgtcg gtctgcaact gattgtgcct atggtgattt atcttgtgtt
    35581 tggtctcttt gtgttgcacc tgcatcctct tctaacgatt gctttttgtc ttggctacgc
    35641 cctgtctctg attgtgcaag gggaattgat gtatcgtcgt gactaccggc ttctggattt
    35701 aaaatggcaa gacatgacac aactctttac cagaggaaat gggcagtggc taacaatggg
    35761 ccttatcttt ggtaatttaa tagtcacagg tgtagtaggt tttggagctg ttatcattgc
    35821 caatattatc caacaacctc tgttgattag tattctattg agttgtctga cactaatggt
    35881 tttagggatt gctcacttat ggattcaaaa aaccttctgg aaaagcttag agaagcttta
    35941 acgaagagag atcattattc ggaaaaacag tcctctgggg ctgtttttat gctataattt
    36001 gggtatgatg aaacgacaat ttgaagatgt aacacgtatc gtaattaaaa ttggcactag
    36061 ttcattagtg ctaccaacag gtaaaatcaa tttagaaaaa atcgaccaac tggcttttgt
    36121 gatttcaagt ttgatgaata agggcaagga agttatttta gtatcatcgg gagcgatggg
    36181 atttggacta gatattttga aaatggaaaa acgtccgacc aatcttgcta aacaacaggc
    36241 agtttccagt gttggtcagg tggccatgat gagtctttat tcacaaattt ttgcacacta
    36301 ccaaaccaac gtgtcacaaa ttttattgac gagagatgtg gtggttttcc cagaaagtct
    36361 agccaatgtt accaatgctt ttgaaagtct gattagtttg ggcattgtgc cgattgttaa
    36421 tgaaaacgat gcggttagtg tggatgaaat ggatcatgcg actaaatttg gagataatga
    36481 ccgtttatct gctgttgttg ctggtattac aaaagctgat ttattaatca tgttgtctga
    36541 tattgatggc ctttttgata aaaaccctac tatttatgag gatgcgcagt tacgcagtca
    36601 tgttgcagtc attacgcaag aaatcattgc gtcagcaggt ggtgcgggca gtaaatttgg
    36661 cacaggtggg atgcttagca aaattcagtc tgctcagatg gtatttgaaa acaaaggaca
    36721 gatggtcttg atgaatggag ctaatcccag agatatttta agagtgcttg aagggcaacc
    36781 actaggcacc tggttcaagc agatagaaga ggtaacacat gactgatatg agaaggttag
    36841 ggcaaagggc aaaacaagct agtcttttaa tagcaccttt gtcgacgcaa attaaaaatc
    36901 gctttttaag caccttagca aaggcattag ttgacgatac acagactctc ttagccgcta
    36961 accaaaaaga tttagcgaat gctaaagaac atggtatttc tgacatcatg atggatcgct
    37021 tgcgcttaac ttcagaacgg attaaagcga tagcacaggg agttcaacag gttgctgact
    37081 tagctgatcc aatcggacaa gtcattaagg gctatactaa cctcgatggc cttaaaatcc
    37141 tccaaaagag agttcctcta ggggtgattg cgatgatttt tgagagtcgc ccgaatgttt
    37201 cagtggatgc ttttagtctg gcgttcaaaa ccaataatgc catcatccta aggggaggaa
    37261 aagatgccct tcactcaaat aaagctttgg tgaagttgat ccgacaaagt ctggaaaaat
    37321 cagggatcac cccagatgct gtccagctag ttgaggatcc ctcacatgct gttgctgaag
    37381 aattgatgca ggcaacggac tatgtggatg tgttgattcc acgtggtggt gctaaattaa
    37441 ttcagaccgt taaggaaaaa gcaaaagtgc ctgtcatcga aacaggtgtg gggaatgtgc
    37501 atatctatgt ggatgctcaa gctgacttag atatggctac taatattgtt atcaacgcta
    37561 aaacaaagcg cccaagtgtt tgcaatgcag ctgagggatt ggtaattcac gaagctgtag
    37621 cagcaaggtt tattcctatg cttgaaaaag cgatcaatca ggttcagcca gtagaatggc
    37681 gggcagatga caaggctttg ccgttatttg agcaggcagt gcctgcaaaa gcagaggatt
    37741 ttgagaccga atttttagat tatatcatgt ctgttaaggt tgtttcctct ttggaagaag
    37801 ctatttcttg gataaatcag catactagtc accattccga agctatcatc acaagggata
    37861 ttaaggcagc agagaccttt caagatttag tggatgctgc tgctgtttat gtcaatgctt
    37921 caacacgatt taccgatggt tttgtttttg ggcttggtgc tgaaattggg atttctactc
    37981 aaaaaatgca cgcccgtggg ccaatgggct tagaagcctt gaccagcact aaattttata
    38041 ttaatgggga tggacatatc agagaataag ctactagaag gtcccttaat gactaatgaa
    38101 agagaaaaag aagaagcaaa ggttgcttta gaagaacttt tacatcagat tcaggtgatt
    38161 catttggaat tgaaaattgt ccgaaattgg ggctattttg atctttttgg agacggtatg
    38221 cttgtcagct ggatcaaacg tcgaaaaatg agcaaggtca accaattatt aatagactta
    38281 caagttactc ttcaaaactg ccgaagatag ctggcagacg tcgataggct attattagct
    38341 gacttaccga atatgagttg ggataaaatc tttgatattt ggttagacaa cctgctgaca
    38401 gattgttgtg tgattaaaga agctaaaaat tttttacaag tagttgaaat tctagaggca
    38461 gcggctaagt cagtgcttgc taagttggct gatagcgaac aaaaagaaca agattagaga
    38521 agaggaagat tcccacgaga atgaaatcct tttctcttac tttttcattt ctaaaccttt
    38581 tgaagtatgg tacaattaaa gttatgacaa aagaatttca tcacgtgacc gtactccttc
    38641 acgaaacagt ggacatgctt gacataaagc ctgatgggat ttatgttgat gcgacgctag
    38701 gtggctcagg ccactcagct tatttgttgt ccaaacttgg tgaagaaggg cacctctatt
    38761 gttttgacca agaccaaaag gctattgaca atgcacaagt taccctcaaa tcttatattg
    38821 acaaaggaca ggtaactttt attaaagata attttagaca cctcaaagca cgtttaacag
    38881 cgcttggagt tgatgaaatt gatggtatct tatacgacct tggtgtttcc agcccgcaat
    38941 tggatgaaag agaacgaggg ttttcttata aacaagatgc tccattggat atgcgcatgg
    39001 atcgtcagtc gctcttaaca gcttacgaag tggtgaatac ctatccattc aatgatttgg
    39061 ttaagatttt cttcaaatat ggtgaagata aattctccaa gcagattgct cgaaaaattg
    39121 aacaagcaag agctattaag cctattgaga caacaacaga gttggcagaa ttgattaagg
    39181 cagcaaagcc agctaaagag ttgaagaaaa aaggccaccc tgctaaacag atttttcaag
    39241 ctattcgcat tgaagtcaat gatgaattgg gagcggccga tgaatctatt caggacgcta
    39301 tggaattatt agcccttgat ggtcgtatct cagtcattac cttccattct ctggaagatc
    39361 gcctaaccaa gcagttgttt aaagaagcta gtacggtgga tgtgccaaaa gggcttcctc
    39421 taattcctga agatatgaaa cctaagtttg aacttgtttc acgtaagccg atcttaccta
    39481 gtcattcaga gttaacagct aataaaaggg cacactcagc caagctacgt gttgccaaaa
    39541 aaattcggaa ataagcctat gacaaatgaa aaaagaactc aggcagttac aaatgccttg
    39601 caaaaacgaa taaaaacttt ctctagaatt gagaaggctt tttatactgc tatcattgta
    39661 acagcgatta cgatggcagt aagtattatt tacctgcaaa gtcgtaagtt gcagttacaa
    39721 caagaaatca ctagtttaaa tagtcatatt tcggatcaaa aactagagct taataatgcc
    39781 aagcaagaag taaatgagtt atctcgccgt gatcgtatta tagatattgc tggtaaagca
    39841 ggcttaagca accgtaataa taatatcaag aaagtcgagt aatctatgaa aaaatggcaa
    39901 aaatatgttt tagattatgt tgtgcgcgat aggagaactc cagtcgaaaa tcgcgttcga
    39961 gttggacaaa atatgatgct cttaactatc tttattttct ttattttcat tattaatttc
    40021 atgattatta ttggaacaga tcaaaagttt ggagttagtt tgtcagaagg ggcgaagaag
    40081 gtttatcaag aaaccgttac gatccaagct aagcgtggga ccatttatga tcgaaatggt
    40141 acagctattg cagtggattc tacgacttat agcatatacg caattttgga taaatcattt
    40201 gtctcggctt cagatgaaaa gttatatgta caaccttctc agtatgaaac agtagctgat
    40261 attttaaaaa agcatctggg aatgaaaaaa acagatgtaa ttaaacagct taagcgtaaa
    40321 ggacttttcc aagtctcgtt tggaccgtca gggtctggca tttcatatag tactatgtct
    40381 actattcaaa aggctatgga agatgccaaa atcaagggaa ttgctttcac aactagtcct
    40441 ggtcgtatgt atccaaacgg gacatttgct tcagaattta taggcttggc atctctaaca
    40501 gaagataaaa agacaggtgt taagagttta gttggaaaaa caggtctaga agcttctttt
    40561 gataaaattt tatcaggtca agatggtgtt attacttatc aaaaagatcg aaatgggacc
    40621 acactcttgg gtacaggtaa gactgtcaag aaagctattg atggcaaaga tatttacaca
    40681 acgctatctg agcctatcca gaccttctta gaaacccaga tggatgtttt tcaagccaaa
    40741 tcaaatggtc agttggccag tgcaacactt gttaatgcta aaactggtga aattttggca
    40801 acaacacaac gccctactta taatgctgat actctgaaag gacttgaaaa tacgaactac
    40861 aaatggtaca gtgcacttca tcaaggaaat tttgagccag gctcaaccat gaaagtgatg
    40921 actctggcag cggctattga tgataaagtt ttcaacccaa acgaaacctt tagcaatgct
    40981 aatggtttaa caattgcaga tgctactatt caagactggt ctattaatga aggcatttcc
    41041 acaggacagt acatgaatta tgcacaagga tttgccttct caagtaacgt tgggatgact
    41101 aaacttgaac aaaaaatggg taatgcaaaa tggatgaatt atttgacgaa gttccgcttt
    41161 ggttttccta ctcgttttgg tttaaaagat gaagacgcag gtatatttcc ttctgataat
    41221 atcgtgactc aagctatgag cgcttttggt caagggattt ctgtaaccca gattcaaatg
    41281 cttagagcct ttactgctat ttctaataat ggtgagatgt tagagccaca atttatcagt
    41341 caaatttatg atcctaacac agcaagtttt agaacggcaa ataaagaaat tgttggaaaa
    41401 cctgtatcaa aaaaagccgc tagtgaaaca agacaataca tgattggtgt aggaacagac
    41461 cctgagtttg gaacactcta ttcaaaaaca tttggaccaa ttattaaagt gggtgattta
    41521 cctgttgctg ttaaatcagg aacagcacaa attggttcag aagatggaag tggttatcaa
    41581 gatggtggat tgactaacta tgtctattcg gttgtggcaa tggtgccagc tgataaacca
    41641 gactttttga tgtatgttac tatgactaaa ccacaacatt ttggtcccct tttttggcaa
    41701 gatgtggtta acccagtatt ggaagaagca tacttaatgc aagatacact aactaagcca
    41761 gtagtatcag atgctaatcg tcaaacaact tataaattac caaactttgt aggaaagaat
    41821 cctggtgaga catcaagcga gttgcgtcga aatcttgtcc agccagttgt ccttggtact
    41881 ggcagcaaga tcaaaaaagt atcgcatcag cccggtcaaa cgttaacaga aaaccaacaa
    41941 gttctcatat tatcagaccg ttttgtggag gtaccagaca tgtatggctg gacaaaatcc
    42001 aatgttaaaa cctttgctaa atggactgga atagacatca gctttaaagg aacagattct
    42061 ggtcgtgtta tgaaacaaag tgttgatgtt ggtaagtcct tgaaaaaaat aaaaaaaatg
    42121 accattactt taggagatta acatgttttt aacccttatt gcagcaatca tatcctttat
    42181 ggtatctgct ttcacaatgc cttattttat caaattctat caattgaaaa aaattggtgg
    42241 gcaacaaatg catgaagatg tcaagcaaca tttagctaaa gctggaacac cgactatggg
    42301 gggaactgtt tttcttttag tagctacggc agtatcgtta ttggttagtc tattttcgat
    42361 caaaaacact caaagtttag ctttgatttc aggcatttta tcaattgttg tcatttacgg
    42421 gattatcgga tttttggatg attttttaaa aatttttaaa caaatcaatg aaggattgac
    42481 agccaaacaa aagttagcct tgcagttggt tggaggattg atgttctatt ttttacatgt
    42541 tagtccaagt ggcatttcct ctattaatgt gtttggttat cagctgccgc tgggaatttt
    42601 ctacctattc tttgttttgt tttgggtggt tggtttttca aatgccgtta atttgacaga
    42661 tggtatcgat ggcttagctt ctatctcagt ggtgattagt ctagtgactt acggcgttat
    42721 tgcctacgtt cagagtcaat ttgatgtttt gttactgatt ggagcaatga ttggagcctt
    42781 gcttggcttc ttctgcttta atcacaaacc tgctaaagta tttatgggag atgtaggtag
    42841 tttagccctt ggagctatgt tggctgccat ttctattgcg cttcgtcaag aatggactct
    42901 cctgattatt gggatcgttt atgttcttga aacaagttct gtgatgttgc aagtgtccta
    42961 tttcaagtac accaagaaaa aatatggaga aggtcgtcgt atttttagaa tgacgccttt
    43021 ccaccaccat ttggaattgg gaggcttgtc tggtaaaggc aagaagtggt cagaatggca
    43081 ggtagatgct tttttgtggg gagtaggaag tctagctagt ttactagttt tagctatctt
    43141 atacgtgttc taagggggat tatcaagaat agtcaccaat agcattttga ttaacatcat
    43201 caataagaga gtggagatga cccacaaatg ctgattttgt gagtgttgtc ccactccttt
    43261 tgcttgtctc taacaataat gatataatat ggaagaaaac gaggtaaaac atgtcattta
    43321 aagattatca ttttaagcaa tacgttcagc aagcgctgga agaaattggc tttgtcaatc
    43381 caacagaggt ccagaagcgg ctgattccta ttgttaactc cggtcgtgac ttagttgggg
    43441 aatcaaaaac aggttcaggg aaaacccaca cctttttgtt gcccattttt gaaaaacttg
    43501 atgaagctaa agcagaagtg caagtcgtta ttacagcacc tagccgtgaa ttagcaactc
    43561 aaatttttga tgcttgtaaa cagattgcta agcacttcca agaagagatt cgtttggcca
    43621 actatgttgg aggcacagac aaactccgtc agattgaaaa actaaaaggc agccagccgc
    43681 atattgtcat cggaacccca ggtcgtattt atgacttggt caagtctggt gatttagcga
    43741 ttcataaagc aacgaccttt gttgtggatg aggccgatat gaccatggac atgggctttt
    43801 tggatacggt agataagatt gcagcttcgc ttccaaaatc tgttcagatt ttggtgttct
    43861 cagcaactat tccacagaaa ttacaaccct ttttgaaaaa atacttgacc aatcctgtta
    43921 ttgaacaaat caagacaaag accgttatcg cagataccat tgataactgg cttgtgtcga
    43981 caaaagggcg tgataagaat ggtcaactgt tagagattct caaaaccatg cagccttata
    44041 tggctatgct ttttgtcaat accaaggaac gcgcagatga tttgcatgcc tttttaacag
    44101 ctaatggctt aaaagtggct aagattcatg ggggcattcc tccaagagaa cgtaagcgca
    44161 tcatgaacca agtcaaaaaa ctggactttg aatacattgt tgccacagac ttagcagcgc
    44221 gtgggattga cattgaaggg gtcagccatg tgatcaatga cgccatccca caagacttgt
    44281 ctttctttgt ccatcgtgta ggccgaacag gtcgaaatgg tatggcagga actgccatta
    44341 cactttacca gccaagtgac gactcagata tcaaagagct ggaaaaaatg gggattgcct
    44401 ttacaccgaa ggtgctcaaa aatggagaat ttcaagatac ctatgaccgt gatcgccgtc
    44461 aaaaccgtga aaaagcctat caaaaattag acacagaaat gattggtttg gtcaaaaaga
    44521 aaaagaaaaa agtcaaacca ggttacaaga aaaaaattca gtgggcggtt gatgaaaaac
    44581 gtcgcaaaga acgtcgtgct gaaaatcgtg ctaagggccg cgctgagcgt aacgccaaaa
    44641 aacaacattt ttaagaacaa agagcaagca agtgatcaat tggcatctaa tcttgctttt
    44701 tttacacctg ctacttgtat tgtttaccat ttgttaacac aaaaagtgat catagctttt
    44761 gcttattgct ataatacgga tttactattt tacatgtggg tctaagagtg ttagaatatt
    44821 aagacaaaca aatacaaagg ggaaatacat gacatcagtg tttttaacca gtggttgggc
    44881 tttttatgat tacctcatct caccgattcc acatggaaaa ctttttagtt ggcatgctgt
    44941 ttttgatgcc attccaaata ttatccaacg gcttccaatt acgcttggtt tgacactatc
    45001 aggagcaacc tttggcttgg ttttggcttt gatttttgcc cttgtcaaaa ttaataaagt
    45061 gaagctacta taccctattc aagctatttt tgtgagtttc ttgcgaggaa ctcctatttt
    45121 ggtacagttg atgttgacct actacggtat tcccctcttt ctaaaatttc tcaatcagaa
    45181 gtatggcttt gattggaatg ttaatgcaat tccggcttct atttttgcca ttacagcctt
    45241 tgcttttaat gaagcagcct acgctagtga aactattcgg gcagccattt tatcagtaga
    45301 tacaggtgaa attgaagcag caaaaagtct aggcatgact tctgtgcagg tttaccgtcg
    45361 tgtcattatt cctaatgcaa ctgttgtggc tattccaacc ttgattaatg ggttaattgg
    45421 cttaactaaa ggaacatcgc ttgcctttaa tgcagggatt gtcgaaatgt ttgcccaggc
    45481 tcaaattcta ggcggatcag attaccgtta ttttgaacgt tatatctctg ttgctcttgt
    45541 ctattggtct atcagtattt tgatggagca agtaggtcgc ttgattgaaa acaagatggc
    45601 catcaaagca ccagaacaag ctagaaatga aaagttagga gaattgcgtt gatgattaca
    45661 attagaaatt tgagcaagac attctcaggg caaaaagttt tagatagttt ggctcttgat
    45721 attgaaaaag gtcaagtgat tgctttagtt ggtgcttcag gagcagggaa atcgaccttc
    45781 cttcgtagcc taaactattt ggaaaaacca gattcaggat ccattagcat tggtgacttt
    45841 acggttgatt ttgagaccat taccacagag caagtcctta ttttacgtcg gaaacttgcc
    45901 atggtttttc aacaatttaa tctttttgag cgtcgtacag cccttgagaa tgtcaaagaa
    45961 ggtcttaagg tggtcaaaaa actgtctgac caagaagcga ctaagctggc gcaagcagaa
    46021 ttggctaagg ttggtttagc agatcgaaaa catcattacc cacgtcattt atcaggggga
    46081 caaaaacaac gggtagcttt agcaagagct ctggctatga aaccagatgt tttattacta
    46141 gatgagccga cctcggctct tgacccggaa ttagttggcg aagtggaaaa gtctattact
    46201 gatgctgcta aatccggaca gaccatggtt ttagtgagtc atgacatgaa ttttgtttac
    46261 caagtggcag atagagtact ctttttagat caagggaaaa tcttggaaca aggaacacct
    46321 gaagaagtct ttagacatcc tcaaaaagaa cgaaccaaag aattttttgc aagctattca
    46381 aaaacataca tttaatgata ttcttttgtt ataataaaaa ggtctaggta ttcctgggct
    46441 tttcttgtta tgttttttag aaaaacttat gggaaagaaa taatgactag gtggaaatat
    46501 gttagtagaa atctttagcc tttatgtaaa aggtctcatt ctctcaagcc ttactatgat
    46561 tttaatttgt ttaatatgga ctttatggcg agcaaaaacc aaaagagata aaacattgat
    46621 tgaaagacaa gcttttcttt atgagatgct aatggttgct attttaacca tcccaatttt
    46681 atcgtttgct tttatgagta ttttggttgt cataaaatcc taatcattgc aagaagggat
    46741 tatcattgct aaaatattgg caagagtaga ttaaaggaga aaaatcatgt acgatacatt
    46801 aattattggt tcaggaccag caggaatgac tgccgcttta tacgctgcta gaagtaattt
    46861 atctgttgcc attattgaac aaggggcacc tggtggtcag atgaacaaca cttttgatat
    46921 agagaattac ccgggatatg atcatatatc tggaccagaa ttagctatga aaatgtacga
    46981 acctttagag aagtttaacg ttgaaaacat ttatggtatt gttcaaaaaa tagaaaattt
    47041 tggtgactat aagtgtgtgt tgactgaaga tgctagttat gaagctaaaa cggttattat
    47101 tgcaacggga gctaaatacc gtgtactagg tgttccaggt gaagaatact atactagtcg
    47161 aggagtttct tattgtgcag tatgtgatgg tgcgtttttc cgtgaccagg atcttttggt
    47221 cgttggtggt ggtgactcag cagttgaaga agctatttat cttactcagt ttgctaaaaa
    47281 ggtaaccgtt gttcatcgcc gtgatcaact aagagctcaa aaaatactgc aagatcgtgc
    47341 ttttgccaat gataaggtcg actttatctg ggattcagtc gtcaaagaaa ttcaaggcaa
    47401 tgatattaag gtctccaatg tcctaatcga aaatgtgaaa acaggccaag tcactgacca
    47461 cgcttttgga ggtgtgttta tctatgttgg tatgaatcct gtgacaggta tggtaaagga
    47521 tctagaaatt accgatcaag aaggttggat tatcacagat gatcatatga ggacaagtat
    47581 tcctggtatt tttgcgattg gagatgttcg tcaaaaagac cttcgccaaa tcacgacagc
    47641 tgttggagat ggtgcaattg cagggcaagg tgtttatcac taccttgaaa gtttcccttc
    47701 ataaggttaa aactccttca tttttccttg tctttccctt aaaaaagcta aattgcataa
    47761 gacctttaag aaatataaag aacagttttc cttaatatct ttcctagata aatgttgaaa
    47821 attttctgaa aaaaggatat aataaatagt attttagtga atggagttta gaatgtctat
    47881 aaaagaacaa acagacaata acgaactaga aaatggaatg gtaaggggct tagaaaatcg
    47941 tcatgtgcag ttaattgcga ttgcaggaac gattggaaca ggtctctttt taggggcagg
    48001 acgctctatt gccctaacag gaccatctat tatttttgtg tatatgatta caggtgcctt
    48061 catgtttatg atgatgcgtg ccatcggtga aatgctctat tatgatccag atcaacacac
    48121 ctttatcaat tttatttcca aatacattgg tccaggctgg ggttattttt cgggtctatc
    48181 ctattggatt tcccttattt ttattggaat ggcagaaatc acagcagtag gtgcttatgt
    48241 gcaattttgg ttcccaagct ggccagcctg gttgattcag ttggttttct tagtcttact
    48301 tagttcgatc aacttaattg ctgtgcgcgt ttttggggaa acagagtttt ggtttgccat
    48361 gattaagatt ttagctattt tagctttgat tgcaacagct attttcatgg tattgacagg
    48421 ttttgaaact cacacaggcc atgctagcct ttccaatatt tttgaccatt tttccatgtt
    48481 cccaaatggg aaactaaagt tctttatggc cttccaaatg gttttctttg cttatcaagc
    48541 tatcgaattt gtgggaatta ccacttctga gacggctaac ccaagaaaag ttctaccaaa
    48601 ggctattcaa gaaattccaa cccgtattgt gatcttttat gtgggagcct tggtctctat
    48661 tatggcaatt gtgccatggc atcagttacc agttgatgaa tctccttttg tgatggtgtt
    48721 caaattgatt ggtattaaat gggcagcagc cttgattaac tttgtggtct tgacatcagc
    48781 agcgtcagcg cttaactcaa ccctttattc aaccggtcgt catctctatc agattgctaa
    48841 tgagactcca aacgctttga ccaatcgctt aaagattaat actttatctc gacaaggggt
    48901 gccaagtcgt gccattattg cttcagcggt tgtggttggt atctcagctt tgattaatat
    48961 cctaccagga gttgcagatg cgttctcact cattacggcc tcctcatcag gtgtatatat
    49021 tgctatctac gccttaacca tgattgctca ctggaagtac cgtcaatcta aggactttat
    49081 ggcagatggt tatttgatgc caaaatataa agtgacaacg cctttgaccc ttgctttctt
    49141 tgcttttgtc tttatctctc ttttcttaca agaatccacc tatatcggcg ccattggagc
    49201 aacgatttgg attattgttt ttgggattta tagcaatgtc aaatttaaat aataattagc
    49261 gatatagaac tgaatccatt aactctttct tatttgagga tgtcatgatg gatcagttct
    49321 ttttttactt tagcgataac tcaagttatt tctaaaccct agccaaagag tcctttttaa
    49381 aatatgttat aatagtaaca ataattcaaa ggagtcccct ttatgtataa agatgatagt
    49441 ttaactctcc atactgattt gtatcagatt aatatgatgc aagtttattt tgagcagggc
    49501 attcataatc gtcatgcggt gtttgaagtc tatttccgca aggaaccttt taacaatggt
    49561 tatgctgttt ttgccggttt acagcgcatg gtggagtatc tagagcaatt tcaattttca
    49621 gaaacagatt tggcttacct tgaagaactg ggttatccag gaaacttttt gacctacctt
    49681 aaagagttac gattagaatt gaccatccgt tcagctaaag aaggagattt ggtttttgct
    49741 aatgagccga ttgtgcaagt agaaggccct ctaggtcagt gtcagttggt cgaaacagct
    49801 cttttaaaca ttgttaactt ccagaccttg attgctacaa aggctgctcg tattcgttct
    49861 gtgattgagg atgaacccct tttggaattt ggtacccgtc gtgcacagga actagatgct
    49921 gcgatttggg gaacccgcgc agcgatgatt ggtggagcag atgcaacaag taatgtgaga
    49981 gcaggtaaac gctttgatat ccctgtttca ggtacgcatg ctcatgcttt agtgcaagcc
    50041 tatggtaatg attatgatgc tttcatggct tatgctaaga cccataaaga ttgtgtattt
    50101 cttgttgaca cctatgacac acttaaagta ggtgtgccaa cagctatccg agtagccaaa
    50161 gaaatgggtg acaagattaa ttttctaggt gttcgattgg actctggtga cttggcttac
    50221 ctctcaaaaa cagtccgtca gcagttagac gacgctggtt ttaccgaggc taagatctat
    50281 gcttctaatg acctcgatga aaataccatc ctcaacttaa aaatgcaaaa ggccaaaatt
    50341 gatgtttggg gtgttgggac caaactgatc acagcctatg atcagcctgc cttaggtgct
    50401 gtttataaga ttgtctctat tgagcaagaa gatggtagca tgcgtgatac gatcaaatta
    50461 tcaaataatg cagaaaaagt atccacacca ggtaaaaaac aagtttggcg catcaccagt
    50521 cgtgaaaaag gcaaatcaga aggcgactat atcacgttta ctgatattaa tgttaatgaa
    50581 ttaactgaga ttgagatgtt ccatccgacc tatacctata ttaagaaaac cgtcagaaat
    50641 tttgatgcaa ttcctctctt agttgatatt tttgtcaagg gagaattagt ttaccagcta
    50701 ccgaccttat ctgaaatcaa gacttatgct aaaaaagaat ttgacaagct ttgggatgaa
    50761 tacaagcgtg tcttaaatcc tcaagactac ccagtagact tggcacgaga tgtgtggcaa
    50821 aataagatga ccttgattga taacattcgt aaagatactc atggaaagag tgaatgacat
    50881 gacattacaa gaagagatca ttcgacaatt aggcgtcaag gctagcattg atccacaaga
    50941 agaaattcga aaaaccgttg attttttaaa agcttatttg cgaaagcact catttttaaa
    51001 aacttatgtc ttaggcattt ctggaggtca ggattctacc ctcgcaggaa agctagccca
    51061 gatggccatt gcagagttac gggaggaaac aagtgatcaa gcttaccaat ttatagctgt
    51121 tcgcttgcct tacggggttc aggctgatga agcagatgcg caaaaagcct tggcttttat
    51181 tgcaccagat cagaccctta ccattaatat caaggcagct gtggacggtc aagtagaggc
    51241 tctacaagca gcaggcgttg agatttctga ttttaacaaa ggtaatatta aagcacgtca
    51301 gcgaatgata agtcaatatg ccattgctgg ccaaatggct ggtgctgtta ttggaacgga
    51361 tcatgcggca gaaaacatca ctggtttctt tactaaattt ggtgatggtg gagcagatat
    51421 tcttcccctc tttaggctta acaaacgtca aggaaaggct cttttgaaag ttttaggagc
    51481 agatgctgct ttgtatgaaa aagttccaac tgctgattta gaagatcaaa aaccaggctt
    51541 ggctgatgag gttgccctag gggtgaccta tcaagacatc gacgactatt tggaagggaa
    51601 gctgatttcc aaagcagctc aagcaactat tgaaaaatgg tggcataaag gacaacacaa
    51661 gcgtcacctg ccaatcacca tttttgatga tttttggaaa taagaaatga tgttcaaaga
    51721 gtaaaaaaag aaaacaaaaa aagttaggtt atcctaactt ttttcttgaa gaaaatcaaa
    51781 taaggtctat aatagaagta atcctttaga atagaatggc tggataataa ccatcttgcc
    51841 aatattattt caggtgaagc ttatcttaat attttaggag gacaataatg tcagcattaa
    51901 cagagacatt tacagaacaa ttatttgctc attacgaagc caatgcaaaa ttttcagcta
    51961 ttgagaatgc cgttacgcat aatggtttgc taaaatcatt agaaacccgt caaagtgaag
    52021 tggacaatga ttttgtgttt tctattgatt taactaaaga taaggtatct aaccaaaagg
    52081 catctggtcg ctgctggatg tttgctgcct tgaacacttt ccgacacaaa ttaatcacag
    52141 agtttaaatt agaaaatttt gaactgtcac aagcccatac tttcttttgg gataagtatg
    52201 aaaaagctaa ctggtttatg gagcaggtca ttgctacagc tgaccaagaa ttaaccagtc
    52261 gtaaagttaa gtttctcttg gatgttccac aacaagatgg tggccagtgg gacatggtgg
    52321 tttccttgtt tgagaagtat ggagtggtgc caaaatctgt ttacccagaa tcaatctcat
    52381 caagtaacag tcgtgaatta aaccagtatt tgaataaatt attgcgccaa gatgctcaaa
    52441 ttcttcgtga cctgattgca tcaggtgcta aggcagacca agttgaggat cgaaaagcag
    52501 agcttcttca ggaaattttt aacttcttgg ccatgaccct tggtttacca cctcgtcatt
    52561 ttgattttgc ctaccgtgat aaagatgacc actatcatgt tgagaaaggt ttgacaccgc
    52621 aagcgtttta cgacaaattt gttggcttaa agctttctga ttatgtgtct gttattaatg
    52681 cgccaactgc tgataagcct tatggcaaat catacactgt tgagatgcta ggaaatgtgg
    52741 tcggtagtcg tgaggttcgc tacctcaacc ttgatatgaa acgtttcaaa gaactagcta
    52801 tcaagcagat gcaggctggt gagtccgttt ggtttggctc tgatgtgggt caagtttcag
    52861 atcgtcaaaa agggattttg gcaactaata cttacgattt tgaagctagt atggatatca
    52921 atcttagtca agataaggct ggacgtttgg attatagtga aagtttgatg acacatgcta
    52981 tggttttaac aggtgttgac ttagatgaaa caggaaaacc gttaaaatgg aaggttgaaa
    53041 attcatgggg tgagaaggtt ggtgacaaag gttactttgt tgcttcagat gcttggatgg
    53101 atgaatatac ctatcaaatt gttgtccgta aagaattctt aacagctgac gagttagcgg
    53161 cttatgaaaa agagccacaa gtactagctc cttgggatcc aatgggtgct ttagcacaat
    53221 gctaattcct ttctttgcga ctgtatgaaa tctaaaggct ggacaaaacg aacagcaaac
    53281 ggattggact ccgcataaga aagtgaacat gaaaaaaatt attatgctaa gaaaatgaca
    53341 tatgaaaaaa cacgtcttcg gtatatggaa acgtgttttt tcatgtttac tctcttatgg
    53401 cattagttgc tattt
//
LOCUS       DATXQK010000010        49181 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.010, whole genome shotgun sequence.
ACCESSION   DATXQK010000010 DATXQK010000000
VERSION     DATXQK010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 49181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49181
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.010"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(168..467)
                     /locus_tag="VC394_000602"
     CDS             complement(168..467)
                     /locus_tag="VC394_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994094.1"
                     /translation="MFLTWENLFLTNQSRRRLKLLVVERSYNNVGNFLKEYFGGFFEI
                     INFDDLRLTMVDMVSLSSEYDVIVTDMILEQTMDSEILFFNQMAPSVVANRLTDM"
     gene            complement(624..1091)
                     /gene="perR"
                     /locus_tag="VC394_000603"
     CDS             complement(624..1091)
                     /gene="perR"
                     /locus_tag="VC394_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide-responsive transcriptional repressor
                     PerR"
                     /protein_id="HEP5994095.1"
                     /translation="MDIHSHQQALDAYENVLEHLREKHIRITETRKAIISYMIQSTEH
                     PSADKIYRDLQPNFPNMSLATVYNNLKVLVDEGFVSELKISNDLTTYYDFMGHQHVNV
                     VCEICGKIADFMDVDVMDIAKEAHEQTGYKVTRIPVIAYGICPDCQAKDQSDF"
     gene            complement(1143..1598)
                     /locus_tag="VC394_000604"
     CDS             complement(1143..1598)
                     /locus_tag="VC394_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEP5994096.1"
                     /translation="MIYSFQNPSEDILKAYLESAKTIAVVGLSDRKDTAAYGVAKFMQ
                     AMDYRIIPVNPKLAGQLILGEKVYASIKAIPFEVDIVDVFRRSEFLPEVARDFLAGQA
                     KVFWAQLGLENQEVQTILRSAGKEAIVMNRCLKIDYLQLVAKPECITNL"
     gene            complement(1785..4427)
                     /gene="polA"
                     /locus_tag="VC394_000605"
     CDS             complement(1785..4427)
                     /gene="polA"
                     /locus_tag="VC394_000605"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEP5994097.1"
                     /translation="MENKNKLLLIDGSSVAFRAFFALYNQIDRFKNHSGLHTNAIYGF
                     HLMLDHMMKRVQPTHVLVAFDAGKTTFRTEMYADYKAGRAKTPEEFREQFPYIREMLT
                     ALGIAYYELEHYEADDIIGTLDKMAERTEVPFDVTIVSGDKDLIQLTDENTVVEISKK
                     GVAEFEEFTPAYLMEKMGLTPNQFIDLKALMGDKSDNIPGVTKIGEKTGLKLLHEFGS
                     LEGIYEHIDGFKASKMKENLINDRDQAFLSKTLATINTASPITIGLDDIVYNGPDVAS
                     LSQFYDEMDFVQLKKGLASQMPQEPVAAISYQEVTNVSADLFSAEDIFYFETLRDNYH
                     CEAIIGFAWGHGEQIYASTDLSLLATDSFKQVFQKPIATYDFKRSKVLLSHLGIELVA
                     PSYDARLANYLLSTVEDNELSTIARIFTDISLEEDDTVYGKGAKRAVPDKDVLLEHLA
                     RKVKVLLDSKSQMLDKLTAHEQLDLYQNIELPLANVLAKMEIEGIKVNRATLQDMAEQ
                     NKVIIEALTQEIYDMAGQEFNINSPKQLGSILFEKMQLPLEMTKKTKTGYSTAVDVLE
                     RLAPIAPIVAKILDYRQITKLQSTYVIGLQDYILADGKIHTRYVQDLTQTGRLSSVDP
                     NLQNIPIRLEQGRLIRKAFTPSHEDAVLLSSDYSQIELRVLAHISGDEHLIAAFNEGA
                     DIHTSTAMRVFGIDKAADVTANDRRNAKAVNFGIVYGISDFGLSNNLGITRKQAKSYI
                     DTYFERYPGIKAYMENVVREAKDKGYVETLFKRRRELPDINSRNFNVRSFAERTAINS
                     PIQGSAADILKIAMINLDKALQAGGFRAKMLLQVHDEIVLEVPNDELTAIKKLVKDTM
                     EAAVDLAVPLCVDESTGQSWYEAK"
     gene            complement(4558..6285)
                     /locus_tag="VC394_000606"
     CDS             complement(4558..6285)
                     /locus_tag="VC394_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HEP5994098.1"
                     /translation="METILQTKLPVAQLVEQLTEWLTKTFSGLFDIMQVVGSFLMDWM
                     TKTLLFIHPLLFIVLVTAGVFFLAKKKWPLPTFTLLGLLFIYNQGLWKQLMNTFTLVL
                     VASLISVLIGIPLGIWMAKNATVRQIVNPILDFMQTMPAFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLAIRDIPTELIEASDAFGSTGKQKLFKVELPLAKNTIMAGVN
                     QTMMLALSMVVTGSMIGAPGLGREVLSALQHADVGSGFVSGLALVILAIVLDRMTQLF
                     NSKPQEKAKAGKTNKWIGLAALAVFLIAALGRGIMAMTSGMADKGETVNIAYVQWDSE
                     VASTHVIAEVLKNEGYHVTLTPLDNAVMWQTVANGNADFSTSAWLPVTHGQQYQKYKS
                     KLDDLGPNLKGTKLGLAVPKYMTDVNSIEDLSKQADQKITGIEPGAGIMAAAQKTLKE
                     YHNLSSWELVAASTGAMTTSLDQAIKKKDPIVVTAWSPHWMFAKYDLKYLKDPKETFG
                     STENINTIARKGLKKDLPNVYKIIDKFHWTQKDMEAVMLDINKGMSPEAAAKKWVEAN
                     QSKVSSWTK"
     gene            complement(6301..7497)
                     /locus_tag="VC394_000607"
     CDS             complement(6301..7497)
                     /locus_tag="VC394_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEP5994099.1"
                     /translation="METILEVKHLSKIFGKKQKAALEMVKTGKNKSEIFKKTGATVGV
                     YDASFEVKKGEIFVIMGLSGSGKSTLVRMLNRLIEPSAGSILLEGKDISTMSADQLRE
                     VRRHDINMVFQSFALFPHKTILENTEFGLELRGVPKEERQRLAEKALDNSGLLDFKDQ
                     YPNQLSGGMQQRVGLARALANSPKILLMDEAFSALDPLIRREMQDELLDLQDSMKQTI
                     IFISHDLNEALRIGDRIALMKDGQIMQIGTGEEILTNPANDFVREFVEDVDRSKVLTA
                     QNIMIKPLTTTVELDGPQVALNRMHNEEVSMLMATNRRRQLVGSLTADAAIEARKKGL
                     PLSEVIDRDVRTVSKDTIITDILPLIYDSSAPIAVTDDNNRLLGVIIRGRVIEALANI
                     SDEDLN"
     gene            complement(7984..9075)
                     /gene="ulaG"
                     /locus_tag="VC394_000608"
     CDS             complement(7984..9075)
                     /gene="ulaG"
                     /locus_tag="VC394_000608"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEP5994100.1"
                     /translation="MTKVQDITRESWILNTFPEWGTWLNEEIEQEVVPADNFAMWWLG
                     NCGIWIKTPGGANVVMDLWSNRGKATKQVKDMVRGHQMANMAGARKLQPNLRAQPMVI
                     DPFMINELDYYLVSHYHSDHIDINTAAAIINNPKLNHVKFVGPYECGEVWKNWGVPKD
                     RIMILKPGDSFEFKDIKITAVESFDRTCLVTLPIQGADAQDGDLAGLAITDDDMARKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGRDYDIDVVLNNYGENPIGIQDKMTSVDLLR
                     MAENLRAKVVIPVHYDIWSNFMASTDEILELWKMRKERLQYDFHPFIWEVGGKYTYPQ
                     DQNRIEYHHPRGFDDCFLEDSNIQFKALL"
     gene            complement(9328..10974)
                     /locus_tag="VC394_000609"
     CDS             complement(9328..10974)
                     /locus_tag="VC394_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEP5994101.1"
                     /translation="MMILDKKSYDLLSYLLKLETPETVMAISHALNQSRRKVYYQLDK
                     INKALPKGVDQIISYPRLGILLTADQKAACRLLLEEVTDYNYVMKSDERRRLSSIYIA
                     VSTERVTIDKLMQINDVSRNTILNDLTELREELEDKQYKIQLHATKARGYYFGCHPMA
                     LIQYLYKLLVDVYQGGNTSFIDIFNRKLSEIQGLSVYFSKDILTYFHEYLFLSQASLG
                     KTINTQDSQFMLQILPFMLLSYRNMRLDSETKSALKQEFHLIWKRKEYHIAQDLAREL
                     YHNFKLHLDDIEVSMVAMLMLSFRKDQDHHVESQDYDDMRATISHFIDQLESRYQLHF
                     THKQDLLKQLTTHCKALVYRKAYGIFLVNPLTDHIKEKYEELFAMTQSCATILEQDWT
                     ISLTDDDIAYLTIHLGGELRHNNTEQEKTKLVIVSDDGIGIQKLLFKQCQRYLANGQI
                     EAVFTTEQYQSVYDLLAVDMIVATTDTLKTKIPMLIVNPILSDDDIIKLIRFSKQGRL
                     SEHSRFSTELTKAIEAVVKDESDRYALVSKIEKLIHRELL"
     gene            complement(11299..12003)
                     /locus_tag="VC394_000610"
     CDS             complement(11299..12003)
                     /locus_tag="VC394_000610"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEP5994102.1"
                     /translation="MAKNLQEMRERVCAANKSLPQHGLVKFTWGNVSEVCRELGRIVI
                     KPSGVDYDLLTPENMVVTDLDGNVVEGDLNPSSDLPTHVELYKAWPEVGGIVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGPVPCARSLTKAEVDGAYEQETGNVILEEFSKRGL
                     DPMAVPGIVVRNHGPFTWGKTPEQAVYHSVVLEEVAKMNRLTEQINPRVEPAPRYIMD
                     KHYLRKHGPNAYYGQK"
     gene            complement(12005..12868)
                     /locus_tag="VC394_000611"
     CDS             complement(12005..12868)
                     /locus_tag="VC394_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEP5994103.1"
                     /translation="MARPIGIYEKATPKQFTWRERLQFAKDLGFDFVEMSVDESDARL
                     ARLEWTKEERLDLVKAIYETGIRIPTICFSGHRRYPLGSNDPATEAKSLKLMKQCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPETRARFIKNLRQSCDWAEEAQVMLSIEIMDDPFIN
                     SIEKYLAVEKEIDSPYLFVYPDAGNVSAWHNDLWSEFYNGHKSIAALHLKDTYAVTET
                     SKGQFRDVPFGQGCVDWQELFAVLKKTNYNGPFLIEMWSENCDTVEETKAAIKEAQDF
                     LYPLIEKAGLA"
     gene            complement(12873..13535)
                     /locus_tag="VC394_000612"
     CDS             complement(12873..13535)
                     /locus_tag="VC394_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEP5994104.1"
                     /translation="MTHIPNLQVALDHSDLQGAVKAAVAVGHEVDVIEAGTVCLLQVG
                     SELVEVLRSLFPEKIIVADTKCADAGGTVAKNNAKRGADWMTCICCATIPTMEAALKA
                     IKEERGDRGEIQIELYGDWTYEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNKV
                     KKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAEDPAAAARAFKDEIKRI
                     WG"
     gene            complement(13642..14127)
                     /locus_tag="VC394_000613"
     CDS             complement(13642..14127)
                     /locus_tag="VC394_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEP5994105.1"
                     /translation="MNLKQAFIDNNSIRLGLSADTWQEAVQLAVQPLIDSKAVTSAYY
                     DAIIASTEKYGPYYVLMPGMAMPHAEVGLGVNRNAFALITLTKPVTFSDGKEVSVLLT
                     LAATDPSIHTTVAIPQIVALFELDNAIERLVACQSPKEVLEMVEESKNSPYLEGLDLD
                     A"
     gene            complement(14250..14528)
                     /locus_tag="VC394_000614"
     CDS             complement(14250..14528)
                     /locus_tag="VC394_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEP5994106.1"
                     /translation="MVKVLTACGNGMGSSMVIKMKVENALRQLGVTDIQSASCSVGEA
                     KGLASGYDIVVASNHLIHELDGRTKGHLVGLDNLMDDNEIKTKLQEVL"
     gene            complement(14599..16032)
                     /locus_tag="VC394_000615"
     CDS             complement(14599..16032)
                     /locus_tag="VC394_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEP5994107.1"
                     /translation="MEMLLAPLNWFSQNILQNPAFFVGLLVLIGYLLLKKPIYEVFAG
                     FVKATVGYLILNVGAGGLVTTFRPILVALAKKFELKAAVIDPYFGLAAANTKLEEMGF
                     ISVATTALLIGFGVNILLVALRKVTKVRTLFITGHIMVQQAATISVFVLLLIPQFQNA
                     FGAWAVGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAPIFGKKE
                     ENLDNLKLPTFLNIFHDTVVASATLMLVFFGAILAVLGPDIMSNVELIGPGAFNPAKQ
                     AFFMYILQTSLTFSVYLFILMQGVRMFVSELTNAFQGISSKLLPGSFPAVDVAASYGF
                     GSSNAVLSGFAFGLIGQLITIALLVIFKNPILIITGFVPVFFDNAAIAVYADKRGGWK
                     AAVALSFISGILQVALGAVAVGLLGLTGGYHGNIDLVLPWLPFGYLFKFLGIAGYVLV
                     CIFLLAIPQLQFAKAKDKEAYYRGEAQ"
     gene            complement(16339..18840)
                     /locus_tag="VC394_000616"
     CDS             complement(16339..18840)
                     /locus_tag="VC394_000616"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEP5994108.1"
                     /translation="MTFYDHTAIEPKWQAFWADNHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRFKRAQGHNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLEDLEEVDWPESIKD
                     MQRNWIGKSTGANVTFKVKDTDKDFTVFTTRPDTLFGATYAVLAPEHALVDAITTADQ
                     AEAVADYKRQASLKSDLARTDLAKEKTGVWTGSYAINPVNGNEMPVWIADYVLASYGT
                     GAIMAVPAHDERDWEFAKQFNLDIISVLEGGNVEEAAFTEDGLHINSGFLDGLDKASA
                     IAKMVEWLEAEGVGNEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPESELPLV
                     LPVTKDIRPSGTGESPLANVTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEELLKQWLPVDIYVGGAEHAVLHLLYARFWHKVLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDSRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITTKEITEENSGALD
                     KVYNETVKAVTEQVDQMKFNTAIAQLMVFVNAANKEDKLFSDYAKGFVQLIAPFAPHL
                     GEELWQALTASGESISYVPWPSYDKSKLVENDVEIVVQIKGKVKAKLVVAKDLSREEL
                     QEVALANEKVQAEITGKDIIKVIAVPNKLVNIVIK"
     gene            complement(19051..20190)
                     /locus_tag="VC394_000617"
     CDS             complement(19051..20190)
                     /locus_tag="VC394_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEP5994109.1"
                     /translation="MRENTTLLHGYTVIDEFTGAASVPIYQTSTFHNSELYCPSQKHL
                     YTRFSNPTTEALEDGLACLEKATYAVAYASGMAAISTVLMLLKAGDHVIFPLEVYGGT
                     CQFATAILPNYQIETSFVDMADLATVKASIRPNTRMIYLETPSNPLLKICDISELVQL
                     AKAYGVLTVADNTFMTSLYQEPLAMGVDIVVESVTKFINGHSDVVAGLAATNNEAIYN
                     QLKLFQKNFGAIVGVEDAWLILRGMKTMGIRMEQAVKNAQQLANYLAKHPKVLKVHYP
                     GLDSHPNHDTHLQQAKNGGAVLSFELASKEELMIFTHRIQLPILAVSLGGVESILSHP
                     ATMSHACLSSQARLEQGVVDGLLRLSCGVENIEDLLADFEQALAF"
     gene            20674..20952
                     /locus_tag="VC394_000618"
     CDS             20674..20952
                     /locus_tag="VC394_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994110.1"
                     /translation="MKNKLFLVALATVTVLGPSLATPHHRTVHATEQTVTQRTATETC
                     TLNGKICYGYENPDEDYTDSMVQSKSQDQGFFSWLWTQITTWFSSLMS"
     gene            21095..21325
                     /locus_tag="VC394_000619"
     CDS             21095..21325
                     /locus_tag="VC394_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994111.1"
                     /translation="MKKKLSLFMIATAAVVGLSLATPENQTVSANAQTPAVQNCENGC
                     CDRPFFGYCGPFSWLYYNTRYFLGYSDTATNQ"
     gene            complement(21609..21986)
                     /locus_tag="VC394_000620"
     CDS             complement(21609..21986)
                     /locus_tag="VC394_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994112.1"
                     /translation="MKQQSYKPLHFVYLLMALLTAMLLIARPVMADEEDKDPWRLGYN
                     QDLTKGKEDGKVAEWTDLTPPDPEVPKSPKDIDDSNHEHYRDGYKDGYKEGYNEGFGG
                     SYPLLLPVKFIWGLILHWWEKLI"
     gene            complement(22237..23952)
                     /gene="slo"
                     /locus_tag="VC394_000621"
     CDS             complement(22237..23952)
                     /gene="slo"
                     /locus_tag="VC394_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_095889392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin streptolysin O"
                     /protein_id="HEP5994113.1"
                     /translation="MSNKKIFKKYSRVAGLLTAALIVGNLVTANADSNKQNTANTETT
                     TTNEQPKPESSELTTEKAGQKMDDMLNSNDMIKLAPKEMPLESAEKEEKKSEDNKKSE
                     EDHTEEINDKIYSLNYNELEVLAKNGETIENFVPKEGVKKADKFIVIERKKKNINTTP
                     VDISIIDSVTDRTYPAALQLANKGFTENKPDAVVTKRNPQKIHIDLPGMGDKATVEVN
                     DPTYANVSTAIDNLVNQWHDNYSGGNTLPARTQYTESMVYSKSQIEAALNVNSKILDG
                     TLGIDFKSISKGEKKVMIAAYKQIFYTVSANLPNNPADVFDKSVTFKELQRKGVSNEA
                     PPLFVSNVAYGRTVFVKLETSSKSNDVEAAFSAALKGTDVKTNGKYSDILENSSFTAV
                     VLGGDAAEHNKVVTKDFDVIRNVIKDNATFSRKNPAYPISYTSVFLKNNKIAGVNNRS
                     EYVETTSTEYTSGKINLSHQGAYVAQYEILWDEINYDDKGKEVITKRRWDNNWYSKTS
                     PFSTVIPLGANSRNIRIMARECTGLAWEWWRKVIDERDVKLSKEINVNISGSTLSPYG
                     SITYK"
     gene            complement(23976..24461)
                     /locus_tag="VC394_000622"
     CDS             complement(23976..24461)
                     /locus_tag="VC394_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD glycohydrolase inhibitor"
                     /protein_id="HEP5994114.1"
                     /translation="MYKVPKGLEHYQKMFQKEVTVNDLKKYLIGSDKEYRITKRDSYM
                     GDISDPEVILEYGIYPAFIKGYTQLKANIEEALLKMSNSGQALDIYQAVQTLNAENML
                     LNYYESLPFYLNRQSILANITKALKDAHIREAMAHYKLGEFAHYQDTMLDMVERTIET
                     F"
     gene            complement(24466..25809)
                     /gene="nga"
                     /locus_tag="VC394_000623"
     CDS             complement(24466..25809)
                     /gene="nga"
                     /locus_tag="VC394_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotine adenine dinucleotide glycohydrolase"
                     /protein_id="HEP5994115.1"
                     /translation="MRNKKVTLAHIVAKTSVAIALAGAMGSSLLANNTTYAVSGKEGK
                     KSDVKYEMTKVMEANATSSKEDKHVMHTLDGSMSTVWEENSPGGGIGEVLSYKFASPM
                     HIGRILIVNGDTSSKENYYKKNRIAKADVKYYNGNKLVLFQKIELGDTYTKKPHHIEI
                     DKKLDVDRIDIEVTEVHQGQNKDILALSEVTFGNMERDIFEKKFKEIKDKWVTDKQAD
                     EFIETADKYADKAVQMSAVASRAEYYRMYVSRKYHYKKEFVEKLKQVYKESGASHVTS
                     KKDLMLAFDDAKRKSTIGRQENGLFVTSFAEDMALLFTDQGKLKSADQIENIKGVDSG
                     KYSDGVYQYEYDSELTKNIDKLGYIRTASGDTPGANSLNIPGCQTWSGKHIENSESEL
                     IFPSISVKDLKSKAVLAEIDAKGYFEIIDPTIIAPNGDHKKVTGRFKIKKMQDRK"
     gene            complement(26177..26716)
                     /gene="nusG"
                     /locus_tag="VC394_000624"
     CDS             complement(26177..26716)
                     /gene="nusG"
                     /locus_tag="VC394_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003100341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEP5994116.1"
                     /translation="MLDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEI
                     PTQTVNVEKNGQTKEIEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKP
                     TPLLEEEIRAILLSMGQTIDVFDTNIKEGDVVQIIDGAFMGQEGRVVEIENNKVKLML
                     NMFGSETVAEVELYQIAEL"
     gene            complement(26889..27932)
                     /locus_tag="VC394_000625"
     CDS             complement(26889..27932)
                     /locus_tag="VC394_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEP5994117.1"
                     /translation="MNKKVMSLGLVSTALFTLGGCTNNSAKQTTDNSLKIAMITNQTG
                     IDDKSFNQSAWEGLQAWGKENKLEKGKGYDYFQSANESEFTTNLESAVTNGYNLVFGI
                     GFPLHDAVEKVAANNPDNHFAIVDDVIKGQKNVASITFSDHEAAYLAGVAAAKTTKTK
                     QVGFVGGMEGDVVKRFEKGFEAGVKSVDDTIKVRVAYAGSFADAAKGKTIAAAQYAEG
                     ADVIYHAAGGTGAGVFSEAKSINEKRKEEDKVWVIGVDRDQSEDGKYTTKDGKSANFV
                     LTSSIKEVGKALVKVAVKTSEGQFPGGQITTFGLKEGGVSLTTDALTQDTKKAIEAAK
                     KAIIEGTITVPEN"
     gene            complement(28259..29551)
                     /locus_tag="VC394_000626"
     CDS             complement(28259..29551)
                     /locus_tag="VC394_000626"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEP5994118.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSADAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPQKISVIGNGVVVNPKSLVKELAYLHDEGVTTDNLRISDRA
                     HVILPYHIQLDQLQEDAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFAERL
                     RINLAEKNRLFEKMYDSTPLDFDAIFEEYYAYGQEIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGERIDHYPASLEQLKRCKPIYEELPGWQEDITGVRSLDELPEN
                     ARNYVRRVGELVGVRISTFSVGPGREQTNILESVWASI"
     gene            29989..30867
                     /locus_tag="VC394_000627"
     CDS             29989..30867
                     /locus_tag="VC394_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994119.1"
                     /translation="MIRKYDRTSTKKKSLNWIWLIIAFFMISSFIGGSSFTESLLDIL
                     PAIAIGGTGYAIFRVRSHQKRLAKAKVAKQLEDLKAKIQLADRKVRLLDTYLADHDDF
                     QYNVLAQQLLPQLSDIKAKAITLKDQLDPKIYRRITKKANDVESDITLQLETLQIATT
                     LNPQPLKTPSPNLINKAPELKPYYDNIQTDHQAILAKIQGADNQEELLALHEANMRRF
                     EDILTGYLKIKEEPKNYYNAAARLEQAKQAIQQFDENLDETLRRLNESDLKDFDISLR
                     IMQGATQRRTTHHQKD"
     gene            30880..32142
                     /locus_tag="VC394_000628"
     CDS             30880..32142
                     /locus_tag="VC394_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEP5994120.1"
                     /translation="MADFNFDIDQIADNAVVKTDKTTDIISDLPTDTNGQISFFEKLS
                     ADQQTAITARAPALVDTFLADQNALLDFGQSAVEGVNATVNHILAEQKKLQIPQVDDL
                     LKSTNRELNGFIAKYKDATPVDLDKKPNFLQKLFKQSRDTLQEFYFDSQNIEQKMDSM
                     AAAVVKQEDTLARNIVSAELLIEDNTKSIEHLVGVIAFIEASQKEASQRAAALQKDLK
                     TKDSATPDYQIKADLLARTTEVINTLEQQHTEYLSRLYVAWATTPQMRNLVKVSSDMR
                     QKLGMLRRNTIPTMKLSIAQLGMMQQSVKSGMTADAIINANNAALQMLAETSKEAIPA
                     LEQSAQNPTLSMKSVTSLAESLVAQNNGIIAAIDHGRKERAQLESAIIRSAETINDSV
                     KLRDQNIVQALLSEGKETQKTIDKTTRA"
     gene            complement(32262..32888)
                     /locus_tag="VC394_000629"
     CDS             complement(32262..32888)
                     /locus_tag="VC394_000629"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEP5994121.1"
                     /translation="MARLNVKPTRMELSNLKNRLKTATRGHKLLKDKRDELMRRFVDL
                     IRENNELRQTIEKELAANMKEFVLAKASENSLMVEELFAVPVHEVTLWIDIENIMSVN
                     VPKFHVQSNTAREQEQGEFAYSYLSSNSEMDNTIQKTKELLEKLLRLAEVEKTCQLMA
                     DDIEKTRRRVNGLEYSIIPQLKETIHYIELKLEEAERASLVRIMKITS"
     gene            complement(32933..34348)
                     /locus_tag="VC394_000630"
     CDS             complement(32933..34348)
                     /locus_tag="VC394_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEP5994122.1"
                     /translation="MSVLKEYRTVSEVVGPLMIVDQVAGVHYNELVDITLHNGERRKG
                     QVLEVQGDKAMVQLFEGSTGINLAKTKVRFTGHPLELAVSEDMVGRIFDGMGQPIDGG
                     PELIPEKYLDIDGQAINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHN
                     ELAAQIARQATVLNSDDNFAVVFAAMGITFEEAEFFMNDLRETGAIDRSVLFINLAND
                     PAIERIATPRIALTTAEYLAYEKGMHVLVIMTDMTNYCEALREVSAARREVPGRRGYP
                     GYLYTNLSTLYERAGRLIGKKGSVTQIPILTMPEDDITHPIPDLTGYITEGQIILSQE
                     LYKNGFRPPINVLPSLSRLKDKGSGEGKTRQDHAATMNQLFAAYAQGKQAKELAVVLG
                     ESALSETDKLYVAFTNRFEEEYINQGFYTNRSIEESLDLGWELLSILPRTELKRIKDD
                     MLDRYLPKADTTMTKVFVAND"
     gene            complement(34349..36124)
                     /locus_tag="VC394_000631"
     CDS             complement(34349..36124)
                     /locus_tag="VC394_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEP5994123.1"
                     /translation="MNQGKIITVSGPLVVASGMQEANIQDICRVGHLGLVGEIIEMRR
                     DQASIQVYEETSGIGPGEPVVTTGCPLSVELGPGLISEMFDGIQRPLDRFQKATDSDF
                     LIRGVAIPSLDRKAKWAFIPKLSVGQEVVAGDILGTVQETAVIEHRIMVPYKVSGTLV
                     AIHAGDFTVTDTVYEIKQEDGSIYQGSLMQTWPVRQSRPVAQKFIPVEPLVTGQRVID
                     TFFPVTKGGAAAVPGPFGAGKTVVQHQIAKFANVDIVIYVGCGERGNEMTDVLNEFPE
                     LIDPNTGQSIMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYSVAIMADSTSR
                     WAEALREMSGRLQEMPGDEGYPAYLGSRIAEYYERAGRVRTLGSQEREGTITAIGAVS
                     PPGGDISEPVTQNTLRIVKVFWGLDAPLAQRRHFPAINWLTSYSLYQDDVGSYIDRKQ
                     QSNWSNKVTRAMAILQREASLEEIVRLVGLDSLSEQDRLTMAVARQIREDYLQQNAFD
                     SVDTFTSFPKQEAMLTNILTFNEEASKALSLGAYFNEIMEGTAQVRDRIARSKFIPEE
                     NLEQIKGLTQKVTKEIHHVLAKGGI"
     gene            complement(36325..36645)
                     /locus_tag="VC394_000632"
     CDS             complement(36325..36645)
                     /locus_tag="VC394_000632"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEP5994124.1"
                     /translation="MTKTLYKVGVIGNRDVILPFQMIGFQTFPVIKPQDAINQLRQLA
                     MEDFGIIYITEDIAAAIPEALTHYDNQVLPAVIPLPTHQGAQGIGLSRIQAMVEKAVG
                     QNIL"
     gene            complement(36642..37640)
                     /locus_tag="VC394_000633"
     CDS             complement(36642..37640)
                     /locus_tag="VC394_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEP5994125.1"
                     /translation="MPTFLELNTTISVKEKELLTKEQFDKLLQAPNTTTLARLLHQSV
                     YHLTVDDLNDLDRLESILMAELTKTYRWAFAETPQPDIVQLFTLRYTYHNVKVLLKAK
                     ASQADLSHLLLPIGDKPLVALEHLIRTMTSDEFPKEVVTEIQSIWAEYQDYQDIRVLE
                     IGTDLAYFKALKQIAQRLEDPVFQQAVLIVIDLYNLITVRRAKSQNKPISFMMQLLSD
                     EASRPSKTFITLEDDKDLMTWFENVTPDSYMTALKPYSEKLRQGTLQTTELEYLVDEC
                     LYHLFAKAKYQVDGPYVLARFLLAKSFEVKNLRLLAAALANDLPKERVIERMRPIA"
     gene            complement(37656..38240)
                     /locus_tag="VC394_000634"
     CDS             complement(37656..38240)
                     /locus_tag="VC394_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HEP5994126.1"
                     /translation="MNDITQLRQNVLEKAHQEGQQCLKIATDSLDTDFKERQQQGLHD
                     LKAKRQKELKAFEQQFQVAQQQLKNQERQALLALKQDSIKELFEASLEKMTNFSKEEE
                     LAFLKQVLSKYPEQPLQVTFGEKTGQKFSSYDCAELRLAFPQLSYNQELIPQEAGFLV
                     SLDQVDDNYLYRYLLESVLKEESSRIIDMLFSEI"
     gene            complement(38309..38788)
                     /locus_tag="VC394_000635"
     CDS             complement(38309..38788)
                     /locus_tag="VC394_000635"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEP5994127.1"
                     /translation="MEHLAHYFTAHGGVFFAALGIVLAVALSGMGSAYGVGKGGQAAA
                     ALLKEEPEKFTSALILQLLPGSQGIYGFAIGILIWMKLTPELSVNQGLAYFLVSLPIA
                     IVGYFSAKHQGNVSVAGMQILAKRPKDFMKGVILAAMVETYAILAFVVSFILLNKIG"
     gene            complement(38790..40790)
                     /locus_tag="VC394_000636"
     CDS             complement(38790..40790)
                     /locus_tag="VC394_000636"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEP5994128.1"
                     /translation="MAISQMKKLAMVFEKDYLDLVLKTLQQSQLVEVRDMKQLKHWQD
                     AFNKGNVKLPQIVQYDLTHQKPLLDDEALQYLLQSQQELENGLASLSAFLPPIGKLTA
                     LRQKTPSLSFKQFEERHRQQAAQTALKMMSQKIERLEQLQNKIDQLTEYCQELEKWRS
                     LTVLPQDLAQFHFLSARVGTIPSTANDHFYHQLKKHKGLFIEEVYHTEFEYGLVLFWQ
                     AQDTIHLQKYQFKPLLYKEQLLPSEQLRINKELLTNWLAEKDSLLKELRQSQKILAQL
                     QVEIDYVLSQYQRQQTKKQLLGTRHLIALEGWIEADSVNQLKGLMTKTLGDMFYLDSY
                     DVTPDDWEDVPIKLRNHRYIAPFELVTEMYALPKYQEKDPTPFLAPLYLTFFGMMVAD
                     LGYGLLLYAVTLAALVFFNLQKTSKRLVTFFNILAISVALWGLIYGSFFGFDLPVALL
                     STKTDVITILVVSLLFGFVTLIFGLLLGAWQQVRMKAYATAYTSSLAWTFILLGLLLF
                     ILGKNVSGLAYLSVIGKWLALGNAFGILVVSLLKSKSLLGLGSGLYNLYGISSYLSDL
                     VSFTRLMALGLSGASIGAAFNMIVGIFPPVTRFTVGIFIFILLHAINIFLSMLSGYVH
                     GARLIFVEFFGKFYEGGGKAFNPLKLADNYVNVNEETDLEDN"
     gene            complement(40780..41100)
                     /locus_tag="VC394_000637"
     CDS             complement(40780..41100)
                     /locus_tag="VC394_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994129.1"
                     /translation="MNTLLSLMNSIEQEARTIYDSYDAKKKALADKKEEELKQLFLEY
                     EQETQLLLEAKREVFFEANRQEQKASKKRLATQQLALHAILEEHKLTVVRELVDKVVE
                     TYGH"
     gene            complement(41554..42573)
                     /locus_tag="VC394_000638"
     CDS             complement(41554..42573)
                     /locus_tag="VC394_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HEP5994130.1"
                     /translation="MDIIIGTSLLILVLAIFSLFNYKAPHGAKAMGALASAACASFLV
                     EAFQDSFFGKVLGFQFLSEVGGANGSLSGVAAAILVAIAIGVSPGYAVLIGLSVSGTG
                     IIPGFVAGYVVSFLIKWMEKNIPGGLDLISIIIVGAPLTRFLAQLITPVINSTLLTIG
                     DILTSSANSNPIIMGMILGGTIVVVATAPLSSMALTAMLGLTGIPMAIGALSVFGSSF
                     MNGVLFYRLKLGERKDNIAFAIEPLTQADVTSANPIPIYVTNFVGGAACGVLIALMKL
                     VNDTPGTATPIAGFAVMFAYNPVAKVLITALGCIIISLIVGYIGGSVFKNYRLVTKQE
                     LQARN"
     gene            complement(42610..42975)
                     /locus_tag="VC394_000639"
     CDS             complement(42610..42975)
                     /locus_tag="VC394_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEP5994131.1"
                     /translation="MKSYPEPMGPYSTYTIEGHFLYTAGQLPLNPVTGQLSDGFEAQC
                     RQVFVNLQSILAEQKLDLNHIYKLNVYLTDVTNVEILNHVMTDLFEEPYPVRTAVQVS
                     ALPLQALIEVEAVARVKQL"
     gene            43149..43829
                     /locus_tag="VC394_000640"
     CDS             43149..43829
                     /locus_tag="VC394_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEP5994132.1"
                     /translation="MDKETLNYWKTVITFLHDVLGDNYEIILHVIDKNDIYIGELVNS
                     HISGRSKQSPLTTFALDLITNKVYKEKDFVTNYKAIVSPQHKEVRGSTFFIKDKKGNL
                     EGMLCINLDISAYQGVARDLLKLVNLNLEHFIPTAKEPKTVTPQPEEAVEILTSNIQD
                     IIGQIIDPSLLRHNVHLSQDVKIDIVAKLYEKGVFQLKGAVSKVADILCISEPSVYRY
                     LKKIEADQ"
     gene            complement(43950..44597)
                     /locus_tag="VC394_000641"
     CDS             complement(43950..44597)
                     /locus_tag="VC394_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEP5994133.1"
                     /translation="MAVALSKEEIQNRIAKRVAKELEDGTLVNLGIGLPTKVANFVPE
                     EMTVYFQSENGFIGLGPKSDDPNSTIVNAGGQPVTVYPGAAFFNSADSFGIIRGGHVD
                     LTVLGALEIAENGDIANYLIPGKMVPGMGGAMDLLVGAKKVIVAMEHTNKGKHKLLKE
                     CTLPLTAKGVVDLIITEMGVFKVTPDGIQVIEISEGFTFDEVQAATGVPLSIAEG"
     gene            complement(44600..45259)
                     /locus_tag="VC394_000642"
     CDS             complement(44600..45259)
                     /locus_tag="VC394_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HEP5994134.1"
                     /translation="MKENKRIAIAEAISHIKDGDTIMVGGFMANGTPEALIDALVDKG
                     TKDLTLICNDAGFVDRGVGKMVANHQFKTIYATHIGLNKEAGRQMTAGETTIELIPQG
                     TFAEKIRIGAYGIGGFYTPTGVGTLVAEGKETKTIKGKTYLLEYPFEADVTLIFANKA
                     DEMGNLQYSGSENNFNQLMAACAKTTIVQVREIVPVGTIQPECVHTPHIFVDYIVKEG
                     V"
     gene            complement(45271..46455)
                     /locus_tag="VC394_000643"
     CDS             complement(45271..46455)
                     /locus_tag="VC394_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEP5994135.1"
                     /translation="MTKEVVITSAYRTPIGNFGGVFKSLSAVDLGVTVVTKILADTGL
                     KSDAIDEVIFGNVLHAGLGQNVARQVALNAGLSYDTPAFTIDMVCGSGLKAVELGAQK
                     IQTGNADIVLVGGTENMSQAPYVLQGQRWGSRMGDSKVVDTMLKDGLSDAFAGYHMGI
                     TAENIVQQYGLTREEQDAFAADSQRKAQLAIEKGRFKEEIAPVTIPQRKGESLLVDQD
                     EYPKFGTTVDKLAKLRPAFIKDEGTVTAGNASGINDGAAAILLMSKEKAEELGLPILA
                     KITSYASAGVDPSIMGCGPIPATKKALAKAQLTIDDIDLIEANEAFAAQALAVSRDLG
                     FDNEKVNVNGGAIALGHPIGASGARILVTLLAEMAKRDVRHGLATLCIGGGQGQSIIV
                     TR"
     gene            46576..47487
                     /locus_tag="VC394_000644"
     CDS             46576..47487
                     /locus_tag="VC394_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEP5994136.1"
                     /translation="MNISSLAYFIEIVNCDFNLTKAAQKSHISQPALSSYIKTIEKEE
                     GVVLFQRQKGRLVGLTSVGERFFENAKVVIASHEQLMADLRSESKKYKGEISIGIPQL
                     ISGIVFSELIPKLIEENPEAKFHIVETGAYELQKKLFLKEVDFAILLTPTTLDATIAD
                     IDILLRDQLCLYMSHKTFQETFDSNPNISIKALHRQNLVTFDKTFMINHQLTTLFEEA
                     NSLPVVKCYSASWDFLLNCTRYSSYLTILPRPITHFAHMDGLVEVQLTEHPKWEVVLA
                     SLKHNKTSHLKHYIKHTILDYFMNQRF"
     gene            complement(47557..48963)
                     /locus_tag="VC394_000645"
     CDS             complement(47557..48963)
                     /locus_tag="VC394_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain fatty acid transporter"
                     /protein_id="HEP5994137.1"
                     /translation="MNNIKTKKRFMDRYIDGFMKWMPESLFICFILTFLVVTMSVLMT
                     DSPFIGTEKTGGIIYGWVNGFWGLLSFAMQMTILLATGNAVASSPPAHKMFKSLAKLP
                     QTRTQIFIFSIVVGSIFGFLHWGLGMMVAIVFGKELLVQARQKGIKVHTPLFVATLFF
                     TFLPATSGLSGAAVLYSATPDYLRNSVADAYKQVVPESVPLTESVLNLPFISLLVVCM
                     LVPLCFALLAHPKDETKIMELDDEIYHHSLDTASHVVIARNTPAEKMNASRLVMYLVG
                     GAIVSYSLYHFSVVGLSGLDLNSFNFLFLGLGLLLCGQQGPEYYGSLFKDGVMSSWGL
                     VLQFPFYAGIFGIIQSTGLGLEISHFFVAISNGTTWPVFAYLYSALLNIAVPSGGSKF
                     VIEAPYIVPATIEVGNDLGKILQAYQLGDATTNLIVPFWALSYLSNFKLKFNQIVAYT
                     IPCVLVVTGIAIIYLFVF"
BASE COUNT        14714 a        10654 c         8945 g        14868 t
ORIGIN      
        1 tctttttcga gctttttgtt ttttaatttt tcaagttgaa taattaaacc ttgcttttct
       61 ttttcaaggt aatagactct actccttgta agtctaactt attcttaatc ttatttatcg
      121 ctcatttttt aaattccgat gctctcacag atattatcat tgtttagtta catgtctgtc
      181 aatctattcg ccaccacact aggggccatc tgattgaaaa ataaaatttc ggagtccatt
      241 gtttgctcaa gaatcatatc tgtcacaatt acgtcatact ctgaagacag tgacaccata
      301 tcaaccatgg tcagcctcaa gtcatcaaag ttgataatct caaaaaatcc tccaaaatac
      361 tcttttaaga agttgccaac attgttataa cttctctcaa ccactaatag tttcaagcga
      421 cgacgcgatt gatttgtaag aaagagattt tcccaggtga ggaacaaaca aatagaggag
      481 atgattgatg gttctttcct ttaattcctt atgatcccca gcaaataact ctttaacata
      541 agcaacaaag gtctggtatt gaactggggg tttttgattt gtaaaggcac tatatttata
      601 atgcctttat ttaagggtta actctaaaaa tcagattggt cttttgcttg gcaatccgga
      661 caaatcccgt aagcaataac tgggatacgc gtgaccttat aacctgtttg ctcatgggct
      721 tctttggcaa tatccataac atcaacatcc ataaagtcag caatcttccc acatatttca
      781 caaaccacat tgacgtgttg atggcccata aagtcatagt aggtggttaa atcattgctg
      841 attttcaact ctgagacaaa accttcatca actaaaactt tcaaattatt atagactgtg
      901 gcaaggctca tgttaggaaa gttaggctgt aagtcacggt aaatcttatc ggcactaggg
      961 tgttctgtgg actgaatcat gtaagaaata atggctttac gtgtctctgt aattcgaata
     1021 tgtttttccc gtagatgctc taggacattt tcataggcat ctagggcttg ctgatgtgaa
     1081 tgaatgtcca taagctgcta ctcctatcct tttttccttt attatcacgc acgtctctag
     1141 tttcaaagat ttgtgatgca ttctggttta gcgactaatt gcaaatagtc tatttttagg
     1201 cagcgattca tcacaatagc ctctttgcct gccgaccgta aaatggtttg tacttcttgg
     1261 ttttcaaggc ctaattgcgc ccaaaagact ttggcctgac ctgctaaaaa gtctctggca
     1321 acttctggta aaaactcact tctgcgaaag acatctacaa tgtcaacctc aaagggaatt
     1381 gccttgatac tggcatagac cttctctccc aaaataagct gacctgctaa tttgggattg
     1441 actggaatga tccgataatc catagcctgc ataaatttgg cgacaccata agctgctgtg
     1501 tctttgcggt cagaaagacc aacaaccgca atggttttgg ctgactccaa gtaagctttt
     1561 aggatatcct ctgagggatt ttgaaacgaa taaatcactt gttcccctat ctatttaata
     1621 acctctacag ctgttcaata tagcaggata actcctaaag catggcttca atcactttta
     1681 accttatccg tcaaaaagct caaagcagct tcatgacttc tcctacaatt ttttagttta
     1741 ttataacata agttgtcaaa atgagaaata acaagtaaga aaacttattt ggcttcgtac
     1801 cagctctgcc cagtactttc atcgacacat aaaggaacag ctaaatcaac agcagcttcc
     1861 atggtgtctt tgacaagttt ttttatagca gtcaactcat catttggcac ttccaaaacg
     1921 atttcatcat gcacttgaag cagcatttta gctctaaaac cacctgcttg caaggcctta
     1981 tccagattaa tcatagcgat tttgagaata tcagctgcgc ttccttgaat gggcgagttg
     2041 atggctgtac gctccgcaaa agagcgaacg ttgaaattac gagagttgat atccggtaat
     2101 tcacgacgac gcttaaagag ggtctctaca tagcccttgt ctttagcctc acgcaccaca
     2161 ttttccatat aggctttaat cccaggatag cgttcaaaat aggtatcaat gtaagacttg
     2221 gcctgtttac gggtgatacc aaggttattt gacaaaccaa aatcagaaat cccatacaca
     2281 attccaaagt taacagcctt ggcattacgg cggtcattgg cagtaacatc tgctgccttg
     2341 tcaatcccaa acacacgcat agccgttgag gtatgaatgt ctgccccttc attaaaggca
     2401 gcaatcaaat gctcatcccc tgaaatatgt gccagtaccc gcaattcaat ctgtgagtaa
     2461 tctgaactca acaagacggc atcttcatga gatggggtaa aggccttacg aatcaaacgc
     2521 ccctgttcca aacgaatggg aatgttttga agattagggt ccacactaga caaacgccct
     2581 gtctgggtga gatcctgcac atagcgggta tgaatcttgc catcagccag aatataatcc
     2641 tgtaaaccaa tcacataggt ggattgcaat ttagtaatct ggcggtaatc caaaatttta
     2701 gccacaattg gagcaatcgg tgccaaacgt tccaaaacat caacagctgt agaataacct
     2761 gttttggtct tcttagtcat ctcaagaggt agttgcatct tctcaaacaa gatactgccc
     2821 agctgtttag gcgaattaat gttaaattct tggccagcca tatcataaat ctcttgggtg
     2881 agcgcttcaa taatgacctt gttttgctct gccatatctt gcaaagtcgc cctattaacc
     2941 ttgatccctt caatttccat tttagcaagg acattagcca aaggcaattc aatattctga
     3001 tagaggtcta gttgctcatg ggcagtaagc ttgtctagca tttgagactt gctatctaat
     3061 aatactttga ccttacgagc taagtgttct agtaaaacgt ctttatcagg aacagcccgt
     3121 ttagcacctt taccatagac tgtatcgtct tcttccaaag agatgtcagt aaaaatgcgg
     3181 gcaatggtgc taagctcatt gtcttctaca gttgacagaa ggtaattagc taatctagca
     3241 tcatagctag gtgctaccaa ctcaattcct aagtggctca acaagacctt gctacgttta
     3301 aaatcataag tggcaatggg tttttggaag acctgcttaa aactatccgt cgctaatagg
     3361 ctaaggtcag tggatgcgta aatctgctca ccatgtcccc aagcaaaacc aatgatggcc
     3421 tcacaatggt aattatcccg aagcgtttca aagtagaaga tgtcttctgc gctaaacaaa
     3481 tcagcagaca cattggtcac ttcttgatag gaaatggcag ctactggctc ttgaggcatc
     3541 tggctagcaa gacctttctt gagttgcaca aaatccatct catcgtaaaa ttgagagagg
     3601 ctagcaacat ctggaccatt gtagacaata tcatctaagc caattgtgat aggagatgct
     3661 gtattgatag tggcgagcgt tttagatagg aaagcttggt cacggtcatt gatcaagttt
     3721 tctttcattt ttgaggcctt gaatccatca atatgctcgt aaatgccttc caaactacca
     3781 aattcatgaa ggagcttgag cccagttttt tcaccgattt tggtcactcc tggaatatta
     3841 tccgatttat ctcccatcaa ggctttgagg tcaataaact ggtttggcgt caagcccatt
     3901 ttttccatga ggtaagccgg tgtgaattct tcaaattcgg caaccccttt tttagaaatt
     3961 tcaaccacgg tattttcgtc agttaactgg atcaaatctt tatcaccact gacaatggtc
     4021 acatcaaaag gcacttctgt gcgctccgcc attttatcaa gcgtcccgat aatatcgtct
     4081 gcttcgtagt gttccaactc ataataagca atcccaaggg ctgtcaacat ctcacgaata
     4141 taaggaaact gctcacgaaa ttcctctggt gtcttggcgc gacccgcctt ataatcggca
     4201 tacatttctg ttcggaaggt ggttttgccc gcatcaaaag ccaccagcac gtgagtaggc
     4261 tggactcttt tcatcatgtg gtctagcatc aaatgaaagc cataaatggc attggtatga
     4321 agaccgctat gatttttaaa gcggtcaatt tgattgtaga gggcaaaaaa ggcacgaaag
     4381 gcaacagatg agccatcaat taatagtaat ttattcttgt tttccatagc cttattataa
     4441 cacagaggga cgggcttttc tcctttaagc cattggtttt gctgataagt aaatagttaa
     4501 cctaaaaagc ctaggaaaat tcctagactt ctctatttgc tatttagaca aaagcactta
     4561 ttttgtccaa cttgatactt tagattggtt tgcctctacc cattttttgg cggcagcttc
     4621 tgggctcatg cctttgttga tgtcaagcat gactgcttcc atatcttttt gggtccagtg
     4681 gaacttatca atgattttgt agacatttgg aagatctttt ttcaagcctt tacgagcaat
     4741 ggtattgatg ttctcagtcg agccaaaggt ttctttagga tctttaaggt acttcaagtc
     4801 atacttggca aacatccagt gtggtgacca agctgtcacg acaataggat cttttttctt
     4861 gatcgcttga tccaaggaag tcgtcatggc tcctgttgat gccgccacta actcccaact
     4921 agatagatta tgatactctt ttagagtctt ttgggctgct gccatgatac ctgcacctgg
     4981 ttcgatccct gtgatttttt gatcggcttg tttgcttaag tcctcaatac tattaacatc
     5041 tgtcatgtat tttggaacag ctaaaccaag tttggtccct tttaggttag gtcctaaatc
     5101 atctaatttt gacttgtatt tctgatattg ttggccatgg gtgactggca accaagcact
     5161 agttgaaaaa tcagcgttcc catttgcaac ggtttgccac ataacggcat tgtcaagcgg
     5221 agtcaaggtc acatggtaac cttcgttttt caaaacttct gcaatcacat gagttgaggc
     5281 aacctcagaa tcccactgca cgtaagcgat gttaacagtt tcacccttat ctgccatacc
     5341 agaagtcata gccataatac cgcgtcctaa agcagctatc aaaaagacag caagggctgc
     5401 tagaccaatc catttattgg ttttgccagc tttggctttt tcttgaggtt tgctattgaa
     5461 caactgtgtc atacggtcaa gcacaatcgc caagataacc aaggcaagac cactgacaaa
     5521 tccactacca acatcagcgt gttgaagggc tgacaagact tcacgtccta agccaggagc
     5581 accaatcatg gagcctgtta ctaccatgga aagggctaac atcatggtct gattaacacc
     5641 tgccataata gtatttttag ctaaaggcaa ttccacttta aagagctttt gtttcccagt
     5701 tgacccaaag gcatctgagg cctcaatcaa ttctgttgga atgtcacgaa tggccaaatt
     5761 tgtaaaacga accgttggag gcaaggcaaa gatgactgag gcaaacactc caggcaccat
     5821 accaatacca aaaaaggcaa cggcaggaat caagtaaaca aaagccggca tggtttgcat
     5881 aaagtccaaa ataggattca caatctgacg gacagtggca tttttagcca tccaaatccc
     5941 cagcggaata ccaataagta ccgaaatcaa actagctact aacaccagag taaaggtatt
     6001 cattagctgc ttccataacc cttgattgta aataaagagc aatcctaaca aggtaaaggt
     6061 tggcaaaggc cattttttct tagcaaggaa aaacacccct gctgttacta gaacaataaa
     6121 aagtaacggg tggataaata aaagtgtttt ggtcatccaa tccatcaaaa agctaccaac
     6181 tacttgcata atatcaaata gacctgagaa agttttagtt agccactccg ttagctgctc
     6241 caccagttga gctactggta attttgtttg taatatcgtt tccaaaatgc atcactcctt
     6301 ttagttaagg tcttcgtctg agatattagc caaggcttca atcactcgtc ctcgaataat
     6361 gacacctagc agacgattat tatcatctgt cactgcaatc ggagcagatg aatcatagat
     6421 aagaggcaaa atatctgtaa taatagtatc ttttgagaca gttctcacat cgcgatcaat
     6481 cacttctgat agcggtaacc cttttttgcg cgcctctata gcggcatcgg ccgtcaaact
     6541 accgactaat tggcggcggc gattcgtcgc catcaacata gacacctctt cgttgtgcat
     6601 acggttcaag gcaacttgag gtccatctaa ttcaacagta gttgttaacg gtttgatcat
     6661 gatattttgt gctgtcaaga ctttagaacg gtccacatcc tcaacgaatt cacgcacaaa
     6721 gtcattggct gggttagtca agatttcctc accagtacca atttgcataa tttgtccgtc
     6781 tttcatcaaa gcaatccgat caccaatacg caaagcttca ttcaagtcat gactgataaa
     6841 gatgatggtt tgtttcatgc tgtcttgcaa atcaagtaat tcatcttgca tctcacgacg
     6901 aatcaaagga tcaagcgctg agaatgcctc gtccattaag agaattttag ggctattagc
     6961 tagcgcacgg gctaggccaa cacgctgctg catcccacca gatagttggt ttgggtactg
     7021 gtctttaaaa tctaataggc ctgaattatc aagggctttt tctgccaatc gctggcgttc
     7081 ttctttggga acgccacgta attccaaacc aaattcggta ttttccaaaa tggttttatg
     7141 aggaaagagg gcaaagcttt ggaaaaccat gttaatgtca tggcggcgca cctcacgcag
     7201 ctgatctgct gacatggttg agatgtcttt accttccagc aagatagatc ctgctgaagg
     7261 ttcaatcaaa cgatttagca tgcggacaag ggttgatttc ccacttcctg atagccccat
     7321 aataacaaag atttcacctt ttttgacctc aaaactagcg tcatagacac ctacagtagc
     7381 gcctgttttc ttaaaaatct cactcttatt tttgccagtc tttaccatct caagagctgc
     7441 tttttgtttt ttgccaaaaa ttttactgag atgtttgact tctaaaattg tttccattcg
     7501 ttttttctcc taggtaatcg tttattatgt gaaatagtga catagctttg gcgaaatatt
     7561 agccaatatt taacaaagta gctctacaca attaataaac gtgtggtcac ttttttactt
     7621 aaaaaaagaa tctgccgaaa cagaacataa ctatattatc acgtgtggtc actttttgtc
     7681 aaggacaata actcgatctg taataccaca atgataggtg atcgctttca ttcttttagg
     7741 tatggtcttt taaccagtac aattttttat cacaactgat gactatttct ttgctgatta
     7801 ggctattgtt acattagaaa tccttctttt acctataata tctcttttgc ggccatcgta
     7861 aataacgacc acggtgactc ttaaacattt gctcatctct ccaaaaaaac tcacctaaga
     7921 cagtgtccta tgaagcgagt tagcttaatc aggcactatc atcttaggtg agtagggtgt
     7981 tgcttatagt aaagctttaa attggatatt agaatcttct aagaagcagt catcaaatcc
     8041 acgaggatga tggtattcga tacgattttg gtcttgtgga taggtgtact tgccaccaac
     8101 ctcccagata aagggatgga aatcgtattg gagacgttct ttgcgcattt tccacagttc
     8161 aaggatttca tctgttgatg ccataaagtt tgaccaaatg tcataatgaa ctgggataac
     8221 gactttagca cggagatttt ctgccatacg aagaaggtca acagaagtca tcttgtcttg
     8281 aatcccaatt gggttttccc catagttatt taacacaacg tcaatatcat aatcacgtcc
     8341 atgtttagca aagtagttcg aaaaatgaga atccgcacca tgatagatgg taccacctgg
     8401 tgtttcaaaa atatagttaa cagctttgcg agccatgtca tcatctgtga tagctaaacc
     8461 tgctaaatca ccatcttgag catcagctcc ttgtattggg agagttacca gacaggtgcg
     8521 gtcaaacgac tctacagcgg tgatcttaat atctttgaat tcaaagctat cacctggctt
     8581 taagatcata atacgatctt ttggcacacc ccaatttttc caaacttcac cacattcata
     8641 aggtcctaca aatttaacat gattaagttt tgggttattg ataatggctg ccgcagtatt
     8701 gatatcgatg tggtcactat gataatgaga aaccaaataa taatccaact cattaatcat
     8761 aaatggatca atcaccattg gttgggccct aagattgggc tgtaatttac gagctcctgc
     8821 catattagcc atttgatgtc cacgaaccat atccttaact tgcttggtcg ctttcccacg
     8881 atttgaccat aagtccatga caacattagc accgcctggt gtcttaatcc agataccaca
     8941 gtttcctaac caccacatcg caaagttgtc agctggcacg acttcttgct cgatttcttc
     9001 atttaaccat gttccccatt ctggaaacgt gttaaggatc catgattcac gtgtaatatc
     9061 ttgtacttta gtcattaggg tttcctccga atttgttctt cgtttctata ctttaagtat
     9121 agactttctt gtcaatgata aaaagaccaa gtgtcacaca gatctgtgtt caaaagtgga
     9181 ctgacttttt cattagctca gtctaatact gtctcatttt gcacactgta tctcgcatca
     9241 atactgtatt caaagctata ttaaaaacct cagttatatt tttctatgtc atagaaaact
     9301 gaggtacttt gacgacttaa cacttactca tagcagctcg cgatgaatga gtttttcaat
     9361 tttagaaacc aaggcataac gatctgattc atcttttaca acagcttcaa tagcttttgt
     9421 gagctctgta gaaaagcggg agtgctcact caagcgtcct tgctttgaaa agcgtatcaa
     9481 tttgatgata tcatcgtcac ttaaaatagg gttaacaata agcatcggga ttttagtctt
     9541 tagcgtatct gtagtagcaa caatcatatc gactgctaaa aggtcgtaaa cactctgata
     9601 ctgttctgtc gtaaaaacag cttcaatctg accatttgct agataccttt gacactgttt
     9661 gaacaataac ttttgaatgc ctatcccatc atctgaaaca ataacgagtt tggtcttttc
     9721 ttgctctgta ttattgtgac gcagctctcc cccaagatgg atagtcaagt aggcaatatc
     9781 atcgtctgtc aagctaatcg tccaatcttg ttccaaaata gtggcacaag attgagtcat
     9841 agcaaacaat tcttcgtatt tttctttaat atggtctgtt aagggattaa ctaaaaaaat
     9901 cccataagct ttacggtaaa cgagcgcctt acagtgggtc gtcaactgct tcagcaaatc
     9961 ctgtttatgc gtgaaatgta actgataacg gctctccaac tggtcaataa agtgactaat
    10021 ggttgctcgc atgtcgtcat aatcttgact ttccacgtga tgatcttgat cttttcgaaa
    10081 agacaacatc aacatcgcta ccatactaac ttcgatatca tccaaatgaa gcttaaaatt
    10141 atggtaaagc tctctagcta aatcctgagc aatgtgatat tctttacgtt tccaaatcaa
    10201 atgaaattct tgtttaagag cagactttgt ttctgaatca agacgcatat tacgataact
    10261 taaaagcatg aaaggtaaaa tctgcaacat gaattggcta tcttgggtat taattgtctt
    10321 tcctaagcta gcttgagata aaaagagata ctcatgaaaa taagtcaaaa tatctttaga
    10381 aaaataaacc gataagcctt gtatctctga gagtttacgg ttgaatatat caataaagct
    10441 agtattgcca ccttggtaaa cgtccactaa caatttgtac aggtattgaa taagagccat
    10501 tggatgacaa ccaaaataat atcccctagc ctttgtcgca tgaagctgaa tcttgtattg
    10561 tttgtcttcc agttcttccc ttaactcagt caggtcattt aaaatcgtgt tacgagacac
    10621 atcattgatc tgcatcagtt tatcaatggt cacgcgctca gtggaaaccg caatataaat
    10681 cgaggatagg cgtctgcgct catcactttt catgacataa ttataatcag tcacttcttc
    10741 taataataag cgacaagcag ctttttgatc agctgttaaa agaatgccaa gtctaggata
    10801 agagataatc tgatctactc cctttggcaa agccttatta attttgtcaa gctggtaata
    10861 aactttacga cgtgactgat ttaaagcatg agaaatggcc ataactgtct caggtgtttc
    10921 tagtttaagt aaataagaca gcaagtcata actcttttta tctaaaatca tcatgattac
    10981 aaacctcctc tctgaaaatc catccttgct tattttatca tgatttcagt gactgcgcag
    11041 ccaagttttg aaaaactcac cataataaga ttcgcttaat atgattcgag ctaatcacct
    11101 caactgatcc aacaccaaca ccttatatct ggtctctaag aactccacaa catacgattc
    11161 atcaagtttg tgctcattgg cgtttaaaca gttcatcgaa aaggacaagt caaaaggact
    11221 tgcccaaaca atgattaaca aaaccttgaa ctttctggct tctagtgaga acctgtgcta
    11281 ataagccata tcgtgacctc atttttgacc gtaataagca tttgggccat gtttacgcaa
    11341 ataatgctta tccataatgt atcgtggcgc tggctctacg cgtggattga tttgttctgt
    11401 caagcgattc attttagcca cttcttctaa gacaaccgag tggtaaacgg cttgctctgg
    11461 ggttttcccc cacgtaaatg ggccatgatt gcgaaccacg attcctggaa ccgccatagg
    11521 atctaagcct cgcttgctaa actcttctaa aatcacgttg ccagtttctt gctcgtaagc
    11581 cccatcaact tctgcttttg tgagagaacg agcacacgga actggtccat agaagtagtc
    11641 agcatgcgta gtcccataaa acggaatatc acgacctgcc tgagcccaac caacagcttc
    11701 tgtggagtga gtgtggacaa taccgcccac ttctggccaa gctttataaa gttccacatg
    11761 ggttggcaga tctgaagaag ggttcaaatc accttcgacc acattcccat caaggtctgt
    11821 aacaaccatg ttttctggtg tcaagaggtc gtagtccacc ccagaaggct tgatgacaat
    11881 acgtcctaat tcacgacaaa cttcagagac attgccccac gtaaatttga caagaccgtg
    11941 ttgtggtaga gacttgttag ccgcacagac acgttcacgc atttcttgca agtttttcgc
    12001 catattatgc caaccccgct ttctcaataa gtgggtaaag gaagtcctga gcttctttga
    12061 tagcagcttt tgtttcctcc accgtgtcac aattttctga ccacatttca atcaaaaaag
    12121 gcccattgta gttggttttc ttgaggaccg caaacagctc ttgccagtcc acacaacctt
    12181 gaccaaatgg cacatcacgg aactgccctt ttgaggtttc cgtgacagca taagtatctt
    12241 tcaagtggag agctgcaatg gacttgtgac cattgtaaaa ttcactccac aaatcgttat
    12301 gccaggcaga aacattgcct gcatcaggat aaacaaagag ataaggcgag tcaatctctt
    12361 tttcaactgc caagtatttt tcaatagagt tgataaaagg gtcatccata atctcaatag
    12421 aaagcatcac ttgggcttct tcggcccagt cacaagattg tctcaagttt ttgataaaac
    12481 gagcacgggt ttcaggcgat ttttcttcat agtaaacgtc ataaccagct aattgaatgg
    12541 tacgaacgcc taagtcctgt gccaattcaa tacattgttt catcagttta agagacttgg
    12601 cttcagtagc aggatcattt gagcctagtg ggtaacgtct atgaccagaa aaacaaatgg
    12661 tcgggatgcg aatgcctgtt tcataaatgg ctttcactaa atcaagccgt tcttccttag
    12721 tccactctaa gcgcgctaag cgagcatctg attcatcgac agacatctcc acaaaatcaa
    12781 aacctaagtc ttttgcaaat tgcaagcgtt cacgccaggt aaactgtttt ggcgttgctt
    12841 tttcataaat accaatagga cgcgccataa agttaccccc agatacgttt gatttcatct
    12901 ttgaaggcac gcgccgctgc agcaggatct tctgcttcag taatgcctcg ccctgcgata
    12961 aaggtaaaga catcaacccc ttcaaaaagt ttcaaagtgt ctacatcaag cccaccagtt
    13021 actgagacac gaaagcccat gtcaatcaat tttttcacct tgttaaggtc tttttcaccc
    13081 caagtttctc cagcaagaag ggcatcacga gattggtggt aaatcgcttg ggaaatccca
    13141 gcgtcaagcc ataattgagc ttgctcataa gtccaatcac cataaagttc gatttgaatt
    13201 tccccacggt caccgcgctc ttctttaata gccttgaggg ctgcttccat ggttggaatc
    13261 gtcgcacagc aaatgcaggt catccagtca gcaccacgtt ttgcattgtt tttagcgacc
    13321 gtaccgccag cgtcagcaca tttggtatct gctacgataa ttttctctgg gaagaggctg
    13381 cgtaatacct caactagttc gcttcccact tgaagcaaac aaacggtacc agcttcaatc
    13441 acatctactt cgtgacccac agcaacggct gctttaacag ccccctgtaa gtcagaatgg
    13501 tctaaagcaa cttgtaaatt tggaatatgt gtcatctctt tttcctaaaa ctcttagcca
    13561 ccgtgcaaac tggttaagag ttcttatcct ttcaataatg attgtgagtc gttttatccg
    13621 tttaagtcag taagctatcg attaagcgtc taaatccaaa ccttctaaat acgggctatt
    13681 ttttgattct tctaccattt ctagaacctc tttaggactt tggcaggcca ccaagcgttc
    13741 aatggcgtta tctagttcaa acaaggccac aatttgtggg atagccactg ttgtgtgaat
    13801 agaagggtct gtagcggcca acgtcaaaag aacagaaacc tctttaccat ctgagaaggt
    13861 gactggtttt gttagtgtaa tcaaagcaaa tgcattgcga ttaacgccta aacctacttc
    13921 tgcatggggc atagccatac ctggcatcaa cacataataa gggccgtatt tttctgttga
    13981 tgcaatgatg gcgtcgtagt aagctgacgt cactgctttg ctatcaatca aaggttgaac
    14041 agctaattga acagcctctt gccaagtatc agcacttagc cccaatcgga tggagttgtt
    14101 gtcaataaag gcttgtttta aattcataaa aactatctct ttctagtgtc ttgctttgca
    14161 acacacacca tttttactag ttttatccta ctaaagagaa agagctgaaa accttcgtca
    14221 caactccttc gttttgattc agaattagct tatagcactt cttgtagttt ggttttaatt
    14281 tcattatcat ccatcaggtt atcaagacca accaagtgac ctttggtacg accatcaagc
    14341 tcatgaatca agtggtttga tgccactaca atatcataac cagaagctag acctttagct
    14401 tcaccaactg agcaagaagc tgactgaata tctgtcacac caagttgacg aagcgcattt
    14461 tcaactttca ttttgatcac catagatgac cccataccat tgccacatgc tgttaatact
    14521 ttaaccattg ttttttcctc cgaaaatgtt ttattattat tttgttagaa tagcagagct
    14581 atttcttaag atgcagtgtt attgagcttc cccacggtaa taagcttctt tgtccttagc
    14641 ttttgcgaat tgcaattgtg gaatggcaag taagaagatg caaacaagca cataccctgc
    14701 aataccaagg aatttgaaga ggtacccaaa tggtaaccat ggaagaacca agtcgatatt
    14761 accgtgataa ccacctgtaa gtcctaaaag acctacagca acagcaccaa gtgcaacttg
    14821 caaaatacct gagataaatg atagagctac cgctgctttc cagccaccac gtttatctgc
    14881 ataaaccgca attgccgcat tatcaaagaa tacgggaacg aatcctgtga taattaagat
    14941 tggattttta aagataacaa gaagagcaat ggtgattaat tgaccaatca aaccaaaggc
    15001 aaatcctgaa agcaccgcat ttgaagaacc aaagccatag gaagctgcaa cgtcaaccgc
    15061 tgggaatgac cctggaagca atttgcttga gatcccttga aaggcattgg tcaactcaga
    15121 aacgaacata cgtacacctt gcatcaaaat gaagaggtaa acagagaagg tgagtgatgt
    15181 ttgaagaata tacatgaaga aagcttgctt agctgggttg aaagcgcctg gtccgattaa
    15241 ctctacatta gacatgatgt ctggaccaag gacagccaag atagctccaa agaagactaa
    15301 cattaaggtt gctgacgcaa caacggtatc gtggaaaata tttaagaagg ttggtaattt
    15361 taagttatca aggttttctt cttttttacc aaagattgga gcaaccttat ccacaaacca
    15421 aatcgcaaat tgttgttggt gaccaatcgc aaagccaccg ccacctgtta ggcgttgtgt
    15481 tgcttcaacc gtcatgtttg agctgactgc ccagtaaaga ccacagatga taccaaccgc
    15541 ccaagcacca aaagcatttt ggaattgagg gattaaaaga aggacaaaga ccgaaatcgt
    15601 cgctgcctgt tggaccatga tgtgacctgt gatgaaaagg gtacgaactt tagtgacctt
    15661 gcgaagtgca accaaaagaa tgttgacccc aaatccaatc aagagagctg tcgtagcaac
    15721 actgatgaag cccatctctt ctaacttagt attagcagca gccaaaccaa aataaggatc
    15781 aataacggcg gctttcaatt caaatttctt ggcaagagca accaagatcg gacgaaaagt
    15841 cgttacaaga ccgcctgctc caacgttcaa aatcaagtaa ccaacggtag cctttacaaa
    15901 accagcaaag acctcataaa tgggtttttt aaggagtaaa tacccgatta aaaccaaaag
    15961 acctacaaag aacgctgggt tttgcaaaat attttgcgag aaccagttta ggggagctag
    16021 gagcatttcc ataagaacta cctcttttca aagttaataa agataaagtg tttatatgtt
    16081 cattataggt aatgaaagcg ttttaaaaaa gaccaactat cacacaaaga agtgttcaat
    16141 attagactta agttaactta ttttaaaatt taactacact taataataag gaggaaatac
    16201 ccctaaacaa cgattaaaaa ccacaagaac cttcgttctt gtggtttttc tagataagtt
    16261 gctttttgaa tagatcagct ctttggcatt aactgtgacg tgacgcaaat aaccactgaa
    16321 gggctgtgtc acctcaaact atttgataac aatattaacg agtttgtttg gtactgcgat
    16381 gactttgatg atgtctttgc ctgtgatttc ggcttggact ttttcgttag caagggcaac
    16441 ttcttgcaat tcttcgcgtg aaaggtcttt agcaacgaca agtttagctt tgaccttacc
    16501 tttgatttgg acaacgattt ccacatcgtt ttcaaccaat ttgcttttat cataacttgg
    16561 ccatggcacg taagagattg actcacctga ggctgtcaag gcttgccaga gttcctcacc
    16621 taagtgcggt gcaaatggtg caatcaattg aacaaagcct ttggcatagt cgctaaagag
    16681 tttgtcttcc ttgttggctg cgttgacaaa gaccatgagt tgagcgatag ctgtgttgaa
    16741 tttcatttgg tcaacttgct cagtaacggc cttaacggtt tcgttgtaaa ccttgtcaag
    16801 agctccgcta ttttcctcag tgatctcttt agttgtgatc aaacggtaaa cacggtccaa
    16861 gaacttacgt gagccttcaa gaccttcttc agaccaagcg atggaagcgt caagtggtcc
    16921 catgaacatc tcgtaaacac gaagagtgtc agcaccgtac tgttcaacaa catcatctgg
    16981 gttgacaacg ttcttaagtg atttagacat cttagctggt gcttgttcaa gctcttcacc
    17041 agtttccaca tggaagaagc tgccatcacg tttttcaacc ttgtcagtcg ctacaagcgc
    17101 tcctcggctg tcgcggtagc ttgttcccaa aatcatccct tggttaaaga gtttttggaa
    17161 tggctctttg gttggtacga caccgagatc gtaaagaacc ttgtgccaga agcgagcgta
    17221 gagaaggtga agcacggcat gctctgcacc accgacatag atatcaactg gcaaccattg
    17281 tttgaggagt tcctcgtcag ccagtttttc ggtgttgtgt ggatcgatgt aacgaagata
    17341 ataccagctt gaacctgccc attgtggcat ggtgttagtc tcacgacgac ctttaacacc
    17401 atcttcacga gtcacttcca accagtctgt cacgttagca agtgggctct caccagtacc
    17461 tgaaggacga atatccttag ttactggaag aaccagtggc aactcactct caggaaccgc
    17521 tgttgaggtc ccatcttccc agtggatgat tgggattggc tcaccccaat aacgttgacg
    17581 gctaaagagc cagtcacgga gacggtaagt cactttctca ttaccgacgc cttctgcttc
    17641 aagccattcg accattttgg caatagctga cgctttatcg agaccatcta ggaagcctga
    17701 gttgatgtga agaccgtctt ctgtaaaggc agcctcttca acattaccac cttcaagaac
    17761 agagatgatg tctaggttaa attgttttgc gaattcccag tcacgttcat cgtgggctgg
    17821 aacagccatg atagcaccag taccgtagct agcaaggaca tagtccgcaa tccaaactgg
    17881 catttcgtta ccattaactg ggttaatagc gtagctacct gtccagacac cagttttttc
    17941 tttggcaagg tctgtacgag caaggtctga tttgagactt gcttgacgtt tgtagtcagc
    18001 cacggcttct gcttggtcag ctgtcgtaat agcatcaacc aaggcatgct caggagcaag
    18061 aacggcgtaa gttgcaccaa aaagggtgtc aggacgtgtt gtaaagacag tgaagtcctt
    18121 atctgtatcc ttaaccttga aggttacatt ggcaccagtt gatttaccga tccagttgcg
    18181 ttgcatgtcc ttgattgatt ctggccagtc aacttcttcc aagtcttcta gcaagcgctc
    18241 tgcataggca gtgattttga gcatccattg gcgcatgggt tttctgacaa ctgggtagcc
    18301 accacgttct gatgtcccat cagggagcac ctcttcgttg gcgatagctg ttcccaattc
    18361 ttcaacccag ttaacgggaa cttcagcttc gtaggctagg cctttttcat aaagcttggt
    18421 aaagatccac tgcgtccact tgtagtagtt agggtcagtt gtgttaactt cacgatccca
    18481 gtcgtatgag aaaccaagag cattaatctg gcgtttgaag ttagcgatgt tttcagctgt
    18541 aaactcggct gggtcattac cagtatccat agcgtactgc tcagcaggaa gtccaaaggc
    18601 atcccaaccc attgggtgga ggacattgtg accttgggca cgtttgaaac ggctgagaat
    18661 atcagttgct gtatagcctt ctggatgacc aacgtggaga ccagctccag atgggtatgg
    18721 gaacatatct agggcataaa acttaggttt tgatgcgtct gttcctgttt taaaggtgtg
    18781 attatctgcc caaaaggctt gccatttggg ttcaatagcg gtgtgatcgt aaaatgtcat
    18841 gtgaaatctc ccatgtaaaa aatctagtct tcttattata gcatgagatg aagcttttag
    18901 gaatggtcaa gacatgacta gcgccttggc ttttgagact tcagacgatt agctattttt
    18961 aaaccctgct ttagcatcaa ccatcctcac aaaaagctaa taaaaaagcc agacttgctc
    19021 tggaaacaga gccaatctgg acaaatagac ttaaaaagca agtgcttgct caaaatcggc
    19081 caataagtct tcgatatttt caacaccaca agacaagcgc agcaaaccat caacaacccc
    19141 ttgttccaac cgggcttgtg acgataaaca agcatgagac atcgttgctg gatgtgacaa
    19201 aatggactct actccaccta gactaacggc taaaatcggc aactgaatac gatgggtaaa
    19261 gatcatcagc tcctccttag aagccaactc aaaggataaa acagcaccac cattcttagc
    19321 ttgttgcagg tgggtatcgt gattaggatg actgtctaag ccggggtaat gtactttaag
    19381 aactttgggg tgcttcgcta aatagttagc gagctgctga gcatttttta ccgcttgttc
    19441 catacggatc cccatagttt tcatgccacg aagaatcagc caagcgtctt cgaccccaac
    19501 aattgctcca aagttttttt gaaaaagctt gagctggttg tagatagcct cattattggt
    19561 agcagctaaa ccagctacca catcactatg accattgata aatttggtca cactttctac
    19621 cacaatatcc acacccatag ctaagggttc ttgatacaaa ctggtcataa aggtattatc
    19681 agccacagtt agcacaccat aagctttggc gagttgcaca agctctgaaa tatcacaaat
    19741 tttcaagagc ggatttgacg gtgtctctag ataaatcata cgcgtattag gcctgatact
    19801 tgcttttact gttgctaaat cagccatatc tacaaagctt gtttcaatct gataattggg
    19861 taaaatagct gtcgcaaatt gacaagtccc tccatagacc tccaatggga aaatcacatg
    19921 atctccagct tttaagagca tcaaaacagt tgaaatagca gccataccag atgcataagc
    19981 caccgcataa gtcgcttttt ctaaacaggc taacccgtct tctaaagctt cagtcgttgg
    20041 gtttgaaaag cgtgtgtaca aatgtttttg actaggacaa tacagttctg aattgtggaa
    20101 cgtcgaagtc tgatagatgg gaactgaagc tgctcctgta aactcgtcaa taaccgtata
    20161 gccatgtaat aaggtcgtat tttctctcat ttcgtgcacc tccgcaatag cgttaactcc
    20221 aatgaactct cttttagaca atggtcccac cccaatggtt gcacaaacca aaaagctctt
    20281 caaaaaagcg tgatctgcta acctataaga aaagtagtgg gcacctttta aacggataat
    20341 gaatttttcc aacaatatca tttttagatc gattacgttt attatacaac ctttagctaa
    20401 gtttgcaagc ccttttatca tctttgacca agctgccaac acctctccag caagttaaag
    20461 tatagttacc tttggcccca tcgtctcaaa agctcagctt atcacactga cagtcctact
    20521 ttgcatcgtc acctactcaa tatggcaata ttaccatcaa aatttcatta aaagatcaca
    20581 atttatttca tttaatgaaa aacagaactt gttttcacca tgcgccttat agtacactac
    20641 taaagtacta tttattttta ggagagaaat atagtgaaaa acaaattatt tttagttgcc
    20701 cttgcgaccg taactgtcct agggccctct ttagcaaccc ctcatcaccg gaccgtgcat
    20761 gcgactgaac agacagtaac tcaaagaaca gctactgaaa catgtacatt aaatggaaaa
    20821 atatgctatg gttatgaaaa tcccgatgaa gactacaccg atagcatggt tcaatctaag
    20881 tctcaagatc aaggtttctt cagttggctt tggacacaaa taacaacttg gttttcctca
    20941 ttaatgagct aaaaccatct cgtggcggtt ttctaatcct aggtcgccac atttcttatt
    21001 cagataaata ataataaaaa cattaaaata acacttgatt tattaacaaa aagttagtgt
    21061 aatataatca caatacaaat taaaggagtt atctatgaaa aagaaattat ctttatttat
    21121 gatcgcaact gctgctgtcg taggccttag ccttgcaaca ccagaaaacc aaactgtttc
    21181 agcaaatgca cagacaccag ctgttcaaaa ctgtgaaaac ggctgttgtg accgcccttt
    21241 ctttgggtat tgtggacctt tcagctggct ttactataat acaagatatt ttttggggta
    21301 ttctgataca gctactaacc aataaatttg tttctatagt gagacaaaag cccaagcctg
    21361 tttacgcagt cctgggcttt tttgctgtcc taaagtcagg gtgttgccac agcttcaaat
    21421 ccaaaaacaa ttaaaccgca ccccaacaat tagacatagg ctatgtctcc agaatcctgg
    21481 acacatatca ctaaagccta atgattaagg tgcagtttaa ggtgtggtag caaggaactg
    21541 ttttaaattc gtctgcgtct aactataccc actggctatg gagtcagctt agagttgatt
    21601 atcaagtgtc aaatcaactt ttcccaccaa tgtaaaatga gtccccatat aaacttgaca
    21661 ggaagtaaca aagggtagct tcccccaaat ccttcattat aaccctcttt gtacccatct
    21721 ttataaccat ctctataatg ctcatgatta gagtcatcaa tatccttagg tgattttggt
    21781 acttcagggt caggtggggt caaatcggtc cattcggcta cttttccatc ttctttcccc
    21841 tttgttaaat cttggttata gcctaatctc caagggtctt tatcctcctc atctgccata
    21901 acaggtcttg ctataagcaa catagcagta agtagagcca tcaagaggta gacgaagtgt
    21961 agtggcttgt aagattgttg tttcatgata atagttatct ccccctttta tgttatgctt
    22021 tgcttgttaa aaagcatctt gttagtatta tagattagaa gtggcttagc ttcaaggtaa
    22081 ctatgtaaga aaagaataac tttttgatta gcatatcaaa aaacttgtct atgaaaatat
    22141 ctcatagaca agctttttgt atttgtgtga ggagtaggca catggcctca agagataagc
    22201 agcatcaagg tgcttgacga acctcttgaa ccagtcctac ttataagtaa tcgaaccata
    22261 tgggctcaag gttgatcctg agatgttgac attgatttct ttagacagtt tcacatctct
    22321 ttcgtcgatc acttttcgcc accattccca agctaatccg gtgcactctc tagccatgat
    22381 acggatattt cgtgaattag ctcctagtgg gataactgtg ctaaatggtg atgtcttact
    22441 ataccagttg ttgtcccaac gtcgttttgt aatcacttct tttcctttgt catcataatt
    22501 gatttcatcc caaaggattt catattgagc aacataggca ccttgatgag acaggttaat
    22561 ttttccactc gtgtactcgg tagatgttgt ttcaacgtat tcacttctgt tattgacacc
    22621 cgcaatttta ttatttttaa ggaaaacact ggtgtatgaa ataggataag ctgggttttt
    22681 tctactgaag gtagcattat ctttgataac gtttctaata acatcaaagt cttttgtgac
    22741 taccttattg tgctctgcag catctcctcc taaaacgaca gctgtaaatg aactattttc
    22801 taaaatatca gagtattttc cattagtttt aacatctgtt ccttttagag ctgcactaaa
    22861 ggccgcttca acatcattac ttttagaact tgtttctagt ttgacaaaaa cagttcgacc
    22921 ataagctacg ttactcacaa agagtggcgg ggcttcattg ctgacacctt ttcgttgcaa
    22981 ctctttaaag gtcactgatt tatcaaacac atccgcagga ttattaggaa ggtttgctga
    23041 tacggtgtaa aaaatttgct tgtatgctgc aatcatcacc ttcttttcac cttttgaaat
    23101 cgacttgaaa tcaatgccta aagtaccatc taagatttta ctattaacat ttagagctgc
    23161 ttcaatctgt gatttagaat ataccattga ttcagtatat tgtgttctgg caggaagcgt
    23221 attaccacca gaataattat catgccattg gttaacaaga ttatcaatag ctgttgaaac
    23281 attggcatag gtagggtcat tgacctcaac cgttgctttg tctcccatac ctggtaaatc
    23341 aatatggatt ttttgtgggt ttcgcttggt gactactgcg tctggtttgt tttcggtaaa
    23401 acctttatta gccagctgaa gggctgctgg ataggtccta tcagtgacag agtcaatgat
    23461 ggaaatatcg accggtgtag tgttgatatt ttttttcttt ctttcaatga caataaattt
    23521 gtcagctttc ttaacgcctt ctttaggaac aaagttttca atggtttcac catttttagc
    23581 aagtacttca agctcattat aatttagtga ataaatcttg tcattgattt cttcagtatg
    23641 atcttcttcg ctttttttat tgtcttctga ctttttttct tctttttctg cagattctag
    23701 tggcatttct ttgggagcaa gcttaatcat atcgttagag ttaagcatat catccatttt
    23761 ctgacctgct ttttctgtag ttagctcact actttctggt tttggttgtt catttgtcgt
    23821 tgtggtttct gtattggcag tgttttgttt gttcgagtca gcattagcag taacaagatt
    23881 accaacgata agagctgccg ttaatagccc agcgacgcga ctgtattttt taaatatttt
    23941 tttattagac atgtccttca taccttttta tcattctaaa atgtttctat tgttctttcg
    24001 accatatcaa gcatagtatc ttgatagtga gcaaattctc ctaatttgta atgtgccata
    24061 gcctctctaa tatgcgcatc ttttaacgct ttggttatat tagctagtat gctttgacgg
    24121 tttaaataaa atggcaagct ttcgtaataa tttaataaca tgttttcagc gtttagggtt
    24181 tgaaccgctt ggtaaatgtc taatgcttga ccgctatttg acatttttaa taatgcttct
    24241 tcgatgttag ctttcaattg agtatagcct tttatgaaag cagggtaaat cccatactct
    24301 aaaataactt ctggatcaga aatatctccc atataggagt ctcttttagt gatacgatac
    24361 tctttgtcac tacctatcaa gtattttttt aaatcattaa cagttacttc tttttgaaac
    24421 attttttggt aatgttctaa accctttggc accttataca tattgttact tcctatcttg
    24481 cattttctta attttgaagc gaccagttac ttttttatgg tcaccatttg gagcaatgat
    24541 ggtaggatca ataatctcaa aatagccttt ggcatcaatc tctgctaaga cagctttaga
    24601 ttttagatct ttaacactaa tcgatgggaa aattaattca ctttctgaat tttcaatgtg
    24661 ttttcctgac cacgtttggc aaccaggaat gttgagcgaa tttgctccag gagtatctcc
    24721 gctagctgtt cggatatagc ctagcttatc aatgtttttt gttagttcag aatcgtactc
    24781 atattgataa accccatcgc tatattttcc gctatcgaca ccttttatat tttcaatttg
    24841 gtcagctgat tttaacttac cttgatcagt aaagagcaat gccatatctt cagcaaaact
    24901 tgtcacaaaa agaccgtttt cttgtcgacc aatcgtcgac tttcttttag catcatcaaa
    24961 agctaacatc aaatcttttt tgcttgtaac gtgggaagct ccgctttctt tgtagacttg
    25021 ttttagtttt tcaacaaact cttttttgta gtggtatttg cggctaacgt acatacggta
    25081 atactcagca cgtgacgcaa cagcagacat ctgaacagct ttatcagcat acttgtcggc
    25141 ggtttcaata aactcatctg cttgtttatc tgttacccat ttatctttaa tttctttaaa
    25201 ctttttttca aaaatatcgc gttccatatt gccaaaagtg acctctgaca aggctaaaat
    25261 atccttgttt tgaccctgat ggacctctgt tacctcaata tcaatacgat caacatctaa
    25321 ttttttatca atctcaatgt gatgcggttt tttagtgtag gtgtcgccta attcaatttt
    25381 ttgaaagagg accaatttat tcccgttata gtatttaaca tcagcctttg caattctatt
    25441 tttcttgtag taattctcct tgctagatgt atctccatta acaattaaaa ttctcccaat
    25501 atgcatcggg gacgcaaact tgtaagaaag tacctcacca ataccaccgc caggtgaatt
    25561 ttcctcccaa acagtactca ttgagccgtc taatgtgtgc atgacatgtt tgtcttcttt
    25621 agaggaagtg gcgttagctt ccataacttt ggtcatttca tatttgacat cgcttttttt
    25681 accttctttg ccactaacag cgtacgttgt gttattagct aataaactgc tacccattgc
    25741 tccagccaaa gcaatagcaa cacttgtctt cgcgacaata tgagctaatg ttactttttt
    25801 gtttctcatg taaaccacct tatattattt ttaagtaaac tatacattaa caaatagttt
    25861 ttgtcaatgc gatttattaa catcgtttta ggattttcta taaaaaaaat gaacaaaaaa
    25921 caagtaggca actgttacag ttttcaaggt aatatgtgca tcttatcatc acagaacctt
    25981 cacatctgtt ctatttaaac cagattaaaa gtactagtta agtatttgat agtagcagcg
    26041 ttatcagtaa tagatctgtt aattatctct gattacaatg atgttaacaa tagtcatacc
    26101 tttatttgta cacacgcttc acttccaact aaaaaaggtc tgatggtaat acttatacca
    26161 tcagaccttt gagagcctat agttctgcga tttggtaaag ttcaacttcg gctactgttt
    26221 ctgatccaaa catgttcaac atcagtttca ccttgttatt ttcaatctca accacacgac
    26281 cttcttgtcc cataaaggca ccgtcaatga tttgaaccac atcgccctct ttgatgttgg
    26341 tatcaaagac atcaatggtt tgccccatag ataataggat ggcacggatt tcttcttcaa
    26401 gtagtggcgt tggtttagaa cggttcccgt gtgaacccac gaacccagta acgtttggcg
    26461 tgttacgcac cacgaaccaa gcttcatctg tcatgaccat ttctaccaat acataccctg
    26521 ggaagcggtt ttcttcgatt tctttggttt gcccattttt ttcaacgtta accgtttgtg
    26581 tcggaatttc gacgcgcaaa atattgtcca acatgttata ggtttgggca cgttgcaaga
    26641 ggttttcttt tactttgttt tcgtagcctg agtaagtttg gaggacgaac cagcccttat
    26701 caaatgaatc tagcatgatg tttcctttca ttataaaaaa gccctttttt tggcttttgc
    26761 gctgttttct atcaccttca ttataataaa agacagggct gctgtcaacg tatctgtgga
    26821 gctacttgac acctttatta aaaagtccaa agtcggttcg tgacgactct ggacggtatt
    26881 gtacagtttt agttttcagg aactgtgatg gttccttcga taatcgcttt tttagcagcc
    26941 tcaatagctt ttttagtgtc ttgtgtcaga gcatccgttg taaggctaac accaccttct
    27001 tttaaaccaa aagtggttat ttgaccacct gggaattggc cttctgaggt ttttacggct
    27061 acttttacta aagcttttcc gacttccttg atacttgagg tcaaaacaaa attagctgac
    27121 ttgccatctt ttgtggtgta ttttccatct tcactttggt cacggtcaac accaataacc
    27181 caaaccttat cttcttcttt acgtttttcg ttgatagact tagcttcgct aaagacaccc
    27241 gctcctgtgc ctcctgctgc atgataaata acatctgcgc cttcagcgta ttgagcagct
    27301 gcaatcgtct tgcctttagc agcatctgca aaagagcctg cataagcaac tcttactttg
    27361 atggtatcat ctactgattt cacaccagct tcaaaacctt tttcaaagcg cttgacaaca
    27421 tctccttcca taccacctac aaaaccaact tgcttggttt ttgtcgtttt agctgctgca
    27481 acaccggcta ggtatgccgc ttcatggtct gaaaaggtga tacttgcaac atttttttga
    27541 cctttaatca catcatccac aattgcaaaa tggttgtcag gattgtttgc ggctactttt
    27601 tctacagcgt catgtaatgg aaatccaatc ccaaaaacaa gattataacc attggttact
    27661 gctgactcaa ggtttgtggt aaactctgat tcattggctg attggaaata atcatagcct
    27721 tttccttttt caagtttatt ttcttttccc caagcttgca agccttccca ggctgactgg
    27781 ttaaatgact tgtcatcaat acccgtctga ttagtaatca tagcgatttt taatgaattg
    27841 tcagttgttt gtttagcgga gttattggta cagcctccta atgtgaatag ggcagtcgaa
    27901 acaagaccaa gtgacattac ttttttgttc atgtgaaaat gaacctccta aatgtttaat
    27961 cctaaagtga tgtgtcattg ttaacgttct acttatgctc tagaacgaaa agtttagtat
    28021 atcaaaaaaa tcagttttat cctactattt ttgttgaaaa atctcttttt caccaaaatg
    28081 accgaacgat aaagtgagat caactggtaa atcccattat ctcctcaaaa atcacctctt
    28141 aaggagataa gtcaacgaca aaactcaagc tactagtaat agcatgacta actataccat
    28201 ccaacaagcg gtaaaaagag ctggaaacgc tattcaactc tttgtcatac tagacacctt
    28261 agatagatgc ccagaccgac tcaagaatat tggtctgttc acgtcctgga ccaactgaga
    28321 aggttgaaat gcgaacgcca accaattctc caacacgacg gacatagttg cgggcatttt
    28381 caggcagctc atcaaggcta cgaacacccg tgatgtcctc ttgccagcct ggtaattctt
    28441 catagattgg tttgcaacgt ttgagttgtt caagacttgc tgggtagtga tcaatacgtt
    28501 caccatctaa atcgtaagcc acacaaatct taaccgtatc aagtcctgaa agaacgtcaa
    28561 tggaattgag ggaaaggtta gtaatccctg acacacgacg actatggcgc ataacaactg
    28621 agtcaaacca acctacacga cgtggacgac ctgttgtcgt accgtactca tgaccaactt
    28681 cacggatacg ttcacccact tcatcaaaga gttctgtagg gaatggccca tcaccgacac
    28741 ggcttgtgta ggctttacag acgccaacta ctttgttgat tttacttggg ccaacaccag
    28801 aaccaatggt tacaccacca gcaactgggt ttgaagacgt tacaaatggg taagttcctt
    28861 ggtcaatatc aagcataacc ccttgtgcac cttcaaaaag aacacgttta ccggcatcaa
    28921 gtgcatcatt taaaattaca gaagtatctg tcacatattg cttgatttct tgaccgtaag
    28981 catagtattc ttcaaaaata gcatcaaaat caagaggtgt actgtcatac attttttcaa
    29041 ataaacggtt tttctcagct aaattaatgc gtaggcgctc tgcaaagata tccttatcca
    29101 gtaaatctgc gatacggata ccaactcgag cagctttatc catataagct ggaccaatcc
    29161 ctttaatagt tgttccgatt ttattgtcac cttttgcatc ttcttgcagt tgatccagct
    29221 ggatatgata aggtaaaatg acatgagctc ggtctgaaat gcgaaggtta tctgtcgtca
    29281 ctccttcatc atgcaggtac gccaactctt tgaccaaaga tttggggttg accacaacac
    29341 cattaccaat aacggaaatt ttttgcggga agaaaattcc tgatgggatc aagtgaagtt
    29401 taaacttctt accatcaatc acgatcgtat gaccagcatt atcaccacct tgatagcgag
    29461 caattacttc tgcatccgct gacaagaaat cagtgatttt acctttacct tcgtcacccc
    29521 actgggtacc aacaacaact acagatgtca tatcattgtc ctaactttct gaaacactta
    29581 ttctcaataa aaatcatagg caagctaacc actgcttaaa aaccaaaaag aaggtcagat
    29641 ctgtaagatc agcaatgtag ctcttgattt ttaaagaaaa caggcaggaa tctcacctgc
    29701 tagcatatat cttacaactt attatagcaa aaaatgattt tttctgctag atttcttagc
    29761 attattaagg aaaaagagaa actctttttt taaccatgat aaacaatcct aaaaaaacga
    29821 acattataag agaaaagctt ttaacctcgt ttctcctaag ctttagcagg gttaaacgat
    29881 tctcttaaaa cattagctct tttctttcag aaatagcggt tttaaggtaa aataatagta
    29941 agttacctct atgagatagc cagtaaaaac atatcgagga aatatcacat gattagaaag
    30001 tatgaccgca cgagcacaaa aaagaaatca ctaaactgga tttggctaat catcgctttt
    30061 tttatgattt caagctttat aggtggctct tcttttactg agtcacttct agacattttg
    30121 cctgccatag ccattggtgg tacaggttat gctatttttc gtgttagatc tcatcaaaag
    30181 cggctagcaa aggccaaggt tgccaaacaa ttagaagatc taaaagcaaa aattcagcta
    30241 gctgatcgta aagttcggct gttagatact tatttggcag accatgatga cttccaatat
    30301 aacgtactag ctcagcaatt attaccacaa cttagtgaca tcaaagcaaa agctattacg
    30361 ttaaaagacc agttagatcc caaaatttat cgtcgtatca ccaaaaaagc caatgacgtt
    30421 gaaagtgata ttacccttca actcgaaaca ctgcagattg ccacaactct taaccctcag
    30481 cctttgaaaa ccccatctcc taatcttatt aacaaagcac ctgagctcaa accgtattat
    30541 gataatattc agacagatca tcaagctatc cttgctaaaa tccaaggagc cgacaatcaa
    30601 gaagaattgc ttgcgctgca tgaagctaac atgaggaggt ttgaggatat cttaacaggc
    30661 tacttaaaaa tcaaagaaga gcctaaaaat tactataacg ctgctgctcg tttagaacaa
    30721 gccaaacaag ctatccaaca atttgatgaa aatcttgacg aaacccttcg tcgactcaat
    30781 gaaagtgatc ttaaagattt tgatattagc cttcgaatca tgcaaggtgc tactcaaaga
    30841 cgaacaacgc atcatcaaaa agactaaagg agtcccttta tggcagattt taattttgac
    30901 attgaccaaa ttgctgacaa tgctgttgtc aaaaccgata aaaccaccga tattatctca
    30961 gacttaccaa ctgacacaaa tggccaaatc tctttttttg agaaactaag tgctgaccag
    31021 cagacagcca ttactgccag agccccggca ttggtagata cctttttggc agaccaaaat
    31081 gcgctacttg attttggtca gtctgctgtt gaaggggtca atgcaacggt caatcatatc
    31141 ctggccgaac aaaagaagtt acagattcct caggttgatg atcttttaaa atcaaccaat
    31201 cgtgaactca atggttttat cgctaaatac aaggatgcca cacctgtgga ccttgataaa
    31261 aagccaaact ttcttcaaaa actctttaaa caaagccgtg ataccctgca agagttttat
    31321 tttgattctc aaaacattga gcaaaaaatg gatagtatgg ctgcagcggt tgtcaagcaa
    31381 gaagatacct tagctcgcaa tattgtctca gcagaattgt taattgaaga taatactaag
    31441 tccattgaac atttggtagg cgtcattgct tttatcgaag ctagtcaaaa agaagctagt
    31501 cagcgcgctg cggctttaca aaaagaccta aaaaccaaag acagtgctac accagactat
    31561 caaataaaag ctgatctttt ggcaagaact accgaagtca tcaacaccct cgaacaacaa
    31621 cacaccgaat acttgagtcg cctctatgtt gcttgggcga caactccgca aatgcgtaat
    31681 ctagtcaagg tctcttctga catgcgtcaa aaactaggca tgttacgccg caataccatt
    31741 ccaacgatga aactatctat tgctcagtta ggcatgatgc aacaatcggt taaatctgga
    31801 atgactgctg atgccattat taatgctaat aatgctgccc ttcagatgct agcagaaaca
    31861 agtaaagaag ccatcccagc attggaacaa tcagcacaaa accctacttt gtctatgaaa
    31921 tctgtgacaa gtctggcaga aagtttagta gcacaaaata atgggatcat tgcggcgatt
    31981 gatcatggac gcaaagaacg agcacaacta gaaagtgcta ttatcaggtc tgccgaaacc
    32041 attaacgatt ctgtcaaact acgtgaccaa aatattgtgc aagcacttct tagtgaaggc
    32101 aaggaaactc aaaaaacaat tgataaaaca actcgcgcat aagctaaaca tcacctgtca
    32161 cgctacttaa aaagcaagcc atcagttagg ttaatccctt tgatggcttg cttttttact
    32221 acttaacgaa taatcgcctt gtttgatata acacttatct tttatgatgt tattttcata
    32281 atacgaacaa ggctagcacg ctcggcctct tctaatttca actcaatgta atgaatcgtc
    32341 tctttcagtt gcgggataat agaatactct aagccattga cgcgacgtct agtcttttca
    32401 atgtcatcag ccattagttg acaagttttt tcaacttcag ctaaccgtaa gagcttttct
    32461 aatagttctt ttgtcttttg gatagtattg tccatctcac tatttgagct gaggtaacta
    32521 taagcaaact caccttgttc ttgctctctt gccgtattag actggacatg gaatttaggc
    32581 acattgacac tcatgatatt ttcaatatca atccataagg tcacttcatg gactggaaca
    32641 gcaaagagtt cttctaccat tagactattt tcggatgctt ttgccaagac aaactccttc
    32701 atattggcag caagttcttt ttcgatagtt tgacgaagct cattgttttc acgaattaaa
    32761 tcaacgaagc ggcgcatcaa ctcatcccgc ttgtccttta ataatttatg acctcttgta
    32821 gctgtcttca agcgattttt caagttacta agttccatgc gtgttggttt aacatttaat
    32881 cgtgccattg atataactcc tttcgtcaag gacttgaaat tgcatggtca actcaatcat
    32941 ttgcaacaaa aacctttgtc atcgtggtat ctgctttagg caaataacga tctaacatat
    33001 catctttaat gcgttttaat tcggtacgtg gcaaaatcga caacaattcc caacccaagt
    33061 caagactttc ttcaatgcta cggttggtgt aaaatccttg gttaatgtat tcttcttcaa
    33121 accggttggt aaaggccaca tatagtttat ccgtttcaga aagggctgat tcaccgagca
    33181 caacagccaa ttccttagct tgttttcctt gggcataggc tgcaaagagc tgattcattg
    33241 tggcagcatg gtcttgtcga gtcttacctt cacctgagcc tttatctttt aggcgagata
    33301 gcgatggcaa gacattaata ggcggtctaa aaccattttt atatagttct tgcgacaaaa
    33361 taatctgacc ttcggtaata taacctgtca aatcggggat agggtgggta atgtcatctt
    33421 ctggcatggt taaaatagga atttgagtca ctgacccttt tttcccgatc aagcgaccag
    33481 cccgttcata taaggtggag agattagtat agagatagcc aggataacct cgtctgcctg
    33541 gaacttctct gcgagcggct gatacttctc gtaaggcttc acaataattt gtcatatctg
    33601 tcatgatgac taaaacatgc ataccttttt cataagccaa gtattcggct gttgtcagag
    33661 ctatacgtgg tgttgcaatg cgttcaatag cagggtcatt agccaaattg ataaataaaa
    33721 cagatcgatc aatagcaccc gtttcacgca agtcattcat aaagaattct gcctcttcaa
    33781 aagtaatacc catagctgca aacacaaccg caaagttatc atcagagttt agaacagttg
    33841 cctgacgagc aatctgtgcg gccaattcat tatgaggtaa ccctgagccc gaaaaaactg
    33901 gtaacttttg accacgaacg agggtattga gatgatcaat agctgagata cctgtctgga
    33961 taaattcgtc tgggtaatca cgagcgacag gattgatcgc ttgaccatcg atatcgaggt
    34021 atttttcggg aataagttct ggaccgccgt ctatgggctg ccccattcca tcaaagatgc
    34081 gccctaccat atcttctgat acggctaatt ccaaaggatg ccctgtaaat cgaaccttgg
    34141 ttttggctag gttgatgcca gttgatcctt caaatagctg taccatagcc ttatcacctt
    34201 ggacttctaa gacttgacct ttacgccttt ctccattatg caaagtaata tctaccaatt
    34261 cgttgtagtg cactcctgca acttgatcaa caatcatcaa agggccaacc acttcgctaa
    34321 ccgtccgata ttctttgaga acgctcatct aaattcctcc ctttgctaaa acgtggtgaa
    34381 tctctttggt aaccttctga gtaagccctt taatctgctc taagttttct tctgggataa
    34441 atttgctgcg tgcgatgcga tcgcgtacct gagcagtgcc ttccataatc tcattaaaat
    34501 aagctcccaa agaaagggct ttgctggctt cctcattaaa ggtcaaaata ttggttagca
    34561 tggcctcttg tttcggaaag gaagtaaagg tatccaccga atcaaaggca ttttgctgga
    34621 gataatcctc ccgaatttgc cgggcaacag ccatggtcaa acgatcttgt tcagacagtg
    34681 aatcaagccc caccaagcgt acaatttctt ctagactggc ttcacgctgc aaaatagcca
    34741 tggcacgagt taccttgttg gaccaattag attgctgttt acggtcaata tagcttccta
    34801 catcatcttg atacaatgaa taagacgtca gccagttaat cgctgggaag tgacgccgtt
    34861 gcgcaagagg cgcgtcgagc ccccagaaaa ctttgacaat ccgaagggtg ttttgagtga
    34921 caggctctga aatatcccct ccaggaggag aaaccgcgcc gatggctgta atggttccct
    34981 cacgttcttg acttcccaag gtccgaacac gaccagcccg ttcataatat tcggcaatgc
    35041 gactccctaa gtaagccggg tagccttcat caccaggcat ttcttgtagg cgtcctgaca
    35101 tctcgcgcag agcttctgcc caacgtgatg tcgagtctgc catgatagcc acagaatagc
    35161 ccatatcacg gaaatattcg gcaatggtaa tgcctgtgta aatcgacgct tcacgcgctg
    35221 ctactggcat attagaggtg tttgcaatta acaccgtgcg ctccataatg gactggcctg
    35281 tatttgggtc aattaactct ggaaactcat tcaaaacgtc ggtcatctcg ttgccgcgtt
    35341 ccccacaacc gacataaata acaatatcaa cgttggcaaa tttagctatt tgatgctgca
    35401 caactgtttt tcctgcccca aatggtccag gaacggcagc ggcaccacct tttgtaacag
    35461 ggaaaaaggt gtcaataacc cgttgacctg taaccaaagg ctcgactggg ataaactttt
    35521 gagcaacagg gcgactttga cgaactggcc aagtctgcat gaggctacct tggtaaatgg
    35581 aaccgtcttc ctgcttaatt tcataaactg tatctgttac tgtgaagtcc cctgcatgaa
    35641 tagccaccaa ggtccctgaa actttataag gaaccatgat acgatgctca atgacagctg
    35701 tttcttgcac agttcctaaa atatcacctg caactacttc ttgaccaaca cttagcttgg
    35761 gaataaatgc ccacttagcc tttcgatcaa gacttgggat agccacacca cggattaaaa
    35821 agtcgctgtc cgttgctttt tgaaaacgat caagcggtcg ctgaataccg tcaaacattt
    35881 ctgaaatcag gcctgggcct aactcgaccg acaaaggaca accagtagtc actactggtt
    35941 ctcctggacc gatccctgat gtttcctcat aaacctgaat agacgcttga tcgcggcgca
    36001 tttcaataat ttctccgact aagccaagat gtcccacacg acaaatatct tgaatattag
    36061 cttcttgcat cccagaagcc acaacaagag gtcccgaaac ggttattatt tttccttggt
    36121 tcaccttttt cctcctttat tttttccaaa tcatactaac taatctatac tgtcaaggtt
    36181 taatgtgact attttgaata gtcagcaaac tatcctatgc gttcagctgc taacttcacc
    36241 aattttcctg tccttgcatt tcatcagtta gctactcata agcctcaaac agagtataag
    36301 tcctctttgc ctaacgtctt agtgttataa aatattttga ccgacagcct tttcaaccat
    36361 ggcttgtatg cgtgacaggc ctatcccttg cgctccttga tgagttggta atggtatgac
    36421 tgctggtaga acttgattat catagtgagt gagggcttct ggaatagctg cagcaatatc
    36481 ttctgtgata taaataatac caaagtcttc cattgctagt tgacgcaact ggttaatagc
    36541 gtcttgtggt tttattactg gaaaagtttg aaacccaatc atctgaaaag gtaaaataac
    36601 atctctatta ccaattaccc cgactttata caatgttttg gtcatgctat tggtctcatc
    36661 ctttctatga cacgttcttt gggtaaatca ttagctaaag cagccgctaa aagccgtagg
    36721 ttttttactt caaacgattt agcgagcaaa aagcgagcaa gcacataagg cccatcgact
    36781 tgatatttgg ccttcgcaaa taaatggtaa aggcattcat caacaaggta ttctaactca
    36841 gtcgtttgaa gagtgccttg gcgaagtttc tctgaataag gttttagggc tgtcatatag
    36901 ctatcaggtg ttacattttc aaaccacgtc atcagatcct tatcatcttc aagtgtgata
    36961 aatgttttac ttggtctact tgcttcatca gataataact gcatcatgaa acttattggt
    37021 ttattttgcg atttggcccg tctgacggta atcagattat acaaatcaat cacaataaga
    37081 acagcttgtt ggaaaacagg gtcctctaat ctttgtgcta tttgtttcaa cgccttaaaa
    37141 taagctaagt cagtaccaat ttctaagacg cgtatgtctt gataatcttg atattcggcc
    37201 caaatggact ggatttctgt taccacctct tttggaaact catcagaagt catcgtccta
    37261 atcaaatgtt ccaaagccac caaaggcttg tcaccaattg gtagtaataa gtgactcaga
    37321 tctgcttgac tagctttagc ttttaacaac actttgacat tatgataggt gtaacgcaag
    37381 gtaaataatt gtactatgtc aggttgaggg gtttctgcaa aagcccagcg ataggtcttg
    37441 gttagctcag ccattaagat agattctagc cgatccaaat cgttgagatc gtcaacagtt
    37501 aggtgataga ctgactggtg caacagtcga gctaaggttg ttgtattggg agcctgcaat
    37561 agcttatcaa attgctcttt agtcaataac tctttttctt tgacactgat agtagtgttt
    37621 aattctagaa atgttggcat cgctagtcaa acctcctata tctctgaaaa aagcatatca
    37681 atgatgcggg agctttcttc tttaagaact gattctaaaa ggtaacggta taaataattg
    37741 tcatcgactt ggtctaaact gactaaaaat cctgcttcct gaggaattag ctcttgatta
    37801 tagcttaatt gaggaaatgc taatctcagc tcagcacaat cataagaact gaacttttgc
    37861 cctgtttttt cgccaaaagt aacttgcaat ggctgttctg gatatttaga caagacttgc
    37921 ttcaaaaaag ccaattcttc ttccttagaa aagttcgtca tcttctccaa agaagcttca
    37981 aatagctcct ttatactgtc ttgttttaat gctagtagag cttgtctttc ttggtttttg
    38041 agttgctgct gcgcaacctg aaattgctgt tcaaaagctt ttaactcttt ttgacgcttt
    38101 gcttttaaat cgtgtagtcc ttgctgttga cgctctttaa agtcagtatc aagactatct
    38161 gtcgctatct ttaggcactg ttgaccttct tgatgtgctt tttcaagaac gttttgtctt
    38221 aactgtgtta tatcattcac ctaatttccc ccttttgata gtgttagtgg tgtcaccgtt
    38281 ttttaaaatc tagataagga ccgttacctt atccaatttt atttaagaga ataaaggaga
    38341 cgacaaaggc aagaatagca taggtttcaa ccatagctgc taaaatgaca cccttcataa
    38401 aatctttagg gcgcttagcc aagatttgca taccagctac agaaacattg ccttggtgct
    38461 tggccgaaaa ataaccaaca atagcaattg gaagagatac caaaaagtaa gctaatcctt
    38521 ggttaactga aagttctggt gtcagtttca tccaaattaa aataccaata gcaaagccgt
    38581 agattccttg actaccgggt aacagttgca agataagcgc agacgtaaat ttttcgggtt
    38641 cttcttttaa gagcgcagct gccgcttgac cacctttacc gacaccataa gctgagccca
    38701 tgccactaag agctacggct aaaacaattc ctaaggctgc gaagaaaacg ccaccatgtg
    38761 ctgtaaaata atgtgctaaa tgttccatgt taattgtcct ccaaatctgt ttcttcattg
    38821 acattgacat aattatcagc taactttaaa ggattgaaag cttttcctcc accttcataa
    38881 aattttccaa aaaactctac gaaaataaga cgagcaccat gcacatatcc tgaaagcatt
    38941 gataagaaaa tattgatcgc atgcaaaaga ataaaaataa atatacctac tgtaaagcgc
    39001 gtgactggtg gaaaaatccc aacaatcatg ttaaaagccg ctccaatact agctccagaa
    39061 agccctaatg ccattagacg agtaaagcta accaaatcac ttaagtaaga gctaatgcca
    39121 tataaattat ataatccact acctagacct aaaaggcttt tgctttttag taaagaaaca
    39181 accaaaatcc caaaggcatt gcccaatgca agccacttac caataaccga taagtaagca
    39241 aggccagata cattcttacc aagaataaat aacagcaggc ctaataaaat aaaagtccaa
    39301 gctagacttg acgtataggc cgtagcatat gctttcatcc ttacttgttg ccatgctcct
    39361 aacaaaagcc caaatataag tgtaacaaac ccaaatagca aagaaacaac caaaatagta
    39421 atcacatccg ttttagttga taataatgcc acaggtaagt caaaaccaaa gaaagaaccg
    39481 taaataagcc cccatagggc tactgaaata gctaaaatat taaagaaggt cactaaccgt
    39541 ttactggttt tttggagatt aaaaaagacc aatgcagcta gtgtcacagc atatagcaac
    39601 aaaccatagc ccaaatcagc taccatcatg ccaaaaaaag taaggtataa aggagctaaa
    39661 aaaggtgtcg gatctttttc ttgatatttt ggcaaagcgt acatttctgt aaccaattca
    39721 aacggtgcaa tgtagcggtg attacgtaac ttaatgggaa catcttccca atcgtctggc
    39781 gtgacatcgt aactatccag ataaaacata tcacctagtg tcttagtcat tagccctttt
    39841 aattggttaa ccgaatcagc ttctatccac ccttctaagg ctattagatg tcttgtaccg
    39901 agtaattgct ttttagtttg ctgccgttga tactgactaa ggacatagtc tatttcaacc
    39961 tgtaactgtg ccagtatctt ttgagactgc ctaagctctt ttagtagact atctttttca
    40021 gctagccaat tcgttagtaa ctctttgttt atacgaagtt gttctgatgg cagtagctgt
    40081 tctttatata ataagggttt aaactgatat ttctgtaaat gaatcgtatc ttgtgcttgc
    40141 caaaataaga caaggccgta ctcaaattct gtatggtata cttcctcaat aaaaagacct
    40201 ttgtgcttct ttaactgatg ataaaaatga tcatttgctg tactaggaat cgttccaact
    40261 cttgccgaca aaaaatgaaa ttgcgctaag tcctgaggca aaacagtcaa tgaacgccac
    40321 ttttctaact cctgacagta ttctgttagc tggtctattt tgttttgcag ctgctctagt
    40381 cgctctatct tttgtgacat cattttcaaa gccgtttgag ctgcttgttg tcgatggcgt
    40441 tcttcgaact gtttaaacga aagagatggc gttttttgtc tcaaggctgt taactttcct
    40501 attggtggta aaaacgctga taagctagca aggccgtttt ctaactcttg ttggctttgt
    40561 aaaaggtatt gtaaagcttc atcatctagt agaggttttt gatgcgtcag atcgtattgg
    40621 acgatttgtg gcaacttgac gttgcctttg ttaaaagcat cttgccaatg ttttagttgc
    40681 ttcatgtcac gaacttcaac taattgagat tgttgaagtg tttttaaaac aagatcaaga
    40741 taatcttttt caaaaaccat tgccagcttt ttcatttgac taatggccat atgtctccac
    40801 caccttatcc actaactccc taacaacggt tagcttatgc tcttctaaaa tggcatgaag
    40861 agctagctgt tgcgtcgcta acctcttttt tgaagctttt tgttcttgac gattagcttc
    40921 aaaaaatacc tcacgtttag cttctaatag tagctgagtt tcttgttcgt actcaagaaa
    40981 cagctgcttt aactcttctt cttttttgtc agctagagct tttttcttag catcgtaact
    41041 gtcataaatt gttctagcct cttgttcgat actattcatt agtgacaata gggtattcat
    41101 cacatatcaa ctccttatct ttatctattg ctaatttttt gactcactag gtacttaaaa
    41161 acaaagctac aagaaaggct acttttgggg gttaatcaac cagcgactat aagctagagc
    41221 taaatctcta acctatctac taacagcttt tttgagatag gataattgct tgattaagcg
    41281 aacagcatct ttaagttaag cgctttcaat tctatccata aaaacaatgg cttagttcag
    41341 aaaaaggtta gctaaaaacc tgtcaaagca gtaagatgac acagaactct aaagctttgt
    41401 attgctgtct ttcgtgtgtt atcttatact tctttaggca tttagtaacc ttgatctagt
    41461 tgacggtaag gaacctttac cagccaatag ccaagccaat gtgataacag tataactgct
    41521 atcttgttaa aagtcctgtc aaaaagatgt ttattagttt ctcgcttgta attcttgttt
    41581 tgttactaaa cggtaatttt taaagacaga acctccaata taacccacaa ttaagctgat
    41641 aataatacat ccaagggctg ttatcaacac tttagcgaca ggattatagg caaacatcac
    41701 cgcaaaaccg gcaatcggag tagctgtccc tggcgtatca ttaaccaatt tcattagggc
    41761 gatcaaaaca ccacatgcag caccaccaac aaaattagtc acataaatcg gaatgggatt
    41821 ggctgacgtc acatctgcct gcgttagtgg ttcaatagca aaagcaatat tgtcttttct
    41881 ttcacccaat tttaatcggt aaaaaagaac accattcata aaagaagagc caaagacaga
    41941 taaggcacct atagccatcg gaatacctgt cagaccaagc atggctgtca aagccattga
    42001 tgacagcgga gctgttgcta caacaacaat cgtaccgcct agtatcattc ccatgataat
    42061 agggttgcta ttagcacttg aggtcaggat gtctccaata gtcaataaag tactattgat
    42121 cactggcgta attaactgcg ccaagaaacg agttaatggt gcaccgacaa taataatcga
    42181 gattaaatca agcccaccag gaatattttt ttccatccac ttgattaaaa aagataccac
    42241 atacccagcc acaaaacctg ggataatacc tgttcctgaa actgacaaac caatcaaaac
    42301 ggcataacct ggagacacac caattgcaat ggcaaccaag atagcagcag ctacccctga
    42361 taatgagcca tttgctccgc ctacttcact tagaaactga aagcctaaaa ccttaccaaa
    42421 aaaagaatct tgaaaggctt caactaaaaa agaggcacaa gctgccgaag ccaaagctcc
    42481 cattgccttg gcaccatgag gagccttata attaaacaag ctaaaaatag ctaaaacaag
    42541 aatcaaaaga cttgttccaa taataatatc cataatctat tccaccaatc tatctcatta
    42601 cataagacac tataactgct tcactctggc aacagcttcg acttcaatca aagcttgcaa
    42661 aggaagcgcc gacacttgaa ccgctgtccg aacagggtag ggctcttcaa acaaatcagt
    42721 catgacatga tttaatattt ccacattggt cacatctgtt aaatacacat ttagcttata
    42781 gatatgattc aagtccaact tttgctctgc caaaatagat tgtaaattaa caaaaacctg
    42841 gcgacactgt gcctcaaaac catctgacaa ttgacctgtc actggattaa ggggtaattg
    42901 gcctgcggtg tatagaaaat gtccttcaat cgtataagtt gaataaggac ccattggttc
    42961 tgggtagctc ttcatatacg aacctcctca ttgataatat agttaaattt tacaggaaac
    43021 cgcattcaca gtcaataata aattctcttt ttgataaaaa attattatta aggtgattgg
    43081 caattgtttt ttagtctaaa atgtattact atatttgtaa ataagaagat agatatagga
    43141 agtttgacat ggataaagaa acgctaaact actggaaaac ggttatcaca tttcttcacg
    43201 atgtcttagg tgataactat gagattattt tacatgtgat tgataaaaat gatatctata
    43261 ttggagaact cgttaatagt catatcagtg gacggtctaa acagtctcct ctcaccacct
    43321 ttgctcttga tttgattacc aataaagtct ataaggaaaa agattttgtg accaattaca
    43381 aagcaatcgt tagtcctcag cacaaagaag ttcggggatc tacttttttt attaaagata
    43441 aaaagggtaa tctcgaggga atgctatgta ttaacttaga tatttcagcc tatcaaggag
    43501 tagctaggga cctcttaaaa cttgtcaatc tcaatttaga acatttcatc ccaactgcta
    43561 aagaacctaa aacagtcaca ccccagccag aagaagctgt tgaaatttta acctctaaca
    43621 tccaagatat tatcggtcaa attattgatc cctcgttact gagacacaat gtacatctta
    43681 gccaagatgt caaaatagac attgttgcca agctttatga aaaaggagta tttcaactaa
    43741 aaggagccgt ctcaaaagtc gctgatattc tatgtatttc cgaaccaagt gtttaccgct
    43801 accttaaaaa aattgaagcc gatcaataac caaatcacca taaaacgcca gttaaaagta
    43861 tttcagataa gcatcacaat ctctgatata ccgatgactg acgttttttt actcaatatg
    43921 cctttatggc aagaaaaggc tactagtcat taaccttcag ctatgcttag aggtacacct
    43981 gtcgcagctt gaacctcatc aaaagtaaac ccttcactga tttcaattac ttggatacca
    44041 tctggcgtta ctttaaacac acccatttct gtgataataa ggtcaaccac accttttgct
    44101 gttaaaggta aggtacattc ttttaggagc ttatgctttc ctttattggt atgctccata
    44161 gctacgatga cttttttagc accaactaat aaatccatcg ctcctcccat accaggaacc
    44221 attttgcctg gaatcaggta attggcgata tctccatttt ctgcaatttc taaagcaccc
    44281 aagacagtca agtcaacatg accaccacgt ataatgccaa aagaatctgc actattaaag
    44341 aaagcggctc ctggataaac cgtcacaggc tgtcctcctg cattaacgat agtcgaattc
    44401 ggatcatctg acttaggacc tagtccaata aacccatttt cagattgaaa atacactgtc
    44461 atttcttcag gcacaaaatt tgctactttg gttggtagac caatacctaa atttaccaaa
    44521 gtaccatctt ctaattcttt ggccacacgc tttgcaatac gattttgaat ctcttcttta
    44581 cttaatgcaa ccgccataac tatactcctt ctttaacaat gtaatctaca aaaatgtgtg
    44641 gtgtatgaac gcattctggt tgtatggtgc cgactggtac tatttctcgc acctgaacaa
    44701 ttgttgtttt tgcacaagct gccatcaatt gattaaaatt attttcagaa ccactatact
    44761 gtaaatttcc catttcatca gctttattag caaatatcaa ggtgacatct gcttcaaatg
    44821 ggtattctaa gagataggtt tttcccttga tggtttttgt ttccttgcct tctgccacta
    44881 aggttcctac ccctgttggc gtgtaaaagc caccaatgcc ataagctccg atgcgaattt
    44941 tttcggcgaa ggttccttga ggaatgagct caatcgtcgt ttcaccggca gtcatttggc
    45001 gcccagcttc tttatttaat ccaatatgtg ttgcataaat ggttttgaat tgatgattag
    45061 ccaccatttt tccaacaccg cgatcaacaa aaccagcatc attacagatc aaggttaaat
    45121 cttttgtgcc tttgtcaacc agtgcatcaa ttaacgcttc tggtgttcca ttagccataa
    45181 aaccaccgac cataatcgtg tcaccatctt taatatgact gatagcttct gctattgcta
    45241 ttcttttatt ctctttcata cggttacctt ctatcttgtt acgatgatac tttgaccttg
    45301 gccaccacca atacaaagcg ttgctaagcc atgtctcaca tcacgcttag ccatttctgc
    45361 taataaggtg actaaaatac gagcacctga tgctccaatc gggtgaccca aagcaatagc
    45421 tcctccattg acattaactt tttcattgtc aaaaccaagg tctcgtgaaa ccgctaaagc
    45481 ctgagcagca aaagcttcgt ttgcttcaat caaatcaatg tcatcaattg tcaactgagc
    45541 ctttgcaaga gcctttttcg tagcaggtat tggtccgcag cccataatac ttgggtctac
    45601 acctgcactt gcataactag tgattttagc taaaatagga agccctaatt cttcagcttt
    45661 ttctttactc atcaataaaa ttgctgctgc tccatcattg attcctgaag cattaccagc
    45721 agttactgtc ccctcatctt tgataaaagc agggcgtaac tttgctaact tatccactgt
    45781 cgttccaaat ttagggtatt catcttgatc aacgagtaaa gattcacctt tacgctgagg
    45841 aatagtgaca ggtgcaatct cttctttgaa acgacctttt tctatggcaa gttgggcttt
    45901 acgttggcta tcagctgcaa aggcatcttg ttcttctctg gtcagtccat actgttgcac
    45961 aatattttcg gctgttatac ccatgtgata acctgcaaaa gcatcactca acccatcttt
    46021 aagcatggta tctactactt tactatctcc catacgagat ccccaacgtt ggccttgtaa
    46081 aacgtaaggt gcttgactca tgttttcggt accaccgact aaaacaatat cagcgttgcc
    46141 cgtttgaatc ttttgagcac ctaactcaac tgcttttaag ccagaaccac agaccatatc
    46201 aatcgtaaaa gcaggggtat cataagaaag ccccgcattt agcgctactt gccgagcaac
    46261 attttgaccc agacctgcat gtaacacatt cccaaagatc acttcatcaa tggcatcact
    46321 tttaagaccg gtatcagcta aaatttttgt aacaactgtc acccccaaat ctacggctga
    46381 taaagattta aagactcctc caaaatttcc tatcggggta cgataagcag atgtgatcac
    46441 cacttcttta gtcatagaaa cttctccttt ttcattattg aaacgcttac atttttatta
    46501 tataatgtga actataataa acaaatatcg tttttttata tatgctataa ctaaacgtta
    46561 tcgaaagagg aaaatatgaa tatctcaagc ttggcttatt ttattgagat tgttaattgt
    46621 gactttaact taaccaaagc agcccaaaaa agtcatattt cgcaacccgc ccttagtagt
    46681 tatatcaaga ctatcgaaaa agaagaagga gtcgttttat tccagcgtca aaaagggcgt
    46741 ctagttggac taacctcagt cggcgaacgc ttttttgaaa atgctaaggt ggttatcgca
    46801 tcacatgagc aattaatggc tgatttgcgg agtgaatcca aaaaatacaa aggagaaatt
    46861 tcaatcggta ttcctcaact catctctggc attgtttttt ctgagctcat tccaaaactc
    46921 atcgaagaaa atcccgaagc taaatttcat attgtagaaa cgggtgctta tgaactgcaa
    46981 aaaaaactgt ttctaaaaga agtcgatttt gcgattttat taacacccac tacactagat
    47041 gctactattg ctgatatcga cattctccta agagatcaac tctgccttta tatgagtcac
    47101 aaaacctttc aagagacgtt tgatagtaat cctaacatct ctatcaaagc attacatcgt
    47161 caaaacttag taacttttga taaaaccttt atgattaatc atcagcttac caccttattt
    47221 gaagaagcaa atagtcttcc tgtagtcaaa tgttattctg cctcgtggga ttttttatta
    47281 aattgcacgc gctattcctc ttacctaaca atcctaccaa ggcctattac tcattttgct
    47341 catatggacg gtttagtgga ggtccaactg accgaacatc caaaatggga agttgttcta
    47401 gcctctctca agcacaataa aacaagccat ttaaaacatt atatcaagca caccatctta
    47461 gattatttca tgaaccagcg cttctaattg cttgactcag accttaaaaa aaaacgcctg
    47521 aaggaaacaa tcccaagcgt ttcatgtagt tatttcctaa aatacaaata ggtaaatgat
    47581 ggcaatacct gtgaccacca agacacaagg aatagtataa gccacaattt gattgaattt
    47641 tagtttgaag tttgagagat aagataaggc ccaaaaagga actatcaagt ttgttgtggc
    47701 atctcctaat tgataagcct gcaaaatctt acctagatca ttaccgactt cgatagttgc
    47761 agggacgata tatggagctt caatcacaaa ttttgagcct cctgatggta ccgcaatatt
    47821 cagcaaagcc gagtaaagat aagcaaagac cggccatgtt gtcccatttg aaatagcgac
    47881 aaagaaatgg gaaatctcta gtcctagacc tgtactttga atgattccaa aaataccagc
    47941 gtaaaatgga aactgaagca ccaagcccca agatgacatg accccatctt tgaataagga
    48001 accataatat tctggtcctt gttgaccaca aagaagcaac cctaagccta aaaataaaaa
    48061 gttaaaacta ttgagatcta gaccagacaa gcccaccacc gaaaaatgat aaaggctata
    48121 gctaacaatg gccccaccaa caagatacat cactaagcga gatgcgttca tcttttcagc
    48181 aggtgtgttt cttgcaataa caacatgtga agcggtatca agactatggt gatagatctc
    48241 atcatcaagt tccatgattt tagtttcatc ttttgggtgc gccaataaag caaagcaaag
    48301 cggtaccagc atacacacca ctaaaagact gataaatgga agattcaaaa ctgattctgt
    48361 cagaggaaca ctttcaggaa caacctgttt ataagcatct gcgacactat ttcgcaaata
    48421 atctggagta gccgaataaa gcaccgcagc accagataaa ccagaagtag ctggtaaaaa
    48481 ggtaaaaaat aaagtagcaa caaacaaagg tgtatgcacc ttaatccctt tttgcctagc
    48541 ctgaacaagc aactctttcc caaacacaat ggcaaccatc ataccaagac cccagtgtaa
    48601 aaagccaaag atagaaccta ctacaataga aaaaataaaa atctgagtcc tggtttgagg
    48661 cagtttggca agggacttaa acattttatg agcaggtgga gaactcgcta cagcattccc
    48721 tgtcgcaagt aaaatagtca tctgcatggc aaaggacaac agcccccaaa aaccatttac
    48781 ccagccatag ataatcccgc ccgtcttttc cgtaccaata aagggactat ccgtcatcaa
    48841 cacagacata gtaacgacta aaaaggttaa aataaaacag ataaaaagcg attctggcat
    48901 ccatttcata aagccatcaa tatagcgatc cataaacctt tttttggttt tgatgttatt
    48961 catgttgtca attcctttca cattgtccac aagcactttt gttcttttga tcagtaacct
    49021 atcgataata ctaataagcg tttctttagt tcatttagtt atcaataagt ttaaacccac
    49081 aaaaaacact tttgttccct ttttattatg acaacgtttt catagacaaa caactatgat
    49141 ttttttattg cttccataac ccttatttat agcaaaaaga a
//
LOCUS       DATXQK010000011        41064 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.011, whole genome shotgun sequence.
ACCESSION   DATXQK010000011 DATXQK010000000
VERSION     DATXQK010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 41064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41064
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.011"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            124..285
                     /gene="sagA"
                     /locus_tag="VC394_000646"
     CDS             124..285
                     /gene="sagA"
                     /locus_tag="VC394_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOMM family cytolysin streptolysin S"
                     /protein_id="HEP5994138.1"
                     /translation="MLKFTSNILATSVAETTQVAPGGCCCCCTTCCFSIATGSGNSQG
                     GSGSYTPGK"
     gene            507..1457
                     /gene="sagB"
                     /locus_tag="VC394_000647"
     CDS             507..1457
                     /gene="sagB"
                     /locus_tag="VC394_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptolysin S biosynthesis dehydrogenase SagB"
                     /protein_id="HEP5994139.1"
                     /translation="MSFFTKEQQPKENCPPITVEKARQLFEFNTNHLSLSDYHHQTVL
                     KTSKQLVAQHLMPNATDNLSQHFLMNYKANNNYLGFQASIVDFFTDSAVANFSSSYVY
                     ESQEKIIRLPKPTKISTALSTCIIKRRSHRQFSDRQMPLQDLSNILYYACGVSSQASI
                     RDGASDKITLRNCASGGGLYPIHLVFYARNISKLIDGFYEYLPYQHALRCYRHSSEEN
                     VRDFAEYGAINAENCNIIIIYVYHYMKNTRKYGNQATAYAFIESGEIAQNIQLTATAL
                     TYGSIDIGGYNKEYLQELLDLDGLGEHVIHMTLVGTKESQ"
     gene            1454..2512
                     /locus_tag="VC394_000648"
     CDS             1454..2512
                     /locus_tag="VC394_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptolysin associated protein SagC"
                     /protein_id="HEP5994140.1"
                     /translation="MKYQLNSNVRVVTFQDTFCFRKGIWDFNEAILDLTQEPQNLKIV
                     YQEIVSQLVKGVAIDTETYENTLEPEMFANLMEVISGLYYNDMLMLEDDYNLEENVMK
                     ILMGNFRFMAQAGHMVSNAPVLFISDSTYVNESAELLAEQLHLQLQVASDDLKMLIQQ
                     TDVSSRLDALEHRRNMNCLSEALCDYQSIIICQERLNIMMLRHLNEVSVAMKKQLVIG
                     FVDGPFLHTCTLNPPHSADFDSLERRVLARLQDSTLYQHFANQILPATQDVSQAYLPL
                     LNVLMNLVVSEAFIIAQTGSSKFEGRLLSIYLPTLEIQVQDILKMSNSQTQGALAKLK
                     YEDQQISTREIVKKLLDE"
     gene            2532..3890
                     /locus_tag="VC394_000649"
     CDS             2532..3890
                     /locus_tag="VC394_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HEP5994141.1"
                     /translation="MLYYYPSFNHIFDELKSLSGNRTGILNQSQVPVCNHPHDVYLKS
                     ITGQMPDYHKQFIGELSQVSYHIIGYGSYYEEALIKYLGESIERYATVIAGDLLSDRI
                     VYASYNELKLLHKVMPLEYLQVFTQEQIALSCDLQMMMCDKMVTENDVLGWVKCPMFF
                     EDAEMYVPAQMLCVGYKTNETVGERRIIPGFSTGTASHKTLEAAMCNSLIEYIQIDSM
                     MLSWYTKKPCPKIIVDDPDIEVILEEARLGKDSLYDIIPIDMTVGEDNPLYTFGIILK
                     NKYDEGPYLLFGVQAGLDPKHALLRGIMEASAISYSYYYNLLYQKASLANIECEEPLF
                     LDLDSNVFYYAHPKDQDHKWKAFEPLISGEVLLSDLEDHSGKDKKEDLKTLLAYAKKV
                     SPNAVFLDITPPEALEKGWYVTRVLMPELLEMCIPAFPFANHPRMRQFGGVTNAFVHP
                     MP"
     gene            3865..4536
                     /locus_tag="VC394_000650"
     CDS             3865..4536
                     /locus_tag="VC394_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEP5994142.1"
                     /translation="MPLSIQCLNLCFLLVTFCPSIPMQAIFGKEDSGYAFNLIGFLRA
                     TLIYDILALVSIYVLSPQITLSLESIDSKTFFMGLVFCVLIVLIELVFLHGLRCWQKK
                     QWLPATFSFVGTTNDWSKIGYPLLLALFEETIYRFLWFNILAFQWHLPTIIVLIVTSF
                     CYALNHLLMGKSIFYAKLVTGIIYGSIYMLTSQLWLVVIMHVGGNLLVECLSHLQTKK
                     KKGVT"
     gene            4533..5216
                     /locus_tag="VC394_000651"
     CDS             4533..5216
                     /locus_tag="VC394_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptolysin associated protein SagF"
                     /protein_id="HEP5994143.1"
                     /translation="MMLLVLLSFLGLTTLWLTSYRRRCLARWHLQWLVALSYQDFLDV
                     LLSLFQFVIIILVLFFYSATINLGEVLTFLTQTSWHWQILCYLVLYLMAIIEMTLLVL
                     ILIFDVLLQKDSRLLFKKITWLPFRQDKPVLSILLLGIAILVDSLFYLGLLILLGEQS
                     LTSLATLILGYGIVKACRYSGWLNVLLAFCLFTIVGLWAVTATLLYGWLVGAMILTVT
                     YLMISCKEY"
     gene            5239..6162
                     /locus_tag="VC394_000652"
     CDS             5239..6162
                     /locus_tag="VC394_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5994144.1"
                     /translation="MSFVQLTNVVKSYKNGKKAVNDVSLSIEAGNIYGLLGPNGAGKS
                     TLINLILGLIPLSSGKITVLGQSQKTIRKISSQIGYVPQDIAVYPDLTAYENVELFGS
                     LYGLKGAQLKKQVLKSLEFVGLHSQAKQFPSQFSGGMKRRLNIACALVHSPKLIIFDE
                     PTVGIDPQSRNHILESIRLLNKEGATVIYTTHYMEEVEALCDYIFIMDHGQVIEEGPK
                     FELEKRYVANLANQIIVTLTDSRHLELADKPDWSLIEDGEKLMLKIDNSDMTSVVHQL
                     TQANITFSEIRHNHLNLEEIFLHLTGKKLRD"
     gene            6171..7298
                     /locus_tag="VC394_000653"
     CDS             6171..7298
                     /locus_tag="VC394_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP5994145.1"
                     /translation="MVLFHLIKKESLQIFRNRTALLMMVIFPILMIVILSFAFKSSFN
                     TATTVPKLTIRYQLEGEKTDYQKNFLAFLKVLNQKLHLEAKPSNSLEKDRQRVSEGAL
                     TAVLEVKKNQTIKVITNNINQQNADLINMLVKNYVDNAKTYDSIAALYPQQLNHIRKR
                     SVDYVKVSSIQTSKGMTSADYYAISMFTMITFYSMMSAMNLVLSDRQQRITNRIHLTG
                     VSPSLLVFGKLIGAMLATTVQLSLLYIFTRFVLRVNWGTNEWMLIGITASLVYLSVAI
                     GIGLGISIKNEAFLTVASNTIIPIFAFLGGSYVPLTTLHSSIINQLSNISPIKWVNDS
                     LFYLIFGGQYNPIPVTLIVNISIGTIFIILALIGMRKQVTT"
     gene            7295..8413
                     /locus_tag="VC394_000654"
     CDS             7295..8413
                     /locus_tag="VC394_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP5994146.1"
                     /translation="MICFIKTLFVKIKRKKTSYVTFFLMPILTTLLALSLSFSNNNQA
                     KIGILDKDNSQISKQFIAQLKQNKKYDIFTKIKKEHIDHYLQDKSLEAVLTIDKGFSD
                     KVLQGKSQKLNIRSIANSEITEWVKAQTNYLLENYNIIGDVALGNEDTFNRILQKNQQ
                     LNYDVKQVTLTDRSRSKAVSSTTTGFLLILMLGSTSVIYSGILADKSGQLYHRLMLSN
                     LSRFRYMLSYVCVGFVAFTIQIVIMLSLLKVFNISFFVPTSLLLIIFFLFSLLAIGFG
                     LLIGAITQNSQQSSQLANLIVMPTSMLAGCLWPLSITPSYMQAIGKLLPQNWVLSAIA
                     IFQSGGTLSQAWPYLLALMGTALALISFSSLLLKPTKL"
     gene            8985..11717
                     /locus_tag="VC394_000655"
     CDS             8985..11717
                     /locus_tag="VC394_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpnA family nuclease"
                     /protein_id="HEP5994147.1"
                     /translation="MINKKYIIPVSLLTLAITLTSVEEVTSRQNLTYANEIVTQRPKR
                     ESVISDKSNFPVISPYLASVDFGERKTSLPTPDKGVKVTTEQSIAQVRKGPEERTYTV
                     TGKITSVINGWGGYGFYIQDSEGIGLYVYPQKDLGYSKGDIVQLTGTLTRFKGDLQLQ
                     QVTAHKKLELSFPTSVKEAVISELETTTPSTLVKLSHVTVGELSTDQYNNTSFLVRDD
                     SGKSIVVHIDHRTGVKGADVVTKISQGDLINLTAILSIVDGQLQLRPFSLEQLEVVKK
                     VTSSNSDASSRNIVKIGEIQGASHTSPLLKKAVTVEQVVVTYLDDSTHFYVQDLNGDG
                     DLATSDGIRVFAKNAKVQVGDVLTISGEVEEFFGRGYEERKQTDLTITQIVAKAVIKT
                     GTAQVPSPLVLGKDRIAPANIIDNDGLRVFDPEEDAIDYWESMEGMLVAVDDAKILGP
                     MKNKEIYVLPGSSTRPLNNSGGVLLPANSYNTDVIPVLFKKGKQIIKAGDSYKGRLAG
                     PVSYSYGNYKVFVDDSKNMPCLMDGHLKPEKTNLQKDLSKLSIASYNIENFSANPSST
                     KDEKVKRIAESFIHDLNAPDIIGLIEVQDNNGPTDDGTTDATQSAQRLIDAIKKLGGP
                     TYRYVDIAPENNVDGGQPGGNIRTGFLYQPERVSLSDKPKGGARDALTWVNGELNLSV
                     GRIDPTNAAWKDVRKSLAAEFIFQGRKVVVVANHLNSKRGDNALYGRVQPVTFKSEQR
                     RHVLANMLAQFAKEGAKHQANIVMLGDFNDFEFTKTIQLIEEGDMVNLVNRHDISDRY
                     SYFHQGNNQTLDNILVSRHLLDHYEFDMVHVNSPFMEAHGRASDHDPLLLQLSFSKEN
                     DKAESSKQSVKAKKTSKGKLLPKTGDSLVYVITLLGTASLLVPILLLTKGKKES"
     regulatory      11882..11997
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            11995..12495
                     /locus_tag="VC394_000656"
     CDS             11995..12495
                     /locus_tag="VC394_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEP5994148.1"
                     /translation="MTKLTVHDYVHIGLVAALYVVLTITPPLNAISYGMYQFRISEMM
                     NFLAFYHRKYIIAVTLGCMIANFYSFGLIDVFVGGGSTLIFVTLGVILFSKYQKDYLF
                     NGIFNKAFVYFSFFFATSMFTVAIELYFFGAPFLLTWFTTALGELVSLLIGSLIIDKL
                     SQRISF"
     gene            12689..14647
                     /gene="ligA"
                     /locus_tag="VC394_000657"
     CDS             12689..14647
                     /gene="ligA"
                     /locus_tag="VC394_000657"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEP5994149.1"
                     /translation="MKKRIKELTDLLNRYRYDYYTKDAPSVSDSDYDKLYRELVTLEQ
                     SYPEYVLQDSPTQQVGGTILKGFEKYRHQYPLFSLQDAFSREELDAFDKRVKAEFPNA
                     TYLAELKIDGLSISLSYENGFLQVGATRGDGNIGENITENIKKIKDIPHQLSEPLTIT
                     VRGEAYMSRQSFKAINEARQENGETEFANPRNAAAGTLRQLDTAVVAKRQLATFLYQE
                     ASPTARNQQNEVLAELADLGFSVNPYYQLTSSMDEIWNFIKTIEAKRDQLAYDIDGVV
                     IKVNSLAMQEELGFTVKAPRWAIAYKFPAEEKEAEILSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRIGDTVIVYKAGDIIPAVLNVVMSKRNQQEVMLIP
                     KLCPSCGSELVHFEDEVALRCINPLCPSLIQRSLEHFASRDAMNITGLGPAIVEKLFL
                     AGFVHDVADIYQLTKEDFMQLDGIKEKSADKLLAAIEASKSNSAEKLLFGLGIRHIGS
                     KVSRLILEVYGDISALLTAKEEEIARIDGLGSTIAQSLTQYFEQKTAAILVDELKTAG
                     VNMHYSGQKVNSDAALFGLTVVLTGKLNQLNRNEAKDKLEALGAKVTGSVSKKTDLVI
                     AGSDAGSKLEKAKSLGIRIEDEDWLRQL"
     gene            14661..15683
                     /locus_tag="VC394_000658"
     CDS             14661..15683
                     /locus_tag="VC394_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEP5994150.1"
                     /translation="MKKQLRARLIYNPTSGQELMRKSVPEVLDILEGFGYETSAFQTT
                     AQKNSALNEARRAAKAGFDLLIAAGGDGTINEVVNGIAPLKKRPKMAIIPTGTTNDFA
                     RALKVPRGNPSQAAKLIGKNQTIQMDIGRAKKDTYFINIAAAGSLTELTYSVPSQLKT
                     MFGYLAYLAKGVELLPRVSNVPVKITHDKGVFEGQVSMIFAAITNSVGGFEMIAPDAK
                     LDDGMFTLILIKTANLFEIVHLLRLILDGGKHITDRRVEYIKTSKIVIEPQCGKRMMI
                     NLDGEYGGDAPITLENLKNHITFFADTDLISDDALVLDQDELEIEEIVKKFAHEVEDL
                     EQELEE"
     gene            16075..16272
                     /locus_tag="VC394_000659"
     CDS             16075..16272
                     /locus_tag="VC394_000659"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEP5994151.1"
                     /translation="MNPIFALALACFGVSLAEGFLMANLFKAASRQPEIIGQLRSLMI
                     LGVAFIEGTFFVTLVMAFILK"
     gene            16307..17023
                     /gene="atpB"
                     /locus_tag="VC394_000660"
     CDS             16307..17023
                     /gene="atpB"
                     /locus_tag="VC394_000660"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEP5994152.1"
                     /translation="MEEAKIPMLKLGPITFNLTLLAVCIVTIAVIFAFVFWASRQMKL
                     KPEGKQTALEYLISFVDGIGEEHLDHNLQKSYSLLLFTIFLFVAVANNLGLFTKLETV
                     NGYNLWTSPTANLAFDLALSLFITLMVHIEGVRRRGLVAHLKRLATPWPMTPMNLLEE
                     FTNFLSLAIRLFGNIFAGEVVTGLIVQLANYRVYWWPIAFLVNMAWTAFSVFISCIQA
                     FVFTKLTATYLGKKVNESEE"
     gene            17041..17535
                     /gene="atpF"
                     /locus_tag="VC394_000661"
     CDS             17041..17535
                     /gene="atpF"
                     /locus_tag="VC394_000661"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEP5994153.1"
                     /translation="MSITFGELVGNFILVTGSVIVLLLLIKKFAWGAIESILQTRSQQ
                     ISRDIDQAEQSRLSAQQLEAKSQANLDASRSQASKIISDAKEIGQLQGDKLVAEATDE
                     AKRLKEKALTDIEQSKSDAISAVKTEMSDLTVLLAEKIMGANLDKTAQSQLIDSYLDD
                     LGEA"
     gene            17535..18071
                     /locus_tag="VC394_000662"
     CDS             17535..18071
                     /locus_tag="VC394_000662"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEP5994154.1"
                     /translation="MTKKEQALIEQYAKSLVEVASEHHSLDALQADVLAILETFVTTN
                     LDQSLSSLAVPHAEKIKLLTLLKGNNSVYMNNFLNLILQNEREAYLYQMLQTVLNEIA
                     IVSNQYDVTVTSSLPLTEEQKSRVRAVVAKKFAVTAGRLIEKVDPSLIGGFIISVNNK
                     VIDTSIRRQLQAFKMNLK"
     gene            18087..19595
                     /locus_tag="VC394_000663"
     CDS             18087..19595
                     /locus_tag="VC394_000663"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEP5994155.1"
                     /translation="MAINAQEISALIKKQIENFQPNFDVTETGIVTYIGDGIARARGL
                     DNAMSGELLEFENGAYGMAQNLESNDVGIIILGDFSAIREGDVVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPVDGLGDIETTGFRPVETPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTSVAIDAILNQKGQDMICIYVAIGQKESTVRTQVETLRRYGAL
                     DYTIVVTASASQPSPLLFIAPYAGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERSAKVSDDLGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLQENLFNSGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQPLHKPLPVEKQVVILYALTHGFLDDVPV
                     DDILAFEEALYDYFDVHYNDLFETIRTTKDLPEEAALDAAIKAFKEHSNFKS"
     gene            19611..20486
                     /locus_tag="VC394_000664"
     CDS             19611..20486
                     /locus_tag="VC394_000664"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEP5994156.1"
                     /translation="MAGSLSEIKAKIISTEKTSKITSAMRMVSSAKLVKSEQAARDFQ
                     IYASKIRQITTDLLKSELTIGSDNPMLVSRPVKKTGYIVITSDKGLVGGYNSKILKSV
                     MDMITEYHADGDYEIISIGSVGSDFFKARGMNVAFELRGLADQPSFEQVRQIISQSVD
                     MFVNEIFDELYVCYNHHVNSLTSQVRVQQMLPISDLVADEAAEEGVTGFELEPNRHDI
                     LDQLLPQFTESLIYGAIIDAKTAEHAAGMTAMQTATDNAKNVINDLTIQYNRARQAAI
                     TQEITEIVAGANALE"
     gene            20648..22054
                     /gene="atpD"
                     /locus_tag="VC394_000665"
     CDS             20648..22054
                     /gene="atpD"
                     /locus_tag="VC394_000665"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEP5994157.1"
                     /translation="MSSGKIAQVVGPVVDVMFASGDKLPEINNALIVYKDSDKKQKIV
                     LEVALELGDGMVRTIAMESTDGLTRGLEVLDTGRAISVPVGKETLGRVFNVLGETIDL
                     EEPFAEDVDRQPIHKKAPSFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTQKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLTQ
                     MGIYPAVDPLASSSRALSPEIVGEEHYAVATEVQRVLQRYRELQDIIAILGMDELSDE
                     EKTLVGRARRIQFFLSQNFNVAEQFTGLPGSYVPVAETVRGFKEILEGKYDDLPEDAF
                     RSVGPIEDVIKKAEKMGF"
     gene            22067..22483
                     /locus_tag="VC394_000666"
     CDS             22067..22483
                     /locus_tag="VC394_000666"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEP5994158.1"
                     /translation="MTQMTVQVVTPDGIKYDHHAKCISVTTPDGEMGILPNHINLIAP
                     LQVHEMKIRRGGEDEKVDWIAINGGIIEIKDNVVTVVADSAERDRDIDVSRAERAKLR
                     AEREIAQAETTHNIDEVRRAKVALRRALNRINVSKK"
     gene            22749..23006
                     /locus_tag="VC394_000667"
     CDS             22749..23006
                     /locus_tag="VC394_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEP5994159.1"
                     /translation="MVCYNNPMELLMKIIAFSCHLFFIGLSYQLLISVIDWTKFSHYH
                     PENLGRLRLFVFLLATALGYLVSHCMLELLQISQTLFFEFR"
     gene            23071..24342
                     /gene="murA"
                     /locus_tag="VC394_000668"
     CDS             23071..24342
                     /gene="murA"
                     /locus_tag="VC394_000668"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEP5994160.1"
                     /translation="MDKIIIEGGQTRLEGEVVIEGAKNAVLPLLAASILPSKGKTILR
                     NVPILSDVFTMNNVVRGLDIRVDFNEAANEITVDASGHILDEAPYEYVSQMRASIVVL
                     GPILARNGHAKVSMPGGCTIGSRPINLHLKGLEAMGATITQKGGDITAQADRLQGAMI
                     YMDFPSVGATQNLMMAATLADGVTTIENAAREPEIVDLAQFLNKMGARIRGAGTETLT
                     ITGVTSLHGVEHDVVQDRIEAGTFMVAAAMTSGNVLIRDAVWEHNRPLISKLMEMGVS
                     VTEEEYGIRVQANTPKLKPVTVKTLPHPGFPTDMQAQFTALMAVVNGESTMVETVFEN
                     RFQHLEEMRRMGLQSEILRETAMIHGGRQLQGAPVMSTDLRASAALILTGIVAQGVTI
                     VNNLVHLDRGYYQFHEKLAKLGATISRSSEV"
     gene            24346..24534
                     /locus_tag="VC394_000669"
     CDS             24346..24534
                     /locus_tag="VC394_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEP5994161.1"
                     /translation="MTNGLKYVLEQMLLLLVIAALACLFLAIGLMIGYSFMGDGHSPW
                     HILSMDKWAELVNKFTGK"
     gene            24570..25434
                     /locus_tag="VC394_000670"
                     /pseudo
     CDS             24570..25434
                     /locus_tag="VC394_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
     gene            25717..26760
                     /gene="pheS"
                     /locus_tag="VC394_000671"
     CDS             25717..26760
                     /gene="pheS"
                     /locus_tag="VC394_000671"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019314825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEP5994162.1"
                     /translation="MDLQAQLEELKTKTLETLQSLTGNHTKELQDLRVAVLGKKGSLT
                     ELLKGLKDLSNDLRPVVGKQVNEVRDLLTKAFEEQAKIVEAAKIQAQLDAESIDVTLP
                     GRQMTLGHRHVLTQTSEEIEDIFLGMGFQIVDGFEVEKDYYNFERMNLPKDHPARDMQ
                     DTFYITEEILLRTHTSPVQARTLDQHDFSKGPLKMVSPGRVFRRDTDDATHSHQFHQI
                     EGLVVGKNISMGDLKGTLEMIIKKMFGEERSIRLRPSYFPFTEPSVEVDVSCFKCGGK
                     GCNVCKKTGWIEILGAGMVHPSVLEMSGVDAKEYSGFAFGLGQERIAMLRYGINDIRG
                     FYQGDQRFSEQFN"
     gene            26970..29375
                     /locus_tag="VC394_000672"
     CDS             26970..29375
                     /locus_tag="VC394_000672"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEP5994163.1"
                     /translation="MLVSYKWLKELVDIDVTPAALAEKMSATSIEVEGIEVPADGLSK
                     LVVGHVLSCEDVPETHLHLCQVDTGDETPRQIVCGAPNVKAGIKVIVAVPGARIADNY
                     KIKKGKIRGMESLGMICSLQELGLSDSIIPKEFSDGIQILPEEAVPGDAIFKYLDLDD
                     HIIELSITPNRADALSMRGVAHEVAAIYGKSVSFPQKNLQESDKATSEAIEVAIASDN
                     VLTYASRVVENVKVKPSPQWLQNLLMNAGIRPINNVVDVTNYVLLYFGQPMHAFDYDK
                     FEDHKIVARAARQGESLVTLDGEKRDLTTEDLVITVADKPVALAGVMGGQATEIDANS
                     QTVVLEVAVFDGKSIRKTSGRLNLRSESSSRFEKGVNYATVLEALDFAAAMLQELAEG
                     QVLSGHVQAGQLPTEPVEVSTSLDYVNVRLGTELTFADIQRIFDQLGFGLTGDETSFT
                     VAVPRRRWDVSIPADLVEEIARIYGYDKLPTTLPEAGGTAAELTPTQALRRKVRGLAE
                     GLGLTEIISYALTTPEKAVEFAVAPSHLTELMWPMSVERSALRQNMVSGMLDTVAYNV
                     ARKQSNLALYEIGKIFEQEANPKEDLPNELNHFAFAICGLVAQKDFQTQAQAVDFYHA
                     KGILDTLFANLNLKVQYVPTKDLASMHPGRTALILLDEQVIGFVGQVHPGTAKAYSIP
                     ETYVAELDMAALEAALPSDQTFAEITKFPAMTRDVALLLDREVSHQAIVTAIESAGVK
                     RLTKIKLFDVYEGVTIQAGKKSMAYSLTFQNPNDNLTDEEVAKYMEKITKALTEQVGA
                     EVR"
     gene            29485..29862
                     /locus_tag="VC394_000673"
     CDS             29485..29862
                     /locus_tag="VC394_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEP5994164.1"
                     /translation="MYQTKITGNRLYHTVSKGYGDSVTLFGKTDQGETPMSLLVIALS
                     SCVTMCVQGFYHRQFQQDTLAVSVEASYEEEVFELLVRIDDVLDDKKKADLLAYIGTY
                     CRVKQLLKPEVMVTITLEGLSHV"
     gene            29855..30241
                     /locus_tag="VC394_000674"
     CDS             29855..30241
                     /locus_tag="VC394_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HEP5994165.1"
                     /translation="MSDAGLKTYQKQWAYQKYWVMAHSQQHYNALRELFKGNQWSEEK
                     VLTFHCLIEEAQAIPPTVKSLRTAYQHVWGYFKKVASQEEKDHFKDLDAQLETKSEEM
                     LCFLQEMTAHYQPSYLLSCRLITKGP"
     gene            30314..31390
                     /locus_tag="VC394_000675"
     CDS             30314..31390
                     /locus_tag="VC394_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP5994166.1"
                     /translation="MFLALNEMKQSKLRYGLIAGLLCLVAYLMFFLSGLAFGLMQENR
                     SAVDLWKADSILLAKDADATLTLSQVSRAQENQITADKVAPLAQLNTVAWSVKNPKDA
                     DKVKVSLFGIDSNSFIRPNIVKGRLFKTNKEVVLDQSLAKEEAFAIGKDFYTSSSSQA
                     LTIVGYTQNARFSVAPVVYMNLEAFETLKYGEPLPKDKQVVNAFITKGSLTDYPKKDF
                     QKLDIKTFITKLPGYSAQLLTFGFMISFLVIISAIIIGIFMYILTIQKAPIFGIMKAQ
                     GISNKTITTAVLMQTFFLSFLGSGLGLLGTWLTSLLLPTVVPFQSNWFLYLAIFVSMI
                     CFALLGTLFSVFNIIRIDPLKAIG"
     gene            31400..32068
                     /locus_tag="VC394_000676"
     CDS             31400..32068
                     /locus_tag="VC394_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5994167.1"
                     /translation="MSVLTFKQVTKTFQDGHHEINALKATDFSIEAGEFVAIIGPSGS
                     GKSTFLTIAGGLQTPSSGQLIIDGTDYTHLSEKERSRLRFKSVGFILQASNLIPFLTV
                     QQQLELVDHLTGSKEKAKANQLFDDLGITGLKHQLPQELSGGERQRAAIARALYHDPA
                     LILADEPTASLDTEKAYEVVKLLAKESKEKNKAIIMVTHDDRMLKYCDKVYRMQDGEL
                     RQER"
     gene            complement(32173..33090)
                     /locus_tag="VC394_000677"
     CDS             complement(32173..33090)
                     /locus_tag="VC394_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEP5994168.1"
                     /translation="MKTDDLRESQQVEDRRGQSSGSFGGGGLGGGLLLQLLFSRGGWK
                     TKLVILLLLLVMGGGGLSSVLGGKPSSTNNNAYQSSQVTRTNGDKASQEQVSFVSKVF
                     ASTEDYWTKTFREKGLTYHKPTLVLYTGATQTACGRGQASSGPFYCPGDQKVYLDISF
                     YNELSTKYGAKGDFAMAYVIAHEVGHHIQNELGIMDNYASARQGKSKAKANQLNVKLE
                     LQADYYAGAWANYVQGQGLLEKGDIEEAMAAAHAVGDDTLQEETYGRTVPDSFTHGTS
                     KQRQRWFDRGYQYGDFEHGDTFSIPYSKL"
     gene            33241..36456
                     /gene="rexB"
                     /locus_tag="VC394_000678"
     CDS             33241..36456
                     /gene="rexB"
                     /locus_tag="VC394_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEP5994169.1"
                     /translation="MKLIYTEMSYSMTEILVNEARKAADQGYRVFYIAPNSLSFEKER
                     EVLTLLPERGTFSIIVTRFVQMSRYFTVESSPSKQHLDDTTLAMIFYRALMQLKPEDL
                     PSYGRLQNNSVFIEQLVELYKELKNAQLSVHDLTGLDHPQKQEDLIKIIELAETIMIQ
                     QDYNQDSPLQSFARAIKLGLLNNQLSKTVIVIDGFSRFSAEEDYLLSLLNNNCQEVII
                     GSYVSQKAYQKSFIKGNIYEASLHFLQDLAQKYHIKPVFATSNQVFKPAFSRLTQLFE
                     ATHDFSQVDWQLQKNDLDHFSLWQCHHQKEEIEHVAKSIRQKLYEGYRYKDILVLLGD
                     MDAYQLQIGPIFDKFEIPYYLGKAEPMAAHPLVQFIESLERSQRYNWRREDILNMLKS
                     GLFGCFDDSDIDRFEEYTQFADIKGFTKFSKPFTINSSRQYPLDFLNEMRQDIVLPLQ
                     ELFKSQKQLGASLVDKLILFFKKIRLAENMQGLAQSQLEVEKNEEVWKRFTDILTSFH
                     HIFGQEKLRLSDCLALIKTGMKSAQYRVVPATLDVVTIKSYDLVQPHSKPFVYAIGLT
                     QSHFPKQIHHSGLLSDQERARINEIRNYRHFDIASAENSKKNHQTALSLFNAATKELV
                     LSVPTVINETFDDLSPYLKELISFGLPLLDKGKNYLSYDNSDIGNYKALLSQIIAINR
                     QDLIEMSDQDKMFWTVVLRYLRKQLRKQQLELPTSDYRLSTKPLSKEVIEVCFPKGIP
                     LKLSATALTVFYNNQYNYFLKYVLNLNKTESIHPDSRIHGQYLHRVFERLMKDHTQEP
                     FDNKLKQAIYHTNQESFFQQVYQDNAEAEYSLAILEDIVRSTAPILQLNQNIKVIDQE
                     KNFHLDMGNEILVHGIIDRIDQLSDGSLGIVDYKSSANQFDIGTFYNGLSPQLVTYLA
                     ALKQIAPHDINQLFGAMYLHLQDPKLDLVTFKQIDNTLVESIYKALTYKGIFSEVEKE
                     HLSTGAYQTKNALYSNDELETLLNYNKYLYLKAAKHIKKGHFLINPYTSDGKTVQGDQ
                     LKAITRFEADLDMAQARRLVTLPAKEKKECFLTLMRKESHL"
     gene            36417..40085
                     /gene="addA"
                     /locus_tag="VC394_000679"
     CDS             36417..40085
                     /gene="addA"
                     /locus_tag="VC394_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020905049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEP5994170.1"
                     /translation="MLFNINEKGEPLVISFAPFLSPEAIKHLQENERCSDQSQKRTAQ
                     QIEAIYTSGQNILVSASAGSGKTFVMVERILDKILRGVSIDRLFISTFTVKAATELRE
                     RIENKLYSQIAQTTDFQMKVYLTEQLQSLGQADIGTMDAFAQKVVSRYGYSIGISSQF
                     RIMQDKAEQDVLKQEVFSKLFSEFMNQKEAPVFRALVKNFSGNCKDTSAFRELVYTCY
                     SFSQSTENPKIWLQENFLSAAKTYQRLEDIPDHDIELLLLAMQDTANQLRDVTDMEDY
                     GQLTKAGSRSAKYTKHLTIIEKLSDWVRDFKCLYGKAGLDRLIRDVTGLIPSGNDVTV
                     SKVKYPVFKTLHQKLKQFRHLETILMYQKDCFPLLEQLQDFVLAFSEAYLAVKIQESA
                     FEFSDITHFAIKILEENTDIRQFYQQHYHEVMVDEYQDNNHMQERLLTLLSNGHNRFM
                     VGDIKQSIYRFRQADPQIFNQKFRDYQKKPEQGKVILLKENFRSQSEVLNVSNAVFSH
                     LMDESVGDVLYDEQHQLIAGSHAQTVPYLDRRAQLLLYNSDKDDGNAPSDSEGISFSE
                     VTIVAKEIIKLHNDKGVPFEDITLLVSSRTRNDIISHTFNQYSIPIVTDGGQQNYLKS
                     VEVMVMLDTLRTINNPRNDYALVALLRSPMFAFDEDDLARIALQKDNELDKDCLYDKI
                     QRAVIGRGAHPELIHDTLLGKLNIFLKTLKSWRRYAKLGSLYDLIWKIFNDRFYFDFV
                     ASQAKAEQAQANLYALALRANQFEKSGYKGLYRFIKMIDKVLETQNDLADVEVAAPKQ
                     AVNLMTIHKSKGLQFPYVFILNCDKRFSMTDIHKSFILNRQHGIGIKYLADIKGLLGE
                     TTLNSVKVSMETLPYQLNKQELRLATLSEQMRLLYVAMTRAEKKVYFIGKASKSKSQD
                     ITDPKKLGKLLPLALREQLLTFQDWLLAIADIFSTEDLYFDVRFIEDSDLTQESVGRL
                     QTPQLLNPDDLKDNRQSETIARALDMLEAVSQLNANYEAAIHLPTVRTPSQLKAAYEP
                     LLEPIGVDIIEKSSRSLSDFTLPHFSKKVKVEASHIGSALHQLMQVLPLSKPINQQTL
                     LDALREIDSNEEVKTALDLKKIESFFCDTSLGQFFQTYQKHLYREAPFAILKVDPISQ
                     EEYVLRGIIDAYFLFDDHIVLVDYKTDKYKQPIELKKRYQQQLELYAEALTQTYKLPV
                     TKRYLVLMGGGKPEIVEV"
     gene            40225..41037
                     /locus_tag="VC394_000680"
     CDS             40225..41037
                     /locus_tag="VC394_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEP5994171.1"
                     /translation="MNFKKIRFGFLLCLLLYPLAACNKSEQLNHYERIKKTRKLVVAV
                     SPDYAPFEFKALVNGKDTIVGADVQLAQAIADELDVDLELSPMSFDNVLSSLQTGKAD
                     LAISGISHTKERAKVYDFSIPYYQAENAIVMRASDAKVTKNISDLNGKKVAAQKGSIE
                     EGLVKIQLPKANLISLTAMGEAINELKAGQVYAVTLEAPVAAGFLAQHKDLALAPFSL
                     KTSDGDAKAVALPKNSGDLTKAVNKVIAKLDEQERYKSFIAETIALTKNTMK"
BASE COUNT        12881 a         6969 c         8379 g        12835 t
ORIGIN      
        1 gtgttacaac agtacaattg agctagcctt gtccttgttg tgttaacttt atttttaaaa
       61 taaggttaaa aataaacgac tcgcgttctt atcagttact tattagataa ggaggtaaac
      121 cttatgttaa aatttacttc aaatatttta gctactagtg tagctgaaac aactcaagtt
      181 gctcctggag gctgctgttg ctgctgtact acttgttgct tctcaattgc tactggaagt
      241 ggtaattctc aaggtggtag cggaagttat acgccaggta aataatctat ttagcatctc
      301 tatgtgatag tgatattaag gtaatgagtt tgctagcaac taagttatct ttggtaactt
      361 ctgattatag atgaatggca tagaggtgtt agaaaacatg agacaaaagt aatcttcttg
      421 atgctaacgt ctcaggtaat tagcaggtac tagatagtac ctgctaatta ctatatgttt
      481 agtaaaatga gataggaaag atagttatgt cattttttac aaaggaacaa caacctaaag
      541 agaattgtcc gccaataact gttgaaaaag caaggcaatt gtttgaattt aatacaaacc
      601 acttgtcctt atcggattac catcatcaaa cggtgctaaa aacgtcaaag cagctagttg
      661 ctcaacattt aatgcctaat gcaaccgata atcttagtca acattttttg atgaactata
      721 aagctaataa taattattta ggcttccaag ctagtattgt cgactttttt acagattctg
      781 ccgttgctaa tttttcaagt agttacgttt atgaaagtca ggaaaagata attcgtttac
      841 caaaacctac caagatatca actgctctgt cgacatgtat tataaaacga agaagtcatc
      901 gtcaattttc agatagacaa atgcctcttc aagatttatc aaacattctt tattatgcat
      961 gtggtgttag ttcacaagca tcaattagag atggagcatc agataagatt acactcagaa
     1021 actgtgcttc aggtggaggt ttatacccta ttcatttagt tttttatgct agaaacatca
     1081 gtaaattaat agatggtttc tatgaatatc taccctatca gcatgcacta aggtgttatc
     1141 ggcatagctc tgaggaaaac gttagagatt ttgcggaata cggtgctatt aatgctgaaa
     1201 attgtaatat tattattatt tatgtctacc attacatgaa aaatacacgt aaatatggga
     1261 atcaggcgac tgcctatgct tttattgaat caggagaaat agcccagaat attcaattga
     1321 ctgcaactgc cttaacttat ggaagtattg atattggtgg ttataataag gaatatctcc
     1381 aagaattatt agatttagat gggctaggag agcatgtgat tcacatgaca ctcgtaggaa
     1441 ctaaggagtc tcaatgaaat atcaacttaa tagtaatgtt cgtgtcgtga catttcaaga
     1501 tacattttgt tttagaaaag gaatctggga ttttaatgaa gccattctag atttgacaca
     1561 agaacctcaa aatttaaaga tagtttatca agaaatagtt tcacagttgg ttaagggagt
     1621 agccattgat actgaaactt atgaaaatac tttagaacct gagatgttcg ctaatttaat
     1681 ggaagtcatt tcaggcttgt attacaatga tatgctgatg cttgaggatg actacaatct
     1741 tgaagaaaat gtcatgaaaa ttttaatggg taatttccgt tttatggcgc aagcaggtca
     1801 tatggtaagc aatgctcctg ttttgtttat cagtgattcg acttatgtta atgaatctgc
     1861 tgagttgcta gctgaacaac tgcatttaca attgcaagtg gctagcgatg acttaaaaat
     1921 gttaattcag cagacagatg ttagttcgcg tttagatgct ttggagcatc gccgtaacat
     1981 gaattgttta tcagaagcct tgtgcgatta ccaaagtatt attatttgtc aagaacgctt
     2041 gaatattatg atgttgcgac acttgaatga agttagcgta gctatgaaaa aacaattagt
     2101 gattggtttt gtagatggac cattcttgca cacttgtact ttgaatcctc ctcatagtgc
     2161 tgactttgat agcttagaaa gaagagtttt ggcacgttta caagactcaa ctctctatca
     2221 acattttgct aatcagattt taccagcaac acaagatgtg agccaggcct atttaccatt
     2281 gttaaatgtt ttaatgaatt tagtcgttag cgaagctttc attattgctc aaacaggtag
     2341 ttctaaattt gagggacgtt tattaagcat ttacttaccg acgcttgaga ttcaggtcca
     2401 agatattttg aaaatgtcta attcacagac acaaggagct ttggcaaaat taaaatatga
     2461 ggatcagcag atatcaacac gtgagattgt taaaaaactc cttgacgagt aacctataag
     2521 gagaatctat tatgttatac tattatcctt cttttaatca tatttttgat gaattaaaaa
     2581 gtttaagtgg caatcgaaca ggtatcttaa atcaatctca agttccagtt tgcaaccatc
     2641 ctcacgatgt ttacttaaaa agtattactg gccaaatgcc agattatcat aaacaattta
     2701 taggtgagtt gagtcaagtt agttatcaca ttattggtta cggcagttac tatgaagaag
     2761 cacttatcaa gtatttagga gaaagtattg aacgctatgc tactgtaatt gccggagact
     2821 tactatcaga tcggattgtc tatgcttctt ataatgagtt aaaactgtta cataaagtga
     2881 tgcctttaga atacttgcaa gtgtttactc aagaacagat cgcattatct tgtgacttgc
     2941 aaatgatgat gtgcgataaa atggtaactg agaacgatgt attgggctgg gttaaatgtc
     3001 ccatgttctt tgaggacgca gagatgtatg ttccagctca aatgctttgt gttgggtata
     3061 aaacaaatga gactgtagga gaaagacgga taataccagg attttctacg ggaacagctt
     3121 ctcacaaaac gttagaagcg gctatgtgca acagtttgat tgaatacatc cagatcgatt
     3181 caatgatgct tagctggtat actaagaagc cttgccccaa gattattgtt gatgatcctg
     3241 atattgaagt gattttagaa gaagctagac tgggaaaaga cagtctatac gacattattc
     3301 ctattgatat gactgtagga gaagataatc ctctttatac gtttggaatc attctcaaaa
     3361 ataaatatga tgaagggcct tacctcttgt ttggagtgca ggctgggtta gatcctaaac
     3421 atgctttgct aagagggatc atggaagctt cagctattag ctatagttac tactataatc
     3481 ttttatatca aaaagcttcc cttgcaaata ttgaatgcga agaacctttg tttttagatt
     3541 tagatagtaa tgtcttctat tatgcacatc ccaaggacca agaccataaa tggaaggcct
     3601 ttgaaccttt gatttcagga gaagttctgt taagtgattt ggaagatcat tctggtaaag
     3661 ataaaaaaga agatttgaag actttattag cttatgctaa gaaagtcagt cctaatgctg
     3721 tctttttaga tattacacct cctgaagctc tagaaaaagg ctggtacgtc acgcgagttc
     3781 tgatgccaga acttcttgaa atgtgtatac cagcatttcc ttttgctaat catccacgaa
     3841 tgagacagtt tggaggtgta acaaatgcct ttgtccatcc aatgccttaa tctttgtttt
     3901 ttgttggtaa ctttttgtcc ttcaattcct atgcaagcta tttttggaaa ggaagatagt
     3961 ggttatgcat ttaatttaat tggttttcta agagcaactc tgatttatga cattttggct
     4021 ttggtaagta tttatgtttt gtcacctcaa ataaccttgt ctcttgaaag tattgatagt
     4081 aagacgttct ttatgggact tgtattttgc gtcttgatag tactgattga actcgttttc
     4141 ttacatggtt tacgttgttg gcaaaaaaag caatggcttc ctgcaacttt ctcgtttgta
     4201 ggaacgacaa atgattggtc taagattggc tatcctttat tactagcttt atttgaagag
     4261 acgatttatc gttttttgtg gtttaatata ttagcttttc aatggcattt accaacaatt
     4321 attgttttaa ttgtaactag tttttgctat gctttaaatc acttattgat ggggaaatcc
     4381 attttttatg ctaagttggt aacaggcatc atttatggta gtatttacat gctgactagt
     4441 caattgtggt tggttgtgat aatgcatgta ggagggaact tgctagttga gtgtcttagc
     4501 catcttcaaa caaaaaagaa gaagggggtg acatgatgct attggttttg ctgtcgtttt
     4561 taggtctaac tactctttgg ttgaccagtt atcgaagacg ttgtttggct agatggcatc
     4621 ttcaatggtt agtggcctta tcctatcaag attttttaga tgtcttactg agtctgtttc
     4681 aatttgtcat tattatactt gttttattct tttactcagc tactattaat ctaggagagg
     4741 ttctgacctt tttgacacaa actagctggc attggcagat tctttgttat ttagtgttgt
     4801 atcttatggc catcatagag atgacgcttt tagttttaat tcttatcttt gatgttttgc
     4861 tccagaaaga tagtcgattg ctctttaaaa aaataacttg gctgcctttt agacaagata
     4921 aaccagtctt aagtatactt ttactaggca tagctattct agtagacagt ctcttttatt
     4981 tgggcttgct tatattatta ggagaacagt cgctaacaag tttagcaacg cttattttag
     5041 gttatgggat agtcaaggct tgtcgctatt caggctggtt aaatgtacta ttagcttttt
     5101 gtcttttcac tatagttgga ctatgggcag ttacggcaac tttattatat ggttggctag
     5161 taggtgcgat gatattaacg gtgacttatt tgatgattag ttgcaaagag tattagcaat
     5221 attaaaaagg agtgatatat gagttttgta caattaacaa atgttgtcaa gtcctacaaa
     5281 aacggcaaga aagctgtcaa tgatgtttcc ttgtctattg aagcaggtaa tatttatggt
     5341 ctgttaggac caaatggtgc tggtaagtct accttgatta atcttatctt aggcttgatc
     5401 cctttgagtt ccggtaaaat tactgtttta gggcaatccc aaaagactat tcgaaaaata
     5461 agttcgcaga taggttatgt tcctcaagac attgctgttt atccagacct aactgcttat
     5521 gaaaatgtag aactatttgg gtcactttat ggcttaaagg gagctcagct taaaaaacaa
     5581 gttctaaaaa gtttagaatt tgtggggcta cactcccaag ctaagcagtt tccaagtcaa
     5641 ttctcaggag gaatgaagag acggttaaat atcgcttgtg cgctagttca ttcacctaaa
     5701 ttaatcattt ttgacgaacc gactgtaggg attgatcctc aatcacgtaa tcatatttta
     5761 gagtcgattc gtttgctaaa caaagaaggt gccacagtta tttatacgac ccattacatg
     5821 gaagaagtag aggctctttg tgattatatt tttattatgg atcatggtca agttattgaa
     5881 gaaggaccta aatttgaact ggaaaaacgt tacgttgcaa atctagcaaa ccagattatt
     5941 gtaactctaa cagattcacg tcatttggaa ctggcagata agcctgattg gtctttgata
     6001 gaagatggag aaaaactcat gttgaagatt gataatagtg atatgacatc agttgttcat
     6061 cagctcacac aggccaatat tacttttagc gagattagac ataaccattt gaatttagaa
     6121 gaaattttct tacacttaac aggtaagaag ttacgagatt agaggttact atggttttat
     6181 ttcatttaat caaaaaagaa agtttacaga tttttagaaa ccgaacagcc ttattgatga
     6241 tggtgatttt tccaattttg atgatcgtta ttttaagttt tgcctttaaa tcaagtttta
     6301 atactgcgac aacagtccct aaattgacca ttcgttacca attagagggt gaaaaaacgg
     6361 attaccagaa aaattttctt gcttttttaa aagttttaaa ccaaaaactt catttagagg
     6421 ctaaacctag taattctctt gaaaaagatc gacaaagggt cagtgaagga gccttaacgg
     6481 ctgttttaga agtgaagaag aatcagacca ttaaggttat tactaataat attaatcagc
     6541 aaaatgcaga tttgatcaat atgctagtaa aaaattatgt agataatgct aaaacttatg
     6601 actcgatagc agctctttat cctcaacaat taaatcatat cagaaagcga agtgtggact
     6661 atgttaaggt cagttcaata cagacaagta aaggaatgac atcagctgat tattatgcta
     6721 tttctatgtt taccatgatt actttttata gtatgatgtc tgcgatgaac cttgttttgt
     6781 cagatcgtca acaacggatt acaaatcgta ttcacttaac aggagtttct ccaagtcttt
     6841 tggtctttgg aaaattaata ggtgctatgt tagcaacaac tgttcaattg agtcttttat
     6901 acatttttac aaggtttgtt ttacgagtta attggggcac taatgagtgg atgcttattg
     6961 gtataacagc ctccttagtc tatctctctg tagctatagg tatcgggcta ggcataagca
     7021 ttaaaaatga ggctttttta acggttgcat ccaatactat tattcctata ttcgcctttt
     7081 taggaggcag ttacgttcca ttgacaacat tacacagctc tattattaat caattgtcga
     7141 atatatctcc tattaaatgg gttaatgata gtttgttcta tcttattttt ggtggtcaat
     7201 ataatccgat tcctgtaact ttgatcgtta atattagtat cggaacaatt tttataatat
     7261 tggcattgat aggtatgaga aagcaggtga cgacatgatt tgttttataa aaactctatt
     7321 tgttaagata aagcgcaaaa aaacaagtta tgtgacattc tttttaatgc ctattttaac
     7381 gacacttttg gctttatctt tgagtttttc aaataataat caggctaaaa ttgggatatt
     7441 agataaagat aatagtcaga tatctaaaca atttattgct caactaaagc aaaacaaaaa
     7501 gtacgacatt tttaccaaaa tcaaaaaaga gcatattgat cactaccttc aagataaatc
     7561 tctagaagcg gtattgacta ttgacaaagg attctcagat aaagtgttgc aaggaaaatc
     7621 tcaaaaactc aatatacgtt cgattgctaa tagcgaaatt acagagtggg taaaggcaca
     7681 gaccaattat ctattggaaa actataatat tataggggat gtggctctag gtaacgaaga
     7741 tacttttaat agaattctcc aaaaaaacca acagttaaat tatgatgtta agcaagtaac
     7801 tttgacagac cgatcacgta gcaaagcagt atcttccaca acgacaggat ttttgcttat
     7861 tctaatgtta ggtagtacga gcgtgattta cagtggtatt ttggcagata aatctggtca
     7921 gttataccat cgattaatgt tatcgaactt atctcgtttt cgctatatgt tgagttacgt
     7981 ctgtgtagga tttgttgctt ttacgattca aattgtcatt atgctgagtt tgttaaaagt
     8041 atttaatatt agtttttttg ttccgacatc acttcttttg attatttttt tcctttttag
     8101 tttattagcg ataggctttg gcctgctgat tggagctatc acacaaaatt cccaacaaag
     8161 tagccagtta gctaatctta ttgtaatgcc gacttcaatg ttggctggct gtttatggcc
     8221 attgtctatt acgccttctt atatgcaagc aattggtaag ttattaccac aaaattgggt
     8281 tttatcagcc atagctattt ttcaaagtgg agggacctta tctcaggctt ggccatactt
     8341 gttagctcta atgggaacag cactagcttt gattagtttt tcaagtttat tattaaaacc
     8401 taccaaacta taattagtct tttgttaaaa aatactttcc taattagcta actaccaggt
     8461 agccctttta ttggcttata tgcaataaaa gggttgcttt ttgcttatat tttctctagt
     8521 gcacttatct tttgttaaaa gcgattgcga tgttactgtt gttactgtgt tttttagaga
     8581 gcatattatc aaaagcgacc tctctagaaa gttcgttttt gttgacagat tagttgtcaa
     8641 agtattttaa aaaaacgttt tcaataaaca taaaaactaa tgttattaaa aaaagttgtg
     8701 aaaacgtatt tattttatgg aatgtttttg ttacaataag catgtatcga atgctataaa
     8761 gctgtcttgt tcgctatcag ggatgcttgt gatgttatgt cagatcaaac aaatctttaa
     8821 ttgggtgact taacagatta aaagaaaaga tctaatcgtg ttacttgata gtataattaa
     8881 tgcaaacaga aagtgtatcc caattcatag tctcaggtgg aggttaatat tcaaaaataa
     8941 aatcttgttg ttgtcttaaa ctaatcacat taaaaggaga tatcatgatt aacaagaaat
     9001 atataatacc tgtttcattg ttgacactag ctattacgct tactagtgtt gaagaagtta
     9061 cttcacgcca aaatttgact tatgccaatg aaatcgtaac acaaaggcca aagagagaat
     9121 ctgttattag tgataaatcg aattttcccg tcatatcacc ttacctagca agtgtggatt
     9181 ttggtgagag aaaaacatct ttgccaacac ctgataaagg agtaaaagta actactgaac
     9241 agtctattgc tcaagtaaga aaggggcctg aagaaagaac ctatactgtt actggcaaga
     9301 ttacgagtgt gatcaatggc tggggaggct atggctttta tattcaagat agtgaaggta
     9361 ttggacttta tgtttatcct caaaaagatt taggatacag taagggagat attgttcaat
     9421 taacaggtac acttactcgc tttaaaggtg atttacaact ccaacaggtg actgcacaca
     9481 aaaagttaga gttatctttt ccgacttctg ttaaagaagc agtaatatca gaattagaaa
     9541 caacaacacc ctcaacatta gttaagttat ctcacgtgac agttggagaa ttatcaactg
     9601 atcaatataa caacacatct ttccttgtaa gggatgatag tggtaaaagt atagttgttc
     9661 atatagatca tcgtacaggg gttaaagggg ctgatgttgt tactaaaata agtcagggtg
     9721 atttgattaa cctcacagcc atattgtcta ttgttgatgg tcagttacaa ttaagaccgt
     9781 tttctcttga acaattggaa gtggttaaaa aggtcacaag ctcaaatagt gatgcttcat
     9841 ctcgtaatat tgtgaaaata ggcgagattc aaggagctag tcatacgtcg ccacttctca
     9901 aaaaagcggt caccgtagaa caggttgttg tcacttattt agacgattcc actcattttt
     9961 atgttcaaga tcttaatggt gatggtgatt tagcgacttc agatggtatt cgtgtttttg
    10021 ctaaaaacgc taaggttcaa gtcggcgatg ttttgaccat ttcaggtgaa gtggaagaat
    10081 tctttggtcg tggttatgag gaacgtaagc agactgacct taccatcacc caaattgtgg
    10141 ctaaagcagt gatcaaaaca gggacagctc aagttccatc accgcttgtt ttagggaaag
    10201 atcgtatcgc gccagccaat attattgata atgatggctt gcgtgtgttt gatccagaag
    10261 aagacgctat tgattattgg gaatcaatgg aaggcatgtt agtggcggtt gatgatgcta
    10321 aaatccttgg tccaatgaaa aataaagaaa tttatgtctt acctggctct agtacaagac
    10381 cgttaaataa ttcaggtgga gtattacttc cagctaattc ttataacaca gatgtgattc
    10441 ctgttctttt caaaaaaggc aaacaaatta ttaaagcagg agactcttac aaaggaagat
    10501 tagctgggcc agtatcttat agctatggta attacaaggt ctttgttgat gacagcaaaa
    10561 acatgccatg tttaatggat ggtcatctaa aacctgaaaa aacaaacttg caaaaagacc
    10621 ttagcaagtt aagcattgct tcttacaata ttgaaaactt ctcagccaat ccttcttcaa
    10681 ctaaagatga gaaggtcaaa cggattgccg aatcctttat tcatgatctg aatgctccag
    10741 acattattgg attaattgaa gtccaagata ataatgggcc gactgatgat gggacaacgg
    10801 atgcgacaca aagcgcgcaa cgcctcattg atgctattaa aaaactaggt ggcccaactt
    10861 atcgttatgt tgatattgct ccagaaaata atgttgacgg aggtcaacca ggtggtaata
    10921 ttcgaacagg attcctttat caaccagagc gcgtcagcct ttctgataag ccaaaaggcg
    10981 gtgctcgtga tgctctaact tgggttaatg gagaattaaa ccttagtgtt ggtcgaattg
    11041 atccaactaa cgccgcttgg aaagatgttc gtaaatcgct agcagcagaa tttatcttcc
    11101 aaggtcgtaa agtcgttgtt gttgcaaatc atttgaactc taagcgtggg gataatgctc
    11161 tttatggtcg tgtgcaacca gtcactttta aatctgagca aagacgtcac gtcttggcta
    11221 atatgctagc acaatttgcg aaagaagggg caaaacacca agctaatatt gtgatgctag
    11281 gtgactttaa tgattttgaa ttcacaaaga cgattcaatt aatcgaagaa ggtgatatgg
    11341 ttaacttggt gaaccgacat gatatttcag atcggtattc ttattttcac caaggcaata
    11401 atcagaccct tgataatata ttagtttcac gccatttact tgatcactac gaatttgaca
    11461 tggttcatgt gaattcccca tttatggaag ctcacggacg cgcatcagat catgatccat
    11521 tgttacttca attatcattt tctaaagaaa atgataaggc agagtcttct aaacaaagtg
    11581 taaaagctaa aaaaacttca aaaggaaaac tgttgccaaa aacaggagat agtcttgttt
    11641 atgtgataac gctactagga acggctagtt tattagtgcc tattttatta ttgactaaag
    11701 gcaaaaagga atcatagcga tttgaagaat tggagttcat aaagagtcgt taaaggcttg
    11761 atatacttac gttagacctt aagctaaaat ggatgactat ataaaaacta atattgataa
    11821 caaaagaaga taacaataag acagctttac ttgctaaaga cttttgtcaa gctaattgtt
    11881 ttatggtaca atgtaaccgt tgctttgcaa cccttggtgc ttagttccct ttcaccaagc
    11941 atattagaaa cgtagatagc ttttgagagt tatccagacg ttaaaggaga aaaaatgaca
    12001 aaattgactg tacacgacta tgttcacatt gggctagtag ctgccttgta tgttgtctta
    12061 actattaccc cgcctttaaa tgcgatttct tatgggatgt atcaatttag aatatctgaa
    12121 atgatgaatt tcttagcttt ttatcatcgc aagtatatta ttgcggtgac tcttggatgt
    12181 atgattgcta acttttatag tttcggactg attgatgtct ttgtaggcgg cggttcaact
    12241 cttatttttg tgaccttagg tgttattttg tttagtaagt atcaaaaaga ttatcttttt
    12301 aatggcattt ttaataaagc ttttgtttac ttttctttct tttttgcaac ttccatgttt
    12361 actgtagcaa tcgaacttta cttctttgga gctccatttt tattaacatg gtttacaact
    12421 gcgttgggag aattagtatc tctgctcata ggatcactta ttattgataa gttgtctcaa
    12481 agaatttcct tttagtaatg catcatactc aaaagagttg ggtcaatacc cttctctttt
    12541 ttatgttgtt tgtaatgtta aaatcgttct agatagaaat atttaacact ttagctaata
    12601 gtaccatatg gagtggccaa cgattacttt tgatgctgtt atgatataga aatagctcat
    12661 ttgttatctt ttatgataaa ataaaagtat gaaaaaacgc ataaaagaat taacagatct
    12721 tcttaaccgt taccgttatg actattatac taaagatgcc ccatcagttt ctgacagtga
    12781 ctatgataag ctttatcgtg aactggtgac tttagagcag tcatatcccg agtatgtctt
    12841 gcaagatagc ccaacgcagc aggttggagg gactatttta aaggggtttg aaaaatatcg
    12901 acatcagtat ccgcttttta gtttgcagga tgctttttca cgtgaggagc tggatgcttt
    12961 tgataagcgc gtcaaggccg aatttccgaa tgccacctat ctagcagagt taaaaattga
    13021 tggtttatct atttccttga gttatgagaa tggcttctta caggttggag caacacgggg
    13081 tgatggcaat atcggtgaaa atatcacaga aaatattaaa aaaataaagg atattcctca
    13141 ccaattgagt gagcctttaa ccatcaccgt tcgaggagag gcttacatgt ctcgtcagtc
    13201 ctttaaagcg atcaatgaag cacgccaaga aaatggggag actgaatttg ctaatcctag
    13261 aaatgctgcc gctgggacac taaggcagct agatacggca gtggttgcta agcgtcaact
    13321 agccactttt ttgtatcaag aagctagtcc gacagctaga aatcaacaaa atgaggtctt
    13381 agcagaatta gcagatctag gtttttcagt aaatccgtat tatcaactca catcgtctat
    13441 ggatgagatt tggaatttta tcaaaactat tgaggcaaaa agagatcaat tagcttatga
    13501 catagatggc gttgttatta aagttaatag cctagccatg caagaagagc tagggtttac
    13561 ggttaaagct ccacgctggg ctattgctta caaatttcca gcagaagaaa aagaggcaga
    13621 aatcctttcg gttgactgga cagtgggacg aacaggcgtg gtcacaccta cagcgaatct
    13681 aactcctgtg caactagcgg ggacaaccgt gagtcgtgca accctacata atgtggatta
    13741 tattgccgaa aaagatatcc gtattggcga tacagtgatt gtatataagg caggggacat
    13801 tattcctgct gtcttaaatg tcgttatgag taagcggaac cagcaagaag tcatgcttat
    13861 tcctaaacta tgcccatctt gtggtagtga attagttcat tttgaagatg aggttgcttt
    13921 gcgttgtatt aatccgttgt gtcctagttt gattcagcga agtttggagc attttgcaag
    13981 tcgtgatgcc atgaacatta ctgggctggg accagctatt gtggaaaaac tatttttagc
    14041 ggggtttgtt catgatgttg ctgatattta ccagttgacc aaagaagatt tcatgcaatt
    14101 agatggcatc aaagaaaagt cagctgataa attgctcgct gcgattgagg cttctaaatc
    14161 aaattcagct gaaaaactgt tatttggtct aggaattcgt catattggct ctaaggttag
    14221 tcgcttaatt ttagaggtat acggagatat ttctgctctt ttaaccgcta aagaagagga
    14281 aatcgcaagg atagatggtc ttggctcaac gattgctcaa tcacttactc aatactttga
    14341 acaaaaaacg gctgcgattt tagtagacga gttaaagaca gcaggtgtca acatgcatta
    14401 ctcaggtcaa aaagttaata gtgacgcagc gctttttggt ctaactgttg ttttaactgg
    14461 caaattaaat cagctgaacc gaaatgaggc caaagataaa ttagaggcat taggtgccaa
    14521 agttactgga agtgtatcta agaaaacaga tttagttatt gcaggtagtg acgctggttc
    14581 taagctagaa aaagcaaaga gcttaggaat tcggattgaa gatgaagatt ggctacgcca
    14641 gctttaagtg aggtaatgtt atgaaaaaac aattgagagc acgtctcatt tataatccaa
    14701 cgtcaggaca agaacttatg agaaaaagtg ttcctgaagt tttagacata ttagaagggt
    14761 tcgggtatga aacatctgcc tttcaaacaa cagcccaaaa gaactctgct ctaaatgaag
    14821 caagaagagc ggcaaaggca ggttttgact tattgattgc tgctggaggt gatggcacta
    14881 ttaacgaagt ggtcaatgga attgctccct tgaaaaagcg ccctaaaatg gccattattc
    14941 caacaggtac cactaatgat tttgctagag cgttaaaagt accaagagga aatccgagcc
    15001 aagcagcaaa acttatcgga aaaaatcaga ctatccagat ggatattggt agagctaaga
    15061 aagatactta tttcatcaat attgctgcag caggaagttt gacggaatta acttacagtg
    15121 taccaagtca gttaaaaacg atgtttggat acttggctta tcttgctaaa ggagtagaac
    15181 tgctcccaag agttagcaat gtgcctgtta aaattacgca tgacaaaggc gtctttgaag
    15241 gtcaagtttc tatgattttt gcagctatta ccaattctgt tgggggattt gaaatgattg
    15301 ctcctgatgc aaaactagat gacggtatgt ttacgctcat tttaataaag acagcaaatc
    15361 tttttgaaat tgtacatctt ttgcgtttga ttttggatgg tggtaagcat attacagatc
    15421 gtcgtgtcga atacattaag accagtaaaa tagtcattga accccaatgt ggtaagcgaa
    15481 tgatgattaa tttagatggc gagtacggag gagatgctcc aattacatta gagaacctaa
    15541 aaaatcatat cacttttttt gcagatactg atttgatttc tgatgacgct ttagtcttag
    15601 atcaggacga attggaaatt gaggaaatcg taaagaaatt tgctcatgaa gtagaagact
    15661 tagaacaaga attagaagaa taatacaata gttattagct actgacagtt tagtcaaatg
    15721 cactactatt agaatctaag atttttaaat taattgaggt agtgtttttt gatgtgctca
    15781 aagagtcaat ttattcatat gaggggccca tttgaggtct ctcttttttg atcatatgca
    15841 agaaaatgcc atgttttagg gatgttatta tacccttttt ggaatcttgt tgcctttttg
    15901 ttataataag agtgtaattc tatttttaaa aagatcttaa caaagacaga ctcgtttatc
    15961 aaaaaggtta gtattagtct aagaaagcgt actcagtcta aagaaatctg ttgacattct
    16021 taattttaag gaatagaata gcaaagtaaa aaaaatttaa aggagtattc tactatgaat
    16081 cctattttcg cattggcttt ggcctgtttt ggtgtttctc ttgccgaagg tttcttgatg
    16141 gcaaatcttt ttaaagcagc atctcgccaa ccagaaatca ttggtcaatt acgttcatta
    16201 atgatcttgg gtgttgcctt tatcgaaggt actttctttg ttacccttgt tatggccttc
    16261 attcttaaat aacagcctat ttgataatag aaaaggaggg tgttagatgg aagaagctaa
    16321 aatacccatg ctaaagcttg gtccaataac ctttaatttg accctacttg ctgtttgtat
    16381 tgtcacaatt gctgttatct ttgcctttgt tttttgggca agtcgccaaa tgaaattgaa
    16441 accagaaggg aagcaaactg ctttagaata tttaatcagt tttgtggatg gtattggaga
    16501 agagcactta gatcataatc tacaaaaatc ttattcgctg ttactcttta ccatttttct
    16561 ctttgtggct gtcgctaata atttagggtt atttactaag ttagagacag ttaatggcta
    16621 taacctatgg acctcgccaa cagccaatct ggcttttgac cttgctctat ctctttttat
    16681 taccttaatg gtacacattg aaggggttag acgtcgtggc ttggttgccc atttgaaacg
    16741 tttggctaca ccgtggccaa tgactccgat gaatttatta gaagagttca caaatttctt
    16801 atcacttgcc attaggttat tcggtaatat ctttgccggg gaagttgtta caggtttgat
    16861 tgttcaactg gccaattatc gagtttattg gtggccgatc gctttcctag tcaatatggc
    16921 gtggacagcc ttttcagtct ttatttcctg catacaggct tttgttttca caaaactgac
    16981 agcgacctat ctaggaaaga aagttaatga atcagaagaa taaggaagga gaacaaacga
    17041 atgtcaatca catttggtga actcgttggg aattttattc tagtaacagg ttctgtaatc
    17101 gtcttgttac tcttgatcaa aaaattcgct tggggtgcaa ttgaatcgat tttacagaca
    17161 cgctcacaac aaatctctcg agatattgat caggctgagc aatcacgttt aagtgctcaa
    17221 cagttagagg caaaaagtca agctaaccta gatgctagtc gttcacaagc aagtaaaatc
    17281 attagtgatg ccaaagaaat tggtcaatta caaggtgata aattggtggc agaagctact
    17341 gatgaagcaa aacgcttgaa agaaaaagcg ttgacagata ttgaacaaag caaatcagac
    17401 gctatttcag cagtcaaaac agaaatgtct gatttaacgg ttcttttagc ggaaaaaatt
    17461 atgggagcca atcttgataa gacggcgcaa agccagctta ttgacagtta tcttgatgac
    17521 ttaggagaag cttaatgacc aaaaaagaac aggctcttat cgagcaatat gcaaaaagcc
    17581 ttgttgaggt agcaagtgaa catcattctc ttgatgcctt acaagctgat gtgcttgcga
    17641 ttttagagac gtttgtaaca actaatcttg atcaaagtct atcaagtcta gctgttccgc
    17701 atgctgaaaa aataaaattg ctaaccttat taaagggaaa taattctgtt tatatgaata
    17761 atttcttgaa tctcatttta caaaacgaac gtgaagctta tttgtatcaa atgcttcaga
    17821 ctgttttaaa tgaaattgca atagtttcaa atcagtatga tgtgactgtt acctcatcac
    17881 tacctttgac tgaggagcaa aaaagccgtg ttcgagcagt tgttgcaaag aaatttgctg
    17941 taacagcggg tcgtttaatt gaaaaagtag atccttcgct tattggagga tttattatca
    18001 gcgtcaataa taaagtaatt gataccagta ttcgtcgtca attgcaagca tttaaaatga
    18061 atttaaaata gaaagtggtg tgacttttgg caattaatgc acaagaaatt agcgctttaa
    18121 ttaaaaagca aattgagaac ttccagccaa atttcgatgt cacagaaact gggatcgtta
    18181 cctatattgg tgacggtatt gcacgtgccc gtggattaga taatgccatg agtggtgaac
    18241 ttcttgaatt tgaaaatggt gcctatggga tggctcaaaa ccttgagtca aatgatgtag
    18301 gtattattat tcttggtgat ttttcagcaa tccgtgaagg agatgtggtt aagcgcaccg
    18361 gtaaaatcat ggaagtacca gttggtgaag ctctcattgg acgtgtggta aacccacttg
    18421 gacaaccagt tgatggcttg ggagatattg aaacaactgg ctttagacca gtcgaaacgc
    18481 cagctcctgg tgttatgcag cgtaaatccg tttctgaacc cttacaaact ggattaaaag
    18541 caattgatgc tttggttcca attggtcgtg ggcaacgtga gttgattatc ggagaccgtc
    18601 aaacaggaaa aacatccgta gctattgatg cgattttgaa ccaaaaaggt caagacatga
    18661 tttgtatcta tgtggctatt ggtcaaaaag aatcaaccgt tcgtacacaa gttgaaaccc
    18721 ttcgccgtta cggtgccttg gattatacta ttgttgtgac agcgtcagcc tcacaaccat
    18781 caccattgct cttcatcgct ccttatgcgg gtgttgccat ggctgaagaa tttatgtacc
    18841 aaggcaagca tgtcttgatt gtctatgatg atttatcaaa acaagcggtg gcttatcgtg
    18901 aattgtctct cttgcttcgt cgtccaccag gtcgtgaagc ttacccaggg gatgttttct
    18961 acttgcatag ccgtcttttg gaacgctctg caaaagtttc tgatgactta ggtggaggtt
    19021 ctatcacagc tctaccattt atcgaaacac aggccggaga tatctctgca tatattgcga
    19081 ctaacgttat ttcgattaca gacggacaaa tcttcttgca agagaacctc tttaattcag
    19141 gaattcgtcc agcgattgat gcaggatcat ctgtctcacg tgtaggtggt tcagctcaaa
    19201 ttaaagcgat gaaaaaggtt gctggtactc ttcgtttgga tttggcttcc tatcgtgaat
    19261 tagaagcctt tacccaattt ggttcggacc ttgatgctgc aacacaagca aaacttaatc
    19321 gtggtcgccg tacggttgaa atcttgaagc aaccattgca taaaccgtta cctgttgaaa
    19381 agcaagtcgt tattctttat gcccttactc atggtttctt agatgatgtt cctgtagatg
    19441 atattttggc ttttgaagaa gctctttacg actactttga cgttcattac aatgatttat
    19501 ttgaaactat ccgtaccaca aaagaccttc ctgaagaagc tgcccttgat gccgctatta
    19561 aagcgtttaa agaacactca aactttaagt catagaggga ggatactagt atggcaggct
    19621 ctctaagtga aattaaagct aaaattattt caactgagaa aacaagtaaa atcactagtg
    19681 ccatgcgtat ggtttcttct gcaaaacttg ttaaatctga gcaagcagct cgtgattttc
    19741 aaatttacgc ctcaaaaatt cgccagatta ctacagattt attgaaatca gaattaacca
    19801 taggatctga taatccgatg ttggtgtctc gtcctgtcaa aaaaacaggc tatattgtca
    19861 tcacgtctga taaaggactt gttggaggtt acaattcaaa aatcttgaaa tctgttatgg
    19921 atatgattac agagtaccat gctgatggtg actatgaaat catttctatt ggtagtgttg
    19981 gttcagactt cttcaaagca agagggatga atgtggcttt tgaacttcgt ggacttgcgg
    20041 atcaaccaag ttttgaacaa gtgagacaaa tcatttcaca atccgttgac atgtttgtca
    20101 atgagatttt tgatgagtta tatgtctgtt acaatcacca tgtgaatagc ttaaccagcc
    20161 aagtccgtgt tcaacagatg ttgccaattt ctgacttggt tgctgatgaa gcagcagaag
    20221 aaggtgttac tgggtttgaa ttggaaccta atcgtcatga tattttagat caattgttac
    20281 ctcaattcac agaaagttta atttatgggg ctatcattga tgctaaaacg gctgaacatg
    20341 cagcaggtat gactgccatg cagacagcga cagataacgc taagaatgtc attaacgatt
    20401 tgaccatcca atataaccga gctcgtcaag cagcaattac acaagaaatt actgaaattg
    20461 ttgcaggagc aaatgcacta gaataaaaaa taacgctgac tagcgattag gttattgtca
    20521 gcattcaatt ttatcaatcg agatgaaatg acttatggga cttttctatt gaaagactgc
    20581 tcttttcagt ataaaagaag ttagcaagac taactaagcc ctgactatcg aatataagga
    20641 gaaatgaatg agctcaggca aaattgctca ggttgttggt ccagtagtag acgttatgtt
    20701 tgcgagtggg gacaaacttc cagagattaa taatgcattg attgtttata aagatagtga
    20761 taaaaagcaa aaaatcgtcc ttgaagttgc tctggaactt ggtgacggta tggtgcgaac
    20821 aatcgctatg gaatcaactg atgggcttac acgtgggtta gaagttcttg acactggtcg
    20881 tgcgattagc gtaccagtag gtaaagaaac tttgggacgc gtctttaatg tacttggaga
    20941 aaccattgat ttggaagaac catttgcaga agatgttgac cgtcagccga tccataaaaa
    21001 agcaccatcg tttgatgaat tatcaacatc atcagaaatt cttgaaactg gtatcaaggt
    21061 aattgacctt cttgcccctt accttaaagg tggtaaagtt ggacttttcg gtggtgccgg
    21121 agttggtaag actgtcctta tccaagaatt aatccacaat atcgcccaag aacacggagg
    21181 tatttcagta tttaccggtg ttggtgagcg aacacgtgaa ggaaatgacc tttactggga
    21241 aatgaaagaa tcaggcgtta ttgaaaaaac cgccatggtt tttggtcaga tgaatgagcc
    21301 gcctggggca cgtatgcgtg tagcccttac tggtttaacc attgctgagt atttccgtga
    21361 tgtagaaggc caagatgttt tgctctttat tgataatatc ttccgtttca ctcaggcagg
    21421 ttcagaagta tcagccctct taggccgtat gccttctgct gttggttacc aaccaacact
    21481 tgctactgaa atggggcaat tgcaagaacg gattacatca actcaaaaag gttctgttac
    21541 atctatccaa gcgatttatg taccagcaga tgattatact gaccctgcgc cagcaacagc
    21601 atttgctcac ctggattcaa caactaacct tgaacgtaaa ttgacacaaa tggggattta
    21661 tccagccgtt gatccattgg cttcaagttc acgggcttta tcacctgaga ttgttggtga
    21721 agagcactat gctgtggcaa cagaagttca acgtgtgctt caacgctacc gtgaattaca
    21781 agacatcatc gctattttgg ggatggatga attatcagat gaagaaaaaa cattggttgg
    21841 acgtgcgcgt cgtatccagt tcttcctctc acaaaatttt aacgttgctg aacaatttac
    21901 cggtcttcct ggttcttacg tgccagttgc tgagacagtt cgcggcttta aagaaattct
    21961 tgaaggaaaa tacgatgatc ttccagaaga cgcctttcgt tcagttggtc caatcgagga
    22021 tgtgattaaa aaagccgaaa aaatgggctt ttagtgaggt gatgccatga cacaaatgac
    22081 tgttcaagtg gtaacgccag atggaatcaa atatgatcac catgctaaat gtatttcggt
    22141 aacaacacca gatggggaaa tggggatttt acctaatcac atcaatttaa ttgcaccttt
    22201 gcaagtccat gaaatgaaaa ttcgccgagg tggtgaagat gaaaaagtgg actggattgc
    22261 tatcaatggt ggtattattg aaatcaaaga taatgtggtt accgttgtcg ctgattctgc
    22321 agaacgtgac cgtgatatcg atgtcagccg agcagagcga gcaaaactaa gagcagaacg
    22381 tgaaattgca caggctgaaa cgactcacaa cattgatgaa gttcgtcgtg ccaaggttgc
    22441 cttgcgccgc gctttgaatc gtatcaacgt tagtaaaaag taagtggtgt tgataaaaga
    22501 caaggataag taagaaaggt ttttaaccta ttattttgtt tgctgagtct ttttaacatc
    22561 agtttctgag agtttgacca tactaccttc tctaaacacg ttattattgt tgagaggagt
    22621 tatatggggg aactcttttt tgtatccacg ttgttagtag aaactcagta atgacgctta
    22681 atgtattttt aggctatcaa cttagtcaga gaagatgtca acttaattga taagttggac
    22741 ggatcaagat ggtatgttat aataatccta tggaattatt aatgaagatc attgcctttt
    22801 cgtgtcattt attttttatt ggccttagtt accaattact gattagtgtg attgactgga
    22861 caaaatttag tcattatcat ccagaaaatc ttggccggct acgacttttt gtctttttat
    22921 tagctactgc cctagggtat ttggtgagcc attgtatgct ggaattgctt caaattagcc
    22981 agacgctctt ttttgagttc cgctaatact ggaataaaag aagtatggta taattttact
    23041 acgactaaga gatagataag ggagaaaatt gtggataaaa taatcattga aggtggtcaa
    23101 acacgcttag aaggagaagt tgttattgag ggtgctaaaa acgcagttct tcctttactg
    23161 gcagcatcta ttttaccttc aaaaggaaaa acaattctta gaaatgttcc tattctatct
    23221 gatgttttta ccatgaataa cgtggtacgt ggtttagata ttcgtgttga ttttaatgaa
    23281 gcagctaacg aaatcacagt tgatgcctca ggacacattt tagatgaagc cccttacgag
    23341 tacgtgagcc aaatgcgtgc ctctattgtt gttttaggac caattttggc acgtaatggc
    23401 cacgcaaaag tatcaatgcc tggaggctgt acgattggca gtaggcctat taatttgcac
    23461 ttaaaaggat tagaggctat gggggcaacg attactcaaa aaggtggaga tattaccgct
    23521 caagcagatc gactgcaagg tgccatgatt tatatggact tcccatctgt aggagctacc
    23581 caaaacttga tgatggctgc cactttagct gatggtgtga ccactattga aaatgctgct
    23641 cgtgagcctg aaattgttga tttagctcaa tttttgaata aaatgggagc tagaattcgt
    23701 ggtgctggaa cagagacgtt aacgataaca ggcgtcacaa gtcttcatgg ggtggagcac
    23761 gatgtggttc aagaccgtat tgaagcaggt acttttatgg ttgcagcggc gatgacttca
    23821 ggcaatgttt tgattagaga tgctgtttgg gaacataatc gtccattgat ttcaaaatta
    23881 atggaaatgg gtgtttctgt aacagaagaa gaatatggta ttcgcgttca agcaaacacg
    23941 ccaaagctca aacctgtaac ggttaaaacc ttgcctcatc caggttttcc aacagacatg
    24001 caagctcaat ttacagcttt aatggcagtg gtcaatggag agtctactat ggttgaaact
    24061 gtctttgaaa atcgatttca acatctggaa gaaatgcgac gcatgggctt acaatctgaa
    24121 atccttaggg aaactgccat gattcatggt ggaagacaac tgcaaggtgc tcctgtgatg
    24181 tcaacagatt tacgtgccag tgctgccctt atcttgacag ggattgttgc tcaaggagtg
    24241 acaatagtta ataatttggt tcatttagat cgtggctact atcaatttca tgaaaaatta
    24301 gcaaagcttg gagctactat tagtcgtagc agtgaggtct agcttatgac aaatggttta
    24361 aaatatgtct tagaacaaat gttattactt ttagtaattg ctgccttggc gtgtcttttt
    24421 ttagccattg gacttatgat tggttatagt tttatgggag atggccatag tccatggcat
    24481 attttatcta tggataagtg ggcagagtta gtgaataaat ttactggaaa gtaggccttg
    24541 cctacttttt atagcatttg agaggaagta tgttaacaag gaaaaaacga aaaaggatgc
    24601 ttaattcctt cttattgatt ttgttagtgt tggggtctaa cagccttgct aagagtgatt
    24661 tcatagctag cactcaccct atcaagcaac tttatcagac tcttgttggg gagaagaaaa
    24721 aatcattact cacacataat aatttgcccg ctgatgttcc cacagaagct ctggcggcat
    24781 cggtattgac accatcaatc aaaaagcaac taggtccaga tgttcagtgg aatggagcag
    24841 gcgcctatat tatcaacagg aacaaaacag atttaactgc taaggtagcc agtccgcctt
    24901 atgccaacaa tgaaaccaaa tgggtgcaag agcaaaaggt tcctactaga gctaatgcct
    24961 tacttactaa gtcgacacga cagtatcaaa atcgctatca aacgaacaat gcttatcgta
    25021 cctttaagcc agcaggttgg caccgattgc accacttaaa aggaagttat gatcacgcgg
    25081 ttgatagagg gcatttgcta ggttatgctt tagttggtgg actgaaaggt tttgatgctt
    25141 accagtaatc ctgataatat tgccactcaa ctaagttggg ctaatcaagt aaataaaccc
    25201 tactcgacag gtcagaacta ctatgaaggt ttagtacgtc gtgctttgga taaggggcat
    25261 cgcgtccgct accgtgtcac cctactttat gatggtgata atctactagc cagcggtagc
    25321 catttggagg ctaaatcctc tgatgacagc ttgactttta acgtgtttgt tccaaatgtc
    25381 caaccaggat tgaccgttga ctatcggacg ggacaaatag ctatcaatct ctaagcgaaa
    25441 agtcactgat ttaagattgt cactaagcac tttttttgat agaatagaac taattgaata
    25501 agtgtctatg agactagtac taatagagaa attaacaggg agtgagggcc gtgactggaa
    25561 gccttcttaa gggaactatt aggaattcac tcagctaaag acaattaatt aaggtctgat
    25621 aacatcagat aaaaacggat ggtaccgcgt gtcaacgctc cgcttatgaa gtggagtagt
    25681 ggcacgcttt tttcatcgct aaaagaaggg aaaactatgg atttacaagc acaattagag
    25741 gaattaaaaa caaaaacatt ggagactctc caatctttga cggggaatca taccaaagaa
    25801 ctgcaagatt tgcgcgtggc tgttcttggg aaaaagggat ctttgacaga attactaaag
    25861 gggctaaaag acttgtctaa tgacttgcgt cctgtggttg gtaaacaggt gaacgaagtt
    25921 cgtgacttat tgacaaaagc ctttgaagaa caggctaaaa ttgtagaagc tgctaaaatt
    25981 caagcccagc ttgatgctga aagcattgat gtgactttac cgggtcgtca aatgactctg
    26041 ggccaccgtc atgtgttaac acaaaccagt gaagaaatcg aagatatttt cttaggaatg
    26101 ggttttcaga ttgttgatgg ctttgaggta gaaaaagatt attacaactt tgagcgcatg
    26161 aatttgccaa aggatcaccc tgcccgtgac atgcaagata cgttttatat cacagaagaa
    26221 atcttgttac gaactcacac cagtcctgtg caggcccgta ctcttgatca acatgatttt
    26281 tcaaaagggc ctttaaaaat ggtttctcca ggtcgtgttt tccgtcgtga tactgatgac
    26341 gctacccact cacatcaatt ccatcaaatt gaaggtttgg ttgtgggcaa aaatatttcc
    26401 atgggagacc tcaaagggac tcttgaaatg attatcaaga agatgttcgg tgaagaacgt
    26461 agcattcgtt tgcgcccgtc atatttcccg ttcacagaac catctgttga agtggatgtt
    26521 tcttgcttta aatgtggtgg gaaaggctgt aatgtctgca agaaaacagg atggattgaa
    26581 attcttggtg ctggtatggt tcacccaagt gtcttggaaa tgtcaggtgt tgatgcaaaa
    26641 gagtattcag gctttgcctt tggtttaggt caagagcgga ttgccatgtt gcgttatggc
    26701 attaatgaca ttcgtggttt ctatcaaggc gaccaacgtt tctctgaaca atttaactaa
    26761 aatgagtcgt ttgaagactt ctgtcagcta ttttatctaa gattgagtga aagttgatag
    26821 gtaatgataa taggttaatt gtctaagggg aaagtccctc tcgcttcaac gtcataaggg
    26881 ttttgacttg gaacttaaaa tacgaaagga aatgactcaa atcacggctt gagagctttc
    26941 tctagccgag tgatttgggc actaaaatta tgttagtatc ttataaatgg ttaaaagaat
    27001 tagtagatat cgatgtgaca ccagcagcac ttgctgagaa aatgtcggca acgagtatcg
    27061 aggtagaagg aattgaagtc cctgcagatg gtttatcaaa actggttgtg gggcatgtgc
    27121 tttcttgcga agatgtgcca gagacacatt tgcatttgtg tcaggtagat acaggtgatg
    27181 aaacgccacg tcaaattgtg tgtggcgcac caaatgtgaa agcagggatt aaggttatcg
    27241 tggctgtgcc aggtgctcgt attgcggata actataaaat caaaaaaggc aaaatccgcg
    27301 gcatggaatc tcttggcatg atttgttcct tgcaagaact tggtttgtca gatagtatta
    27361 ttccaaaaga attttcagat ggtattcaaa tcttgccaga ggaggctgtg ccaggtgatg
    27421 ctatttttaa atacctggac ttggacgatc atatcattga attgtccatt acacctaacc
    27481 gtgctgatgc gctttctatg cgaggtgtgg cacatgaagt cgcagctatt tatggcaagt
    27541 cagtatcatt cccccaaaaa aatctacaag aatctgataa ggcaacttca gaagccattg
    27601 aagtggccat tgcctctgat aacgttctaa cttacgctag ccgtgtcgtt gaaaatgtca
    27661 aagttaagcc aagtccacaa tggttgcaaa accttttaat gaatgctggt attcgtccta
    27721 ttaataatgt ggttgatgtg accaactatg tgctcttgta ttttggtcaa cctatgcatg
    27781 cttttgatta tgataaattt gaagaccata agattgtggc gcgtgcggct cgtcaagggg
    27841 aaagtttagt cacacttgat ggtgaaaaac gggatttgac cactgaggat cttgttatta
    27901 ccgtggctga caaaccagtt gctttggcag gtgttatggg tggtcaagcc actgaaattg
    27961 atgccaactc tcagacggtt gttcttgaag ttgctgtttt tgatggcaaa tcgattcgta
    28021 aaaccagtgg tcgtttaaac cttcgttcgg aatcctcttc acgttttgaa aaaggggtta
    28081 actatgcaac tgttttagaa gcgcttgact ttgcagcggc tatgttacaa gaattagcag
    28141 aaggtcaagt cttatcaggc catgttcaag ctggacaatt gccaacagaa cctgttgaag
    28201 tctcaactag ccttgactat gtgaatgttc gtttaggaac agaattaact tttgctgata
    28261 ttcaaaggat ttttgaccaa cttggatttg gtctaacagg agatgaaaca agcttcacgg
    28321 tagctgttcc tcggcgtcgt tgggatgtta gtattccggc tgatttggtg gaagaaattg
    28381 cgcgtattta tggttatgat aagttgccaa caaccttacc agaggcagga ggaacggctg
    28441 cagaattaac gccaacacag gctcttcgtc gtaaggtccg tggtttagcg gaaggattgg
    28501 gcttgactga aatcatttcg tatgccttga caacaccaga gaaagctgtt gagtttgcag
    28561 tggcaccaag tcacctaaca gaactcatgt ggccaatgtc tgtggaacgt tcagcgcttc
    28621 gtcaaaatat ggtttcaggg atgttagata cggtagctta taatgtggct cgtaaacaaa
    28681 gtaacttggc tctttatgaa attggaaaaa tctttgaaca agaagctaat ccaaaagaag
    28741 accttcctaa tgaattgaac cattttgctt ttgctatttg tggcttagtg gcacaaaaag
    28801 atttccaaac ccaagctcag gcagtggatt tttaccatgc taaaggtatc ttggatactt
    28861 tatttgcaaa ccttaacctc aaggttcaat atgtgccaac caaagaccta gcaagcatgc
    28921 acccaggacg gacagcgctt atcctattag atgagcaagt gattggattt gttggtcaag
    28981 ttcatccagg aacagcaaag gcttatagta ttccagaaac ctatgtggca gaattggata
    29041 tggctgcttt agaagcagcg ttgccaagtg atcaaacttt tgcggaaatc acaaaattcc
    29101 ctgctatgac acgtgatgtt gccttgttac ttgaccgtga agtgagtcat caggccattg
    29161 taacagctat tgaatcagca ggtgtaaaac gcttgactaa gattaagctc tttgacgtgt
    29221 atgaaggagt aactattcag gctggcaaaa aatcaatggc ttatagcctt actttccaaa
    29281 atccaaatga caatttaacg gatgaagaag ttgccaaata catggagaaa atcaccaaag
    29341 cattgacaga acaagtcggt gctgaagtac gctaagaaaa aaactgggcc tctaaggctc
    29401 cgtttttttc tgttataata gaggaaaaaa gaactccagt tcaaatgatt ggagaaaaac
    29461 atcagaaggt ggaggaaaat cagaatgtat caaacaaaaa taacaggcaa ccgcctttac
    29521 cacacggttt cgaaaggcta cggagatagt gtaaccttat ttggaaaaac tgatcaaggg
    29581 gaaaccccta tgagtttact ggttattgcc ttgtcttctt gtgtgactat gtgtgtgcag
    29641 ggtttttacc atcgtcaatt tcagcaagac acacttgctg tttctgttga ggctagttat
    29701 gaggaagaag tttttgaact acttgttagg attgatgatg ttttagatga caaaaagaaa
    29761 gctgatttgt tggcttacat tggaacttat tgtcgtgtga aacaactcct caaacctgaa
    29821 gttatggtga ccattacgtt ggaaggactg tctcatgtct gatgctggtt tgaaaactta
    29881 ccaaaaacaa tgggcttatc aaaagtattg ggtcatggca cactcacagc agcattacaa
    29941 tgctttaaga gaattgttta aaggaaatca gtggtcagaa gaaaaggtgt tgacttttca
    30001 ttgtttgatt gaagaagcgc aagccattcc tcccacagta aaaagcttac gaacagcata
    30061 tcagcatgtt tgggggtatt ttaaaaaggt agcgagtcag gaagaaaaag accatttcaa
    30121 agatttggat gctcagttgg aaactaaatc tgaggagatg ctatgctttt tacaagagat
    30181 gacggctcat tatcagccct cttatctgct atcttgccgt ttgattacca aaggtcctta
    30241 aatgctaaac agaaaagtag ggacttctgg gaagttatgc taagctagtg atataaacaa
    30301 gggggaatga gagatgtttt tagcgcttaa tgaaatgaaa caatccaaat tgcggtatgg
    30361 tttgattgct ggtttgctat gtttggttgc ctatttgatg ttctttttgt caggattggc
    30421 ctttggtttg atgcaggaga accgctcggc ggttgatctt tggaaagctg acagtatttt
    30481 actagccaag gatgctgacg ctaccttgac cttatcacag gtgtccagag ctcaagaaaa
    30541 tcaaataaca gcagacaagg tagctcctct tgctcaactc aataccgtgg cgtggtcagt
    30601 taaaaatcct aaggatgccg acaaagttaa ggttagcctt ttcgggattg attctaatag
    30661 ctttattcgt cctaacattg taaaaggtcg attatttaag actaacaaag aggttgtttt
    30721 ggatcaaagc cttgcaaaag aggaagcttt tgcgattggc aaggactttt acacatcgag
    30781 ttcgagtcaa gcattaacta tcgttggtta tactcaaaat gctagattta gtgttgcacc
    30841 agtggtttat atgaatctgg aagcttttga aacattaaaa tatggagaac cactaccaaa
    30901 agataagcaa gttgttaatg cttttatcac taaaggaagt ttaacagatt atcctaaaaa
    30961 agacttccaa aaattagata ttaaaacctt tattactaaa ttacctggtt atagcgctca
    31021 acttttaacg tttggcttta tgattagttt tcttgtcatt atttcagcta ttattattgg
    31081 tatttttatg tatattttga ctattcaaaa ggcacctatt tttgggatta tgaaagcgca
    31141 aggaatttct aacaaaacga ttacgaccgc tgtgcttatg cagacattct ttttgagttt
    31201 tttaggtagt ggtttagggt tgctaggtac ttggctgaca tcattattat taccaacagt
    31261 agttcctttt caaagcaatt ggtttttata tttggctata ttcgttagta tgatctgttt
    31321 tgctctatta ggcaccttat tttctgtttt taacattata cgaattgatc ctttgaaagc
    31381 aattggatag gaggtagtga tgtcggtttt aacatttaaa caagtgacca aaacatttca
    31441 agatggtcat cacgaaatca acgcattaaa ggcaactgac ttttccattg aagctggaga
    31501 atttgtggct attattggac catcaggttc tggaaaatca acctttttga ccattgctgg
    31561 tggcttacag accccaagtt ctggtcagct aatcattgat ggcactgatt atacgcattt
    31621 atcagaaaaa gagcgctcac gcttgcgctt caagtccgtt ggttttatct tacaagcttc
    31681 caatctgata ccttttttaa cagtccagca gcagttggaa ttggtcgatc atttgacagg
    31741 aagcaaagaa aaagccaaag ccaatcagtt atttgacgat ttaggcatta cagggctaaa
    31801 acatcagtta cctcaagaat tgtccggcgg agaaaggcaa agagcggcga ttgcccgtgc
    31861 tctctaccat gatccagcac tgatattggc agacgaacct actgcaagtt tggatactga
    31921 aaaagcctat gaagtggtca aactgttagc taaggaaagt aaagaaaaaa acaaggccat
    31981 tattatggtc actcatgatg atcggatgct aaaatattgt gacaaagtct atcggatgca
    32041 agatggagag ttacgtcaag agagatagta gactggtaag agtttatgat attagaagaa
    32101 aaaaaccaaa caagcacagc acttgtttgg tttttttcta agtgtgacta ttatcaatac
    32161 gtcaccattt ccctaaagct tactatacgg gatgctaaag gtatcaccat gctcaaagtc
    32221 gccatattgg taacctcggt caaaccaacg ttggcgttgc ttagaagtgc catgtgtaaa
    32281 actatcagga acggttctgc cataggtttc ttcttgcaga gtatcatccc caactgcgtg
    32341 agctgctgcc attgcttcct caatatctcc tttttccaaa aggccttgcc cttgaacata
    32401 attagcccag gcaccagcat agtaatcagc ttgtaattcc aacttgacgt tgagctgatt
    32461 ggctttagcc tttgattttc cttgtctagc gctagcgtaa ttatccataa ttcccaattc
    32521 attttggatg tggtggccaa cttcatgagc aatcacatag gccatagcaa aatcaccttt
    32581 tgcgccgtac ttggttgata gttcattata aaaagaaata tctaaataca ctttttgatc
    32641 gcctgggcaa taaaaaggac cagaagaggc ttgacccctg ccacaagcgg tttgagttgc
    32701 tccagtatat agaactaatg ttggtttatg ataagttaac cctttctcac gaaatgtctt
    32761 agtccaatag tcctcggtag aggcgaagac cttactgaca aaagaaacct gttcttgact
    32821 agccttgtca ccatttgtac gcgttacttg gcttgattga taggcattgt tgttggtaga
    32881 agaaggcttg cctcctaaaa cactagatag gccaccccct cccataacta ggagaaggag
    32941 aagaataacc agcttggttt tccaacctcc tcgtgaaaag agtaattgaa gcaggagacc
    33001 gccccctagg ccgccgcccc caaaagaacc agacgattga ccacgtctat cttcaacctg
    33061 ttgactttct ctcaaatcat ccgttttcat aagtttctcc catctatttc ttgattttat
    33121 tataaaataa ttctattaga attgctattt ttatgatgag attataacta aaaataggaa
    33181 aagcaatagg tatttgctat aatataacaa gggtagattc agtttgtata aaggagtcat
    33241 atgaaattaa tttacacaga gatgagttac tcgatgacag agattttggt gaatgaagca
    33301 cgaaaagctg ctgaccaagg ttatcgagtg ttctatattg ctccaaattc tctttcattt
    33361 gaaaaggaaa gagaagtctt aaccttattg cctgaacgag ggactttttc gataatagta
    33421 acccggttcg ttcaaatgtc aaggtatttt acagttgaga gctctccttc taagcaacat
    33481 ctagatgaca cgactttggc tatgattttt tatcgggcct tgatgcaatt aaaaccagaa
    33541 gatttgccga gttatggtcg tttgcaaaat aattcggttt ttattgaaca actagttgag
    33601 ttatataagg aattaaaaaa cgcacaatta agcgtacatg atttaacagg attagaccat
    33661 cctcaaaaac aagaagatct tattaaaatt attgagcttg cagagacgat catgattcag
    33721 caagattata atcaagatag tcctctacaa tcttttgcaa gagcaattaa gttaggactt
    33781 ttaaataacc aactatcaaa aacagttatt gttattgatg gtttttcacg tttttcagct
    33841 gaagaggatt atttattatc acttttaaat aacaactgtc aagaggtcat cattggcagt
    33901 tatgtgagtc aaaaagctta tcaaaaatct tttattaagg gaaatatcta tgaggctagc
    33961 cttcattttt tgcaagattt ggctcaaaaa taccatatca agccggtatt tgcgacctct
    34021 aatcaagtgt ttaaaccagc cttttcacgg ctaacacaat tatttgaagc aactcatgat
    34081 ttttcacaag tagactggca actccaaaag aatgatcttg atcactttag cctttggcaa
    34141 tgtcatcatc aaaaagagga aattgaacat gtagcaaaat ccattcggca aaagctatat
    34201 gaaggttacc gttataagga tatcttagtg ttgctaggag atatggatgc ttatcaatta
    34261 caaattgggc ctatctttga taagtttgaa atcccctact atttaggaaa agcagagcct
    34321 atggctgccc atccattagt ccaatttata gaatcgctcg aacgaagtca gcgctataat
    34381 tggcgtcgtg aagacatttt aaacatgtta aaatcaggac tttttggttg ttttgatgat
    34441 agtgatattg atcgctttga ggaatatact cagtttgcag atattaaagg atttactaaa
    34501 ttttcaaagc cttttaccat taactcttct aggcagtatc ctcttgactt tttaaatgaa
    34561 atgagacaag acatcgtttt acctttgcaa gaattgttta agagtcaaaa acaacttggt
    34621 gcttcacttg ttgacaaatt aatacttttt tttaaaaaga ttagattagc tgagaatatg
    34681 caaggtttag cacagtccca attagaggtg gaaaaaaacg aagaagtttg gaaaaggttt
    34741 acagatattt tgacttcttt tcatcatatc tttggccaag aaaaattacg gctatcagat
    34801 tgccttgctt taatcaaaac gggaatgaaa tcagctcagt atcgtgttgt tccagcgaca
    34861 ttagatgttg tcactattaa atcttatgac cttgttcaac cacattcaaa acctttcgtt
    34921 tatgcaattg gtctaactca atcacatttt cctaagcaaa ttcaccattc aggactactt
    34981 tctgatcaag agcgtgctag aattaatgaa ataagaaatt atcgtcattt tgatattgct
    35041 agtgccgaaa atagtaaaaa aaaccatcaa actgcgttat ctctgtttaa tgctgctact
    35101 aaagagttag ttcttagtgt tccaacggta atcaacgaga cattcgatga tctatctcct
    35161 tatcttaaag aattaataag ctttgggcta cctcttttgg ataaaggcaa aaactatcta
    35221 tcttatgata atagcgatat tggtaactat aaagctctct tgtcacaaat tattgctatt
    35281 aaccgacaag atcttataga aatgtcagat caagataaga tgttttggac tgttgtctta
    35341 cgttatctca ggaaacagtt aagaaagcaa cagctagagc tacctacttc agattatcgt
    35401 ttatcaacaa agcctttgtc taaagaagtt atcgaggtat gttttccaaa gggaatccct
    35461 ttaaagctat ctgcaactgc tttaaccgtt ttttataata atcagtacaa ctattttttg
    35521 aaatatgttt tgaatttaaa caaaacagaa tccattcatc cagattccag aattcatggt
    35581 caatacttgc atcgtgtctt tgagcggtta atgaaagatc acacgcaaga accgtttgat
    35641 aataaattaa aacaagcaat ctaccatacc aatcaagaaa gtttttttca gcaggtttac
    35701 caagataatg ccgaggctga gtacagttta gctatactag aagatattgt tagatcaaca
    35761 gcacctatct tgcaacttaa ccaaaacata aaggtcattg atcaagaaaa gaactttcat
    35821 ctggatatgg gaaatgagat attagtccac gggatcattg atcgtattga ccagctgagc
    35881 gacggtagct tgggcattgt tgactataaa tcaagcgcga atcaatttga tattgggacc
    35941 ttttataatg ggttgagtcc acaactagtg acttacctcg cagccttaaa acagatcgca
    36001 ccgcacgata taaaccaatt atttggggct atgtaccttc atttgcaaga tcctaaattg
    36061 gatctagtaa catttaaaca aattgataac acattagtgg agagtatcta taaagcgttg
    36121 acctataaag gtattttttc agaagtcgaa aaagagcatc tatctactgg tgcttaccaa
    36181 accaaaaatg ctctttattc caatgacgaa ttggaaacac ttttaaatta taacaagtac
    36241 ctctatctta aagctgctaa acacattaaa aagggacatt ttttgattaa tccttataca
    36301 agcgatggta agacagttca aggagatcaa ttaaaagcca ttacccgttt tgaagcagat
    36361 cttgacatgg cacaggcaag gcgactagtg actctccctg ctaaggagaa aaaagagtgc
    36421 tttttaacat taatgagaaa ggagagccac ttgtgatttc ttttgcccca tttttaagcc
    36481 ccgaagctat taaacattta caagaaaacg aaaggtgcag tgatcagtct caaaaacgca
    36541 cagctcaaca aattgaagca atttatacta gtggccaaaa tatacttgta tcagcttctg
    36601 ctggttcagg aaaaaccttt gtaatggtcg aacgcatact tgataaaatt ttgagaggtg
    36661 tttcaattga tcggcttttt atctcaacct ttactgttaa agcagctaca gaactgcgtg
    36721 aacggattga aaacaaatta tactcacaaa ttgctcaaac tacagatttt caaatgaaag
    36781 tttatttaac agaacaattg caatctcttg gtcaagctga tattggtact atggatgctt
    36841 ttgcacagaa agtagtaagt cgctatggtt atagcattgg catttcatcc caatttcgta
    36901 tcatgcagga taaagcagaa caagatgttt taaagcaaga ggtgtttagc aaactcttta
    36961 gtgagtttat gaatcaaaaa gaggcaccgg tgtttagggc tcttgtgaaa aatttttctg
    37021 gtaactgtaa agacacttca gcttttagag agttagttta tacttgttat tcttttagcc
    37081 aatcgacaga aaatccaaaa atatggttgc aagaaaattt tctaagcgct gctaaaactt
    37141 accaaaggct tgaagatatc ccggatcatg atattgaact cttacttttg gcaatgcaag
    37201 acactgcaaa tcagctaaga gatgtgactg atatggaaga ttatgggcag ctgactaagg
    37261 caggtagccg atctgctaaa tacactaaac acttaacgat catagaaaag ttgtctgatt
    37321 gggtgcgtga ttttaaatgt ttgtatggaa aagccggatt ggatcggttg atcagagatg
    37381 tgacaggcct tataccatct gggaatgatg ttacagtctc gaaggtaaaa taccctgttt
    37441 ttaagacctt gcatcaaaaa ttaaaacaat ttaggcattt agaaacaatt ttaatgtatc
    37501 agaaagactg ttttccctta ttggaacagt tacaagattt tgtgcttgcg ttttcagaag
    37561 cttatttagc tgtcaaaata caagaaagtg cttttgaatt ttcagatatt acacactttg
    37621 caatcaaaat tttagaggaa aatacggata ttcgccaatt ctatcagcaa cactatcatg
    37681 aggtgatggt tgatgaatat caagataaca atcatatgca agagcgactc ctgaccttac
    37741 tatcgaacgg tcataatcgc tttatggtag gagatatcaa acaatcgatc tatcgatttc
    37801 ggcaagccga tcctcagatt tttaatcaaa agtttagaga ctatcaaaaa aaacctgagc
    37861 aggggaaagt gattttactc aaagaaaact ttcgtagcca atcagaggtg ttaaatgtca
    37921 gcaatgctgt ttttagtcac ttaatggacg aatcagtagg agacgtctta tacgatgagc
    37981 aacatcagtt aatagcaggt agtcatgctc aaacagtccc ctatctagac cgtcgtgctc
    38041 agttattgct atataatagc gataaagatg atggcaacgc cccttcagat agtgagggta
    38101 tttcatttag tgaggttaca attgttgcca aagaaattat taagcttcac aatgataagg
    38161 gtgtcccttt tgaagacatt acgttactcg tttcttcaag aacaagaaat gatatcattt
    38221 ctcatacatt caatcaatat agtattccta tagtaacaga tggtgggcag caaaactatc
    38281 ttaaatctgt tgaagtgatg gttatgttag atacattacg caccattaat aacccaagaa
    38341 atgattatgc ccttgtggct ttactgcgct caccgatgtt tgcctttgat gaggatgatt
    38401 tagcaagaat agcacttcaa aaagacaatg agctagataa agattgccta tatgacaaga
    38461 tacaaagggc tgtgattgga agaggtgctc atcctgaatt gattcacgat accttgcttg
    38521 gcaagttaaa tattttttta aagacgttga aaagttggcg tcgatacgct aagctagggt
    38581 cgttgtatga cttgatttgg aaaattttta atgatcgttt ttattttgat tttgtagcta
    38641 gtcaagcaaa agcagaacaa gcacaagcta atctatacgc attagctcta cgtgctaatc
    38701 agtttgaaaa atcgggctat aaagggctat accgttttat taaaatgatt gataaggtac
    38761 ttgagacgca aaatgactta gcagatgtgg aagtggctgc tcctaaacaa gctgttaatt
    38821 taatgaccat tcacaagtct aaaggtttac aatttccgta tgtatttatc cttaattgtg
    38881 acaagcgctt ctcaatgaca gatattcata aatcatttat tctgaatcgg cagcacggta
    38941 tcggtatcaa gtaccttgca gatatcaaag gtttacttgg tgaaacaaca ctcaattctg
    39001 ttaaagtaag catggaaacc ttaccttatc aattgaacaa acaagagttg cgcttagcaa
    39061 ctttatcaga acaaatgcgc ttattgtatg ttgctatgac acgagctgaa aaaaaagttt
    39121 attttattgg taaagctagt aagagcaaaa gtcaagatat cacagatcct aaaaagttag
    39181 gcaaactttt gccgctggct ttacgagaac agttattgac attccaagat tggctattag
    39241 caatagcaga tatattttca actgaagatc tttattttga tgttcgcttt attgaagata
    39301 gtgatttgac acaagagtca gtcggacgac ttcaaacacc acagttatta aatccagatg
    39361 atcttaaaga taatcgtcaa tcagaaacaa ttgcacgggc tttagatatg ttagaagcag
    39421 tgtctcaatt gaatgccaat tatgaagcag ctattcattt gccaacagtt cgaacgccta
    39481 gccaacttaa ggcagcttac gagcctttat tagaacccat tggtgtagat attatagaga
    39541 aatcttctcg atcgctatct gattttactt tgccacattt ttcaaaaaaa gtaaaagttg
    39601 aagcaagtca tattggatca gctcttcatc agttgatgca ggtgctccct ttgtcaaaac
    39661 cgataaatca acaaacgctt ttagacgctt taagagaaat tgatagtaac gaagaggtaa
    39721 aaacagctct tgatctcaaa aaaatagagt cgttcttttg tgatacaagc ctaggccaat
    39781 tttttcagac ttaccaaaaa cacttgtatc gagaagcacc atttgctatt ttaaaagttg
    39841 accctattag tcaagaagaa tatgtcctac gtggtattat agatgcctac tttttgtttg
    39901 atgatcatat tgtattagtg gactataaaa cagataaata caagcagccc attgagttaa
    39961 aaaagcgtta ccaacaacag ttggagttat atgcagaagc tctcactcaa acgtataaac
    40021 ttcctgtgac taagcgctat cttgttttaa tgggaggtgg aaagccagaa attgtcgaag
    40081 tttaaggttg ttgtgacaca agggtatttt gcaaatgata atagtatagt tgaggatttt
    40141 acgaaatgct tgaaaagcta ttgacctttg aataataata caatataata agttttatta
    40201 gtgacaaaaa ggaataaata tactatgaat tttaaaaaaa taagatttgg ttttttgctt
    40261 tgcctcctac tttatccttt agcagcttgt aataagtctg aacaacttaa tcactacgaa
    40321 agaattaaaa aaacaagaaa attagtcgtt gctgtaagcc ctgattatgc tccttttgaa
    40381 tttaaagctt tagttaatgg aaaagatact attgttggag cagatgttca attagctcag
    40441 gctattgctg atgaattaga cgttgatctt gagctttcac caatgagttt tgataatgtt
    40501 ttgtctagtt tacagactgg taaagctgat cttgctattt caggaatctc acatactaaa
    40561 gaaagagcta aagtatatga tttttcaatt ccttactatc aggcagaaaa cgctatagtg
    40621 atgagagcat ctgatgctaa agtgaccaaa aatattagtg acctaaacgg taagaaagta
    40681 gccgctcaaa aaggtagtat cgaggaaggt ttagttaaaa tacaattacc aaaggccaat
    40741 ctgatttctt tgactgctat gggagaagcc attaatgaac taaaagcagg acaagtctat
    40801 gcagttacct tagaagcacc tgtagctgct ggttttttag cccaacataa ggatctggct
    40861 ttagcaccct ttagcttaaa aaccagtgat ggagatgcca aagcagtggc ccttcctaaa
    40921 aatagtggtg acttaaccaa agccgttaat aaggttattg ctaagttaga tgagcaagaa
    40981 cggtacaagt catttattgc agagacaata gcactaacga aaaatactat gaagtaataa
    41041 ggttaataaa aactttatta gtat
//
LOCUS       DATXQK010000012        39813 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.012, whole genome shotgun sequence.
ACCESSION   DATXQK010000012 DATXQK010000000
VERSION     DATXQK010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 39813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39813
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.012"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..174
                     /locus_tag="VC394_000681"
     CDS             <1..174
                     /locus_tag="VC394_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rgg/GadR/MutR family transcriptional regulator"
                     /protein_id="HEP5994172.1"
                     /translation="FYEQTVFLYATGYFEFKRQLETGVEKMKQAIQVLDILNEDKLKL
                     HYADHFDKLVNKD"
     gene            complement(427..3966)
                     /gene="smc"
                     /locus_tag="VC394_000682"
     CDS             complement(427..3966)
                     /gene="smc"
                     /locus_tag="VC394_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEP5994173.1"
                     /translation="MFLKEIELEGFKSFADKTKIEFYKGVTAVVGPNGSGKSNITESL
                     RWALGESSAKNLRGGKMPDVIFAGTQNRNPLNYAKVAVVLDNSDHFIKTAKKEIRVER
                     HIYRNGDSDYLIDGRKVRLRDIHDLFMDTGLGRDSFSIISQGRVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRKKETQIKLNQTQDNLDRLEDIIYELDTQLAPLEKQAKVAKQFLEL
                     DANRKQLQLDILVKDIDIAQERQTKDTEALAALQQDLASYYAKRQSMEEDYQKFKQKK
                     QVLSQESDQTQTTLLELTKLIADLEKQIELVKLESGQEAEKKAEAKKHLEQLQEQLDG
                     FQAEEKQRTEQLLHIDQQLCDVKQQLNELSNALERFSSDPDQLMETLREEFVLLMQKE
                     AALSNQLTALKAHLDKEKQARQHKAQEYQLLVTKLDQLNDESQKAQAHYKAQKEQVEM
                     LLQNYQEGDKRVQELERDYQLKQERLFDLLNQKKGKEARKASLESIQKSHSQFYAGVR
                     AVLQSQKKLGGIIGAVSEHLSFDSDYQTALEIALGANSQHIIVTDEAAAKRAIAYLKK
                     NRQGRATFLPLTTIKARSLSEHYHRQLATCEGYLGTAESLVRYDDSLSAIIQNLLSST
                     AIFETIDQANIAARLLGYKVRIVTLDGTELRPGGSFSGGTNRQSNTTFIKPELEQISE
                     ELTRLVEQLKITEKEVAALQSDLIAKKEELTQLKLAGDQARLAEQRAQMAYQQLQEKQ
                     EDSKALLAALDQSQTTHSDESLLAEQARIEEALTAIAKKKSALTCDIDDIKENKDLVR
                     QKTQNIHQALSQARLQERDLLNEKKFEQANQSRLRTQLKQCQQNILKLESILNNNVSQ
                     DSIQRLPQWQKQLQDATEHKSGAQKRLVQLRFEIEDYEARLEETAEKITKESEKNDTF
                     IRRQTKLETHLEQVANRLRAYAKSLSEDFQMTLADAKEVTNSIDHLESAKEKLHHLQK
                     TIRALGPINSDAINQYEEVHERLTFLTSQKTDLTKAKNLLLETINSMDSEVKARFKVT
                     FEAIRKSFKETFTQMFGGGSADLVLTETDLLSAGIEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFAIIRVKTIPFVILDEVEAALDEANVKRFGDFLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGITMQESGVSKIVSVKLKEAQEMTN"
     gene            complement(3967..4659)
                     /locus_tag="VC394_000683"
     CDS             complement(3967..4659)
                     /locus_tag="VC394_000683"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEP5994174.1"
                     /translation="MKQLEELLSTSFDIQFNDLTLLETAFTHTSYANEHRLLNVSHNE
                     RLEFLGDAVLQLIISEYLFAKYPKKTEGDMSKLRSMIVREESLAGFSRFCSFDAYIKL
                     GKGEEKSGGRRRDTILGDLFEAFLGALLLDKGIDAVRRFLKQVMIPQVEKGNFERVRD
                     YKTCLQEFLQTKGDVAIDYQVISEKGPAHAKQFEVSIVVNGAVLSKGLGKSKKLAEQD
                     AAKNALAQLSEV"
     gene            complement(5092..5901)
                     /locus_tag="VC394_000684"
     CDS             complement(5092..5901)
                     /locus_tag="VC394_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEP5994175.1"
                     /translation="MNESGFKYSILASGSTGNCFYLETPKKRLLIDAGLTGKKITSLL
                     AEIDRKPEDLDAILITHEHSDHIKGVGVMARKYHLDIYANEKTWQLMDECNMLGKLDA
                     SQKHIFQRDKVLTFGDVDIESFGVSHDAIDPQFYRIMKDNKSFVMLTDTGYVSDRMTG
                     IIENADGYLIESNHDIEILRSGSYPWSLKQRILSDLGHLSNEDGAGAMIRSLGYNTKK
                     IYLGHLSKENNIKELAHMTMVNQLAMADLAVGTDFTVHDTSPDTACPLTDI"
     gene            complement(5905..7257)
                     /gene="vicK"
                     /locus_tag="VC394_000685"
     CDS             complement(5905..7257)
                     /gene="vicK"
                     /locus_tag="VC394_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEP5994176.1"
                     /translation="MTRDIIGNLSTFELAILILLVFVAFYFIHLAVRDYRNARIIRMM
                     SHKIRDLINGRYTDIIDEKADIELMELSDQLNDLSDVFRLTHENLAQEKNRLASILAY
                     MSDGVLATDRSGKIIMINETARKQLNLSKEEALKKNITDLLEGDTSYTYRDLVSKTPV
                     VTVNSRNDMGEFVSLRLRFALNRRESGFISGLVVVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALKEDIAPSFIKVSLDETNRMMRMISDLLNLSRIDNQVT
                     QLAVEMTNFTAFITSILNRFDLVKNQHTGTGKVYEIVRDYPITSVWIEIDNDKMTQVI
                     ENILNNAIKYSPDGGKITVRMKTTDTQLIISISDQGLGIPKTDLPLIFDRFYRVDKAR
                     SRAQGGTGLGLAIAKEIIKQHHGFIWAKSDYGKGSTFTIVLPYEKDAAIYEEWEEDVD
                     "
     gene            complement(7250..7960)
                     /locus_tag="VC394_000686"
     CDS             complement(7250..7960)
                     /locus_tag="VC394_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019321978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP5994177.1"
                     /translation="MKKILIVDDEKPISDIIKFNLTKEGYDIVTAFDGREAVTIFEEE
                     KPDLIILDLMLPELDGLEVAKEIRKTSHVPIIMLSAKDSEFDKVIGLEIGADDYVTKP
                     FSNRELLARVKAHLRRTETIETAVAEENASSGTQELTIGNLQILPDAFVAKKHGQEVE
                     LTHREFELLHHLANHMGQVMTREHLLEIVWGYDYFGDVRTVDVTVRRLREKIEDTPSR
                     PEYILTRRGVGYYMKSYD"
     gene            complement(8122..9156)
                     /locus_tag="VC394_000687"
     CDS             complement(8122..9156)
                     /locus_tag="VC394_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3114 domain-containing protein"
                     /protein_id="HEP5994178.1"
                     /translation="MCYNMVKKDEEKAMKQAILDRYQALKCYQNAGLSNQAFRAIAKE
                     PIIDNRLGSPTFWVIWPIEKENQSAKQLLTFLLDLVEMPFELSGQLHETQTLLTRFHP
                     SLLPDHMFWKELASLVDQAFPGKTLSQAGELEKRLHQFRYVISSQQAQSIRNHYKMIE
                     MTDAQALALFLRSKKGPCLWRQAPDYTLMDSARLHNKLRFEDNKVIFPSQEVSYNIKV
                     LLWFHTEFILDSTGFFLNEVDAEVVTEKGIVNGASFNYGTDGPRHWDLDVDPISRHDP
                     QFRRDTLKGFRSPKRVFRQWFRAQKDDFMFSYFNAKGLFAYHNKSSFARVKKSAKQFK
                     RQIHPIKGWF"
     gene            complement(9212..10459)
                     /locus_tag="VC394_000688"
     CDS             complement(9212..10459)
                     /locus_tag="VC394_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEP5994179.1"
                     /translation="MLTKLEYWAKQCPNKKAIVADQISLTYQELWQAVLIKDQTIKDS
                     VPYIISHSRYLNQLLSFLRGLKEGSCPIILHPNISGTFQQQIKHVDSELLKKADFAVL
                     SSGTTGKAKLFWRCLSTWTRLFDYQNKVFGMTGNSCLFLHGSFSFTGNLNLALAQLWA
                     GGCLVLSQKLSLKTWLSLWQAKKVSHLYLLPTYLNRLLPYLTKNNMTATHLLTSSQMI
                     SQELLRHYYKKFPQLEIVIFYGASELSFITWCNGRAAVKINGLVGQPFPDVSISFKDK
                     EIFVETPYSVEGMSQPYSVSDLGKMSPAGLILEGRQDDWVNQRGVKCHLPSLVELAHQ
                     APNVKEAHALKIGKGENETLILVLVLTKKDCLAPIKDFLALYLNSGQLPKYYLVIDCL
                     PLKDNGKINREVLLNKIPKQWLS"
     gene            complement(10416..11564)
                     /locus_tag="VC394_000689"
     CDS             complement(10416..11564)
                     /locus_tag="VC394_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEP5994180.1"
                     /translation="MTDVYIAAGLRTPIGLVGKQFAKEQPEILGAKLINALQNKYPVP
                     IDQVICGNAVGTGGNIGRLMTLYSHLGESVSALTVDMQCASAGAALSVGYAKIKAGMA
                     SNLLVGGIESSSLQPESVYASADWRQGAYKVAQFSPDSISPFAMIEGAERVAREHGFT
                     KEYLNHWTLRSHQKASYCQEQALLADLILDLPGASDQGIRPRLSSKVLSKVPPILGED
                     HVISAANACLTHDAAAFLQLSSQPSAFKLIDVVEVAGDPQRSPLMVIKASQVLLEKHG
                     LGMADMTAIEWNEAFAVIDGLFETHYPDLLDRYNIFGGALAYGHPYGASAAIIILHLM
                     RALEIKNGRYGIAAIAAAGGQGFAVLLKYHKEFSHADKARILGKAMPK"
     gene            complement(11959..12108)
                     /locus_tag="VC394_000690"
     CDS             complement(11959..12108)
                     /locus_tag="VC394_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase"
                     /protein_id="HEP5994181.1"
                     /translation="MQLLDNVYGTLRSFAFMRPVKSSNDTSLADALQADTDMAFILVR
                     GINYQ"
     gene            complement(12194..12979)
                     /locus_tag="VC394_000691"
     CDS             complement(12194..12979)
                     /locus_tag="VC394_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP5994182.1"
                     /translation="MAKLRRMHAIFIKQYLKQIMEYKVDFVVGVLGVFLTQGLNLLFL
                     SVLFQHIPSLEGWTFEQIAFIYGFSLIPKGIDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PISPLFHVLVETFQVDALGELLVGVILLVTTAGSIVWTLPKVLLFILVIPFATLIYTS
                     LKIATASISFWTKQSGAVIYIFYMFNDFSKYPMSIYHSFLRWLISFVIPFAFTAYYPA
                     SYFLTGQHLLFNIGGLVVVSLLVLALSLKLWKWGLDAYESAGS"
     gene            complement(12981..13799)
                     /locus_tag="VC394_000692"
     CDS             complement(12981..13799)
                     /locus_tag="VC394_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEP5994183.1"
                     /translation="MWSFWKRYRPFLSAGIQELITYRVNFFLYRIGDVMGAFVAYYLW
                     KAVFDSSKQSLINGFTLSDMTFYIIMSFVTTLLTKSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFMEIGFRWIVLMSVGFPFLMVLSGIKVMAGLSTLQVLASSCLYLVSLLLA
                     FLINFYFNICFGFSAFVFKNLWGSNLLKNALVAFMSGSLIPLAFFPKVVYIVLSFLPF
                     SSLVYTPVMIVIGKYSLSQIMVALSLQIFWLLVMVVLSQVIWKKVQYHLTIQGG"
     gene            complement(13801..14793)
                     /locus_tag="VC394_000693"
     CDS             complement(13801..14793)
                     /locus_tag="VC394_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEP5994184.1"
                     /translation="MVMIEVSHLQKNFSKTIKEPGLKGALKSFVHPQREIFEAVKDLS
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGYCRINGKIPQDNRQDYVRDIGAV
                     FGQRTQLWWDLALQETYVVLKEIYDVPEKAFRKRMDFLNEVLDLNEFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEKTTILLTTHDLS
                     DIEQLCDRIIMIDKGQEIFDGTVTQLKQSFGKMKSLSFELKPGQEQVVSQFMGLPDIT
                     VERHELSLDIQYDSSRYQTADIIQKTMADFAVRDLKMTDVDIEDIVRRFYRKEL"
     gene            complement(14934..16877)
                     /gene="thrS"
                     /locus_tag="VC394_000694"
     CDS             complement(14934..16877)
                     /gene="thrS"
                     /locus_tag="VC394_000694"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEP5994185.1"
                     /translation="MIKITFPDGAVREFESGVTTFDIAESISKSLAKKALAGKFNDQL
                     IDTTRAIEEDGSIEIVTPDHKDAYEVLRHSAAHLFAQAAKRLFPNLHLGVGPAIAEGF
                     YYDTDNAEGQISNEDLPRIEAEMQKIVTENYPCIREEVTKEEALELFKDDPYKVELIN
                     EHAGAGLTVYRQGEFVDLCRGPHVPSTGRIQVFHLLNVAGAYWRGNSDNNMMQRIYGT
                     AWFDKKDLKAYLTRLEEAKERDHRKLGKELDLFMISQEVGQGLPFWLPDGATIRRTLE
                     RYITDKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPVMDMGDGEEFVLRPMNC
                     PHHIQVYKNHVRSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHIFVTPEQ
                     IQEEFQRALQLIIDVYADFNLTDYRFRLSYRDPNDTRKYYDNDEMWENAQSMLKAALD
                     EMGVDYFEAEGEAAFYGPKLDIQVKTALGNEETLSTIQLDFLLPERFDLKYIGADGEE
                     HRPVMIHRGVISTMERFTAILIETYKGAFPTWLAPHQVTVIPISNEAHIDYAWEVAKT
                     LRDRGVRADVDDRNEKMQYKIRASQTSKIPYQLIVGDKEMEDKSVNVRRYGSKATHTE
                     SVEEFVENILADIARKSCPDAQA"
     gene            complement(17299..18633)
                     /locus_tag="VC394_000695"
     CDS             complement(17299..18633)
                     /locus_tag="VC394_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEP5994186.1"
                     /translation="MRVGLFTDTYFPQVSGVATSIRTLKEELEKEGHEVYIFTTTDRD
                     VKRFEDPTIIRLPSVPFVSFTDRRVVYRGLISSYKIAKHYNLDIIHTQTEFSLGLLGK
                     MIGKALRIPVVHTYHTQYEDYVSYIANGKIIRPSMVKPLLRGYLKDLDGVICPSRIVL
                     NLLEGYEVTIPKRVIPTGIPLEKYIRDDITAEEVTNLKAELGIAGDETMLLSLSRISY
                     EKNIQAIINQMPAILAENAKIKLIIVGDGPYLQDLKHLAMQLEVDKHVTFTGMVPHDK
                     VALYYKACDFFISASTSETQGLTYIESLASGTPIIAHGNPYLDDVVTDKMFGTLYYAE
                     TDLTDAIIDAILKTPVMDKRLLAKKRYEISAQHFGKSIYTFYLDTLIARNSKEAQKLS
                     LYLNHSGKSSSLKLVQGAIHLPKRAAKVTAITSVKVVKAPIKLVHAIKDFLD"
     gene            complement(18635..19633)
                     /locus_tag="VC394_000696"
     CDS             complement(18635..19633)
                     /locus_tag="VC394_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEP5994187.1"
                     /translation="MKVLLYLEAENYLRKSGIGRAIKHQAKALSLVGQHFTTNPRETY
                     DLVHLNTYGLKSWLLMIKAQKAGKKVIMHGHSTEEDFRNSFIFSNLLSPWFKKYLCHF
                     YNKADAIITPTLYSKSLIESYGVKSPIFAVSNGIDLEQYGADSKKEAAFRRYFDIKEG
                     EKVVMGAGLFFLRKGIDDFVKVAQAMPDVRFIWFGETNKWVIPAQVRQMVNGNHPKNL
                     IFPGYIKGDVYEGAMTGADAFFFPSREETEGIVVLEALASRQHLVLRDIPVYYGWVDQ
                     SSAELATDIPGFIEALKKVFSGASNKVEAGYKVAQSRRLETVGHALVDVYKKVMEL"
     gene            complement(19764..20765)
                     /gene="ccpA"
                     /locus_tag="VC394_000697"
     CDS             complement(19764..20765)
                     /gene="ccpA"
                     /locus_tag="VC394_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002264971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEP5994188.1"
                     /translation="MNTDDTITIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNIANSYFSILAKGIDDIAAMYKYNIVLASS
                     DEDDDKEVNVVNTLFAKQVDGIIFMGHHLTEKIRAEFSRSRTPVVLAGTVDLDHQLPS
                     VNIDYRAAVSNVVDILAENHKCIAFVSGPLIDDINGKVRLAGYKEGLKHNKLDFKEGL
                     VFEANYSYKEGFELAQRVINSGATAAYVAEDELAAGLLNGLFEAGKRVPEDFEIITSN
                     DSPVVQYTRPNLSSISQPVYDLGAVSMRMLTKIMNKEELEEKEILLNHGIKKRGTTK"
     gene            20939..22024
                     /locus_tag="VC394_000698"
     CDS             20939..22024
                     /locus_tag="VC394_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEP5994189.1"
                     /translation="MTKLDQIRLYLDQKGAELAIFSDPVTINYLTGFFCDPHERQLFL
                     FVYHDLAPVLFVPALEVARASQAISFPVFGYVDSENPWEKIKAVLPNTAAKTIYVEFD
                     HLNVNKFHGLQTIFSGQFNNLTPYVQGMRLVKSADEINKMMIAGQFADKAVQVGFDNI
                     SLDATETDVIAQIEFEMKKQGIHKMSFDTMVLTGNNAANPHGIPGTNNIENNALLLFD
                     LGVETLGYTSDMTRTVAVGQPDQFKIDIYNLCLEAQLAAIDFIKPGVTAAQVDAAARQ
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMAGNDLVLEEGMCFSVEPGIYIPGKVGVR
                     IEDCGHVTKNGFEVFTHTPKELLYFEG"
     gene            22073..22738
                     /locus_tag="VC394_000699"
     CDS             22073..22738
                     /locus_tag="VC394_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEP5994190.1"
                     /translation="MDQTIHHQIQQALHFRTAVRVYKEEKISDEDLALILDAAWLSPS
                     SVGLEGWRFVVLDNKPIKEEIKPFAWGAQYQLETASHFILLIAEKHARYDSPAIKNSL
                     LRRGIKEGDGLNSRLKLYESFQKEDMDMADNPRALFDWTAKQTYIALGNMMMTAALLG
                     IDTCPIEGFHYDKVNHILAKHNVIDLEKEGIASMLSLGYRLRDPKHAQVRKPKEEVIS
                     VVK"
     gene            22749..23126
                     /locus_tag="VC394_000700"
     CDS             22749..23126
                     /locus_tag="VC394_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEP5994191.1"
                     /translation="MKALHTCIRVKDLDQSVAFYTSAFPFKENYRKDFPDSQFTLVYL
                     ALEGESYELELTYNYGHGDYDLGNGYGHIALGSEHFEADHKKHRQAGFPVTDIKELAD
                     KSARYYFIQDPDGYKIEVIDLNN"
     gene            23271..24197
                     /locus_tag="VC394_000701"
     CDS             23271..24197
                     /locus_tag="VC394_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEP5994192.1"
                     /translation="MTLLSIIVPCFNEEANILPYFEEMHQLETSMTNQLAFEYIFIDD
                     GSKDNTLGILRELAARFPNVHYLSFSRHFGKEAGLLAGLKEAKGNYITVMDVDLQDPP
                     ELLPVMYAKLKEGYDIVGTRRQNRQGEPLIRSMCSNLFYGLIKHLSDTEMVNGVRDYR
                     LMTRQVVDSILELGEVNRFSKGIFSWVGYRITYLSFENQKRKYGKSRWHFWELLRYSL
                     DGFINFSEMPLTIATWTGTFSFLISIFAILFIIIRKILFGDPVSGWASTVSIILFMGG
                     IQLFCMGIIGKYISKIFLETKKRPLYIIKEKH"
     gene            24408..25091
                     /locus_tag="VC394_000702"
     CDS             24408..25091
                     /locus_tag="VC394_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEP5994193.1"
                     /translation="MEWIKLIGIVIIVLGFILKFDAIATVVVAGLVTALVSGISFIDF
                     LDILGKEFTNQRLLTIFFITLPLIGLSETYGLKHRATQLIQRVQALTVGRLLTLYLII
                     RELAGLFSIRLGGHPQFVRPLIQPMGEAAAKANIGEELTDAEKDDIKAMAAANENFGN
                     FFAQNTFVGAGGVLLIAGTLEQLGYDGNQAKIAFSSILIAIISIIIVAIYNYLFEKKM
                     ERQHQKGDN"
     gene            25091..26017
                     /locus_tag="VC394_000703"
     CDS             25091..26017
                     /locus_tag="VC394_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEP5994194.1"
                     /translation="MANIANLVLETIYILIGLQLFHTAYCTFKDKTNPVYFGTALFWG
                     LLGVTFVGGAFLPNKVIGFIVIVLALLTLFKQVRIGTLPAFNEQKAEESAHRIGNWIF
                     LPVMLMAMISLLLALILPDFSKSAIGIAGILATIAILIITKQKPSALLAENNRMNQQV
                     STSGILPQLLGALGAIFAAAGVGDVIASLIREIVPADSRFFGVLAYVLGMVIFTMIMG
                     NAFAAFTVITTGIGVPFVFALGADPIIAGALAMTAGFCGTLLTPMAANFNALPVALME
                     IKDRNAVIKKQAPIALVLIISHIALMYLLAYY"
     gene            26067..26714
                     /gene="pcp"
                     /locus_tag="VC394_000704"
     CDS             26067..26714
                     /gene="pcp"
                     /locus_tag="VC394_000704"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEP5994195.1"
                     /translation="MKILVTGFDPFGGEAINPALEAIKKLPATIHGAEIKCIEVPTVF
                     QKSADVLQQHIESFQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RADGKAAYFSTLPIKAMVAAIHQAGLPASVSNTAGTFVCNHLMYQALYLVDKYCPNAK
                     AGFMHIPFMMEQVVDKPNTAAMNLDDITRGIEAAIFAIVDFKDRSDLKRVGGATH"
     gene            complement(26831..27550)
                     /locus_tag="VC394_000705"
     CDS             complement(26831..27550)
                     /locus_tag="VC394_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HEP5994196.1"
                     /translation="MSIMITKGQVRLLRTYSYDSNLYTQGLEQLNNNHILLSAGRYGF
                     SKVGVYDLTQEIFSEKIAFPDTVFAEGLTVVEDYFWLLTYKEGVAYKFDKATCNCLGA
                     YPFEGDGWGLAYDKENQCLWMTSGNAFLQKRDPKDFALLDTVLVAIESVPISMLNELE
                     YVDGYLYANIWQTNTIVKLQPDSGKVVATYDISPLLKALNLDKSHYPDLNVLNGIAHL
                     DQQRFLITGKLYPLMLEVVLD"
     gene            complement(27556..28023)
                     /gene="smpB"
                     /locus_tag="VC394_000706"
     CDS             complement(27556..28023)
                     /gene="smpB"
                     /locus_tag="VC394_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEP5994197.1"
                     /translation="MAKGEGHILAQNKKARHDYHIVETVEAGIVLTGTEIKSVRAARI
                     QLKDGFAQIKNGEAWLVNVHIAPFEQGNIWNADPERTRKLLLKKREITHLANELKGSG
                     MTLVPLKVYLKDGFAKVLIGLAKGKHEYDKRETIKRRDQERDIKKQMKHYNAR"
     gene            complement(28026..30359)
                     /gene="rnr"
                     /locus_tag="VC394_000707"
     CDS             complement(28026..30359)
                     /gene="rnr"
                     /locus_tag="VC394_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEP5994198.1"
                     /translation="MMKERIMTYLKEHGKSNVNDLAAALEMAGAKHFPSLIKTISKME
                     SQSLLRFSDDGSLALRKEREKKKEPTVQGVFRANKAGFGFLHVDENEDDMFIGRNDVG
                     YAIDGDTVEVVVKKPADRLKGTAAEAKVVAIVDRSLKTAVGTFILDDDKPKYAGYIRS
                     KNQKIQQKIYIKKEPVVLKGTEIIKVDIDKYPTRGHDYFVASVRDIVGHQGDVGIDVL
                     EVLESMDIVSEFPAEVLAEANAISEAPTAKDLIGRVDLRQETTITIDGADAKDLDDAI
                     HIKLLDNGNYELGVHIADVSYYVTEGSALDKEAIARGTSVYVTDRVVPMLPERLSNGI
                     CSLNPNVDRLTQSALMEINSQGHVVNYQICQSVIKTTYRMTYSTVNDMIAGDEEALQE
                     FASIADDVTLMVALHRILEAMRSKRGALNFDTQEAKVIVNDKGMPVDVVLRQRGIAER
                     MIESFMLAANECVAEHFAKAKLPFIYRIHEEPKAEKLQQFIDYASTFGIHIQGTANKI
                     SQEALQAFMAKVEGQPGAEVLNMMLLRSMQQARYSEHNHGHYGLAAEYYTHFTSPIRR
                     YPDLLVHRMVREYSQPSQEKRDHFTQIIPELATSSSQLERRAIDAERVVEAMKKAEYM
                     AEYVGEEFDGIVASVVKFGFFVELPNTIEGLVHITSLPEYYHFNERTLSLQGEKSGKV
                     FKVGQPIRVKLVKADKETGDIDFEYLPSDFDVVEKIKMSDKASRRDRRKSSKSSKGTK
                     KKEPKEVAKAKTKGKTKKGSKKPFYKEQAKKKSRKRS"
     gene            complement(30453..30689)
                     /gene="secG"
                     /locus_tag="VC394_000708"
     CDS             complement(30453..30689)
                     /gene="secG"
                     /locus_tag="VC394_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEP5994199.1"
                     /translation="MYNLLLTILLVLSVLLVIAIFMQPQKNPSSNVFDSSGSEALFER
                     TKARGFEAFMQRFTAVLVFFWLAIALVLAILSSK"
     gene            complement(30735..30881)
                     /gene="rpmG"
                     /locus_tag="VC394_000709"
     CDS             complement(30735..30881)
                     /gene="rpmG"
                     /locus_tag="VC394_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEP5994200.1"
                     /translation="MRVKINLECSECGSNNYLTSKNKSSHPEKIKVPKYCPKERKVTL
                     HVET"
     gene            complement(30878..32071)
                     /locus_tag="VC394_000710"
     CDS             complement(30878..32071)
                     /locus_tag="VC394_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEP5994201.1"
                     /translation="MNWRQNLKVAWLGNFFTGASFSLVMPFMALYVENLGTPKELVEY
                     YAGLAVAVTALASALFAPVWGKLADRYGRKPMMLRASFVMTFTMGGLAIIPNVFWLLI
                     LRLLTGVFAGYVPNATALIASQAPKEESGYALGTLATGVTAGALIGPLLGGILAELLG
                     IRQVFLLVGVILFLCSLMTAVYVKEEFKPIRRFEMIPTKVILKQVKSPQIMLGLFVTS
                     MIIQISAQSVAPILSLYIRHLGQTHNLMFTSGLVVSAMGFSSLFSSSYLGKLGDRFGN
                     HRLLLAALCYSFIMYFSSALAQTSFQLGVLRFAYGFGVGALMPSINSLLTKLTPKEGI
                     SRVFAYNQMFSNLGQVIGPFIGSNVAVVLGYRSVFYVTSLIVFVNLIWSLIIFRKYIK
                     VKDIV"
     gene            complement(32193..33695)
                     /locus_tag="VC394_000711"
     CDS             complement(32193..33695)
                     /locus_tag="VC394_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HEP5994202.1"
                     /translation="MTTMTIAKGSQPIALQLGMLNRHGLIAGATGTGKTVTLKVLAEQ
                     LSLAGVPVFLADIKGDLSNLTKAGEVTDKLAARLATIGVSDYQPQAFPVRMWDVFGQN
                     GQPLRTTISEMGPMMLSRLLNLNDTQTGVLNIVFKIADEKGWLLIDLKDLQAILKEVG
                     DHASDYSSHYGNIAKQSIGTIQRSLLTLEQEGAHQFFGEPALDVADLMQLDVASGYGA
                     INILSATKLFQSPTLYTTFLLWLLSELYELLPEVGDLDKPKMVFFFDEAHLLFKDAPK
                     VFLEKVEQIVRLIRSKGVGIFFVTQNPLDLPETVLAQLGNRIQHALRAYTPKEQKAVR
                     VAADTFRQNPDLDVARVITELEVGEALISVLNDKGQPSIVERAYIMPPKSSFAVLSEI
                     ESQQLVQSSPFASKYSQSIDRESAYEKLAAKVLEDNRLAQEAIATAKREKEAKEAIKA
                     QAATKKASRRSVGRPRKTVVEKATDAFISTTVRTIGRELVRGLLGSLRKR"
     gene            complement(33885..34478)
                     /locus_tag="VC394_000712"
     CDS             complement(33885..34478)
                     /locus_tag="VC394_000712"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEP5994203.1"
                     /translation="MIIGITGGIASGKSTVVKVIRKAGYQVIDADQVVHDLQEKGGRL
                     YEALREAFGNQILKADGELDRTKLSEMLFSNPDNMATSSAIQNQIIKEELAAKRDHLA
                     QSQAIFFMDIPILMELGYQDWFDAIWLVYVDAQTQLQRLMARNRLDKGKARQRIASQL
                     PIEEKKPYASLVIDNSGDIAALIKQVQSALLSLANPR"
     gene            complement(34475..35302)
                     /gene="mutM"
                     /locus_tag="VC394_000713"
     CDS             complement(34475..35302)
                     /gene="mutM"
                     /locus_tag="VC394_000713"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEP5994204.1"
                     /translation="MPELPEVETVRRGLETLVLGQEIVAVTLKVPKMVKTDLETFALT
                     LPGQIIQSVGRRGKYLLIDLGQLVLVSHLRMEGKYLLFPDEVPDNKHFHVFFELKNGS
                     TLVYQDVRKFGTFDLIAKSQLSAFFAKRKLGPEPKKETFKLKTFEAALLSSQKPIKPH
                     LLDQTLVAGLGNIYVDEVLWAAKVHPGTASSRLNKAEIKRLHDETIRILALGIEKGGS
                     TVRTYRNALGADGTMQDYLQVYGQTGKPCPRCGQAIVKLKVGGRGTHICPKCQKKRP"
     gene            complement(35474..36340)
                     /gene="rgg2"
                     /locus_tag="VC394_000714"
     CDS             complement(35474..36340)
                     /gene="rgg2"
                     /locus_tag="VC394_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg2"
                     /protein_id="HEP5994205.1"
                     /translation="MEKELGKTLRRLRKGKQVSISFLADEYLSKSQISRFERGESEIT
                     CSRLLNLLDKLNITIDEFVSAHSKTHTHFFTLLSQARKCYAEKNVVKLTKLLKDYAHK
                     DYERTMIKAILFSIDSSIAPSQEELTRLTDYLFKVEQWGYYEIILLGNCSRFMNYNTL
                     FLLTKEMVASFAYSEQNKTNKMLVTQLSINCLIISIDHSCFEHSRYLINKIDLLLRDE
                     LNFYEKTVFLYVHGYYKLKQEEMSGEEDMRQALQIFKYLGEDSLYYSYKEHYRQIVLG
                     GKGDEDWSEADL"
     gene            36429..36497
                     /gene="shp2"
                     /locus_tag="VC394_000715"
     CDS             36429..36497
                     /gene="shp2"
                     /locus_tag="VC394_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone SHP2"
                     /protein_id="HEP5994206.1"
                     /translation="MKKVNKALLFTLIMDILIIVGG"
     gene            37411..37680
                     /gene="stcA"
                     /locus_tag="VC394_000716"
     CDS             37411..37680
                     /gene="stcA"
                     /locus_tag="VC394_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system protein StcA"
                     /protein_id="HEP5994207.1"
                     /translation="MTKKQFYLILAISILCFILAVSPKPPIPKHTIERKRTAMTIGHS
                     KQTPHKKEALKHKKNKNKALFLPSGDSAIKSVISMTVRLLLDDQY"
     gene            complement(38028..38426)
                     /locus_tag="VC394_000717"
     CDS             complement(38028..38426)
                     /locus_tag="VC394_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEP5994208.1"
                     /translation="MHRLPNRLIPEEDNNKLSRVAKEVVTEPRLNNPFLLPKKVLSLE
                     DDAAILNHLEEDRNVNKLFLEPVAAEAKREINFHPSDQQVTAKVKESSDFHQKQHQTG
                     ITYGDDTLADLTHHLLHSDGVIPSLNDKSA"
     gene            complement(38523..39593)
                     /locus_tag="VC394_000718"
     CDS             complement(38523..39593)
                     /locus_tag="VC394_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994209.1"
                     /translation="MMMLRDVKVKMSSLLVGCAATLLVSLSSTVAADSVHSSDRRFGV
                     GYKEETTSQFSSDQPSQVVLYRGEANTVRFTYTNQMYLMKDIRIALDGSGKSLTAQTV
                     PGMGHVYEGFQTSARGIFTMSGVPESTVPVANPNVQTKYTRYFKVIDDMHNTMYKGTV
                     FLVQPQAWKYTMKSVDQLPVDDLNHIGVAGIERMTTLIKNAGALLTTGSSGAFPDNIK
                     VSINPRGGQATIIYGDGSTDIIPSAVLWEKGSVKEPTEADQSVGTPTPGIPGKFKRDQ
                     SLNEHEAMVNVEPLSHVVKDNIKVIDEKSTGRFEPFRPNEDEKEKPASDVKVRPAEVG
                     SWLEPATALPSVEMSAEDRLKS"
BASE COUNT        11919 a         8528 c         7020 g        12346 t
ORIGIN      
        1 ttctatgaac agacagtttt tctctatgca acaggttact ttgaatttaa gcgccaatta
       61 gaaactggag ttgaaaagat gaaacaagcc atacaagttc ttgatatttt aaatgaagac
      121 aagttaaaat tacattacgc tgaccatttt gataaactag tcaacaaaga ttaagtgaaa
      181 tcattcttta ccttttaacg aaatgccaat caagagtgtc tcaataaaaa atcatctact
      241 ctgacaactt ttctctttta cctatcgaca gctaaaaagc cttggaaact gctatttcca
      301 aggctttctt tgtactctat tgtatcaatt agcttattcc gatttctgtt gctattagtt
      361 taatcatgaa atctcctaac ttatgaccga acatccaaaa aaacagaagg aactcccttc
      421 tgttttttaa ttggtcatct cttgagcttc ttttaatttt acagatacaa ttttagagac
      481 ccccgattcc tgcatggtaa ttccataaat actatctgct gcagccattg ttcctttacg
      541 atgggtcacg acaataaatt gactatcttt gtcaaagcga ttgaggaaat ccccaaagcg
      601 cttaacattt gcttcatcaa gagccgcttc tacctcatct aaaatcacaa acggaatcgt
      661 tttaacacgt ataatagcaa acaataaagc cagtgctgac aaagctttct ctccgcctga
      721 catcaagtta agagattgga ttttcttacc aggaggctgc acagaaatct ctataccagc
      781 agacaagaga tctgtttccg ttaaaaccaa atcagcagaa cccccaccaa acatctgagt
      841 aaaggtttcc ttaaaacttt ttcgaattgc ctcaaaagtc actttgaaac gagccttcac
      901 ttcgctatcc atactattaa tagtctctag caaaagattt ttagcttttg tcagatctgt
      961 cttttgactt gttaaaaaag tcaaacgctc atgcacttcc tcatactgat taatagcatc
     1021 actattaatt ggtcctagtg cacgaatagt cttttgtaaa tggtgtaatt tttctttggc
     1081 actttctaag tgatcaatac tatttgttac ctcctttgcg tctgctagtg tcatttgaaa
     1141 atcttctgat aaacttttag cataggctcg taagcgatta gccacttgct ctaaatgtgt
     1201 ctctaacttg gtttgacgac gaataaatgt gtcatttttt tcgctttctt tggttatttt
     1261 ttctgctgtt tcttctaagc gggcctcata gtcctctatt tcaaatctta attggactaa
     1321 acgtttttga gcaccagact tatgctctgt ggcatcttgt aactgttttt gccactgggg
     1381 tagtctttga atgctgtctt gacttacgtt gttgtttagt atgctttcaa gtttgagaat
     1441 attttgctgg cattgcttca gctgagttct tagacgagat tgattagctt gctcaaattt
     1501 cttttcatta agtaaatctc tttcttgcaa acgagcttga gataaagctt ggtgaatgtt
     1561 ttgtgttttt tgcctaacca aatctttgtt ttctttgata tcatcaatat cacaagttaa
     1621 ggcacttttt ttcttagcaa tagctgttaa tgcttcttca atacgagctt gctctgccaa
     1681 taaagattca tcagaatgag tcgtctgact ttgatcaagg gcagctaaca aagcctttga
     1741 atcttcttgt ttttcttgca attgctgata agccatttga gctctttgtt cagctaagcg
     1801 tgcctggtca ccggctaatt tcaattgtgt cagttcttct ttttttgcta tcaaatcgct
     1861 ttgcaaggca gctacttctt tttcagttat ttttaattgc tcaactaatc gcgttaattc
     1921 ctcactgatt tgttctaact ctggtttgat aaaagttgta ttgctttgtc tattagttcc
     1981 tcctgaaaaa gaaccacctg gtcgtaattc tgttccatca agagtaacaa ttcttacctt
     2041 atatcctaat aagcgcgctg caatattagc ttgatcaata gtttcaaaga tagcagtact
     2101 agataaaagg ttttggataa tagctgaaag actatcatca tagcgaacca atgattctgc
     2161 agttcccaga taaccttcac aagtcgctaa ttgacgatga tagtgttctg ataatgatct
     2221 agcctttata gtagttaaag gtaaaaaagt tgctcgtcct tggcgatttt ttttcaaata
     2281 agcaattgct ctttttgctg cagcttcgtc tgttacaata atatgctgac tattagcacc
     2341 taatgcaatt tcaagagctg tttggtaatc agaatcaaaa gaaaggtgtt cactgactgc
     2401 acctatgatc cctccaagct ttttttgtga ttgaaggaca gctcgtactc ccgcatagaa
     2461 ttgactatga cttttttgaa tagattctaa gctagcctta cgagcttctt ttcctttttt
     2521 ttgattcaac aagtcaaata gtctttcttg cttcagttgg taatctcttt ctaattcctg
     2581 aacgcgttta tcaccttctt gataattttg gagcaacatt tcgacttgct ctttttgagc
     2641 tttataatga gcttgagctt tttgagactc gtcattaagt tgatcaagct tagtaacaag
     2701 cagctgatat tcttgagcct tatgttgacg agcctgtttt tctttgtcta aatgggcttt
     2761 taaagctgtc agctgatttg ataaggccgc ctctttttgc atcaataata caaactcttc
     2821 tcttagagtt tccattaatt ggtcgggatc acttgaaaaa cgctctaatg cattgctcaa
     2881 ttcgtttaac tgttgtttga catcacaaag ttgttgatca atatgcaata attgttctgt
     2941 acgctgcttt tcttctgctt gaaaaccatc caattgttct tgtaactgtt cgaggtgttt
     3001 tttggcttct gcctttttct ctgcttcttg gccactttct agcttaacca attcaatttg
     3061 cttttccaag tcagctatta gcttggttaa ttctaataat gttgtttggg tttgatctga
     3121 ctcttgacta agaacctgct ttttttgctt gaatttttgg taatcttctt ccatagattg
     3181 acgcttagcg taataagatg ctaagtcttg ttgtaaagca gctaaagctt cagtatcttt
     3241 agtctgtctt tcttgagcta tatcaatatc tttgactaga atatctagtt gtagttgctt
     3301 acgattggca tcaagttcta aaaattgttt agctacttta gcttgctttt caagtggggc
     3361 caactgcgtg tctagctcat aaataatatc ctctagacgg tctaaattat cttgagtttg
     3421 atttaattta atctgagttt cttttttacg tgttttatat tttaaaacac cagctgcttc
     3481 ttcaaaaatg gcgcgccttt cttcaggttt actattgaaa atttcctcta cgcgaccttg
     3541 agaaataatg gagaaagaat cacgaccaag ccctgtatcc ataaacaaat catgaatatc
     3601 acggagcctt accttgcgac catcaatcag atagtcacta tctccatttc ggtaaatatg
     3661 cctttcgaca cggatttctt tcttggcagt ttttataaaa tgatcggaat tatcaagaac
     3721 aaccgctacc ttagcatagt ttagaggatt acggttttgt gtcccagcaa aaatgacatc
     3781 tggcatcttg cccccacgca gatttttagc acttgattct cctaaagccc aacgtaaact
     3841 ttctgtaata ttacttttac ctgaaccatt tggtcctacg actgcagtta ctcccttata
     3901 aaattcaatt ttagtcttgt ccgcaaagga cttgaaccct tccaactcaa tttcttttaa
     3961 aaacatctaa acctcactaa gttgggcaag ggcattttta gcagcatctt gctccgctaa
     4021 ttttttagac ttacctaaac ctttactaag gacagcacca ttaacaacta tcgatacctc
     4081 aaattgttta gcgtgggcag gtcctttctc actaatgact tgataatcaa tagccacatc
     4141 accttttgtt tgcaaaaact cttgaagaca agttttatag tctctaaccc tttcaaaatt
     4201 ccctttttcc acctgaggga tcatgacttg tttcaagaag cgacgtactg catcaatccc
     4261 cttatctaat aaaagagcac ctaaaaaggc ttcaaataag tctcctaaaa tagtatcacg
     4321 acggcgacca cctgactttt cctcaccttt tcctagtttg atataagcgt caaaagaaca
     4381 aaatcgtgaa aaacctgcca agctttcctc acgtacaatc atggaacgta acttagacat
     4441 atctccttct gtctttttag gatactttgc aaataaatac tctgaaataa tcaattgtag
     4501 aacagcgtct cctaaaaatt ccaagcgttc attatgtgaa acgtttagga gacgatgctc
     4561 gttagcgtaa gaggtgtggg taaaagctgt ttctagtaaa gtcaaatcat taaattgaat
     4621 atcaaaagat gttgagagta actcttcaag ctgtttcata aaaaatccct ttcatatttg
     4681 tcagtagacc ttatttagta taccaaaaat ccacctagaa aaccgactct aagtggattt
     4741 ttcgtgattt attaaacatc ttaaataccc gtcaaaggaa actttctcat acaatcaaaa
     4801 gactctacac tatctgaggg gaatttccca ctacagatag tatagagcta ttttgttttt
     4861 agtctgaaca actaaaaaac taccttatct ttataatcct caacaccttt gataaataga
     4921 tcagacaagc caaaactaca ggctaattta agtctattga tgctctaaag gatgaccaat
     4981 gagagttcat tgaactcaga agacaatacg ctttaagaca tcaaaaaaat agccctgact
     5041 ggtatatcac caatcagcgc tactttgcat attctctttt agtttcttat ctcaaatatc
     5101 agttaatgga caagcagtat ctggagaggt atcatggacc gtaaagtctg tacctactgc
     5161 taaatctgcc atagccagtt gattgaccat cgtcatatgc gctaactctt tgatgttatt
     5221 ttctttactc agatgaccta ggtatatttt tttggtattg taccccaggc ttctaatcat
     5281 ggctcccgct ccatcttcat ttgataaatg tcctaaatca gataaaatac gctgcttcaa
     5341 gctccaagga taagagcctg atctcaaaat ttcaatatca tgattggatt caatcaagta
     5401 gccatctgca ttttcaataa tacctgtcat cctatcactg acatatcctg tatctgtcaa
     5461 catcacaaaa gacttattat ctttcataat ccgataaaat tgaggatcaa tagcgtcatg
     5521 gctgactcca aaactttcga tatcaacatc tccaaaggtc aaaactttat ccctttggaa
     5581 aatatgtttt tgtgaggcat caagcttacc tagcatattg cactcatcca tcagctgcca
     5641 tgttttttca ttagcataga tatccaaatg gtatttgcgg gccataacgc ctacaccctt
     5701 aatatggtca gagtgctcat gggtaatcaa aatagcatcc aaatcctcag gtttgcggtc
     5761 aatttcagca agaagactgg taattttctt accagtaagc cctgcatcaa tcaataagcg
     5821 ttttttaggt gtttctaaat aaaaacaatt tcctgtggac ccagatgcta aaatactata
     5881 tttaaaacca ctctcattca ttgattagtc tacatcttcc tcccattctt catagatggc
     5941 tgcatctttt tcataaggca agacaatagt aaaggtcgat cctttaccat agtcactctt
     6001 agcccagata aagccatggt gctgcttgat gatttcttta gcaatggcaa ggcctagacc
     6061 ggtccctcct tgggcacgac ttcttgcctt gtctacacga tagaaccgat caaaaataag
     6121 aggcaaatct gttttaggga tacctagtcc ttggtctgaa atggaaataa ttaattgggt
     6181 atctgttgtt ttcatacgga ctgtgatttt tccaccatct ggagaatact taatggcatt
     6241 gttcaaaata ttctcgataa cctgtgtcat tttatcatta tcaatttcaa tccagacaga
     6301 ggtaataggg taatctctta caatttcata gacttttcct gtacctgtat gctgattttt
     6361 aaccaaatca aatctgttta aaatagaagt tataaaagca gtaaaattag tcatctctac
     6421 tgctaattgg gttacttgat tatcaatccg agacaggttt aaaagatctg aaatcatacg
     6481 catcatccga ttagtttcat caagagaaac ttttatgaaa cttggagcaa tatcttcttt
     6541 aagtgcacct tcatcaagag cctccaagta ggatttaacc gaagttaaag gggtccttaa
     6601 ttcatgactt acattagaga caaaaagacg gcgttcacgt tcttcttttt cttgttctgt
     6661 ggtgtcatgg agcaccacaa ccaagcccga aataaaacca ctctctctcc tattcaacgc
     6721 aaagcgcaag cgtaatgaga caaactcacc catatcattt cggctattaa cagttaccac
     6781 tggtgtttta gataccaaat cacggtaggt atatgaagta tcaccttcta acaaatctgt
     6841 aatgtttttc tttagtgcct cttctttact taaatttaat tgcttgcgag ctgtctcgtt
     6901 aatcatgatg attttaccag accggtctgt agcaagtaca ccatctgaca tataagccaa
     6961 aatacttgcc aagcgatttt tttcttgggc aagattttca tgcgtcaagc gaaaaacatc
     7021 tgataggtca tttaactggt ctgaaagctc cattaactca atgtctgctt tttcgtcgat
     7081 tatatcagta tagcgaccat taatcaagtc tcggatttta tggctcatca tccgaataat
     7141 acgtgcattt cggtaatcac gcaccgcaag atggataaag taaaaagcaa caaaaacaag
     7201 taagataagg atcgctaatt caaatgtgga taagtttcca atgatatctc tagtcataag
     7261 atttcatgta gtacccaaca ccacgtcttg ttaaaatata ctcaggacga cttggtgtgt
     7321 cttcaatttt ttcacggaga cgacgaacag tcacatcaac cgtgcgcaca tcgccaaaat
     7381 aatcatatcc ccaaacaatt tccaataagt gttctcgtgt catcacctga cccatatggt
     7441 tagctagatg atgcaatagt tcaaattcac gatgggtcaa ctctacctct tgaccatgtt
     7501 ttttagcaac aaacgcatct ggtaaaatct gtaaattacc aatggttagt tcctgtgtac
     7561 ctgaagaagc attttcttct gcaacagccg tttcaatagt ttcggtacga cgcagatgag
     7621 ccttgacacg tgccagcaat tcccgattag aaaagggctt ggtcacgtaa tcatcagccc
     7681 caatttcaag tccaataacc ttgtcaaact cactatcttt agccgacaac ataataatcg
     7741 ggacatgact ggttttacga atttccttgg ctacttcaag accgtccaac tcagggagca
     7801 tcaaatcaag aataattaaa tctggctttt cttcttcaaa aattgttacc gcttcgcgtc
     7861 catcaaaagc tgtaacaatg tcataacctt cttttgtcaa attaaactta ataatgtcag
     7921 aaatcggttt ttcatcatcc acaataagta tttttttcat gtctatacct cagaaagttc
     7981 ttgtaattga tattacaatt tgtgattttt gtggttaata aaaaatcaca aaactatatt
     8041 tttctaaatt atttctacct gattatacca aaaattcagc ctaattgaca atgacgacta
     8101 ccgttacaaa tagagtactt tctaaaacca accctttata ggatgaattt gacgtttgaa
     8161 ttgcttagca gattttttga ctctggcaaa actactcttg ttgtggtaag caaaaagtcc
     8221 tttggcatta aaataagaga acataaagtc atctttttga gctctaaacc actgacgaaa
     8281 tactcttttt ggcgatcgaa atcctttaag ggtgtccctt ctaaactgag gatcgtgacg
     8341 gctaatcgga tcaacatcta aatcccaatg tctaggacca tctgtgccat agttaaaaga
     8401 tgcaccattg acaattcctt tttcggtcac tacttctgca tcaacttcat taagaaaaaa
     8461 gcctgtgcta tctagaataa attcggtatg aaaccaaaga agtactttta tattatagga
     8521 aacttcttga cttggaaaaa tgactttatt atcttcaaat cttagtttat tatgtaacct
     8581 ggcagaatcc atcaaggtat aatctggtgc ttgccgccac aaacaaggcc cttttttaga
     8641 cctcaaaaaa agggccagag cttgggcgtc tgtcatctca atcattttat aatggtttct
     8701 gatagattga gcttgttgac tagagatgac ataacgaaat tgatgcaaac gcttttcaag
     8761 ctcacctgct tggcttagtg ttttgccagg aaaagcttga tcaactaagc tagccaactc
     8821 tttccaaaac atatgatctg gcaatagaga tggatggaat cgggtgagta gcgtttgagt
     8881 ttcatgcaac tgaccactta gctcaaatgg catctctacc aaatcaagaa gaaacgttag
     8941 taactgctta gctgattggt tttctttttc aataggccat attacccaga aagttggaga
     9001 accaagtcga ttatcaatga taggctcttt agctatagca cgaaatgctt ggtttgataa
     9061 acctgcgttt tgatagcatt tcaaggcctg atagcgatct aagatagctt gtttcatggc
     9121 cttctcctca tcttttttga ccatattata acacatctaa agctgaatcg tcacttgtat
     9181 ctcactataa gcaaaacgaa acctgaagtg gttaactaag ccactgtttg ggtattttat
     9241 ttaaaagaac ttctcgatta attttaccat tatcttttaa aggcaaacaa tcaatcacaa
     9301 gatagtactt gggaagttgt ccagagttta aataaagagc taaaaagtct ttaataggtg
     9361 ctaggcaatc cttcttagtc aaaactaata ctaatattag ggtttcgttt tctccttttc
     9421 ctatctttaa agcgtgcgct tctttgacgt ttggcgcttg gtgagcgagt tcaactaggc
     9481 taggcaggtg gcatttcact cctcgttgat taacccagtc atcttgtcgc ccttctaaaa
     9541 ttaaacccgc tgggctcatt tttcctaagt ctgagacact gtatggttgt gacattcctt
     9601 ctacactata aggagtctct acaaaaattt ccttatcttt gaaactgata gacacatcag
     9661 gaaagggctg tcctaccaaa ccattaattt tgacagcagc tctgccatta caccacgtta
     9721 taaaagataa ttcactagcc ccgtagaaaa tgacaatttc aagctgagga aattttttgt
     9781 aataatgtct tagtaattct tgtgatatca tttgcgaaga ggtcaataag tgagtcgcag
     9841 tcatgttgtt cttagtcaaa taaggtagca agcgatttag gtaggtcggt aaaaggtaaa
     9901 ggtgactcac cttttttgct tgccataagc ttaaccacgt tttcaaagac agcttttggc
     9961 ttaaaaccag acatcccccg gcccatagtt gagctaatgc taagttaaga ttgccagtaa
    10021 aactaaaact accatgtaag aatagacaac tattaccagt cataccaaac accttatttt
    10081 gataatcaaa aagtctcgtc caagttgata gacaacgcca aaataatttt gctttgccag
    10141 ttgttcccga actcaaaaca gcaaaatcag cttttttaag aagctcacta tcgacgtgtt
    10201 ttatttgctg ctgaaatgta cctgagatat tagggtgtaa aatgatagga caactgcctt
    10261 cttttaatcc tcgtaaaaaa gagagtaact gattaagata gcgactatga ctgatgatat
    10321 atggtacact gtcttttatt gtctgatcct ttattaaaac cgcttgccat agctcttgat
    10381 aagtcaagct tatctgatcg gctacgatag cttttttatt tgggcattgc tttgcccaat
    10441 attctagctt tgtcagcatg actaaactcc ttatgatatt ttagtaaaac agcaaaccct
    10501 tggccaccag ctgctgctat agcagctatt ccatagcgtc catttttaat ctctaaagcc
    10561 ctcattaagt gcaatattat aatggctgct gaggcaccat agggatgacc ataagctaat
    10621 gcaccaccaa atatattata gcggtccaat aaatctggat aatgggtttc aaatagacca
    10681 tcaataacgg caaaggcttc gttccattca atcgctgtca tatctgccat ccctagacca
    10741 tgtttttcca aaaggacttg actagcctta atcaccatta aagggctacg ctgtgggtct
    10801 cctgctactt ctaccacatc aatcagttta aaagctgaag gctgactact tagctgtaaa
    10861 aaggcagcag catcatgggt taggcaggca ttggctgcac taatcacgtg atcttctcct
    10921 aagatagggg gtacctttga caacacctta cttgacaagc gcggtcgaat accttgatca
    10981 ctggctcctg gcaggtctag aataagatca gctaacaggg cttgctcctg gcaataacta
    11041 gccttttgat gacttctcaa tgtccagtga ttcaaatact cctttgtaaa gccatgctct
    11101 cttgccactc gttctgcccc ttcaatcatc gcaaaagggc tgatactatc tggagaaaac
    11161 tgagctacct tgtaggctcc ttggcgccaa tcagctgaag catataccga ttcaggttgt
    11221 aaggagctgc tttcaatccc tcccactaaa aggttgcttg ccatacctgc cttgatttta
    11281 gcataaccca cggaaagagc tgcaccagct gatgcacact gcatatcaac cgtcaaagca
    11341 gagacagatt ctcctaaatg agaatacagg gtcatcaggc gcccaatatt accacccgtt
    11401 ccgacggcat tgccacaaat cacttggtca atgggaactg gatacttatt ttgtaaagca
    11461 ttgatgagct ttgctccaag gatttctggt tgttctttag caaattgttt ccctactaaa
    11521 ccgatagggg ttctaaggcc tgcagcaata taaacatccg tcattatatt gacctccttt
    11581 attttcgatg tccttaaatg gttattatat aattatttct gatggttttc ttttttgaac
    11641 taataactgc taaaaataca cctaaattaa tttagagaat ctctttagtc tcgctagtgt
    11701 gccatcaata aaatctataa aattctctaa cgtcattgaa atgtcaagtc ccacaactat
    11761 tgtcgtagaa gtcacttact ttgttgcttt aacattcaaa ctgtaacctt tgtttttgat
    11821 acagggtgac tttattatac tggaaaacca ttcctaagaa aagaaaatct tatatagaat
    11881 aaagaattag acaccataaa aaagctaagc tgtgacactt aactttttta tggtattgac
    11941 aaaccttgtc tttactattt attgatagtt aatgcctcta actaaaataa aagccatgtc
    12001 tgtatcagcc tgcaaggcat ctgctaagct ggtatcgtta ctagatttga caggtctcat
    12061 aaaggcaaaa gatctcaaag ttccataaac gttatctagc aattgcatgc aaccacttga
    12121 aatatatctg cttatgttat tttaccgccc ttaatttaga ccaatctgct gatgatggtt
    12181 ccaaacaatg cctctaggaa cctgcacttt cataagcatc taggccccat ttccaaagct
    12241 taagcgacaa ggccaaaacc agtaacgaca ccactaccaa gcctccaata ttaaagagca
    12301 ggtgctgacc tgttaaaaaa taagaagcag ggtaataagc tgtaaaagca aagggaatga
    12361 caaaactgat taaccaccgc aaaaaggaat ggtaaatgga catagggtac ttggaaaagt
    12421 cattaaacat gtaaaaaata taaataactg ccccagactg cttggtccaa aaagaaatgc
    12481 tggcggtcgc aatttttaaa gaggtgtaaa tcaaggtcgc aaaaggaata accaaaataa
    12541 acaaaagcac cttaggaaga gtccatacga tactaccagc cgtggtcact aataagataa
    12601 ccccaaccaa taattcaccc agggcatcca cttgaaatgt ctcaaccaaa acatgaaaca
    12661 aaggactgat tggtctggtg aggtacttgt caaattctcc tttgcggaca agccgttgcc
    12721 ctaaagccca aagattgtca aaaaagaggt gatcaattcc ctttggaatc aaagaaaagc
    12781 cataaataaa ggcaatttgt tcaaacgtcc agccttctaa agaagggata tgttggaaaa
    12841 gtaccgataa aaagagcaga ttaagtccct gagtgagaaa aacacctagc actcccacca
    12901 caaaatcaac cttgtattcc ataatttgtt tgaggtattg tttgataaaa atagcgtgca
    12961 tacgtctcaa ttttgccatc tcaccctcct tgaatcgtta aatggtactg aacctttttc
    13021 cagatgactt gcgataagac taccatcacc agtaaccaaa agatttgcaa acttagggcc
    13081 accattatct gactaaggct gtatttccca atgacaatca taactggcgt gtaaaccaaa
    13141 gaggaaaacg gtagaaaact aagcactata taaaccactt taggaaaaaa agccaatgga
    13201 atcaaactgc ctgacataaa ggcaaccaag gcatttttca ataaattaga cccccaaagg
    13261 tttttaaaga caaatgctga aaagccaaaa caaatgttga aataaaaatt gattaaaaaa
    13321 gctaataaaa ggctaactaa atacagacaa ctgctggcta ggacttgcaa ggtagacagc
    13381 cccgccatga ctttaatacc agataagacc attaaaaaag gaaagccgac actcattagc
    13441 acaatccagc gaaaaccaat ttccataaag aggtaagagg ctgcaaaatg aactggtcga
    13501 agcaggcgca tgatgataga accatctttc acttcttcgc caatcataaa ggagctgtct
    13561 gatttggtca gtaaagtggt cacaaaactc atgataatgt aaaaggtcat gtcagacaga
    13621 gtaaaaccat tgattaagga ttgcttagac gaatcaaaaa cagccttcca gaggtaataa
    13681 gccacaaaag cccccatgac atctccaata cgatacaaaa agaaattaac gcgataggtt
    13741 attaattcct gaattcctgc actgagaaag ggtctataac gtttccaaaa tgaccacatc
    13801 ttaaagctcc tttcggtaga aacgacgaac aatatcctca atatctacat cagtcatctt
    13861 caaatccctg accgcaaaat cagccatggt tttttgaata atgtccgctg tttgatagcg
    13921 cgaactgtca tattgaatgt ctaaactaag ctcatgccgt tccactgtga tgtcaggtaa
    13981 gcccataaac tgtgagacca cctgctcctg ccctggcttc aactcaaaag acagactctt
    14041 catttttcca aagctttgct ttaattgggt caccgttcca tcaaagattt cttgcccttt
    14101 atcaatcatg ataatccgat cacagagctg ttcaatatca ctcaaatcat gggtcgtcag
    14161 taaaatagtt gtcttttctt cttgattaat ttgagtaatg gcacgccgaa tgttgtcctt
    14221 cacagaaaca tcaagtccaa tcgttggttc atctaaaaac aatactttag gattatgcaa
    14281 caaggaagca gcaatatcag ctcgcatccg ctgaccgagt gataaggtcc ggacaggatc
    14341 cttaataaac tcattcaggt ccaaaacctc atttaaaaaa tccatgcgct tgcgaaaggc
    14401 tttttcaggc acatcgtaaa tttcttttaa aacgacatag gtctcttgca aggccaaatc
    14461 ccaccatagt tgtgttcgtt gaccaaaaac agctccaatg tctctcacat aatcttgacg
    14521 attatcttgt ggaatctttc cgttaatgcg acagtaacct gatgtaggct ttaaaatacc
    14581 tgttaacatc ttaatcgttg ttgattttcc tgcaccattg gctccaataa aacctaaaat
    14641 ttgccctttg ggtacctcaa aagataagtc cttaaccgcc tcaaagattt ctctttgggg
    14701 atgaacaaag gacttcaagg ctccctttaa tcctggctct ttaatggttt tggaaaaatt
    14761 tttttgaagg tgggacactt ctatcatcac catttaccat tcctcgtttc tgttatcgtc
    14821 ttatcaccgt tactaaacaa tttattataa caccatgaaa aagactgtgc aaccgtttgc
    14881 ttaatttttt aagaaagaaa aagaagccca taaggacctc tttttgccat tgattaagct
    14941 tgtgcatctg gacatgactt gcgagcaatg tctgccaaga tgttttccac aaattcttcc
    15001 acagattcag tgtgagtcgc cttactacca tagcggcgaa cattgactga tttgtcttcc
    15061 atttctttgt ccccaacaat taattggtaa ggaattttgc tggtttgaga cgcacgaatc
    15121 ttgtactgca ttttttcatt gcggtcatcg acatctgcac gaacaccacg gtcacgaagg
    15181 gtttttgcta cttcccaagc atagtcaatg tgggcttcat ttgaaattgg aatcacggtt
    15241 acttggtgag gtgccaacca agttgggaaa gcacccttgt aagtttcaat aaggatagca
    15301 gtgaaacgtt ccatagttga gataacacca cggtgaatca tgactgggcg gtgttcttcg
    15361 ccatctgcac caatgtattt aaggtcaaag cgttctggca acaagaagtc caactgaatg
    15421 gttgataggg tttcttcgtt accaagagct gttttcacct gaatatcaag cttaggccca
    15481 taaaaggctg cttcaccttc tgcttcaaag taatctacac ccatctcatc aagtgcagct
    15541 ttaagcatac tttgagcatt ttcccacatc tcatcattgt cataatattt acgagtatcg
    15601 ttagggtctc ggtatgacaa acgaaaacga taatcagtca agttgaagtc agcataaaca
    15661 tcaatgatta attgaagggc acgttgaaat tcttcctgga tttgctctgg tgttacgaaa
    15721 atgtggccat cattcaaggt catttcacgt acccgttgaa gtcctgatag ggcacctgat
    15781 ttttcgtaac ggtgcatcat gccaagctcg gcaatacgaa taggcaattc acgataagaa
    15841 cgcacatgat ttttatacac ttgaatgtgg tgtgggcagt tcattggacg aagaacaaac
    15901 tcctcaccat ctcccatatc cattacaggg aacatatctt cttgataatg atcccaatga
    15961 cctgatgtct tgtacaattc tacagaagca agtggtggtg tgtaaacgtg ttggtagcct
    16021 gatgctaatt ctttatctgt gatgtagcgc tcaagtgtac gacgaatagt cgctccatct
    16081 ggtaaccaaa acggcaagcc ttgaccaact tcttggctaa tcatgaacaa gtcaagctct
    16141 ttaccaagct tacggtgatc acgttctttg gcttcttcta gacgagtcaa gtaagctttg
    16201 aggtcttttt tatcaaacca agctgttcca tagatacgtt gcatcatgtt attgtcgctg
    16261 ttaccacgcc agtaagcacc cgccacattt agcaagtgga agacttggat acggcctgtt
    16321 gatggcacat gagggccacg gcaaagatcc acaaattcac cttgacggta aaccgtcaag
    16381 ccagcaccag catgctcatt aatcaattct accttgtaag gatcatcttt gaacaattcc
    16441 aaagcttctt ctttagtcac ttcttcacgg atacatgggt aattttctgt cacgattttt
    16501 tgcatttcag cttcgatacg tggcaaatct tcgtttgaaa tttggccttc agcattatct
    16561 gtatcatagt aaaagccttc tgcaatggca gggcccacac ccaaatgaag atttgggaac
    16621 aagcgttttg cagcttgggc aaataaatgg gctgcagagt ggcgaagcac ctcataagca
    16681 tctttgtggt caggtgtcac gatttcaata ctgccatctt cttcaatggc acgagtggtg
    16741 tcaatgagtt gatcattgaa cttaccagct aaagcttttt tagccaatga tttgctgatg
    16801 gactcagcaa tgtcaaacgt tgttacacca gattcaaatt cacgcacggc gccatctgga
    16861 aaagtaatct taatcatttg ctttctcctt taagtcagtt aagatttcca ctaaaacatg
    16921 gatagtaaga caaattaggg acaagaggtt agtgaatagc tcaataccac cacgtagaag
    16981 aacaatacct tattcacgaa tctaccctcc ctagtcctta aaaacaaggc taatcccctc
    17041 caaaacaaaa aaactgaccg catcccaaag gacgcaatca gcgtggttcc accttaattt
    17101 atgcagggca aaaaacatgc cttgcacctc tagtggcgat aagggggcca cccttttatg
    17161 tttgcataaa aaccaggaag gagtatcaga ttagctatcc ttgtgctctt tccaccaacc
    17221 agcactcgct taaaagattc tgtccaatct aacttgtctt caagataatt atagcaagtt
    17281 ttcaaaaatt tacaagtctt aatccagaaa atctttgata gcatggacca gcttgatagg
    17341 agccttgact acttttactg aggtgatagc tgtgacctta gcagcacgtt taggcaagtg
    17401 aatagcacct tgcactaatt ttagagaact acttttacca gaatgattaa gataaagact
    17461 cagcttttga gcttctttgc tatttctagc aattaacgta tctaaataga acgtgtaaat
    17521 agattttcca aagtgctgtg ctgagatttc ataacgtttt tttgctaata accgtttatc
    17581 cataactggt gtttttagta tggcatcaat aatagcatca gttaaatctg tttcagcgta
    17641 ataaagagtg ccaaacattt tatcagtcac cacatcatct aaataaggat tgccatgagc
    17701 aataatagga gtgccactag ccaaactttc aatataggtc aagccctgag tctcactggt
    17761 cgatgctgag ataaagaaat cacaagcctt atagtacaga gcaaccttat catgaggcac
    17821 catgcctgta aaggtcacgt gtttgtcaac ctctaactgc atcgccaagt gtttcaaatc
    17881 ttgcaaatag gggccatctc ctacaataat aagctttatc ttggcatttt cagccaaaat
    17941 agctggcatc tgattgatga tagcttgaat atttttttca taagaaatcc gtgacaaact
    18001 caataacatg gtttcatcac cagcaatgcc caattctgct tttaagttgg ttacttcttc
    18061 tgctgtgatg tcatcacgaa tatatttttc caaaggaatg cctgttggga taacccgctt
    18121 agggatagta acttcgtaac cttctagaag attgaggaca atcctacttg ggcagataac
    18181 cccatccaaa tccttcaaat agcccctaag aagaggtttg accatacttg gtcgaatgat
    18241 ttttccgttg gcaatataac tcacgtagtc ctcatattgg gtatggtaag tatggacaac
    18301 aggaattcgc aaagctttgc ctatcatttt ccctaacaag cctaagctaa actcagtttg
    18361 cgtatgaata atatcaagat tatagtgttt tgcaattttg tatgacgaaa tgaggccacg
    18421 ataaaccaca cgtctatccg taaatgacac aaaaggaaca cttggcagtc gaataatggt
    18481 cgggtcttca aagcgtttga catctctatc agtagtggtg aaaatataaa cttcgtgacc
    18541 ttctttttct agctcttctt ttaacgtacg aatactagta gcgactcctg aaacttgtgg
    18601 aaaataggta tctgtgaata gacctacacg catcttataa ctccattact tttttataga
    18661 catctactaa ggcatggcca accgtttcta ggcgacgact ctgggcaacc ttgtaaccag
    18721 cttcaacttt gttgctggca ccagaaaaga cttttttcag agcttctata aaacctggta
    18781 tatcggttgc taattccgca ctactttgat caacccatcc gtagtaaact ggtatatcac
    18841 gtaaaacaag gtgctgacga ctggccaagg cttctaagac aacaatgcct tctgtttctt
    18901 cacgacttgg aaagaaaaag gcatctgcac cagtcatggc accttcataa acatcccctt
    18961 taatgtatcc tgggaaaata agatttttcg ggtggttacc attgaccatt tggcgaactt
    19021 gagcaggaat gacccatttg ttggtctcgc caaaccagat aaaacgaaca tctggcatag
    19081 cttgggcaac tttgacaaag tcatcaattc ctttcctcag aaaaaataat cctgctccca
    19141 taaccacttt ttcaccctct ttaatgtcaa agtagcgacg aaaagctgct tcctttttag
    19201 aatctgctcc gtactgctcc aaatcaatcc catttgacac tgcaaaaata ggtgacttca
    19261 ctccataact ctcaatcaaa gacttagaat atagggtagg ggtaatgata gcatctgcct
    19321 tattgtaaaa gtgacaaagg tattttttaa accaaggaga taatagattt gaaaaaataa
    19381 aagaatttct aaaatcttct tctgtagaat gcccatgcat gataaccttc ttaccagctt
    19441 tttgtgcttt tatcatcagc agccaacttt ttaaaccata ggtattgaga tgaaccaaat
    19501 cataagtttc tcttggatta gtcgtaaaat gttgaccaac aagtgacaag gctttagcct
    19561 gatgcttaat cgctcgacca attcctgatt ttcttagata attttctgct tctaaataca
    19621 ataagacttt cataagttct attatagctc attttatagt atttttcact aagacatgca
    19681 aaaatcagcc ctaaggctga ttttagtatt gaagaaacag tctattgtga tactttttgt
    19741 tagatggtgc tcataagtca cagttactta gttgtcccac gttttttaat accatgattc
    19801 aaaagaattt ctttttcttc caactcttct ttgttcatga ttttagtcaa catccgcatg
    19861 ctaacagcac ctaagtcata aacaggttga ctgatagaac tcaaattggg acgagtgtat
    19921 tgaaccactg gtgaatcgtt gctggtgatg atttcaaagt cctctggtac gcgtttacct
    19981 gcttcaaaca agccgtttaa aaggcctgct gccaattcat cttcagccac ataagccgct
    20041 gttgctcctg agttaatgac gcgctgcgcc aattcaaatc cttctttata agaatagttc
    20101 gcttcaaaaa caagaccttc tttgaagtca agcttattgt gcttcaaccc ttctttgtaa
    20161 cctgctaagc gcactttacc attgatatca tcaatgagtg gtcctgacac aaaagcgata
    20221 cacttatgat tttcagctaa aatatcaaca acgtttgaca ccgcagctct atagtcaatg
    20281 ttgacgcttg gtaattgatg atcaagatct acagttcctg ccaaaacaac tggagtacga
    20341 gagcgtgaaa actcggcccg gattttttct gtcaagtgat gccccataaa aataatacca
    20401 tccacttgct tggcaaaaag ggtattgaca acattaacct ccttatcatc atcttcatca
    20461 ctggaggcaa gcacaatatt atatttgtac atagcggcga tatcgtcaat acccttagct
    20521 aagatagaaa aataactatt tgcaatattt ggaatcacaa cgccaacggt tgttgtcttt
    20581 ttgctggcga gaccacgcgc gacagcattg ggacgatagt caaggcgatc aataacttct
    20641 aaaactttct tacgtgtatt ttccttaaca tttttattgc cattaacaac acgactaacg
    20701 gttgccattg aaacaccagc ttcacgggca acatcataaa ttgtaatggt atcatctgta
    20761 ttcatttttt atcttccttt ctatttgaaa atgacgcttt caaaaagtat tttagcatga
    20821 tttgtaacca ctttcaagtg ttataggtta atttttgaaa aagttttcaa aaattaactc
    20881 gaagcttgat tttttaagct ttttttgaaa aaatagtaag tagaaagaag gttagcctat
    20941 gacaaaatta gaccaaatac gtttgtatct tgaccaaaag ggagctgaat tggcgatttt
    21001 ctcagatcct gttacgatta actatttaac tggattcttc tgtgacccac atgaaagaca
    21061 actgttcttg tttgtctacc atgatttagc tcctgtgctt tttgtacctg cccttgaggt
    21121 ggccagagct agccaggcca tatcatttcc tgtgtttggt tatgttgatt ctgaaaatcc
    21181 ttgggaaaag atcaaggccg ttttaccaaa tacagcagcc aaaaccattt atgttgaatt
    21241 tgaccacctt aacgtcaata agttccatgg cttgcagact attttttcag gacaatttaa
    21301 caatctaacc ccttatgtcc aaggcatgcg cctcgtcaaa tcagctgatg aaatcaataa
    21361 aatgatgatt gccgggcagt tcgctgataa ggctgttcaa gttggttttg acaatatttc
    21421 cttagatgct accgaaacag acgtcatcgc tcaaattgag tttgaaatga aaaaacaagg
    21481 cattcataaa atgagttttg acactatggt cttaactgga aataatgcag ccaatcccca
    21541 tggcattcct ggaacaaata acattgaaaa caatgccctt cttctttttg acttaggtgt
    21601 cgagacctta ggctacacca gtgatatgac cagaactgtg gctgttggtc agccagacca
    21661 atttaaaatc gatatctaca atctctgcct agaggcacaa ctagcggcta ttgactttat
    21721 caaaccaggt gtaacagctg ctcaagtaga tgctgctgct cgtcaagtga ttgaaaaggc
    21781 tggatatggt gagtacttca accaccgctt gggacatggt attggcatgg atgtccacga
    21841 atttccctcc atcatggctg gcaatgactt ggtccttgaa gaaggcatgt gcttctctgt
    21901 tgaacctggt atttacattc caggaaaggt cggtgtgcgc atcgaagact gtggccatgt
    21961 cactaaaaat ggctttgaag tctttaccca tactccaaaa gagttgcttt attttgaagg
    22021 ctaagcccct atcatctggt taaacaaacc ctcgcgaaag gaaggtattc gtatggatca
    22081 aaccattcat caccaaatac agcaagcact acactttaga acagccgttc gtgtttataa
    22141 ggaagaaaag atttctgatg aggatttagc ccttatcctt gatgctgctt ggttaagtcc
    22201 ttcttctgtt ggcttagaag gctggcgctt tgtcgtttta gacaacaagc ctattaaaga
    22261 agaaatcaag ccctttgcct ggggggccca gtatcaactg gagacagcta gtcactttat
    22321 tcttttaata gccgaaaaac atgcaagata cgatagccct gctatcaaaa atagcctttt
    22381 acggcgtggt atcaaagaag gtgatggtct caacagccgc ctaaaactct atgaatcttt
    22441 ccaaaaagag gacatggata tggcagataa tcctcgggca ctctttgatt ggacagctaa
    22501 acaaacctat atcgctcttg gtaatatgat gatgacagct gctcttttgg gcattgatac
    22561 ttgccctatt gaaggctttc attatgataa ggtcaatcat atcctagcta agcataatgt
    22621 gattgattta gaaaaagaag gcattgctag catgttatcc ctaggctatc gcctgcgaga
    22681 tcccaaacat gctcaagttc gtaagcctaa agaagaagtg atttcagtcg ttaaataagg
    22741 agattaaaat gaaagcatta catacctgta tccgcgtcaa agaccttgac cagtcggttg
    22801 ctttttacac aagcgccttc ccttttaagg aaaactatcg taaagatttt ccagatagtc
    22861 aatttactct tgtttatctg gctttagaag gagaaagtta tgaattagaa ttaacctaca
    22921 attatggtca tggcgactac gacttaggaa atggctatgg tcatattgcc cttggctctg
    22981 aacattttga agcagaccat aaaaaacatc gacaagcagg ttttccagtt accgatatca
    23041 aagaactggc tgacaaatct gcccgctatt atttcattca ggatccagat ggctacaaaa
    23101 ttgaagtcat tgatttaaac aactaatact attaacaaca gaagctttaa agcttctgtt
    23161 gttttatttt ttaacctgac gattgggaca atctcattat taacagagtt ttgaaatatc
    23221 tcagcttttg tgttacaatt gccctaaaat tccatttgaa agaaaagtct atgacactac
    23281 tatctatcat tgtcccctgc tttaatgaag aagcaaacat tctcccttat tttgaagaaa
    23341 tgcatcaact agaaacgtcc atgaccaacc aacttgcttt tgaatacatt tttattgatg
    23401 atggttctaa ggataacacc ttgggtattt tgcgtgagct agctgctcgc tttccaaatg
    23461 tgcattatct gtctttttca cgccattttg gcaaggaagc tggactctta gctggactaa
    23521 aagaagccaa aggcaattac attactgtga tggatgtgga tctgcaagat ccacccgaac
    23581 ttttgccagt tatgtacgct aagctaaaag agggttatga catcgttggc accaggcgcc
    23641 aaaatagaca aggagaacca ctaattcgtt caatgtgttc gaatctattt tatggactga
    23701 tcaaacactt atcagatact gaaatggtca atggagttcg ggattatcgg ttaatgacca
    23761 gacaagtcgt tgatagcatt cttgagttgg gagaggttaa tcgtttttca aaagggatct
    23821 tttcgtgggt tggttatcgt atcacctatc ttagttttga aaatcaaaaa cgcaaatatg
    23881 gaaaaagtcg ttggcatttt tgggagttgc tccgatattc tttagatggc tttatcaact
    23941 tttcagaaat gccgttaacc attgctacct ggacagggac tttcagcttt ttgatttcta
    24001 ttttcgccat cttatttatc attatcagaa agatactctt tggtgatcct gtctctggtt
    24061 gggccagcac tgtctctatt attttattca tgggaggcat tcagctcttt tgcatgggta
    24121 ttattggaaa atatatttca aaaatattcc ttgaaactaa aaaaaggcct ctctatatta
    24181 tcaaagaaaa acattaaatt aaaacatttt tctcatcaat cgtttcctac tccttctgtg
    24241 cgcgaagctt tctttttaag cccgttatac caaatgataa tgacctttcc aaaatagtat
    24301 cactttcctt aacactttaa gtagaaaagg aactattagg ttgagccttt ttgcaaatcc
    24361 tagcgactaa tgttataatc gtcatactat tgaaaaagga gttctttatg gagtggatta
    24421 aattaatcgg tattgtgatt atcgtattag ggtttatttt aaaatttgat gctatcgcta
    24481 ccgtagttgt tgcaggactt gttaccgcgc tggtttcagg aatttcgttt attgattttt
    24541 tagatatttt aggaaaagaa tttaccaatc aacgtttgct aaccattttc ttcatcactt
    24601 taccgcttat cggcttgtca gaaacttatg ggctgaaaca ccgagctact cagttgattc
    24661 aacgtgttca agcccttacc gtcggccgtt tattgactct ctaccttatt attcgagaat
    24721 tagcaggtct cttttcgatt cgactagggg gacatccaca gttcgttcgc cccttgattc
    24781 aacctatggg agaagctgct gctaaagcca acattggaga agagttgacc gatgcagaaa
    24841 aagatgacat caaagctatg gcagctgcca atgaaaactt tggcaatttc tttgcccaaa
    24901 atacctttgt tggcgcagga ggtgtcctac ttatcgctgg aactttagaa cagcttggct
    24961 atgatggtaa ccaagctaaa attgcatttt cttctatttt aattgctatt atctctatta
    25021 ttatagttgc tatttacaat tacctctttg agaaaaaaat ggaacgtcaa catcagaaag
    25081 gagataacta atggctaaca ttgcaaacct tgttttagaa acgatatata tccttatcgg
    25141 cttgcaactg tttcacacag cctactgcac ctttaaagat aaaaccaacc ctgtctattt
    25201 tgggacagct ctgttctggg gattgctggg tgttaccttc gttggcggtg cctttttgcc
    25261 taataaagtt atcggtttta tcgtgattgt cctagctctt ttaaccttgt ttaagcaagt
    25321 tcgtattgga acattaccag cctttaatga acaaaaagct gaagaaagtg ctcaccgcat
    25381 tggtaactgg attttcttac cagttatgtt aatggctatg atttctctac ttcttgcctt
    25441 gatccttcct gatttcagta aaagtgccat tgggattgct ggtatcttag ccactattgc
    25501 tattttaata atcactaagc aaaaaccatc tgctctttta gctgaaaata atcggatgaa
    25561 ccaacaagtg tccacaagcg gtattttgcc acagctctta ggagctttag gagctatttt
    25621 tgcagctgct ggtgttggtg atgtgattgc ttctctcatt cgggagattg tacctgctga
    25681 tagccgcttc tttggtgttc tcgcttatgt gcttggcatg gttattttta ctatgattat
    25741 gggaaacgct tttgctgcct ttaccgttat tacaaccggt atcggtgttc cttttgtttt
    25801 tgctttagga gctgacccta ttattgccgg tgctttagct atgactgctg gtttttgtgg
    25861 taccttatta acaccaatgg cagcaaactt taatgccttg cctgttgctt tgatggagat
    25921 caaagatcga aatgcagtta tcaaaaagca agctcctatc gcccttgttt taattattag
    25981 tcacattgcc ttaatgtatt tacttgctta ttattaactt ttttgctaag ttagtagcgt
    26041 cagttattca ttgaaaggac attattatga aaattcttgt aacaggcttt gatccctttg
    26101 gcggcgaagc tattaatcct gcccttgaag ctatcaagaa attgccagca accattcatg
    26161 gagcagaaat caaatgtatt gaagttccaa cggtttttca aaaatctgcc gatgtgctcc
    26221 agcagcatat cgaaagcttt caacctgatg cagtcctttg tattgggcaa gctggtggcc
    26281 ggactggact aacgccagaa cgcgttgcca ttaatcaaga cgatgctcgc attcctgata
    26341 acgaagggaa tcagcctatt gatacaccta ttcgtgcaga tggtaaagca gcttattttt
    26401 caaccttgcc aatcaaagcg atggttgctg ccattcatca ggctgggctt cctgcttctg
    26461 tttctaatac agctggtacc tttgtttgca atcatttgat gtatcaagcc ctttacttag
    26521 tggataaata ttgtccaaat gccaaagctg ggtttatgca tattcccttt atgatggaac
    26581 aggttgttga taaacctaat acagctgcca tgaacctcga tgatattaca agaggaattg
    26641 aggctgctat ttttgccatt gtcgatttca aagatcgttc cgatttaaaa cgtgtaggag
    26701 gcgctactca ctgaccgtga cgctactaaa cctattttaa aaaaacagag atatgaacta
    26761 actctgtttt ttttgtgcta aaaatgaaag acctagggaa acttttcatc ggtctttctc
    26821 aattgtcatc ttaatctaat actacttcta acatcagcgg gtatagtttg ccagtaatta
    26881 agaaacgttg ttgatctaaa tgagcaatcc cattcaaaac attaaggtca gggtaatggg
    26941 acttatcaag atttaaggct tttaacaaag gactaatatc ataggtggct accacctttc
    27001 cagaatcagg ttggagtttg acaatagtat tggtttgcca aatattggca tagagataac
    27061 catctacata ctctaattcg ttaagcattg agatggggac actttctata gcaactagta
    27121 cggtatccaa taaagcaaaa tctttggggt ctcgcttttg caaaaaagca tttccactag
    27181 tcatccaaag acactggttt tccttatcat aggctaatcc ccagccatct ccttcaaatg
    27241 gataggcccc taaacaattg caggtagcct tatcaaactt gtaggctaca ccctctttat
    27301 aggttagtaa ccaaaaataa tcctcaacaa ctgttagccc ttctgcaaaa actgtatctg
    27361 gaaaagctat tttttctgaa aagatctctt gagtaaggtc atacacccct acttttgaga
    27421 aaccataacg accggcacta agtaatatat gattgttgtt gagttgttct aatccctgcg
    27481 tgtataagtt gctatcataa gaataagttc gtaacaaacg aacttgtcct ttagttatca
    27541 taatactcat tcaccttacc ttgcattgta gtgtttcatt tgttttttaa tatctctttc
    27601 ctgatcacgg cgtttaatgg tttcgcgttt gtcatactcg tgtttccctt tagctagacc
    27661 aatcagaact ttagcaaagc catccttgag gtaaactttg agaggaacca aagtcatacc
    27721 agatccttta agttcatttg ctagatgcgt aatctcacgt tttttgagca acagtttgcg
    27781 ggtccgttca ggatcagcgt tccaaatatt accttgttca aaaggtgcaa tatgcacgtt
    27841 gaccaaccac gcttccccat tcttaatttg ggcaaagcca tctttcagtt gaatacgagc
    27901 tgcacgaaca cttttaatct cagttcccgt taagacaatc cctgcttcaa cggtttcaac
    27961 aatgtgataa tcatgtctag cctttttatt ttgcgccagt atatgacctt caccttttgc
    28021 catagttaac tccttttacg actcttcttt tttgcttgtt ctttgtaaaa tggtttttta
    28081 gacccttttt tagttttacc cttggttttt gccttggcta cttctttagg ttcttttttc
    28141 tttgtccctt tagatgattt tgaggattta cggcgatctc gtcgactagc tttatccgac
    28201 atcttgattt tttcaaccac atcaaagtcg ctaggtaaat actcaaagtc aatgtcacca
    28261 gtttccttgt cagccttaac caatttcaca cgaataggtt gaccaacttt aaagacttta
    28321 cctgattttt caccttgaag gcttagcgtt ctttcattaa agtgatagta ttccggtaag
    28381 ctagtgatat gaaccaaacc ttcaatcgta tttggcaatt ctacaaaaaa accaaattta
    28441 acgacactgg ccacgatacc gtcaaattct tcacccacat actctgccat atactcagct
    28501 tttttcatgg cttcaaccac gcgttcagca tcaatagcac gacgctctaa ctgtgaagaa
    28561 gatgttgcta attctggaat gatctgagta aaatggtcgc gcttttcttg actaggttga
    28621 ctgtactcac gaaccatgcg gtgaaccaac aagtcagggt aacgacgaat cggactggta
    28681 aagtgagtgt aatattctgc ggccaaaccg taatgcccat gattatgttc agaatatctt
    28741 gcctgttgca tagagcgtaa gagcatcatg ttaagcactt ctgctcctgg ttgaccttcg
    28801 acttttgcca taaaggcttg caaggcttcc tgagaaatct tgttggctgt tccttgaatg
    28861 tgaataccaa aggtgctagc gtaatcaata aattgttgaa gtttttcggc ctttggttct
    28921 tcgtgaatac ggtagataaa cggtaactta gcttttgcaa aatgttctgc cacacactca
    28981 ttggctgcta gcataaagga ctcaatcatc cgctctgcaa ttcccctttg acgaagcacc
    29041 acatcaaccg gcattccttt gtcattaaca ataactttag cttcttgcgt atcaaaattc
    29101 aaggcgcctc gttttgaccg catagcttcc aaaatcctat gcaaagccac catgagggtc
    29161 acatcatcag caatgctagc gaattcttgg agcgcttctt catctcctgc aatcatgtca
    29221 ttgaccgtac tatacgtcat tcggtaagtt gtcttaatca ctgattgaca gatttgataa
    29281 ttgaccacat gcccttgaga attaatttcc atcaaggctg actgggtcaa acgatcaacg
    29341 ttagggttca aagagcagat gccatttgac aaacgctctg gtaacatcgg aaccacacga
    29401 tcggtgacat agacagaagt gccacgggcg atagcttcct tatcaagggc agatccttct
    29461 gtcacataat atgagacatc cgcaatatgt accccaagtt catagttacc gttatccaat
    29521 agcttaatat ggatagcatc atctaagtct ttggcgtccg caccatcaat agtaatggtg
    29581 gtttcttgac gcaaatccac acggcctatt aaatctttag cagttggtgc ttctgaaata
    29641 gcattggcct cagctaaaac ttctgctgga aattctgaca cgatatccat cgattcaagg
    29701 acttctagga catcaattcc aacgtctcct tgatggccta cgatatcacg cacactggct
    29761 acaaaataat cgtgcccgcg tgtcgggtat ttatcaatat caaccttgat gatttcagtg
    29821 cctttaagca caacaggttc ttttttgata tagatttttt gttgaatctt ttggtttttg
    29881 gacctgatat aaccagcata ttttggtttg tcatcatcta aaataaaggt tccaacagct
    29941 gtttttaaag aacggtcaac aatagcaacg accttagctt cagccgctgt gcctttgaga
    30001 cggtctgctg gctttttaac cactacttcg acagtatcac catcaatagc atacccaaca
    30061 tcattacgac cgataaacat gtcatcttca ttctcatcca catgtaagaa accaaagcct
    30121 gctttattgg cacgaaaaac accctgaaca gtaggttctt ttttcttttc acgctctttt
    30181 ctaagagcca gactgccatc atctgaaaac cgtaacaggc tctgactttc cattttggaa
    30241 atggttttaa taaggctggg aaaatgctta gctccagcca tctccaaggc cgcagccaag
    30301 tcgttcacat tggacttgcc atgctccttg aggtaagtca taattctttc tttcatcata
    30361 cctaatttct atttctgttt tgtttgttgg aggatatcaa tccgtttcgc tgataaaaaa
    30421 atgagctaga ctaactctgt caagctcact ccttatttac ttgataaaat ggcaagcaca
    30481 agtgcaatcg ctagccaaaa aaatactaga actgctgtga atcgttgcat gaatgcttca
    30541 aagccacgag cttttgtacg ttcaaacaag gcttctgaac cgctgctatc aaaaacattg
    30601 ctacttgggt ttttttgagg ttgcataaaa atagcaatca caagcaatac cgacagtact
    30661 agcaatatcg taagtagtaa gttgtacatg ttatttcctc cactattgtc atgaccattc
    30721 taacacaatt cttatcaagt ttcaacatgc agggtcactt ttctctcctt ggggcagtat
    30781 ttgggaactt ttattttttc cggatgactg cttttatttt tactggttag ataattgtta
    30841 ctgccacact cactacattc taaattaatt tttactctca cacaatatcc ttaactttaa
    30901 tatattttcg aaatataatc aaactccata ttaaattaac aaacacaatc aaactagtca
    30961 cataaaaaac agaacggtag cctaacacta cagccacatt tgatccgata aaagggccaa
    31021 tgacttgacc aaggttacta aacatttgat tataggcaaa tactctagaa ataccttctt
    31081 ttggtgttaa cttagttagt aaagagttaa tacttggcat caaagcgccc acaccaaacc
    31141 cataagcaaa gcgtagtact cctaattgaa aactggtctg cgctaaggca ctgctaaaat
    31201 acatgataaa gctataacac aaagcggcta acaataaccg atgattgcca aaacgatctc
    31261 ctaattttcc taagtaagaa ctgctaaata agctagaaaa ccccatggcc gaaactacca
    31321 ggcctgaagt aaacattaag ttgtgagttt gaccaagatg acgaatatac aatgataaaa
    31381 tgggggccac tgattgagct gaaatttgaa taatcatact agtcacaaac aaaccaagca
    31441 taatctgggg actttttacc tgttttaaaa tgacttttgt aggtatcatc tcaaagcgcc
    31501 taatcggctt aaactcttct ttaacataaa cagctgtcat taaagaacat aaaaataata
    31561 tcacaccaac taataagaaa acttgtcgaa ttcctaatag ctctgccaaa atccccccta
    31621 gtaaaggacc aattaaagcg cctgctgtca ctcctgttgc taatgtgcct aaagcataac
    31681 ctgactcctc ttttggggct tggctggcaa tcaaggcagt ggcatttggc acatagcccg
    31741 caaagacacc tgttaataat ctcaaaatga gtaaccaaaa cacattagga ataatagcta
    31801 aaccacccat ggtaaaggtc atgacaaaac ttgctcgcag catcataggt tttcggccat
    31861 aacgatcagc caacttcccc caaacaggag caaataaagc tgaagcaagt gccgtcacag
    31921 caaccgccag accagcatag tattctacta attctttcgg agtacctaaa ttttccacat
    31981 acagagccat aaaaggcata acaagtgaaa agcttgcccc agtaaagaag ttaccaagcc
    32041 aagccacttt tagattttgc cgccagttca ctgtcgttac cgctttatca ctaatagatc
    32101 atcatcttct ttccatcggg cctttgatta ttataacaca tttctgccaa aaacagtgtc
    32161 ccataatagt gctaagtctc atctgatagc cttcatcgtt tacgtaagct acccagtaaa
    32221 cctctgacca gttctcgccc aattgttcta accgtcgtac taataaacgc atctgttgct
    32281 ttttcgacga ctgtttttcg tggacgccca acagaacggc gactagcttt tttggtagct
    32341 gcttgcgcct tgatggcttc tttagcttct ttttcgcgtt ttgctgtcgc tatcgcttct
    32401 tgggccagtc ggttgtcttc taaaacctta gcagctaatt tttcataagc agattcacgg
    32461 tcaatggact gactatattt actggcaaaa ggtgaagact gcacaagctg ctgactttct
    32521 atctctgaca aaacagcaaa gctactctta ggcggcatga tataggcacg ttcaacgatg
    32581 gatggttgtc ccttatcatt caaaacagaa atcaaggcct caccgacttc tagttctgta
    32641 atcacccttg ccacatctaa gtcaggattt tgtcggaaag tatccgcagc gacacgaacc
    32701 gccttttgtt ctttcggtgt ataagcgcgc aaggcatgct gaatacgatt tccaagctga
    32761 gccaaaaccg tttctggcaa atcaagcggg ttttgagtca caaagaaaat cccaactccc
    32821 tttgaacgaa taagtcgcac aatctgttca actttttcca aaaagacctt aggagcgtcc
    32881 ttgaacaata ggtgagcttc atcaaaaaag aaaaccatct taggtttgtc caaatcccca
    32941 acttctggca ataattcgta caactcagac aagagccaaa gtaagaaggt tgtgtaaagg
    33001 gtaggagatt gaaaaagctt agtcgctgac aaaatgttaa tggctccata acccgaagca
    33061 acatctaatt gcatcaaatc agccacatct aaagcaggct ctccaaaaaa ttggtgggcc
    33121 ccttcttgtt ctaatgtcaa gagacttctt tggatagtcc cgatagattg cttggcgata
    33181 ttaccgtaat ggctggagta gtctgaggca tggtcaccaa cttcttttaa gatagcctgc
    33241 aagtctttca aatcaatcaa aagccaacct ttctcgtcgg cgattttaaa gacgatgttt
    33301 aagacacctg tctgggtatc attaagattc aataaacgcg acaacatcat cggtcccatt
    33361 tcagaaattg ttgtccgtaa gggttgaccg ttttgaccaa aaacatccca cattcttaca
    33421 ggaaaggcct gaggctggta atcacttacc ccgattgtgg ctaaacgcgc agccaattta
    33481 tcagtcacct ctcctgcttt agtaagatta gacaggtctc ctttgatatc tgccaaaaag
    33541 actggcacac cagctaaact taattgctct gctagcactt ttaaggtcac agttttacct
    33601 gttccagtcg ctccagcaat caaaccgtga cgatttagca tccctaattg taaggctatc
    33661 ggttgactgc cttttgcaat tgtcattgta gtcataggct tcctcttttt tgatgatcat
    33721 acttgtcgat atgtgtttac ccataagtta aagtgtttag aagggtctca ctcttaatac
    33781 tattataaca tataaccctt gatgcgctaa tttaaaaggg accagctagt tttagacaga
    33841 aatagcttga ttttgacagc tatgatacga atctctcagc ttagctacct cggattggct
    33901 aatgacaaca acgcagattg tacttgcttt attaaggctg caatatcccc actattatca
    33961 atcactagac tggcataggg ctttttttcc tctattggta actgactggc tattcgctgc
    34021 cttgcttttc ctttgtctaa acgatttcta gccattaagc gctgcaattg tgtctgagca
    34081 tctacataaa ccagccaaat cgcatcaaac cagtcttggt agcccaattc cattaaaatg
    34141 ggaatatcca tgaaaaaaat ggcttggctc tgtgctaagt ggtcacgctt agccgccaat
    34201 tcttccttaa tgatttgatt ttgaatggct gaggatgttg ccatattatc tgggtttgaa
    34261 aaaagcattt ctgacagctt tgtgcgatct aactcaccat ctgcttttaa aatctgattc
    34321 ccaaaagctt ctctaagagc ctcgtaaaga cgaccgcctt tttcttgcaa gtcatggacc
    34381 acttgatccg catcaatcac ctgataacca gcttttctaa tgaccttaac cacagtcgat
    34441 tttcctgatg caatgcctcc tgtgatgcca ataatcatgg tctttttttc tggcattttg
    34501 gacaaatgtg cgtccccctt cctccaactt ttaatttgac aatagcctgt ccacaacgag
    34561 gacacggttt cccagtttga ccataaacct gcaaatagtc ctgcatggtg ccatcagctc
    34621 ccaaagcatt acggtaagta cggacggtcg aaccgccctt ttcaattcct aaagctagga
    34681 ttcgtatggt ttcatcatgc agccgtttta tctccgcctt attcaaccta gaagacgcag
    34741 ttcctggatg aaccttagca gcccaaagta cctcgtccac ataaatattc cctaaaccag
    34801 caaccaaggt ctggtctaac agatgtggtt ttatgggttt ttgggaggat agcagagcag
    34861 cctcaaaggt ttttaactta aaggtctctt tctttggttc agggcctagt tttcgtttgg
    34921 caaaaaacgc tgataattga gattttgcta tcaaatcaaa ggttccaaat ttacgcacat
    34981 cttggtaaac caaggtcgaa ccatttttca attcaaaaaa gacatgaaag tgtttgttat
    35041 cagggacctc atctggaaaa agcaaatatt tgccttccat ccgcaaatgt gataccaaca
    35101 ctaattgccc caaatcaatt aataaatatt ttccccgacg gccaactgac tgaatgattt
    35161 gaccaggtag ggtcagggca aacgtctcta agtcggtttt aaccatcttt ggcactttaa
    35221 gcgtaacagc cacaatttct tggccaagca ctaaagtctc aagccctcgt cggaccgttt
    35281 caacttctgg taattctggc ataatccgtt atttaaagga cagctagacc ctttgctcct
    35341 acaaagaata aactcagtat aaaaaagtca ctcaatagac tcaggtctag gctattgact
    35401 gcgtttcaaa aaacacagct gtccttctcc tttcttattt cttattttac gaacacatct
    35461 gatagaaagc aactcataaa tctgcctcag accagtcctc atccccctta cctcccagca
    35521 ctatttggcg gtaatgttcc ttataactat aatacaatga gtcctctcca agatacttaa
    35581 aaatctgcaa ggcctgccgc atgtcctctt ctccagacat ttcttcctgc tttaatttat
    35641 aatacccatg cacatataag aaaacagtct tttcataaaa attgagctcg tctcgcagca
    35701 aaagatcaat tttgttaatc aaatagcgac tatgttcaaa gcaagaatgg tcaatactga
    35761 taatgagaca attaatggat aactgagtca ctaacatttt attggtttta ttttgttccg
    35821 aataagcaaa agatgctacc atttccttag tcaataaaaa tagggtattg taattcatga
    35881 agcgcgagca attccccaga agaatgattt cataatatcc ccactgttcc actttaaata
    35941 aataatctgt gaggcgagtc aattcttctt gactaggtgc aattgaggag tcaatagaaa
    36001 aaagaatagc tttaatcatc gttcgttcat aatctttatg ggcataatcc ttcaacagtt
    36061 tggtcaattt aaccacgttt ttctcagcat aacattttct cgcctgactt aagagcgtga
    36121 aaaaatgcgt atgcgtcttg gaatgagccg aaacaaattc gtcaatcgta atattcagtt
    36181 tatccaaaag attaagcaag cgactgcagg tgatttctga ttcccctcgt tcaaatcttg
    36241 aaatttgtga cttggacagg tattcatctg ctaaaaaaga aatgctaacc tgcttaccct
    36301 tacgtaatcg ccttaaggtt tttccgagtt ctttttccat ttttcccact ttcacaacaa
    36361 aaaattaaaa tctgctaaat ccattatata atacaagtac aaaatagaaa atcagaaagg
    36421 agacagtcat gaaaaaagtt aataaagctt tgctttttac attgatcatg gatatcctga
    36481 ttatcgttgg tggataacct ttcgcttaaa aacaatccac ttttgggaaa cccttatatt
    36541 tagtttgtaa caggaaaact aataaatatt aaaaagagtg ttagcgacct agcggcagct
    36601 ttactcttaa gtgcgtttgc tgccattgac agcaaggtca aagctaatga aacagcagga
    36661 gcataccgtt atgatgtgtc tgaacaaaac acttactatc aaactatcag ctccattaac
    36721 cagcaacgac ctggtgccac tctcgacgaa tgaggtgatt attatggaac ccttacttca
    36781 tttacttccc aaaatcgtac cagtacttat acacttggta gacaaagcca acgtatcatg
    36841 gttaacagtg atattacagt tacctatact cttaaagaca atttggtagc ccttcataag
    36901 gaatataaaa cctttgtcaa aaaaatatta cagataaacc gatcaccagt gactacgaca
    36961 aggctaaggt tatttataac catttactca acgaatacac ttacgccaca aaataactta
    37021 gcaccaatca caaaaccgta agcagcatca acaatcatac tcctaaagct ctttatcgtg
    37081 gcaagtgtgg tgtttgtcaa gcttatgccg tcatgtttaa agatatggca gaagctgcag
    37141 gcttatcagc ttggtatgtg ataaaaaagg attgaatgag caccatgctt gaaacatcgt
    37201 taccattcat ggtgttaatt cctacgttga tgctacctgg aatgattcag catacaccac
    37261 aaggtatttc cttgctggaa aagacgtgat gaatactgag taccgtttag atagccagta
    37321 caaccacttg gctaatacta ttttaactac cagctaccaa aaaggctaaa ctaacaacaa
    37381 ctacattttt caactagaaa ggcctaaaca atgaccaaaa aacagtttta tcttatctta
    37441 gctattagca tactgtgttt tattcttgcc gtcagcccta aaccaccaat tcctaaacac
    37501 actattgaaa ggaaaaggac tgcgatgacc ataggccact caaaacaaac ccctcacaaa
    37561 aaagaagccc ttaaacacaa aaaaaacaaa aataaggctc tatttcttcc aagtggggac
    37621 agcgctatca aatctgttat tagcatgaca gttagactcc tgttagatga ccaatattag
    37681 ctctcctaaa atcagtggac actaccattt cggtagtgtt cttttttaag ctaatctcat
    37741 caccacgctt tcttcaagct aaaaaactga tcatgaaaga gcaaccccat caccctaaca
    37801 gcccactttg cccgcacttt cttaaaaagt taggagaaaa cgatatacca attattttct
    37861 gatcccagct ccctatcatt aacaagatgt gtagcacgga cctacataca ggtaagctag
    37921 agtcattctc gctacttcgg acaaaaagag gccggtagtt ccctgacctc ttctttattt
    37981 atcttgtaac actcctcttc ttagcgagac ttactagcac ataattctta agcactctta
    38041 tcatttagac taggaataac accatctgaa tgaagcaaat gatgcgtcag atctgccaga
    38101 gtatcatctc cataagtaat ccctgtttgg tgctgtttct gatgaaaatc agatgactcc
    38161 ttaactttag cagtcacctg ctggtcactt ggatgaaaat taatttctct cttggcttca
    38221 gccgcaactg gctcgaggaa caacttattg acattacggt cttcctccaa atgattaagg
    38281 atagcagcat catcttctaa cgaaagaacc ttcttgggta acaaaaaggg gttatttagc
    38341 cttggctcag taacgacttc cttggctact cgtgatagtt tattattgtc ttcctcaggt
    38401 atcaaacggt taggcaatcg atgcaattct tccggctatg aggcgctcac agctacttca
    38461 gaagaaacct ccaagccctt tcctgtttgc ctgtctaact aaagcaaccg gcaaattatt
    38521 aactaacttt ttaacctgtc ctcagcgctc atttcaacac taggaagagc tgtcgctggt
    38581 tctagccagc taccaacttc tgctggtctt accttaacat cgctggcagg cttctccttc
    38641 tcatcttcat taggtctaaa aggctcaaac cgccctgttg atttttcatc tatgaccttt
    38701 atattgtctt ttactacatg agacagtggt tcgacattta ccatagcttc atgctcgtta
    38761 aggctctggt ctcgtttgaa tttaccagga ataccaggag tcggtgttcc gacagattga
    38821 tcggcttcag taggctcttt tacggagcct ttttcccata acaccgctga tggaatgata
    38881 tccgtagagc catctccgta aataatcgtg gcctgaccac ctcttggatt aatagatacc
    38941 ttaatattgt ctgggaaagc cccactactt cctgtggtta aaagggcacc cgcattttta
    39001 atgagagttg tcattcgttc aataccagca acgccaatat ggttcaagtc atctactggt
    39061 aactgatcaa cagatttcat ggtgtatttc caagcttgcg gttgaacaag aaaaacagtt
    39121 cctttataca ttgtgttatg catatcatca atgactttga aataccttgt atatttggtt
    39181 tgtacattag ggttagcaac gggaacagtg ctttcaggaa ctcctgacat cgtaaaaatc
    39241 cctctagcag aagtttgaaa gccctcataa acatgaccca taccaggaac agtctgagcg
    39301 gtcaaagact taccagaacc atccaaagca atgcgaatat ctttcatcag atacatctga
    39361 ttggtgtagg taaacctaac cgtgttagcc tcacctcgat acaaaaccac ttgggagggc
    39421 tgatccgaag aaaactgaga ggttgtttct tccttatagc caactccaaa acgtctatca
    39481 gacgaatgga ctgaatctgc cgcaactgta ctactcaaac tgactagtaa agtagccgca
    39541 caaccaacta ataaactgct cattttaact ttaacatctc ttaacatcat catgttcctt
    39601 tcattatttt ttatttagga attcgtcata ctatattttc tcatattagt ctaacaaaaa
    39661 acccgtaatt ctatataaat catataaaat gacgagcttt ggcaaagatg agtatagcag
    39721 gatggaaaac agattgtttc tgaaacttca aaggtatctt cttgtcacga actattcttt
    39781 aattgaatcg gttacactaa actagtccca tta
//
LOCUS       DATXQK010000013        39472 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.013, whole genome shotgun sequence.
ACCESSION   DATXQK010000013 DATXQK010000000
VERSION     DATXQK010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 39472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39472
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.013"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            374..808
                     /locus_tag="VC394_000719"
     CDS             374..808
                     /locus_tag="VC394_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994210.1"
                     /translation="MTKKNHGVFWGLLLGSAAASIAYLSLSSSKKDQLLKDSAKKIDD
                     LNAYLQDKSKQVLDAVSEKVQESKDAVEVYGGIAAETVEEKWQDTKDAVEVYGGIATE
                     TVEESLGQVKEKVEGIGEATSQTIQSKMEKLTSDKTDADDEK"
     gene            complement(858..1769)
                     /locus_tag="VC394_000720"
     CDS             complement(858..1769)
                     /locus_tag="VC394_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEP5994211.1"
                     /translation="MKTVRIFYNPNSGKKENQLARQVKDYFCQHGFSEDSVEVITPKD
                     ADQAFQLAKQAAKDKIDLVIPLGGDGTLNKIIGGIYEGGAHCLIGLVPSGTVNNFAKA
                     MHIPLQITEALDTILTGQIMQVDICKANQQYMISSLTLGLLADIAADVTAEEKRRFGP
                     LAFLKDSIRILKRNRSYAISLISHNHRIHLKTKFLLITMTNTIAGFPSFSPGAQADDG
                     YFQVYTMKKVSFFKFLWHINDFKQGEFSKAEEISHFQANTLSLLPQAKKQAILPRTRI
                     DGDKSDYLPIQLDIIPKAISIIVPTED"
     gene            complement(1886..2536)
                     /locus_tag="VC394_000721"
     CDS             complement(1886..2536)
                     /locus_tag="VC394_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEP5994212.1"
                     /translation="MNQTFKQSLPLSFSEEVANSVTHAIGAFAMLILLPISASYAYQT
                     YDLKAAIGISIFVISLFLMFLSSTIYHSMAYGSVHKYILRIIDHSMIYIAIAGSYTPV
                     ALSLVSGWLGYIIIVLQWGITLFGILYKIFAKRINEKFSLMLYIVMGWLVVFILPVII
                     QKTSLAFGLLMLFGGLSYTIGAVFYAKKRPYFHMIWHLFILLASALQFIAITFFML"
     gene            complement(2533..2973)
                     /locus_tag="VC394_000722"
     CDS             complement(2533..2973)
                     /locus_tag="VC394_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEP5994213.1"
                     /translation="MIFPSLPYWKDLPDLDLYLDQVLLYVNQCTDFSEVSDNKSLTAS
                     MINNYVKHGYVTKPIKKKYQKQQLARLIAISLFKTVFPIQDISRVLEELQAQADSESL
                     YNTFVTCWNQKAPIEEDIPPIVQVACQTVKDYHKTIYLLQEVIQ"
     gene            3192..4040
                     /gene="ylqF"
                     /locus_tag="VC394_000723"
     CDS             3192..4040
                     /gene="ylqF"
                     /locus_tag="VC394_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEP5994214.1"
                     /translation="MATIQWFPGHMSKARRQVQENVKHVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLMILNKADLADATRTKEWKAYYESQGIKTLAINSKEQSTVKKVTEAAK
                     ELMADKIQRLRERGIQKETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKSNKELEILDTPGILWPKFEDELVGLKLALTGAIKDQLLPMDEVTIFGLNYFKTHY
                     PSRLTKRFKNIPLEEEAPEIIMTLTRQLGFKDDYDRFYTLFVKEVRDGKLGQYTLDQV
                     GDMDVD"
     gene            4030..4821
                     /locus_tag="VC394_000724"
     CDS             4030..4821
                     /locus_tag="VC394_000724"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEP5994215.1"
                     /translation="MSTSIKAIKESLEAVTSLSDPLFQELATDARLGVQKALKSRQKA
                     IQADLAEEERLEAMLSYEKALYKEGYQAIAGIDEVGRGPLAGPVVAACVILPQHCKIK
                     GLNDSKKIPKSKHETIYQAVKEKALAIGIGIIDNQLIDEVNIYEATKLAMLEAIKQLE
                     GQLTQPDYLLIDAMTLDIAISQQSILKGDANSLSIAAASIVAKVTRDQMMANYDRIFP
                     GYGFAKNAGYGTKEHLQGLKAYGITPIHRKSFEPVKSMCCDSTNP"
     gene            4886..5722
                     /gene="dprA"
                     /locus_tag="VC394_000725"
     CDS             4886..5722
                     /gene="dprA"
                     /locus_tag="VC394_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEP5994216.1"
                     /translation="MNHFELYKLKKAGLTNKNILNILDYQEHQEKSLSLRDMAVVSGC
                     KHPSHFIEAYKQLDIQKLKIEFKQFPSISILDKHYPMALKEIYNPPVLLFFQGNLDLL
                     EKPKLAIVGSRRSSDTGVKSVRKILKELGNRFVIVSGLARGIDTSAHLACLKNGGQTI
                     AIIGTGLDRFYPKENRELQTFLGKNHLVLTEYGPGEEALSYHFPERNRIIAGLSRGIL
                     VVEAKNRSGSLITCQIGIEEGRDIFAVPGNILDGKSEGCLQLIKEGATCVTSGMDILS
                     EY"
     gene            5829..7958
                     /gene="topA"
                     /locus_tag="VC394_000726"
     CDS             5829..7958
                     /gene="topA"
                     /locus_tag="VC394_000726"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEP5994217.1"
                     /translation="MGTKTTTKPKTGTKKSTTKKKSTAKKNLVIVESPAKAKTIEKYL
                     GRSYKVVASVGHIRDLKKSSMSIDFDNNYEPQYINIRGKGSLINSLKKEAKAAKKVYL
                     ASDPDREGEAISWHLSHILGLDPQDNNRVVFNEITKDAVKHAFVEPRQIDMDLVDSQQ
                     ARRVLDRIVGYSISPILWKKVKKGLSAGRVQSVALKLIIDRENDIKAFVPKEYWSIDG
                     LFKKGTKKFQATFYGINGKKTKLDNNNDVKEVLAKLTNEDFLVSKVDKKERRRNAPLP
                     YTTSSLQQDAANKINFRTRKTMMVAQQLYEGIHLGENGTQGLITYMRTDSTRISPVAQ
                     NDAAQFIINRFGANYSKHGNRVKNTSGAQDAHEAIRPSSVNHTPDSIAKYLNKDQLKL
                     YTLIWNRFVASQMTAAVFDTVKVNLEQNGVIFVANGSQMKFDGYMAVYNDSDKNKMLP
                     EMAEGETVKKISTSPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTLEVIQRRYY
                     VKLSAKRFEPTELGEIVNKLIVEFFPDIVDVAFTAEMEGKLDQVEIGEEQWQHVIDQF
                     YQPFVKELNKAESEIEKIQIKDEPAGFDCDVCGHPMVIKLGRFGKFYACSNFPECRNT
                     KAITKEIGVTCPVCHKGQVIERKTKKNRIFYGCDQYPDCEFISWDLPIGRACPKSGDY
                     LIEKKVRGGKQVMCSNETCDYKEEKIK"
     gene            complement(8033..8917)
                     /locus_tag="VC394_000727"
                     /pseudo
     CDS             complement(8033..8917)
                     /locus_tag="VC394_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354842.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            8930..9475
                     /locus_tag="VC394_000728"
                     /pseudo
     CDS             8930..9475
                     /locus_tag="VC394_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631865.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
     gene            9490..10482
                     /locus_tag="VC394_000729"
     CDS             9490..10482
                     /locus_tag="VC394_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEP5994218.1"
                     /translation="MKLKLYNVRGEEAVLAKKWADDNGIEISLTESPLTPETVKEAEG
                     FDGIANAQIGPLDDAIYPLLREMGIKQIAQRSAGVDMYNLDLATENDIIITNVPSYSP
                     ESIAEFTVTIALNLIRHVELIRENVKKQNFTWGLPIRGRVLGDMTVAIIGTGRIGLAT
                     AKIFKGFGCKVVGYDIYQSDAAKAVLDYKESVEEAIKDADFVSLHMPPTAENTHLFNA
                     DLFKSFKKGAILMNMARGALIETQDLLDALDAGLLSGAGIDTYEFEGPYIPKNFEGQE
                     ITDSLFKALINHPKVIYTPHAAYYTDEAVKNLVEGALNATVEIIKTGTTTTRVN"
     gene            10587..11252
                     /locus_tag="VC394_000730"
     CDS             10587..11252
                     /locus_tag="VC394_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HEP5994219.1"
                     /translation="MTYIALIGDIIQSKQLTDRSKVQKTLAAYLDDLNKTFAPYIISK
                     LSLTLGDEFQGLFQVDTPIFHLIDLINHHMDIPIRFGVGVGSILTDINPDISIGADGP
                     AYWHAREAIRYIHQKNDYGNTTLAFRTGHHNQDDVLNSLLAAGDAIKANWRASQWEIF
                     DTLLDLGIYEEYFDQQRLGKQLSLSSSALSKRLKSSHVKIYLRTRQSALNCLKQIKEE
                     TDD"
     gene            11245..11961
                     /locus_tag="VC394_000731"
     CDS             11245..11961
                     /locus_tag="VC394_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HEP5994220.1"
                     /translation="MINGVSHYLAQTPTLTLFLICHVLSDYQLQSQQVADLKEKHLTY
                     LGYHLIGVSIPLICLTLIIPQAWLMSLLVMISHALIDWLKPKMANSLKWKREWIFLLD
                     QCLHIAISSFAGLRLAGVTLPNWLPISILMTVLFILLITKPTNIVFKLFFSKYQPDQG
                     EKMDTIIGAGATIGILERIVIGVCMFMGQFASIGLVFTAKSIARYNKISESPAFAEYY
                     LIGSLFSILSVFIAAWICFF"
     gene            12107..13453
                     /gene="trmFO"
                     /locus_tag="VC394_000732"
     CDS             12107..13453
                     /gene="trmFO"
                     /locus_tag="VC394_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEP5994221.1"
                     /translation="MSQSTATYINVIGAGLAGSEAAYQIAKRGIPVKLYEMRGVKATP
                     QHKTTNFAELVCSNSFRGDSLTNAVGLLKEEMRRLDSIIMRNGEANRVPAGGAMAVDR
                     EGYAKSVTAELENHPLIEVIRDEITEIPDDAITVIATGPLTSDALAEKIHALNGGDGF
                     YFYDAAAPIIDKSTIDMSKVYLKSRYDKGEAAYLNCPMTKEEFMAFHDALTNAEEAPL
                     NAFEKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQ
                     LRQDNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLL
                     TETFQSRNNPNLFFAGQMTGVEGYVESAASGLVAGTNAARLFKREEALVFPQTTAIGS
                     LPHYVTHADSKHFQPMNVNFGIIKELEGPRIRDKKERYEAIASRALADLDTCLASL"
     gene            13606..15012
                     /locus_tag="VC394_000733"
     CDS             13606..15012
                     /locus_tag="VC394_000733"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HEP5994222.1"
                     /translation="MIQFGGFSMQQQVAITETVLRDGHQSLMATRLSIEDMLPVLTIL
                     DKIGYYSLECWGGATFDACIRFLNEDPWERLRTLKKGLPNTRLQMLLRGQNLLGYRHY
                     ADDIVDKFISLSAQNGIDVFRIFDALNDPRNIQQALRAVKKTGKEAQLCIAYTTSPVH
                     TLNYYLSLVKELVEMGADSICIKDMAGILTPKAAKELVSGIKAMTNLPLIVHTHATSG
                     ISQMTYLAAVEAGADRIDTALSPFSEGTSQPATESMYLALKEAGYDITLDETLLEQAA
                     NHLRQVRQKYLADGILDPSLLFPDPRTLQYQVPGGMLSNMLSQLKQANAESKLEEVLA
                     EVPRVRKDLGYPPLVTPLSQMVGTQAAMNVILGKPYQMVSKEIKQYLAGDYGKTPAPV
                     NEDLKRSQIGSAPVTTNRPADQLSPEFEVLKAEVADLAQTDEDVLTYALFPSVAKPFL
                     TTKYQTDDVIKVTAFIKA"
     gene            15055..15369
                     /locus_tag="VC394_000734"
     CDS             15055..15369
                     /locus_tag="VC394_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994223.1"
                     /translation="MTYSFIDILQVTLVSLFLVLTCLTGIMGLMMLSGKLLSQEKKTL
                     SQQASSIPQEQEADKLSGEALETLLAKDELARIAVMTSLAYASQEESGKRFEVIAVER
                     KS"
     gene            15369..15719
                     /locus_tag="VC394_000735"
     CDS             15369..15719
                     /locus_tag="VC394_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEP5994224.1"
                     /translation="MKRYEVTVNGQVYEVSLLELADGETVQVSRPAAPATEKEMNANA
                     AGGGLQVKAPMSGTVLSIFATEGKAVKKGEAVLVLEAMKMENEILAPADGLVSKIHVV
                     ANQTVESEQVLISF"
     gene            15729..16859
                     /locus_tag="VC394_000736"
     CDS             15729..16859
                     /locus_tag="VC394_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HEP5994225.1"
                     /translation="MLDVLNQMVQSSGLAHLTVNNLIMICLACFFLYLGIKKEYEPYL
                     MVPIAFGILLVNLPMAGLMDHPANGNPGGLLYYLYKGTSLGIYPPLIFLCLGASTDFG
                     PLIANPKTILLGGAAQVGIFLAFFLAIMLGMTPQEAASVGIIGGADGPTAIYVTTKLA
                     PDLLSTIALAAYSYMALVPIIQPPIIKLLTTKAERQVKMTQARTVSQKEKIIFPIMVT
                     IFVSLLVPSATTLVGCLMLGNLVREIKIVPKIVENLQQVVMFCITIILGLTVGAKANG
                     DLFLSATTLKIIALGLIAFAAGTAGGVLMGKVMYYLSGNKVNPMIGAAGVSAVPMAAR
                     VVQKVGQEEDPSNFLLMHAMGPNVAGVIGSAIASGALLAFFG"
     gene            complement(17060..17944)
                     /gene="citG"
                     /locus_tag="VC394_000737"
     CDS             complement(17060..17944)
                     /gene="citG"
                     /locus_tag="VC394_000737"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HEP5994226.1"
                     /translation="MTKAVLTSISQLALKALLYEVSLSPKPGLVDRFDNGAHDDMSFM
                     TFIDSMIALSPFFQAYIETGFAYAKEEPLLLFNRLRQLGQKAEETMFCATQGINTHKG
                     LNFSMALLLGATGAYLARTPHLMTDLGCFSKEDTLAICRLVKPMTAHLIQTDLGHLNT
                     KKEFTYGEQLFVTYGIKGPRGEASEGFTTLTDHALPYFRQMISQNDPETSQLRLLVYL
                     MSIVEDGNLIHRGGIEAWKGVKADMRLLLQQDLSTTDLRLALSSYNQCLINQHLSPGG
                     AADLLALTFYFAFLEKLL"
     gene            complement(17937..18632)
                     /locus_tag="VC394_000738"
     CDS             complement(17937..18632)
                     /locus_tag="VC394_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEP5994227.1"
                     /translation="MNPIIEAVKQNLDLSRNIPLKIAFYNALKTTIILRQIPVGSRIN
                     EKEFSIALNISRTPIRYALGLLSEEHLVEHIPKKGIIVKGVSIKDACEIFEIRKALET
                     LATVQAMHLMTEEDFKVMHNLLEDCETFIAEDDTNRILDNFNAFNNLIYSYSQMVRLK
                     EIVTELQAYLVYFRKISISSVERRKRALSEHWMIYRGMKNKDHEQITLITHEHLNSSL
                     EFILKEMRPEQND"
     gene            complement(18790..20193)
                     /locus_tag="VC394_000739"
     CDS             complement(18790..20193)
                     /locus_tag="VC394_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HEP5994228.1"
                     /translation="MLLTMLAYAMIIVFMYVVMKKKMTPFTALVMIPLIMTIAVILTG
                     SADFNADAKFVAFVGDGGIAKDLTAIGPMVMYGINNTAKTGIMLLFAILFFSVMLDAG
                     LFDPITEKMIRFAKGDPMKVLIATAVVAAAVSLNGDGTTTTLICCSAFLPIYKKLDMK
                     IMNLGVLIILQNTIMNLLPWGGPTARAMSVLGVGPEILGYLAPGMILSLLYVICWVAP
                     SMGRKERARLGVIDLSEEDMRQLTDITDPDTLFIRRPKNFVFNAILTIGLITWLVAGS
                     FNKSIAMAPLLLFAVGTCIALMVNYPVLKDQSKRIGDNAGDAVQVVILVFAAGIFMGL
                     FQGSGMASALAQSFATIIPKQLAGFWGLVIALVSAPGTFFISNDGFYYGILPVLAEAG
                     AEYGFSNMAMALASLMGQAFHLLSPLVAFIYLLLRLTGLDMGEWQKEAAKYALIIFVI
                     FVVTIIAMGQMPLYIPQ"
     gene            20573..20896
                     /locus_tag="VC394_000740"
     CDS             20573..20896
                     /locus_tag="VC394_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994229.1"
                     /translation="MQFLQKWFKHSKKEKLKESSPLSTEIEPSENWEKIPAYIPADKS
                     DYKKVTLITSAIAAGDRPNSQFKVKRILKRNPEAITVSLIASSIAAGVYPESQFRVTS
                     IYCKR"
     gene            20953..21348
                     /locus_tag="VC394_000741"
     CDS             20953..21348
                     /locus_tag="VC394_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HEP5994230.1"
                     /translation="MLRKFKITIDGKEYLVEMEEIGAPAQAAAPAQPISTPVPVPTEA
                     SPQVEEAQAPQPVAAAGADAIPSPMPGTILKVLVAVGDQVTENQPLLILEAMKMENEI
                     VASSAGTITAIHVGPGQVVNPGDGLITIG"
     gene            21393..22514
                     /locus_tag="VC394_000742"
     CDS             21393..22514
                     /locus_tag="VC394_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019323213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HEP5994231.1"
                     /translation="MDTLIAGITSITIPQIVMMVIGALLMYLGIKKEYEPTLLVPMGL
                     GTILVNFPGSGVLTQVVNGVEQEGVFEALFNFGIGTELFPLLIFIGIGAMIDFGPLLQ
                     NPFMLLFGAAAQFGIFFVVVVAVLAGFDIKEAASIGIIGAADGPTSIFVANQLAKDLL
                     GPITVAAYSYMALVPIIQPFAIKLVTTKKERRIRMTYKAENVSQMTKILFPIIITLVA
                     GFIAPISLPLVGFLMFGNLLRECGVLDRLSQTAQNELVNIISILLGLTISIKMQADLF
                     LNVQTLLIIVFGLLAFIMDSIGGVMFAKFLNLFRKEKINPMIGAAGISAFPMSSRVIQ
                     KMATDEDPQNFILMYAVGANVSGQIASVIAGGLLLAYFS"
     gene            22702..23010
                     /gene="citD"
                     /locus_tag="VC394_000743"
     CDS             22702..23010
                     /gene="citD"
                     /locus_tag="VC394_000743"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HEP5994232.1"
                     /translation="MDIKQTAVAGSLESSDLMITVSPNDEKTITITLDSSVEKQFGNH
                     IRQLIHQTLVNLKVTAAKVEAVDKGALDCTIQARTIAAVHRAAGVDQYDWKEIDSWNV
                     "
     gene            22998..23885
                     /gene="citE"
                     /locus_tag="VC394_000744"
     CDS             22998..23885
                     /gene="citE"
                     /locus_tag="VC394_000744"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HEP5994233.1"
                     /translation="MERLRRTMMFVPGANAAMLRDAPLFGADSIMFDLEDSVSLKEKD
                     TSRALVHFALKTFDYSSVETVVRVNGLDSCGALDIEAVVLAGVNVIRLPKTETAQDIV
                     DVEAVIERVERENSIEVGRTRMMAAIESAEGVLNAREIAKASKRLIGIALGAEDYVTN
                     MKTRRYPDGQELFFARSMILHAARAAGIAAIDTVYSDVNNTEGFQNEVRMIKQLGFDG
                     KSVINPRQIPLVNEIYTPTEKEIDHSKQVIWAIREAESKGSGVISLNGKMVDKPIVER
                     AERVIALATAAGVLSEEDI"
     gene            23888..25420
                     /gene="citF"
                     /locus_tag="VC394_000745"
     CDS             23888..25420
                     /gene="citF"
                     /locus_tag="VC394_000745"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HEP5994234.1"
                     /translation="MVTNKLGRDIPQPYADQYGVFEGELANIKQYDESSRRIKPVKPG
                     DSKLLGSVREAIEKTGLTDGMTISFHHHFREGDFIMNMVLEEIAKMGIKNLSIAPSSI
                     ANVHEPLIDHIKNGVVTNITSSGLRDKVGAAISEGLMENPVVIRSHGGRARAIASGDI
                     HIDVAFLGAPSSDAYGNVNGTKGKATCGSLGYAMIDAKYADQVVILTDNLVPYPNTPI
                     SIPQTDVDYVVTVDAIGDPQGIAKGATRFTKNPKELLIAEYAAKVITNSPYFKEGFSF
                     QTGTGGASLAVTRFMREAMIKENIKASFALGGITNAMVELLEEGLVEKILDVQDFDHP
                     SAVSLGKHADHYEIDANMYASPLSKGAVINQLDTCILSALEVDTNFNVNVMTGSDGVI
                     RGASGGHCDTAFAAKMSLVISPLIRGRIPTFVDEVNTVITPGTSVDVVVTEVGIAINP
                     NRQDLVDHFKSLNVPQFSIEELKEKAYAIVGTPERIQYGDKVVALIEYRDGSLMDVVY
                     NV"
     gene            25413..25991
                     /gene="citX"
                     /locus_tag="VC394_000746"
     CDS             25413..25991
                     /gene="citX"
                     /locus_tag="VC394_000746"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HEP5994235.1"
                     /translation="MCKDTYFSGEAIQLSDMLRAREERALRQLHLLKEYPEGSLLSVT
                     MNIPGPIKTSPKLLEVFDIVIKAIQTALADDKICYQLRLLPTTGYEYYLITSLPSRDL
                     KLKMIALETELPIGRLMDLDVLVLQNDLPHSISRTVLGGSPRQCFICSKEAKVCGRLR
                     KHSVEEMQTAISKLLHSFFNKDNQSSSSDKTG"
     gene            26006..27400
                     /locus_tag="VC394_000747"
     CDS             26006..27400
                     /locus_tag="VC394_000747"
                     /EC_number="4.1.1.112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
                     /protein_id="HEP5994236.1"
                     /translation="MTKIRITETVLRDGQQSQIATRMTTKEMIPILETLDNAGYHALE
                     MWGGATFDSCLRFLNEDPWERLRAIRKAVKKTKLQMLLRGQNLLGYRNYADDVVRSFV
                     QKSIENGIDIVRIFDALNDPRNLQTAVSATKKFGGHAQVAISYTTSPVHTIDYFVELA
                     KAYQAIGADSICIKDMAGVLTPEIGYQLVKCIKENTTIPLEVHTHATSGISEMTYLKV
                     AEAGADIIDTAISSFSGGTSQPATESMAIALTDLGFDTGLDMQEVAKVAEYFNTIRDH
                     YREIGILNPKVKDTEPKTLIYQVPGGMLSNLLSQLTEQGLADKYEEVLAEVPKVRADL
                     GYPPLVTPLSQMVGTQALMNIISGERYKVVPNEIKDYVRGLYGQSPAPLAEGIKEKII
                     GDEAVIACRPADLIEPQMIYLRDEIAQYARSEEDVLSYASFPQQARDFLGRREDPFYD
                     VPVQEVTVQLDIQD"
     gene            complement(27510..28562)
                     /gene="citC"
                     /locus_tag="VC394_000748"
     CDS             complement(27510..28562)
                     /gene="citC"
                     /locus_tag="VC394_000748"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HEP5994237.1"
                     /translation="MPYYTISKVFPSDKTTMASVKNLLHQEGIRLDAHLDYTCAIMNA
                     QNDVIATGSYFGNSLRCLCVSSAYQGGGFLNRIVSHLIDEEYALGNYHLFVYTKTSSA
                     AFFKDLGFTEIVHIDNHISFLENKKTGFQDYLMTLNKPEQTPGKVAAIVINANPFTLG
                     HQFLVEKAARENDWVHLFMVSEDQSLVPFSVRKMLIQEGLKHLDNIIFHETGPYLISQ
                     ATFPAYFQTEDNDVIKSQALLDTAIFLKIAQTLQITKRYVGEEPTSRVTAIYNEIMAE
                     QLQQAGILLDILPRKAINQQQDPISASTARQALKDSDWDLLAKLLPKTSLDYFCSLEA
                     QPIIKKIQATSSVKHY"
     gene            complement(28651..29133)
                     /locus_tag="VC394_000749"
     CDS             complement(28651..29133)
                     /locus_tag="VC394_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEP5994238.1"
                     /translation="MPKRIRKALGVVGKLMSIVPDTTEIIGKTIDNSRPIIEKRMEQK
                     HEKEMQLRTIDDVINLPVDQAQAHLEQLGFVVATIPAKPHKKWLHSNLNEVVAMSPKS
                     GKYKIGSLIKLYYITVDVLEKSQDLLDQENLRTVERNQKIADTFESVKHIRFPFKKWR
                     "
     gene            complement(29832..30902)
                     /gene="xerS"
                     /locus_tag="VC394_000750"
     CDS             complement(29832..30902)
                     /gene="xerS"
                     /locus_tag="VC394_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEP5994239.1"
                     /translation="MRRELLLEKIETYKAVMPWYVLDYYQSKLAVPYSFTTLYEYLKE
                     YKRFFDWLMDADLTQAPKIADIDLSTLEHLTKKDLEAFVLYLRERPSLNTYSTKEGLS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLASRAENIKQK
                     LFLGDETLAFLDYVDKEYEQKLSNRAKSSFRKNKERDLAIIALLLASGVRLSEAVNLD
                     LKDVNLNMMIIEVIRKGGKRDSVNVAGFAKGYLESYLAVRQRRYKAEKQDLAFFLTEY
                     RGVPNRMDASSIEKMVGKYSEDFKIRVTPHKLRHTLATRLYDATKSQVLVSHQLGHSS
                     TQVTDLYTHIVNDEQKNALDNL"
     gene            31372..32070
                     /locus_tag="VC394_000751"
     CDS             31372..32070
                     /locus_tag="VC394_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP5994240.1"
                     /translation="MFSGHQLKTARLSKGITQSELGRLLHVNKMTISNWEKGKNIPNE
                     KHLNALLHLFNVTSDYFDPNYRLLTPYNQLTISNKEKVIGYSERLLNHQIDKKSKDLI
                     DKPSQLYAYRVYESLSAGTGYSYFGDGNFDVVFYDEQLEYDFASWVFGDSMEPTYLNG
                     EVVLIKQNSFDYDGAIYAVEWDGQTYIKKVFREDEGLRLVSLNKKYSDKFAPYSEEPR
                     IIGKIIANFRPLEI"
     gene            complement(32205..33767)
                     /gene="ffh"
                     /locus_tag="VC394_000752"
     CDS             complement(32205..33767)
                     /gene="ffh"
                     /locus_tag="VC394_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEP5994241.1"
                     /translation="MAFESLTQRLQDVFKHIRGKKKLSESDVQEVTKEIRLALLEADV
                     ALPVVKTFIKRVRERAIGHEIIDTLDPTQQILKIVNEELTSILGSETAEIDKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLIKEENARPLMIAADIYRPAAIDQLKTLGQQINVPV
                     FDMGTDHSAVDIVRKGLEQARENRNDYVLIDTAGRLQIDEKLMEELRDVKALAQPNEI
                     LLVVDSMIGQEAANVAYEFNHQLSITGVVLTKIDGDTRGGAALSVREITGKPIKFTGI
                     GEKITDIETFHPDRMSSRILGMGDLLTLIEKASQEYDEKKSLELAEKMRENTFDFNDF
                     IEQLDQVQNMGPMEDLLKMIPGMAGNPALANIKVDENQIARKRAIVSSMTPAERENPD
                     LLNPSRRRRIAAGSGNSFVEVNKFIKDFNQAKSMMQGVMSGDMSKMMKDMGINPNNLP
                     KNMPAGMPDMSSLEGMMGQGGMPDLSGLGGDMDMSQVFGKGFKGKIGQFAMKQAMKRQ
                     ANKLKKAKKKRK"
     gene            complement(33782..34123)
                     /locus_tag="VC394_000753"
     CDS             complement(33782..34123)
                     /locus_tag="VC394_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEP5994242.1"
                     /translation="MNIMEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEI
                     ADEFGVSRQAVYDNIKRTEKILETYEMKLHMYSDYVVRSEIFDDMIAHYPHDEYLQEK
                     ISILTSIDNRE"
     gene            complement(34212..34910)
                     /locus_tag="VC394_000754"
     CDS             complement(34212..34910)
                     /locus_tag="VC394_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEP5994243.1"
                     /translation="MLPAYIKIHDAIKKDIDLGIWPIGSRLPSERHLAEHFTVSRMTL
                     RQAITLLVEEGILERRIGSGTYVASHRVQEKMRGTTSFTEIIRSQGRQPSSKLLSYQK
                     QLASDTEVKELNLDKTDLVIRMERIRYADSVPLVYEIASIPEKFIKTVKRADITEHFF
                     HSLIANGYEIGKSKQTIYAKLASERVASYLEVAKGHAILALTQVSYFTDGKPFEYVRS
                     QYIGDRFEFYLENN"
     gene            complement(34993..36426)
                     /locus_tag="VC394_000755"
     CDS             complement(34993..36426)
                     /locus_tag="VC394_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994244.1"
                     /translation="MLRKKRILGLFRPVELIFLGLLLSLVVSYLAWTNSFATLHNILA
                     TVGIVERSKDQQPRYHIGQAIQVQKSGPYHQWIGTINKQVEDIAENYRVSYHYEVVFP
                     IGKVTVSLPEHNLKEPDKPRFKKGDIVKLSSLTKKPHIKVYQGQLATIKQVKKRYDYS
                     LGGYQYDINLKDNLRLDGISEQDFVKPYYIRFNKGNSPEQNNRLLRKAFAYAKQHPNS
                     VISFPKGQFHIGSLPSQKDYFELPSDTAIIGHQTEFIIHGKMLWFGFPTGPKAEQGVR
                     NLVLTGVHFKANDLKKGDHFMIMADHGTDWHIYDNKFTMVHKRNSHIFDLGSLQNSLF
                     EKNQFIGYAPELVQDQQLLSKAQGHDFFSEVIQFDAAVHHFAWDGGLLSNIAPNYEAF
                     NQTRHLCHNITVSQNQFLPYIDPTGCLRAYSGSIGQHSSKVGVIRVLNNVFTSSIVTK
                     AKLTSWFMEPIHFPPNSPVIVAGNIIN"
     gene            36593..38155
                     /gene="guaA"
                     /locus_tag="VC394_000756"
     CDS             36593..38155
                     /gene="guaA"
                     /locus_tag="VC394_000756"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEP5994245.1"
                     /translation="MTEISILNDVQKIIVLDYGSQYNQLIARRIREFGVFSELKSHKI
                     TAQELREINPIGIVLSGGPNSVYADNAFGIDPEIFELGIPILGICYGMQLITHKLGGK
                     VVPAGQAGNREYGQSTLHLRETSKLFSGTPQEQLVLMSHGDAVTEIPEGFHLVGDSND
                     CPYAAIENTEKNLYGIQFHPEVRHSVYGNDILKNFAISICGARGDWSMDNFIDMEIAK
                     IRETVGDRKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHGLLRKDEGDQVMGMLG
                     GKFGLNIIRVDASKRFLDLLADVEDPEKKRKIIGNEFVYVFDDEASKLKGVDFLAQGT
                     LYTDIIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRALGIALGMPEEI
                     VWRQPFPGPGLAIRVMGAITEEKLETVRESDAILREEIAKAGLDRDVWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAQLPWDVLKKISTRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            complement(38197..39420)
                     /locus_tag="VC394_000757"
     CDS             complement(38197..39420)
                     /locus_tag="VC394_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyltransferase"
                     /protein_id="HEP5994246.1"
                     /translation="MTNLTFYAKIGISEEEHDSFVKEHQQISVLQGSDWAKIKNQWQN
                     ERIGIYKEEKQVASLSLLIKLLPLGRSIIYIPRGPVMDYLDRDLVAFTMKTLKDYGKT
                     KKALFIKYDPAILLKQYALGQEEEEKPLALAAIKNLQEAGVHWTGLTMEIADSIQPRF
                     QANIYTQENLEMQFPKHTRRLIKDAKQRGVETYRASQSELHKFSKIVSLTEKRKNISL
                     RNEAYFQKLMTTYGDKAYLHLAKVNIPQKLDQYRQQLNLINQDITRTQAHQKKRLKKL
                     EDQKASFERYITEFEGFTDQYPEEVVVAGILSISYGNVMEMLYAGMNDDFKKFYPQYL
                     LYPKVFQDAYQDGIIWANMGGVEGSLDDGLTKFKANFAPTIEEFIGEFNLPVSPLYHI
                     ANTMYKIRKQLKNKH"
BASE COUNT        12233 a         7480 c         7707 g        12052 t
ORIGIN      
        1 ttgtcacttt gttgaaatgt tttcgtatta atgttttttt atctattacg atagataatt
       61 ttacttcaat ttttgattga aaatactaaa ataagtgatg tggtcacttt gacaaccatt
      121 tattggtcct tttagataag acaattttgt ggctcttcag ttgaaaggat gtgttgttga
      181 tgttagagga aacaatcatg ttaaattaac tcaggcttaa agaccaaaaa aggagtgaag
      241 tctattatga aaaaaagaaa ttttaaactt acagaaatgt tacaattggt tttattagtg
      301 gttcctgctg taaaacttgc taaaaaaatg tttaacgata gtaaaacacc gtctaaaaga
      361 aagtaggtct acaatgacta aaaaaaatca tggcgttttt tgggggttgt tgcttggatc
      421 agcagcagct agcattgctt acttaagttt atcgtcttcc aaaaaagatc aactcttgaa
      481 agatagtgct aaaaaaatag atgatctcaa cgcttacttg caagataaaa gtaagcaagt
      541 tctggatgca gtatcggaaa aagtacaaga atccaaagat gctgttgaag tttatggtgg
      601 tattgccgca gaaaccgttg aagaaaagtg gcaagatacc aaagatgctg ttgaagttta
      661 cggtggtatt gccacagaaa cagttgaaga atctttggga caggttaaag aaaaggtaga
      721 aggaattggc gaagctacta gccaaactat ccaatctaaa atggaaaaac tcaccagcga
      781 taagacagat gctgatgatg aaaaataggt tttaaaatac ctttaagata agacaaaaag
      841 tgccattagg cactttttta atcttctgtt ggaacaataa tcgaaatggc ttttggaatg
      901 atatctaatt gaatgggcag atagtcactt ttatccccat caatcctggt acgtggtaat
      961 atcgcttgtt ttttagcttg tggtagtaag ctaagcgtat tggcttgaaa atgactgatt
     1021 tcctctgctt tggaaaactc accctgttta aaatcattaa tatgccataa aaatttaaag
     1081 aaagagactt ttttcatggt ataaacttgg aaatagccat cgtcagcttg agcaccagga
     1141 gaaaaagaag gaaaaccagc aatggtattt gtcatcgtga ttaagagaaa cttcgttttc
     1201 aggtgaatac gatggttgtg gctaattaaa gagatggcat agctgcgatt tcgtttcagt
     1261 attcgaatac tatcttttaa aaatgctaga ggaccaaagc gtcgtttttc ctcagccgtt
     1321 acgtcggcag caatatcagc cagtaatcct aaggttaaac tggaaatcat gtattgctgg
     1381 ttagctttgc aaatatcaac ttgcatgatt tgtccagtca agatagtatc caacgcctca
     1441 gtaatttgta aagggatatg catagccttg gcaaaattat tgactgtccc tgagggcact
     1501 aagcctatta aacaatgagc tcctccttca tagatgccac cgatgatttt atttagtgtc
     1561 ccatcgccac caagagggat aaccaagtca atcttgtcct tagcagcttg ttttgctaat
     1621 tgaaaagcct ggtcagcatc tttaggggta ataacttcta cagaatcttc ggaaaaacca
     1681 tgttggcaaa aatagtcttt gacttgccta gcaagttggt tttctttctt tccagaatta
     1741 ggattgtaaa aaatccgaac ggttttcatc aggtctctcc tttgacagct tattacttgc
     1801 tatccattat accacttcta agaattgatg tcttacatca aatcatcaca ttaagcctct
     1861 caatcagtct gagcatctat ttgtcttaaa gcataaagaa agtgatagcg ataaattgga
     1921 gggcagaggc caataaaata aagagatgcc aaatcatgtg gaagtagggt ctttttttgg
     1981 cataaaatac cgctccaatg gtataagaca agccaccaaa taacatgaga agaccaaaag
     2041 caagactagt tttttgaatg atgactggca agataaacac aacgagccag cccataacga
     2101 tataaagcat caaactgaat ttttcattga tacgtttggc aaaaattttg tataaaatcc
     2161 caaaaagcgt aatgccccac tgcaaaacaa taatgatata gcctagccaa ccagaaacaa
     2221 gggataaggc aacaggtgta taacttcctg cgatagcaat gtaaatcata ctgtgatcaa
     2281 taatgcgtag aatgtatttg tgaaccgaac cataagccat ggagtggtaa atcgtagagg
     2341 acaaaaacat caaaaagaga ctgatgacaa agatggagat accaatagcg gctttaaggt
     2401 cataagtttg ataagcatag ctggctgaaa taggaagtag aatgagcata gcaaatgctc
     2461 cgatagcgtg tgtcacgctg tttgctactt cctcactaaa agataagggt aaactttgtt
     2521 taaaggtttg gttcattgga tcacctcctg taataaataa atagttttgt ggtaatcttt
     2581 gaccgtttga caggcaacct gcacaatcgg tggaatgtcc tcctcaatgg gagccttctg
     2641 attccaacag gttacaaaag tattgtaaag actttcagaa tcagcctgtg cctgaagttc
     2701 ctccaataca cgactaatat cttgaatagg aaataccgtt ttaaacaaac tgatggcaat
     2761 taagcgcgct agttgttgct tttggtattt tttcttgata ggtttagtca catacccatg
     2821 cttgacgtaa ttattaatca tagatgccgt tagtgattta ttatctgaca cttctgaaaa
     2881 atcagtgcac tggttgacat aaagtaagac ttgatccaaa tataagtcta aatcagggag
     2941 gtccttccaa taaggtaatg atggaaaaat catagttttg tctctctatc tagttttgat
     3001 aactagatta tacgattaat gaaaacttat tgcaagcaaa aactgtaaag acttaaaaga
     3061 ggaaagtaga aatggcgaag tgaagctaat catgctataa tgttaaagaa ttgattggga
     3121 tagatgcctt ctgtcgcaat cagactattt tatcacgaag aagatgatgc tacgaaagga
     3181 atttaaactc tatggctact attcaatggt ttcctgggca catgtccaaa gccagaagac
     3241 aggttcagga aaatgtaaag catgttgact ttgtaactat tttagtggat gcacgcttac
     3301 cactttctag tcaaaatccc atgcttacaa aaattgttgg tgataaacct aaattaatga
     3361 ttttaaataa agctgacttg gcagatgcaa ctcgaactaa agagtggaag gcttattatg
     3421 aaagtcaagg tattaaaaca ttagccatca attccaaaga acaatcaacg gttaaaaaag
     3481 taacagaagc agccaaggaa ctcatggctg ataaaattca acgcttgcga gagcggggca
     3541 ttcaaaaaga aaccttgcga accatgatta ttggcattcc aaatgctggt aaatctacct
     3601 taatgaatcg cttggctggt aagaaaattg ctgtagttgg caataaacct ggtgtgacta
     3661 aaggacaaca atggttgaag tccaataagg aattggaaat tctagacact cctggtattt
     3721 tatggccaaa atttgaggac gaattggtgg gcttaaaatt agctttaaca ggtgctatca
     3781 aggaccaatt actaccaatg gatgaggtca ctatttttgg tttgaattac tttaaaacac
     3841 attatcctag tcgccttact aagcgtttta agaatattcc tcttgaggaa gaagctcctg
     3901 aaatcattat gaccttgaca cgccaactgg gatttaaaga tgattacgac cgtttctaca
     3961 ctttattcgt caaggaagtt cgtgatggta aattaggtca atacacctta gatcaggtgg
     4021 gagatatgga tgtcgactag tattaaagct attaaagaaa gcctggaagc tgttactagc
     4081 ctctcagacc ccctctttca agaattggca actgacgcta ggttaggtgt ccaaaaagcg
     4141 ctaaaaagtc gacaaaaggc tattcaggcc gatttagcag aggaagaacg attagaagcc
     4201 atgctttctt atgaaaaagc gctttataaa gaaggctacc aagccattgc aggtattgat
     4261 gaggtgggac gtggccccct agcaggtccc gttgtcgcag catgtgtcat cttacctcaa
     4321 cattgcaaaa ttaaaggtct gaatgattct aaaaaaatcc ctaaatctaa gcatgagacc
     4381 atttatcagg cagtgaaaga aaaggctttg gctatcggta tcggtattat tgacaatcag
     4441 cttattgatg aggtcaatat ttatgaagca accaaactag ctatgctaga agccattaaa
     4501 cagttggagg gccaactcac acaaccagat tatctcttga ttgatgccat gacattggat
     4561 attgctattt cgcagcagtc tattcttaaa ggcgatgcca attccttgtc tattgcagca
     4621 gcatcaattg tagctaaggt caccagagat cagatgatgg ctaactatga tcgcattttt
     4681 cctggttatg gcttcgctaa aaatgcaggc tatggcacca aagaacattt acagggatta
     4741 aaagcttacg gcataacgcc tatccatcgt aaaagttttg aacctgttaa atccatgtgc
     4801 tgcgattcaa ctaatcctta aaaagttctg tttttagcag aatttttttc ttttttacga
     4861 ataaatgaag taaggaggta acttagtgaa tcattttgaa ctttataagc taaagaaagc
     4921 tggactgact aataaaaaca ttctcaatat tcttgactac caagaacacc aggaaaaatc
     4981 gttgtcactt cgagatatgg ccgttgtttc tggttgtaag catccgtctc actttataga
     5041 agcctataag cagttagata ttcaaaaatt aaaaatagaa tttaaacaat ttcctagtat
     5101 ttctatctta gataagcatt acccaatggc attgaaagaa atatacaatc cacctgtcct
     5161 cttgtttttt cagggaaatt tagaccttct tgagaaacct aaattagcca ttgtcggctc
     5221 cagacgctca agcgataccg gagtaaagtc tgtccgtaaa attcttaaag aactcgggaa
     5281 ccgttttgtg attgttagcg gacttgctcg aggtatcgac actagtgctc atttagcctg
     5341 ccttaaaaat gggggacaaa ctatcgctat tattggaaca ggattggatc gcttttaccc
     5401 taaagaaaat agggagttgc aaactttctt agggaaaaat catcttgtgc taacagaata
     5461 cggtccagga gaagaagctt tatcttatca ctttccagaa cggaatcgta ttatcgcagg
     5521 ccttagccga ggtattcttg tcgttgaagc aaaaaatcgt tcaggttcct tgattacttg
     5581 tcaaattggg atagaagaag gccgagacat ttttgctgtc ccaggaaaca ttttggacgg
     5641 gaaatccgaa ggttgccttc agttaattaa agagggagca acatgcgtca catcgggcat
     5701 ggatatcctt tcagagtact aaaaaagtta aagtttcttc ctatatagca tttttttatt
     5761 gacagtttct taaaaatggg atattatgct ataagcttta aacaaactgt tataggaagt
     5821 gtgtcgaatt gggaacaaaa actacaacta agccaaaaac gggtactaaa aagtccacca
     5881 ctaaaaagaa atcaacagcg aaaaaaaatc tcgttattgt tgaatcacct gccaaagcta
     5941 agactattga aaaatatctt ggacgcagtt ataaagttgt tgcttcggta ggacatattc
     6001 gtgacttaaa aaaatcgtct atgtctattg attttgataa taattatgag ccacaataca
     6061 ttaatatccg tggtaaggga tcccttatca attctttaaa aaaagaagcc aaagctgcta
     6121 aaaaagttta tttagctagt gacccggatc gtgagggaga ggctatttct tggcatttgt
     6181 cacatatctt aggacttgat cctcaagata ataaccgagt ggtttttaat gaaattacga
     6241 aagatgcagt taaacatgcc tttgtggagc ctcgtcagat tgatatggac ttggtagatt
     6301 ctcaacaagc acgtcgtgtt ttggaccgta ttgtgggata ttccatttca ccaattcttt
     6361 ggaaaaaggt caaaaaagga ctttcagcag gacgtgtcca gtcggttgct ttaaaactca
     6421 tcattgaccg tgaaaatgac atcaaagcat ttgttccaaa ggaatactgg tctattgatg
     6481 gtctttttaa aaaaggaact aaaaagtttc aagcgacttt ttacggtatt aatggcaaaa
     6541 aaacaaagct tgataataac aacgatgtca aagaggtatt agcaaagcta acaaatgaag
     6601 attttcttgt cagcaaggtt gacaagaaag agcgacgtcg taatgctccc ttaccttaca
     6661 caacatcatc tcttcaacaa gatgctgcta ataaaatcaa tttcagaacg cgtaaaacca
     6721 tgatggtagc ccaacaactg tatgagggga ttcatttagg agaaaatgga actcaaggtt
     6781 taattaccta tatgcgtacg gattctactc gtatcagtcc agtagcacaa aacgatgctg
     6841 ctcaatttat tatcaatcgc tttggagcta attattcaaa acatggtaat cgtgtcaaaa
     6901 ataccagtgg tgctcaagat gctcacgagg cgattcgtcc atctagtgtt aatcatacgc
     6961 cagactctat tgccaaatac cttaacaagg atcagttaaa actttatacg cttatttgga
     7021 atcgttttgt ggctagccaa atgacagcag ctgtttttga taccgtaaag gtgaatttgg
     7081 agcaaaatgg cgtgattttt gttgccaatg gtagccaaat gaagtttgat ggttacatgg
     7141 ctgtttacaa tgattccgat aaaaacaaaa tgttacctga gatggcagaa ggagaaacgg
     7201 ttaaaaaaat atcgacctct ccagaacagc actttacgca gccaccagct cgttattctg
     7261 aagcaactct gattaaaacg ctagaagaaa atggtgttgg acgtccatca acttatgccc
     7321 caaccttaga agtgattcaa cgcaggtact atgttaaatt atctgctaag cgctttgagc
     7381 caactgagct tggggaaatt gtcaacaagc ttattgtcga atttttccca gatattgttg
     7441 atgttgcctt tactgccgag atggaaggta agctagatca agttgaaatc ggagaagaac
     7501 aatggcaaca tgtcatcgat caattctatc aaccctttgt caaagaattg aataaggctg
     7561 agtctgaaat tgaaaagatt caaatcaaag atgagccagc tggttttgac tgtgatgttt
     7621 gtggccaccc aatggtcatt aagttaggac gttttgggaa attttacgcg tgcagcaact
     7681 tccctgagtg tcgtaataca aaagccatca ctaaagaaat cggtgttact tgtccggttt
     7741 gtcataaagg acaagttatc gagcgtaaaa ccaaaaaaaa taggatattt tatggttgtg
     7801 atcagtatcc agattgtgag tttatctcgt gggatttacc tattggacgt gcttgcccta
     7861 agtcaggtga ctatcttatt gagaaaaaag ttcgtggtgg caaacaagta atgtgtagca
     7921 atgagacctg tgattacaag gaagaaaaaa ttaaataaaa aaagaattgg aactccttgt
     7981 cttgttccaa ttcttttttt atttacaaag gtatttttaa ctacaaaaat cgctagttcg
     8041 tttttaaaag gcttaaaaat tcctcaataa ctggactgaa actcgcatca tctcggtaag
     8101 caatcccaat agggtaaaaa ttatttttat cttgcaaagg aatccaaact aagttatcta
     8161 cctgattaag actctcaaaa tcactcggga ggatagtcaa ggtatttaaa tcgtgcaaac
     8221 tatgaataag aacttcccaa tcattgtgtt taaagacaat atctggttca taaccgagag
     8281 ctcggcattt tctaggaagc atttcgccta acatgtagtg atcgtttaaa gaagcaatct
     8341 tatagccttt taaatcttta agttttaact gaggatgttg cgccaaaggg tgctgtttga
     8401 ggagaacgat actgacctta tagccttttg tggacgtttg taatagctca atggtaatat
     8461 ccttttgaat acttagaaag gagaggagac caatatcgat ttttccttgg gacaataatt
     8521 cttataattt gcgagaacca tcctgtataa gcgataattc aacatttgag tgagtagcca
     8581 taaagattga aatttgtttc atgaattgta tagcaaacag tgatattaat tcaacacgaa
     8641 tcatctcttt ttttcttgat ttaattgctg aatatccgca acaatttgat taaagtcatc
     8701 cacaaggtct ttgcctttgt gataaagaat atctccagct tcggttagta gtagctgtca
     8761 atcactttgt gtaaaaagcg tcgtgttcaa actaggactc tagcctttta attgattgag
     8821 aaagagtatg ttgcgttaca aacaaacttt tagcagcgtg ggaatgattt tttgtttcag
     8881 caactgcaat aaaataggtt aattatctaa tatccatagt agatccttca tgattggtca
     8941 gggaattgcg tctctacttg gtttacaacc tattttgatg tctctcctta ttgccatgat
     9001 tttctgcttc cttattgttt ctccaattac aacggtagga attgctcttg cgattaactt
     9061 atctggtatt ggttcaggtg ccgcaagctt tggactttgc ttagcaggtt gggctgttaa
     9121 ttcaaaagga acttcacttg ctcacgtctt gagatcaccg aaaatttcaa tggccaacgt
     9181 tttatcaaaa ccaaaaatca tgttgccaat gttatgttct gcagctgtac ttggtgttat
     9241 tggtgctatc tttaatattc aaggaacacc tgctagtgca ggctttggaa ttagtggttt
     9301 gatcggacct atcaatgccc ttaatttagc taaaggcgga tggtctccag taaatattct
     9361 acttatcatt atcatttttg taggggcacc cattgtgctt aatatgatct ttaactacct
     9421 ctttattaag gtgcttaaag tgattgatcc aatggattac aagcttaata tctaagaaag
     9481 gaacttttga tgaaattaaa attatataat gttagaggcg aagaagcagt cttagcaaaa
     9541 aaatgggctg atgacaatgg gattgagatt tccttgacag agtctccctt aacccctgaa
     9601 acggttaaag aagctgaagg ctttgacggg attgccaatg cccaaattgg tcctttagat
     9661 gatgcaatct atcctctctt aagggaaatg gggattaaac aaatcgctca gcgcagtgct
     9721 ggtgtcgata tgtataacct tgatttggcc accgaaaatg acattattat cacaaatgtt
     9781 cctagctatt ctccagaatc cattgcagaa tttacggtta ctattgccct taatttaatt
     9841 cgtcatgtgg agttgattcg tgaaaatgtt aaaaaacaaa atttcacttg gggactccct
     9901 atccgcggtc gtgttttagg tgatatgaca gttgcaatta tcggaactgg acgtattggt
     9961 ctagctactg ctaaaatctt taaaggtttt ggctgcaagg tcgttggata tgatatttac
    10021 caaagcgatg ctgccaaagc tgtcttggac tataaggaat ctgtagaaga agcgattaaa
    10081 gatgctgatt ttgtttcctt acacatgcca ccaactgcag agaacacaca ccttttcaat
    10141 gctgatttat ttaaatcatt caaaaaaggg gctattttga tgaatatggc gcgtggtgcc
    10201 cttattgaga cgcaagattt actggatgct ttggatgcag gcttattgag cggagctggt
    10261 attgacactt acgaatttga aggaccttat attcctaaga attttgaagg tcaagaaatt
    10321 acagattcac tatttaaagc tttgattaac catcctaagg ttatttatac gcctcatgca
    10381 gcttattata ctgatgaagc cgttaaaaac ctagttgaag gtgcccttaa tgctacagta
    10441 gagattatca aaactgggac aacgacaact cgtgttaact aacaactgat ttgacgtcca
    10501 ttataaagga ataattccaa aaattccttt ataatggact tttttgcatt agtccgttat
    10561 aatagactgt aagaaagggg gctcttatga catatatagc gcttattgga gatattatcc
    10621 agtccaaaca attgacggat cgttccaaag ttcaaaaaac tttagcagct tatttagacg
    10681 acctcaacaa aacctttgct ccttatatta tttctaaatt atccctaacg ttgggagatg
    10741 aatttcaagg actttttcag gtggatactc ctattttcca tctgattgat ttgattaatc
    10801 atcacatgga tatccctatt cgatttggag taggagtagg ttctattcta acagacatca
    10861 atcctgatat tagtattggg gctgatggac ctgcttattg gcatgctcgt gaggctattc
    10921 gttatattca tcaaaaaaat gattatggta atactacatt agcttttcgg actggtcatc
    10981 acaaccagga tgatgtatta aacagtttac tagcagctgg agatgctatt aaagccaact
    11041 ggcgagctag tcaatgggaa atttttgaca cgcttttaga tttgggcatt tatgaggaat
    11101 actttgatca acaacgacta ggaaagcaat tatctcttag ctctagcgct ttgtcaaaac
    11161 gtctaaaaag tagtcatgtt aaaatttatc tgagaacacg gcagagtgcg ctaaactgtc
    11221 tcaaacaaat caaggaggaa actgatgatt aatggtgtct ctcactacct agctcaaacc
    11281 cctacactta ccctgtttct catttgtcat gtgttatcag actaccagtt acaaagtcag
    11341 caagtagctg atttaaaaga aaaacatttg acttatttag gttaccactt gattggagtt
    11401 agcattcccc taatttgttt aaccctcatt atcccacaag catggttgat gagcctattg
    11461 gttatgataa gtcacgcgct tattgactgg ctaaagccaa agatggctaa ctctctaaaa
    11521 tggaaaagag agtggatttt tctgctggat caatgcttac atatcgctat tagttcattt
    11581 gctgggctaa ggttagcagg ggtgaccttg cctaattggc ttccgatatc tattttgatg
    11641 accgttttat ttatcctact cattaccaaa ccaactaata ttgtctttaa actctttttc
    11701 agcaaatacc agcctgacca aggagaaaaa atggacacga tcataggagc gggggctacc
    11761 ataggaattc tagagagaat tgttattggg gtgtgtatgt tcatggggca atttgcttca
    11821 ataggtctgg tctttaccgc taaatctatt gctcgctata ataaaatttc ggaaagtcca
    11881 gcttttgcag aatactacct aattggttct ttatttagta ttctcagtgt ttttatcgca
    11941 gcatggattt gtttcttcta gttgaaaagg gttcccgtag aaagaggcta cacatagcct
    12001 cttttgcttt tttccccttt ctgttataat aagtcaggta actacattag aaagatttgt
    12061 gggtctgtca tctaagagat atcattcccg aagaggtatt agtgacttgt ctcaatcaac
    12121 tgcaacttat attaatgtta ttggagctgg gctagctggt tctgaagctg cctatcagat
    12181 tgctaagcgc ggtatccccg ttaaattgta tgaaatgcgt ggtgtcaaag caacaccgca
    12241 acataaaacc actaattttg ccgaattggt ctgttccaac tcatttcgtg gtgatagctt
    12301 aaccaatgca gtcggtcttc tcaaagaaga aatgcgtcgc ttggattcca ttattatgcg
    12361 taatggtgaa gctaatcgtg tacctgctgg aggagcaatg gctgttgacc gtgaggggta
    12421 tgcaaagagt gtcactgcag agttggaaaa tcatcctctc attgaggtca ttcgtgatga
    12481 aatcacagaa atccctgacg atgccattac ggttatcgcg acgggtccat tgacttcaga
    12541 tgccttggca gaaaaaattc acgcgctaaa tggtggcgac ggattctatt tttacgatgc
    12601 agcagcgcct atcattgata aatctaccat tgatatgagc aaggtttacc ttaaatctcg
    12661 ctacgataaa ggcgaagctg cttacctcaa ctgccctatg accaaggaag aattcatggc
    12721 tttccatgat gcactaacaa acgcagaaga agcaccgctg aatgcctttg aaaaagaaaa
    12781 gtattttgaa ggctgtatgc cgattgaagt tatggctaaa cgtggcatta aaaccatgct
    12841 ttatggacct atgaaacccg ttggattgga atatccagat gactatacag gtcctcgcga
    12901 tggagaattt aaaacgccat atgccgtcgt gcaattgcgt caagataatg cagctggaag
    12961 cctttataat atcgttggtt tccaaacgca cctcaaatgg ggtgagcaaa agcgtgtttt
    13021 ccaaatgatt ccagggcttg aaaatgctga gtttgtccgc tacggcgtca tgcaccgcaa
    13081 ttcctatatg gattcaccaa atcttttaac cgaaaccttc caatctcgta acaatccaaa
    13141 tcttttcttc gcaggtcaga tgactggagt tgaaggttat gtcgaatcag ctgcttcagg
    13201 tttagtagca ggaaccaatg ctgctcgttt gttcaaaaga gaagaagcgc ttgtttttcc
    13261 tcagacaaca gctattggta gcttgcctca ttatgtgact catgccgaca gtaagcattt
    13321 ccaaccaatg aacgtcaact ttggcattat caaagagtta gaaggcccac gcattcgtga
    13381 caaaaaagaa cgttatgaag ctattgctag tcgtgctttg gcagatttag acacctgctt
    13441 agcgtcgctt taacactaaa aaagagttcc ttttcagttg agttgctgaa gaggactttt
    13501 ttccttgctg atttgagcgc tagctatgca aaagaatgca caaaaaatac atgaatagac
    13561 tatggtcact agaagcaata ttatataatg aatagtactc attagttgat tcagtttgga
    13621 ggttttagca tgcagcagca agtcgcaata acagaaacag ttttacggga cggtcatcaa
    13681 agtttgatgg caacgcgttt atctattgag gatatgcttc ctgtccttac catcttagac
    13741 aagattggtt attattccct agagtgttgg ggaggagcaa cctttgatgc ctgtattcgt
    13801 tttttgaacg aagacccttg ggaaagactg cgcacgttaa aaaaaggctt acctaatacc
    13861 cgtttacaaa tgttattaag aggccaaaac cttttaggct accgtcatta tgcagatgac
    13921 atcgttgaca aattcattag tttatcagct caaaatggga ttgatgtttt ccgcattttt
    13981 gatgccctaa atgacccacg aaatatccag caagccttaa gagctgtgaa aaagacgggt
    14041 aaagaggctc aactgtgtat tgcctataca actagcccag tgcacacgct taactattac
    14101 ctttctttag tgaaagaatt ggttgagatg ggagctgatt ctatctgcat caaagatatg
    14161 gctggtattc taactcccaa agcggctaaa gagttagtat ctggtatcaa agccatgact
    14221 aacttacctc ttattgtgca tacccatgca actagtggca tttctcagat gacctatcta
    14281 gcagcagtag aagctggtgc tgatagaatt gatactgctt tgtcaccttt tagcgaaggc
    14341 acttcacaac ctgctacaga atccatgtat ttagccttaa aagaagcagg ttatgatatc
    14401 acattagatg aaacgctact ggaacaggct gctaatcacc ttcgacaagt ccgtcaaaag
    14461 tacttagcag atggcattct ggatcccagt cttctctttc cagacccaag gacactacaa
    14521 taccaagttc ctggaggcat gttatctaat atgctatctc agctgaaaca agccaatgcc
    14581 gaatcaaaat tagaagaagt tttagcagaa gttcctcggg taagaaaaga cttgggttat
    14641 cctcccttag taacaccgct tagccaaatg gtcgggactc aagctgctat gaatgtgatt
    14701 ttaggaaaac cttaccagat ggtgtctaag gaaattaaac aatacttggc aggcgactat
    14761 ggcaaaacac cagcaccagt caacgaagac ttaaaacgaa gtcaaattgg ctcagctcct
    14821 gtcaccacaa atcgtcctgc agatcaacta agtcctgaat tcgaggtttt aaaagcagaa
    14881 gtagcagatt tagcccaaac ggacgaagat gtgctgactt atgccctctt cccaagcgta
    14941 gcaaaaccgt ttttaacaac caaataccaa actgatgacg tcattaaagt gactgccttt
    15001 atcaaagcat aataggctat cttaatcaga tagaaacaaa aatatggaga tcaaatgacc
    15061 tattccttta ttgatatctt acaagttacc ttagtatcct tgttcttagt gttaacctgt
    15121 ttaacaggga ttatgggatt aatgatgcta agtggtaaat tattgtcaca agaaaaaaaa
    15181 actctctctc aacaagcttc cagcattcct caagagcaag aagctgacaa gttgagtgga
    15241 gaagctctcg aaactctttt agccaaagac gaattggcta gaattgctgt tatgactagt
    15301 cttgcttatg ctagccaaga agagtcagga aaacggtttg aagttattgc cgtggaaagg
    15361 aaatcctaat gaaaagatat gaagtgactg tcaatggaca ggtttatgaa gtgagtttac
    15421 ttgaactagc tgatggagaa actgttcaag ttagccggcc agcagcacct gctactgaga
    15481 aagaaatgaa tgccaacgct gctggcggtg gcctacaggt aaaggctcca atgtctggaa
    15541 cagtcttgtc tatctttgct acagaaggaa aagctgttaa aaaaggagaa gctgttctgg
    15601 tcttagaggc catgaaaatg gagaatgaaa ttctagcacc tgcagatggt ctggtttcaa
    15661 aaattcatgt tgtggctaac caaacggtcg agtctgaaca agttttgatt tcattctagg
    15721 aggaaaccat gctagacgta ctcaatcaaa tggtccaatc gtcaggattg gctcatctaa
    15781 ctgttaataa tcttatcatg atttgcttgg cctgtttctt tttatacctt ggtatcaaaa
    15841 aggaatatga gccttacctt atggtaccaa tcgcctttgg gattttactc gttaatttgc
    15901 ctatggcggg attgatggac caccctgcca atggtaatcc tggtggcctt ctttattacc
    15961 tatataaggg aactagccta gggatttacc cacctttaat ttttctttgt ttgggggcat
    16021 caactgactt tggtcccttg attgccaatc ctaaaaccat tttactagga ggcgcagcac
    16081 aagttgggat tttcttagcc tttttccttg ctattatgtt aggcatgaca cctcaagaag
    16141 cggcttctgt cgggattatt ggtggtgccg atggaccaac agctatttat gtgacgacaa
    16201 agttggcgcc tgatttactc tctactatcg ccctagcagc ttattcttac atggctctgg
    16261 tacctattat tcaacctcct atcattaaac tcttgactac caaggcggaa cgccaagtga
    16321 aaatgactca agcaagaact gtttcacaaa aagaaaaaat cattttcccg attatggtaa
    16381 caatctttgt cagccttttg gttccaagtg ctacaacctt ggttggctgt ttgatgctgg
    16441 gaaatctggt cagagaaatt aaaattgttc ctaaaattgt agagaatctc caacaagtgg
    16501 ttatgttttg cattactatc attttaggat taaccgttgg tgctaaggcc aatggagatc
    16561 tctttttatc agccacaacc ttgaaaatta ttgcccttgg gctaatcgcc tttgctgctg
    16621 gtacagcagg gggtgtcttg atggggaagg tcatgtacta tttatctggt aacaaagtga
    16681 accctatgat aggagcagca ggtgtttcag ctgttccgat ggcagcgcga gtggtccaaa
    16741 aggtcggaca agaagaagat ccaagtaatt tcttactcat gcacgccatg ggacccaatg
    16801 tagctggcgt tattggctca gccattgctt caggtgcctt gctggccttc tttggctaat
    16861 tgtgaaaaag gattagtaaa ttaatcttaa taaatcatta tcaggagttg gaagcaatct
    16921 agctcctttt ttagtagaaa gctcgagtga taaactattc caaaataatt ataaaaaaag
    16981 actgtcctga ttgtacggac tatatcaatt gacttttata gtctagctga aatagcagtg
    17041 caaaactaag acactcgttt taaagtagtt tttctaaaaa agcaaaataa aaggtcagag
    17101 ccaataaatc tgccgcgccc ccagggctta aatgctgatt aatgaggcat tgattgtagg
    17161 aggacaaagc caatcttagg tcagttgttg ataaatcttg ttgcaggagt agtcgcatat
    17221 ctgctttaac gcctttccat gcttcaatcc ctccacgatg aatcagattt ccatcttcaa
    17281 caatagacat caaataaaca agaagacgaa gctgactggt ttcggggtca ttttgactta
    17341 tcatctgacg aaagtagggc aaggcatggt ctgttaaggt cgtgaatccc tcgcttgcct
    17401 caccccgagg tcctttgatg ccataagtta caaagagttg ctccccataa gtgaactctt
    17461 ttttagtgtt caaatgacca aggtctgttt gaatcaaatg tgccgtcata ggttttacta
    17521 aacgacagat agctagagta tcttccttgc taaaacaccc taaatcagtc attaagtgag
    17581 gtgttcttgc tagataggcc ccagttgcac caagaagtaa agccatggaa aaattcaggc
    17641 ctttatgagt gttaattccc tgagtagcac aaaacatggt ttcctcggcc ttttgaccta
    17701 gttgtctaag acggttaaac agaagaagtg gctcttcttt tgcataagca aaacctgttt
    17761 cgatataagc ctgaaaaaaa ggagagagtg ctatcatact gtcgataaag gtcataaagg
    17821 acatgtcatc atgggcaccg ttatcaaatc gatcaactaa accaggtttt ggagatagtg
    17881 agacttcata aagcaaagct tttaaggcca gttgactaat acttgttaga actgccttag
    17941 tcattttgct caggcctcat ttcttttaag ataaattcaa gtgagctatt caagtgttca
    18001 tgtgtaatca acgtaatttg ctcatgatct ttgttcttca tacctcggta aatcatccag
    18061 tgttcagata gagctcgttt acggcgttca actgaagaaa tcgaaatttt tctaaagtag
    18121 accaaataag cttgcaattc tgtcacaatt tcttttaagc ggaccatttg gctatagctg
    18181 taaatcaaat tgttaaaggc attaaaatta tccaaaatac gattggtgtc atcctctgcg
    18241 ataaaggttt cacagtcttc caacaagtta tgcatgactt taaaatcttc ttctgtcatt
    18301 aaatgcatcg cttgcactgt tgcgagagtc tctagagctt ttcgaatttc aaaaatttca
    18361 caggcatcct tgatactaac tcctttgaca ataatgcctt ttttaggaat gtgctctaca
    18421 aggtgttctt cagacaataa tcccaaagcg tagcgaatag gtgtcctgct aatattaagc
    18481 gcaatggaaa actctttttc attaatgcgg cttccaacag gaatttgtcg caaaataatg
    18541 gtcgttttaa gagcgttata aaaggctatt ttcaaaggga tatttcttga taggtcaaga
    18601 ttttgtttaa cggcttcgat aataggattc atgtcatctc cttaatgcta gtgtcctcac
    18661 tttattatac ttaaatttgt gaaacattcc aataggacta gatgagttct gaaaaattaa
    18721 agccatgctc acaaaaggac atgactttaa tcatctcata aggaataagg tagtagtgtt
    18781 atcgttggtt tattgtggaa tgtaaagtgg catttgcccc atggcaataa tggttaccac
    18841 aaagataaca aagatgataa gggcatattt agcagcctct ttttgccatt cccccatgtc
    18901 aagacctgtc aagcgaagaa gaagataaat gaaggcaact aatggactaa gtaagtggaa
    18961 ggcttgtccc atgagggaag caagtgccat agccatgtta ctgaaaccat attcagcacc
    19021 agcttctgca agaacaggca agataccata gtaaaaacca tcattggaga taaagaaggt
    19081 accaggtgca gaaactaagg caataacaag accccagaaa cctgccagtt gttttggaat
    19141 aatggttgca aaactttgtg caagagcgct agccatacca gaaccttgga aaagacccat
    19201 aaagatacca gcagcaaaga ctaagataac cacttgaacc gcatcaccag cattgtcacc
    19261 aatgcgtttt gattgatctt taagtactgg gtaattgacc atcaaggcga tacaagttcc
    19321 caccgcaaaa agaagaagcg gtgccatagc aatagatttg ttgaaagagc cagcaactaa
    19381 ccaagtgatt aatccaatgg ttaggatagc attgaaaaca aagtttttag gacgacgaat
    19441 aaaaagggta tctgggtcag taatgtctgt gagttgacgc atgtcttctt cagacaagtc
    19501 aataacacca agtcttgcac gttctttacg ccccatgctt ggagcaaccc aacaaatcac
    19561 ataaagaaga gataaaatca tacccggtgc aagatagcca agaatttcag gaccgacacc
    19621 aagaacagac atcgcacgag cagtagggcc accccatggc agtaagttca taatagtatt
    19681 ttgaagaata atcaagacac ctaggttcat gattttcatg tccaattttt tatagatagg
    19741 taagaaagca gagcaacaaa ttaaagtagt ggttgttcca tcaccattaa gagatactgc
    19801 agcagcaacg acagctgttg cgataagcac cttcattgga tcacctttag caaagcgaat
    19861 catcttttca gtaatcggat caaacaatcc tgcatctaac atgacagaga agaacaaaat
    19921 ggcaaatagc aacatgatac ctgttttagc agtattgttg ataccataca taaccattgg
    19981 tccgatagct gttaggtctt tagcaattcc accgtcacca acaaaggcaa caaatttggc
    20041 atctgcattg aagtcagctg aaccagtcag tataacagca atcgtcatga ttaatggaat
    20101 catgaccaaa gcagtaaaag gggtcatttt tttcttcatt accacgtaca taaagacaat
    20161 aatcatggca taagccagca ttgttaataa catcattgtt atctcctttt tcaagttatg
    20221 atttcagtat agatgaaagc gcttataaaa taaatacttc tttgtgcaga aagacacaaa
    20281 aaaaacaatt tgaaaattaa ttttgaaaac catttttatt acatcagatg actttaaatt
    20341 ttctataata atgatgaacc tagttagatc aagcttttct tgctatcagt atcttgatct
    20401 cagtttttca acagttacct aaaaataaaa taaaaggtaa ctgttgaaaa actttatgaa
    20461 acattgaaag cgctcttaga aagaacacaa aaaagccatc tcaaaaaaga ttggtagtat
    20521 ataataaact tactagttcc ctaaataggt aaatgtgaaa aagaggtagc tcatgcagtt
    20581 tttacaaaaa tggtttaaac actctaaaaa agagaagttg aaagaatcct cgccccttag
    20641 tacagaaata gaaccttctg aaaactggga aaagattccc gcttatattc ctgcagacaa
    20701 aagtgattac aaaaaggtta ctttaattac cagtgctatt gctgctggcg atagacctaa
    20761 tagtcaattc aaggttaaac gcattctcaa gcgcaatcct gaggcaatca ctgtttcttt
    20821 aattgcttca agtatcgctg caggtgttta cccagaaagt cagtttcggg tgacatccat
    20881 ctattgcaaa aggtagtcgt tgaacgatac tttagacgaa ctcatgacac aataacatat
    20941 aggagaaaaa tgatgttacg aaaatttaaa attaccattg acggtaaaga atatcttgta
    21001 gaaatggaag aaatcggtgc tcctgcccaa gcagctgccc ccgctcaacc aatatccact
    21061 ccagtacctg tccctactga agcaagccca caagtggagg aagcgcaagc accacagcca
    21121 gtagctgcag cgggagcgga tgccattcca tcaccaatgc ctggaaccat cttaaaagtc
    21181 ttagtagcag tgggagacca agtaactgaa aatcagccac tattgatttt agaagccatg
    21241 aagatggaaa atgagattgt ggcctcatca gcaggaacca ttacagctat ccacgtcggt
    21301 ccaggtcaag tggtcaatcc tggtgatggt ctcattacaa ttggttaata gtactataac
    21361 tagaataaat agacagaaaa ggaagtgaat ttgtggacac tttaatcgca ggaataacct
    21421 ccatcaccat tcctcaaatt gttatgatgg tcatcggtgc cctcttaatg tatctaggaa
    21481 ttaaaaaaga atacgaacca accttacttg tccccatggg acttggaacc attctcgtta
    21541 acttccctgg ttcaggagtt ttaacccaag tggttaacgg agtcgagcaa gaaggggttt
    21601 ttgaggctct cttcaatttt gggattggaa cagaactttt cccactgttg attttcattg
    21661 gtattggcgc catgattgac tttggtccct tgcttcaaaa cccattcatg ctcttgtttg
    21721 gtgctgcagc gcaatttggg attttctttg ttgttgttgt tgctgtactg gctggctttg
    21781 acattaaaga agcagcttca atcggcatta tcggagcagc agatggaccg acttctatct
    21841 ttgttgccaa ccaactggcc aaggatttac tgggtccaat tacggttgca gcctattctt
    21901 acatggctct ggttcctatt atccaaccat ttgccattaa attagtgaca acgaaaaaag
    21961 agcgccgtat ccgtatgact tacaaagctg agaatgtatc tcaaatgact aaaatcttat
    22021 tcccaatcat cattacctta gtagcagggt tcatcgcccc aatttctcta cctttagttg
    22081 gctttttgat gttcggtaac ttattacgtg agtgcggtgt gctcgatcgc ctgtcacaaa
    22141 ctgctcaaaa tgaattggta aacattatta gtatcttgct tggattgacc atctctatta
    22201 aaatgcaggc agacctgttc ttaaatgtac agacactctt aattatcgtc tttggtttac
    22261 ttgcttttat catggattct atcggtgggg tcatgtttgc caaatttctc aatctcttcc
    22321 gaaaagagaa gattaatcca atgattggcg ctgcgggtat ttcagctttt ccaatgtcaa
    22381 gtcgtgtcat tcaaaaaatg gcaacagatg aagacccgca aaactttatc ctcatgtatg
    22441 ctgtaggtgc caatgtctct ggccaaatcg cctctgttat cgcaggtggt ttgttgctag
    22501 cttactttag ctaagaaagg aaggaaagct tatgacaatt aatatggacc atttgattag
    22561 ttcctttgag ctaatggcgc ttggtatggc aggtgtcttt atcgtgttag gaattctcta
    22621 tcttgtcgca gagatcctga tcaaactttt cccagttagt aaataacaat atcatttaag
    22681 aaaatcaagg aggtaaacgc aatggatatt aaacaaactg ccgttgctgg ttcacttgaa
    22741 tcaagtgacc taatgattac agtatcccct aatgacgaga aaaccattac aataacccta
    22801 gacagtagtg ttgaaaaaca atttggcaat cacattcgtc aactcattca tcaaacccta
    22861 gtgaatttga aggtgacggc tgctaaagta gaggctgttg ataaaggcgc tttagattgc
    22921 accattcaag cgagaaccat cgcagctgtt catcgcgcag ctggtgttga ccaatacgat
    22981 tggaaggaga ttgactcatg gaacgtttaa gaagaacaat gatgtttgtg ccaggtgcca
    23041 atgctgctat gcttcgagat gctcctttgt ttggtgctga ttcaatcatg tttgacttgg
    23101 aagattctgt ttcactcaaa gaaaaagaca cttctagagc tctcgtccat tttgcgctaa
    23161 aaacttttga ttattcgagc gttgaaacgg ttgttcgtgt gaatggtctt gattcttgtg
    23221 gggctttaga catcgaagct gttgttttgg caggtgtcaa cgtgattcgt cttccaaaaa
    23281 cagagactgc acaagatatc gttgatgtgg aggctgttat tgaacgtgtc gaacgtgaga
    23341 acagcattga agttggtcgc acacgtatga tggcagccat tgaatcagcc gaaggtgttc
    23401 taaatgctcg tgagattgcc aaagcttcta agcgcttgat tggtattgct cttggggcag
    23461 aagactatgt cacgaatatg aaaacgcgtc gttacccaga tggtcaagaa ttattctttg
    23521 ctcgtagcat gattttacat gctgctcgtg ctgctggaat tgctgccatt gatactgttt
    23581 attctgatgt caataataca gaaggattcc aaaacgaagt tcgtatgatc aaacagttag
    23641 gatttgacgg taaatcggtc atcaaccctc gccaaattcc tctggtcaat gagatttata
    23701 ccccaaccga aaaagaaatt gaccattcca aacaagtcat ctgggcaatt cgtgaagctg
    23761 aaagcaaagg ctctggcgtt atttccttaa atggaaaaat ggttgataaa ccaatcgttg
    23821 aacgtgcaga acgtgtcatt gccctagcaa cagcagcagg tgttttatct gaggaggaca
    23881 tttaaagatg gtaacaaata aactcggtag agatattcct caaccatacg ccgaccaata
    23941 cggtgtcttt gaaggagaac tcgcaaatat caaacagtat gacgaatcaa gtcgccgtat
    24001 taaaccagtt aagcctggag attctaaatt actaggatct gttcgtgaag cgattgaaaa
    24061 aacaggttta acagatggca tgaccatttc tttccaccac catttccgtg aaggggattt
    24121 cattatgaat atggtcttgg aggaaattgc caaaatgggc attaaaaacc tgtctattgc
    24181 cccaagttcc atcgctaatg tacatgagcc tttgattgat cacatcaaaa atggtgtggt
    24241 cactaacatc acttcatctg gtcttcgtga caaagtaggg gcagccatct cagaaggact
    24301 aatggaaaat cctgtggtta ttcgctccca tggtggtcgc gctcgtgcca ttgctagtgg
    24361 ggatatccat attgatgttg cctttctggg cgccccaagt tcagatgctt acggaaatgt
    24421 taatgggaca aaagggaaag caacctgtgg ttctttgggc tatgccatga ttgatgccaa
    24481 atatgcggat caggtggtta tcttgacaga taatttggtt ccttatccta ataccccaat
    24541 cagcattcct caaacagatg ttgactatgt ggtaacagtg gatgctatcg gagatcctca
    24601 aggaatcgcc aaaggggcaa ctcgttttac gaaaaatcct aaagaactct taattgcaga
    24661 atacgccgct aaagtaatca ccaactctcc ttattttaaa gaaggattct ctttccaaac
    24721 tggaacaggt ggcgcttctt tggcagtaac ccgtttcatg cgtgaagcta tgattaaaga
    24781 aaatatcaaa gctagctttg ctcttggtgg tattaccaat gctatggtgg agttgctcga
    24841 agaaggactg gttgaaaaaa ttcttgatgt ccaagacttt gatcacccat cagctgtttc
    24901 tcttggtaag catgctgacc attatgaaat tgatgccaac atgtatgcct cacctttgag
    24961 caagggtgct gttatcaatc aattagacac ttgtatttta tcagcccttg aagtcgatac
    25021 taacttcaat gttaatgtga tgacaggatc tgacggcgtg attcgtggcg cttctggagg
    25081 acactgcgac actgcctttg cggctaagat gagtttggtt atttcaccac ttatccgcgg
    25141 acgcatccca acttttgtag atgaggttaa tacggtcatt acaccaggaa caagtgtcga
    25201 tgtggtcgtc acagaagtgg gaattgccat taacccaaat cgtcaagact tagtagacca
    25261 tttcaaatcc ctcaatgtgc cacaatttag tattgaggag ctaaaagaga aggcttacgc
    25321 tatcgttggc acacctgagc gtattcaata tggtgataag gttgttgctc ttattgaata
    25381 ccgtgatggt agcctcatgg atgtggttta caatgtgtaa agacacttat ttttcaggcg
    25441 aagctatcca acttagtgat atgttaagag cccgcgaaga aagagctctg cgtcagctgc
    25501 atttattaaa ggagtaccca gaaggtagct tattatcggt caccatgaat atccctggac
    25561 caattaaaac ctctcctaaa cttcttgaag tttttgatat agtgattaag gccattcaaa
    25621 ctgccttagc tgacgataag atttgttacc agttgcgatt actgcctaca acgggttatg
    25681 agtattacct catcacaagt ctacctagcc gcgacctgaa gttaaaaatg atagccttag
    25741 agacagagtt gccaataggt cgtctcatgg atttagatgt cttggtcttg caaaatgatc
    25801 tgcctcattc aattagcaga accgtattag gaggctcccc taggcaatgt tttatctgtt
    25861 ctaaagaggc caaagtctgc ggtcgcctac gtaagcacag tgtcgaggag atgcagactg
    25921 ctatttcaaa attactccat tcatttttca ataaagacaa ccaatcatcg tcatcagata
    25981 agacaggttg atgaggagaa caattatgac gaaaatccgt ataacggaaa cagttttacg
    26041 tgatggtcaa caaagtcaga ttgccacacg tatgacaacc aaggaaatga ttcctattct
    26101 cgaaacgcta gacaatgctg gttatcatgc ccttgaaatg tggggaggag caacctttga
    26161 ttcctgccta cgctttttaa atgaagaccc ttgggaacgg ctaagagcca tccgtaaagc
    26221 agtgaaaaag actaaacttc agatgctctt acgcggacaa aaccttcttg gatatcgcaa
    26281 ttatgctgac gatgtggtca gatccttcgt tcaaaaatcc attgaaaacg ggattgatat
    26341 tgtccgtatc ttcgatgctt taaatgaccc tcgcaacttg caaacagctg tttcagcgac
    26401 taaaaaattt ggagggcatg ctcaagttgc catttcttac acgacaagtc cggtacatac
    26461 cattgactac tttgttgaat tggcgaaagc ttaccaagct atcggagcgg actccatttg
    26521 tatcaaagac atggctggtg tcttaactcc tgaaataggt taccaattgg tcaaatgtat
    26581 caaggagaat acaactattc ctcttgaggt tcatacccat gctaccagtg gtatttcaga
    26641 aatgacttac ttaaaagtag cagaagcagg agctgatatt attgatacgg cgatttcctc
    26701 tttttcaggg ggaaccagtc agcctgccac agagtcaatg gcgattgcct tgacggattt
    26761 aggctttgac acaggcttgg atatgcaaga ggtggccaaa gttgcagaat atttcaacac
    26821 tattcgtgac cactatcgag aaatagggat tttaaatcct aaagtcaaag atactgagcc
    26881 aaaaacattg atttaccaag ttccaggtgg catgctatca aacctattga gccaattaac
    26941 cgagcaaggc ctagctgata aatacgaaga agtcttagca gaagtaccta aagtaagggc
    27001 tgatcttggg tatccgccac tcgtaacgcc attatcacaa atggttggaa cacaggcctt
    27061 gatgaatatc atctcagggg aacgctacaa ggtagttcca aatgaaatca aagactatgt
    27121 tcgagggcta tacggtcaat caccagcccc attagcagaa ggtatcaaag agaaaatcat
    27181 tggtgacgaa gcagtcattg cttgcagacc agctgactta atcgagcctc aaatgattta
    27241 tctacgtgac gagattgctc aatacgctcg ttcagaagaa gacgtgttaa gctatgcgag
    27301 tttcccacag caagccagag atttcttggg acgccgtgaa gatcctttct atgatgttcc
    27361 ggttcaagaa gttactgtac aactggacat tcaagactaa cagggaatga ctaatcctct
    27421 taaccattaa tctccttggt gaaacgcatt tgctgtaaga acaataccaa aaacctacct
    27481 gattttgata atcagatagg ttttttatct tagtaatgtt tgacagatga agtggcttgt
    27541 atctttttaa taatgggttg ggcttctagc gaacaaaaat aatctaaaga cgttttggga
    27601 aggagttttg ccagaaggtc ccaatcacta tctttcaatg cctgcctagc cgttgaggca
    27661 ctgataggat cttgctgctg attgatagct ttccttggca aaatatctag gaggatacca
    27721 gcttgctgca actgctctgc catgatttca ttgtaaatag cagttactcg gctggttggc
    27781 tcttctccaa cgtatctttt tgtaatctgt aaggtttgag caatctttag aaaaatagca
    27841 gtatccagca aagcttggct tttgatcaca tcattgtctt ccgtttggaa ataagctgga
    27901 aaggttgcct gactaatcaa ataaggacct gtttcatgaa agataatgtt atcaagatgt
    27961 ttcaatcctt cttgaatcaa catttttcgt actgaaaaag gaaccaagct ttggtcctca
    28021 ctgaccataa acagatgaac ccaatcattt tctcttgcag ctttctctac taaaaattga
    28081 tgccctaagg taaaggggtt agcgttgata acaatagcag cgactttacc aggtgtttgc
    28141 tcaggcttat taagtgtcat cagataatct tgaaaaccag tttttttatt ttccaagaaa
    28201 ctgatgtgat tgtcgatatg gacaatttca gtaaaaccaa gatctttgaa aaaagcagca
    28261 gaagaggtct tcgtataaac aaaaagatga tagttgccaa gggcatattc ttcatcaatg
    28321 agatgactca caatcctatt aagaaatcct ccaccttgat aggcactgga tacgcaaaga
    28381 cagcgcaagc tatttccaaa gtaagaaccg gtagcaatca catcattttg cgcattcatg
    28441 atggcacaag tataatctaa atgagcatcc aaacgaatac cttcttgatg aagcaggttc
    28501 tttacactag ccattgtcgt tttatctgaa ggaaagactt ttgaaatagt gtagtaaggc
    28561 atttcttctc cgtatcttta attttattga atcataacat aacttagtac caaaacaggc
    28621 tgatctatct aaaggcaact ctgtttttta tcaacgccat tttttaaaag gaaatcgaat
    28681 gtgtttgaca gactcaaaag tatcagcgat tttttgattg cgttccacag tccgcaagtt
    28741 ttcttggtct aataagtctt gacttttttc caaaacatca acagtaatgt agtagagttt
    28801 gatcaggctt cctatcttat atttaccaga ttttggagac atagcaacca cttcattgag
    28861 attactatga agccattttt tatgaggctt agcaggtata gtggctacta caaagccaag
    28921 ttgttccaaa tgagcttgtg cctgatcaac aggaagatta atcacatcat ctatcgtgcg
    28981 tagttgcatc tctttttcat gtttttgttc catacgtttt tcgataattg gtcgactgtt
    29041 atcaatcgtt tttccaataa tttcagtagt gtcaggaaca attgacatca gtttcccaac
    29101 tactcctaga gcttttctta ttctctttgg catcttttat ctcctcaagt cgtttctttt
    29161 tattataaca aaatgtctaa aaatagtatg cgataaacct tgttctaaaa ggttaaatag
    29221 atcaattcaa ggaaaaaata ggaggggagc cgacaaaaaa cacaaagatt ctaaaaaagt
    29281 caagctattt tagtttagcc tttgtcaatc gtatcagttc aaatagaaaa tacaaatttt
    29341 gacaagaatt tatttatgta acaaactgtg tttttgattc ttcagtctat ctaacgataa
    29401 tgttatctga ttcttgtttt caaccaattg atgttaaaga agctgtcttc atgattctct
    29461 aatattcctt caattttagc tttatattac tctaagttat cagtaatgac attaagtagt
    29521 ttaacaagaa taatcctaaa agctacttta caaaataacg tattatcata ctaaataaaa
    29581 gtactgacat actaaaaaca ggcttatctc atctttttga tgagaaccct actagattta
    29641 atctataaac atcacaaaaa agtcattcaa agaagggaca aagttagcag taatattcct
    29701 ttttctaagt ggcttgagta gtttggaaaa cggcctgaca ccaaactgtc acggttaata
    29761 gtagtcatct attaaagtta atttaaaagc tatttttatc tttaatgtta cataaaaaca
    29821 attatgtaac attaaaggtt atctaaagca tttttttgct catcattgac aatatgggta
    29881 taaagatccg tgacttgagt agaggaatgg cctaattgat gactaactaa cacctgagat
    29941 ttagtagcat catatagtct tgtcgctagc gtatgacgca atttgtgagg agtcacacga
    30001 attttaaaat cctcagagta cttgccaacc atcttttcta tactagaagc atccattcga
    30061 tttggaacgc cacggtattc tgttaaaaag aaagcaagat cctgtttttc agccttgtaa
    30121 cgtctctgac gaacagctaa ataagactcc aaataacctt tcgcaaaacc tgctacattg
    30181 actgaatcac gttttccgcc tttgcgaata acttcgataa tcatcatatt tagattgaca
    30241 tcttttaaat caagattcac tgcttcagac aaacgaacac cagaagcaag tagtagagca
    30301 ataatagcca aatccctctc cttattttta cggaaagaag atttggcacg attagacaat
    30361 ttttgctcat attccttatc cacataatcc aagaaagcta gagtttcatc acctaaaaac
    30421 agtttctgct taatattttc cgctctggag gccagggttt cttttttctt ttttgttgac
    30481 acttttttca taacattccg ataaaaataa ggctcccctt ggtcattttc aacctcttca
    30541 gtcaagtact tgtaaaggct tgataaggca gacaaggtcc gattaatagt ggtttgagaa
    30601 agcccctctt tggtggaata ggtgttgaga gaaggtcgtt cccgcaaata aagcacaaag
    30661 gcctctaaat ccttcttggt aaggtgctca agcgtgctca agtcaatatc agcaatcttt
    30721 ggcgcctgcg ttaaatcagc atccatcagc caatcaaaaa agcgtttgta ttcttttaaa
    30781 tactcatata aggtggtaaa gctgtatgga acagctaatt tggattggta ataatctaaa
    30841 acataccaag gcatgacagc cttgtaggtt tcaatttttt ctagtaataa ttcgcgtctc
    30901 atatcatcat aattccttaa caataacaat tttttcaaaa atgaattttc gcaaattgtc
    30961 tcatgtattt tatgccattc attctctaca agagcagtat atcacaccta taaatatttt
    31021 tcaagaaaat tacagttttt cggaaagatg tcacgagaca aatctctttt tttacagata
    31081 ctaacgtcac ttcttttaga ttcagtaata atttactatc attattttag taacttcagg
    31141 cacagaattt gtttagctca gaactaaatg ataattttat ctttaaagta attaactctt
    31201 tattagatat agacctgcta gtacacgaat ctagtcgcta tgttttaggt gtttcccctt
    31261 tttgacagta ttgctatcac atgaattaat tgtaggaaat gttacacttg ctttcgtttt
    31321 atttttttga tactattagt aaagttataa ctttactaaa ggagctacca aatgttttct
    31381 ggacatcaac taaaaacagc acgattatca aagggaataa ctcaatcaga attgggaaga
    31441 ttgttgcatg tcaataaaat gacaatatct aattgggaaa aaggtaagaa tataccaaat
    31501 gaaaaacatt taaatgcctt attgcatcta ttcaatgtga catctgatta tttcgaccca
    31561 aactatagat tgctaacgcc ttataaccag ctgacaatat ctaataaaga aaaagtaatt
    31621 ggctattcag agcgattgtt aaatcatcaa atagacaaaa aatctaaaga tctcatagat
    31681 aaaccatcac aattatatgc ttatcgggtc tatgaaagtt tatctgctgg tactggttac
    31741 tcctattttg gtgatggtaa ctttgatgtt gtcttttacg atgaacaatt agaatacgat
    31801 tttgcgtctt gggtttttgg agattctatg gagccaactt atttaaatgg cgaagttgtt
    31861 cttataaaac aaaatagttt tgattacgat ggagcaattt atgcagtcga atgggatggg
    31921 caaacatata tcaaaaaggt atttcgtgaa gatgagggat tacgtctagt gtccttaaat
    31981 aaaaaatatt ctgataagtt tgctccctat agcgaagaac ctcgcattat tggcaaaatt
    32041 atcgctaatt ttaggccctt agaaatttaa gttagattga ttaggttatt tcaaatttta
    32101 aatgaactca tcatgcatgt aacgcggtct tccataattt taataatctt gtatacaaga
    32161 taaatgaatt tcacatcaaa aagagacgaa aatttccgtc tcttttattt tcgttttttc
    32221 tttgcttttt ttagtttgtt agcttgacgt ttcatggcct gtttcatggc aaattgtcca
    32281 attttcccct tgaacccttt accaaatact tgactcatat ccatatcacc accaaggcca
    32341 gataaatcgg gcataccacc ttgtcccatc atcccttcta aggaagacat atctggcata
    32401 ccagcaggca tgtttttagg aagattattg ggattaatgc ccatatcttt catcatctta
    32461 gacatatcgc ccgacataac cccttgcatc atgcttttgg cttggttaaa gtctttgata
    32521 aatttattaa cttccacaaa gctgttcccg gaaccagctg caatacgacg gcgacgactc
    32581 gggtttagta agtcagggtt ttcacgttca gcgggggtca tcgaagagac aatagcacgt
    32641 ttgcgagcaa tttggttttc gtcaactttg atattggcca aagctgggtt accagccatt
    32701 cctggaatca ttttcaagag gtcttccata ggccccatat tttgcacttg atctaactgt
    32761 tcaatgaaat cattgaaatc aaaggtgttc tcacgcattt tttcagctaa ctctaaggat
    32821 tttttctcat catattcctg gctagctttt tcaataagag tgagtaagtc acccattccc
    32881 aaaatccgac tggacatgcg gtctgggtga aatgtttcaa tatctgtgat tttttcacca
    32941 atacctgtaa atttaattgg ttttccagtg atttcacgga cagatagtgc tgcacctcca
    33001 cgggtatcac catcaatctt tgttaacaca actcctgtga tgctcaactg atgattaaac
    33061 tcgtaggcta cattggcagc ttcttgacca atcatgctat ctacaaccaa aagaatttca
    33121 tttggttgtg ctaaagcctt gacatcgcgc agctcttcca tcagtttttc atcaatttgc
    33181 aagcgacccg cagtatcaat caagacatag tcattgcggt tttcacgagc ttgttccaaa
    33241 ccttttctga caatatcgac tgctgagtgg tctgtcccca tatcaaaaac aggtacattg
    33301 atttgttgcc ccagtgtttt taactgatca atggccgctg gacggtaaat atcggcagca
    33361 atcatcagtg gtcgcgcgtt ttcttcttta attaatttat tagcgagttt tcctgcaaaa
    33421 gtggttttac cagccccttg caaaccgacc atcatgataa tagttggaat ttttggagat
    33481 ttatcaatct ccgcggtttc tgagcctaaa atactcgtta attcttcatt aacaatctta
    33541 agaatttgtt gtgttggatc aagagtatca ataatctcat ggccaatagc gcgctcacgt
    33601 acgcgcttaa taaaggtttt aacaactggt aaagcgacat cggcttctaa aagagctaga
    33661 cgaatctctt ttgtcacttc ttgaacatct gattctgata atttcttttt accgcgaatg
    33721 tgtttaaaca catcttgcaa acgttgggtt aagctttcaa aagccatagt caaaactcct
    33781 cttactctct attgtcaata ctggttagaa tggaaatttt ttcttgcaaa tactcatcat
    33841 gagggtagtg agcaatcatg tcgtcaaaga tttcacttcg aacaacataa tccgaataca
    33901 tatgaagttt catctcataa gtctctaaaa ttttttccgt acgcttaata ttatcataga
    33961 cagcctgacg actgacacca aattcatcag caatctcagc taagctataa tcatcggcat
    34021 aatacagctc aatataattc atttgtttat ctgttagcaa agctgcataa aattcaaaaa
    34081 gggcattcat cctgtttgtt ttttcaattt ccataatgtt cattttatca tactatgatt
    34141 gtttttaaca gtagcaaatt aattgcctta tgctgttcgt gtaaaaaagg aagtcataaa
    34201 tgacttcttt actaattatt ttctaagtaa aattcaaaac ggtcaccgat atactgactg
    34261 cggacatact caaaaggttt gccgtctgta aagtaagaca cctgagttag agccaaaatg
    34321 gcatgtcctt tagcaacttc caagtaggag gctacacgct cacttgctag cttagcgtag
    34381 attgtctgtt tacttttacc aatctcgtaa ccatttgcta tcaatgagtg aaaaaagtgt
    34441 tctgtaatat cagctcgttt aaccgttttt ataaattttt ctgggattga agcaatttcg
    34501 tacaccaaag ggacactgtc tgcatagcgg atacgttcca ttcgaatgac caagtctgtt
    34561 ttgtctaagt tcaattcttt aacttcggta tcactagcta attgcttttg ataggataat
    34621 aatttagagg agggttgtcg tccttgggag cgtataattt ctgtgaaact tgttgttcct
    34681 cgcatttttt cttgaacacg atgactggca acataagtac cacttcctat ccgcctttca
    34741 agaatccctt cttctactag taatgtaata gcctgccgta gagtcattcg actaacggtg
    34801 aaatgttccg ctaaatgtcg ttcacttggt aagcgactac caattggcca gatgcccaga
    34861 tcaatgtctt ttttaattgc atcatgaatt tttatataag ctggtaacat gccacactcc
    34921 tcaagtcggt tattcatcta ttttatcata tttttgatga aaattgaatt gtaaatattg
    34981 aggtatttgg catcaattaa tgatattacc tgcgacaata actggtgaat ttggtggaaa
    35041 atgaataggt tccataaacc aactagtgag cttcgcttta gtaacaatgg atgaggtaaa
    35101 aacattattt aaaaccctaa taactcctac ttttgaggaa tgctgaccaa tagaaccact
    35161 ataggctctc aggcaaccag tcggatctat ataaggtaaa aattgatttt ggcttacagt
    35221 aatattgtga catagatgtc gagtttggtt aaatgcttca tagtttggag caatattact
    35281 aagtagacct ccatcccatg caaaatgatg aacagcagca tcaaactgaa tgacttctga
    35341 aaaaaaatca tgcccttgag cctttgatag cagctgttgg tcttgtacta attctggcgc
    35401 atagccaata aattggtttt tctcaaacaa tgaattttgt agagatccta aatcaaaaat
    35461 atgactatta cgcttatgaa ccatagtaaa tttgttatcg taaatatgcc aatcagtacc
    35521 atgatcagcc ataatcataa agtggtctcc ttttttcaaa tcatttgctt tgaaatgcac
    35581 tccagtcaac acgagattac gaacaccttg ttcagccttt ggtcctgtag ggaatccaaa
    35641 ccacaacatt ttaccgtgaa taatgaactc tgtctgatga ccaataatag ctgtatcaga
    35701 tggaagctca aaataatctt tttgtgaagg caaagagcca atgtgaaatt gcccctttgg
    35761 aaaagatata acgctatttg gatgctgctt tgcataagcg aaagcttttc gcaaaagacg
    35821 attgttttgc tcaggggaat ttcctttatt gaacctaata taataaggtt taacaaaatc
    35881 ttgctctgaa attccatcta atcttagatt atctttcagg ttaatatcgt actgatatcc
    35941 tcctaacgaa tagtcatagc gttttttaac ttgtttaata gtcgctaatt gaccttgata
    36001 cacttttata tgtggctttt tagttaatga agataattta acgatatctc cttttttaaa
    36061 cctcggttta tcaggctctt tcaggttatg ttctggcaaa gaaacagtga cttttcctat
    36121 tggaaatacc acttcataat gataactcac tcggtaattt tcggctatat cttctacctg
    36181 tttattaata gttccaatcc attgatgata tggaccactt ttttgtactt gaatagcttg
    36241 tccaatgtga tagcgaggct gttgatcttt gcttcgttcc actattccga cagttgctaa
    36301 tatattgtga agtgttgcaa aactattggt ccacgcaagg tacgaaacaa ctaaactcaa
    36361 caataatcct aggaaaatta attcaacagg tcgaaataaa cccaaaattc tcttttttct
    36421 tagcataggc cctccctgtt gtcttaattc cctttcatag cctctattgt aacataacga
    36481 ttacgttttt ttccaacgag ctagagaaat tgtaatgtct tttcgatgaa aatatgctac
    36541 aataagttga caactatcag cctgacggtt ggtatagaat gaaaggatta taatgactga
    36601 aatttcaatt ttgaatgatg ttcaaaaaat tatcgttctt gattatggta gccagtacaa
    36661 tcagcttatt gctagacgta ttcgagagtt tggtgttttc tccgaactaa aaagccataa
    36721 aatcaccgct caagaacttc gtgagatcaa tcccataggt atcgttttat caggagggcc
    36781 taactctgtt tacgctgata acgcctttgg cattgaccct gaaatctttg aactagggat
    36841 tccgattctt ggtatctgtt acggtatgca attaatcacc cataaattag gtggtaaagt
    36901 tgttcctgct ggacaagctg gtaatcgtga atacggtcag tcaacccttc atcttcgtga
    36961 aacgtcaaaa ttattttcag gcacacctca agaacaactc gttttgatga gccatggtga
    37021 tgctgttact gaaattccag aaggtttcca ccttgttgga gactcaaatg actgtcccta
    37081 tgcagctatt gaaaatactg agaaaaacct ttacggtatt cagttccacc cagaagtgag
    37141 acactctgtt tatggaaatg acattcttaa aaactttgct atatcaattt gtggcgcgcg
    37201 tggtgattgg tcaatggata attttattga catggaaatt gctaaaattc gtgaaactgt
    37261 aggcgatcgt aaagttcttc taggtctttc tggtggagtt gattcttcag ttgttggtgt
    37321 tctacttcaa aaagctatcg gtgaccaatt aacttgtatt ttcgttgatc acggtcttct
    37381 tcgtaaagac gagggcgatc aagttatggg aatgcttggg ggcaaatttg gcctaaatat
    37441 tatccgtgtg gatgcttcaa aacgtttctt agaccttctt gcagacgttg aagatcctga
    37501 gaaaaaacgt aaaattattg gtaatgaatt tgtctatgtt tttgatgatg aagccagcaa
    37561 attaaaaggt gttgacttcc ttgcccaagg aacactttat actgatatca ttgagtcagg
    37621 aacagaaact gctcaaacca tcaaatcaca tcacaatgtg ggtggtctcc ccgaagacat
    37681 gcagtttgaa ttgattgagc ccttaaacac tcttttcaaa gatgaagttc gagcgcttgg
    37741 aatcgctctt ggaatgcctg aagaaattgt ttggcgccaa ccatttccag gtcctggact
    37801 tgctatccgt gtcatgggag caattactga agaaaaactt gaaaccgttc gcgaatcaga
    37861 cgctatcctt cgtgaagaaa ttgctaaggc tggacttgat cgtgacgtgt ggcaatactt
    37921 tacagttaac acaggtgtcc gttctgtagg cgtcatggga gatggtcgta cttatgatta
    37981 taccatcgcc attcgtgcta ttacgtctat tgatggtatg acagctgact ttgctcaact
    38041 tccttgggat gtcttgaaaa aaatctcaac acgtatcgta aatgaagttg accacgttaa
    38101 ccgtatcgtc tacgacatca caagtaaacc acccgcaaca gttgaatggg aataacagaa
    38161 taaacattaa aagactcgct cattttggcg agtcttttaa tgtttattct ttaactgttt
    38221 tcgtattttg tacatggtat tagcaatatg ataaagtggg ctgacaggga gattaaattc
    38281 tcctataaat tcttctattg tcggagcaaa gttggcctta aatttggtaa gtccatcatc
    38341 aagcgagcct tctactcctc ccatgttagc ccaaataata ccatcttgat aagcatcctg
    38401 aaaaacttta ggatacagca gatactgagg ataaaacttc ttaaaatcat cattcatccc
    38461 agcataaagc atttccataa catttccata agaaatagat aatatacctg ctacaacaac
    38521 ttcctcagga tattggtctg taaagccttc aaattcagtt atataacgtt caaaagaagc
    38581 tttttgatct tctaattttt ttaaacgctt cttttgatga gcttgggtgc gagtaatatc
    38641 ttggttaata agatttaatt gctggcggta ttgatctagt ttttgaggaa tattcacttt
    38701 tgctagatgt aagtaggcct tatccccata agtagtcatc aacttttgaa agtaagcttc
    38761 gttacgcaaa gaaatatttt tacgtttttc tgttaaggag acaatcttgg aaaatttgtg
    38821 aagttctgat tgactggcac gatatgtttc tacaccacgc tgcttagcat cttttattaa
    38881 acgtctggta tgcttaggaa attgcatctc aaggttttct tgagtgtaaa tattagcttg
    38941 gaaacgaggt tggatactat ctgcaatctc cattgttaaa ccagtccaat gaacaccagc
    39001 ttcttggaga ttcttaatag ctgctaaagc taaaggtttt tcttcttctt cctgtcccag
    39061 tgcgtattgt tttaacagga tagctggatc atatttgata aagagggcct ttttagtttt
    39121 accataatcc tttagtgttt tcatggtaaa tgccaccaaa tcacggtcaa gataatccat
    39181 gactggccct cttggaatat aaataatgct tcttccaagt ggcaatagct taatcaaaag
    39241 tgataaagag gcaacctgct tttcctcttt atagatacca attcgctcat tctgccattg
    39301 atttttgatt tttgcccaat cactaccttg taaaacgcta atttgctgat gttctttaac
    39361 aaaagagtca tgctcttctt ctgaaatacc gatttttgca taaaacgtta aattcgtcat
    39421 ttattgtccc catctattcg taatcttagc agatgtactt actaagtatc tt
//
LOCUS       DATXQK010000014        33070 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.014, whole genome shotgun sequence.
ACCESSION   DATXQK010000014 DATXQK010000000
VERSION     DATXQK010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33070
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.014"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            3..74
                     /locus_tag="VC394_000758"
     tRNA            3..74
                     /locus_tag="VC394_000758"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36..38,aa:Glu,seq:ttc)
     gene            85..174
                     /locus_tag="VC394_000759"
     tRNA            85..174
                     /locus_tag="VC394_000759"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Ser,seq:tga)
     gene            184..257
                     /locus_tag="VC394_000760"
     tRNA            184..257
                     /locus_tag="VC394_000760"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:218..220,aa:Met,seq:cat)
     gene            278..350
                     /locus_tag="VC394_000761"
     tRNA            278..350
                     /locus_tag="VC394_000761"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:311..313,aa:Phe,seq:gaa)
     gene            370..450
                     /locus_tag="VC394_000762"
     tRNA            370..450
                     /locus_tag="VC394_000762"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:404..406,aa:Tyr,seq:gta)
     gene            457..527
                     /locus_tag="VC394_000763"
     tRNA            457..527
                     /locus_tag="VC394_000763"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:489..491,aa:Trp,seq:cca)
     gene            539..611
                     /locus_tag="VC394_000764"
     tRNA            539..611
                     /locus_tag="VC394_000764"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:572..574,aa:His,seq:gtg)
     gene            620..691
                     /locus_tag="VC394_000765"
     tRNA            620..691
                     /locus_tag="VC394_000765"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Gln,seq:ttg)
     gene            711..794
                     /locus_tag="VC394_000766"
     tRNA            711..794
                     /locus_tag="VC394_000766"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:745..747,aa:Leu,seq:caa)
     gene            1054..1239
                     /locus_tag="VC394_000767"
     CDS             1054..1239
                     /locus_tag="VC394_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994247.1"
                     /translation="MSFEIFTLIIGWKLVGYRVMECGSSFKQGDYRLVVHICLLNVRD
                     EMLSQQGHEDKADWPEP"
     gene            1877..2185
                     /locus_tag="VC394_000768"
     CDS             1877..2185
                     /locus_tag="VC394_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-diphosphocytidyl-2C-methyl-D-erythritol
                     kinase"
                     /protein_id="HEP5994248.1"
                     /translation="MKHRFQVKRGVSVYLEKRIPMCAGMGGGSSDAVTIRALNQLWLL
                     TLSRKDMMDIGIPIGSDVPYCLLSGRAQVTGKGEVVCRILGLLSSWVVLVKPDFGIST
                     "
     gene            2240..2452
                     /locus_tag="VC394_000769"
     CDS             2240..2452
                     /locus_tag="VC394_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-diphosphocytidyl-2C-methyl-D-erythritol
                     kinase"
                     /protein_id="HEP5994249.1"
                     /translation="MVAAIEAGNYNDGILTEMNNLLEDIFIAKRPFIQKIKEKTLQAG
                     AATTLMTGSGPTVFALCQTEKQTIIV"
     gene            2562..3005
                     /locus_tag="VC394_000770"
     CDS             2562..3005
                     /locus_tag="VC394_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent MarR family transcriptional
                     regulator"
                     /protein_id="HEP5994250.1"
                     /translation="MGTLEKKLDNLVNTILLKAENQHELLFGACQSDVKLTNTQEHIL
                     MLLSQQRLTNTDLAKALNISQAAVTKAIKSLVKQDMLAGTKDTVDARVTYFELTELAK
                     PIASEHTHHHDETLNVYNRLLQKFSAKELEIVDKFVTVFAEELEG"
     gene            3009..3728
                     /locus_tag="VC394_000771"
     CDS             3009..3728
                     /locus_tag="VC394_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEP5994251.1"
                     /translation="MRYISVKNLSFQYESEPVLEGITYHLDSGEFVTMTGENGAAKST
                     LIKATLGILQPKAGRVTIAKKNKDGKQLRIAYLPQQVASFNAGFPSTVYEFVKSGRYP
                     RSGWFRHLNKHDEEHVQASLEAVGMWENRHKRIGSLSGGQKQRVVIARMFASDPDIFV
                     LDEPTTGMDSGTTDTFYELMHHSAHQHGKSVLMITHDPEEVKAYADRNIHLVRNQKLP
                     WRCFNIHEVETDDEKGGHGHA"
     gene            3721..4536
                     /locus_tag="VC394_000772"
     CDS             3721..4536
                     /locus_tag="VC394_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEP5994252.1"
                     /translation="MLDILSYDFMQRAVMAVVAISIFAPILGIFLILRRQSLMSDTLS
                     HVSLAGVALGVVLGISPTITTIIVVVLAAILLEYLRVVYKHYMEISTAILMSLGLALS
                     LIIMSKSHSSSSMSLEQYLFGSIITISMEQVVALFAIAAIILILTVLFIRPMYILTFD
                     EDTAFVDGLPVRLMSVLFNIVTGIAIALTIPAAGALLVSTIMVLPASIAMRLGKNFKT
                     VILLGIVIGFSGMLSGIFLSYFFETPASATITMIFISIFLLVSLGGMLKKRLF"
     gene            complement(4576..4959)
                     /locus_tag="VC394_000773"
     CDS             complement(4576..4959)
                     /locus_tag="VC394_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEP5994253.1"
                     /translation="MTECIFCHQLKANQLLTQSQYFKVVFDIDPIQKGHLLLISKDHY
                     TSLTQLPKEVRYDLIDLQAALVAKLEQHLPISGVTSVSNDKELMDEGTHFHLHLIPRL
                     TNDSFWESITINQENWDLTPFLKHL"
     gene            complement(5010..6266)
                     /locus_tag="VC394_000774"
     CDS             complement(5010..6266)
                     /locus_tag="VC394_000774"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEP5994254.1"
                     /translation="MNIFEELKARGLVFQTTDEQALVKALTEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKETVLEWSDKIK
                     GQLSTFLDFENGDNKAELVNNYDWFSQISFIDFLRDVGKYFTVNYMMSKDSVKKRIET
                     GISYTEFAYQIMQGYDFYELNDKHNVTLQIGGSDQWGNMTAGTELLRKKADKTGHVMT
                     VPLITDSTGKKFGKSEGNAVWLDADKTSPYEMYQFWLNVMDDDAVRFLKIFTFLFLDE
                     IAEIETQFNAARHERLAQKTLAREVVTLVHGEEAYKQALNITEQLFAGNIKNLSANEL
                     KQGLSNVPNYHVQSEDSLNLVDMLVTAGISPSKRQAREDVQNGAIYINGDRVQDLDYQ
                     LSNDDKIDDQLTVIRRGKKKYAVLTY"
     gene            6358..8670
                     /locus_tag="VC394_000775"
     CDS             6358..8670
                     /locus_tag="VC394_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEP5994255.1"
                     /translation="MRYFMVKWNTKQKRISHQRLGLLDLGPVLLRTLRLLSNFFYIVI
                     FLFGMMGFGMAFGYLASQIESVKVPSKESLVKQVESLTMISQMNYSDNSLISTLDTDL
                     LRTPVANDAISENIKKAIVSTEDEHFQEHKGVVPKAVFRATLASVLGFGEASGGSTLT
                     QQLVKQQVLGDDPTFKRKSKEIVYALALERYMSKDNILCDYLNVSPFGRNNKGQNIAG
                     VEEAARGIFGVSAKDLTVPQAAFLAGLPQSPIVYSPYLSTGQLKSEKDMAYGIKRQQN
                     VLYNMYRTGVLSKKEYEDYKAYPIQKDFIQPGSAIVNNHDYLYYTVLADAKKAMYSYL
                     IKRDKVSSRDLKNDETKAAYEERALTELQQGGYTITTTINKPIYNAMQTAAAQFGGLL
                     DDGTGTVQMGNVLTDNATGAVLGFVGGRDYALNQNNHAFNTIRSPGSSIKPIIAYGPA
                     IDQGLMGSASVLSNYPTTYSSGQKIMHADSEGTAMMPLQEALNTSWNIPAFWTQKLLR
                     EKGVDVENYMTKMGYKIADYSIESLPLGGGIEVSVAQQTNAYQMLSNNGLYQKQYIVD
                     KITASDGTVVYKHENKPIRIFSAATATILQELLRGPITSGATTTFKNRLAAINPWLAN
                     ADWIGKTGTTENYTDVWLVLSTPKVTLGGWAGHDDNTSLAPLTGYNNNSNYLAYLANA
                     INQADSNVIGVGQRFNLDPGVIKANVLKSTGLQPGTVNVNGHTFSVGGEMTTSLWSQK
                     GPGAMTYRFAIGGTDADYQKAWGNFGFRKN"
     gene            8934..12500
                     /gene="rpoB"
                     /locus_tag="VC394_000776"
     CDS             8934..12500
                     /gene="rpoB"
                     /locus_tag="VC394_000776"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEP5994256.1"
                     /translation="MAGHEVRYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFQDFLDSG
                     LKEVFEDVLPISNFTDTMELEFVGYEFKEPKYTLEEARIHDASYSAPIFVTFRLVNKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELETDSKDIAYTRIDRTRKIPFTTLVRALGFSGDDEIVDIFGESDLVR
                     NTIEKDIHKNPSDSRTDEALKEIYERLRPGEPKTADSSRSLLIARFFDARRYDLAAVG
                     RYKVNKKLNIKTRLLNQIIAENLVDAETGEILVEAGTEMTRSVIESIEEHLDGDLNKF
                     VYTPNDYAVVTEPVVLQKFKVVSPIDPDRVVTIVGNANPDDKVRALTPADILAEMSYF
                     LNLAEGLGKVDDIDHLGNRRIRAVGELLANQFRIGLARMERNVRERMSVQDNDVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSFGHLNKYGFIQTPYRKVDRATGRVTNEIV
                     WLTADEEDEYTVAQANSKLNEDGTFAEEIVMGRHQGNNQEFSASVVDFVDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLIDPKAPYVGTGMEYQAAHDSGAAVIAQH
                     NGKVVFSDAEKVEIRRQDGSLDVYHITKFRRSNSGTAYNQRTLVKVGDIVEKGDFIAD
                     GPSMENGEMALGQNPVVAYMTWEGYNFEDAVIMSERLVKEDVYTSVHLEEFESETRDT
                     KLGPEEITREIPNVGEEALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGGDGIVRDVKIFTRANGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARNLGIHIATPVFDGASSEDLWDTVREAGMDSDAKTVLYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDVTGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDN
                     EVELRDLDEGEDDDIMHVDDLEKAREKQAQETQEVSETTDEK"
     gene            12591..16232
                     /gene="rpoC"
                     /locus_tag="VC394_000777"
     CDS             12591..16232
                     /gene="rpoC"
                     /locus_tag="VC394_000777"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020904830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEP5994257.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRAKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEPKSLLTEREYREKLQ
                     EYGHGSFVAKMGAEAIQDLLKRVDLAAEIAELKEELKSASGQKRIKAVRRLDVLDAFN
                     KSGNKPEWMVLNILPVIPPDLRPMVQLDGGRFAASDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVAKEYAGNVKAAKRMVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARLLMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEDA
                     GREGEGMIFKDKDEAVMAYRNGYAHLHSRVGIAVDSMPNKPWKDSQRHKIMVTTVGKI
                     LFNDIMPEDLPYLQEPNNANLTEGTPDKYFLEPGQDIQEVIDRLDINVPFKKKNLGNI
                     IAETFKRFRTTETSAFLDRLKDLGYYHSTLAGLTVGIADIPVIDNKAEIIDAAHHRVE
                     EINKAFRRGLMTDDDRYVAVTTTWREAKEALEKRLIETQDPKNPIVMMMDSGARGNIS
                     NFSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADS
                     GYLTRRLVDVAQDVIIREDDCGTDRGLLIRAITDGKEVTETLEERLQGRYTRKSVKHP
                     ETGEVLIGADQLITEDMARKIVDAGVEEVTIRSVFTCATRHGVCRHCYGINLATGDAV
                     EVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRIQEIFEARNPKGEAV
                     ITEVKGNVVEIEEDASTRTKKVYVQGKTGMGEYVVPFTARMKVEVGDEVNRGAALTEG
                     SIQPKRLLEVRDTLSVETYLLAEVQKVYRSQGVEIGDKHVEVMVRQMLRKVRVMDPGD
                     TDLLPGTLMDISDFTDANKDIVISGGIPATSRPVLMGITKASLETNSFLSAASFQETT
                     RVLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNIEPQAMNEIEVIDHTEVSA
                     EAVFTAEAE"
     gene            16384..16749
                     /locus_tag="VC394_000778"
     CDS             16384..16749
                     /locus_tag="VC394_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEP5994258.1"
                     /translation="MYQVIKMYGDWEPWWFIDGWQDDIIDEQQFSDWQEALDYFNQEW
                     QRMKAIFPSYHSQKNLLATFWEKEDKRWCEDCDEDLQQFHSLLLLKNKDIVPSNNYIP
                     EFEQRNDSPQVAYLCKLNL"
     gene            16842..17780
                     /gene="tadA"
                     /locus_tag="VC394_000779"
     CDS             16842..17780
                     /gene="tadA"
                     /locus_tag="VC394_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HEP5994259.1"
                     /translation="MVQALAKAILAKAEQVQAQDIYILPRADQYDLFLRIGDERRLVD
                     VYQSDRMAPLISHFKFVAGMIVGEKRRCQVGSCDYKLSKDKQLSLRLSSVGDYRGQES
                     LVIRLLHHQNKSVHYWFDGLTKVANQVGGRGLYLFAGPVGSGKTTLMYQLISSYHQEA
                     QVISIEDPVEIKNHQILQLQVNDDIGMTYDNLIKLSLRHRPDILVIGEIRDSQTARAV
                     IRASLTGAMVFSTVHAKSISGVYVRLLELGVTKAELSNCLALIAYQRLLNGGALIDST
                     QNEFEYYSSSNWNQQIDQLLEAGHLNPKQAKLEKII"
     gene            17764..18750
                     /locus_tag="VC394_000780"
     CDS             17764..18750
                     /locus_tag="VC394_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEP5994260.1"
                     /translation="MKKLSSKHQHKFIQLLANLLSTGFSFAEVIAFLKRSQLLQLDYV
                     LKMEESLLKGQDLADMLSGLGFSDAILTQISLADRHGNIETTLVAIQHYLNQMARIRR
                     KTVEVITYPLILLLFLFVMMLGLRRYLVPQLETQNQITYFLNHFPAFFIGFCSGLILL
                     FGMVWLRWRSQSRLKLYSRLSRYPFLGKLLKQYLTSYYAREWGTLIGQGLDLMTILDI
                     MAIEKSSLMKELAEDIRMSLLEGQAFHIKVATYPFFKKELSLMIEYGEIKSKLGAELE
                     IYAQESWEQFFSQLYQVTQLIQPAIFLVVAVTIVMIYAAILLPIYQNMGGIF"
     gene            18752..19078
                     /locus_tag="VC394_000781"
     CDS             18752..19078
                     /locus_tag="VC394_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEP5994261.1"
                     /translation="MINQWNNLRHKKLKGFTLLEMLLVILVISVLMLLFVPNLSKQKD
                     RVTETGNAAVVKLVENQAELYELSQGSKPSLSQLKADGSITEKQEKAYQDYYDKHKNE
                     KARLSN"
     gene            19053..19481
                     /locus_tag="VC394_000782"
     CDS             19053..19481
                     /locus_tag="VC394_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEP5994262.1"
                     /translation="MKKPVLAIKAFTLLETLLSLSVMSFIILGLSVPVTKSYQKVEEH
                     LFFSHFEHLYRHQQKLAILQQKQRVLDISSTKIVTEGNSLTVPKSITVNHPYRLVIDQ
                     MGGNHSLAKIIFDMTDRRFKYQFYLGSGNYQKTSQSLHSP"
     gene            19486..19722
                     /locus_tag="VC394_000783"
     CDS             19486..19722
                     /locus_tag="VC394_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComG"
                     /protein_id="HEP5994263.1"
                     /translation="MIATGILFSIVILVLSSLQQSQAALTYYRKQQEKLNLALMAVQT
                     RTKEITLNGCHITILRTDRYISIHDDEGEVMKIE"
     gene            19715..20149
                     /locus_tag="VC394_000784"
     CDS             19715..20149
                     /locus_tag="VC394_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEP5994264.1"
                     /translation="MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYL
                     ARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEKGKKVLAFGQFKSHDFRKSASNG
                     KGYQPMLFGISRSHIHIEQSQICITLKWKSGLERTFYYAFQD"
     gene            20133..20459
                     /locus_tag="VC394_000785"
     CDS             20133..20459
                     /locus_tag="VC394_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComG"
                     /protein_id="HEP5994265.1"
                     /translation="MPFKTNLKAGILLYAMFMASIFLLVLQVYLSQVTALHKEYQAQT
                     DYVKARLIAEIVYQDHRYKASNPVFFKGGQVICRERKERWMLIVKLDQQRQYQFEYLK
                     PKQYSQ"
     gene            20557..21510
                     /locus_tag="VC394_000786"
     CDS             20557..21510
                     /locus_tag="VC394_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEP5994266.1"
                     /translation="MTFEKIEEAYQLLLENCQLIENDLKTHIYDAIVEQNSFYLGAEG
                     ASPQVAQNSDKLKALCLTKEEWRKAYQFLFIKAAQTEQLQANHQFTPDAIGFILLYLL
                     EQLSDKDSLEVLEIGSGTGNLAQTLLNNTSKSLDYVGIELDDLLIDLSASIAEIMDSS
                     AHFIQEDAVRPQLLKESDIVISDLPVGYYPNDDIAKRYKVASSDKHTYAHHLLMEQSL
                     KYLKKDGFAIFLAPVNLLTSPQSQLLKQWLKDYAQVVTLITLPDSIFGHPSNAKSIIV
                     LQKQTDHPMETFVYPIRDLKLAENIHDFMENFKKWKLSNVN"
     gene            21569..22765
                     /locus_tag="VC394_000787"
     CDS             21569..22765
                     /locus_tag="VC394_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEP5994267.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEEEVLAQGIIERIGLKDSISTV
                     KYDGKKEEQILDIHDHTEAVKILLNDLIHFGIIAAYDEITGVGHRVVAGGELFKESVV
                     VNDKVLEQIEELSVLAPLHNPGAAAGIRAFRDILPDITSVCVFDTSFHTSMAKHTYLY
                     PIPQKYYTDYKVRKYGAHGTSHKYVAQEAAKMLGRPLEELKLITAHIGNGVSITANYH
                     GKSVDTSMGFTPLAGPMMGTRSGDIDPAIIPYLIEQDPELKDAADVVNMLNKKSGLSG
                     VSGISSDMRDIEAGLQEDNPDAVLAYNIFIDRIKKCIGQYFAVLNGADALVFTAGMGE
                     NAPLMRQDVIGGLTWFGMDIDPEKNVFGYRGEISTPESKVKVLVISTDEELCIARDVE
                     RLKNTK"
     gene            22952..23260
                     /locus_tag="VC394_000788"
     CDS             22952..23260
                     /locus_tag="VC394_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994268.1"
                     /translation="MYVFIEPNDLKDYLITVYDERNRAIRKQTQVYCFYLMIVLFVAL
                     FFFFPCLTIRDLVSYKLALIFAYLYLIIRSLVAKKMEALGKITTNSQKPPFDTSQVLC
                     "
     gene            complement(23343..24113)
                     /locus_tag="VC394_000789"
     CDS             complement(23343..24113)
                     /locus_tag="VC394_000789"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEP5994269.1"
                     /translation="MKIGIIGVGKMASAIIKGLKQTPHELIISGSSLERSKEIAEQLT
                     LPYAMSHQDLIDQVDLVILGIKPQLFETVLKPLHFKQPIISMAAGISLQRLATFVGQD
                     LPLLRIMPNMNAQILQSSTALTGNALVSQELQARVRNLTDSFGSTFDISEKDFDTFTA
                     LAGSSPAYIYLFIEALAKAGVKNGMPKAKALEIVTQTVLASASNLKTSSQSPHDFIDA
                     ICSPGGTTIAGLMELERLGLTTTVSSAIDKTIDKAKSL"
     gene            complement(24161..25228)
                     /gene="pepA"
                     /locus_tag="VC394_000790"
     CDS             complement(24161..25228)
                     /gene="pepA"
                     /locus_tag="VC394_000790"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEP5994270.1"
                     /translation="MTDLFSKIKEVTELDGIAGYEHSVRDYLRTKITPLVDRVETDGL
                     GGIFGIRDSKAEKAPRILVAAHMDEVGFMVSDIKVDGTLRVVGIGGWNPLVVSSQRFT
                     LYTRTGQVIPLISGSVPPHFLRGANGSASLPNIENIVFDGGFTDKAEAESFGITPGDI
                     IVPQSETILTANQKNIISKAWDNRYGVLMITEMLEALKGQDLNNTLIAGANVQEEVGL
                     RGAHVSTTKFDPELFFAVDCSPAGDIYGNPGTIGDGTLLRFYDPGHVMLKDMRDFLLT
                     TAEDAGVNFQYYCGKGGTDAGAAHLQNGGVPSTTIGVCARYIHSHQTLYAMDDFVEAQ
                     AFLQAIIKKLDRSTVDLIKRY"
     gene            25684..25977
                     /locus_tag="VC394_000791"
     CDS             25684..25977
                     /locus_tag="VC394_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEP5994271.1"
                     /translation="MRDMSKKKIGMISGIFGFSLAIGLGIVIKDYCQDRQRRQMTRDL
                     RTFFSPLGQIEVLYINPCQVKQDYISGGVVMSNGKQYQFTYHSRQISFEEDKG"
     gene            25974..26291
                     /locus_tag="VC394_000792"
     CDS             25974..26291
                     /locus_tag="VC394_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEP5994272.1"
                     /translation="MIRPTSYESLAALIEKEDKLVLFFTADWCPDCQFIYPIMPEIEA
                     ELTDMTFICVNRDQFIEVAQKWNIFGIPSFVVIEKGQEVGRLVNKMRKTKTEIMHFLA
                     AYQ"
     gene            26309..26935
                     /locus_tag="VC394_000793"
     CDS             26309..26935
                     /locus_tag="VC394_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEP5994273.1"
                     /translation="MIFAYNKEQVGDVLMVILQDTKDIKRQVERKGKVARVFAEESGK
                     TLAWNIFEVSSLITIEGNGQIFLTDEDLARLNAELAKEGFSERLEPTVGPVFVVGQIV
                     EMVAHPDSDHLNICQVAIGEDQTVQIVAGAPNAALGLKTIVALPGAIMPNGSLIFPGK
                     LRGEESYGMMCSPRELVLPNAPQKRGIIELDESAVVGEAFDPAKHWKG"
     gene            27087..27482
                     /locus_tag="VC394_000794"
     CDS             27087..27482
                     /locus_tag="VC394_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEP5994274.1"
                     /translation="MYNKVIAIGRLVAKPELVKTATDKHVARLSLAVNRRFKNASGER
                     EADFISIVVWGKLAETLVSYASKGSLMSIDGELRTRKYDKDGQVHYVTEVLCQSFQLL
                     ESRAQRAMRENNVTNDLVDLVLEEDTLPF"
     gene            complement(27743..28384)
                     /locus_tag="VC394_000795"
     CDS             complement(27743..28384)
                     /locus_tag="VC394_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEP5994275.1"
                     /translation="MLIVLAGTIGAGKSSLAAALGEHLGTDVFYEAVDNNPVLDLYYQ
                     DPKKYAFLLQIYFLNKRFKSIKEAYQADNNILDRSIFEDELFLKLNYKNGNVTKTELD
                     IYQELLANMLEELEGMPKKRPDLLIYIDVSFDKMLERIEKRGRSFEQVDGNPSLEQYY
                     HQVHGEYPTWYEDYKVSPKMKIDGNSLDFVQNPQDLATVLKMIDTKLKELHLL"
     gene            complement(28404..29381)
                     /gene="dusB"
                     /locus_tag="VC394_000796"
     CDS             complement(28404..29381)
                     /gene="dusB"
                     /locus_tag="VC394_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEP5994276.1"
                     /translation="MTKLNSSFRIGDVEIPHRTVLAPMAGVTNSAFRTIAKEFGAGLV
                     VMEMISEKGLLYNNEKTLHMLHIDENEHPMSIQLFGGDAEGLKRAADFIQTNTKADIV
                     DINMGCPVNKVVKNEAGAKWLRDPDKIYHIVKEVTSVLDIPLTVKMRTGWADSSLAVE
                     NALAAESAGVSALAMHGRTREQMYTGTCDHETLARVSKAITKIPFIGNGDVRSVQDAK
                     FMIEEIGVDAVMIGRAAMNNPYLFTQINHFFETGQELPDLPFAKKLDIAKDHLKRLIN
                     LKGETIAVREFRGLAPHYLRGTAGAAKVRGAVSRAETLAEVEAIFKTVR"
     gene            complement(29368..30240)
                     /gene="hslO"
                     /locus_tag="VC394_000797"
     CDS             complement(29368..30240)
                     /gene="hslO"
                     /locus_tag="VC394_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEP5994277.1"
                     /translation="MDKIIKSIAQSGAFRAYVLDSTETVALAQEKHNTLSSSTVALGR
                     TLIANQILAANQKGDSKITVKVIGDSSFGHIISVADTKGHVKGYIQNTGVDIKKTATG
                     EVLVGPFMGNGHFVTIIDYGTGNPYTSTTPLITGEIGEDFAYYLTESEQTPSAIGLNV
                     LLDENDKVKVAGGFMVQVLPGASEEEIARYEKRLQEMPAISHLLASKNHVDALLEAIY
                     GDEPYKRLSEEPLSFQCDCSRERFEAALMTLPKADLQAMIDEDKGAEIVCQFCGTKYQ
                     FNENDLEALINDKA"
     gene            complement(30387..31880)
                     /gene="rofA"
                     /locus_tag="VC394_000798"
     CDS             complement(30387..31880)
                     /gene="rofA"
                     /locus_tag="VC394_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047149466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RofA"
                     /protein_id="HEP5994278.1"
                     /translation="MIEKYLESSIESKCQLIILFFKTSYLPITEVAEKTGLTFLQLNH
                     YCEELNAFFPGSLSMTIQKRMISCQFTHPFKETYLYQLYASSNVLQLLAFLIKNGSHS
                     RPLTDFARSHFLSNSSAYRMREALIPLLRNFELKLSKNKIVGEEYRIRYLIALLYSKF
                     GIKVYDLTQQDKNTIHSFLSHSSTHLKTSPWLSESFSFYDILLALSWKRHQFSVTIPQ
                     TRIFQQLKKLFVYDSLKKSSHDIIETYCQLNFSAGDLDYLYLIYITANNSFASLQWTP
                     EHIRQCCQLFEENDTFRLLLNPIITLLPNLKEQKASLVKALMFFSKSFLFNLQHFIPE
                     TNLFVSPYYKGNQKLYTSLKLIVEEWMAKLPGKRDLNHKHFHLFCHYIEQSLRNIQPP
                     LVVVFVASNFINAHLLTDSFPRYFSDKSIDFHSYYLLQDNVYQIPDLKPDLVITHSQL
                     IPFVHHELTKGIAVAEISFDESILSIQELMYQVKEEKFQADLTKQLT"
     gene            32111..>33070
                     /locus_tag="VC394_000799"
     CDS             32111..>33070
                     /locus_tag="VC394_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioester-forming surface-anchored protein"
                     /protein_id="HEP5994279.1"
                     /translation="MVSSYMFLRGEKMNNKIFLNKEAGFLAHTKRKRRFAVTLVGVFF
                     MLLACAGAIGFGQVAYAADEKTVPNFKSPDPDYPWYGYDAYTGAFLRYHDLRVNLNGS
                     RSYQVYCFNIKKNYPRPFTSSDKKWYKRLEGTAETFKVHAMAPRVGGEELTKKLRSVM
                     YNGYPNDGNNIMKGLEPSNAIEVTQYAVWYYSDKSQYEIETLWKSEAKEGKISESQVT
                     LMREALRKLISSDEDLVKQVPSNFKLSTFESSDKSYQNLLSAEFVPDDPPKPGDTSEH
                     NPKTPELDGSPIPEDPKHPDDNLEPTLPPVMLDGEEVPEVPSES"
BASE COUNT         9906 a         5956 c         7198 g        10010 t
ORIGIN      
        1 atggtccgtt ggtcaagggg ttaagacacc gccttttcac ggcggtaaca cgggttcgaa
       61 tcccgtacgg actatattat atttggagga ttacccaagt ccggctgaag ggaacggtct
      121 tgaaaaccgt caggcgtgta aaagcgtgcg tgggttcgaa tcccacatcc tccttttaac
      181 tatcgcggga tggagcagtt aggtagctcg tcgggctcat aacccgaagg tcgtaggttc
      241 aaatcctgct cccgcaatac ataagtatgt agttgttggc tcggtagctc agttggtaga
      301 gcaatggatt gaagctccat gtgtcggcgg ttcgattccg tctcgcgcca ttaaaattta
      361 atattttacg gaaagatagc gaagaggcta aacgcggcgg actgtaaatc cgctccttcg
      421 ggttcggggg ttcgaatccc tctctttcca tcctaaacgg gcatagttta aaggtagaac
      481 taaggtctcc aaaaccttca gtgtgggttc gattcctact gcccgtgtta gaaatatggc
      541 gggtgtggtg aagtggttaa cacatcagat tgtggctctg acattcgtgg gttcgattcc
      601 catcactcgc ctattttatt ggggtatagc caagcggtaa ggcaagggac tttgactccc
      661 tcatgcgttg gttcgaatcc agctacccca gtttaaattt tatatttaaa gccggcgtgg
      721 cggaattggc agacgcgctg gactcaaaat ccagtgtccg caaggacgtg ccggttcgac
      781 cccggccgcc ggtatacttg aaaaacaagg tttacaaacc ttgttttttg ttttgatctt
      841 aagctgtagc tacgtagtac aatctcttat attactgata aggtctattt aatgcgcaag
      901 atttgtggta tgatgaatat gattgagtat tgatgcttta tgggattacc ggtaaggttt
      961 tgaaaaaggt ttgtcactac taggtgcttt tttagctata gaaagacagt tatggattga
     1021 tgtattgtaa ctaatgttgg aggttaatgg tggttgagtt ttgagatatt tacactgata
     1081 atcgggtgga aactggttgg gtatcgggta atggagtgtg gttcttcgtt taagcaagga
     1141 gattatcgtt tggtggttca tatttgcttg ttaaatgtta gggatgagat gctgagtcag
     1201 caaggccatg aagataaagc tgactggcca gagccttgag atgtgacgtt tggtggaagt
     1261 cctttttggt gagatggcac agcatatagt aatattagat cctgaggaag aattgggact
     1321 ttcattaggc ttgactgggg tatgacctca ctttacaatt catttcgatg atgggtttga
     1381 tgatacgttt ttagttcagg actttgtaga gttggagaat ttggttcttt atgaggaaga
     1441 agtgcaggta attcgttgag acagtcgtga agatatttta ggtatgattg atgaaggttt
     1501 gttcattcct tatctgaagt ataaaataaa tttgtgcttt gagatggttg gccaatataa
     1561 agtccatcaa tcttagaagt aataattttt gaattttttc tgaggtttgt gtatatttga
     1621 tagtgtactg atgtaagaag taaggagtac tatgatggca atcaatgaga gagcttctgt
     1681 aaaaatcaag ttaggccttg atactaagaa taagcgatga gatggttacc acgatttatc
     1741 gatggtcatg atgagtattg atctgtgtga ctatgtgacg gttgaccata tagatgacaa
     1801 taagattgtc tttgcctcaa actgtccgaa aatacctatt aactaagata atgatgttta
     1861 taagatagtt cagctaatga aacatcgatt tcaagttaaa agaggagttt ccgtttactt
     1921 ggaaaagcgg attcccatgt gtgctggtat gggaggaggt tctagcgatg ctgttactat
     1981 cagagcatta aatcagttat ggctccttac tcttagccga aaagatatga tggatattgg
     2041 tataccgatt ggtagtgatg tgccttattg cttgctttca ggtcgtgccc aagtcactgg
     2101 taaaggtgaa gtggtttgtc gcattttggg gctgttatcg tcttgggtgg ttttggttaa
     2161 accagatttt ggtatctcga cttgaacatt ttttctagat attaattgta aaatcatttc
     2221 aagagtgtct actactcatt tggttgcggc gattgaagcg ggtaactata atgatgggat
     2281 attgactgaa atgaacaatt tgctggaaga tattttcatt gccaagcgac cctttattca
     2341 aaaaataaaa gagaaaacac tgcaagctgg tgctgctact actttgatga caggaagtgg
     2401 accgactgtt tttgctttgt gccagaccga aaaacaaact ataatagttt aaaaggactt
     2461 tgcaaggaag tttttaaggt gagaatgttg taatattttg ttgggattgc ttctttggaa
     2521 gcttcatgtt ataattaact ggttaatgaa agaggagatt tatggggact ttagaaaaaa
     2581 aacttgataa cttagtaaat actattttat taaaagcaga aaatcagcat gagttattat
     2641 ttggagcttg tcaaagtgac gttaagctta ctaatacgca agaacatatt ttaatgttac
     2701 tatctcagca acgtctcact aatacagatt tggctaaggc attaaatatt agtcaggcgg
     2761 cagtaactaa ggctatcaag agtttggtca aacaagacat gctagcagga actaaggata
     2821 cggttgatgc tagggtgact tattttgaat taaccgagtt agctaagcct attgcgtcag
     2881 aacataccca tcatcatgat gaaaccttaa atgtttacaa ccgtttatta caaaaattct
     2941 ccgcgaaaga attagagatt gtagataagt ttgtaacagt ttttgctgag gaattagaag
     3001 ggtaatccat gagatacata tcagtgaaaa atctctcctt tcaatatgaa agtgagccag
     3061 ttttagaagg gatcacttat catttagata gtggagaatt tgtcaccatg accggtgaaa
     3121 atggcgctgc aaagtcaacc ttaataaaag caaccttagg aattttacaa ccaaaggctg
     3181 gacgagttac tattgctaaa aaaaataaag acggtaaaca attaaggatt gcttacttgc
     3241 cgcagcaagt agctagcttt aacgctggtt ttccatccac cgtttacgag tttgtcaaat
     3301 caggtcgcta cccacgtagt ggttggttta gacatttgaa caaacacgat gaagagcatg
     3361 tgcaagcaag cttagaagca gtcggcatgt gggaaaaccg tcataagaga attggtagtt
     3421 tatcaggtgg tcaaaaacaa cgtgtggtta ttgcccgtat gtttgcttct gaccctgata
     3481 tttttgtgct agacgagcca acaacgggaa tggatagcgg tactactgat accttttatg
     3541 aactgatgca ccacagtgca catcaacacg ggaaatccgt tctgatgatt acccatgacc
     3601 cagaagaagt gaaggcttat gctgatcgga acattcattt agtcagaaac caaaaacttc
     3661 cttggcgttg tttcaacatt catgaagttg aaacagatga cgaaaaagga ggtcatggtc
     3721 atgcttgata ttttatccta tgatttcatg caacgggcgg taatggcggt agttgccatt
     3781 agtatttttg ctccgatttt aggtattttc cttattttac gtcgtcaaag tttgatgagc
     3841 gataccctta gtcatgtttc tttggctggg gtagcgcttg gggtagtcct tggtatttca
     3901 ccaaccatca ctactattat tgttgtggtt ttagctgcta ttttgttaga atacctgcgt
     3961 gtagtttaca aacactacat ggagatttca acggcgattt tgatgtcact tggcttggcc
     4021 ctatctctga ttattatgag taagtcgcat agttcatcaa gcatgagttt agaacaatac
     4081 ctttttggat cgatcatcac gattagtatg gaacaagttg tcgccttgtt tgctattgct
     4141 gcgattattt taatcttgac cgttctcttc attagaccga tgtacattct gacctttgat
     4201 gaagatactg cttttgtaga tggtttgccc gttcgcttga tgtctgttct attcaatatc
     4261 gtcactggga ttgctattgc tttgaccatt ccagcagcag gagcactttt ggtttctacc
     4321 attatggtct tgccagcaag tatcgcaatg agattgggta aaaactttaa aacagttatc
     4381 ttactgggaa ttgtcatcgg ttttagcggt atgttatctg gtattttctt atcttatttc
     4441 tttgaaacgc cagctagtgc cactattacc atgattttca ttagtatttt cctcttagtt
     4501 agtctaggtg gaatgcttaa aaaacggtta ttttaataag aaaaatcctc acatgtttaa
     4561 catgtgagga tttatttata aatgctttaa aaagggggta aggtcccaat tttcttgatt
     4621 aatggtgatg ctctcccaaa agctatcatt agttaaacga ggaatcaaat gaaggtgaaa
     4681 atgagtcccc tcatccatca actccttgtc attgctgacg ctagtgacgc ctgaaatggg
     4741 aaggtgctgc tctagcttag caactagagc ggcttgtaag tcgatgagat catagcgcac
     4801 ttcctttggt agttgtgtca gactagtgta atggtctttg gaaatcagta aaaggtgccc
     4861 cttttggata ggatcaatat caaaaacaac cttgaaatat tgactttgag tcagtagctg
     4921 atttgctttg agctgatggc aaaagataca ttctgtcata ggtacatctc cttatgaaaa
     4981 aacgagccag agctcgttta atcggttatc tagtaagtga gaacagcgta ttttttctta
     5041 ccgcggcgaa taacggttaa ttgatcatca attttatcat cattacttaa ttggtaatct
     5101 aaatcttgaa cgcggtctcc gttaatgtag attgctccat tttgcacgtc ttcacgcgct
     5161 tggcgttttg atggggaaat acctgcggtg acaagcatgt caacaaggtt aaggctgtct
     5221 tctgattgaa cgtgataatt tggtacattg ctcaatcctt gttttaattc attagcagaa
     5281 agatttttga tgtttccagc aaataactgc tcggtaatgt taagagcttg tttataagct
     5341 tcttcgccat gaaccaaggt aaccacttcg cgtgccaagg ttttttgagc gagacgctcg
     5401 tgacgagcgg cattaaattg agtttcaatt tctgcaatct catctagaaa taagaaggtg
     5461 aagattttca agaaacgcac tgcatcatca tccatgacat ttaaccagaa ttggtacatc
     5521 tcgtaaggag acgtcttatc ggcatcaagc cagacggcat taccttctga tttaccgaat
     5581 tttttcccgg ttgagtcagt gatgagtggt acagtcatga cgtgcccagt tttatcagcc
     5641 tttttgcgga gtaattcagt accagctgtc atattgcccc actggtcaga gccgccaatt
     5701 tgtaaggtta cattatgctt gtcattgagt tcgtagaagt cgtagccctg catgatttgg
     5761 taagcaaact cagtgtaaga aatgcctgtt tcaatgcgtt ttttaacaga gtctttactc
     5821 atcatgtagt taacggtgaa gtatttaccg acatcacgga ggaagtcaat aaagctgatt
     5881 tgcgagaacc agtcatagtt gttgacaagc tctgctttat tatcaccatt ttcaaaatca
     5941 aggaaagtag acaactgccc tttaatcttg tcactccact ccaaaactgt ttctttggtt
     6001 tgaaggctgc gctctgcatc tttaaaggaa ggatcaccaa ttaaacctgt ggcaccaccc
     6061 acaagagcgt aaggtttgtg ccctgctagt tgcaagcgac gagatgttaa gatagccact
     6121 agatggccca gatgaaggct gtcagcggtt ggatcataac cggtataata ggatacttgc
     6181 ccttctgtta atgctttgac aagggcttgt tcatcagtcg tttgaaagac caagccacga
     6241 gctttgagtt cttcaaaaat attcataggc tttctccatt ttcattatag tttcgaatca
     6301 ttatatcaaa aaacgctact gctatgcaag ctttatcaaa gtttggtata atgataaatg
     6361 aggtatttta tggtgaaatg gaacacgaaa caaaagcgta taagtcatca aagattaggt
     6421 ctcttggatt tggggccggt gctattacgt acgttgagac tactgtctaa ctttttttat
     6481 attgttatct tcctttttgg aatgatggga tttggtatgg catttgggta tttggctagt
     6541 cagattgaat ctgttaaggt accaagtaaa gaaagtttag tcaaacaagt tgaatcatta
     6601 acgatgattt cgcaaatgaa ctattctgat aatagtttaa tttctacttt agatacggat
     6661 ttacttcgaa caccagtagc taatgatgcg atttcagaga atatcaaaaa agctattgta
     6721 tcaacagaag acgaacattt tcaagaacat aaaggtgtcg tgccaaaagc tgtttttcgg
     6781 gcaacattgg cttctgtatt gggatttgga gaagctagtg gaggttcgac cttaacacag
     6841 caattggtca aacaacaagt tttgggagac gatcccacat ttaagcgcaa gtctaaggag
     6901 atcgtttacg ctcttgcctt agagcgttat atgtccaaag acaatatctt atgtgattat
     6961 cttaatgttt caccttttgg tcgtaataac aagggccaaa atattgctgg tgttgaagaa
     7021 gctgcgcgtg gcatttttgg cgtttctgcc aaagatttaa cggtgccaca ggcagcattt
     7081 ttggcggggc ttccgcagag tcctattgtt tactctcctt atttgtcaac gggacaactg
     7141 aagtcagaaa aggacatggc ttatggcatc aagcgtcagc aaaatgttct ctataacatg
     7201 taccgtacag gtgttctgtc taaaaaagaa tacgaggact ataaggctta tccgattcaa
     7261 aaggatttta ttcaaccggg aagcgcaata gtaaataatc acgattacct ttattacacg
     7321 gtgttagcgg atgctaaaaa agccatgtat agctatttga ttaagcgaga taaggtgtct
     7381 agtcgtgact tgaaaaatga cgagactaag gctgcttatg aagagagagc cttaacagaa
     7441 ttgcaacagg gtggctatac catcaccaca accattaata agcctattta caatgcgatg
     7501 cagacagcgg cagctcagtt tggtggcctg ttagatgatg gcactggtac agttcaaatg
     7561 ggaaatgtct tgacagacaa tgcgactggt gctgtgttag gttttgttgg tggtagagat
     7621 tatgctctga atcaaaataa tcatgctttc aatacaatta gatcgccagg ttctagcatt
     7681 aaaccgataa tcgcttatgg tcctgctatt gatcaaggtt taatggggag tgctagcgtt
     7741 ttgtctaatt acccaacaac ttactcgagt ggccaaaaaa tcatgcatgc tgatagtgaa
     7801 ggaacagcca tgatgccact tcaagaggcc ctaaatactt cttggaacat cccagctttt
     7861 tggacacaga aattactgcg tgaaaaaggt gtcgatgtcg aaaattatat gacaaaaatg
     7921 ggttataaga ttgcagacta ctcgattgaa agtttacctc tagggggcgg tattgaagtc
     7981 tcggttgctc aacaaaccaa tgcttaccaa atgctttcaa acaatggctt atatcaaaag
     8041 caatatattg tagataagat cactgctagc gatggtacag tcgtttacaa acatgaaaat
     8101 aagccaattc gtattttttc tgcagcaaca gctacgattt tacaagaatt gttgagaggt
     8161 ccgattactt caggcgctac gactactttc aagaaccgtt tggcggctat taatccgtgg
     8221 cttgctaatg ctgattggat tggtaagacc ggaacaactg agaattatac ggatgtttgg
     8281 ctagtcctgt ctactccaaa agttacctta ggcggttggg caggacatga tgacaatacc
     8341 tcattagcgc cattaacagg atataacaat aattctaatt accttgccta tttagctaat
     8401 gccattaatc aggccgattc caatgttatt ggagtagggc aacgtttcaa cttagatcca
     8461 ggagtcatta aggcgaatgt cttgaagtca acaggtttac aaccaggaac tgttaatgtc
     8521 aatggacata ctttttctgt tggtggagaa atgaccacca gtctatggtc ccaaaaagga
     8581 ccgggggcta tgacttaccg atttgctatt ggtggcacgg atgccgatta tcaaaaagct
     8641 tgggggaact ttgggttcag aaaaaattag gttgccattt atcgataaaa agggtataat
     8701 agcaataact atttgtcgtc caatctagct gaaatattgt ccagttagga agaacagcag
     8761 ttaaatcaca ctgataaagt cagatttagc tgctcttttt gtgtctattt ttagaaaaga
     8821 tagggtttgt aacctatatt taaatattct aaaaattaac atttagggca aatgataact
     8881 tagttgcgat ttgctgaaac agcaaagctt aaatagaaga aggagctaaa aacttggcag
     8941 gacatgaagt tcgatacgga aaacaccgta cacgtcgtag cttttcaaga atcaaagaag
     9001 ttcttgattt accaaatttg attgaaattc aaactgactc attccaagat ttcctcgatt
     9061 caggtttgaa agaagtattt gaagatgtac ttcctatttc aaactttacg gatactatgg
     9121 aacttgaatt tgttggttac gaatttaaag aacctaaata tacccttgaa gaagctcgta
     9181 tccacgatgc aagttattct gcaccaatct ttgttacctt ccgtttggtc aataaagaaa
     9241 ctggtgagat taaaactcaa gaagtcttct ttggtgattt cccaatcatg acggaaatgg
     9301 gtacctttat tatcaatggt ggtgaacgta tcatcgtttc tcagttggtg cgttctccag
     9361 gtgtttattt caacgataaa gttgataaaa acggtaaagt tggttacgga tcaacagtga
     9421 ttcctaaccg tggggcttgg ttggaattag agactgactc aaaagacatt gcctacactc
     9481 gtattgaccg tacgcgtaag attccattca caaccttggt tcgtgccctt ggtttctcag
     9541 gtgatgatga aattgttgat atctttggtg aaagcgacct tgttcgtaat accattgaaa
     9601 aagacattca caaaaatcca agtgattctc gtacagacga agcccttaaa gaaatttacg
     9661 agcgtcttcg tccaggtgag ccaaaaactg cagatagctc tcgtagtctc ttgattgcgc
     9721 gtttctttga tgcacgtcgt tacgatttgg ctgcggttgg tcgttacaaa gtgaacaaaa
     9781 aacttaatat caagactcgt cttttgaacc aaatcattgc tgaaaacctt gtggatgctg
     9841 aaactggcga aattttggta gaagctggaa ctgagatgac tcgtagcgtc atcgaatcca
     9901 tcgaagaaca ccttgacggt gatttgaaca agtttgttta cacaccaaat gattacgctg
     9961 tggttactga acctgttgtt cttcaaaagt tcaaggttgt gtcaccaatt gatcctgatc
    10021 gtgtagtaac tattgttggt aatgccaatc cagatgataa ggttcgtgca ttgacacctg
    10081 ctgatatttt ggcagaaatg tcatacttct tgaaccttgc tgaaggtctt ggaaaagttg
    10141 acgatattga ccacttaggt aaccgtcgta tccgtgccgt tggtgaattg cttgccaacc
    10201 aattccgcat cggtcttgct cgtatggagc gtaacgttcg tgagcgtatg tctgttcaag
    10261 acaacgatgt gttaacacca caacaaatca tcaatatccg tcctgtcaca gcagctgtca
    10321 aagaattctt cggttcgtct cagttgtcac agttcatgga ccaacacaac ccattgtcag
    10381 agttgtctca caaacgtcgt ttatctgcct taggacctgg tggtttgaca cgtgaccgtg
    10441 ctggttatga ggttcgtgac gtgcattaca cgcactatgg ccgtatgtgt ccgattgaaa
    10501 cacctgaagg accaaatatt ggtttgatta acaacttgtc ttcatttgga catcttaata
    10561 aatatggttt cattcaaaca ccttaccgta aggttgaccg tgcgactggt agggtaacta
    10621 acgagattgt ttggttgact gccgacgaag aagacgaata cacagttgca caggccaatt
    10681 cgaaactaaa cgaagatggc acttttgctg aagaaatcgt tatgggtcgt caccaaggta
    10741 ataaccaaga attttctgca agtgttgttg atttcgttga cgtttcccct aaacaggtag
    10801 ttgctgttgc gacagcatgt attcctttct tggaaaatga tgactccaac cgtgctctca
    10861 tgggtgccaa catgcaacgt caggctgtgc cattgattga tccaaaagca ccatatgttg
    10921 gtactggtat ggaatatcaa gctgcccatg actcaggcgc tgcggtgatt gctcagcaca
    10981 atggtaaagt tgtcttttct gatgctgaaa aagtggaaat ccgtcgtcaa gatggctcac
    11041 ttgatgttta ccacattacc aaattccgtc gttcaaactc aggaacagcc tataaccaac
    11101 gcacccttgt taaagtagga gacattgttg aaaaaggtga tttcatcgct gatggacctt
    11161 ctatggaaaa tggtgaaatg gctcttggac aaaacccagt cgttgcttac atgacttggg
    11221 aaggttataa ctttgaggac gctgttatca tgagtgagcg ccttgtgaaa gaagatgtct
    11281 acacatctgt tcacttggaa gaattcgaat ctgaaacgcg tgatacaaaa cttggccctg
    11341 aagaaatcac tcgggaaatc ccaaatgttg gtgaagaagc cctcaaagac cttgacgaaa
    11401 tgggaattat ccgtatcggt gctgaggtta aagaaggcga catcttagta ggtaaagtca
    11461 cacctaaagg tgaaaaagac ctctctgctg aagaacgttt acttcacgcc atctttgggg
    11521 ataaatcgcg tgaagtgcgt gatacctcac ttcgtgtccc tcacggtggt gatggtatcg
    11581 ttcgtgatgt gaaaatcttt acacgcgcta acggcgatga attgcaatca ggtgttaata
    11641 tgcttgtgcg tgtttacatc gctcaaaaac gtaaaatcaa ggtcggagat aaaatggccg
    11701 gtcgtcacgg aaacaagggt gtcgtttcac gtattgtacc agttgaagac atgccatacc
    11761 ttccagacgg aacaccagtt gacatcatgt tgaaccctct tggggtgcca tcacggatga
    11821 atattggtca ggttatggaa cttcaccttg gtatggctgc tcgtaatctt ggcattcaca
    11881 ttgcaacacc tgtctttgac ggggcttcat cagaagacct ttgggacact gttcgtgaag
    11941 ctggtatgga tagcgatgct aagacggtcc tttatgatgg tcgcaccggt gaaccatttg
    12001 acaaccgtgt atccgttggt gtcatgtata tgatcaaact tcaccacatg gttgatgata
    12061 aacttcatgc ccgttctgta ggaccatact cacttgttac ccaacagcca cttggtggta
    12121 aagctcaatt tggtggacaa cgttttggtg agatggaggt ttgggccctt gaagcttatg
    12181 gtgcatcaaa tgttcttcaa gaaatcttga cctacaagtc agatgacgtc acaggacgtt
    12241 tgaaagccta tgaagcgatt actaaaggta aaccaattcc aaaaccaggt gtaccagaat
    12301 ccttccgtgt tcttgtaaaa gaattgcaat cgcttggtct tgatatgcgt gtgcttgacg
    12361 aggatgataa tgaagtggaa cttcgtgatc ttgatgaagg tgaagacgat gacattatgc
    12421 atgttgacga tctcgagaag gcacgtgaaa aacaagctca agaaacccaa gaagtttctg
    12481 aaacaactga cgaaaaataa gcaatcaatt cttattaaat aattatttac tggtctgggg
    12541 caaaggcccc aggaactggt aaagtcatca aaggcagaaa ggtaaaacta gtggttgacg
    12601 taaatcgttt taaaagtatg caaatcacat tagcctcacc aagtaaggtc cgttcatggt
    12661 cttatggtga agttaaaaaa cctgaaacaa tcaactaccg tacattaaaa ccagaacgtg
    12721 aaggactctt tgacgaagtc atctttggtc caacaaaaga ctgggaatgt gcgtgtggta
    12781 agtacaaacg tatccgttat aaagggatcg tttgtgaccg ctgtggggtt gaggtaactc
    12841 gtgctaaagt acgtcgtgaa cgtatgggtc acattgagtt aaaagctcct gtatcacata
    12901 tctggtattt caaagggatt ccatctcgta tgggattgac tcttgatatg agtcctcgtg
    12961 cccttgaaga agtgatctat tttgcggctt atgtggtcat tgatccaaaa gatacaccgc
    13021 ttgaaccaaa atcattatta acagaacgtg aataccgtga aaaactacaa gagtatggtc
    13081 atgggtcatt tgtcgctaag atgggtgctg aagccattca agatctcttg aaacgtgtgg
    13141 acttggcagc tgaaatcgct gaattaaaag aagagttgaa atctgcttct ggtcaaaaac
    13201 ggattaaagc agttcgtcgt ttagacgtcc ttgatgcctt taacaaatct ggaaataaac
    13261 cagaatggat ggttcttaac atcctgccgg ttattccacc agatcttcgt ccgatggttc
    13321 aattggatgg tggccgtttt gcggcatcag acttgaatga cttgtaccgt cgtgtgatta
    13381 accgtaataa ccgtttggca cgtttgttag aacttaatgc ccctggcatc attgttcaaa
    13441 atgaaaaacg aatgcttcaa gaagccgttg atgctttgat tgataatggt cgtcgtggtc
    13501 gtccaatcac tggaccagga agtcgtccat tgaaatcatt gagccacatg cttaaaggta
    13561 aacaagggcg tttccgtcaa aacttgcttg gtaaacgtgt agacttctct ggacgttccg
    13621 ttatcgctgt tggtccaacc cttaaaatgt atcaatgtgg tgtcccacgt gaaatggcta
    13681 tcgagctttt taaaccattt gtaatgcgcg aaattgttgc caaagaatat gctggtaacg
    13741 ttaaagccgc taaacgtatg gttgaacgtg gcgacgaacg catctgggat atcttagaag
    13801 aagttatcaa agaacaccca gtcctactta accgcgcacc gacccttcac agacttggta
    13861 ttcaggcttt tgaaccggtt cttattgacg gtaaggcact tcgtcttcac ccacttgtgt
    13921 gtgaggccta caatgccgac ttcgatggag accaaatggc catccacgtg ccactttcag
    13981 aagaagcaca agctgaagct cgtcttttga tgcttgctgc agagcacatc ttgaacccta
    14041 aagatggtaa accagttgtt actccatctc aggatatggt tcttggtaac tactatctta
    14101 cgatggaaga tgctggtcgt gaaggcgaag gtatgatttt caaggataaa gacgaagctg
    14161 tgatggcata tcgtaatggt tatgctcatc ttcatagtcg tgtgggtatc gctgttgaca
    14221 gcatgccaaa caaaccttgg aaagacagtc aaagacataa aatcatggtg acaactgttg
    14281 gtaagattct ctttaacgat atcatgccag aggaccttcc ttacctccaa gagccaaaca
    14341 atgccaactt gacagaagga acacctgata aatacttcct tgaacctggt caagacatcc
    14401 aagaagtgat tgatcgctta gacatcaatg tgccatttaa gaagaaaaac ctcggtaaca
    14461 tcattgcgga aaccttcaaa cgtttccgta caacagaaac atcagccttc cttgaccgct
    14521 tgaaagacct tggttactac cattcaaccc ttgccggttt gacagtgggt atcgctgaca
    14581 tcccagttat cgataacaaa gcagaaatca ttgatgctgc tcaccatcgt gttgaagaaa
    14641 ttaacaaagc cttccgtcgt ggtttgatga cagatgatga ccgttatgtt gccgttacaa
    14701 caacatggcg tgaagctaaa gaagcccttg aaaaacgtct aattgaaaca caagatccta
    14761 agaacccaat cgttatgatg atggactcag gagctcgtgg taacatctct aacttctcac
    14821 agcttgctgg tatgcgtggt ttgatggctg ctcctaacgg acgcatcatg gaacttccta
    14881 tcttgtcaaa cttccgtgaa ggtttgagcg ttttggaaat gttcttctca acccacggtg
    14941 cacgtaaagg gatgaccgat acggccctta aaacagccga ctcaggttat cttactcgtc
    15001 gtttggttga cgttgcccaa gatgttatca ttcgtgagga cgattgtggc actgatcgtg
    15061 gtcttcttat ccgtgctatt acagatggta aagaagttac cgaaacgctt gaagagcgtc
    15121 ttcaaggtcg ttacacacgt aaatcagtca aacaccctga aactggtgaa gtcttgattg
    15181 gtgctgacca attaatcact gaagacatgg ctcgtaagat tgttgatgca ggtgttgaag
    15241 aagtgaccat tcgttctgtc tttacctgtg cgactcgtca tggtgtctgc cgtcactgtt
    15301 atggtatcaa cttggcaact ggtgatgctg ttgaagtggg tgaagcagtt ggtactattg
    15361 ctgcccaatc tatcggtgag cctggtactc agcttaccat gcgtaccttc cacacgggtg
    15421 gtgtagcctc aaataccgat atcacacagg gtcttcctcg tattcaagag atctttgaag
    15481 cacgtaatcc taaaggggaa gcggtcatta ctgaagtgaa agggaatgtc gtcgagattg
    15541 aagaagatgc gtcaactcgt accaagaaag tctacgttca aggaaaaact ggcatgggcg
    15601 aatatgtcgt accatttaca gcacgtatga aagttgaagt tggcgacgaa gttaatcgtg
    15661 gagctgccct tacagaaggg tcaattcaac cgaaacgtct ccttgaagtg cgtgatacct
    15721 tgtcagttga aacgtacctt cttgcagaag tacaaaaagt ttaccgtagc caaggggtag
    15781 aaatcggaga caaacacgtt gaggtaatgg ttcgccaaat gcttcgtaaa gttcgtgtca
    15841 tggatccagg tgatacagac ctccttccag gtacacttat ggatatttct gatttcacag
    15901 atgctaacaa agatattgtt atctctggtg gtattcctgc gacatctcgt cctgttctta
    15961 tgggtattac taaggcttcc cttgaaacca attccttctt atcagctgca tccttccaag
    16021 aaacaactcg tgttcttaca gatgctgcta tccgtggtaa gaaagaccac ttacttggtc
    16081 ttaaagaaaa tgttatcatt ggtaaaatca ttccagctgg tactggtatg gcgcgctacc
    16141 gtaacattga accacaagcg atgaatgaga ttgaagtgat tgatcataca gaagtctcag
    16201 cagaagccgt ttttacagca gaagcagaat aatctcatga aagagtcctt aggggctctt
    16261 ttttgtctga gtttgttggc agtaaaaagc attgacaatc agtcttagtc ccaatgagca
    16321 gatgtttttc aaaatactta tgatttagct ataataatgc ataagaggaa taggtaatgt
    16381 gttatgtatc aagtaattaa aatgtatggt gattgggagc cttggtggtt catcgatgga
    16441 tggcaagacg atattataga tgagcaacaa tttagtgact ggcaagaagc gcttgattat
    16501 tttaatcaag aatggcagcg catgaaagct atttttccta gttatcatag tcaaaagaat
    16561 ttgttagcta ctttttggga aaaagaagat aaaagatggt gcgaggactg tgacgaagat
    16621 ttacagcagt ttcattctct tttactcctt aaaaataaag atattgtacc aagcaataat
    16681 tatatccctg aatttgaaca acgaaatgat tcaccacagg tagcttatct ttgcaagtta
    16741 aacctttaag tgtttgttaa aaacaaaata agttaaaaga aaatatcact tcttctgata
    16801 ttttcttttt ttgtttacga ataaatgagt aaggaggaat tatggtacaa gcattagcaa
    16861 aagctattct agcaaaagct gaacaggttc aggcacaaga tatttatatt ttgccaagag
    16921 cagatcaata tgatcttttt ttacgaatag gagatgaaag gagattagtt gatgtttatc
    16981 agagcgatcg gatggctcct cttattagtc actttaaatt cgttgcagga atgatagttg
    17041 gtgaaaagag acgttgtcag gtgggttcat gtgattataa gcttagtaaa gataagcagt
    17101 tatctttgcg cttatctagc gtgggtgatt atcgcgggca agaaagctta gtgattcgtc
    17161 tgcttcatca tcaaaataaa agtgtacatt attggtttga tggattgaca aaagtagcca
    17221 atcaggttgg cggtagaggg ttgtatttat ttgcaggacc agttgggtct gggaagacaa
    17281 ccttgatgta ccagctgatt tcgagttatc atcaagaagc acaggttatt agtatagaag
    17341 atcctgtaga aattaaaaat caccagattt tacaattaca agtgaatgat gatattggta
    17401 tgacttatga caatttgatc aaactgtctt tacgccatcg accagatatt ttagttattg
    17461 gtgagattcg agatagtcaa acagcaagag ccgttattag ggctagtcta acaggtgcca
    17521 tggttttttc aacggttcac gctaaaagta tctcgggtgt ttatgtaaga ttgttagaac
    17581 ttggtgtaac gaaagcagaa ctgtctaatt gcttagcatt aattgcttac caaaggttac
    17641 ttaatggagg agcattgatt gactctactc aaaacgaatt tgaatattat tcctcatcga
    17701 actggaatca acaaattgat cagcttcttg aggcaggaca tctcaatccc aagcaagcta
    17761 agcttgaaaa aattatctag taaacatcag cataaattca ttcaattact agctaatctt
    17821 ttatctacag gatttagctt cgcagaggtt attgcttttt taaaaagaag ccagttgctt
    17881 cagctagatt atgttcttaa aatggaagag tctttattaa aaggacaaga cttagcagat
    17941 atgttgtcgg gtttaggttt ttcagatgct attcttactc aaattagttt agctgataga
    18001 cacggtaata ttgaaacaac attagtagcg attcaacatt acttgaacca aatggcaagg
    18061 atcagacgaa aaactgttga agttatcacc tatcctctta ttcttttact ttttcttttt
    18121 gtgatgatgc tagggttgag acgctattta gtccctcaat tagaaactca aaatcagata
    18181 acttactttc ttaaccattt tcctgccttc tttataggtt tttgctcagg tcttattttg
    18241 ttgttcggga tggtatggct acgatggcga tctcaaagcc gcttgaaact ctatagtcgg
    18301 ctaagccgtt atcctttttt aggtaaactg ttaaaacaat acttaactag ttattatgct
    18361 agagaatggg gtacgttaat aggtcaaggt cttgatttga tgactatttt agacattatg
    18421 gccattgaga aatcctcact aatgaaagaa ttagctgagg acatcagaat gagtctgctt
    18481 gaagggcaag cctttcacat taaagtagct acctaccctt tttttaaaaa agaattaagt
    18541 ttaatgattg aatacggtga aatcaaatcg aaactggggg ctgagttgga aatctatgcc
    18601 caggaaagct gggaacaatt ttttagtcag ctttatcaag tgacacaact tattcaacca
    18661 gctatttttt tagtagttgc tgtgacaatc gtcatgattt atgcggcaat cctattacca
    18721 atttaccaaa atatgggagg tattttttaa gatgattaat caatggaaca acttacgaca
    18781 caagaagcta aaaggattta ctcttctaga aatgttattg gtgattcttg tcatcagtgt
    18841 tttgatgcta ttatttgtgc ctaatttaag caagcaaaaa gacagggtta cagaaacagg
    18901 taatgccgct gttgttaaat tagtggagaa tcaagcagaa ctatatgaat tatctcaagg
    18961 ctcaaaacca agtttgagcc agttaaaggc agatggtagt atcactgaga aacaagaaaa
    19021 agcttatcaa gactattatg acaaacataa aaatgaaaaa gcccgtctta gcaattaaag
    19081 cttttacttt gcttgaaact ttactaagtt tgtcagtgat gagtttcatt atattgggat
    19141 tgtctgtacc agtgacaaaa agctatcaaa aagtagaaga gcatctgttt tttagccatt
    19201 ttgaacattt gtatcgacat cagcaaaagt tagccatatt acagcaaaaa cagcgcgtct
    19261 tggatatttc ttcaacaaaa attgttacag aaggtaatag cctaacagtt cctaaatcaa
    19321 taacagtgaa tcatccttat cggttagtta ttgatcagat gggaggcaac cattccttag
    19381 ctaaaattat ttttgacatg actgatcgac gctttaaata ccaattttat ctagggagtg
    19441 ggaattatca aaaaacaagt caaagcttac atagcccttg aaagtatgat cgcaacaggc
    19501 atcctcttta gtattgtcat tctcgtctta agcagtttac agcagagtca ggctgctcta
    19561 acgtactatc gaaagcagca agaaaagctt aatctagcct tgatggcagt acaaactaga
    19621 actaaagaga taacattgaa tggttgtcat attactattt tacgtacgga tagatacatt
    19681 agtattcacg atgacgaagg agaagtgatg aagattgagt aaacaattaa gtaacataaa
    19741 agcttttacc cttctagagg cgttaatagc cttactcgtg atatcagggt ctttattggt
    19801 ttatcaaggt ttgacccaaa ccctccttaa acgtagccat tatctagccc gtcatgatca
    19861 agacaattgg ctcttatttt ctcatcaatt gcgagaggag ttaagtggag caagatttta
    19921 caaagtagct gataataaac tatacgttga aaaaggaaag aaagtactag cttttggcca
    19981 atttaaaagt catgattttc gaaaatcagc tagtaatgga aaagggtatc aacccatgtt
    20041 atttggaata tcacgtagtc atattcacat agagcagtca cagatttgca ttactttaaa
    20101 gtggaaaagt gggttagaaa ggacttttta ttatgccttt caagactaat ctcaaagcag
    20161 gcatcctttt gtatgctatg tttatggcta gtattttttt gctggtttta caggtttacc
    20221 tttctcaagt gacagctcta cacaaggaat accaagctca aactgactat gttaaagccc
    20281 gactgatagc agaaatagtt tatcaagatc atagatataa agcttctaat cctgtctttt
    20341 ttaaaggtgg tcaagtcatt tgtcgagagc gaaaagagag atggatgtta attgttaagt
    20401 tagatcagca acgacagtat cagtttgaat accttaaacc caaacaatat agtcaataaa
    20461 tagtattgtt gtcataaatc cataaaatga tccaatatat agtaattaaa ggtgacactt
    20521 cttttcattt caagtgtaat ttggtatgat aaggttatga cttttgaaaa aattgaagaa
    20581 gcttatcagc tgctcttaga gaactgtcaa ctaattgaaa atgatctaaa aacacatata
    20641 tacgatgcca ttgttgaaca aaactccttt tatttagggg ctgagggagc tagtcctcaa
    20701 gttgctcaaa atagtgataa actgaaagcc ttgtgcctga caaaagaaga atggcgtaag
    20761 gcctaccagt ttctttttat taaggcagct cagacggagc aactccaagc caaccatcag
    20821 ttcacaccag atgctattgg cttcattctg ctgtatcttt tggaacaatt gagtgataaa
    20881 gatagcttag aggtacttga gattggaagt ggaacaggga acctagccca aacccttctc
    20941 aacaacacga gcaagagcct tgattatgta gggattgaac ttgatgatct cttgattgat
    21001 ctgtcagcca gtattgctga gataatggat tcttcagctc attttattca agaagatgcg
    21061 gtaaggcctc aattactaaa agaaagtgac attgtcatca gtgacttacc agttggttat
    21121 tatcctaacg atgatattgc caaacggtac aaggtggcta gttcagataa gcatacctat
    21181 gcccatcatt tattaatgga acagtcttta aaatacttga aaaaagacgg ttttgcgatt
    21241 tttctggcac cagtcaattt attgacgagc cctcagagcc agttattgaa acagtggtta
    21301 aaagattatg ctcaggtggt gaccttgatt acgctaccag attctatttt tggtcatccc
    21361 tcaaatgcca agtccattat tgtcttacaa aaacaaacag accacccaat ggaaaccttt
    21421 gtctatccaa ttcgggattt gaagcttgca gagaatattc atgattttat ggaaaatttc
    21481 aaaaaatgga aactgagtaa tgtcaattaa ttgatttttt gttataataa aaaagcatca
    21541 cagaaaacga tttcaagaga ggttaaaaat gtcaaagaca attgcaatta atgcaggtag
    21601 ttctagttta aaatggcaac tttatcagat gccagaagaa gaagtgttag cacaaggaat
    21661 tattgagcgt atcggtctca aggattctat ttcaacagtt aagtacgatg gcaaaaaaga
    21721 agagcaaatt cttgatattc acgatcatac agaagctgtt aaaatcttat tgaacgattt
    21781 gattcacttt ggaatcattg cagcttatga tgaaattaca ggagttggac accgtgttgt
    21841 tgctggtggt gaacttttca aagaatccgt tgtggttaat gacaaagttc ttgaacagat
    21901 tgaagaatta tccgttttag ctccacttca caatcctgga gcggcggcag ggattcgtgc
    21961 cttccgtgat attttgccag atattaccag tgtttgtgtg tttgacacgt catttcacac
    22021 tagcatggca aagcatactt acctttaccc aattcctcaa aaatattata cggactacaa
    22081 ggttcgtaaa tatggggcgc atgggacaag tcataaatat gtagcgcaag aagctgctaa
    22141 aatgcttggt cgtccattag aagaattaaa actaattact gctcatattg gaaatggtgt
    22201 ctcaattaca gctaattacc acggtaaatc agttgatacc tcgatgggct ttacaccact
    22261 tgctggtcct atgatgggaa cacgttctgg tgacattgac ccagctatca ttccttattt
    22321 aattgagcaa gacccagaat taaaagacgc tgctgacgtt gtgaatatgt taaacaaaaa
    22381 atcaggtctt agtggggtgt caggcatttc tagtgatatg cgtgatatcg aggctggttt
    22441 acaagaagat aatccagatg ctgttttagc ttacaatatc tttattgatc gcattaaaaa
    22501 atgcattggt caatatttcg ctgttttgaa tggagcagat gctttggtct ttacagctgg
    22561 tatgggtgaa aatgcaccat tgatgcgtca agatgttatt ggtggcttaa catggttcgg
    22621 aatggacatt gatcctgaga aaaatgtttt tggataccgt ggtgaaattt caactcctga
    22681 gtcaaaagtg aaggttcttg ttatctcaac agacgaggaa ttgtgcatcg cgcgtgacgt
    22741 tgagcgtttg aaaaatacaa aataaaagag actaagtttt catagattaa ccaagtaagg
    22801 caacacttta cttggttttt tatgaggttt atctgttagt tatggtgcct aggtattagt
    22861 tctgatcagt ctccttgctt tttgtaatct tgtgctaggc gatttggatg tcagccttat
    22921 ttagaagttt ttaattgctt caacgctttt cttgtatgtg tttatagagc ccaatgatct
    22981 aaaagactat ttgataacgg tttatgatga gcgtaatcgt gcgattcgaa aacagacaca
    23041 agtttactgc ttttacctga tgattgttct atttgttgcc ttattctttt tcttcccttg
    23101 cttaaccatt cgagaccttg tttcttataa gctagctttg atttttgctt acctttactt
    23161 gattatcaga agtctggtgg ctaagaaaat ggaggcacta gggaaaataa caactaatag
    23221 tcaaaaacct ccgtttgata cgagtcaggt tctttgttag atgacttgtc taacggttga
    23281 gtttcctgtc tggtcaacgg aggtgtttgt attgttgtct gatttgtatt ggggcactta
    23341 ttttacaagc ttttagcctt atcgatggtt ttgtcaatgg cagagctgac agtagttgtg
    23401 aggccaaggc gttctaactc cattagacca gcaatagttg tgccaccggg gctacaaata
    23461 gcgtcaatga aatcgtgcgg actttgagaa ctggtcttga gattgctggc gctagccaag
    23521 actgtttgag taacaatctc cagcgccttt gctttaggca tgccattctt gacgccagcc
    23581 ttagccaaag cctcaataaa gagataaata taggcaggac ttgaccctgc taaagcggta
    23641 aaggtgtcaa aatccttttc actaatatca aatgtgctac caaagctatc tgttaagttt
    23701 cgaacacgtg cttgtaattc ctgggacacc aaagcatttc ccgttaaagc ggtactgctt
    23761 tggagaattt gtgcattcat atttggcatg atacgtagca gcggaaggtc ttgtcctacg
    23821 aatgttgcta gtcgttgaag ggaaatgcct gctgccatag atataatagg ctgtttgaag
    23881 tgaagcggtt tgagtaccgt ttcaaatagt tgaggcttga tgcctaaaat aacaagatca
    23941 acctggtcaa taaggtcttg gtgggacata gcataaggca gtgttaactg ctctgcaatt
    24001 tccttggacc gttctaaaga tgatcctgaa atgataagtt catggggtgt ttgtttaagg
    24061 cctttgatga tagcgctagc cattttgcca acaccaataa tgccaatttt cattctgttg
    24121 ctccttgtgg gatattacgg ttaatcaggc taacgctagt ttagtaacgt ttaatcaagt
    24181 caacggttga gcgatccagt tttttgataa tggcttgtaa gaaggcttgg gcttctacga
    24241 aatcatccat agcgtagagg gtttgatgag agtgaatgta gcgtgcacag actccgatgg
    24301 ttgttgatgg gacaccacca ttttgaaggt gtgcagcacc tgcatctgtg cctcccttgc
    24361 cacaatagta ttggaaattg acaccagcgt cctcagcagt agtcagtaag aagtcgcgca
    24421 tatctttgag catgacatgg cctgggtcgt aaaagcgcaa taaggtacca tctccgattg
    24481 ttccaggatt gccataaata tcaccagcag gcgaacaatc tactgcgaaa aagagttcag
    24541 ggtcgaactt ggtggttgag acgtgggctc cgcgcagacc aacttcttct tgaacgttag
    24601 cacctgcaat tagggtgttg ttaaggtctt gtccttttaa cgcttcaagc atttctgtta
    24661 tcatgagaac gccatagcga ttgtcccaag cttttgaaat aatatttttt tgattggctg
    24721 ttaggatcgt ttcagattga gggacaataa tatcacccgg tgtaatgcca aagctttcag
    24781 cttctgcctt atccgtaaag ccaccatcaa acacaatatt ttcgatattt ggtagactag
    24841 cagagccatt tgccccacgt aaaaaatggg gaggtaccga tcctgaaata aggggaataa
    24901 cttggccagt gcgtgtgtaa agggtaaacc gttgtgaact gacaacaagt gggttccaac
    24961 caccgatacc aaccacgcgt agcgttccgt caactttgat atcactgacc ataaaaccga
    25021 cttcgtccat gtgcgcagct actaaaatac ggggggcttt ttcagcttta ctatctctga
    25081 taccaaaaat gccaccaagc ccgtctgttt caacacggtc aaccagcggg gttattttgg
    25141 tgcgtaggta gtcacgaacg ctatgttcat agcccgcaat gccatccagt tcggtaactt
    25201 ctttgatttt tgagaataag tctgtcattt ttcatctcca tttaaaaggt ttcagaccag
    25261 ttcaacgcgg tcatctcctt atattctacc actttttaag taagaatggg tttcacagaa
    25321 aagaaaaatc ccagagacta gagatctctg gaagaagtgt ttgtcttttg gcgataacct
    25381 gtttttttag gcagcaagga aatcattaaa atgataaagc acaagctggt taataagaat
    25441 agtagggcgc ttaggatgcg aaaagactct tcactagcga aaagtaagga taatggagtc
    25501 ataaggagaa ccttccattt taggttaatc agtatttttt atagcgtggt taaattaata
    25561 ataaaccaca cctaatcaat gtacttttag tttagttgtt tttactattt ttgtcaatac
    25621 ataacagtag aaatctttag ttttcttatt tcagatcgtc caatttgatc tttttatgat
    25681 aaaatgaggg atatgagtaa aaagaaaata ggtatgattt caggtatctt tggatttagt
    25741 ttagctattg gcctgggaat agttatcaaa gattattgcc aagacaggca gcgccgacaa
    25801 atgacaaggg atttacgcac ctttttttca cctttaggac aaatcgaagt tttatatatc
    25861 aatccttgtc aggtcaaaca agattatatt tcaggtgggg ttgttatgtc aaacggcaaa
    25921 cagtaccaat tcacctatca cagtcgacaa attagttttg aggaggacaa gggatgatca
    25981 gaccgacttc ttatgagtca ttagcggctc ttattgaaaa agaagataag ctggtgttat
    26041 tttttacggc agactggtgt ccagactgtc aatttattta tcctattatg ccagaaattg
    26101 aagcagaact tacagatatg acttttattt gtgttaatcg tgatcagttt atagaggtag
    26161 cacaaaagtg gaatattttt ggtattccta gttttgtcgt gattgaaaaa ggtcaggaag
    26221 tagggcgtct ggttaacaaa atgagaaaga caaaaactga gattatgcat tttttagctg
    26281 cttatcaata agaaaagagg aagaataaat gatttttgca tacaataaag aacaagttgg
    26341 cgatgtcttg atggttatct tacaagacac caaagatatc aaacgtcaag tagaacgaaa
    26401 aggcaaagta gcccgtgttt ttgcagaaga aagcggcaaa acccttgctt ggaatatctt
    26461 tgaagtgtca agcttgatta ctattgaagg caatggacag atttttttga cagacgagga
    26521 ccttgcaaga ttaaatgcag agcttgccaa ggaaggattt tcagaaaggc ttgaaccgac
    26581 tgtgggacct gtttttgtgg ttggtcaaat tgttgagatg gtggctcatc cagatagcga
    26641 ccatcttaat atttgtcaag tggctattgg tgaagatcaa acggttcaaa tcgtagcggg
    26701 cgcaccaaat gctgcgcttg gtttaaaaac gattgttgcc ttaccaggtg ctataatgcc
    26761 aaatggtagt ctgattttcc caggaaaatt gcgtggtgaa gaaagttatg gtatgatgtg
    26821 ctctcctcgt gagttggtct tgccaaatgc accgcaaaaa cgtggcatta ttgaacttga
    26881 tgagtcagct gtggtaggag aagcttttga cccagccaaa cattggaaag gttagaaaaa
    26941 ccagctgaaa taagactgta tttaactaaa tgaccagaaa aaaaggatta ggagccctta
    27001 atcctttttt cttatgtttt caaaaacgtt taaaatgaaa cttattttac cacaattttt
    27061 acgaataata agataaggag gtcagtatgt ataataaagt gatagcaatc ggtcgtttgg
    27121 tagctaaacc agaattggta aaaaccgcta cggataagca tgtagcacgt ctctctttag
    27181 ctgttaatcg aagatttaaa aatgcttcgg gagagcgaga agcggatttt atttcaattg
    27241 ttgtttgggg aaagttagca gaaactctgg tttcttatgc tagcaaaggt agtttgatgt
    27301 ctattgatgg cgaacttagg acccgcaagt atgataaaga tgggcaagtg cattatgtga
    27361 cagaagttct ctgccaatca tttcaactgc ttgaaagtcg tgctcagcgc gctatgagag
    27421 aaaataatgt tactaatgat ctagttgatt tagtcttaga agaagatact cttccctttt
    27481 gatagaggct aatgcttctc atcagggttc agctgattac tgaaatatat gatgaggtga
    27541 tatccgagca acaaaagtgt gactagatac ttctgataaa aataaaaaaa acaccttacg
    27601 agtatgaaca aagtttcaag tgctagatgt tggaactttg ttcatcatag gaatagtagg
    27661 gtgtttttta gtgtattact tctttttgtt gctagtgtta aggtttactt gcttacaagc
    27721 ggttatcagg ccactagaac tcttataata aatggagctc tttgagtttg gtgtcaatca
    27781 tttttaagac agtcgccaaa tcttgtgggt tttgaacgaa atcaagggaa ttgccatcaa
    27841 ttttcatttt tggagagacc ttgtagtctt cgtaccaggt tgggtattca ccgtggactt
    27901 gatggtaata ttgctcaaga ctaggattgc cgtcgacctg ctcaaaactt cggccacgtt
    27961 tttcaatgcg ctcaagcatt ttgtcaaaag acacatcaat gtagatgaga agatcagggc
    28021 gttttttagg catgccttct agttcttcta acatgttggc gagaagttcc tgatagatgt
    28081 caagttctgt cttagtgaca ttgccatttt tgtagttaag ttttaagaag agttcgtctt
    28141 caaaaataga gcggtcaaga atgttgttat cggcttgata agcttctttg atggacttaa
    28201 accgtttatt caaaaaataa atttgaagta aaaatgcgta ctttttaggg tcttggtagt
    28261 agaggtctag cacggggtta ttatcaacag cttcataaaa aacgtcagtt cctaagtgct
    28321 ctccaagtgc agcagcaagc gagctcttgc cggccccaat tgttccagct agtacaatca
    28381 acatgcgtct ccttctaata tcattagcgt actgttttga agatggcttc aacctcagct
    28441 agagtctcag cgcgtgaaac agcaccacga actttggcag cacctgctgt tccgcgaaga
    28501 tagtgtggcg ctaagccccg aaattcccga acagcaatgg tttcaccttt gaggttaatc
    28561 aggcgtttga ggtggtcttt ggcgatgtct aattttttgg caaagggaag atctggcaat
    28621 tcttgaccag tttcaaagaa atgattgatt tgggtaaaaa gataaggatt gttcatggct
    28681 gcgcggccaa tcatcacggc atctacaccg atttcttcaa tcataaattt ggcgtcttgc
    28741 accgaacgca cgtccccatt tccaataaag ggaatcttag tgatagcctt agaaactcgg
    28801 gctaatgtct catggtcgca ggttcctgta tacatttgtt cacgggtacg accgtgcatg
    28861 gcaagagcag atacacctgc cgactcagcg gcaagggcat tctcaacggc aagtgaacta
    28921 tccgcccaac ctgtacgcat tttgacagtc agtggaatat ctaaaacaga cgtgacttcc
    28981 ttgacaatgt ggtaaatctt atcagggtca cggagccact tggcaccagc ctcgtttttg
    29041 acaactttat taacagggca gcccatatta atatcaacaa tatcggcctt ggtattggtt
    29101 tgaatgaaat cagctgcacg tttcaaccct tctgcgtcac caccaaataa ttgaatggac
    29161 atagggtgct cattctcgtc aatatggagc atgtgaaggg tcttttcgtt gttgtagagc
    29221 agcccttttt cggaaatcat ttccataacc accaaaccag caccaaattc tttggcgatg
    29281 gtacgaaagg cagaattggt cacgccagcc ataggggcaa gtacagtgcg gtgggggatt
    29341 tccacatcac caatcctaaa ggaagaatta agctttgtca ttgatgaggg cctccaaatc
    29401 attttcatta aattggtact tggtaccaca aaattggcaa acaatctcag cacccttatc
    29461 ttcgtcaatc atggcctgta aatctgcttt tggcaaagtc attagggctg cttcaaaacg
    29521 ttctctagaa cagtcacact gaaaactcag aggttcttca gaaagacgct tgtaaggttc
    29581 atctccatag atggcctcca aaagggcatc cacatgattt ttggaagcta gtaggtgaga
    29641 aatggcaggc atttcttgca ggcgtttttc atagcgagca atctcttctt cagaagctcc
    29701 tggtagcacc tgcaccataa agccaccagc aaccttgacc ttatcatttt catctaaaag
    29761 cacattaagt ccgattgcag atggtgtttg ctctgattcg gttaagtagt aggcaaaatc
    29821 ctcaccaatt tccccagtaa taagaggcgt tgttgaggtg taagggttac ctgtgccata
    29881 atcaataatc gtcacaaaat gaccattccc cataaaaggc ccaacaagga cttcacctgt
    29941 tgcggtcttt ttaatatcga caccagtatt ttgaatgtaa cctttcacat gtcctttggt
    30001 atctgccaca gagataatgt gtccaaaaga agaatcacca atcactttga cggtgatttt
    30061 actatcgcct ttttgattag ccgccaaaat ctggttggcg atcaaggtac ggccaagcgc
    30121 aacagtagac gatgacagcg tgttgtgttt ttcttgagct aatgcaaccg tctcagtgct
    30181 atcaaggaca taagccctaa aagcccctga ttgtgctatt gatttaatta ttttatccat
    30241 aacttagatt atagcataaa gtgtaaaact tgtattgaca ggattgcttg aaaaatcaat
    30301 acagatagac agtaaaaaat cccagagaat gactcgggat taagttaagg tgtggtctaa
    30361 agtcgcgcaa tgtggtgaca tggtgcttat gttaattgct tggttaaatc agcttggaat
    30421 ttttcctctt taacttgata catcaattct tggatagaca gaatcgattc atcaaaagat
    30481 atttcagcaa cagcaattcc ttttgtaagt tcatggtgaa caaaaggaat cagttgactg
    30541 tgagtgatga ccaaatctgg ctttaaatca ggaatttgat aaacattatc ttgcaataga
    30601 taataggaat gaaaatcaat gcttttatcc gagaaatacc ttggaaaaga atccgttagg
    30661 agatgagcat tgataaaatt actggctacg aaaacaacaa ctaaaggagg ttggatattt
    30721 cttagacttt gctcgatata gtgacaaaaa agatgaaaat gcttatggtt caagtcacgc
    30781 ttaccaggaa gtttggccat ccactcttcg acaattaact ttaaggacgt atagagtttt
    30841 tggtttcctt tatagtacgg agaaacgaat aagttggtct caggaataaa atgttgcaga
    30901 ttaaacaaga atgattttga aaaaaacata agagctttta ctaaactagc cttttgctct
    30961 tttaggttag gtaaaagagt gatgatagga tttaaaagca ggcgaaaagt atcattttct
    31021 tcaaaaagtt gacaacattg tctgatatgc tcaggtgtcc attgtaagct cgcaaaagaa
    31081 ttattagcgg tgatataaat taaatagagg tagtccaaat ctcctgctga aaagtttagt
    31141 tggcagtaag tttcgataat atcatggcta ctttttttca aagaatcata gacaaaaagt
    31201 ttttttaatt gttgaaaaat tctggtttgg ggaatagtta ccgaaaattg atgccgcttc
    31261 cacgataaag ccaataaaat gtcatagaaa gaaaacgatt ccgataacca aggagaggtt
    31321 ttaaggtggg tggaactatg ggataaaaag ctatgaatag tgtttttgtc ttgctgcgtc
    31381 aagtcataaa ctttaatgcc aaacttacta tatagcagag cgatgaggta acggatgcga
    31441 tattcctcac cgacaatctt gttcttagag agttttaatt caaagtttct taataaagga
    31501 atcaatgctt cgcgcatccg ataagctgag gagtttgata aaaaatgact tcttgcaaaa
    31561 tccgtaaggg gacgagagtg ggaaccattt tttattaaaa acgctagtaa ttgtaagaca
    31621 ttagatgatg catagagttg gtaaagataa gtttctttaa aaggatgtgt aaattggcaa
    31681 gatatcatcc ttttttggat ggtcatagac agactaccag ggaaaaaggc attcagttcc
    31741 tcacaataat ggtttagttg taaaaaggtt aagccagttt tttctgctac ctcagttatt
    31801 ggcaaataag atgtcttaaa aaacaagata attaactgac attttgattc gattgatgat
    31861 tccaagtatt tttctatcaa ggaaagggac ctctcttcct aaacttagaa agccaaataa
    31921 gtctttgata agtgtattct cctatttcaa ttctatctaa gctaaaaata gaaatcaaat
    31981 ctaatggttt ttggtgaaaa gtcgaaacct tttgaaatag ctttaaagca atattttctc
    32041 aaaaaatcat tttcaaaaac attaatttgg tgaaaaatat aatcaaaaaa ttgtctttct
    32101 tgacaataac gtggtaagct catatatgtt tttgagagga gagaaaatga ataacaaaat
    32161 atttttgaat aaagaagccg gttttttggc acacacaaaa agaaaaaggc gatttgctgt
    32221 cactttagtg ggagtctttt ttatgctttt ggcatgcgcg ggtgctatcg gttttggtca
    32281 agtagcctat gctgcggatg agaagactgt gccgaatttt aaaagcccag atccagatta
    32341 tccctggtat ggttatgatg cttatactgg agcgttttta agatatcatg atcttagagt
    32401 caatttaaac ggaagtcgga gctaccaagt ttattgtttt aatattaaaa aaaactatcc
    32461 tcgacctttt acgagtagtg ataaaaaatg gtataaaaga ttagagggaa ctgctgaaac
    32521 ttttaaagtt catgctatgg ctcccagagt agggggagag gaactaacta aaaaattacg
    32581 gagcgttatg tataacggct accctaatga cggcaataat attatgaaag gtttggagcc
    32641 ttcaaatgct atagaagtaa cacaatacgc agtttggtac tattcagata agtcacagta
    32701 tgaaattgag acattatgga agtctgaagc gaaagaaggt aagattagtg agtcacaagt
    32761 tactttaatg cgtgaagccc tgaggaaact aatttcctct gatgaagatt tggttaaaca
    32821 agttccatcg aactttaaac ttagtacttt tgagtcttct gataagtcct accaaaatct
    32881 tttaagtgct gagtttgtac ctgatgatcc ccctaaacct ggtgatacgt cagaacataa
    32941 tcctaaaact cccgagttgg atggtagtcc tattcccgaa gacccaaaac atccagatga
    33001 taatttagaa cctacattgc cgccagtgat gctagatggt gaagaagttc cagaagttcc
    33061 aagcgagagc
//
LOCUS       DATXQK010000015        32846 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.015, whole genome shotgun sequence.
ACCESSION   DATXQK010000015 DATXQK010000000
VERSION     DATXQK010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32846
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.015"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(247..2412)
                     /locus_tag="VC394_000800"
     CDS             complement(247..2412)
                     /locus_tag="VC394_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEP5994280.1"
                     /translation="MITIKNPKILKWLKYVLSAILSLIILVIIIGGLLFTFYISSAPK
                     LSEAQLKSTNSSLVYDGNNNLIADLGSEKRENVTADSIPINLVNAITSIEDKRFFNHR
                     GVDLYRIFGAAFHNLTSQTTQGGSTLDQQLIKLAYFSTNESDQTLKRKAQEVWLALQM
                     ERKYTKQEILTFYINKVYMGNGNYGMLTAAKSYYGKDLKDLSYAQLALLAGIPQAPSQ
                     YDPYLHPEAAQNRRNVVLQQMYMEKHLTKAEYETAIATPVAEGLQSLQQRSTYPKYMD
                     NYLKQVIEEVKKETNKDIFTAGLKVYTNIIPDAQQTLYNIYHSGDYVYYPDQDFQVAS
                     TIVDVTNGHVIAQLGGRNQDENVSFGTNQAVLTDRDWGSTMKPITAYAPAIESGVYTS
                     TAQSTNDSVYYWPGTTTQLFNWDLRYNGWMTIQAAIMLSRNVPAVRALEAAGLDYARS
                     FLSSLGINYPEMHYSNAISSNNSSSDKKYGASSEKMAAAYAAFANGGIYHKPRYVNKV
                     EFSDGTSKTFDEKGKRAMKETTAYMMTDMLKTVLTYGTGTAAAIPGVAQAGKTGTSNY
                     TDEELAKIGEKYGLYPDYVGTLAPDENFVGFTKRYAMAVWTGYKNRLTPVYGSSLEIA
                     SDVYRSMMTYLTNGYSEDWTMPNGLYRSGGFLYLSGTYASNTDYTNSVYNNLYSNNTT
                     TASSQTTSDDTSSSNDISNSTNTDNNSNHPSTDDKKTTH"
     gene            complement(2399..3007)
                     /gene="recU"
                     /locus_tag="VC394_000801"
     CDS             complement(2399..3007)
                     /gene="recU"
                     /locus_tag="VC394_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEP5994281.1"
                     /translation="MVNYPHNLIRQKVSSVQKQTKQNKVDFANRGMSFEAAINATNDY
                     YLSRQIAVIHKKPTPVQIVKVDYPKRSRAKIVEAYFRQASTTDYCGVYKGHYVDFEAK
                     ETRQKTAMPMKNFHLHQIEHMACVLHQKGICFVLLHFSTLKETYYLPAQALISFYQID
                     NGSKSMPIDYIRKNGFKVAFGAFPQVPYLNIIEQNFLGGDYN"
     gene            3088..3603
                     /locus_tag="VC394_000802"
     CDS             3088..3603
                     /locus_tag="VC394_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEP5994282.1"
                     /translation="MTAILITGYRSFEIGIFDHKDPRVSIIKQAIRKDLIGYLENGVD
                     WFIFTGNLGFEQWALEVANELKEEYPLQIATIFLFETHGDRWNEKNQEVLSQFRAVDF
                     VKYYFPNYEQPTQFSQYYQFLLEKTEGAYVFYDTENETNLKYFLKKAKDMPHYQLLLL
                     TFDRLNDMSQS"
     gene            3725..4051
                     /gene="gpsB"
                     /locus_tag="VC394_000803"
     CDS             3725..4051
                     /gene="gpsB"
                     /locus_tag="VC394_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEP5994283.1"
                     /translation="MTSIIYSPKDIFEQEFKTSMRGFDKKEVDEFLDNVIKDYENFNA
                     QIEALKAENEALKKAKFQARNTVSATVQQPVPQPTRVAQSATNFDILKRISKLEKEVF
                     GKQIIE"
     gene            4065..4433
                     /gene="rnpB"
                     /locus_tag="VC394_000804"
     ncRNA           4065..4433
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VC394_000804"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4496..5650
                     /locus_tag="VC394_000805"
     CDS             4496..5650
                     /locus_tag="VC394_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEP5994284.1"
                     /translation="MKETFRLVATAAAGLEAVVGKEVRALGFDCQVENGKVYFEGDVE
                     AIVKTNLWLRAADRIKIIVGQFPARTFEELFQGVFALDWGNYLPLGAKFPISKAKCVK
                     SKLHNEPSVQAITKKAVVKKLQKHFHRPEGVPLQEVGSTFNIEVSILKDQATIMIDTT
                     GSSLFKRGYRVQKGGAPIKENMAAAILALSNWFPDKPLVDPTCGSGTFCIEAALIGMN
                     IAPGFNRSFAFEEWSWVDKDMVQQVRDDAEQEANYEIELDISGFDIDGRMIEIAKSNA
                     EEAGLSDVITFKQMRLQDFRTDKVNGVVISNPPYGERLLDDKAVDILYNEMGQTFAPL
                     KTWSKFILTSDELFELKYGQKADKKRKLYNGTLKVDLYQFYGERVKRHLI"
     gene            5664..7133
                     /locus_tag="VC394_000806"
     CDS             5664..7133
                     /locus_tag="VC394_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994285.1"
                     /translation="MSEESKEVEVTKESQTLGLNEAKSMTIGEAVRKQSEIKAGVTKD
                     DSILDKYIKQHRDEVSSQKFDAKYTELDTASLDNFIKKQREALSKAGLVDDEPVSAES
                     AEQDSTLVEEVAEDLAPMETTAVVTGIPVEATVPVLDLDPSERGIPEPQMTKEEPKRD
                     QFLSEDSHHPAKQNTKKGWLIALFLLLLAILAVVFGWNHFLRQDSGKTTQTASKQTKT
                     SLQTDSAKKATRLKAAAKAFEKQYGTFYTDATKSKLKNSAFATLPDLEAALKALEGSA
                     YYDKAKAKVDSLKKAIAAITAVNGKFVSDVVVDGEKVSAEVKADANFDDLSSATLTTG
                     NANLDAVLQASITEGRQQLASKAEAAKAANEQAAQAQAAQGQSTSVAPSGYGLTSYDP
                     ASLQRHLSRVPYNQDVIADRANPSWAFNPGVLEKIVATSQARGYISGYQYILEPVNII
                     NGNGYYNMFKPDGTYLFSINCKTGYFVGNGKGYADALDY"
     gene            complement(7279..7761)
                     /locus_tag="VC394_000807"
     CDS             complement(7279..7761)
                     /locus_tag="VC394_000807"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEP5994286.1"
                     /translation="MTKEVIVESFELDHTIVKAPYVRLISEEFGPKGDRITNFDVRLV
                     QPNQNSIETAGLHTIEHLLAKLIRQRIDGMIDCSPFGCRTGFHLIMWGKHSSTDIAKV
                     IKSSLEEIATGITWEDVPGTTLESCGNYKDHSLFAAKEWAQLIIDQGISDDPFSRHVI
                     "
     gene            complement(7835..9166)
                     /locus_tag="VC394_000808"
     CDS             complement(7835..9166)
                     /locus_tag="VC394_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HEP5994287.1"
                     /translation="MEIIGSLGVLIGVIVIIYLSVKEVNIIIAAPLATSLVILFNQMD
                     PTTTLLGKEPNQFMGALSTYILNYFAIFLLGSILAKLMETSGETTSIADYILKKVGHD
                     SPYKVLVAIFLISAILTYGGISLFVVMFAVLPLARSLFKKMDLAWNLIQVPLWLGIAT
                     FTMTILPGTPAIQNVIPIQYLDTSLTAAAIPSIVGSIGCVTFGLFYMKYCLAKSMARG
                     ETYATYAFDNEIQVKTKNLPHFLASILPLLLLIIIALTGSLFGNDFFKKNIIFIALLA
                     AILTASWLFRQFIPNKIAVFNLGASSSIAPIFATASAVAFGAVVMIAPGFTFFSDLIL
                     NIPGNPLISLAVLTSSMSAITGSSSGALGIVMPNFAQYYLDQGLNPEMIHRVATIASN
                     IFTIVPQSGVFLTFLALTGLNHKNAFKETFITVSVSTFIAQVIMIAFDLFS"
     gene            complement(9237..10016)
                     /locus_tag="VC394_000809"
     CDS             complement(9237..10016)
                     /locus_tag="VC394_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HEP5994288.1"
                     /translation="MTHTKEVAFITGAASGIGKQIGETFLKEGKTVVFSDINQEKLDQ
                     VVADYTKEGYDAFSVVCDVTKEEAINAAIDTVVEKYGRIDILVNNAGLQHVAMIEDFP
                     TEKFEFMIKIMLTAPFIAIKRAFPTMKAQKHGRIINMASINGVIGFAGKSAYNSAKHG
                     LIGLTKVTALEAADSGITVNAICPGYVDTPLVRGQFEDLSKTRGIPLENVLEEVLYPL
                     VPQKRLIDVQEIADYVSFLASDKAKGVTGQACILDGGYTAQ"
     gene            complement(10049..10708)
                     /locus_tag="VC394_000810"
     CDS             complement(10049..10708)
                     /locus_tag="VC394_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEP5994289.1"
                     /translation="MGTSATVLSKEEIQTRIAKRVAQELEHNTLVNLGIGLPTKVANY
                     IPEGVTITLQSENGFVGLTGLTDDHYDPSIVNAGGQPVSIAPGGAFFDSSTSFGIIRG
                     GHVAATVLGALQVDKEASIANYLIPGKMVPGMGGAMDLLVGAKKVIVAMEHTNKGKAK
                     ILDKCTLPLTAQNVVNLIITEMGVFEYQDEGLCALEINPDYTFEDVQNVTEVTLIDKT
                     N"
     gene            complement(10710..11360)
                     /locus_tag="VC394_000811"
     CDS             complement(10710..11360)
                     /locus_tag="VC394_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HEP5994290.1"
                     /translation="MCKEITIKEAVAHVKDGDTIMVGGFMTNGTPEKLIDTLVEKGVK
                     DLTLICNDAGFPDKGVGKMVANKQFSTIIASHIGLNREAGRQMTEGETVIDLVPQGTL
                     AERIRSGGFGLGGFLTPTGIGTEVAKGKEVITVDGKDYLLEKPLKADIALIFANKADK
                     NGNLQYAGSENNFNHVMAANAKTTIVEAREIVDVGQMDPNFVHTPGIFVNYLVKGA"
     gene            complement(11384..12571)
                     /locus_tag="VC394_000812"
     CDS             complement(11384..12571)
                     /locus_tag="VC394_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEP5994291.1"
                     /translation="MKNIVIVEALRTPIGAFGGSFKSVSAVELGTTVLKKILDKTQVK
                     PEMVDEVILGNVLHAGLGQNVARQVAVHSGIPEDKTAFTLDMVCGSGLKAIQLAAQSI
                     MLGDADIVIAGGVENMSQAAYVSTQHRFGQRLGNSQLIDTLVHDGLTDAFNNYHMGIT
                     AENVAQKYGISREEQDQFALESQEKAAKALENHRFADEIVPVSVPQRRKDPLIVTTDE
                     YPKVDTSLEKLQQLRPAFLPKEGTVTAGNASGINDGAALLMLMTEEKALELGLTPLVT
                     IESYASAGVAPELMGTGPIPATQKALKKAGLTISDLDLVESNEAFAAQSLAVLKDLKL
                     NPEIVNVNGGAIALGHPIGASGARILVTLIHEMKKRQVTRGLATLCIGGGQGTAVIVK
                     NNT"
     gene            12772..13668
                     /locus_tag="VC394_000813"
     CDS             12772..13668
                     /locus_tag="VC394_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEP5994292.1"
                     /translation="MDIKQIRYFIAIVENHFNLSQAAELLYVSQPTLSMMINDFEKRE
                     NVKLFKRKRGRIIGLTYLGDNYYKDAQKVLSLYDDMFLKLHDHSKGLKGSISIGIPPL
                     ILSVVFSEVMPKLILENPGIQFNVKEIGAYQLKNELLVGNVDVAVLLSPTGIADNLVE
                     TYEIQRSELSVCLSPRHRLASKKVIQWEDLTDEQLALFDPSFMVHHLVLEACERHQVR
                     PNIILTSSSWDFMLNSTKINHNVLTICPKPITELYQFKDIKCILMERPISWRVVLTRL
                     RKKSYSEIEAYIMDDLLQSFFK"
     gene            13798..15405
                     /locus_tag="VC394_000814"
     CDS             13798..15405
                     /locus_tag="VC394_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEP5994293.1"
                     /translation="MVNIILLIVSALIGLILGYALISIRLKSAKEAAELTLLNAEQEA
                     VDIRGKAEVDAEHIKKTAKRESKANRKELLLEAKEEARKYREEIEQEFKSERQELKQL
                     ETRLAERSLTLDRKDENLSSKEKVLDSKEQSLTDKSKHIDERQLQVEKLEEEKKAELE
                     KVAAMTIAEAREVILMETENKLTHEIATRIRDAERDIKDRTVKTAKDLLAQAMQRLAG
                     EYVTEQTITSVHLPDDNMKGRIIGREGRNIRTLESLTGIDVIIDDTPEVVILSGFDPI
                     RREIARMTLESLIADGRIHPARIEELVEKNRLEMDNRIREYGEAAAYEIGAPNLHPDL
                     IKIMGRLQFRTSFGQNVLRHSVEVGKLAGILAGELGENVALARRAGFLHDMGKAIDRE
                     VEGSHVEIGMEFARKYKEHPVVVNTIASHHGDVEPDSVIAVLVAAADALSSARPGARN
                     ESMENYIKRLRDLEEIATSFDGVQNSFALQAGREIRIMVQPEKISDDQVVILSHKVRE
                     KIENNLDYPGNIKVTVIREMRAVDYAK"
     gene            15515..16150
                     /gene="gmk"
                     /locus_tag="VC394_000815"
     CDS             15515..16150
                     /gene="gmk"
                     /locus_tag="VC394_000815"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEP5994294.1"
                     /translation="MSERGLLIVFSGPSGVGKGTVRQEIFSTPDHKFEYSVSMTTRPQ
                     RPGEVDGVDYFFRTREEFEELIKTGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKSKVPDGVFVFLTPPDLDELEDRLVGRGTDSQEVIAQRIERAKEEIALM
                     REYDYAVVNDEVALAAERVKRIIETEHFRVERVIGRYDKMIKITKNPFKAK"
     gene            16166..16483
                     /locus_tag="VC394_000816"
     CDS             16166..16483
                     /locus_tag="VC394_000816"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEP5994295.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILQAKRAHELEAGATPTQEFKSV
                     KSTLQALEEIESGNVVIHPDPSAKREAVRAKIEAERLAKEEEERKIKEQIAKEKEEEG
                     EKI"
     gene            16548..18932
                     /locus_tag="VC394_000817"
     CDS             16548..18932
                     /locus_tag="VC394_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEP5994296.1"
                     /translation="MEKVAHVIVDIPLMQTDKPFSYGIPKELVSLVQLGSRVHVPFGK
                     GNRLLQGFVIGFGQEDSSSLKLIQTVLDPEPVLNQEQLTLADQLRKTVFSYKITLLKA
                     MIPNLLNSNYDKVLRPESGLKKPDRDFLFEGKPSVLYSTLDREKEKIALKGIQAGHIT
                     VSYLAKDKKNLKTEKYYHVDLDALAVHPISSRAKKRQLLKDYLLTHTKEAKLATLYQA
                     FSRDVVAYFVTNHLIRIDERQIDRSEPYFDQIKPSSFLTLNEQQASAVTEIVEQIGKP
                     SKPFLIEGITGSGKTEVYLHIIEAVLKQDKTAIVLVPEISLTPQMTSRFISRFGKQVA
                     IMHSGLSDGEKFDEWRKIKTGQAKVVVGARSAIFSPLERIGAIIIDEEHESTYKQESN
                     PRYHAREVALLRAKHHQAVVVMGSATPSIESRARASKGVYHFIQLTKRANPLAKIPEV
                     TIVDFRDYIGQQAVSNFTPYLIDKIKERLVKKEQVVLMLNRRGYSSFVMCRDCGYVDK
                     CPNCDISLTLHMDTKTMNCHYCGFQKPIPITCPECHSNSIRYYGTGTQKAFDELQGVI
                     PEARILRMDVDTTRKKRSHKTILDSFGRQEADILLGTQMIAKGLDFPNVTLVGVLNAD
                     TSLNLPDFRASEKTFQLLTQVAGRAGRAHKPGEVLIQTYNPDHYAIQLAKQQDFEAFY
                     RYEMAIRRQMAYPPYYFTVGITLSHRLEASVVKKAYQVTELLKSHLSDNIKILGPTPK
                     PIARTHNLYHYQILLKYRFEDNLEETLNRILDWSQEADNRHLKLIIDCEPQQFL"
     gene            18994..19929
                     /locus_tag="VC394_000818"
     CDS             18994..19929
                     /locus_tag="VC394_000818"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEP5994297.1"
                     /translation="MIKLLFMGTPQFSATVLKGLLDNPAYEILGVVTQPDRAVGRKKD
                     IKVTPVKQLALEHGISIYQPEKLSGSQELIEIMELGADGIITAAFGQFLPTILLDSVS
                     FAINVHASLLPKYRGGAPIHYAIMNGDKEAGVTIMEMIKEMDAGDMVAKASTPILETD
                     NVGTLFEKLAIIGRDLLLDSLPAYLSGELKPIPQDHSQATFSPNISPEQEKLDWTMSD
                     QEVFNHIRGMNPWPVAHTFLEGQRLKIYEAQLAEGEGLPGQVIVKTKKSLVIAAGQGA
                     LSLLVVQPAGKPKMAIADFLNGLGRKLEVGDFIGR"
     gene            19919..21241
                     /gene="rsmB"
                     /locus_tag="VC394_000819"
     CDS             19919..21241
                     /gene="rsmB"
                     /locus_tag="VC394_000819"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEP5994298.1"
                     /translation="MADNWKISIRGKALLTLEHIFDQGAYTNIALNQQLSNKALSAKD
                     RALLTEIVYGTVSRKISLEWYLAHYVKDRDKLDKWVYYLLMLSLYQLIYLDKLPAHAI
                     VNDAVGIAKNRGNKKGAEKFVNAILRQFTSHPLPDMETIKRRNKYYSVKYSLPVWLVK
                     KLEDQFGSDRSVAIMESLFVRSKASIRVTDPLKLEEAAEALDAERSLLSATGLTKASG
                     HFAASDYFTNGDITIQDESSQLVAPTLNIDGDDIILDACSAPGGKTSHIASYLKTGKV
                     IALDLYDHKLELVKENANRLGVADNIETRKLDAREVHRHFEKDSFDKILVDAPCSGIG
                     LIRRKPDIKYNKESQDFNALQAIQLEILSSVCQTLRKGGIITYSTCTIFDEENRQVIE
                     AFLQSHPNFEQVKLNHTQADIVKDGYLIITPEQYQTDGFFIGQVRRVL"
     gene            21279..22019
                     /locus_tag="VC394_000820"
     CDS             21279..22019
                     /locus_tag="VC394_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEP5994299.1"
                     /translation="MKISLKTDIGQKRSNNQDFINKFDNKKGITLVILADGMGGHRAG
                     NIASEMTVTDLGREWVKTDFTELSQIRDWLFETIQSENQRIYDLGQSEDFKGMGTTVE
                     AVALVESSAIYAHIGDSRIGLVHDGHYTLLTSDHSLVNELVKAGQITEEEAASHPQRN
                     IITQSIGQASPVEPDLGVRVLEPGDYLVINSDGLTNMISNDEIVTILGSKVSLDEKNQ
                     EMIDLANLRGGLDNITIALVHNESEDVE"
     gene            22016..23914
                     /gene="pknB"
                     /locus_tag="VC394_000821"
     CDS             22016..23914
                     /gene="pknB"
                     /locus_tag="VC394_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEP5994300.1"
                     /translation="MIQIGKLFAGRYRILKSIGRGGMADVYLANDLILDNEDVAIKVL
                     RTNYQTDQVAVARFQREARAMAELNHPNIVAIRDIGEEDGQQFLVMEYVDGADLKRYI
                     QNHAPLSNNEVVRIMEEVLSAMTLAHQKGIVHRDLKPQNILLTKEGVVKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATIQSDIYAMGIMLFEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSIIEENHNVPQALENVVIRATAKKLSDRYGSTFEMSRDLMTALSYN
                     RSRERKIVFEDVESTKPLPKVASGPTASVKLSPPTPTVLTQESRLDQTNQTDALQPPT
                     KKKKSGRFLGTLFKILFSFFIVGVALFTYLILTKPTSVKVPNVAGTSLKVAKQELYDV
                     GLKVGKIRQIESDTVAEGNVVRTDPKAGTAKRQGSSITLYVSIGNKGFDMENYKGLDY
                     QEAMNSLIETYGVPKSKIKIERIVTNEYPENTVISQSPSAGDKFNPNGKSKITLSVAV
                     SDTITMPMVTEYSYADAVNTLTALGIDASRIKAYVPSSSSATGFVPIHSPSSKAIVSG
                     QSPYYGTSLSLSDKGEISLYLYPEETHSSSSSSSSTSSSNSSSTNDSTAPGSNTELSP
                     SETTAQTP"
     gene            24037..24729
                     /locus_tag="VC394_000822"
     CDS             24037..24729
                     /locus_tag="VC394_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEP5994301.1"
                     /translation="MKKFQFFLLIECILLAMGIMTILDNDLSSFILILVLILLALRFY
                     NQDSRNNFLLTVSLLFLFLIFMLNPYIIMAVLLGIVYIFINHFSQVKKKNRFALIRFK
                     EEKIEVNNTKHQWIGTANYESDYYCFDDINIIRISGNDTVDLTNVIVTGMDNIIVIRK
                     IFGNTTILVPIDVTVTLDVSSIYGSVDFFRCQQYDLRNESIKLKETDNQSLKKVKIIV
                     TTIAGNVEVDRR"
     gene            24726..25730
                     /locus_tag="VC394_000823"
     CDS             24726..25730
                     /locus_tag="VC394_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEP5994302.1"
                     /translation="MKKRYYALVWLYSTITILSIVFVVMDNLGITFNYLRNHLWQVER
                     LGFSILLLIVSVTLLLLLLWIIMDDNSKRNINQNLKYILNNRRLYLDETSEINTNLSR
                     LSKKMSHLTANMQKKESAYILDSQEVVKQERKRIARDLHDTVSQELFASSLILSGISM
                     SLEQLDKTQLQTQLTTVEAMLQNAQNDLRILLLHLRPTELANRTLSEGLHMILKELTD
                     KSDIEVIYKETIAQLPKTMEDNLFRIAQEFISNTLKHAKASRIEVYLNQTSTELQLKM
                     IDDGVGFDMDQVRDLSYGLKNIEDRVNDLAGNLHLISQKGKGVSMDIRLPIVKGDDDE
                     "
     gene            25723..26364
                     /locus_tag="VC394_000824"
     CDS             25723..26364
                     /locus_tag="VC394_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP5994303.1"
                     /translation="MSKIKVILVDDHEMVRMGLKSFLNLQADIDVVGEASNGREGVDL
                     ALALKPDVLVMDLVMPELGGVEATLEVLKKWKEAKVLVLTSYLDNEKIYPVIDAGAKG
                     YMLKTSSAAEILNAIRKVSKGELAIETEVDKKIKAHDQHPDLHEELTAREYDILHLLA
                     KGYDNQTIADELFISLKTVKTHVSNILAKLEVDDRTQAVVYAFRHHLVPQDDN"
     gene            26401..27801
                     /locus_tag="VC394_000825"
     CDS             26401..27801
                     /locus_tag="VC394_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEP5994304.1"
                     /translation="MDAKLKYKAKKIKMVFFDIDDTLRVKDTGYMPESIQRVFKALKA
                     KGILVGIASGRARYGVPQEVQDLHADYCVKLNGAYVKDDAKTIIFQAPIPADVVVAYK
                     KWADDMGIFYGMAGRHEAVLSARNDMISNAIDNVYAQLEVCPDYNEYHDVYQMWTFED
                     KGDGLQLPAELAEHLRLVRWHDNSSDVVLKGTSKALGVSKVVDHLGLKPENILVFGDE
                     LNDLELFDYAGISIAMGVSHPLLQEKADFITKKVKEDGILYALEELGLIDKELQFPQL
                     DLPNHKGPKATIKTNHGDMTLVLFPDHAPKTVANFLGLAKEGYYDGIIFHRIIPEFMI
                     QGGDPTGTGMGGQSIYGESFEDEFSDELYNLRGALSMANAGPNTNGSQFFIVQNSKIP
                     YAKKELERGGWPAPIAASYAAIGGTPHLDRRHTVFGQLVDETSFQVLDLIAGVETGAQ
                     DKPKEDVIIETIEVFD"
     gene            27801..28178
                     /locus_tag="VC394_000826"
     CDS             27801..28178
                     /locus_tag="VC394_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEP5994305.1"
                     /translation="MKIGDKLHGTITGIKPYGAFVALENGTTGLIHISEIKTGFIDDI
                     DQLLAIGNQVLVQVIDIDEYSKKPSLSMRTLAEEKQHFFHRHRYSNSRHKIGFRPLEE
                     QLPQWIEESLQFLKKNTKKSLEP"
     gene            complement(28196..29137)
                     /gene="cysK"
                     /locus_tag="VC394_000827"
     CDS             complement(28196..29137)
                     /gene="cysK"
                     /locus_tag="VC394_000827"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEP5994306.1"
                     /translation="MTKIYKTITELVGQTPIIKLNCLIPNEAADVYVKLEAFNPGSSV
                     KDRIALSMIEAAEAEGLISPGDVIIEPTSGNTGIGLAWVGAAKGYRVIIVMPETMSLE
                     RRQIIQAYGAELVLTPGAEGMKGAIAKAETLAIELGAWMPMQFNNPANPSIHEKTTAQ
                     EILEAFKEISLDAFVSGVGTGGTLSGVSHVLKKASPETVIYAVEAEESAVLSGQEPGP
                     HKIQGISAGFIPNTLDTKAYDQIIRVKSKDALETARLTGAKEGFLVGISSGAALYAAI
                     EVAKQLGKGKHVLTILPDNGERYLSTELYDVPVIKTK"
     gene            complement(29265..29897)
                     /locus_tag="VC394_000828"
     CDS             complement(29265..29897)
                     /locus_tag="VC394_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEP5994307.1"
                     /translation="MEHFKTIKASGFFEESIKKSRFICHIKRVSTEEDGKNFVNAIKK
                     EHYKANHSCFAMIIGNNSQIKRSSDDGEPSGTAGIPILSVLEKQCLTNVVVVVTRYFG
                     GIKLGTGGLIRAYSNMTATAIKRFGIIEVKQQIGLEITLSYPQYQLYSNLLDQLALTE
                     TETKFSDTIKTTLYCDTERVENLIDTLTNYYHGQISCEKIGSKVIEFPCD"
     gene            29953..31278
                     /locus_tag="VC394_000829"
     CDS             29953..31278
                     /locus_tag="VC394_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEP5994308.1"
                     /translation="MESIENSYGRLLLESQLPDSAKQLAQPLKSVVISRGKMICQRCH
                     YQLDEEARLPNGAYYCRFCLVFGRNQSDKLLYAIPPMHFPKGNYLVWSGQLTAYQEMI
                     SQQLLINMQNQKTTLVHAVTGAGKTEMIYAAIEAVINTGGWVCIASPRVDVCVEVATR
                     LSQAFSCSICLMHAESLPYQRAPIIVATTHQLLKFHKAFDLLIIDEVDAFPFVNNIQL
                     HYAASQALKEGGAKILLTATSTRTLERKVNKGEVVKLTLARRFNNRPLVIPKFIRSFN
                     LFKMIHRQKLPPKILKYLKKQRKTGYPLLIFLPTIIMAESVTAILKELLPAEQIACVS
                     SQSQNRKEDITAFRQGKKTILITTTILERGVTFPQIDVFVLGSHHRVYSSQSLVQIAG
                     RVGRSIDRPDGTLYFFHEGISKAMLLARKEIKEMNYKGYSHDLSTMSTN"
     gene            31250..31915
                     /locus_tag="VC394_000830"
     CDS             31250..31915
                     /locus_tag="VC394_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEP5994309.1"
                     /translation="MICLLCQQISQTPISITEIIFLRRISSPICQQCQKSFQKIGKSV
                     CATCCANSDIIACRDCLKWENKGYNVNHRSLYCYNAAMKAYFSQYKFQGDYLLRKVFA
                     VELADVITKYYKGYIPVPVPVSPGCFRERQFNQVSAILEAANVSYLSLFEKLDNTHQS
                     SRTKKERLLVEKSYRLLKVSNIPDKILIVDDIYTTGSTIIALRKQLAKVANSDIKSLS
                     IAR"
     gene            31995..32543
                     /gene="raiA"
                     /locus_tag="VC394_000831"
     CDS             31995..32543
                     /gene="raiA"
                     /locus_tag="VC394_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEP5994310.1"
                     /translation="MIKFSIRGENIEVTEAIRDYVESKLTKIEKYFAKDQEIDARVNL
                     KVYRERSSKVEVTIPLDSVTLRAEDVSQDMYGSIDLVVDKIERQIRKNKTKIAKKHRE
                     KVPTGQVFTTEFEAEEVDEIPEVQVVRTKNVTLKPMDVEEARLQMELLGHDFFIYTDS
                     EDGATNILYRREDGNLGLIEAK"
BASE COUNT        10536 a         5809 c         6673 g         9828 t
ORIGIN      
        1 ttttaggtta acgtttgaaa aatcagttgc taattccctt aacgttgtac cttttcttta
       61 atacgattaa agaattctgt tttttgtttt atattgtatg tggtaagtca tttgtgtcat
      121 ataaaaacga gtcctcacag aacgtggact cgctttttat ttagattatc acttaaggta
      181 gatattaaaa gtaactaaat ggatatgtaa taaaaggtag gtacagacaa taagtagagt
      241 tagagattaa tgagttgtct ttttatcatc ggtagatgga tgattgctat tgttgtctgt
      301 attggttgaa ttacttatat cattgctact actagtatca tctgaagtcg tttggctaga
      361 agctgttgtc gtgttattgc tgtaaagatt gttgtaaacc gaattagtat agtcggtgtt
      421 gctcgcatag gttccgctta agtagaggaa tccaccactg cgataaagac catttggcat
      481 cgtccaatct tcactgtaac catttgttaa gtaagtcatc atgctacgat aaacgtcaga
      541 tgcaatctct agacttgatc cgtatactgg ggtcaagcgg tttttgtaac ctgtccaaac
      601 agccatggcg tagcgcttag taaagccaac aaagttttcg tctggcgcta atgtaccaac
      661 ataatctgga taaaggccgt atttttcacc aattttagct agttcctcat cagtgtagtt
      721 agaagtccct gttttaccag cttgcgcaac accaggaatg gcagcagcag tacctgtacc
      781 ataagtgaga acagttttta acatatctgt catcatatag gccgtggttt ctttcatggc
      841 acgttttcct ttttcatcaa aagttttact tgtaccatca ctaaattcca ctttattgac
      901 ataccttggt ttatgataaa taccaccatt tgcaaaagca gcgtatgcag cggccatttt
      961 ttcactactt gcaccatatt ttttatctga gctactgtta ttacttgaga tagcgtttga
     1021 gtagtgcatt tcgggatagt taatacctaa actgcttaag aaagatcgag catagtcaag
     1081 tcctgcggct tccagtgctc ggactgctgg gacatttcgc gatagcataa tagcagcttg
     1141 gattgtcatc catccgttat atctaaggtc ccagttaaac aattgggtag tggttccagg
     1201 ccaataatag actgagtcat tagtcgactg agcagtagaa gtataaacac cagattcaat
     1261 agcaggagca taggctgtga ttggcttcat ggtagaaccc cagtcacgat cagttaaaac
     1321 agcttggtta gtcccaaatg aaacattttc atcttgatta cgtccgccaa gctgagcaat
     1381 aacatgacca tttgtcacat caacaatcgt tgaagcaact tggaaatctt ggtctggata
     1441 gtaaacataa tcaccagaat gataaatatt ataaagagtc tgctgcgcat cggggataat
     1501 attggtataa acttttaaac cagcggtaaa aatatcttta ttcgtttctt ttttgacttc
     1561 ttcaataact tgttttagat aattatccat atattttgga taagttgagc gctgttggag
     1621 tgattgtaga ccttcagcga cgggagttgc gatggcagtt tcatattctg ctttcgtcag
     1681 atgtttttcc atgtacatct gttgcaacac gacgttacgg cgattttgag cagcttcagg
     1741 atgaaggtaa ggatcatatt gactaggagc ttgagggatt ccagccaata gggctagttg
     1801 ggcataagat aaatccttaa gatccttgcc ataataagac ttagcggctg tcagcatacc
     1861 atagttgcca ttacccatat atactttgtt gatgtaaaaa gtcaggattt cttgtttagt
     1921 atattttcgc tccatttgaa gagcaagcca aacttcttga gccttacgtt ttaaggtttg
     1981 atcagattca ttagtagaaa aataggctag tttaatgagt tgctgatcaa gcgttgaccc
     2041 cccttgagtg gtctgactcg ttagattatg aaaggcagca ccaaaaatac gataaagatc
     2101 tactccacga tggttaaaga aacgtttatc ttcaattgag gtaatagcat taactagatt
     2161 aatagggata ctatcagctg ttacattttc acgcttttca gaacccaaat cagcaatcag
     2221 attgttatta ccgtcataaa ccaagctaga gtttgttgat tttaactggg cttctgacag
     2281 tttcggagca ctgctaatgt agaaggtaaa caaaagacca ccaataataa taacaaggat
     2341 aataaggcta agaattgcac ttaatacata ctttagccac ttaaggattt ttggattttt
     2401 aattgtaatc accgcctaaa aaattttgtt caattatgtt taaataaggg acttgtggaa
     2461 aggctccaaa tgctacctta aagccatttt ttctgatata atcaatgggc atcgatttac
     2521 tgccattatc tatctgataa aatgaaatca aggcttgagc aggtaggtag taagtctcct
     2581 tcagtgtgga aaagtgaaga aggacaaagc agattccttt ttgatgtaaa acgcaagcca
     2641 tatgttcaat ctgatgtaaa tggaaatttt tcatgggcat agctgttttt tgcctggttt
     2701 cttttgcttc aaaatcaaca taatgtcctt tataaacgcc gcaatagtca gtcgttgaag
     2761 cttgtctaaa gtacgcttca acgatttttg cccgactgcg tttagggtaa tccactttga
     2821 caatttggac aggagtaggt tttttgtgaa tgacagctat ttgacgagat aaataataat
     2881 catttgtcgc gttgatagca gcttcaaagg acatacctct attggcgaag tccaccttat
     2941 tttgctttgt ttgcttttga acggaactta ctttttgtcg aataagatta tgaggataat
     3001 taaccattgt tctcctaata ttatctagtt aatgctaaaa atttaagcac ttttattata
     3061 acatattttt tgaaaggata acacctaatg actgcgattc taattacagg ttaccgaagc
     3121 tttgaaatag ggatttttga ccataaagac ccacgagttt caattattaa acaggctatt
     3181 cgtaaagatt taataggtta tttggaaaat ggtgtggatt ggtttatctt tacagggaac
     3241 ttgggttttg aacaatgggc tttggaagta gctaatgagc taaaagagga gtatcccttg
     3301 cagatagcta ctatattctt gtttgaaacg catggagata ggtggaatga aaaaaatcaa
     3361 gaagttttaa gtcagtttag ggctgttgac tttgtaaaat attactttcc aaattacgaa
     3421 caaccgacgc aatttagtca atattatcag tttttattag aaaagacaga aggggcttat
     3481 gttttttatg acacagaaaa cgagaccaac ttaaaatatt ttctcaagaa agcaaaagat
     3541 atgccgcatt atcagttgct attgctaacc tttgatcggc taaatgatat gtcacaaagc
     3601 tagcattgta tttttagggg tattggtatt taaatacttg ctttttgaac tatttttctg
     3661 tatcattaat aataatactg aaatataatt ttggtaaaaa gattgaccgt tcggagagta
     3721 aagaatgaca agtattattt atagtcctaa agatattttt gagcaagaat ttaagactag
     3781 tatgcgaggg tttgataaga aagaagttga tgaatttctt gataatgtaa ttaaagatta
     3841 tgaaaatttc aatgctcaaa ttgaagcttt aaaagctgag aacgaagcct taaaaaaggc
     3901 caagtttcaa gctagaaata cagtttcagc gactgttcaa caaccagtac cgcaaccgac
     3961 tcgtgttgct caatcagcta ctaattttga catcttgaaa cgtattagta aattggaaaa
     4021 agaggttttt ggtaaacaaa ttattgagta atgaaaactg tgcaattttt ggataatcgc
     4081 gtagtatttt aatactatga ggaaagtcca tgctagcact ggctgtgatg ccagtagtga
     4141 ttgtgctagg cgaacacata agcctaggga tgtgcataca cattacggcg aaggaaatgg
     4201 ctaagtcgtt tgatatgcca aagtacttct gaaagtgcca cagtgacgta gtttttatgg
     4261 aaacgtaaaa aatggaacgc ggtaaacccc tcaagctagc aacccaaact ttggtagggg
     4321 catgggatag ttggaaacga acaagctatc ctgactgtca acagacggta gacagatgat
     4381 tatcgaagga aataattcct agttatttcc ggaacaaaac atggcttata gaaaattgca
     4441 taataggtta agctagcact tgctagcttt tatttgattt aaaagaaaga gatatatgaa
     4501 agaaacattt agattagtcg caactgcagc cgcaggttta gaagctgtcg ttggtaaaga
     4561 agtcagagca ctaggttttg attgtcaggt tgaaaatggg aaagtttatt ttgaaggtga
     4621 tgtagaagcg attgttaaaa caaatctttg gttacgtgca gcagatcgta tcaaaattat
     4681 cgtaggacaa tttcctgcta gaacctttga agaattattc caaggtgtct ttgccttgga
     4741 ttggggaaat tatttaccgc taggagctaa gtttccaatc tcaaaagcca agtgtgtcaa
     4801 gtcaaagcta cacaatgagc caagtgttca ggcgattacc aaaaaagctg ttgttaagaa
     4861 actgcaaaaa catttccaca ggccagaagg agttcctctt caagaagttg ggtcaacgtt
     4921 taacattgag gtatctattt taaaagacca agccactatt atgattgaca cgacaggatc
     4981 tagcctattt aaacgagggt accgcgttca aaagggtggg gctcctatta aagaaaatat
     5041 ggcagctgct atcttggcct tatctaactg gtttccagat aaacctctgg ttgatcccac
     5101 ctgtggatcg ggaacgtttt gcatcgaggc agccctgatt gggatgaata ttgctccagg
     5161 atttaaccgt tcgtttgctt ttgaagaatg gagctgggta gataaagaca tggtgcaaca
     5221 agttcgtgat gatgccgaac aagaagctaa ttatgaaatc gaattagata tttctggctt
     5281 tgatatcgat ggccgcatga ttgagattgc taaaagtaat gctgaagaag ctggtttatc
     5341 tgatgtgatt acctttaagc aaatgcgtct gcaagacttt cgaacagaca aggttaatgg
     5401 tgtagtgata tcaaatcctc cttatggaga acggttgctt gatgacaaag cggttgacat
     5461 cttgtataat gaaatgggac aaacctttgc accattgaaa acatggagca aatttatcct
     5521 tacaagtgat gaactttttg aactaaaata cgggcaaaaa gctgataaaa aacgtaaact
     5581 ttataacggc accctaaaag ttgatttgta ccaattttat ggggaaaggg taaagcgaca
     5641 tctgatatag agaggtagta agcgtgtcag aagaaagcaa agaagtcgaa gtaacaaaag
     5701 aatcgcaaac attggggctt aatgaagcca agtcaatgac aattggtgaa gctgtacgca
     5761 aacagtcaga gataaaagca ggtgttacta aggatgatag tattttagat aagtacatta
     5821 agcaacaccg tgacgaagtt tcttctcaaa agtttgatgc taagtataca gagcttgata
     5881 cagctagttt agataacttt atcaaaaaac aaagagaagc attgagtaaa gccggtcttg
     5941 ttgatgacga acctgtgtct gcggagtctg ctgagcaaga tagcactttg gttgaagaag
     6001 tagctgaaga cttagctcca atggagacta ctgcggtggt aactgggatt ccggtggaag
     6061 cgacagtccc tgtgttagat ctagatccat cagagagggg cattccagaa ccacagatga
     6121 ccaaggaaga gccaaaaaga gatcagtttc tttctgaaga tagccaccat ccagccaaac
     6181 aaaacacgaa aaaaggttgg ttaatagctt tatttctact attattggcc attcttgcag
     6241 ttgtatttgg ttggaaccat ttcttgagac aagacagtgg taaaacaact caaacagcaa
     6301 gtaaacagac aaagacctct ttgcagaccg acagtgctaa aaaagcaacg cgcttgaagg
     6361 ctgctgctaa agctttcgaa aaacaatatg gtacttttta tacagatgcc acaaagagca
     6421 aattaaaaaa tagtgccttt gctactttgc cagatttaga agctgccttg aaagctttgg
     6481 aaggctctgc ttactatgat aaggcgaaag caaaagtcga ttcccttaag aaagctattg
     6541 ctgctattac agcggttaac ggtaaatttg tttccgatgt tgttgtagat ggtgaaaagg
     6601 tttcggctga agtcaaggct gatgctaatt ttgatgattt atcaagtgca accttgacaa
     6661 caggaaatgc aaatcttgat gctgtcttgc aggcaagcat cacagaaggt cgtcagcaac
     6721 tagccagcaa ggctgaggcc gctaaagcag ctaatgagca agcagcccag gctcaagcag
     6781 ctcaaggaca aagtacatca gtagcaccat ctggctatgg cttgacaagt tatgaccctg
     6841 caagcttaca acggcattta tcacgtgtac cttacaatca agatgttatt gctgacagag
     6901 ctaacccatc ttgggcattt aatccaggag ttcttgaaaa aattgtagca acctcacaag
     6961 ctagaggtta tatttcaggc taccaatata ttttagagcc ggttaatatt attaatggca
     7021 atggttacta taatatgttt aagcctgatg gcacttatct gttctctatt aattgtaaga
     7081 cgggttattt tgtaggaaat ggcaaaggtt atgctgatgc tttagattac tgagaaaatc
     7141 aaaaaaaact gagataaggc aacttgtctc agttttttga ttaataaagt atgttgtctt
     7201 aaaatctgca gatagacttg ttttttgtaa caagtcaaat gggtcagtag gattgttatg
     7261 tttgacaaca cactagtatc agatgacatg gcgactaaaa ggatcgtctg aaatcccttg
     7321 atcaataatc aattgagccc attctttggc ggcaaagagg ctatgatcct tatagtttcc
     7381 acaggattca agagttgttc caggaacatc ttcccaagta atcccagttg caatttcttc
     7441 taggctggat ttaatcacct tggcaatatc agtagaactg tgttttcccc acatgataag
     7501 gtgaaaacct gttcgacagc caaaaggaga gcaatcaatc atcccatcaa tgcgttggcg
     7561 gatgagcttg gcaagtaagt gttcaatggt atgcaaaccg gctgtttcaa tagaattttg
     7621 gttaggctgc actaggcgaa catcaaaatt tgtaatacga tcgcccttgg gtccaaattc
     7681 ttcagaaata agacgaacat aaggggcctt tacaatagta tgatctagct caaaactttc
     7741 gacgataact tcttttgtca tttcaatctc ctttgtttta cactttatca taacacaaat
     7801 agtgagaaag tggctacttg tggaggagaa aaggctaaga aaacagatca aaggctatca
     7861 taatgacctg tgcgataaaa gtacttacag aaacagtaat aaaggtttct ttaaaagcat
     7921 ttttgtggtt aagtcctgtc aatgctaaga aggtgagaaa gacaccactt tggggcacaa
     7981 tggtaaagat attagaagca atagtggcaa cacggtgaat catttcggga ttaaggcctt
     8041 gatctaaata atactgggca aagttaggca tgacaattcc tagagcaccg gaagaagagc
     8101 cggtaatagc tgacatagag gaagtcaaca cggctaaact aataagagga ttaccgggaa
     8161 tattcaaaat gagatctgag aagaatgtga aacctggggc aatcatcaca acggctccga
     8221 aggcaactgc agatgctgtc gcaaaaatag gtgcaataga gctgctagct cctaaattaa
     8281 agacagcaat cttattagga ataaactgtc taaagagcca gcttgcggtg agaatggcag
     8341 ctaaaagtgc tatgaaaata atgttcttct taaaaaaatc gtttccgaaa aggctaccgg
     8401 ttagggcgat aataatgagc aacaataaag gtaagatact ggcaaggaaa tgtgggagat
     8461 tttttgtttt cacttgtatc tcattgtcaa aagcataagt ggcataagtt tcaccccttg
     8521 ccatactttt tgctagacaa tatttcatat aaaagagccc aaaagtcacg cagccaattg
     8581 agccaacgat actcggaatg gcggcagcag ttagtgaggt gtccaagtat tgaataggaa
     8641 taacattttg aatggcaggt gttccgggta aaatcgtcat ggtaaaagtt gcaataccga
     8701 gccaaagagg aacttgaatt aagttccaag ccaagtccat ttttttgaat agacttcgtg
     8761 caagcggcaa gacagcaaac atgacaacaa agaggctgat tcccccgtaa gttaatatag
     8821 cactgattag aaaaatagca acgagtactt tataaggact atcatgacca acttttttta
     8881 aaatataatc tgcaatagaa gtggtttcac cgcttgtttc cattaatttg gctaagatag
     8941 aacctaataa aaagatagca aaataattga gaatataagt ggagagtgcc cccataaatt
     9001 ggttgggttc ttttccaagt aaggttgtgg ttggatccat ttgattaaac aaaatgacca
     9061 agctggtagc aagaggcgca gcgatgataa tgttaacttc tttgacagac aggtaaataa
     9121 taacaataac acctatcaag acccccagag aaccaataat ttccatagtt ttatgacctc
     9181 cttattagaa aataaagaga cagatcacaa gatacagcct ctttatctct ccaaatttat
     9241 tgagcagtgt agccaccgtc taagatacag gcttgacctg taacaccttt tgccttatca
     9301 ctggcaagga aagacacata gtctgcaatt tcttgaacgt caatgaggcg tttttgagga
     9361 acaagtgggt atagcacttc ttcaagaaca ttttcaaggg gaatacctct tgttttggaa
     9421 aggtcttcaa actggccacg aaccagtggt gtgtcaacat atccaggaca aatggcattg
     9481 accgtaatgc ctgaatcagc agcttctaag gcagttactt tggtcagacc gatcaagccg
     9541 tgcttagctg aattgtaggc ggatttgcca gcaaaaccaa tgacaccatt gatagaagcc
     9601 atattaataa tacgaccgtg tttttgagct ttcattgtag gaaaagcacg tttaatggca
     9661 ataaatggtg ctgtcaacat gattttaatc atgaattcaa atttttcagt tggaaaatct
     9721 tcaatcatgg caacatgttg aaggcctgcg ttgttaacca aaatatcaat acgaccatat
     9781 ttttcaacaa ccgtatcaat agcagcattg atggcttctt ctttggtgac atcgcacaca
     9841 acactaaaag cgtcatagcc ttctttagta tagtcagcaa caacctgatc cagtttttct
     9901 tgattaatgt cagaaaaaac aaccgtttta ccttctttta agaaggtttc cccgatttgt
     9961 tttccaatcc cgcttgcagc accagtgata aatgcaactt ctttagtatg tgtcatgata
    10021 ctatcctcgt ctttcttttg gttagtcttt agtttgtttt atcaattagg gtaacttcgg
    10081 taacgttttg cacgtcttca aaggtgtaat ctgggttaat ttcaagggcg catagacctt
    10141 catcttgata ttcaaagact cccatttcag taatgattag gttaacaaca ttttgagcag
    10201 tgagaggaag ggtgcatttg tctaaaattt ttgctttgcc tttattggta tgctccattg
    10261 caacaatgac ttttttagca cctaccaata agtccatggc ccctcccatc ccaggaacca
    10321 tttttccagg aatcaggtaa ttagcaatgc tagcttcttt atcaacttgt aaggcaccta
    10381 agacggttgc ggccacatgg ccgcctcgaa taataccaaa ggaagttgag ctatcgaaga
    10441 aagcccctcc aggagcaata ctaacaggtt gcccaccagc attaacaata ctaggatcgt
    10501 agtgatcatc tgttaatcca gtgagaccga caaaaccatt ttcggactgc agggtaatgg
    10561 ttacaccttc aggaatatag ttggctactt ttgttggcaa acctatgcct aagttgacca
    10621 gtgtattgtg ttctaattct tgtgcaacgc gcttagcaat acgtgtttga atctcttctt
    10681 ttgaaagaac agtagctgat gttcccatgt taggctcctt tcactaagta gttaacaaaa
    10741 atgccaggag tatggacaaa attaggatcc atttgcccca cgtcaacaat ttctcttgct
    10801 tcaacaatgg tggttttcgc attagcagcc atcacatgat taaagttatt ttcagaacct
    10861 gcgtattgca aattgccatt tttatctgcc ttgttagcaa aaataagtgc gatatcagct
    10921 ttcaaaggtt tttcaagaag gtagtctttg ccgtcaacag taatgacttc ttttcctttg
    10981 gcaacttcgg ttcctattcc agtaggtgtt aagaaaccgc ctagcccaaa accgccactg
    11041 cggatgcgct cagctaatgt tccctgaggt acaagatcaa ttacagtctc gccctcagtc
    11101 atttgtcggc ccgcttcacg gttgagaccg atatgtgatg caataatagt tgaaaactgt
    11161 ttattggcaa ccatcttacc aacgcctttg tcaggaaaac cggcatcgtt acagattaaa
    11221 gtgaggtctt tgacaccttt ttccacaagt gtatcaatta atttttctgg tgtgccattt
    11281 gtcatgaagc caccaaccat aatggtatca ccatctttaa cgtgagctac cgcttctttt
    11341 attgtgattt ctttacacat tttttctacc aactttcacg agactaagta ttgtttttca
    11401 cgataacagc agttccttgt cctccaccaa tgcaaagtgt tgctagtcct ctggttactt
    11461 ggcgtttttt catttcgtgg attaatgtga ctagaatgcg agcgccactg gcaccaatag
    11521 gatgacctaa agcgatggct cctccattaa cattcacgat ttctgggttt aactttaaat
    11581 cttttaaaac cgccaaggac tgtgctgcaa atgcttcatt ggactccact aagtccaaat
    11641 cagaaattgt taatccagct ttcttaaggg ctttttgagt ggcaggaatt ggtccagttc
    11701 ccatcagttc aggtgcaaca cctgcactag catagctttc aatagtgact aaaggtgtta
    11761 accctaattc aagagctttt tcttcagtca ttaacatcaa taaggctgca ccatcgttta
    11821 ttcctgaggc attcccagca gtaacggttc cttctttagg gaggaaagca ggacgtaatt
    11881 gctgtaattt ttcaaggctt gtatcaactt ttgggtattc atctgttgta acaattaaag
    11941 ggtctttgcg tcgttgaggc acagagacag ggacaatttc gtcagcaaaa cgatgatttt
    12001 ctaatgcttt agcagctttt tcttggcttt ctaaagcaaa ttgatcttgc tcttctcttg
    12061 agatgccata tttctgagca acgttttcag cagtaatacc catgtggtaa ttgttaaaag
    12121 catctgttaa gccatcatga acaagagtat caatcaattg actattacca agacgttgac
    12181 caaagcggtg ctgagttgag acataagctg cttgtgacat gttttcgaca ccaccagcaa
    12241 taacgatatc agcgtcacca agcatgatgc tttgcgcagc taattggatt gctttaagtc
    12301 cagaaccgca aaccatatct aatgtgaaag ctgttttatc ttcaggaatt cctgagtgga
    12361 ctgcaacttg tcttgccaca ttttgtccta ggcctgcatg aaggacgttt cctaggatga
    12421 cttcatcaac catttcaggc tttacttgcg ttttatcaag gatttttttc agtacagttg
    12481 ttccaagttc aacagcagag acacttttaa aggagcctcc aaaagctccg ataggagttc
    12541 gtaaagcttc aacgataact atgtttttca taaaataaac atcctttcag ttttagaaag
    12601 cgctttccat gtctctaaga taacaagaaa acgctaaaaa taaaaatata gaatttttat
    12661 aagcatataa ttttttttga tagagttggc aatagcattt aacatctact tgtgtcataa
    12721 tataggtaat tataccggaa aaagtaattg ataagacaag gagaatccta tttggatatt
    12781 aaacaaattc gttattttat cgcaatcgtt gaaaatcatt ttaacttgag ccaggcggcg
    12841 gaactgttgt atgtttctca accgacgctg agtatgatga ttaacgattt tgaaaaaaga
    12901 gaaaatgtta agctcttcaa acgaaaaaga ggtcgtatta ttgggttaac ctatcttggt
    12961 gataattatt ataaggacgc tcaaaaggtt ctcagtctct atgatgacat gtttttgaaa
    13021 ttgcatgatc acagtaaagg gttaaaggga agtatcagta ttggtattcc tcctcttatt
    13081 ttatcagttg ttttctcaga agtaatgcca aaactgattt tagaaaatcc ggggattcaa
    13141 ttcaatgtta aggagattgg tgcttaccaa ctaaaaaatg aactactcgt gggaaatgtt
    13201 gacgtcgcag tcttgttatc cccaacaggc atcgctgata atttggttga gacttatgaa
    13261 atccagagat cagaattatc tgtatgcctg tctccacgcc atcgtttagc ttccaaaaaa
    13321 gttattcagt gggaagattt aacagatgaa caacttgccc tatttgatcc tagtttcatg
    13381 gttcaccatt tagtgcttga agcttgcgag cgtcaccaag tcagacctaa tattattttg
    13441 acgtcctctt cctgggattt tatgcttaat tctaccaaga ttaatcacaa cgtcttaact
    13501 atttgtccca aacctattac agaattatac caattcaagg acattaagtg tattctaatg
    13561 gaacgtccca tttcttggcg tgttgtctta acacgcctcc ggaaaaaaag ttattctgaa
    13621 atagaagcct acatcatgga cgacttgcta cagtcttttt ttaagtaggg ttctcaacta
    13681 cttggcccaa atgttaattt ttttaaaaat gaattggaat tgtgtaaacg ctcaatttat
    13741 gataaactaa gagatggctt tctattaatg aaatctagaa tatattgagg tacaaacatg
    13801 gttaatatta ttttattaat tgtttctgcc ctcattggtt taatattagg ttatgcactt
    13861 atttcgatta gactcaagtc tgcgaaggaa gctgcagagt tgactctttt aaacgctgaa
    13921 caagaagctg ttgatattcg tggcaaagca gaagtagatg ctgaacacat caaaaaaaca
    13981 gctaaacgtg aaagtaaagc aaatcgtaaa gaattacttt tagaagcaaa agaagaggca
    14041 agaaaatatc gtgaagagat tgaacaagaa tttaagtctg aaagacaaga gcttaaacaa
    14101 ctcgagacac gcttagcgga gcgctcctta actcttgacc gtaaagatga aaacctatca
    14161 agtaaagaaa aggtactaga tagtaaagaa caaagtctga ccgataaatc taaacacatt
    14221 gatgagcggc aacttcaagt ggaaaaactt gaagaggaga aaaaagcaga actggaaaaa
    14281 gttgctgcga tgacgattgc agaagcgcgt gaagtgattt taatggagac ggaaaacaaa
    14341 ctgacccatg aaattgcgac gcgcattcga gatgccgaac gtgacatcaa ggaccgaaca
    14401 gttaaaacag ccaaggactt gttagcgcaa gccatgcaac gccttgctgg tgagtatgtg
    14461 actgaacaaa ctattaccag tgtccatctc ccagacgaca acatgaaggg ccgaattatt
    14521 ggacgtgaag gccgtaatat tcgtacttta gagagcttga ctggcattga cgttattatt
    14581 gacgatactc ctgaagttgt tatcttatca ggatttgatc ctattcgacg tgaaattgct
    14641 cgtatgacct tggaatctct gattgctgat ggtcgcatcc atccagctcg tatcgaggaa
    14701 ttggttgaga aaaatcgtct tgaaatggat aatcgtattc gtgagtacgg tgaagctgca
    14761 gcctatgaga ttggtgcacc aaaccttcat cctgatttga ttaaaatcat gggacgcctg
    14821 caattccgta cctcgtttgg tcaaaatgtc ctacgtcact ctgttgaggt tggtaagtta
    14881 gctggtattt tagctggtga actgggtgaa aatgttgccc ttgcccgccg cgctggtttc
    14941 ttgcatgata tgggtaaagc tattgaccgt gaggttgaag gtagtcacgt tgagattgga
    15001 atggaatttg cacgtaaata caaagaacat ccagttgttg tcaacactat tgctagccac
    15061 cacggagatg tggagccaga ttctgtgatc gctgtgctag tagctgcagc agacgctctc
    15121 agttcggctc gcccaggcgc tcgtaatgag tcaatggaga attacatcaa gcgtcttcgt
    15181 gatttagaag aaatcgcgac aagttttgat ggggtacaaa atagttttgc tctacaagct
    15241 ggacgtgaga ttcgtattat ggttcaacct gaaaaaattt cagatgatca ggttgtcatt
    15301 ttgtcgcata aagtaagaga aaaaattgaa aacaatctag attacccagg aaatattaaa
    15361 gtaactgtta ttcgtgagat gagagcggtt gattatgcca agtagaaaaa gaggaattat
    15421 cctctttttc tttatgactt gcctttgaaa atgttcttga aaaagactag cctttttgat
    15481 aaactattaa aaagcaacta ataaggagaa tataatgtct gaacgcggtt tgttaatcgt
    15541 cttttctggc ccttcaggtg tcggaaaagg aacggtaaga caagaaattt tttcaacgcc
    15601 agatcataaa tttgaatatt cggtttcaat gacgactcgt ccgcaaagac cgggcgaggt
    15661 tgatggggtt gattatttct tccgcacacg tgaagaattt gaagaactca ttaaaactgg
    15721 tcaaatgcta gagtatgctg aatatgttgg taattactat ggcacccctt taacttatgt
    15781 taatgagact cttgataagg gaattgatgt ctttttagaa attgaagttc aaggagctct
    15841 tcaggttaag tcaaaagtac cagatggtgt ttttgttttt ttaacaccac cagatttaga
    15901 tgaattagaa gaccgcctgg ttggtcgtgg tacagatagt caagaagtca tagctcaacg
    15961 tattgaaaga gcaaaagaag aaattgccct tatgcgcgaa tacgattatg cagttgttaa
    16021 tgatgaagtt gccttggcag ctgagcgggt taagcgtatc attgagacag aacactttcg
    16081 tgtggagcgc gtgattggtc gttatgataa aatgattaaa ataacaaaaa atccctttaa
    16141 agctaaatag aaaagagtaa gcattatgat gttaaaacct tccattgata ccttattaga
    16201 taaagtacca tctaagtatt cacttgtgat tttacaagcc aaacgtgccc atgaactaga
    16261 agctggtgcc actccaactc aagaatttaa atcagtgaag tcaacacttc aggcacttga
    16321 agaaattgaa tcaggaaacg ttgtgattca cccagatcct tcagctaaac gtgaagctgt
    16381 ccgtgctaag attgaggcag aacgcttggc caaagaggaa gaggaacgta aaatcaaaga
    16441 acaaattgcc aaagaaaaag aagaagaagg agaaaaaatc taaggtttca gaaatcttag
    16501 attttttcat atttagtacg atgtccatga cagaaaagga gaagcaaatg gaaaaagtag
    16561 ctcatgtgat tgtagatatt ccattgatgc aaacggacaa gccattttct tatggtattc
    16621 caaaagagtt agtttctctc gtgcagcttg gttctcgtgt gcatgttcct tttggaaaag
    16681 ggaatcgttt gttgcaaggc tttgttattg gttttggcca ggaagattca agctctctta
    16741 aactgattca gaccgtttta gatcctgagc cagtcctaaa ccaagaacaa ttgacccttg
    16801 ctgatcagct tcgaaaaaca gttttttctt ataaaatcac gctcttaaaa gcaatgattc
    16861 ctaatcttct taactctaac tatgataaag tgctaaggcc agagtcaggt ctaaaaaagc
    16921 ctgataggga ctttcttttt gaaggaaaac catctgtctt atattctacc ttagaccgcg
    16981 agaaggaaaa aatagctctt aagggcatac aagctgggca tattactgtt agttatttag
    17041 ccaaagacaa gaaaaaccta aagacagaaa aatattatca cgtcgactta gacgccttag
    17101 cggtacatcc catctctagt cgtgccaaaa agcgtcaatt attaaaagac tatctgctaa
    17161 cccatacgaa ggaagctaaa cttgcaacgc tttatcaagc cttttctaga gatgtggtgg
    17221 cttactttgt gacaaatcac ttaattcgta ttgacgaacg tcaaattgat cgcagtgagc
    17281 cctattttga ccagatcaaa ccatcttctt ttttaacctt aaatgaacag caggcatcag
    17341 ctgttactga aattgttgag caaattggga aaccgagtaa accttttttg attgaaggta
    17401 ttacaggttc gggtaagaca gaagtttatc ttcatatcat cgaagcggtt ttaaaacagg
    17461 ataagacagc cattgtttta gtaccagaaa tttcgttgac tcctcagatg acctctcgtt
    17521 ttatttcccg tttcggaaag caagtggcta ttatgcattc aggcttatca gatggtgaaa
    17581 agtttgatga gtggcgcaag ataaaaacag gacaggctaa ggttgtggtt ggtgcccgct
    17641 ctgctatttt ctctccacta gagcggattg gtgcaattat tattgatgag gagcatgaat
    17701 caacctataa acaagaaagc aaccctagat accatgccag agaggttgcc ctgctcagag
    17761 ctaaacacca tcaagcagtt gtggttatgg gctctgctac gccaagtatt gaaagccgag
    17821 caagggctag taagggtgtc tatcatttta ttcaattaac gaaaagagca aaccctcttg
    17881 ctaagattcc agaagtaacg attgttgatt ttcgtgatta tattggacag caagcagtta
    17941 gtaattttac cccatatttg attgataaga ttaaagaacg tttggttaaa aaagaacaag
    18001 ttgtcttaat gttaaataga cgtggttatt ctagttttgt gatgtgtcgg gactgtggtt
    18061 atgtggacaa atgtcccaac tgtgatatct ctttaaccct tcacatggat actaaaacta
    18121 tgaattgcca ttactgtggg tttcaaaaac ctatcccaat tacttgtcct gagtgtcata
    18181 gcaacagtat ccgctattat ggcactggaa ctcaaaaagc ttttgatgaa ttgcaagggg
    18241 ttatcccaga agctaggatc ttgagaatgg atgttgacac cacacgaaaa aaaagaagtc
    18301 acaaaaccat actagatagt tttggtcgcc aggaagcaga tattttgtta gggacgcaaa
    18361 tgattgccaa aggactggat tttccaaatg tgactttagt tggggtacta aatgcagata
    18421 cctccttaaa tttgccagat tttcgagcgt cagaaaaaac gtttcaattg ttaacacagg
    18481 ttgctggtcg ggcaggccgt gcccataagc caggggaagt cttgattcaa acttataatc
    18541 ccgatcacta tgctattcaa ttagctaaac aacaagattt tgaagccttt taccgttatg
    18601 aaatggcgat tcgccgtcag atggcttatc ccccttacta ctttacagtg ggtattacgc
    18661 tttcgcatcg tttagaagca tcggttgtaa aaaaagctta tcaagttaca gaactgctaa
    18721 agagtcactt atcggataat attaaaatat taggaccgac tcctaagcca attgctagaa
    18781 cacacaattt ataccactat caaattttgt taaaataccg ttttgaagat aacctagaag
    18841 agacgctcaa tcgtattctt gattggagtc aagaagcaga taatcgtcat ttaaaactaa
    18901 ttattgattg tgaaccgcaa caatttttat aaggtaaatc actaaatcta ggatgatcag
    18961 ttatgatata ataaaaatac aaagaggaaa gtcatgataa aattattatt tatgggaact
    19021 ccccaatttt cagcaactgt attgaagggc ttgctagata atcctgccta tgagatttta
    19081 ggagtggtta ctcagcctga tcgagctgtt ggacgcaaaa aagatattaa ggtaacacct
    19141 gtaaagcaat tagctcttga gcatggaata tcaatctatc agccggaaaa attatcaggc
    19201 tctcaagaac tcattgagat tatggaactt ggtgcagatg gtattattac agcagcattt
    19261 ggccaatttt taccaacaat attacttgat tcagtttcat ttgctattaa tgtacatgca
    19321 tcattgttac ctaaatatcg aggtggagca cctatccatt atgctattat gaatggagat
    19381 aaagaggctg gcgttactat tatggaaatg ataaaagaaa tggatgcagg tgacatggtc
    19441 gctaaagcaa gcactccaat tcttgaaacc gataatgtag gtactctttt tgagaaatta
    19501 gccatcattg gtcgagactt acttttggat agtttgccag cttatctttc tggagaatta
    19561 aaacctattc ctcaagatca cagccaagct actttttccc ctaatatctc tccagaacaa
    19621 gaaaaattag attggacaat gtctgatcaa gaagtgttca atcatattcg aggtatgaac
    19681 ccttggccgg tagcgcatac gtttttagag gggcaacgtt taaaaattta cgaagctcaa
    19741 ttagctgaag gagaaggatt acctggacaa gttattgtca aaaccaaaaa atctttggta
    19801 atagctgctg gtcaaggagc cctgtcattg ctggttgtgc aacctgcggg gaaacctaaa
    19861 atggctattg cagatttctt aaatggcctt ggtagaaaac ttgaagtggg tgatttcatt
    19921 ggcagataat tggaaaataa gtattcgagg aaaagcactt ctaaccttag aacatatttt
    19981 tgatcagggg gcttacacaa acatagcgct taatcagcag ctaagcaata aagctttatc
    20041 tgctaaggac agagctctat taactgagat tgtatatggt accgtttcac gtaaaattag
    20101 cttggaatgg tatttagccc attatgtcaa agaccgagat aaattggaca aatgggttta
    20161 ttatctcctt atgctcagtc tttatcaact gatttattta gataagttac cagcgcatgc
    20221 cattgttaac gatgcggttg gtattgccaa aaatcgtggc aataaaaaag gtgcagagaa
    20281 gtttgtcaat gctattttac gtcaattcac cagccatcct ctcccagata tggaaacaat
    20341 taagcgtcga aataagtatt attcggtcaa gtattcactg cctgtttggt tggttaaaaa
    20401 gttagaagac caatttggtt ctgacagatc agtggccatc atggaaagtt tatttgtgcg
    20461 tagtaaggct agtattcgcg tgacagaccc tttgaagcta gaggaagctg ctgaggcttt
    20521 agatgcagaa cgttcccttt tatcagcaac tggtttaacc aaagcgtcag gtcattttgc
    20581 agccagtgat tatttcacaa atggggacat cactattcaa gatgaaagca gtcagcttgt
    20641 agctcctact ttaaacattg acggtgacga cataattctt gatgcatgta gcgctccagg
    20701 gggcaagacc tctcatatag catcttactt aaaaacaggt aaagttattg ccttagatct
    20761 ctatgatcat aaactagagt tggtgaagga aaatgctaac cgcttaggag ttgctgataa
    20821 cattgagaca agaaaactag atgccagaga ggttcatcgt cattttgaaa aggatagctt
    20881 tgataagatt ttggtggatg ctccttgctc aggaattggt ttgattcgtc gaaaacccga
    20941 tatcaaatat aataaagaaa gtcaagattt caatgctcta caggctatcc agctagagat
    21001 attgtctagt gtttgtcaaa ccttgcgtaa aggtggtata ataacataca gtacttgcac
    21061 tatttttgat gaggagaatc gtcaagtcat cgaagcattt ttacaaagtc atcctaattt
    21121 tgaacaagta aaactgaacc atacacaagc agatattgtc aaggatggtt atcttattat
    21181 cacaccagaa caataccaga ctgatggctt ctttattgga caagtcagac gagtcttgta
    21241 gtattttagg aggaaactga ctgagtcaga aaaaacgtat gaagatttca ttaaaaacag
    21301 acattgggca aaaacgctca aacaatcaag attttatcaa taagtttgat aataaaaaag
    21361 gcatcacttt agtgatttta gcagacggta tgggtggcca cagagctgga aatattgcca
    21421 gtgagatgac agtcacagac ctgggaagag aatgggtaaa aacagacttc acagagttaa
    21481 gtcagattag agattggtta tttgaaacta tccagtcaga aaatcaacgg atttatgatc
    21541 taggccaatc agaagacttt aaaggaatgg gaacgactgt tgaagcagta gctttagttg
    21601 aatctagcgc catttatgct catattggtg attcacggat tggtcttgtc catgatgggc
    21661 attatacctt attgaccagt gatcattctt tagtgaatga gttggttaag gcaggacaga
    21721 tcacagaaga agaagctgct agccatccac aaagaaatat tatcactcaa tctattggtc
    21781 aagcttcccc agtagagcct gatttaggag tgagggtttt ggagccaggg gattatcttg
    21841 tcattaacag tgatggtcta accaatatga tttcaaatga tgaaattgtg actatcttgg
    21901 gcagtaaggt tagtcttgat gaaaaaaacc aagaaatgat tgacttagct aacctaagag
    21961 gaggcctaga taatattaca atcgctttgg ttcacaacga aagtgaggac gtcgaatgat
    22021 tcagattggc aaattatttg ctggtcgtta tcgcattctg aaatctattg gccgcggtgg
    22081 tatggcggat gtttatttag caaatgattt gatcttggat aatgaagacg ttgcaatcaa
    22141 ggtcttgcgt accaattatc aaacagatca ggtagcagtt gcgcgtttcc aacgagaagc
    22201 gcgggccatg gctgaattga accatcccaa tattgttgcc atccgggata taggtgaaga
    22261 agacggacag caatttttag taatggaata tgtggatggt gctgacctaa agagatacat
    22321 tcaaaatcat gctccattat ctaataatga agtggttaga attatggaag aagtcctttc
    22381 tgctatgact ttagcccacc aaaaaggaat tgtacacaga gatttaaaac ctcaaaatat
    22441 cctactaact aaggagggtg ttgtcaaagt aactgatttc ggcatcgcag tagcctttgc
    22501 agaaacaagc ttgacacaaa ctaattcgat gttaggcagt gttcattact tgtctccaga
    22561 acaggctcgc ggctccaaag cgacgattca aagtgatatt tatgcgatgg ggattatgct
    22621 ctttgagatg ttgacaggcc atatccctta tgatggcgat agtgctgtta cgattgcctt
    22681 gcaacatttt caaaagcctc ttccatctat tatcgaggag aaccacaatg tgccacaagc
    22741 tttggagaat gttgttattc gagcaacagc caagaaatta agtgatcgtt acgggtcaac
    22801 ctttgaaatg agtcgtgact taatgacggc gcttagttat aatcgtagtc gggagcgtaa
    22861 gattgtcttt gaggatgttg aaagtaccaa acccctcccc aaagtggcct caggtcccac
    22921 cgcttctgta aaattgtctc cccctacccc aacagtgtta acacaggaaa gtcgattgga
    22981 tcaaactaat caaacagatg ctttacagcc ccccaccaaa aagaaaaaaa gtggtcgttt
    23041 tttaggtact ttattcaaaa ttcttttttc tttctttatt gtaggtgtag cactctttac
    23101 ttatcttata ctaactaaac caacttctgt gaaagttcct aatgtagcag gcactagtct
    23161 taaagttgcc aaacaagaac tgtatgatgt tgggctaaaa gtgggtaaaa tcaggcaaat
    23221 tgagagtgat acggttgctg agggaaatgt agttagaaca gatcctaaag caggaacagc
    23281 taagaggcaa ggctcaagca ttacgcttta tgtgtcaatt ggaaacaaag gttttgacat
    23341 ggaaaactac aaaggactag attatcaaga agctatgaat agtttgatag aaacttatgg
    23401 tgttccaaaa tcaaaaatca aaattgagcg cattgtaact aatgaatatc ctgaaaatac
    23461 agtcatcagt caatcgccaa gtgcgggtga taaatttaat ccaaacggaa agtctaaaat
    23521 tacgctcagt gttgctgtta gtgatacgat cactatgcct atggtaacag aatatagtta
    23581 tgcagatgca gtcaatacct taacagcttt aggtatagat gcatctagaa taaaagctta
    23641 tgtgccaagc tctagctcag caacgggctt tgtgccaatt cattctccta gttctaaagc
    23701 tattgtcagt ggtcaatctc cttactatgg aacgtctttg agtctgtctg ataaaggaga
    23761 gattagtctt tacctttatc cagaagaaac acactcttct agtagctcat cgagttcaac
    23821 gtcaagttca aacagttctt caacaaatga tagtactgca ccaggtagca acactgaatt
    23881 aagcccatca gaaactactg ctcaaacacc ttaacaaaga atagttagtc tcatgtttta
    23941 ctagcatgcc actaactatt ctttttaact actcgtcata taaagatgca attttccagg
    24001 aattctgata aaatacaaag aaataagggg aaactaatga aaaaatttca attcttttta
    24061 cttatcgagt gtatactgct agctatgggt atcatgacaa tcttggataa tgatttatca
    24121 agttttatcc taattctagt tcttatctta ttagcattac gcttttataa ccaagatagc
    24181 cgcaataatt ttttactaac tgtcagttta ttgtttcttt ttttaatttt catgcttaat
    24241 ccctacatta tcatggctgt tcttttgggg atagtttaca ttttcattaa ccatttttca
    24301 caagtcaaaa agaagaatcg ctttgccttg attcgtttca aggaagaaaa gattgaagtg
    24361 aacaatacca agcatcaatg gattggtact gctaattatg aaagtgatta ttattgtttt
    24421 gatgacatta acatcattcg aatctcagga aatgacacgg ttgacttaac caatgttatt
    24481 gtgacaggaa tggataatat cattgtgata cgtaaaattt ttggaaatac gactatattg
    24541 gtacctattg atgtcactgt tactttagat gttagttcca tatacggaag tgttgatttt
    24601 tttagatgtc agcaatatga tttgcgcaac gaatctatta agttaaaaga aacggataac
    24661 caatccctta aaaaagtcaa aattatcgtt acaactattg ccggaaatgt tgaggtggat
    24721 aggcgatgaa aaaacgttac tatgctcttg tttggctcta ctcaaccatt accattttat
    24781 ctattgtttt tgtggtcatg gataatttag gaatcacttt taactacctt cgcaatcatt
    24841 tatggcaggt cgaacgtcta gggttttcga ttttattgtt gattgtttct gtgactttat
    24901 tattgttgtt attgtggatt attatggatg ataacagtaa gcgtaacatc aaccaaaatc
    24961 taaaatatat tctcaataat cgacgactct atctagatga gacatcagaa atcaatacta
    25021 atttaagtcg actttctaaa aaaatgtctc acctgactgc taacatgcaa aagaaagaaa
    25081 gtgcttatat tcttgatagc caagaagttg taaaacaaga gcgcaaacga attgcaagag
    25141 atttacatga cacagtcagt caagagctat tcgcttcgtc attgatttta tcaggaattt
    25201 caatgagttt ggaacaactg gacaaaacac aattacaaac acagttaaca acggttgaag
    25261 caatgttgca aaatgctcaa aatgatctac gcattctcct tttgcatctt agacctaccg
    25321 agctagctaa tcggacttta tctgagggcc ttcatatgat tcttaaggaa ttaacagata
    25381 aaagtgatat tgaagtcatt tataaggaaa ccattgctca gcttcctaaa acaatggaag
    25441 ataatctttt tagaattgcc caagaattca ttagcaacac gttaaaacat gctaaagcta
    25501 gtcgaattga agtttatctc aatcaaacct caacagaatt acaattgaag atgattgatg
    25561 atggtgtagg atttgatatg gatcaggtaa gggatttgag ttatggtctg aagaatattg
    25621 aagaccgtgt caatgattta gcaggaaacc tacatttaat tagtcaaaaa ggcaaaggag
    25681 tttccatgga tattagactg ccgatagtga agggagatga cgatgagtaa gataaaagtg
    25741 atattggtcg atgatcatga aatggtccgc atgggactca agagtttttt gaatttacaa
    25801 gctgatattg atgtcgttgg tgaggcctct aatggacgtg aaggggttga tttggcattg
    25861 gctttgaagc cagatgtttt ggttatggat ctagtgatgc cagagttagg cggtgttgag
    25921 gcaactttag aagttctaaa aaaatggaaa gaggctaagg tacttgtgtt aacttcctat
    25981 ctagataatg aaaagatata ccctgtcatt gatgcaggag cgaagggtta tatgttaaaa
    26041 acatcgagtg cggctgaaat tttaaatgcc attcgcaagg tttcaaaggg agagttagct
    26101 attgaaacag aagttgacaa aaaaattaag gcgcatgatc aacaccctga cttgcatgag
    26161 gaactaacag cgcgtgagta tgatatttta caccttttag ctaaagggta tgataatcag
    26221 accatcgctg atgaactctt tatttcctta aaaaccgtca aaacacatgt gtccaatatt
    26281 ctagccaagt tagaagttga tgaccgaacc caagcggttg tctatgcttt tcgacatcat
    26341 ttagtccccc aagacgataa ttaatgctat aatatagggt atagcaaatt ggaggaaagt
    26401 atggacgcaa aactaaaata caaagctaaa aaaatcaaaa tggtgttctt tgatattgat
    26461 gataccttga gggtcaaaga tacgggttat atgcctgagt cgattcaaag agtatttaag
    26521 gctttgaagg caaaaggcat cttggttggt attgcttcag gtcgcgctcg gtacggtgtt
    26581 ccacaagaag ttcaagattt acatgctgac tattgtgtca aattaaatgg agcttatgtc
    26641 aaagacgatg ctaaaactat tattttccaa gctccgattc cagcagatgt tgttgtggcc
    26701 tataaaaaat gggcggatga tatgggcatt ttttatggaa tggcgggtcg tcatgaagcg
    26761 gtgttgtccg ctcgaaatga catgattagc aatgctattg ataatgtcta cgctcaacta
    26821 gaggtctgtc cggattataa tgagtatcat gatgtttacc agatgtggac tttcgaggac
    26881 aagggagatg gcttgcagtt gcctgctgaa ttagcagagc accttcgttt ggtcagatgg
    26941 cacgataact catcagatgt tgtcttgaaa ggtacgtcaa aagcgctagg tgtttcaaaa
    27001 gtagttgacc atttaggatt aaaacctgaa aacattttgg tttttggaga tgaactcaat
    27061 gacctagagt tatttgacta tgctggtatc agtattgcaa tgggagtatc acatccatta
    27121 ctgcaagaaa aagcagattt tatcacaaaa aaagttaaag aagatggcat tttatatgcc
    27181 ttggaggaat taggattgat tgacaaagaa ttacagtttc cacaattgga tttgccaaac
    27241 cataaaggac caaaagcaac cattaaaaca aaccatggcg atatgaccct ggtcttgttc
    27301 ccagatcatg ctcctaaaac agtagccaat ttcttaggtt tggctaaaga agggtactat
    27361 gatgggatta tcttccaccg tattattcct gaatttatga ttcaaggggg cgatccaaca
    27421 ggaacaggaa tgggtggaca atccatctat ggtgaaagtt ttgaagatga attttcggat
    27481 gaactctata atctccgtgg ggccttgtcg atggctaatg cgggaccaaa tactaacggc
    27541 agtcagttct ttattgttca aaatagtaag attccttatg ccaaaaaaga actagaacgt
    27601 ggtggttggc cggctccaat tgcagcttct tacgctgcaa ttggtggaac tcctcacctt
    27661 gatcgtcgtc atacggtctt tggtcagctt gtggacgaaa cttcattcca agttttggat
    27721 ttgattgctg gcgttgaaac tggcgcacag gataaaccta aagaagacgt tatcattgaa
    27781 acaatcgagg tatttgacta atgaaaattg gcgacaaact gcatggcacc atcactggga
    27841 ttaaaccata cggagccttt gttgcccttg aaaatgggac aacaggtctc attcatattt
    27901 cagaaataaa aacgggattt attgacgata ttgatcaatt attggctata ggcaaccagg
    27961 ttttagtaca agtaattgat attgatgaat atagtaaaaa gccaagttta tctatgcgga
    28021 ccttagcaga agaaaaacag cattttttcc atcgtcatcg ttattccaac agtcggcaca
    28081 agattggctt taggccactt gaagagcagt tacctcagtg gattgaagaa agcctgcagt
    28141 ttttaaaaaa gaacactaaa aagagtctgg aaccttagta gttccaaacc cttttttatt
    28201 tcgtcttaat tactggcaca tcatagagtt cagtcgataa atagcgttcg ccattatctg
    28261 gtaaaatagt taacacatgt ttgccttttc ctaattgttt agcgacttca atagcggcgt
    28321 aaagagcagc tccagaagaa atcccaacca ggaagccttc cttagctcct gttagtcgag
    28381 cagtttctaa agcatctttc gatttaacac ggataatttg gtcataggct ttggtatcta
    28441 acgtgtttgg gataaatcca gctgatatac cttgaatttt atgtggtcca ggctcttgac
    28501 cagataagac agcagattct tcagcttcaa cagcatagat aacagtttca gggctagctt
    28561 ttttcaagac atgtgaaaca ccagaaagtg ttcctccagt accaacacca gatacgaatg
    28621 catctaaaga aatctcctta aaagcttcca aaatttcttg agctgttgtt ttttcatgga
    28681 tgcttggatt ggcagggtta ttaaattgca taggcatcca agcacctagt tctattgcta
    28741 aagtttcagc ttttgcaata gcccctttca taccttctgc tccaggtgtt aagacaagct
    28801 ctgcaccata agcctgaatg atttgtcgtc tttccaagct catagtttcg ggcataacaa
    28861 taatgactcg atacccttta gcagcaccta cccatgcaag accaatacct gtattaccgc
    28921 ttgttggttc gataataacg tcaccaggac ttatcagacc ttcagcttca gcagcttcaa
    28981 tcatcgataa agcaatacga tctttaacag aagatcctgg gttaaaagct tctaatttta
    29041 cataaacgtc agcagcttcg tttggaatta aacagttaag tttgataata ggtgtttgac
    29101 ctactaattc tgttatagtt ttgtaaattt tagtcatagt ttagcctctt ttcttgatac
    29161 aaaataaata cgctgtgtat ttaagtataa ggagagatat tattaaagta aaatacagat
    29221 ttactatcac tctgataata aagccatatc acttttgttg atgattaatc acaggggaat
    29281 tcaataactt tggatccgat tttctcacag cttatctgac catgataata atttgttaaa
    29341 gtatcaatga gattttccac acgttcagta tcacaataaa gtgttgtctt tatcgtgtct
    29401 gaaaacttag tttctgtttc ggttaaagcc aattgatcta gcaagttaga gtatagttgg
    29461 tattggggat aagaaagtgt gatttctagt cctatctgct gtttaacttc aatgatgcca
    29521 aatcgtttaa tagctgttgc ggtcatgtta gagtatgctc tgattagccc acctgttcct
    29581 aatttaatac caccaaagta acgcgttacc acaactacta catttgttaa acactgcttt
    29641 tctaacacag aaaggatagg tattccagca gttcctgaag gttcaccatc atcacttgag
    29701 cgcttgatct gactgttgtt accgataatc atcgcaaagc aggaatgatt agctttgtaa
    29761 tgttcttttt tgatagcatt aacaaaattc ttaccatctt cttcagtgct tactcgtttg
    29821 atatgacaaa tgaaacgaga ttttttaata ctttcttcga aaaaaccact ggctttgatt
    29881 gtcttaaaat gttccataaa aaaattgtac aagaaaagat aaaaattagc aaaaaaaagc
    29941 gaataataaa gtatggagtc tattgaaaat agttatggac gattgttgct agaaagccag
    30001 ttaccagatt cagcaaagca gcttgctcaa cctttaaaaa gtgtagtaat atcgagagga
    30061 aaaatgattt gtcaacgttg tcactatcag cttgatgaag aagcacgatt gccaaatgga
    30121 gcctattatt gccgattttg tcttgtcttt ggacgaaatc aatcagataa attgctctat
    30181 gctatcccac ctatgcattt tccaaaaggt aattatctgg tttggagcgg tcaactaact
    30241 gcttatcaag agatgatttc ccaacaatta ttaatcaata tgcaaaatca aaaaacaaca
    30301 ttagttcatg ctgtaactgg tgcaggaaaa acagaaatga tttatgctgc gattgaggcg
    30361 gtaattaaca caggtggctg ggtttgcatt gccagcccaa gagtagatgt ttgtgtagag
    30421 gtggctactc gtttatccca agccttctcc tgcagcatct gtttaatgca cgcagagtct
    30481 cttccttatc aaagagctcc tattatagta gccacaacgc atcagctgct aaaatttcac
    30541 aaggcttttg atctcttaat tattgatgaa gtagacgcat tcccttttgt aaataatatt
    30601 caacttcatt atgctgcaag tcaggcttta aaagaaggtg gcgcaaagat tttattaact
    30661 gcaacttcaa ctagaacttt agaaagaaag gtaaacaagg gagaagtagt caagctgaca
    30721 ttggcgagac gctttaataa taggccatta gttattccga aatttataag aagttttaat
    30781 ctatttaaaa tgatacatcg gcaaaaactt cctcctaaaa ttttaaaata cttaaaaaaa
    30841 caaagaaaaa caggctatcc ccttttaatt tttctaccca ctattataat ggcagaatct
    30901 gttacagcaa tcttaaaaga gttattgccc gccgaacaaa tagcttgtgt ttctagtcaa
    30961 tcacaaaaca ggaaagaaga tatcacagct tttcgtcaag ggaaaaaaac gattttaatt
    31021 acaacaacaa ttttagaaag gggtgtgaca tttccccaaa ttgatgtttt tgttcttggg
    31081 tctcatcatc gtgtctatag ttcccaaagt cttgttcaaa ttgctggacg agttggaaga
    31141 tctatagaca gaccagacgg aacgttatat ttctttcatg aaggtatcag taaagcaatg
    31201 ctactagcaa gaaaagagat taaagaaatg aattataaag ggtattcgca tgatctgtct
    31261 actatgtcaa caaattagtc aaacaccaat aagtattaca gaaatcatct ttttaaggcg
    31321 tatctcttca ccgatttgtc aacaatgtca aaaaagcttt caaaagatag gaaaaagtgt
    31381 ttgtgcgaca tgttgtgcaa actcagatat aatagcttgt cgagattgtc taaaatggga
    31441 aaacaaagga tacaatgtaa atcatagaag cttatattgt tataatgctg ctatgaaagc
    31501 atacttcagt caatataagt ttcaaggaga ctatttatta agaaaagttt ttgcagtaga
    31561 acttgccgat gttattacca agtactataa aggctatatc ccagtcccgg ttcctgtaag
    31621 tcccggttgt tttcgagaaa gacaatttaa tcaagtgagc gctattcttg aggcagctaa
    31681 tgttagctac ctttctcttt ttgaaaagct agataatact caccaatctt ccagaacaaa
    31741 aaaagagaga ttattagtag aaaaatctta tcgactacta aaagtatcaa acattcctga
    31801 taaaatcctt atagtagatg atatttatac tactggtagt acaattatcg ctcttagaaa
    31861 acaattggct aaagtagcaa atagtgacat taaaagtttg tcaattgcac gttaaaactt
    31921 gcaaattttc atgaaagcag tataatagta ttaaagataa agtaagcgct tcaagcgcga
    31981 gaaagaggta tcctatgatt aagttcagta ttcgtggaga aaatatcgaa gtgacagaag
    32041 ctattcgcga ttatgtagaa tcaaagctta ctaaaatcga aaaatatttt gctaaagacc
    32101 aagaaataga tgcacgtgtt aatttaaaag tttatcggga gagatcttca aaagttgagg
    32161 taacgatccc attagattca gtaacccttc gtgctgaaga tgtttcacag gatatgtacg
    32221 gctctattga tttagttgtt gataagatcg aacgccaaat tagaaaaaat aaaaccaaaa
    32281 ttgctaaaaa acatcgcgaa aaggttccaa ctggtcaagt ctttacaact gagtttgaag
    32341 ctgaagaagt cgatgagata ccagaagtac aagttgtacg tactaaaaat gtgacattaa
    32401 aaccaatgga tgttgaagaa gcgcgtttac agatggaact attgggacat gatttcttta
    32461 tctacactga ttctgaagat ggtgcaacaa acattttata ccgtcgcgaa gatggaaacc
    32521 taggattaat agaagctaaa tagttcacaa ctaaaaccta cagggatgat tatacgtatt
    32581 ttaaaagtgg gaaaaagcta ttcatgtttc gaggtgacat gatgaaaatg agattaagtg
    32641 atagatacga cagtcattat ataacaaaag tatctattac agttcctctt taagtaaatc
    32701 ttacgtaaaa ttagtttgaa aaaaagatta gtcccaattc tgggctagaa attctcaaaa
    32761 aatatgaaaa agcgcgtttt cagaatataa agcgcgtttt tttctttttc catttaacaa
    32821 taaaaaactc atgacgaaca tggtat
//
LOCUS       DATXQK010000016        32665 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.016, whole genome shotgun sequence.
ACCESSION   DATXQK010000016 DATXQK010000000
VERSION     DATXQK010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32665
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.016"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            78..869
                     /locus_tag="VC394_000832"
     CDS             78..869
                     /locus_tag="VC394_000832"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEP5994311.1"
                     /translation="MQFKHIIFDVVDDLATLTLNRPEVSNGFNIPICQEILVALAEVK
                     RDTSVRFLLIKAVGKVFSVGGDLVEMQEAVAKDNVQSLVKIAELVQEISFAIKHLPKP
                     VILCADGAVAGAAFNIALAVDFCIASTQTKFIQAFVNVGLAPDAGGLFLLTRAVGLNR
                     ATHLVMTGEGITADKGLDYGFVYRTAESDKLDKVCLQLLKRLRRGSSNSYAGMKSLVW
                     QSFFTGWEDYAKAELAIQEELAFKEDFKEGVIAFGERRRPNFQGK"
     gene            946..1380
                     /locus_tag="VC394_000833"
     CDS             946..1380
                     /locus_tag="VC394_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEP5994312.1"
                     /translation="MEYDKINQYLVDIFNRILVIEEMSLKTSQFNDVSLKEMHTIEII
                     GKYDQVTPSDIARELMVTLGTVTTSLNKLEAKGYIARTRSRSDRRVVYLSLTKRGRLL
                     DRLHAKFHKNMVGHVIADMSDEEMQALVRGLGNLHQFLEDLV"
     gene            1381..2355
                     /locus_tag="VC394_000834"
     CDS             1381..2355
                     /locus_tag="VC394_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEP5994313.1"
                     /translation="MIFSKISQVAHYVPQQLVTNNDLASIMDTSHEWIFSRTGIAERH
                     ISRDEMTSDLAIQVADQLLTQSGLKADAIDFIIVATISPDATMPSTAAKVQAAIAATS
                     AFAFDMTAACSGFVFALAMADKLIASGAYQNGMVIGAETLSKLVNWQDRATAVLFGDG
                     AGGVLLEASKDKHVLAETLHTDGARCQSLISGETSLSSPYSIGKKAIATIQMDGRAIF
                     DFAIRDVSKSILTLMAQSDITKDDIDYCLLHQANRRILDKIARKIDVPREKFLENMMR
                     YGNTSAASIPILLSEAVQKGQIRLDGTQKILLSGFGGGLTWGSLIVKI"
     gene            2416..2640
                     /locus_tag="VC394_000835"
     CDS             2416..2640
                     /locus_tag="VC394_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEP5994314.1"
                     /translation="MAVFEKVQEIIVEELGKETEEVTLETTFDDLDADSLDVFQVISE
                     IEDAFDIQIETEEGLNTVGDLVAYVEEKSK"
     gene            2842..3813
                     /gene="fabK"
                     /locus_tag="VC394_000836"
     CDS             2842..3813
                     /gene="fabK"
                     /locus_tag="VC394_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEP5994315.1"
                     /translation="MKTRITELLNIDYPIFQGGMAWVADGDLAGAVSNAGGLGIIGGG
                     NAPKEVVKANIDRVKAITDRPFGVNIMLLSPFADDIVDLVIEEGVKVVTTGAGNPGKY
                     MERLHQAGIIVVPVVPSVALAKRMEKLGVDAVIAEGMEAGGHIGKLTTMSLVRQVVEA
                     VSIPVIAAGGIADGHGAAAAFMLGAEAVQIGTRFVVAKESNAHQNFKDKILAAKDIDT
                     VISAQVVGHPVRSIKNKLTSAYAKAEKAFLIGQKTATDIEEMGAGSLRHAVIEGDVVN
                     GSVMAGQIAGLVRKEESCETILKDIYYGAARVIQNEAKRWQSVSIEK"
     gene            3838..4776
                     /gene="fabD"
                     /locus_tag="VC394_000837"
     CDS             3838..4776
                     /gene="fabD"
                     /locus_tag="VC394_000837"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEP5994316.1"
                     /translation="MTKTAFLFAGQGAQKLGMARDFYDNFAIVRKTFDQASQVLGYDL
                     RRLIDSDELKLNQTSYTQPAILTSSIAIYRVLGLHHVKPDMVAGLSLGEYSALVASGA
                     LSFEDTLSLVAKRGRLMEEAAPQGSGKMVAVMNTDVQVIEEVCQIAAKHGVVAPANYN
                     TPSQIVIGGQTDAVNVAVELLKERGVKRLIPLNVSGPFHTALLEPASRLLAKELERYN
                     FSDFKIPLVGNTEANIMEKDRIPELLARQVMEPVRFYDSVATLVESGITQFIEVGPGK
                     VLTGFVKKIDKNLLCTSVENMVSLRLFLDREDIHGN"
     gene            4766..5500
                     /gene="fabG"
                     /locus_tag="VC394_000838"
     CDS             4766..5500
                     /gene="fabG"
                     /locus_tag="VC394_000838"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEP5994317.1"
                     /translation="MEIKGKNIFITGSTRGIGLAMAHQFASLGANIVLNGRSAISEEL
                     VASFTDYGVTVVTISGDVSEASEAKRMVNEAIESLGSIDVLVNNAGITNDKLMLKMTE
                     EDFERVLKINLTGAFNMTQSVLKPMIKARQGAIINVSSVVGLTGNIGQANYAASKAGM
                     IGFTKSVAREVAARNICVNAIAPGFIESDMTGVLPEKMQEQILSQIPMKRIGKAQEVA
                     HLASFLVEQDYITGQVIAIDGGMTMQ"
     gene            5515..6747
                     /gene="fabF"
                     /locus_tag="VC394_000839"
     CDS             5515..6747
                     /gene="fabF"
                     /locus_tag="VC394_000839"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEP5994318.1"
                     /translation="MTFKRVVVTGYGLTSPIGHDPETFWNNLKAGQIGIGPITKFDTT
                     DYAVKNAAEIQDFPFDKYFVKKDLNRFDRYSLYALYAAKEAINHADLNIEMVDSDRFG
                     VIVASGIGGIAEIEEQVIRLHEKGPKRVKPMTLPKALPNMAAGNVAMSLKAQGVCKSI
                     NTACASSNDAIGDAFRAIKFGTQDVMIVGGSEAAITKFAIAGFQSLTALSTTEDPSRS
                     SIPFDKDRNGFIMGEGSGMLVLESLEHAQERGATILAEIVGYGNTCDAYHMTSPNPEG
                     LGARKAIHLALQEAGIEASAINYVNAHGTSTPANEKGESQAIVAVLGKDVPVSSTKSF
                     TGHLLGAAGAIEAIATIEAMRHNYVPMTAGTQALSEDIEANVIFGEGKETAINYAISN
                     TFGFGGHNAVLAFKCWEE"
     gene            6749..7249
                     /locus_tag="VC394_000840"
     CDS             6749..7249
                     /locus_tag="VC394_000840"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEP5994319.1"
                     /translation="MNIQEIKDLMAQFDTSSLREFLFKTNEGELIFSKNEQHLNASTS
                     NQEHAVPVPQVQLVPNSTASEASSPASVKDVPVEEQPQAESFVAEGDIVESPLVGVAY
                     LAASPDKPPFVAVGDTVKKGQTLVIIEAMKVMNEVPAPCDGVITEILVSNEDVIEFGQ
                     GLVRIK"
     gene            7246..7668
                     /gene="fabZ"
                     /locus_tag="VC394_000841"
     CDS             7246..7668
                     /gene="fabZ"
                     /locus_tag="VC394_000841"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002922202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEP5994320.1"
                     /translation="MMDIREIQAALPHRYPMLLVDRVLEVSDDHIVAIKNVTINEPFF
                     NGHFPHYPVMPGVLIMEALAQTAGVLELSKEENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFIKRRGTIAVVEARAEVDGKLAASGTLTFACGQ"
     gene            7700..9064
                     /locus_tag="VC394_000842"
     CDS             7700..9064
                     /locus_tag="VC394_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEP5994321.1"
                     /translation="MFKKILIANRGEIAVRIIRAARELGISTVAVYSEADKEALHTIL
                     ADEAICIGPARSKESYLNMNSVLSAAIVTGAQAIHPGFGFLSENSKFATMCEEMNIKF
                     IGPSASVMDKMGDKINARSEMIKAGVPVIPGSDGEVYNAQEALAIANKIGYPVMLKAS
                     AGGGGKGIRKVETEADLEAAFNAASQEALGAFGNGAMYLEKVIYPARHIEVQILGDAH
                     GNIIHLGERDCSLQRNNQKVLEESPSIAIGNTLRHEMGQAAVRAAEAVAYENAGTIEF
                     LLDEDSEKFYFMEMNTRIQVEHPVTEFVTGVDIVKEQIKIAAGQPLAISQEDITITGH
                     AIECRINAENTAFNFAPSPGKITDLYMPSGGVGLRVDSAVYNGYAIPPYYDSMIAKII
                     VHGSNRFDALMKMQRALVELEIEGIITNTDFQLDLISDKRVIAGDYDTSFLMETFLPH
                     YNKD"
     gene            9073..9939
                     /locus_tag="VC394_000843"
     CDS             9073..9939
                     /locus_tag="VC394_000843"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEP5994322.1"
                     /translation="MALFRKKDKYIRITPNNSLKGSVSHNVPEVPDELFAKCPACKHM
                     IYKKDLGLAKICPTCSYNFRISAQERLTLTVDEGSFQELFTSIETKDPLRFPGYQEKL
                     QKAKETTGLHEAVLTGKAMVKGQQIALAIMDSHFIMASMGTVVGEKITRLFELAIEEN
                     LPVVIFTASGGARMQEGIMSLMQMAKVSAAVKRHSNAGLFYLTILTDPTTGGVTASFA
                     MEGDIILAEPQSLVGFAGRRVIETTVRENLPDDFQKAEFLQDHGFVDAIVKRTELRDK
                     IAHLVAFHGGGQ"
     gene            9936..10706
                     /locus_tag="VC394_000844"
     CDS             9936..10706
                     /locus_tag="VC394_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEP5994323.1"
                     /translation="MTDVSRILKEARDQGRLTTLDYANLIFDDFMELHGDRHFSDDGA
                     IVGGLAYLAGQPVTVIGIQKGKNLQDNLARNFGQPNPEGYRKALRLMKQAEKFGRPVV
                     TFINTAGAYPGVGAEERGQGEAIAKNLMEMSDLKVPIIAIIIGEGGSGGALALAVADQ
                     VWMLENTMYAVLSPEGFASILWKDGSRATEAAELMKITAGELYQMGIVDRIIPEHGYF
                     SSEIVDIIKANLIEQITSLQAKPLDQLLDERYQRFRKY"
     gene            complement(10939..12216)
                     /gene="serS"
                     /locus_tag="VC394_000845"
     CDS             complement(10939..12216)
                     /gene="serS"
                     /locus_tag="VC394_000845"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEP5994324.1"
                     /translation="MLDLKRIRTDFDTVAAKLKNRGVSEDTLTHLKELDEKRRALLVQ
                     SEELKAERNIASAAIAQAKRQKEDATQQIADMQKVSADIKTIDNQLVAIDQQVTDIIT
                     VLPNTPHDSVPVGADEEDNVEIRRWGTPRDFDFEVKAHWDLGEDLDILDWERGAKVTG
                     ARFLFYKNLGARLERALYNFMLDEHIKEGYQEIITPYMVNHDSMFGTGQYPKFKEDTF
                     ELADTNFVLIPTAEVPLTNYYRGEILDGKELPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYQELEKMTANAENILQKLGLPYRVISLCTGDMGFSAAKTY
                     DLEVWIPAQNTYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEVLRSYMGGETVISPK"
     gene            complement(12608..12967)
                     /locus_tag="VC394_000846"
     CDS             complement(12608..12967)
                     /locus_tag="VC394_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEP5994325.1"
                     /translation="MAQSLNTSVEYKTKAVSYLGIGGKVGHILLGDKALEFYNDKNVN
                     DYIQIPWTAINHIGANVSRKKVSRHFEIFTDQGKFLFASGDSGKILKITRQHIGNEKV
                     ITLPTLMQTFINKFFRH"
     gene            complement(13081..13992)
                     /locus_tag="VC394_000847"
     CDS             complement(13081..13992)
                     /locus_tag="VC394_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEP5994326.1"
                     /translation="MTEQIKLTKSDRQRVWWRSQFLQGSWNYERMQNMGWAYALIPAL
                     KKLYTSPEDRAAALERHMEFFNTHPYVAAPIIGVTLALEEERANGTPIDDKAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGALGASLASTGNIVGPLLFFFGWNLIRMAFLWYTQE
                     FGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVSINFTIDLPGKQLSDGA
                     YVVFPDGAVKGAELKTILANAIGGMSLDKVQAQTLQGQLDSLIPGLAGLLLTFLCMWL
                     LKKKVSPIAIIIGLFAFGILAHLAGIM"
     gene            complement(14009..14818)
                     /locus_tag="VC394_000848"
     CDS             complement(14009..14818)
                     /locus_tag="VC394_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEP5994327.1"
                     /translation="MSDISIISAILVVIIAFFAGLEGILDQFQMHQPLVACTLIGLVT
                     GHLEAGVILGGTLQMLALGWANIGAAVAPDAALASVAAAIIMVKSGDFTQKGITFAYS
                     TAIPLAVAGLFLTMIVRTLSTALVHAGDKAATEGNFAGIERFHFIALLLQGLRIAVPA
                     ALLVAVPTSAVQSVLNAMPNWLNEGMQIGGAMVVAVGYAMVINMMATREVWPFFALGF
                     ALAAISQLTLIAMGVIGVAIAFIYLNLSKKGGNGGNAAGSADPIGDILEDY"
     gene            complement(14907..15899)
                     /gene="manX"
                     /locus_tag="VC394_000849"
     CDS             complement(14907..15899)
                     /gene="manX"
                     /locus_tag="VC394_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEP5994328.1"
                     /translation="MGIGIIIASHGKFAEGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYGHFNNAIQQFDADDEILVLADLWSGSPFNQASRVAGENPDRKMAIITGLNLPMLIQ
                     AYTERLMDAGAGVEQVAANIIKESKDGIKALPEDLNPVEETAATEKVVNALQGAIPAG
                     TVIGDGKLKINLARVDTRLLHGQVATAWTPASKADRIIVASDEVAQDDLRKQLIKQAA
                     PGGVKANVVPISKLIEASKDPRFGNTHALILFQTPQDALRAVEGGVEINELNVGSMAH
                     STGKTMVNNVLSMDKEDVATFEKLRDLGVTFDVRKVPNDSKKNLFELIQKANIK"
     gene            complement(16251..17063)
                     /locus_tag="VC394_000850"
     CDS             complement(16251..17063)
                     /locus_tag="VC394_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEP5994329.1"
                     /translation="MTKKLIAIDLDGTLLHHDNTISTYTQKIIKAVQDKGHHVIISTG
                     RPYRMALDYYLQLNLKTPIITFNGALTHMPEQKWAYEHNVTLDKGYLLRLLKYQDDFQ
                     MDFIASEYRKNVYITMTNPESIDPQLFGVDEITQDMALEITKITRNPNALLMQTHHED
                     KYALAKNMRAFFKDEIEIDSWGGPLNILEISSKNVNKAYALNYLLGIYNMDKKDLIAF
                     GDEHNDTEMLAFAGTGYAMKNASPVLLPYADQQLNFSNEEDGVAKKLEELFL"
     gene            17327..18787
                     /locus_tag="VC394_000851"
     CDS             17327..18787
                     /locus_tag="VC394_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEP5994330.1"
                     /translation="MEKFFKLSENGTTVSTEIMAGLTTFFAMSYILFVNPSILGAAGM
                     PSNAVFLATIIAAAISTLIMGLFANVPYALAPGMGLNAFFTYTVVFALGFSWQEALAM
                     VFICGLFNIFITVTKFRKSIIKAIPVSLQHAIGGGIGVFVAYLGFKNANIITFSISAE
                     NIVMVNGVEPAKASAKTFADGLLFVDANGGVVPTISSFTDSSVLLAIFGLLLTTALVI
                     RNFRGAILIGIVATTLVGIPLGIVDVSNLNFGISHIGEAWTELGTTFLAAFDGLSSLF
                     SDSSRLPLVFMTIFAFSLSDTFDTIGTFIGTGRRTGIFSQDDENALENSIGFSSKMDR
                     ALFADAIGTSIGALVGTSNTTTYVESAAGIAEGGRTGLTAVSTAVCFLLSILLLPLVG
                     IVPAAATAPALIVVGVMMVSSFLDVNWSKFADALPAFFAAFFMALCYSISYGIAAAFI
                     FYCLVKVVEGKTKDIHPIIWGATFLFIVNFIILTIL"
     gene            18941..19402
                     /gene="tsaE"
                     /locus_tag="VC394_000852"
     CDS             18941..19402
                     /gene="tsaE"
                     /locus_tag="VC394_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEP5994331.1"
                     /translation="MFYSENEYTLKAYGETLGTYLSIGDVIVLSGDLGAGKTTLAKGI
                     AKGMGISQMIKSPTYTIVREYEGRLPLYHLDIYRVGDDPDSIDLDDFLFGNGVTVIEW
                     GELLGEGLLQDYLQITITKRDKGRQLDLLAHGERSRQLLEIMSHDSAKSRY"
     gene            19377..19901
                     /locus_tag="VC394_000853"
     CDS             19377..19901
                     /locus_tag="VC394_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP5994332.1"
                     /translation="MIQQSLVIEEGVPQDAKAVLSLVAAAAKETDFITGVETILEATP
                     HELSDFLSRSQTSFIDFCLLARLDEKVVGLLNLSGEVLSQGQVEADVFMLVAKTYRGY
                     GIGQLLLEIALDWAEENPYIESLKLDVQVRNTKAIYLYKKYGFRIESMRKNDIKSKNG
                     DDLDVYHMRKSVSQ"
     gene            19910..21193
                     /locus_tag="VC394_000854"
     CDS             19910..21193
                     /locus_tag="VC394_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEP5994333.1"
                     /translation="MKIGKKIVLMFTAIVLTTVLALGVYLTSAYTFSTGELSKTFKDF
                     STSSKKSDAIKQTRAFSILLMGVDTGSSERASKWEGNSDSMILVTVNPKTKKTTMTSL
                     ERDTLTTLSGPKNNEMNGVEAKLNAAYAAGGAQMAIMTVQDLLNITIDNYVQINMQGL
                     IDLVNAVGGITVTNEFDFPISIAENEPEYQATVAPGTHKINGEQALVYARMRYDDPEG
                     DYGRQKRQREVIQKVLKKILALDSISSYRKILSAVSSNMQTNIEISSRTIPSLLGYRD
                     ALRTIKTYQLKGEDATLSDGGSYQIVTSNHLLEIQNRIRTELGLHKVSQLKTNATVYE
                     NLYGSTKSQTVNNNYDSSGQAPSYSDSHSSYANYSSGVDTGSSYQSASTDQDSTASSH
                     TPTTPSSSSDALAADESSSSGSGSLVPPANINPQT"
     gene            complement(21304..21660)
                     /locus_tag="VC394_000855"
     CDS             complement(21304..21660)
                     /locus_tag="VC394_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HEP5994334.1"
                     /translation="MKVKTVIAVGLLSFTAYKAYQKRCTIKELLSISRQAKDAAQLDL
                     DNIKANLDLIHSQGKVIQNISQDLAHKWRYFNQETQAHLTEIQNRMAKYQEDSELISS
                     ESPTCQTVILTNNHSR"
     gene            complement(21657..22076)
                     /locus_tag="VC394_000856"
     CDS             complement(21657..22076)
                     /locus_tag="VC394_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEP5994335.1"
                     /translation="MENCIFCSIIQGDIPSSKVYEDEQVLAFLDISQTTKGHTLVIPK
                     QHVRNLLEMTAETASHLFARIPKIARAIQLATGATAMNIINNNEALAGQTVFHAHVHL
                     VPRYNEKDGISIQYTTHEPNFPVLEKLARQINQEVSS"
     gene            22148..22873
                     /locus_tag="VC394_000857"
     CDS             22148..22873
                     /locus_tag="VC394_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5994336.1"
                     /translation="MLNIKNLTGGYHNIPVLNDVSFSVDNGELVGLIGLNGAGKSTTI
                     NEIIGFLKPYQGSISIDGLTLAENAVAYRQKIGFIPETPSLYEELTLSEHINTVAMAY
                     DIDLEVAQKRAQPFLEMFRLTDKLEWFPVNFSKGMKQKVMIICAFVIDPSLFILDEPF
                     LGLDPLAISDLIQTLEVEKAKGKSILMSTHVLDSAERMCDRFVILHHGQVRAQGTLAD
                     LQEAFGDRSASLNDIYLALTKED"
     gene            22876..23910
                     /locus_tag="VC394_000858"
     CDS             22876..23910
                     /locus_tag="VC394_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP5994337.1"
                     /translation="MKALFLKRRQDFQKQQNKYLRYVLNDHFVLVLMFLLGYAMVQYG
                     QLLNHFPTNHLPIQVCLGILIPLLLSMGSIATYLEEADQHFLLPKEEEVISYIKQAER
                     LSFLLWGTLQTAILLFLYPIFRRLGLSLFIFIILVLILLALKRVVLSRKTRYFLRGNR
                     LDWAKAVAFESNRKQSILKFYSLFTTVKGISTKVKERTYLNPLLKLVKQTPSNLWLSL
                     YARAFLRSSDYLGLFLRLMLLSSLSVFFIHNLYLSVSLALIFNYLVVFQLLSLYYHYD
                     YHYMTSLYPENSRSKKKNVLSFLRGLSFLMLIVNMLCCSSAPKALILIVGMVFIACIY
                     LPYKLKKIID"
     gene            23974..24765
                     /locus_tag="VC394_000859"
     CDS             23974..24765
                     /locus_tag="VC394_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEP5994338.1"
                     /translation="MTTTEQELTLTPLRGKSGKAYKGTYPNGECVFIKLNTTPILPAL
                     AKEQIAPQLLWAKRMGNGDMMSAQEWLNGRTLTKEDMNSKQIIHILLRLHKSKKLVNQ
                     LLQLNYKIENPYDLLVDFEQNAPLQIQQNSYLQAIVKELKRSLPEFKSEVATIVHGDI
                     KHSNWVITTSGMIFLVDWDSVRLTDRMYDVAYLLSHYIPRSRWSEWLSYYGYKNNDKV
                     MQKIIWYGQFSHLTQILKCFDKRDMEHVNQEIYALRKFREIFRKK"
     gene            24765..25400
                     /gene="trmB"
                     /locus_tag="VC394_000860"
     CDS             24765..25400
                     /gene="trmB"
                     /locus_tag="VC394_000860"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEP5994339.1"
                     /translation="MRVRKRKGAEEHLANNPHYVILNPEDAKGRWHDVFGNDRPIHIE
                     VGSGKGGFITGMALKNPDINYIGIDIQLSVLSYALDKVLASEVPNVKLLRVDGSSLTN
                     YFEDGEVDMMYLNFSDPWPKTKHEKRRLTYKDFLDTYKRILPEHGEIHFKTDNRGLFE
                     YSLASFSQYGMTLRQIWLDLHASNYEGNVMTEYEEKFSNKGQVIYRVEANF"
     gene            25465..25551
                     /locus_tag="VC394_000861"
     tRNA            25465..25551
                     /locus_tag="VC394_000861"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25499..25501,aa:Ser,seq:gga)
     gene            25659..26195
                     /gene="rimP"
                     /locus_tag="VC394_000862"
     CDS             25659..26195
                     /gene="rimP"
                     /locus_tag="VC394_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEP5994340.1"
                     /translation="MDSQGPIILEKSIKIEEVIKIANTSIIDIVTKTVTPEIKAPYEL
                     VDVEYDKMGSDYILSILVDKEGGITVEDTSDLTNIISPLLDTIDPDPFPNQYMLEVSS
                     PGLERPLKTADSLKAAVGSYINVSLYQAIDKVKVFQGDLLAFDGETLTIDYLDKTRHK
                     IVNIPYQAVAKVRMAVKL"
     gene            26370..27527
                     /gene="nusA"
                     /locus_tag="VC394_000863"
     CDS             26370..27527
                     /gene="nusA"
                     /locus_tag="VC394_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEP5994341.1"
                     /translation="MSKEMLEAFRILEEEKHIDKADIIDAVTESLKSAYKRRYGQSES
                     CVIEFNEKTADFQVFTVREVVEEVFDSRLEISLKDALAISSAYELGDKIRFEESVNEF
                     GRVAAQSAKQTIMEKMRRQMREVMFNEYKEHEGEIMTGTVERFDQRFIYVNLGSLEAQ
                     LSHQDQIPGETFKSHDRIDVYVYKVENNPKGVNVFVSRSHPEFIKRIMEQEIPEVFDG
                     TVEIMSVSREAGDRTKVAVRSHNPNVDAIGTIVGRGGSNIKKVISKFHPKRVDAKTGL
                     EIPVEENIDVIQWVDDPAEFIYNAIAPAEVDMVLFDDEDLKRATVVVPDSKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEYDRLEAEKEAATVVEEPVVDDVMTAEEV"
     gene            27543..27839
                     /locus_tag="VC394_000864"
     CDS             27543..27839
                     /locus_tag="VC394_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEP5994342.1"
                     /translation="MSKVKKIPLRKSLVSGEIIAKRDLLRIVKTKDGQVFIDPTGKQN
                     GRGAYIKLDNQEALMAKKKQVFNRSFSMDIPESFYDDLIAYVDHKIKRRELGLD"
     gene            27832..28134
                     /locus_tag="VC394_000865"
     CDS             27832..28134
                     /locus_tag="VC394_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEP5994343.1"
                     /translation="MTNLERLSSLIGLAQRAGKVISGEELVVKAIQHQQVILVFLAND
                     AGPNVTKKVTDKSNYYNVEVSTVLNALELSAALGKPRKVAAVADAGFSKKMRTLME"
     gene            28154..31015
                     /gene="infB"
                     /locus_tag="VC394_000866"
     CDS             28154..31015
                     /gene="infB"
                     /locus_tag="VC394_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEP5994344.1"
                     /translation="MSKKRLHEIAKEIGKSSKEVVEHAKYLGLDVKSHASSVEEADAK
                     KIISSFSKASKPDVTASQTVKPKEVAQPSVTVVKETGSEHVEKTQVSKPKSRNFKAER
                     EARAKEQAARKQANGSSHRSQERRGGYRQPNNHQTNEQGDKRITHRSQGDTNDKRIER
                     KASNVSPRHDNHQLVGDRNRSFAKENHKNGRFTNQKKQGRQEPQSKSPKIDFKARAAA
                     LKAEQNAEYSRQSETRFRAQQEAKRLAELARQEAKEAALKAQAEEMSHREAALKSIEE
                     AETKLKSSNISAKSTADNRRKKQARPEKNRELTHHSQEGQKKNKKSWNSQNQVRNQKN
                     SNWNKNKKTKKGKNVKNTNTAPKPVTERKFHELPKEFEYTEGMTVAEIAKRIKREPAE
                     IVKKLFMMGVMATQNQSLDGDTIELLMVDYGIEAKAKVEVDDADIERFFEDENYLNPE
                     NIVERAPVVTIMGHVDHGKTTLLDTLRNSRVATGEAGGITQHIGAYQIEEAGKKITFL
                     DTPGHAAFTSMRARGASVTDITILIVAADDGVMPQTIEAINHSKAAGVPIIVAINKID
                     KPGANPERVIAELAEYGIISTAWGGECEFVEISAKFNKNIDELLETVLLVAEVEELKA
                     DPTVRAIGTVIEARLDKGKGAIATLLVQQGTLHVQDPIVVGNTFGRVRAMVNDLGRRV
                     KSAEPSTPVSITGLNETPMAGDHFAVYADEKAARAAGEERSKRALLKQRQNTQRVSLD
                     NLFDTLKAGEIKTVNVIIKADVQGSVEALAASLVKIEVEGVRVNVVHSAVGAINESDV
                     TLAEASNAVIIGFNVRPTPQARQQADTDDVEIRLHSIIYKVIEEVEEAMKGKLDPVYQ
                     EKILGEAIIRETFKVSKVGTIGGFMVINGKVTRDSSVRVIRDSVVIFDGKLASLKHYK
                     DDVKEVGNAQEGGLMIENFNDLKVDDTIEAYIMEEIVRK"
     gene            31221..31571
                     /gene="rbfA"
                     /locus_tag="VC394_000867"
     CDS             31221..31571
                     /gene="rbfA"
                     /locus_tag="VC394_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEP5994345.1"
                     /translation="MANHRIDRVGMEIKREVNDILQKKVRDPRVQGVTITEVQMQGDL
                     SLAKVYYTIMSDLASDNQKAQTGLEKATGTIKRELGKQLTMYKIPDLVFEKDNSIAYG
                     NKIDQLLRELDKKD"
     gene            complement(31624..32625)
                     /locus_tag="VC394_000868"
     CDS             complement(31624..32625)
                     /locus_tag="VC394_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEP5994346.1"
                     /translation="MKKTLKSTLILLGLTLAIGASTQVDASSRSWKSWFIEKYFWLKG
                     DKGYYSEQDETKFDQYVNSAGEASNKGFDLKEEQVNGTLVTKTVDNMQVHTWNENPSN
                     KANQKVIFYLHGGSYLNNPTTYHIDMLKTLSTSLDAKIILPIYPKAPTYSYSDAMPKL
                     VDLYRDTLKETTAQNITIMGDSAGGGLALGLALALKNESLKQPKDIVLLSPWLDVTMS
                     NPDIESYESNDPILSAWGLRKVGEIWARRDPNNTKHPYVSPINGDVSGLAPITMFVGT
                     HEIFYPDVKKFDDILTAKGHQHELIVADKMNHVYPIYPIEEAKTAQYQIIDTIQKAY"
BASE COUNT        10077 a         5686 c         7046 g         9856 t
ORIGIN      
        1 taattgattt gattatcaaa tgattttaaa gagaaataat ttcttagtca aataaattat
       61 cagaaaagag gattgttatg cagtttaaac acatcatctt tgatgttgta gatgatcttg
      121 cgacattgac tttaaaccgt ccagaggttt caaacggttt taatattcca atttgtcaag
      181 agattctggt agcattagca gaagttaaac gcgatacatc tgtgcgtttt ttattaatta
      241 aagccgttgg aaaagtgttt tcagttggtg gtgacctagt tgaaatgcaa gaagctgttg
      301 caaaagataa tgtgcagtcg ctagtcaaga ttgctgaact ggtacaagaa atttcttttg
      361 caatcaagca tttgccaaaa ccagtcatcc tttgcgccga tggagctgta gctggagcgg
      421 cttttaatat agctttagct gttgactttt gtatcgctag tactcaaaca aaatttatcc
      481 aagcttttgt aaatgttgga ctagcaccgg atgccggtgg tttatttcta ttgactcgtg
      541 cagtaggact taatcgtgca actcaccttg tgatgacagg tgaaggaatt actgctgata
      601 aaggtctcga ttatgggttt gtctaccgta cggcagaatc tgataaacta gacaaagttt
      661 gtcttcaact tttaaagcga ttgagacgtg gctcttcaaa ttcttatgct ggaatgaaat
      721 cattggtctg gcaaagcttt ttcacaggat gggaagatta tgctaaggca gagcttgcta
      781 ttcaagagga gctagctttc aaggaagatt ttaaagaagg tgtcatagct tttggagaaa
      841 ggcgccgacc aaattttcaa ggtaagtaag acagctgttg cttgcaaaat actttgacct
      901 ttgatataat ttgattgtca aagtttattg ctaggaggtg tttacttgga atatgacaaa
      961 attaaccaat acttagttga tattttcaac cggattttgg ttattgaaga aatgagctta
     1021 aagacgagtc agtttaatga tgtctcttta aaagagatgc acactattga aattatcggc
     1081 aaatatgatc aggtgactcc aagtgatatc gccagagaat taatggtaac gttagggaca
     1141 gtaacgacta gcttaaataa attagaagca aaaggttata tcgcaagaac gcgatcacgc
     1201 tcagataggc gagttgttta cctgtcatta acaaaaaggg gtcgactttt ggaccgcctc
     1261 catgcaaagt tccataaaaa tatggttgga catgtcattg ctgatatgag tgatgaagag
     1321 atgcaggcat tggtgcgtgg actagggaat ctacatcaat ttttggagga tttggtttaa
     1381 atgatttttt ctaaaatcag tcaggtagcc cactatgttc cacagcagtt agtgactaat
     1441 aatgatttag ctagcataat ggacactagt catgagtgga tcttttctcg aacaggaata
     1501 gcagaacgcc atatttctag agatgaaatg actagtgacc tagctattca ggttgctgat
     1561 cagcttttaa ctcagtctgg gttaaaagct gatgctattg attttattat tgttgcaact
     1621 atcagccctg atgccactat gccatcaaca gctgcgaaag ttcaagcagc tattgcggct
     1681 acaagtgctt ttgcttttga tatgactgct gcttgtagtg gctttgtctt tgccttggct
     1741 atggcagata agcttattgc atcaggtgcc taccaaaacg gaatggtaat aggtgcagag
     1801 actttatcta agttagtaaa ctggcaggat cgagcgacag ccgttctttt tggagacgga
     1861 gctggtggcg tgctactgga agccagtaaa gataaacatg ttttagcgga aacattacac
     1921 acagatggtg cgagatgtca gagtttaatt tcaggagaaa ctagtctttc atctccgtac
     1981 tcaattggca aaaaagccat agcgactatt cagatggatg gacgtgccat atttgatttt
     2041 gcgattaggg atgtgtcaaa aagcatcctc acactaatgg cgcaatcaga tattacaaaa
     2101 gatgacatag actattgttt attgcatcaa gctaatcgtc gtatacttga taaaatagca
     2161 cgcaagattg atgttcctcg ggaaaaattc cttgaaaaca tgatgcgtta tggtaatact
     2221 agtgctgcta gtatcccaat tctcttgtca gaggctgttc aaaagggaca gattaggtta
     2281 gatggtaccc aaaaaatctt actttccggt tttggtggag gtttgacatg gggaagtcta
     2341 attgttaaaa tctagttatt catgtgccta gcacatttat aaaaacaaat ttatattttt
     2401 taaggagaaa taaacatggc agtatttgaa aaagtacaag aaattattgt tgaagaactt
     2461 ggtaaggaga cagaagaagt aacgctagaa actacatttg atgatttaga tgcagattca
     2521 cttgatgttt tccaagttat ttcagaaatt gaagatgctt ttgatattca aattgaaaca
     2581 gaagaaggat taaatactgt tggtgacctt gttgcttacg ttgaggaaaa gtcaaaataa
     2641 ggataaaagt gacaagtaac atcagaagtc tatgaaaagc ttactgctgt tatgttcatg
     2701 ttcatcacta aaaaaaacaa taagtattaa tcaaaccatt agggtgggat tagactcttt
     2761 ttaggatcta gctcgctctt tcttcttact ataatagttt gattatcaaa ttattctcct
     2821 tattttttat agaaagttat tatgaaaaca cgtattacag aattacttaa tattgattac
     2881 cccatttttc aaggaggaat ggcttgggtt gctgatggtg atttagcagg tgcagtttct
     2941 aatgctggtg gtttaggcat tataggtggt ggcaatgctc ccaaagaagt cgttaaagct
     3001 aatattgatc gtgtcaaagc tattactgat agaccttttg gggttaatat catgctttta
     3061 tctccttttg ctgatgatat cgttgatctg gtcattgaag aaggtgttaa agtagtaaca
     3121 acaggcgcag gaaatccagg aaagtatatg gaaagactgc accaggcggg tataatcgtt
     3181 gttcctgttg tcccaagcgt tgcgctagcc aaacgtatgg aaaagcttgg ggtagatgca
     3241 gtgattgctg agggtatgga agctggagga catattggca agttaacgac tatgtcttta
     3301 gtaagacaag ttgttgaagc ggtttcgatt cctgtcattg cggcaggtgg tatagctgat
     3361 ggtcatggtg cagcagcagc atttatgtta ggagcagagg ctgttcaaat tggaactcgc
     3421 tttgttgttg ctaaagaatc caatgcccac caaaatttta aagataaaat cttagcagca
     3481 aaagatattg atacggtgat ttctgcgcag gttgtgggcc accctgtccg ctctattaaa
     3541 aataaattga cctcagctta cgctaaagca gaaaaagcat ttttaattgg tcaaaaaaca
     3601 gctactgata ttgaagaaat gggagcagga tcgcttcgac atgctgttat tgaaggcgat
     3661 gtagtcaatg gatctgttat ggctggccaa attgcagggc ttgtgagaaa agaagaaagc
     3721 tgtgaaacga ttttaaaaga tatttattat ggtgcagctc gtgttattca aaatgaagct
     3781 aagcgctggc aatctgtttc aatagaaaag tagataaaca catgaaggat aatgataatg
     3841 acaaagacag cctttttatt tgccggtcaa ggtgcccaaa aattagggat ggcaagggat
     3901 ttttatgata actttgctat tgtaagaaaa acctttgatc aagctagtca agtattggga
     3961 tacgatttgc gccgtttgat tgatagtgac gagttaaaac ttaatcaaac tagctatacg
     4021 caaccagcta ttttgacatc atcaattgct atttaccgtg ttctgggctt acatcacgtt
     4081 aaaccggata tggtagctgg tctttcctta ggagagtact cagctttggt agcatcaggg
     4141 gcactctctt ttgaagatac cctatcctta gtagctaaga gaggccgctt gatggaggaa
     4201 gcagcaccac aaggatctgg aaagatggtt gccgttatga atacagacgt gcaagtcatc
     4261 gaagaagtct gtcaaatagc tgctaaacat ggagtggttg ctccagcaaa ctataatact
     4321 cctagtcaaa ttgtaattgg tggtcagaca gatgctgtga acgtggcagt tgaactttta
     4381 aaggaaaggg gagttaagcg tttaatccct ttaaacgtgt caggtccttt ccacactgct
     4441 ttgttagaac cagctagccg cttgttggct aaagagttgg aaagatacaa cttcagtgac
     4501 ttcaagattc ctttggttgg taataccgaa gctaatatta tggaaaaaga tcgtatccca
     4561 gaactattag cccgtcaagt catggagcct gttcgttttt atgacagtgt tgcgacttta
     4621 gtagaaagtg gcataacaca attcattgag gtaggaccag gtaaggtttt gacaggtttt
     4681 gtgaagaaaa ttgataaaaa tttactatgt actagtgttg aaaacatggt aagcttacgt
     4741 ttatttttag atagagagga tattcatgga aattaaagga aaaaatatat ttatcacagg
     4801 ctcaacgaga ggtatcggtt tagcaatggc tcatcaattc gccagtttag gagctaatat
     4861 tgtcttaaat ggtcgctctg ctatctctga ggagcttgtt gctagtttta ctgactatgg
     4921 tgttacagtt gttaccatct ctggggatgt ttcagaagca tcagaagcta aacgtatggt
     4981 caatgaagct atcgaaagtt taggttcgat tgatgtttta gttaacaatg ctggcattac
     5041 taatgataaa ttgatgctta aaatgacgga agaggatttt gagcgcgtct tgaaaatcaa
     5101 tttgaccggt gcttttaaca tgactcaatc agtgttaaaa ccaatgatca aggcccgcca
     5161 aggagcaatt attaatgtct ctagtgtggt tggtttaaca ggcaatattg ggcaagcaaa
     5221 ctatgctgct tctaaggcag gaatgattgg ttttaccaag tctgttgccc gtgaagttgc
     5281 tgctcgtaac atttgcgtga atgctattgc tccaggattt atcgagtccg acatgacagg
     5341 tgtccttcct gaaaaaatgc aagagcaaat cttaagtcaa atcccaatga aacgtattgg
     5401 aaaagctcaa gaagttgctc acttagcaag cttcttggtg gaacaagatt atattacggg
     5461 acaagttatc gctattgatg gcggtatgac aatgcaataa ggagacgtag tgaaatgaca
     5521 tttaaaagag tagtggtaac aggttatggt ctaacatctc caatcgggca tgatcctgaa
     5581 acgttttgga acaatctgaa agctggccaa attggtattg gtcctattac taaatttgac
     5641 acgactgatt atgcagtcaa gaatgctgct gaaattcagg attttccatt tgacaaatac
     5701 tttgtgaaaa aagatttgaa ccgtttcgat agatattctc tttatgctct ttacgcagcg
     5761 aaagaggcta ttaatcatgc tgatttaaat attgagatgg ttgattccga tcgatttggc
     5821 gttattgttg cttcaggtat tggtggaatt gcggagattg aagagcaggt gattcgctta
     5881 catgaaaaag ggccaaaacg tgtgaagcca atgacgttgc caaaagcgct accaaatatg
     5941 gctgcaggaa atgtcgccat gtctttaaaa gctcaaggag tatgtaaatc aattaatact
     6001 gcctgcgctt cttcaaatga tgcgattggg gatgcctttc gtgccattaa atttgggact
     6061 caagatgtga tgatagttgg tgggtcagag gcggccataa ccaagtttgc cattgctggc
     6121 ttccaaagtt taacagctct ctcaacaact gaagatccaa gcagaagctc tattccattt
     6181 gataaagacc gaaatggttt tatcatggga gaaggatcag gaatgcttgt tttagaaagc
     6241 ctagaacatg cgcaagaacg cggggcgaca atcttagcag aaattgtggg ttatggaaat
     6301 acctgcgatg cctaccatat gacatctcca aatcctgagg gattaggagc tcgtaaagcc
     6361 atccacttag ccttacaaga agctggtatt gaagccagtg ctattaatta tgtcaatgcg
     6421 cacggaactt caactccagc taatgaaaaa ggagagagtc aagcaattgt agccgttctt
     6481 ggtaaggatg ttccggtgtc ttcaacaaaa tcgtttacgg gtcaccttct tggagcagca
     6541 ggcgctattg aagctattgc cactatcgag gctatgcgtc ataactatgt tccgatgacg
     6601 gctgggacac aagctttatc agaggatatc gaagcaaatg tcatctttgg agaaggaaaa
     6661 gaaacagcta tcaactatgc catttcaaat acttttggat ttggtggtca taatgctgta
     6721 ttagctttca aatgttggga ggagtaggtt gaatattcaa gaaatcaaag atttgatggc
     6781 tcagtttgat acctcaagcc tacgtgaatt tttattcaaa acaaatgaag gtgagctaat
     6841 ctttagtaaa aacgaacaac acctcaatgc atcgacatca aatcaagaac atgctgtgcc
     6901 tgtaccacag gtacaacttg ttccgaattc aactgcttcg gaagcgtcca gtccagcatc
     6961 agttaaggat gttcctgtag aggagcaacc acaagcagaa tcttttgtag cagaaggtga
     7021 tattgttgaa agtcctttag tcggtgtagc ctatcttgca gctagccctg acaagccacc
     7081 atttgtggca gtcggagata ctgtaaaaaa aggacaaacc cttgtgatta tcgaagccat
     7141 gaaggtgatg aacgaagtgc cagcgccatg tgatggtgtg attacagaaa ttttggtatc
     7201 aaatgaagat gtcattgaat ttggacaagg attggtgcgg atcaaatgat ggatattaga
     7261 gaaattcaag cggctttgcc tcatcgctac ccaatgttat tggttgatcg tgtgttagaa
     7321 gtgtcggacg accatatcgt tgccattaaa aatgtgacta ttaatgaacc tttctttaac
     7381 gggcattttc ctcactatcc tgtgatgcca ggtgttctga ttatggaagc tttggctcaa
     7441 acagctggtg tcttagaatt gtcaaaagaa gaaaataaag gcaagttggt cttttacgct
     7501 ggaatggata aagtgaagtt caaaaaacaa gtcgtccctg gtgatcaact tgtgatgaca
     7561 gcaaccttta tcaagcgtcg cggtaccatt gctgtcgttg aagccagggc agaagttgat
     7621 ggcaagctag cagcaagtgg aacattaacc tttgcctgtg ggcaatgaga atgattgttc
     7681 tgcggaggaa gaaaccctta tgtttaaaaa aatcttaatt gctaatcgtg gtgaaattgc
     7741 agtgcgtatc attcgtgctg cacgagaatt agggatttcc acagttgcag tttattcgga
     7801 agcggataag gaagctctgc acacgatatt ggcggacgag gctatctgta ttggtcctgc
     7861 ccgttccaag gaatcctatc ttaatatgaa ttccgtcttg tcagcagcta ttgtgacagg
     7921 agcccaagcg attcacccag ggtttggctt tttaagtgaa aactcaaagt ttgcgaccat
     7981 gtgtgaagag atgaatatta aatttatcgg tccatccgca tcggttatgg ataaaatggg
     8041 tgataaaatc aatgctcgtt ctgaaatgat taaggcagga gtgcccgtta ttccagggtc
     8101 agatggtgaa gtttataacg ctcaagaagc cttagctatt gccaataaga ttggttatcc
     8161 tgtcatgcta aaagcatcgg caggtggtgg aggtaaaggg attcgtaaag tggagacaga
     8221 agcagatttg gaggcggctt tcaatgctgc cagtcaagaa gctttgggag ctttcggaaa
     8281 tggcgccatg tatttggaaa aagtaattta cccagctaga catattgagg tgcaaatttt
     8341 aggagacgct cacggtaaca tcattcactt gggagaacgg gattgctcct tgcaacggaa
     8401 taatcaaaaa gtcttggaag aaagtccatc cattgctatt ggcaataccc tcagacacga
     8461 aatggggcaa gcagcggtta gagcagctga agctgtagcc tatgaaaatg cgggaaccat
     8521 tgagtttttg ttggacgaag acagcgagaa attctatttc atggaaatga atacccgtat
     8581 ccaagttgag catcctgtca ccgaatttgt gacaggagtg gatattgtca aagagcaaat
     8641 caaaattgca gcaggtcagc ctttggcgat tagtcaagaa gacatcacta tcacgggaca
     8701 tgctattgaa tgtcgtatca atgctgaaaa tactgctttt aactttgcac cgagtccagg
     8761 aaagattaca gacttgtata tgccaagtgg tggtgtagga ctacgagttg attcagcagt
     8821 ttacaatggc tatgccattc ctccttatta tgacagcatg attgctaaaa tcatcgttca
     8881 tggaagcaat cgatttgacg ctttgatgaa aatgcaacgt gccttagttg aattggaaat
     8941 tgaaggcatt atcaccaata cagacttcca gctcgatttg atttcagata agcgagtgat
     9001 tgcaggagat tatgatacct ctttcttgat ggaaacgttc ctgccacatt acaataaaga
     9061 ttaggagaaa atatggcttt atttcgtaaa aaagataaat acatccgtat tactcccaat
     9121 aattccctca aaggatctgt cagccataat gtcccagaag ttccagatga actctttgcg
     9181 aaatgcccag cttgtaagca catgatttat aagaaagatt tgggattggc aaagatttgc
     9241 ccaacctgtt cttacaattt taggatttct gcccaagagc ggctgacctt aacagttgat
     9301 gaaggctctt tccaggaatt atttacaagt attgaaacca aggatccgtt acgctttcca
     9361 ggttatcagg aaaaattaca aaaagccaag gaaacaactg gtctgcatga agctgttttg
     9421 acagggaaag ctatggttaa aggacaacaa attgctcttg ctattatgga ttctcacttt
     9481 attatggcta gtatgggaac tgttgttggg gaaaaaatta cccgattgtt tgaattggct
     9541 atcgaagaaa acctgccggt ggtgattttt acggcttcag gaggcgcccg tatgcaagag
     9601 gggatcatga gccttatgca aatggccaag gtctctgcag ctgtgaaaag acattctaat
     9661 gcaggcctct tttacctcac tattttgaca gatccaacta ctggtggggt aacagctagt
     9721 tttgccatgg aaggggatat tattttggca gaacctcaat ccttggttgg ctttgcaggt
     9781 cgtcgtgtca ttgagaccac agttcgtgaa aacttgccag atgatttcca aaaagcagaa
     9841 tttttacaag accatggttt tgtggatgct attgtgaaac gaacagagtt gcgagacaag
     9901 attgcccact tagtagcatt tcatggaggt ggccaatgac agatgtatca agaattttaa
     9961 aagaagcgcg tgatcaaggg cgtttaacaa ctttggatta cgccaacctt attttcgatg
    10021 actttatgga actgcatggc gatcgccatt tttcagatga tggtgctatt gtaggtggct
    10081 tagcttattt ggcgggacaa cctgttacgg tcattggtat tcaaaaaggt aagaatttac
    10141 aggataattt ggcaaggaat tttggccagc ccaatccaga aggttatcgt aaagctttgc
    10201 gccttatgaa acaggcagaa aaatttggac gaccagttgt tacgtttatc aatactgcag
    10261 gagcctatcc aggtgtcggt gcggaagaac gaggacaggg tgaggccatt gctaaaaatt
    10321 tgatggaaat gagtgatctc aaggttccca ttatcgccat cattattggt gaaggaggct
    10381 ctggtggtgc attagcctta gcggttgccg atcaggtctg gatgctagaa aacaccatgt
    10441 atgcagtcct tagtccagaa ggctttgctt ctattttatg gaaagatggt tcaagagcga
    10501 cagaggctgc tgaattgatg aaaattaccg caggcgaatt gtatcaaatg ggcatagtag
    10561 accgtattat tccagaacat ggttattttt caagtgaaat cgttgacatc atcaaagcta
    10621 acctcatcga acaaataacc agtttgcaag ctaagccatt agaccaatta ttagatgagc
    10681 gttaccaacg ctttcgtaaa tattaacaac atccttttgg agtctcccta cgtgtttatc
    10741 aagctaaggg aggcctttcc aaaagggttt ttaactagtc ttgtaaataa ggaaatgggt
    10801 atactaataa gacttttgat aaactagtgt gaaaaaaagt gttgttaaag gggcaatgta
    10861 gtgagtttca agtgatgtgg tggctcttta aaatagtaaa ccccagaaat cactttaatg
    10921 atctctgggg tgagtttatt atttaggtga aatgacggtt tcgccaccca tgtaagagcg
    10981 gagtacttca gggatggtaa cagaaccatc ttcattttgg tagttttcta aaatggctgc
    11041 tacggtacga ccgacagcaa gtcctgaccc attaagggtg tgaagaagtt tgaccttacc
    11101 gtcagcttca tcacggtaac ggatttgagc acgtcgtgct tggaaatctt cagtatttga
    11161 acatgatgaa atttcacggt aggtgttttg tgcaggaatc cagacttcaa ggtcataggt
    11221 tttagcagct gagaagccca tgtctccagt acaaagggaa atgacgcgat atggcaaacc
    11281 aagtttttga agaatatttt cagcattagc agtcattttt tctagttctt ggtaagattc
    11341 ttctggttta gcaaatttaa ccatttcaac tttgtggaat tggtgtaggc ggataaggcc
    11401 acgtgtgtca cgacccgcag aacccgcttc agaacgaaaa gatgggctca ttgcagtaaa
    11461 gtaaattggc aattctttgc cgtctaggat ttccccacgg taataattgg tcaaaggcac
    11521 ttcagcagtt ggaatcagga caaagttggt atctgctaat tcaaaggtgt cctctttgaa
    11581 ctttggatat tgtcctgtac caaacatgga atcgtggtta accatgtaag gtgtgatgat
    11641 ttcttgataa ccttctttga tatgttcatc cagcatgaag ttataaagag cacgctccaa
    11701 acgagcacca agatttttat agaataagaa acgagcacca gttacctttg ccccacgttc
    11761 ccagtctaaa atatcaagat cttcgcctag atcccaatga gccttaactt cgaagtcaaa
    11821 gtcacgaggc gttccccaac ggcgaatttc gacattgtct tcttcgtcag ctccaacagg
    11881 gacagaatcg tgaggagtat taggtaaaac agtgatgatg tcagtaactt gctggtcgat
    11941 agctactagt tgattgtcaa tagttttgat atctgcagag actttttgca tatcagcaat
    12001 ttgttgagtg gcatcttctt tttgacgttt tgcttgggcg atggctgcag aagcaatatt
    12061 acgctctgct tttaattctt ctgattgcac taatagagca cgacgttttt catcaagttc
    12121 tttaagatgg gtcagggtgt cttcagaaac accgcggttt tttaatttgg cagcgacagt
    12181 gtcaaaatcg gtacggatac gtttaagatc tagcatacag aagctccttt aagatagtta
    12241 ttagtagctg tcagtgaaac aaggctaagt aagacagttg gatcttgtga ttctctgacg
    12301 atagaaaaag cacagcttat gaagatgctg gagcgctttg gcgcggttcc atccagcttc
    12361 acaatctgtg cacttaatct ctattttatt gatgacacgg tagaatttca tatcattttc
    12421 cctatctgtt cacagcatcc acagactctc tgaatgaaaa aaaggtatta cttatccgtt
    12481 aggactatta taacaaaaaa caagaaaaag taaactcaaa tgagggaatc ttttccacta
    12541 aatagcaatt tagagctact attctttgct ttcttgaaat gataaatcag cactgctcat
    12601 tattttttta atgtcgaaaa aatttgttaa taaaggtttg catcaaggtt ggtaatgtaa
    12661 tcactttctc attaccaata tgttgacgag tgatttttaa gatttttcca gagtctccag
    12721 aagcaaataa aaacttgcct tggtccgtaa aaatttcaaa gtggcggcta actttttttc
    12781 ttgaaacatt agccccgatg tgattgatag ctgtccaagg aatttgaata taatcattca
    12841 catttttatc gttgtaaaat tcaagggcct tgtcaccgag taaaatatga ccaactttgc
    12901 ctcctattcc aaggtaggag acagcttttg tcttgtattc gacactagtg tttaaagatt
    12961 gagccattta agttcctttc attgcgtagt tgtagttatt ataccatatt ttaagtggga
    13021 ttttcctgac ataaagcata aaaaagaagc ctgagttcat ctcagacttc cattggttga
    13081 ttacataata cctgcaaggt gagcaaggat accaaatgca aacaagccga tgatgattgc
    13141 gattggagaa acttttttct tcaataacca catgcaaagg aatgtaagga gtaatccagc
    13201 taaacctggg ataagagagt ccaattgtcc ttgaagggtt tgagcttgga ctttatcaag
    13261 gctcataccg ccaatagcgt tggcaaggat agttttcaac tcagcacctt taacagcgcc
    13321 atctgggaag accacatagg caccatctga caattgtttt ccaggaaggt cgatagtaaa
    13381 gttgatagat acccaacgtt gaacaagcac tgcaaggata aacattccaa ggattgaagc
    13441 acctttagta atgtcttgga ggataccgcc tgacatgtct ttagtaattt cagaaccagc
    13501 tttgtaacca aattcttgag tataccataa gaaggccata cggataaggt tccatccaaa
    13561 gaagaacaga agtggaccaa cgatgttacc tgttgaagcc aaagaagcac caagtgcccc
    13621 aaggatagga cgaaccgtaa accagaatac tgggtcaccg ataccagcaa gaggtcccat
    13681 cataccgatt ttaacccctt ggatagcctt gtcatcaatt ggcgtaccat ttgcgcgttc
    13741 ttcttcaagg gcaagtgtta caccaatgat tggagctgca acgtatgggt gagtgttaaa
    13801 gaattccatg tggcgctcaa gagcagctgc acggtcttca ggagacgtat acaatttttt
    13861 aagagcaggg ataagtgcat aagcccagcc catgttttgc atacgttcgt agttccaaga
    13921 accttgtaag aattgtgaac gccaccaaac acgttgacgg tctgatttag ttaatttaat
    13981 ttgttcagtc atttgttctt acccctttct agtagtcttc taagatgtcg ccgattggat
    14041 cagctgaacc tgctgcatta ccaccgttac cacctttttt agaaagatta aggtagatga
    14101 aggcaattgc tacaccgata acacccatag cgataagggt taattggctg atagctgcaa
    14161 gagcaaaacc tagggcgaag aatggccata cttcacgagt agccatcatg ttgataacca
    14221 tagcataacc tacagcaaca accatcgcac caccgatttg cataccttcg ttcaaccagt
    14281 ttggcatagc attcaaaaca gattgtacgg ctgaagtagg aacagcaaca agaagagctg
    14341 ctgggacagc gatacgcaat ccttgaagaa gaagggcaat aaagtggaag cgttcaatac
    14401 ctgcgaaatt accttcagtg gcagctttgt caccagcgtg tacaagggca gttgacaaag
    14461 tacgaacgat cattgtaagg aaaagaccag caacagcaag tgggatagct gttgagtaag
    14521 caaaggtaat tcctttttga gtaaagtcgc cacttttgac catgatgata gcagctgcaa
    14581 cagaggcaag ggcagcatca ggggcaacgg cagcaccgat gtttgcccaa ccaagagcaa
    14641 gcatttgaag tgtaccacca aggatgacac cagcttcaag gtgtccagtt acaagtccaa
    14701 tcaaagtaca ggcaacaagt ggttggtgca tttgaaattg gtcgaggata ccttcaagac
    14761 cagcgaagaa ggcaatgata acgactaaaa ttgcagaaat aatcgaaata tctgacatgt
    14821 tgtaatcctt tcaaagtata gtaaacgagg actttttgct aataagttag ctagtcatcg
    14881 ttttaaaacc ttatagcgtt tgaaacttat ttaatgttag ctttttggat aagctcaaat
    14941 aagtttttct tagaatcgtt aggaacttta cggacgtcga atgtaacacc taggtcgcgt
    15001 aatttttcaa aagtagcaac gtcttctttg tccattgata aaacgttgtt aaccattgtt
    15061 ttaccagttg agtgagccat tgaaccaacg ttcaattcgt tgatttcaac accaccttca
    15121 accgcgcgaa gggcatcttg aggggtttgg aaaagaataa gtgcgtgtgt gttaccaaaa
    15181 cgaggatctt tagaagcttc aatcaatttg ctaattggaa caacgttagc tttgacacca
    15241 ccaggtgcag cctgtttgat caattgtttg cgaaggtcat cttgagcaac ctcatcagaa
    15301 gcaacaatga tacggtcagc cttagaagct ggagtccatg ctgttgcaac ttgaccgtgt
    15361 aaaagacgag tgtcaacacg agcaagattg atctttaatt taccatctcc gatgacagtt
    15421 ccagcaggaa tagctccttg aagtgcgtta acaacttttt cagttgcagc agtttcttca
    15481 actgggttaa ggtcttcagg aagggctttg ataccgtcct tagattcctt aatgatgttt
    15541 gcagcaactt gttcaacacc tgcaccagca tccataagac gttcagtgta agcttgaata
    15601 agcataggca agttgagacc agtgatgatc gccattttgc ggtcaggatt ttcacctgcc
    15661 acacgactag cttggttaaa tggagaacca ctccaaaggt cagctagcac aaggatttca
    15721 tcatcagcat caaattgttg gatagcattg ttgaagtgtc catacaaatc atctggtcct
    15781 tcgtttggca tgaaagtaac aacttgaact ttctcttgtt caccaaaaat catagaacca
    15841 gattgatgaa taccttcagc aaatttacca tggctggcaa taataatacc gatacccatt
    15901 cttgttcttc ctcctttaaa atatttttcg ttgaaatgaa gaagaataat ctccttccaa
    15961 aactaaattt tggcaattct ttttcactta aacgaaattc aagaacgttt ctattttaaa
    16021 acgttttcac taaaaaatca agccctttcg gaaaaatagt acaaaacatg aaaaaatttt
    16081 cagaatttag gattaaatag gcagattatt gatattaaag gtgttttcaa gctgtttaaa
    16141 acaaaaatat gctgaaaaat gttgaaatta ttaaaatgat tttcattttt tgatagctgt
    16201 tgacagaaga aaaaccacca ttttctgaaa aatagtggtt tcgtttgcta ctacaagaaa
    16261 agttcttcca attttttggc aacaccgtct tcttcgttgg aaaaattaag ctgctgatca
    16321 gcataaggca gtagcacagg gctagcattt ttcatagcat aaccagttcc agcaaaagct
    16381 agcatctcag tgtcattgtg ttcatcacca aaggcgatta aatccttttt atccatattg
    16441 taaattccaa ggagatagtt cagagcgtaa gctttattga cattcttaga cgaaatttca
    16501 agaatgttga gagggcctcc ccaagaatca atttcaattt catctttgaa gaaagcacgc
    16561 atgtttttag ctaaggcata cttgtcttcg tgatgcgttt gcatcaataa ggcgtttgga
    16621 tttcgagtga ttttggtaat ctctaatgcc atgtcctgag taatttcatc aacaccaaag
    16681 agttgagggt cgatactttc tggattggtc atggtgatgt agacattttt tcgatactca
    16741 ctggcaataa aatccatctg gaaatcatcc tgatatttta gtaaccttag taggtagcct
    16801 ttgtccaaag tgacattatg ttcataggcc catttttgtt caggcatgtg ggtcaaggcc
    16861 ccattaaaag taatgattgg ggtctttaaa ttgagctgca gatagtagtc cagtgccatg
    16921 cggtaaggac ggcctgttga aataatgacg tggtggccct tgtcttggac agctttgatg
    16981 attttttgag tataggtaga gatggtgttg tcatgatgta ataaggttcc atccaaatca
    17041 atggcaatca attttttagt cataattaaa ttatagcaaa ttttgctcca agataaagag
    17101 aaaagataag ttaagcgtat tcattagtaa aaaagatagg ataactgttt ttttaatgaa
    17161 taaatgaagt tacagacgtt gaaaagtgaa caaaaaaatg gtaacatagt gttattgttc
    17221 attttaatac gaacaataac aataataatg aggtgtcaaa aagacttatc tagtgataag
    17281 cgctttttga ttttacctac tatttaatta aggagactcc atttcaatgg aaaagttttt
    17341 taagttaagc gaaaatggga caactgtctc aactgagatt atggctggtt taacgacctt
    17401 ttttgccatg tcctatattt tgtttgttaa cccaagtatt ttaggtgcag cggggatgcc
    17461 ctctaatgct gtctttttgg ctacgattat cgcagcagcc atatcaacct taattatggg
    17521 actatttgcc aatgtgcctt atgcgttggc accaggaatg ggacttaacg cttttttcac
    17581 ttatacagtt gtttttgctt tagggttttc atggcaagaa gccttggcaa tggttttcat
    17641 ttgtggatta ttcaatattt ttattaccgt aaccaagttt cgtaaaagta tcatcaaggc
    17701 gattccagtt agtttacagc atgctattgg tgggggaatt ggtgtctttg tagcttattt
    17761 aggatttaaa aacgcaaata tcattacttt ttctatctct gctgaaaata tagtaatggt
    17821 aaatggtgtt gaaccggcta aagcatcggc taaaacattt gcagatggtc tattatttgt
    17881 agacgccaat ggtggagttg tacctacgat ttctagtttt acggattcta gtgtattact
    17941 tgctattttt ggtttacttt taacgacagc tcttgtgatt cgaaatttta gaggtgctat
    18001 tttaattggt attgtcgcaa caactcttgt aggtattcct ttaggaatag tggatgtgtc
    18061 caacctcaat tttgggatca gccatattgg tgaagcttgg actgaattag gtacaacttt
    18121 ccttgcagct ttcgatggtt tgagttctct ttttagcgat tcaagtcgtt taccgctagt
    18181 tttcatgact atttttgctt ttagtctatc agatactttt gacacaattg gtacctttat
    18241 cggaactggt cgtcgaacag gtattttctc tcaagacgat gagaatgctt tggaaaatag
    18301 tataggcttt agttcaaaaa tggatcgtgc gctttttgca gatgctatcg gtacttctat
    18361 tggggctttg gttggaactt caaatacgac tacctatgtt gaatcagcag caggaattgc
    18421 tgaaggtgga cgtactggac taacagcagt ctccaccgca gtatgcttct tattatcaat
    18481 attgctatta ccgcttgtag gtattgtccc agctgctgct acggctccag ctttaattgt
    18541 tgtgggtgtc atgatggtgt cttcttttct tgatgttaat tggagtaaat ttgcagatgc
    18601 tcttccagct ttttttgcag ctttctttat ggcgctgtgt tactctattt cctatggtat
    18661 tgccgctgcc tttattttct attgtctagt aaaagttgtt gagggaaaaa caaaagatat
    18721 tcaccctatt atttggggag caaccttctt gttcattgta aatttcatca tattaactat
    18781 cttataacca aataatttac ttattttgct actaactgcc gcttctttaa ttgattttaa
    18841 cctttaatat aacgaagcgt ttttacttta cgaatacctt attggagtca tcttctaggt
    18901 agtgcttgga agtatgtttt ttttctgata taatgttgct atgttttata gtgaaaatga
    18961 atatactctt aaagcttacg gagaaaccct aggtacttat ttgtctattg gagatgtgat
    19021 tgtattgtct ggcgacttag gtgccggtaa aacaacattg gcaaaaggta ttgctaaagg
    19081 gatggggatt agtcaaatga tcaaaagccc aacttatact attgttagag aatatgaagg
    19141 acgtctacct ctttatcact tagatattta ccgtgttggt gatgatccag actctattga
    19201 tttagatgat tttctttttg gtaatggtgt taccgttatt gagtggggag aattattagg
    19261 agagggatta ttacaagact atttgcaaat aaccattact aaacgagata aaggtcgtca
    19321 actagacttg ttagcgcatg gtgagcgctc tcgtcagctt ctggagataa tgtcacatga
    19381 ttcagcaaag tctcgttatt gaagaaggcg tacctcaaga tgcaaaagct gtactttcct
    19441 tagtggcggc agctgccaaa gaaacagatt ttataacagg cgtagagact atattagagg
    19501 ctactcctca tgaattatca gactttttat caagaagtca aacctctttt attgattttt
    19561 gcctgctagc tagattagat gaaaaagtag tagggctttt aaatctttct ggggaagtat
    19621 tatcccaagg ccaagttgaa gcagatgtat ttatgttagt tgctaagact taccgtggtt
    19681 atggtatcgg tcaattattg ttagagattg ctcttgattg ggctgaagaa aacccttata
    19741 tcgaaagcct taagttggac gttcaagtaa gaaatactaa ggctatttac ctgtataaaa
    19801 agtatggttt tcgtattgaa agtatgcgaa aaaacgacat caaatcaaaa aatggagatg
    19861 acttagatgt ttatcatatg cgtaaatctg taagtcagta agtgaatcga tgaaaattgg
    19921 aaaaaaaata gttttaatgt tcacagctat tgtgttaaca actgtcttgg cattaggtgt
    19981 ctatctaact agtgcttata ccttctcaac aggagaatta tcaaagacct ttaaagattt
    20041 ttcgacatct tcaaagaaaa gtgatgccat taaacaaaca agagcttttt ctatcttgtt
    20101 gatgggtgtt gatacaggct cttcagagcg tgcctccaag tgggaaggaa acagtgattc
    20161 gatgattttg gttacggtta atccaaagac caagaaaaca actatgacta gtttagaacg
    20221 agatacctta accacgttat ctggacccaa aaataatgaa atgaatggtg ttgaagctaa
    20281 gcttaacgct gcttatgcag caggtggcgc tcagatggct attatgaccg tgcaagatct
    20341 tttgaatatc accattgata actatgttca aattaatatg caaggcctta ttgatcttgt
    20401 gaatgcagtt ggagggatta cagttacaaa tgagtttgat tttcctatct cgattgctga
    20461 aaacgaacct gaatatcaag ctactgttgc gcctggaaca cacaaaatta acggtgaaca
    20521 agctttggtt tatgctcgta tgcgttatga tgatcctgag ggagattatg gtcgacaaaa
    20581 gcgtcaacgt gaagtcattc aaaaggtatt gaaaaaaatc cttgctcttg atagcattag
    20641 ctcttatcgg aagattttat ctgctgtaag tagtaatatg caaacgaata tcgaaatctc
    20701 ttctcgcact atccctagtc tattaggtta tcgtgacgca cttagaacta ttaaaactta
    20761 tcaactaaaa ggagaagatg ccactttatc agatggtgga tcataccaaa ttgttacctc
    20821 taatcatttg ttagaaatcc aaaatcgtat ccgaacagaa ttaggacttc ataaggttag
    20881 tcaattaaaa acaaatgcta ctgtttatga aaatttgtat gggtcaacta agtctcagac
    20941 agtaaacaac aactatgact cttcaggcca ggctccatct tattctgata gtcatagctc
    21001 ttacgctaat tattcaagtg gagtagatac tggctcatct tatcagagtg ctagtacaga
    21061 ccaggactct actgcttcaa gccatacgcc aactacgccg tcttcttcat cagatgcttt
    21121 agcagctgat gagtctagct catcagggtc tggatcatta gttcctcctg ctaatatcaa
    21181 ccctcagacc taacgttctg agggttttta tgtttaaaat atccgatttt gaatataaaa
    21241 aagacaggta tcaaagatga tagctgtctg tgatttcaaa taaagtatat gagggagata
    21301 atactatctg gaatggttgt tagttaaaat gacggtttgg caagtaggtg attcactaga
    21361 aattagctca ctatcttctt gatatttggc catacgattt tggatttcag taaggtgtgc
    21421 ttgtgtctct tggttaaagt agcgccattt atgggccaag tcctgactaa tattttgaat
    21481 tacttttcct tgactgtgaa tgaggtcaag gtttgcttta atgttatcta aatcaagttg
    21541 ggcagcatct tttgcttgtc gactgatgga taacagctct tttatggtac aacgtttttg
    21601 atatgctttg taagcggtaa aagataataa tccgacggct atcacggttt taactttcat
    21661 gaactaacct cctgattgat ttgtcgggcg agcttttcca agacaggaaa gttaggctca
    21721 tgagtggtgt attgaattga gataccgtct ttttcattat aacgaggaac gagatgaaca
    21781 tgagcatgga aaacagtttg tcccgctaag gcttcgttat tgttaatgat attcatggca
    21841 gtagcgccag tagctaactg gatagcacga gcaattttag gaatacgagc aaagagatga
    21901 ctagctgtct cagcagtcat ctctagtaag ttacgaacat gttgcttagg aatgactaga
    21961 gtatgccctt ttgtagtttg tgagatatct aaaaaggcga ggacctgctc atcttcgtag
    22021 actttcgaag aggggatatc cccttgaatg atgctgcaaa aaatacaatt ttccatttta
    22081 tatcaccttc tctacttgaa tttgttataa tatattataa gtatatcaaa aactagaaag
    22141 aaattatatg ttaaacatta aaaatctaac aggaggatat cacaatatcc ctgttttaaa
    22201 cgatgtctca ttttctgtag ataatggaga gttagtcggt ttgatcgggc taaatggtgc
    22261 tggaaagtca acaactatca acgaaattat tggctttttg aagccttatc aaggaagtat
    22321 ttctattgat ggtttaactc tagcagaaaa tgcagttgcc taccgtcaaa aaataggttt
    22381 tatcccagaa actcctagtc tctatgaaga attgacctta tcagagcata tcaacaccgt
    22441 agctatggct tatgatatcg acttagaagt ggcacaaaaa cgagcacaac ctttcttgga
    22501 aatgtttcgt ttaactgaca aacttgaatg gtttcctgtc aatttttcta aagggatgaa
    22561 acaaaaagtc atgattattt gtgcctttgt cattgatcca agtctattta ttcttgatga
    22621 accattctta gggttagacc ctttagctat ttcagactta atccaaacgc tagaagtcga
    22681 aaaagcaaaa ggaaaatcta ttttgatgag tacacatgtt ttggactctg ctgaaagaat
    22741 gtgtgatcgc tttgtgattt tgcatcatgg acaagttcgt gcccaaggaa ctcttgctga
    22801 cttgcaagaa gcttttggtg accgttcagc gagcttaaat gatatctatc ttgcattaac
    22861 aaaagaggat taagtatgaa agcattattt ctgaaacgcc gtcaagactt tcaaaaacaa
    22921 caaaacaaat accttcgtta cgtcttaaat gatcactttg ttttggtgtt gatgttttta
    22981 ttaggctatg ctatggtgca gtatggtcag ctactaaacc attttccaac gaatcatcta
    23041 cctattcagg tttgtcttgg gatattgatc ccattattgc tcagtatggg atctatagca
    23101 acgtatctag aagaggcaga tcagcatttc ttacttccaa aagaagaaga agtgatttct
    23161 tatatcaaac aagctgaacg actttctttt ttgctttggg gaactttgca gactgctata
    23221 ttgctctttt tatatcctat ttttagacgt ctaggtctgt ctttatttat ttttattatc
    23281 ttagttttga tattattggc actaaaaaga gtagtgctta gtcgaaagac aagatatttt
    23341 ttgagaggaa atcgtttaga ttgggcaaaa gctgtcgcat ttgaaagcaa tcgcaagcaa
    23401 agtattttaa agttttattc tctttttaca acagtcaaag gaatttctac aaaagtgaaa
    23461 gaaagaactt atctaaatcc tcttttaaaa ttagttaagc aaactccttc aaacctttgg
    23521 ctatcccttt atgctagagc ctttttacgt agttcagatt atttgggctt atttttacga
    23581 ctaatgctat taagctcatt atctgtattt tttatccata atctttattt gtcagtaagt
    23641 ttagcactca tttttaacta tttagttgta ttccaattac tttcacttta ttatcactac
    23701 gattaccatt atatgactag tctttatcct gaaaatagtc ggagcaagaa gaaaaatgtg
    23761 ttaagttttt tacgaggttt aagcttttta atgttaatcg tcaatatgct gtgttgctca
    23821 tcagcgccaa aagcactcat tttgattgta ggtatggttt tcatagcttg tatatatcta
    23881 ccttataagt taaagaagat cattgactaa gtagcctaaa acaagtaaaa taggggtaaa
    23941 cgactataaa agatagaaaa ggagggcgat aaggtgacaa cgacggaaca agaacttacc
    24001 ttgactccct tacgtgggaa aagtggcaaa gcttataaag gcacttatcc aaatggggaa
    24061 tgtgtcttta taaaattaaa tacgacccct attctacctg ccttagcaaa agaacagatt
    24121 gcgccacagt tactttgggc caaacgcatg ggcaatggtg atatgatgag tgcccaagaa
    24181 tggcttaacg gccgtacatt gaccaaagaa gatatgaaca gtaagcaaat cattcatatt
    24241 ctattgcgcc ttcacaaatc taaaaaatta gtcaatcaac tgcttcagct caattataag
    24301 attgaaaacc catacgattt attggttgat tttgagcaaa atgcaccctt gcaaattcag
    24361 caaaattcat acttacaagc tatcgttaaa gaattaaaac ggagcttacc agagttcaaa
    24421 tcagaagtag caacgattgt gcatggagat attaaacata gcaattgggt gattactact
    24481 agtggtatga tttttttagt agattgggat tctgttcgtc tgactgatcg gatgtatgat
    24541 gttgcttacc tgttgagcca ctatattcca cggtctcgtt ggtcagaatg gctgtcttat
    24601 tatggctata aaaataatga caaggttatg caaaaaatta tttggtatgg tcaattttct
    24661 cacctgacac aaattctcaa gtgttttgac aagcgtgaca tggagcatgt gaatcaggag
    24721 atttatgccc tcagaaaatt tagagaaata tttagaaaga aataatgcga gttagaaaac
    24781 gaaaaggtgc tgaggagcac ttagctaata acccacacta tgtgatatta aatccagaag
    24841 atgccaaggg ccgttggcat gacgtttttg gaaatgaccg tcctattcat atcgaagttg
    24901 gttcaggaaa gggcggattt atcacaggga tggccttgaa aaatcctgat attaactaca
    24961 ttggcattga tattcaatta tctgtcctaa gctacgcctt ggataaggta ttagcttcag
    25021 aagtacccaa tgtcaaactt ctgcgagtgg atggatcaag tttaaccaac tattttgaag
    25081 atggtgaggt ggatatgatg tatctcaact tttcagatcc ttggccaaaa accaagcatg
    25141 aaaaacgtcg tttgacctac aaggatttct tagacactta caagcgtatc ttgccagaac
    25201 acggtgaaat tcattttaaa accgataatc ggggcttgtt tgagtacagt ttagctagtt
    25261 tttctcaata cggcatgacc ttgagacaga tctggcttga cttacacgct agtaactatg
    25321 aaggaaatgt catgacagaa tatgaagaga agttctctaa taaaggtcaa gtcatttacc
    25381 gagttgaggc aaatttctag tagtattttg tttcaaaata tgataacata aaggagttga
    25441 tacagtatta acttctttag taatggagag gtcgcatagt ggcttagtgc gcacgcttgg
    25501 aaagcgtgta gtccttaacg gggctcgggg gttcgaatcc cctcctctcc ttgtgagtaa
    25561 aacaaagtgc atatccttgc attcctctta aattatggta taatgggctt aaataataca
    25621 gaaaggtggt gggatatgtc tcgccgcttt tgtattaagt ggattcccaa ggaccaatta
    25681 tcttagagaa gagcataaaa atagaggagg taatcaaaat agcaaataca tctattatcg
    25741 atatcgtaac caagacagta acacctgaga ttaaggcacc ttatgaatta gtagacgttg
    25801 aatatgacaa aatgggtagt gactacatcc ttagtatttt agtcgataag gaaggtggaa
    25861 tcacagtgga agatacatct gacttgacga atattattag tccgctgtta gatacgattg
    25921 atccagatcc ttttccaaac caatatatgc tagaagtctc tagtccaggt ttggagcgtc
    25981 cgcttaagac agctgacagt ttaaaagcag cagttggctc ttatatcaat gtgagtcttt
    26041 atcaagctat tgataaggtg aaagtttttc aaggagactt actagccttt gatggtgaaa
    26101 cgttgaccat tgattatctt gataaaacac gtcataagat cgtgaatata ccatatcaag
    26161 cagttgctaa ggtgagaatg gctgtcaaat tataactttt atttcaaagc actaaacttt
    26221 tttaggaacg cttaagcaaa tttgaaacaa cacctataga gtttatacaa gctaaacaaa
    26281 aggaattaag ggagccttta gggggctatt gatcgtatta atttaagaaa ggatttttgc
    26341 caagctttac aagcaaggta gataacatta tgagcaaaga aatgctagaa gccttccgta
    26401 ttttggaaga agaaaaacac attgataaag cagatattat tgacgcagtg acagaatcat
    26461 taaagtctgc ctataaacgt cgctatggtc aatcagaatc atgtgttatt gaatttaatg
    26521 aaaaaacagc tgattttcaa gtattcacgg ttcgtgaggt tgttgaagaa gtttttgaca
    26581 gccggcttga gattagtttg aaagatgctc ttgctatcag ttctgcctat gaattgggtg
    26641 ataagattcg ctttgaggaa tctgtcaatg aatttggacg tgtcgcagct caatcagcta
    26701 agcagactat tatggaaaag atgcgccgcc aaatgcgtga agtgatgttc aatgaataca
    26761 aagagcatga aggtgaaatt atgacaggaa cagttgaacg gtttgatcaa cgttttattt
    26821 atgtcaatct tggttcctta gaagcacaat tgtctcacca agaccagatt ccaggtgaaa
    26881 ctttcaaatc acatgaccgt attgacgttt atgtctacaa agttgaaaat aatcctaaag
    26941 gagttaacgt ctttgttagt cgtagccatc cagaattcat caaacgtatt atggagcaag
    27001 aaattcctga agtctttgat ggtactgttg aaatcatgag tgtctctcgt gaagcaggtg
    27061 accgtaccaa ggttgctgtg cgcagtcata atcctaacgt tgatgccatc ggaacaattg
    27121 ttggacgtgg tggaagtaat attaaaaagg tcatcagcaa gttccatcca aaacgtgtag
    27181 atgctaagac ggggcttgaa attccagttg aagaaaatat tgacgtgatt caatgggtag
    27241 atgatccagc tgagttcatc tacaatgcca ttgctccggc tgaagtagac atggtacttt
    27301 ttgacgatga agatcttaaa cgtgctacgg tagtagttcc tgatagcaaa ttatcattag
    27361 ctatcgggcg tcgcggccaa aacgttcgtt tagcggcaca tttgactggc taccgtattg
    27421 atatcaaatc agctagcgaa tatgatcgtc ttgaggcgga aaaagaagca gcaacagttg
    27481 tggaagagcc agttgttgac gatgttatga ctgccgagga agtgtaagaa aagaggtgtt
    27541 tgatgtctaa agtcaaaaaa atacctttac gaaaatcact tgtttctggt gaaattattg
    27601 ctaaacgaga tcttcttagg attgtaaaaa ctaaggatgg tcaagttttc attgatccaa
    27661 ctggtaaaca aaatggtcgt ggtgcttata tcaaattaga caatcaagag gccttaatgg
    27721 ccaagaaaaa acaagttttc aacaggagtt tctcaatgga cattccagaa agtttttacg
    27781 atgacttgat tgcttatgtt gatcacaaaa tcaaaagaag agagttaggt cttgactaat
    27841 ttagaaagat tatctagtct tattggtcta gctcaacggg ctggaaaagt gatttcaggt
    27901 gaagaactag tagtaaaagc tattcaacac cagcaggtaa tattagtatt tttagccaat
    27961 gatgctggtc ctaatgtgac aaaaaaagtg acagataaaa gtaattatta taatgtagaa
    28021 gtctccacag tgttgaatgc actggaatta agtgctgcac taggtaaacc aaggaaggtt
    28081 gctgccgttg cagatgctgg attttcaaag aaaatgagga ctcttatgga atagatgaat
    28141 aggaggacac aaattgtcaa agaaaagatt acatgaaatt gccaaggaaa ttggtaaaag
    28201 tagtaaagag gtggttgaac acgctaaata cttaggatta gatgtgaaaa gccatgcatc
    28261 aagtgttgaa gaagcggatg cgaaaaagat tatttcaagt ttttcaaagg cttctaaacc
    28321 tgatgtgaca gcttctcaaa cagtcaagcc caaagaagtt gctcaaccct ctgtaacagt
    28381 ggtcaaagaa acaggttctg agcatgtaga gaaaacacag gtctctaagc caaagagtcg
    28441 taattttaaa gctgaaagag aggctcgtgc aaaggaacag gctgctcgaa aacaagcaaa
    28501 tggctcttct catcgttctc aagaacgtcg aggaggttat cgccagccca ataatcatca
    28561 aactaacgaa caaggagata agaggattac ccatcgttct caaggtgata caaacgataa
    28621 gcgtattgag cgaaaagcat cgaatgtatc acctagacat gataatcatc agctagttgg
    28681 tgatcgtaac cgttcctttg ctaaggagaa tcacaagaat ggtcgattca caaaccagaa
    28741 aaagcaagga cgacaagagc ctcaatcgaa aagtcctaaa attgatttca aagctcgtgc
    28801 tgctgcctta aaagctgaac aaaatgctga atactcacgc caaagtgaaa cacgttttcg
    28861 tgcacagcaa gaagctaagc gtttagctga gttggcaaga caagaagcta aagaagcggc
    28921 tttaaaagca caagctgagg agatgagcca tcgtgaagca gcactgaaat ctattgaaga
    28981 agcagaaact aaattaaagt catcaaatat tagtgctaaa tctactgctg ataatcgccg
    29041 taaaaaacaa gcacgtcctg aaaagaatcg tgagttaact catcattcac aagaaggaca
    29101 aaagaaaaat aagaagagtt ggaatagtca aaaccaagtg agaaatcaaa aaaatagtaa
    29161 ctggaataaa aataaaaaga ccaaaaaagg caaaaatgtt aagaatacta acaccgctcc
    29221 aaaaccagta accgagcgta aattccatga attgccaaaa gaatttgaat atactgaagg
    29281 catgacagtt gccgaaatcg caaaacgtat caaacgcgaa cccgctgaaa ttgttaaaaa
    29341 gctgtttatg atgggtgtta tggcaactca aaaccagtct ttagatgggg atactatcga
    29401 attattgatg gtagattatg gtattgaggc taaggctaag gtagaagtag atgatgctga
    29461 tatcgagcgc ttctttgagg atgaaaatta cctcaatcct gaaaatattg tagaacgcgc
    29521 acctgttgta actatcatgg gacacgtaga ccacggtaag acaactttgc ttgatacttt
    29581 gcgtaactca cgtgtggcaa caggagaagc tggaggaatt actcagcata ttggtgctta
    29641 ccagattgaa gaagctggca agaaaattac cttcttggat actcccggac acgcggcctt
    29701 tacaagcatg cgcgcacgtg gagcttccgt taccgatatc accattttga ttgttgcagc
    29761 tgatgatggt gttatgccac aaacgattga agcgatcaat cactcaaaag ctgctggtgt
    29821 tccaattatt gttgctatca acaaaattga taaaccaggt gctaatccag aacgtgttat
    29881 cgcagaattg gcagaatatg gtatcatctc aactgcttgg ggcggagagt gcgaatttgt
    29941 agagatatct gctaaattta ataaaaatat tgatgaacta ttagaaacag tccttttggt
    30001 tgctgaagta gaagaattga aagctgatcc gacggttcgt gctattggta ctgtgattga
    30061 agcacgctta gataaaggta aaggtgctat cgctacgctt cttgtccaac aaggaactct
    30121 tcatgttcaa gacccaatcg ttgttggtaa tacctttggt cgtgtccgtg ccatggtaaa
    30181 tgaccttggg cgtcgtgtta aatctgcgga gccatcaaca ccagtttcca ttactggttt
    30241 gaacgaaaca ccaatggctg gtgatcactt tgctgtttac gctgatgaaa aagctgcgcg
    30301 tgcagctggt gaggagcgat caaaacgtgc cctcctcaaa caacgtcaaa atacacagcg
    30361 tgttagcctt gataatctct ttgataccct taaagcagga gaaatcaaga ctgttaatgt
    30421 catcatcaaa gcagacgttc aaggttctgt tgaagccctt gcagcatctc ttgtaaagat
    30481 tgaagtggaa ggtgtccgtg tcaatgtcgt tcactctgct gtaggtgcta tcaatgagtc
    30541 tgatgtgacc cttgctgaag catcaaatgc tgttattatc ggtttcaacg ttcgccctac
    30601 cccacaagct cgtcaacaag cagatacgga tgatgtggaa attcgtcttc atagcattat
    30661 ctataaagtc attgaagaag tagaagaagc catgaaaggg aaacttgacc ctgtttacca
    30721 agaaaaaatt cttggtgaag ccattattcg tgaaaccttc aaagtttcta aagtgggaac
    30781 aattggtggc tttatggtaa tcaacggtaa ggtgacacgt gattcgagtg ttcgtgttat
    30841 ccgtgatagt gtcgttattt ttgatggtaa actggccagc ttgaaacatt acaaagatga
    30901 cgttaaagaa gtcggtaatg ctcaagaagg tgggttgatg attgaaaact ttaacgatct
    30961 taaagttgac gatactattg aagcctatat tatggaagaa attgtgagaa aataggggct
    31021 aagaaggtct gagatgtgac gtttttcctc tgtcggaaat aatgacttgt tctaccagat
    31081 gagctacttg gggcattcct tccagtctcg tcatggaatt ctcagattcc ctttagttgt
    31141 cgttatcaaa acaggtagca atgtcataag ctgggctttc agcctagctt ttgttttaaa
    31201 aatgtggaaa ggaaatggct atggctaatc atcgtattga ccgcgttggg atggaaatca
    31261 agcgcgaagt aaatgatatt cttcagaaaa aagtgcgtga tccacgcgtt cagggagtaa
    31321 cgattacaga agtgcaaatg cagggtgact tgtcacttgc taaagtttat tacactatta
    31381 tgagtgactt ggcatctgat aatcaaaaag cacaaacagg gcttgaaaaa gcgactggaa
    31441 cgattaaacg tgaacttggc aaacaattga ccatgtacaa aattccagat ttggtgtttg
    31501 aaaaagacaa ttccatcgcc tatggtaata aaatagatca actgttacgt gagcttgata
    31561 aaaaagatta aattaaaaac ctttaaggta tcttattaga gtcaccttaa gggttttttg
    31621 tttttagtaa gctttttgaa tcgtatcaat gatttgatat tgtgcagtct tggcttcttc
    31681 aattggatag attgggtaaa catgattcat tttatcagcg acgataagtt catgttgatg
    31741 acctttggca gttaatatat catcaaattt cttaacatct gggtagaaaa tttcatgtgt
    31801 accaacaaac atagtaatag gtgctagccc tgatacatct ccattaattg gactaacata
    31861 tggatgtttg gtgttgtttg ggtcacggcg tgcccaaatt tccccaactt tacgtaggcc
    31921 ccaagctgaa agaatgggat cattactttc atagctctca atatctggat ttgacatggt
    31981 tacatctaac catggtgata agagaacaat gtcttttggt tgttttaaac tttcattttt
    32041 tagggctaat gctaaaccaa gcgctagtcc accacctgct gaatcaccca taatagtgat
    32101 attttgtgca gttgtttctt taagagtatc tctgtaaagg tcaaccaatt ttggcatagc
    32161 atcactgtaa gagtaagtag gtgcttttgg atagattggt aagataatct tagcatcaag
    32221 actagttgat aaggttttta gcatgtcgat atgataagta gttgggttgt ttaggtaaga
    32281 gcccccatgt aaatagaaaa tgaccttttg attggcttta ttgctggggt tctcattcca
    32341 agtatgaact tgcatattat caacagtttt agtgactaat gtgccattta cctgttcttc
    32401 tttaagatca aagcctttat ttgaagcttc tccagcagag ttgacatatt gatcaaactt
    32461 agtttcgtct tgctctgaat aatagccttt gtctcctttt aaccaaaagt atttctctat
    32521 aaaccagctt ttccaagatc tagaactggc atcaacttga gtgctagctc ctattgctag
    32581 agtaagtcct agtaaaataa gagtgctttt aagtgtcttt ttcaatgagt tctccttatt
    32641 attatcattt ttttatcata taatc
//
LOCUS       DATXQK010000017        30234 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.017, whole genome shotgun sequence.
ACCESSION   DATXQK010000017 DATXQK010000000
VERSION     DATXQK010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30234
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.017"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(173..2383)
                     /locus_tag="VC394_000869"
     CDS             complement(173..2383)
                     /locus_tag="VC394_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEP5994347.1"
                     /translation="MTSLGKNDKEVATVKKFKTLITGFINTRLGFIITLLFCYWIKTL
                     WAYHTDFSLDLGNIYQVFLTIINPIPLAFLLLGVALYVKNTRAFYICSWVVYIILNIL
                     LISNSIYYREFSDFITVSAMLASSKVSAGLGDSALNLLRIWDIIYILDFIILISLSIT
                     KKIKNDQRPFNKRAAFAITALSSLLLSINLFLAEIDRPELLTRGFSNTYIVRALGLPA
                     FTLYSGNQTYQAQKERNGATAEELIDVKTYVKGHYAAPDPQYFGIGKGKNIIVLHLES
                     FQQFLIDYKLKEGDKEYEVTPFINSLYHSNATLAFPNFFHQVKAGKTSDAETMMENSL
                     FGLNSGSFMVNYGGENTQFATPSILTQKGGYTSAVFHGNVGTFWNRNNAYKQWGYNYF
                     FDSSYFSKQNSKNSFQYGLNDKYMFKDSIKYLEQMQQPFYTKFITVSNHYPYTSLKGE
                     SSEEGFPLAKTDDETINGYFATANYLDAALKSFFDYLKATGLYENSIFVLYGDHYGIS
                     NSRNSSLAPLLGKDSETWSEYDNAMLQRVPYMIHIPGYTNGSIKETFGGEIDALPTLL
                     HILGIDTSQFVQLGQDLLSPQNSQIVAQRTSGTYMTPEYTNYSGRLYNTQTGLEITNP
                     DEMTIAKTKEIRSAVAQQLAASDAIQTGDLLRFDTQNGLKAIDPNQFIYTKQLKQLKD
                     ISAKLGSESTSLYSKNGHKSTQKLFKAPSYLELNPVEADAATSELKEDNPKNKE"
     gene            2491..3654
                     /locus_tag="VC394_000870"
     CDS             2491..3654
                     /locus_tag="VC394_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEP5994348.1"
                     /translation="MNKLYIDSFVEKKLTAGVQLLDEKDFSNIKEKNQLVQLVTKSNR
                     PIGTAYISKQNKGIGWYLGPEKIDLSISYFVSLFSVAKAKRQDFAQSDETNAYRLFNQ
                     EGDGFGGVTIDLYKDFAVFSWYNAFVYDKKEMIMEAFQQVFPEVKGAYEKCRFKGPDT
                     ETAHLYGELAQETFSILENGIAYQVFLNEGLMTGVFLDQHDVRRALVDGLAMGKSLLN
                     LFSYTAAFSVAAAMGGAIETTSVDLAKRSRELSLAHFEHNQLNLASHHFVVMDVFEYF
                     KYAKRKKLIFDVIVIDPPSFARNKKQTFSVSRDYHKLITEALDILSPKGTIIASTNAA
                     NMTVSQFKKQIIKGFGSRRPESMTLQQLPSDFTINKADERSNYLKVFTIKVRE"
     gene            3651..4337
                     /locus_tag="VC394_000871"
     CDS             3651..4337
                     /locus_tag="VC394_000871"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEP5994349.1"
                     /translation="MRIVAPVMPRHFDEAQAIDISKYEDVNLIEWRADFLPKDEIVAV
                     APAIFEKFAGKEIIFTLRTVQEGGNITLSSQEYVDIIKEINAIYNPDYIDFEYFTHKS
                     VFQEMLDFPNLILSYHNFEETPENLMEAFSEMTKLAPRVVKIAVMPQSEQDVLDLMNY
                     TRGFKTLNPEQEFATISMGKLGRLSRFAGDVIGSSWTYVSLDHVSGPGQVTLNDMKRI
                     IEVLEMDISN"
     gene            4431..5597
                     /gene="aroC"
                     /locus_tag="VC394_000872"
     CDS             4431..5597
                     /gene="aroC"
                     /locus_tag="VC394_000872"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEP5994350.1"
                     /translation="MRYLTAGESHGPSLTAIIEGIPAGLTLHPADIDHELQRRQGGYG
                     RGARMSIETDRVQISSGVRHGKTTGAPITLTVINKDHQKWLDVMAVGDIEETLKLKRR
                     VKHPRPGHADLVGGIKYHFNDLRDALERSSARETTMRVAVGAVAKRILAELGIDMLHH
                     ILIFGGITITIPSKLSFRELQERALHSELSIVNPKQEEEIKTYIDKIKKEGDTIGGII
                     ETIVQGVPAGLGSYVQWDKKLDAKLAQAVLSINAFKGVEFGVGFDMGFQKGSQVMDEI
                     TWTPTQGYGRQTNHLGGFEGGMTTGQPLVVKGVMKPIPTLYKPLMSVDIDSHEPYKAT
                     VERSDPTALPAAGVIMENVVATVLAKEILETFSSTTMSELQKAFSDYRAYVKQF"
     gene            5658..5999
                     /locus_tag="VC394_000873"
     CDS             5658..5999
                     /locus_tag="VC394_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEP5994351.1"
                     /translation="MSQEIYDYANQLERAVRALPEYQKVLEVKEAIQADASASELFDE
                     FVAMQEKIQGMMQSGQMPTAEEQTSIQELSQKIEANDQLKAYFEAQQALSVYMSDIER
                     IVFAPLKDLVK"
     gene            6221..7573
                     /gene="gorA"
                     /locus_tag="VC394_000874"
     CDS             6221..7573
                     /gene="gorA"
                     /locus_tag="VC394_000874"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEP5994352.1"
                     /translation="MVIPYDYIVIGGGSAGIASANRAAMHGAKVLLAEGKEIGGTCVN
                     LGCVPKKVMWYGAQVADILGTYAKDYGFDFKDKAFDFKQLKANRQAYIDRIHASYERG
                     FEQNGVDRIYDYAVFKDAHTVEIAGQLYTAPHILIATGGHPVFPDIEGAQYGISSDGF
                     FALDEVPKRTAVVGAGYIAVELAGVLHALGSKTDLFIRHDRPLRSFDKAIVDVLVEEM
                     AVGGPCLHPHSEVAKVVKNTDESLTLYLKDGQEVEVDQLIWAIGRKPNLEGFGLDKTG
                     VTLNDKGYIETDAYENTSVKGIYAVGDVNGKLALTPVAVAAGRRLSERLFNGKTDEKL
                     DYQNVATVIFSHPVIGSVGLSEEAAVKQYGQEAVKTYQSRFTSMFTAITNHRQPCLMK
                     LVTVGDTEKIVGLHGIGYGVDEMIQGFAVAIKMGATKADFDNTVAIHPTGSEEFVTMR
                     "
     gene            complement(7661..8929)
                     /locus_tag="VC394_000875"
     CDS             complement(7661..8929)
                     /locus_tag="VC394_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEP5994353.1"
                     /translation="MTTYDSISWIHTFKANGRKTDLARMAWLLEALGRPQDKFPAIHV
                     VGTNGKGSVTAYLQHILSTSGYQVGTFTSPFIISFHDRICLNGQPISDLELAQCVSVI
                     RPILTKMSLETNWDRPTEFELVTLIMFCYFANLKPVDIAIIEAGIGGKTDATNVFHAL
                     AVVCPSISFDHQERLGYSLAEIAHQKAEVIKAKEPVIIGQLEQEASQVFQQVTQKAGS
                     RLYQLGKDFLLNPSEKTFSFQHDNLNLTKLRLKLLGQHQKANACLAIMTAQLLRKSFP
                     KISPKTIQKGIEATTWPGRSEFIQPNLLLDGAHNPDSIAKLKSLLQEEFPKRPIHILF
                     AGLKRKPLADLLAQLDTFEVSVTTFDFPEANLLEDYPDKYPQVKDYRDWLQGSVTSDE
                     LFVVTGSLYFISEVRQYCLSNANIFKNIFP"
     gene            complement(8959..9399)
                     /locus_tag="VC394_000876"
     CDS             complement(8959..9399)
                     /locus_tag="VC394_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994354.1"
                     /translation="MSSRYSRQQKPRKSGGVPTEHIKTGFTAFQKSIALIGSILSIIV
                     ATITITRALQPVPDKTSNDTPKESSNTIVKIIEKESSQGNQNHTGTSETTDDNKTTLP
                     SSSTTPSHNETTPTAPETPSATEGTQLPNATGAEDANTGLPTNS"
     gene            9634..10776
                     /locus_tag="VC394_000877"
     CDS             9634..10776
                     /locus_tag="VC394_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEP5994355.1"
                     /translation="MIYFDNAATTIPYGEALKTYQEVATKIYGNPSSLHQLGTNASRI
                     LEASRKQIAGLLGVKSEEIFFTSGGTESANWAIKGIAFEKTAFGKHIIISAIEHPAVS
                     ESVKWLLTQGFEVSYAPVTTQGVVDVNALAELIRPDTILISIMAVNNEMGAIQPIRAI
                     SNLLANQPTITFHVDAVQAIGKIPLCDYMTNRVDLASFSGHKFHSVRGVGFLYKKAGK
                     RLNPLLSGGGQEQELRSTTENVAGIASMAKALRIVTEKQVSVLPKLTAMRDVIYKALS
                     AYPDVTVFSAQEGFAPNILTFGIRGVRGEVIVHAFEKYEIYISTTSACSSKAGKPAGS
                     LVAMGIPVKTAQTAVRISLDDDNDMGQVEQFLTIFKQIYDKTQKVR"
     gene            10788..12002
                     /gene="thiI"
                     /locus_tag="VC394_000878"
     CDS             10788..12002
                     /gene="thiI"
                     /locus_tag="VC394_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEP5994356.1"
                     /translation="MDYSEIMVRHGELSTKGKNRMRFINKLKNNIQDVLAPFPAIAVR
                     SDRDRTHVSLNGTDYQPIVEALKLVFGVQALSPVYKLEKSVPLLVTAVQDIMTSLYRD
                     GLTFKIATKRSDHAFELDSRELNSLLGGAVFEVLPNIQAQMKHPDVTLKVEIRDEAAY
                     ISYEEIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGLDIEVVHFASPPYTSPG
                     ALAKAQDLTRRLTRFGGNIQFIEVPFTEIQEEIKNKAPEAYLMTLTRRFMMRITDAIR
                     EQRKGLVIVNGESLGQVASQTLESMQAINAVTSTPIIRPVVTMDKLEIIEMAQAIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKLGNAEKYEERFDIDGLVQRAVSGIVVTEITPEIV
                     NDEVENLIDALL"
     gene            12040..13332
                     /locus_tag="VC394_000879"
     CDS             12040..13332
                     /locus_tag="VC394_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEP5994357.1"
                     /translation="MILLKSLYKGFFAAMKEENFEARCYNREKGGCVLIKTMQYKKTM
                     ATVVALIVALLLFGLIYDLLGVQKNKLAAQKSAQPKVKTARVVANGDILIHDILYMSA
                     RKADDTYDFTPYFEYVKDWISGADLAIGDYEGTISPDYPLAGYPLFNAPEEIAGALKN
                     TGYDVVDLAHNHILDSQLDGALNTKKVFHQLGIDSIGIYDKDRSKESFLIKNVNGIKI
                     AILGYSYGYNGMEATLSQEDYEKHMSDLDEAKIKKELQLAEKKADVTIVMPQMGTEYA
                     LEPTAEQKELYHKMIDWGADVVLGGHPHVIEPSETVIKGRQKKFIIYSMGNFISNQRL
                     ETVDDIWTERGLLMDLTFEKKDNKTKIKTVEAHPTMVLAKGKGIVGKEGFELYNYRTM
                     VLEDFIKGGKYHDKIDEETKAKVALAYQEINDLVNLKW"
     misc_feature    13437..13511
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            13546..13860
                     /gene="rplU"
                     /locus_tag="VC394_000880"
     CDS             13546..13860
                     /gene="rplU"
                     /locus_tag="VC394_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEP5994358.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAIYVEKIDAEAGAEVTFNEVVLVG
                     GDKTVVGTPVVEGATVVGTVEKQGKQKKVVTFKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     gene            13872..14198
                     /locus_tag="VC394_000881"
     CDS             13872..14198
                     /locus_tag="VC394_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEP5994359.1"
                     /translation="MIKAIFTRQKNGQLSSVTLTGHAGSGKHGFDIVCASVSTLAINF
                     VNSLEVLADCQALVDLNDVEGGYMAITIPPHDNKEEVQLLFESFLLGMTSLAKDSSKF
                     VNTQVI"
     gene            14226..14519
                     /gene="rpmA"
                     /locus_tag="VC394_000882"
     CDS             14226..14519
                     /gene="rpmA"
                     /locus_tag="VC394_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEP5994360.1"
                     /translation="MLKMNLANLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVSG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPVAK"
     gene            14877..15791
                     /locus_tag="VC394_000883"
     CDS             14877..15791
                     /locus_tag="VC394_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEP5994361.1"
                     /translation="MNIQQLRYVVAIANNGTFREAASKLFVSQPSLSVSIKDLEAELG
                     FQIFNRTTSGTVLTSQGLVFYEKALEVVKSFDSFEKTFSQADLDQNEFSIASQHYDFL
                     PPLITAFSQQYDGHRVFRIFESTTIQILDEVAQGNSEIGIIYLNVDNQKGLFQRMDKL
                     GLEYVSLIPFTTHIYLSKTHPLANREALYLSDIQGLPAVRFTQERDEYLYYSENFVDT
                     SECPRIYNVSDRATLNGILERTNAFATGSGFLDHRSVNGIKVIPLADHIDNQMIYVKR
                     KDKNLSVAGATFVTILKDYFDKRRKALT"
     gene            15788..16246
                     /locus_tag="VC394_000884"
     CDS             15788..16246
                     /locus_tag="VC394_000884"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEP5994362.1"
                     /translation="MKKRLFVLSLILLVALDQLSKFWIVSHIALGEVKPFIPGIVSLT
                     YLQNNGAAFSILQDQQWFFVVITVLVIGYAIYYLATHPHLNIWKQLALLLIISGGIGN
                     FIDRLRLAYVIDMVHLDFVDFAIFNVADSYLTVGVILLVICLWKEEDYGN"
     gene            16236..17126
                     /locus_tag="VC394_000885"
     CDS             16236..17126
                     /locus_tag="VC394_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEP5994363.1"
                     /translation="MEINVITSGQRLDKALADLSPLSRGQANDQIKQGLVLVNGQQKK
                     AKYTVQAGDVICFELPKEEVLEYQAQNIPLDIIYEDDTLAIINKPQGMVVHPSAGHPS
                     GTMVNALMYHIKDLSSINGVVRPGIVHRIDKDTSGLLMVAKTDAAHQALAEELKAKKS
                     LRKYLAIVHGNLPNDRGMIEAPIGRSEKDRKKQAVTAKGKEAVTRFTVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHPVAGDPLYGPRKTLSGHGQFLHAKTLGLTHPMTGKEM
                     IFTVEAPEIFQKVLKLLRKK"
     gene            17521..18042
                     /gene="pyrR"
                     /locus_tag="VC394_000886"
     CDS             17521..18042
                     /gene="pyrR"
                     /locus_tag="VC394_000886"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEP5994364.1"
                     /translation="MKTKEIVDDVTMKRAITRITYEIIERNKQLDNVVLAGIKTRGVF
                     LARRIQERLHQLEGLDLPIGELDIKPFRDDMRVEEDTTLMSVDITGKDVILIDDVLYT
                     GRTIRAAIDNLVSLGRPARVSLAVLVDRGHRELPIRADYVGKNIPTSSVEEIVVEVVE
                     VDGRDRVSIIDPT"
     gene            18058..19317
                     /locus_tag="VC394_000887"
     CDS             18058..19317
                     /locus_tag="VC394_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEP5994365.1"
                     /translation="MKDVIYDVEEVPKAGMLVGLSFQHLFAMFGATVLVPILVGIDPS
                     VALLSSGLGTLAHLSVTKFKIPAYMGSSFAYIAAMQLLMKTNGIGAVAQGAMTGGLVY
                     LIVALIVKVIGNDWIDKILPPIVVGPIVMVIGLSLASTAVNDVMLKNGNYNLTYLVIG
                     LVTLLSVIFFNIYGKGIVAIVPLLLGLLVGYVVALLVGVLTGQEIVDFTNVAQAKWFS
                     IPSVEIPFLTYGVKFYPSAILTMAPIAFVTMTEHFGHIMVLNSLTKRDYFKDPGLEKT
                     LTGDGFAQIIAGFLGAPPVTSYGENIGVMALNKIFSVYVIAGAAVIAALLSFIGKVSA
                     LIQSIPTPVIGGISVALFGVIASSGLKILIESKVDMDNKKNLLIASVILVSGIGGLML
                     QVNGLQISGVAFSTLLGIILYQVLPEK"
     gene            19378..20313
                     /locus_tag="VC394_000888"
     CDS             19378..20313
                     /locus_tag="VC394_000888"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEP5994366.1"
                     /translation="MSVVNNRVALTNLVSMEALTTEEVLGLINRGSEYKAGKVVISDH
                     QKDLVANLFFENSTRTHKSFEVAEKKLGLTVLDFNADASAVNKGESLYDTVLTMSALG
                     TDICVIRHPEDDYYKELVESPTITASIVNGGDGSGQHPSQCLLDLLTIYEEFGRFEGL
                     KIAIAGDLTHSRVAKSNMQILKRLGAELYFYGPEEWYSEAFNAYGTYIAIDQIIKELD
                     VLMLLRVQHERHDGHQSFSKEGYHQAFGLTQERYQQLKDSAIIMHPAPVNRDVEIADS
                     LVEAPKARIVSQMANGVFVRMAIIEAILNGRNKNS"
     gene            20357..21439
                     /gene="carA"
                     /locus_tag="VC394_000889"
     CDS             20357..21439
                     /gene="carA"
                     /locus_tag="VC394_000889"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEP5994367.1"
                     /translation="MTKRLLILEDGTIFEGEPFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLIGNYGINRDDYESISPTCKGVVVSEVSRLASNWRKQMTLDAFLK
                     IKGIPGISGIDTRALTKIIRQHGTMKATMADDGDSIQHLKDQLRATVLPTNTIEQVST
                     KTAYPAPGIGKNIVLVDFGLKHSILREFSKRQCNITVVPFNITAEEVLQLNPDGLMLS
                     NGPGNPEDLPEALDMIRGVQGKIPIFGICMGHQLFSLANGAKTCKMTFGHRGFNHAVR
                     EIATGRIDFTSQNHGYAVERSSLPDTLMVTHEDINDKTVEGVKHRDFPAFSVQFHPDA
                     APGPHDASYLFDEFLEMIDSWRCTSK"
     gene            21685..24861
                     /gene="carB"
                     /locus_tag="VC394_000890"
     CDS             21685..24861
                     /gene="carB"
                     /locus_tag="VC394_000890"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004218948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEP5994368.1"
                     /translation="MPKRKDIQKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYKV
                     ILVNSNPATIMTDKEIADKVYIEPLTLEFVNRIIRKERPDAILPTLGGQTGLNMAMAL
                     SKAGILDDLEIELLGTKLSAIDQAEDRDLFKQLMQELDQPIPESTIVKTVDEAVTFAR
                     DIGYPVIVRPAFTLGGTGGGICSSEEELCEITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPTQTLSDIENQMLRDASLKIIRALK
                     IEGGCNVQLALDPYSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEM
                     INPITGTTYAMFEPTLDYVVAKIPRFPFDKFEHGERQLGTQMKATGEVMAIGRNLEES
                     LLKACRSLEIGVCHNEMTSLSNISDEELVTKVIKAQDDRLFYLSEAIRRGYSIEELES
                     LTKIDLFFLDKLLHIVEIEQDLQMHVEHLESLKKAKRYGFSDQKIAEIWQKDESDIRA
                     MRHSHSLYPVYKMVDTCAAEFDAKTPYFYSTYELENESVQSNKESILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQKAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAQALSEAGVTILGTQVEDLDRAEDRDLFEKALKELGIPQP
                     QGQTATNEEEALEAAKKIGFPVLVRPSYVLGGRAMEIVENKEDLREYIRTAVKASPEH
                     PILVDSYIFGKECEVDAISDGKSVLIPGIMEHIERAGVHSGDSMAVYPPQQLSKQIQE
                     TIAEYTKRLAIGLNCIGMMNVQFVIKNEQVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKLILGQTLKDLGYEDGLYPQSQLVHIKAPVFSFTKLAQVDSLLGPEMKSTGEVMGSD
                     TSLEKALYKAFEANNSHLSEFGQIVFTIADDSKAEALSLARRFKAIGYQIMATQGTAA
                     YFAEQGLSACLVGKIGDAANDIPTLVRHGHVQAIVNTVGIKRTADKDGQMIRSSAIEQ
                     GVPLFTALDTAKAMLTVLESRCFNIEAI"
     gene            25068..26339
                     /locus_tag="VC394_000891"
     CDS             25068..26339
                     /locus_tag="VC394_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEP5994369.1"
                     /translation="MSKRGKIKITTKTKLITASVITLVLIITGVVLWKQQQNTLTADI
                     AKEPYSTVSVTEGSIASSTLLSGTVKALSEEYIYFDANKGNDATVTVKVGDQVTQGQQ
                     LVQYNTTTAQSAYDTAVRSLNKIGRQINHLKTYGVPAVSTETNKDEATGEETTTTVQP
                     SAQQNANYKQQLQDLNDAYADAQAEVNKAQIALNDTVVISSVSGTVVEVNNDIDPSSK
                     NSQTLVHVATEGQLQVKGTLTEYDLANVKVGQSVKIKSKVYSNQEWTGKISYVSNYPT
                     ESNAGSTTPAGSTGAGSSTGAAYDYKIDIISPLNQLKQGFTVSVEVVNEAKQALVPLT
                     AVIKKDKKHYVWTYDDATGKAKKVEVTLGNADAQQQEIHKGVAVGDIVIANPDKNIKP
                     DKKLEGVISIGTNTKPEKDSQSKNKKSGVDK"
     gene            26339..27049
                     /locus_tag="VC394_000892"
     CDS             26339..27049
                     /locus_tag="VC394_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5994370.1"
                     /translation="MSHEKKQLMQLSNIVKSYQNGDQVLKVLKGINLTVYEGEFLAIM
                     GPSGSGKSTLMNIIGLLDRPTSGDYTLHNTKIEILNDRELAKVRNDEIGFVFQQFFLL
                     AKLTALQNVELPLIYAGVNVSKRREQAKQFLEKVGLGRRIKHLPSELSGGQKQRVAIA
                     RALVNDPSIILADEPTGALDTKTGQQIMELLTELNKEGKTIIMVTHEPEIADFATRKI
                     IIRDGDITTDTTASVVID"
     gene            27061..28281
                     /locus_tag="VC394_000893"
     CDS             27061..28281
                     /locus_tag="VC394_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP5994371.1"
                     /translation="MENWKFALSSIWGHKMRSILTMLGIIIGVAAVVIIMGLGNAMKN
                     SVTSTFSSKQKDIQLYFQEKGEEEDLYAGLHTHENNHEVKPEWLEQIVKDIDGIDSYY
                     FTNSATSTISYEKKKVDNASIIGVSKDYFNIKNYDIVAGRTLTDNDYSNFSRIILLDT
                     VLADDLFGEGNYKSALNKVVSLSDKDYLVIGVYKTDQTPVSFDGLSGGAVMANTQVAS
                     EFGTKEIGSIYIHVNDIQNSMNLGNQAADMLTNISHIKDGQYAVPDNSKIVEEINSQF
                     SIMTTVIGSIAAISLLVGGIGVMNIMLVSVTERTREIGLRKALGATRLKILSQFLIES
                     VVLTVLGGLIGLLLAQLSVGALGNAMTLKGACISLDVALIAVLFSASIGVFFGMLPAN
                     KASKLDPIEALRYE"
     gene            28535..>30234
                     /locus_tag="VC394_000894"
     CDS             28535..>30234
                     /locus_tag="VC394_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HEP5994372.1"
                     /translation="MTFLSDLISLMTKIRLSWVIKAGIFQLLFVTIANIVLSEFFYFI
                     LDVTGQYHLDKDNVVTFLKNPIALALLGAYLFLLAAFIHLEFFALYRIIADQEISFYL
                     FRKQFSYYLRGLWKTFSGYQLLLFLLYILLTIPVLHIGLSSVITQKLYLPEFIVGELS
                     KITSTKYLLYGSLILVFYLNLRLVYFLPLIAINHRTVAQAWRESWQKTKKKHVLLWMK
                     LFAINGLTLVVVSLAISMILIFVDMFNPKGNNIIVQLGALTFTWELIFFTTIFFKLCS
                     AMILKEAIEPQKQYDEPRRSNKAYVVIFIVVTVGFAYQSLERLTFFDTSHSKTVIAHR
                     GLVSAGVENSLEALEGAKKAGSDYVELDLILTKDNHFVVSHDNRLKRLAGVNKTIRNL
                     TLKEVEHLTSHQGHFSGRFVSFDTFYQKAKKLNMPLLIELKPIGTEPGNYVDLFLETY
                     HRLGISKDNKVMSLDLEVIEAIKKKNPSITTGYIIPIQFGFFGDEFVDFYVIEDFSYR
                     SYLSSQAFWNNKEIYVWTINDPKRIEHYLLKPIQGIITDQPALTNQLIKDLKQDNSYF
                     "
BASE COUNT         9512 a         5024 c         6342 g         9356 t
ORIGIN      
        1 cccatgtttt attaccagta atgtgggata tttagatggt agcaaggagt tttatagctg
       61 atttgttttc tttaggtcta attagcatat tttgattact gataaacttg aatatcgcca
      121 ataaaaaaga agcaaaatta ttattttgct tctagtcaga tcattaaatt gattattctt
      181 tatttttggg gttatcctct tttagttcag aagttgccgc gtcagcttct acgggattta
      241 gttctaagta agatggtgct ttaaaaagtt tctgagttga tttatgacca tttttactgt
      301 ataaacttgt tgactctgat ccgagttttg ctgaaatatc tttcagttgt tttaactgct
      361 tagtgtagat aaactggtta ggatcaatag cttttagacc attttgagta tcaaagcgca
      421 ggagatcacc cgtttgaata gcgtcgctag ctgctagttg ttgagcaaca gcagagcgaa
      481 tttccttagt tttggctata gtcatttcat cgggattagt gatttctaaa cctgtttgcg
      541 tgttgtacag acgtccacta tagttagtgt attcaggagt catataagtc ccagatgttc
      601 gttgtgccac aatctggcta ttttgaggtg ataataagtc ttgtcctaat tgaacaaact
      661 gactagtgtc aataccaagt atgtggagta aagtaggaag agcatcgatt tcaccgccaa
      721 aggtttcttt gatactacca ttcgtataac ctggaatatg gatcatatac gggactcgtt
      781 gtagcatagc attatcatat tcagaccaag tttcagaatc tttaccaaga agtggagcaa
      841 gactagaatt acgagaattt gaaataccat aatgatcacc atataaaaca aaaatagaat
      901 tctcgtacaa accagtggct ttcaagtaat caaaaaaaga tttaagggca gcgtctaaat
      961 aattggcagt agcaaagtaa ccattgatcg tttcatcgtc tgttttggct aaaggaaaac
     1021 cttcttcact actttcgcct tttagactag tataaggata gtgattactc acagtgataa
     1081 acttggtata aaaaggttgt tgcatctgtt caagatattt tatggaatcc ttaaacatgt
     1141 acttatcatt taacccatat tgaaaagagt tcttactatt ttgtttagag aagtagctag
     1201 aatcaaaaaa ataattatag ccccattgtt tataagcatt attgcgattc cagaaagttc
     1261 caacgttacc atgaaagaca gcgctggtat agccaccttt ttgggttaaa atacttggag
     1321 tagcaaattg tgtattttca ccaccgtagt tcaccataaa agaaccacta tttaaaccaa
     1381 ataaggaatt ctccatcatg gtttcagcat cagaagtttt acctgcttta acctgatgaa
     1441 aaaagttagg gaatgctaag gtagcatttg agtgataaag cgagttgata aaaggtgtta
     1501 cctcatattc tttatctcct tcttttagtt tataatctat taagaattgt tgaaagcttt
     1561 ctaaatgaag aacaataata tttttgcctt ttccaattcc aaaatactga ggatcaggtg
     1621 ctgcataatg tcctttgaca taagttttaa catcaattaa ttcttctgca gtagctccat
     1681 ttcgttcttt ttgagcttga taggtttgat tgccgctata gagggtaaac gctggtagtc
     1741 ctaaagctct cacaatataa gtatttgaaa agccacgtgt taatagttcc ggacgatcaa
     1801 tttctgctaa aaaaagatta atagaaagca atagactaga taaagcggtg atggcaaatg
     1861 ctgcgcgctt gttaaaagga cgttggtcat ttttgatttt tttagtgata gataaactaa
     1921 ttagaataat aaaatcaaga atatagataa tatcccagat gcgtaaaagg tttaaagcag
     1981 agtcccctaa tccagcggag actttactac tagctagcat ggcactcact gtaataaagt
     2041 ctgaaaattc tcggtaatat atagaatttg atatcaagag aatgttaagg ataatataaa
     2101 ccacccaaga acagatataa aaggctcggg tattcttaac gtaaagagca acccccaaaa
     2161 gtaaaaaagc tagtgggatt ggattgataa tagttagaaa gacttggtat atatttccca
     2221 aatctagtga aaagtctgta tgatatgccc acagggtctt tatccagtaa caaaagagta
     2281 gagtaattat aaaacccaat cttgtgttga taaaacctgt gataagtgtt ttaaatttct
     2341 tcacagtagc aacttcctta tcgttttttc ctaaagatgt catctccaaa taaaatctca
     2401 gattaaatta taccaaaaat gtgaagctaa tgcttgttgt aagttcaaat ttagtaggat
     2461 tttttatcag attttgttat aataaaaact atgaataaac tctatattga ttcttttgtc
     2521 gaaaagaagc tgacagcagg ggtacaatta ttagatgaaa aagatttttc taatataaag
     2581 gaaaaaaatc agttggttca actggtgacc aagtccaatc gtcccattgg aacagcatat
     2641 atctctaagc aaaataaagg aattggttgg tatctaggac cagaaaaaat cgacttgtct
     2701 atctcttatt ttgttagtct attttcagtt gctaaggcca aaagacaaga ttttgctcaa
     2761 tcggacgaga ccaatgctta tcgccttttt aatcaagaag gagacggttt tggtggagta
     2821 acaattgatc tttataaaga ttttgcagtc ttttcatggt acaatgcctt tgtctatgac
     2881 aaaaaagaaa tgattatgga ggcttttcaa caggtttttc ctgaagtcaa aggagcttat
     2941 gaaaagtgtc gctttaaagg tccagatact gaaactgctc atctgtatgg tgagctggca
     3001 caagaaacct tttcgatttt agaaaatggc atagcctatc aagtcttctt gaatgagggc
     3061 ttaatgacag gtgtttttct agaccagcat gatgttagaa gagcactagt agatggttta
     3121 gctatgggca agtcccttct taacctattt tcctatacgg ctgccttttc tgtagcagct
     3181 gctatgggtg gtgctatcga gaccacttct gtggacttgg ctaaacgatc gagagaatta
     3241 tcactagctc attttgagca taaccagtta aacttagcct cacatcattt tgtggtgatg
     3301 gatgtttttg aatacttcaa atatgccaaa cggaaaaaat taatctttga tgtgattgtg
     3361 attgacccac caagctttgc tagaaataaa aaacaaactt tctctgtgag cagggactac
     3421 cacaaattaa tcacagaggc cttggacata ttgagtccta agggaactat tatagcttca
     3481 acaaatgctg ctaatatgac tgttagccag ttcaaaaagc aaattatcaa aggatttgga
     3541 agtcgtcgtc ctgagtctat gactctccag cagcttccga gtgactttac tatcaataaa
     3601 gcagatgaaa gaagtaacta tttaaaggta tttactataa aggtacgaga atgagaattg
     3661 tagcaccagt catgccaaga cattttgacg aggctcaagc cattgatatt tctaaatatg
     3721 aagatgttaa tttaattgaa tggcgagcag actttcttcc taaggatgag attgtcgcag
     3781 tagcaccagc tatttttgaa aaatttgccg gaaaagaaat tatctttacc cttcggaccg
     3841 ttcaagaagg ggggaatatt accctttcaa gtcaggagta tgttgatatt atcaaagaaa
     3901 tcaatgctat ttataatcca gactatattg actttgagta ttttacgcac aagtcagtat
     3961 ttcaagaaat gcttgatttt ccaaatctta ttttgtctta tcacaacttt gaagagactc
     4021 ctgaaaattt aatggaagct ttttcagaaa tgaccaagtt ggcaccgcgt gtggttaaaa
     4081 ttgctgtgat gccccagagt gaacaagatg ttctagattt gatgaactat accagaggct
     4141 ttaagacgct caatcctgag caagaatttg caactatatc tatggggaaa ttaggaagat
     4201 tgtctcgttt tgcgggagat gtcattggct cttcctggac atacgtttca ttagatcatg
     4261 ttagcggtcc aggtcaagtg actctcaatg atatgaaacg tattattgag gtcttagaaa
     4321 tggatatctc taactagtga atggtctaaa aatattattt ctacaatagt taagaaatga
     4381 tgtttttaga ttaatgtttt taaaataagt ggaaccaaaa aggagaaaaa ttgcgttatt
     4441 taacagcagg agaatctcat gggccatcct taacagctat tattgaaggt ataccagcag
     4501 gattaactct tcatccagcg gatattgatc acgaattaca gcgacgtcaa ggaggttatg
     4561 gccgtggtgc cagaatgtct attgagactg atcgggttca aatatcctca ggagttcgtc
     4621 atggcaaaac aacaggggcc cccattacct taacggtaat taataaggac caccaaaaat
     4681 ggctagatgt catggctgta ggagacattg aagagactct caagttaaag cgtcgtgtta
     4741 agcatcctag accaggacat gcggatttgg taggaggtat taaatatcat tttaacgatt
     4801 taagagatgc tttagagcgt tcctcggcta gagaaacaac gatgagagtg gctgtaggcg
     4861 ctgttgctaa acgtatctta gctgaacttg gaattgatat gttacatcat atcttaatat
     4921 ttggtggtat aacgatcaca ataccttcta agctctcttt tagagagttg caagaaaggg
     4981 ctctccattc agagttatct attgttaatc caaagcaaga agaagaaatt aaaacgtata
     5041 ttgataagat aaaaaaagaa ggggatacta ttggtggtat tatcgaaacg atagttcaag
     5101 gggtgccagc aggtcttgga tcttatgtcc aatgggataa aaaattagat gccaagctgg
     5161 ctcaagcagt tctttccatt aatgctttta aaggggtgga atttggagtt gggtttgata
     5221 tgggattcca aaagggctct caggtaatgg atgaaatcac ctggacacct acccaaggct
     5281 atggtcgtca aacaaaccac ttaggtggct ttgaaggtgg catgaccaca ggacaacctc
     5341 ttgttgttaa aggggttatg aagcccattc ctactttgta taaaccactc atgtcggtag
     5401 atattgatag tcatgaacct tataaagcaa cggtggaacg ctctgatccg acggccttac
     5461 cagctgcagg ggttatcatg gaaaatgtgg tggcgacggt gcttgccaaa gagattttag
     5521 agactttttc ttctactacc atgtctgaat tgcaaaaagc tttttcggac tatcgtgcct
     5581 acgtcaagca attttagata gcgagatact ggcttttatg gtatcataca ggtattaatg
     5641 aacaaaagga gactgtcatg tcacaagaaa tttatgacta tgctaatcaa cttgaaagag
     5701 cagttcgtgc tctgccagaa taccaaaaag tgttagaagt aaaagaggcc attcaagcag
     5761 acgctagtgc tagcgaactg tttgatgagt ttgtcgctat gcaagaaaaa atccaaggga
     5821 tgatgcaatc aggccaaatg ccaacggctg aagaacaaac aagcattcag gaacttagcc
     5881 aaaagattga agctaatgac cagctaaaag cttattttga agcgcagcag gccttatctg
     5941 tttacatgag tgatattgag cgtattgtat ttgctccgtt gaaagattta gtcaagtaag
     6001 ccaattaaga taggaaaaaa aacagattgc tggcagctcg tcaggatctg ttttttagtg
     6061 tttattttct ttgtatcaga aagttgagcc tctttcttat ttgtgatata gtcaaataaa
     6121 ttgaccaaaa tagcaggcca gtagtggctt taatgaaggc aagttgtctg ctaactggct
     6181 atgctaaaac atcagtatta gaaagtaaaa ggttataaaa atggttattc cttatgatta
     6241 tattgttatt ggtggaggga gtgcaggtat tgcttctgcg aacagggcag ccatgcatgg
     6301 ggccaaagta ttgttagctg aaggtaaaga gattggggga acctgtgtta acctaggctg
     6361 cgttcctaaa aaagtcatgt ggtatggcgc tcaagtagca gatattttag ggacttacgc
     6421 taaggattat ggatttgact ttaaagacaa agcatttgat tttaaacaat tgaaagcaaa
     6481 tcgtcaagct tatattgatc ggattcatgc ttcttatgag cgaggctttg agcagaatgg
     6541 tgttgaccgt atttatgatt atgctgtttt caaagatgct catacggtag aaattgcagg
     6601 gcagctttat accgcaccgc atattttgat tgccacagga ggacatcctg tttttccaga
     6661 tattgagggg gcacaatacg gtatcagctc ggacggcttt tttgccttag atgaggtacc
     6721 aaaacgtacg gctgtggttg gggcaggcta tattgctgtg gaacttgccg gtgttttgca
     6781 tgctttgggg tcgaagacgg atttgtttat ccgtcacgat cgacctttaa gaagttttga
     6841 caaagccatt gtggatgttt tagttgagga aatggcagtt ggtggtcctt gcttacaccc
     6901 tcattcagaa gtggcaaagg ttgtcaaaaa tactgatgag tccttgaccc tttacctcaa
     6961 agatggccaa gaagttgagg ttgaccagct catttgggct atcggtcgca agcctaatct
     7021 agaaggtttt ggtcttgaca aaactggagt gacgttaaat gataaaggtt atattgagac
     7081 agatgcctat gaaaacacgt ctgtcaaagg tatttatgcc gtgggagatg ttaatgggaa
     7141 actggcttta actccagttg ctgtggcagc tggacgtcgc ctatcagagc gtcttttcaa
     7201 tggcaaaaca gacgagaaat tggactacca aaacgttgct actgtgattt ttagtcaccc
     7261 tgttattggc tctgttggct tgtcagaaga agcagcagtc aaacagtatg ggcaagaagc
     7321 agttaaaaca tatcaatcac gttttacgtc tatgtttact gctatcacta accatcgcca
     7381 accttgtctg atgaagttgg taacagtcgg agataccgaa aaaatagttg gtcttcacgg
     7441 aatcggctat ggggttgatg aaatgattca gggctttgca gtagccatca aaatgggagc
     7501 aaccaaggcg gattttgata acaccgttgc tattcaccca actggctcag aagaatttgt
     7561 caccatgcgc taaaaaatgt agcctaaggc aataaaaaat gatgttaaca gtttttgagg
     7621 aaagtcttct aaactgctag catcgttttt tgttaggtga ttaggggaat atgtttttaa
     7681 agatatttgc gttgctcaga caatattgcc ttacctcgga gataaaataa agtgaccctg
     7741 taacaacaaa gagttcatca gatgttacgg aaccttgtaa ccagtctctg taatctttta
     7801 cctgtgggta tttatcagga taatcctcta aaagatttgc ttcagggaaa tcaaaagtcg
     7861 tgacagatac ttcaaaagta tctagttgag ccagcaagtc ggctaaaggc ttacgtttga
     7921 gacctgcaaa gagaatatga ataggacgtt tgggaaattc ttcttgcagt agagacttta
     7981 atttagcaat ggaatctgga ttgtgagcgc cgtctaatag aagattaggt tgaataaatt
     8041 cggaccggcc aggccaagtt gttgcctcaa ttcctttttg aatcgttttt ggagatattt
     8101 taggaaagga ttttctaagt aattgagctg tcataatggc taaacaggca ttcgcttttt
     8161 gatgttgacc taatagtttg agacggagtt tggttaaatt caagttatcg tgttgaaagg
     8221 aaaacgtctt ttctgaagga ttgagcaaaa aatcttttcc taattgatat aagcgtgaac
     8281 cggctttttg ggtaacttgt tgaaagactt gactagcctc ctgctcaagt tgaccgataa
     8341 taacgggttc tttagctttg ataacttctg ctttttggtg agcaatttct gctaggctat
     8401 aacctaaacg ttcttggtga tcaaaactaa tagaagggca tacgacggcc agagcatgaa
     8461 aaacattggt tgcgtcggtt ttcccaccaa tccctgcctc aataatggca atatcaacag
     8521 gtttaagatt ggcaaagtag caaaacatta tgagagtcac taactcaaac tcggtaggtc
     8581 tatcccaatt tgtttcaagg gacattttag tgagaatggg tctaatcacg ctcacacatt
     8641 gcgccaattc taggtctgaa atgggctggc cattaaggca aatccgatcg tgaaaagaga
     8701 taataaaagg agatgtaaag gttccgacct gataaccaga agttgacagg atatgctgta
     8761 agtaggctgt cacagagccc ttgccgttag ttccaacaac gtgaatagca gggaatttgt
     8821 cttgtggccg gcctaatgcc tcaagtaacc aagccattct ggccaaatcg gtcttgcgac
     8881 cattagcctt aaaggtatga atccaggaaa tgctatcata ggtagtcatt actatttctt
     8941 gtccttctaa caatgctttt aagagttcgt agggagacca gtattagcat cctcagctcc
     9001 agttgcatta ggaagttgcg tcccttcagt ggctgatggt gtctcaggag ctgtaggagt
     9061 tgtctcgttg tgagaaggtg tggtagaaga tgacggaaga gttgtcttat tatcatctgt
     9121 ggtttctgaa gtacctgtat ggttttgatt accctgagaa gactcttttt caataatctt
     9181 gacaatggta ttactcgatt ctttgggtgt atcattagag gtcttatctg gtacaggctg
     9241 cagtgctctt gtaatggtaa tggttgccac aatgatactc aaaatactac caataagtgc
     9301 aatggatttt tggaaagctg taaaacctgt tttaatatgc tcggtaggga cacctcctga
     9361 tttacgaggt ttttgttgac gggaataacg tgatgacata tttttctcct ttaaaatcat
     9421 cgtaatgcca agattaagga ggaggaaata gaagtcctag ctcctcttta acacttcatc
     9481 tatcagtata atctagaaaa aaagtgaagt caacggttta ccttatgtct aggacaagga
     9541 agtcctttgg gcttattgta atacgaatat tcttgatttc attttagcta ttttgtgata
     9601 gaataaagta ttgagtaaaa atgaaggtga cacatgattt attttgataa tgcagccaca
     9661 accatacctt atggagaggc tcttaaaact taccaagaag tagcaacaaa aatatatggc
     9721 aatccctcaa gtctgcacca attagggaca aatgcgtctc gcattttaga agcttcccgt
     9781 aaacagattg ctggtttgtt gggtgttaag tcagaagaaa tcttttttac gtctggtggg
     9841 actgaaagtg ctaattgggc tattaaggga attgcttttg aaaaaactgc ttttggcaaa
     9901 catattatta tctcagccat tgaacaccct gccgtgagtg aaagtgtcaa atggcttctc
     9961 acacaaggat tcgaagtttc ttatgctcca gtgacaacac agggagttgt tgatgtgaat
    10021 gctttagctg agctgattag accagacacc atcttaatct cgattatggc tgttaataat
    10081 gaaatgggag ctatccaacc gattagagcc atttcaaacc tattggctaa ccaaccaacg
    10141 atcacctttc acgttgatgc tgttcaagcc attggtaaaa taccactctg tgattatatg
    10201 actaatcgtg tggatttggc ttctttttca gggcacaaat ttcatagtgt tcgtggtgtt
    10261 ggcttccttt ataaaaaagc aggcaagcga ctcaatcctc tcttgtcagg cggcggccaa
    10321 gagcaggagt tacgttcaac cactgaaaat gttgcaggaa ttgctagcat ggccaaagca
    10381 ttaagaatag tcactgaaaa acaagtgagc gtcttaccta aactaacagc gatgcgagat
    10441 gtgatttaca aagctttgtc cgcttaccca gatgtgacag tattttcagc tcaagaaggc
    10501 tttgcgccca atattttgac ctttgggatt agaggggttc gaggagaagt tattgtccac
    10561 gcctttgaaa aatatgagat ttatatttcg accacaagtg catgttcgtc caaagcaggt
    10621 aagccagcag gaagcctagt ggctatgggg attcctgtaa aaactgccca gacagccgtt
    10681 cgtattagtc tagacgatga caacgatatg ggacaagtag agcagttttt gaccatattc
    10741 aaacagattt atgataaaac acagaaagtt aggtaagatt aggtaagatg gactattcag
    10801 aaattatggt tcgccatggt gaactgtcaa ctaaagggaa gaatcgcatg cgattcatta
    10861 acaaacttaa aaacaatatt caagacgtac tggctccttt cccagccatt gctgtccgtt
    10921 cagatcgtga taggacccat gtgtctctaa atggcacaga ttatcagcct attgtagaag
    10981 ctttaaagct ggtgtttggt gttcaagctc tatctcctgt ttataaactt gaaaaaagtg
    11041 tccctctttt ggtaacagct gttcaagata tcatgacttc tctttatcgt gatggactga
    11101 cgtttaagat tgccactaag cgtagtgatc acgcgtttga attggatagt cgtgagctta
    11161 acagtcttct tggtggagct gtttttgagg ttttaccaaa tatccaagcc cagatgaaac
    11221 atcccgatgt gactctcaaa gtcgaaattc gtgatgaagc agcttacatt tcttatgaag
    11281 agattaaggg agcaggcggg ttaccagtag gaacgtcagg gaaagggatg ctgatgttat
    11341 cgggcggtat tgattcacca gtagctgggt atttagccct taaacgtggc ctcgatattg
    11401 aagttgttca ttttgctagc cctccgtaca ccagtccagg tgccttggct aaagcccaag
    11461 accttactcg tcggttaact cgttttggtg gaaatatcca atttatcgag gttcctttta
    11521 cagaaattca agaagaaatt aaaaataagg caccagaggc ttaccttatg acattaaccc
    11581 gccgctttat gatgcgcatc acagatgcca ttcgtgagca acgtaagggt cttgttattg
    11641 ttaacggtga gagcctaggt caggttgcta gtcaaaccct tgaaagcatg caggccatta
    11701 atgcggtaac atcaacccca attattagac cagtcgtgac aatggataaa cttgaaatta
    11761 tcgagatggc tcaggcgatt gatacttttg acatttctat tcaaccgttc gaagattgct
    11821 gtactatttt tgcaccagac cgtcctaaaa ccaaccctaa actaggtaat gctgaaaaat
    11881 atgaagaacg ctttgatatt gatggtcttg tccagcgtgc tgtgtcagga attgttgtca
    11941 cagaaatcac acctgaaatc gtcaatgatg aggttgaaaa tctcattgat gctttacttt
    12001 aaaagaaaag atcaagtgta aatcttttag tagtcagtaa tgatactatt gaaaagcctc
    12061 tataagggct tttttgctgc aatgaaggag gaaaattttg aggcaagatg ttataataga
    12121 gaaaaaggag ggtgtgtctt gattaaaact atgcaataca aaaaaacgat ggcaacagtt
    12181 gttgctctta tcgtcgcact tttgctattt ggcttgattt atgatttact tggggttcaa
    12241 aaaaataagc tagcagctca aaaatctgct cagccaaagg tcaaaacagc gcgtgttgta
    12301 gctaatggcg acattttaat tcatgatatt ctctacatga gtgctagaaa agctgacgat
    12361 acctatgatt tcactcctta ttttgaatat gttaaggact ggattagcgg tgctgactta
    12421 gctattggag attatgaagg gaccattagc ccagattatc ccttagcagg ctatccctta
    12481 ttcaatgctc cagaagagat tgcaggtgct ctgaaaaata ctggctatga tgtagtcgat
    12541 ttagcccata atcatatctt agattcccaa ttagacggtg cccttaacac taaaaaggtt
    12601 tttcatcaat taggtataga cagtattggc atctatgaca aggatcgttc aaaagaatcc
    12661 ttcttaatta aaaatgtcaa tggtatcaaa attgcaattt taggttattc ttatggttat
    12721 aacggcatgg aggccacact tagccaagaa gactatgaga aacacatgtc tgatttagat
    12781 gaagctaaga taaaaaaaga acttcagcta gctgaaaaaa aggctgacgt gaccattgtc
    12841 atgccacaaa tgggaacaga atatgcctta gaaccgacag cagagcaaaa agaactttac
    12901 cacaaaatga ttgattgggg agctgacgtc gtcctaggag gccatccgca tgttattgaa
    12961 ccttcagaga cagttataaa aggtaggcaa aagaaattca ttatttactc tatgggaaat
    13021 tttatttcaa atcagcggct tgaaaccgta gatgatatct ggactgagcg tggcctattg
    13081 atggacctta cttttgagaa aaaagataac aaaacaaaaa ttaaaacagt tgaggcccat
    13141 ccaaccatgg ttttagccaa aggtaaaggc attgtgggta aagaaggttt tgaactatac
    13201 aattatcgaa caatggtttt agaagatttt atcaaaggtg gaaaatacca cgacaagatt
    13261 gatgaggaaa ccaaagcaaa agtagcactt gcttatcagg aaattaatga tttagttaac
    13321 ctgaaatggt aatgtttggt ggtttagcat ctaacgataa aaaatagaga ttaaacttaa
    13381 tttttccttg ataaaaagca gttatggtgc taaaatagta acgttgacta aatgcacatc
    13441 ctgtgcaacc gcgcgaaaat cgttgtcaag tagaccaagt ctcacgattc caggcgagtc
    13501 ttcacagagg gaaacctcaa acaaaaattt tataggaggt gcatcatgag cacatacgca
    13561 atcatcaaaa ctggtggaaa acaagttaaa gttgaagtag gtcaagcaat ctacgttgag
    13621 aaaattgacg ctgaagctgg cgcagaagtc acttttaacg aagttgttct tgtaggtggt
    13681 gacaaaactg tagttggtac tccagttgtt gaaggagcta ctgtcgttgg aactgttgaa
    13741 aaacaaggaa aacaaaagaa agttgtaaca ttcaaataca aacctaaaaa aggtagccac
    13801 cgtaaacaag gtcatcgtca accttacact aaagttgtca tcaacgcaat caacgcttaa
    13861 tttactagct tatgattaaa gcaattttta ctcgccaaaa aaatggtcaa ctgagcagtg
    13921 ttacactgac aggtcatgct ggtagtggta aacatggctt tgatattgtt tgtgcctctg
    13981 ttagtactct agccattaac tttgttaatt ctcttgaggt gttagcagat tgccaagcat
    14041 tagtagatct taatgatgtt gaaggtgggt atatggctat tacaatacca ccacatgaca
    14101 acaaggagga agttcaatta ttatttgaat cttttcttct aggaatgact agtttggcga
    14161 aggattcttc gaaatttgtc aacactcagg tcatctaaat ttaatttaag agaggaaaca
    14221 acattatgtt aaaaatgaat cttgctaact tgcaactttt cgcccacaaa aaaggtggag
    14281 gttctacatc aaacggacgt gactcacaag ctaaacgcct tggtgctaaa gctgctgacg
    14341 gtcaaactgt ttcaggtgga tcaatccttt accgtcaacg tggaactcat atctacccag
    14401 gtgtaaacgt aggccgtggt ggagatgaca cactttttgc taaagttgaa ggtgttgtac
    14461 gtttcgaacg taaaggacgt gataaaaaac aagtttctgt ttacccagta gctaaataat
    14521 tgtttaacat cgcattatat aaggctttcc aggtgtctgg aaagccgatt tttttatttt
    14581 ggtactcatg ttgctatcca tattttaaaa atcaatgtag gaagagagct tgtcagcagt
    14641 tttatctttt gaggatttag taacatgagc gtagatgttc atggtagttt ggatagtttc
    14701 atgaccaagt gtattggatt ttcattgttt gtttctatcc tactaaagat tgtagttgct
    14761 tatttagagc aatagttcta attgtaaaaa gtcagcataa taaaacaatg tcataagatg
    14821 ttgttataac gggctttatg gtataattaa ggatactcat tttaggaagg aaagcaatga
    14881 atattcaaca attacgctat gtggtagcca tcgctaataa tggaactttt cgagaagctg
    14941 cctctaaatt gtttgtcagt cagcctagct tatcagtttc tatcaaagat ttagaggcgg
    15001 aattagggtt tcaaattttt aatcggacta cttcaggaac agtcttgaca agtcaaggac
    15061 tagtttttta tgagaaagct ttagaagtag tcaaatcttt tgattctttt gaaaaaacct
    15121 tttctcaagc tgatctggat caaaatgaat tttctattgc tagccaacat tatgactttt
    15181 tgccacctct tattacagct ttttcgcaac aatacgatgg tcatcgtgtc tttcgtatct
    15241 ttgaatcaac aactattcaa attttagatg aggttgctca aggaaacagt gaaattggta
    15301 ttatttactt gaatgtagac aatcaaaaag gtctctttca gcggatggat aagctgggct
    15361 tggaatatgt ttctctaatt cctttcacta ctcatattta tctttcaaaa acgcatccat
    15421 tggctaatcg cgaagcgctt tatttgagcg atattcaagg attacctgcg gtaaggttta
    15481 cccaagaaag ggacgagtat ttgtattatt ctgaaaattt tgtggatact agtgaatgcc
    15541 ctcgtattta taatgtgtca gatagggcaa ccttgaacgg tattttagag agaaccaatg
    15601 cctttgcgac aggatcaggt tttctcgatc atcggagtgt taatgggatt aaagtgatac
    15661 ctctagctga tcatattgac aatcaaatga tttatgtcaa acgaaaagat aaaaaccttt
    15721 ctgttgctgg ggccactttt gtgactattt taaaagacta ttttgataaa agaaggaagg
    15781 ccttaacatg aaaaaacgat tgtttgtgct tagcttgatc ctccttgtag ctttggatca
    15841 acttagtaaa ttttggattg tttctcatat agcgcttgga gaagtgaaac cctttatccc
    15901 aggtatcgtc agcttgactt acttgcaaaa caatggggcc gccttttcca tattgcagga
    15961 ccagcaatgg ttctttgttg tcataacggt tttagttatc ggttatgcta tttattacct
    16021 tgctactcat ccccatttaa atatctggaa acaattagct ctcttgctta ttatttctgg
    16081 tggaatcggg aattttattg atcgtttgcg tttagcttac gtgattgata tggttcattt
    16141 agactttgtg gattttgcca tttttaatgt ggcagattca taccttaccg ttggtgtcat
    16201 attattagtg atatgtttat ggaaagaaga ggattatgga aattaatgtg ataacatcgg
    16261 ggcaacgctt ggataaggca ctagctgatt taagtcccct gtcacgtggt caagccaatg
    16321 accaaattaa gcaaggcctt gttttagtta atggtcagca aaaaaaggct aaatatacgg
    16381 ttcaagcagg agatgtgatt tgttttgaac tccctaaaga agaagtctta gagtaccaag
    16441 ctcagaatat tcccttggac attatctatg aagatgatac tctcgccatt attaacaagc
    16501 cacagggaat ggtagttcac ccttcggcgg gacacccttc aggaacaatg gttaatgcct
    16561 tgatgtatca tatcaaggat ttgtcctcaa tcaatggtgt ggttcgtcca ggaattgttc
    16621 atcggattga taaggacacc tcaggtctct taatggtagc taaaacagat gcagctcatc
    16681 aagccttggc agaagaatta aaggctaaaa aatctctcag aaaatacctc gccattgtcc
    16741 atggaaacct accgaatgat cgcgggatga ttgaagctcc tataggtcgt agcgaaaagg
    16801 accgtaaaaa acaggctgtt acagcaaaag gcaaagaagc agtgacacgt tttacagtgc
    16861 tagaacgttt tggcgattac agtctagtgg aattacaact ggaaactggt cgaacgcacc
    16921 aaattcgtgt tcatatggcc tatattggtc atcctgttgc tggggaccct ttatatggac
    16981 cacgtaagac actatcaggt catggccaat ttcttcatgc aaaaacattg ggattaacgc
    17041 atcctatgac aggaaaagaa atgatattta ctgttgaagc tcccgaaatt tttcaaaagg
    17101 tgttaaagct gctaagaaaa aaataaacag cgaggagtag ccattatttt tagtcttata
    17161 tctttgtgta aagtttttaa cattataaaa aatactaaaa agcatttacg ataagccagg
    17221 aaatatgaga agtcttttat gttattggga tatgcaacat atctgttaga gatctttatt
    17281 taaactctta aagaaaagag atattccaaa tagatgctag gtattgtctt ttttacttgt
    17341 ttttcttttc tttttttgct atactgattg aaataaaata ttcctttaaa acagtcctgt
    17401 gaggctgtca aggagacgga ttaaaaacat gcactatgat acttaattgt gctatatttt
    17461 gctgaatctc cttgtttcca aggagatctt tttatttgaa tttttagagg aggtttcaac
    17521 atgaaaacta aggaaattgt tgatgacgtg accatgaaac gcgccattac ccgtattact
    17581 tatgaaatta ttgaacgtaa taaacaatta gacaatgtgg ttttggcagg catcaaaacg
    17641 agaggtgttt ttctagctcg tcgcattcaa gaaagactgc atcaattaga agggttggat
    17701 ttaccaattg gtgagttaga catcaagcct tttagagatg acatgagagt tgaggaagac
    17761 acaactctga tgtcagtaga tattacaggc aaggatgtga ttttgattga tgatgtgctt
    17821 tatactggtc gtaccatccg cgcggccatc gataacttag tgagtttagg tcgaccagcc
    17881 cgtgttagtc tagctgtttt ggtggatcgt ggccatcggg aattaccgat tcgcgcggat
    17941 tacgttggga aaaatattcc aacaagcagt gtagaagaaa ttgttgttga ggttgtcgaa
    18001 gttgatggcc gtgatcgtgt tagcattatt gacccaacat aagaaggagg ttttactgtg
    18061 aaagatgtca tttatgatgt ggaagaagtg ccaaaggcag gtatgttagt tggtttgtcc
    18121 tttcagcatt tatttgccat gtttggggcg acggttcttg ttcctatttt agttggaatt
    18181 gacccgtctg tggccctcct atcgagtggt ttaggaacac ttgcacattt atcggtaacc
    18241 aaatttaaaa ttccagccta catgggctca agttttgctt atatagcagc tatgcagtta
    18301 ctgatgaaga caaatggtat tggtgctgta gctcaagggg ctatgacagg aggtctcgtt
    18361 tacctgattg tagctttgat tgttaaagtt attggcaatg attggattga taagatttta
    18421 ccaccgattg tagttggtcc gattgttatg gtcattggtc taagtctagc ttctacagct
    18481 gttaatgatg tcatgttaaa aaatgggaac tataatctga cttatcttgt tattgggtta
    18541 gtcaccttat tatcagttat ttttttcaat atttatggta agggaattgt tgctattgta
    18601 ccgcttttat tagggctatt agttggttat gttgtagccc ttttagtggg tgttctcacc
    18661 ggtcaagaaa ttgttgattt taccaatgtg gctcaggcca aatggtttag tatcccatca
    18721 gtggaaattc ctttcttaac ctacggggtt aaattctacc caagtgccat tttaactatg
    18781 gcacccattg cctttgttac aatgacagaa cattttggac acattatggt cttaaatagt
    18841 ttgacaaaaa gagattattt caaggatcca ggacttgaaa agactttaac tggtgatggt
    18901 tttgcgcaaa tcattgctgg cttcttaggg gcacctccgg tcacttctta tggtgaaaac
    18961 atcggtgtaa tggctctgaa taaaatattc tcggtctatg ttattgcagg tgccgcagtg
    19021 attgccgctc tcctcagttt tatcggtaag gtatcagcct taattcaatc tattccaaca
    19081 ccagttattg gaggtatatc agttgccttg tttggtgtaa tagcttctag cggtttgaaa
    19141 atcttgattg aatccaaagt tgatatggat aataagaaaa acttattgat tgccagtgtc
    19201 attttggtct ctgggattgg aggattgatg cttcaagtaa atggtcttca aatttcaggt
    19261 gtcgctttct caacacttct tggcatcatc ttatatcagg tacttcctga aaaataggaa
    19321 gaattgttgt tagaccactt acatagtcat cagtcgttag tgataaaggg gataatcatg
    19381 tcagttgtaa ataatcgcgt tgcacttacc aatttagttt caatggaagc attaaccacc
    19441 gaagaagttt taggtcttat caaccgtggt tcagaatata aggctggtaa ggttgttatt
    19501 tcggatcatc aaaaggatct tgttgccaat ttattctttg aaaactccac ccgaacccat
    19561 aagtcttttg aagtggctga gaagaaactc ggcttgacag ttcttgattt caatgctgat
    19621 gcaagtgcgg ttaacaaggg agaaagtttg tatgatactg tgttaaccat gagtgcactt
    19681 ggaacagaca tctgtgtgat tcgtcaccca gaagatgatt attacaagga attagtagaa
    19741 agtccaacta ttacagcttc cattgtcaat ggaggagatg gttctggaca acacccgagt
    19801 caatgtttac ttgacttatt aaccatctat gaagaatttg gacgctttga aggcttaaag
    19861 attgcgatcg ctggtgactt aactcactct agggtggcaa agtccaacat gcaaatcctc
    19921 aaaagattag gagcagagct ttatttctat ggacctgaag aatggtattc agaagccttt
    19981 aatgcttacg gcacttatat tgctattgac cagattatta aggaattgga tgttcttatg
    20041 ttgctgcgtg tacagcacga acgtcatgac ggccatcaga gtttttcaaa agaaggttac
    20101 catcaagcgt ttggattgac acaggaacgt taccaacaat taaaagattc tgctattatt
    20161 atgcatccag ctccagttaa tcgggatgtg gaaattgcag atagtttggt ggaagctcct
    20221 aaagcgagaa ttgtatctca gatggctaat ggtgtctttg ttcgcatggc tattattgaa
    20281 gccattttga atggtagaaa taaaaactca tgatgaaaga ttaaagcatg tttagaaggt
    20341 aggaaaaggt aggaaaatga caaaacgatt acttatttta gaagacggca ctatttttga
    20401 aggagaaccc tttggtgctg atattgatgt gactggtgaa attgtcttta atacaggaat
    20461 gacaggctac caagaatcca tcactgacca atcctacaat gggcaaattt taacctttac
    20521 ctatcctttg attggaaatt atgggattaa tcgagatgac tatgagtcca tttcacccac
    20581 ctgtaaaggg gttgttgtgt ctgaagtcag tcggttggca agtaattggc gcaagcaaat
    20641 gacgctagat gcgttcttga aaataaaagg gattccaggg atttcgggaa ttgatacgag
    20701 agctttgacc aaaattattc ggcaacacgg taccatgaaa gctacgatgg cagatgatgg
    20761 agatagtatc cagcatttaa aagatcaact gcgtgcaaca gtcttaccga ctaacaccat
    20821 tgaacaagtg tcaactaaaa cagcttatcc tgcaccaggg atcgggaaaa atattgtatt
    20881 ggttgatttt ggtttaaaac attctatttt aagagaattt tctaaaagac aatgtaatat
    20941 caccgttgtc cctttcaaca tcactgcaga agaagtgctc caactaaatc cggatggttt
    21001 gatgctatct aatggcccag gaaacccaga agatttgcca gaggctttag acatgattcg
    21061 tggggtacaa ggtaagattc ctatttttgg tatttgtatg ggacatcaac tctttagtct
    21121 agcaaatggt gccaagactt gtaaaatgac ctttggtcat cgtggcttta accatgccgt
    21181 tagagaaatt gcaacgggaa gaattgattt taccagtcaa aaccatggtt atgccgttga
    21241 acgtagtagt ctacctgata ctttaatggt tacgcatgaa gacattaacg ataagacggt
    21301 cgaaggagtg aaacatagag attttccagc tttttcagtc caatttcacc ctgatgcggc
    21361 tccaggacct catgatgcca gctacttatt tgacgaattt ttggaaatga ttgattcttg
    21421 gagatgtacg agtaaatagt aaatcagttg caatttcacg actatatcac ctcttgttac
    21481 tctatttatt aacatttaca gttgatttag taccctatca aaaatattat ttaatgttgc
    21541 tctgtgaggc aacaaataga gaaaggaagg caaggagaac acaagtaaat aatttagtaa
    21601 aaagttaggt tttcaaagag ataaagctct tattgagaag tgctgacttt accgcttctt
    21661 gtggtattct tggtatcatt aattatgcca aaacgaaaag atattcaaaa aatcatggtt
    21721 atcgggtctg gcccgattat tattggacaa gctgctgaat ttgattatgc agggactcag
    21781 gcctgtttgg ctctaaaaga agaaggctat aaggtcatac ttgttaattc aaatcctgca
    21841 acgattatga cagataaaga aatcgctgat aaggtatata ttgagccttt gactcttgaa
    21901 tttgttaatc gtatcattcg aaaagaaagg ccagatgcca tactgccaac cttaggtggt
    21961 caaacgggcc tcaatatggc aatggcatta tctaaagctg gtattcttga tgatttagag
    22021 attgaattgt taggtactaa gttatctgcg attgatcagg cagaagacag agaccttttc
    22081 aaacaattaa tgcaagaact agaccaaccc attccagaat ctacaattgt taaaacagtt
    22141 gatgaagctg ttacatttgc tagagatatc ggttatccag tcattgtgcg cccagctttt
    22201 actcttggtg gaactggtgg aggaatatgt agtagtgaag aagaactttg tgaaattact
    22261 gaaaacggcc taaagttatc accggtcaca cagtgtttga ttgaacgctc aattgctggc
    22321 ttcaaagaaa ttgaatacga agtaatgcga gatagtgctg ataacgcgct ggttgtttgt
    22381 aatatggaaa actttgatcc cgtagggatt cacacaggag attctattgt atttgcacca
    22441 acacagaccc tttctgatat tgaaaatcaa atgttacgtg atgctagttt aaaaataatt
    22501 cgagctctga aaattgaagg tggctgtaac gtccagttag cactagatcc ttatagtttc
    22561 aaatattatg ttattgaagt aaatcctaga gtgtctcgtt cctctgccct tgcttcaaaa
    22621 gcaacaggtt atcccattgc caaattggct gcaaagattg ctgttggcct aacacttgat
    22681 gaaatgatta accctatcac aggcactact tacgctatgt ttgaacctac ccttgattac
    22741 gttgttgcta aaattccgcg ttttccgttt gataaatttg aacatggtga acgccaactg
    22801 ggaactcaaa tgaaagcaac aggtgaagtc atggctattg gccgtaattt ggaagagtcc
    22861 ttactaaaag cctgtcgttc tttagaaatt ggtgtttgcc ataatgagat gacaagttta
    22921 agtaacatta gtgatgaaga gttagtgact aaagtgatta aagctcaaga tgatcgactt
    22981 ttttacttgt cagaagcaat acgacgaggt tatagtatcg aagaacttga gagcttgacc
    23041 aagattgatt tatttttctt ggataaatta cttcatattg ttgaaataga gcaagatcta
    23101 caaatgcatg ttgagcattt ggaaagcctc aaaaaagcaa agcgttatgg attttcagat
    23161 caaaaaattg cagaaatttg gcaaaaggat gaaagtgata tcagagcgat gagacattct
    23221 catagcctgt atcctgtcta taaaatggta gatacttgtg cagctgaatt tgatgctaaa
    23281 accccttatt tttattcaac atatgagttg gaaaatgaat ctgttcagtc gaataaagaa
    23341 tcaattttag ttttaggttc aggccctatt cgtattggtc aaggggttga atttgactac
    23401 gcaacggttc attctgttaa agccatccaa aaagctggct atgaagccat tatcatgaat
    23461 tcaaatccgg agactgtatc tacagatttt tcggtatcag ataaattata ttttgaaccg
    23521 ttaacctttg aagacgtcat gaatgtgata gatttagagc agcctaaggg agtaatagtt
    23581 cagtttggag gacaaactgc tattaactta gcacaagcac tttctgaggc gggtgttaca
    23641 atattaggaa cacaagtaga ggatttggat agggctgaag acagagattt atttgaaaag
    23701 gcgttgaaag agcttggaat cccacaacca caaggtcaaa cggcaactaa tgaagaagaa
    23761 gcattagagg cagctaaaaa aattggtttt ccagtcttag ttagaccttc ttacgtactt
    23821 ggcggccggg ctatggaaat tgttgaaaac aaagaagatc ttagagaata tataagaaca
    23881 gcggttaaag caagtcctga acatcctatc ttagtggact cttatatttt cggtaaggag
    23941 tgtgaagtgg atgccatttc agatggaaaa tcagtcttaa ttcctggtat tatggagcac
    24001 atcgaaagag caggtgttca ttcaggagat tcaatggcag tttatccgcc acagcaatta
    24061 tctaagcaaa ttcaagaaac aatagctgag tatactaaac gtttagccat tgggttaaat
    24121 tgtattggta tgatgaatgt tcaatttgtt attaaaaatg aacaagtcta tgttattgaa
    24181 gtgaatccac gcgctagtcg taccgtgcct ttcttatcaa aagtaacagg tattccgatg
    24241 gctcagattg ctactaagtt gattttaggt caaacattaa aggatctagg atatgaagat
    24301 ggtctttatc cccaaagtca actggtgcat atcaaagcgc ctgtgttttc atttacaaag
    24361 ttggcacagg ttgatagtct tttaggtcct gaaatgaaat caactggaga agtaatgggt
    24421 tcggatacta gtcttgaaaa agcactttat aaagcgtttg aagccaataa ttctcattta
    24481 tcagaatttg gtcaaattgt ctttactata gcagatgata gtaaggcaga agctctaagt
    24541 ctagcaagac gatttaaggc gattggctat caaattatgg ctactcaagg tacagcagct
    24601 tactttgctg agcaaggctt aagcgcgtgt ttagttggaa aaattggtga tgctgccaat
    24661 gacatcccga cactcgtgcg acatggtcac gttcaggcaa ttgttaatac agttggaatt
    24721 aaacgcacag ctgataaaga cggacagatg attagaagtt cagctattga acaaggagtt
    24781 cctttattta cagctttaga tactgcaaaa gctatgttaa cggttttaga aagcagatgt
    24841 tttaacatcg aagcgatata aaaattaaaa aatcattttt aagaaaactt aaaaatgatt
    24901 tttttagatt ataatgacaa aagtttcagt aagctattaa tttgtcatag catataagtc
    24961 attatcttgt ggcttgtttt cgactaaggg ttaagaattt cttgacaaca atataaaaag
    25021 gtcatatact tgtcttatac aaatagtaca aaaaggggag gatagaaatg tctaaaagag
    25081 gaaaaattaa aataacaacg aaaacaaagc ttattacagc tagtgttata acgctagtat
    25141 taattataac tggagtagtc ttgtggaaac aacaacaaaa tacgctaaca gctgatatcg
    25201 ctaaagaacc ttactctact gttagtgtaa ctgaagggag tattgcttct tcgactttac
    25261 tctcaggtac tgtaaaggct ttatcagagg aatatattta tttcgatgct aataaaggaa
    25321 atgatgcaac tgttacagtt aaagtaggtg atcaggtaac gcagggccag caattagttc
    25381 aatataatac aacaacagct cagtcagctt atgatactgc tgttaggagt cttaacaaga
    25441 ttggccgaca aattaatcat cttaaaacat acggagttcc tgctgttagt acagaaacta
    25501 ataaagatga agctaccggt gaagagacga caacaacagt tcaaccatca gctcagcaaa
    25561 atgctaatta taaacagcag ctgcaagatt taaatgatgc ttatgcagat gcacaagcag
    25621 aagtaaataa agcgcagata gcgttaaatg atacagtagt tatcagtagt gtctctggaa
    25681 ctgttgtgga agtaaataat gatattgatc cttcttcaaa gaacagtcaa acacttgttc
    25741 acgtagcaac cgaaggacag cttcaggtga aaggaacatt gacagagtat gatttagcaa
    25801 acgttaaggt tggtcaatct gtaaaaatta agtctaaagt ttattctaat caagaatgga
    25861 ctggaaaaat atcatatgtt tcaaactatc caactgagtc taatgcaggt tcaacaacgc
    25921 cagcaggtag cactggagcg ggaagttcta caggagctgc ctatgattac aagattgata
    25981 ttataagtcc tctcaaccag cttaaacaag gtttcactgt ttctgttgag gttgttaatg
    26041 aagctaaaca ggccttagtt cctttaacgg ctgttattaa gaaagataaa aaacactatg
    26101 tttggactta tgatgatgct actggcaaag ccaaaaaagt agaggtgaca cttggaaacg
    26161 cagatgcaca acaacaagaa attcataaag gagtagctgt tggtgacatt gttattgcca
    26221 atccagataa aaatatcaaa ccggataaaa aactagaagg ggttatttca ataggtacca
    26281 acacaaaacc ggaaaaagat tctcaatcaa agaataaaaa atcaggggtg gataaatagt
    26341 gtcgcatgaa aaaaaacaat taatgcaatt atcaaatatt gtgaagagct atcaaaatgg
    26401 ggatcaagtt cttaaagttt tgaaaggtat taatttaaca gtatatgagg gtgaattttt
    26461 agctattatg ggtccgtctg gctcgggaaa atcaacacta atgaatatta ttggtttatt
    26521 agatcggcca acatccggag attacacatt acataatact aaaatagaaa tccttaatga
    26581 tcgtgaatta gcaaaagtac gaaacgatga aatcggcttt gtctttcaac aattctttct
    26641 gttagctaaa ttaactgcat tacaaaatgt tgagttgcct cttatttatg ctggtgtaaa
    26701 cgtctcaaaa agacgtgaac aggctaagca atttcttgaa aaagtaggac ttggacgacg
    26761 tatcaaacat ctaccgtcag aactgtcagg aggtcaaaag caaagggtgg ctatcgcacg
    26821 agctttagta aatgacccat ctattatttt agcggatgaa ccaacaggtg cactagatac
    26881 taaaacaggt cagcaaatta tggaactctt gacagagtta aacaaagaag gtaaaacaat
    26941 tattatggtt actcatgaac cagaaattgc tgattttgca actcggaaaa ttattattcg
    27001 tgatggtgat atcacaacgg atactacagc tagtgttgtt attgactagg aggaaaaata
    27061 atggaaaatt ggaaattcgc tcttagctca atttgggggc ataagatgcg ctcaatcctg
    27121 acaatgttgg gaattatcat cggtgtagca gcagttgtga ttattatggg gttgggaaat
    27181 gctatgaaaa atagtgtaac aagcacattt tctagcaagc aaaaagatat tcaactctat
    27241 tttcaagaaa aaggagaaga agaagacctg tatgccggat tgcacacgca tgaaaataat
    27301 cacgaagtga agccagagtg gttagaacaa attgttaaag atatcgatgg tattgattct
    27361 tattacttta ccaattctgc tacttctact atttcgtatg aaaaaaagaa agtagacaat
    27421 gcttcgatca taggggtaag taaagattat tttaacataa aaaactatga tatcgtagct
    27481 ggtcgtacac taacagataa tgattattct aatttttcgc gtatcattct tttagataca
    27541 gtattagccg atgacctatt tggcgaaggt aattataagt cagctttaaa taaagtggta
    27601 agtttatctg ataaagatta tttagttatt ggtgtttata aaactgatca gacgccagtt
    27661 tcttttgatg gattatcagg cggcgctgtc atggcaaata ctcaggtagc ttcagagttt
    27721 ggcaccaaag aaataggatc tatctacatt catgtgaacg atattcaaaa cagtatgaac
    27781 ttaggaaatc aagcagctga tatgctaacc aacatatcgc atattaaaga tggacagtac
    27841 gctgttcctg ataatagcaa aatcgttgaa gaaatcaata gtcagttcag tatcatgaca
    27901 acagtcatag gttcaattgc agcgatctct cttttagtgg gaggaattgg tgttatgaat
    27961 attatgttgg tatctgtcac agagcgaact agagaaattg gtttacgtaa agcacttggt
    28021 gcaacacgcc tgaaaatttt atcacaattt ttgattgaat ccgttgttct aacagttctt
    28081 ggaggcttga taggattatt attagctcaa ttaagtgtag gggctttagg aaatgcgatg
    28141 acattaaaag gagcttgtat ttctcttgat gtggccttaa tagcagtttt attttctgcc
    28201 tcaataggtg tcttttttgg aatgttgcca gctaataaag ctagtaagtt agatccaatt
    28261 gaggcattac gttatgaata gaatagttcg tatcaattaa tctatagacg ctgactagtt
    28321 taacgtctga ttatatctag tttttgtatt agcacagtag ttatatcatt gaagtgttag
    28381 tgcatttttt gaggtgataa tatctagcaa agctatcatt ggtatgatga tgtggatagc
    28441 gtataagtga tacatctctt atttctagct gaacttcggt tcggctagga agttgtcagg
    28501 caatgagttg tttaaaataa aggagattag ttcaatgaca tttttatctg atttgatatc
    28561 attaatgaca aaaatcagat tatcttgggt aataaaggcg ggtatttttc aattattatt
    28621 tgtaacgatt gctaatattg tcttatcaga atttttctat tttatattag acgttactgg
    28681 tcaatatcat ttagataaag acaatgttgt gactttttta aaaaatccta tagcacttgc
    28741 tttattaggt gcctatttat ttttattagc tgcttttatt caccttgagt tttttgctct
    28801 atatcgaatt attgcggatc aagaaattag tttctatctt tttagaaaac agttttctta
    28861 ttacctaagg gggctttgga aaacattttc tggttaccaa ttattacttt ttttgcttta
    28921 tatcctattg actattccag tcttacatat tggtttatct tctgtgatta ctcaaaagct
    28981 ttatcttcca gaatttattg ttggggaatt atcaaagata actagcacaa agtacttgct
    29041 ttatggcagt cttattcttg tattttacct taacctaaga ttagtatatt ttttaccatt
    29101 gatagcaatc aaccatcgta cggttgctca agcatggaga gagagttggc aaaagactaa
    29161 aaagaaacat gtattgttat ggatgaaact ttttgcaatc aatggtctta cgcttgtagt
    29221 cgtatcgcta gctatttcca tgattcttat ttttgttgat atgtttaatc ctaaggggaa
    29281 taatattatt gttcagctgg gagctttgac ctttacatgg gagctcattt tttttactac
    29341 tatttttttt aaactctgtt cagcaatgat tttaaaagag gcaattgaac cacaaaagca
    29401 atatgatgag ccaagaagaa gtaataaggc atatgttgta atctttatcg tggtgacagt
    29461 aggttttgct tatcaatctc ttgaacgttt aacttttttt gacacatctc actctaagac
    29521 agttatcgcg catagaggac ttgtatcagc aggtgtagaa aattctctgg aagcccttga
    29581 aggtgctaag aaagcaggaa gtgattatgt agaactggat ctaatcttga ctaaggataa
    29641 tcactttgtg gtgtctcatg ataatcgatt gaagcgttta gctggagtaa ataagacgat
    29701 tcgcaactta accttaaaag aagttgaaca tctaacgagt catcaaggac atttttcagg
    29761 gcgttttgtt tcttttgaca ctttttatca aaaggctaag aagttgaata tgccattact
    29821 tattgaactc aagccaattg gtacagaacc tggaaattat gtcgatttgt ttttagaaac
    29881 ttatcatcga cttggtataa gcaaagataa taaagtcatg tctttagatt tagaagtaat
    29941 agaagctatc aagaaaaaaa atccatcaat tacgactggt tatatcatac caattcaatt
    30001 tggctttttt ggagatgaat ttgttgattt ctatgtcatt gaagactttt cttatcggtc
    30061 ttatttgtcg tcccaagctt tttggaataa taaagaaatt tacgtttgga ctattaatga
    30121 tcccaagcgc atagagcatt atctcctaaa gcctattcag ggaattatta cagatcaacc
    30181 agctttaact aatcaattga ttaaagactt aaaacaagat aattcttatt ttag
//
LOCUS       DATXQK010000018        28778 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.018, whole genome shotgun sequence.
ACCESSION   DATXQK010000018 DATXQK010000000
VERSION     DATXQK010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28778
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.018"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            37..885
                     /locus_tag="VC394_000895"
     CDS             37..885
                     /locus_tag="VC394_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEP5994373.1"
                     /translation="MRRKIKPIVVLVFFILLAMVLIIGKRQANHAKQKEVEDAKSHIP
                     IATSNPSKAKTSTSETEDFILNPIVDVSGWQLPEEIDYDTLSRHISGAIVRVYGGSQI
                     TAHNNAAFTTGIDKSFKTHIKEFQKRNVPVAVYSYALGRSTKEMKEEARAFYKNAAPY
                     NPTYYWIDVEEATMKDMNKGVTAFREELKKLGAENVGLYIGTYFMAEQDISTKGFDAV
                     WIPTYGSDSGYYEAAPNTTLDYDLHQYTSQGYLSGFNNALDLNQIAVTKDTKKTFEKL
                     FGNSNN"
     regulatory      1013..1109
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1154..1717
                     /gene="thiT"
                     /locus_tag="VC394_000896"
     CDS             1154..1717
                     /gene="thiT"
                     /locus_tag="VC394_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEP5994374.1"
                     /translation="MSPNTNVKYLIEAAIFAALAMALSFIPDFAGWFSPSYGAIALVI
                     FSLRRGLKYGMLAGLIWGLLHFVLGKVYYLSMSQVFIEYILAFTSMGLAGSFSDSLIK
                     TLRRQQTFFAVFLAIMASLLAVTVRYLWHFLAGIIFWGSYAPKGMSAVWYSFSVNGTA
                     GVLTFLITCLALMIALPIHPQLFDPKD"
     gene            1807..2286
                     /locus_tag="VC394_000897"
     CDS             1807..2286
                     /locus_tag="VC394_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEP5994375.1"
                     /translation="MAKKTKFKKTLVEQILDKANIAHQGLKLNALEGDFPDDLQPSDI
                     YKTLALTGDQTGPLIGIIPLTEHLSEKQLAKVSGNKKVSMVPQKDLQKTTGYIHGANN
                     PVGIRQKHSYPIFIDQTALEKGQIIVSAGEVGRSIKISSQALADFVGASFADLKKRK"
     gene            2627..3250
                     /locus_tag="VC394_000898"
     CDS             2627..3250
                     /locus_tag="VC394_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEP5994376.1"
                     /translation="MKLYFVRHGKTLWNLEGRFQGAGGDSPLLEEAKDEIHLLGKELA
                     KVAFDAVYTSDLQRAMATAAIILDAFDQQPKLYHTDQLREWRLGKLEGAKIATMAAIY
                     PQQMLAFRENLAQFKSDQFEAESIYQTTQRVCHLIQSFKDKHYQNVLIVGHGANLTAT
                     IRSLLGFEPALLLAKGGLDNTSLTILETKDYLTYDCLIWNDKSFLEV"
     gene            complement(3358..4260)
                     /locus_tag="VC394_000899"
     CDS             complement(3358..4260)
                     /locus_tag="VC394_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEP5994377.1"
                     /translation="MITAIVFDVDDTIYDQQAPYRIAMEKCFPDFDMSVMNQAYIRFR
                     HYSDVGFPRVMAGEWTTEYFRFWRCKETLLEFGYREIDEAAGVHFQEVYEHELENITM
                     LDEMRMTLDFLKSKNVPMGIITNGPTEHQLKKVRKLGLYDYIDPKRVIVSQATGFQKP
                     EKEIFNLAAEQFDMNPQTTLYVGDSYDNDIMGAFNGGWHSMWFNHRGRQLKPGTKPVY
                     DVAIDNFEQLFGAVKVLFDLPDNKFIFDVNDKKNPILQMGINNGLMMAAERLLESNMS
                     IDKVVILLRLTKQQEKVLRLKYAR"
     gene            4434..5927
                     /gene="lysS"
                     /locus_tag="VC394_000900"
     CDS             4434..5927
                     /gene="lysS"
                     /locus_tag="VC394_000900"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEP5994378.1"
                     /translation="MSNQHIEELNDQQIVRREKMTALAEQGIDPFGKRFDRTANSAEL
                     KEKYADKTKEELHELNETAIVAGRLMTKRGKGKVGFAHLQDREGQIQLYVRKDSVGED
                     NYEIFKKADLGDFIGVEGEVMRTDMGELSIKATKLTHLSKSLRPLPEKFHGLTDIETI
                     YRKRHLDLISNRESFDRFVTRSKMISEIRRYLDGLDFLEVETPVLHNEAGGAAARPFV
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADYLDIMNLTEGIIQHAAKAVKGDGPIDYQGTEIRINEPFKRVHMVDAIKEVTGVD
                     FWPEMTVEEAIALAKEKQVPLEKHFTSVGHIINAFFEEFVEETLVQPTFVFGHPVEVS
                     PLAKKNPEDTRFTDRFELFIMTKEYANAFTELNDPIDQLSRFEAQAQAKELGDDEATG
                     IDYDFVEALEYGMPPTGGLGIGIDRLCMLLTNTTTIRDVLLFPTMKP"
     gene            6063..6203
                     /locus_tag="VC394_000901"
     CDS             6063..6203
                     /locus_tag="VC394_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994379.1"
                     /translation="MTKEYISYISAAIFLLYGLLAAKWIFLGLGLILVLVGLADRLNS
                     KK"
     gene            complement(6300..6512)
                     /locus_tag="VC394_000902"
     CDS             complement(6300..6512)
                     /locus_tag="VC394_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEP5994380.1"
                     /translation="MAEFTFNIEEHLLTLSENDKGWTKELNRVSFNGAEAKWDIRTWS
                     PDHTKMGKGITLTNEEFKTILDAFRK"
     gene            complement(6728..8014)
                     /locus_tag="VC394_000903"
     CDS             complement(6728..8014)
                     /locus_tag="VC394_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEP5994381.1"
                     /translation="MSHMKKRPEVLSPAGTLEKLKVAIDYGADAVFVGGQAYGLRSRA
                     GNFSMEELQEGIDYAHARGAKVYVAANMVTHEGNEIGAGEWFRQLRDMGLDAVIVSDP
                     ALIIICSTEAPGLEIHLSTQASSTNYETFEFWKAMGLTRVVLAREVNMAELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCVLSNHMSHRDANRGGCSQSCRWKYDLYDMPFGGERRSL
                     KGEIPEDYSMSSVDMCMIDHIPDLIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     MESPEAFYAIKEELIDELWKVAQRELATGFYYGIPTENEQLFGARRKIPQYKFVGEVV
                     AFDSASMTATIRQRNVIMEGDRIECYGPGFRHFETVVKDLHDADGQKIDRAPNPMELL
                     TISLPREVKPGDMIRACKEGLVNLYQKDGTSKTVRT"
     gene            complement(8116..9042)
                     /locus_tag="VC394_000904"
     CDS             complement(8116..9042)
                     /locus_tag="VC394_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEP5994382.1"
                     /translation="MEKIIITATAESIEQVKALLAAGVDRIYVGEANYGLRLPHNFSY
                     DELRQIAKLVHDAGKELTVACNALMHQDMMDQIKPFLDLMIEIAVDYLVVGDAGVFYV
                     NKRDGYNFKLIYDTSVFVTSSRQVNFWGQHGAVESVLAREIPSAELFTLAENLEFPAE
                     VLVYGASVIHHSKRPLLENYYHFTKIDDEVSRERGLFLAEPGDASSHYSIYEDNHGTH
                     IFINNDIDMMSKLGELYAHGLTHWKLDGIYCPGDDFVAITKLFIQAKTLLEAGQFTQE
                     EAEKLDQAVHAHHPAGRGLDTGFYEFDPKTVK"
     gene            9241..9525
                     /locus_tag="VC394_000905"
     CDS             9241..9525
                     /locus_tag="VC394_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEP5994383.1"
                     /translation="MPAPLKKHNSVEQDDSYQESQSKFRDFQEFNGQSAKLKELVFFA
                     RIASFSLITVLFAFVLLVMNLAPMFAFLFASIISLGITSLFAKVIKSLLM"
     gene            complement(9685..10113)
                     /locus_tag="VC394_000906"
     CDS             complement(9685..10113)
                     /locus_tag="VC394_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEP5994384.1"
                     /translation="MNKSFKNLVIGAVSGVAAAYFLSTEKGKALKNRAEKAYQAYKES
                     PDDYHQFAKEKGSEYSHLARDTFYDVKDKLASGDLTKEDMLDLLKDKTTAFVQKTKET
                     LAEVEAKEKQDDVIIDLNEEDIIIDYTEQDEPVSDTLDKH"
     gene            complement(10106..10513)
                     /locus_tag="VC394_000907"
     CDS             complement(10106..10513)
                     /locus_tag="VC394_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEP5994385.1"
                     /translation="MDLVGISLMIIALAFVALVIFLIIVLKKVSETIDEAKKTISVLT
                     SDVNVTLHQTNDILAKANILVEDVNGKVATIDPLFVAIADLSESLSDLNSQARHFGQK
                     ATNATGNVSKAGKLALVGKVASKVFGKKGEKHE"
     gene            complement(10535..11314)
                     /locus_tag="VC394_000908"
     CDS             complement(10535..11314)
                     /locus_tag="VC394_000908"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEP5994386.1"
                     /translation="MINPIALKCGPLAIHWYALCILSGLVLAVYLASKEAPKKGISSD
                     AIFDFILIAFPLAIVGARIYYVIFEWSYYVKHLDEIIAIWNGGIAIYGGLITGALVLL
                     AYCYNKVLNPIHFLDIAAPSVMVAQAIGRWGNFINQEAYGKAVSQLNYLPSFIQKQMF
                     IEGSYRIPTFLYESLWNLLGFVIIMMWRRKPKSLLDGEIFAFYLIWYGSGRLVIEGMR
                     TDSLMFLGIRISQYVSALLIIIGLIFVIKRRRQKGISYYQE"
     gene            complement(11311..12243)
                     /locus_tag="VC394_000909"
     CDS             complement(11311..12243)
                     /locus_tag="VC394_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEP5994387.1"
                     /translation="MTVTVKMLVQKVKLDVVYATDNLLSKEITTSDISRPGLEMTGYF
                     DYYAPERLQLFGMKEWSYLTQMTSHNRYSVLKEMFKKDTPAVVVSRNLAIPKEMVQAA
                     KEEGISLLSSRVSTSRLAGEMSYFLDASLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDVYAKDEETLWGEPAEILRHLLEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAIYLENFEAGKVFDRLGNGNEEITFSGVRIPRIRIPVKTGRNVSVVIE
                     AAAMNHRAKEMGFDATKTFEDRLTQLITKNEVSQ"
     gene            complement(12608..12880)
                     /locus_tag="VC394_000910"
     CDS             complement(12608..12880)
                     /locus_tag="VC394_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEP5994388.1"
                     /translation="METKFYKQRKNRLVAGVIAGLADKYGWDLALARVLAALLIYGTG
                     FGVLLYILLAIFLPYKEDLLEERYGRGPRRRKDADVLNEEEDGWFW"
     gene            complement(12944..13381)
                     /locus_tag="VC394_000911"
     CDS             complement(12944..13381)
                     /locus_tag="VC394_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEP5994389.1"
                     /translation="MTLTNYVQEVSLADFGKPFHHKAYWNKRLKTTGGRFFPKDGHLD
                     FNPRMLEEHGELIFRKIVRHELCHYHLYFEGRGYHHKDRDFKDLLAQVNGLRYVPTSS
                     KSKTNHHYSCQTCGQVYQRKRRINLAKYVCGNCHGKLMEKNQS"
     gene            complement(13368..15500)
                     /locus_tag="VC394_000912"
     CDS             complement(13368..15500)
                     /locus_tag="VC394_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEP5994390.1"
                     /translation="MENNNNHNIAEALSVSLHQIEQVLALTAQGNTIPFIARYRKEVT
                     GNLDEVVIKSIIDMDKSLTTLNERKATILAKIEEQGKLTDQLRTSIEATEKLADLEEL
                     YLPYKEKRRTKATIAREAGLFPLARLILQNAQNLETAAEPFVTEGFASPQEALAGAVD
                     ILVEAMSEDAKLRSWTYNEIWQYSRLVSTLKDEQLDEKKVFQIYYDFSDQVSNMQGYR
                     TLALNRGEKLGILKVSFEHNLEKMQRFFSVRFKETNPYIEEVINQTIKKKIVPAMERR
                     VRSELSDAAEDGAIHLFSENLRHLLLVSPLKGKMVLGFDPAFRTGAKLAIVDQTGKML
                     TTQVIYPVAPASQTKIQAAKETLTQLIETYQIDIIAIGNGTASRESEAFVADVLKDFP
                     NTSYVIVNESGASVYSASELARHEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIG
                     VGQYQHDVSQKKLSENLGFVVDTVVNQVGVNVNTASPSLLAHVSGLNKTISENIVKYR
                     EENGALTSRADIKKVPRLGAKAFEQAAGFLRIPGAKNILDNTGVHPESYPAVKELFKV
                     LGIQDLDDAAKATLAAVQVPQMAETLAIGQETLKDIIADLLKPGRDLRDDFEAPILRQ
                     DILDLKDLEIGQKLEGTVRNVVDFGAFVDIGVHEDGLIHISEMSKTFVNHPSQVVSVG
                     DLVTVWVSKIDLDRHKVNLSLLPPRDTH"
     gene            15734..15889
                     /locus_tag="VC394_000913"
     CDS             15734..15889
                     /locus_tag="VC394_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994391.1"
                     /translation="MAITHKKNDELEKMLAGFASIPSFDKPLEVNTDGKLATKEKTTE
                     QTKPSKP"
     gene            16037..16939
                     /locus_tag="VC394_000914"
     CDS             16037..16939
                     /locus_tag="VC394_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEP5994392.1"
                     /translation="MSLFSNLPPSVLQWFAIFMSIIIEALPFVLLGTILSGCIEVFVT
                     PELVQKYLPKQKCLRILFGTFVGFVFPSCECGIIPIINRFLEKKVPSYTAVPFLATAP
                     IINPIVLFATYSAFGNSLRFLILRLVGAALVAITLGVMLAFIVDDNILKDNAQPVHFH
                     DYSHESLPKRIYLALVHAIDEFFDTGRYLVFGTLIASAMQIYVPTRVLTTIGHNPLTA
                     ILIMMLMAFILSLCSEADAFIGASLLSTFGVAPVLAFLLIGPMVDIKNLMMMVKTFKG
                     RFIVQFIGVSVLMIAVYCLLVGVL"
     gene            16939..17754
                     /locus_tag="VC394_000915"
     CDS             16939..17754
                     /locus_tag="VC394_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEP5994393.1"
                     /translation="MIRFLILAGYFELTMYLQLSGKLDQYINVRYSYLAYISMVLSFI
                     LALVQLYTWMKNIKVHSHLTGKIARLTSPFILVFPVLIGLLVPTVTLDSTTVSAKGYT
                     FPLAAGASKTGVSDDGTTIQYLKPDTSLYFTKSAYQKEMRQELHKYKGKKPVTITTEN
                     YMEVMELIYLYPDEFLDRDIQYTGFVYNEPGHDNYQFLFRFGIIHCIADSGVYGLLTT
                     GNQTSYPNNTWLTVKGRLHMEYDKNLEQHLPVLQLTEVHQTKEPNNPYVYRVF"
     gene            complement(17853..19277)
                     /locus_tag="VC394_000916"
     CDS             complement(17853..19277)
                     /locus_tag="VC394_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEP5994394.1"
                     /translation="MGIFPKDFLWGGAVAANQVEGAFEADTKGLSVQDVLPNGGLGEW
                     TDSPTSDNLTLEAIDFYHRYKEDIALFAEMDFKVFRTSIAWSRIFPNGDDDQPNEAGL
                     QFYDDLFDELLNYGIEPLVTLSHYETPLHLAKAYNGWTDRRLIGFFERFAQTVMERYK
                     DKVKYWLTFNEVNSILHMPFTSGGIMTEKEKLSLQDLYQAIHHELVASASVTKLAHEI
                     NPDVKVGCMILAMPAYPMTSDPRDILAAHAFENLNLLFSDIHVRGKYPSYIKSYFKEN
                     GIEIVFEDGDKELLAEHTVDFLSFSYYMSVTQAHNPEAYTSGQGNILGGLSNPYLESS
                     EWGWQIDPIGLRLVLNQYYDRYQIPLFIVENGLGAKDQLVQTADGSMTVHDDYRIDYM
                     SQHLVQVAKAIEDGVEVMGYTAWGCIDCVSMSTAQLSKRYGFIYVDRNDDGTGQLTRY
                     KKKSFDWYRQVIQTNGRYLEDNFS"
     gene            complement(19296..21158)
                     /locus_tag="VC394_000917"
     CDS             complement(19296..21158)
                     /locus_tag="VC394_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEP5994395.1"
                     /translation="MTYQETAKAILAAVGGKTNIQRVTHCVTRLRLVLKNDEKVKDQQ
                     VKAISNVIGVMRKNGQYQIILGNDVNNYYQAFLSLGHFDNQDEDHSSKAKGSILERLI
                     ETIAGVITPLIPALLGGGMLKVVGILLPMLGLASADSQTVAFINFFGDAAYYFMPVMI
                     AYSAAARFKVTPVLAATIAGILLHPAFVAMVAEGKPLTLFGAPVTPASYGSSVIPILM
                     MVYLMQYIEKWVNRLVPSVMKSFLQPTLIILISGFLALVVVGPLGVIIGQGLSNTMLA
                     IYHVAPWLALAILGAIMPLVVMTGMHWAFAPIFLAASVATPDVLILPAMLASNLAQGA
                     ASLAVAFKTKQKQTRQVALAAGISALLAGITEPALYGVTLKFKKPLYAAMISGGLVGA
                     FIGFVNIASYTFVVPSIIGLPQYINPSGGANFINALIAGAATIVLAFSLTWFMGIDEE
                     SPKQVSVAADMSQVKSGLSTKQTLYAPMTGEMLFLSEVPDETFSSKLLGEGFAILPSE
                     GVVYAPFDGEVITFFPTKHAVALKNTRGVEVLIHVGIDTVELKGQGFEQLVSVGDVVK
                     RGQALLKMDIDFITSKGYSLISPVVVTNSAEQLEIIIQDDKKMVTKEDALLVIL"
     gene            complement(21160..22002)
                     /locus_tag="VC394_000918"
     CDS             complement(21160..22002)
                     /locus_tag="VC394_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEP5994396.1"
                     /translation="MLIKRVLNHNAAISTNHQGLDILLMGKGIAFGKKVGDSIELNAI
                     ETSFVLKNSDNMNRFTELFITVPQEVVACSERIINLGKIKLGKTLDEILYINLTDHIH
                     SAIERHEQGMLIHNPLRWEIQRYYPDEYSLGVKALELIERNLGVTLAIDEAAFIAMHF
                     VNASLDTPFKEPHRLTEIVSYIEQKIKTDFKTELDDTSIDYYRFMTHIKLFAQRVLSE
                     MSYDDDDAELLLVVKTKYPKEYRCVLDISEEIKKRYNYHLNSSELLYLTVHVKRLVKH
                     LKET"
     gene            22330..23697
                     /locus_tag="VC394_000919"
     CDS             22330..23697
                     /locus_tag="VC394_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEP5994397.1"
                     /translation="MSHHQQTVSKQTIMAIIAIALIGFSGILSETSMNVTFPTLMSVY
                     QLPLNSLQWMTTIYLLAVAIMMTTSATLKKNVRERPLFFMATGLFTFGTILAVLTQSF
                     AIMLLARIFQGIGTGLVMPQMFNIILERVPMHKVGLFMGFAGLIISLAPAFGPTYGGF
                     MISHFSWQWIFICILPVPLIAGILAYYYLEDSPVSEKVPFDWLAFIALSISLTSALLA
                     ITSLENGSVNLYYLGLFILSFVLFLYKNLTAKQPFLDIRILKIPSLTFGLIPFFVFQL
                     INLGINFLTPNFIVMEKIANSSQAGMVLLPGTLLGALLAPAFGKLYDQKGAKLSLYLG
                     NALFSLSLIIMTLQTRHFMLLPFTLLYILFTFGRNMGFNNSLATAIRELPAEKNADAT
                     AIFQMMQQFAGALGTAMASLIANSQPEFTSGVQSVYLLFTIFALLDFIFFFAMFYHLG
                     KKGLA"
     gene            complement(23751..25301)
                     /gene="ftsY"
                     /locus_tag="VC394_000920"
     CDS             complement(23751..25301)
                     /gene="ftsY"
                     /locus_tag="VC394_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEP5994398.1"
                     /translation="MGLFDRLFGKKETPKVAEEKLEENLLTETTQKEELSEKANEQNK
                     IEVVQQEDVSSEGAGSVENGPEAASVNAQVEEETGDNSNHPSEDTNEFAADKTDLKVS
                     ELSQSTASEPKDLVDQPVVEQFPTKQAQADASNDSANEEAVDTSKEQSSSQQVMEDYY
                     RRKAALEKSLQEKAAATVPVMPEEVPQENQASTSAEASQNKATHDTIPETDQEKYKRS
                     LKKTRTGFSARLNSFFANFRRVDEEFFEDLEEMLILSDVGVHVATTLTEELRYEAKLE
                     NAKKPDALKRVIVEKLVDIYEKDGRYNEAINYQDGLTVMLFVGVNGVGKTTSIGKLAY
                     RYKQEGKKVMLVAADTFRAGAVAQLVEWGRRVDVPVITGPEKADPASVVFDGMEKAVA
                     KGVDILLIDTAGRLQNKENLMAELEKMGRIIKRVLPDAPHETLLALDASTGQNALSQA
                     KEFSKITPLTGLILTKIDGTAKGGVVLAIRQELDIPVKFIGFGEKVDDIGEFHSEDFM
                     KGLLEGIL"
     gene            complement(25301..26125)
                     /locus_tag="VC394_000921"
     CDS             complement(25301..26125)
                     /locus_tag="VC394_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEP5994399.1"
                     /translation="MNIRILALDLDGTLYNTEKIVTDANKKALAAAREKGVKVVITTG
                     RPLKAIGNLLEELDLLDHDDYSITFNGGLVQRNTGEVLDKSSLSFDQVCQIQQALEAV
                     GLPTDIISGGDVYSIPSKDGRHSQYHLANPLLTFIEVTSVAELPKDITYNKIVTVTDP
                     DFLDQQIIKLSPSLFEDFEAFKSRDIIFEIMPKGIDKAFGLNLLCQHLGLDARHVMAM
                     GDEANDFAMLEWAGLGVAMANGVSGAKAVADAVTTLTNDESGVAEAVKTFILEEES"
     gene            complement(26129..26926)
                     /locus_tag="VC394_000922"
     CDS             complement(26129..26926)
                     /locus_tag="VC394_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEP5994400.1"
                     /translation="MIKLIATDMDGTFLAEDGTYNQEQLAALLPKLAEKGILFAVSSG
                     RSLLAIDQLFEPFLDQIAVIAENGSVVQYHGEILFADMMTKEQYTEVAKKVLANPHYV
                     ETGMVFSGQKAAYILKGASEEYIQKTKHYYANVKVINGFEDMENDAIFKVSTNFTGHT
                     VLEGSDWLNQALPYATAVTTGFDSIDIILKEVNKGFGMEHLCQALGIKKAETIAFGDN
                     FNDYQMLEFAGRAIATENARPEIKVISDQVIGHCNDGAVLTYLKGLV"
     gene            27230..28356
                     /locus_tag="VC394_000923"
     CDS             join(27230..27464,27464..28356)
                     /locus_tag="VC394_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612023.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEP5994401.1"
                     /translation="MSTVKRYDEEFKQSLVNLYQTGKTQSELCKDYGVSASALAKWIK
                     QYSQVKLEDNSVLTAKQIQELQKRNAQLEEENLIPKKSKCHIHAKLKVRLLAVYRLRF
                     EHDTTTLCRVLRVNRSTYYKFLKHKPSKRELDNQIYRKQILEIYTKANKRLGVKSIKV
                     ILQRDYDTKISEGRIYRLMKNMALPKMATVKPKTALKRTQKTYPQNLLNQKFNPDKPN
                     QVWSTDFTYISIGYKKYVYLCAILDLYSRKCIAWKLSHHMDAKLACDTLELALNKRKI
                     EGTLLFHSDQGSQFKARKLRKIIDDNTIMHSFSKPGYPYDNAVTEAFFKYLKHRQINR
                     KKYQNIKQVQLDCFEYIENFYNNYNPHTANLGLTPNQKEEN"
BASE COUNT         8542 a         6142 c         5139 g         8955 t
ORIGIN      
        1 tgattttttt tagtaattag ggtacaataa cagttaatga gaagaaaaat aaaacctatt
       61 gttgtgctag tcttctttat tttactggca atggtattga ttatcggaaa aagacaagct
      121 aatcatgcga aacaaaaaga agttgaagac gctaagagtc atattcctat tgcaaccagt
      181 aatcctagta aagcaaaaac tagcacttct gaaaccgaag actttatttt aaatcctatt
      241 gttgatgttt ccggctggca actccctgaa gaaattgatt atgacacatt gtcacgtcac
      301 atctctggcg caattgtacg tgtctatgga ggatcgcaaa ttacagctca taacaacgct
      361 gcttttacaa ctggaattga caaatctttt aaaacgcata tcaaagaatt tcaaaaacga
      421 aatgttcctg tagctgttta cagttacgca cttggtcgta gtactaaaga gatgaaagaa
      481 gaggcccgag ctttctacaa aaatgctgct ccctataatc caacttacta ctggattgat
      541 gtcgaagaag ccactatgaa agatatgaat aaaggcgtaa cagccttccg agaagagctt
      601 aaaaaacttg gtgctgaaaa tgttggtctc tatattggaa cttattttat ggcagaacaa
      661 gatatttcaa caaaaggttt cgatgctgta tggattccga cttatggtag tgattctggc
      721 tattatgagg ctgctcctaa tacaacctta gattacgatt tacaccaata tacctcacaa
      781 ggttatctca gtggttttaa caatgcttta gatttaaatc aaattgccgt aacaaaagac
      841 actaaaaaaa cgtttgagaa gttatttggc aactccaaca attaagaggt tagcatcatg
      901 gctaatcttt ttttatagtt tacatcttac caagaaaaac tctaggtcaa taatttaaaa
      961 ccccttatcc ttgacaagga gattatctca tcatataatg aaagcactat aatatttcac
     1021 aaaggagtgc tttggctgag atcgcaattg cgaaatcctg aggacctgat cttgttagta
     1081 caagcgtagg gattgtgacc aataatcaat gattggaaag cgatactctt tttgtgcaca
     1141 aaaggagttt tttatgtcac caaacaccaa tgtcaaatac cttatcgaag ctgctatttt
     1201 tgctgcactg gcgatggccc tttcatttat tccagatttt gctggctggt ttagtccttc
     1261 ttatggtgct attgcactgg ttattttctc tttgcgccgt gggttaaagt acggcatgtt
     1321 ggcgggtctc atttgggggc tgcttcactt tgtacttgga aaagtttact atctcagcat
     1381 gtcccaagtc tttattgaat atatcttagc ctttacctct atgggattag ccggtagttt
     1441 ctctgatagt ttgattaaaa ctttaagaag acaacaaacc ttttttgccg tttttcttgc
     1501 aataatggct agtttactag ccgttacagt acgttacttg tggcacttcc ttgcaggaat
     1561 cattttctgg ggcagttacg cccctaaagg catgtccgct gtctggtatt cttttagcgt
     1621 taatggtaca gctggtgtcc taactttttt aataacatgt ttagcgttaa tgattgcttt
     1681 accaattcat cctcaattat ttgatcccaa agactaattt ttagtcattt gaatcttaca
     1741 ttaaaaaaat ggtataataa tgttgttata ccatttttta tgtttagata aaggtaagga
     1801 cttgatatgg ctaaaaaaac aaaattcaaa aaaaccttag tagaacaaat cctcgataag
     1861 gctaatattg ctcatcaagg actcaagctt aatgctttag aaggcgactt tccagatgat
     1921 cttcagccat cagatattta caagacactt gctctgactg gtgatcaaac aggtcctcta
     1981 attggcatta ttccattaac agaacactta tcagaaaaac aattggcaaa agtatcaggt
     2041 aacaaaaaag tgtccatggt tcctcaaaaa gacttgcaaa agacaacagg ctatattcac
     2101 ggtgccaata atcctgttgg gattcgtcaa aaacattcat atcctatttt tattgaccaa
     2161 actgcactgg aaaaaggtca aataatcgtt tcagctggtg aagttgggcg ttctataaag
     2221 atttctagcc aggctctagc tgatttcgtt ggcgcaagct ttgctgattt aaagaagaga
     2281 aaatgaacac tactgccaac tttatctggt ttctttttta agtttttcgc cttgattaag
     2341 ctagtctttg tttagtatta ttttgatctc aactgcttta acattgatcc tgttaatctt
     2401 tttcttttgt aaaggcttga tatcacgaca aattcaggtc gatttgtctt aaatctaata
     2461 tgggctttac actttaatca gaacctagct attatacact tatactacgc ttttttactt
     2521 tatcttttca ctataggtat tatttttgct tggtgatgcc taaacttttt ctggttactt
     2581 ttttattttt agcgctcgca tcagttatct ctaaaaggag gcttaaatga aattatattt
     2641 tgtaagacat gggaaaaccc tttggaattt agaaggtcgc tttcaaggcg ctgggggcga
     2701 ttcgccatta cttgaagagg caaaagacga aattcactta cttggcaaag agttagctaa
     2761 ggtggctttt gatgccgttt atactagcga ccttcaaaga gctatggcaa cagcagctat
     2821 tatcttagat gcttttgacc aacagccaaa actttaccac accgaccagc ttcgggagtg
     2881 gagattaggt aaattggaag gggctaaaat agcaacaatg gcagctatat atccccaaca
     2941 aatgctggct tttcgggaga atctagccca gttcaaatca gatcagttcg aggctgaatc
     3001 aatttaccaa acgactcaac gtgtctgcca tcttatccaa tcgtttaaag ataaacatta
     3061 tcaaaacgtt cttattgtag gacacggcgc taacttaaca gcaactattc gctcattatt
     3121 aggttttgaa cctgctcttt tactcgctaa aggtggctta gataatacca gtcttactat
     3181 tcttgaaact aaggattatt taacttatga ctgtttaatt tggaatgata aatcctttct
     3241 agaggtgtaa aaccactaga caataataaa aaaagctaac acattgattt cttactgaca
     3301 agacactcac tttgctagct ttttttatta ttatctagtg ggctattatg gtttcatcta
     3361 tctggcatat tttaagcgca aaactttttc ttgctgcttg gttaaccgaa gcaagataac
     3421 caccttgtca atgctcatgt tactttcaag taaacgctct gctgccatca tcaagccatt
     3481 attgattccc atctgtaaaa ttggattttt cttgtcattg acatcaaaaa taaatttatt
     3541 atcaggtaaa tcaaagagta ctttaacggc accaaagagc tgttcaaagt tatcaatagc
     3601 cacatcataa acaggcttag tgcctggttt tagctgacga cctctatgat taaaccacat
     3661 ggaatgccaa ccaccattaa aggcacccat gatatcattg tcataagagt ccccaacata
     3721 tagtgtggtt tgtgggttca tgtcaaattg ttctgctgcc aggttaaaaa tttctttttc
     3781 tggtttttga aagcctgttg cttggctcac aatgacacgc ttaggatcaa tataatcata
     3841 aagccctaat ttacgaactt ttttcagctg atgttctgtg ggcccattgg taataatccc
     3901 cataggaaca ttctttgatt ttaaaaagtc aagggtcata cgcatctcat ctaacatggt
     3961 aatgttctca agttcgtgct catagacttc ttgaaaatgt actcccgcag cttcgtcgat
     4021 ttctcgatag ccaaactcaa gcagtgtttc cttgcaacgc cagaatcgga aatattctgt
     4081 ggtccactcc cctgccatta ctcttgggaa tccaacatct gaatagtgac gaaagcgaat
     4141 ataagcttga ttcattactg acatatcaaa atctgggaag cacttttcca tggcgattcg
     4201 ataaggcgct tgctggtcgt aaattgtgtc atccacatca aaaacaatag cagtaatcat
     4261 gagtctcttt tcttttctag tttaatatcc caatcattat actattttcc attaggcttt
     4321 tcaatttcat tttcaaaaaa atgccccgct gcagtcgcat tttccaacga aatataggtg
     4381 tattctagcc ccttttatga tacaatggaa aggaaatcgt attggaggaa aatatgtcta
     4441 atcaacacat cgaagaatta aatgaccaac agattgtccg tcgtgaaaaa atgacggcgc
     4501 tggctgaaca aggaattgat cctttcggta aacgctttga ccgcacagcc aattctgctg
     4561 aattaaaaga aaagtatgct gataaaacaa aagaagaatt acatgagtta aacgaaacag
     4621 ctattgttgc tggccgcttg atgacaaaac gtggcaaagg taaggttggt tttgctcatc
     4681 ttcaagaccg tgaaggccaa atccaacttt atgttcgcaa ggactctgtt ggcgaagaca
     4741 actatgaaat cttcaaaaaa gccgatttag gcgactttat cggcgttgag ggcgaagtca
     4801 tgcgtacaga catgggcgag ttgtcaatca aggcaaccaa gttgactcac ctttctaaat
     4861 cacttcgtcc tcttcctgaa aaattccacg gattaactga tattgaaacc atttaccgta
     4921 agcgtcacct tgatttaatt tcaaatcgtg aaagctttga ccgcttcgtg acccgttcca
     4981 aaatgatttc agaaatccgt cgttatttag acggtcttga tttcctagaa gtagaaacgc
     5041 ctgtgcttca taacgaggct ggtggtgctg ctgctcgtcc tttcgttact caccataatg
     5101 ctcaaaacat tgatatggtt cttcgtatcg caacagaact tcatttgaaa cgtttgattg
     5161 ttggtggtat ggaacgtgtc tatgaaattg gtcgtatctt ccgtaacgag ggaatggatg
     5221 ctactcataa tcctgagttc acttctattg aggtttacca agcctatgca gactatcttg
     5281 atatcatgaa cctgacagaa ggcattatcc aacatgctgc taaagctgtt aaaggtgatg
     5341 gccctattga ttatcaagga actgaaatcc gtatcaatga acctttcaaa cgtgtccaca
     5401 tggtagatgc tatcaaggaa gtgacaggtg ttgatttctg gcctgaaatg actgtcgaag
     5461 aagctattgc acttgccaaa gaaaaacagg tcccactcga aaaacacttc acaagtgtcg
     5521 gacatatcat caatgctttc ttcgaagaat tcgttgaaga aaccttggtc caaccaacct
     5581 ttgtgtttgg tcatcctgtt gaagtatcgc ctttggctaa gaagaatcca gaagataccc
     5641 gcttcaccga ccgcttcgaa cttttcatca tgaccaaaga atacgccaat gctttcacag
     5701 agttgaatga tcccattgac caattatctc gttttgaagc acaagcacaa gccaaagaac
     5761 ttggtgatga cgaagcaact ggcattgact acgacttcgt tgaagctctt gaatatggta
     5821 tgccgccaac cggtggactc ggaatcggga ttgatcgtct ttgcatgtta ttaacaaaca
     5881 caacaacaat cagagatgtg cttttattcc caacgatgaa accttaactt ttttaagatt
     5941 tgtattcata tcttaaaacc gataagtggc aatataacat aacgttttat tatctctaac
     6001 cagtcttcat taccatgaag actggtttat taacagtagc ttatagaaag tgacttaaca
     6061 agatgaccaa agaatatatc tcctacatta gtgctgctat cttcttgctt tatggccttt
     6121 tagctgccaa atggattttt cttgggctag gcttaatttt ggtacttgtc ggccttgctg
     6181 atcgcctcaa ttcaaaaaaa taatccttct attcgcccct gtccttttaa cctagcgatg
     6241 tcatcaccag gttaaaagga caagggcgaa cgagggatta tgtttcgatt aaggtaacat
     6301 tacttacgaa aagcgtctaa gatggtttta aattcttcat tagtaagagt aatgcctttc
     6361 cccattttcg tatggtctgg actccacgtg cggatatccc atttcgcttc tgcgccatta
     6421 aagctgacgc ggttcaattc tttcgtccaa cctttatcat tttctgataa ggtgagtaaa
     6481 tgttcctcaa tgttgaatgt aaattctgcc attagttttc ctcctcatct atactattcg
     6541 aaattaaagt ggaaaaaatc atcttttttt cacttttttt atgactcatt ctcctattat
     6601 tctatcaagt tttgagaaaa taagctattg tgatatccaa ttttcaaaaa aaaaaaaaac
     6661 aggctctctt ttcccaacta tggtcgtata ccatttggaa aaagagggcc tctaatattt
     6721 cttgttacta tgttctaaca gttttactgg tgccatcttt ttgatagagg ttgacaaggc
     6781 cttccttgca agccctaatc atatcccctg gcttaacttc tctcggtaaa gagatggtta
     6841 agagttccat tggatttggg gcacggtcaa tcttttggcc atccgcatca tgtaagtcct
     6901 taacaaccgt ttcaaaatga cggaaacctg gtccataaca ttcaatccga tcgccttcca
     6961 tgatgacatt acgctgacga atggtcgctg tcatgctagc tgagtcaaag gcaactactt
     7021 ctccgacaaa tttatattgt ggaattttgc ggcgagcacc aaataattgt tcattttcag
     7081 ttgggatacc ataataaaaa cctgtagcca actcgcgctg ggcaaccttc cacaactcgt
     7141 caatcaattc ctctttgata gcataaaaag cttctgggct ttccatgtaa gcatctacag
     7201 ccgccttgta acagttggtt acggttgaga cgtagtggat agatttcatt cggccttcaa
     7261 tttttaagct atcaacccca ttttcaatca ggtcaggaat atggtcaatc atacacatgt
     7321 caacagagga catagaatag tcttctggaa tttccccttt taaggagcgg cgctctcctc
     7381 caaatggcat gtcatacaaa tcatacttcc agcggcaaga ctgtgagcag ccgcccctgt
     7441 tggcatcacg gtgactcatg tggtttgaca aaacacagcg gcctgaataa gaaatacaca
     7501 tggctccatg gacaaaggct tcaatttcca catctgtccg cttacggatt tctgctaact
     7561 cggccatatt aacctcacga gctaaaacaa ctcgggtcaa acccatggct ttccaaaatt
     7621 caaaggtctc gtaattggta gatgaagctt gcgttgacaa atgaatttcc aaacctgggg
     7681 cttctgttga acaaataata atcaaggctg gatctgaaac aatgaccgca tcaagcccca
     7741 tatcacgcag ttgacgaaac cactcgcccg caccaatttc gttcccttcg tgggtaacca
     7801 tgttggcagc aacatagacc ttagctccac gcgcatgtgc ataatcaatg ccttcttgca
     7861 attcttccat agagaagtta ccagcgcggc ttcttagacc ataggcctgt cctccaacaa
     7921 aaacagcatc tgcgccatag tcaatcgcaa cttttaattt ttcaagtgtt ccagcaggtg
     7981 ataagacctc gggacgtttt ttcatatgtg acatgtgata ttctttctat tttcaagata
     8041 ggtagattac ctgtgtaatg gtgtcataac cctgtcttgc cctgaaatag taaactcact
     8101 caagacaagg attacttatt taacggtttt tggatcgaac tcataaaagc cagtatctaa
     8161 tccacggcca gctggatgat gggcatgcac tgcttgatca agcttttcag cttcttcttg
     8221 ggtaaattgc ccagcttcta ataaggtttt agcctgaata aacaatttag taatagcgac
     8281 aaaatcatca cctggacagt aaatgccatc tagcttccaa tgagttaagc catgcgcata
     8341 cagctcaccc aatttagaca tcatgtcgat atcgttatta atgaaaatat gagtgccatg
     8401 attgtcttca taaatggagt aatggctgct agcatctcct ggttctgcca agaaaagtcc
     8461 tcgttcacga ctaacctcat catcaatctt tgtaaaatgg taataatttt ctagcaaggg
     8521 gcgtttcgag tgatgaatca ccgaggcgcc gtaaactagc acctctgctg gaaattccaa
     8581 attttcagct aaggtgaata actctgctga tgggatttcc cttgctaaaa cactttcaac
     8641 agcaccatgt tgcccccaga aatttacctg acggctagaa gtcacaaaaa cagaggtatc
     8701 ataaatcagt ttaaagttat agccatccct tttattgaca taaaacacac ctgcatcacc
     8761 aaccacaaga taatcaacag caatctctat cattaagtct aaaaagggtt tgatctgatc
     8821 catcatatct tgatgcatca aagcattgca agctactgtc aactctttgc ctgcatcatg
     8881 cactagtttg gcaatttgtc tcaattcatc ataagaaaaa ttatggggaa ggcgtaaccc
     8941 gtaattggct tcaccaacat aaatacgatc aacaccagca gctaataaag ctttgacttg
     9001 ctcaatgctt tcagctgtgg ctgtaataat tattttttcc atataagctt tattatacca
     9061 aaaattttaa gaccttaagc atatttcttg caaaagccca aacgttaagc tagtcccctt
     9121 actgttatag catgataagt cacttgtcat tagtaaatct tttcaaatct tttaaaaaaa
     9181 gatgacaaga agagactttt atgatagaat tacttagtat gttaaggaaa ggagaggact
     9241 atgcctgcac cgttaaagaa gcataattcg gttgaacagg atgattctta ccaagaatct
     9301 caaagtaaat ttcgagattt tcaagagttc aatggccaaa gtgcaaagct aaaagaatta
     9361 gtcttctttg ctcgtatcgc tagtttcagc cttattaccg ttttatttgc atttgtcctg
     9421 ctcgtaatga atttagcgcc aatgtttgct ttcttatttg caagcatcat cagcttaggc
     9481 attacttcct tattcgccaa ggttatcaaa tcattactga tgtaacttcc agagtccgtt
     9541 ttacgtgact cttttttttg tgtcaaaaac aaaaaaagat tgaagccaca atccccccct
     9601 tcttcagtta gcctaaaact aaccatcggg actagtgatc agatccttca atcttttctt
     9661 cttctatgtt ttaggtcgtt ttagttagtg tttatctagt gtatcactaa caggctcatc
     9721 ttgttcagta tagtcaatga tgatgtcttc ttcatttaaa tcaatgataa catcatcttg
     9781 cttttctttc gcttcaactt cggcaagggt ttcttttgtt ttttgcacga aagctgttgt
     9841 tttatctttg agcaagtcta acatgtcctc tttagttaag tcaccagaag ccaacttatc
     9901 tttcacatca tagaaagtgt cacgtgctaa atggctgtat tcactacctt tttctttagc
     9961 aaattggtgg taatcatctg gactttcttt gtaggcttga taagcttttt cagcacgatt
    10021 tttcaaagcc ttgccttttt ctgttgataa gaagtaagct gccgctactc ctgaaacagc
    10081 tccaatcact aagtttttga aacttttatt catgcttttc tccttttttt ccaaatactt
    10141 ttgaggctac tttaccaact aatgctaatt ttccagcttt tgaaacatta cctgtagcat
    10201 tagttgcttt ttgcccaaaa tgccttgctt gactatttaa atctgaaaga ctttctgaca
    10261 aatcagcaat agcaacaaac agtggatcga tggttgctac tttaccatta acatcttcaa
    10321 caaggatatt agcttttgct aaaatatcat tagtttgatg aagtgtcaca ttaacatcac
    10381 ttgtcaatac agaaatcgtt tttttggctt catcaattgt ttctgagact ttctttaata
    10441 caataatcaa gaaaatcacc aaagcaacaa atgcgagtgc aataatcatc agtgagattc
    10501 caactaagtc cataagtttc tcctttttct atccttattc ttgataataa gaaattcctt
    10561 tttgacggcg tctttttatg acaaagatta agccaataat aattaataag gcagacacat
    10621 attgggagat gcgaatacct aaaaacataa gactatctgt tcgcatacct tcaatgacta
    10681 gtcttccact accataccaa attaagtaaa atgcaaagat ttctccatct aatagacttt
    10741 ttggcttacg acgccacatc ataataatga caaatcccaa aagattccaa agtgattcat
    10801 agagaaaggt cggaatgcgg taacttccct ctataaacat ttgcttttga ataaagcttg
    10861 gtaagtaatt taactggctc acagctttac cataggcttc ttggttgata aaatttcccc
    10921 agcgaccgat agcttgagcg accatgacac ttggtgcggc aatatctaaa aaatgaatgg
    10981 gattgagcac cttgttataa cagtaagcca aaagtacaag agctcctgta atgagaccgc
    11041 cataaatagc aataccgcca ttccaaatgg ctatgatttc atctaaatgc ttgacataat
    11101 aagaccattc aaaaataaca tagtaaattc ttgcaccaac aatagcaagt ggaaaagcaa
    11161 tcagaataaa atcgaaaata gcgtcagatg atattccttt tttgggtgct tcttttgatg
    11221 ctaagtaaac cgccaaaaca agtcctgaca agatacatag cgcataccag tggatggcta
    11281 gtggaccaca ttttaaggca atgggattga tcattgactc acctcatttt tggtgattag
    11341 ttgagttaaa cgatcttcaa aagtttttgt agcatcaaag cccatttctt tagcccgatg
    11401 attcatcgca gcggcttcaa taaccactga aacatttcga cctgttttta ccggaatacg
    11461 aatacgaggg attctcactc ctgagaaagt tatctcctca ttaccgtttc cgagacgatc
    11521 aaaaacttta ccagcctcaa agttttcaag gtaaatggcc aattgaactt gtgatgagtc
    11581 cttcacagca ctagctccat aaagcgacat cacatcaata atacctaccc cacgaatttc
    11641 aagcaagtgg cgcaagatct ctgctggctc tccccaaagg gtttcttcat ctttggcata
    11701 gacatccaca cggtcatctg caaccaaacg atgccctcgc ttaactaatt ctaaaccagt
    11761 ctcactttta ccaattccag aatccccttg gataagaacc cccataccat aaatatccat
    11821 taaaacgccg tgaacactag tacgttcagc taaagaagcg tcaagaaaat aagacatttc
    11881 tccagctaag cggctagttg aaacacggct acttaaaagt gaaatacctt cttcttttgc
    11941 agcttgaacc atttcttttg gaatggctaa atttctagag acaaccactg caggagtgtc
    12001 ctttttgaac atctctttta aaacagaata acggttgtga gaagtcatct gtgtaagata
    12061 agaccactct ttcattccaa atagctgcaa cctttctgga gcataataat caaaataacc
    12121 agtcatttca agacctggac gtgaaatatc tgatgttgtt atctcttttg acaacaagtt
    12181 atctgtagca taaaccacat caagtttaac tttttgcact agcatcttta cagtaacggt
    12241 cataatttct cctttttcaa tattataaca caaatcgacc gttagaccct tgcaaatact
    12301 cattttttca ctactaaatg agaacaacga caccaaaaat ggtcttagct ctattaaaaa
    12361 ctaggtttta tcatttagag atatgagtca aaaataagac aatggcaaac aaatacttta
    12421 ctagtatcat tgagcaatta gtgcttatta tggtgtttaa aaaggtatca tcttactaag
    12481 tcaaaaagca aaaagcctca tcatagctga taagacccct cgcttactta catatcaaaa
    12541 cattttatta tcatcagtta atcacatggt tgctcttacg tgatgaatta tcattttaac
    12601 tgattgttta ccagaaccag ccatcttctt cttcatttaa gacatcagcg tctttacgac
    12661 gacgaggacc tcggccataa cgttcttcaa gcaagtcttc cttgtaaggt aagaagatgg
    12721 ccaacaaaat ataaagcaaa actccaaaac cagttccgta aataagaaga gccgctaaga
    12781 cacgcgctag agcaagatcc caaccatact tatctgctaa accagcaata acacctgcaa
    12841 ccaagcgatt ttttcgctgt ttatagaatt tagtttccac gtttctttac ctcctcattc
    12901 tatgatacta gtttacattg tttttcgcaa aataaaacca gtcttacgac tgattttttt
    12961 ccataagttt cccgtggcaa tttccacaaa catatttagc cagattgata cgcctttttc
    13021 gttgataaac ctgcccacag gtttggcaac tataatgatg attagtcttt gatttagagc
    13081 tagttgggac ataacgaagc ccgtttactt gagctagcag atctttgaaa tccctatctt
    13141 tatgatggta ccctcttcct tcaaaataaa gatggtaatg gcagagctca tggcgaacaa
    13201 ttttccgaaa aataagctca ccgtgctcct caagcatcct agggttaaaa tccaaatgac
    13261 catcttttgg aaaaaagcga ccgccagttg tttttaggcg cttgttccaa taagccttgt
    13321 ggtgaaatgg tttgccaaaa tcagccaggg aaacttcttg gacataatta gtgagtgtca
    13381 cgcggtggca ataggctgag attgaccttg tggcggtcca agtcaatctt agagacccaa
    13441 acagttacca agtcacccac tgaaacaact tgactagggt ggttgacaaa ggttttgctc
    13501 atttcagaaa tgtgaataag cccatcttca tgaacaccaa tgtctacaaa agcaccaaag
    13561 tcaacgacgt ttctcacagt tccttcaagc ttctggccaa tttccaaatc tttcaaatca
    13621 aggatatctt gacgtaagat tggtgcttca aagtcatcac gaaggtcacg acctggttta
    13681 aggagatcag caatgatatc tttaagggtt tcctgcccaa tagccaatgt ttctgccatt
    13741 tggggaactt gaactgctgc taaagttgcc ttggcagcgt cgtccaagtc ctgaatacca
    13801 agtaccttaa agagttcttt gactgctggg taggactcgg ggtgaactcc tgtgttatct
    13861 aaaatatttt tggctcctgg aattctgaga aaacctgctg cttgctcaaa cgccttagct
    13921 cctaaacgcg gcactttttt gatgtcagcg cgtgatgtca atgctccatt ttcttcgcgg
    13981 tatttgacaa tattttctga aatcgttttg ttcaaacctg acacatgtgc taataaagat
    14041 ggactagccg tgttcacatt aacaccgact tggttaacca cagtatccac aacaaaacca
    14101 agattctcac tcaacttttt ctgactcaca tcgtgctggt actgaccgac gccaatagat
    14161 ttaggatcaa ttttaaccaa ttctgctagt gggtcttgca agcgacgagc aatagaaata
    14221 gcagagcgct tctccactgt gaggtctgga aactcatgac gggctaactc tgacgctgaa
    14281 taaaccgaag caccgctttc gttaacgatg acataggacg tatttgggaa atctttcaaa
    14341 acgtctgcta caaaggcttc actttcccga ctggctgttc cgtttccaat ggcaataata
    14401 tcaatctggt aagtctcaat gagctgagtc agtgtttctt tagctgcttg aatcttcgtt
    14461 tggctagctg gtgctacggg ataaataacc tgagtagtca acatttttcc agtctgatca
    14521 acgatagcca attttgcacc tgttcgaaag gcagggtcaa acccaagaac cattttccct
    14581 tttaaaggag acactaacaa aagatgacgg agattttcag aaaagaggtg gatagcccca
    14641 tcttctgcgg catcactgag ttctgaacga acccgtcttt ccatagctgg aacaattttc
    14701 tttttgatgg tctgattgat gacctcttca atataagggt tggtttcttt gaaccgcaca
    14761 ctgaaaaagc gttgcatttt ctccaggtta tgctcaaaag acacttttaa gatgcctaac
    14821 ttttcgccac ggttaagggc taaagtacga tatccttgca tgttagacac ttggtcagaa
    14881 aaatcgtaat agatttggaa aacctttttc tcatccaact gctcatcttt aagcgttgat
    14941 actaaacggc tgtactgcca aatctcattg taggtccaag agcgtaattt agcatcttct
    15001 gacatggcct ccacaagaat gtccacagct cctgctagag cttcttgtgg gctggcaaat
    15061 ccttctgtga caaagggttc tgctgctgtt tcaaggtttt gcgcattttg taaaatcaaa
    15121 cgtgctaatg ggaacaagcc tgcttcacgc gcaatcgtcg cttttgtacg gcgtttttcc
    15181 ttataaggca ggtacaactc ttccaaatca gcgagttttt cggttgcttc aatactcgtt
    15241 cgtaactgat ctgtcagttt tccctgttcc tcaattttag cgagaatggt tgctttgcgc
    15301 tcattcaggg tagtgagaga cttatccata tcaataatgg acttaatcac cacttcatct
    15361 agatttcctg ttacttcttt acggtaacgt gcaataaagg gaatggtatt gccttgtgct
    15421 gttagggcaa gcacctgttc aatttgatgg agactaactg acaaagcctc tgcaatgttg
    15481 tgattattgt tattttccat aatattgcta ttataacaca aaccatgtta atgcaaaaaa
    15541 tgatagcgaa acattgtcct aatataaagt tgcgtaaaga atcaaaaaaa gctactttta
    15601 gtaaataact gacgaaaccc taaataacaa tacctaattt taaaaaagaa agaagtaaac
    15661 agcacttccg ctttggcatt gttctcaaat gatagtataa tagacttatc ttgaatgaaa
    15721 gaaggtcata gatatggcaa ttacccataa aaaaaatgat gaacttgaaa aaatgctagc
    15781 aggctttgca tcaatcccta gttttgataa gcctttggaa gtcaatactg atggtaaact
    15841 agcgaccaaa gaaaaaacga cagaacaaac aaaaccaagc aaaccatagc tagtttgtct
    15901 cattctctta acatatcgtc cattcgttta aactgagttg agagattttt agcgatcagg
    15961 ctaacaaaac gtatcagtcg aggctgaaca ttcgtgtttc agcctcctct tttatcatct
    16021 agaaaggata tggtgaatgt cactcttttc caaccttcct cccagtgtct tgcaatggtt
    16081 tgcgattttt atgtccatta tcattgaagc tcttcctttt gtgctcctag gaactatttt
    16141 atctggttgt attgaagtct ttgtaacgcc agaattggtt caaaaatacc tacctaagca
    16201 aaaatgcttg cgcattctgt ttggaacctt tgttggattt gtttttcctt cttgtgaatg
    16261 cgggattatt cccatcatca accgcttttt ggaaaaaaag gtgcctagtt atacggccgt
    16321 cccttttctg gcaacagcgc ctattattaa tcctattgtt ttgttcgcta cttattctgc
    16381 ttttgggaat tctttgcgct ttctgatact tcgcttggtc ggtgctgcac tagtagccat
    16441 tacacttggg gtaatgttag cttttattgt agatgataac attctcaagg ataatgctca
    16501 acccgtgcat tttcatgatt attctcatga aagcttgcca aaacgcattt atctagcact
    16561 ggtacatgct attgatgaat tcttcgacac cggtaggtat ctcgtatttg gaaccttgat
    16621 tgcctctgcc atgcaaatat atgtcccaac tcgtgtgttg acaacgattg gacacaatcc
    16681 cttgacagcc attttgatca tgatgctgat ggctttcatt ctatcattat gtagtgaagc
    16741 cgatgccttt atcggagctt ccttattatc cacctttgga gtggctccag ttcttgcctt
    16801 tcttttaatt ggtcctatgg tggatattaa aaaccttatg atgatggtaa agacttttaa
    16861 aggacgcttc attgtccaat ttatcggcgt atccgttctt atgattgccg tttattgttt
    16921 acttgtgggg gtgctctaat gattcgcttt ttaattttag ctggctattt tgaactaacc
    16981 atgtatctcc agctgtctgg caaactggat cagtacatca atgttcggta ttcttacctt
    17041 gcttatattt ccatggtttt atctttcatt ttagctcttg ttcaactcta tacctggatg
    17101 aaaaacatta aggtacatag ccatttaacc ggaaaaatcg caaggctgac cagtcctttt
    17161 atcttggtct ttcctgtttt gattggtctc cttgtgccga ccgtaacctt agattccacc
    17221 acggtttctg ccaagggtta cacttttcct ttggctgcgg gagcctctaa aacgggagtc
    17281 agtgatgacg ggaccaccat tcaatacctc aaacctgata ccagccttta ctttacaaag
    17341 tctgcctacc aaaaggagat gcgtcaagaa ctccataaat ataaaggcaa gaaaccggtc
    17401 accattacca ctgaaaatta tatggaagtg atggaattga tctatctcta cccagatgag
    17461 tttcttgatc gtgacatcca atacactggc tttgtttata acgaaccggg ccatgacaac
    17521 taccaattcc ttttccgctt cggcattatt cactgtattg ccgactcagg agtgtacgga
    17581 cttttgacca caggtaatca aaccagctac cctaacaaca catggctaac agttaaaggt
    17641 cgccttcata tggaatatga taaaaactta gaacaacatc tacctgtcct tcaacttact
    17701 gaggttcatc aaaccaaaga gcctaataat ccttatgttt accgcgtttt ttaaagacta
    17761 ataaaaaagc acttgttttt tatcaaaaca aatgcttttt gagttttatc agttagcata
    17821 ggaacagagt caccatctta aacaagacct atttaggaaa aattatcctc aaggtaacgt
    17881 ccattggttt gaatgacttg tcggtaccaa tcaaacgatt ttttcttata gcgtgtgagt
    17941 tgaccagtac catcatcatt tcggtcaaca taaataaaac cgtaacgctt acttaattgg
    18001 gctgttgaca tagagacaca atcaatacag ccccaagcag tgtaacccat gacctcaacg
    18061 ccatcttcaa tcgctttggc aacttgcacg agatgctgac tcatgtagtc aatgcgataa
    18121 tcatcgtgaa ccgtcatgct gccatctgcg gtttgcacca gctgatcctt tgcacctaag
    18181 ccattttcaa caataaacaa aggaatctgg tagcggtcat agtactgatt caagaccaat
    18241 cgcagaccaa ttgggtctat ctgccaaccc cactccgaag attctaaata tggattggac
    18301 aaaccaccta gaatatttcc ctgaccagat gtgtaagctt caggattgtg ggcttgtgtg
    18361 acactcatgt aatagctaaa ggatagaaaa tcaaccgtat gttccgctaa taattccttg
    18421 tctccgtctt caaaaacaat ctcaatccca ttttctttaa aatacgactt aatatatgaa
    18481 ggatacttgc ctctcacatg aatatctgaa aataagagat taagattttc aaatgcatgc
    18541 gcagcaagta tatcacgagg atcagaggtc attgggtaag caggcatcgc caaaatcata
    18601 cacccaactt taacatcagg attgatttca tgcgctagct ttgtgacact agcagatgct
    18661 accaattcat gatgaatagc ttggtataaa tcttgcaggc tcagcttttc tttttctgtc
    18721 atgatgccac cactggtaaa gggcatgtgt aaaatcgagt tgacttcgtt gaaggtcaac
    18781 cagtatttga ctttgtcctt atagcgttcc atgactgtct gagcaaagcg ctcaaaaaag
    18841 ccaatcagac gtctatctgt ccaaccatta taggctttgg ccaaatgcag aggtgtctca
    18901 taatgcgaca aggtcactaa gggttcgata ccatagttca acaattcatc gaataagtca
    18961 tcatagaatt gcaaacctgc ttcattgggc tggtcatcgt caccatttgg aaaaatacga
    19021 gaccaagcaa tagatgttcg aaagacttta aagtccatct cggcaaaaag agcaatgtct
    19081 tctttgtagc ggtggtaaaa atcaatcgcc tctaaagtta aattatcgct ggtaggacta
    19141 tctgtccatt ctcctagtcc accatttggt agaacatctt gtacggacaa accttttgtg
    19201 tctgcctcaa atgctccttc cacttggttg gcagctactg cgccgcccca taagaaatct
    19261 tttggaaaaa ttcccattct ccctctcctt ttaggttata aaatgactaa caaagcgtcc
    19321 tctttagtca ccattttttt gtcatcttga ataataatct ccaactgctc agccgaattg
    19381 gtcacgacca caggactgat gagtgaatag ccttttgaag taatgaaatc aatatccatt
    19441 tttagaagtg cctggcctcg tttcacgaca tcgccaacag acactaactg ctcgaaacct
    19501 tgccctttta actcaaccgt atcaataccg acatgaatca agacttccac accacgtgtg
    19561 ttttttaagg caacagcatg cttggttgga aagaaagtga tgacttcacc atcaaagggg
    19621 gcatagacca caccttcgct tggtaatatg gcaaatcctt ctcctaataa cttggaagaa
    19681 aaggtttcat caggaacttc tgatagaaaa agcatctcac ctgtcattgg agcatacaac
    19741 gtttgtttgg ttgacaagcc gctcttaact tgtgacatat ctgctgcaac actcacttgc
    19801 tttggggact cttcatcaat tcccatgaac caagtcaaac tgaaggctaa cacaatcgtc
    19861 gcagctccgg caatcaaagc atttataaaa ttagctccgc ctgatgggtt gatgtactgt
    19921 ggtaaaccaa taatagaagg tacgacaaag gtataagaag caatattgac aaaaccaata
    19981 aaggctccaa ccagaccacc tgaaatcatg gctgcataga gtggcttttt aaatttcagt
    20041 gtgaccccat aaagtgccgg ttccgtgata cctgccagca aagctgaaat ccccgcggca
    20101 agggcaactt gacgtgtttg tttctgcttt gttttaaagg caacagccaa agatgcggct
    20161 ccttgagcca aattagaagc caacattgct ggtaaaatca agacatctgg tgttgcgacc
    20221 gaagcggcca aaaagattgg tgcaaaagcc cagtgcatcc ccgtcatgac aacaagcggc
    20281 ataatcgctc ccaaaatggc aagtgctagc cacggagcca catgataaat agcgagcatg
    20341 gtattagata acccttgacc aataatcaca ccaagaggac ctacaaccac taaagccaaa
    20401 aagccagaaa tcaaaatgat taaggttggc tgtaagaagc ttttcatcac gcttggcacc
    20461 aagcgattga cccatttttc aatgtattgc atcaagtaaa ccatcatcaa aatgggaata
    20521 acagatgagc catagctagc aggagtgact ggtgctccaa ataaagtcaa tggtttacct
    20581 tctgctacca ttgcaacaaa ggcaggatgc aataaaatcc ctgcgatcgt cgctgctaaa
    20641 acaggtgtca ccttaaagcg cgccgctgct gaataggcaa tcatgacagg cataaaataa
    20701 taagccgcat cgccaaagaa gttaataaag gcaacggttt gagagtccgc actcgctaag
    20761 ccaagcatag gaagcaagat tcccaccact ttgagcattc caccaccaag aagggctgga
    20821 atcagcggtg taatcacgcc agcaatggtt tcaatcaagc gctcaaggat acttcctttc
    20881 gcttttgaag aatgatcttc atcttgattg tcaaaatgcc ctaggcttaa aaaggcttgg
    20941 taataattat tgacatcatt gcccaagata atttggtact ggccgttttt gcgcatcacg
    21001 ccaatcacat ttgaaatggc tttgacttgc tgatctttaa ctttctcatc atttttcagt
    21061 actaagcgta gacgtgttac acagtgtgtc acacgttgaa tatttgtttt gccaccaacc
    21121 gccgctaaaa tagccttggc agtttcttga tacgtcatat taagtctcct ttaaatgttt
    21181 cactaaacgt ttaacatgaa ccgtcaaata caaaagttcg cttgaattaa ggtggtagtt
    21241 gtaccgcttt tttatctcct cactaatatc taagacgcac cggtattctt tagggtattt
    21301 cgttttcacc actaaaagca gttccgcatc atcatcatcg taagacatct ctgataacac
    21361 acgttgagca aataatttaa tatgggtcat aaagcggtaa taatcgatgg atgtgtcatc
    21421 caattctgtt ttgaagtctg ttttgatttt ttgctcaata taagaaacaa tctccgtcag
    21481 gcgatgtggc tccttaaaag gagtgtccaa actagcattg acaaaatgca tggcaataaa
    21541 agctgcttca tcaatagcta gagtgactcc tagattacgc tcaattagct ctaaggcttt
    21601 tacccctaga ctatattcat caggataata gcgttgaatt tcccaccgta gtgggttatg
    21661 aatcaacatt ccttgctcat gcctttcaat agcagagtga atatgatccg ttaaattaat
    21721 atagagaatt tcatctaagg ttttgcctaa tttaatttta cctagattga taattctctc
    21781 tgagcaagca acaacttctt gtggcaccgt aataaataac tctgtgaagc gattcatatt
    21841 gtcactattt tttaaaacaa atgatgtttc gatagcatta agctcaatac tatctccaac
    21901 tttttttcca aaggcaatcc cttttcccat caataaaata tctaatcctt gatggttggt
    21961 cgaaatcgcc gcattatggt ttaaaacgcg tttaatcaac ataaaagtcc tagtccttta
    22021 gtatcacaat aaaaaatgac ctatccaaaa gctcgctaaa aagcactttt cgaataggtc
    22081 cctgctcaca aaactgtgat aacatccttt aatattctga ttgttatgtc tctagcctgc
    22141 ttacttgaag taatcacatc tgagaacgct ttaatgttat catctcttta agattctgtc
    22201 aagcgttttc aaaaaattct ctgaaagagc aactattgtt attcgtatct attgatttag
    22261 caaaaaagag tttctgacaa ttttaagatt atatgataca ataacaatcg attagataaa
    22321 ggagttatca tgtcacatca tcaacaaacc gtttcaaaac aaacaattat ggcgattatc
    22381 gccatagcac tcattggttt ttcaggaatt ttgtctgaaa ccagtatgaa tgtcaccttc
    22441 ccgacactga tgtcagtcta tcagttaccc ttaaatagct tgcaatggat gacgaccatt
    22501 tatttactag cagtggcgat tatgatgacc acttcggcta cactgaaaaa aaatgtgcgg
    22561 gaaagacccc tcttttttat ggccacaggt ctttttacat ttggcaccat cttggccgtt
    22621 ctgacccagt cctttgcgat catgttgcta gcccgcattt ttcaaggcat tggtactggt
    22681 ctggtaatgc ctcagatgtt taatattatt ttagagcgtg tcccaatgca taaggtaggt
    22741 ctatttatgg gatttgctgg tcttattatt agcttagcac ctgcttttgg gcctacttat
    22801 gggggcttta tgattagcca ttttagttgg caatggattt ttatctgtat actccctgta
    22861 ccactgattg caggtattct agcttattat tacctcgaag attctccagt aagcgaaaaa
    22921 gtaccctttg actggttggc atttattgca ctatcgatta gcttaacttc tgccttatta
    22981 gctattacta gtctagaaaa cggaagtgtt aatttgtatt acttagggct ttttattctc
    23041 agctttgtcc tcttcctcta caagaatctc acagctaagc aaccctttct tgatattcgc
    23101 attctcaaaa ttccctctct aacctttggc ctgattccct tttttgtctt ccagctgatt
    23161 aatttaggca taaattttct aacgccaaac tttattgtca tggaaaagat tgctaatagt
    23221 tctcaagctg gtatggtgtt actacctggt accttactcg gagctctact agcacctgct
    23281 tttggtaaac tttatgatca aaaaggagca aaactttcgc tttatttagg aaatgcctta
    23341 tttagtttat cattgattat tatgacactt caaacaagac attttatgct tttaccattc
    23401 actcttttat atattttatt cacgtttggg cgtaacatgg gctttaataa tagcttagcc
    23461 acggctattc gagaattgcc tgccgagaaa aatgccgatg ccacggccat ttttcagatg
    23521 atgcagcaat ttgctggcgc tctaggaact gctatggcat cactgatagc aaatagtcaa
    23581 ccagaattca caagcggtgt ccagtctgtc taccttctct ttactatttt tgctctactt
    23641 gattttatct ttttctttgc tatgttttac catttaggga aaaaaggact tgcctaagct
    23701 gacttactta aaaaccagcc tcgttctgtt gaacaagact ggtttttttc ttataaaata
    23761 ccttctaaaa gacctttcat aaagtcttct gagtggaatt ctccaatatc atccactttt
    23821 tcaccaaatc cgatgaactt gactggaatg tctaattctt gacggatggc taggactaca
    23881 ccacctttgg ccgtgccatc aattttcgtt aaaatcaaac ctgttaatgg tgttattttt
    23941 gaaaattcct tggcttggct aagcgcattt tgacctgttg acgcatctaa ggccaaaagc
    24001 gtttcatgag gggcatctgg tagcacgcgc ttaatgatac gacccatttt ttccagctct
    24061 gccatgagat tttctttgtt ttgcaggcga ccagccgtgt caatcaggag aatatctaca
    24121 ccttttgcca cagctttttc catcccgtca aaaacaacag acgctggatc agccttttca
    24181 gggccagtaa tgactggcac atctactcgg cgtccccatt cgactagctg agccacagcc
    24241 cctgctcgga aggtatctgc tgccaccaac atgacttttt taccttcttg tttgtaacgg
    24301 taagccagct tcccgattga agtcgtttta ccaaccccgt taacacctac aaaaagcata
    24361 accgtcaagc catcttgata attaatggct tcattataac gcccgtcttt ttcatagata
    24421 tctaccaatt tttccacaat cacacgtttc aaggcatctg gttttttcgc attttccagt
    24481 ttggcttcat aacgcaactc ctctgtcaaa gttgtcgcca catgtacacc gacatctgat
    24541 aagatcagca tttcttccaa gtcttcaaag aattcttcat ccactcgcct gaaattggca
    24601 aagaaactgt tcagacgagc tgagaagcca gtccgcgttt ttttcaagct acgcttgtat
    24661 ttttcttgat ctgtctcagg aatggtatcg tgggttgcct tgttttgaga agcttctgct
    24721 gaggtagagg cctgattttc ttgtggaact tcttctggca tcactggaac cgttgccgct
    24781 gctttttctt gaaggctttt ttcaagagca gccttacgac gatagtagtc ttccataacc
    24841 tgttggctac tggattgctc ttttgaagtg tctactgctt cttcattcgc cgaatcattt
    24901 gaggcatcag cttgagcttg ttttgttgga aactgttcca caactggctg gtcaacaagg
    24961 tcttttggtt ctgatgccgt agattgacta agttcactta ccttaaggtc tgttttgtcg
    25021 gcagcaaact cattggtatc ttctgatgga tgattactat tgtcacctgt ttcttcctca
    25081 acctgtgcgt ttactgaagc tgcttcggga ccattttcca ccgaacctgc gccctctgaa
    25141 gaaacgtcct cttgttgaac cacctcaatc ttgttttgct cattggcttt ttcagaaagc
    25201 tcctctttct gagttgtttc tgtcaaaaga ttttcttcta acttttcctc agctaccttc
    25261 ggggtttctt ttttcccaaa taaacgatca aataatccca ttaactctcc tcttctaaaa
    25321 taaatgtctt cacagcttct gcgactccag actcatcatt ggttaaggtg gtcacggcat
    25381 cggcaacggc tttagctcct gaaacaccat tagccatagc tactccaagt cctgcccatt
    25441 ctaacatcgc aaagtcgtta gcttcgtctc ccatagccat gacatgtcta gcgtcaaggc
    25501 caagatgctg gcaaagcaag tttaatccaa aagctttatc aatgcctttt ggcatgattt
    25561 caaaaataat atctcgtgat ttaaaggctt caaaatcctc aaataagctt ggggatagct
    25621 ttataatctg ctgatccaaa aagtctggat cggtgactgt cacaattttg ttataggtaa
    25681 tgtcttttgg tagttccgct acagatgtca cctcaataaa ggtcaacagt gggttagcta
    25741 gatggtactg cgaatgccta ccgtccttac ttggaatgct atacacatcc ccaccactga
    25801 taatatctgt tggtaagcca accgcctcta aagcttgctg aatctggcaa acctggtcaa
    25861 aactgagaga gctcttatcc aaaacctccc ctgtatttcg ctgaaccaaa cccccattaa
    25921 aggtaatact gtagtcatcg tggtcaagca agtccaactc ttctagcaag ttaccgatgg
    25981 cctttagagg gcgacctgtg gtaataacca ccttaacccc tttttctcta gccgctgcta
    26041 aggctttttt attggcatct gtgacaattt tttcagtgtt gtagagggta ccgtctaaat
    26101 caagcgccaa aattctaata ttcatgagtt acactagtcc ttttaaatac gttagtactg
    26161 ctccgtcatt acagtggcca atgacttggt ctgaaatcac tttgatttca ggtcttgcat
    26221 tttccgtagc aatagcccta ccagcaaact ccaacatctg gtaatcatta aaattatccc
    26281 caaaagcaat cgtttctgct ttcttaatgc ccaaggcttg acaaagatgc tccataccaa
    26341 agcccttatt cacctctttt aagataatat caatggaatc aaagccggtg gtcactgcag
    26401 tggcataagg gagggcttga ttcaaccaat cgcttccttc taagacggta tggcctgtga
    26461 agttggtaga aactttaaaa atggcgtcat tctccatatc ttcaaaacca ttaataacct
    26521 taacattagc atagtaatgt tttgtttttt gaatgtactc ttctgatgcg ccttttaaaa
    26581 tataggctgc cttttgccca gaaaaaacca tacctgtttc tacatagtga ggattggcta
    26641 aaaccttttt tgcaacttcg gtatactgct ctttggtcat catatctgca aatagaatct
    26701 caccatggta ttgaaccacg gaaccatttt ctgcaataac agcaatctga tccaaaaagg
    26761 gctcaaagag ctgatcaatg gctaagagcg agcggccact cgaaaccgca aataaaatgc
    26821 ctttttcggc taactttggc aagagcgcag ctagttgctc ttgattataa gtaccatctt
    26881 cagctaggaa ggtaccatcc atatctgttg ctattagttt aatcatacaa tgtctccttt
    26941 ttttactcta ggggggtgca aacgccattt agggtgtgca ctttttaacg atttaaataa
    27001 gccccatcat ttctgcattc tgttcaattt ttcttattat atcataaaaa gcagctttca
    27061 ttccattcgt aaaaaacagc cagattatat ttcgataacc tgaccgtttt tgaagtgaac
    27121 tgctactttt ttatttttat aaatgaccac tggatgtata ctataaaagt agacaaaact
    27181 ttgtgtgtta caatctgttt taaaagacac aaaaacgaaa ggacaaccta tgtcaacagt
    27241 taaacgctac gatgaagaat tcaaacaatc tcttgtcaac ctttatcaaa ctggaaaaac
    27301 tcagtctgaa ctctgtaaag actatggggt atccgcttct gcgcttgcaa aatggatcaa
    27361 acagtattct caagtcaaac tcgaagataa ttctgtactt actgccaagc aaatacaaga
    27421 gctacaaaaa cgtaatgcgc agcttgaaga ggagaacctt atcctaaaaa aagcaagtgc
    27481 catattcatg caaaacttaa agtaagactc ctcgctgtct atcggttacg ctttgaacac
    27541 gacacaacaa ccttatgtcg tgttttacgt gtcaatcgct ccacttacta taaatttcta
    27601 aaacataagc cctcaaaaag agaattggat aatcaaattt atagaaaaca aatacttgag
    27661 atttatacca aagcaaacaa aagacttggt gtgaagtcta tcaaggtcat tcttcaaaga
    27721 gactacgaca caaaaatctc tgaaggaaga atttaccgtc ttatgaagaa tatggcgctc
    27781 cctaaaatgg ctaccgttaa gccaaaaaca gctcttaaaa ggactcaaaa aacgtatcct
    27841 caaaacttac tcaaccagaa atttaatcct gacaaaccaa atcaagtatg gtctactgac
    27901 ttcacctata tttctattgg atataagaaa tatgtctatc tctgcgcaat acttgatctc
    27961 tattctagaa aatgtattgc ttggaaactg agtcatcata tggatgcaaa gttagcatgt
    28021 gacactctag aattagctct taataaaaga aagattgaag gaacacttct ctttcattcc
    28081 gaccaagggt cacaatttaa ggccaggaaa ttgagaaaaa taattgatga caacactatc
    28141 atgcattctt tttctaaacc tggatatcct tatgataatg ccgtaactga agcatttttc
    28201 aagtatttaa agcatagaca aatcaaccga aaaaagtatc aaaatatcaa acaggttcaa
    28261 ttagactgct ttgaatacat tgagaatttt tataacaatt acaacccaca tacggctaat
    28321 ctaggactaa cccctaatca gaaagaagaa aattagttta acgtaataaa ataacacggt
    28381 tttctgtcta cttatttgac attagtccaa aatacatcct aatggtgtcg ttttttttat
    28441 ttcactagac attcgttcta gtttaggata taagaaggtc tcaaattttt ttcatgatgt
    28501 ttattccaaa catccaatca cctttctata taactgttca tttagtggta tagacaaaca
    28561 gaaatactcc gtttctagtt tcatagaaga cggagtattt tttagtagag acgtctggat
    28621 tgatagatag ttggtatata gctcatccct actaggcttt cattatagac tatggtgggt
    28681 ttcgttgaca acaatatgtg attattattc taaatgacag tatacatcaa ctatcaagta
    28741 tgcttttaat tttatttttt aaagctacag atgaaaat
//
LOCUS       DATXQK010000019        27717 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.019, whole genome shotgun sequence.
ACCESSION   DATXQK010000019 DATXQK010000000
VERSION     DATXQK010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27717
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.019"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            256..1008
                     /locus_tag="VC394_000924"
     CDS             256..1008
                     /locus_tag="VC394_000924"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEP5994402.1"
                     /translation="MIDIMSDFQNKTCSKNNFIVGPCSIESYDHIRLAASSAKKLGYN
                     YFRGGAYKPRTSAASFQGLGLQGIRYLHEVCQEFGLLSVSEIMSERQLEEAYDYLDVI
                     QVGARNMQNFEFLKTLSHIDKPILFKRGLMATIEEYLGALSYLQDTGKSNIILCERGV
                     RGYDVETRNMLDIMAVPIIQQKTDLPIIVDVSHSTGRRDLLLPAAKIAKAVGANGIMM
                     EVHPDPDHALSDAAQQIDYKQLEQLGQELWQD"
     gene            1064..2137
                     /locus_tag="VC394_000925"
     CDS             1064..2137
                     /locus_tag="VC394_000925"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEP5994403.1"
                     /translation="MPQTLHVHSRVKDYDILFTDHVLKTLADCLGERKQRKLLFITDQ
                     TVYHLYQTLFEEFAQQYNAFVHVCPPGGQSKSLERVSAIYDQLIAENFSKKDMIVTIG
                     GGVVGDLGGFVAATYYRGIPYIQIPTTLLSQVDSSIGGKVGVHFKGLTNMIGSIYPPE
                     AIIISTTFLETLPQREFSCGISEMLKIGFIHDRPLFQQLRDFQKETDKQGLERLIYQS
                     ISNKKRIVEQDEFENGLRMSLNFGHTLGHAIESLCHHDFYHHGEAIAIGMVVDAKLAV
                     SKGLLPKEDLDSLLQVFERYQLPTTLERADVSATSLFDVFKTDKKNSEQHIIFILPTE
                     TGFTTLAINKDDHQFVEKLDSLL"
     gene            complement(2572..2877)
                     /locus_tag="VC394_000926"
     CDS             complement(2572..2877)
                     /locus_tag="VC394_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEP5994404.1"
                     /translation="MIENLSQLVQYHDHQVASRSLSRLLGLSQSIVLYAMAQGESISQ
                     ETSPRDKVILVLEGQLIFDLEDQKQVLTQESLIAIPAQKAHHLEAKTDCKLLQVLLD"
     gene            complement(2879..3217)
                     /locus_tag="VC394_000927"
     CDS             complement(2879..3217)
                     /locus_tag="VC394_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002265614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEP5994405.1"
                     /translation="MSYINNIEHAKVLDLTQEVMIEQDQMLSRTLVQRQDLGITVFSL
                     DKGQEIGRHSSPGDAMVTILSGLAEITIDQETYRVAEGQTIVMPAAIPHALYAVEAFQ
                     MLLVVVKPEA"
     gene            complement(3270..4025)
                     /locus_tag="VC394_000928"
     CDS             complement(3270..4025)
                     /locus_tag="VC394_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEP5994406.1"
                     /translation="MVTKEVGHDFLARLGKKRLRPGGVEATNWLIEQGQFSQDCHVLE
                     VACNRCTTAIELAKTFGCQIDAVDLNPKVVKEAQERVTVEGLTRHITVSQANALSLPF
                     PDNHFDIVINEAMLTMLNAKAKEKALNEYWRVLKPGGRLLTHDVAYEDPKTRQVLDEL
                     RQTIHVNVAPLSLTGWQALYQEHGFSQVTYHHGKMSLMTPRGMIKDEGLMNTLNIMRR
                     GLKKENRQQFLKMRRFFTRTGKNLCYIAVKSVK"
     gene            complement(4277..5155)
                     /locus_tag="VC394_000929"
     CDS             complement(4277..5155)
                     /locus_tag="VC394_000929"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020905350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEP5994407.1"
                     /translation="MSERLSGHTLLVSLLATPIRHSLSPKMHNEAYAKLGLDYAYLAF
                     EVGTEQLADAVQGIRALGIRGSNVSMPNKEAILPLLDDLSPAAELVGAVNTVVNKDGK
                     GHLVGHITDGIGALRALADEGVSVKNKIITLAGVGGAGKAIAVQLAFDGAKEIRLFNR
                     QATRLSSVQKLVTKLNQLTRTKVTLQDLEDQTAFKEAIRESHLFIDATSVGMKPLENL
                     SLITDPELIRPDLVVFDIVYSPAETKLLAFARQHGAQKVINGLGMVLYQGAEAFKLIT
                     GQDMPVDAIKPLLGDE"
     gene            complement(5293..8799)
                     /locus_tag="VC394_000930"
     CDS             complement(5293..8799)
                     /locus_tag="VC394_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HEP5994408.1"
                     /translation="MVYRQKSSEIVTRTITKPSRATSNVKQEIDMTPDSKEQTVTGYQ
                     YHYIDQEGRKQPFNQGWRFLMADVACAQDPSFDDSNWQVIHLPHDFSLTQPYTRNGEA
                     ESAYKLGGVGWYRHYLVLDEVLAGCHVAITFEGSYMETEIYVNGQFIGKHLNGYQEFT
                     YDISDVVTFGAENLLAVRVENKVPSSRWYSGSGLYREVSLSVLPQLHFVADQVAMTLA
                     DTAVQEKGQQKVDLRFALNQSIQTCHYQLSLCLWEQSHCSKDKKLLYQETEVPLADLA
                     FQRQYGLTLSLEELQLWSPDNPHLYDLELTLYYQGQVIDCFCLETGFRQLTFMANQGL
                     FVNGRAVKLKGVCLHHDQGGLGACAYEDALARQLVLLKDMGANTIRSTHNPSSPKLRQ
                     LANRLGFFVIEEAFDTWTYAKNGNVNDFSNYFHQTIGTENASYLQRVRSPETSWAQYS
                     IEAMVWSAKNDPSVLMWSIGNELMEGFSADVSHYPELTRQMCQWIAAIDTSRPITFGD
                     NKLKEADFCWHEEVSQMATLLSQLDHPQGLIGLNYANGKDYDKLHEEHSDWLLYGSET
                     ASAITSRAYYKETKKVLDSGYHLTSYDHAKVDWGAFASQAWYDTITRDFVAGECVWTG
                     FDYLGEPTPWNKIDSGVVGLWPSPKNAYFGILDTAGFPKDSYYFYQSQWAQGQTTLHL
                     LPVWQKDQLCFDEQGLVEVVVYSNAASVQLMFEDEQGNVTDYGRKAFHTYSTPTGHTY
                     QLYQGADAAKNPHENLYLTWRVPYQKGLLRAVAYDISGKSIPKTSGRSQVRTYGSVAK
                     LSWKAFEAPIDAPWELLYLDLSLLDSRGELVSHAQDWLKVQVEGPAHLLALDNGNPTD
                     HTPYQEPLRQAYGGKLLAILALTGEAGHIKVTAKVSQQLSSVFQTQVACRAPKEHLVT
                     LPLEKYQFKKSRLTSQQEIGQSCLKTTQFLSVLENKDTQQKAGKVSTPHILITSYQHY
                     LSLPLQVCSQAFDFKKGDDIFLPQWLQVKDAQGSFVKKAFPMTWYLKEKIFAKPILVT
                     GYVSCFGQSIPVEARLNLAQASKRRHQDLSLVAKQSLATSQKRLTYCYQYDTVQAIGA
                     ISLQNHTDTSLSVSVAISYGSQSSIKQETLMLADSHLSQLEEVIFFDDILTVLYLVVT
                     LEVIEGPDLTEDSIQIGLWDLA"
     gene            complement(8729..10213)
                     /locus_tag="VC394_000931"
     CDS             complement(8729..10213)
                     /locus_tag="VC394_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP5994409.1"
                     /translation="MYKVLLVDDEYMILQGLTMIIDWQALGFDVVSTARSGQEALAYL
                     TQYPVDVMISDVTMPGMTGLDLIEAAKTNHPQLQTLILSGYQEFSYVQKAMELETKGY
                     LLKPVDKAELQAKMKQFKDWLDAQQAESIRQETYHDSLLTLWLTDELSEKEFHQLSQD
                     LPEHALTGFTVLYVECSEWIEEMEAYFKQEEQPCYLKKEEDKKIYLAILLGRAQQAKA
                     FTSNLQKQFSENIQQIILGETVDDWENVYESYNQVRQSLFYNSQMSQPTDKGNSKLEM
                     PESRLQFFAFNKALMIGDEPTILAKLEAIFHEMKALNFSPEDVKHVSFLLFSDIYRQF
                     PILDKMTYLSMVKTIHNSQSIDRILRELKKVLDVTNQNNSPEKRYSDLVSETIDCIRK
                     EYHQELTLKAIADRLHVNGVYLGQCFKNETERSFTQYLNHVRIQKAQQLLLYTNQSIN
                     EIAYETGYNTNHYFIKMFKKLNGLSPKEFRDRYKDNYQAIKGDQ"
     gene            complement(10213..11937)
                     /locus_tag="VC394_000932"
     CDS             complement(10213..11937)
                     /locus_tag="VC394_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEP5994410.1"
                     /translation="MSVKTLGRYVTNHFKQGFLINRLMKLYSLLIVSFFTLAAIGLSS
                     YSISSTYKRVDAEAQMRLEETLGRLQLQNDITLRVLDQLVGRSEDYHNLYQYMTLTPN
                     QYFSNVFEDWEKGKDTVLFSEEVRRLFDLYPDVTSMTLLLEDSSAYLYADRVIKTGRL
                     LYSKPEKVTGNVLVRSIRNPESGDVTGRLYLTFDKPPTLVETQQDHYLATFAFDYYGR
                     KLFHQGGRRFPHLEAEVKKAIKADRAADLSNLSKAYRMQYNRSGDLLAYVAVRKSYLL
                     AEAVRTVFVYGLVSLLLAWLLLQLLFRVFRNYIQQVSEITDTVEMVAAGDLSLTIDNS
                     HMELELYHISEAINQMLASIKAYIDEVYVLEVEQRDAQMRALQSQINPHFLYNTLEYI
                     RMYALSCQQEELADVIYAFASLLRNNISQDKMTTLKEELAFCEKYIYLYQMRYPDSFA
                     YHVKIDESIADLAIPKFVIQPLVENYFVHGIDYSRHDNALSIKALDETDHLLIQVLDN
                     GRGISQERLADMEKRLQEHQTTGNSSIGLQNVYLRLFHHFRDRVSWSMAKEPNGGFII
                     QIRIRKDV"
     gene            complement(11927..12532)
                     /locus_tag="VC394_000933"
     CDS             complement(11927..12532)
                     /locus_tag="VC394_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEP5994411.1"
                     /translation="MNGIEKTCFLIWKMIQLTLIFHLLSLAGLIVFGVGPAWQTIVTL
                     FLKTPQEENHYSLKRAVQLWKSYFKEANLRFGLFALTFLFLTFNLHWAVQFPSLFWFT
                     VSFLIVIAMVWLTITYLYMVFYAVSYEINLWNNMKLAFISVFLSFKSFLLMLSVLLGI
                     TLMTWQYKGLYLFLTFGALVFCLDFVTKANRRLVDGVIDEC"
     gene            complement(12838..14283)
                     /locus_tag="VC394_000934"
     CDS             complement(12838..14283)
                     /locus_tag="VC394_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEP5994412.1"
                     /translation="MKKWQKIVCVTGTVLAASSLAACESKSASKDSDVKLLMYQVGDK
                     PDNFDELMTIANKRIKEKTGATVDLQYIGWGDWDDKMSTIIASGENYDIAFANNYVVN
                     AQKGAFADLTTLMPKYAKKTYKNLDPAYIKGNTIDGKLYAFPVDANVYAQQMLSFNKE
                     LVDKYGLDISNIKSYADAENVLKQFHEKEPNTAAFAIGQVFSMSGDYDYPLTKTQPFA
                     VKIDEGKPTIINQYEDESFKNNLRLMHKWYKEGLIPTDAATNTEGYPLEGNTWFMREE
                     TQGPMDYGDTILTNAAGKDIVSRPLTKPLKTTSQAQMANFVVSSVSKNKEKAVEVLSL
                     LNSDPELLNGLVYGVEGKAWEKIGDKKIKLLDGYQPKMHMGAWNTGNNKILYTQESVT
                     DDMIAKRDQSIKDAKESPILGFTVDTKVIKTELSNISNVMNRYKASINTGTVDPDEAL
                     PKLLADLKGAGWDKVQKEVQKQLDDFVAKDK"
     gene            complement(14364..15290)
                     /locus_tag="VC394_000935"
     CDS             complement(14364..15290)
                     /locus_tag="VC394_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEP5994413.1"
                     /translation="MEKKKKVEKVNVRTFDRKTNAIFNILIGLFAISCIIPFIFVIII
                     SFTDESYLINHGYSFFPDVWSTKAYQYIFQGAMSHRIMRSFGISVFITVVGTFINTTM
                     TSTYAYAISRPYFPYRRFFTVYALITMLFAPGMVANYLVVSNLLHLKDTVWALILPMA
                     LGPFGILVMRTFFKKTVPDSIIESARMDGASEWMIFMKIVLPLAVPGIATISLFSALT
                     YWNDWFNALLYVQSENLYPMQYLLMKIQSNLQALAQNAGMSAQMADSLASLPKESVRM
                     AIVVIATLPIALTYPFFQKYFVGGLTIGGVKE"
     gene            complement(15300..16250)
                     /locus_tag="VC394_000936"
     CDS             complement(15300..16250)
                     /locus_tag="VC394_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004219264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEP5994414.1"
                     /translation="MTRQTNKKTSFWKNVIKYRALLLMVLPGFIWFIFFFYIPVLANV
                     VAFKDFHYSAGGFMESLKESPWVGLANFKYLFASKDAWLITRNTIAYNVIFLLFNVFF
                     AIVFAIIMSELRNKRTVKVYHTMSLLPYFLSWVVIEYFVSAFLNTDKGFINQLLVGSG
                     ADPIKWYSNPTWWPLILLFMSGWKGLGYNSIIYYASVKGISDTYYEAAMVDGASKWQQ
                     IRNITIPQLLPMMSILLIINIGNIFKSDFGLFYVIPKNSGPLYDVTSVLDTYVYNALT
                     ATGDIGMASAASLYQSVVGTSILLVTNAIVRRMDPDAALF"
     gene            16446..17324
                     /locus_tag="VC394_000937"
     CDS             16446..17324
                     /locus_tag="VC394_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEP5994415.1"
                     /translation="MSLLCIDIGGTSLKFALCHNGQLSQQSSFPTPSSLEKFYQLLDQ
                     EVARYSAYHFSGIAISSPGAINKEKGVIEGASAIPYIHHFKIQEALEERLHYPISIEN
                     DANCAALAEATLGAGKGASSLAMLVLGTGVGGSLVIDGKIYHGAHLFGGEFGFMIMND
                     RYQTFSQLGTVVNMAKRYSAIVNNGKDYTGKAVLALAEQGDHLALKERQVFLQSLAIG
                     IFNIQHAFDPQLILIGGGVSQADFLLSAIEAELDKLYQAVEISDLRPQLAICHFKNEA
                     NLLGAAIDFYQEHKEA"
     gene            complement(17935..19377)
                     /locus_tag="VC394_000938"
     CDS             complement(17935..19377)
                     /locus_tag="VC394_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEP5994416.1"
                     /translation="MLAFPKEFWWGGATSGPQSEGRFAKQHRNLFDYWYEEEPDLFYD
                     YVGPDTASDAYHQIESDLALLASLGHNSYRTSIQWTRLIDDFEQVTINPDGLAYYNRV
                     IDACLANGIRPVINLHHFDLPIALYQAYGGWESKHVVDLFVAFSKVCFEQFGDRVKDW
                     FVHNEPMVVVEGSYLMQFHYPAIVDGKKAVQVAYNLALATAKVIQAYRQGPAELSDGR
                     IGTILNLTPAYPASQSEADMAAAHFAELWNNDLFMEAAVHGKFPEELVAVLKKDGVLW
                     QSTPEELALIAENRVDYLGLNFYHPKRVKAPDVIPVISPSWSPEWYYDAYLMPGRRMN
                     VDKGWEIYPEAVYDIAIKMRDHYDNIPWFLSENGVGISGEDRYRDETGQIQDDYRIRF
                     LKEHLTYLHKGIEAGSNCFGYHVWTPIDGWSWLNAYKNRYGLVENNIHTQVRRPKASA
                     YWFKKVATHNRLISLEVMEEFGGSASHLSD"
     gene            complement(19402..21096)
                     /locus_tag="VC394_000939"
     CDS             complement(19402..21096)
                     /locus_tag="VC394_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylglucosaminidase"
                     /protein_id="HEP5994417.1"
                     /translation="MTIYHALKDYQEVMTRGDYLVFDMPLTCRFIGRFFRFENQKALL
                     AELATSKYFQWIEEGQAELTVKHFFNRQLDKDAFTLKVSEDKEIIIESQNLRGFRYAQ
                     EALLKVMTFKGDKLYLPIVSVKHSPSFAMRGIIEGFYGTPWTREERLDCLRFIGRKRM
                     NTYMYAPKDDDYQRKLWRDLYPEDWVTYFKELLAVAKEEGIDFWYMISPGLDFDYTKE
                     ADYQLLYQKLQQLLALGVCHFGLLLDDIDYQIVDAVERRFKKTAYAQAHLATEVHHFL
                     NQQHAAPELVICPTEYDNHHDSIYLQELSERIPKEVAFFWTGPSTLASQISQADIETM
                     AAVYQRPIIIWDNIPVNDYQKDPERLFLTPFANRSPFLCQSDYQVKGIVSNPMISWEL
                     SKLTLTDMSHYLWDANRYQPSNSWLETLTDYTGDAELALALQAFAWHNGNRHLHRDLP
                     FEVEEALLAKDVSTLSAWVDELVERVNTLRKLDKPAFQQAIVPWFERVAKDQDFWQAI
                     LEQGPHLETLYADLQEDKHRIGSDIPSRYYRIYYQQQDKLTANQGQVTQARPEDYA"
     gene            complement(21147..22187)
                     /locus_tag="VC394_000940"
     CDS             complement(21147..22187)
                     /locus_tag="VC394_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEP5994418.1"
                     /translation="MTKLLYKKIYHFIKEQIASGRLQIGDRLPTEKELSEQFSVSRIT
                     SKRALVELEQEGLITRSRGKGSFVAEKQVTSPEANKDLLLILPFTSDYELGDYAKGIM
                     TSIAETGYRLMVQLASTVRLDTLSDYAGIIYYPEDVNHSIDFLFYCDHHHIPLVLLDK
                     SLDLFQFPSVVADNKGGACQLTQHLIEQGCDQISFVATGSFGEVSSVRDRYLGYLAAM
                     AESSLKSHYFPKTETETTDAYLDRLVKTLSETTATKRGLVVENDWLAIQLIQKITQVG
                     LYIPDQVAIVGFDNSQASRLLSPKLSTAAQDFYQMGQEAAKVLLQIIHEPDQAVVSCQ
                     LPVQVFIRESSH"
     gene            22320..23606
                     /locus_tag="VC394_000941"
     CDS             22320..23606
                     /locus_tag="VC394_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEP5994419.1"
                     /translation="MTRPIPTSVSQFMAKVESLCGDQHPDWALNFKTSFTNTLETTLK
                     TYEDGTSFLLTGDIPAMWLRDSTAQMKPYLFLAKEDEEIRKIIAGLVKRQFRYICIDP
                     YANAFNEEANGKGHQTDRTQMNPWIWERKYEIDSLCYPIQLAYLLYRETGSTDQFNDD
                     FHRGVELILDLWTVEQDHAQSPYLFERDTWRKEDTLTHAGKGSPVAPTGMTWSGFRPS
                     DDACQYGYLIPSNMFAVVVLSYLEDLYNNLFHNEPVATRAKQLKEAIQSGIADHALVQ
                     NSKGETIYAYEVDGLGQFSIMDDANIPSLLAAPYLGFCAKDDPIYLATRRTILSQENP
                     YYYQGNAAAGIGSSHTPENYIWHIALALQGLTALDQDSKKEMLDLLVATDAGTHLMHE
                     GFDVNDPYQYTREWFSWANMMFCELLLDYLGFSITP"
     gene            23621..26326
                     /locus_tag="VC394_000942"
     CDS             23621..26326
                     /locus_tag="VC394_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEP5994420.1"
                     /translation="MATKKVHIISHSHWDREWYMAYEQHHMRLINLIDDLLEVFQTDP
                     DFHSFHLDGQTIILDDYLQVRPEREPEIRQAIASGKLRIGPFYILQDDFLTSSESNVR
                     NMLIGKEDCDRWGASVPLGYFPDTFGNMGQTPQLMLKAGLQAAAFGRGIRPTGFNNQV
                     DTSEKYSSQFSEISWQGPDNSRILGLLFANWYSNGNEIPTTEAEARLFWDKKLADAER
                     FASTKHLLMMNGCDHQPVQLDVTKAIALANQLYPDYEFVHSCFEDYLSDLANDLPDNL
                     STVQGEITSQETDGWYTLANTASARIYLKQANTRVSRQLENITEPLAAMAYEVTSTYP
                     HDQLRYAWKTLTQNHPHDSICGCSVDSVHREMINRFEKAYEVGHYLAKEAAQQIAGAV
                     NTSDFPTDSQPFLLFNTSGHSKTSVTELTLTWKKYYFGQRFPKEVYQEAQEYLAGLSQ
                     SFQVIDVSGNTISEADILDTSIAFDYDLPKRTFREPYFAIKVRLRLPITLPAMSWKAL
                     ALQLGNETAPSATVPLYDDSNQCLENEFLKVMIQTDGRLTITDKQSGVSYQDLLRFED
                     CGDIGNEYISRQPTHDQPFYADQGTTKLNIISNTAQVAELEIQQTFAIPISADKLLQA
                     EMEAVIDITERQARRSQEKAELTLTTLIRMEKNNPRLQFTTRFDNQMTNHRLRVLFPT
                     HLKTDHHLADSIFETVKRPNHPDATFWKNPSNPQHQECFVSLFDGENGVTIGNYGLNE
                     YEILPDTNTIAITLLRSVGEMGDWGYFPTPEAQCLGKHSLSYSFESITKQTQFASYWR
                     AQEGQVPILVEQTSHHKGSLRAEHSYVTGADDQVALTAFKRRLADNALITRSYNLSND
                     KTCDFSLSLPNYNAKVTNLLEKDSKQSTPSQLGKAEILTLAWKKQ"
     gene            complement(26427..>27717)
                     /gene="rocA"
                     /locus_tag="VC394_000943"
     CDS             complement(26427..>27717)
                     /gene="rocA"
                     /locus_tag="VC394_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator RocA"
                     /protein_id="HEP5994421.1"
                     /translation="LKLFSFVSAIPLRLKNIFYLSLSMVLFQVVFWAFFPDHFILDVV
                     MLAQFLFFALIALYYGKSIKAKFLMFYAFFPLVSISLVKRFIVFFVMPLFGMPYSVVK
                     HNTLLIYSITCFSIFLIYRCIQVFHFDFSTWRQYFQSHRASKLLVFTNSSMALYYLCV
                     QGIDVMSPSLSGLATTTARSIIVLFYFILFLTLLIHLERYVKQNSIEAIVQQKEYREL
                     INYSQHLGLLYQDIQELRQLLTTVSSRLKIGIEQNDISIVRLTYEGILNAEKNNAKDD
                     RLDLTCLDKLQVEAIRHIVLAKLIEAKNKKLKVEVSIPNCIATFFLEVVDFTKLLSFL
                     LDNAIEMSLETKQPCLSIAFLDQNHKLVIVIQSSTKQGQNDSQSVFATPALKKRDDWQ
                     FDLRNVTTILNRYDYLTISSQIHDGILTQLIEIAKPD"
BASE COUNT         8297 a         6047 c         5334 g         8039 t
ORIGIN      
        1 ttcccggtgg tggcatctat aaaaaatact gtcctaataa gatttggggc agtatttttt
       61 tatagctagc aagccttttt taataaaaat gactaagaac atagacaatc tagggtttac
      121 ttgctatttc atcttgttta tggtatcatt tcacaggcta agggtgcttc atggataaca
      181 acgagatttt gtattcctaa ataagacgtt atcccataga gcacgccata cagaattagt
      241 aaaggaatca aacgaatgat tgacattatg tctgactttc aaaataagac ttgttctaag
      301 aataatttta ttgttggacc ttgttccatt gaatcttatg atcacattag attagcagct
      361 tcaagtgcca aaaaacttgg ttacaactat tttagaggag gcgcctataa accaagaaca
      421 tctgctgctt cttttcaagg ccttggcctg caaggaattc gttacttgca tgaggtttgt
      481 caggaatttg gcttactatc ggtcagtgaa atcatgtccg agcgccaatt agaagaagcc
      541 tatgactatc tcgatgtcat tcaggttggt gcccgcaaca tgcaaaattt tgaattttta
      601 aaaaccttgt cacacatcga taagcctatt ttgtttaaac gaggcttgat ggctactatc
      661 gaggaatatt tgggcgccct ttcctactta caagacactg gaaaatctaa cattatctta
      721 tgcgaacgtg gcgttcgtgg ctacgatgtg gagactagaa atatgcttga tattatggca
      781 gtccctatta ttcaacaaaa gacagattta ccgattattg tggacgtgtc tcattcgaca
      841 ggcagacgag atttactctt acctgctgct aagatagcga aggctgtcgg tgccaatggc
      901 atcatgatgg aggttcatcc tgatcccgac catgcgctgt cagatgctgc ccagcaaatt
      961 gattataagc aattagaaca gttggggcag gaactttggc aagattaagc tctaaatatc
     1021 actgcgttat ctctgttacc tagtactgat gaaaggagtg cttatgccac aaacacttca
     1081 tgtccattct cgggtcaagg attatgatat cctgtttacc gaccatgttt taaaaacctt
     1141 agcagattgc cttggtgagc gcaagcagcg taaactcttg tttatcaccg atcaaacggt
     1201 ctaccacttg tatcagactc tctttgaaga atttgctcag caatacaacg cttttgtcca
     1261 tgtctgccct ccgggaggtc aatccaaatc cttggaacgt gtcagtgcta tctacgacca
     1321 gctcatcgct gaaaattttt ccaaaaaaga tatgattgtt actattggtg gtggtgttgt
     1381 tggggatctg ggtggttttg tcgctgcaac ctattaccgt ggtattccct acattcaaat
     1441 cccaacgact ttacttagtc aggtggatag cagtatcggg ggcaaagttg gagtccattt
     1501 taaaggtcta accaatatga ttggcagcat ttaccctcca gaagctatca ttatttcaac
     1561 cacctttttg gagaccttac cgcaacggga attctcctgc ggcatcagtg agatgctcaa
     1621 aatcggcttc attcatgata gaccgctttt tcagcagtta cgtgattttc aaaaagaaac
     1681 cgataagcag ggacttgaac gtctgattta ccaatccatt tccaataaaa aacggattgt
     1741 tgaacaagat gaatttgaaa atggtttgcg catgtcttta aattttgggc ataccttggg
     1801 ccacgcgatt gaatcgctct gtcaccatga tttttaccat cacggtgaag ccattgctat
     1861 cggtatggtg gttgatgcta aactggctgt ttccaaagga ctcttgccaa aagaggactt
     1921 ggatagcctc cttcaagttt ttgaacgtta ccagttacca acaacacttg aaagagctga
     1981 tgtctctgcg acatccctct ttgacgtctt taaaacagat aagaagaatt ctgagcaaca
     2041 tattatcttt atattaccca cagaaaccgg ttttaccacc ctagccatta acaaagacga
     2101 tcaccaattt gtcgagaagc tcgacagttt attataaata gtgactccac tctagctagt
     2161 taagcttgct aagagaataa cggtatcctg gaactcctaa aacatgttac tacaagacaa
     2221 gtcttgataa caatataact tttagctaag aataactaac agcaacatca aaaagaggat
     2281 ttatggaact ataggagaac ggtatcactc gtaaaaagat ctttcttttg cctgtcgttc
     2341 aattatttcg tctaaaaagc cttaatgaga aacctataag gagataggaa actcattaag
     2401 gttttttagt gaattctaaa ataaaccaag ctaaagggct taatctggag atgagaaggc
     2461 caagctttaa aattcgtatt gtcatcgctt tgaatacgta aaaagagaag atgagcgtca
     2521 tcttctcttt tgcttttcca taggccaccc aatacagttg acaataagcc cttaatctaa
     2581 aagcacttgt aatagtttac aatcggtttt agcttctaaa tgatgggctt tttgggcagg
     2641 tatggctatg agactttctt gagttaggac ctgtttttga tcctctaaat cgaaaatgag
     2701 ctgaccttct aaaacaagta ttactttatc tcttggacta gtctcttgac taatgctttc
     2761 tccttgggcc atggcatata aaacaataga ttgagataat cccagtagac ggctcaagga
     2821 tcggctagcc acctgatgat catgatattg caccagttga gagagatttt caatcatgtt
     2881 aagcctccgg ttttactact actaaaagca tttgaaaggc ttctactgca tataaggcat
     2941 gaggaatagc agctggcatc acaatggttt gaccttcagc aacccggtag gtctcttgat
     3001 caatggtgat ttctgctaag ccacttaaga tagtaaccat ggcatctcct ggtgatgagt
     3061 gtcgcccaat ttcttggccc ttatctaagg aaaaaactgt tattcctagg tcttgccttt
     3121 ggactagtgt tctacttaac atttgatctt gctcaatcat tacttcttgc gtcaaatcaa
     3181 gaaccttcgc gtgctcaata ttattgatat aagacatctt cgaactcctc tatggtctgt
     3241 ttagttgtat ggacaggttg gtctaaggtt tattttacac tttttacagc aatatagcag
     3301 agatttttgc ctgttctggt aaaaaaacga cgcatcttta aaaactgttg tctgttctct
     3361 ttcttaaggc ctcgtctcat aatgttcagg gtgttcataa gaccttcatc cttaatcatt
     3421 ccacgaggtg tcatcaaaga cattttccca tggtgataag tcacttgaga aaaaccgtgt
     3481 tcttgatata aggcttgcca acctgttagt gacaagggag caacattgac atgaatcgtt
     3541 tgacgtaact cgtcaagtac ttgtcttgtt tttggatctt cataagctac gtcatgtgtc
     3601 aaaagtcgcc cgcctggttt taacacacgc caatattcat tgagtgcctt ctccttggct
     3661 ttggcgttca acatggttaa catggcttca ttaatcacaa tatcaaaatg gttatctgga
     3721 aatggtagtg ataaagcatt ggcttgggat actgtaatgt gacgggttaa cccctcaaca
     3781 gtaacccttt cttgtgcttc tttgacaacc ttggggttta agtcaacggc atcaatctga
     3841 caaccgaagg ttttggctaa ctctattgct gtcgtgcagc gattgcaggc aacctctaaa
     3901 acgtgacaat cctgagagaa ttgtccttgt tcaatcaacc agtttgtcgc ctcaacaccg
     3961 ccaggtctta agcgtttttt tcctagacgt gctaaaaagt catgcccaac ttcctttgtt
     4021 accattgcta ttctccttta ttcattgaac ttttcctttt ataagagctt cttttctaaa
     4081 aaaacggtag gttatctggc agacctcaaa ggaactatga cttgtatcac gttagggata
     4141 aggctcttca aataagcttt ttactcttaa aaacagctct cgcaaaaaga attttagcag
     4201 cccatgatga ctgctccatc tcttattaat atatcataat taagcttact taaaaagttc
     4261 tttaaaaaag gttgagttac tcatcaccta aaagaggttt aatagcgtca actggcatgt
     4321 cttgaccagt gattaattta aaagcttctg ctccttgata aagcaccatc cctagcccat
     4381 taatgacttt ttgggcgcca tgttgacggg caaaggctag taacttggtt tctgctgggc
     4441 tgtacacgat gtcaaagacc accaagtctg ggcgaatgag ctcggggtcg gtaatcaaac
     4501 ttagattttc taaaggcttc attcctacac ttgtagcatc aataaataga tgagattccc
     4561 tgatggcctc cttaaaggca gtctgatctt ccaagtcttg aagggttact ttagtccttg
     4621 ttagctggtt cagttttgtc acaagctttt gaacgctact tagacgtgta gcttgtcggt
     4681 taaacaaacg aatctctttg gcgccgtcaa aagcgagctg gacagcgata gctttcccag
     4741 ccccaccaac tcctgctagg gtaatgattt tgttctttac cgacactcct tcgtcagcca
     4801 aggctctgag cgctcctatg ccgtctgtaa tatgccccac gagatgtcct ttaccatcct
     4861 tattgacaac cgtattgact gcaccgacca actcagcagc tggggacaag tcatccaata
     4921 agggtaaaat agcttcttta tttggcatag acacattgga gccacgaatt cctaaggcac
     4981 gaatcccttg cacggcatct gctaattgct ctgtccctac ttcaaaagca aggtaggcat
     5041 aatccagccc cagtttagca taggcttcat tgtgcatttt gggagacaaa ctatgacgaa
     5101 taggagtagc aagcaaagag accagcaggg tgtggcctga taaacgttct gacatttaga
     5161 aatctcactt tccatttcaa ccttgtaaag gcgcctttca cgtaataaag gacgcctatt
     5221 tgttttttat tataccttat tttacaaaat ttatcagacc tggtgccaaa gaagtcttct
     5281 tttacttatg cttcatgcta aatcccaaag gccaatttga atactgtctt cagttaggtc
     5341 aggtccttca atgacttcaa gggtcactac tagatacaaa acagtcaaaa tatcatcgaa
     5401 gaaaataacc tcttctaact ggctcaagtg gctatcagct agcatcaagg tttcttgctt
     5461 aatagagctc tgactaccat aactgattgc cacagaaaca ctaagagatg tgtctgtgtg
     5521 attttgcaag ctaatggctc ctatggcttg caccgtatca tattggtagc aataagttag
     5581 ccttttttga cttgtggcca gggattgctt ggcgactaaa ctgagatcct ggtgccgtct
     5641 tttgctagct tgtgctaggt taagccttgc ctcaaccggt attgattgcc caaaacaaga
     5701 aacatagccc gtgacaagaa taggcttggc aaagattttc tcttttaggt accatgtcat
     5761 tggaaaagct tttttcacaa aagaaccttg ggcatctttg acttggagcc actgaggcaa
     5821 gaagatatcg tcgccttttt taaaatcaaa tgcctgagaa caaacctgca agggcagaga
     5881 taaatagtgc tggtaactgg tgataagaat gtgaggtgtg ctaaccttac ctgctttttg
     5941 ctgggtgtct ttgttctcca aaacagaaag gaattgtgtg gtctttagac aagattgacc
     6001 aatttcttgt tgacttgtca gtcggctctt tttaaactga tatttctcta aaggtagtgt
     6061 cactaagtgt tctttggggg cacggcaggc tacttgtgtc tgaaaaacac tgctaagttg
     6121 ctgcgagact tttgccgtga ccttaatgtg tcccgcctct ccagtgaggg ctaaaatggc
     6181 taataatttt cccccatatg cctggcgtag aggctcctga taaggggtgt ggtcagtcgg
     6241 attcccatta tcaagagcca acaaatgggc tggtccttct acttgaacct ttagccaatc
     6301 ttgagcatgg ctgaccaatt cacctcggct atccaagagt gataaatcca gataaagcaa
     6361 ttcccaagga gcgtcaatag gtgcttcaaa ggctttccaa gatagctttg ccactgaacc
     6421 ataggttcta acctgagaac gtccactggt ttttggaatg cttttaccag aaatgtcgta
     6481 ggccactgcc cttagcaacc ctttttgata gggcactcgc caagtcaggt aaagattctc
     6541 gtgaggattc ttagcagcgt cagctccttg gtaaagctga taagtgtgtc ccgttggcgt
     6601 gctataagta tgaaatgcct tccttccata atctgttacg ttgccctgct catcttcaaa
     6661 catcagttga acactggcag cattgctata aaccacaacc tccaccaatc cctgctcatc
     6721 gaaacaaagc tggtccttct gccagactgg taacaggtgc aaggtggtct gcccctgcgc
     6781 ccactgactt tggtaaaaat aatagctatc ttttggaaag cctgccgtgt ctaaaatgcc
     6841 aaagtaggca tttttaggag aaggccaaag gcctacaaca ccgctgtcga tcttgttcca
     6901 gggtgtaggt tctcctaaat aatcaaagcc tgtccaaacg cattcgccag caacaaaatc
     6961 acgggtaatg gtatcatacc aagcctggct agcgaatgca ccccaatcga ccttagcatg
     7021 atcataagag gttaggtgat aaccggagtc aagaactttt tttgtctctt tataataagc
     7081 cctgctggta atggctgagg ctgtttcaga accatacaac agccaatcag agtgctcctc
     7141 atgaagctta tcatagtctt ttccattagc atagtttaac ccaatgagtc cctggggatg
     7201 gtctaattga ctaagaagag ttgccatttg cgacacttcc tcatgccagc aaaagtcagc
     7261 ttcttttaat ttattgtccc caaaggtaat gggacgtgag gtgtcaatag cagcaatcca
     7321 ttggcacatt tgtcttgtta gctctgggta atgactgaca tcggctgaaa aaccttccat
     7381 caattcattc ccaatcgacc acatcaacac tgacgggtca ttcttggcag accagaccat
     7441 ggcctcaata ctatactggg cccagctcgt ttcaggtgac ctcactcttt gaagatagct
     7501 tgcattttca gttccgatgg tctgatggaa atagtttgaa aagtcattaa catttccatt
     7561 tttggcatag gtccaggtgt caaaggcttc ttcaatcaca aaaaagccga gacgattggc
     7621 tagttgccta agtttgggac tacttgggtt atgggtgcta cgaatggtat tagcccccat
     7681 gtctttcaac aggactaatt gtctggctag ggcatcttcg taagcacaag ctcctaggcc
     7741 tccttggtca tgatgcaaac aaactccctt taatttgaca gctctgccat tcacaaaaag
     7801 cccctgattg gccataaaag tcagctgacg aaaaccagtt tcaaggcaaa agcaatcaat
     7861 gacctgacct tgatagtaaa gagtgagttc taaatcgtag aggtgaggat tatctggtga
     7921 ccacaattgg agttcttcca aagacagtgt aagaccatac tgtctttgga aagccaaatc
     7981 agctagaggc acttctgttt cttgataaag gagtttcttg tcttttgagc aatgtgattg
     8041 ttcccataaa cagaggctca attggtagtg gcaagtctga atagactgat tgagggcaaa
     8101 gcgtaaatca actttctgtt gccctttctc ctgcacagca gtatcagcca aggtcattgc
     8161 tacctggtct gccacaaaat gaagctgggg aagaacagac aaactcactt cacgataaag
     8221 accactgcct gaataccaac gagaactggg aaccttgttt tcaacccgga cagccagaag
     8281 attttcagct ccaaaagtca caacatcaga gatatcataa gtaaattcct gatagccatt
     8341 taagtgtttg ccaatgaatt gcccgttaac ataaatttcc gtttccatat aggaaccctc
     8401 aaaagtaatg gcaacatgac aaccagctaa aacctcatct aacacgagat aatgccgata
     8461 ccaaccgaca ccacctaatt tataagcact ttcagcttca ccatttcttg tgtaaggctg
     8521 cgtaagactg aaatcatgtg gtaaatgaat cacctgccag ttcgaatcat caaaagaagg
     8581 gtcctgagca cacgccacat ctgccataag aaaacgccag ccttgattaa agggctgctt
     8641 tcttccttct tgatcaatat agtggtattg gtagccagtc actgtctgct ccttgctatc
     8701 tggtgtcata tcaatctcct gtttgacgtt actggtcgcc cttgatggct tggtaattgt
     8761 ccttgtaacg atctctgaac tcttttggcg ataaaccatt caacttttta aacatcttga
     8821 taaaataatg attggtattg taacctgttt cataagcaat ttcattgatg gattggttgg
     8881 tgtaaagcaa taactgttgc gctttttgaa tcctaacatg atttaagtac tgggtaaaac
     8941 tacgctcggt ttcattttta aagcattgcc ctaaataaac cccattgaca tgtaacctat
     9001 cagcgatagc tttaagggtt aattcttgat ggtattcctt gcgaatgcaa tcaatggttt
     9061 cactaactaa atctgaataa cgtttttcag gggaattgtt ctgattggtg acatccaaca
     9121 ctttttttag ttccctcaaa atgcgatcaa tggattggct gttatgaata gtttttacca
     9181 tggacagata agtcatttta tctaaaatgg gaaattgacg gtaaatgtca gagaacaaca
     9241 agaacgacac atgcttcaca tcctctggtg agaagttcaa ggccttcatt tcatgaaaaa
     9301 tggcttctaa tttggccaag atggttggct catcaccaat catcaatgct ttgttaaagg
     9361 caaaaaattg caaccgagat tctggcatct caagcttact gttgcctttg tcagttggtt
     9421 gtgacatttg gctattgtaa aaaagactct ggcgcacctg attgtagctc tcataaacat
     9481 tttcccagtc atcaaccgtt tctcctagga tgatttgttg gatgttctct gaaaattgct
     9541 tttgtagatt actggtaaag gctttggctt gctgggctct cccaagtaaa atcgccaagt
     9601 aaatcttctt gtcttcttct tttttgaggt aacagggttg ttcttcttgc ttaaaataag
     9661 cctccatctc ttctatccac tcggaacact ccacatacag cacggtaaat cctgttaggg
     9721 catgctcggg gagatcctgg ctaagttgat gaaattcttt ttcactaagt tcatctgtta
     9781 accacagtgt cagaaggctg tcatgatagg tttcttgccg gattgattca gcctgctggg
     9841 catccaacca gtctttaaat tgtttcattt tagcctgcaa ttctgcctta tccacaggtt
     9901 ttagcaaata ccccttggtt tctaattcca tagccttttg cacataggag aattcttgat
     9961 aaccagataa aatcaaggtt tgtagctgag gatgattagt tttagctgct tctattaaat
    10021 ccagtcctgt cattccaggc atggtaacat ctgaaatcat gacatccact gggtactgag
    10081 tcaaataggc taaggcttcc tggcctgatc ttgctgttga aacgacgtca aaaccaagag
    10141 cctgccaatc aataatcatg gttaatcctt gtagaatcat gtattcatcg tcaactaata
    10201 ataccttata cattaaacat cctttctaat cctaatttga atgataaagc caccatttgg
    10261 ctccttagcc atggaccaag aaaccctgtc tcgaaaatga tggaaaagac ggaggtaaac
    10321 attttgcaaa ccaatagagc tattgcctgt tgtttggtgc tcttgaagcc ttttttccat
    10381 atctgctaag cgctcttgac taataccacg tccattatca agcacctgaa tcaagagatg
    10441 atccgtctca tctaatgcct tgatgcttag tgcattgtca tggcgactat aatcaatccc
    10501 atgcacaaaa taattttcaa cgagaggttg aatgacaaat ttaggaatgg ctaaatcagc
    10561 aatactctca tcaattttta catgataagc gaagctatct ggataccgca tttggtagag
    10621 gtaaatgtac ttttcacaaa aagccagttc ttcttttaag gtggtcatct tatcttggct
    10681 aatattgttg cgaagcaaac tcgcaaaggc ataaatgaca tctgctaatt cttcttgttg
    10741 acaactaagg gcgtacatcc gaatatactc taacgtgttg tataaaaaat gagggttgat
    10801 ttgagactgc agagctctca tttgggcatc tcgttgctct acctctaaca cataaacttc
    10861 gtcaatataa gctttaatgc tggccaacat ctgattaatg gcttctgaga tatgatacag
    10921 ttccaattcc atgtggctgt tgtcgatggt taaagacaaa tcgcctgctg caaccatttc
    10981 aacagtatcc gtaatttcag aaacctgctg gatataattt cggaaaaccc taaagagcaa
    11041 ctgcaataac agccaagcta agagtagaga aacaaggcca tacacaaaaa ctgtcctgac
    11101 agcttccgct aataaatagg attttctgac agccacataa gctaagaggt ctcccgagcg
    11161 attgtactgc atccgataag ccttgctgag attactaaga tcagctgccc gatctgcctt
    11221 gatagccttt ttaacctcag cctctaaatg aggaaatctc cgacctcctt gatggaagag
    11281 tttgcggcca tagtaatcaa aagcaaaagt agctaaatag tgatcctgct gtgtctctac
    11341 caaagtcggt ggtttgtcaa aggtcaggta aagacgacct gtcacatctc ctgattctgg
    11401 atttcgaatg gagcgaacta aaacatttcc ggttactttt tcaggcttgc tataaagcaa
    11461 acgacccgtc ttaatgaccc tatcggcgta cagataagca ctactgtcct ctagcagtag
    11521 ggtcatagaa gtcacatcag ggtacaagtc aaaaagacgc ctcacctctt cagaaaatag
    11581 gaccgtgtct tttccttttt cccagtcttc aaaaacgttt gaaaaatatt ggtttggggt
    11641 taacgtcata tattgataga ggttatggta atcttctgaa cggccaacca actggtctaa
    11701 aacccgtagg gtaatatcgt tttgtaactg caatctacct agagtctcct ccagtcgcat
    11761 ctgcgcctca gcatccaccc ttttataagt actactaata gaataactgc tcaagccaat
    11821 agctgctaaa gtgaaaaaac ttacaattag caggctatat aatttcatca gtcgattaat
    11881 caaaaaaccc tgcttaaagt ggttagtcac ataccttccc aatgttttaa cactcatcaa
    11941 tcactccatc tactagtcga cgattagcct tagtgacaaa gtccaagcaa aagactagag
    12001 ctccgaaggt taaaaagaga taaaggcctt tgtattgcca agtcattaag gtaattccca
    12061 gcaaaacact cagcatgagt aaaaaggatt tgaaacttaa aaagacgctg ataaaggcta
    12121 atttcatatt attccaaagg ttaatctcat agctcactgc ataaaaaacc atgtaaagat
    12181 aggtaatggt tagccaaacc atggctatta caatcaagaa agacacggta aaccaaaaca
    12241 aggatggaaa ctgtactgcc caatgcaaat taaaggttaa aaaaaggaaa gtcagggcaa
    12301 aaagaccaaa tcttagatta gcttctttga aataagattt ccaaagttga acagcccttt
    12361 tcaaagaata gtggttttct tcttgaggtg tttttaaaaa tagggtgaca atggtttgcc
    12421 aggctggccc caccccaaac acaatcagtc cagctaagga caagagatga aaaatgaggg
    12481 tcaattggat cattttccaa attaaaaagc aggttttttc aatgccattc atatgatagt
    12541 cctctttttc ttgttaacac ttatagcggt ttcaaggacc agcagctacg agactagttt
    12601 tagcaccccc agtaagatgc tggtattagt tttactctga ttaatagttt agactcttct
    12661 aatatagcac actttatagg cttttggaaa ccgctttact aagagtagta acattattta
    12721 cattaatttt tctcccttta taacttccac ttatttgatg atccgttttt tccactcatc
    12781 agaagaaaag agctgagatt tcccagccct caacttataa tcaagaaaat gaatgcctta
    12841 cttgtcttta gctacaaagt cgtccaattg tttttgaact tctttttgca ccttgtccca
    12901 accagcacct ttgaggtctg caagaagttt tggaagggct tcgtcaggat ccactgtacc
    12961 agtattgata cttgctttat atcggttcat gacatttgaa atatttgata attccgtttt
    13021 gataactttt gtgtcaacag tgaaaccaag gattggagat tctttagcat ccttgattga
    13081 ttggtcacgt ttagcaatca tgtcgtcagt aacagattct tgagtgtaaa ggattttatt
    13141 gttaccagta ttccaagcac ccatgtgcat ttttggttga tagccatcga gcagtttgat
    13201 tttcttgtcg ccaatttttt cccaagcttt gccttcgaca ccataaacaa gcccatttaa
    13261 caattcggga tcgctattaa gaagagaaag gacttcaact gctttttctt tgtttttaga
    13321 tacgcttgat accacaaagt ttgccatttg tgcttgtgat gtggttttta gcggtttagt
    13381 caatggacga gacacgatgt cttttcctgc agcattggtc aagatagtat cgccatagtc
    13441 cataggacct tgggtttctt cacgcataaa ccaagtgttt ccttcaaggg gataaccttc
    13501 tgtattggtc gctgcatctg ttggaatcaa accttcttta taccatttgt gcatcaagcg
    13561 gagattgttt ttaaaggact catcttcata ctgattaatg atggttggct tgccttcatc
    13621 aattttcaca gcaaagggtt gggttttggt taatgggtag tcatagtcac ctgacatact
    13681 aaagacttga ccaatagcaa aagctgctgt atttggttct ttttcgtgga attgtttcaa
    13741 gacattttca gcatctgcat aggacttaat gtttgaaatg tcaaggccat atttgtccac
    13801 tagttcttta ttgaaagaaa gcatctgttg ggcataaacg ttggcatcta ctgggaaggc
    13861 atagagttta ccgtcaatag tatttccttt aatataggct gggtctaagt ttttatacgt
    13921 tttcttagcg tattttggca ttaacgttgt caaatcagca aaagcacctt tttgtgcatt
    13981 gaccacataa ttattggcaa aagcaatgtc atagttttca ccagaggcaa tgatggtact
    14041 cattttatca tcccagtccc cccaaccgat gtattgaagg tcaaccgttg cacctgtttt
    14101 ttctttgatg cgcttgttag caattgtcat caattcatcg aagttatcag gtttgtcacc
    14161 aacttggtac atcaataatt tgacatcact atcctttgat gctgacttgc tttcgcaggc
    14221 tgctaaacta gacgctgcaa gcacagttcc agtcacacac acgatttttt gccacttttt
    14281 cataagtgaa gcctcctaaa aaatgttttc ttttgttact aatgattaga taaaatctgg
    14341 tcttgtgacc agttccttaa gtcctattcc ttcaccccac caatggtcaa accaccgaca
    14401 aagtactttt ggaagaatgg gtaagtcaag gcaatcggca aggttgcaat aaccacgata
    14461 gccatacgaa ctgattcttt tggcagcgat gctaagctat ctgccatttg tgcactcatg
    14521 ccagcatttt gtgccaaggc ctgtaggttg ctctgaattt tcatcagtaa gtactgcatt
    14581 gggtagaggt tttcactttg cacataaagc aaggcgttaa accaatcatt ccaataagtt
    14641 aaggcagaaa ataaactgat ggtagcaatc cctggaacag ccaaagggag aacaattttc
    14701 ataaaaatca tccattcact agccccatcc atacgagccg actcaataat gctatctgga
    14761 actgtctttt taaagaacgt tctcatgacc aagatgccaa atgggcccag agccattggt
    14821 aaaatcaaag cccaaaccgt atccttcaaa tgaaggagat tactgaccac caagtaatta
    14881 gcaaccatcc ctggtgcaaa gagcatggtg ataagtgcat aaacagtaaa aaaacgtctg
    14941 tatgggaaat aaggtcttga aatcgcataa gcataggttg atgtcatggt cgtgttaata
    15001 aaggttccca ccactgtaat aaacacggat atcccaaatg acctcatgat tctatgggac
    15061 atggctcctt gaaaaatata ctggtaagcc ttagttgacc agacatctgg aaaaaaacta
    15121 tacccatgat taatcaagta actttcatca gtgaaggaaa taataatcac gaagataaaa
    15181 ggaatgatac aagagatggc aaacagacca atcagaatat taaagatagc attggttttt
    15241 cgatcaaagg ttctaacatt aaccttttcg acttttttct ttttctccat gttttcctcc
    15301 taaaacaagg ctgcatcagg atccatgcga cgaacaatcg cattagtgac taacaagatg
    15361 ctggtcccaa ctactgactg ataaaggcta gctgccgatg ccatcccaat atctcctgta
    15421 gcagtcaagg cattgtagac ataggtatct aacacactgg tcacgtcata aagtgggcct
    15481 gaattcttgg gaataacgta gaaaagtccg aaatcagatt taaaaatatt accaatatta
    15541 atgatcaata agatagacat cattggcaat aattgtggaa tggtgatgtt cctaatttgt
    15601 tgccatttgc ttgcaccatc caccatagca gcttcataat aagtatcaga aatccctttg
    15661 actgaagcat agtagataat gctattgtag cctagccctt tccagccact cataaagagt
    15721 aaaatcaagg gccaccaagt gggattggaa taccacttaa ttggatctgc tccactgcca
    15781 accaagagct gattaatgaa acctttatct gtatttaaaa aggcagaaac aaagtattca
    15841 ataaccaccc atgataagaa ataaggcaac aaggacatgg tgtgatagac cttaaccgtt
    15901 cgcttatttc gtaactcact catgataatg gcaaaaacaa tcgcaaagaa aacattaaaa
    15961 agtaagaaaa tcacattgta agcgatggta tttctggtaa tcaaccaagc atctttggaa
    16021 gcaaagagat atttgaaatt agccaaacca acccaagggc tctccttcaa actttccata
    16081 aagccccctg ctgaataatg aaaatcttta aaagcaacca cattggctaa aactggaata
    16141 taaaagaaaa agatgaacca gatgaaacct ggtaatacca ttaataataa ggcccggtat
    16201 ttaatcacat ttttccaaaa ggatgtcttt ttgttggttt gtcttgtcat ttcccaacct
    16261 ccctttgtgt taatcacgtt atagttacag tatatcaagc gctttcatta ttttaaagca
    16321 acaaattata ggtatgataa cacaaagtat agaatcactt agaaagcctt ttcattgttg
    16381 cttaaatctg tcatactaag atgagacatg atcgtctatt ctactataat aaaggagaac
    16441 acctcatgag cttgttatgt attgatatag gaggcacttc ccttaaattt gccctctgtc
    16501 acaatggcca attaagccag caaagcagct ttcccactcc ctctagtctg gaaaagtttt
    16561 accaattact cgaccaagaa gttgctcgtt actctgctta ccatttctca ggtattgcca
    16621 tcagttcacc tggggcaatc aataaagaaa agggggttat cgaaggcgcc agcgctattc
    16681 cctacattca ccattttaaa atacaagagg ccttagaaga acggctacat taccctattt
    16741 ctatcgaaaa tgatgccaat tgtgccgcct tagctgaagc taccttaggg gcaggaaaag
    16801 gcgctagtag cctagccatg ctcgttcttg gtactggagt tggaggaagc ttggtcattg
    16861 atggcaagat ttaccatggt gcccacttat ttgggggcga atttggtttc atgatcatga
    16921 acgaccgcta ccaaaccttt agtcagttag ggacagtggt aaatatggcc aagcgctata
    16981 gtgcaatcgt caataacgga aaagattata ctggcaaagc tgtccttgcc ctcgcagaac
    17041 aaggagacca cctagcactc aaagaaaggc aggtctttct ccaaagttta gccatcggta
    17101 tttttaacat tcaacacgcc tttgatcctc aactgatttt aatcgggggt ggtgtttccc
    17161 aagctgactt tctactgtca gcgattgaag ctgaactaga caaactctac caagccgtcg
    17221 aaatctccga ccttagaccc caattagcta tttgtcactt taaaaacgaa gccaatcttc
    17281 ttggagctgc cattgatttt taccaagaac acaaggaggc ctaaccatgg accgctaaat
    17341 ccctcttatc ccctattgtc cctaatcgcc tctgacatct acaaagacat cctaaaagga
    17401 ttgctgatta gctacaacga tactcgcttt ctgcctaaat gactctctgc tgatgaacac
    17461 agcctgatgc ctggtccctt tgttgatgtc attgttgctg acgcttctgt taaaggcatt
    17521 ggtcatcagt taatactcga gctattagaa gctatgctag ttatcgccaa taagcaaagc
    17581 ctcacttaat aactcactca tatagtcccc ttatcgtgga cagtgacata acaaacagtc
    17641 actagaaacc atgaggggct tttatctggt ccaaaaatat gaaagaaaat cctaaaatac
    17701 caagatgaaa aacaagcatc cttagccaag atactctaaa gaattgaaga gccaaattac
    17761 ccaagatgga tagatgatgc caaaccagac agaacgctta tatctagttc ggcaaatacc
    17821 aacaccttcc aaagaaatac aagagcctcg atcctcttaa catgaggaaa aaggcccttg
    17881 tttaatcatt ggtaacatca ttattcactt gtctcaaagg agacgttcac acgttcaatc
    17941 actgaggtga gaagcagagc ctccaaactc ctccattact tctaatgaaa tcaagcggtt
    18001 atgagtagct acttttttaa accaataagc tgaagcttta ggtcgtcgca cttgggtatg
    18061 gatattattt tccacaaggc catagcggtt tttataggca ttgagccaag accagccatc
    18121 aattggcgtc catacatggt atccaaaaca attcgagcct gcttctattc ctttgtggag
    18181 gtaggtcaaa tgttctttca aaaatcggat gcgataatca tcttgaatct gaccagtttc
    18241 atcacggtaa cggtcttctc ctgaaatccc tacaccattt tcagataaga accaaggaat
    18301 attgtcgtaa tggtctcgca tcttgatggc aatatcatag acagcctcag gatagatttc
    18361 ccacccctta tccacgttca tgcggcggcc aggcatcagg taggcgtcat aataccattc
    18421 aggactccaa gatgggctaa tcactggaat gacatccgga gccttcacgc gcttagggtg
    18481 gtaaaaattc aatcccaaat aatccactct attctcagca ataagagcca attcttctgg
    18541 cgttgactgc cacaaaaccc catctttttt caataccgca accaattctt ctgggaattt
    18601 cccatgaaca gcagcttcca taaacaagtc attgttccaa agttctgcaa aatgcgcagc
    18661 agccatgtcc gcttctgatt gactagctgg gtaggctggc gtcagattga gaatggttcc
    18721 aatacgccca tcactaagtt ctgctggacc ttgcctgtaa gcttgaataa ccttggctgt
    18781 ggctagggct aggttatagg cgacctggac tgcttttttc ccatccacga tagcagggta
    18841 atgaaattgc attaaataag agccttccac aacgaccata ggttcattgt ggacaaacca
    18901 atccttaacc cgatcaccaa actgctcaaa acaaaccttg ctaaaggcga caaataagtc
    18961 cacgacatgc tttgattccc agccaccata ggcttgatag agggcaatgg gcaggtcaaa
    19021 atgatgcaaa ttaatgacag gacgaatacc attggccaag caagcatcaa tcactcgatt
    19081 ataataagca aggccatcag gattaatcgt cacttgctca aagtcatcaa tcaaacgcgt
    19141 ccactgaatg gaggtgcggt aggaattgtg acctagtgaa gctaacaagg ccaaatcaga
    19201 ttcaatctga tgataggcat ccgaagccgt atcaggtccc acgtaatcgt aaaacaagtc
    19261 aggctcctct tcataccaat aatcaaagag attgcggtgc tgtttagcaa atcgcccctc
    19321 actttgaggg cctgaagtgg ctcctcccca ccaaaactct tttggaaatg ctaacatatc
    19381 attcctttct agctcgtatg actaagcata atcttcaggt cttgcttggg taacttgacc
    19441 ttgattagct gttagtttat cttgctgttg atagtagatg cgatagtagc gacttggaat
    19501 gtcacttcca atacggtgtt tatcttcttg taagtcagca tagagtgttt ccaaatgagg
    19561 cccctgctct aaaatggcct gccaaaaatc ttggtctttg gccacacgtt caaaccaagg
    19621 aacaatagct tgttgaaacg ctggcttatc tagctttctc aacgtattga cacgttctac
    19681 caattcatct acccaagctg ataacgttga tacatcctta gccaggagag cttcttccac
    19741 ttcaaaagga aggtccctgt gcagatgacg attgccatta tgccaagcaa atgcctgtaa
    19801 agctaaggct aattcagcat ctcctgtata gtctgttaag gtttctagcc aagagttact
    19861 aggctgataa cggttagcat cccacagata atggctcatg tctgtcaagg tcagcttaga
    19921 taattcccaa ctaatcatag ggttagaaac aatccccttg acctgatagt ctgattggca
    19981 taaaaatggt gagcgattag caaaaggggt taaaaagaga cgttctggat ctttttgata
    20041 atcattaaca ggaatattgt cccaaataat gataggccgc tgatatactg ctgccatggt
    20101 ttcgatatct gcttgcgaga tttgactcgc tagcgtgctt gggcctgtcc agaaaaaggc
    20161 gacttcttta ggaatccttt ctgacagctc ttgcaaatag atagaatcat ggtggttgtc
    20221 gtattctgtc ggacagatca ccaattctgg cgccgcatgt tgttggttta aaaagtgatg
    20281 aacctcagtt gccaagtggg cctgggcata ggcagtcttt ttgaatcgtc tctcaacagc
    20341 atctacaatt tggtagtcaa tatcatccag caaaagccca aaatgacaaa cacctaacgc
    20401 tagaagttgt tgcaattttt gatagagcaa ctgatagtca gcttccttgg tataatcaaa
    20461 atcaagtccc ggactaatca tataccaaaa atcaatccct tcttccttgg ctactgcaag
    20521 tagctctttg aaataagtca cccaatcctc aggataaaga tcgcgccaca gttttcgctg
    20581 gtaatcatca tctttcggag catacatgta agtattcatc cttttacgtc caatgaaacg
    20641 caggcaatct aaccgctctt ctcgtgtcca cggtgttccg taaaagcctt caattatccc
    20701 ccgcatagca aaagaaggac tatgcttgac agatacaatc ggtaaataaa gtttatcccc
    20761 tttgaaagtc atcactttaa gcaaagcttc ttgggcgtaa cgaaaacctc taaggttttg
    20821 gctttcaata ataatttcct tatcttctga aaccttcagg gtaaaggcat ccttatccag
    20881 ctgacggtta aagaaatgtt taacggtcaa ctctgcttgg ccttcttcaa tccactgaaa
    20941 atactttgac gttgccaatt ctgccagtag ggctttttga ttttcaaaac ggaaaaaacg
    21001 gccaataaag cggcatgtca acggcatgtc aaagacaaga tagtctcctc ttgtcatgac
    21061 ttcttgataa tcctttaagg cgtgataaat ggtcatttct ggtctccttt acctgagcta
    21121 accagctcat gctagtaacg tcttttttag tgactacttt ctctgataaa tacttgaaca
    21181 ggtaactggc aagacaccac tgcttggtct ggctcatgaa taatctgcaa caagacttta
    21241 gctgcttctt gccccatttg gtaaaaatct tgagctgctg tgcttaattt gggactcaac
    21301 aaacgggagg cttgactatt atcaaagcct acaatagcga cttgatcagg aatatataag
    21361 cctacctgtg tgattttttg aatcagctga atagccaacc aatcgttttc cacgacaagc
    21421 ccccttttag tggctgtcgt ctccgataag gttttcacca gacggtccaa ataagcatct
    21481 gttgtttcag tctctgtctt aggaaaataa tgggatttta gagacgattc tgccattgcc
    21541 gctaagtaac ccaaataacg gtctctaacg gatgatactt ctccaaaaga gcctgttgcc
    21601 acaaaactaa tctggtcaca gccttgctca atcaagtgct gagtcaattg acaggcaccg
    21661 cctttattat ccgctacaac agacggaaac tgaaaaagat ctagagattt atccaacaag
    21721 actagaggaa tgtgatgatg gtcgcagtaa aacaggaaat caatactgtg attgacatct
    21781 tcaggataat aaataatccc agcatagtct gacagggtgt ctaaccttac tgtactggcc
    21841 aattgaacca tgagacggta acctgtttca gcaatggagg tcataattcc cttagcataa
    21901 tctcccaatt cataatcaga agtaaaagga agaatcaaca ataaatcctt attcgcttca
    21961 ggggacgtca cttgcttttc agctacgaag cttcctttcc cacgacttct cgtaatcaag
    22021 ccttcttgct ccagttctac aagcgctcgc ttagaagtaa tacggctaac cgagaactgt
    22081 tctgacaatt ctttttcagt aggtaggcgg tcaccaattt gcaaacgacc tgatgctatc
    22141 tgctctttaa tgaaatgata tatctttttg tataataatt ttgtcattct ttactcccgt
    22201 tatcagtata gcatgtcaaa aaagagttgt aaaccttttc aaaaacttat agacaaagtt
    22261 taacttatca tttataatga aaccgaagat aaatgatata tcattttgga ggatatgata
    22321 tgacacgacc aatcccaact agcgtcagcc aatttatggc caaggtggaa agcctttgtg
    22381 gagaccaaca ccctgattgg gctcttaatt tcaaaaccag ctttaccaat accttagaga
    22441 caaccctaaa aacctacgaa gatggcacta gctttctctt gacaggagat attcctgcca
    22501 tgtggttacg tgattccact gcgcaaatga aaccttacct cttcctcgcc aaggaagatg
    22561 aagaaattag aaagattatt gccggtttgg tcaaacgcca attccgctac atttgtatcg
    22621 atccctatgc caatgccttc aacgaagaag ctaacggaaa aggacaccaa acagatcgta
    22681 cccaaatgaa cccttggatt tgggaacgca aatatgagat tgattccctc tgctacccta
    22741 ttcagctagc ttacttgctc tatcgtgaaa caggaagcac cgatcagttt aatgacgatt
    22801 ttcatcgtgg ggtagaactc attcttgacc tatggacagt tgagcaagac cacgcccagt
    22861 ctccttactt gtttgaacgc gatacttggc gcaaagaaga taccctaaca catgctggaa
    22921 aaggaagtcc tgtcgcacca acagggatga cctggtcagg ctttagacca agtgatgatg
    22981 cttgtcaata tggttacctc attccttcaa atatgtttgc agtggttgtg ctttcctatc
    23041 tagaagattt atataacaac ctttttcaca atgaaccagt agctactcgt gccaagcagc
    23101 tgaaagaagc tattcaatcg ggtattgcag accatgccct tgttcaaaac agcaagggag
    23161 aaaccattta cgcttatgaa gtggatggtc tgggacagtt ttccatcatg gatgatgcta
    23221 atattccaag cctccttgct gcaccttacc ttggtttttg cgctaaagat gaccctatct
    23281 atctggcaac gcgccgcacc attctcagcc aagaaaatcc ttattattat cagggaaatg
    23341 cagcagcagg catcggttct tcccatacac cagaaaatta tatctggcat atcgcccttg
    23401 ccttacaagg attgaccgct cttgaccaag acagtaaaaa agagatgctt gatttgcttg
    23461 tggcaacaga tgctggtacc cacttaatgc acgagggttt cgatgtcaat gatccttatc
    23521 aatacactcg tgagtggttc tcttgggcta acatgatgtt ttgtgaacta cttcttgact
    23581 atcttggctt tagcattact ccttagaaag tgaggccact atggcaacta aaaaagtaca
    23641 tattatttca cacagtcact gggatcgcga gtggtacatg gcttacgaac aacaccacat
    23701 gcgtctgatt aacttaatag atgacctgtt agaagttttt caaacggatc ctgattttca
    23761 tagttttcat ttggatgggc aaaccattat cctagatgat tatttgcaag tacgccccga
    23821 acgagaacct gagattagac aagccattgc ttcgggaaaa ctccgtatcg gacctttcta
    23881 tatcttacag gacgattttt tgaccagcag tgaatccaat gtgcgcaata tgctgattgg
    23941 taaggaagat tgtgacagat ggggcgctag tgtgccactt ggttattttc ctgatacctt
    24001 tggaaatatg ggacaaacac cacagctgat gttaaaagcc ggcctacaag ctgctgcctt
    24061 tggtcgtggc attcgtccaa ctggatttaa caatcaagta gataccagtg aaaaatacag
    24121 ctcacaattc tctgaaatca gttggcaagg cccagataac agtcgtattc ttggactcct
    24181 cttcgccaac tggtacagca atggcaatga gatcccgaca acagaagctg aggcgcgtct
    24241 tttttgggat aaaaaacttg ctgacgccga acgctttgcc tcaaccaagc accttctgat
    24301 gatgaacggg tgtgatcatc aacccgtaca acttgatgtc accaaggcaa tcgccttagc
    24361 caaccaactc tatcctgatt acgaatttgt gcattcctgc tttgaagatt acttgtctga
    24421 tctcgcaaat gatttaccag ataacctttc aaccgtccaa ggagagatta ccagtcaaga
    24481 gacagatggc tggtataccc tagctaacac ggcttctgct cgcatttacc tcaaacaggc
    24541 taataccaga gtctctcgtc aactcgaaaa catcaccgaa cccttagcag caatggctta
    24601 tgaggtgaca agtacctacc ctcacgacca actgcgttac gcttggaaaa ccctcacgca
    24661 aaatcaccct catgattcta tctgtggttg tagtgttgat agtgttcatc gggaaatgat
    24721 aaaccgtttt gaaaaagcct atgaagtcgg acactattta gcaaaagaag ctgcccagca
    24781 aattgctggc gcagttaaca cgagtgactt tccaacagat agccaacctt tcctcttatt
    24841 taataccagt ggccattcca aaacaagtgt tactgagctc accctgacct ggaaaaaata
    24901 ttattttggc caacgctttc ctaaagaggt ttaccaagaa gctcaagagt atttggcagg
    24961 actctcccaa tctttccaag tcattgatgt atcaggaaac actatctcag aagcagacat
    25021 tttggacaca agcatcgcct ttgactacga tttgcccaag agaaccttcc gagaacctta
    25081 tttcgccatc aaagtgagat tacggctacc aataactctc cctgccatgt cttggaaagc
    25141 cttagcatta caactaggaa acgagacagc tccttcagca acggtcccac tctacgacga
    25201 tagtaatcag tgccttgaaa atgagtttct aaaagttatg atacaaaccg atggccgtct
    25261 aaccatcacc gataaacagt caggggtgag ctatcaagat ctcctacggt ttgaagattg
    25321 tggcgatatt ggaaatgaat acatttcccg ccaaccaact catgaccaac ctttctatgc
    25381 ggaccaaggg accaccaagc ttaacatcat tagcaacacc gctcaagttg ctgaacttga
    25441 aatccagcaa acctttgcca ttcctatctc cgcagataag ctcttacagg ctgagatgga
    25501 agctgtcatt gacatcacag aacgccaagc aagacgttca caagaaaagg ctgagctaac
    25561 cttaacaacc cttatccgta tggagaaaaa caatcctcgc ctccaattta ccacacgttt
    25621 tgataaccaa atgacaaatc atcgcttgcg cgtcctattc ccaacgcacc ttaaaacaga
    25681 ccatcatcta gctgacagta tttttgaaac tgtcaaacgt ccaaatcatc cagatgccac
    25741 cttttggaag aatccaagta acccacagca ccaagaatgc tttgtgagtc tctttgatgg
    25801 tgaaaatgga gtcactattg gtaactatgg cctcaacgaa tatgagatct taccagatac
    25861 caacaccatt gccatcactc tcttacgttc tgttggtgaa atgggcgact gggggtattt
    25921 ccctactcct gaagcccaat gcctcggcaa acacagccta tcttatagtt ttgaaagcat
    25981 cactaagcaa acacaatttg ccagctattg gcgggcacaa gaaggccaag ttcctatcct
    26041 agtcgaacaa accagccatc ataagggaag cttaagagca gagcatagct atgtcactgg
    26101 ggcagacgat caagtcgctc taacggcttt caaacgtcgc ttagcagaca atgcccttat
    26161 cacgcgcagc tacaatcttt caaacgataa aacttgtgac tttagcctaa gcctgccaaa
    26221 ctacaatgct aaggtcacta atttgttaga aaaagacagc aagcaaagca cacccagcca
    26281 acttggcaaa gctgaaattt taactctagc ttggaagaaa caataaacta ttatcattag
    26341 taatagatta acatattcaa aaagagtctg ggactattgt ctcagactct taagttgatt
    26401 ttgagtgatt attaatacct ttaacatcag tcaggcttag ctatttctat taactgggtt
    26461 aaaatgccat catgaatctg cgagctaatt gtgagatagt cataacgatt taaaatggtc
    26521 gtgacattcc ttaagtcaaa ttgccagtca tctctttttt tcaaagccgg tgtagcaaac
    26581 acgctttgac tatcattttg tccttgttta gtactgcttt gaatgactat gacaagttta
    26641 tggttttgat ccagaaatgc tattgataag caaggttgct ttgtctccaa actcatttct
    26701 atagcattat ctaacaaaaa tgacaatagc ttagtgaaat ctactacttc tagaaaaaac
    26761 gttgcaatac aattaggaat cgatacctca accttaagct tcttattttt tgcctcaatt
    26821 aatttagcta aaacaatatg tctgattgct tcaacttgta atttatctaa acaagttaaa
    26881 tcaagtctgt catctttagc attattcttt tcagcattca agataccttc ataagtaagt
    26941 ctcacaatag agatatcatt ttgttcgatt ccaatcttaa gacgactgga cacggtagtt
    27001 aatagctgcc taagctcttg aatatcttga tacagcaatc caaggtgctg actataatta
    27061 atcagctcac gatattcttt ttgttgcaca attgcctcaa tagagttttg ttttacataa
    27121 cgctctaaat gaattaataa agtaagaaac aaaatgaaat aaaagagcac tatgattgaa
    27181 cgagcagtcg ttgtagcaag tcctgataaa gaaggcgaca tcacatctat cccttggaca
    27241 cacaagtaat atagagccat cgaagagtta gtaaacacta atagtttact agctctatgt
    27301 gattgaaaat attggcgcca ggttgaaaaa tcgaaatgaa aaacttgaat gcagcgataa
    27361 atcaaaaaaa tactaaagca agtaatgctg taaatcagta gtgtattatg tttaacaact
    27421 gaataaggca taccaaacaa tggcataaca aaaaatacaa taaatcgctt aaccaaacta
    27481 atagacacta gtggaaaaaa ggcataaaac attaaaaact tagcttttat tgatttacca
    27541 tagtataggg ctataagcgc aaagaaaaga aactgagcca acatcacaac atcaagaatg
    27601 aaatggtctg gaaagaaagc ccaaaaaact acttgaaaca aaaccatact aagtgataaa
    27661 taaaaaatat ttttcaggcg aagcggaatt gcacttacaa agctaaaaag ctttaac
//
LOCUS       DATXQK010000020        26108 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.020, whole genome shotgun sequence.
ACCESSION   DATXQK010000020 DATXQK010000000
VERSION     DATXQK010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26108
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.020"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            48..266
                     /locus_tag="VC394_000944"
     CDS             48..266
                     /locus_tag="VC394_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP5994422.1"
                     /translation="MYPRIRNLREDNDFTQKFVANLLSFSHANYAKIERGEVALMADV
                     LVQFSKLYNVSIDYLLGQTDYPYRFHNK"
     gene            complement(337..480)
                     /locus_tag="VC394_000945"
     CDS             complement(337..480)
                     /locus_tag="VC394_000945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994423.1"
                     /translation="MGISPTGLLRWQPERSEVSLSLFVVMADREPLYFIANNYRSYHG
                     VSH"
     gene            567..787
                     /locus_tag="VC394_000946"
                     /pseudo
     CDS             567..787
                     /locus_tag="VC394_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
     gene            1489..1872
                     /locus_tag="VC394_000947"
     CDS             1489..1872
                     /locus_tag="VC394_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEP5994424.1"
                     /translation="MKVHTTLEKDENGNWYRKDQTKTPAGERLIELDDVTIVVLENWR
                     RNQVVNTDTDFIISRFGEPFCKSTICRVIKHKAQEVGVPVITGKGLRHSHASYLINVL
                     KKDILYVAKCMGHADKSTTLNTYSH"
     gene            2135..3370
                     /locus_tag="VC394_000948"
     CDS             2135..3370
                     /locus_tag="VC394_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEP5994425.1"
                     /translation="MIKRWVILFFMVLTCSGFGKTVLAADFQVGAEHAIVVEADSGRV
                     LYEKDAKTPDAIASLTKLVTAYLVLDKVKSGQLQLSDQVNLSDYAFELTKDRSLSNVP
                     FDKKTYSVQDLLTATLVASSNSAAIALAEKVAGSELHFVNQMREQLSHWGITSGKILN
                     ASGLPNEVLKDNRYPGSALEEENTLSAQDVAVVTMHLLEDFPEILEITKQTEVNFAGN
                     SIKSFNQLLPGMAKGRAGVDGLKTGTTDLAGHCLVVTSIENGMRVITVILNADGSDKN
                     QNTRFEQANRLLDYVDRTYCRRKILKKGSLVLERSLPIQDGQVKELPVSVAEDVTIIL
                     QQGEQVPKPKQFMISETSLLAPITKGEVVAYLTSPRITDQSVRYLKEPKRIPLKASQS
                     LKKASDLQLWWRDFLEKRR"
     gene            complement(3486..4448)
                     /locus_tag="VC394_000949"
     CDS             complement(3486..4448)
                     /locus_tag="VC394_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEP5994426.1"
                     /translation="MNNRHKRRQKRKAFTLINLILLFACILGLTLIFYLYQGSNKFGL
                     SDVSQFVPKISLFHHKKTAKKETTKLKKTHFDSSKSQKKAHSKLTWTKQETPVKIPIL
                     MYHAIHVMFPEETANANLIVNPDLFDQQLQKMKDEGYYFLSPEEVYRALSNNELPAKK
                     VVWLTFDDSMIDFYNVAYPILKKYDAKATNNVITGLTEIGSAANLTLKQMKEMKQFGM
                     SFQDHTVNHPDLEQASPDVQTTEMKDSKDYLDKQLNQNTIAIAYPSGRYNDTTLQIAA
                     RLNYKLGVTTNEGIASAANGLLSLNRIRILPNMSPENLLQTMEP"
     gene            4780..6057
                     /locus_tag="VC394_000950"
     CDS             4780..6057
                     /locus_tag="VC394_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEP5994427.1"
                     /translation="MTYEETLEWIHDHLVFGIKPGLKRMLWVLGQLGNPQKNVKGVHI
                     VGTNGKGSTVNHLQHIFTTAGYEVGTFTSPYIMDFKERISINGRMISEKDLVIAANRI
                     RPLTERLVQETDFGEVTEFEVITLIMFLYFGDMHPVDIAIIEAGLGGLYDSTNVFQAM
                     VVVCPSIGLDHQAILGETYADIAAQKAGVLEGGETLVFAVENPSAREVFLTKAEQVGA
                     SIWEWQEQFQMAENASGYRFTSPLGVISDIHIAMPGHHQVSNAALAIMTCLTLKDRYP
                     RLTSDHIREGLANSLWLGRTELLAPNLMIDGAHNNESVAALVAVLKNNYNDKKLHILF
                     GAIDTKPIADMLVALEQIGDLQVTGFHYPNAYPLEKYPERFGRVADFKDFLALRKHAK
                     ADDFFVITGSLYFISEIRQYWKKHIEKNVLLTH"
     gene            6092..6676
                     /gene="folE"
                     /locus_tag="VC394_000951"
     CDS             6092..6676
                     /gene="folE"
                     /locus_tag="VC394_000951"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEP5994428.1"
                     /translation="MKRERLMSINKEKAEAAIYQFLEAIGENPNREGLLDTPKRVAKM
                     YAEMFLGLGKDPKEEFTAVFKEQHEDVVIVKDISFYSICEHHLVPFYGKAHIAYLPSD
                     GRVTGLSKLARAVEVASKRPQLQERLTSQIADALVEALNPKGTLVMVEAEHMCMTMRG
                     IKKPGSKTITTTARGLYKESRAERQEVISLMTKD"
     gene            6685..7485
                     /gene="folP"
                     /locus_tag="VC394_000952"
     CDS             6685..7485
                     /gene="folP"
                     /locus_tag="VC394_000952"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEP5994429.1"
                     /translation="MKIGKFVIEGNAAIMGILNVTPDSFSDGGSYTTVQKALDHVEQM
                     IADGAKIIDVGGESTRPGCQFVSATDEIDRVVPVIKAIKENYDILISIDTYKTETARA
                     ALEAGADILNDVWAGLYDGQMFALAAEYDAPIILMHNQDEEVYQEVTQDVCDFLGNRA
                     QAALDAGVPKNNIWVDPGFGFAKSVQQNTELLKGLDRVCHLGYPVLFGISRKRVVDAL
                     LGGNTKAKERDGATAALSAYALGKGCQIVRVHDVKANQDIVAVLSQLM"
     gene            7492..7851
                     /gene="folB"
                     /locus_tag="VC394_000953"
     CDS             7492..7851
                     /gene="folB"
                     /locus_tag="VC394_000953"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEP5994430.1"
                     /translation="MDKIVLEGCRFYGYHGAFKEEQTLGQIFLVDLELSVDLQAASLS
                     DQLTDTVHYGMVFDSVRQLVEGEKFILIERLAGAICEQLFNEFPPIEAIKVAIKKENP
                     PIAGHYKAVGIELERQR"
     gene            7848..8348
                     /gene="folK"
                     /locus_tag="VC394_000954"
     CDS             7848..8348
                     /gene="folK"
                     /locus_tag="VC394_000954"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEP5994431.1"
                     /translation="MTIVYLSLGTNMGDRAAYLQKALEALADLPQTRLLAQSSIYETT
                     AWGKTGQADFLNMACQLDTQLTAADFLKETQAIEQSLGRVRHEKWGSRTIDIDILLFG
                     EEFYDTKELKVPHPYMTERAFVLIPLLELQPDLKLPPNHKFLRDYLAALDQSDITLFS
                     AQQTEF"
     gene            8498..9385
                     /gene="murB"
                     /locus_tag="VC394_000955"
     CDS             8498..9385
                     /gene="murB"
                     /locus_tag="VC394_000955"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEP5994432.1"
                     /translation="MITELHGIDIRENEPLKHYTYTKVGGPADFLAFPRNHYELSRIV
                     AYANKENMPWLVLGNASNLIVRDGGIRGFVIMFDKLNAVHLNGYTLEAEAGANLIETT
                     KIAKFHSLTGFEFACGIPGSIGGAVFMNAGAYGGEISHIFLSAKVLTSSGEIKTISAR
                     DMAFGYRHSAIQETGDIVISAKFALKPGNYDTISQEMNRLNHLRQLKQPLEFPSCGSV
                     FKRPPGHFAGQLIMEANLKGHRIGGVEVSEKHAGFMINVVDGTAKDYEDLIAYVIETV
                     ENHSGVRLEPEVRIIGENL"
     gene            9431..10585
                     /locus_tag="VC394_000956"
     CDS             9431..10585
                     /locus_tag="VC394_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5994433.1"
                     /translation="MTKPIITFNNVSKTFEDSGTQVLKNINFDLEEGKFYTLLGASGS
                     GKSTILNIMAGLLDASSGDIYLDGERINDLPINKRDIHTVFQNYALFPHMTVFENVAF
                     ALKLKKVDKKEIAKRVKETLKMVQLEGYENRSIQKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTEMQYELRELQQRLGITFVFVTHDQEEALAMSDWVFVMNEGEIVQ
                     SGTPVDIYDEPINHFVANFIGESNIINGTMIEDYLVSFNGKEFESVDGGMRPNEPVEV
                     VIRPEDLQITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIEGEVIG
                     LDFTPEDLHIMRLNETEEEFDARIEEYVEMDEPEDGLINAIEEERNEENL"
     gene            10569..11363
                     /locus_tag="VC394_000957"
     CDS             10569..11363
                     /locus_tag="VC394_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP5994434.1"
                     /translation="MKKTSSLFSIPYFLWILFFVVAPVTLLFYKSFFDIEGRVTLANY
                     ETFFSSWTYLRMSVNSILYAGIITLVTLLISYPTALFLTRLKHKQLWLMLIILPTWVN
                     LLLKAYAFMGIFGQQGGINSFLTFMGIGPQQILFTDFSFIFVASYIELPFMMLPIFNA
                     LDDIDHNVINASRDLGASEFQAFSKVIFPLSLNGVRAGVQSVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLTTQNWGMGSTIGVVLILTMVAIMWLTKEKSK"
     gene            11360..12136
                     /locus_tag="VC394_000958"
     CDS             11360..12136
                     /locus_tag="VC394_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP5994435.1"
                     /translation="MKKFANLYLASVFVLLYIPIFYLIFYSFNKGGDMNGFTGFTLEH
                     YQTMFEDSRLMTILLQTFVLAFSSALLATIIGTFGAIFIHHVRGKYQNAMLSANNVLM
                     VSPDVMIGASFLILFTSLKFQLGMSSVLLSHIAFSIPIVVLMVLPRLKEMNQDMVNAA
                     YDLGANYFQMLKEVMLPYLTPGIIAGYFMAFTYSLDDFAVTFFLTGNGFTTLSVEIYS
                     RARQGISLDINALSTIVFFFSILLVIGYYYMSQDKEEKHA"
     gene            12129..13202
                     /locus_tag="VC394_000959"
     CDS             12129..13202
                     /locus_tag="VC394_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEP5994436.1"
                     /translation="MRKLYSFLAGVLGVIVILTSLSFILQKKSGSGSQSDKLVIYNWG
                     DYIDPALLKKFTKETGIEVQYETFDSNEAMYTKIKQGGTTYDIAVPSDYTIDKMIKEN
                     LLNKLDKSKLVGMDNIGKEFLGKSFDPQNDYSLPYFWGTVGIVYNDQLVDKAPMHWED
                     LWRPEYKNSIMLIDGAREMLGVGLTTFGYSVNSKNLEQLQAAERKLQQLTPNVKAIVA
                     DEMKGYMIQGDAAIGITFSGEASEMLDSNEHLHYIVPSEGSNLWFDNLVLPKTMKHEK
                     EAYAFLNFINRPENAAQNAAYIGYATPNKKAKALLPDEIKNDPAFYPTDDIIKKLEVY
                     DNLGSRWLGIYNDLYLQFKMYRK"
     gene            complement(13257..13922)
                     /locus_tag="VC394_000960"
     CDS             complement(13257..13922)
                     /locus_tag="VC394_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEP5994437.1"
                     /translation="MNVLIIEDDPMVDFIHRNYLEKLNLFDRIISSDSMKAVQSILTD
                     YAIDLILLDIHITDGNGIQFLEKWRAQHIPCEVIIISAANDGNIIRDGFHLGIIDYLI
                     KPFTFERFQESIQQFVTHREHLANQQLEQAQIDQLKCLTSKKDTKNKQLLEKGLSEST
                     FQWIIENIKAFDQPFTIQELASACHLSHVSVRKYIAYLEENKQLNSQQIFTKVGRPYR
                     VYW"
     gene            complement(13903..15444)
                     /locus_tag="VC394_000961"
     CDS             complement(13903..15444)
                     /locus_tag="VC394_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEP5994438.1"
                     /translation="MKKPLRLWASLSLILVSMIVVTTSLFYGIMLHDTHQSIKNQETH
                     LLTSTGKMLASHQAIKELLLNNQPNAKTTAYTNSIASIYNLDYVVVMNMKGIRLTRPN
                     PKNIGKPFQGGDEEAVLAGKKVISTAKGTLGKSLRYLVPVFDGDKQIGAIAVGIKLTT
                     LNDVALTSKRNYTLSLFLCLLISLLVTSFISFRLKRQLHQLEPSEIYQLFEERNAMLD
                     QIEAAVFVVDKTGILQLCNQAGQELIARKCQLGKPTGNSFNHLFPDFPKLSLQEGHEQ
                     LFRYGEEDYLLAISPICVKNDHRGHIIFMREAVKAIDTLDQLAYTTAYASALQAQTHK
                     FMNQLHVIYGLVDIAYYDQLKIYLDSILEPENEILTSLSVLVKEPLLASFLIGEQEKY
                     QELNVHLKIDVLSEIPHSVTKNQLNNGLMIYRFIHTNLLTTLRPKSLVLSIQHDQNHL
                     ISHYTLTDNWIDLERVQPIFDLPYFQQLLTDTNSQFHQHQTVTELQFSVTTPYVGWQH
                     ERFNH"
     gene            15605..16936
                     /locus_tag="VC394_000962"
     CDS             15605..16936
                     /locus_tag="VC394_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HEP5994439.1"
                     /translation="METISKKMPQKDLSEHSKAWQNRRIGSVPLPVYLVLATLILITG
                     WLQQLPVNMLGGFAVILTLGWLLGTIGATIPGLKHFGGPAILSLLVPSILVFFNLLNP
                     NVLEATNVLMKQANFLYFYIACLVCGSILGMNRKILIQGLFRMIIPMLLGMVCAMGVG
                     TLVGVILGLDWQHTLFYVVTPVLAGGIGEGILPLSLGYSAITGVGSEQLVAQLIPATI
                     IGNFFAILCTALLNRFGEKHPSYSGQGQLVKIGHSEDMSDALKDNSGALDVKLMGAGV
                     LTACSLFIAGGLLQHLTGFPGPVLMIILAAFLKYLNVIPQETQNGAKQLYKFISSNFT
                     FPLMAGLGLLYIPLKEVVATLSWQYFIVVISVVLTVVSVGFFVSRFLNMNPVEAAIIS
                     ACQSGMGGTGDVAILSTADRMNLMPFAQVATRLGGAITVITMTAILRIIFS"
     gene            16968..18134
                     /locus_tag="VC394_000963"
     CDS             16968..18134
                     /locus_tag="VC394_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEP5994440.1"
                     /translation="MKNQLGQLALEQAKTFGGKLEVQPKVDITNKHDLSIAYTPGVAG
                     VSSAIAKDKTLAYDLTTKKNTVAVISDGTAVLGLGDIGPEAAMPVMEGKAALFKAFAG
                     VDAIPIVLDTKDTEEIISIVKALAPTFGGINLEDISAPRCFEIEQRLIKECHIPVFHD
                     DQHGTAIVVLAAIFNSLKLLQKSLDEVSIVVNGGGSAGLSITRKLLAAGATKVTVVDK
                     FGIINEQEADQLAPHHLDIAKVTNREFKSGTLEDALEGADIFIGVSAPGVLKAEWISK
                     MAARPVIFAMANPIPEIYPDEALEAGAYIVGTGRSDFPNQINNVLAFPGIFRGALDAR
                     AKTITVEMQIAAAKGIASLVPGDALSTTNIIPDAFKEGVAEIVAKSVRSVVLKS"
     gene            complement(18216..19154)
                     /locus_tag="VC394_000964"
     CDS             complement(18216..19154)
                     /locus_tag="VC394_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HEP5994441.1"
                     /translation="MKAATYLSTGNLQLIDKPKPVIIKPTYRPVGKNHSICGTDLHIL
                     GGDVPACKEGTILGPEGIGIVKEIGDAVTNFKIGDKVIISCVTSCHTCYYCKRGLSSH
                     CEDGGWILGHLINGTQAEYVHIPHADGSLYHAPDTIDDEALVMLSDILPTSYEIGVLP
                     SHVKPGDNVCIVGAGPVGLAALLTVQLFSPANIIMVDLSQNRLEAAKTFGATHTICSG
                     SSEEVKAIIDDITNGRGVDISMECVGYPATFDICQKIISVGGHIANVGVHGKPVDFNL
                     DELWIKNITLNTGLVNANTTEMLLNVLKVIIDNDIS"
     gene            19390..20121
                     /gene="aphA"
                     /locus_tag="VC394_000965"
     CDS             19390..20121
                     /gene="aphA"
                     /locus_tag="VC394_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HEP5994442.1"
                     /translation="MKKEFTSILFTVSFCGIIALPVEASGPKVPYTQEGITAISNQAT
                     VKLISIADIASSLEGQKPITVSFDIDDTLLFTSQYFQYGKEYITPGSFDFLHKQKFWD
                     LVAKRGDQDSIPKEYAKQLIAMHQKRGDKIVFITGRTRGSMYKKGEIDKTAKSLAKDF
                     KLDKPIAINYTGDKAVKPYQYDKTYYIKKNGSQIHYGDSDEDINAAKEAGARPIRILR
                     APNSTNLPLPKAGGYGEEVLENSAY"
     gene            20299..21831
                     /locus_tag="VC394_000966"
     CDS             20299..21831
                     /locus_tag="VC394_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HEP5994443.1"
                     /translation="MENHKNEFTFSNKSIIAYVWRGVMVGIIAGVIVSLFRLLIEVTA
                     DWVIEWYRYAHINSLLLLPILSVSLLAVLFVGFLVKSDSDIKGSGIPHVEGELKGLMS
                     PDWWSVLWKKFLGGIMAISMGFMLGREGPSIQLGAMSAKGLAKFLKSSRLEKRVLIAS
                     GAAAGLSAAFNAPIAGLLFVVEEIYHHFSRLIWITALVASLVANFISLNIFGLKPVLA
                     MSEAMPFLGLNQYWLLLLLGLFLGCLGYLYEIVILNFNKLYVILGNWLHLPAYFYGII
                     MVFLILPIGYYLPQLLGGGHGLILSLSNQQLPLMTIFFYFIIRFIVSMFSYGSGLPGG
                     IFLPILTLGALAGLLFGQIASQLGLLNQSFLSLFLILGMAGYFAAISKAPLTGMILVT
                     EMVGDLKPLMAIAVVTFVSYLVMDLLNGQPIYEAMLDKMAMKHPTNLVEPTLIELTVG
                     DKIAGKYVKELKLPENVLITTQIHHQKSQVVSGNTRLLSGATIFLVVNEADTGFVREV
                     LM"
     gene            21994..22608
                     /locus_tag="VC394_000967"
     CDS             21994..22608
                     /locus_tag="VC394_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HEP5994444.1"
                     /translation="MLEIVSEELRHYQEQKLIEYRNKNQLAPKGGIVFAGDSLIEFFP
                     LKKAFGSCLPIINRGIAGIDSQWLLRHFSVQITDLEPKHIFLLIGCNDIGFGYDKCHI
                     VKTIVELISQIRSHCVYSQIYLLSLLPVSNNPRYQKTVKIRTNAMIDAINKDLAMIPT
                     IEFINLNTCLKDEKGGLSDENTLDGLHLNFPAYAKLAEIIQAYI"
     gene            22767..23915
                     /locus_tag="VC394_000968"
     CDS             22767..23915
                     /locus_tag="VC394_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEP5994445.1"
                     /translation="MIIKGVTMKFEKKQVFYLVLTFILCYGILANWRNGTAIVTTIYK
                     TSLPFFYGAAGAYIVNIVMSAYEKVYVYIFKDWSHVLKVKRGICLLLAYLTFFILITW
                     IISIVIPDLITSISTLTKFDTITIQEVVNNLEHNKLLARTIQYIGGDGKLTETIANYS
                     QQLLKQFLTVLTNILTSVTVIASAIINLFISFVFSLYVLASKEDLCRQGNTLVDTYTG
                     KYAKRIHYLLELLHQRFHGFFVSQTLEAMILGSLTASGMFILRLPFAGTIGVLVAFTA
                     LIPVIGASIGAAIGFILIMTQSMSQAIIFIIFLIILQQIEGNFIYPKVVGGSIGLPAM
                     WVLMAITIGASLKGIVGMIIAVPLAATLYQVIKDNIQKRQAIQKKQVS"
     gene            23964..24644
                     /gene="radC"
                     /locus_tag="VC394_000969"
     CDS             23964..24644
                     /gene="radC"
                     /locus_tag="VC394_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEP5994446.1"
                     /translation="MYSIKCDDNKAMPRERLMRLGAESLSNQELLAILLRTGNKEKHV
                     LELSSYLLSHLDSLADFKKMSLQELQHLAGIGKVKAIEIKAMIELVSRILATDKTLTD
                     SVLTSVQVAEKMMAALGDKKQEHLVVLYLDNQNRIFEEKTIFIGTVRRSLAEPREILY
                     YACKNMATSLIVIHNHPSGNIEPSSNDYCFTEKIKRSCEDLGIICLDHIIVSYKDYYS
                     FREKSTLF"
     gene            complement(24646..25341)
                     /locus_tag="VC394_000970"
     CDS             complement(24646..25341)
                     /locus_tag="VC394_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEP5994447.1"
                     /translation="MTKPIIGITANQRLNMALDNLPWSYAPTGFVQAVTQSGGLPLLL
                     PIGDEAAAKTYVSMVDKIILIGGQNVDPKYYQEEKAAFDDDFSPERDTFELAIIKEAI
                     TLKKPILGICRGTQLMNVALGGNLNQHIDSHWQEAPSDFLSHEMIIEPDSILYPIYGH
                     KTLINSFHRQSLKTIAKDLKVIARDPRDGTIEAVISTNDAIPFLGVQWHPELLQGVRD
                     EDLQLFRLFVNDF"
     gene            complement(25351..25995)
                     /locus_tag="VC394_000971"
     CDS             complement(25351..25995)
                     /locus_tag="VC394_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEP5994448.1"
                     /translation="MVIDKSIPKATAKRLSLYYRIFKRFHADQVEKASSKQIADAMGI
                     DSATVRRDFSYFGELGRRGFGYDVTKLMNFFADLLNDHSTTNVILVGCGNIGRALLHY
                     RFHDRNKMQIAMGFDTDDNALVGTKTADNIPVHGISSVKERIANTDIETAILTVPSIH
                     AQEVTDQLIEAGIKGILSFAPVHLQVPKGVIVQSVDLTSELQTLLYFMNQNHLD"
BASE COUNT         7956 a         4371 c         5430 g         8351 t
ORIGIN      
        1 cattttttaa tcaaatctcc tttatactga tattcggaga atgttagatg tatccgcgta
       61 ttaggaattt gagagaagat aacgatttca ctcaaaagtt tgttgcaaat ttactttcct
      121 tctctcatgc aaattatgca aaaattgaga gaggagaggt tgccttaatg gcagatgttc
      181 ttgtgcagtt ttctaaactc tataacgtca gtattgatta tttattaggg caaacagatt
      241 atccgtatcg ttttcataat aaataaaagt ctcgccttga tttgttcatt gacaactgaa
      301 taaaccgagg tgggactttc ttgtttgtcg agcaatttag tgagatacgc cgtgatagga
      361 gcgataatta ttagctataa aatagagtgg ttctctatct gccatgacca caaataggga
      421 caatgatact tctgaacgtt caggctgcca gcgtaaaagg ccagtgggtg agattcccat
      481 gagtgattta accagtcata cccacggagg ttgtaatttt tttgaaagga gtggaggtgt
      541 gaaaatagta acatataaaa agcttgatga atattggttt tctcgaacat actgcaagaa
      601 atattattag agaagcaaaa agaatagcta taaaaaagtt gaagaagctc gcgaacttga
      661 acaaaatgcg gtacaattaa gtaaatcgcc ctttgacaat agaagacttg gtattgctcc
      721 taaagatatt gtagaagagt tagtaggtgt tctactctct gaagaaagtt tagaaagtga
      781 gaattaaaga tgactataaa aaatcaaaaa aagtacaaag gtgtttattg tgataagaat
      841 gggaagatat tttatcaagc tgatcttggt gtagatccag taacaggtaa acgagttcaa
      901 aagaaagcca gaaagaatta gtatggaaag ccatttggta caatgaaaga agcgtatgat
      961 gaactggttc ggattaggta tgagttcagt aataatctta gtattgaaaa tgacaatata
     1021 tcgtttgaaa actatatgaa tactatttac ctaagagcat ataagcaaaa ggttcaggct
     1081 gtaacttatg aaatagcctt accacatcat aagttattta ttcagtattt tggttctaaa
     1141 cttttaaagg gaataacagc ttgagattgt gaatctttta gtttacatat tattgaaaat
     1201 tatgcaaaga atttatggtc gcgattcaaa gcttgcatgg ggtatgctga aaggttagac
     1261 tatatttcaa aagtgccatg taaggctttg gataatccta aagggaaaca tcccgacact
     1321 ccattttgga cttatattga gtttcagaat tttataaaat cgtttgattt gcaagattat
     1381 gaagagctac aaagatttac aactatttgg ctatattata tgacaggtgt tcgtgtaagt
     1441 gaaggtttat cattatgatg ggaaaatatc gatttcgaac aaaaattctt gaaagttcat
     1501 acaaccttgg aaaaggatga aaatggaaat tggtatcgta aagaccaaac aaaaacgcca
     1561 gcaggagaac gtcttattga gttagatgat gtgacaatag tagtattgga aaattggaga
     1621 agaaatcaag tagtcaatac tgacacagat ttcattattt cacggtttgg tgaaccattt
     1681 tgtaaatcaa cgatttgtcg agttattaag cacaaagctc aagaagtagg agtaccagtg
     1741 ataacgggaa aaggcttgcg acatagtcat gcttcctatc ttattaatgt tttgaaaaaa
     1801 gacattctgt acgttgcaaa gtgtatggga catgctgata agtctacaac tttgaataca
     1861 tatagtcact gatttaacgc attagataaa acagtatctg aagaaataac ccaaaatgta
     1921 ataagttcag gattagattc cattttatac caaaattcct gccaaacaac taattgatat
     1981 aacgaagctt attatatcgc ccatataaca ggatgtgtta ttgttacagg gtacttgctt
     2041 tatgagcttg tttaagctac taaaggtggg tgccactata cttccttcca aatttctgag
     2101 aatttttgct ataatagtaa ctatgaaaga tataatgata aaaaggtggg ttatcctttt
     2161 ttttatggta ttaacttgtt cagggtttgg taaaacagtt ctcgcagctg attttcaagt
     2221 gggagcagaa catgcgattg tcgtagaggc agactctggg agagttctgt atgagaaaga
     2281 cgctaagacg ccagatgcta ttgcctcttt gaccaaattg gtgacggctt atctggtttt
     2341 agataaggtt aaatcaggcc agctgcaatt atcagatcaa gttaatctct cagattatgc
     2401 ctttgagtta acaaaagata ggtctttaag taatgtacct tttgacaaaa aaacttattc
     2461 agttcaagac ctgttgacag ctactttggt agcaagctca aatagcgctg ctattgcgtt
     2521 agccgaaaaa gtggcgggct cagagcttca ttttgtaaat caaatgagag aacagctatc
     2581 tcattggggg attacttctg gtaaaatcct caacgcctca ggtctgccaa atgaggtgtt
     2641 aaaggacaat cgttatcctg gttctgcgtt ggaagaagaa aataccttga gtgctcaaga
     2701 tgttgcagtt gtgacgatgc atttgttaga agattttcct gagattttag agatcaccaa
     2761 acaaacagaa gttaattttg ctggcaattc tattaaaagt tttaatcaac tcttaccagg
     2821 tatggctaag ggtagagcag gagtggatgg tttaaagact gggacaacag acttggcagg
     2881 tcattgtctt gtggtaactt ctattgaaaa tggtatgcga gtgattaccg ttattctaaa
     2941 tgccgatgga tctgataaaa accaaaacac gcgatttgag caagcaaatc gtttgttgga
     3001 ctatgtggat agaacttatt gtcgtcgtaa gattcttaaa aaaggaagtc ttgtcttgga
     3061 gcgatcgctc cctattcaag atggccaagt aaaagaacta ccggtatctg ttgccgaaga
     3121 tgtaaccatc attttgcaac agggtgaaca agtccccaaa cctaaacaat ttatgatatc
     3181 agagacttct ttactagcac cgattactaa aggtgaagtg gttgcttatt tgacgtctcc
     3241 acgaataaca gatcagtcag tgaggtattt aaaggaacct aagcgtattc ctcttaaagc
     3301 tagccaatct cttaaaaaag cctctgactt acagctgtgg tggcgagatt ttcttgaaaa
     3361 aagacgttaa agataagaat aaaataattt taaaacaatc aaaaaagaca gttatgaaag
     3421 aattttttca taactgtctt tttagtttac agtgagtatg atttggaatg ataaaaaaat
     3481 tgatgttatg gttccattgt ttgtaaaaga ttttccggac tcatgtttgg gaggatacga
     3541 atccggttaa gagaaagaag tccattagcc gcgctagcta ttccttcgtt agtggtaaca
     3601 cctaatttat agttaagtct agctgcaatt tgtaaggtag tgtcattata tctaccagat
     3661 ggataagcaa tggcaatagt gttttggttt aattgcttat caagatagtc ctttgaatct
     3721 ttcatttcag ttgtttgaac atcaggacta gcctgctcta aatcaggatg attcactgta
     3781 tgatcttgga aagacatacc gaattgtttc atttctttca tctgcttaag agtcagattg
     3841 gcagcgcttc ctatttcggt tagtcctgta ataacattat tggtagcttt agcatcatat
     3901 ttttttaaga ttgggtaagc aacgttgtag aaatcaatca tactgtcatc aaatgttagc
     3961 catacaactt ttttagcagg caattcatta tttgaaagag cgcggtagac ctcttcagga
     4021 ctcaaaaaat agtagccctc gtctttcatt ttttgaagtt gttgatcaaa gagatctggg
     4081 ttgacaatca aattagcatt ggccgtctct tcagggaaca ttacatgaat agcatgatac
     4141 attaaaatgg ggatcttgac aggtgtttct tgcttggtcc aagttaattt agaatgggcc
     4201 tttttttgag attttgagga atcgaaatga gtcttcttaa gttttgtcgt ttcttttttt
     4261 gccgtctttt tgtgatgaaa aagacttatt ttaggtacaa attgagagac atctgacaag
     4321 ccgaatttgt tagacccttg gtataaatag aaaatgaggg tcaaaccaag aatgcaggca
     4381 aataatagta taaggttaat gagagtgaag gcttttcttt tttggcgccg tttatgtcta
     4441 ttgttcatta cttgctcctt tttttgatat aagtacctct attgtaacat tttttagaga
     4501 aaagaaggct tgaagtcagc cttttttctt gttttttgga agaacgtgta ttaaggagta
     4561 aaaaaattgt tgtcacagat aacactttag gaggtagcaa agactatgtg gggctggtat
     4621 tcttatcacg taaaaaaagc aatgaagata aaagtgtagt gtactttaga agttatttga
     4681 gtgtttggtg aggataataa aaaaaagggt actactctag gttatctgct gtcacttttt
     4741 tttatatatg gtatactaaa gatagatgtg aggtatgaaa tgacttatga agagactcta
     4801 gaatggattc atgatcactt agttttcggc atcaagccag gcttaaaacg tatgctttgg
     4861 gttcttggac agttaggaaa tccccaaaaa aatgtcaagg gagtgcatat cgttggaact
     4921 aacggaaaag gttccacggt caatcattta caacacatct ttacgacagc tggttatgaa
     4981 gtggggacct ttacctctcc ttatattatg gatttcaaag aacgtattag cataaatggt
     5041 cgaatgattt cagaaaaaga tttggttatt gctgctaatc gtattcgtcc tttgacagag
     5101 cgtctagttc aagagactga ttttggagag gtaactgaat ttgaagtgat tactttgatc
     5161 atgttcctct attttggaga catgcatcct gtagatattg ctattattga agcaggcctt
     5221 ggcggtcttt atgattcgac caatgttttt caagcaatgg ttgtggtctg tccctcaatt
     5281 ggtttagatc accaagctat tttgggagag acttacgctg atattgccgc ccaaaaagct
     5341 ggtgttttag aagggggcga gacccttgtt tttgccgttg aaaacccgtc agctcgagag
     5401 gtttttttaa caaaagctga gcaggttggt gcttcaatct gggaatggca agagcagttt
     5461 caaatggctg agaacgcatc tggctatcgt tttactagtc ctttaggagt tatttcagat
     5521 attcacatcg ctatgccagg acaccaccaa gtgtctaatg cagctttagc tattatgact
     5581 tgcttaacct tgaaagaccg ctatcctagg ctaacttcag atcatataag ggaaggtctg
     5641 gcaaatagtt tgtggctagg ccgtaccgaa ttattagcac caaatctaat gattgatggg
     5701 gctcataaca atgaaagcgt tgctgctttg gtagccgtct taaaaaataa ctacaatgat
     5761 aaaaagcttc atattttgtt tggtgcgatc gacaccaagc ctattgcgga tatgttggtt
     5821 gctcttgagc aaataggtga cttgcaggtt actggctttc attaccctaa cgcctatcca
     5881 ttggaaaaat acccagaacg ttttggtagg gttgctgatt tcaaagattt cttggccctg
     5941 cgtaagcatg ccaaagcaga tgactttttc gtgattacag ggtcgctata ttttatttca
     6001 gaaattagac agtattggaa aaaacatatt gaaaaaaacg ttcttttaac ccattaaaaa
     6061 agagaaaatg aaaaggaaaa ggaaaaaact aatgaaaagg gaaagactaa tgtctattaa
     6121 taaggaaaaa gcagaagcag ccatttatca gtttttagag gctattggtg aaaatccaaa
     6181 tcgggaaggt cttcttgaca cgcctaaacg ggttgctaaa atgtatgcgg agatgttttt
     6241 gggactggga aaagatccta aagaggagtt tacggctgtc tttaaagaac aacatgagga
     6301 tgtggtgatt gtcaaagaca ttagttttta ttctatatgt gaacaccact tagttccttt
     6361 ttatggcaag gctcatatcg cttacttgcc aagtgatggt cgagtaacag gtttgagtaa
     6421 attagcgcga gcagttgaag tagctagtaa acgacctcaa ctccaagagc gtttgacctc
     6481 tcaaatagcg gatgctttag ttgaagccct caatcctaaa gggactttag ttatggtaga
     6541 agcagagcac atgtgcatga ccatgcgagg catcaaaaag ccaggtagta aaaccattac
     6601 caccactgcc agaggcttat ataaggaaag tcgtgctgag aggcaagaag ttatttctct
     6661 aatgacaaaa gattaggaga acttatgaaa attggaaagt ttgtgattga gggcaatgcg
     6721 gctatcatgg ggattttaaa tgtgactcca gattcttttt cagatggggg gtcttacact
     6781 actgtgcaaa aagcattaga tcacgttgag caaatgattg ctgatggggc taaaatcatc
     6841 gacgttggtg gagaatcaac acgtccaggt tgccaatttg taagcgctac cgatgaaatt
     6901 gaccgggtgg ttcctgtgat caaggccatc aaagaaaact atgatattct aatcagcatt
     6961 gatacctata aaaccgaaac agctagagca gctttagagg cgggtgccga tattctcaat
     7021 gatgtttggg caggtttgta cgacggtcaa atgtttgcct tagcagccga gtacgatgcg
     7081 cctatcatct tgatgcataa ccaagacgaa gaagtttatc aagaggtaac acaagacgtt
     7141 tgtgattttc taggcaatag agcacaagca gctcttgatg ctggcgtgcc aaaaaacaat
     7201 atttgggttg atccaggatt tggatttgcc aaatctgttc aacagaatac ggagttatta
     7261 aaaggattgg accgcgtctg tcatttgggc tatcctgtct tgtttggtat ttcgagaaag
     7321 cgtgtcgtag atgccttgtt aggcggaaac accaaagcta aagagcgaga cggagcgaca
     7381 gcagccttgt ctgcttatgc ccttggaaaa ggctgtcaga ttgtacgcgt acacgatgtc
     7441 aaggctaatc aagacattgt ggctgtgttg agccagttga tgtgaggact tatggataaa
     7501 atcgtattag aaggttgccg tttttatggc taccatggag cctttaaaga agaacagacc
     7561 cttgggcaaa tttttctggt tgatttagaa ttgtcagtcg atttgcaagc agcttctttg
     7621 tcagaccaat taacagatac ggtccattac gggatggtgt ttgatagtgt ccgtcagtta
     7681 gtggaagggg aaaagtttat tttgattgaa cgtttagcag gtgcgatttg tgaacagctt
     7741 tttaacgaat ttccgcctat tgaagccatc aaagtggcca ttaaaaagga aaacccaccg
     7801 attgcaggcc actataaggc agttggtatt gaattggaga gacagagatg accattgttt
     7861 atttaagttt aggcaccaat atgggggacc gagcagctta cttgcaaaaa gctcttgagg
     7921 ctttagctga cttacctcaa acacggttgc ttgctcaatc atccatttat gaaacgactg
     7981 cttggggcaa gacaggccag gctgattttc tcaatatggc ctgtcaatta gatacacaat
     8041 taaccgcggc tgatttctta aaagaaacac aagctattga gcaatctctt ggtcgtgtga
     8101 ggcatgaaaa atggggatca agaactattg acattgatat cttgctattt ggagaagagt
     8161 tttatgacac aaaggaattg aaagtgccac acccttatat gactgagcgt gcttttgttt
     8221 taatcccctt attggaattg cagccagatt taaaattgcc tcctaatcat aagtttttaa
     8281 gggattatct tgcagcttta gatcaatcag atatcacgct cttttcagct caacagacag
     8341 agttctagac tctgttttcg gagtaatgcc tgctataagc caatccataa agttttgcca
     8401 aattcatcgt tattttttct aaaatttgat ataatagtat cggctttatg ccgatttttt
     8461 tacgcgtata agaagtgata aaagaaagaa gataactatg ataactgaac ttcatgggat
     8521 tgatattcga gagaatgaac ccctaaaaca ttacacttac actaaggtag gtggcccagc
     8581 agatttcttg gcttttccta gaaatcacta cgagctatca cgcatcgttg cttatgccaa
     8641 caaagaaaat atgccttggc ttgttttggg aaatgccagc aatctcattg tgcgagatgg
     8701 tggtattcgt ggctttgtca tcatgtttga taagctaaat gcagtgcact taaatggtta
     8761 taccttagaa gccgaagctg gtgccaatct gattgaaaca acgaagattg ccaaattcca
     8821 tagtttaaca gggtttgaat ttgcatgtgg cattcctgga agcattgggg gtgctgtttt
     8881 tatgaatgcg ggtgcctatg gtggggaaat atcacatatt ttcttatccg ctaaggtgct
     8941 tacctcaagt ggagagatca aaaccatttc agctagggat atggcttttg gttaccgtca
     9001 ctctgccatt caagaaacag gtgacattgt catttctgct aagtttgctc ttaaaccggg
     9061 taattatgat acgatcagcc aagaaatgaa tcggttaaat caccttcgcc aactaaaaca
     9121 acctttagaa tttccatctt gtggatctgt gtttaagcgc ccgccaggac actttgcagg
     9181 tcaattaatc atggaagcaa atcttaaagg gcatcggatt ggtggtgtgg aagtttccga
     9241 aaaacacgct ggttttatga ttaatgtggt agatggcaca gctaaagatt atgaagattt
     9301 gattgcttat gtcattgaga cagttgaaaa ccattctggt gtcaggcttg aaccagaagt
     9361 tcggattatt ggggaaaacc tgtaaccatt attgataatt aaagaaagta ccgtggagga
     9421 tttatgacaa ttgactaagc caattatcac atttaacaat gtttcaaaaa catttgagga
     9481 cagtggaaca caggttctaa agaacattaa ctttgacctt gaagaaggaa aattttacac
     9541 cctgcttgga gcttctggct ctggaaaatc aactatttta aatattatgg cgggcctact
     9601 agatgccagc agcggagaca tttatttaga tggggaacgt attaatgatt tgccgattaa
     9661 taaacgtgac attcataccg ttttccaaaa ttatgccctc ttccctcata tgactgtttt
     9721 tgagaatgtt gcctttgctt tgaagctgaa gaaagtggac aagaaagaga ttgctaagcg
     9781 cgtgaaagaa accttgaaaa tggttcaatt agaagggtat gaaaatcgtt ctatccagaa
     9841 gttatctggt ggtcaacgtc aacgtgttgc cattgcgcgt gcgattatca atcaaccgcg
     9901 tgtggtctta cttgacgaac cactctcagc ccttgatttg aaattaagaa cagagatgca
     9961 atatgaatta cgtgaattgc aacaacgtct aggcattacc tttgtttttg ttactcacga
    10021 tcaagaagaa gccttggcca tgagtgattg ggtttttgtc atgaatgaag gtgaaattgt
    10081 tcagtcggga acaccagtgg atatttatga tgagccaatt aatcattttg ttgctaattt
    10141 tattggagaa tctaatatta ttaacggtac catgattgaa gactatcttg tctcctttaa
    10201 cgggaaagaa tttgaatctg tggacggtgg gatgcgccct aatgagcctg ttgaagtggt
    10261 tattcgtcct gaagatcttc aaattacttt gccagaagaa gggaaattac aagttaaggt
    10321 tgatacccaa ttattccgcg gggttcacta cgaaattatt gcctatgatg aattgggtaa
    10381 tgaatggatg attcattcta cccgcaaagc tatcgaggga gaagttatcg gattagactt
    10441 tacccctgaa gatcttcata tcatgcgtct taatgagact gaagaggaat ttgatgcccg
    10501 tattgaagaa tatgtggaaa tggatgagcc tgaagatgga ttgattaatg ccattgagga
    10561 ggagcgtaat gaagaaaacc tctagtcttt tttcgattcc ttacttctta tggattctct
    10621 tctttgttgt ggcaccagtc actctcttgt tttacaagtc cttttttgac atagaagggc
    10681 gcgtgacctt agccaattat gaaacctttt ttagctcttg gacctatttg agaatgagtg
    10741 tgaattctat tttatacgct ggtattatca cactcgtcac gctcttgatt tcatatccta
    10801 cggctctctt tttaacgcgc ctaaagcaca agcagttgtg gcttatgctc attattttgc
    10861 caacttgggt aaacttattg ctaaaagcct atgcctttat gggaatcttt ggtcaacaag
    10921 gaggaattaa cagcttttta acctttatgg ggattggccc gcagcaaatc cttttcacgg
    10981 atttctcctt catttttgta gcctcttaca ttgagctccc ttttatgatg ttaccgattt
    11041 ttaacgcttt ggatgatatt gaccataatg tcattaatgc cagtcgcgac ctaggagcta
    11101 gtgaatttca ggccttctca aaagttattt ttcccctttc tttaaatggg gttagggcag
    11161 gtgttcagtc tgtctttatc ccaagtttga gtctctttat gttaacccgt ttgattggtg
    11221 gaaaccgcgt gattacactt ggtacagcca ttgaacaaca ttttttgacc acccaaaact
    11281 ggggaatggg atcaaccata ggtgtggtgt taatcttaac catggttgct attatgtggc
    11341 tcacaaagga gaaaagtaaa tgaaaaaatt tgccaatctt tatttagcga gtgtctttgt
    11401 tttactctac attcctattt tttatttgat tttctattct ttcaacaaag gtggggatat
    11461 gaatggtttt acaggattta cccttgagca ttaccaaacc atgtttgagg atagtcgtct
    11521 catgacaatc ttactgcaaa cctttgttct tgcttttagt agcgctctac tagcaacgat
    11581 tattgggacc tttggagcta tctttatcca ccatgttaga ggtaagtacc aaaatgccat
    11641 gctatcagcc aataatgtct tgatggtatc accagatgtc atgattgggg cttccttttt
    11701 aattcttttt acatcattga agtttcagct gggcatgtct tcagttttat taagtcatat
    11761 tgctttttcg attcctattg tggttttgat ggtattgccg cgcttgaaag agatgaatca
    11821 agacatggtc aacgccgctt atgatttggg agctaattat ttccaaatgc tcaaagaagt
    11881 catgctgcca tacttaacac cagggattat tgcaggttat tttatggcct ttacctattc
    11941 cttagatgat tttgcagtga ctttcttttt gactggaaat ggttttacta ctttatctgt
    12001 tgagatttat tcgcgggctc gtcagggaat ttccttggat atcaatgctt tgtcaaccat
    12061 cgttttcttt ttctccatcc tcttagtgat cggttattat tatatgtcac aggacaagga
    12121 ggaaaaacat gcgtaaactt tattcctttc tagcaggagt tttgggtgtt attgttattt
    12181 taacaagcct ttctttcatc ttgcagaaaa aatcgggttc tggtagtcaa tcggataaat
    12241 tagttattta taactgggga gattacattg atccagcttt gctcaaaaaa ttcaccaaag
    12301 aaacgggcat tgaagtgcag tatgaaactt tcgattctaa tgaagccatg tacactaaaa
    12361 tcaagcaggg cggaaccact tacgacattg ctgttcctag tgattacacc attgataaaa
    12421 tgatcaaaga aaacctactc aataagcttg ataagtcaaa attagttggc atggataata
    12481 tcggtaaaga atttttaggg aaaagctttg acccacaaaa cgactattct ttgccttatt
    12541 tctggggaac cgttgggatt gtttataatg atcaattagt tgataaggca cctatgcact
    12601 gggaagatct gtggcgtcca gaatataaaa atagtattat gctgattgat ggagcgcgtg
    12661 aaatgctagg ggttggttta acaacttttg gttatagtgt gaattctaaa aatctagagc
    12721 agttgcaggc agccgagaga aaactgcagc agttgacgcc gaatgttaaa gctattgtag
    12781 cagatgagat gaaaggctac atgattcaag gtgacgctgc tattggaatt accttttctg
    12841 gtgaagccag tgagatgtta gatagtaacg aacaccttca ctacatcgtg ccttcagaag
    12901 ggtctaacct ttggtttgat aatttggtac taccaaaaac catgaaacac gaaaaagaag
    12961 cgtatgcttt tttgaacttt atcaatcgtc ctgaaaatgc tgcgcaaaat gctgcatata
    13021 ttggttatgc gacaccaaat aaaaaagcta aggccttact tccagatgag ataaaaaatg
    13081 atcctgcttt ttatccaaca gatgacatta tcaaaaaatt ggaagtttat gacaatttag
    13141 ggtcaagatg gttggggatt tataatgatt tatacctcca atttaaaatg tatcgcaaat
    13201 aagcactcaa aaaggctggt atttctccag cctttttaat gtatcgacag atgacgtcac
    13261 cagtacacgc gatagggacg accaaccttg gtaaaaattt gttggctgtt cagttgctta
    13321 ttttcttcta aataagcaat gtatttacgg acagagacat gggataagtg acaagcggaa
    13381 gctaattctt ggatagtaaa aggttgatca aaggctttga tattttctat aatccactgg
    13441 aaagtggatt cagataagcc tttttctaac aattgtttgt ttttagtgtc ttttttgcta
    13501 gtcaaacatt tcagttggtc aatttgggcc tgctctagtt gctgattggc caaatgttct
    13561 cgatgcgtaa caaactgttg gatgctttct tgaaagcgtt caaaggtaaa gggcttgatg
    13621 aggtagtcga taattcctaa atgaaaacca tctcggataa tattcccatc atttgcggct
    13681 gaaataataa tgacttcgca aggaatatgc tgagcacgcc atttctccaa aaattgaatc
    13741 ccattgccat ctgtaatatg aatatccagt aaaatgaggt caattgcgta atcagtaagt
    13801 atgctttgaa cagctttcat agagtcgctg gatataatgc gatcaaaaag atttagtttt
    13861 tcgaggtaat tgcgatggat aaaatccacc ataggatcat cttcaatgat taaaacgttc
    13921 atgttgccat cctacataag gtgttgtcac tgagaactgt aactcggtta cggtttgatg
    13981 ttgatggaat tgggaatttg tatcggtcaa gagctgttga aaataaggta agtcaaaaat
    14041 aggctgtacc cgctccaaat caatccaatt gtctgtcaag gtgtaatggc tgattaaatg
    14101 attctgatca tgttgaatac tcaaaacgag tgatttgggt ctcagtgtgg ttagaagatt
    14161 agtatggata aagcggtaaa tcatcaaacc attattaagt tgattcttgg taacagaatg
    14221 aggaatctcg cttaaaacat caatcttgag atggacattt aattcttggt atttctcttg
    14281 ctctccaatg agaaaactag ctagaagagg ctccttaaca aggacagata agctagtgag
    14341 aatttcattt tcgggctcca aaatactgtc taaataaatt ttcaattggt cataataagc
    14401 aatatctact aagccataaa taacatgcaa ttgattcatg aatttatgag tttgggcttg
    14461 tagagcagaa gcataggcgg tggtataggc tagctgatcc aaggtatcaa tagccttaac
    14521 tgcttctcgc atgaaaataa tgtggcctcg gtgatcgttt ttgacacaga tgggtgaaat
    14581 ggctaacaag tagtcttcct caccataccg aaagagttgt tcatgcccct cttgcaaact
    14641 aagttttgga aaatcaggaa agaggtgatt gaaactgttg ccagtaggtt ttcccaactg
    14701 gcattttcga gcgattaact cttgtccagc ctgattgcat aattgtaaaa taccagtttt
    14761 atctaccaca aatacggcgg cttcgatttg atccaacatg gcattacgct cttcaaagag
    14821 ctggtaaatt tcacttggtt ctaactggtg cagttgacgc ttgagtcgaa aagaaataaa
    14881 actggtgacc agtaaactaa taagaagaca aaggaataaa gacagggtat aattgcgttt
    14941 tgacgttaaa gccacatcat ttagggtagt tagtttaatg ccaaccgcaa tagcaccaat
    15001 ctgtttgtca ccgtcaaaca caggtactag atagcgaagt gactttccta aagtgccctt
    15061 ggcggtagag ataacctttt ttcctgctag aacagcctcc tcatcacctc cttgaaaagg
    15121 ttttccgata tttttcgggt taggacgggt gagtcgaatc cctttcatgt tcatcacgac
    15181 cacataatca aggttataga tggaggcaat ggagttggta taggctgttg ttttagcatt
    15241 tggttggttg tttaggagta attctttaat tgcttgatga cttgctaaca tttttccagt
    15301 tgacgttagc aaatgggttt cctgattttt gatggattgg tgcgtatcat gtagcataat
    15361 cccgtaaaat agggaggttg tgacaacaat catagatacc aaaatcaacg ataaactggc
    15421 ccataaacgt agtggttttt tcataaggtc tcctctttat tgagtataac acgataagaa
    15481 atgacctgct agcttaaatt aattaaataa cttgaaaaaa ttaaagtagt taaacaactt
    15541 aaataaacta tgcttttgac atgttctctt ttatactagt cttatcacta aaggaggttg
    15601 tttcatggaa acaataagca agaaaatgcc tcaaaaagac ttgtctgagc attcaaaagc
    15661 atggcaaaat aggcgaatag gctccgttcc cttgcctgtt tatctggttt tagctacctt
    15721 aattttgatc acaggctggc tccaacaatt gcctgttaat atgctgggtg gctttgctgt
    15781 tattttaacc cttggttggc tactaggaac cattggagcg accattccag gcctcaaaca
    15841 ttttggtggc ccagctattt tatcactctt agtcccttcg attttggttt tttttaatct
    15901 cctcaacccc aatgtcttag aagcgacgaa tgtcttgatg aagcaagcca attttcttta
    15961 tttttatatt gcctgtctgg tctgcggtag tattttaggc atgaatcgaa aaatcttgat
    16021 tcaaggtctt tttcggatga ttattcccat gttacttgga atggtttgtg caatgggtgt
    16081 agggactttg gttggcgtca ttttaggatt agactggcaa cataccctct tttatgttgt
    16141 gacgccagtc cttgctggag gaattggtga aggtatttta cccttatctc ttggttacag
    16201 tgccattaca ggagtcggaa gtgagcaatt agtggctcaa ctgattccag ccaccattat
    16261 tgggaatttc tttgctatct tgtgcacggc tttgctcaat cgttttggag agaagcaccc
    16321 aagttattct ggtcaagggc aactggttaa aattggtcac agcgaagaca tgtctgatgc
    16381 cctaaaagac aattctggag ccctagatgt taaattaatg ggggcaggcg ttttaacagc
    16441 ttgttcactt tttattgctg gtggcttgtt acagcattta acaggttttc caggaccggt
    16501 gttgatgatt attctggcag ccttcttgaa atacctgaat gttattcccc aagaaaccca
    16561 aaatggtgcc aagcaacttt ataaatttat ttccagcaac tttacttttc cgttaatggc
    16621 agggcttgga ctgctttata tccccttaaa agaggttgtg gcaaccctta gctggcaata
    16681 ttttattgtg gtgattagtg tggtgttaac ggttgtcagt gtcggtttct ttgtctctcg
    16741 cttccttaac atgaaccctg ttgaagcagc tattatttca gcttgtcaaa gtggtatggg
    16801 tggaacaggg gacgtggcta ttttgagtac ggctgaccgc atgaatctaa tgccgtttgc
    16861 ccaagtcgct actcgtctag gtggggctat taccgttatc accatgacag ctattttaag
    16921 aattattttt agttaatcca aaccaataaa aaagagaggt atttcctatg aaaaatcaat
    16981 taggtcaact tgcccttgaa caagctaaaa cgttcggagg taaattggaa gttcaaccaa
    17041 aagttgacat tacaaataaa catgatttga gcattgccta tacaccaggt gtagctggtg
    17101 tgtcatctgc catcgcaaaa gataaaacat tagcatatga cttaaccacc aagaaaaata
    17161 cagtagccgt tatcagtgat ggtactgcgg ttcttggtct gggggatatt ggccctgaag
    17221 cagccatgcc agttatggaa ggaaaagcag ccctttttaa agcatttgca ggtgtcgatg
    17281 ctattccgat tgtattagat accaaagata ctgaagaaat tattagtatt gtcaaggcgc
    17341 ttgcaccaac ctttggtggt attaatttag aggatattag tgcgccccgc tgttttgaaa
    17401 ttgagcaacg cttaattaag gaatgtcata ttcctgtttt ccatgacgat cagcatggaa
    17461 cagctattgt ggtgttagca gccatcttta atagtttgaa acttcttcaa aaatcccttg
    17521 atgaggtatc catcgtggtg aacggtggtg gatctgcggg attatccatt acccgtaaat
    17581 tattagcagc aggtgctacg aaagtgacag tagtggataa atttggcatt atcaatgaac
    17641 aagaggcaga tcagttagca ccacaccatt tggatatagc gaaagtcact aatcgtgagt
    17701 ttaaatcagg taccttagaa gatgctcttg aaggagcaga tatcttcatt ggcgtatcag
    17761 caccaggagt cttaaaagca gagtggatta gtaaaatggc agcgcgccca gtaatttttg
    17821 ccatggccaa ccctattcca gagatttacc ctgatgaagc ccttgaagcg ggggcttaca
    17881 ttgtaggtac tggccgtagt gattttccta accagattaa taatgtcctt gcctttccag
    17941 gtatttttag gggagcatta gatgcacggg caaaaaccat tactgttgag atgcaaattg
    18001 ctgccgcaaa aggtattgcc agtcttgttc caggtgacgc tttgagcact actaatatta
    18061 ttccagatgc ctttaaagaa ggagttgctg agattgtcgc taaaagcgtc aggtcggtag
    18121 tccttaaatc atagaatata tgaaaaaaag agaaagttat actttaaatc acaaaataag
    18181 tagaagcata tttctagctt ctacttattt tgtgattagc taatatcatt atcaataata
    18241 actttcaaga catttagtag catttcagtt gtattggcat taacaagtcc agtgtttaag
    18301 gtgatatttt taatccataa ttcatccagg ttgaaatcaa ctggtttccc atgaacccca
    18361 acgttagcaa tatgcccacc tactgatata attttttggc aaatatcaaa tgtcgcggga
    18421 taaccaacac actccatgga aatatcgaca ccacgtccat tggttatatc gtcaatgata
    18481 gccttgactt cttcactact tcctgaacaa atcgtgtgtg tggctccaaa cgttttagct
    18541 gcttcaaggc gattttgaga taagtcgacc ataataatat tggctggaga gaaaagttga
    18601 acggttagta gggctgcgag tcctactgga ccggcaccaa caatacaaac attatctcca
    18661 ggcttgacat gtgatggcaa gacaccaatt tcatatgaag ttggtaaaat atcagaaagc
    18721 atgaccagtg cctcatcatc aatcgtatca ggagcatgat agagactacc atctgcatgt
    18781 gggatatgga catattctgc ttgtgttcca ttaattaaat ggccaagaat ccaaccgcca
    18841 tcttcacaat gtgacgaaag gcctcgttta caataataac aggtatgaca tgatgtgacg
    18901 caagaaataa tcactttatc tccgatttta aagttagtga ccgcatcacc gatctcctta
    18961 acaattccaa ttccttcagg accaaggatt gttccttctt tacatgcggg aacatcccca
    19021 cctaagatat gcaaatcagt tccacaaata gagtggtttt taccaactgg acgatatgta
    19081 ggctttataa tgactggttt tggtttgtct atgagctgta gatttccggt tgaaaggtaa
    19141 gttgctgctt tcatatttaa ctctttataa ggttgagagg taatcaacca attcattttt
    19201 acacagatat tataatagtt taagaaaggg ttttcaatat tttttaaatc gattatgata
    19261 attttttaga tcatctttgt tgaataactt catccttatt cagaacttaa agggcactat
    19321 tataagaatt tgaaaccgct ttacttaaac cgtgtatttt tgtacaataa tgattaagga
    19381 ggtttttaga tgaaaaaaga atttaccagt atcctgttta cagttagttt ttgtggaatc
    19441 atagcattgc ctgtcgaagc tagtggccct aaagttcctt atacacaaga aggtataacg
    19501 gccatttcaa atcaggcaac agtaaaattg atttctattg ctgatatcgc gagcagttta
    19561 gaaggacaaa aacctataac ggttagtttt gatatcgatg atactttgtt atttactagc
    19621 caatattttc agtacggcaa agaatatata actccaggct catttgattt tttgcataag
    19681 caaaaatttt gggacttagt cgctaaacgg ggagatcagg attctattcc aaaagagtat
    19741 gcaaagcaac taatcgcaat gcatcaaaaa cgaggagata aaattgtctt tattactggc
    19801 cgaactcgag gatcgatgta taaaaaaggt gagattgata aaacagctaa gtcactggct
    19861 aaagatttta agttagacaa gccaatagct atcaattata caggagataa agctgtgaag
    19921 ccgtatcagt atgataaaac atattatata aagaaaaacg gctcacaaat tcattacgga
    19981 gatagtgatg aagatataaa tgctgccaaa gaggcaggtg caagacctat tcgcatttta
    20041 agagctccta attcaaccaa tcttcctttg ccaaaagctg gaggatacgg agaagaggtt
    20101 ttagaaaatt cggcttatta gtcaaatatt tgtgtaagaa aggaagtaac agtaatttct
    20161 ttcatcaccc cactgagtaa ccaatagccc agtgggtttt ttgggttaca gaaatcagtt
    20221 ctaaaaaaga atcactggta acttaaggtt gtctttgata caatggtaac agtgtattta
    20281 aaagaaagag aggataacat ggaaaaccat aaaaatgaat tcaccttttc caacaaatca
    20341 attattgcct atgtttggcg tggtgttatg gttggtatta ttgctggtgt tattgttagt
    20401 ttattccggt tgttaattga agtgacagct gactgggtga ttgaatggta ccgatacgct
    20461 catatcaatt cgctgttgct acttcctata ctaagtgtca gtttgttagc tgttcttttt
    20521 gtaggttttt tagtaaagtc tgattctgat ataaaaggat cagggatccc ccatgtcgaa
    20581 ggagaactaa aggggctaat gtccccagat tggtggagtg tcctttggaa aaaattttta
    20641 ggaggaatca tggctatttc tatgggattt atgctcggtc gtgagggtcc ttcgattcag
    20701 ttaggtgcta tgtcagccaa aggattagct aagtttttaa aatcgagtcg tttggaaaag
    20761 cgagtgctaa ttgccagcgg agcagcagca gggctatcag cagcttttaa tgctccaata
    20821 gcaggcctct tgtttgttgt tgaagaaatt taccatcatt tttcgcgttt aatttggatt
    20881 acagcgctag tggctagctt agtggctaat tttatttctc tgaatatttt tggattgaaa
    20941 ccagtcttag caatgtcaga ggcgatgcct tttttagggc taaatcaata ttggttgttg
    21001 cttttacttg gattatttct aggctgctta ggctatttat atgagatagt tatccttaat
    21061 tttaataaac tttatgttat tttagggaat tggttacatc tgccagctta tttttacggt
    21121 attattatgg tttttctaat tttaccgatt ggctattatt taccacagtt acttggagga
    21181 gggcatggtt taatactttc tttatccaat caacaattgc ctttgatgac tatcttcttt
    21241 tattttatta ttcgatttat tgttagtatg tttagttatg gaagtggctt acctggaggt
    21301 atttttttac ccattttaac tttaggtgct ttggcaggcc tattatttgg gcaaatagct
    21361 tctcaattag gtttattgaa ccaatctttt ctttctcttt ttttgatatt agggatggca
    21421 gggtattttg cggcaatttc aaaagcacca ctgacaggca tgattttggt aactgaaatg
    21481 gtaggtgatt tgaaaccctt aatggccata gctgtagtta cttttgtctc ctatttagtg
    21541 atggatttgt taaatggaca gccaatttac gaagctatgc tagataagat ggcaatgaag
    21601 catccaacta atcttgttga accaacatta attgaattga ctgttggtga taagattgca
    21661 gggaaatatg ttaaagagtt aaagcttcca gaaaatgtct taattacaac ccaaattcac
    21721 catcaaaaat ctcaagttgt gtcaggtaac acgcgccttt tgtcaggagc aactatcttt
    21781 ttggtcgtta atgaagctga tacaggtttt gtccgtgagg tattgatgta ggtattaatt
    21841 tatcacaatt cggaaagttc gagcttgtct tttagactaa gtcttcgaac gttttactgc
    21901 tagagtttca tctgaaccaa agtgaaacgc ttttgatgaa gtcaagtagg ctttttgtta
    21961 tactaacaat aaagataagc taaggaggct gagatgttag aaattgtttc agaagagcta
    22021 cgtcactatc aagaacaaaa attgattgag taccgtaaca aaaatcagtt agccccaaaa
    22081 ggaggcattg tatttgcagg tgattctctc attgaatttt ttcctttaaa aaaagctttt
    22141 ggttcatgtt tgcctattat taatcgaggt attgctggga tagatagtca gtggttattg
    22201 cgccattttt ctgtccaaat tactgattta gagcctaaac atattttttt attaattggt
    22261 tgtaatgata tcgggtttgg atatgataaa tgtcatattg tcaagactat tgtagagctt
    22321 attagccaga ttcgtagtca ttgtgtctac agtcagattt atctcttatc tttattacca
    22381 gtttccaaca atccccgata tcagaagact gtcaaaataa gaacaaatgc tatgattgat
    22441 gctattaata aagatttagc aatgattcca actatagaat ttattaatct taatacttgt
    22501 cttaaagacg aaaaaggtgg tttatcagat gagaatactc tagatggttt acatcttaat
    22561 tttccagctt atgctaaact agctgaaatt attcaagctt atatctagca aataaatgta
    22621 ctaatagcga tgaggcaaga agtcagatag cagatggttc atcaatcaat taagaaaaaa
    22681 gttatcaaga taccattaaa aatagttgct caaagttctt tcaaatcaaa agattatgat
    22741 atcgctatgt tgagttttag tgtataatga taattaaagg agttaccatg aaatttgaaa
    22801 aaaaacaagt cttctattta gtccttacgt ttatcttatg ctatgggatc ctagcaaact
    22861 ggcgaaatgg aacggctatc gtcacgacta tttacaaaac aagccttcct tttttttatg
    22921 gagcagcagg tgcctatatt gttaatattg taatgagcgc ttacgaaaag gtatatgttt
    22981 atatctttaa agactggtca catgtcttaa aagtaaaacg gggtatttgt ttactgttag
    23041 cgtatttgac attttttatt ttaattactt ggattatttc cattgtcatt ccagacttaa
    23101 ttacaagtat tagtactcta acaaaatttg atactataac gatacaagaa gttgttaaca
    23161 atcttgagca caataaactg ttagcacgta ctattcagta tattggtggc gatggcaaac
    23221 ttacagagac gattgctaac tatagtcaac agttattaaa gcaatttctt actgttttga
    23281 caaatatttt aacatctgtc acggttattg cttctgcaat tattaatctt tttattagtt
    23341 ttgttttttc gttatacgtt ttagcaagta aagaagatct ttgtcgtcag ggaaatactt
    23401 tagtagatac ttatactggt aagtacgcta aacgcattca ttacttgtta gagttgttgc
    23461 atcagcgttt tcacggattt tttgttagtc agaccttaga agctatgatt ttaggttcgc
    23521 tgactgctag tggcatgttt atattaagat taccatttgc agggactatt ggcgttttag
    23581 tagcttttac agcattgatt ccagttatag gtgcttcaat tggagcagct attggattta
    23641 ttttaattat gactcagtcc atgtcacagg ccattatttt tatcattttt ttaattattt
    23701 tgcagcagat tgaaggtaat tttatttatc cgaaagtagt tggtggatcg attggattac
    23761 cggctatgtg ggtattaatg gcaattacaa taggcgcttc tttaaaggga atagttggta
    23821 tgattattgc agttccttta gcagcgacac tttatcaagt gattaaagat aatattcaaa
    23881 aaagacaagc tattcaaaaa aaacaagttt cctaaaagga acttgttttt ttgtcgaata
    23941 aaaagatgga ggtaatatga taaatgtatt ctataaaatg tgatgataat aaagccatgc
    24001 caagagaacg tttgatgcga ctaggagcag agtctctaag taatcaagaa ttattagcaa
    24061 ttttattacg aacaggtaat aaagaaaagc atgtcttaga gctgtcatcc tatcttttat
    24121 cgcatttaga cagtctggca gattttaaaa agatgtcttt gcaagaatta caacatttgg
    24181 caggtatagg aaaagttaaa gcgattgaaa ttaaagctat gattgagttg gtttcccgaa
    24241 ttttagcgac cgataagaca ttaactgata gcgtattaac cagtgttcag gtcgctgaaa
    24301 aaatgatggc agctttagga gataaaaaac aagagcattt agtcgtattg tatttagata
    24361 atcaaaatcg tatttttgaa gaaaaaacta tttttattgg gactgtccga cgttcacttg
    24421 cagaaccaag agaaatttta tactatgcct gtaaaaatat ggcgactagt ctcattgtta
    24481 ttcataatca tccttcagga aatattgaac ctagttctaa cgattattgc tttactgaaa
    24541 aaataaaacg atcatgtgaa gatttaggca ttatctgtct agatcacatt atcgttagct
    24601 ataaagatta ttatagtttt cgagaaaaat caaccctttt ttaaatcaga agtcattgac
    24661 gaataagcgg aacaattgta gatcttcatc tcgaacacct tggagaagtt caggatgcca
    24721 ctgaacacct aaaaaaggaa tggcatcgtt ggttgatatg actgcttcga tggtaccatc
    24781 acgtggatct ctagcgatta cttttaaatc cttagcaata gtttttaaac tttggcgatg
    24841 aaaagaatta attaatgtct tatgaccgta aataggatat aagatagagt ctggctcaat
    24901 aatcatttcg tgtgataaga aatcagaagg cgcttcttgc caatgtgagt caatgtgttg
    24961 gttaagattt cctcctaagg caacattcat taactgtgta ccacgacaaa tccctaagat
    25021 tggttttttt aatgttatag cctccttgat gatagctagc tcaaatgtgt ctcgctctgg
    25081 tgaaaaatca tcatcaaaag cagccttctc ttcttgataa tattttgggt caacgttttg
    25141 cccaccaatt aaaatgattt tatcaaccat agatacataa gttttagcgg cggcttcatc
    25201 accaatgggt aaaagaagcg gcagcccccc agattgagtg actgcctgta caaatccagt
    25261 aggtgcatat gaccatggga gattatccaa agccatattt agtctttgat ttgcagttat
    25321 tccaataatc ggtttggtca ttatacctcc ttaatctaga tgattttgat tcataaaata
    25381 gagtagtgtc tggagctcac tggtcaaatc gacagattga acaataactc ctttaggaac
    25441 ttgtaggtga acaggagcaa aactcaagat tcctttgatt cctgcttcaa taagttgatc
    25501 agtaacttct tgagcatgaa tgctgggaac cgttaagata gccgtttcaa tgtcagtgtt
    25561 tgcaatccgt tctttgacgg atgagatacc atggactgga atattatcag ctgttttagt
    25621 accaacaaga gcgttatcat cggtatcaaa acccatggca atttgcatct tattacgatc
    25681 atggaagcgg taatgtaata gagcacgccc tatattaccg catccaacta agataacgtt
    25741 tgttgtagag tgatcattaa gcaagtcagc aaaaaaattc atcaatttgg taacatcata
    25801 gccaaaccca cgacgcccca attctccaaa ataagaaaag tcacggcgta cagtagcaga
    25861 gtcaattccc attgcatctg caatttgttt agaactggct ttttcaactt ggtcagcatg
    25921 gaatcgttta aaaatacggt aataaagtga taagcgcttt gcagtcgctt ttggaataga
    25981 tttgtcaata accactattt tactccttgt agatttctcg ttaaatatgt ctgtctttat
    26041 tgtaacaaaa agattgtgaa aaatgcaaca ttttgattgt ttttgtatga gttaaaaaag
    26101 ctaaaatt
//
LOCUS       DATXQK010000021        22404 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.021, whole genome shotgun sequence.
ACCESSION   DATXQK010000021 DATXQK010000000
VERSION     DATXQK010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22404
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.021"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            19..1215
                     /locus_tag="VC394_000972"
     CDS             19..1215
                     /locus_tag="VC394_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002996017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEP5994449.1"
                     /translation="MKKRILSAVLVSGVTLGAATTVGAEDLSTKIAKQDSIISNLTTE
                     QKAAQNQVSALQAQVSSLQSEQDKLTARNTELEALSKRFEQEIKALTSQIVARNEKLK
                     NQARSAYKNNETSGYINALLNSKSISDVVNRLVAINRAVSANAKLLEQQKADKVSLEE
                     KQAANQTAINTIAANMAMAEENQNTLRTQQANLVAATANLALQLASATEDKANLVAQK
                     EAAEKAAAEALAQEQAAKVKAQEQAAQQAASVEAAKSAITPAPQATPAAQSSNAIEPA
                     ALTAPAAPSAGPQTSYDSSNTYPVGQCTWGAKSLAPWAGNNWGNGGQWAYSAQAAGYR
                     TGSTPMVGAIAVWNDGGYGHVAVVVEVQSASSIRVMESNYSGRQYIADHRGWFNPTGV
                     TFIYPH"
     gene            1468..2430
                     /locus_tag="VC394_000973"
     CDS             1468..2430
                     /locus_tag="VC394_000973"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEP5994450.1"
                     /translation="MSYSDLKLFALSSNKELAEKVASAMDLQLGKSTVRQFSDGEIQV
                     NIEESIRGHHVFILQSTSSPVNDNLMEILIMVDALKRASAEKISVVMPYYGYARQDRK
                     ARSREPITSKLVANMLEVAGVDRLLTVDLHAAQIQGFFDIPVDHLMGAPLIADYFDRH
                     GLVGEDVVVVSPDHGGVTRARKLAQFLQTPIAIIDKRRSVDKMNTSEVMNIIGNVSGK
                     KCILIDDMIDTAGTICHAADALAEAGATAVYASCTHPVLSGPALDNIQRSAIEKLIVL
                     DTIYLPKERLIDKIEQISIADLVAEAIIRIHEKRPLSPLFEMGN"
     gene            2616..3371
                     /gene="recO"
                     /locus_tag="VC394_000974"
     CDS             2616..3371
                     /gene="recO"
                     /locus_tag="VC394_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEP5994451.1"
                     /translation="MQLTESLGIVLFNRNYREDDKLVKIFTEVAGKQMFFVKHISRSK
                     MSSIIQPLTIADFIFKLNDTGLSYVVDYSNVNTYRYINNDIFRLAYASYVLALADAAI
                     ADNESDSHLFTFLKKTLDLMEEGLDYEILTNIFEIQILDRFGISLNFHECAICHRTDL
                     PLDFSHRFSAVLCSEHYYKDNRRNHLDPNVIYLLSRFQKITFDDLRTISLNKDIKKKL
                     RQFIDELYHDYVGIKLKSKTFIDNLVKWGDIMK"
     gene            3474..4481
                     /gene="plsX"
                     /locus_tag="VC394_000975"
     CDS             3474..4481
                     /gene="plsX"
                     /locus_tag="VC394_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEP5994452.1"
                     /translation="MKRIAIDAMGGDNAPKAIVEGVNQAIEAFSDIEIQLYGDQTKIN
                     SYLIQSDRVAIIHTDEKIMSDDEPAKAVRRKKKASMVLAAKAVKEGKADAIISAGNTG
                     ALLAVGLFVVGRIKGVDRPGLLSTIPTVTGLGFDMLDLGANAENTAKHLHQYAILGSF
                     YAKNVRGIANPRVGLLNNGTEETKGDPLRKATYELLTADNTISFVGNVEARELMSGVA
                     DVIVSDGFTGNAVLKSIEGTAISIMGQLKQIINSGGIKTKIGASLLKSSLYEMRKTLD
                     YSSAGGAVLFGLKAPVVKSHGSSDVKAIFSTIKQVRTMLDTNVVGQLVEEFAKETQVN
                     D"
     gene            4474..4716
                     /locus_tag="VC394_000976"
     CDS             4474..4716
                     /locus_tag="VC394_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017228.1"
                     /note="carries the fatty acid chain in fatty acid
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEP5994453.1"
                     /translation="MTRQEIFERLINLIQKQRSYLSVAITEQTHLKNDLAVDSIELVE
                     FIINVEDEFHLAIPDEDVEDMVFMGDVLDYLVQRLT"
     gene            4867..5571
                     /locus_tag="VC394_000977"
     CDS             4867..5571
                     /locus_tag="VC394_000977"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEP5994454.1"
                     /translation="MTNQLIYKGKAKDIYSTKDENVIRTVYKDQATMLNGARKETIDG
                     KGALNNQISSLIFEKLNKAGVVTHYIEQISKNEQLNKKVDIIPLEVVLRNVIAGSFSK
                     RFGVEEGRVLETPIVEFYYKNDDLNDPFINDEHVKFLGIVNDEEIAYLKGETRRINEL
                     LKGWFAQIGLNLIDFKLEFGFDQEGTIILADEFSPDNCRLWDKNGNHMDKDVFRRDLG
                     NLTDVYQVVLEKLIAL"
     gene            5695..9420
                     /locus_tag="VC394_000978"
     CDS             5695..9420
                     /locus_tag="VC394_000978"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEP5994455.1"
                     /translation="MNKRIFVEKKADFGIKSASLVKELTHNLQLTSLKALRIVQVYDV
                     FNLAEDLLARAEKHIFSEQVTDCLLTETEITAELDKVAFFAIESLPGQFDQRAVSSQE
                     ALLLFGSDSQVKVNTAQLYLVNKDIAEAELEAVKNYLLNPVDSRFKDITLPLEEQAFS
                     VSDKTIPNLDFFETYKADDFTAYKVEQGLAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKNIDFSASKFQKQLQTTYDKYIAMRDELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITTPIAETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKENVVREKPEAGDVVILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRAGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLDKVPLKYQGLNGTEIAISESQERMSVVVRPSDVDAFIA
                     ACNKENIDAVVVATVTEKPNLVMTWNDETIVDLERRFLDTNGVRVVVDAKVVDKDLTV
                     PEARITSAETLEADTLKVLSDLNHASQKGLQTIFDSSVGRSTVNHPIGGRYQITPTES
                     SVQKLPVQHGVTTTASVMAQGYNAYIAEWSPYHGAAYAVIEATARLVATGADWSRARF
                     SYQEYFERMDKQAERFGQPVSALLGSIEAQIQLGLPSIGGKDSMSGTFEDLTVPPTLV
                     AFGVTTADSRKILSPEFKAAGENIYYIPGQAISEDIDFDLIKANFSQFEAIQTQHKIT
                     AASAVKYGGVLESFALMTFGNRIGASVEIAELDSSLTAQLGGFVFTSAEEIADAVKIG
                     QTQADFTVTVNGNDLAGASLLAAFEGKLEEVYPTEFEQTDVLEEVPAVVSDTVIKAKE
                     TIEKPVVYIPVFPGTNSEYDSAKAFEQVGASVNLVPFVTLNEVAIAESVDTMVANIAK
                     ANIIFFAGGFSAADEPDGSAKFIVNILLNEKVRAAIDSFIEKGGLIIGICNGFQALVK
                     SGLLPYGNFEEAGETSPTLFYNDANQHVAKMVETRIANTNSPWLAGVEVGDIHAIPVS
                     HGEGKFVVSASEFAELRDNGQIWSQYVDFDGQPSMDSKYNPNGSVNAIEGITSKNGQI
                     IGKMGHSERWEDGLFQNIPGNKDQTLFASAVKYFTGK"
     gene            9581..11035
                     /locus_tag="VC394_000979"
     CDS             9581..11035
                     /locus_tag="VC394_000979"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEP5994456.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPQAAQVTYFGLHSLQHRGQEGAGI
                     VSNDNGKLYGYRNVGLLSEVFKNQSELDNLTGNAAIGHVRYATAGSADIRNIQPFLYK
                     FHDGQFALCHNGNLTNAISLRKELEKQGAIFNASSDTEILMHLIRRSHNSSFMGKVKE
                     ALNTVKGGFAYLLMTENKLIAALDPNAFRPLSIGQMQNGAWVISSETCAFEVVGAKWV
                     RDVEPGEVILIDDRGIQCDRYTDETQLAICSMEYVYFARPDSTIHGVNVHTARKNMGK
                     RLAQEFKQDADIVIGVPNSSLSAAMGFAEESGLPNEMGLVKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMIDDSIVRGTTSRRIVGLLREAGASEVHVAIASPELKY
                     PCFYGIDIQTRRELISANHAADEVCDIIGADSLTYLSLDGLIESIGLETKAPNGGLCV
                     AYFDGHYPTPLYDYEEEYLRSLEEKTSFYIQKVK"
     gene            11063..12085
                     /locus_tag="VC394_000980"
     CDS             11063..12085
                     /locus_tag="VC394_000980"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEP5994457.1"
                     /translation="MSEKNAYAKSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVREPVLVSGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNNPVKFEEVVSGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQLIDGSKVKEGDILLGLASSGIHSNGYSLVRRVFADYTGKELLPELEGKQLK
                     DVLLEPTRIYVRAALPLIKEELINGIGHITGGGFIENVPRMFADDLAAEIDEDKVPVL
                     PIFKALEKYGDIKHEEMFEIFNMGVGLMLAVSPENVNRVKELLDEPVYEIGRIIKKAD
                     DSVVIK"
     gene            12253..12807
                     /gene="purN"
                     /locus_tag="VC394_000981"
     CDS             12253..12807
                     /gene="purN"
                     /locus_tag="VC394_000981"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEP5994458.1"
                     /translation="MKIAVFASGNGSNFQVIAEQFPVSFVFSDHRDAYVLERAQNLAI
                     PSFAFELKEFENKAAYEQAIVDLLDKHEIDLVCLTGYMKIVGETLLLAYEGRIINIHP
                     AYLPEFPGAHGIEDAWEAGVDQSGVTIHWVDSGVDTGQVIQQVRVPRLADDSLESFET
                     RIHETEYQLYPAVLDSLGVERKVI"
     gene            12991..14538
                     /gene="purH"
                     /locus_tag="VC394_000982"
     CDS             12991..14538
                     /gene="purH"
                     /locus_tag="VC394_000982"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEP5994459.1"
                     /translation="MTKRALISVSDKSGIVDFAKELKNLGWDIISTGGTKVALDNAGV
                     ETIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDADSHLQAAKDNNIELIDLVVVNL
                     YPFKETILRPDITYDLAVENIDIGGPSMLRSAAKNHASVTVVVDPADYATVLGELADA
                     GQTTFETRQRLAAKVFRHTAAYDALIAEYFTTQVGEAKPEKLTITYDLKQAMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDRPTVVVLKHM
                     NPCGIGQADDIETAWDYAYEADPVSIFGGIVVLNREVDAATAKKMHPIFLEIIIAPSY
                     SEEALAILTNKKKNLRILELPFDAQAASEVEAEYTGVVGGLLVQNQDVVAENPSDWQV
                     VTDRQPTEQEATALEFAWKAIKYVKSNGIIITNDHMTLGLGAGQTNRVGSVKIAIEQA
                     KDHLDGAVLASDAFFPFADNIEEIAAAGIKAIIQPGGSVRDQDSIDAANKHGLTMIFT
                     GVRHFRH"
     gene            complement(14596..15720)
                     /locus_tag="VC394_000983"
     CDS             complement(14596..15720)
                     /locus_tag="VC394_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEP5994460.1"
                     /translation="MKKFHRFLVSGVILLGFNGLVPTMPSTLISQQENLVHAAVLGDN
                     YPSKWKKGNGIDSWNMYIRQCTSFAAFRLSSANGFQLPKGYGNACTWGHIAKNQGYPV
                     NKTPSIGAIAWFDKNAYQSNAAYGHVAWVADIRGDTVTIEEYNYNAGQGPERYHKRQI
                     PKSQVSGYIHFKDLSSQTSHSYPRQLKHISQASFDPSGTYHFTTRLPVKGQTSIDSPD
                     LAYYEAGQSVYYDKVVTAGGYTWLSYLSFSGNRRYIPIKEPAQSVVQNDNTKPSIKVG
                     DTVTFPGVFRVDQLVNNLIVNKELAGGDPTPLNWIDPTPLDETDNQGKVLGDQILRVG
                     EYFTVTGSYKVLKIDQPSNGIYVQIGSRGTWVNADKANKL"
     gene            15973..17238
                     /gene="purD"
                     /locus_tag="VC394_000984"
     CDS             15973..17238
                     /gene="purD"
                     /locus_tag="VC394_000984"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEP5994461.1"
                     /translation="MKLLVVGSGGREHAIAKKLLASKGVDQVFVAPGNDGMTLDGLDL
                     VNIVVSEHSRLIAFAKENEISWAFIGPDDALAAGIVDDFNSAGLRAFGPTKAAAELEW
                     SKDFAKEIMVKYNVPTAAYGTFSDFEKAKAYIEEQGVPIVVKADGLALGKGVVVAETV
                     EQAVEAAQEMLLDNKFGDSGARVVIEEFLDGEEFSLFAFVNGDKFYIMPTAQDHKRAF
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVETIVRPVLEGMVAEGRPYLGVLYVGLILT
                     ADGPKVIEFNSRFGDPETQIILPRLTSDFAQNIDDIMMGIEPYITWQKDGVTLGVVVA
                     SEGYPLDYEKGVPLPEKTDGDIITYYAGAKFVENSELLLSNGGRVYMLVTTEDSVKAG
                     QDKIYTQLAQQDTTGLFYRNDIGSKAIRE"
     gene            17516..18007
                     /gene="purE"
                     /locus_tag="VC394_000985"
     CDS             17516..18007
                     /gene="purE"
                     /locus_tag="VC394_000985"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001875905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEP5994462.1"
                     /translation="MKTPIISIIMGSKSDWATMQKTAEVLDNFGIAYEKKVVSAHRTP
                     DLMFKHAEEARGRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGLDSLYS
                     IVQMPGGVPVATMAIGEAGATNAALTALRILSIEDQNLADALAHFHEEQGKIAEESSG
                     ELI"
     gene            17994..19067
                     /gene="purK"
                     /locus_tag="VC394_000986"
     CDS             17994..19067
                     /gene="purK"
                     /locus_tag="VC394_000986"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEP5994463.1"
                     /translation="MNSFKTIGIIGGGQLGQMMAIAAIYMGYKVITLDPASDCPASRV
                     SEVIVAPYDDVEALGQLAARCDVLTYEFENIDADGLDAVVSACQLPQGTDLLRISQNR
                     IFEKDFLANKAGVTVAPYKVVTSSLDLGGLDFTKTYVLKTATGGYDGHGQKVIRSAED
                     LPEAQQLANSAQCVLEEFVNFDLEISVIVSGNGEDVTVFPVQENIHCNNILSKTIVPA
                     RISDQLADKAKKMAVQIAKKLQLSGTLCVEMFATADDIIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPTIHLHAPAVMLNVLGQHVQQAIDYVAQNPSAHLHMYGKLE
                     AKHNRKMGHVTVFSDLPDEVEEF"
     gene            19069..20487
                     /locus_tag="VC394_000987"
     CDS             19069..20487
                     /locus_tag="VC394_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994464.1"
                     /translation="MYLKYIFKIKDRNLVSDYESLVRNLTINLQKLVIKDRTALIGDK
                     QLPITIYYRGENEKFPTGIVKVESDSVEGLELFDKTIKEIVGNESRFGKFTCIYDGIS
                     TYYSTEIYSELHEIERRTQEFLREAFFLSDKSGELKEYQATIKKMDSSDFSFSKLINE
                     IFKASDITDTFIDHVRACVEFGTVPNGDLLPKSFWDSLDDSLLDDKEQGLEINNLLNE
                     IRSTRNTIAHCKEFKKKDYINCIEQVKKLKQALDKIIASIESTDISKMSREVSRINNS
                     LQADNDYIKQKYFIEYDIFEKDILARTGLLEIDWENVSDDIEPLKIHRNFYESISEKT
                     DSEVIEEIKNLEDINFTDEKGNDLDDLTIIVPAQDEGFKRVFLGQREWYDIRIGKSKR
                     NRIKYIAGYEVAPESGVKYWAEVDKIVPSDNYVGYWKVIFKGEVKEYSEYKRLGNTWP
                     PQNSRYVSKKALDEAKYLSDVF"
     gene            20507..21799
                     /gene="purB"
                     /locus_tag="VC394_000988"
     CDS             20507..21799
                     /gene="purB"
                     /locus_tag="VC394_000988"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEP5994465.1"
                     /translation="MIERYSRPEMAAIWTEENKYRAWLEVEILADEAWAELGEIPKED
                     VAKIREKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIVADKAREHKMTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRPQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMVTAYENVSLWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMMRNMESTFGLIYSQRVMLKLIEKGMTREEAYDLVQPKTAYSWDNQVDFKPL
                     LEEDTKVTSCLTQEELDELFNPIYYTKRVDDIFKRLGI"
     gene            21930..>22404
                     /gene="rgg4"
                     /locus_tag="VC394_000989"
     CDS             21930..>22404
                     /gene="rgg4"
                     /locus_tag="VC394_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Rgg4/ComR"
                     /protein_id="HEP5994466.1"
                     /translation="MLEHFGGKVKVLRLEKRISREDLCGDESELSVRQLARIELGQSI
                     PSLSKVIFIAKALNVSVGYLTDGADLELPKRYKELKYLILRTPTYMDDGKLQVREEQF
                     DEIFEDYYDKLPEEEKIIIDCLQATLDTLLSENTNFGIDLLQEYFNQIKTKVRFRQ"
BASE COUNT         6755 a         4065 c         5078 g         6506 t
ORIGIN      
        1 aggagtagtt tttttattat gaaaaaaaga attttatcag cagttcttgt aagtggtgtt
       61 accctcggag cagctacaac tgtaggagcg gaggatttaa gtactaagat tgctaagcaa
      121 gattctatta tctcaaatct gactacagag caaaaagctg cacagaatca agtttcagcg
      181 ttacaggctc aagtaagttc actacaatct gaacaagata aactgaccgc aagaaataca
      241 gaacttgagg cgctttcaaa gcgatttgag caagaaatta aggctctaac aagtcaaatt
      301 gttgctcgta atgaaaaatt aaaaaatcaa gctcgtagtg cttataaaaa caatgaaact
      361 tctggttata ttaatgcact tttgaattct aaatcaattt ctgatgttgt aaaccgttta
      421 gtagcaatta atagagctgt ctctgctaac gctaaattgt tagaacaaca aaaagctgat
      481 aaagtttccc ttgaagaaaa gcaagctgct aaccaaacag ctattaatac cattgccgct
      541 aatatggcaa tggctgaaga aaaccaaaat acattacgta ctcaacaagc taatttggta
      601 gctgcaactg caaatttagc tctccaatta gcatctgcta ctgaagataa agctaatttg
      661 gtagctcaaa aagaagctgc agaaaaagct gctgctgaag ccttagcaca agaacaggct
      721 gctaaagtta aggcacaaga acaggctgca caacaagcag catctgttga agcagcaaaa
      781 tctgctatta ctccagcacc acaagctact ccggcagcgc aaagtagtaa tgctattgaa
      841 ccagctgcac tcacggctcc ggcagctcct tctgcaggac cacaaacatc atatgattct
      901 tctaatactt atccagttgg acaatgcaca tggggagcta aatctttagc tccttgggca
      961 ggaaataatt ggggaaatgg tggtcaatgg gcttatagtg ctcaagcagc tggttatcgt
     1021 actggttcaa cgccgatggt aggtgcgatt gccgtttgga acgatggtgg ttatggacat
     1081 gtcgccgttg tagttgaggt tcaaagtgcc tcaagtattc gtgtgatgga gtctaactac
     1141 agtggtagac agtacattgc tgaccaccgt ggctggttta atccaacagg tgttacattt
     1201 atttatccac actaacatat tatttaggtt agaacagttg ttctaacctt tttgattact
     1261 cctattgtgg aatagttctg agagtattga cgatatagtt aatttataag agagttgttt
     1321 ggccaatcgc ttatgtataa tataagagaa gtatataaag tcgataaaag gtatatttaa
     1381 tacattcttt aacggttttt caatttcatt tgataattca cttgaaaagc gttaaaatgg
     1441 tataggatta aaactttgga ggaaaaaatg tcttattctg atttaaaatt gtttgcttta
     1501 tcgtcaaaca aggaattagc agaaaaagtt gcgtctgcaa tggatcttca acttggaaag
     1561 tctacggttc gccagttttc agatggtgaa atccaagtaa atattgaaga atcaattcgt
     1621 ggtcatcatg tctttatttt gcaatcaact agctcacctg tcaatgacaa tttaatggaa
     1681 attttgatta tggtcgatgc tttaaagcgt gctagtgctg aaaaaatttc agttgtgatg
     1741 ccttactacg gctatgcacg ccaagaccgt aaagcgcgtt ctcgtgaacc tattacttcg
     1801 aaattagttg ctaatatgtt agaagttgct ggtgtagatc gcttattaac ggttgattta
     1861 catgctgctc aaattcaagg gttctttgat attccagttg atcacttgat gggtgcacct
     1921 ttaattgcag actattttga tcgtcatggt ttagtgggtg aagatgttgt cgttgttagt
     1981 cctgaccatg gtggtgtgac tcgtgcgcgt aaattagcac aattcctcca aacaccaatt
     2041 gcaattatcg ataagcgtcg tagtgtggat aagatgaaca ccagtgaagt gatgaatatt
     2101 atcggaaatg tttcgggcaa gaaatgtatc ttgattgacg atatgattga taccgcaggg
     2161 actatttgcc atgcagcaga tgcgcttgct gaagcggggg caacagctgt gtatgcgtca
     2221 tgtacgcatc ccgttctttc tggaccagca cttgataata ttcaacgttc ggctatcgaa
     2281 aaactaattg ttcttgatac tatttatctg ccaaaagaac gccttattga taaaattgaa
     2341 caaatttcaa ttgctgattt ggttgcagaa gcgattattc gcattcatga aaaacgtccg
     2401 ttgtcacctt tatttgagat gggcaattaa acttaacgaa gcctgagata atagatctca
     2461 ggctttttga cttgaataaa atgtaagggt aatgtggtat taattattgt gggatgaact
     2521 atgcaattct aaaggatttt gggtataata agaaagataa caacagttta ttgtaaacta
     2581 agataaaagg taagagaaaa atacgacgag gtaatatgca actaacagaa tcactaggca
     2641 tcgttctttt taataggaat tatcgagaag atgataaatt agtcaaaata tttactgaag
     2701 tagcaggtaa gcagatgttt ttcgtgaaac atattagtcg ttccaaaatg tcctcaatca
     2761 ttcaaccact aacgattgct gattttattt tcaagttaaa tgatacaggc ttgtcttatg
     2821 ttgttgacta tagtaacgtt aacacttatc ggtatattaa taatgatatt tttcgattag
     2881 cctatgctag ttatgtctta gcattagctg atgctgcgat tgcagataat gaatcagatt
     2941 cgcatttgtt tacgtttttg aaaaaaacac ttgatttgat ggaagagggc ctagattatg
     3001 aaattttgac aaatattttt gaaattcaga tattagatcg ttttggtatt agtctaaact
     3061 ttcatgagtg tgccatttgt catcgcactg atttaccgct tgatttttcc catcgttttt
     3121 cagctgtact ttgttctgaa cattattaca aagacaaccg acgtaatcat ttagatccaa
     3181 atgttatcta cttgttgagt cgatttcaaa aaatcacatt tgatgatttg agaactattt
     3241 cattgaataa agacatcaaa aagaagcttc gtcagttcat tgatgagttg tatcacgact
     3301 atgtaggaat caaattaaaa agtaaaacat tcattgataa tttagttaag tggggagata
     3361 ttatgaaatg atagggcctc tttcgtttat gggaattggt ttcagaatat gctataataa
     3421 aaatactatt aatagattgt gtaagaacac aagatataat taaaggagtg gaaatgaaga
     3481 gaattgcaat agatgctatg ggtggagata acgctcctaa agctattgtt gagggtgtta
     3541 atcaagcaat agaagcattt tcagatatcg aaattcaact ttacggtgat cagacaaaga
     3601 ttaactccta tcttattcaa tcagatcgtg ttgctattat tcatacagat gaaaaaatta
     3661 tgtcagatga tgaacctgca aaagctgttc gccgtaaaaa gaaggcttca atggttttag
     3721 cagctaaggc agtgaaagaa ggaaaagcag atgccattat ttcagcaggt aatacaggtg
     3781 ctcttttagc tgtaggtttg tttgttgtgg ggcgtatcaa gggagttgat cgtccaggat
     3841 tattatcaac tattcccaca gtaaccggat taggatttga tatgttggat cttggagcta
     3901 atgctgaaaa tacggcaaaa catttacatc aatatgctat tttaggctct ttctatgcta
     3961 agaatgtacg tgggattgct aatccacgtg ttggtctatt aaacaatggt acagaagaaa
     4021 caaaaggaga tccccttcgt aaggccactt atgaactttt aacagcagat aatacaatat
     4081 cttttgttgg taatgtcgag gcgcgagaac ttatgtcagg tgttgcagat gttattgtat
     4141 cagatggttt cacgggaaac gcagtgttga aatcaataga aggaacagct attagcatta
     4201 tggggcaact gaaacagata attaatagcg gcggtattaa gactaaaatt ggagctagtc
     4261 tacttaaaag tagtctttac gagatgagga agactcttga ctactctagt gctggaggtg
     4321 cagttttatt tggtttaaag gcacctgttg taaagtctca tggttctagt gatgtaaaag
     4381 ctattttttc aacgataaaa caagtacgta cgatgctaga cactaacgtg gtcggacaat
     4441 tagtagaaga atttgctaag gagactcaag taaatgacta gacaggagat ttttgaaaga
     4501 ctgattaact taattcagaa gcaaagaagc tatttgagtg tagctattac agaacaaaca
     4561 catttaaaaa atgatttagc ggttgactct attgaactag ttgaatttat tattaatgtt
     4621 gaagatgaat ttcatcttgc tattcctgat gaagatgttg aggatatggt atttatggga
     4681 gacgttttag attatttagt tcagcgtttg acttagttag aataataaaa agagcgtaag
     4741 ctctttttat tattctttaa aaacgaacct ttttttaaaa aaacttataa acgtaaataa
     4801 tatcttgaat atttttatag agtatgttac tcttaaatga aaacagaaag aaaggcgaat
     4861 aatcaagtga ctaaccagct tatttataaa ggaaaagcga aagatattta tagtacaaaa
     4921 gatgaaaatg tgattagaac ggtctataaa gaccaagcta ctatgttgaa tggagctcgt
     4981 aaggagacta ttgatggcaa gggagcacta aataatcaga tttcatctct tatttttgag
     5041 aaattaaata aggctggtgt ggtgacacat tatattgagc aaatttcaaa aaacgaacaa
     5101 ttgaataaaa aagttgatat tattccactt gaggtggtgt tacggaatgt gatagcaggc
     5161 tcattttcaa aacgttttgg agtggaagaa ggacgtgttc ttgaaacgcc aattgtggaa
     5221 ttttactaca aaaatgatga tctgaatgat ccttttatca atgatgagca tgtgaagttt
     5281 ttgggtattg ttaatgatga ggagattgct tatctgaaag gagagactcg ccgtattaat
     5341 gaactcttga aaggttggtt tgctcagatt gggcttaact tgattgactt caagttggag
     5401 ttcggatttg atcaagaagg tacaattatc ttagcagatg aattttcgcc tgataactgt
     5461 cgcctatggg ataagaatgg taaccatatg gacaaggatg tttttagaag agaccttggc
     5521 aacctgacgg atgtctatca agttgtactt gagaagttga tagcattgta actagaagag
     5581 gaaggaattg aatccgttcg ggaaactccg aaaaaaagat ggaacttact gaaatctcaa
     5641 tttcagatgt cctcctattt tccagtcgct cccttgtcgg acttggtatc ttatatgaat
     5701 aaacgtattt ttgttgagaa aaaggctgac tttggtatta aatcggctag ccttgtgaaa
     5761 gagttgacgc ataatctaca actgacttct ttgaaggctt tgcgtattgt gcaggtctat
     5821 gatgtcttca atttggctga ggatttgctg gcgcgtgctg agaagcatat tttctctgag
     5881 caggtgacag actgtctttt gacggaaact gaaatcactg cggagcttga taaggttgcc
     5941 ttctttgcca ttgagtcgct tcctggtcaa tttgaccaac gtgctgttag ttcgcaagaa
     6001 gctttgctat tatttggaag tgacagtcag gttaaggtca atacagccca gctttacttg
     6061 gtcaataagg atattgcaga agcagagctt gaagccgtta agaactatct tttgaaccct
     6121 gttgattcgc gtttcaagga cattactttg ccgcttgaag agcaggcttt ctctgtgtct
     6181 gataagacga tccctaatct tgatttcttc gagacttata aggctgacga ttttacggct
     6241 tataaggtag agcagggctt ggctatggag gtcgatgatc ttctcttcat ccaagattat
     6301 ttcaaatcaa tcgggcgtgt gccaactgag actgagttga aagttttgga tacgtattgg
     6361 tcagaccact gtcgtcacac aacctttgaa actgaattga agaacattga tttttcagct
     6421 tctaaattcc aaaaacaatt gcagacaact tatgacaaat atatcgccat gcgtgatgag
     6481 cttggtcgtt ctgaaaagcc acaaacactt atggatatgg cgactatttt tggtcgttat
     6541 gagcgtgcca acggtcgtct ggacgatatg gaagtctcag atgaaatcaa tgcctgctca
     6601 gttgagattg aagtagatgt tgatggtgtg aaagagcctt ggctcctcat gtttaagaat
     6661 gagacacaca atcacccaac agaaattgag ccattcggtg gagcggcgac ttgtatcggt
     6721 ggtgctattc gtgacccatt gtcaggacgt tcatacgttt atcaggctat gcgtatttca
     6781 ggcgcaggcg atatcacgac tccgattgcg gaaacacgtg ctggtaaatt gccacaacag
     6841 gttatttcta agactgcggc gcacggctat tcttcatatg gtaaccaaat tggacttgcg
     6901 acaacttatg tgcgtgagta cttccaccct ggcttcgtag ccaaacgtat ggagcttgga
     6961 gctgtggttg gtgctgcacc taaggaaaat gtggttcgtg aaaaaccaga agcaggtgat
     7021 gtggtaatct tgcttggtgg taaaacaggg cgtgacggtg tcggcggtgc gacaggttca
     7081 tctaaggttc aaacggttga atctgtggaa acagcaggag cagaggtaca aaaagggaat
     7141 gccatcgaag agcgtaagat tcaacgtctt ttccgtgctg gcaatgtcac tcgtctgatc
     7201 aagaaatcaa atgacttcgg tgcaggtggt gtctgtgttg ccatcggtga attggctgac
     7261 ggtcttgaaa tcgatttgga caaggtgcct cttaaatacc aaggtcttaa tggtactgaa
     7321 attgcaatct cagaatctca agagcgtatg tcagtcgttg ttcgtccaag tgatgtggat
     7381 gccttcatcg cagcctgcaa caaggaaaat atcgatgcag tcgtagttgc gactgtgact
     7441 gaaaaaccaa atcttgtcat gacttggaat gatgaaacaa ttgttgattt ggaacgccgt
     7501 ttccttgata ccaatggtgt ccgtgtcgtt gttgatgcta aagtcgttga caaggacttg
     7561 acagttccag aagcacgcat aacatcagca gagacacttg aagcagatac gcttaaggtc
     7621 ttgtctgacc tcaaccatgc tagtcaaaaa ggtctccaaa ccatctttga ctcatctgtt
     7681 ggtcgttcaa ccgttaacca cccaatcggt ggtcgttacc aaatcacacc gacagaaagt
     7741 tctgttcaaa aattgccagt tcaacatggt gtgacaacaa ctgcatctgt tatggctcaa
     7801 ggttacaatg cttatattgc agagtggtca ccttatcacg gtgctgccta tgctgttatt
     7861 gaagcgacag ctcgcttggt agcaacaggt gctgactggt ctcgtgcacg tttctcttac
     7921 caagagtatt tcgagcgtat ggataaacag gcagagcgtt ttggtcagcc agtatcagct
     7981 cttcttggtt ctattgaggc tcagattcaa cttggcttgc catcaatcgg tggtaaggac
     8041 tctatgtctg gtactttcga agacttgaca gtaccaccaa ccttggtagc tttcggtgtg
     8101 acaacagcgg acagccgcaa gattctctct cctgagttta aagcggctgg tgagaatatc
     8161 tactacattc caggtcaagc tatttcagaa gatattgatt ttgaccttat caaggctaac
     8221 ttcagtcagt ttgaagctat tcaaactcaa cataagatta cagctgcctc agccgttaaa
     8281 tacggtggtg tcctagaaag ttttgctctc atgacttttg gtaaccgtat cggtgcttct
     8341 gttgaaattg cagagcttga cagcagcttg acagctcaac tcggaggttt tgtctttaca
     8401 tcagctgagg aaattgctga cgcggtgaaa attggtcaaa ctcaggcaga ctttacagtc
     8461 actgtcaatg gaaatgacct tgctggcgct agccttctag cagccttcga aggcaaattg
     8521 gaagaggttt acccaacaga atttgaacag acagatgttc ttgaagaagt tcctgctgtg
     8581 gtatcagata ctgttatcaa ggctaaggaa acaattgaaa aaccagtggt ttacattcca
     8641 gtcttccctg gtaccaactc agagtacgat tcagctaagg cctttgaaca ggttggagct
     8701 agtgtcaact tggtaccatt tgtaaccttg aatgaggttg ctattgctga gtcagttgac
     8761 actatggttg ctaatattgc taaggcaaat atcatcttct ttgctggagg tttctcagca
     8821 gcggatgaac cagatgggtc tgctaagttt atcgtcaata tcttgcttaa cgagaaggtc
     8881 cgcgcagcta ttgacagctt catcgaaaaa ggtggcctta tcatcggtat ctgtaatggt
     8941 ttccaagccc ttgttaaatc aggtcttctt ccatacggaa acttcgagga agctggtgag
     9001 acaagtccaa ctctcttcta taacgatgct aatcagcatg ttgccaagat ggttgagact
     9061 cgtatcgcaa ataccaactc accttggttg gcaggagttg aggtcggcga tattcatgcc
     9121 attccagttt cacatggtga aggtaaattt gttgtcagcg cttctgaatt tgcagagcta
     9181 agagacaatg gtcaaatctg gagccaatat gtggactttg acggacaacc atctatggat
     9241 tctaaataca atccaaacgg ctctgtcaat gccatcgaag ggattaccag caagaatggt
     9301 caaatcatcg gtaagatggg acactcagaa cgctgggaag acggactctt ccaaaatatt
     9361 cctggtaaca aagaccaaac cctctttgca agtgctgtaa aatactttac agggaagtaa
     9421 aaaggttcag tgaacctttt tagccctcgc cttgaattag tgaagggcaa ggttagaaag
     9481 aatctcgctt aatttttgag aaatagtaaa cacgggcgat aagttgtgcg aaaaactttc
     9541 tattttcgct ttacttgaca gcctttgtat cgaggtaaaa atgacatacg aagtaaaatc
     9601 tctaaatgaa gaatgtggag tctttggtat ctggggacat cctcaggcag ctcaagtcac
     9661 ttactttggg cttcatagcc ttcaacatcg cggtcaagaa ggagctggta ttgtttcaaa
     9721 tgacaatggg aaactctatg gttatcgaaa tgttgggctc ctttctgaag tttttaagaa
     9781 tcaatctgaa ttagataatt taactgggaa tgcggctatt ggacatgttc ggtatgctac
     9841 tgcaggttct gcagatattc gcaatattca gccttttctt tataaatttc atgacgggca
     9901 atttgcttta tgccataatg gtaatttgac aaatgctatt tccttaagga aagaattaga
     9961 aaagcaaggt gcaattttca atgcctcctc agatactgaa attttgatgc acttgattcg
    10021 tcgaagccat aactcaagtt tcatgggaaa ggtaaaagaa gctctaaata cagtaaaggg
    10081 tggctttgcc tatctactga tgacagaaaa taaactcatt gctgctcttg accctaatgc
    10141 ctttcgtcct ttgtcaattg gacaaatgca aaatggtgcc tgggttattt ccagtgagac
    10201 ctgtgctttt gaggtggtag gcgcaaaatg ggttagagat gttgaacctg gcgaagttat
    10261 tcttatagac gatcgtggta ttcaatgtga tcgttatact gatgaaacgc aactggcaat
    10321 ttgttcgatg gaatatgtct attttgcaag gccggattca actatacatg gtgttaatgt
    10381 tcatacggct cgaaaaaata tgggaaagcg tcttgcacaa gaatttaaac aggatgctga
    10441 tattgtaatt ggtgtcccaa attcgtcctt atcggctgct atgggctttg ctgaagaatc
    10501 cggattacca aatgagatgg gtcttgtaaa aaatcagtat acgcagcgaa cctttattca
    10561 accgacacaa gaattaaggg aacaaggtgt tcgaatgaaa ctatcagcgg tatctggtgt
    10621 tgtcaaagga aagcgcgttg ttatgattga tgactcaatt gtaagaggaa cgacttctag
    10681 aaggattgtc ggattattaa gagaagcagg agctagtgaa gtacatgttg ctatagctag
    10741 tccagaatta aagtatcctt gtttttatgg tattgatatt caaacaagac gagaattaat
    10801 ttcagcaaat cacgctgctg acgaagtgtg tgatattatt ggcgcagata gtctgaccta
    10861 tctctcacta gatgggttga ttgaatcaat tggactcgaa acaaaagcac caaatggtgg
    10921 tttatgcgta gcttactttg atggacatta tccaacaccg ctttatgatt atgaagaaga
    10981 atacctcaga agtttagaag aaaaaacaag tttttatatt caaaaagtga aataagctct
    11041 gctaattata taaggagaaa acatgtctga aaaaaatgct tatgcaaaat ctggtgttga
    11101 tgttgaagct ggttatgaag tggttgaaag aataaaaaaa cacgttgctc gtacagaacg
    11161 tgcgggagtc atgggagctc taggtggctt tggtgggatg tttgacttga gtaaaacagg
    11221 agttagagag cctgttttgg tctcagggac agacggtgtt ggcacaaaac tcatgcttgc
    11281 tatcaagtac gacaagcacg acacaatcgg tcaagactgt gttgccatgt gtgtcaatga
    11341 tattattgca gcaggtgctg agccccttta cttccttgac tacgtagcga caggaaaaaa
    11401 taatccagtc aaatttgaag aagttgtttc tggcgttgca gaaggttgtg ttcaagcagg
    11461 agcagcactc attggaggtg agaccgctga aatgcctggg atgtatggcg aagatgatta
    11521 tgaccttgct ggctttgctg ttggtgtggc tgaaaaatct cagctcatcg acggttcaaa
    11581 agtaaaggaa ggcgatattc ttttgggact tgcttcaagt ggtatccatt caaacggtta
    11641 ttctttggta cgtcgtgtct ttgctgacta tactggtaaa gagctgcttc cagaacttga
    11701 aggaaaacaa cttaaggatg tccttcttga gccaactcgt atttatgtta gagcagctct
    11761 gccattaatc aaggaagaac taatcaacgg tatcggccac atcacaggtg gtggctttat
    11821 cgagaatgtt cctcgtatgt ttgcagatga tttggctgcg gaaatcgatg aggacaaggt
    11881 gccagtgctt ccgattttca aggcgcttga aaaatatggt gacatcaagc acgaagaaat
    11941 gtttgaaatc ttcaatatgg gtgtcggtct tatgctagct gttagtcctg aaaatgttaa
    12001 ccgcgtcaaa gagctcttgg atgaaccagt ttatgaaatc ggtcgtatca tcaagaaagc
    12061 agacgatagt gtggtgatta aataagtcac taaattttat aagaaaggag tgtttagtta
    12121 gtttgcctaa ctgaacacgg gattaaattt ctaggaaaaa agataaaatg acttagacgc
    12181 aagcgtcgtt gttcgttttc ctatttttct acgaaatttt tcggcaagcc gaatcatccc
    12241 tttgtatcga atatgaaaat cgctgttttt gcttctggta atggttccaa ctttcaggtc
    12301 atagcagagc agtttccagt tagttttgtc ttttcagatc atcgtgatgc ctatgtttta
    12361 gagcgtgctc agaatttagc cattccaagc ttcgcttttg aactcaagga gtttgagaat
    12421 aaggcggctt acgagcaagc catcgttgat ttgttggaca aacacgagat tgatttggtc
    12481 tgtctaacgg gctatatgaa gattgttgga gaaaccttac tcttggccta tgaggggcgt
    12541 attatcaata ttcacccagc ctacctgcct gaatttccag gtgcccacgg tatcgaggat
    12601 gcttgggaag ctggtgttga ccagtctggc gtgaccatcc actgggttga ctctggtgtg
    12661 gataccggtc aggtcatcca acaagtgcgc gtgccacgcc tagcagacga tagcctagaa
    12721 agctttgaaa cccgtatcca tgaaaccgaa tatcaactct acccagctgt cttagatagc
    12781 ttgggagtgg agaggaaagt aatctagcct ttatcttaaa attttaggta aagttaggaa
    12841 agatagtaaa gaagaattaa cgttccaaaa agaaaggaat tgaacccgtc tggaaggcac
    12901 agaaaaaaga taaacatctc ctaggcgcaa gcgtcttcgt cgagtttcct atttttcctt
    12961 ggccttctag tcggacttgg tatcttaaat atgactaaac gtgctttaat ctcagtgtct
    13021 gacaagtcag gaattgttga ctttgcaaaa gaattgaaaa acttgggttg ggatattatc
    13081 tcaactggtg ggactaaggt tgcccttgat aatgctggcg ttgagaccat tgccatcgac
    13141 gatgtgactg gattcccaga gatgatggac ggtcgtgtta agaccctcca cccaaacatt
    13201 cacggtgggc ttttggctcg tcgcgacgct gacagccacc ttcaggctgc taaggacaac
    13261 aatatcgagc tgattgacct cgtggttgtc aacctctatc ccttcaagga gactatcctt
    13321 cgtccagata taacctatga tttggcggtg gaaaacatcg acatcggcgg tccatcaatg
    13381 cttcgctcag ccgctaaaaa tcacgctagc gtgaccgttg tggttgaccc agcggattat
    13441 gcgactgttt tgggagaatt ggctgacgct ggtcagacga catttgaaac gcgtcaacgc
    13501 ttggcagcta aggtctttcg tcacacagca gcctacgacg ctttgattgc tgagtacttc
    13561 acaactcaag tgggagaggc taagcctgaa aaattaacca tcacttacga ccttaaacag
    13621 gctatgcgtt acggagaaaa tccacaacaa gacgctgatt tctatcaaaa agccttgcca
    13681 acagactact caattgcttc tgctaaacag ctcaacggaa aagaattgtc cttcaataat
    13741 atccgtgatg ccgatgcagc aatccgtatt atccgtgatt tcaaagatcg tccaactgtt
    13801 gttgtcctca aacacatgaa cccatgtggt atcggacagg ctgatgatat tgagacagct
    13861 tgggattacg cttatgaagc tgacccagtt tcaatctttg gtggaattgt cgtccttaac
    13921 cgtgaagttg acgcagcgac agctaagaag atgcacccta tcttcttgga aatcatcatc
    13981 gcaccatcat actcagaaga agcgctagct attctcacaa ataaaaagaa aaacttgcga
    14041 atccttgagt tgccgtttga tgcccaagct gccagcgaag tggaagctga gtacactggc
    14101 gtagttggtg gacttttggt gcaaaaccaa gacgttgtgg ctgaaaatcc atctgactgg
    14161 caagtggtga cagaccgcca accaacagaa caagaggcga ctgcccttga gtttgcctgg
    14221 aaggctatca agtatgttaa gtctaacggg attattatta ctaacgatca catgacgctt
    14281 ggactcggtg caggtcaaac caaccgtgtc ggctcagtca agattgctat cgagcaggct
    14341 aaagaccacc ttgacggtgc cgttctagca tcagatgcct tcttcccatt tgcggacaac
    14401 attgaagaaa tcgctgccgc agggatcaaa gcaatcatcc aaccaggtgg ttcagttcgt
    14461 gaccaagact ctattgacgc cgcaaacaaa catggcttga ccatgatctt cacaggtgtg
    14521 agacatttta ggcattaata aaaaaatgtg gtctaattaa aaccacattt tttattaaaa
    14581 gaaaatataa gttaactata atttgttagc tttatcagca tttacccatg ttccacgaga
    14641 tccgatttga acataaatac cattacttgg ttgatcaatt tttaatactt tataactacc
    14701 agtgacggta aaatattcac ccacacggag aatttgatct cctaaaactt ttccttggtt
    14761 atctgtttca tctaatggtg tgggatcaat ccagtttagt ggagttgggt ctcctccggc
    14821 taattcttta ttaacgatca aattattaac aagctgatct acacgaaaaa cgccagggaa
    14881 ggtaacagta tcaccgacct taatggaagg ttttgtattg tcattttgaa ccacagactg
    14941 tgcgggctct ttaatgggaa tatagcgtcg gtttccagaa aaactgaggt agctaagcca
    15001 tgtataacct ccagcagtca cgactttatc gtaataaaca gattgacctg cttcatagta
    15061 agcaagatca gggctatcga tactggtttg tcctttgact ggtaatctgg ttgtaaagtg
    15121 ataagttcca gaggggtcaa atgaagcttg agaaatgtgt tttagttgtc ttgggtagga
    15181 atgacttgtc tgagatgata agtctttaaa atggatataa ccacttacct gagattttgg
    15241 aatttgacgc ttatggtatc tttcagggcc ttgtccagcg ttgtaattat actcttcgat
    15301 agtgacagtg tctccacgga tatcagctac ccatgctaca tgaccgtaag cagcatttga
    15361 ctgataagcg tttttatcaa accaagcgat agcccctatg cttggtgtct tattcacagg
    15421 ataaccctga tttttcgcga tatgtcccca cgtgcaggca ttaccgtagc ctttaggtaa
    15481 ctgaaaacca ttagcagagc ttaaacgaaa agctgcaaaa gaagtgcatt ggcggatata
    15541 catgttccac gaatcgattc cattgccttt tttccactta ctcggatagt tatcgcctaa
    15601 aacagctgca tgaacaagat tttcctgttg cgaaataagt gtagatggca tagtaggtac
    15661 tagaccatta aaacctaaaa ggattactcc tgagaccaaa aaacgatgaa attttttcat
    15721 taattacctc ctgtaactta ttattcgaaa gcaagaaaca aaaaatgtta tttttagatg
    15781 attattttga aaattggctt tacttgatat taaataactg tttgattggc taatagagtg
    15841 ttccttattt caagaattgt ggtctactat cacattttta caatgtttca attattgata
    15901 actaatacat tatgtaaaaa agcgaacgtt tatgatataa tatctaatta taatacgaaa
    15961 gaggtggatg ttttgaaatt acttgttgtt ggttcaggtg gtcgtgaaca tgcgattgct
    16021 aagaagttgt tagcgtctaa gggtgtggat caggtttttg tggcacctgg taatgatggt
    16081 atgaccttgg atggtctgga cttggtgaat atagtagttt ccgaacattc caggttgatt
    16141 gcctttgcta aggagaatga gatttcttgg gcctttattg gtcctgatga tgcgctagca
    16201 gctggtatcg ttgatgattt caacagtgct ggactcagag ctttcggtcc aacaaaggca
    16261 gcagcggagc tagagtggtc aaaagacttt gccaaggaaa tcatggtcaa atacaatgtc
    16321 ccaacagcag cctatggcac attttcagac tttgaaaaag ccaaagccta catcgaagag
    16381 cagggcgtac ctatcgtggt caaggctgat ggcttggcgc taggtaaggg tgtggtcgtg
    16441 gctgaaaccg ttgagcaggc ggtagaggcg gcgcaagaga tgcttctgga caataagttt
    16501 ggcgattcag gagcgcgtgt ggttatcgag gaattcttgg atggcgagga gttttccctc
    16561 tttgcctttg ttaatggtga taagttctac atcatgccga cagctcagga tcacaagcgt
    16621 gcctttgacg gagacaaggg gccaaacact ggtggtatgg gtgcctatgc gccagttcct
    16681 catctacctc agagcgtggt agacacagcg gttgagacga tcgttaggcc tgtccttgag
    16741 ggcatggttg ccgaagggcg tccttatcta ggtgtcctct atgtggggct tatcctgaca
    16801 gctgatggac ctaaggttat cgagttcaac tcacgtttcg gtgaccctga aactcagatt
    16861 atcctccctc gcctgacttc cgatttcgct cagaatattg acgatatcat gatgggcatt
    16921 gagccttaca tcacttggca gaaggacggt gtgactttgg gtgttgtcgt tgcctcagaa
    16981 ggctatccac tcgattatga gaaaggtgtg ccactgcctg aaaagaccga cggcgatatc
    17041 atcacctact atgccggagc taagtttgtg gaaaatagcg agctacttct ctcaaacgga
    17101 ggacgtgtct atatgcttgt caccacagaa gacagcgtca aagcagggca ggacaaaatc
    17161 tatacccagc tagcccaaca agacacaaca ggtctcttct accgaaatga catcggaagc
    17221 aaagctataa gagaataaga ctaaccattc aaatattagc gtcagcaatc aacgaccgaa
    17281 ggtcgctcca aaaagaaaga agaaaggaac tgaacacggg actaatttcc taggaaaaaa
    17341 ataaaactac ctggacacaa gcgtcatcgt tagttttctg cgtcgtaggc gtctgatgct
    17401 aaagcaccta gtcgccctga cgtgggttcg tcttcgaaat caaagatttc ggacttaccg
    17461 catatttttc tacggaaatt ttcgacaagt cgaaacgccc ctttgtatct taattatgaa
    17521 aactccaatt atttctatta tcatgggctc caaatctgac tgggcaacca tgcaaaaaac
    17581 ggctgaagtt ctagacaact ttggcattgc ttacgagaag aaagtcgtct ctgcccaccg
    17641 tacgccagac ctcatgttca agcatgctga ggaagcacgt ggtcgtggca tcaaaatcat
    17701 catcgcagga gcaggtgggg cagctcactt gcctggtatg gtggcagcta aaacaaccct
    17761 gcctgttatc ggtgtgcctg tcaaatcacg tgccctttca ggtttggatt cactctattc
    17821 gattgtgcag atgcctggcg gtgtgcctgt ggcaaccatg gctatcggag aagcgggagc
    17881 gactaacgct gccctgacag ccctccgtat tctctccatc gaggatcaaa atctggctga
    17941 tgcgctagct catttccatg aggaacaagg aaaaatcgca gaggagtcaa gtggtgaact
    18001 catttaagac cattgggatt atcggtggtg gtcagctggg gcagatgatg gccattgcgg
    18061 ctatctatat gggctacaag gtcatcacgc tggatcctgc tagcgactgc cctgcctccc
    18121 gcgttagcga ggtaattgtg gcgccttacg atgatgttga ggctttggga caattggctg
    18181 cgcgttgcga tgtattgact tatgagtttg agaatattga tgcggacggt ttggatgccg
    18241 ttgtgtcagc ttgtcagcta ccgcagggga ccgatctgct ccgcatttcc caaaaccgta
    18301 tctttgaaaa agactttctg gcaaataagg ctggtgtgac tgttgctccc tataaggtgg
    18361 tgacgtctag ccttgaccta gggggacttg attttaccaa gacctatgtc ctcaagacag
    18421 cgacaggtgg ttatgacggt catgggcaaa aggttatccg ctcagcagaa gatttgccag
    18481 aggcgcagca attagctaac tcagctcagt gtgtcttgga agagtttgtc aactttgacc
    18541 ttgaaatctc agtcatcgtg tctggaaatg gagaggatgt gacggtcttt cccgttcagg
    18601 aaaatatcca ctgcaacaat atcctgtcaa aaactatcgt gccagctcgt atctcagatc
    18661 aactggctga taaggctaag aaaatggcgg tgcagattgc caagaaactc cagctgtcag
    18721 gaactctctg tgtggaaatg tttgcgaccg cagatgatat cattgtcaat gaaattgccc
    18781 cacgtcccca caactcaggg cactactcaa tcgaggcttg cgacttttca cagtttgaca
    18841 cccacatttt gggcgtgcta ggagcgccgc tcccaaccat ccacctccat gctccagccg
    18901 tcatgctcaa tgtcctcgga caacacgtcc agcaggcaat tgactatgtt gcccaaaacc
    18961 ctagcgccca cctccacatg tatggtaaac tagaagcgaa acataaccgc aaaatgggac
    19021 atgtgacggt gtttagtgat ttgcctgatg aggtggagga gttttaggat gtatctaaag
    19081 tatatattta aaataaagga taggaattta gtatcggatt atgaatcttt agttcgaaat
    19141 ttgacaatca atcttcaaaa actagttatt aaagatagaa ctgcattaat aggggataaa
    19201 caacttccaa ttacgatata ttatcgtggg gagaatgaaa agtttcctac gggaatagta
    19261 aaagttgaat cagactcagt tgaaggatta gagttatttg ataaaacgat aaaggaaata
    19321 gttgggaatg aaagtagatt tggaaagttc acatgtatat acgatggaat aagtacatat
    19381 tattcaacag aaatttattc tgaacttcat gaaattgagc gtcgaacaca agagttttta
    19441 agggaagcat tttttctgag cgataaaagt ggggaattga aagaatatca ggcaactatc
    19501 aaaaaaatgg atagtagtga tttctcattc agcaaattaa ttaatgaaat ttttaaagca
    19561 agcgatataa ctgatacatt tattgaccat gttagagcgt gtgttgaatt tggaacagtt
    19621 ccaaatggag atttattacc aaaaagtttt tgggatagtt tagatgactc tttactagat
    19681 gataaagagc aaggtttaga gattaataat ttattaaatg agattcgttc aacaagaaat
    19741 acaatagctc actgtaaaga gtttaaaaag aaggattata ttaattgtat agaacaagtt
    19801 aaaaaattaa agcaagcatt ggataaaata atagcctcta tagagtctac agatatttct
    19861 aaaatgtcac gagaagtgtc acgcataaat aactccttac aagccgataa tgactacatc
    19921 aaacaaaaat attttataga atatgatatt tttgaaaagg atatattagc taggacaggg
    19981 ttattagaaa ttgattggga aaacgtttct gatgacatag agcccttaaa gatacatcgt
    20041 aacttttatg aatcaattag tgaaaaaaca gattctgaag ttatagaaga aatcaaaaat
    20101 ttagaagata taaatttcac tgatgaaaag ggtaatgatt tggatgactt aaccattatt
    20161 gttcctgcgc aagacgaagg atttaaaaga gtctttctag gtcaacgaga atggtatgat
    20221 atacggatag gaaaatcgaa aaggaatagg attaaatata tcgcaggata tgaagttgca
    20281 ccagagagtg gtgtaaagta ctgggctgaa gtggataaaa tagttccttc agataactat
    20341 gttggttact ggaaagtaat ttttaaagga gaggtgaagg aatattcaga atataaaaga
    20401 ttaggaaata cttggccccc tcaaaacagt agatacgtat ctaaaaaggc tttagatgaa
    20461 gctaaatatt tatcggatgt tttttaatta aaggagaaaa aacaaaatga tcgaacgtta
    20521 ttcacgccct gagatggcgg caatttggac agaggaaaat aaataccgtg cttggttgga
    20581 ggttgagatt ctggctgacg aggcatgggc agagttgggt gagattccta aggaggatgt
    20641 ggctaagatt cgtgagaagg cggattttga cattgaccgc attcttgaga ttgagcagga
    20701 gacgcgtcac gatgtggtgg ctttcacgcg tgcggtttct gaaacgcttg gtgaggagcg
    20761 caagtgggtg cactacggtt tgacctcgac tgacgtggtg gacactgcct acggttacct
    20821 ctacaagcaa gctaacgaca ttatccgtcg cgatcttgag aatttcacca atatcgtggc
    20881 agacaaggcg cgtgagcaca aaatgaccat catgatgggt cgtacccacg gtgttcacgc
    20941 cgagccaacg actttcggtc ttaagttggc gacttggtac agcgagatga aacgtaatat
    21001 tgagcgtttt gaacatgctg ctgcaggtgt ggaagctggt aagatttcag gtgccgttgg
    21061 taactttgcc aacatcccac cttttgtgga agaatatgtc tgtgacaaat taggcattcg
    21121 tccgcaagaa atttcaacac aagttcttcc acgtgacctt cacgcagaat actttgcagt
    21181 gcttgcaagt attgcaactt ctatcgaacg tatggcgaca gagattcgtg gtctgcaaaa
    21241 atcagaacaa cgtgaagttg aagagttctt tgccaaaggt cagaaaggta gctctgctat
    21301 gcctcacaaa cgcaacccaa tcggttcaga gaacatgaca gggctagcgc gcgtgattcg
    21361 tggtcacatg gtgacagctt atgagaacgt gtcactttgg catgagcgtg atatttcaca
    21421 ctcatcagct gagcgtatca tcacacctga cacaactatc ttgattgact acatgctcaa
    21481 ccgctttggc aatatcgtta agaacttgac tgtcttcccg gaaaatatga tgcgcaatat
    21541 ggaatcaact tttggtttaa tttatagtca acgtgttatg ctcaaattga ttgaaaaagg
    21601 aatgacacga gaagaagctt atgacttagt tcaacctaag acagcttatt cctgggacaa
    21661 tcaagtggat ttcaaaccac ttttagaaga agacaccaaa gttacctctt gtcttacaca
    21721 agaagaactt gatgaactat ttaatccgat ttattacaca aaacgtgttg atgatatttt
    21781 taagcgttta gggatttaat ataaaaaaaa gaaggtgatt cccttctttt ttatttaaaa
    21841 aacgaatatt ttctttaaaa atattaaaaa tgttagtttt taattagacg ctgtgatata
    21901 ataaagaaaa cagttgttgg agagaaacca tgttagaaca ttttggtgga aaagtaaaag
    21961 tgttaagact tgaaaagagg attagtcgcg aggacttgtg tggggatgag tctgaacttt
    22021 ctgttcgtca attagcacgg atagaactag gtcaatccat accaagttta agtaaggtta
    22081 tttttattgc aaaagcctta aacgttagtg tcggttactt aactgatggt gctgatttag
    22141 aactacctaa gcgttacaaa gaattaaaat accttatctt aaggacacca acttacatgg
    22201 atgatggaaa attacaagta cgagaagagc agtttgatga aatttttgag gattattatg
    22261 ataaattacc agaggaagag aaaataatca ttgattgttt acaggcaact ttagatactt
    22321 tattgagtga gaatactaac tttggcattg acttacttca agaatatttt aatcaaataa
    22381 agactaaggt acgctttagg caaa
//
LOCUS       DATXQK010000022        21670 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.022, whole genome shotgun sequence.
ACCESSION   DATXQK010000022 DATXQK010000000
VERSION     DATXQK010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21670
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.022"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            79..333
                     /locus_tag="VC394_000990"
     CDS             79..333
                     /locus_tag="VC394_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEP5994467.1"
                     /translation="MSYEKEFLKDFEDWVKTQIQVNQLAMATSQEVAQEDGDERAKDA
                     FIRYESKLDAYEFLLGKFDNYKNGKAFHDIPDELFGARHY"
     gene            354..749
                     /locus_tag="VC394_000991"
     CDS             354..749
                     /locus_tag="VC394_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEP5994468.1"
                     /translation="MAKQKNRKKALKLEMKRNGLLKKAGQVFDKAVESVETAVDKTIS
                     AGKNLVEKGSQTVENLTASKERLTLDEFAGQPVVAGIRSDLVETLYAEGIHSAQAFKE
                     WTEKDLLALKGIGPATVKKLVENGASFKK"
     gene            1035..1354
                     /locus_tag="VC394_000992"
                     /pseudo
     CDS             1035..1354
                     /locus_tag="VC394_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351072.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            1351..1914
                     /locus_tag="VC394_000993"
     CDS             1351..1914
                     /locus_tag="VC394_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEP5994469.1"
                     /translation="MNLIIIGAQASGKMTIEQEVARQTGMNLFHNHDSIDFVLRFMPW
                     SQESTALIERMRFAFFETFAKTGQDMIFTIVIDFNDPNDVAMLEKIQAVFQSYDQEVL
                     FVELKTDIEERLKRNRTENRLKHKPLKRNIEWSEQDIQSTMAYAVFNPEEPPKTLTHY
                     QKINNTHLTAAETAQLIIQKMTHIKEN"
     gene            1916..4564
                     /locus_tag="VC394_000994"
     CDS             1916..4564
                     /locus_tag="VC394_000994"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEP5994470.1"
                     /translation="MTELSPKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIPP
                     PNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLREQGI
                     SRYDLGRDKFLDKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVFV
                     DLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALQVATT
                     RPETMFGDVAVAVNPEDPRYKDLIGKNVILPIVNKLIPIVGDEHADPEFGTGVVKITP
                     AHDPNDFEVGQRHNLPQVNVMNDDGTMNELAGDFAGMDRFEARQATVAKLEELGALVN
                     IEKRVHSVGHSERSGAVVEPRLSTQWFVKMDELAKQAMDNQETDNRVDFYPPRFNDTF
                     LQWMENVHDWVISRQLWWGHQIPAWYNAEGEIYVGEEAPEGDGWTQDEDVLDTWFSSA
                     LWPFSTMGWPDTDVEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVLI
                     HGLIRDEEGRKMSKSLGNGIDPMDVIEKYGADSLRWFLSNGSAPGQDVRFSYEKMDAS
                     WNFINKIWNISRYILMNNEGLTLEEAESNVAKVAASEAGNVTDQWILHNLNETIAKVT
                     ENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEAEKVITRSVLLYTLDKIV
                     RLLHPIMPFVTEEIYAQYAQGSIVTVDYPVVRPAFENEAAHKGVERLKDLIRAVRNAR
                     AEVNVAPSKPITILVKTADSELEDFFTSNVNYIKRFTNPEKLEISSAIAAPELAMTSI
                     ITGAEIYLPLADLLNVEEELARLDKELAKWQKELDMVGKKLGNERFVANAKPEVIQKE
                     KDKQADYQAKYDATQERIAEMKKIKS"
     gene            4718..7126
                     /locus_tag="VC394_000995"
     CDS             4718..7126
                     /locus_tag="VC394_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HEP5994471.1"
                     /translation="MRMILAHYDCKKDKKQSLDEHLWHVACSSRQEASIIGQGDVLFL
                     IGLYHDLGKADRTFQDKLLNNPNRHVDHSYAGAKYLCSIIGPHLKNRGVDKNERMTFN
                     EMVGYVISAHHGMYDLCYYFDDAEYYGFNKFKNRINRDLDGYHYHEDIKGYALKLEKK
                     LCDYGYKDLRELIDKAFDNYQQAMSSLNWQDKSEWDYYQSCMVRLYLSLLKNADILDT
                     VNAYGLKISPMDKTERSFLKHSYLAAIEQKYASFGQPNNQLNTIRTEIAERVKERGKR
                     DSKGIYRLDLPTGAGKTNLSMRYAFHQLVHHDKSRFFYITPFLSVLEQNASEIRKVTG
                     DLGVLEHHSNVVKQANEDDDDKDSLLSAYLSDSWDSQVVLTSMVQFFQTLFKTKSANL
                     RRFSSLINSVVILDEVQSLPIEVTTLFNLTMNFLNKVMDTTIVLCTATQPAYDSSEID
                     HRICYGGNLGELAEIVELTIEEKQIFSRTELRKFDDSDQKVHLTDVINLILGEENSVL
                     AIFNTKKTVHNCYTMLKDITDRPVYQLSTNMCAQHRLDLIAKIKTELQNNIPIICIST
                     QLIEAGVDVDFHRVIRSYSGIDSIVQAAGRCNREGKRDKGQVTLVNLTNEEENISRLT
                     EIKAKKEATESILHKIGSPIDISTLNRDFFEYYYANNQGLMDYPLEDNLSIYDYLSLN
                     IYQTANKKFKGKLKQAFKTAGAKMNLINNDMIGILVPYGEAEKKLAYLEELGMSHFLS
                     AEDYQTIKSLLKELQPFTVNVRENDPLFETTKSYLNGQILVLTSEYYDTERGVKYDSA
                     SFYF"
     gene            7259..7987
                     /gene="cas5c"
                     /locus_tag="VC394_000996"
     CDS             7259..7987
                     /gene="cas5c"
                     /locus_tag="VC394_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5"
                     /protein_id="HEP5994472.1"
                     /translation="MYRSRDFYVRVSGQRALFTNPATKGGSERSSYSVPTRQALNGIV
                     DAIYYKPTFTNIVTEVKVINQIQTELQGVRALLHDYSADLSYVSYLSDVVYLIKFHFV
                     WNEDRKDLNSDRLPAKHEAIMERSIRKGGRRDVFLGTRECLGLVDDISQEEYETTVSY
                     YNGVNIDLGIMFHSFAYPKDKKTPLKSYFTKTVMKNGVITFKAQSECDIVNTLSSYAF
                     KAPEEIKSVNDECMEYDAMEKGEN"
     gene            7987..9882
                     /gene="cas8c"
                     /locus_tag="VC394_000997"
     CDS             7987..9882
                     /gene="cas8c"
                     /locus_tag="VC394_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HEP5994473.1"
                     /translation="MDFFTSLLKTYEKAELADLVDHQKRSNEPVLLPIYHTSLKSNGK
                     NIISVKLDKDGQFHKAEFMADKQMIIFPVTADSVARSGSHPAPHPLVDKFAYYSAEMG
                     QSQYDSFHKQLNNWIDYCEEGDVKRFLTIVQQFILKPEFLTLILDSLIGPDYQNNQLK
                     VTFCDATGKEKLIDLSACFLEFSIDQFQGFKNESVSTFKTLHQSYISFVEANREKLGI
                     CNISGREEQLTDKHRGLMGNAKIISVSNKREAYKGRFREREDVFSVGYETSEKIHLML
                     KYLLENKNTSTWLGSSQYLINWFSDDLTNDSRLDIVSPIFDDGLEEDDDDDTPPVITL
                     ATEDNKRIGKSFIKGQKLFANDATYYVAILNKTSNGRIALKYFRQLQASQLLTNLNKW
                     QETYSWESRSKFGKSRLRTPTFHDILNVSYGVDRDRFLELDNDNFKSDQIQKLVASLI
                     DGKPMPQSIVKKLGNNVKERHRYRKHWYQVEQVCLAILHKQNGEEFSPMLDHTNQNRS
                     YLFGRLLAIFELIETLRYGLDGNNNDRITNAERYWTAYTGQPTKLMMLLENKIKPYEE
                     PLKLNRRGSWMKLEKEKEEILELLNPLLETETMEKPLDYRFIFGYYAEKNYYYTKQNT
                     EVTESEE"
     gene            9887..10735
                     /gene="cas7c"
                     /locus_tag="VC394_000998"
     CDS             9887..10735
                     /gene="cas7c"
                     /locus_tag="VC394_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HEP5994474.1"
                     /translation="MLEHKIDFMVTLEVKEANANGDPLNGNMPRTDAKGYGVMSDVSI
                     KRKIRNRLQDMGKSIFVQANERIEDDFRSLERRFSQHFTAKTPDKEIEEKANALWFDV
                     RAFGQVFTYLKKSIGVRGPVSISMAKSLEPIVISSLQITRSTNGMEAKNNSGRSSDTM
                     GTKHFVDYGVYVLKGSINAYFAEKTGFSQEDAEAIKEVLVSLFENDASSARPEGSMRV
                     CEVFWFTHSSKLGNVSSARVFDLLEYHQSIEEKSTYDAYQIHLNQEKLAKYEAKGLTV
                     EILEGL"
     gene            10737..11411
                     /gene="cas4"
                     /locus_tag="VC394_000999"
     CDS             10737..11411
                     /gene="cas4"
                     /locus_tag="VC394_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HEP5994475.1"
                     /translation="MVYAEDDYLMLSGIQHFQFCKRQWALIHIEQQWLDNEATAHGQV
                     LHTKADNPYIKEKRKELLVSRAMPISSAELGLSGIMDVVEFYKDDQGVSLRGKRGKWL
                     PKVVEYKRGKPKKDTRDIVQLVAQTMCLEETLDCDINEGCLYYHSVNQRVIVPMTSAL
                     RQEVKELAAEMQEVYQSQMLPKAAYFKNCQLCSLVDICKPRLSKKTRSVSRYINEAMT
                     SEEMDL"
     gene            11408..12433
                     /gene="cas1c"
                     /locus_tag="VC394_001000"
     CDS             11408..12433
                     /gene="cas1c"
                     /locus_tag="VC394_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated endonuclease Cas1"
                     /protein_id="HEP5994476.1"
                     /translation="MKKLLNTLYLTQEDFYVTKEGDNIVIKQEGKVLKRFPFRIIDGI
                     VCFSYLGVSPALVKLCTENQINLSFHTPQGRFCGRYIGSTNGNVLLRREHYRLSDREE
                     SLEYAKRFILAKISNSRKYLLRFKRDHRQQIDTKLFEAVNDELIWALEMVQAADNKDS
                     LRGIEGQAANQYFRIFNDLVLTDKKTFYFQGRSKRPPLDCVNALLSFGYSLLTFECQS
                     ALEAVGLDSYVGFFHTDRPGCASLALDLVEEFRSYIVDRFVFSLINKGQLQKKHFEVK
                     ENGSILMTENGRAIFIDLWQKRKHTEVEHPFTKEKVKLMLLPYVQAQLLAKAIRGDLE
                     SYPPFMV"
     gene            12444..12737
                     /gene="cas2"
                     /locus_tag="VC394_001001"
     CDS             12444..12737
                     /gene="cas2"
                     /locus_tag="VC394_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002280569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEP5994477.1"
                     /translation="MMVLVTYDVNTETPAGRKRLRHVAKLCVDYGQRVQNSVFECSVT
                     PAEFVDIKHRLTQIIDEKTDSIRFYLLGKNWQRRVETLGRSDSYDPDKGVLLL"
     gene            13273..13983
                     /gene="ccdA2"
                     /locus_tag="VC394_001002"
     CDS             13273..13983
                     /gene="ccdA2"
                     /locus_tag="VC394_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEP5994478.1"
                     /translation="MTLSLVLMVSVFGAGLLSFFSPCIFPVLPVYLGILLDADDSKTI
                     TIFGKKLYWYGIVKTLAFIFGLSTIFVILGYGAGFLGNILYAVWFRYLLGALVIILGI
                     HQMGLITIKSLQFQKSLTFHNNKNRNGLFNAFILGLTFSFGWTPCVGPVLSSVLALVA
                     SGGNGAWQGGVLMIIYTLGLGIPFLLISFASGIVLKQFNKLKPHIFLLKKVGGVLIIV
                     MGILLMTGTLNNLAQLFG"
     gene            13996..14619
                     /locus_tag="VC394_001003"
     CDS             13996..14619
                     /locus_tag="VC394_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEP5994479.1"
                     /translation="MKKGLLVTTGLACLGLLTACSTQDNMVKKEITQDKMSMAVKKKD
                     KMSTSKDKSMMADKSSDKKMTNDGPMAPDFELKGIDGKTYRLSEFKGKKVYLKFWASW
                     CSICLSTLADTEDLAKMSDKDYVVLTVVSPGHQGEKSEADFTKWFQGTDYKDLPVLLD
                     PDGKLLEAYGVRSYPTEVFIGSDGVLAKKHIGYAKKSDIKKTLKGIH"
     gene            14662..15768
                     /gene="msrB"
                     /locus_tag="VC394_001004"
     CDS             14662..15768
                     /gene="msrB"
                     /locus_tag="VC394_001004"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEP5994480.1"
                     /translation="MEHKWKLWLFIVGVVLLIGGASVLIQKGQAKAFSQGKPQLPAKK
                     VKSTKVDPNAPVDQKVIYLAGGCFWGVEEYFSQVDGVLDAVSGYANGRGDTTNYQLIH
                     QTGHAETVEVTYDANRISLKELLLHFFRIIDPTSLNKQGNDRGSQYRTGIYYTDKADL
                     AIIDEVFKEKAKDYKKKIVVEKAPLKHFIKAEDYHQDYLKKNPNGYCHIDINQATYPV
                     IDESKYPKPSATEIKAKLSADEYRVTQKNETEKAFSNRYWDSFDAGIYVDVVTGEPLF
                     SSKDKFESGCGWPSFSRPISPDVVRYKEDKSFNMTRTEVRSRSGNSHLGHVFTDGPKD
                     QGGLRYCINSLSITFIPKADMEAKGYGYLLSSVE"
     gene            15856..16596
                     /locus_tag="VC394_001005"
     CDS             15856..16596
                     /locus_tag="VC394_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEP5994481.1"
                     /translation="MYSLLIVEDEYLVRQGIRSLVDFSQFKIDRVNEAENGQLAWDLF
                     QKEPYDIVLTDINMPKLNGIQLAELIKQESPQTHLVFLTGYDDFNYALSALKLGADDY
                     LLKPFSKADVEDMLGKLQQKLDLSKKTETIQELVEQPQKEVSAIAMAIHERLADSDLT
                     LKSLAQQLGFSPNYLSVLIKKDLGMPFQDYLVQERLKKAKLLLLTSNLKIYEIAEQVG
                     FEDMNYFSQRFKQLVGVTPSQYKKGGQA"
     gene            16593..18326
                     /locus_tag="VC394_001006"
     CDS             16593..18326
                     /locus_tag="VC394_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEP5994482.1"
                     /translation="MKRYPLLVQLISYVFVIVIALITTLGLLYYQTSSRNIRQLIERD
                     TRQSIRQSSQFIDAYIKPLKETTSVLAKNTEIQAFASQIHQENDKQVLQLMKMVLATN
                     SDLQAAVLVTKDGRTVSTNSQLTMKTSSDMMAEPWYKAAIDRQAMPVLTPARQLSLSS
                     KKEWVVSVTQEVVDRAGHNLGVLRLDIAYPTIKASLDQLQLGRQGFAFIVNDKHEFVY
                     HPKKSVYSSSKEMAAMKPYLAIQNGYTKDKTSFVYQKLIPNSQWTLVGVASLDQLHRV
                     QRQIFWSFLGTGLLACLICGFATVLALRRWIRPIQQLQQVILAIQKGDRQLRAQETGS
                     PELTNLAQQFNALLDQIDSLMVAVADKEKAIGQYRLQALASQINPHFLYNTLDTIIWM
                     AEFNDSKRVVEVTKSLAKYFRLALNQGNEYIRLADELDHVSQYLFIQKQRYGDKLSYE
                     VQGLDVYADFVIPKLILQPLVENAIYHGIKEVDRKGMIKVTVSDTAQHLVLTVWDNGK
                     GIEDSSLTNSQSLLARGGVGLKNVDQRLKLHYGEGYHMTIHSQSDQFTEIQLTLPKMH
                     ELMADDTQENE"
     gene            18399..19478
                     /locus_tag="VC394_001007"
     CDS             18399..19478
                     /locus_tag="VC394_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEP5994483.1"
                     /translation="MSLASDYFSRQTPIVEKLMAYGFEKRNNGYFYNERFMEEEFEAQ
                     LRIDEAGNIWDRVIDCDLEEDYLPLQQAAWQGTYTGQVRAAYLELLERLSVACFEVTP
                     FQSMQANRLAKHITKEWSDPMDYPFEKHPDLATYRVEGKWYAMIFSLLADKLDQIPER
                     LVGQTCEVMTVKVNPKAFPQLLQQEGIYPAYHMSKKNWISIILDDKVTDDKLWTLVTQ
                     SRQLVNPNGLSNPNGPDYWVIPANLKYYDIDAEFAANDVILWTQKAGIAVGDYVLIYI
                     TAPVKSIRYVCQVLETNIPNEGYRKNPNIDKLMRLRKRQQYKDGLLSFDLMKKHGVAA
                     VRGPRRLSPQLIAFLKEKEYFKENN"
     gene            19492..20208
                     /locus_tag="VC394_001008"
     CDS             19492..20208
                     /locus_tag="VC394_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HEP5994484.1"
                     /translation="MVEFVADQSFWELFPDAKLGVVLLKDVQNQAESPEDVKQLLADS
                     HELAKAYLTADNFSDNQVVQVYRKAYQHFKTKKGARSSIEALLKRVSNGQSIPSINPL
                     VDIYNAASLRFGLPVGAEDSDSFIGDLRLTITDGGDDFYLIGDADNNPTLPNELCYKD
                     DIGAVCRCLNWRDGERTMVTEHTKNAFLIIEALDQEGQNRLQEALDFIEGSAKMYLHA
                     ITSVHVLDKDNPHVPLFTEN"
     gene            complement(20374..20847)
                     /locus_tag="VC394_001009"
     CDS             complement(20374..20847)
                     /locus_tag="VC394_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEP5994485.1"
                     /translation="MNKKETRHQLIRSLISETTIHTQQELQERLQKNGITITQATLSR
                     DMKELNLVKVTSGNDTHYEALAISQTRWEHRLRFYMEDALVMLKIVQHQIILKTLPGL
                     AQSFGSILDAMQIPEIVATICGDDTCLIVCEDNEQAKACYETLSHYTPPFFFSNK"
     gene            20989..21669
                     /locus_tag="VC394_001010"
     CDS             20989..21669
                     /locus_tag="VC394_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEP5994486.1"
                     /translation="MIRREDYQYLRKLNDFRYFSIEQFDKIVGQMEFRKAKKDHILFF
                     EGDKRDKLFLVTSGYFKVEQSDQSGTFMYTDFIRHGTIFPYGGLFTDDYYHFSVVAMT
                     DVTYFYFPVDLFEDYSLENRLQMKHLYSKMSKLLELHELRVRNLITSSASSRVIQSLA
                     ILLVEMGKDSDTLPFQLTTTDIAQISGTTRETVSHVLRDLKKQELITIKGKYLTYLDK
                     DYFLQYTK"
BASE COUNT         6801 a         3866 c         4705 g         6298 t
ORIGIN      
        1 cagttattaa taacatggca caagaggcct gccttatggt ataatggtaa agatgatttc
       61 agaaagagga agctaagcat gtcttatgaa aaagaatttt taaaagattt tgaagactgg
      121 gttaaaactc agattcaagt caatcagcta gccatggcaa ccagtcaaga agttgcccaa
      181 gaagatggcg atgaaagggc taaagatgcc tttattcgtt atgaaagtaa gttagacgcc
      241 tacgaatttt tactaggtaa atttgacaat tataaaaatg gtaaagcctt tcacgatatt
      301 ccagatgagt tatttggagc aaggcattat taaggacaag gagacacgaa atagtggcaa
      361 aacaaaaaaa tcgtaaaaaa gcattgaagt tagaaatgaa gcgtaatggc ttgttaaaaa
      421 aagcaggtca agtttttgat aaggcagttg aaagtgttga aacagccgtg gataaaacca
      481 tttctgctgg taaaaatctt gtggaaaaag gcagccaaac ggttgaaaac ctgacagcaa
      541 gcaaggaaag acttactctt gatgagtttg cagggcagcc agttgtagct ggtatccgct
      601 ctgaccttgt cgaaacccta tatgcagaag gcatccattc agcgcaagct tttaaggagt
      661 ggacagaaaa agacctcctt gctcttaagg gcattggccc agcaacggtt aaaaaattgg
      721 tcgaaaatgg cgcaagcttt aaaaaatagt catcttctcg ttgcttaatg gctataaatt
      781 tgttagaata ttagcaatca gaggtgcatt gagttggtct gttacagaga gcggtggtgt
      841 tgagaaatcc gcacagatgt caaggctggt agctgaggat aagaaatcag atatgaaaag
      901 tagcagatcc attttatggt ctgaaacagt ggtggtaccg cggaaatcgt aacacgaaat
      961 tcgtccctgt ccagtgtaag ctggataggg attatttggt ttcatttgaa agaagatgat
     1021 ggcttattgt taaaatgttg ttagaagtat acaatagaaa aactttgccc aaagtggaaa
     1081 cagaacggct gctattacgt caacgaaaag gggacaaggc attattccag aagcggcttc
     1141 agctctgatt gaaattggtt ttaccttact tgatttgcat aaaattgagc tgggttgcta
     1201 cgactacaac aagcaaagtc aggcagtggc tcgcaaatta ggctttacct tagaagccaa
     1261 tgtccgtgag cgtaaggacg ttcaaggtcg ccgctgtggc ggcatgcgtt ttgggttact
     1321 gagaagtgag tgggaggagc agaaacaaaa atgaacctta tcatcattgg tgctcaagcg
     1381 tcgggcaaga tgaccattga gcaagaagtt gccaggcaga caggcatgaa tttgtttcat
     1441 aatcacgatt ctattgattt tgttttacgg tttatgcctt ggtctcaaga gagcactgcc
     1501 ttgattgaac gtatgcgttt tgcttttttt gaaacctttg ctaagacagg tcaagacatg
     1561 atttttacta ttgtaattga cttcaatgat ccaaatgatg tagcaatgct tgaaaagatt
     1621 caggctgttt ttcagtctta tgaccaagag gttctctttg tcgaattaaa aacagatata
     1681 gaggaacgtc ttaaacgcaa tcgcacagaa aatcgtctca aacataaacc tcttaaacgc
     1741 aacatcgagt ggtcagaaca agatattcaa tccacgatgg catatgctgt ttttaatcca
     1801 gaagaaccac ccaaaacgct cacgcattac caaaaaatca ataataccca tctcaccgct
     1861 gctgaaacag cgcagcttat tatccaaaaa atgactcata tcaaggagaa ctaagatgac
     1921 agaactatca cctaaataca atcctgccga ggttgaggca ggtcgctatc aaaaatggct
     1981 tgatgcagat gtttttaagc catcaggaga ccaaaaagca aaaccgtatt ctatcgtgat
     2041 tccaccgcca aatgtgactg gtaagttgca cttaggacac gcctgggata cgacgcttca
     2101 agatattatc attcgtcaaa aacgcatgca gggctttgat acgctttggc ttccagggat
     2161 ggaccatgca ggaattgcga cacaggctaa ggtagaggag cgtctgcgcg agcaggggat
     2221 ttctcgttac gaccttggtc gtgacaaatt cctagacaag gtctgggagt ggaaagacga
     2281 atatgccact acgattaaag agcagtgggg caaaatgggg ctttctgtgg actacagccg
     2341 tgagcgcttc acccttgatg agggcttgtc aaaagcagtt cgcaaggttt ttgttgacct
     2401 ctacaaaaaa ggctggattt accgtggtga attcatcatc aactgggatc ctgctgccag
     2461 aactgccctt tctgacatcg aggttatcca caaggatgtt gagggagctt tctatcacat
     2521 gaactatatg ttggaagatg gctcacgcgc ccttcaagtg gcaacaacgc gaccagaaac
     2581 catgtttgga gacgttgccg ttgccgtaaa cccagaagat cctcgttaca aagatttgat
     2641 tggcaaaaat gttattttac caatcgtgaa taaattgatt ccaatcgtgg gtgacgagca
     2701 tgctgaccct gaatttggga ctggagttgt gaaaatcacg cctgcccatg atcctaacga
     2761 tttcgaggtt ggtcaacgcc ataatctacc acaagtgaat gtcatgaacg atgacggtac
     2821 catgaatgag ttagctggcg actttgcagg catggaccgt tttgaagctc gtcaagctac
     2881 cgttgctaaa cttgaagaac ttggtgccct agtaaacatc gaaaaacgtg ttcactcagt
     2941 aggtcactca gaacgttctg gtgcagtcgt tgagcctcgt ctatccaccc aatggtttgt
     3001 aaaaatggat gagcttgcta aacaagctat ggacaaccaa gaaacagata accgagtaga
     3061 tttctaccca ccacgcttta atgatacctt ccttcaatgg atggaaaatg tccatgactg
     3121 ggtaatctcg cgtcagcttt ggtgggggca ccaaattcct gcttggtaca atgcagaagg
     3181 tgagatttac gttggcgaag aagcaccaga aggtgatggc tggactcaag atgaagatgt
     3241 tcttgacact tggttctcat ctgctttatg gccgttctca actatggggt ggcctgacac
     3301 agatgtagaa gatttcaaac gttatttccc aacctcaacc ttagtaacag gttatgacat
     3361 cattttcttc tgggtgtcac gtatgatttt ccaatcgctt gaatttaccg ggcgtcagcc
     3421 attccaaaat gtcctgattc acggtcttat tcgtgacgaa gaaggacgca aaatgtctaa
     3481 atcattgggg aacgggattg atccgatgga tgtcattgag aaatatggtg ccgatagctt
     3541 acgttggttc ctctcaaacg gctctgcccc aggacaagac gtgcgtttct catacgaaaa
     3601 aatggacgcc tcttggaatt tcattaataa aatttggaat atttctcgct atatcctcat
     3661 gaataatgaa gggttgacct tggaagaagc tgaaagtaat gtggctaagg tagctgcctc
     3721 agaagctgga aatgtgactg accaatggat tctccacaat ctcaatgaaa ccatcgctaa
     3781 agtgacagaa aactttgaca agtttgagtt tggtgtggca ggacacattc tttacaactt
     3841 catctgggaa gaatttgcca actggtatgt ggaattgacc aaggaagtgc tatatagcga
     3901 caacgaagct gaaaaagtta tcacgcgctc tgtccttctt tatactttgg acaagattgt
     3961 acgtctcctt cacccaatca tgccatttgt aacagaagaa atttacgctc aatatgccca
     4021 aggttctatt gtgacggtgg actatcctgt ggttcgccca gcttttgaaa atgaagctgc
     4081 gcataaaggt gtggaaaggt tgaaagacct gattcgtgca gttcgtaatg cgcgcgctga
     4141 agttaacgtg gcaccatcaa aaccaatcac cattttggtg aagacagcag atagtgagtt
     4201 ggaagacttc ttcaccagca acgtgaacta catcaaacgt ttcaccaatc ctgaaaaatt
     4261 agaaatcagc tcagccattg ctgcgccaga gctggctatg accagtatca tcacaggtgc
     4321 tgaaatctac ttgccactag cagatttgct taacgtggaa gaagaactcg ctcgcctaga
     4381 taaagaactg gctaaatggc aaaaagaatt ggacatggtc ggcaaaaaac tcggaaacga
     4441 acgctttgta gccaacgcca aaccagaagt catccaaaaa gaaaaagaca aacaagccga
     4501 ctaccaagcc aaatacgacg caacccaaga acggattgca gagatgaaga aaattaaatc
     4561 atagtgttta gaacgcagtt gaaaaactgt gttcttttct tactaataga gatattagat
     4621 tgaagtttaa ttatgagaaa aacgctttct ttatttaaag agttatgtta caatactatt
     4681 acaatcatcg tacaaatagt gttattttga ggggcatatg agaatgattt tagcacacta
     4741 tgactgtaaa aaagataaaa agcaatcttt agatgagcat ttatggcatg tggcctgttc
     4801 tagtcgacag gaagcatcta taattggtca aggagatgtg ctttttttaa ttggtcttta
     4861 ccacgacctg ggcaaagctg atcgaacctt tcaagataaa ttattaaata atccaaatcg
     4921 gcatgttgat cactcttatg caggggcaaa atacttatgt tctattattg ggcctcatct
     4981 aaaaaaccga ggggttgata aaaatgagag aatgacattc aacgaaatgg tggggtatgt
     5041 catctctgct catcatggga tgtatgattt atgctactat tttgacgatg ctgaatatta
     5101 tggctttaat aagtttaaaa atcgtatcaa tagagactta gatggttatc actatcatga
     5161 agatattaaa gggtacgctc taaaattaga aaaaaaatta tgtgattatg gctacaaaga
     5221 tttaagggag cttattgata aagcttttga taattaccaa caagccatgt cttccttaaa
     5281 ctggcaagat aagagtgagt gggattatta tcagtcttgt atggtgagac tttacttgtc
     5341 actcttaaaa aacgctgata ttttggacac agtaaatgcc tatggcctta agataagtcc
     5401 tatggataaa acagagcgat cctttctaaa acactcctat ttagcggcca ttgaacaaaa
     5461 atatgctagc tttggacagc caaacaatca gttgaacact attcggacag aaatcgctga
     5521 gcgtgttaaa gaaagaggta aacgagattc caaggggatt tatcgcttag atttaccgac
     5581 aggagctggc aagactaatc ttagtatgcg ttatgcgttt caccaattag ttcatcacga
     5641 caaatcaagg tttttttaca taacgccctt tctttcggtt cttgaacaaa atgcttccga
     5701 aattagaaaa gttacaggtg accttggcgt cctagaacac cattccaatg tggtgaaaca
     5761 ggctaatgaa gatgatgatg acaaggacag tttattgtca gcttatctta gtgatagctg
     5821 ggacagtcaa gtagtcttga cttctatggt tcaatttttc caaacacttt tcaaaacaaa
     5881 atcagctaat ctgagacgtt tttcaagttt gattaatagt gttgtgattc tagatgaagt
     5941 tcaatccctg cctattgaag tcaccacttt gtttaattta acgatgaatt ttttaaataa
     6001 agttatggat acaaccatcg ttctttgcac agcaacacaa cctgcttatg attcttcaga
     6061 gattgaccat cgtatctgtt atggagggaa cttgggagaa ttagctgaaa tcgttgagtt
     6121 aacgattgaa gaaaaacaga ttttttcaag gacagagctt agaaaatttg atgatagtga
     6181 tcagaaagtt cacttgactg atgttattaa tcttattcta ggtgaggaaa actcagttct
     6241 tgctattttt aatacgaaaa aaacggttca taactgctat actatgctaa aagacattac
     6301 tgatagaccg gtctatcagc tttcgacaaa tatgtgtgcg cagcatagac ttgacttgat
     6361 tgctaagatc aaaacggagt tacaaaataa tatccctatt atttgtatta gcacgcaatt
     6421 aattgaagca ggtgtagatg ttgattttca tcgcgtcatt cgttcctact cagggattga
     6481 ttctattgtt caggctgctg gacggtgtaa ccgagaaggc aaacgagata aagggcaagt
     6541 cactcttgtc aatctgacca atgaagagga aaatatttct aggctgacag aaataaaagc
     6601 taaaaaagaa gccacagaat ctattcttca taagattggg tctccaattg atatctcaac
     6661 tttaaaccgt gacttttttg agtattatta tgccaataat cagggactga tggattatcc
     6721 tttggaagac aacctatcaa tctacgacta tttaagcctt aatatttatc agacggcaaa
     6781 taaaaagttc aaaggtaagt taaaacaagc ttttaaaaca gcaggagcca aaatgaacct
     6841 catcaataat gatatgatag gaattctcgt accttatggc gaagctgaga aaaaattggc
     6901 ttatttagaa gaattgggta tgtctcactt tttatcagca gaagattatc aaacgataaa
     6961 atcattacta aaagagttac aaccttttac ggttaatgtc cgcgagaacg atcctctctt
     7021 tgagacaaca aaatcttatc taaatggtca gattctggtt ttgacgtcgg agtattatga
     7081 cacggaaaga ggagttaaat acgattcagc tagcttttac ttctaactca aaacgaaagc
     7141 agattaacaa aaggttgtta gaggaccttg ttaacctgcc aatcatcatt aataattatt
     7201 atcaatttag actatttaat aaaattagat tacaaaaaaa cagaaggagg aaagtagctt
     7261 gtacagatct agagacttct acgtgagagt aagtggtcag cgagctcttt ttacaaatcc
     7321 agccacaaaa gggggatcgg aacgctcatc ctattcggtt ccgactagac aggcactgaa
     7381 tggtatcgtt gatgccatct attataagcc gacctttact aatatcgtca cagaggttaa
     7441 ggttattaac cagattcaaa ccgaattaca gggtgtcagg gctctgttac atgattatag
     7501 tgcagattta agttatgtat cctatttgag tgatgttgtt tatctgatca agtttcattt
     7561 tgtttggaat gaagatagaa aagatttgaa ctcagataga cttccagcta aacatgaagc
     7621 cattatggag cgttctattc gtaaaggggg acgtcgagat gtgtttttgg gtacaagaga
     7681 atgtttaggg cttgtagatg atatcagcca agaagagtat gagactactg tgtcgtatta
     7741 taatggtgtc aatatcgact tgggaatcat gttccattcc tttgcctatc cgaaggacaa
     7801 aaagacacca ttaaaatcat actttacaaa gactgtgatg aaaaatggag tcattacgtt
     7861 taaagcacag tctgaatgcg atattgttaa cacgctttct agttatgctt ttaaagcacc
     7921 agaggagata aaatcggtta acgatgaatg catggagtat gatgccatgg agaaaggaga
     7981 aaactgatgg atttttttac ttctctcttg aagacttatg aaaaagcaga gctagcagac
     8041 ttggttgatc accaaaaaag aagtaatgag ccggttttac tgccgattta tcatacgagt
     8101 ttaaagtcaa atggcaaaaa tatcatttca gtgaaacttg acaaagatgg ccagtttcac
     8161 aaggcagaat ttatggcaga taagcaaatg attatttttc ctgtaacggc tgattctgtt
     8221 gctaggtcag gtagtcatcc tgcaccgcat cccctagtcg ataaatttgc ttattatagt
     8281 gctgaaatgg ggcagtctca gtatgattct tttcataagc aacttaataa ctggattgat
     8341 tattgtgagg agggtgacgt caagaggttt ttaaccattg ttcagcaatt tattttgaaa
     8401 ccagaatttc tgacactgat tctcgattct ttaattggtc ctgattatca aaataatcaa
     8461 ttaaaagtca cattttgtga tgccactgga aaagaaaaat taattgattt atcagcttgc
     8521 tttttagaat tttcaattga tcagttccag ggctttaaaa atgaatcggt ttcgacattt
     8581 aaaactttac accaatccta tatttctttt gttgaagcca atcgtgaaaa gcttggtatt
     8641 tgtaatatta gtggacgaga ggaacagctt accgataagc atagaggttt gatggggaat
     8701 gctaaaatca tctctgttag taataaaaga gaagcttata aaggacgttt tagagaacgt
     8761 gaagatgttt ttagtgttgg ctatgaaact tccgaaaaga ttcatttaat gctcaagtac
     8821 cttttagaaa ataaaaatac cagtacttgg ttagggtctt ctcaatattt aatcaactgg
     8881 tttagtgatg atttaacaaa tgatagtcgg ttggatatcg tatcaccaat ctttgatgac
     8941 ggacttgaag aagatgatga tgacgatacg cctcctgtta taacattagc aactgaagac
     9001 aataaaagaa ttggtaaatc attcatcaag ggacaaaaat tatttgctaa tgatgccact
     9061 tactacgttg ctattttgaa taaaaccagc aatgggcgga ttgctttaaa atattttcgt
     9121 cagcttcaag cgtcccaatt actcaccaat cttaacaagt ggcaggaaac atacagttgg
     9181 gagtcgcgat ctaagtttgg aaaaagtcgc ttaagaaccc ctacttttca tgacatcctt
     9241 aatgtgtcct acggggttga tagggatcgc ttccttgaat tagataatga taacttcaaa
     9301 agtgatcaaa ttcaaaagtt agtggcaagt ttgattgatg gtaaaccgat gccacagtcc
     9361 attgtcaaaa agttaggtaa caatgttaaa gaacgacatc gttaccgtaa gcactggtat
     9421 caagttgagc aggtctgctt agcaatttta cacaaacaaa atggggagga attttcaccg
     9481 atgctagatc ataccaatca aaatcgttcc tatctttttg gacgattatt agcaattttt
     9541 gaattaatcg agaccttgcg ttatggcttg gatggaaaca ataacgaccg tattaccaat
     9601 gctgaacgtt attggacagc ctatactgga caaccaacaa aattgatgat gttattggaa
     9661 aataaaatta agccttacga agaaccattg aaattaaatc gtcgtggcag ttggatgaaa
     9721 ttagaaaaag aaaaagaaga gattttagaa ctgttaaatc ctctgttaga aacagaaaca
     9781 atggaaaaac ccttagatta ccgctttatt tttgggtatt atgctgagaa aaactattac
     9841 tatacaaaac aaaacactga agtaacagaa agtgaggagt aaaaagatgt tggaacacaa
     9901 aattgatttt atggtaactc ttgaagtgaa agaagcaaat gcaaatggtg atcccttaaa
     9961 tggaaacatg cctcgtacag atgccaaagg atatggtgtg atgagtgatg tctccatcaa
    10021 acgtaagatt cgtaatcgtt tgcaagatat ggggaagtct atttttgtgc aagctaatga
    10081 acgtattgaa gatgactttc gttcactaga aagacgcttt tcacaacatt ttacagctaa
    10141 gacacctgac aaagaaattg aagaaaaagc aaatgcatta tggtttgatg ttcgtgcttt
    10201 tggacaagtt tttacttatc tgaaaaaatc aattggggtg cgtggaccag tttccatcag
    10261 tatggctaag tccttggagc caattgtcat ttccagcctt caaattacgc gtagtaccaa
    10321 tggtatggaa gctaagaata atagtggccg ctcttctgat acgatgggga caaaacattt
    10381 tgtagattat ggtgtgtatg tacttaaagg ttctatcaat gcttattttg ctgaaaagac
    10441 tggtttttct caggaagatg ctgaggctat taaagaagtt ttggttagct tgtttgaaaa
    10501 tgatgcgtcg tctgcacgtc cggaaggctc tatgcgagtt tgtgaagtct tttggtttac
    10561 gcattcaagc aaactgggga atgtttcaag tgcgcgtgtc tttgacttgt tagagtatca
    10621 tcaatcaata gaagaaaaaa gcacttatga cgcttatcag attcatctaa atcaagaaaa
    10681 attggctaaa tatgaagcga aagggttaac ggttgaaatc ctagaaggac tctagtatgg
    10741 tctatgccga agatgattat ttaatgctat caggtattca gcatttccaa ttttgtaaac
    10801 gtcaatgggc gttgatccat attgagcaac aatggcttga taatgaagcg acagcgcatg
    10861 gacaggtttt acatactaaa gcagataacc cttacattaa agaaaaacga aaagagcttt
    10921 tggtctcacg tgctatgccc atttcttctg cagaacttgg actttcagga attatggatg
    10981 ttgtggaatt ttataaagat gatcaaggtg tgtctttgag gggaaaacgt gggaaatggt
    11041 taccaaaagt tgtggaatac aagcgcggaa aacctaaaaa agataccaga gatattgtcc
    11101 agttggtggc tcagaccatg tgtttagaag aaacgctaga ctgcgacatt aacgaaggtt
    11161 gtctttatta ccatagtgtc aatcaaagag tgattgttcc tatgacatca gctttgcgtc
    11221 aagaagtgaa ggaattagcc gcagagatgc aagaggttta tcagagtcaa atgctaccta
    11281 aagcagctta ttttaaaaac tgtcagcttt gttctttagt cgatatttgt aagcccaggt
    11341 tgagtaaaaa aacaaggagt gtgtcgcgtt acatcaatga ggctatgacc agtgaggaga
    11401 tggacctatg aaaaagttgc taaatacctt gtatttgacg caagaagatt tttatgtcac
    11461 taaagagggc gataacattg ttatcaagca agaaggtaag gttctcaaac ggtttccgtt
    11521 tcggattatt gacggtattg tctgtttttc ttatttgggt gtgtcgcctg ctttggtgaa
    11581 gttatgtacg gagaatcaga ttaatttatc gtttcataca ccacaagggc gtttttgtgg
    11641 tcgctatatt ggttcaacca atgggaatgt gttgttgcgt agagaacatt atcgtttatc
    11701 tgatcgtgag gaatctttgg aatacgcaaa gcggtttatt ttggctaaaa tttccaactc
    11761 aaggaaatac ttgctacgct ttaaacgaga tcatcgtcaa cagattgata ccaagctttt
    11821 tgaggctgtt aatgacgaat tgatatgggc tttagagatg gttcaggcag cagataataa
    11881 agactcttta agagggattg aaggccaagc tgctaatcag tattttcgca tatttaatga
    11941 cctggtgttg acggacaaaa aaacgtttta cttccaaggt cggagtaaac gaccaccctt
    12001 agattgtgtt aatgccctct tgtcttttgg ttacagttta ctgacctttg aatgtcaatc
    12061 tgccttggaa gctgtcggat tagacagtta cgttggtttc tttcacacgg atcgtcctgg
    12121 gtgtgctagt ttagcgcttg atttagttga agagttccgc tcatatattg tagatcgttt
    12181 tgtcttttca ttaattaata aaggacaact tcagaaaaaa cactttgagg ttaaagaaaa
    12241 tggtagtatt ttaatgacgg aaaatggcag agctattttt attgatttgt ggcagaagcg
    12301 taagcatact gaggtagaac atccttttac aaaagagaaa gtaaaactta tgttattacc
    12361 ctatgtacaa gcgcagcttt tagctaaggc catacgagga gatttagaaa gctatccacc
    12421 ttttatggtt taggagatgt tatatgatgg ttttagtcac ttatgatgta aatacggaaa
    12481 cacctgctgg tagaaaaaga ttgcgtcatg ttgccaaact ctgtgtggac tatgggcaac
    12541 gtgttcaaaa ttctgttttt gaatgttctg tgacacccgc agaatttgtg gatataaagc
    12601 accgcttaac acaaatcatt gatgagaaaa ctgatagtat tcgcttttat ttattgggga
    12661 aaaattggca gaggcgtgtg gaaacacttg gtcgctcaga cagctatgac ccagataaag
    12721 gtgtcttatt attgtaaaaa tctcttgtgc gaagctagct ttcacagaaa caccttgctt
    12781 gctcgcgcaa aaataactta aaaaagaagc gaaatggaga taaaaaggct taaaacacct
    12841 ctatgtgtgc catccgtttc tctataaact gtgtcatttg gcgcagtctc acccttcatg
    12901 ggtgagtgga ttgaaataaa taattacatg actagccaat acacccacat agtctcaccc
    12961 ttcatgggtg agtggattga aatttcatga gcttctttac tctcaaagta agagtggtct
    13021 cacccttcat gggtgagtgg attgaaattt catgagcttc tttactctca aagtaagagt
    13081 ggtctcaccc ttcatgggtg agtggattga aagatgaacc cgacctctct tttaatgagg
    13141 tcgggttttt acatgccttt agtcaaccat ttatctgata gcgtatcaaa ttcaagtttt
    13201 tgcatgttct agtctatggt ttgcgccact agctttcggt aaactaaggg taacttaaga
    13261 gaggagttat ttatgacact atcacttgta ttaatggtat cggtttttgg ggcaggcttg
    13321 ttatcctttt tctctccgtg tatctttccg gtccttcctg tttatttagg cattttactg
    13381 gatgcagatg attcaaagac gattactata tttggtaaaa aactctattg gtatggcatt
    13441 gtcaaaacct tagcctttat ttttggtcta tctactattt ttgtgatttt aggttacggg
    13501 gctggttttt taggaaatat cctttatgcg gtatggtttc ggtacttact gggagccttg
    13561 gtcattatct tggggattca tcagatgggc ctcattacca ttaagagctt acaatttcaa
    13621 aaatcactga ctttccataa taacaagaat cgcaacggtt tgttcaatgc atttatcctt
    13681 ggtttaacct ttagctttgg ttggacgcct tgtgtgggac ctgttttgag ttctgttcta
    13741 gctttggtgg cttcaggggg aaatggtgcc tggcaaggtg gcgttttgat gattatttat
    13801 actcttggat tgggcattcc tttcctactc atctcttttg cgtcaggcat tgttttgaaa
    13861 cagtttaaca agctcaaacc ccacatattt ttactgaaaa aagtaggagg cgttctgatt
    13921 atagtcatgg gaatcttgct tatgacagga accttaaata acttagcaca actttttgga
    13981 taaaggagaa acacaatgaa aaaaggacta ttagtaacaa ctggtttggc ttgtctcggg
    14041 ctactaactg cttgctcaac ccaagacaat atggttaaaa aggaaataac tcaggacaag
    14101 atgagcatgg cagttaaaaa gaaagataag atgtcaacat caaaggacaa gtccatgatg
    14161 gcagataaat catctgataa gaaaatgacc aatgatggtc ctatggcgcc tgatttcgaa
    14221 ctcaaaggca tcgatggtaa aacctatcgc ttatcagaat tcaaaggtaa aaaagtttat
    14281 ttgaaatttt gggcctcttg gtgttcaatc tgtttatcaa ccttggcaga taccgaagac
    14341 ctggctaaaa tgtcagataa agactatgtg gtcttaaccg tcgtctcgcc aggtcatcaa
    14401 ggagaaaaat cagaagcaga ctttacaaaa tggttccaag gaacagacta taaggactta
    14461 ccagtcttgt tagatccgga tggcaagcta ttggaagctt acggcgtcag atcttaccca
    14521 acagaagttt tcattggaag tgatggtgtt cttgctaaaa aacacattgg ttatgccaaa
    14581 aaatcagaca tcaaaaagac ccttaaaggt atacattagg aaggaccagc tgataggcat
    14641 tagaaaaaag gagttgagtt tatggaacac aaatggaaat tatggctatt tatcgttgga
    14701 gtggtcttgt taataggcgg agcttctgtt ctcattcaga aaggtcaagc caaggctttt
    14761 agtcaaggca agcctcaact accagctaaa aaagtcaaat ccactaaagt agatcccaat
    14821 gcccctgttg atcaaaaagt gatttacctg gctggaggct gtttctgggg cgttgaagaa
    14881 tatttttcac aagtggatgg cgttctagat gcggtgtcag gctatgccaa tggtagaggc
    14941 gataccacca actaccagtt aattcatcag actggtcatg cagaaaccgt tgaagtgacc
    15001 tatgatgcga accgtattag cttgaaagaa ttattactgc attttttccg aattattgac
    15061 ccaaccagtc ttaataagca aggaaatgat cgcggtagtc aataccgaac tgggatttac
    15121 tatactgaca aagcagattt agctattatt gatgaggtct tcaaagaaaa agccaaggat
    15181 tacaagaaaa aaatagtggt tgaaaaagca ccattgaaac actttattaa ggcagaagac
    15241 taccaccaag attacctcaa gaaaaatcca aatggctact gccatattga catcaaccaa
    15301 gcgacttatc ctgtgattga tgaaagcaag tacccaaaac caagtgccac tgagatcaaa
    15361 gcaaaactat ctgcagacga ataccgagtg acgcaaaaaa acgaaactga aaaagccttt
    15421 tctaatcgct actgggattc ttttgatgca ggaatttacg tagacgttgt gacaggagaa
    15481 ccgcttttct cttcaaaaga caaatttgaa tcaggttgtg gttggccaag ttttagccgc
    15541 cccatcagcc cagacgttgt ccgctacaag gaagataaga gtttcaatat gacgagaaca
    15601 gaagtgcgca gccgttcagg gaattctcat ctggggcatg tctttactga cggacctaaa
    15661 gaccaaggcg gccttcgtta ctgcatcaat agcttgtcta ttacctttat tccaaaagct
    15721 gatatggaag ctaagggcta tggttattta ttatcatctg tagaataggg ttaggcctaa
    15781 aaatctgata taatagaaga aagactagta tgagagggcc atgcaaggcc ctttataaag
    15841 ataaggagac accgtgtgta ctcattgtta attgtagaag acgaatacct tgtgcgccag
    15901 ggtattcgtt ctttggttga ttttagccag ttcaagattg atagggtcaa cgaagcagaa
    15961 aatggccagt tggcttggga cttgtttcag aaagagcctt atgatattgt tttgacggat
    16021 atcaatatgc ccaaattaaa tgggattcaa ctagcagaac tcattaaaca ggaatccccc
    16081 caaactcatc tggtattttt gacgggctac gatgatttta actatgcctt atctgctttg
    16141 aagttagggg cagatgatta cttgctcaaa cccttttcca aggccgatgt ggaagatatg
    16201 ttaggaaagc tccagcaaaa attagacctt tccaagaaaa cagaaaccat ccaggaattg
    16261 gttgagcagc ctcaaaaaga agtatcagca atagcaatgg ctattcatga gcgattggca
    16321 gattctgatt tgaccctaaa aagcttggct cagcagcttg gttttagccc taattacctt
    16381 agcgtcttga ttaaaaaaga tttagggatg ccctttcaag attatctggt acaagaacgg
    16441 ttgaaaaaag ccaagcttct cttgttaacc agtaatctta aaatctatga aattgcagag
    16501 caggtcggtt ttgaggacat gaattatttt tctcagcgct ttaagcaact ggtaggcgtt
    16561 accccaagtc agtataaaaa aggaggccag gcatgaagcg ttatccccta ctggtccagt
    16621 tgatttcgta tgtttttgtg atcgtaattg ccctcattac cacgcttggt ttgctttatt
    16681 accagacgag ttctcgtaat atcaggcaac taattgaacg tgatacccgg cagagcattc
    16741 ggcaaagttc ccaatttatt gacgcttaca tcaagcctct taaagaaaca acttcggtgc
    16801 tggcgaaaaa tacggaaatt caagcctttg ctagtcaaat tcatcaagaa aatgacaaac
    16861 aggttcttca gctcatgaag atggttcttg cgaccaattc cgatttacaa gcagctgttc
    16921 tggtgactaa ggatggtcga acggtgtcta ccaattctca gttgaccatg aaaacctcta
    16981 gtgacatgat ggcagaaccc tggtataaag cagccataga ccgtcaggcc atgccagtct
    17041 tgaccccagc tcggcaatta tccctttctt ctaaaaaaga atgggtagtt tctgtgaccc
    17101 aagaggtagt agatagggct gggcataatt taggtgtgct aagacttgat attgcatacc
    17161 cgaccattaa agcgtcttta gatcagcttc agctaggccg ccaaggcttt gcctttattg
    17221 tgaatgataa gcatgaattt gtttaccatc ccaaaaagag tgtttacagt tcttctaagg
    17281 agatggctgc gatgaagcct tatttagcga ttcagaatgg ttacactaag gacaagacat
    17341 cttttgttta ccaaaaactc attcctaaca gtcaatggac tttagtggga gtggcgtcac
    17401 tggatcagtt gcaccgggtg cagcgccaaa ttttttggtc ctttcttgga acagggcttc
    17461 tagcctgtct gatttgtggc tttgcaactg tcttagcctt acgcagatgg attcgtccca
    17521 ttcaacaatt gcagcaagtt attcttgcta ttcaaaaagg agatcgtcag ttacgtgccc
    17581 aagaaacggg ttctccagaa ttgacaaacc ttgcccaaca gtttaatgcg ctcttagatc
    17641 aaattgatag cttgatggtt gccgttgcgg ataaggaaaa ggcgattggg cagtataggt
    17701 tacaagcctt ggctagtcag attaacccgc attttctcta taacaccttg gacactatta
    17761 tttggatggc agaatttaat gacagcaagc gcgtggtaga agtgaccaag tctctagcta
    17821 agtattttcg tttggccctt aatcagggga acgaatacat tcgtttggca gatgaactgg
    17881 atcacgttag ccaatacctc tttattcaaa aacagcgcta tggagacaag ctaagttatg
    17941 aagtgcaagg cttagatgtc tacgcagact ttgttattcc taagcttatc ttacagccct
    18001 tagtagaaaa tgctatctac catggcatca aagaagtcga tcgcaaaggc atgatcaagg
    18061 ttacggtatc tgatacagct cagcatctgg tgttgactgt ttgggataat ggtaaaggca
    18121 ttgaagactc ttcactgacc aatagtcaga gcttgttggc taggggaggt gtgggcctta
    18181 aaaatgttga ccagcggtta aaacttcact atggtgaagg ctaccacatg accattcata
    18241 gccagtcaga ccagttcact gaaatacaat taacccttcc taaaatgcat gaattaatgg
    18301 cagacgacac acaggaaaat gagtaaggtt acaggaagtg catgtcagcc atttccctaa
    18361 gttagggtat tggagataag aaccttagaa aggatatcat gagtttagca tctgactatt
    18421 ttagccgaca gacccccata gttgaaaagc taatggctta cggttttgaa aaacgaaata
    18481 atggttactt ctataacgaa cggtttatgg aagaagaatt tgaggcgcag cttaggattg
    18541 atgaggctgg caatatctgg gatcgggtta ttgattgtga cttggaggag gattatttgc
    18601 ctttacaaca agcagcctgg caaggaacct atactgggca ggttagagca gcctatttgg
    18661 aactcttgga gcgcttatca gtagcctgtt ttgaagtcac tccttttcag tcaatgcaag
    18721 ctaatagact tgctaagcat atcaccaaag aatggtctga cccaatggac tatccttttg
    18781 aaaagcaccc agaccttgca acctatcggg tagagggcaa atggtatgct atgatttttt
    18841 ctctcttggc tgataaatta gaccagatac cagagagatt agtaggacag acatgtgaag
    18901 tgatgactgt caaagttaat cccaaggctt tccctcagtt actgcagcaa gaaggcattt
    18961 acccagcgta tcacatgtca aagaaaaact ggattagtat tattttggat gataaggtaa
    19021 cagatgataa gctttggacg ttagtaactc aaagtcgtca actagtcaac ccaaacggtc
    19081 tttctaatcc taatggtcct gattattggg ttattccagc taatttgaaa tactatgata
    19141 ttgatgcaga atttgcagcc aatgatgtga ttttatggac ccaaaaagct ggtattgccg
    19201 tgggagacta tgtgctgatt tatataacag ccccagtcaa atccattcgc tatgtctgcc
    19261 aagttctaga aaccaatata cctaacgaag gctatcgtaa aaatcctaac attgacaaac
    19321 tcatgcgtct ccgaaaacgc caacagtaca aggatggtct cttgtctttt gaccttatga
    19381 aaaaacatgg cgtagctgca gtgagagggc caagacgatt aagccctcaa ttaattgcat
    19441 ttttaaagga aaaagagtat tttaaagaga acaactaaag gagaataaga gatggttgaa
    19501 tttgtagcag atcaaagttt ttgggaactt tttccagatg ccaaactagg tgtcgtctta
    19561 ttaaaggatg ttcagaatca agcagagtca ccagaagatg tcaaacagct cttagcagac
    19621 agccatgagt tagcaaaagc ttatttaaca gcagacaatt ttagtgataa tcaggtggtt
    19681 caagtctacc gcaaggccta tcaacacttt aaaaccaaaa aaggagctcg ctctagcatt
    19741 gaagcccttc tcaagcgggt gtcaaatggt caaagcattc cgtcgatcaa tccgctagtg
    19801 gatatttata atgcagctag tctacgtttt ggtttacctg ttggagccga agatagtgac
    19861 agttttatcg gagatttgcg tctgacgata acagacggtg gtgacgactt ttacttgatt
    19921 ggggatgctg acaataaccc aaccttgcca aatgagctgt gttataaaga tgatattgga
    19981 gcggtttgtc gttgccttaa ctggcgagat ggcgaacgca ccatggtgac agaacacacc
    20041 aaaaatgcat ttttgattat cgaagctctt gatcaagaag gtcaaaatag attgcaagaa
    20101 gctcttgact tcattgaagg cagcgcaaaa atgtaccttc atgcaatcac ctctgtgcat
    20161 gtgttggata aagacaatcc acacgtgcca ctttttacgg aaaactagat tagtaggatg
    20221 aactgtgtaa aatagaaggt taacaaaaaa gcgagtaaac gaatgtcctg tcatttaggt
    20281 ttttgtttac tcgctttttg atgagatttt actgctgtcc tagcaaaagg tgaggatgag
    20341 gtcgtatatc acatagaggt tttctgttaa gccttatttg ttactaaaga agaatggtgg
    20401 tgtgtaatga ctaagggtct cgtaacaagc cttggcttgc tcattgtctt cacagacaat
    20461 caagcaagta tcatcaccac agatcgttgc tacaatttct ggaatttgca tggcatctaa
    20521 aatggagcca aaggattggg ctagtccagg taacgttttt aaaataattt gatgttgaac
    20581 tatctttaac atgaccaaag cgtcttccat gtaaaaacga agacgatgtt cccagcgggt
    20641 ttgtgaaatg gctaacgctt catagtgtgt gtcgtttccg gaagttactt tgaccaggtt
    20701 caattccttc atgtcacgtg atagggtagc ctgagtgatc gtgatgccat ttttttgcaa
    20761 ccgttcttgt aattcttgct gtgtatgtat agttgtctca gagatgaggg aacgtatgag
    20821 ttggtgacgc gtctctttct tattcattgt tataatcttc ctttaacgta attgataaca
    20881 atccgagtat agcaaaaaga ggagaaggtg taaagttttt gatgaaccgc ttacaaaatg
    20941 gcttttttat ggtaaaataa agatgatgtt tagaaaaagg agatttgtgt gattcgaaga
    21001 gaagattatc aataccttag aaaactaaac gattttcgct acttttctat tgaacaattt
    21061 gacaaaattg tagggcagat ggaattccga aaagctaaaa aggaccatat cctttttttt
    21121 gagggagata aaagagataa gctttttcta gtgacctctg gttatttcaa ggtggagcag
    21181 tccgatcaat caggaacctt catgtatacg gatttcatca gacacggtac catttttcct
    21241 tacggtggcc tctttacaga tgattattac catttttcag tggtagccat gacagacgtg
    21301 acttattttt actttccagt cgatttattt gaagattatt cgcttgaaaa tcgtctacaa
    21361 atgaaacact tatatagtaa gatgtctaaa ctgctcgagt tacatgagtt gagggttcga
    21421 aatttgatta catcaagtgc tagttctcgt gtgattcagt ctctggcgat tttgctggtt
    21481 gaaatgggta aagacagtga taccttacct tttcaattaa caacaacaga tattgctcaa
    21541 ataagtggga cgacaagaga aactgttagc catgtgctta gggatttgaa gaagcaagaa
    21601 ttgattacca taaaaggtaa gtatttgact tatctagata aagactattt tttacaatac
    21661 acaaagtgaa
//
LOCUS       DATXQK010000023        21247 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.023, whole genome shotgun sequence.
ACCESSION   DATXQK010000023 DATXQK010000000
VERSION     DATXQK010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21247
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.023"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..272)
                     /gene="cadX"
                     /locus_tag="VC394_001011"
     CDS             complement(<1..272)
                     /gene="cadX"
                     /locus_tag="VC394_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cd(II)/Zn(II)-sensing metalloregulatory
                     transcriptional regulator CadX"
                     /protein_id="HEP5994487.1"
                     /translation="MKKDSICQVGVINQQNVTTATNYLEKEKVQKSLRILSKFTDNKQ
                     INIIFYLLAVEELCVCDIACLLNLSMASASHHLRKLANQNILDTRR"
     gene            complement(284..898)
                     /locus_tag="VC394_001012"
     CDS             complement(284..898)
                     /locus_tag="VC394_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEP5994488.1"
                     /translation="MIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIYLGQF
                     LGSVSLILLSLLFAFVLDYIPSKEILGLLGLIPIFLGLKVLLLGDSDGEAIAKEGLSK
                     DNKNLIFLVAMITFASCGADNIGVFVPYFTTLNLANLIVALLTFLVMIYLLVFSAQKL
                     AQVPSVGETLEKYSRWFIAVVYLGLGMYILIENNSFDMLWAVLG"
     gene            complement(1191..1340)
                     /gene="rpmG"
                     /locus_tag="VC394_001013"
     CDS             complement(1191..1340)
                     /gene="rpmG"
                     /locus_tag="VC394_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEP5994489.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVT
                     FTEVK"
     gene            complement(1356..1538)
                     /gene="rpmF"
                     /locus_tag="VC394_001014"
     CDS             complement(1356..1538)
                     /gene="rpmF"
                     /locus_tag="VC394_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEP5994490.1"
                     /translation="MAVPARHTSKAKKNKRRTHYKLTAPSVQFDETTGDYSRSHRVSL
                     KGYYKGRKIAKANEAK"
     gene            1758..3038
                     /locus_tag="VC394_001015"
     CDS             1758..3038
                     /locus_tag="VC394_001015"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEP5994491.1"
                     /translation="MKLQKPKGTQDILPEDAAKWQYVESVARDTFSQYNYGEIRTPMF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PVKLYYIGSMFRYERPQAGRLREFHQIGVECFGAANPATDVETIAMAYHLFEKLGIKD
                     VTLHLNSLGSPESRSAYRQTLIDYLTPMRDQLSKDSQRRLDENPLRVLDSKEKEDKLA
                     VEKAPSILDYLDEESQAHFEAVKDMLEALDIPYVIDTNMVRGLDYYNHTIFEFITSVE
                     GSDLTICAGGRYDSLVGYFGGPETPGFGFGLGLERLLMIIEKQGITLPIETEMDVYLA
                     VLGDSANSKALELVQAIRRQGFTAERDYLGRKIKAQFKSADTFKAKLVMTLGESEVEA
                     GKAVIKNNRSRQEVEVSFEDMMTNFANISEQLLS"
     gene            3376..5124
                     /gene="aspS"
                     /locus_tag="VC394_001016"
     CDS             3376..5124
                     /gene="aspS"
                     /locus_tag="VC394_001016"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEP5994492.1"
                     /translation="MKRSMYAGRVREEHIGTTITLKGWVSRRRDLGGLIFIDLRDREG
                     VMQLVINPEEVSSDVMATAERLRSEYVIEVEGFVEARQQANDKLATGMVELKVSALTI
                     LNTAKTTPFEIKDDVEVSDDTRLRYRYLDLRRPEMLENFKLRAKVTHSIRNYLDDLEF
                     IDVETPMLTKSTPEGARDYLVPSRVSQGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLSEQEIQDIVEGMIAKVMKETKEIDVTLPFPRMSY
                     DVAMNSYGSDKPDTRFEMLLQDLTVTVNGIDFKVFSEAPAVKAIVVKGNADRYSRKDI
                     DKLTEFAKQFGAKGLAWVKVTDGQLAGPVAKFLIAIETELSSQLKLAENDLVLFVADT
                     LEVANNTLGALRNRIAKDLDMIDQSQFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPT
                     PESAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKEMQERMFKALGFTADEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGKGNIREVIAFPKNNKASDPMTQAPSLV
                     SENQLEELSLQIESHD"
     gene            5117..6058
                     /locus_tag="VC394_001017"
     CDS             5117..6058
                     /locus_tag="VC394_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEP5994493.1"
                     /translation="MIKKTTYKKKVKYVISRGAKKVGLLHALRSISREKYAEKISASL
                     LYGILSSIAVNFFFQPGHVYSSGATGLAQVFSALSHRLLGYDFPIAFAFYLINIPLLI
                     LAWYKIGHQFTIFTFITVSMSSFFIQIMPQVTLTTDPLINAIFGGLVMGMGIGTGLKS
                     RISSGGTDIVSLTLRKRTGKDVGSLSLMVNGAILAFAGILFGWQYALYSMVSIFVSSR
                     VTDAIFTKQKKMQATIVTSHPERVIHMIHKRLHRGVTSINDAEGTYKHEQKAVLITIL
                     TCEEYPEFKWLMLKTDPQAFVSVAENVRIIGRFVEDD"
     gene            6078..6956
                     /locus_tag="VC394_001018"
     CDS             6078..6956
                     /locus_tag="VC394_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEP5994494.1"
                     /translation="MSIDQRLQLKKFCFIILGAAIYAFAFVYFYMANRIAANGLAGLT
                     LVGKALFEIDPSLIGYLINLPLVLLGARCFGKRAMVYTVTGVLSLYFFVWLFQRFPLV
                     VDLDHDNLVVSLMAGIIGGLGGGFVFRNGGTIGGADIIAKLLEDKLGLQLNQALLGID
                     LFVMIVSLTYISLPQMMYALIASFMYGQIVRLVQQGGYSARGIFIVSEQAEKIARFIM
                     EELGRGVTYLTGEGAYSGRRKKVIYVALGPRDIRELKAFLTQVDPNAFITYFDVNEVN
                     SPEFLTIKSKYHKKRK"
     gene            6999..7871
                     /locus_tag="VC394_001019"
     CDS             6999..7871
                     /locus_tag="VC394_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEP5994495.1"
                     /translation="MAHHDKLTKLLKLFLIALGVAIYTFGFVNFNMANALAEGGVAGI
                     TLILHAHFGINPAYSSLLFNLPLFILGAKIFGKRSLALTIYGTVLMSAFIWMWQKVPI
                     ELGLENDMMLVAVVAGLFSGIGSGIVFRYGATTGGTDIIGRIAEEKFGAKLGQTLLLV
                     DALVLTASLTYVDLKHMLYTLVASFVFSQMISVVQNGGYTIRGMIIITKHSEAAAQAI
                     LTEINRGVTYLKGQGAYSGNDYNIMYVTLNPTEVREVKRILAGLDPDAFISIIDVDEV
                     ISSDFKIRRRNYDK"
     gene            complement(7898..8206)
                     /locus_tag="VC394_001020"
     CDS             complement(7898..8206)
                     /locus_tag="VC394_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEP5994496.1"
                     /translation="MPSEKEILDALSKVYSEQVIQADDYFRQAIFELASQLEKEGMSS
                     LLATKIDSLINQYILTHQFDAPKSIFDLSRLVKTKASHYKGTAISAIMLGSFLSGGPK
                     "
     gene            complement(8294..9985)
                     /locus_tag="VC394_001021"
     CDS             complement(8294..9985)
                     /locus_tag="VC394_001021"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEP5994497.1"
                     /translation="MDTKTLIASEIAKVVPELEQDAIFNLLETPKNSDMGDLAFPAFS
                     LAKVLRKAPQMIASELAEQIDESQFEKVVAVGPYINFFLDKTKISSQVLEQVITAGSD
                     YAQQDEGQGRNVAIDMSSPNIAKPFSIGHLRSTVIGDSLAHIFAKMGYKPVKINHLGD
                     WGKQFGMLIVAYKKWGDEAAVQAHPIDELLKLYVRINAEAETDPTVDEEAREWFRKLE
                     DGDKEATELWQWFRDESLLEFNRLYDQLHVTFDSYNGEAFYNDKMDEVLDLLEAKNLL
                     VESKGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKRTYDFAKSVYVVGNE
                     QAAHFKQLKAVLKEMGYDWSDDMTHVAFGLVTKGGAKLSTRKGNVILLEPTVAEAINR
                     AASQIEAKNPNLADKEAVAHSVGVGAIKFYDLKTDRMNGYDFDLEAMVSFEGETGPYV
                     QYAHARIQSILRKADFTPSATTTYSLADAESWEIIKLIQDFPRIIKRTSDNFEPSIMA
                     KFAINLAQSFNKYYAHTRILDDNSERDNRLALCYATATVLKEALRLLGVDAPNEM"
     gene            10276..10713
                     /gene="argR"
                     /locus_tag="VC394_001022"
     CDS             10276..10713
                     /gene="argR"
                     /locus_tag="VC394_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEP5994498.1"
                     /translation="MNKMERQQQIKRIIQAEHIGTQEDIKNHLQKEGIVVTQATLSRD
                     LREIGLLKLRDEQGKLYYSLSEPVATPFSPEVRFYVLKVDRAGFMLVLHTNLGEADVL
                     ANLIDNDAIEDILGTIAGADTLLVICRDEEIAKRFEKDLAAGL"
     gene            10710..11062
                     /locus_tag="VC394_001023"
                     /pseudo
     CDS             10710..11062
                     /locus_tag="VC394_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014623648.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RicAFT regulatory complex protein RicA family
                     protein"
     gene            11049..13604
                     /gene="mutS"
                     /locus_tag="VC394_001024"
     CDS             11049..13604
                     /gene="mutS"
                     /locus_tag="VC394_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEP5994499.1"
                     /translation="MAKTNISPGMQQYLDIKKDYPDAFLLFRMGDFYELFYEDAVKAA
                     QLLEIGLTSRNKNAENPIPMAGVPHHSAQQYIDVLIELGYKVAVAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSAKPDSANNFLVAVDFDGCRYGLAYMDVSTGEFCVTDLADFTS
                     VRSEIQNLKAKEVLLGFDLSEEEQTILVKQMNLLLSYEETVYEDKSLIDGQLTTVELT
                     AAGKLLQYVHKTQMRELSHLQALVHYEIKDYLQMSYATKSSLDLVENARTNKKHGSLY
                     WLLDETKTAMGMRLLRSWIDRPLVSKEAILERQEIIQVFLNAFIERTDLSNSLKGVYD
                     IERLSSRVSFGKANPKDLLQLGHTLAQVPYIKAILESFDSPCVDKLVNDIDSLPELEY
                     LIRTAIDPDAPATISEGSIIRTGFDERLDHYRKVMREGTGWIADIEAKERQASGINNL
                     KIDYNKKDGYYFHVTNSNLSLVPEHFFRKATLKNSERYGTAELAKIEGQMLEAREESS
                     SLEYDIFMCIRAQVETYINRLQKLAKILATVDVLQSLAVVAETNHYIRPQFNDNHVIT
                     IQEGRHAVVEKVMGVQEYIPNSISFDQQTSIQLITGPNMSGKSTYMRQLALTVIMAQM
                     GSFVAADHVDLPLFDAIFTRIGAADDLISGQSTFMVEMMEANQAIKRASDNSLILFDE
                     LGRGTATYDGMALAQAIIEYIHDRVGAKTIFATHYHELTDLSTKLTSLVNVHVATLEK
                     DGDVTFLHKIAEGPADKSYGIHVAKIAGLPKSLLKRADEVLTRLETQSRSTEIISVPS
                     QVESSSAVRQGQLSLFGDEEKAHEIRQALEAIDVMNMTPLQAMTTLYELKKLL"
     gene            complement(14001..14654)
                     /locus_tag="VC394_001025"
     CDS             complement(14001..14654)
                     /locus_tag="VC394_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994500.1"
                     /translation="MWSSIFNFKENIVKKKLVMMLTLLISICAISVRANESGDLFTTI
                     GQSHLPGVTVAPFKTGDTTINVNLDPYGYVYGFVTSSNGNKIKKTENKTYEIPCSILE
                     KQDNPNCEGRKWTVSGNRADGGGNYTVQLEKPLAEGDKVTLSFADDGNLYFGQLVFED
                     GKTHNERLQKERDAEEQQYAEVLFKRSIEEESKKTWHQRLSDSIQDQWWNFKDWLRG"
     gene            complement(14819..15202)
                     /locus_tag="VC394_001026"
     CDS             complement(14819..15202)
                     /locus_tag="VC394_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HEP5994501.1"
                     /translation="MSKKNAIRKLKEFHRWQRIANSLDLTYTELYQFDIEYHPTRRKH
                     LEISRECALEELDAIRYAINQLSKVEYRQILIECYLISEEKTQQDIMEELNRSQSWYY
                     ESKKRALLEFVEFYRDGALKNNVRL"
     gene            complement(15177..15539)
                     /locus_tag="VC394_001027"
     CDS             complement(15177..15539)
                     /locus_tag="VC394_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEP5994502.1"
                     /translation="MSAKEQLKELKPLFALITLFEEQRDKDIELINAFHNPEAIRHIE
                     KGTAKQLLYLAKERDKRLAMIATLQDERQIAVIKARYVDDLSWDEILDKLGYSRNIVF
                     KLHREALEVLDEQEERYS"
     gene            complement(15943..16431)
                     /locus_tag="VC394_001028"
     CDS             complement(15943..16431)
                     /locus_tag="VC394_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994503.1"
                     /translation="MLELSIENIIKPMKTQGKTRVTGSIGDQAIRIDLDGLVIHYNGQ
                     GLLLETIPGTYGGKRYFFVCPDCERRCRKLFKASHAFACGSCQKVHQATLNRSKTDCC
                     YYWQLAFKECLKVNPEARHIHGYYSRDDFPKRPKYMRLTKYLYHWRRFHYYMDKGDRY
                     WL"
     gene            complement(16505..17014)
                     /locus_tag="VC394_001029"
     CDS             complement(16505..17014)
                     /locus_tag="VC394_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994504.1"
                     /translation="MRTFSDTPKTFTFHYTFKDFDTAQVACHAILGYMTGTYEQPVID
                     ATYHNDDQGGHANQLVLKYVEDRKLNKVFKRICDSFKDYYNQPEDMTDEELDHQRVVS
                     LSKSTQGKVNNRDTLIAFISDHNQLAEHLSMNYKEMTPEDLGAILESISQAFNHLYDM
                     VVEGQLLVK"
     gene            complement(17016..17189)
                     /locus_tag="VC394_001030"
     CDS             complement(17016..17189)
                     /locus_tag="VC394_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994505.1"
                     /translation="MKLKLHTRGGNTITIQGDRTLYNELVKYLLSGEQPNWVASPSAI
                     INLSDIIAITKEK"
     gene            complement(17195..17368)
                     /locus_tag="VC394_001031"
     CDS             complement(17195..17368)
                     /locus_tag="VC394_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2758 domain-containing protein"
                     /protein_id="HEP5994506.1"
                     /translation="MKIKLFYQKHNESLDDFEHRVNLFTLSVSVIDIKFQEATYGNYE
                     DMDSRTGLLVLYR"
     gene            complement(17365..17619)
                     /locus_tag="VC394_001032"
     CDS             complement(17365..17619)
                     /locus_tag="VC394_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994507.1"
                     /translation="MNPNYTNHGVKKMRYNYDDLPNDQLYRLELPNGQCGYDFITKIN
                     FNIKSRPGDCDFTRPELTRRGYQWVFDCGFAIPVEKGAKT"
     gene            complement(17913..19307)
                     /locus_tag="VC394_001033"
     CDS             complement(17913..19307)
                     /locus_tag="VC394_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence-associated protein E"
                     /protein_id="HEP5994508.1"
                     /translation="MKDYQNKLSQATQPAFAPAFRTRKGRGDKEYVISSPYNVGKVFE
                     FYENIYTGIKYNEFEKTIEITKAVPWSKEKGLWTNEQTSLCIAFIDEKYRFTPRKEHI
                     EVAITALAKKNTYHPIKQRIESQKWDGKARGERYFIDLLGCADNSYNREIAKVWLTGL
                     MARIYLRKVKFEVVPILIDKRQGTGKSTVTKRLLPSYHTDSEIKFGKNDSDYQKIQAN
                     AIIELGELKGMSKAEIETVKSFISSDSDTYREPYERKATPHPRHCVFIGTANKKSFLK
                     DSGTERRFFPIECGINDVKKHPMEVEEDYFLQVLAEAKVWFNNYEPLTPSKELMNQLA
                     DIQEDYKVEDVDKEIIDQLLNEFQIVEGWDSLSQYEQRQYILKQLGEPLDNAQSYSDY
                     PSAQTDCLLQVTSPNHIAYLGFNQKPTQGGKALISQKIRDYLDNDDGWKKGENPARKR
                     LFKGGTPVPYYERV"
     gene            complement(19319..20185)
                     /locus_tag="VC394_001034"
     CDS             complement(19319..20185)
                     /locus_tag="VC394_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase alpha helix C-terminal domain-containing
                     protein"
                     /protein_id="HEP5994509.1"
                     /translation="MAIYEARGFSSYLYPYKGPLEPFDYVAQFRPLKPPEDIDIEEYK
                     RTQAPYCLSGKVTAEKNGSYKRNNASLVYRDLIFLDYDEIETGVNLPKIVSQTLWEYS
                     YIIYPTIKHTPEKPRYRLVMKPSDVMTEATYKQVVKEIADKIGLPFDLASLTWSQLQG
                     LPVTTGDPEDYQRYVNHGLDYPVPKNGSTPNRQVVTTYTPRPRSQRSITMRVIDTLFN
                     GFGDEGGRNVALTKFVGLLFNKWVDCDLETAYELVQIANSVTTKPLPIDEIDTTFRSI
                     LDKELRKRGIKP"
     gene            complement(20186..20458)
                     /locus_tag="VC394_001035"
     CDS             complement(20186..20458)
                     /locus_tag="VC394_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994510.1"
                     /translation="MNVFSQDYEAKLLEQNLTAFNHFLEAYQKPKPRVLGLITAEQVK
                     EELNIKGKTLKRWENAGLRRYQPPLEDTRKHYYKVSDILIFLGVNV"
     gene            complement(20461..20790)
                     /locus_tag="VC394_001036"
     CDS             complement(20461..20790)
                     /locus_tag="VC394_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994511.1"
                     /translation="MTLPENYRRVLNLIKVGADNPITGAEISLILKLEERSVQSIISS
                     LITRYNVPIIGIRHGFNRGYFIPANKEELLDGAKAFYNQVQKEQERLSVLLNADLTSY
                     KELLKGG"
     gene            complement(20802..20993)
                     /locus_tag="VC394_001037"
     CDS             complement(20802..20993)
                     /locus_tag="VC394_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994512.1"
                     /translation="MQELNLTPTQTLILFFVLGLLGLLLSRSKPLIEIDLPEDTHAPK
                     PRQNANYGAYIQAQNHYYN"
     gene            complement(20993..>21247)
                     /locus_tag="VC394_001038"
     CDS             complement(20993..>21247)
                     /locus_tag="VC394_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994513.1"
                     /translation="QLSKDKAEVYFTRLYNQHIAWVIIANVMTEYVIKFRKSATSFEE
                     AWDALDYQRTTEIVFRAVNGLPCSEKDSGELETYLSEVSA"
BASE COUNT         6138 a         3838 c         4342 g         6929 t
ORIGIN      
        1 tctcttctag tgtccaagat gttttgattg gctagtttac gaagatggtg ggaggcagat
       61 gccatactga gatttaataa acaggctata tcgcagacac agagttcttc gacggcaagg
      121 agataaaaga tgatatttat ctgtttatta tcggtaaatt ttgataaaat acgaagtgat
      181 ttttggactt tttccttttc aaggtagttc gttgcggttg taacattttg ttgatttata
      241 acacctactt gacagatact atcttttttc ataatttttt ctcctagcct aacacagccc
      301 atagcatgtc aaagctgttg ttttcaatca ggatatacat ccccaatcct aaatagacaa
      361 cggcaataaa ccatctgcta tatttttcca aagtttctcc aacagaaggg acttgtgcca
      421 atttttgggc agaaaaaacc aagagataaa tcatgactag aaaggtaagt aaagccacta
      481 tcaaattcgc taaatttaag gtagtaaaat atgggacaaa gacaccaata ttgtcagcac
      541 cacaacttgc aaaagtaatc atagcgacta gaaaaatcag gtttttatta tctttgctca
      601 aaccttcttt ggcaatagct tctccatcag aatctcctaa aagcaaaact ttgaggccta
      661 ggaaaattgg aatcaaaccg agcaaaccta aaatctcttt actaggaata taatctaaga
      721 caaatgcaaa aagcaaactt agcaatatta gactaacaga gcctagaaat tgtcctaaat
      781 agatgttaat gatgtctttt ctgctttttc ttttggcaaa aaataacatt aggataataa
      841 gtaagtctac ggctgtccca gaatacagga ttattgaagt aacaacattt tgaatcataa
      901 aacacctcat tcaaatatat ttttgaatga attttaacat taaactttgt agatgtcaat
      961 ttcagctaca ccaaaatata gataagaagt tagtgtacca aatataaaaa agccctgcca
     1021 tccaaatgat aacagggctt aacttcaata tccaaatgat atattcatct aaaaaaggta
     1081 gagtaaaagg tagagttctt attgatttat attacaatgt aatgaacttc aaaaattcaa
     1141 aaatcgctta aaatcaacgt tttgacgtct aatgacgtgt agtgaacccc ttacttaacc
     1201 tctgtaaatg taacgtgttt acgtaatttt ggtgagtatt ttttcaattg aagacggtct
     1261 ggagtgttac gtttgttttt tgaagtaagg tacaagcgtt caccagattc tttatgttca
     1321 agtgtaatat ttacgcgcat ggtatctccc ttctattatt tagcttcgtt agctttagcg
     1381 attttacgtc ctttgtagta tcctttaagg gatacacggt gagaacgtga gtaatctcca
     1441 gtagtttcgt cgaattgtac agatggagct gtcaatttgt agtgtgtacg acgtttgttt
     1501 ttcttcgctt ttgacgtgtg acgtgcaggt actgccattt tcttattctt cctttcgatg
     1561 tgtaagtttt gattttcatc aacttcacta tgataacata tcttttttgt aaagtaaagt
     1621 tacttgacaa aactttttat ttttacaatt tcatggtgaa aatactatca aactaaagca
     1681 aatgatatat gcttgtttaa aatataagaa aaaatggtag aatgtttaaa gagaaaacta
     1741 atcaaagagg taaacttatg aaacttcaaa aaccaaaagg aacccaggat attcttcctg
     1801 aggatgctgc gaaatggcag tatgtggaat ctgttgcgcg tgatacgttt agccaataca
     1861 attatggaga aattcgtact cctatgtttg aacactacga ggtaatctcg cggtctgtgg
     1921 gtgatacgac tgatattgtg acgaaggaaa tgtatgattt ctatgataaa ggagatcgtc
     1981 acattacctt gcgaccagag ggtacagctc cagtggtacg ttcttatgtt gaaaataaat
     2041 tgtttgcgcc agaggtccaa aaacctgtca aactttatta tatcggttcc atgttccgct
     2101 acgaacgccc tcaagctggt cgtttacgtg aatttcacca aattggagta gaatgtttcg
     2161 gtgctgctaa tccagcaaca gatgttgaaa ccattgcgat ggcctatcac ctctttgaaa
     2221 aattgggtat taaggatgtc acccttcatt taaatagttt ggggagccct gagagtcgtt
     2281 cagcttatcg tcaaaccttg attgactact taacaccaat gcgtgatcaa ttatctaaag
     2341 atagtcaacg tcgcttggat gaaaacccac tgcgcgtgct tgattctaaa gaaaaagaag
     2401 ataaactagc cgttgaaaag gcgccttcta ttttggatta cttagatgag gaaagtcagg
     2461 cgcactttga ggcggtcaaa gatatgcttg aagcacttga cattccttat gtgattgaca
     2521 ccaatatggt acgtggcctt gattactata accacactat ttttgaattc atcacgtctg
     2581 tggagggctc tgacttaacc atctgtgcgg gtggacgtta tgatagtttg gtaggttatt
     2641 ttggtggacc agaaacccct ggctttggct ttggtcttgg tttagaacgt ctcttgatga
     2701 ttattgaaaa gcagggcatt accttgccaa ttgagacaga gatggatgtt taccttgctg
     2761 tgttaggtga tagtgctaat agcaaagctt tagaattggt tcaagccatt cgccgtcaag
     2821 ggtttacagc tgaacgagat taccttggcc gtaagattaa ggctcagttt aaatcagccg
     2881 atacctttaa agcaaaattg gtaatgactt taggagaaag tgaagttgaa gcaggcaaag
     2941 ctgtgattaa gaataatcgt agtcgtcaag aagtggaagt cagttttgaa gatatgatga
     3001 caaactttgc aaacatctca gagcaattat tgtcttaatg attttattgt cgtcataagc
     3061 ttttgttgat atgattgttt agaagataat cactaaaaca ggtgtaactt ccctttttag
     3121 aagggagttg cacctgtttg ttttctgtaa ggggattgat tagctttctt agtattctgt
     3181 gaggacacct gatatgtcaa aatgctgacg actcagtatt cttgctttat ctttagaaaa
     3241 actcttttta aatgggtggg gtttttaagg gctttttgct ataatagtag ggaatgaatt
     3301 aattgattta tatagtgaaa ggaattggaa acgagcaggt ttcacaggat tctgtttgtt
     3361 ttagggaatt tatttatgaa acgtagcatg tatgcagggc gtgttcgcga agaacacatt
     3421 ggaacgacaa tcacattgaa aggatgggtc agtcgtcgtc gtgacttggg tggtctcatc
     3481 tttattgatt tacgtgaccg tgagggtgtg atgcaattag tcattaaccc tgaggaagtt
     3541 tccagtgatg tgatggcaac tgctgaacgc ctacgtagtg aatatgtgat tgaagtggaa
     3601 ggttttgttg aagctcgaca acaagccaat gataagttgg cgacaggaat ggttgagtta
     3661 aaagtatctg ctttgaccat tttaaacaca gctaaaacga cgccttttga aattaaagat
     3721 gacgttgaag taagtgatga cacgcgttta cgttaccgtt acttagattt acgacgtcca
     3781 gaaatgttag aaaacttcaa gctacgtgca aaagtgactc attctatccg taattacctt
     3841 gatgaccttg agtttattga tgtcgagaca cctatgttga caaaatcaac tccagaaggt
     3901 gcgcgtgact atctggtacc aagtcgtgtt agccaagggc atttctatgc tttgccacaa
     3961 agtccgcaaa ttaccaaaca gttattgatg aatgctggct ttgatcgcta ttatcaaatt
     4021 gtgaaatgtt tccgcgatga agatctacgt ggtgaccgtc aacctgagtt tacacaagtt
     4081 gacttggaaa cgtcattcct ttctgaacaa gagattcaag acatcgttga aggcatgatt
     4141 gctaaagtga tgaaagagac aaaagaaata gatgtaaccc taccattccc acgtatgagc
     4201 tacgatgtag caatgaatag ctacggctct gataaaccag acacacgttt tgagatgctt
     4261 ttacaagact tgacagtaac tgtcaatgga attgacttca aggttttctc agaagcacca
     4321 gctgtcaagg cgattgtggt gaagggaaat gcagatcgat actctcgtaa agatattgac
     4381 aaattaactg agtttgccaa acaatttggt gctaaaggcc ttgcctgggt taaggtgaca
     4441 gatggtcaat tggcaggccc agtagccaag ttcttgatag ctattgagac agagttgtca
     4501 agccaattaa aacttgctga gaatgatttg gttttatttg tagcggatac tcttgaggtt
     4561 gctaataata cattaggtgc gcttcgtaat cgtattgcca aggaccttga catgattgat
     4621 cagtcgcagt ttaatttcct ttgggtggtt gattggccaa tgtttgagtg gtcagaagaa
     4681 gaaggacgtt atatgtcagc tcatcacccc ttcactcttc caacgccaga atctgctcat
     4741 gagttagagg gtgatttggc taaggtacgg gcaattgctt atgacatcgt tttaaatggt
     4801 tacgagcttg gtggtggtag cctgcgtatc aatcaaaaag agatgcaaga gcgcatgttc
     4861 aaggctcttg gctttaccgc tgatgaagct aacgaccaat ttggtttctt actagaagcg
     4921 atggactacg gctttcctcc tcatggtggc ttggcaattg gtcttgaccg ttttgtcatg
     4981 ttgcttgctg gaaaaggcaa tattcgagaa gttattgcct tccctaaaaa taacaaagcc
     5041 tctgatccaa tgacgcaagc acctagtctt gtttctgaaa accaattaga agaactcagt
     5101 cttcaaatag aaagtcatga ttaagaaaac aacctataag aaaaaagtta aatatgtcat
     5161 tagtagaggg gccaagaaag ttggcctact ccacgctcta agaagtattt caagagaaaa
     5221 atatgcagag aagatttcgg cttctctgct ttatggcatt ctctctagta ttgctgtgaa
     5281 ttttttcttc cagcctgggc atgtttattc aagtggagca actggtctag cacaggtttt
     5341 ttcagctctt agtcatcgtc ttttaggcta tgattttccc atcgcctttg cgttttattt
     5401 gattaatatt cctttgctta ttttagcttg gtataaaatt gggcatcaat ttaccatttt
     5461 tacctttatc acagtcagca tgagttcttt ctttattcaa atcatgcctc aagtgacgct
     5521 gacgactgat cctcttatca atgctatttt tggtggtttg gttatgggaa tgggaattgg
     5581 tacaggtctc aaatcacgta tctctagtgg ggggactgat attgtcagtt tgacccttag
     5641 aaaacgaaca ggcaaggatg tgggcagtct ctcattgatg gttaatggtg caattttagc
     5701 ctttgcaggg attttatttg gctggcagta cgccctttat tctatggtct ctatctttgt
     5761 atcaagtcgt gttacggatg ccattttcac caagcaaaag aaaatgcagg caactattgt
     5821 taccagccat ccagagcgtg tgattcatat gatccataaa cgtctgcatc gcggggtgac
     5881 cagtatcaac gacgcagaag ggacttacaa gcatgaacaa aaagcagttt tgattaccat
     5941 tttgacatgt gaagaatacc cagaattcaa atggctgatg ttaaaaacag acccacaagc
     6001 ctttgtttca gtggctgaga atgttagaat tatcggtcgt tttgtggaag atgattaaga
     6061 aaaacacagg aggtgtcatg tcaattgacc aacgtttaca gttaaagaaa ttttgcttta
     6121 ttattttagg agctgctatc tacgcctttg cttttgttta tttttacatg gctaatcgca
     6181 ttgcagccaa tggtttagct ggtctcaccc tagttggtaa ggcacttttt gagattgatc
     6241 cttctttgat aggctatttg attaatttgc ctttggtctt gctgggagct agatgttttg
     6301 ggaaaagagc catggtttac acagtaacgg gtgttttatc actttacttt tttgtgtggc
     6361 tgtttcaacg ctttcctttg gtcgttgact tggaccatga caatttagtc gtttccttga
     6421 tggctggaat tattggtggt ctaggtggcg gttttgtgtt tcgcaatggt ggaaccatcg
     6481 gtggagcaga tattattgct aagttattag aagacaagtt aggactacag ctgaatcaag
     6541 ctcttttagg cattgatctt tttgtcatga ttgtctcttt gacttatatt tccctgccgc
     6601 aaatgatgta tgctctgatt gctagcttta tgtatggtca aattgttcgc ttggtacaac
     6661 aaggtggtta ttcagcccgt ggcattttta tcgtttcaga acaagcggag aaaattgccc
     6721 ggttcatcat ggaagaattg ggaaggggag ttacttatct aacaggtgaa ggagcctact
     6781 ctggtcgccg taaaaaagtg atttatgtgg ctttggggcc aagagatatt agggaattaa
     6841 aagctttttt aactcaggtt gatcctaatg cctttatcac ttattttgat gttaatgaag
     6901 ttaatagccc agaattttta actattaaaa gcaaatacca taaaaagaga aaataaatga
     6961 gcattagagt tgcctgcgaa gaatagtgag gataaaggat ggcacatcac gataaattga
     7021 ctaaactact aaaactgttt ttgattgccc taggtgtagc catttatacc ttcggttttg
     7081 ttaattttaa catggctaat gctttggctg aaggtggtgt ggcaggaata accttgattt
     7141 tacacgcgca ttttgggatt aatcctgctt actcttctct cttatttaat cttccacttt
     7201 ttattttagg ggcaaagatt tttgggaagc gttctttggc tctaaccatc tatggaacag
     7261 ttctgatgtc cgcttttatc tggatgtggc aaaaagttcc tatcgaactt ggcttggaaa
     7321 atgacatgat gttggtggct gtagtggcag gtctcttttc agggattggg agtggcattg
     7381 tttttcgcta tggtgcaacc acaggtggaa cggatattat tggtcgcatt gcagaagaaa
     7441 aatttggagc taagctaggt caaactttgc ttttggtgga tgctcttgta ttgacagctt
     7501 cgttgactta tgtggattta aaacacatgc tttatacttt ggtggcaagc tttgttttta
     7561 gccaaatgat tagtgtggtt caaaatggtg gttacactat ccgtggaatg attattatta
     7621 ccaagcattc agaggctgcc gctcaagcta tcctaaccga aatcaatcgt ggagtgactt
     7681 acttgaaagg tcaaggagct tactctggta atgattacaa tatcatgtat gtgaccttga
     7741 acccaactga agttcgggaa gtcaaacgta ttttagctgg tttggatcca gatgccttta
     7801 tctccattat tgatgtggac gaagttatta gctctgattt taaaattcgc cgaagaaatt
     7861 atgataaata aaaatacagg tcaaacagcc tgtattttta ttttggtccc ccacttaaga
     7921 aactaccaag cataatagct gatatagcag ttcccttgta atggctagct tttgttttta
     7981 ccaagcgact aagatcaaat atgcttttag gagcatcaaa ttgatgtgtt aagatgtatt
     8041 gattgataag tgagtcaatt tttgtagcta ggagtgagct catcccctct ttttcaagtt
     8101 gactggcgag ctcgaagatc gcttgacgaa aataatcatc ggcttgtatt acttgttcgc
     8161 tataaacttt actaagagca tctaatattt ctttttctga tggcatggca ttctcctgta
     8221 acatattgtg ctattattat aatataaaaa actaagactg tcataaatgt gacagcctta
     8281 gtaatgttat tacttacatt tcatttggtg cgtcaacacc gagtaagcga agcgcttctt
     8341 taagaacagt agcagttgca tagcagagag ctaagcggtt atcacgttct gagttgtcgt
     8401 caagaatgcg agtgtgagca taatatttgt taaagctttg ggctaagtta atagcaaatt
     8461 tagccataat agaaggctca aagttatctg atgttcgctt gataatgcgt gggaaatctt
     8521 ggataagttt gataatttcc cagctttcag catcagctaa actgtaagtg gtggttgcag
     8581 atggcgtaaa gtcagcttta cgtagaatag attggatacg agcatgagca tattgaacgt
     8641 aaggtccagt ttctccttca aaggaaacca tggcttcaag gtcaaagtca tacccattca
     8701 tacgatctgt tttgagatcg taaaacttga tggcaccgac accaacagag tgtgcaacgg
     8761 cttctttgtc agcaaggttt ggatttttcg cttcaatttg agaagctgca cggttaatgg
     8821 cttcagcaac cgtaggttca agaagaataa cattaccttt acgtgttgat aatttagcac
     8881 cgccttttgt gacaagtcca aaagcaacgt gtgtcatatc atctgaccaa tcgtaaccca
     8941 tttcttttag cactgctttt aattgtttga agtgagcagc ttgctcatta ccaacaacat
     9001 aaacggattt agcaaagtca taagtgcgtt tacggtaaag tgcggcagct aggtcacggg
     9061 taatgtaaag agtagcgcca tctgattttt tgatcaaggc agggtgttca atgccatatt
     9121 tttcaagatt aacaacttgg gcacctttag attctacaag aaggttttta gcttctaata
     9181 agtcaagaac ctcatccatt ttgtcattat agaaggcctc accgttgtaa ctatcgaagg
     9241 tgacatgtaa ttggtcataa agtcggttga actcaagtaa actttcatca cggaaccatt
     9301 gccataattc agtagcttct ttgtcgccat cttccaattt acggaaccat tcacgagctt
     9361 cttcatcaac ggtgggatca gtttcagctt cagcattgat acgaacgtaa agttttaaga
     9421 gttcatcaat aggatgtgcc tgaacagcag cttcatctcc ccattttttg taggcaacaa
     9481 tgagcatacc aaattgttta ccccagtcac caaggtgatt gatttttaca ggtttatagc
     9541 ccattttggc aaaaatgtga gcgagactat caccaataac tgttgagcgg aggtgaccga
     9601 ttgaaaatgg cttagcgatg tttgggcttg acatgtcaat agcaacattt cgcccttgac
     9661 cttcgtcttg ctgagcatag tctgaacctg ctgtaatcac ttgttcaagg acctgtgagg
     9721 aaatcttagt tttatcaagg aaaaagttga tgtaagggcc gacagctacg actttttcaa
     9781 attggctctc atcaatctgt tcagctaact cgctagcaat catttgaggg gctttgcgaa
     9841 ggacttttgc taaactaaag gctggaaagg caaggtctcc catgtcagaa ttttttgggg
     9901 tttcaagcaa gttaaaaata gcatcttgtt ctaattctgg aacaacttta gctatttcac
     9961 ttgcgattag agttttagta tccatacagc gtctcctaaa aattgtttgt caccctattc
    10021 taacatattt ctttaaaata aacgaggaga ggattgataa attatactga aactttcaag
    10081 gaaatgaatt gcttgaggtt gttgtttttc tgaaaatgtt ataaaaagag aaatgaggtt
    10141 tcttttcaac agttttcaag tgaaattcta gttgaatttg ctaaaaaatt gctgaaaata
    10201 gctgggcgaa gatgaaataa tacacttttt ttgttataat ttgagaggat aattatacaa
    10261 ttggggtgat atatcatgaa taaaatggag cgtcaacagc agattaagcg aatcattcaa
    10321 gcagagcata ttggcacaca agaagatatt aaaaatcacc ttcaaaaaga aggcattgtg
    10381 gtcacccaag caactctatc tcgggactta agagaaattg gccttttgaa attgagagat
    10441 gaacaaggca aactctatta tagtttgtct gaacccgttg caacaccatt tagtcctgag
    10501 gtgcgttttt atgttttgaa agttgatcga gcaggtttta tgctggttct ccataccaat
    10561 ttaggagagg ctgatgttct agctaatttg attgacaatg atgccattga agatattcta
    10621 ggaaccattg ctggtgcgga taccttgctt gtgatttgca gagacgaaga gattgctaaa
    10681 cgctttgaaa aagatttggc tgcaggccta tgactagcta ttaagagtct ttgacacaat
    10741 tgattcttgc tatccatgaa ttctagtatc gttgcttata aagaagtgca gcaatagctg
    10801 tctaagctcc gtgattttaa aaagtaagct tatcagatga aacgctatca acaagatgcc
    10861 ctgcttttca aaaaaaatag ataaagaaaa ggctgcgaca gtatctgcaa gcagggcaaa
    10921 agaactagaa gataggctca gtcatcagcc attaattgat gattatcgag aaaagatgca
    10981 agatgcaagt gatgtgattc agtatatcac caaacgtata gaagatcagt taaataagga
    11041 gttaacaaat ggcaaaaact aacatttctc ctggaatgca acagtatctg gacatcaaaa
    11101 aagattatcc agatgctttt ttgcttttta ggatgggtga cttttatgaa ttattttacg
    11161 aggacgctgt caaagcagca caactcttag aaattggttt gaccagtcgc aacaagaatg
    11221 cggaaaatcc aattcctatg gcaggcgtgc cacatcattc tgcccaacaa tacattgatg
    11281 tgttaattga gttgggttac aaggttgctg tcgcagaaca aatggaagac ccaaagcaag
    11341 ctgttggggt ggtgaagcgt gaggtcgttc aagtcataac tcctggaacg gttgtggatt
    11401 cagctaagcc agatagcgcc aataactttt tggtagctgt tgactttgat ggttgccgtt
    11461 atggattggc ttatatggat gtgtccacag gtgaattttg cgtgacagat ttggcggact
    11521 ttacgagtgt tcgtagcgaa atccaaaacc tcaaggcaaa agaagtctta ctaggttttg
    11581 atttatctga agaagaacag acgattttgg tcaagcagat gaatttgctg ctttcttatg
    11641 aagaaacggt ctatgaagat aaatctttaa ttgacggcca attgacaacg gtagaactga
    11701 cagcggcagg aaaactcttg caatacgttc acaaaacaca aatgcgagaa ctcagccact
    11761 tgcaagcatt ggttcactat gagatcaagg attatttgca gatgtcgtat gccactaagt
    11821 caagtttaga tttggtagaa aatgctagga ctaataaaaa acatggaagt ctctattggc
    11881 tgttagatga aaccaagaca gctatgggga tgaggctttt gcgctcatgg attgatcgac
    11941 ctttggtttc taaagaagct attttagagc gtcaagaaat tattcaagtt tttctgaatg
    12001 cttttattga gcgaaccgat ttaagcaata gtttaaaagg tgtttacgac atcgaacgct
    12061 tatctagtcg cgtgtctttt ggtaaggcaa atccgaaaga tttacttcaa ttggggcata
    12121 ccttagccca agtgccttat atcaaagcta tcttagagtc ttttgacagt ccttgtgttg
    12181 acaaacttgt caatgatatt gacagtttgc ctgagttgga atacttgatt agaacagcca
    12241 ttgatccaga tgcaccagca actattagtg aaggcagtat tattcgtact ggttttgatg
    12301 agcgcttgga ccattatcgt aaagtgatgc gagaaggaac aggttggatt gcggatattg
    12361 aggctaaaga gcgtcaagca agcggcatta ataacctaaa aattgattac aataaaaaag
    12421 atggttatta ttttcacgtt acgaattcaa atcttagctt agttcccgag cattttttca
    12481 gaaaggcaac tttaaaaaat tctgagcgtt atggaacagc agaattggct aagattgaag
    12541 gtcagatgtt agaggctagg gaagagtcat ctagtttaga atacgatatt tttatgtgta
    12601 ttcgagctca agttgaaacc tatattaatc gtttacagaa actggctaaa attttggcaa
    12661 cggtagatgt tttgcaaagt ttagcagtcg ttgctgaaac caatcattat atccggccgc
    12721 agttcaatga taatcatgtg attacaattc aagaaggtcg tcacgcggtt gttgaaaagg
    12781 ttatgggagt gcaggaatac attcccaata gtatctcttt tgaccaacag accagtattc
    12841 agctgattac aggtccaaat atgagtggta agtcgactta tatgagacag ctggccttaa
    12901 cggttatcat ggcccagatg ggttcatttg tggctgctga ccatgttgat ttacctttat
    12961 ttgatgcgat ttttacgcgt attggggctg ctgatgattt gatttctggg caatcaacct
    13021 ttatggtgga gatgatggaa gcaaaccaag caatcaaacg cgcaagtgac aactctctta
    13081 ttctatttga tgaactggga cgaggtacgg caacttatga tggtatggct ttagcccagg
    13141 caattattga atatatccat gatagagttg gtgctaagac catatttgca acgcattatc
    13201 atgaattgac agacttgtca actaagttga caagtctagt caatgttcat gtagcaacgc
    13261 ttgaaaaaga tggcgatgtt accttccttc ataagattgc tgagggaccg gcggataaat
    13321 cttacggtat tcatgtggca aaaatagcag gactgccaaa atccctatta aagagagcag
    13381 acgaagttct gacccgttta gaaacacagt cacgatctac tgagataata tcagtccctt
    13441 cacaagttga gtcaagcagc gctgttagac aggggcaatt atcccttttt ggtgatgaag
    13501 agaaagctca tgagattagg caagcactgg aagctattga tgtcatgaac atgaccccgc
    13561 ttcaagcaat gacaaccctt tacgaattga aaaagttgtt atagtctttc agctgaaaat
    13621 gaaaaagatg ctttctatat tgaaggcatc tttttgttct gtcaaaaacg gtccgaggcc
    13681 ttcggctatt tattaagtgt gttataatag tccataagaa tgcgaggaaa ttatgacaaa
    13741 cattattgga cttggattgg cagtctttac agggtattga cagtccttaa gcattgttat
    13801 gactgtgttt ttgaaaaaat ataggtttta agtttcctta gacttccctc aaaagtccac
    13861 aaaaaggtga acaaaaaaag acctttacag gtcctttaca caatgagttc agcaggcaag
    13921 aactagcgtg gtttaaatac tacgcttttt agtttgccct atggcttatt atagcacgat
    13981 tagtgtcatt gaggaaaaaa ctaccctctc aaccagtctt taaagttcca ccattggtct
    14041 tggatgctat cgctcaagcg ttgatgccaa gtttttttgg attcttcttc gatagaacgc
    14101 ttgaataaca cctccgcata ttgttgctct tcggcatctc tctctttttg aagacgttca
    14161 ttatgtgttt ttccatcctc aaatacaagt tgcccaaagt atagattccc gtcatccgca
    14221 aaagataagg taactttgtc tccttcagca agtggttttt ccaattgaac tgtgtagttg
    14281 cctcctccat cagcacgatt gccacttact gtccatttcc ttccttcgca gtttggatta
    14341 tcttgtttct ccaaaataga gcaaggaatt tcataagttt tgttttcggt ttttttaatt
    14401 ttattaccat tacttgaagt tacaaaacca taaacatacc catagggatc tagattaaca
    14461 ttaatggttg tatctcctgt tttaaatggt gctacggtta ctccaggtaa gtgcgattgc
    14521 cctatagttg taaacaaatc gccactttca ttagctctga cacttatagc acatattgat
    14581 attaacaaag ttaacatcat aactaatttc tttttcacta tattctcctt aaaattaaat
    14641 atcgaactcc acaataccag ttaatcagtc aaaaaatcca ttctactttt tgatatttta
    14701 tgtctaattt gacccgctaa gtcagatttg caaacgatac caagtgattt tcttctaatc
    14761 attatttttt agatggatat gttaattgta ttagagacgt attaatctta aaccattgtc
    14821 ataagcgaac attatttttt agcgccccgt ctctgtaaaa ttccacaaac tcaagcaagg
    14881 ctcttttctt agattcataa taccagcttt gacttctgtt aagttcttcc ataatgtctt
    14941 gttgagtttt ttcctcactg atcaagtaac actcaattag tatttgtcta tattctactt
    15001 tagacagttg attaatggca tacctaatag cgtctagttc ctctagggcg cattctcggc
    15061 ttatttcaag gtgttttctg cgcgtgggat ggtactctat atcaaactgg taaagctcgg
    15121 tataagttaa atcaaggcta ttagcgatac gttgccatct atgaaattct ttcagcttac
    15181 gaatagcgtt cttcttgctc atctaacacc tctaaagctt ctctatgtag tttgaacaca
    15241 atgtttcttg agtaacctag tttatcgagt atctcgtccc atgataagtc atccacgtat
    15301 ctagccttaa taacagctat ctgtctctca tcttggagcg tggcgatcat ggctagtctc
    15361 ttatcacgtt ctttagctaa atataagagt tgtttagctg tacctttttc gatatgtctt
    15421 attgcttcag gattatgaaa agcatttatc agctcgatgt ctttatctcg ttgttcctca
    15481 aataaggtta ttaaagcgaa aagtggtttc aattctttaa gttgttcttt agcgctcatg
    15541 gtcttttctc ctggttatgg tataatttat ttaagcttaa atttaaccaa gggggcgttc
    15601 gtatggacgt ctttttgttt tgttcgtttt gtaaactaga tattttttgt agtacttcgc
    15661 ttttcgcttc taaaatcgtt tctaagcact ttttagagtt tatagtataa atcatcaact
    15721 tagtagctag aagcgctaaa acaatgtttt attttagtct tagatggtta ttctttgtgg
    15781 cagggttatg ggctaaaatg agatgtcaaa tcctttccta tcaagctttt taatcttcgt
    15841 caaaaatttc aaaaagggaa ttttttgcac ggaaaagggc gcgttcttaa gtttccgaac
    15901 aatatagtcc cgtttaaaaa tgaagggggt agtttccgaa tattatagcc agtacctgtc
    15961 acccttatcc atatagtaat gaaatcttct ccagtgataa aggtatttgg ttaatctcat
    16021 gtactttgga cgtttaggaa agtcatcacg actataatat ccatgaatgt gtcttgcttc
    16081 tggatttact ttcaaacact ctttaaaggc taattgccag tagtagcaac agtctgtctt
    16141 gcttcgattg agcgtggctt gatgaacctt ctgacaagaa ccacaagcaa aggcatgaga
    16201 agccttaaat aatttccgac aacgtctctc acagtcagga cacacgaaaa agtaacgttt
    16261 accaccataa gttcctggaa tcgtttcaag caataggcct tgaccattgt agtgaatcac
    16321 tagcccatct aggtctatgc ggatagcttg gtcacctatt gatcctgtaa ctcttgtctt
    16381 cccctgtgtc ttcattggtt taatgatatt ctcaatagat agttctaaca ttatttctcc
    16441 ttaaaactca aacaacccaa aactattgat aaaacgacaa aaagaggaaa aaccctctga
    16501 atgtctattt aacgagtaac tgaccttcaa caaccatatc atacaaatgg ttaaaggctt
    16561 gactgataga ctcaaggatg gcccctaagt cttctggcgt catctcttta taattcatag
    16621 agaggtgttc agccagttgg ttgtgatcgg agatgaaggc tataagtgtg tctcgattat
    16681 taactttccc ttgagtactt ttagataaag aaaccacgcg ctgatgatca agttcttcat
    16741 ccgtcatatc ctcaggttgg ttgtaatagt ccttgaagct gtcacagata cgcttgaaga
    16801 ctttgtttag ttttctgtct tcaacatatt ttaagactaa ctgattagca tgaccacctt
    16861 ggtcatcatt gtgataagtt gcgtcaatca ctggttgctc ataagtacca gtcatatagc
    16921 ctaaaatagc atgacaagcc acttgtgcgg tgtcaaagtc tttaaacgtg tagtggaatg
    16981 tgaatgtctt tggtgtgtct gaaaatgttc tcatgctatt tctcctttgt gattgctata
    17041 atgtctgata aattgataat ggcagaagga gatgctaccc agtttggttg ttctccagat
    17101 aaaagatact tgaccaactc attataaagg gtgcggtctc cttgtatggt gatggtgtta
    17161 ccacctcgtg tgtgtaattt tagtttcata tcagttacct gtacaagacc agtagtcctg
    17221 ttcttgaatc catgtcttca taattgccat aagtggcttc ttggaattta atgtctatca
    17281 cagataccga tagggtaaag agattgaccc gatgttcaaa atcatctagt gattcattgt
    17341 gtttttgata aaatagtttg attttcatgt tttagctcct ttctcaacag gtatcgcaaa
    17401 gccacagtca aagacccatt ggtaaccacg tcgtgttagt tctggtctgg taaaatcaca
    17461 atcacccggt ctagacttta tgttgaaatt aattttggtg ataaagtcat aaccgcattg
    17521 accattagga agttccaatc ggtatagctg gtcgtttggc aaatcatcat aattgtaacg
    17581 cattttcttt acgccatgat ttgtatagtt aggattcata tgtccctctt ttctctctaa
    17641 aatgtgtgct ttttgttttt ttaaaaatca aatagcacag caaaaagcac agggataaag
    17701 ccagtggtat caagggggtt gggcactttg tgcttttagt agcattaatt tgagcaaaag
    17761 actttctttt tttgcacgcg cactattagt tataaatatt attatctata atattaaata
    17821 ctactaatac tacaatagag tataaagcct ataataccaa ggttttagct tgtatcatta
    17881 cgtgtagttt ttatgtagca ttagtgtagt ttttaaactc gttcatagta aggtacggga
    17941 gttccacctt taaatagtct ttttcttgcg ggattctctc cttttttcca gccgtcatca
    18001 ttatctaagt aatcacgtat tttttgagaa ataagggcct taccaccttg cgttggtttt
    18061 tggttaaatc ctagataagc gatatggtta ggacttgtca cttggagtaa gcaatctgtc
    18121 tgtgcggaag ggtagtcact atagctttga gcattatcta acggctctcc tagctgtttg
    18181 aggatgtatt gccgttgttc atactgtgat aagctatccc aaccttcaac aatttgaaac
    18241 tcattcagta attgatcgat aatttctttg tcgacgtctt caaccttata atcttcttga
    18301 atgtctgcta actgattcat taactcttta gatggcgtta gtggttcata attgttaaac
    18361 catactttgg cttcagcgag tacctgtaag aaataatctt cttccacctc cataggatgt
    18421 tttttcacgt cattgatacc acattcaata gggaaaaagc gtctttctgt tccactatcc
    18481 ttaagaaaag attttttatt agctgttccg ataaagacac agtgccttgg atgaggagtg
    18541 gctttacgtt cataaggttc acgataagta tcactatctg aggaaatgaa gcttttaact
    18601 gtttcaattt ctgcctttga catgcctttt agctccccta gctcaataat ggcattggct
    18661 tgtatcttct gataatcact atcattttta ccaaacttga tttctgaatc agtgtggtag
    18721 ctagggagta gtcgcttagt aacggtgctt ttcccagttc cttgtctttt atcaatgaga
    18781 ataggaacga cttcaaactt tactttacgg agataaattc tagccatgag acctgttagc
    18841 catactttgg caatttctct attataggaa ttatcagcac agcctaatag atcaataaag
    18901 tagcgttctc ctctagcttt accatcccat ttttgacttt caatacgctg tttaatgggg
    18961 tgataagtgt tctttttagc taaagcggta atagctacct ctatatgttc tttacgaggg
    19021 gtaaaccgat atttttcatc aatgaatgca atacaaaggc tggtctgctc gttcgtccat
    19081 agcccttttt ctttagacca aggaactgct ttagtgattt caatagtttt ttcaaattcg
    19141 ttgtatttaa tacctgtata gatattttcg taaaattcaa aaaccttacc gacattgtag
    19201 gggctactaa tgacatattc tttgtctcct ctaccttttc gtgtcctgaa agcaggggca
    19261 aaagcaggtt gagtggcttg cgatagttta ttttgatagt ctttcaattc ttctttgtct
    19321 atggcttgat tcctctcttt cttaattctt tatcaagtat gcttctaaag gttgtatcta
    19381 tctcatcaat gggtagtggc ttagttgtca cgctgttagc tatttgtacc agctcataag
    19441 ccgtctctaa atcacaatcc acccatttat taaatagcaa gccaacaaac ttagttaagg
    19501 ccacgttgcg cccgccttcg tctccaaaac cattaaacaa ggtatctatg accctcatgg
    19561 taatagaacg ctgacttcta gggcgtggcg tgtaagtagt aacaacttgt ctgtttggcg
    19621 tgctaccatt tttaggaaca ggataatcaa gaccatggtt cacatagcgc tgatagtcct
    19681 ctgggtcgcc tgttgtaacg ggtaagcctt gtaattgcga ccaggtaaga ctagctaaat
    19741 caaacggcag tccaatctta tcggctatct ccttgaccac ttgtttataa gttgcttcag
    19801 tcatcacgtc actaggcttc atgacaaggc gataacgggg cttctcgggg gtgtgtttaa
    19861 tcgttggata aataatataa ctatactccc aaagcgtctg agaaacgatt ttaggtaggt
    19921 tgacgcctgt ttctatctcg tcatagtcaa gaaaaatcaa atcgcgataa actaaactag
    19981 cattattgcg cttatagcta ccgtttttct ctgctgtgac cttgccactt aggcagtagg
    20041 gggcttgtgt tcgcttgtat tcttcaatat caatatcctc aggcggtttc aaaggtctaa
    20101 actgagcaac atagtcaaat ggttctaaag gtcctttgta ggggtacaaa taagagctaa
    20161 agcctcttgc ttcataaata gccatctaca catttacccc caaaaagata agaatatcac
    20221 tgaccttgta ataatgtttc ctggtgtctt ctagtggtgg ttggtatcgt cttaacccag
    20281 cattttccca ccgttttagg gttttacctt tgatatttaa ttcctctttg acttgttcgg
    20341 ccgtgatcaa ccctaaaact cttggtttag gtttctggta ggcttccaaa aagtgattaa
    20401 aagcggtcag gttttgttct aagagtttgg cttcataatc ttgactaaat acgttcatgc
    20461 ctaacctcct ttgagtaatt ccttataact ggttaaatcg gcattcaata acacacttag
    20521 gcgttcctgt tccttttgta cttgattata aaaggcttta gcaccatcta gtaattcttc
    20581 tttgttagct gggataaagt acccacgatt gaatccgtgt ctaatgccga taatagggac
    20641 gttatagcgc gtgattaagc tactgatgat actttggacg gagcgttctt caagtttcag
    20701 tattaagcta atctctgccc ctgtaatggg gttgtctgct ccaaccttga tcagattaag
    20761 gacacgtcta taattctctg gtagtgtcat tcagttcctc cctaattgta ataatggttc
    20821 tgtgcttgaa tataagcccc atagtttgcg ttctgacgtg gtttaggtgc gtgggtatct
    20881 tctggtaagt caatctctat taacggctta gaacggctaa gaagaagccc taagagacct
    20941 aaaacaaaga atagaataag cgtctgtgtt ggtgtgaggt taagttcttg catcatgccg
    21001 atacctcact taaataagtt tctagttccc ctgagtcttt ctctgaacaa ggtaaaccgt
    21061 taacggctct aaagacaatc tctgtggttc gttgatagtc taaagcgtcc catgcttctt
    21121 caaagctggt ggcacttttt ctgaatttaa tgacgtactc tgtcataacg ttagcaataa
    21181 ttacccaagc aatatgttgg ttatatagtc gagtgaaata gacttcagct ttatctttgc
    21241 tgagttg
//
LOCUS       DATXQK010000024        21090 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.024, whole genome shotgun sequence.
ACCESSION   DATXQK010000024 DATXQK010000000
VERSION     DATXQK010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21090
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.024"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(525..911)
                     /gene="rplQ"
                     /locus_tag="VC394_001039"
     CDS             complement(525..911)
                     /gene="rplQ"
                     /locus_tag="VC394_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEP5994514.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAYVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            complement(926..1864)
                     /locus_tag="VC394_001040"
     CDS             complement(926..1864)
                     /locus_tag="VC394_001040"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEP5994515.1"
                     /translation="MIEFEKPIITKIDENKDYGRFVIEPLERGYGTTLGNSLRRVLLS
                     SLPGAAVTSIKIDGVLHEFDTIPGVREDVMQIILNVKGLAVKSYVEDEKIIELEVEGP
                     AEVTAGDILTDSDIELVNPDHYLFTIAEGHSLRATMTVAKKRGYVPAEGNKKDDAPVG
                     TLAVDSIYTPVKKVNYQVEPARVGSNDGFDKLTIEIMTNGTIIPEDALGLSARVLIEH
                     LNLFTDLTEVAKATEVMKETEKVNDEKVLDRTIEELDLSVRSYNCLKRAGINTVFDLT
                     EKSEPEMMKVRNLGRKSLEEVKVKLADLGLGLKNDK"
     gene            complement(1910..2293)
                     /gene="rpsK"
                     /locus_tag="VC394_001041"
     CDS             complement(1910..2293)
                     /gene="rpsK"
                     /locus_tag="VC394_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEP5994516.1"
                     /translation="MAKPTRKRRVKKNIESGVAHIHATFNNTIVMITDVHGNALAWSS
                     AGALGFKGSRKSTPFAAQMAAEAAAKSAQEHGLKTVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            complement(2311..2676)
                     /gene="rpsM"
                     /locus_tag="VC394_001042"
     CDS             complement(2311..2676)
                     /gene="rpsM"
                     /locus_tag="VC394_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEP5994517.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDIR
                     VKDLTSDQEDAIRREVDAIKVEGDLRREVNMNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            complement(2694..2810)
                     /gene="rpmJ"
                     /locus_tag="VC394_001043"
     CDS             complement(2694..2810)
                     /gene="rpmJ"
                     /locus_tag="VC394_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEP5994518.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPTNPKHKQRQG"
     gene            complement(2836..3054)
                     /gene="infA"
                     /locus_tag="VC394_001044"
     CDS             complement(2836..3054)
                     /gene="infA"
                     /locus_tag="VC394_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEP5994519.1"
                     /translation="MAKEDVIEIEGKVVETMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILVGDRVTVEMSPYDLTRGRITYRFK"
     gene            complement(3172..3810)
                     /locus_tag="VC394_001045"
     CDS             complement(3172..3810)
                     /locus_tag="VC394_001045"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEP5994520.1"
                     /translation="MNLLIIGLPGAGKGTQAAKIVEEFGVAHISTGDMFRAAMANQTE
                     MGRLAKSYIDKGELVPDEVTNGIVKERLAEDDIAEKGFLLDGYPRTIEQAHALDATLE
                     ELGLRLDGVINIKVDPSCLVERLSGRIINRKTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNMAQGEPILEHYRKLGLVTDIEGNQEITDVFADVEKALLELK"
     gene            complement(3960..5264)
                     /gene="secY"
                     /locus_tag="VC394_001046"
     CDS             complement(3960..5264)
                     /gene="secY"
                     /locus_tag="VC394_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEP5994521.1"
                     /translation="MFLKILKDALKIKTVRNKIFFTIFIILVFRIGTHITVPGVNAKS
                     LEQLSELPFLNMLNLVSGNAMRNFSVFSMGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYISLVLAFAQSIGITAGFNTLSNVALVKTPDIKTYLLIGALLTTG
                     SVIVTWLGEQITDKGFGNGVSMIIFAGIISSIPSAIATIREDYFVNVKASDLHSSYLI
                     VGILIIAVLAIVFFTTYVQQAEYKIPIQYTKLMQGAPTSSYLPLKVNPAGVIPVIFAS
                     SITTIPSTIIPFVQNGRDLPWLNRLQEIFNYQTPVGMIVYALLIILFSFFYTFVQVNP
                     EKTAENLQKNSSYIPSVRPGRETEQFMSALLKKLATVGAIFLAFISLAPIAAQQALNL
                     SSSIALGGTSLLILISTGIEGMKQLEGYLLKRKYVGFMNTAE"
     gene            complement(5281..5721)
                     /gene="rplO"
                     /locus_tag="VC394_001047"
     CDS             complement(5281..5721)
                     /gene="rplO"
                     /locus_tag="VC394_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEP5994522.1"
                     /translation="MKLHELKAAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRIPKRGFTNINTKEYALVNLDQLNVFDDGTEVTPAILKDAGIV
                     RAEKSGVKVLGNGELTKKLTVKAAKFSKSAEAAIIAKGGSIEVI"
     gene            complement(5936..6118)
                     /gene="rpmD"
                     /locus_tag="VC394_001048"
     CDS             complement(5936..6118)
                     /gene="rpmD"
                     /locus_tag="VC394_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002959367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEP5994523.1"
                     /translation="MAQIKITLTKSPIGRKPEQRKTVVALGLGKLNSSVVKEDNAAIR
                     GMVTAISHLVTVEDVK"
     gene            complement(6133..6627)
                     /gene="rpsE"
                     /locus_tag="VC394_001049"
     CDS             complement(6133..6627)
                     /gene="rpsE"
                     /locus_tag="VC394_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEP5994524.1"
                     /translation="MAFKDNAVELEERVVAINRVTKVVKGGRRLRFAALVVVGDGNGR
                     VGFGTGKAQEVPEAIRKAVEAAKKNMIEVPMVGTTIPHEVYTNFGGAKVLLKPAVEGS
                     GVAAGGAVRAVIELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEVAALRGISV
                     SDLA"
     gene            complement(6646..7002)
                     /gene="rplR"
                     /locus_tag="VC394_001050"
     CDS             complement(6646..7002)
                     /gene="rplR"
                     /locus_tag="VC394_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEP5994525.1"
                     /translation="MISKPDKNKIRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKDVSKGTKTEQAVVVGKLVAERAVAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            complement(7107..7643)
                     /gene="rplF"
                     /locus_tag="VC394_001051"
     CDS             complement(7107..7643)
                     /gene="rplF"
                     /locus_tag="VC394_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEP5994526.1"
                     /translation="MSRIGNKVITMPAGVELTNNNNVITVKGPKGELTREFNKNIEIK
                     VEGTEITVVRPNDSKEMKTIHGTTRANLNNMVVGVSEGFKKDLEMKGVGYRAQLQGTK
                     LVLSVGKSHQDEVEAPEGITFTVANPTSISVEGINKEVVGQTAAYIRSLRSPEPYKGK
                     GIRYVGEYVRLKEGKTGK"
     gene            complement(7846..8244)
                     /gene="rpsH"
                     /locus_tag="VC394_001052"
     CDS             complement(7846..8244)
                     /gene="rpsH"
                     /locus_tag="VC394_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEP5994527.1"
                     /translation="MVMTDPIADFLTRIRNANQVKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEVIEDDKQGIIRVFLKYGKNGERVITNLKRISKPGLRVYAKRDDMPKVLNGLG
                     IAIISTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            complement(8395..8580)
                     /locus_tag="VC394_001053"
     CDS             complement(8395..8580)
                     /locus_tag="VC394_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEP5994528.1"
                     /translation="MAKKSMIAKNKRPAKHSTQAYTRCEKCGRPHSVYRKFKLCRVCF
                     RELAYKGQIPGVVKASW"
     gene            complement(8596..9138)
                     /gene="rplE"
                     /locus_tag="VC394_001054"
     CDS             complement(8596..9138)
                     /gene="rplE"
                     /locus_tag="VC394_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEP5994529.1"
                     /translation="MANRLKEKYTNEVIPALTEKFNYTSVMAVPKVEKIVLNMGVGDA
                     VSNAKNLEKAAAELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEISFDDVDKVRGLDIVIVTT
                     ANTDEESRELLKGLGMPFAK"
     gene            complement(9162..9467)
                     /gene="rplX"
                     /locus_tag="VC394_001055"
     CDS             complement(9162..9467)
                     /gene="rplX"
                     /locus_tag="VC394_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEP5994530.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLKALPKVNKVIVEGVGMIKKH
                     QKPNTENPQGAIVEKEAPIHVSNVQVLDKNGVAGRVGYKVVDGKKVRYSKKSGEVLD"
     gene            complement(9546..9914)
                     /gene="rplN"
                     /locus_tag="VC394_001056"
     CDS             complement(9546..9914)
                     /gene="rplN"
                     /locus_tag="VC394_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEP5994531.1"
                     /translation="MIQQETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKTGARRPDGSYIKFDDNAAVIIRDDKTPRGTRIFGPV
                     ARELREGGYMKIVSLAPEVL"
     gene            complement(9939..10199)
                     /gene="rpsQ"
                     /locus_tag="VC394_001057"
     CDS             complement(9939..10199)
                     /gene="rpsQ"
                     /locus_tag="VC394_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEP5994532.1"
                     /translation="MERNQRKTLYGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEKAVII"
     gene            complement(10225..10431)
                     /locus_tag="VC394_001058"
     CDS             complement(10225..10431)
                     /locus_tag="VC394_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEP5994533.1"
                     /translation="MKLQEIKDFVKELRGLSQEELAKKENELKKELFDLRFQAAAGQL
                     EKTARLDEVKKQIARVKTVQSEMK"
     gene            complement(10441..10854)
                     /gene="rplP"
                     /locus_tag="VC394_001059"
     CDS             complement(10441..10854)
                     /gene="rplP"
                     /locus_tag="VC394_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEP5994534.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVSFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            complement(10858..11511)
                     /gene="rpsC"
                     /locus_tag="VC394_001060"
     CDS             complement(10858..11511)
                     /gene="rpsC"
                     /locus_tag="VC394_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEP5994535.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFINK
                     ELADASVSTIEIERAVNKVIVSLHTAKPGMVIGKGGANVDALRGQLNKLTGKQVHINI
                     IEIKQPDLDAHLVGENIARQLEQRVAFRRAQKQAIQRTMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            complement(11524..11868)
                     /gene="rplV"
                     /locus_tag="VC394_001061"
     CDS             complement(11524..11868)
                     /gene="rplV"
                     /locus_tag="VC394_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEP5994536.1"
                     /translation="MAEITSAKAMARTVRVSPRKTRLVLDLIRGKKVADAIAILKFTP
                     NKAARVIEKTLNSAIANAENNFGLEKANLVVSETFANEGPTMKRFRPRAKGSASPINK
                     RTTHVTVVVSEK"
     gene            complement(11884..12162)
                     /gene="rpsS"
                     /locus_tag="VC394_001062"
     CDS             complement(11884..12162)
                     /gene="rpsS"
                     /locus_tag="VC394_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEP5994537.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRR"
     gene            complement(12302..13135)
                     /gene="rplB"
                     /locus_tag="VC394_001063"
     CDS             complement(12302..13135)
                     /gene="rplB"
                     /locus_tag="VC394_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEP5994538.1"
                     /translation="MGIKVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVSLKSKAGRN
                     NNGRITVRHQGGGHKRHYRLIDFKRNKDGVEAVVKTIEYDPNRTANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPDADIKVGNALPLANIPVGTVVHNIELKPGKGGELVRAAGA
                     SAQVLGQEGKYVLVRLQSGEVRMILGTCRATIGTVGNEQQSLVNIGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            complement(13153..13449)
                     /locus_tag="VC394_001064"
     CDS             complement(13153..13449)
                     /locus_tag="VC394_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEP5994539.1"
                     /translation="MNLYDVIKKPVITEKSMIALEAGKYTFEVDTRAHKLLIKQAVEA
                     AFDGVKVASVNTVNVKPKAKRVGRYTGFTSKTKKAIITLTADSKAIELFAAEAE"
     gene            complement(13449..14072)
                     /gene="rplD"
                     /locus_tag="VC394_001065"
     CDS             complement(13449..14072)
                     /gene="rplD"
                     /locus_tag="VC394_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEP5994540.1"
                     /translation="MANVKLFDQTGKEVSSVELNDAIFGIEPNESVVFDVVISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSAKVAEDKFVAVEGLSFAAPKTAEFAKVLSALSIDTKVLVLVEEGN
                     EFAALSARNLPNVTVATAATASVLDIVNADKLLVTKEAISTIEEVLA"
     gene            complement(14096..14722)
                     /gene="rplC"
                     /locus_tag="VC394_001066"
     CDS             complement(14096..14722)
                     /gene="rplC"
                     /locus_tag="VC394_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEP5994541.1"
                     /translation="MTKGILGKKVGMTQIFTESGEFIPVTVIEATPNVVLQVKTVETD
                     GYEAVQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNIEGLEVGAELSVEQFEA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRLAG
                     RMGGNRVTVQNLEIVQVIPEKNVILIKGNVPGAKKSLITIKSAVKAAK"
     gene            complement(14970..15278)
                     /gene="rpsJ"
                     /locus_tag="VC394_001067"
     CDS             complement(14970..15278)
                     /gene="rpsJ"
                     /locus_tag="VC394_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEP5994542.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAEKIVETATRTGATVAGPVPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLVDIINPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            complement(15477..16766)
                     /locus_tag="VC394_001068"
     CDS             complement(15477..16766)
                     /locus_tag="VC394_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEP5994543.1"
                     /translation="MIYNRRKIFSLALPSMIENILQMLMGMVDNYLVAQIGLVAVSGV
                     SIANNIISIYQSLFIALGAAVSSLIARSIGENNQNKQLNYMAGVLQVTLLLSVGLGLL
                     SVVGHHQVLEWLGAEASVTLVGGQYLSIVGGMIVSLGLLTSLGAIVRAQGYPKIPMQV
                     SLLINVLNAIFSALSIYVWGFGLLGVAWATVLSRLVGVFLLCQFIPIKQVAKRLMRPL
                     DKIIFDLSLPAAGERLMMRAGDVLIIGIVVRFGTTALAGNAIGETLTQFNYMPGLAMA
                     TATIILVARQLGGGKVTEIRYIIREAFILSTLMMLVMGALTYLLGPSLLPLFTQNTDA
                     QRSAMVVLLFSLLGAPATAGTLVYTAVWQGLGKAKLPFYATTIGMWVIRIGLGYVIGV
                     VWQYGLIGVWMATVLDNTSRWFILSKQFKKYQEITFH"
     gene            complement(17154..18170)
                     /gene="adhP"
                     /locus_tag="VC394_001069"
     CDS             complement(17154..18170)
                     /gene="adhP"
                     /locus_tag="VC394_001069"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEP5994544.1"
                     /translation="MKAVVVNQASTGVEVVEHDLPNVGHGEALVKVEYCGVCHTDLHV
                     AHGDFGQVPGRILGHEGIGIVEEIGEGVTSLKVGDRVSIAWFFEGCGHCEYCTTGRET
                     LCRSVKNAGYSVDGGMSEYAVVTADYAVKVPEGLDPAQASSITCAGVTTYKAIKEAGA
                     APGQWIVIFGAGGLGNLAVQYAKKVFNAHVVAVDINNDKLELAKEVGADILVNGKEIE
                     DVPGYIQEKTGGAHGVVVTAVSKVAFNQAIDSVRAGGTVVAVGLPSEYMELSIVKTVL
                     DGIKVVGSLVGTRKDLEEAFAFGAEGLVAPVVEKVPVDTAPEVFDEMERGLIQGRKVL
                     DFIS"
     gene            complement(18422..21064)
                     /gene="adhE"
                     /locus_tag="VC394_001070"
                     /gene_synonym="adhC"
     CDS             complement(18422..21064)
                     /gene="adhE"
                     /locus_tag="VC394_001070"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEP5994545.1"
                     /translation="MTEGHNTVETTSVSVTINALVQKGLAALEEMRKLDQEQVDYIVA
                     KASVAALDAHGELAKHAYEETGRGVFEDKATKNLFACEHVVNNMRHQKTVGIIEEDDV
                     TGLTLIAEPVGVICGITPTTNPTSTAIFKSLISLKTRNPIIFAFHPSAQESSAHAARI
                     VRDAAIAAGAPENCVQWVETPSLEATNALMNHDGIATILATGGNAMVKAAYSCGKPAL
                     GVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDDFVAEFKS
                     YHTYFVNKKEKALLEEFCFGAKANSKNCAGAKLNPNIVGKPATWIAEQAGFTVPEGTN
                     ILAAECKEVSENEPLTREKLSPVIAVLKSESREDGVEKARQMVEFNGLGHSAAIHTAD
                     AELAKEFGTRIRAIRVIWNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNAVGDNVSAIN
                     LLNIKKVGRRRNNMQWFKVPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGFLDR
                     IIEQLDLRRNKVVYQIFAEVEPDPDITTVMKGTELMRTFKPDTIIALGGGSPMDAAKV
                     MWLFYEQPEVDFHDLVQKFMDIRKRAFKFPELGKKTKFVAIPTTSGTGSEVTPFAVIS
                     DKANNRKYPIADYSLTPTVAIVDPALVLTVPGFIAADTGMDVLTHATEAYVSQMANDF
                     TDGLALQAIKIVFDNLEKSVKIADFEAREKMHNASTMAGMAFANAFLGISHSMAHKIG
                     AQFHTVHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIAKLLGLPASTP
                     EEAVESYAKAVYDLGCRVGIQMNFKAQGIDENEWKEHSRELAYLAYEDQCSPANPRLP
                     MVDHMQEIIEDAYYGYAERPGRRK"
BASE COUNT         6071 a         4480 c         3815 g         6724 t
ORIGIN      
        1 aattttgttg gaaacggctt aaagtgacag attgtaaaag taaaaaagat attttcacct
       61 ccaaattaaa agaggatttt aaaatactta ttaataaaat attgaatagg ggataaaact
      121 aacaatatag tttgagcatc tgtttgcgat gcaagttttt ttcggacatt taaacgaggg
      181 gattacaccg tttatttgcc catgagaggt ctttaaacac ctgcataagg ttttcttatt
      241 ctgatgacct tattgtctct gagaaaactt tataaacgca tcagaagagg cttctgtgtt
      301 attgttttac tcagcttaaa agcgggagtg tcacctcata atacaacaaa acgcctgctt
      361 ataaaaagtt tctatagctt agataccttt cataaacaag cgtcacatat taacaatatg
      421 atgagtgttc ccgctaggaa aaatcctagc ggtagaccag agctagacta agaataatct
      481 ctcgattact ccatcatcat aacactcaac aaaattgata aaaattaaac taattcaata
      541 attgccattg gagcagcatc tccacggcgt ggttctgttt taagaatacg agtgtatcca
      601 ccgttacgtt ctgcataacg aggtgcaatt tctgagaaaa gtttttgaag agctgtcgtt
      661 gatgtgtatt tatcggtagc ttcatcataa ttttctgatg cgatttcgtt acgaacgtat
      721 gcagctgctt ggcgacgagc atgaagatca ccacgtttac caagagtaat cattttttca
      781 actgttttac ggatttcttt tgcacgtgct tctgtcgtta caattgattc gttgatcaaa
      841 agatctgtcg tcaaatcacg aagcattgct ttacgttgtg agctagtgcg tcctagttta
      901 cggtaagcca ttttgtcctc ctatattatt tatcgttttt tagtccgaga cctaagtcag
      961 caagtttaac cttaacttct tcaaggctct tacgtccaag attacggact ttcatcattt
     1021 caggctcaga tttttctgtt aagtcgaaga cagtgttaat gcctgcacgt tttaaacagt
     1081 tatatgagcg tacggataaa tcaagctcct cgatggtgcg gtcaagtacc ttttcatcgt
     1141 tcactttttc agtttctttc attacttcgg tagcttttgc aacctctgtt aaatcagtaa
     1201 acaggtttaa gtgttcgatc aaaactcgag cagatagacc taaagcatct tcaggaataa
     1261 ttgttccgtt tgtcatgatt tcaatcgtta atttatcaaa gccatcattg ctacctacac
     1321 gggcaggttc aacttgataa ttaacttttt taactggcgt atagattgaa tctactgcca
     1381 atgtacccac aggtgcatca tcttttttgt ttccttctgc tggcacataa ccacgttttt
     1441 tagcaacagt cattgttgca cgtagagaat gtccttcagc gattgtaaaa agataatgat
     1501 ccgggttaac aagttcgata tcactgtcag taagaatatc accagctgtt acctctgctg
     1561 gtccttctac ttcaagttcg ataatctttt cgtcttcgac gtatgatttc acagcaagtc
     1621 ccttaacatt aaggataatt tgcatgacat cttcacgtac acctgggatt gtatcaaatt
     1681 cgtgtagtac tccatcaatt ttaattgatg ttactgctgc acctggaagt gaagacaaga
     1741 gtacacgacg aagagaatta cctagagttg ttccgtagcc acgttcaagt ggttcgatga
     1801 caaatctacc gtaatcttta ttttcatcaa tttttgttat tattggtttt tcaaactcaa
     1861 tcattttttc acccctcgaa acgaaacttg tgtactattt gttatatgat tatacacgac
     1921 gacgttttgg aggacgagca ccattatgtg gcacaggggt cacatcacga attgcagtta
     1981 cttcaagacc tgcagctgct agagcacgaa tagctgattc acgacctgaa ccaggacctt
     2041 ttacagtcac ttcaacagtt tttagtccgt gttcttgtgc agattttgca gcagcttctg
     2101 cagccatttg agcagcaaat ggagtagatt tacgagaacc tttgaaacca agagcaccag
     2161 ctgatgacca tgcaagagca ttaccatgca catctgtaat cataacaata gtgttattaa
     2221 atgtagcgtg aatgtgtgca acaccagatt cgatgttctt tttcacacga cgtttacgtg
     2281 ttggtttagc caattgtttt acctcctatt ttattttttc ttacctgcaa tcgcaacagc
     2341 tttcccttta cgagtgcgag cattgttttt agtattttgt ccacggacag gaagtccacg
     2401 acgatgacgg attccacggt atgaaccgat ttccatcaaa cgtttaatgt tcatgtttac
     2461 ttcacgtcga aggtcacctt caactttgat tgcatccact tcacgacgga tagcatcttc
     2521 ttgatctgat gttaaatctt taacacggat atcttcagaa atacctgcag ctgctaagat
     2581 ttttttagat gttgcaagac caattccgta aacataagta agtgaaatta ctacgcgttt
     2641 atcatttgga atatcaactc cagcaatacg agccattttt tctcctttct attttatcct
     2701 tgacgttgtt tgtgttttgg atttgtcgga caaattacca taacacgacc attacggcga
     2761 ataactttac agtattcgca aattggttta accgatggtc ttaccttcat gttttaatcc
     2821 ctccaattat ttcgattatt taaagcggta tgtgatgcgt ccacgtgtca agtcataagg
     2881 actcatttct actgtaacac ggtcacctac taaaatacga atgtaatttt tgcggatttt
     2941 tcctgataca gttgctagaa tttgatgtcc attttccaat tcaacagtaa acattgcatt
     3001 tggcatcgtt tctactactt tgccttcaat ttcaatcacg tcttcttttg ccacgcaaaa
     3061 gtacccccta aatttcgatt tgatgtcctc tgagcacaga ggtaacaatt ataagtcaga
     3121 ctaaactagt atatcattac taatctactt atgcaagtag attagtataa tttatttgag
     3181 ttctagcaac gctttttcaa catctgcaaa aacgtctgtt atttcttgat taccttcaat
     3241 atctgtaaca agaccaagct tacgatagtg ttcaagaata ggttctcctt gagccatatt
     3301 gacgtccaaa cgacgtttaa cagtttcagg cttatcatct tcacgttgat agtaatcttc
     3361 ttctttataa tctactggtg ggttgaacac tttatggaac gtttcaccag ttttacgatt
     3421 gataatacga ccactcaaac gctctacaag acatgatgga tccactttaa tattaataac
     3481 accatctaag cggagtccta gttcttcaag tgttgcatct aaggcgtgtg cttgttcaat
     3541 agtacgtgga tacccatcaa gcaaaaaacc tttttctgcg atatcatcct cagctaagcg
     3601 ctcttttaca atcccgtttg tgacttcatc aggaaccaat tcacctttat caatataact
     3661 tttagctaaa cgtcccattt cggtttgatt agccattgcg gcgcggaaca tatcccctgt
     3721 tgagatgtga gcaacaccaa attcttcaac aatcttagct gcttgggtcc ctttaccagc
     3781 tcccggcaaa cctatgatta aaagattcat cttttcctcc tcttttaaaa taaatacata
     3841 gtaggaaatt attttccttg gtttttgttt tcaaatggca acaagaaatc gtttgatgat
     3901 aaccaagtct tattgtcatt taaaaacaaa atagaaggtt gacaatgtca acctgtattc
     3961 tattctgctg tattcataaa tccgacatat tttctcttta gaagataccc ctcaagctgt
     4021 ttcatacctt cgataccagt tgaaatcaaa atgagcaaac tagttccacc taaagcaata
     4081 ctagaagaaa ggttgagagc ttgttgtgct gctattggcg ctaaagagat aaatgctaag
     4141 aagatagctc ctacggttgc tagttttttt agcaatgcgg acataaattg ttctgtctca
     4201 cgtccagggc gaacacttgg tatatatgag gaattcttct gaagattttc tgctgtcttc
     4261 tcaggattaa cttgtacaaa ggtatagaag aatgagaaca atataatcaa caaggcgtaa
     4321 actatcattc cgactggagt ttgataatta aaaatttctt gcaaacggtt taaccacggt
     4381 aaatctctac cattttggac aaaaggaata atcgtacttg ggatagttgt aatcgagcta
     4441 gcaaagataa cgggaataac gccggctgga tttactttta atggaagata tgaacttgta
     4501 ggtgcacctt gcattagctt tgtgtattgg attggaattt tatattccgc ttgttggaca
     4561 tatgttgtaa agaaaacaat agcaagaaca gctatgatta aaatcccaac aatgagataa
     4621 gacgagtgta aatcacttgc cttaacattt acaaaatagt cttcacgaat tgttgcaatt
     4681 gcacttggaa tagacgaaat aatacccgca aagataatca ttgatacgcc attaccaaat
     4741 cctttatcgg tgatttgttc tccaagccag gtaacaataa cgctacctgt tgtcagtaat
     4801 gccccgatca gtaaatatgt tttgatgtct ggtgtcttaa caagtgcaac gttcgacaaa
     4861 gtattaaacc ctgcagtgat accaatggat tgggcaaaag ccaaaacaag tgaaatatag
     4921 cgcgtcgctt gatttaattt acgacgtcca acctcacctt gtttgcccca ctcaacaaac
     4981 ttaggtaaaa tatccatctg caataactga acaacgatag aagcagtaat atatgggcta
     5041 acccccattg aaaatactga gaaatttctc atcgcattac cactaactaa gtttaacata
     5101 tttaagaaag ggagttcact taattgctct aagctcttgg catttacacc aggtacggta
     5161 atatgtgtcc caattcggaa tacaagaatg ataaatatag taaagaaaat tttattccgt
     5221 acagtcttta tctttaacgc gtcttttagt atttttaaga acataatgag tatcccctca
     5281 ttagatgact tcgattgaac cacctttagc aataatagct gcttcagcag attttgagaa
     5341 cttagctgct tttacagtca attttttagt caattcaccg ttaccaagca ctttaacgcc
     5401 tgatttttca gctcggacaa taccagcatc ttttaaaata gccggagtca cttctgtacc
     5461 atcatcaaaa acattcagtt gatcaagatt cacaagtgca tattctttag tattaatatt
     5521 tgtaaagcca cgttttggaa tacgacggaa caatggtgtt tgtccacctt caaaacctaa
     5581 acgtacaccg ccaccgctac gagctttttg acctttttga ccgcgaccag atgttttacc
     5641 attacctgat gatgtcccac gaccaacacg gttacgtact ttacgtgagc cttcagcagc
     5701 ttttaattca tgaagtttca ttttttctcc tttttttgta aaatgctagc gcctctatga
     5761 gggaaaagga ctccctatag aaactcgcct atacattaat gatacttaag ttatccaaaa
     5821 taacttaata cagatacgca ctaaccgact actgtttgtt gccaaaaaag caaactacaa
     5881 gagtcggttt tgtgctcgtt tttaattatg aaatcagaga tttcacagtt ataacttatt
     5941 taacatcttc aacggtaacc aagtgagaaa tagctgtcac cataccacgg atagcagcat
     6001 tatcttcttt gactactgaa gagtttaatt taccaagtcc aagagcaaca acagttttac
     6061 gttgttctgg cttacgtccg ataggagact tagtcaaagt aattttaatt tgagccatga
     6121 tgatcccctt tcttatgcta agtcagaaac tgagatgcca cgaagtgcgg caacttcttc
     6181 tgcacgttta agttgtttca atccttcaac agttgcacga acaatgttga ttggagtgtt
     6241 tgaaccaaga gattttgaag taatatcagc aacacctgct aattcgatga cggcacgaac
     6301 tgcaccacca gcagcaactc cagaaccttc tacagctggt ttcaataata ctttagctcc
     6361 accgaagtta gtgtaaactt cgtgagggat tgttgtacca accattggta cttcgatcat
     6421 gtttttctta gcagcttcaa ctgctttacg gatagcttct ggaacttctt gagctttacc
     6481 agtaccaaat ccaacacgac catttccatc accaacaact acaagagctg caaagcgaag
     6541 acgacgtcca cctttaacaa cttttgtaac gcggttaatc gcaacaacgc gttcttcaag
     6601 ttcaactgca ttatctttaa atgccattat tttgtgtcct ccctattaga atttcaatcc
     6661 gttttcacga gctgcatcag ccaaggcttt aacacgtccg tgatagagat atccaccgcg
     6721 gtcaaacacc acttcagaaa tacctttagc cactgcacgt tcagcaacaa gtttgccgac
     6781 tacaacggct tgttctgttt ttgttccttt agaaacgtct ttatcaagag ttgatgcact
     6841 tgcgagcgtt acacccgcta cgtcatcaat aacttgagcg tagatgcctg tattagaacg
     6901 gaatacgttc aaacgtgggc gatcagcagt tccagagagt ttaccgcgga cgcgacggtg
     6961 gcgtttttgg cggattttgt ttttatctgg tttcgaaatc acaattttca cctcttaatt
     7021 ttaatcaatt tgtctcattg acaagttgtc gattgaactt atcgagcatg ttaaggtgca
     7081 atacttccca tacccgacta agaacattat ttacctgttt taccttcttt aaggcgtacg
     7141 tattcaccaa cgtaacggat ccctttgcct ttgtaaggct ctggtgaacg caagctacgg
     7201 atgtaagcag ctgtttgacc aacaacttct ttgttgattc cttcaactga gattgaagtt
     7261 gggttagcaa cagtgaaagt aattccttct ggagcttcaa cttcgtcttg gtgagactta
     7321 cctactgaaa ggacaagttt agtaccttga agttgagcac ggtaaccgac acccttcatt
     7381 tcaagatctt ttttgaaacc ttcagaaaca cctacaacca tgttattcaa gttagcacgg
     7441 gttgtcccat ggattgtttt catttctttt gagtcgttag gacgtacaac tgtgatttca
     7501 gtcccttcaa ctttgatttc aatatttttg ttgaactcac gagtgagttc gcctttaggg
     7561 cctttaacag taataacatt gttgttattt gttaattcaa cgcctgcagg catagtaatt
     7621 actttattac caatacgtga catgtttatt ttctcctgtt aaattgtcaa gccgcatagc
     7681 gactagtttt cacgggggtt tgatagaatg atatacttca atttatattt caaaattgaa
     7741 cacgagctgt ggtctgtgtg aaaaagataa atcttccttg agtgtaaaca cgctacgtca
     7801 gatttcctat tttcactttg tccacgacgc tcttagtatc ttaaattacc aaacgtaagc
     7861 gatcacttct ccaccaacat tcttttggcg agcttctttg tcagtcaaaa gaccttctga
     7921 tgttgaaatg attgcaattc caagtccgtt aagaacttta ggcatgtcat cacgttttgc
     7981 gtaaacgcga agacctggtt ttgaaatacg ttttaagtta gtgataacac gctcaccatt
     8041 ttttccgtat tttaagaaca cacgaatgat gccttgttta tcatcttcga taacttcaac
     8101 attttttaca aaaccttcac gtttaagaat ctcagcaatc ccttttttaa tgtttgaagc
     8161 aggtacttct aacacttcgt gttttacttg attagcatta cggatacgtg ttaaaaagtc
     8221 tgcaattggg tcagtcataa ccatttttta tattctcctc ttactagcag tttgatgcta
     8281 tcacttacta gttaatgttg tccttaaagg actttggtat cagcttatgc tcacaccgtt
     8341 ttaattaagc acaagactta atctgaagaa caaattaagt tttgtgtcat atttttacca
     8401 agaagctttg acaacacctg gaatttgacc tttgtaagcc aattcacgga agcaaacacg
     8461 acaaagttta aacttgcggt aaactgaatg tggacgtcca catttttcac agcgagtata
     8521 agcttgtgta gagtgttttg caggacgttt gttcttagca atcatagatt ttttagccaa
     8581 tttatttacc ccctattatt ttgcaaaagg cattccaagg cccttaagca attcacgtga
     8641 ttcttcgtca gtatttgcag tagtgacgat tacaatatca agaccacgta ctttatcaac
     8701 atcatcaaag ctgatttctg ggaagataag ttgttctttc acaccgagag tgtagtttcc
     8761 acgtccatca aacgattttg tcggaacgcc atggaagtca cgtacacgag gaagtgaaac
     8821 gctaaccaat ttgtctagga attcgtacat acgttcgcca cggagggtaa cttttgcacc
     8881 gatcgctaca ccttcacgaa gacggaagcc agcgattgat ttcttagctt tagtaataag
     8941 tggtttttga cctgagataa gtgctaattc agctgcagct ttctctaggt tttttgcgtt
     9001 ggatacagca tcaccaacac ccatattgag aacaattttc tcaacttttg gcacagccat
     9061 aactgatgtg taattgaatt tctctgtcaa cgcaggaatt acttcgttag tatatttttc
     9121 ttttaaacga tttgccatta tgcttctcct ttccttcgtg attaatcaag cacttcgcct
     9181 gattttttgc tgtaacgtac ttttttaccg tcaacaactt tataaccaac acgtccagct
     9241 acaccatttt tatctaatac ttgaacatta gagacatgga taggtgcttc tttttcgacg
     9301 atagctcctt gaggattttc agtgttaggt ttttggtgtt ttttaatcat tccaacacct
     9361 tcaacaataa ctttgttaac ttttgggagt gctttaagaa ctacagcttc agtgcctttg
     9421 tccttgccag caattacgcg aactttgtcg ccttttttta caaacattct ggtttctcct
     9481 attatttctt acgccccgat gggcacccta ggaaaaccta ggggactacg tttgttaatc
     9541 tgagattaaa gtacttctgg tgcaagtgat acgatcttca tgtagccacc ctcacgcaat
     9601 tcgcgtgcaa ctgggccaaa gatacgagtt ccgcgaggag ttttatcatc acggatgatt
     9661 acagcagcat tgtcgtcaaa tttgatgtat gaaccgtctg gacggcgtgc accagttttt
     9721 gtacgaacga taacagcttt aaccacatca ccttttttaa ctgctccacc aggagtagct
     9781 tgttttacag aagcaacgat tacgtcaccg atgttagcga atttacgtcc tgaaccacca
     9841 agtactttga tagtcaagat ctcacgagca ccgctattat cagcaacttt caagcgagtt
     9901 tcttgttgaa tcatttcaat tttctccttt tagtttgatt agataataac agctttttcc
     9961 actacttcca caagacggaa acgttttgta gctgaaagtg ggcgagtttc catgatacga
    10021 acgatatcgc cttctttagc aacgttgttt tcatcatgtg ctttatattt tttagaatag
    10081 ttgatacgtt taccatagac tgggtggtta cgtttagttt caactacaac tgtgattgtt
    10141 ttgtccatct tgtcagacac tacgcgtccg taaagagttt tacgttgatt acgttccatt
    10201 tagaatttct cctttcccaa tctattattt catttctgat tgcacagttt taacacgtgc
    10261 aatttgtttc ttaacttcgt caaggcgagc agtcttttca agttgacctg ctgcagcttg
    10321 gaaacgaaga tcaaacaatt cttttttgag ttcgttttct ttcttagcaa gttcttcttg
    10381 agacaatcca cgaagctctt taacaaaatc tttaatttct tgaagtttca tgtcttctcc
    10441 ttattctgct tcacgtttta cgaatttgca cttaactggt aatttgtggc tagcaagacg
    10501 taatgcttcg cgagcaattt cttcagaaac accagcaatt tcaaacatca ctttaccacg
    10561 tttaactggt gctacccaac cttcaggtgc ccctttacca gaacccatac gtacaccgat
    10621 agcttttgca gtgtatgatt tgtgtgggaa gattttaatc caaactttac caccacgttt
    10681 catgtaacga gtcatcgcaa tacgggcagc ttcgatttga cggtttgtaa tccagtggct
    10741 agttgtggct tgaagtccgt attcaccgaa tgaaacttct ttaccacctt tagcctcacc
    10801 gcgcattttt ccacggaatt cgcgacggtg tttaacacgt ttaggtacta acatttgtta
    10861 tttgcctcct ttagtgtttt tacgagctgg aagaacttct ccacggtaaa tccaaacttt
    10921 aacgccaagt ttaccataag ttgtgtcagc ttcttcccaa gcatagtcga tatcagcgcg
    10981 aagcgtgtga agtggaacag ttccttctga ataaccttca gcacgagcga tatcagcacc
    11041 gttcaaacga ccagaaactt gagttttaat ccctttagct cctgcacgca ttgtacgttg
    11101 gattgcttgt ttttgagcac gacggaaagc aacacgttgc tcaagttgac gagcaatgtt
    11161 ttcaccaaca aggtgagcat ctaaatcagg ttgtttgatt tcgatgatgt tgatgtgtac
    11221 ttgttttcca gttaatttgt taagttgacc acgaagagcg tcaacgtttg cgccaccttt
    11281 accgataacc atacctggtt tagcagtgtg aagtgaaaca ataactttgt ttactgcacg
    11341 ttcaatttca atagttgaaa ctgatgcgtc agctaattct ttattaatga atttacggat
    11401 tgcaagatct tcatgaaggt aatccgcgta ttctttttca gcataccatt tcgcatccca
    11461 gtcacggatg attccgacac gcataccaat tggatgtact ttttgaccca cgattttacc
    11521 tccttatttt tctgatacaa ctacagttac gtgagttgta cgtttgttga ttggtgaagc
    11581 tgaacctttc gcacgtggac ggaaacgttt cattgttggt ccttcgttag cgaatgtttc
    11641 agatactacc aagtttgctt tttccaaacc aaagttgttt tctgcgttag caattgctga
    11701 gttaagagtt ttctcaataa cacgagctgc tttgtttgga gtgaatttta agattgcgat
    11761 tgcgtcagca actttcttac cacggataag atcaagtact aaacgtgttt tacgaggtga
    11821 aacacgcact gtacgagcca ttgctttagc tgaagtaatt tctgccattg tgtcctcccc
    11881 ctattaacga cgtgttttct tgtcgtcagc tgcgtgacct ttgtaagtac gcgttggtgc
    11941 aaattcacca agtttgtgac ctaccatgtc ttcttggatg taaacaggta catgtttacg
    12001 tccgtcataa actgcgattg tatatccgat gaaacttggg aaaatcgttg aacgacgtga
    12061 ccaagttttg attacttttt tcttttcgtc gtttgcttga gcttcaactt ttttcatcaa
    12121 atgctcatcg acgaaaggtc cttttttaag actacgtccc attttgtagt tttctccttt
    12181 aaataatgta ccacagcggc ttgagttcaa gagcaagact ccaaaactct accgagttgg
    12241 cggaataaat agcaaagcta ttgtaacgct atttcagcaa gttttaagca acttactaag
    12301 attatttttc gttacgacga cgaacgataa gtttgtctga tttagctttc ttgttacgag
    12361 ttttaagacc aagcgctggt ttaccccatg gagttgatgg cgctttacgt ccaactggtg
    12421 ctttaccttc accaccaccg tgtgggtgat cgttagggtt cattacagaa ccacgaactg
    12481 ttgggcggat acctttccaa cggctacgtc ctgccttacc aatgttaaca agtgattgtt
    12541 gttcgttacc aacagtaccg atagttgcac ggcatgtacc aagaatcata cgaacttcgc
    12601 ctgattgaag acgaacaaga acgtatttac cttcttgacc aagtacttga gcagaagctc
    12661 cagctgcacg aacaagttct ccacctttac caggtttcaa ctcaatattg tgaacaactg
    12721 taccgacagg aatgtttgct aatggaagtg cgttaccaac tttgatatct gcatctggac
    12781 cagaaacaat acgttgacct acttcaagac ctttaggtgc aatgatgtaa gctttcacac
    12841 cgtcagtgta atgtacaagt gcgatgtttg cagtacggtt tggatcatat tcgattgttt
    12901 taacaactgc ttcaacgcca tctttgttac gtttgaagtc gatcaaacgg taatgacgtt
    12961 tgtgtccacc accttggtga cgaactgtga tgcgaccatt gttgttacga ccagctttgc
    13021 tcttaagaga aacaagcaat gatttctcag gcgtgcttgt tgtgatttcc gcgaaatcca
    13081 aagaagtcat gttacgacgg ccatttgtcg ttggtttata aactttaata cccacgttat
    13141 ttcctcctta gattattcag cttcagctgc gaacaactcg attgctttag aatcagctgt
    13201 aagagtgatg atagcttttt tagtttttga agtgaaacct gtgtaacgac caacgcgttt
    13261 tgcttttggt ttaacgttaa cagtgtttac acttgcaact ttaactccgt caaaagcagc
    13321 ttcaacagct tgtttgatca aaagtttgtg tgcacgagta tcaacttcga aagtgtattt
    13381 gcctgcttca agagcaatca ttgacttctc agtgataact ggttttttga ttacgtcgta
    13441 caaattcatt atgcaagaac ctcctcaatt gtagagattg cttctttagt aacaagaagt
    13501 ttgtctgcgt tcacgatatc aagaacactt gcagttgctg cagttgcaac agttacgttt
    13561 ggaaggttac gtgcagaaag tgctgcaaat tcatttcctt cttcaacaag aacaagtact
    13621 tttgtatcaa tgctaagagc tgaaagcact tttgcaaatt cagcagtttt tggtgctgca
    13681 aatgaaaggc cttctacagc tacaaattta tcttcagcaa cttttgctga gtaaactgat
    13741 ttcaacgcaa ggcgacgaac tttttgtgga agtttgtatc cgtatgaacg gggagttggt
    13801 ccaaagacaa caccaccacc acgccattgt ggtgagcgga tagaaccttg acgagcgcgt
    13861 ccagttcctt tttgacgcca tggtttacgt ccaccacctg atactgctga acggttttta
    13921 accgcatgag taccttggcg aaggctagcg cgttggctaa ttacaacatc aaaaacaact
    13981 gattcgtttg gttcgatacc gaagatagcg tcgtttaatt caactgagct aacttcttta
    14041 ccagtttggt caaatagttt tacgtttgcc attttaactg ttttctcctt tcttattatt
    14101 tagcagcttt aactgctgac ttgatagtga taagagattt cttagcacct ggtacgttac
    14161 ctttaataag gataacgttc ttttctggga taacttgtac aatttcaagg ttttgaactg
    14221 ttacacggtt accacccata cgtcctgcca agcgtttgtt tttgaaaacg cggttaggcg
    14281 caacaggtcc catagaacct gggcgacgat ggtaacgaga accgtgagcc ataggaccac
    14341 gtgattgacc atggcgtttg ataacacctt ggaaaccttt accttttgat gtccctgtga
    14401 cgtcaacaac atcaccagct tcaaattgtt ctacagataa ttctgcacca acttctaagc
    14461 cttcaatgtt tttgaattca cgaatgaagc gcttaggagc tgtgtttgct tttgcaacat
    14521 ggcctttggc aggtttgtta ctcaagactt cacgtttgtc atcaaaacca acctgaactg
    14581 cttcataacc gtctgtttca acagttttaa cttgaagcac aacgtttgga gttgcttcaa
    14641 tgacagtaac agggatgaat tcgcctgatt cagtgaagat ttgagtcatt cccacttttt
    14701 tccctaagat tccttttgtc atgagaaaat atttcctttt cttaataata tttcaaaaag
    14761 ttttttatga gcgttttttg tgctcaaaaa agttttttac gagcgttttt tgtgctcata
    14821 gcaagataca aagagcgtca aactcaaaat aaaaatagaa aatctaacac aaaaacttct
    14881 gtttcgagga agatttatct ttttcatgta gagtttagct cgtgttcaag ttccatcaaa
    14941 cactaactgt acttgcaaaa tctcaccgat taaagtttga tttctacgtt gacaccactt
    15001 ggaagatcaa gtttcatcaa agcatcaaca gttttttgtg ttggattgat gatgtctacc
    15061 aaacgtttgt gtgtacgcat ttcaaattgt tcgcgagaat ctttgtattt gtgagtcgca
    15121 cgaataattg tgtaaagact gcgttcagtt ggaagtggaa ctggtccagc aactgtagca
    15181 cctgtacgtg ttgcagtttc aacgattttt tctgccgctg tatcaagtgt acggtgttcg
    15241 tacgctttca aacggatacg gatttttttg tttgccatct tttctcctct cgtctattta
    15301 cgataatagg ctagctcctc aagaaaaccg acacctgttg cgtggcaatg caaccgagcg
    15361 tgtcgcaacc tcttgtatca ttgctaaagc tgtattttta cagcaccaca atataataac
    15421 agaatcattt agtcattgca agctttttag ggcatttttt caaaaaaact accttcctaa
    15481 tgaaaggtaa tttcttggta ctttttaaat tgtttagaga gaataaacca ccgactggta
    15541 ttatctaaca ctgtcgccat ccagacccct atgagtccat attgccaaac cacaccaatc
    15601 acataaccaa ggccaatgcg aatcacccac attcctatgg ttgttgcata gaagggaagc
    15661 tttgcttttc ccaaaccctg ccagacggct gtataaacta aagtgcctgc cgttgcggga
    15721 gctcctagta aagaaaaaag taaaacaacc atggcggacc tttgagcatc agtattctga
    15781 gtaaatagcg gcaagagact aggacctagc agataggtca aggctcccat aaccagcatc
    15841 atgagggtgg ataaaataaa agcttctcta ataatatacc ttatctcagt caccttaccg
    15901 ccccccagtt gcctggccac tagaataatt gttgccgttg ccatggctaa gccaggcatg
    15961 taattaaact gagtcaaggt ttctccaata gcattacctg ccaaggccgt tgtcccaaaa
    16021 cgaacaacaa tgccaataat taggacatct cctgctctca tcatcaaacg ttcaccagca
    16081 gcaggtagtg ataaatcaaa aataattttg tccaaaggtc tcatcaatcg tttcgcaacc
    16141 tgtttaatag gaataaactg acaaagtaaa aacacaccca ctaaacgaga taaaaccgtc
    16201 gcccaagcaa caccaagaag accaaatccc catacataga tagacaaggc agaaaaaatg
    16261 gcattgagta cgtttattaa taagctcact tgcataggaa tcttaggata cccttgagct
    16321 cgaacaatag ctcctaagct agttaaaagt cctagactaa caatcatacc gccgacaatg
    16381 gatagatatt gccctccaac caatgttaca gaagcttcag ctcctagcca ttctaacacc
    16441 tgatgatgtc caacaactga taacaaccct aagccgaccg aaaggaggag tgttacttgc
    16501 aagacacctg ccatatagtt taattgtttg ttctgattat tttctccaat acttcgagca
    16561 atcagactag atacagcggc tcccagcgcg ataaagaggg actgataaat actgataatg
    16621 ttgttagcta tggagacacc agagactgct acaagtccaa tttgagccac caggtaatta
    16681 tcaaccattc ccattaacat ctgtaaaata ttttctatca tagaaggcag agctaaagag
    16741 aaaatttttc ttctattata tatcatttat gtcccctaat aaaaaattct tgatcaaacg
    16801 atttttttaa acacagtcac tggactcatt cagttatttt gttccattaa tgaattcata
    16861 aagtctacct taatagcaga gtcccaagat acacgacact tagtgttaca gtaacaattg
    16921 accacatgcc aatccttaac atttacctaa cgtttataac taagaagacg aagaacaaaa
    16981 ggactccaaa cgaatttttt ctattatata atatattctt catttagcaa ctttcaatgt
    17041 atcaaaatag cactctcgag tcaaaaatgt tttctgatac tcgagagtgc tattttattt
    17101 ggaatcaagt ttaagcaact taggcttatc tcatatttaa tacatttgta tgtttagctg
    17161 atgaaatcta agaccttgcg cccttggatt aaaccacgtt ccatttcatc aaagacttct
    17221 ggagcagtgt caactggaac tttttcgaca accggagcaa ccagaccttc tgcaccaaag
    17281 gcaaaggctt cttccaagtc tttacgtgtc cctacaagtg atccgaccac tttgatacca
    17341 tctaaaacag tttttacgat agacagttcc atgtattctg atggaagacc tacggctaca
    17401 actgttccac cggcacggac actatcaatt gcttggttaa aagcaacttt agaaacagcc
    17461 gttaccacaa caccatgtgc tccaccggtt ttttcttgaa tatagcctgg aacatcttca
    17521 atttctttgc cgttaactaa aatatctgcg ccaacttctt tagctaactc aagtttatca
    17581 ttattaatgt ccactgcgac aacatgtgca ttaaagactt ttttagcgta ttgaacagcc
    17641 agatttccaa ggccacctgc cccaaagata acaatccatt ggcctggagc agcacctgcc
    17701 tctttaatag ctttataagt tgttacacca gcacaagtaa tggaagaagc ttgagccggg
    17761 tctaaacctt ctggaacttt aactgcataa tcagctgtta ctactgcata ttcactcatt
    17821 cctccatcaa cactgtatcc agcattttta acactgcgac aaagtgtctc acgtcctgta
    17881 gtacagtatt cgcaatgacc acatccttca aagaaccatg caatagagac acgatcacca
    17941 acctttaagg atgttacgcc ttctccaatt tcttctacta taccaatacc ttcgtgcccc
    18001 aaaatacgtc ctggtacctg accaaaatcg ccatgtgcaa cgtgtaaatc agtatgacaa
    18061 acgccacagt attctacttt aacaagcgct tcaccatgac caacatttgg aagatcatgc
    18121 tcaacaactt cgacacctgt actagcttga ttaacaacta cggctttcat atcagtcctc
    18181 catctaataa cgacttattt gtaatcggtt acaaataaat tgtacatctt ttcacaatta
    18241 aagtcaacag aaacctgaaa agtttctgaa acttaactgg ttaatcttat agctgtttgc
    18301 ctatctgcaa aatacttaac tacattttct gacactggat ataaatgata tatttagaaa
    18361 ataaaatgat ttcttatgac tttaatcata agaaatcatt tcctggtatc aacattaaaa
    18421 atcacttacg tcgtcctgga cgttcagcat aaccatagta agcatcttca ataatttctt
    18481 gcatgtgatc aaccattgga agacgtgggt tagcaggtga acattggtct tcataagcaa
    18541 ggtaagccaa ttcacgagaa tgttctttcc attcattttc gtcgatacct tgagctttga
    18601 agttcatttg aataccaact cggcaaccca gatcataaac agctttcgca taagattcca
    18661 ctgcttcttc tggtgtagaa gctggaagcc ctaacaattt cgcaatgtct tggtattttt
    18721 catccgcacg gtagtaatta tacttaggcc atgtcgccgt tttagctgga cgagttccat
    18781 tatagcggat aacatacggc aaaagaattg catttgtacg tccgtgtacg gtatggaatt
    18841 gtgcgccaat tttatgcgcc attgagtgag aaatacctaa gaatgcattg gcgaaagcca
    18901 tacctgccat agttgacgca ttgtgcatct tttcacgtgc ttcaaaatcg gctattttaa
    18961 ctgatttttc aagattatca aaaacaattt taatggcttg aagagctaaa ccatccgtaa
    19021 agtcattggc catttgtgac acgtaagctt cggttgcgtg tgtcaaaacg tccataccag
    19081 tatcagcagc aataaagcct ggaacagtca ataccaaagc tgggtcaaca atggctacag
    19141 ttggtgttaa tgagtaatca gcgattggat atttacgatt atttgctttg tcagagataa
    19201 cggcaaatgg tgtcacctct gaacctgttc cagaagttgt tgggatagca acaaattttg
    19261 ttttcttacc aagttctggg aatttgaacg cacgtttgcg gatatccatg aatttttgaa
    19321 cgaggtcatg gaaatcaact tctggttgct cgtagaagag ccacataact ttggctgcat
    19381 ccataggaga tcccccgcca agggcaataa tggtgtctgg tttgaaggta cgcattaatt
    19441 cagtaccttt cataacagtt gtaatgtctg gatctggttc tacttcagca aagatctgat
    19501 aaacaacttt attgcgacga agatcaagtt gttcaatgat acgatccaag aaaccaagct
    19561 caaccatagc gtgatctgta acaatcatga cacgttcaac gtcacgacat ttttgcaaat
    19621 attgaattga atcacgttcg aagtatgttt ttgaaggaac tttaaaccat tgcatattat
    19681 ttctacgtct tcctactttt ttgatgttca agagatttat agcactaaca ttatcaccaa
    19741 ctgcgttacg tccatacgaa ccacaaccaa gagtcaatga tggcaagaaa gcattgtaaa
    19801 catccccgat accaccaaat gtagaaggtg aattccagat gacgcggata gcacggattc
    19861 tagttccaaa ttcttttgcc aactctgcat ctgctgtatg aatggcagct gagtgaccga
    19921 gtccattaaa ttcaaccatt tgacgagctt tttcaacacc gtcttcacgt gattcagatt
    19981 tcaaaacagc aatgactgga gataattttt cacgtgttaa aggctcattt tctgaaactt
    20041 ctttacattc tgcagcaaga atattggttc cttctggaac agtaaatcca gcttgttctg
    20101 caatccaagt agctggtttt cctacaatat ttggatttaa ttttgcacca gcacagtttt
    20161 tgctatttgc tttggcacca aaacagaatt cttcaagaag ggctttttct tttttattta
    20221 caaaataagt atggtaagat ttgaattctg ctacaaaatc gtcgtagatt tctttatcga
    20281 tgataacagc ttgttcagag gcacagacca taccattatc aaatgattta gacatcacaa
    20341 tatcatgagc ggcttgacgg atattagctg atttttcaac ataagctgga acgttaccag
    20401 cacctacccc aagggcaggt ttcccacatg agtatgccgc tttaaccatc gcatttccac
    20461 ctgtcgcaag gatagtggca atgccgtcat gattcatcaa agcatttgtt gcttcaagtg
    20521 atggtgtttc aacccactgc acacaatttt caggagcacc agcggcaata gcagcatcac
    20581 gaacaatacg agcggcatgt gctgatgatt cttgtgctga tgggtgaaag gcaaaaataa
    20641 ttggattacg tgtttttaac gagatcaaag atttaaagat agctgttgaa gttgggttag
    20701 ttgtgggtgt aatcccacag ataacaccca ccggctcagc aattaatgtc aaacctgtca
    20761 catcatcttc ttcaataatt cctactgttt tttgatggcg catgttatta accacatgct
    20821 cacaagcaaa tagatttttt gttgctttat cttcaaagac accgcgacct gtttcttcat
    20881 aagcatgttt agcaagttca ccgtgagcat ccaaggcagc aacagaagct ttggctacga
    20941 tataatcaac ttgttcttgg tcaagcttac gcatctcttc aagagcagct aaaccttttt
    21001 gaacaagagc attaattgtc acagaaacag aggttgtttc aacagtatta tgtccttcag
    21061 tcatttgaga ctcctttatc ttgttaaaag
//
LOCUS       DATXQK010000025        20719 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.025, whole genome shotgun sequence.
ACCESSION   DATXQK010000025 DATXQK010000000
VERSION     DATXQK010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20719
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.025"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(115..1251)
                     /gene="dnaJ"
                     /locus_tag="VC394_001071"
     CDS             complement(115..1251)
                     /gene="dnaJ"
                     /locus_tag="VC394_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEP5994546.1"
                     /translation="MNNTEYYDRLGVSKDASQDDIKKAYRKMSKKYHPDINKEAGAEQ
                     KYKDVQEAYETLSDSQKRAAYDQYGAAGAQGGFGGGAGGFGGFDGGGFGGFEDIFSSF
                     FGGGGSRNPNAPRQGDDLQYRVNLSFEEAVFGVEKEVSYNREATCGTCLGSGAKPGTA
                     PVTCRKCHGSGVMTIDTQTPLGMMRRQVTCDICHGSGKEIKEPCQTCHGTGHEKQAHK
                     VSVKIPAGVETGQQIRLQGQGEAGFNGGPYGDLFVILNVLPSKQFERNGSTIYYNLDI
                     SFTQAALGDTVEIPTVHGDVEMAIPAGTQTGKTFRLKGKGAPKLRGGGQGDQHVTVNI
                     VTPTKLNDAQREALQAFAEASGEKMLHPKKKGFFDKVKDALEDI"
     gene            complement(1532..3358)
                     /gene="dnaK"
                     /locus_tag="VC394_001072"
     CDS             complement(1532..3358)
                     /gene="dnaK"
                     /locus_tag="VC394_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEP5994547.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPETVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGMDKTDK
                     DEKILVFDLGGGTFDVSILELGDGVFDVLATAGDNKLGGDDFDQKIIDFLVAEFKKEN
                     GIDLSQDKMALQRLKDAAEKAKKDLSGVTQTQISLPFITAGSAGPLHLEMSLSRAKFD
                     DLTRDLVERTKTPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQHIVIKSNDGLSEEEIDRMMKDAEANAEADAKRKEEVDLKNE
                     VDQAIFATEKTIKETEGKGFDTERDAAQSALDELKAAQESGNLDDMKAKLEALNEKAQ
                     ALAVKMYEQAVAAQQAAQGAEGAQANDSANNDDVVDGEFTEK"
     gene            complement(3539..4111)
                     /gene="grpE"
                     /locus_tag="VC394_001073"
     CDS             complement(3539..4111)
                     /gene="grpE"
                     /locus_tag="VC394_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEP5994548.1"
                     /translation="MAVFNKLFKRRHSVSEEIKKDDLQEEVEATETEETVEEVIEETP
                     EKSELELANERADEFENKYLRAHAEMQNIQRRSSEERQQLQRYRSQDLAKAILPSLDN
                     LERALAVEGLTDDVKKGLEMTRDSLIQALKEEGVEEVEVDSFDHNFHMAVQTLPADDE
                     HPADSIAEVFQKGYKLHERLLRPAMVVVYN"
     gene            complement(4114..5148)
                     /gene="hrcA"
                     /locus_tag="VC394_001074"
     CDS             complement(4114..5148)
                     /gene="hrcA"
                     /locus_tag="VC394_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001872861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEP5994549.1"
                     /translation="MITQRQNDILNLIVELFTQTHEPVGSKALQRTIDSSSATIRNDM
                     AKLEKLGLLEKAHTSSGRMPSPAGFKYFVEHSLRLDSIDEQDIYHVIKTFDFEAFKLE
                     DMLQKASHILAEMTGYTSVILDVEPARQRLTGFDVVQLSNHDALAVMTLDESKPVTVQ
                     FAIPRNFLTRDLIAFKAIVEERLLDSSVIDIHYKLRTEIPQIVQKYFVTTDNVLQLFD
                     YVFSELFLETVFVAGKVNSLTYSDLSTYQFLDNEQQVAISLRQSLKEGEMASVQVADS
                     QEAALADVSVLTHKFLIPYRGFGLLSLIGPIDMDYRRSVSLVNIIGKVLAAKLGDYYR
                     YLNSNHYEVH"
     gene            complement(5283..5852)
                     /locus_tag="VC394_001075"
     CDS             complement(5283..5852)
                     /locus_tag="VC394_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEP5994550.1"
                     /translation="MRKRLKFPYFLTLLACFLLLIVCPLLSSQRIASADKEVRVNYSQ
                     KQFITKMGKEVKPLAKYYGIRPSILIAQILLETHDGKTLLASKYHNLFSKKATPGQAA
                     ITLKSPKQTNQNVRYAIYKDDASAIRDYLRMLRQGKEVDKRLYRNLATEKGYKAPAKS
                     LQKYLHYTDKTYARRLIQVIESNDLTNYD"
     gene            complement(5849..6592)
                     /locus_tag="VC394_001076"
     CDS             complement(5849..6592)
                     /locus_tag="VC394_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEP5994551.1"
                     /translation="MKHNHFKLLLQTLVVLLFVGGIYLFIKPEKQATKTPSSQQASSQ
                     KVVKNSKESTGLPKVSPKDWELILVNRDHMTEELSPELVDINGISVDKRIEQATSDFL
                     AAAQAIDPQEHLISGYRSVAYQAELYQSYIKKEMANDPTLTQEAAEALVQTYLQPPGA
                     SEHHTGLAIDMSTVDTLNASDPSVSKAVQKIAPDYGFVLRFPEGKKTSTGVDYEDWHY
                     RYVGKASARYMAQHNLTLEEYIAALKEKR"
     gene            complement(6582..7289)
                     /locus_tag="VC394_001077"
     CDS             complement(6582..7289)
                     /locus_tag="VC394_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEP5994552.1"
                     /translation="MTKTRLYIARHGKTMFNTIGRAQGWSDTPLTKKGEEGIRELGLG
                     LKDATIPFKAAFSSDSGRTMQTIEIILRESENEFLPYTKDNRIREWCFGSLEGTYDSE
                     LFLGVLPRTKAFENRDNLRDVPYSELAESIVEVDTANWAEPWEVLRKRIWEGFEAIAL
                     SIQNAGGGNALVVSHGMTIGTFMWLIDPDREKQYIDNGSVTVVEFEDGQFTIKTIGDM
                     SYRYRGREIIEAMSDEA"
     gene            complement(7681..7920)
                     /locus_tag="VC394_001078"
     CDS             complement(7681..7920)
                     /locus_tag="VC394_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEP5994553.1"
                     /translation="MIWSLIVGAFIGMIAGKMTEKSQSMGCFTNIIAGLIGSAVGQRL
                     FGDWGIQLAGMAIFPSILGAAIVIVLVSAIFGKRY"
     gene            complement(8139..8354)
                     /locus_tag="VC394_001079"
     CDS             complement(8139..8354)
                     /locus_tag="VC394_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994554.1"
                     /translation="MIVADSNKLKYFVSEKKAVGLSQAAFLYVMLVTVYKKTIIYAKI
                     RLTGIALEPSGVSDVYLMNYHVCLHYY"
     gene            complement(8351..9790)
                     /gene="gatB"
                     /locus_tag="VC394_001080"
     CDS             complement(8351..9790)
                     /gene="gatB"
                     /locus_tag="VC394_001080"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEP5994555.1"
                     /translation="MNFETIIGLEVHVELNTNSKIFSPSSAHFGEDPNANTNVIDWSF
                     PGVLPVMNKGVIDAGIKAALALNMDIHKEMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEIKLEDGSTKKIRIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEIIQYTGISDVKMEEGSMRVDANISLRPYGQEQFGTKTELKNLNS
                     FSNVRKGLEFEVERQAKLLRSGGVIRQETRRYDEANKGTILMRVKEGAADYRYFPEPD
                     LPLYEIDDAWIDEMRAQLPQFPAQRRAKYEEELGLSAYDASQLTATKALSDFFETAVS
                     LGGDAKQVSNWLQGEVAQFLNAEGKTIEEIRLTPDNLVEMIAIIADGTISSKMAKKVF
                     VHLAKNGGSARAYVEKAGLVQISDPAVLVPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVAQQLLAQELQKLRD"
     gene            complement(9790..11256)
                     /gene="gatA"
                     /locus_tag="VC394_001081"
     CDS             complement(9790..11256)
                     /gene="gatA"
                     /locus_tag="VC394_001081"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEP5994556.1"
                     /translation="MSFNHKTIEELHDLLVAKEISATELTQKTLEDIKSREEAVGSFI
                     TVSEEAALKQAAAIDAKGIDADNLMSGIPLAVKDNISTKGILTTAASKMLYNYEPIFD
                     ATSVANAYAKDMIVIGKTNMDEFAMGGSTETSYFKKTKNAWDHTKVPGGSSGGSATAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGVVGLKPTYGTVSRYGLIAFGSSLDQIGPFAPTV
                     KENAQLLNVIASSDVKDATSAPVRIADYTSKIGRDIKGMKIALPKEYLGEGIDPEIKE
                     TVLAAAKQFEALGATVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGFRADDA
                     KNLDEIYVNTRSQGFGDEVKRRIMLGTFSLSSGYYDAYFKKAGQVRTLIIEDFDKVFA
                     DYDLILGPTTPTVAFGLDTLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFVDGLPVG
                     LQLIGPKYAEETIYQAAAAFEAVTDYHKQQPIIFGGDK"
     gene            complement(11256..11558)
                     /gene="gatC"
                     /locus_tag="VC394_001082"
     CDS             complement(11256..11558)
                     /gene="gatC"
                     /locus_tag="VC394_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008086915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEP5994557.1"
                     /translation="MKISEEEVRHVAKLSKLSFLESETTTFATTLSKIVDMVELLNEV
                     DTEGVAITTTMADKKNVMRQDIAEEGTDRALLFKNVPEKENHFIKVPAILDDGGDA"
     gene            complement(11790..12108)
                     /locus_tag="VC394_001083"
                     /pseudo
     CDS             complement(11790..12108)
                     /locus_tag="VC394_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991537.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dikinase"
     gene            complement(12552..13106)
                     /locus_tag="VC394_001084"
     CDS             complement(12552..13106)
                     /locus_tag="VC394_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEP5994558.1"
                     /translation="MRALISIDYTNDFVADDGKLSAGKSAQAIATKIAEVTKTAFDQG
                     DYIFFAIDCHDQNDPWHPESKLFAAHNIKGTTGRHLYGPLAEVYSYMKQHPRVFWIDK
                     RYYSAFSGTDLDIRLRERGITQLVLTGVLSDICVLHTAIDAYHLGYQLEIVKSAVASL
                     TKESYEWSLAHFEQVLGAKLINDY"
     gene            complement(13253..14035)
                     /gene="codY"
                     /locus_tag="VC394_001085"
     CDS             complement(13253..14035)
                     /gene="codY"
                     /locus_tag="VC394_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001133183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEP5994559.1"
                     /translation="MPNLLEKTRKITSILQRSVDSLETELPYNTMASRLADIIDCNAC
                     IINGGGTLLGYAMKYKTNTDRVEEFFEAKQFPDTYVKAASRVYDTEANLSVENELTIF
                     PVESKDTYPGGLTTIAPIYGGGMRLGSLIIWRNDNEFSDDDLILVEISSTVVGIQLLN
                     LQTENLEDTIRKQTAVNMAINTLSYSEMKAVAAILGELDGNEGRLTASIIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVINEGIFAKLKEF"
     gene            complement(14253..15467)
                     /locus_tag="VC394_001086"
     CDS             complement(14253..15467)
                     /locus_tag="VC394_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEP5994560.1"
                     /translation="MKIIEKSSKLEHVAYDIRGPVLDEANRMIASGEKILRLNTGNPA
                     AFGFEAPDEVIRDLIVNARLSEGYSDSKGIFSARKAIMQYCQLKGFPDVDIEDIYLGN
                     GVSELISISLQALLDNGDEVLVPMPDYPLWTACVSLGGGKAVHYLCDEEAGWYPDIAD
                     IKSKITSRTKAIVVINPNNPTGALYPKEILEDIVALAREHQLIIFADEIYDRLVMDGK
                     EHIAIASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKDHVKGYIEGLNMLANMRL
                     CSNVLAQQVVQTSLGGHQSVDALLLPGGRIFEQRNFIYEAINAIPGLSAVKPEAGLYL
                     FPKIDRQMYRIDDDEEFVLQLLKQEKVMLVHGRGFNWKDPDHFRIVYLPSVEELEDVQ
                     EKITRVLYQYRR"
     gene            15698..16150
                     /locus_tag="VC394_001087"
     CDS             15698..16150
                     /locus_tag="VC394_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015055992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEP5994561.1"
                     /translation="MSLKYKRILVAIDGSYESELAFNKGVNVALRNDATLLLVHVIDT
                     RALQSVATFDTYIYEKLEQEAKDVLDDFEKQAQIAGITNIKQIIEFGNPKNLLAHDIP
                     DRENADLIMVGATGLNTFERLLIGSSSEYIMRHAKIDLLVVRDSTKTL"
     gene            complement(16273..17661)
                     /locus_tag="VC394_001088"
     CDS             complement(16273..17661)
                     /locus_tag="VC394_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEP5994562.1"
                     /translation="MTVKAVFFDIDGTLLNDRKNIQKTTQKAIQQLKKQGIMVGLATG
                     RGPGFVQPFLENFGLDFAVTYNGQYILTRDKVLYQNQLPKSMIYKVIRYANEKKREIS
                     LGTASGLAGSRIIDMGTSPFGQVISSFVPKSWARTVEGSFKHLIRRIKPQSFRNLVTI
                     MREPIYQVVLVASQAETKKIQEKFPHIKITRSSPYSLDLISVDQSKIKGIERLGEMFG
                     FDLSEVMAFGDSDNDLEMLSGVGIGIAMGNAETVVKDGAHFTTDSNNNDGISKALAHY
                     GLIHFDIEKSFKSRDENFNKVKDFHRLMDSDTIETPRSYTISEAGYRSGFKVEEIVEF
                     LYAASKGDQQQFTQAIFDLHGAVDQAANKVQAKKHVETPLIGQVDALADLLYFTYGSF
                     VLMGVDPQPIFEAVHEANMAKIFPDGKAHFDPVTHKIQKPDYWQERHAPEVAIKKELD
                     KQLQKSLQRFDK"
     gene            17733..18698
                     /locus_tag="VC394_001089"
     CDS             17733..18698
                     /locus_tag="VC394_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEP5994563.1"
                     /translation="MKKILVLHTGGTISMQADNSGRVVPNQDNPMTKIHAAAQDIQLT
                     VSDFLNLPSPHITPHHMLSIYHHIQERTDVFDGIVITHGTDTLEETAYFLDTMALPTN
                     IPVVLTGAMRSSNEVGSDGIYNYLTALRVASSDKAKEKGVLVVMNDEIHAAKYVTKTH
                     TTNISTFQTPTHGPLGIIMKNDLLFFKTAEPRIRFDLRCISGTIPIIKAYAGMGDGSI
                     LSLLTPGSIQGLVIEALGAGNVPPLAVGEIEHLITLGIPVVLVSRCFNGMAEPVYAYE
                     GGGAMLQEAGVMFVKELNAPKARLKLLIALNAGLTGQELKDYIEG"
     gene            complement(19047..19751)
                     /locus_tag="VC394_001090"
     CDS             complement(19047..19751)
                     /locus_tag="VC394_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP5994564.1"
                     /translation="MISYLKADLFRIRQERLALFSCMLLVVIAVGVAYSFRKTPNQVI
                     MIQIINIITDFVTLFFLTPARIFFSDDYNYRTINNVVVKQQNRTLIIIYKLLVSFGLS
                     LLFISLTYLLSALAFHLFTGQAYYGIVIDRMIKQLPFYIAIIALCQFLFNILRKTYQS
                     ITAYILYVLLFDKVSATLANHFFKLDISPVLMFKHLSLSLGDKHLSSTSILVAFGLTL
                     CYLLGNVFLFSKRELK"
     gene            complement(19764..20666)
                     /locus_tag="VC394_001091"
     CDS             complement(19764..20666)
                     /locus_tag="VC394_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5994565.1"
                     /translation="MTQQSMLLSIEQVNKSYGKNQVLSDISFDIYKGEICGLVGQNGA
                     GKTTLMRILSGLIGKDSGQIKQLQPYRMGSIIESPTLYPNMTAHDNLYYAALQLRLAD
                     AKERIHEVLELIGLEKVSKKKKVKDYSLGMRQRLAIGLSILDFPEFLILDEPINGLDP
                     AGIKEMRQIILNLRDCYGITILISSHILSELDLVVDRYVIMHKGKVIKSMDKAELKAQ
                     VKVQIALHTSDDQLVKNRLLELGLRVETDGQMLLIKPTLSVMELIKIVLDLPVEIFDI
                     YHHQVSFEHYYLDLLGKDAASPLI"
BASE COUNT         6298 a         4382 c         3585 g         6454 t
ORIGIN      
        1 tttgacaaga gaaaaaaacg atatctataa aagaaaacgt tttctctaac tgttttgacg
       61 taaaaaaacg tataacttcg ggaaaattat cctgagatta tacgtttgac atctttaaat
      121 atcctctaag gcatctttca ctttatcaaa aaaacctttt ttctttggat gtaacatttt
      181 ttcaccactc gcttctgcaa atgcttgcaa ggcttccctt tgagcgtcat tgagtttggt
      241 tggggtcaca atattaacgg taacatgttg atccccttga cctccaccac ggagttttgg
      301 tgctcctttg cctttgaggc ggaaggtttt acctgtttgt gtcccagcag ggatagccat
      361 ctcaacatcg ccatgaaccg ttggaatttc aacagtatct cctaaggctg cttgagtaaa
      421 agaaatatct aagttgtagt aaatagtgga accattgcgt tcaaattgct tacttggtaa
      481 gacattcaaa ataacaaata aatcaccata aggtccgcca ttaaagccag cttctccttg
      541 accttgtaaa cggatttgct gaccagtctc tacaccagct ggaatcttaa cagacacttt
      601 atgagcttgc ttttcatgac cagtaccgtg acaggtttga cagggttctt taatttcctt
      661 accactacca tgacaaatat cgcaggtgac ttgtcgacgc atcataccaa gtggtgtttg
      721 ggtgtcaatt gtcattacac ctgaaccatg gcacttacga caagttactg gagcggtacc
      781 aggttttgca cctgagccta aacaagtgcc gcaggttgct tcgcgattat agctaacttc
      841 tttttcaaca ccaaatacag cctcttcaaa agataaatta acacgatatt gcaaatcgtc
      901 accttgacga ggggcatttg ggtttcggct tccaccgcca ccaaagaagc ttgagaagat
      961 atcttcaaaa ccaccaaagc cacctccgtc aaaaccacca aagccgcctg ctccgccacc
     1021 aaagccgcct tgagcacctg cagcgccgta ttgatcataa gcagcacgct tttgtgaatc
     1081 acttaaggtt tcgtaagctt cttgaacatc cttatacttt tgctccgctc cagcttcttt
     1141 attgatatct ggatgatatt tcttagacat ttttcgataa gcttttttga tatcgtcctg
     1201 agaagcgtcc ttagaaaccc ccagacgatc gtaatattct gtattgttca taatgtaaga
     1261 taccaagccg taaaacgact caatgaaaaa taggaaatcc gacaaagata ctctcgtatc
     1321 taggaggtat ttctcttttt tcgcagagtc gtaggcgtgt tcgaatccgc aaaagagtct
     1381 ttacacgctt tagcctgtgt tcgaataatc gaacttgtat tcctttctta aaatattttg
     1441 acgtcagcat ttttacattt tatttagcca aaaacagagg tttggtacaa cctctgaatt
     1501 cggatattaa tgttactaga taactaaatc attacttttc tgtaaattcg ccatctacaa
     1561 catcatcatt atttgctgaa tcattagctt gtgcaccttc tgcaccttgt gctgcttgtt
     1621 gagctgctac agcttgctcg tacattttaa cagccaaagc ttgcgctttt tcatttaatg
     1681 cttcaagttt agctttcatg tcgtcaaggt tgccagattc ttgcgcagct tttaactcgt
     1741 caagagctga ttgcgctgca tcgcgttctg tatcaaagcc tttaccttca gtttctttga
     1801 ttgttttttc agtagcaaag atagcttggt caacttcgtt tttaaggtca acttcttctt
     1861 tacgtttcgc atcggcttcg gcattagctt cagcgtcttt catcatgcga tcaatttctt
     1921 cttcagaaag tccgtcgttt gatttgataa cgatgtgttg ttccttttgc gtaccaaggt
     1981 ctttagcttt tacagaaaca ataccgtttt tatcgatatc aaatgttact tcaatttgtg
     2041 ggattccacg aggtgcagct gggatatcag tcaattggaa gcgaccaaga gtcttgttat
     2101 ctgctgccat tgggcgttca ccttgaagaa catggatatc aacagctggt tggttgtctg
     2161 ctgctgttga gaagacttgt gatttagatg ttgggatagt ggtgttgcgg tcgatcaatt
     2221 tggtgaagac accacccatt gtttcaatac caagtgacaa tggtgttacg tcaagaagga
     2281 caacgtcttt cacatcccca gtgataacac caccttggat agcagcaccc atagcaacca
     2341 cttcatcagg gtttacagat ttatttggtt ctttaccagt ttcagctttt acagcttcga
     2401 caactgctgg gatacgagtt gatccaccaa caaggataac ttcatcaatt tctgacaatg
     2461 acaatcctgc atctgaaagg gcttgacgaa ctggagtttt cgtacgttct acaaggtcgc
     2521 gagtgagatc gtcaaattta gcacgagata agctcatctc caagtgaaga ggaccggcag
     2581 aaccagcagt gatgaacggt aatgaaattt gtgtttgtgt cacacctgaa agatcttttt
     2641 tagctttttc agcagcatct ttcaagcgtt gaagtgccat cttatcttgt gataagtcaa
     2701 tgccattttc tttcttaaat tcagccacta agaaatcaat aattttttgg tcaaagtcgt
     2761 caccaccaag tttgttatca cctgctgttg caagaacgtc gaagacacca tcacctaatt
     2821 caaggattga tacgtcaaat gtaccaccac caaggtcaaa aactaagatt ttttcatcct
     2881 tgtcagtctt gtccatacca taagcaagtg cggctgctgt tggttcgtta acgatacgtt
     2941 ctacttcaag acctgcaatt ttaccagcgt ctttagttgc ctgacgttgt gcatcgttga
     3001 agtaagctgg aacagtaata actgcttttt ctactttttc tccaagatag tcttcagcat
     3061 aacctttaag gtattgaaga atcattgctg aaatttcttg aggagtatat tctttaccat
     3121 ttgcagaaac tttttcagaa gttcccattt tagatttaat agagattact gtttctgggt
     3181 ttgtcactgc ttgacgtttt gcagcatcac ccacgataat ttcaccattt ttgaatgata
     3241 ctactgaagg agttgtacga ttgccttctg ggttagcaat gatttttgat tcagtccctt
     3301 caagaactgc tactgctgag tttgttgtac ctaagtcaat accaataatt ttagacatgt
     3361 taatacctct tattttataa tactttatta ctttttattt caatttatct tagtaagcac
     3421 tcgaattatt ttacttccaa ttctaagcaa tcactatagt ttaatcagca agcgaaataa
     3481 cttctaactt gctatctagt ttactgtcat tacggacaaa aacgaaaact catttacctt
     3541 agttatagac aactaccatc gctggtctta gcagacgctc gtgcaattta tatccctttt
     3601 gaaagacttc tgcaatgcta tcggctggat gttcatcatc ggctgggagg gtctgaactg
     3661 ccatatgaaa gttgtggtca aatgagtcaa cttctacttc ttctacgcct tcttccttta
     3721 gtgcttgaat aaggctatca cgagtcattt ctaaaccttt tttaacatca tctgtcaacc
     3781 cttcaacagc caaggcacgt tctagattgt caagcgatgg taagattgct tttgccagat
     3841 cctgagaacg atagcgttga agttgttgac gttcttcact agagcgacgc tgtatattct
     3901 gcatttctgc atgagctcgc aagtatttat tttcaaactc atcagcacgt tcgtttgcaa
     3961 gttctaactc tgatttttca ggagtttctt caataacttc ctcaactgtc tcttcagttt
     4021 cagtggcttc cacttcttct tgtaaatcgt cttttttaat ttcttctgac actgagtgcc
     4081 tccttttaaa taacttattg aatactgcca ttatcagtgg acttcataat ggttagaatt
     4141 taggtatcga taataatcac ctaatttagc agccaatacc tttccaataa tgtttaccaa
     4201 actaacagac ctacgataat ccatatcaat aggaccaatc aaactcagga gtccaaaccc
     4261 acggtaagga atcaaaaatt tgtgagtcaa aactgacaca tctgcaagtg cggcttcttg
     4321 actatctgcc acttggacag aagccatctc accttctttc agactttgcc tcaaagaaat
     4381 tgctacttgc tgttcattat ccaaaaactg atacgttgat agatctgaat acgttaaaga
     4441 atttacctta ccagcaacaa ataccgtttc caaaaacagc tctgaaaaaa cataatcaaa
     4501 tagttgtaaa acattatccg tagtcacaaa atacttttga acgatctgag gaatttctgt
     4561 cctcaactta taatgaatat ctatgacact actatccagc aagcgctcct ctacgatagc
     4621 tttaaatgct atcaagtcac gtgtcaaaaa gtttcttgga atagcaaact gcactgtcac
     4681 tggcttagat tcatctaaag tcatgactgc tagagcatca tggttgctaa gctgaacaac
     4741 atcaaaccct gttaaacgtt gacgagctgg ctccacatct aaaataacgg atgtatagcc
     4801 cgtcatctcc gctaaaatgt gactagcttt ttgaagcatg tcttctaatt tgaatgcttc
     4861 aaaatcaaaa gtttttatga catgataaat atcttgctca tcaatactat ctagcctgag
     4921 ggagtgctct acaaaatact taaagccagc tggacttggc atccgtccac tagaagtgtg
     4981 cgctttttcc aacaatccca acttctctag ttttgccata tcattacgaa tagtggcgct
     5041 actagaatca atcgttctct gtaaagcttt agaccctact ggttcatgag tttgtgtaaa
     5101 tagctcaacg attaaattca aaatatcatt ttgacgttgt gtaatcacaa accaaccact
     5161 ccctttcgtt tagcactcta tttagtagac tgctaattta taattctatt atactcttta
     5221 tgggacacat gtcaagaaaa aaactcaaaa aaattagcac tcttttataa agagtgctaa
     5281 tttcagtcat agtttgttaa atcgttactt tcaatgacct gaatcagtcg cctagcatag
     5341 gttttatcag tataatgcaa atacttttgt aaacttttag ctggtgcttt ataccctttt
     5401 tctgtagcaa gattacgata caaacgctta tcgacttctt ttccctgccg aagcattcgt
     5461 aaataatctc taattgcact agcgtcatct ttataaatag catatctcac gttttggttg
     5521 gtttgtttag gggactttag ggtaatggcc gcttgtcctg gagttgcttt cttgctaaaa
     5581 agattatgat acttagacgc tagtaatgtt tttccatcat gggtttccaa aagaatttga
     5641 gcaatcaaaa tagatggtct aatgccataa tattttgcca atggtttaac ttctttaccc
     5701 atttttgtaa taaattgttt ttggctgtag ttcaccctta cttctttatc tgcactagct
     5761 atcctttggc tactcagtaa gggacaaaca atcagcaata aaaagcaagc tagaagcgtt
     5821 aaaaaatacg gaaactttag tcgttttctc atcgtttctc ctttaaagca gcaatgtact
     5881 cttccaacgt taggttgtgc tgagccatat aacgagcaga cgccttgcct acatagcggt
     5941 aatgccaatc ctcataatca acccctgtac tagtcttttt tccttctgga aagcgcaaga
     6001 caaacccata atcaggtgca attttttgaa ctgctttaga taccgatggg tcactggcat
     6061 ttaaagtatc tacagtgctc atgtcaatag caagaccagt gtgatgttca ctagcccctg
     6121 gcggctgtaa ataggtttgc accaaagcct ccgcagcttc ttgtgtcaat gttggatcgt
     6181 tagccatctc ttttttaata taagattggt aaagctctgc ttggtaagct acggagcgat
     6241 agcctgaaat aaggtgctct tgtgggtcaa tagcctgagc tgccgctaaa aaatcactag
     6301 tggcttgctc aatgcgttta tccacagaaa taccattaat gtcaactaat tctgggctca
     6361 attcctcagt catatggtcc cgattcacta aaatcaattc ccaatctttt ggagagactt
     6421 taggaagccc tgtggactct ttgctatttt tgaccacttt ttgactgctt gcctgttgac
     6481 ttgaaggcgt cttggtggcc tgcttctctg gcttaataaa gaggtaaata cctccgacaa
     6541 agagcagaac aaccagtgtt tgcaataata atttaaaatg attatgcttc atctgacatt
     6601 gcttctatta tttcacggcc acggtagcga taactcatat ctccaatggt tttgatggta
     6661 aattgcccat cttcaaattc gacaacagtg acactgccgt tatcaatata ttgtttctca
     6721 cggtcaggat cgattagcca cataaatgtt ccaatagtca tcccatggct aaccacaaga
     6781 gcatttccgc cgccagcatt ttgaatagac aaagcaatag cttcaaaccc ctcccaaatg
     6841 cgcttacgaa gaacttccca tggttctgcc caattagcgg tatctacttc cacgatgctt
     6901 tctgccagtt cagaataggg aacatcacgt aggttatcac gattttcaaa ggcttttgtt
     6961 cttggaagga cacctagaaa taattctgaa tcataagtcc cttctagact cccaaaacac
     7021 cactcacgaa tacgattatc tttagtataa ggcaaaaatt cgttttctga ttctcttaaa
     7081 ataatttcta ttgtctgcat ggttcgacct gaatcacttg aaaacgcagc cttaaatggt
     7141 atagttgcat cttttaaacc taagccaagt tctcgaatac cttcttctcc tttttttgta
     7201 agaggtgtgt cactccaacc ctgagcgcga ccaatggtat taaacatcgt ttttccatgt
     7261 ctggcaatat acaaccttgt ttttgtcatt tcaacctcct aaactgtctt attaattatt
     7321 atagcattta aagaaaacac ttgcaaaaca cttcctgata gctatttgag atcaaataga
     7381 ccactgactt attgtctatt ttattaaaaa taatgctatt gctacaattc aaatcatcac
     7441 ataaatttcc aaacgattac atttgtgcgt ttcgctcaaa tatatataac aacagaccca
     7501 cactacaact aaactgacat tacaaaatga tgtcaaaaca gtaacaaaat ctaactattt
     7561 gtcaagaaaa tgatattaaa ttttcacaca actatcttat aatgaggatg caaccaataa
     7621 aaagataaga cattttatgt tgcaaaaaag gctgcttgga aacttccatg cagccttttt
     7681 ttaatacctt tttccaaaaa tagctgaaac aagtacaatt acaattgccg ctcccaaaat
     7741 ggacgggaaa atagccatac ctgccaactg aattccccaa tccccaaaaa gcctttgccc
     7801 aacagctgac cctatgagac ctgcgataat attagtaaaa caacccatgg actggctttt
     7861 ttcagtcatt ttacctgcta tcatgccgat aaaggcacct acaattaaag accatatcat
     7921 cgttaacacc tcctttcttg taaaatcctt ttagaagaac tgctatccat taaatgttct
     7981 taactttagt tgattccttt accttcttct ttagtaaatg tttccgtgcc tgagtaaaat
     8041 agaaaaaaca attccttcta ttaaggtaga tagaccgaga acgactagaa gtcttcacac
     8101 actaaataac ttaacaaact ttatcacaca gctaatcgct aataatagtg tagacaaacg
     8161 tgatagttca ttaaatacac gtcagaaact cctgaaggtt ctaaagcaat acctgtcagt
     8221 cttattttag catagatgat ggtcttttta tagacggtca ctaacatgac atacaaaaag
     8281 gcagcctgtg aaagaccgac cgcctttttt tcagagacaa aatactttaa cttattgcta
     8341 tcagcaacta tcaatctcgc aatttttgca attcttgtgc caataattgt tgagcgactt
     8401 gtggattagc ttgacctttg gttgccttca tcaagaagcc tgtgaaagcc ttatcagcat
     8461 tacgtttgcc tgatttgaaa tcagccactg cagcttcatt atcagcaaag acttggtgaa
     8521 taattggtac caaaacagcg ggatcagaaa tctgaacaag tcctgccttt tcgacgtagg
     8581 cgcgagctga gccaccgttt ttagccaaat gcacaaagac cttcttagcc attttagaag
     8641 agattgtacc atcagcaata atagcaatca tttccactaa gttgtcaggc gtcaatctaa
     8701 tttcttcaat ggttttgcct tctgcattta agaattgggc aacttcccct tgcaaccagt
     8761 tagagacttg cttggcatcg ccaccaagac ttactgcagt ttcaaagaaa tctgacaagg
     8821 ctttggtagc tgttagttgg ctagcatcat aagctgagag gccaagttcc tcttcgtatt
     8881 tagcacgacg ctgagctggg aattgaggta attgagcacg catctcgtca atccaagcat
     8941 catcaatctc atataatggc aggtctggtt ctgggaaata acgatagtcc gctgcccctt
     9001 ctttgacccg catcaaaata gtccccttat tcgcttcgtc atagcggcgc gtttcttggc
     9061 gtatcacacc acctgaacgc aagagtttgg cttgacgttc cacctcaaat tcaagccctt
     9121 tacggacatt tgagaaggag ttcaagtttt tcaattcggt ctttgttcca aattgttctt
     9181 gcccgtaagg acgaagagaa atattggcat ccacacgcat ggagccttct tccatcttga
     9241 catcagaaat acccgtatac tggatgattt ctttcagagc tgtcaaataa gcataggctt
     9301 cttcaggaga acgcatgtcg gcttctgaca caatctcaat caaaggaact ccttggcggt
     9361 taaggtccac ataagaataa ccatctgtgc catgagtgtt tttaccagca tcttcttcca
     9421 agtgtgcgcg ttcaatacga attttcttag tcgagccgtc ttccaattta atctcaatcc
     9481 atccgttgta gccaatcggc tcgtcaaact gtgaaatttg gtaagcttta ggattgtctg
     9541 ggtagaaata atttttgcgg tcgaaatgca tctctttatg gatgtccata ttgagggcaa
     9601 gcgcagcttt tatgcccgca tcaatcaccc ctttgttcat gactggtaag acccctggga
     9661 aagaccaatc aatcacattc gtgttggcat ttgggtcctc accaaagtga gctgacgaag
     9721 gtgagaaaat tttggaattg gtatttaatt ccacatggac ttcaagccca ataatggttt
     9781 caaaattcat tacttgtcac ctccaaaaat aatcggttgt tgcttgtgat aatctgtcac
     9841 cgcttcaaag gctgctgccg cctgatagat ggtttcttca gcgtatttcg gaccaattaa
     9901 ttgaagccct actggcaagc catcgacgaa tcctgctgga atggaaattc ctggcagacc
     9961 agccaagtta actggaatgg tcaataagtc ggccaaatac atggcaacag gatcgtggtt
    10021 gagtgtgtct aagccaaaag caaccgtcgg tgttgttggg cctaaaataa gatcataatc
    10081 cgcaaagacc ttgtcaaaat cttcaataat caaggtacgc acttgaccgg cctttttgaa
    10141 ataagcgtca taataacctg atgacaaact aaaggtccca agcatgatac gacgtttaac
    10201 ctcatcacca aagccttggc tacgagtgtt aacatagatt tcatccaagt ttttagcatc
    10261 gtcagcacgg aaaccatagc gaatgccatc aaaacgttgt aagtttgagg acgcttctga
    10321 cgaagcaata atgtaataga cagccacacc atatttgcta tgtggaagac taacttcctc
    10381 aacagtagct cctaaggctt caaattgttt agctgccgca agaaccgttt ccttaatctc
    10441 aggatcaatc ccttcgccaa gatactcttt aggaagggca atcttcatgc ctttaatgtc
    10501 acgaccaatc ttagatgtgt agtctgcaat gcggacaggc gctgaagtgg catccttgac
    10561 atcgctactt gcaatcacat ttaataattg ggcgttttct ttaactgtcg gagcaaaagg
    10621 tccaatctga tcaagtgaac ttccaaaagc aatgagacca tagcgagaaa cagtgccata
    10681 ggttggtttc aaaccgacaa caccattaaa tgcagctggc tgccgaatag aaccaccagt
    10741 gtctgaacca agagataaac ggacttgtcc tgaagcgact gctgttgccg aaccacctga
    10801 tgagccacca ggtacttttg tgtggtccca agcatttttc gttttcttga aataagaggt
    10861 ttctgttgaa ccacccatgg caaattcgtc catgttggtt tttccaatga caatcatgtc
    10921 tttagcgtag gcatttgcta ctgatgtggc atcaaaaatc ggctcgtagt tatacaacat
    10981 ttttgaagcg gctgtggtta ggatgccctt ggttgagata ttatccttaa cagctagtgg
    11041 aatacccgac atgaggttat cagcatcaat acctttggca tcaattgccg cagcttgttt
    11101 aagagcagct tcttctgaaa cagtgataaa cgacccgaca gcctcctcac gagacttgat
    11161 gtcttcaagt gttttttgag tcaattcggt tgcggaaatc tccttagcta caaggaggtc
    11221 atgcaactct tcaatggttt tatgattaaa agacattagg catctcctcc gtcatccaaa
    11281 atagcaggta ccttgataaa gtgattttct ttttcaggaa catttttaaa gagcaaggca
    11341 cggtctgttc cttcctcagc aatatcttga cgcatcacat ttttcttatc tgccatggtt
    11401 gttgtaatgg cgacaccttc ggtgtcaact tcgttcagca attcaaccat atcaacgatt
    11461 ttggataagg tcgttgcaaa ggttgttgtc tcactttcta agaatgataa ttttgatagc
    11521 ttcgcgacat ggcgaacttc ttcttcagaa attttcatga tttctcccat ccaagatgat
    11581 cagaagtatt ccaattccaa agaacagctc tgctattctc caaaggtagc ggaaaaactc
    11641 ctcacatcgt ttttacatag tctcaattat tatatcacaa ttgcgcccat ttaccatagg
    11701 tgactaatgc aattctagaa agctatacag caaaaaactt cctgactaag gtcagaaaac
    11761 cttagtcagg aagtgtgaca aactaaactc tatttgtctt gggtaagact gtggcctgaa
    11821 gctttgatgg cagcctgagc agccgccaag cgagtaagcg gcactctaaa tggcgaacaa
    11881 gaaacgtagg tcaggccttg gctgtggtaa aagtcaatgg aagcaggatc gccaccatgt
    11941 tccccacaaa taccaagttt caactcaggc ttaaccgaac gggctaaaga gacggccaat
    12001 ccctttctga tcaaaggtct ggaacggctc cttatcaaaa atacccctgt caacgtattc
    12061 ttccaagtat tttcctacaa catcacagaa aaagtaacgt tctttcaagt cggtaatatg
    12121 tttacgaagg tagtaaaggg gtgctttatt ctaatttacc aaatcctcaa cgattgtgac
    12181 aacatgtgat gtgataagcc taagataaaa atggtacaga ctctagcatt caagagtttg
    12241 aagctttatt tatattctaa tgctgaaaag actagagttt gtctaatggc tgtgagacga
    12301 tcctctaaac caccaactca aatcatttct caaacagcta aaatcctgtc tccttcaaaa
    12361 aaacatgtgt tctatctgac acagtccaaa gtttagttgc cttttagaca gcacaagaat
    12421 catcaggact aattctgacc agcatgtcac atacctcatc tacctaaccc atacatacgg
    12481 taaaaccaca tagagcaatg attacggtta ccaaactctt agtcactgaa tcaatagttt
    12541 ggtaggctct tttagtaatc gttaataagc ttagcaccaa gaacctgctc aaagtgagcc
    12601 aaagaccact cataagattc ttttgtaagg ctagcgacag ccgatttaac aatctctaat
    12661 tgataaccaa gatgataagc gtcaatagca gtatgtaaga cacagatatc agataaaact
    12721 cctgttaaca ctaattgagt gatacctctt tctcgtagtc taatatctaa atcagtcccc
    12781 gaaaaagctg aataataacg cttatcaatc caaaaaactc tgggatgttg cttcatataa
    12841 ctgtaaactt cagctagtgg accataaaga tggcgtccag tagttccttt gatattatgt
    12901 gctgcaaata acttactctc aggatgccaa ggatcatttt gatcatgaca atcaatagca
    12961 aaaaaaatat agtctccttg atcaaatgcc gttttagtta cctctgctat tttagtggca
    13021 atagcctgag cagatttacc tgcacttaat ttaccatcat ctgctacaaa atcatttgtg
    13081 taatcaattg aaatcaatgc tctcatcact ctctcctaaa cagggaaacc tctcccctta
    13141 tctcttatat atggcattgt atcagttttt attacaggta tcaagacatc tatttgcatc
    13201 taccattaac agcaaaaaac atctcttttc aagagatgtt gtctttttct atttaaaatt
    13261 cttttaattt agcaaaaata ccttcgttga taactttgag gtatgtccct ttcataccaa
    13321 gagaacgact ttcaataatc cctgcacttt ctaatttacg cagagcattg acaataacag
    13381 aacgggtaat accaatacga tcagcaataa tagaagctgt caatcgtcct tcgttaccat
    13441 ctaactctcc aagaattgct gcaacagctt tcatttctga ataagaaagc gtattaattg
    13501 ccatgttgac cgctgtttgt ttacggatgg tgtcttctaa gttttctgtc tgaagattta
    13561 atagttgaat ccccacaaca gtacttgaga tctcaaccaa aatcaaatca tcatcactaa
    13621 actcattgtc attacgccag ataatgagtg atccaaggcg catccctcca ccataaatcg
    13681 gcgcaatagt cgttagacct cctggataag tgtctttaga ctcaacaggg aatatagtca
    13741 attcattttc gacagaaagg ttagcttctg tatcgtaaac tcggctagca gcctttacat
    13801 aagtatctgg aaattgttta gcttcaaaaa attcctcaac acgatcagtg ttagttttat
    13861 atttcatggc ataaccaagc agtgtgccgc cgccattgat aatacaagca ttgcagtcaa
    13921 tgatatctgc taggcgagat gccattgtgt tatacggtaa ttctgtttct aggctatcta
    13981 cggaacgctg caaaatagat gtgattttac gagttttttc taataagtta ggcatttttt
    14041 tccttttcct ttgaattatt tgcgctttca ttataacaaa gctaactcgc ttttgcaagg
    14101 aattatcaga aaattttcta ttcttactac aattccaact attgatgcct tattatcact
    14161 tcatttaaag gagataagca ctagcttgtt agagcatagg atcacattct tttaagaaac
    14221 gctattttta ttaatcaaag atgagcattg cattaacgtc tatactggta taagacacgt
    14281 gtaatctttt cttggacatc ttctaattct tctacacttg gtaggtaaac aatacggaag
    14341 tgatctggat ctttccagtt aaaaccacgt ccatgtacca acatcacttt ttcttgcttt
    14401 aataactgta agacaaattc ttcatcatca tcaatacgat acatctgacg atcaattttc
    14461 ggaaataaat aaagacctgc ttcaggttta actgccgata agccagggat agcattaata
    14521 gcttcataaa taaaattacg ttgctcaaaa atacgccctc ctggcagtaa cagtgcatca
    14581 actgattgat gacctcctaa ggaagtttga acaacttgtt gggctaaaac attggagcat
    14641 aaccgcatat tggcaagcat gtttaaccct tcaatgtaac ctttaacatg gtctttagga
    14701 cctgataaga ccatccatcc aacacgaaaa ccagcaatac gatgagattt ggaaagacca
    14761 ttcatagata cacaaaaaac atcaggtgcc aaacttgcaa tagcaatatg ctctttacca
    14821 tccatcacca aacgatcata gatttcatcg gcaaaaataa tcagctggtg ttctcttgcc
    14881 aaagcaacga tgtcttctaa aatctcctta ggataaaggg ctcctgttgg gttattagga
    14941 ttgatgacca caatagcttt ggtgcgactc gtgatttttg atttgatatc agcaatatca
    15001 ggataccaac cagcttcttc atcacaaaga taatggaccg cttttcctcc accaagactc
    15061 acacaagccg tccataaagg ataatccggc attggtacta aaacttcatc gccattatct
    15121 agcaaagctt gaagggatat ggatatcaac tctgacacgc catttcccaa ataaatatct
    15181 tcaatgtcaa catctgggaa gcccttgagt tggcaatact gcatgatagc cttacgcgct
    15241 gaaaaaatcc ctttactgtc agaatagccc tcacttagcc tcgcgttgac aattaaatca
    15301 cgaatcactt catctggtgc ttcaaaacca aaagcagcag gattgccagt atttaaacgc
    15361 agaatttttt caccactcgc aatcatgcga ttagcttcat ccaaaacagg ccctcgaata
    15421 tcataggcaa catgttctaa ttttgatgat ttttcaataa ttttcatagt ctcatacacc
    15481 tcgtattcca tattataaac caaaatactt aaaatagaaa gaatttatcc atcaaagctt
    15541 aggacagcta atcgttttaa cacaggtctt atgagacgat aatacagtct ttagtttagc
    15601 aaaaataatg gatcgctttc aaaatgactc aaaaaagagt ataatgaaaa aaagaataaa
    15661 agctattctt gtcaaactgt taaaggagga cagttctatg tcactaaaat ataaacgtat
    15721 cctcgttgct attgatggct cttatgagtc tgaattagct ttcaataaag gcgttaacgt
    15781 agcactacgt aacgacgcta ccttactact agtacacgtt attgataccc gtgccttgca
    15841 aagcgtagct acttttgata cttacatcta tgaaaaatta gaacaagaag ctaaggatgt
    15901 tttagatgat tttgaaaaac aagcacagat agcaggaatt acaaacatca aacaaattat
    15961 tgaatttggc aaccccaaaa acttactagc tcacgatatt ccagatcgtg agaacgctga
    16021 tttaattatg gtaggagcta ctggtcttaa tacctttgaa cgcctcctta ttggctcatc
    16081 ttctgagtac attatgcgac acgctaagat tgacttactt gtagtccgag atagcactaa
    16141 aacactctag tctattacaa ttttcaaaca ccaaaaaaca agcagcatct acaacgaaat
    16201 tgcttgtttt tttgtgctga gctaagtcta gtcaatatct ttttatttct tgatatttaa
    16261 aagagtaaac ctctatttat cgaatcgttg tagtgatttt tgtaattgtt tgtctaactc
    16321 tttttttatg gctacttcag gtgcgtgacg ctcttgccaa tagtcaggtt tttgaatttt
    16381 atgggtaacg gggtcaaagt gtgccttgcc atctggaaaa attttagcca tatttgcttc
    16441 atgtactgct tcaaatatag gttgtggatc tactcccatc agtacaaaag aaccataagt
    16501 aaaataaagc aaatccgcta aggcatcaac ttgcccaatc aggggcgttt caacatgttt
    16561 tttagcttgt accttattag ctgcttggtc aacagcacca tgcaaatcga aaatagcctg
    16621 tgtaaattgt tgttggtctc ccttgcttgc tgcgtaaaga aactcaacaa tttcttctac
    16681 tttaaagcct gacctataac cagcttctga aatggtataa ctacgcggtg tttcaatagt
    16741 atctgagtcc atcagacgat gaaaatcttt gactttatta aaattttcat cacggctttt
    16801 aaagctcttc tctatatcaa aatggatgag accgtagtga gctaaggctt tggaaatccc
    16861 atcattatta ttagagtcgg tagtgaaatg ggctccatct tttaccacag tttcagcatt
    16921 tcccatagca ataccgatac caactcctga taacatttca aggtcattat ctgaatctcc
    16981 aaaagccatc acttcagaca agtcaaatcc aaacatctct cccaaacgtt caattccttt
    17041 tatttttgat tgatcaacag agatcagatc caaggaataa ggactacttc ttgtaatttt
    17101 tatatgaggg aacttctctt gtattttctt agtttcagct tgtgacgcta ccagaacaac
    17161 ctgataaata ggctcccgca taatagttac taaatttcta aaactctgag gcttgattcg
    17221 gcgaatcaaa tgcttaaaac taccctcaac tgttcttgcc caacttttgg gcacaaaact
    17281 actaatgact tgtccaaatg ggctagtccc catatcaata atacgcgatc ctgctaatcc
    17341 agaagctgtt ccaagagaaa tttctcgctt tttttcgttg gcatagcgaa tcactttgta
    17401 aatcatcgac tttggcaatt gattctgata taagacctta tctcttgtta agatatattg
    17461 cccattgtaa gtaacggcaa aatctaatcc aaaattttct agaaaaggtt gtacaaatcc
    17521 cggtccacga ccagttgcta aaccgaccat aatgccttgt tttttgagtt gttggatggc
    17581 tttttgcgtt gttttttgga tatttttacg atcattgaga agggtcccat caatatcaaa
    17641 aaagactgct tttactgtca aaaccttctt ttgcctcctg tgtttatgat agaatagatt
    17701 atatcataaa taaaaggaag tttgacaatc aaatgaaaaa aatcctagtc ttacacactg
    17761 gtggaaccat ttctatgcaa gctgataact ctggtagggt tgttccaaat caagataatc
    17821 ctatgacgaa aatacatgct gcagcccaag atattcagct taccgttagt gattttctaa
    17881 atcttccgag tcctcacatc acgcctcacc acatgttatc tatttatcat catattcaag
    17941 aaagaactga tgtctttgat ggtatagtca tcacacatgg aacagatacc ttagaagaaa
    18001 cagcttattt tttagatacc atggcattgc ctactaatat acctgttgtc ctaacaggtg
    18061 ctatgaggtc ttctaatgaa gttggtagtg atggcatata taattacctt accgcactta
    18121 gagttgcaag tagtgataaa gctaaagaaa aaggggtact tgttgttatg aacgatgaaa
    18181 tccatgctgc taaatatgtt actaaaacac atacaaccaa tatttcaact ttccaaacac
    18241 ccacacacgg accgcttggt atcatcatga aaaatgacct actttttttc aaaactgctg
    18301 aacctcgtat tcgttttgac ttgagatgca tttctggaac catccccatt atcaaagcat
    18361 acgcaggtat gggcgatggt agtatcttga gtctattaac tccaggtagc attcaaggtc
    18421 tagttatcga agctctaggg gcaggaaatg tcccaccatt agcagttggg gagatcgagc
    18481 acttaattac tttaggtatt cctgtcgttc ttgtttcccg ttgtttcaat ggtatggcag
    18541 aacctgtcta tgcttatgaa ggtggtggtg cgatgctaca agaagcaggt gttatgtttg
    18601 taaaagaatt gaatgctcca aaagcccgtc taaagttatt aattgctctc aacgccggac
    18661 ttactggtca agagctaaaa gattatattg aaggataatc tagcttcctt tagaaatgtc
    18721 ccactaaaac atattagtgg gacatttcta gtactttaag ctttaaatta ggacatctcc
    18781 taaatgtcta gaataaacaa ctatgtttca aactatgaga ttgtgcactc ataatcattt
    18841 ctataacagt ttgagaccag tattccaata aatccaactc ttagcaatca gataccgggt
    18901 aaacaagtct ttattttgtc tcattaagta tgagcttact tgaataataa caaaactact
    18961 tgcttatcct tttttagtaa tgatgaagac gacctttttc taagaacttc tttgtctttt
    19021 aaaataaaca aagcacatat taccctttat tttagttctc gtttggaaaa aagaaagaca
    19081 ttgcctaaaa gataacaaag agttaaacca aaggccacta atatgctagt actagaaagg
    19141 tgtttatcac caagagacaa ggataggtgt ttaaacatca aaactgggct aatatctaac
    19201 ttaaaaaaat ggtttgctaa agtagcagat actttatcaa aaagaaggac ataaagtata
    19261 taagctgtga ttgactgata agttttacga agtatattaa acaaaaattg acacaaagca
    19321 ataatagcaa tataaaaagg cagttgttta atcatccgat ctatgactat tccatagtat
    19381 gcttgaccag taaataaatg aaatgctaaa gcactaagaa gataagttaa actaataaat
    19441 aaaagagata agccaaaact aactagcagt ttatagatta taattaaagt acgattttgt
    19501 tgtttgacca caacattatt aatcgtacgg tagttatagt catcactaaa aaagatacgg
    19561 gctggtgtta aaaagaaaag cgtcacaaaa tcagtaataa tattaatgat ttgtatcatg
    19621 atgacttggt taggtgtctt gcgaaaacta taggctacac caacagcaat gacaacaagt
    19681 agcatacaag aaaatagagc taaacgctct tggcgtatcc gaaataaatc tgctttaaga
    19741 taggaaatca taaaaacctc tttctaaatt aatggtgatg ctgcatcttt acctaacaaa
    19801 tcgaggtaat aatgttcaaa gctcacttga tgatgataaa tgtcaaaaat ctcaacaggc
    19861 agatctagaa caattttgat aagctccatt actgataaag ttggttttat tagtagcatc
    19921 tgaccatcag tctcaactct aagccccaat tctaaaagtc tattttttac cagctgatca
    19981 tcgcttgtat gaagggcaat ttgtactttt acttgagctt taagctctgc tttgtccata
    20041 ctcttaataa ctttgccttt atgcataatc acgtaacgat caacaactaa gtctaattct
    20101 gataaaatat gactggaaat caaaatcgta ataccatagc aatctcttag gttcaaaata
    20161 atctgacgca tttctttgat tcctgctgga tcaagaccat taatgggttc atccaagatt
    20221 aaaaattctg gaaaatccag tatcgaaaga ccaatagcca aacgttggcg catccctaaa
    20281 gaataatctt tcaccttttt ctttttgctc actttttcta acccaataag ttccaaaacc
    20341 tcatgtatgc gctcttttgc atcagctaat ctcaattgca acgctgcata gtaaagatta
    20401 tcatgagctg tcatattggg ataaagtgtc ggtgactcaa taattgatcc cattcgatag
    20461 ggttgtaact gtttgatttg ccctgaatct tttccaatta ggccagataa aatacgcatt
    20521 aacgttgtct ttccagcacc attttgaccc acaaggccgc aaatttctcc tttgtaaata
    20581 tcaaaagaga tatcacttaa aacctgattt ttaccatagg atttatttac ttgctcaata
    20641 cttagtaaca ttgattgctg tgtcattttc cgctcctttt ttgtacttat cgattaatac
    20701 aatatcttac acataataa
//
LOCUS       DATXQK010000026        20696 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.026, whole genome shotgun sequence.
ACCESSION   DATXQK010000026 DATXQK010000000
VERSION     DATXQK010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20696
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.026"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            122..1105
                     /gene="guaC"
                     /locus_tag="VC394_001092"
     CDS             122..1105
                     /gene="guaC"
                     /locus_tag="VC394_001092"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEP5994566.1"
                     /translation="MFNDIPVFDYEDIQLIPNKCIITSRSQADTSVTLGKYQFKLPVI
                     PANMQTIIDETIAERLAKEGYFYIMHRFDEDSRKPFIKRMHEQGLIASISVGVKACEY
                     EFVTSLKEDAPEFITIDIAHGHANSVIDMIKHIKTELPETFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMVMIGSLFAGHIESPGKTVEVDGETFKEYYGSASEYQKGEHKNVEGKKIL
                     LPTKGHLSDTLTEMQQDLQSSISYAGGKDLDSLRHVDYVIVKNSIWNGDSI"
     regulatory      1228..1323
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            1410..1991
                     /locus_tag="VC394_001093"
     CDS             1410..1991
                     /locus_tag="VC394_001093"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEP5994567.1"
                     /translation="MQLLEERILTDGNILGENILKVDNFLTHQVDYRLMKAIGKVFAQ
                     KYAEAGITKVVTIEASGIAPAVYAAEAMDVPMIFAKKHKNITMTEGILTAEVYSFTKQ
                     VTSTVSIAGKFLSKEDKVLIIDDFLANGQAAKGLIEIIGQAGAQVVGVGIVIEKSFQD
                     GRRLIEDMGIEVTSLARIKNFENGNLNFLEADA"
     gene            1991..3274
                     /locus_tag="VC394_001094"
     CDS             1991..3274
                     /locus_tag="VC394_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEP5994568.1"
                     /translation="MTHSTKQEHSHSQSAVLGLQHVLSMYAGSILVPIMIAGALGYSA
                     RELTYLISTDIFMCGVATFLQLKLTKHTGVGLPVVLGCAFQSVAPLSIIGAQQGSGAM
                     FGALIASGIYVILVAGIFSKIARFFPPIVTGSVITVIGLSLVGVAMGNMGDNVKEPTA
                     QSMMLSLLTIVIILLVQKFTKGFVKSISILIGLVVGTLVSAMMGLVDTTPVVEASWIH
                     VLTPFYFGMPTFEITSIVMMCIIATVSMVESTGVYLALSDLTNDQLDEKRLRNGYRSE
                     GIAVFLGGLFNTFPYTGFSQNVGLVQISGIKTRRPIYYAAGILVVIGLLPKFGAMAQM
                     IPSPVLGGAMLVLFGMVALQGMQMLNRVDFQKNEYNFIIAAVSISAGLGFNGTNLFAS
                     LPETAQMFLTNGIVIATLTSVVLNLVLNGKDKQDE"
     gene            complement(3338..4276)
                     /locus_tag="VC394_001095"
     CDS             complement(3338..4276)
                     /locus_tag="VC394_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEP5994569.1"
                     /translation="MFVTQQLKLMGTVIDIQIESDKACQQLSRVIDLLYTYKNRFSAN
                     DSNSELMAINQAAGVKPLSVHSDLFNLIQIGKAHSLSTPSNLNIAIGPLVQAWRIGFE
                     DARVPSHNLISQQLALTDPRQVLIDDKKQTVFLQQVGMALDLGALAKGYITDKIMAYL
                     IEDGIDSALINLGGNVRVHGPNPKSPDKTFRIGIQKPDAKRGQHLGVIKVNNHSVVTS
                     GIYERQFTAKGKQYHHILDRQTGYPIETDMLSLTIMAPSSFYCDIWATRLFGLDSSMI
                     ITLLNTFDNIEGLLVTRKHHVLMSNGLRHYFQPYYH"
     gene            complement(4329..4514)
                     /locus_tag="VC394_001096"
     CDS             complement(4329..4514)
                     /locus_tag="VC394_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEP5994570.1"
                     /translation="MPFVTIDLFEGRSQEQKNQLAREVTEVVSRIAKAPKENIHVFIN
                     DMPEGTYYPQGEMKQKS"
     gene            4652..5221
                     /locus_tag="VC394_001097"
     CDS             4652..5221
                     /locus_tag="VC394_001097"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEP5994571.1"
                     /translation="MAQLYYKYGTMNSGKTIEILKVAHNYEEQGKPVVIMTSALDTRD
                     GFGIVSSRIGMRREAIPISNDMDIFTFIAQLEEKPYCVLIDESQFLSKQNVYDLARVV
                     DELNVPVMAFGLKNDFQNNLFEGSKHLLLLADKIDEIKTICQYCSKKATMVLRTENGK
                     PVYEGDQIQIGGNETYIPVCRKHYFNPEI"
     gene            5256..6335
                     /gene="prfA"
                     /locus_tag="VC394_001098"
     CDS             5256..6335
                     /gene="prfA"
                     /locus_tag="VC394_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEP5994572.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSREEANTR
                     ETVTAYREYKQVIQTISDAEEMIKDASGDPELEEMAKEELKESKAAKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAETQGWRFEVMESSVNGVGG
                     IKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRMVHIPTGIKVEMQEERTQQKNRDKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTVGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKMDEVIDALVMYDQTKKLESLNN"
     gene            6335..7174
                     /gene="prmC"
                     /locus_tag="VC394_001099"
     CDS             6335..7174
                     /gene="prmC"
                     /locus_tag="VC394_001099"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEP5994573.1"
                     /translation="MNYATLIRTYEDKLEQIDEDRENLSYVFREIKEWSSLDMLIHQN
                     QAVTPEDAVLLEHIFCSLSQHLSPQYITGNAYFRDLKLAVDKRVLIPRPETEELVDMI
                     LAENLDAPLNVLDIGTGSGAIAISLKKERPNWQVTASDISRAALDLAKANADAYQLDI
                     TFIESDVFSLISGTFDIIVSNPPYISYEDKEEVSLNVLQSEPHLALFAKENGYAIYRK
                     IIEQADNYLTKEGKLYFEIGYKQAEGIKDMLQAYFPQRHIRVVTDIFGKERMVVVDYG
                     VSS"
     gene            7158..7748
                     /locus_tag="VC394_001100"
     CDS             7158..7748
                     /locus_tag="VC394_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEP5994574.1"
                     /translation="MEYLASIIESGDALVLPTETVYGLFAKALDEKAVNAVYDLKQRP
                     RDKAMNLNVADFNSILAFSKEQPRYLKKLYQAFLPGPLTIILKANDQVPYWINSGLST
                     VGFRLPSHPITTALIQKTGPLIGPSANLSGKASGRVFDHIMQDFDFQVFGYADDPFLT
                     GKDSTILDLSGERAVILRQGAITKEELLANVPELRF"
     gene            7766..8218
                     /locus_tag="VC394_001101"
     CDS             7766..8218
                     /locus_tag="VC394_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP5994575.1"
                     /translation="MLRPLSKTDCPMLQEINAKALGYLVSLDLLERQYERLIEDCHHY
                     FLAYADKDTNQLLGYVHAERYETLYASDGLNLLGLAVLPAYQRRGIGSALLRALESQA
                     RQEGIAFIRLNSASHRKEAHAFYRNLDYADDKTQLRFIKNLQGDDNDF"
     gene            8208..9464
                     /locus_tag="VC394_001102"
     CDS             8208..9464
                     /locus_tag="VC394_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEP5994576.1"
                     /translation="MIFDKGNVEDFDKELWDAIHAEEERQEHHIELIASENMVSKAVM
                     AAQGSVLTNKYAEGYPGNRYYGGTECVDIVETLAIERAKKLFGAAFANVQAHSGSQAN
                     AAAYMALIEAGDTVLGMDLAAGGHLTHGSPVNFSGKTYHFVGYSVDADTEMLNYEAIL
                     EQAKAVQPKLIVAGASAYSRSIDFEKFRAIADHVGAYLMVDMAHIAGLVAAGVHPSPV
                     HYAHIVTSTTHKTLRGPRGGLILTNDEALAKKINSAVFPGLQGGPLEHVIAAKAVAFK
                     EALDPAFKDYAQAIIDNTAAMAAVFAQDDRFRLISGGTDNHVFLVDVTKVIANGKLAQ
                     NLLDEVNITLNKNAIPFETLSPFKTSGIRIGCAAITSRGMGVKESQTIAHLIIKALVN
                     HDQETILEEVRQEVRQLTDAFPLYKK"
     gene            9471..10448
                     /locus_tag="VC394_001103"
     CDS             9471..10448
                     /locus_tag="VC394_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEP5994577.1"
                     /translation="MLDSYIKRIVIHQFSPNDTELLLSDRLVSITPRIDEYFRKKLAK
                     VFSDEAKRGQFEANNTFFTTIGDDLLETSVTIAQLWKEAFVISEDQKTNDLVFVQFDK
                     DGEPFFAFLRIALKEQFAHLSDNYEHPFTVTQNNLPSPTQTPDEALVINLKSGQYYLI
                     EKRVKHNGSFANYFSEHLLKVTPEQSVKKSIKMIEQTAQKIAEHFNQDDFTFQSKMKS
                     TLFKQLEADDVLSPEKLADQLFDDNLTARLTFVDQVKDVIPEPIKISDIDHSRQIKKL
                     ENQKLSLSNGIELTVPNAIYQDAEAVEFLLNDDGTYSILIKNIEDIKTK"
     gene            10449..11047
                     /locus_tag="VC394_001104"
                     /pseudo
     CDS             10449..11047
                     /locus_tag="VC394_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407575.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            11057..12775
                     /locus_tag="VC394_001105"
     CDS             11057..12775
                     /locus_tag="VC394_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5994578.1"
                     /translation="MKRLRPYLKGYLKESILGPLFKLLEALFELLVPLLIANMIDISI
                     SQHNSQGILRVVLTLFGLATIGLLLSVTAQYFSSKAAVGFTRQMTDDLFKKIMFLSKE
                     DQDHLGYASLVSRLTSDSFQIQTGINQFLRLFLRAPIIVCGAMVMAYWISPSLTLWFV
                     MMVIVLLTLVFVMSHLLGPLYLLIRRETDHLVRLTSQQLQGIRVIKAFNQTQKELQAF
                     KQQNMLLSRHQYQAATLANVLNPMTFLVVNLTLLILIWQGSWQVAHRSLSQGMLVALI
                     NYLLQILAELLKMTMLMGTINQSVTAAKRINQVFVLADEVPLPLLKDGPISTHLLTIR
                     HLTFTYPGAAEPSLYDIQLSADQGEWIGIIGGTGAGKTTLIDLICQTYSQYSGEISLN
                     WQGEVPKTLTEWRNVIALVPQKAQLFKGTIRSNLLLGQSMPINDEELWRALELAQAKE
                     FVAALPEQLEAPVEAFGRHFSGGQRQRLAIARALLKPKPILILDDASSALDNETRGRL
                     FKALKEELSDVLVILVTQSIKNLQFADKILVLEQGHQLDFASHDQLKVSNALYQEMLA
                     LYVKGE"
     gene            12778..14505
                     /locus_tag="VC394_001106"
     CDS             12778..14505
                     /locus_tag="VC394_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5994579.1"
                     /translation="MIKTDHHLLKRVLQDLLKKPLPVCILVIASFVQVGLSVYLPVLI
                     GKAVDMSLSVNSWQTLKWLLGQMLVVIVVNTLIQWVMPLVYSRLLYQYSQQLKDKLLE
                     KIHRLPFAYLDRQTIGDLVSRVITDTEQLINGLQLVFNQFILGLLTILCTIIAMAQID
                     WLMLILVLVLIPSSLFLARFIAQKSFHYAQAQTKSRGNLAQFTEEVLRQEGLVQLFNA
                     QEQSICDYHVLNKTYCEASQKAIFYASTVNPATRFINSVIYALLAGLGAVRIMAGLFS
                     VGQLTTFLNVVVQYTKPFNDISSVLAEIQSSLACAQRLYDLLDIEIKEQEHFLTFKAS
                     AVKGQIDFEEVSFSYQKDRPLLKDINFSVPAGSKVAIVGPTGAGKSTLINLLMRFYEL
                     DAGSIKLDKVPIKCYAKEELRSITGMVLQETWLKDATVHELIAYGSEEASRGEVVAAA
                     KAAHAHFFIMQLPKTYDTYLSASDDALSQGQLQLLAIARMFLKKPKVLILDEATSSID
                     IRTEAVIQEALKELMRGRTSFIIAHRLSTIQSADLILVMDQGRLVEWGTHASLMSKNG
                     CYARLQKIE"
     gene            14746..16116
                     /locus_tag="VC394_001107"
     CDS             14746..16116
                     /locus_tag="VC394_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEP5994580.1"
                     /translation="MSKIVVVGANHAGTACIKTMLTNYGDANEIVVFDQNSNISFLGC
                     GMALWIGEQIAGPEGLFYSDKEELESLGAKVYMESPVQSIDYDTKTVTALVDGKNHVE
                     TYDKLIFATGSQPILPPIKGAEIKEGSLEFEATLENLQFVKLYQNSADVIAKLENKDI
                     KRVAVVGAGYIGVELAEAFQRKGKEVVLIDVVDTCLAGYYDRDLTDLMAKNMEEHGIQ
                     LAFGETVKEVAGNGKVEKIITDKNEYDVDMVILAVGFRPNTTLGNGKIDLFRNGAFLV
                     NKRQETSIPGVYAIGDCATIYDNATRDTNYIALASNAVRTGIVAAHNACGTDLEGIGV
                     QGSNGISIYGLHMVSTGLTLEKAKRLGFDAAVTEYTDNQKPEFIEHGNFPVTIKIVYD
                     KDSRRILGAQMAAREDMSMGIHMFSLAIQEGVTIEKLALTDIFFLPHFNKPYNYITMA
                     ALGAKD"
     gene            complement(16275..17258)
                     /locus_tag="VC394_001108"
     CDS             complement(16275..17258)
                     /locus_tag="VC394_001108"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEP5994581.1"
                     /translation="MTATKQHKKVILVGDGAVGSSYAFALVTQNIAQELGIIDIFKEK
                     TQGDAEDLSHALAFTSPKKIYAADYSDCHDADLVVLTAGAPQKPGETRLDLVEKNLRI
                     NKEVVTQIVASGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQAL
                     AAKIGVDARSVHAYIMGEHGDSEFAVWSHANVAGVGLYDWLQANRDIDEQGLVDLFIS
                     VRDAAYSIINKKGATFYGIAVALARITKAILDDENAVLPLSVFQEGQYEGVEDCYIGQ
                     PAIVGAYGIVRPVNIPLNDAELQKMQASANQLKAIIDEAFAKEEFASAAKN"
     gene            17449..19935
                     /gene="gyrA"
                     /locus_tag="VC394_001109"
     CDS             17449..19935
                     /gene="gyrA"
                     /locus_tag="VC394_001109"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEP5994582.1"
                     /translation="MQDRNLIDVNLTSEMKTSFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRHMLVD
                     GHGNFGSMDGDGAAAQRYTEARMSKIALELLRDINKNTVNFQDNYDGSEREPVVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLAESIDAVKMVMEHPDCTTRELMEVIPGPDFPTG
                     ALVMGRSGIHRAYDTGKGSIVLRSRTEIETTQTGRERIVVTEFPYGVNKTKVHEHIVR
                     LAQEKRLEGITAVRDESSREGVRFVIEIRREASATVILNNLFKLTSLQTNFSFNMLAI
                     ENGVPKILSLRQIIDNYISHQKEVIIRRTRFDKDKAEARAHILEGLLIALDHLDEVIA
                     IIRNSETDVIAQTELMSRFDLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LSDILAKPERIITIIKEEMDEIKRKYANPRRTELMVGEVLSLEDEDLIEEEDVLITLS
                     NKGYIKRLAQDEFRAQKRGGRGVQGTGVNNDDFVRELVSTSTHDTLLFFTNFGRVYRL
                     KAYEIPEYGRTAKGLPIVNLLKLEDGETIQTIINARKEETAGKSFFFTTKQGIVKRTE
                     VSEFNNIRQNGLRALKLKEGDQLINVLLTSGQDDIIIGTHSGYSVRFNEASIRNMGRS
                     ATGVRGVKLREDDRVVGASRIQDNQEVLVITENGFGKRTSATDYPTKGRGGKGIKTAN
                     ITPKNGQLAGLVTVDGTEDIMVITNKGVIIRTNVANISQTGRATLGVKIMKLDADAKI
                     VTFTLVQPEDSSIAEINTDRENSISKNKDN"
     gene            19955..>20696
                     /locus_tag="VC394_001110"
     CDS             19955..>20696
                     /locus_tag="VC394_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HEP5994583.1"
                     /translation="MVKKQKRRKIKSMSWARKLLIAVLLILGLALLFNKPIRNTLIAR
                     NSNKYQVTKVSKKQIKKNKEAKSTFDFQAVEPVSTESVLQAQMAAQQLPVIGGIAIPE
                     LGINLPIFKGLGNTELIYGAGTMKEEQVMGGENNYSLASHHIFGITGSSQMLFSPLER
                     AQNGMSIYLTDKEKIYEYIIKDVFTVAPERVDVIDDTAGLKEVTLVTCTDIEATERII
                     VKGELKTEYDFDKAPADVLKAFNHSYNQV"
BASE COUNT         6400 a         3651 c         4294 g         6351 t
ORIGIN      
        1 tttacctaaa tatacctcta atcatcagag gtcataggaa gattattttg tatagaggac
       61 ttagagaaga aggtgagaac tgacttgttt ttcttaatct atgttaaaag aggagaaact
      121 tatgtttaat gacatccctg tatttgatta tgaagatatt caactgattc ctaacaagtg
      181 cattattact agtcgttcac aagcagatac aagtgtgaca ctcggaaaat accagttcaa
      241 gctaccagtt atcccagcaa atatgcaaac cattattgat gaaacaattg ctgagcggtt
      301 ggcaaaagag ggttattttt atattatgca tcgttttgat gaagatagcc gcaaaccttt
      361 tatcaaacgc atgcatgaac aaggcttgat tgcatcaatc tctgttgggg ttaaagcttg
      421 tgaatatgaa tttgtgacct ctttgaaaga ggatgctcct gagtttatca ccattgatat
      481 tgctcatggt catgctaaca gtgttattga tatgattaag catatcaaga ctgaattacc
      541 agaaacattt gtcattgctg gaaatgttgg tacgccagaa gctgtacgag aactcgaaaa
      601 tgcaggtgct gatgctacta aagtaggcat cggtccaggc aaagtatgta tcactaaagt
      661 taaaaccggt ttcggtacag gtggttggca gctagcagca ctgcgctggt gcgcaaaggc
      721 agctcgtaaa cctatcattg ctgatggtgg gattcgcaca catggtgata ttgctaaatc
      781 catccgcttt ggagctagta tggtcatgat tggatcgctt tttgcaggac atattgaaag
      841 tcctggaaaa acggtagaag tggatggtga aacgtttaaa gaatattatg gctcggcgtc
      901 agaatatcaa aaaggcgagc ataaaaatgt agaaggcaaa aaaatcttat tgcctacaaa
      961 aggtcactta tcagataccc tgaccgaaat gcaacaggat ttgcaatcgt ccatttctta
     1021 cgcgggtggt aaagatttag acagtttgcg tcatgttgat tatgttattg tcaaaaattc
     1081 catttggaat ggtgattcca tttaaatgat atggagttag ctaggaccag taaagaattt
     1141 tttagcttaa tgctggagat gcttttatca tatctctgta ggtattgaaa aaaataagaa
     1201 acatggtaaa atagtaaatt atcataatga attcaataat gacatactta tttatgctgt
     1261 gaattggcgc agcgtctcta caagacacct taatgtctaa caataagtaa gcttttaggc
     1321 ttgcttggtt tttaaccttg caaagtgaga aactcttata gtttctcact ttgcggcagg
     1381 ctttttctat ttgaatcaaa aggagattta tgcagttact tgaagaacgc atcttaacag
     1441 atggcaatat tctaggagag aatattctaa aggtagataa ttttttaact catcaagttg
     1501 attaccggtt gatgaaagca attggtaaag tgtttgctca aaaatatgct gaggctggca
     1561 ttacaaaagt ggttacaatc gaagcttcag gtattgcacc agccgtatac gctgcagaag
     1621 caatggatgt tcctatgatt tttgcgaaaa aacataaaaa cattaccatg acagaaggca
     1681 ttttgacagc agaagtttat tctttcacta aacaagtgac aagcacggtg tctatcgctg
     1741 gtaaattcct atctaaagaa gacaaggttt tgattattga tgacttttta gctaatggtc
     1801 aggcagccaa aggcttgatc gagattattg gtcaagcagg ggcacaagtc gtcggcgttg
     1861 gtattgtgat tgagaaatct ttccaagatg gtcgtcgatt gattgaagat atgggcattg
     1921 aagtgacctc attagcacgt attaaaaact ttgaaaatgg taatctaaac ttcttggagg
     1981 ctgacgcata atgacacatt caacaaagca ggaacattct cactcgcaat cagctgtcct
     2041 tggcttacag catgtgcttt cgatgtatgc cggatctatt ttagtaccaa ttatgatcgc
     2101 aggtgcttta ggttattccg ctagagagtt gacctacctg atttcgacag atattttcat
     2161 gtgtggtgtg gctactttct tacagttaaa gttaaccaag catacgggag tgggattacc
     2221 agttgtttta ggatgtgctt ttcaatcagt ggcacctcta tccattattg gagcgcaaca
     2281 aggttcaggt gccatgtttg gagccctcat tgcgtcagga atttatgtca ttttagtggc
     2341 aggtattttt tctaagattg ctcgtttctt cccaccaatt gttacaggat cggtcattac
     2401 cgttattggc ttaagtctcg taggtgttgc tatggggaat atgggagata acgtcaaaga
     2461 gcctacggca caaagcatga tgttatcatt gttgacgatt gtcattattc ttttagtcca
     2521 aaaatttacc aaaggttttg tgaaatccat ttccattttg attgggcttg tagtgggaac
     2581 tttggtttca gccatgatgg gattagtaga tacaactcct gttgtagagg cttcttggat
     2641 tcatgtgcta accccatttt attttggcat gccaactttt gaaatcactt ctattgtgat
     2701 gatgtgtatc atcgcaacgg tttctatggt tgaatcaaca ggtgtctact tagcgctttc
     2761 agatttgact aatgatcaat tagatgaaaa acgtttgcgc aatggttatc gctcagaagg
     2821 gatcgcagtc tttctcggtg ggttatttaa taccttccct tacactggtt tttctcaaaa
     2881 cgttggactt gtccaaatct caggtattaa aacccgtcgt ccgatttact acgcagcagg
     2941 tatccttgtg gtcattggcc tactccctaa atttggagca atggcacaaa tgattccaag
     3001 cccagttctt ggtggagcta tgcttgttct ctttggtatg gttgctcttc aaggaatgca
     3061 aatgcttaat cgtgttgatt tccaaaagaa cgagtataat tttattattg ctgccgtttc
     3121 catctcagca ggtttaggct ttaatggcac taatcttttt gccagcttac cagagacagc
     3181 tcaaatgttc ttaacgaatg gtattgtgat tgccactttg acatctgttg tcttaaattt
     3241 agttttaaat ggcaaagaca aacaagatga ataattaaaa aacggctaat tgagccgttt
     3301 tttctgtgat cagttttgag caaaccgtta acccctttta atgatagtat ggttgaaaat
     3361 agtgtctcaa accatttgac ataaggacat ggtgctttct tgttactaga agaccttcaa
     3421 tattatcaaa ggtatttaaa agtgtgataa tcattgagct atcaagacca aataaacggg
     3481 tagcccatat gtcacaataa aaagaagaag gagccatgat cgttaagcta agcatatctg
     3541 tttctatagg atagcctgtc tgacgatcta agatatgatg atactgtttc cctttggctg
     3601 taaactgcct ttcataaatt ccagaagtca caacagagtg attgttaacc ttgatgacac
     3661 caagatgttg cccacgttta gcatctggtt tttgaatgcc aattcgaaaa gtcttgtcag
     3721 ggcttttcgg attaggtcca tgaacgcgta catttccacc taaattaata agggcagagt
     3781 caataccatc ttcaatcagg taggccataa tcttatcagt aatataacct tttgctaagg
     3841 ctcctaagtc taaagccatt cctacttgtt gtaaaaagac agtctgtttt ttgtcatcta
     3901 taagaacctg tctaggatct gttagagcta actgttgtga gataaggtta tgactaggca
     3961 ctctagcatc ttcaaatcca attcgccagg cttgaacaag tggcccaatg gcgatattaa
     4021 gattacttgg tgttgacaag ctatgagctt taccaatttg gattaggtta aataaatcag
     4081 agtgaacgct gaggggttta acaccagccg cctgattaat agccatcaat tcagagtttg
     4141 aatcattagc tgaaaaacga tttttgtaag tatataataa gtcaatgact ctacttagtt
     4201 gttggcaagc tttgtcagat tcgatctgaa tatcaataac tgttcccatt agctttaatt
     4261 gttgcgtaac aaacataatt agtcctcttt ttagcagaat acccccacta aggagtgggg
     4321 gaaggtcatt atgatttttg tttcatttca ccttgaggat agtaagttcc ttcaggcata
     4381 tcgttgataa aaacatggat attttcttta ggtgcttttg caatgcgtga gactacttcg
     4441 gtaacctcgc gagctaattg atttttttgt tcttgtgagc gtccctcaaa caagtcaatt
     4501 gttacaaatg gcataatgtt actcctttat gttattgggc atcattgtaa cataatgctg
     4561 tgaaaaagta tacactgttt tcaatggctg ggaaaacttc agcctttgtg gtaaaatagg
     4621 tagaatacta tagaaagtag gattttccgc tttggctcaa ctctattata aatatgggac
     4681 aatgaattct ggtaaaacca ttgaaatttt aaaagttgct cataactatg aagaacaagg
     4741 caaacctgtt gtcattatga caagtgctct tgatacacgt gatggttttg gcatcgtctc
     4801 aagccgtatt ggtatgcgac gagaagctat ccccatttca aatgacatgg atattttcac
     4861 atttattgcc caattggagg aaaaacctta ttgtgtcctg attgacgaaa gccaattttt
     4921 aagcaagcaa aatgtttatg atttggcgcg tgtggtggat gagctaaatg ttcctgtcat
     4981 ggcttttggt ttgaaaaatg atttccaaaa caatcttttt gaaggttcaa aacatttgct
     5041 attattggcc gataagattg atgaaatcaa aacaatttgc caatactgta gtaagaaagc
     5101 aaccatggtt ttaaggacag aaaacggaaa acctgtctat gaaggtgatc agatacaaat
     5161 tggtggtaat gaaacatata tcccagtttg tcgaaaacat tattttaatc cagagattta
     5221 aaaggtaatc ggttttaaag gagatacaaa gaaaaatgaa tatttatgat caattgcagg
     5281 cagttgaaga tcgttatgaa gagttaggtg agttacttag tgatccagat gttgtttcag
     5341 atacaaagcg atttatggaa ttatcacgtg aagaagctaa tactcgagaa actgtcacag
     5401 cttatagaga atataagcaa gtcattcaaa ccatttcaga tgctgaagaa atgatcaaag
     5461 atgccagtgg tgatcctgaa ttagaagaaa tggctaaaga agaacttaaa gaatcaaaag
     5521 cagctaaaga agagtatgaa gaaaaattaa aaatccttct attacctaaa gatcctaacg
     5581 atgataaaaa cattatttta gaaattcgtg gtgctgctgg tggcgatgaa gcagcgcttt
     5641 ttgctggtga tcttttaaca atgtatcaaa aatatgctga aactcaagga tggcgttttg
     5701 aagttatgga atcatccgtt aatggtgttg gtggtattaa agaagtggtt gccatggttt
     5761 ctggtcagtc agtttattct aagcttaaat atgaatcagg agctcatcgt gttcaacgtg
     5821 tcccagtgac agagagtcag ggtcgtgttc atacttcaac tgcaactgtt ttggtaatgc
     5881 cagaagttga agaagtagaa tacgacattg atcctaaaga ccttcgcgtc gatatttacc
     5941 acgcgtctgg tgctggtggt cagaatgtca ataaagttgc tactgcagtt cgtatggtac
     6001 acattcctac aggaatcaaa gttgaaatgc aagaagagcg tactcagcaa aaaaaccgtg
     6061 ataaagcgat gaaaatcatt cgtgctcgtg ttgctgacca ttttgcgcaa attgctcaag
     6121 atgaacaaga tgctgaacgt aaatcaacag ttggtactgg tgatcgttca gaacgtattc
     6181 gtacttataa tttcccacaa aatcgtgtaa cagatcatcg tattggtttg acacttcaaa
     6241 aattagatac tattttatct ggaaaaatgg atgaagttat tgatgcactc gttatgtatg
     6301 atcagacaaa gaaattagaa tctctcaata actaatgaac tatgctacat taatcagaac
     6361 ttatgaagat aaactagaac aaatagatga ggatcgcgaa aatctctcct atgtttttag
     6421 agaaatcaaa gaatggtcaa gtctagatat gttgattcac caaaatcaag ctgtgactcc
     6481 tgaagatgct gttcttttgg aacatatttt ctgttctcta agtcagcatt tatctcctca
     6541 atatattaca ggtaatgctt attttcgtga tttgaagctt gcagtcgaca agcgtgtatt
     6601 aattccaagg ccagaaacag aagaattagt tgacatgatt ttagctgaaa atcttgatgc
     6661 accgttgaat gttttagata ttggtacagg tagtggagcg attgctattt ctctcaaaaa
     6721 agaaaggcct aactggcaag taacggcatc tgatatttct agagctgctc ttgacttggc
     6781 aaaagccaat gctgatgctt accagcttga tataacgttt attgaatcag atgtttttag
     6841 tctcatttca ggaacatttg atattattgt gtcaaatcct ccctacatat cctacgagga
     6901 taaggaagaa gtcagtctaa atgttttgca atcagagcct catttggcac tttttgctaa
     6961 agaaaatggc tatgctattt accgtaagat tattgaacaa gctgacaatt acttaactaa
     7021 ggaggggaag ctttactttg aaattggtta taagcaagct gaaggtataa aagatatgtt
     7081 acaagcctat tttcctcaaa gacatatacg agtcgtaacg gatatatttg gcaaagaaag
     7141 aatggtggtt gttgattatg gagtatctag ctagtatcat tgaatcaggt gatgcccttg
     7201 ttttgccaac agaaacggtt tatggtcttt ttgccaaggc tttagatgaa aaagcagtaa
     7261 atgctgttta tgacttaaaa caaagacccc gagataaagc aatgaacctt aatgtggctg
     7321 attttaatag tatcttagcc ttttcaaaag aacagccgag atacctcaag aaattatacc
     7381 aagccttttt accaggtccg ttaacgatta ttttaaaagc taatgaccag gttccttatt
     7441 ggattaattc gggtctttca acggttggtt ttcgcttacc cagtcatcca attaccacag
     7501 ctttaattca aaaaacaggc cctttaattg gtccttctgc aaacttatct ggcaaagcta
     7561 gtggtcgagt ttttgatcat attatgcaag attttgactt tcaagtgttt ggttatgcag
     7621 atgatccttt tctaacaggt aaggattcaa ccatcttaga cttatcaggt gagcgtgctg
     7681 tgattttacg tcaaggtgct atcaccaaag aagaattatt agccaacgtg cctgaacttc
     7741 gcttttaaga gaggagccgc ttggaatgtt gagaccatta agtaaaacgg actgtcctat
     7801 gctacaagaa attaacgcca aagcccttgg ctatttagtt tcgcttgact tgttagaaag
     7861 gcaatatgaa cgattgattg aggattgcca tcactatttt ttagcttatg ccgacaagga
     7921 taccaatcaa ttattaggct atgttcacgc tgaacggtat gagacccttt atgcttctga
     7981 tggtcttaat ctcttgggct tggctgtttt accagcctat cagagaaggg gaattgggag
     8041 tgccttacta agggccctgg aatcccaagc taggcaggaa ggcattgcct ttattcgcct
     8101 caattctgcc agtcatcgta aagaagcgca tgctttttac cgaaacctcg actatgcaga
     8161 tgataaaaca caattacgat tcatcaaaaa cttacaagga gatgacaatg atttttgata
     8221 aaggtaatgt tgaagacttt gataaagagc tttgggatgc cattcatgct gaagaagaaa
     8281 gacaagaaca tcatatcgaa ttgattgcat ctgaaaacat ggtttcaaaa gctgtcatgg
     8341 cagctcaagg ttctgtttta accaataagt atgcagaagg ctatccaggt aatcgctatt
     8401 atggtggaac agaatgtgtg gatattgtcg aaacgcttgc tattgaacgt gctaagaaac
     8461 ttttcggtgc cgcatttgcc aatgttcaag cccattccgg aagtcaagcc aatgcagcag
     8521 cttatatggc cttgattgag gctggtgaca ccgtattagg aatggatttg gctgcaggtg
     8581 gtcatctcac gcatggctct ccagtaaatt tttctggaaa aacctaccat tttgtgggtt
     8641 actcagtaga tgctgatact gaaatgttaa attatgaggc gattttagaa caagcaaagg
     8701 cggtccagcc aaagcttatc gttgcagggg cttcggccta ctctagaagc attgattttg
     8761 aaaagtttcg tgccattgct gatcacgtag gtgcttactt aatggtggac atggctcaca
     8821 tcgctggcct tgtagcagca ggtgtgcacc cgagtcctgt ccattatgcg catattgtga
     8881 catcaacaac ccataaaacc ttacgtggtc ctcgtggagg cttgatatta accaatgatg
     8941 aagctcttgc taaaaagatt aattcggccg ttttccctgg tttacaagga ggacctctgg
     9001 aacacgtgat tgctgccaaa gcggttgcct tcaaagaagc cttggatcca gcctttaaag
     9061 actatgcaca agctattatt gataatactg cggctatggc agcagttttt gcacaagatg
     9121 atcgtttccg tctgatctca ggtggtacag ataatcatgt ctttttagta gatgtcacta
     9181 aggttattgc taatggtaaa ctagcgcaaa atcttctgga tgaggttaat attactctaa
     9241 ataaaaatgc cattcctttt gaaaccttat ctccttttaa aacatcaggc attcgtattg
     9301 gttgtgcagc tataaccagt cgtggcatgg gagttaaaga aagccaaact atcgctcacc
     9361 tcatcatcaa agccttggtt aatcatgatc aagaaactat tcttgaagag gttcgtcagg
     9421 aagtgcgtca gttaaccgat gccttcccac tctataaaaa gtaaggaatc atgttagata
     9481 gttatataaa acgtattgtc attcatcaat tttctcccaa tgacactgag ttactgttat
     9541 cagatcgttt agtgagcatt acaccacgaa ttgatgaata tttccggaaa aaattggcta
     9601 aagtgttctc ggatgaagcc aaacggggtc agtttgaggc taataatacc ttttttacaa
     9661 ccattggtga tgatttgttg gaaacgtctg ttaccattgc ccagttatgg aaggaagcct
     9721 ttgttatttc agaggatcaa aaaaccaatg atttagtctt tgtccagttt gataaggatg
     9781 gcgaaccctt ttttgctttc ttgcgaattg ctttgaaaga acagtttgct cacctttctg
     9841 acaattatga acatccattt acggtgacgc agaataatct gccgagtccc acccagacac
     9901 cagatgaagc actagtcatt aatctcaaat ctggtcagta ctacttgatt gaaaagcgtg
     9961 tgaagcataa tggcagtttt gctaattatt tttcagaaca cctcttgaaa gttacacctg
    10021 agcaatctgt taaaaagtct atcaaaatga tagaacaaac cgctcaaaaa attgcagagc
    10081 attttaatca agatgatttt acttttcagt caaagatgaa atccactctt tttaagcagt
    10141 tagaagcaga cgacgtttta tcaccagaaa aattagctga ccaactcttt gatgataatt
    10201 taacagcccg tttaaccttt gtggatcaag tcaaagatgt cattccagaa cctataaaaa
    10261 ttagcgatat tgatcattct agacagataa aaaaactaga aaatcaaaag ctatcgttat
    10321 caaacgggat agaattaaca gtaccaaatg ccatttatca ggatgctgaa gcggttgaat
    10381 ttttgttaaa tgatgatggg acttattcaa tcttgattaa aaatatagag gacattaaaa
    10441 ccaaatagat gtttagactt ttaaagcgtg cctgcagttt tcttcttctt tttgtcattt
    10501 atcaatcctt tgttattcat cacaatgttc aacgtgtttt ggcatataag cctatggttg
    10561 agaaaacatt agcagaaaat gatacaaagg caaatgtaga cttagtctta gctatgatat
    10621 acaccgagac aaaaggagga gaagcagatg tcatgcaatc cagtgaaagc agttcaggac
    10681 aaaaaactcg attacagata gtcaggccag tattgagcac ggggtgaatt tactgtctca
    10741 taatcttgct ctagctgagg aagcaggagt agattcttgg acggctgttc aggcctataa
    10801 ttttggaaca gcttatattg attatattgc taaacatgga ggtcaaaata ccgttgattt
    10861 agcgactacc tattccaaaa ccgttgtggc gccaagccta ggcaacacta gtggtcaaac
    10921 ctatttttat taccaccccc tcgccttgat ttctggaggt aagctttata agaatggcgg
    10981 caatatctat tactcacgag aagttcattt caatctttat ttaattgaac tgatgagcct
    11041 tttttagaaa gttatcatga aacgattacg tccatatttg aaagggtacc taaaagaaag
    11101 tatcttaggt cctcttttta aattattaga agctttattt gaattattag tccctttgtt
    11161 aattgctaat atgattgata tatcgattag tcaacacaac agccagggaa ttttgagggt
    11221 tgttttaaca ttatttggtt tagcaaccat tggcttattg ctttccgtta cagcccagta
    11281 tttctcttcg aaagcagctg ttggttttac aagacaaatg acagatgatt tgtttaaaaa
    11341 aatcatgttt ttgagcaagg aggaccaaga ccatcttggt tatgctagtc tggtatcacg
    11401 attgaccagt gatagttttc aaattcaaac tggtatcaat caatttttgc gtctttttct
    11461 aagggcccct attatcgtat gtggtgctat ggtaatggct tattggatta gtccaagtct
    11521 aaccctatgg tttgtgatga tggtgattgt cttactaaca cttgtttttg ttatgtcaca
    11581 tttattaggt cccctttatc tcttaatccg ccgagaaaca gatcacttgg ttcgtttgac
    11641 tagccaacag ttacaaggaa tccgagtaat taaggctttt aatcaaaccc aaaaagaact
    11701 ccaagcattt aagcagcaaa atatgctctt aagtcgccac caatatcaag ctgctaccct
    11761 agccaatgtc ttgaatccta tgactttttt agtggtcaat cttactctgt taatcttgat
    11821 ttggcaagga agttggcaag tagctcatag atcgcttagt cagggcatgt tggttgcttt
    11881 aattaattac ctcttgcaga ttttggcaga acttttgaaa atgaccatgt tgatgggaac
    11941 catcaatcaa agtgtaacag ccgcaaaacg gattaatcag gtgtttgttt tagctgatga
    12001 agtgcctttg cccttactta aagatggtcc tatttcaact catctgttga ccatccggca
    12061 cttgaccttt acttatccag gagcagcaga gccaagtctg tatgatattc agttaagtgc
    12121 tgatcaggga gaatggattg gcatcattgg tggtactgga gcaggcaaaa cgactttgat
    12181 tgacttgatc tgccaaactt attcacaata tagtggtgag attagtctta attggcaagg
    12241 agaagtgcca aaaacactaa ccgagtggcg aaatgtgatt gctcttgttc ctcaaaaagc
    12301 tcaattattt aaaggaacga ttagaagtaa cctcctactg ggacaatcaa tgcctattaa
    12361 tgatgaggag ctgtggcggg ctcttgaatt agctcaagca aaagagtttg tcgctgcctt
    12421 accagaacaa ttagaggcac ctgttgaagc ctttggccgt catttttcag gagggcaacg
    12481 gcaacgtcta gctattgcca gagcacttct gaagccaaaa cccattttga ttttggatga
    12541 tgccagttcc gccttagaca atgagactcg aggacgactt tttaaagctc taaaagaaga
    12601 gttatcagat gttttagtga ttctagtcac gcaatccatt aagaatttgc agtttgctga
    12661 taagatttta gtgttggaac agggccatca attggatttt gccagtcatg accagttaaa
    12721 agtaagcaat gcactttatc aagaaatgct agcgctttac gtgaaggggg agtaaaaatg
    12781 attaaaacag accatcacct cctaaagcga gtgcttcagg atttgttaaa aaaacctcta
    12841 cctgtttgta ttctcgtgat agctagtttt gtacaagttg gtttaagtgt ttacttgcct
    12901 gttcttatcg gtaaagccgt tgatatgagt ttgtcagtca attcttggca aactttaaaa
    12961 tggctgttgg ggcaaatgtt agttgttatt gtggtcaaca cattgattca atgggtgatg
    13021 cctcttgtct atagtcgttt gctatatcaa tatagccagc aattgaaaga taagcttttg
    13081 gaaaaaattc atcgtcttcc ttttgcatat ctagatcgtc aaaccattgg agatttggtt
    13141 agtcgagtga taaccgacac tgagcaactg atcaatggtc tacaattggt ttttaaccag
    13201 tttatacttg gactattgac gattctgtgt accattatag ctatggcaca gattgactgg
    13261 ctcatgttaa tcttggtttt ggtgttaata ccaagctccc tctttttagc tcgttttatt
    13321 gctcaaaaga gttttcatta tgcacaagca cagaccaaaa gcagaggaaa tttagctcag
    13381 tttactgaag aagtccttcg tcaagaaggt ttggttcaac tgtttaatgc tcaagagcag
    13441 tctatctgtg attatcatgt tttaaataaa acctattgcg aggcttcaca aaaagccatt
    13501 ttttacgcct caacagtcaa tccagccact cgctttatta atagtgtcat ttatgcttta
    13561 ctggcaggtt taggggctgt tcgtatcatg gctggacttt tttcagtagg tcagctaacc
    13621 acttttttaa atgttgttgt ccaatatacc aaacctttca acgatatttc atctgtcttg
    13681 gcagagatac agagttcttt agcttgtgcg caaaggcttt atgacctttt agatattgaa
    13741 ataaaagagc aagagcactt tctaacattt aaggcatcag cggttaaggg acaaattgac
    13801 tttgaagaag taagtttttc ttatcaaaaa gacaggccct tactgaagga tattaatttt
    13861 tctgttcctg cgggttccaa ggtagcgatt gtagggccta caggtgcagg caaatcgact
    13921 ttaatcaatt tactcatgcg cttttatgaa cttgatgctg gtagcatcaa gttagataaa
    13981 gttcctatta agtgttatgc taaggaagaa cttaggtcca ttactggcat ggtattgcaa
    14041 gaaacctggt tgaaagatgc gactgttcat gagttgattg cttacggcag tgaagaggct
    14101 agccgtggtg aagtagtggc agcagccaaa gcagctcatg cacacttctt tattatgcaa
    14161 cttcctaaga cttatgatac ttacttaagt gcttctgatg atgctttgtc ccaagggcag
    14221 ctccagttat tagctattgc cagaatgttt ttgaaaaaac caaaagtctt gattctagat
    14281 gaagccacct cctctattga tattagaaca gaagctgtta ttcaagaggc actaaaagaa
    14341 ctcatgagag gaaggaccag ctttatcatt gcccatcgtt tatcaacgat tcaatcagct
    14401 gatttgattc ttgttatgga tcaaggtcga ttggttgagt ggggaacaca tgccagctta
    14461 atgtcaaaaa acggatgtta tgctagatta caaaagatag aataaagttc gtcttttaac
    14521 aaaggtgaac tttttaagtc ttcctaaagt tagttaatta acaaacttta aactgttata
    14581 taaaaaaata acagcgaaaa agcggacgat ctttttttat aagtgagaaa aaacagttga
    14641 ctaaagaaga aataactcaa aaaagtgagc aatatatttg gaagcgtttt ttgactgtgg
    14701 tataatagag ccctatataa tattgtgaaa tgaggaacaa aatatatgag taaaatcgtt
    14761 gttgttggtg caaaccatgc tggtacggcc tgtatcaaga ctatgttaac aaactacggt
    14821 gatgctaatg agattgttgt atttgaccaa aactcaaata tttcattttt aggctgtggt
    14881 atggcacttt ggattggtga gcaaattgct ggaccagaag gacttttcta ttcagataaa
    14941 gaagaattag agtctttagg ggctaaagtt tacatggaat cacctgttca atcaatcgac
    15001 tacgatacca aaacagtgac agcgcttgtt gatggtaaaa accacgtgga aacttacgac
    15061 aagttgattt ttgcaactgg ctcacaacct atcttaccac cgattaaagg tgccgaaatc
    15121 aaagaaggat cacttgaatt tgaagcaact cttgaaaatc ttcaatttgt gaagttatac
    15181 caaaactcag ctgatgtgat tgcaaagctt gaaaacaaag acattaaacg tgtagccgta
    15241 gttggtgctg gttacattgg tgttgagtta gctgaagctt tccaacgcaa aggcaaagaa
    15301 gtggttctga ttgatgtcgt ggacacttgc ttggcaggtt attacgaccg tgatttgact
    15361 gacttaatgg ctaaaaacat ggaagaacat ggtattcaac tagcctttgg tgaaacagtt
    15421 aaagaagtag ctggtaatgg taaggttgaa aagatcatta ctgacaaaaa tgagtacgat
    15481 gtggatatgg ttatcctcgc cgttggtttc cgtccaaaca caactcttgg aaatggtaag
    15541 attgatcttt tccgtaatgg tgctttcctt gtcaataaac gccaagaaac ttctattcca
    15601 ggtgtttatg ctattggtga ctgtgcaact atctacgata atgctactcg cgatacaaac
    15661 tacattgctt tagcttcaaa tgctgtccgt acaggaattg tagcagcaca taacgcttgt
    15721 ggtacagacc ttgaaggtat tggcgttcaa ggttcaaacg gtatttccat ttacggcttg
    15781 cacatggtat caactggttt gactcttgaa aaagctaaac gacttggttt tgatgctgca
    15841 gtgactgagt atactgataa tcaaaaacct gaatttatcg aacacggtaa cttcccagta
    15901 accattaaga ttgtttacga taaagactca cgtcgtatct tgggtgctca aatggcagcc
    15961 cgtgaagata tgtcaatggg aattcacatg ttttcacttg ctatccaaga aggagtgacc
    16021 attgaaaagt tggcattaac tgatattttc ttcttaccac atttcaacaa accttacaac
    16081 tacatcacaa tggcagcact tggtgccaaa gactagtatc tgataggaaa aagccagagc
    16141 agttcagcag cactggcttt tttgcttttt aattgttgca acctgaaaag ttctaaactt
    16201 atgattatcc tcttatagca cgttggaaaa ggaatatcag gcaaaacaaa aagagaccga
    16261 agtctctttt gtgattagtt tttagcagca gaagcaaatt cttccttagc aaaggcttca
    16321 tcaatgatag ctttcaattg atttgcagag gcttgcattt tttgcaattc agcatcgtta
    16381 agtggaatgt ttactggacg aacaattcca tacgctccaa cgatagctgg ttgaccaata
    16441 tagcaatctt caacaccttc gtattggcct tcttggaaaa cagaaagcgg aagaacagca
    16501 ttttcatcat caagaatagc ttttgtaata cgagctaggg caactgcaat accgtagaaa
    16561 gtagcacctt ttttattgat gattgagtaa gcagcatcac gtacagaaat gaaaagatca
    16621 actaggcctt gctcatcaat gtcacgatta gcttggagcc agtcgtaaag acctacacca
    16681 gcaacgttag cgtgtgacca aacagcaaat tcagagtcac cgtgttctcc catgatgtaa
    16741 gcgtgaaccg aacgcgcatc aacaccgatt ttagcagcca gagcttgacg gaaacgggct
    16801 gaatctagag atgtacctga accaatgaca cgctctttag ggaaacctga gaatttccat
    16861 gttgagtaag tcaaaacgtc aactgggtta gcagcaacaa gaaagattcc tttgaaacca
    16921 gatgcaacga tttgtgtaac aacctcttta ttgatacgaa ggtttttttc aaccaggtca
    16981 aggcgtgttt cacctggttt ttgaggagca ccagctgtta agacaacaag atcagcatca
    17041 tgacagtctg agtagtcagc tgcatagatt tttttaggtg atgtaaaagc tagagcgtgg
    17101 cttaaatctt ctgcatctcc ttgagttttt tctttaaaaa tatcaataat accaagttct
    17161 tgagcaatat tttgtgttac caatgcaaaa gcgtatgatg aaccgacggc accatcaccg
    17221 accaagatta cttttttgtg ttgtttagtt gcagtcattt ctaaacatct ccttaatttt
    17281 attagagcaa aagctcatac ggtcttattt taacatattg ataattgttt gtcattgaat
    17341 tcagaaaaat aatgaaaata gtttactgat aacgcttcca ataagaaaaa ataaggaatt
    17401 tatggtataa tgaaatctag tattgactag aaaaggagca ttccttaaat gcaagatcga
    17461 aatttaattg acgtcaatct gacaagtgaa atgaaaacga gtttcattga ctatgccatg
    17521 agtgtcattg tggcaagagc tttgccagat gtgcgtgatg gtctgaaacc tgtgcatcgt
    17581 cgtattctct atgggatgaa tgaactcggt gtgacacctg ataaaccgca taaaaaatct
    17641 gctcgtatta cgggtgatgt catgggtaaa tatcacccgc atggggattc ttctatttat
    17701 gaagctatgg ttcgtatggc tcagtggtgg agttatcgcc atatgcttgt tgatggccat
    17761 ggcaattttg gttctatgga tggtgatggt gctgccgctc aacgttatac tgaagcgcgc
    17821 atgagtaaaa ttgcgcttga attattaagg gatatcaata aaaataccgt taattttcaa
    17881 gataactacg atggaagcga aagagaacca gtagtattac cagctcgctt tcctaattta
    17941 ttagtcaatg gagcgacagg gattgccgtt gggatggcaa ctaacattcc gcctcataat
    18001 ttggcagagt ctattgatgc tgtcaagatg gtaatggaac atccagactg tacaacaaga
    18061 gagttgatgg aagtgatccc tggacctgac tttccgactg gtgcccttgt tatggggcgc
    18121 tcaggtattc atagggctta tgatactggt aaaggttcta tcgttttacg ctctcgaaca
    18181 gaaattgaaa caactcaaac aggtcgggaa cgtattgtgg ttactgaatt tccctatggc
    18241 gtcaataaaa cgaaagtgca tgaacacatt gttcgtttag cacaagaaaa aagactagaa
    18301 ggtattacag cagttcgtga cgaatctagc cgtgaaggag tgcgttttgt tatcgagatt
    18361 cgtcgagagg catccgcgac agttatctta aataacctct ttaaattaac aagccttcag
    18421 acgaatttca gttttaatat gttggctatt gaaaatggtg tgcctaaaat attgtcactc
    18481 agacaaatca ttgataatta cattagtcat caaaaagaag ttattatacg tcgcacccga
    18541 tttgataaag ataaagctga agccagagct catattttag aaggcttact gattgccctt
    18601 gatcatttag acgaagtgat tgctattatt cgtaacagtg agacagatgt cattgctcag
    18661 acagaattga tgtcacgttt cgacttgtct gaacgccaaa gccaagctat tttggatatg
    18721 cgtttgcgtc gtttgactgg tttggaacgt gataagattc aatcagaata tgatgatttg
    18781 ttggccttga ttgctgactt atctgatatt ttagccaaac cagaacgtat tatcactatc
    18841 atcaaagaag aaatggatga gatcaagcgt aaatacgcta acccacgccg tacagaatta
    18901 atggtcggtg aagtcctttc tctagaagat gaagacctaa ttgaagaaga agatgttttg
    18961 attaccttgt caaataaagg ttatatcaag cgtcttgctc aagatgaatt tcgtgcccag
    19021 aagcgtggtg gacgtggcgt ccaaggaact ggagtcaaca atgacgactt tgtccgtgaa
    19081 ttggtatcaa caagtaccca tgatactttg ctcttcttta cgaattttgg gcgtgtctat
    19141 cggttgaaag cttacgaaat tccagaatat ggtcgtacag caaaaggtct tccaattgtt
    19201 aatctattga aattagaaga tggtgaaacc attcaaacta ttatcaacgc acgtaaggaa
    19261 gaaacagcag gtaaatcctt cttctttaca acgaaacaag gcattgtcaa acgtacagaa
    19321 gtttcagaat ttaataatat ccgtcaaaac ggtctccgtg ctcttaagtt gaaagaggga
    19381 gatcagctga ttaacgtcct tttgacaagc ggtcaggatg atattatcat tggaactcac
    19441 tccggatatt ctgttcgttt taatgaagca agcatccgca acatgggccg atcagctacg
    19501 ggtgttcgcg gggttaagct tcgtgaagat gatcgcgttg ttggcgcgtc acgtattcaa
    19561 gataaccaag aagttcttgt aattaccgaa aatggctttg gtaagagaac aagtgctaca
    19621 gactatccta caaaaggtag ggggggaaaa ggaataaaaa cagctaatat tacccctaaa
    19681 aatggtcaat tagctggatt ggttaccgta gatggtacag aagatatcat ggtgattaca
    19741 aataaaggtg tcattattcg cacaaatgtt gcaaatattt cgcaaacagg tcgtgctact
    19801 ttgggtgtta aaattatgaa actagatgct gatgctaaga ttgtaacatt taccttggtt
    19861 cagcctgaag atagtagtat cgctgaaatc aatactgatc gtgaaaatag tatttcaaaa
    19921 aataaagata attaatcata acaaggagta aaaaatggta aaaaaacaaa agcgtcgaaa
    19981 aataaaatcc atgtcttggg caagaaaact actaatagct gtacttttaa ttctaggttt
    20041 agcattgctt tttaacaaac ctatccgaaa tacattaatt gctcgaaact ctaataaata
    20101 tcaagtaacg aaagtttcga aaaaacaaat caaaaagaat aaagaggcta agtcaacatt
    20161 tgatttccaa gccgtagagc cagttagtac agagagtgtc ttgcaagcac aaatggcggc
    20221 tcagcaactt cctgttatag ggggcattgc cataccagag cttggcatta atttaccaat
    20281 ttttaaaggt ttaggaaata ctgagcttat ttatggcgca ggaacgatga aagaagaaca
    20341 agttatggga ggagaaaata attattctct tgccagtcat catatttttg gaattacagg
    20401 ttcatctcaa atgctctttt cgccgcttga aagagcacaa aatgggatgt ccatctattt
    20461 aacagataaa gaaaaaattt acgaatacat cataaaagat gttttcacgg tagctcctga
    20521 acgcgttgat gttatcgatg atacagctgg tctcaaagaa gtgactttag tgacttgtac
    20581 agatatcgaa gcaacagaac gtattattgt caaaggagaa ctaaaaacag aatacgattt
    20641 tgataaagcg cccgccgatg tattgaaagc ttttaatcat tcttataacc aagtat
//
LOCUS       DATXQK010000027        20324 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.027, whole genome shotgun sequence.
ACCESSION   DATXQK010000027 DATXQK010000000
VERSION     DATXQK010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20324
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.027"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..795)
                     /locus_tag="VC394_001111"
     CDS             complement(<1..795)
                     /locus_tag="VC394_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEP5994584.1"
                     /translation="MVTIKDVAQKAGVNPSTVSRVLKDNRSISMKTKEKVRKAMADLG
                     YVPNVAAQILASGLTHNIGLVFPPITTSDRLSEPFFMEILSTITNEAKESHFTVSIAT
                     GISLDDLIEQVKLMHLQKRVDGFIILYSDQDDPVKKYLMTNNLPFVIVGAPQGDTNKT
                     TYIDNDNQLMAKTAVEHLYQKGHHNILFITDDLLSEVTSERYLGYLKGCFKRSLKTKP
                     MLLFDRKDPVSVEILMETISSFKATALVVVGDVLAIRMVQLLSFYDI"
     gene            1038..2285
                     /locus_tag="VC394_001112"
     CDS             1038..2285
                     /locus_tag="VC394_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEP5994585.1"
                     /translation="MKSWQKVIVGGASLTLASTLLVGCGSGSKDKKEAGADSKTIKLW
                     VPTGSKKSYADTIAKFEKDSGYTVKVVESEDPKAQEKIKKDASTAADVFSLPHDQLGQ
                     LVESGTIQEVPEKYNKEIAATSTDQALVGAQYKGKTYAFPFGIESQILFYNKSKLAAE
                     DVTSYDTITTKATFGGTFKQANTYATGPLFMSVGNTLFGENGEDVKGTNWGNEKGAAV
                     LKWIADQASNKGFVSLDANNVMSKFGDGSVASFESGPWDYEAAQKAIGKENLGVAIYP
                     KVTIGGETVQQKAFLGVKLYAVNQAPAKGDTKRIAASYKLASYLTNAESQENQFKTRN
                     IVPANKEVQSSEAVQSNELAKTVITMGSSSDYTVVMPKLSQMGTFWTESAAILSDAFN
                     GKIKESDYLTKLQQFDKDIAATK"
     gene            2559..3920
                     /locus_tag="VC394_001113"
     CDS             2559..3920
                     /locus_tag="VC394_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEP5994586.1"
                     /translation="MTQSNLTPNISVIEALKRGSWDIKLSSIIMGFANFANKQFIKGI
                     LFLISELIFLVAFVSQIIPAIRGLVTLGTQTQGMTTKTIDGINIQVAVDGDNSMLMLI
                     FGLASLIFCLVFAYIYWCNLKSARNLYLFKQKGQKIPNFKEDLATLTNGRFHMTLMAI
                     PLIGVLLFTILPLIYMICLAFTNFDHNHLPPKSLFDWVGLANFGNVLSGRMAGTFFPI
                     FSWTLIWAVFATVTNFFFGIILALLINAKGLKWKKMWRTIFVITIAVPQFISLLIMRN
                     LLNDEGPLNALLNKIGLINGSLPFLSDPLWAKFSIIFVNMWIGIPFTMLIATGIIMNL
                     PSEQIEAAEIDGASKFQVFKSITFPQILLIMTPNLIQQFIGNINNFNVIYLLTGGGPT
                     NSEYYQAGTTDLLVTWLYKLTVTAADYNLASVIGILIFTVSAIFSLLAYTRTASYKEG
                     AAK"
     gene            3920..4756
                     /locus_tag="VC394_001114"
     CDS             3920..4756
                     /locus_tag="VC394_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEP5994587.1"
                     /translation="MKNKRRFQLGLVYATLIILSIIWLFPIAWVILTSFRSEGTAYVN
                     YFIPKTFTLNHYINLFTNETFPFGKWFMNTLIVATFTCIISTFITVAIAYSLSRIKFK
                     FRNGFLKLALILNMFPGFMSMIAIYYILKALGLTQTLTALVLVYSSGAALGFYIAKGF
                     FDTIPYSLDESAMIDGATRMDIFFKITLPLAKPIIVYTALLAFMGPWIDFIFAQVILG
                     DATSKYTVAIGLFSMLQPDTINNWFMAFTAGSVLIAVPITLLFMFMQKYYVEGITGGS
                     VK"
     gene            4914..5711
                     /locus_tag="VC394_001115"
     CDS             4914..5711
                     /locus_tag="VC394_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEP5994588.1"
                     /translation="MTSKITIKDIAREAGVSVATVSYVLNQRNDQKISEETRNKVLHI
                     SNLLNYTPNQAARALVTQQSNSVVLYYKYADSILARAEQLHTISFLSRLLHEQDYQLV
                     CLNEDSIEKFDWADLILTLDVDNDTFYKIGKLNFKPLIAIDSFVNDPLFFQINTDYAA
                     LKDQANQFFKDEPYTLLSLPITNRERYEHVSAFFPSLHIVNDLRELTYFEKENLLLID
                     ESLDQFFVNSSQKLYCPRFSPKKGQVILEAIEWAKKRQPDKKHNLLV"
     gene            complement(5827..6642)
                     /locus_tag="VC394_001116"
     CDS             complement(5827..6642)
                     /locus_tag="VC394_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEP5994589.1"
                     /translation="MPDKKQFPISYISHCLSARAMFASRAQFTWWQNLLIIVFLNALI
                     MIPVTLHYANMTTYPLERIVTKSLSPITDKTYQALTQGKIEKDTFQGQSLIRRDGELV
                     LAVLPTKVDLEQLASESTRQIIVTKKEWRFVTPDGKELRAHVRGQQQSLADLTTVKAV
                     KDFVNQQWYDSNKASVLGFLLLTFVLMVCVGTLIVIGLGAFFLTLTKRSRLFMIRNFS
                     EGLGLMVNCLAWPSLLAIALSFFIQDPVLIMNCQVFGTLLMLTWVFYKTQFRD"
     gene            complement(6664..7524)
                     /locus_tag="VC394_001117"
     CDS             complement(6664..7524)
                     /locus_tag="VC394_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEP5994590.1"
                     /translation="MRKTYVSISMKRKRFFTQLLTYLYLISLVIVILFPILVTVSSAF
                     RPGNTTAFSFHFDGPWTLSNFKTLFQDTLYLRWYWNTLIVAFFTMLIQVTVITLTGYA
                     YSRYNFFGRKKSLIFFLVVQMVPTMAALTAYFVMAWLFNALNQYWFLILIYVGGGIPM
                     NAWLMKGYFDTVPYDLDESAKLDGSGHFRTFYQIVLPLVRPMIAVQSLWAFMGPFGDF
                     MLAKFLLRAQENYTVAVGLQSFITNDARNPKVTLFAAGAILIAVPISVLFFFLQKNFV
                     SGLTSGGTKG"
     gene            complement(7521..8828)
                     /locus_tag="VC394_001118"
     CDS             complement(7521..8828)
                     /locus_tag="VC394_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEP5994591.1"
                     /translation="MVDNQRQQKPNKAMWLSVIPGLGQLYNKQIVKGGVLLVVFFLEL
                     LEIVVLGIPALTGFYSLGSVPMQDHSLFMLIKGAMQLITLVLFGIFHLVAMRDAKLVA
                     HQMNEGKKVPVTAKETLEAIYEKGFPYLLIIPAYLAMAFAIIFPVLVTLLIAFTNYDF
                     RHIPPYRLLDWVGLKNFLNILELSTFKTAFTSVLSWTLIWTLAATSLQIVIGIATAVI
                     ANQSFIRGKRLFGVIFLLPWAVPAFISIMSFGNFFNDSIGAMNVQVLPFIEKFLPFVD
                     FGIVSWKTDPFWTKVAVIMVQGWLGFPYIYILVSGILQAIPADLYEAATVDGATVLQK
                     FRHITMPMIFAVAAPTFISQYTFNFNNFSIIYLFNNGGPGTVGGGAGTTDILISWIYK
                     LTTQTSPQFSMASAVTLIISVIVISVSLITFKKFKAFDMEERS"
     gene            complement(8903..11038)
                     /locus_tag="VC394_001119"
     CDS             complement(8903..11038)
                     /locus_tag="VC394_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclomaltodextrin glucanotransferase"
                     /protein_id="HEP5994592.1"
                     /translation="MRELHIKTYKLLTKSAVLLGLISFPLTVSAADNASVTNKADFST
                     DTIYQIVTDRFNDGNTSNNGKTDVFDKNDLKKYHGGDWQGIIAKIKDGYLTDMGISAI
                     WISSPVENIDSIDPSNGSAAYHGYWAKDFFKTNQHFGTEADFQQLVKVAHQHHIKVVI
                     DFAPNHTSTAEKEGTTFKEDGALYKNGKLVGKFSDDKDKIFNHESWTDFSTYENSIYH
                     SMYGLADLNNINPKVDQYMKEAIDKWLDLGVDGIRVDAVKHMSQGWQKNWLSHIYEKH
                     NVFVFGEWFSGHTDDDYDMTTFANNSGMGLLDFRFANAIRQLYTGFSTFTMRDFYKVL
                     ENRDQVTNEVTDQVTFIDNHDMERFATKVANNQTAVNQAYALLLTSRGVPNIYYGTEQ
                     YATGDKDPNNRGDMPSFNKESQAYKVISKLAPLRKQNQALAYGTTEQRWISDHVLVFE
                     RKFGNHVALVAINRDQTNGYTITNAKTALPQNSYKDKLEGLLGGQELIVGADGTISSF
                     ELGAGQVAVWTYEGEDKTPQLGDVDASVGIAGNKITISGQGFGNSKGQVTFGEISAEI
                     LSWSDTLITLKVPTVPANYYNISVTTADKQTSNSYQAFEVLTDKQIPVRLLINDFKTV
                     PGEQLYLMGDVFEMGANDAKNAVGPLFNNTQTIAKYPNWFFDTHLPINKEIAVKLVKK
                     DSIGNVLWTSPETYSIKTGHEAQTITIKK"
     gene            complement(11064..12767)
                     /locus_tag="VC394_001120"
     CDS             complement(11064..12767)
                     /locus_tag="VC394_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HEP5994593.1"
                     /translation="MNVAGMLHIPDSRYCFAISQKELVIRLRVAKEDRGITVNLVHGP
                     KYGYHEFQEELEMSVAYTDHTHAYYETTLTLDDVRLAYIFKIEEEGQTYYFSEDGLTT
                     SYDFSNGFYNFFQMPYINAVDVHQVIPWTKDAVFYQIFVDRFNRGDWEKGDSYVNMGW
                     LEKPTPKSFAGGDLKGITEKLDYLKDLGITVIYLTPIFQSISNHKYDISDYYAIDPQF
                     GTKYDLQELIDLAHQMGIKIILDAVFNHASSDAVEFQDVLRYGKESKFFDWFMTHDEH
                     PSMDLVNYETFAGCNYMPKWNTSNRDVQDYLIEIGRYWIKEFCIDGWRLDVSDEVSHD
                     FWRRFRQAVKAEKADAILIGENWHDAYPYLAGDQYDGIMNYAFTKACLDYFAFETMTS
                     KQLAEKLSNILMRNTWQVNRMNLNLLDSHDTHRFFTQVKGSKDKLLAALALLFIFMGI
                     PCLYYGTEVAMEGGYDPDSRRGFPWDDAERDKDFFSKVKAIIALRQQPALQEGAISID
                     YDEDCLILKRSLGEQSISLVLNNGESAYSIKEGGQILTSNGVTKNKKQLLQDGYIVFS
                     N"
     gene            complement(12933..14192)
                     /locus_tag="VC394_001121"
     CDS             complement(12933..14192)
                     /locus_tag="VC394_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEP5994594.1"
                     /translation="MSWNWKKASVLGTLSLASVLPLTACVSGGGKGVKETDGKTIVVS
                     VDEGYVDYIKSIKGEFEKEHKVTVKVKKEGMMDTLDKLSTDGPTGASPDVFLAPFDRV
                     GGLGTEGQISEVTLGNSKEFDDTVKKLVTIDGKTYGAPDVIETLVTYYNKDLVPQAPK
                     SFTELEVLQKDSKFAFASEPGKSVGFLAKWTDFYYGYGLIAGYGGYIFGDKGTKPSDL
                     GLGNDGTVEGLNYAKQWYGTWPQGMQDTKKAGDFITEQFISKKAGVIIDGPWAASSFK
                     DAGVNFGVMEIPTLTNGKKYQPFAGGKAWVISNYSKGKTTAQKFLDYVTNAENQKRFY
                     DKTQEIPANLTARNYASKEGNELTKAVISQFESAQPMPNIPEMAEVWEPGANMFFNVA
                     SGKEEASKAAKEAAKTIKEAIEQKYAE"
     gene            complement(14801..15784)
                     /locus_tag="VC394_001122"
     CDS             complement(14801..15784)
                     /locus_tag="VC394_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEP5994595.1"
                     /translation="MVIIAVVIFFGVILGILIVEYGQKRSFSSWLVEKMFAYRKYKLS
                     KKVDNYKGVETQRNRNIPYHLDNPEEIVGFSTQEYDVNGIQVFIINDQKQSHQPVIYY
                     LYGSVYLRRPEKLHFKTVKHLIQLTNAKVVFPNFPKVPYATYQDVYPKLVEAYQQFCL
                     SNGPSQVSVIGDSSGGGMALAFAKYIRDTKTALQPKQLILISPWLDVSFKNQAYKDFE
                     AEEAYLDARRLRDIGELWAGGQGNMQLPYVSPLYGDCEDLPEITTFVGTKEIFYPDIM
                     SLHDQLTRQRIANQLIIAEKMIHAYVIFPIREAKQARKQIAELVLKTVEPT"
     gene            complement(15815..17065)
                     /gene="dltD"
                     /locus_tag="VC394_001123"
     CDS             complement(15815..17065)
                     /gene="dltD"
                     /locus_tag="VC394_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEP5994596.1"
                     /translation="MLKRLWLILGPLLIAFVLVVITIFSFPTQLDHSIAQEKANAVAI
                     TDSSFKNGLIKRQALSDETCRFVPFFGSSEWSRMDSMHPSVLAERYKRSYRPFLIGKR
                     GSASLSHYYGMQQITNEMQKKKAIFVVSPQWFTAQGINPSAVQMYLSNTQVIEFLLKA
                     RTDKESQFAAKRLLELNPGVSKSNLLKKVSKGKSLSRLDRAILKCQHQVALREESLFS
                     FLGKSTNYEKRILPRVKGLPKVFSYKQLNALATKRGQLATTNNRFGIKNTFYRKRIAP
                     KYNLYKNFQVNYSYLASPEYNDFQLLLSEFAKRKTDVLFVITPVNKAWADYTGLNQDK
                     YQAAVRKIKFQLKSQGFHRIADFSKDGGESYFMQDTIHLGWNGWLAFDKKVQPFLETK
                     QPVPNYKMNPYFYSKIWANRKDLQ"
     gene            complement(17058..17297)
                     /gene="dltC"
                     /locus_tag="VC394_001124"
     CDS             complement(17058..17297)
                     /gene="dltC"
                     /locus_tag="VC394_001124"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEP5994597.1"
                     /translation="MSIEETVIELFDRLFMEDVSEMMDEDLFDAGVLDSLGTVELIVE
                     LESTFNIKVPISEFGRDDWNTVTKIVQGVEELQHA"
     gene            complement(17315..18571)
                     /gene="dltB"
                     /locus_tag="VC394_001125"
     CDS             complement(17315..18571)
                     /gene="dltB"
                     /locus_tag="VC394_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEP5994598.1"
                     /translation="MMMFFSHIPYMEPYGNPIYFVYLILAFLPVIIGIFKQKRLSTYE
                     TLVSLVFILFMFGGDHYQQLVAFLFYLLWQIISVFAYQKYREKANSAGVFYLAIAMAL
                     FPLIWVKVAPLTGPSSQTLFSFLGISYLTFKSIGMIIEMRDGTLQEVRLPDFIRFMIF
                     FPTFSSGPIDRFRRFQEDYHKLPERDDYFAMLNKAVMYLMLGFLYKHIISYCLGGILL
                     PLLENKALMVGGYFNKETILVMYVYGLNLFFDFAGYSMFAIGISYLLGIRTPENFNMP
                     FLSASLKDFWNRWHMSLSFWFRDYVFMRLVHLLIKHKTFKNRNVTSGVAYLVNMLVMG
                     FWHGLTWYYIAYGLFHGIGLIINDAWIRKKKEINRHRKKKGLSPLFQSRAFHVLCIVV
                     TFHVVMFSLLLFSGFLNDLWFNRPLH"
     gene            complement(18568..20106)
                     /gene="dltA"
                     /locus_tag="VC394_001126"
     CDS             complement(18568..20106)
                     /gene="dltA"
                     /locus_tag="VC394_001126"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEP5994599.1"
                     /translation="MIKDMIDSIEQFAQTQADFPVYDCLGEHRTYGQLKRDSDSIAAF
                     IDSLALLAKSPVLVFGAQTYDMLATFVALTKSGHAYIPVDVHSAPERILAIIEIAKPS
                     LIIAIEEFPLTIEGISLVSLSEIESAKLAEMPYERTHSVKGDDNYYIIFTSGTTGQPK
                     GVQISHDNLLSFTNWMIEDAAFDVPKQPQMLAQPPYSFDLSVMYWAPTLALGGTLFAL
                     PKELVADFKQLFTTIAQLPVGIWTSTPSFADMAMLSDDFCQAKMPALTHFYFDGEELT
                     VSTARKLFERFPSAKIINAYGPTEATVALSAIEITREMVDNYTRLPIGYPKPDSPTYI
                     IDEDGKELASGEQGEIIVTGPAVSKGYLNNPEKTAEAFFTFKGQPAYHTGDIGSLTED
                     NILLYGGRLDFQIKYAGYRIELEDVSQQLNQSPMVASAVAVPRYNKEHKVQNLLAYIV
                     VKDGVKERFDRELELTKAIKASVKDHMMSYMMPSKFLYRDSLPLTPNGKIDIKTLINE
                     VNNR"
     gene            complement(20118..20261)
                     /locus_tag="VC394_001127"
     CDS             complement(20118..20261)
                     /locus_tag="VC394_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEP5994600.1"
                     /translation="MIKNKSRMRGIAVFIGKTILFYLILMLLVYFFGYLGHGQSNFIY
                     NEF"
BASE COUNT         6509 a         4142 c         3402 g         6271 t
ORIGIN      
        1 tatatcataa aaagataata actgtaccat acgaatagcc agaacatcac caacaacaac
       61 caaggcagtt gctttaaagg aagatatagt ttccatcaat atttctacgc taacaggatc
      121 cttacgatca aataacagca ttggcttcgt ttttaaagat cgcttaaaac aacccttcaa
      181 atagccaaga taacgctcag aagttacttc tgatagtaaa tcatctgtta taaataatat
      241 gttgtgatgg cctttttgat aaaggtgttc tactgctgtt ttagccatta gctgattatc
      301 attatcaata taagtggttt tattagtgtc accttgtgga gcacctacaa tgacaaatgg
      361 taagttattt gtcatcaaat attttttgac aggatcatct tgatcagaat aaagaataat
      421 aaaaccatca actcgttttt gaagatgcat cagtttcact tgctctatca ggtcatcaag
      481 agatatacct gtagcaatcg acacagtaaa atgagattct ttagcttcgt tagtaatagt
      541 cgacaaaatt tccataaaga aaggttcact taatctatcg ctagttgtaa taggaggaaa
      601 aacaagtcct atattgtgtg tcaaaccact cgctagaatc tgtgctgcga catttggaac
      661 atatcccaaa tctgccatcg cttttctaac cttctctttt gttttcatag aaatagatcg
      721 attatctttt aataccctac tcaccgtcga agggttaaca cctgcctttt gagcgacatc
      781 ttttattgtt accatattaa cctcgttact atatttacca aagataaacc ttatcatggg
      841 tttgtcatgt atttcgtttc cattatacaa ttatctttca aaaaatcaac ttcaatcaag
      901 attcccttgt cattttaact caaattctct aaactatttt taaaaaaaac gacctttttt
      961 taaaaaaagg cttgactttt tctgcaagcg gttgcataat attaagtgta agaaatatac
     1021 cttaggagga aatagctatg aaatcatggc aaaaagttat cgtcggcgga gcaagtttga
     1081 cacttgcaag taccttgtta gtgggatgtg gatcaggttc aaaagataaa aaggaagctg
     1141 gcgctgactc aaagactatt aaactttggg taccgactgg ctctaaaaaa tcatatgcag
     1201 ataccattgc aaaatttgaa aaagattctg gttacaccgt taaagtggtt gaatcagaag
     1261 atccaaaagc acaagaaaaa atcaaaaaag atgcctcaac tgctgccgat gtcttttcac
     1321 ttcctcatga tcaacttggt caacttgtag aatctggtac tattcaagaa gttccagaaa
     1381 agtacaataa agaaattgct gctacttcta ctgatcaggc gctcgtcggt gcacagtaca
     1441 aagggaaaac atatgctttc ccatttggta ttgaatcaca aattcttttc tacaataaat
     1501 caaaattagc tgctgaagac gttacttcat atgatacaat tactacaaaa gcaacttttg
     1561 gtggtacctt taaacaagct aacacttacg ctactggtcc attattcatg tcagttggta
     1621 acactttatt tggggaaaac ggtgaagatg taaaaggtac taactggggc aatgaaaaag
     1681 gcgctgctgt tcttaaatgg atcgcagacc aagcttcaaa taaaggtttt gttagcttag
     1741 atgctaacaa cgttatgtct aaatttggtg acggttcagt agcttctttt gaatcaggac
     1801 cgtgggacta tgaagctgct caaaaagcta tcggtaaaga aaaccttggt gttgctattt
     1861 atccaaaagt aactattgga ggcgaaacgg ttcaacaaaa agcattcttg ggtgtaaaac
     1921 tttacgcagt caaccaagca ccagctaaag gtgatacaaa acgtatcgca gctagctaca
     1981 aattggcatc ctacttgact aatgctgaaa gccaagaaaa ccaattcaag actcgtaaca
     2041 ttgtcccagc caacaaagaa gttcaatctt cagaagctgt tcaatcaaac gaacttgcta
     2101 aaaccgttat cactatgggt tcttcttcag actacactgt cgtaatgcca aaacttagcc
     2161 aaatgggcac attctggact gaaagtgctg ctattcttag tgatgccttc aacggtaaaa
     2221 tcaaagaaag cgactacctt actaagttgc aacaattcga caaagatatc gctgcaacaa
     2281 aataatatga tctcatgtca tcactgctat actaaaacaa gcacaaggta agtacttcta
     2341 aaaagttctt tccacttacc ttatgaagag tggaactagg ctccatcaca ttttattcaa
     2401 gcttagtctc actcttactt gtttttgttt tactagtagc ttagtgctac ttaaattttt
     2461 aactctttaa aagtttacgt aacacttagc tatttcatcc taacaagaga ttgaatctga
     2521 actaccactc tagtatccta aaataaagga gaatctatat gactcagtca aacttgacac
     2581 caaatatatc tgtaatagaa gctttaaaaa gaggcagttg ggatattaaa ctttcgagta
     2641 ttatcatggg atttgccaac tttgctaaca aacagtttat caaaggaatc ttatttttaa
     2701 taagtgaact tatctttcta gtagcttttg tctcccaaat catccctgct atccgtggcc
     2761 ttgtcactct tggtactcaa actcagggaa tgactacaaa aactattgat gggattaaca
     2821 tccaagttgc tgttgatggc gataactcta tgctaatgct tatttttggt ttagcatcac
     2881 tcattttctg cctagtcttt gcttacattt actggtgtaa tctcaaaagt gctcgtaacc
     2941 tttatctttt caaacaaaaa ggtcagaaga taccaaactt caaagaagat cttgccacac
     3001 ttacaaatgg tcgctttcat atgactttaa tggctatccc actgattggt gtgcttttat
     3061 tcaccattct tcctttgatt tacatgatct gtttggcctt taccaatttt gatcataatc
     3121 acttgcctcc aaaatcttta tttgattggg tgggacttgc taactttgga aatgttttaa
     3181 gcggccgtat ggcaggaacc ttttttccta tcttctcatg gactttgatt tgggctgttt
     3241 ttgccacagt aactaatttc ttctttggta ttattcttgc tttgttaatc aatgccaaag
     3301 gattaaagtg gaaaaaaatg tggcgaacca tctttgttat caccattgca gttccacaat
     3361 tcatctcatt attgattatg cggaacttac tcaatgacga aggcccacta aatgctcttc
     3421 tcaataaaat cggcttgatt aatggctcac tgccattttt atctgatcct ctttgggcaa
     3481 aattctcaat catttttgtc aacatgtgga tcggtattcc atttaccatg ttgattgcga
     3541 ctggtattat catgaacctt ccaagtgaac aaattgaggc tgctgaaatt gatggcgcta
     3601 gcaaattcca agtcttcaag tctatcacat tccctcaaat tctcttgatc atgacaccaa
     3661 acttgattca acaatttatc ggaaatatca ataactttaa cgtcatttac ctccttactg
     3721 gtggtggtcc aacaaattca gaatactatc aggcaggaac aacagacttg ctggtcactt
     3781 ggctctataa attaaccgtc acagctgctg actacaattt agcttctgtt atcggtatct
     3841 taatctttac agtttcagct atctttagct tacttgctta tacaaggaca gcatcctaca
     3901 aggaaggagc ggctaaataa tgaaaaacaa acgtcgattt caacttggac ttgtttatgc
     3961 tactttaatc atcctctcaa tcatttggct cttcccaatc gcttgggtta ttcttactag
     4021 cttccgtagc gaaggaactg cttacgtcaa ttattttatt cctaagactt ttacattaaa
     4081 tcactacatt aatcttttta ctaatgaaac ctttcctttt ggaaaatggt ttatgaatac
     4141 tcttattgta gccactttta catgtattat ttcaaccttc ataactgttg ccatagccta
     4201 ctctcttagc cgaattaagt ttaagtttcg caatggcttt ttaaaactag cccttatttt
     4261 aaatatgttc cctggtttca tgagcatgat tgccatctac tatatcttaa aggccttagg
     4321 tttgacgcaa accctaacag cacttgtcct tgtttattcc tcaggtgcag cccttggttt
     4381 ttacattgca aaaggatttt ttgataccat tccttattca cttgatgaat cagctatgat
     4441 tgatggcgct actcgtatgg atattttctt taaaataacc cttcctttag caaaaccaat
     4501 cattgtctat actgcccttc ttgcttttat ggggccttgg attgacttta tctttgcaca
     4561 agttatctta ggtgatgcta caagtaaata taccgttgct attggactat tttcaatgct
     4621 tcagccagat accattaaca actggtttat ggccttcact gctggttctg tcttaattgc
     4681 cgtcccaatc actctcctct tcatgtttat gcaaaaatac tatgttgaag gcatcacagg
     4741 tggttctgtt aaataagtta caacatacca aaccttctaa gggcaagccc tttaggaggt
     4801 ttttgataaa taaggccgta gataatcata ttagtcctca catttatctt cagatcagcc
     4861 aaatgtatta atgattcctt gacttttacc atgaattcgg atataacaaa atgatgacat
     4921 ctaaaattac cattaaagat attgcacgag aagccggggt ttctgtggcg accgtatctt
     4981 acgtgttaaa ccagcgcaat gatcaaaaaa tcagcgaaga aacccgcaat aaagtgctac
     5041 atattagtaa ccttcttaac tatacaccta atcaggcagc cagggcatta gtcacacaac
     5101 aatcaaacag tgttgtcctg tattacaaat atgcagatag cattttagct agggctgaac
     5161 aactgcatac cattagcttt ctatcccgcc tattgcatga gcaggactat cagctggttt
     5221 gcctaaatga agatagcatt gaaaaatttg actgggcaga ccttattctg accttggacg
     5281 ttgataatga taccttttac aaaattggaa agcttaattt caaaccctta attgccattg
     5341 atagctttgt taatgatccc ctcttctttc aaattaatac agattatgct gctctcaaag
     5401 atcaggctaa tcagtttttt aaggatgaac cttataccct gctttccttg cctatcacta
     5461 atcgtgagcg atatgagcat gtttctgcct tctttccaag tttgcacatt gtaaacgacc
     5521 taagagagct aacctacttt gaaaaggaaa atcttttact cattgatgag agtttggatc
     5581 aattctttgt gaactcttcg caaaaactct actgcccacg tttttcccca aaaaaagggc
     5641 aagttatctt agaggccatc gaatgggcca aaaaacgcca gcctgataaa aaacataacc
     5701 ttctggtgta acgcctgaga attaacctat cttgactaat tccttgtaac tcaaaacctt
     5761 gcctctttag ataaagaagc aaggtttttt gatgtctaat ctaataacag ggcctgactt
     5821 gtctcatcaa tctctaaact gtgttttgta gaacacccag gtcagcatta ataaggtacc
     5881 aaatacttgg caattcataa tcagtactgg atcctgaata aagaaactaa gagcaatcgc
     5941 cagtagactc ggccaagcca aacaattaac catcaatcca agtccttcag agaagtttcg
     6001 aatcataaat agccgtgatc gtttagtcag agtcaaaaag aaagcgccta gaccaatcac
     6061 aataagggta cccacacaca ccatgagaac aaacgtcagc aaaagaaaac caagtacact
     6121 ggctttatta ctgtcatacc attgctgatt gacaaagtcc ttcactgctt taactgttgt
     6181 caggtcagcg agtgactgct gctggcctct aacgtgagca cggagttcct tgccatcagg
     6241 ggtaacaaaa cgccattctt tttttgtcac aataatttgt ctggttgatt ctgatgccaa
     6301 ttgctctaaa tcgacctttg tcggtaaaac agccaaaacc aattcaccat ctcgtcgaat
     6361 cagagattga ccctgaaacg tatctttctc aatctttcct tgggtcaatg cttgataggt
     6421 tttgtctgtg ataggcgaca aactcttagt tacaatccgt tccaagggat acgttgtcat
     6481 gttagcatag tgtaacgtaa ctggaatcat tattaaggcg ttcaaaaaaa caataataag
     6541 aagattttgc caccaagtaa actgagctcg gctggcaaac attgccctag cagaaaggca
     6601 atgtgaaatg taagaaatag gaaattgttt tttatctggc attaaacatt actccttgtc
     6661 aggttaccct ttggtaccac cacttgttaa accggaaacg aaatttttct gtaggaagaa
     6721 aaagagaact gaaataggca ctgcaatcag aatagctccc gcagcaaata aggttacctt
     6781 gggatttcta gcatcattcg taataaacga ttgaagacca actgctaccg tataattttc
     6841 ttgcgccctt aaaaggaatt tagccaacat gaagtctccg aaaggaccca taaaagccca
     6901 taaggattgt actgcaatca ttggtcgcac aagaggaagg acaatttggt agaaggtcct
     6961 aaagtgtcca gaaccatcta acttagcaga ttcgtccaag tcataaggta ccgtgtcaaa
     7021 ataacccttc atcaaccagg cattcatagg aataccacca ccgacgtaaa tcaaaatcaa
     7081 gaaccagtat tggtttaagg cattaaagag ccacgccata acaaagtaag ccgttaaagc
     7141 cgccatggta ggaaccatct gtacgactag gaaaaagatt aaactttttt tgcgtccaaa
     7201 gaaattgtaa cggctatacg cataacctgt caaagtgatg actgttactt gtatgagcat
     7261 ggtgaagaat gccacgatca aggtattcca ataccatctt aagtataagg tatcttgaaa
     7321 gagtgtttta aagttagaca aggtccatgg cccatcaaaa tgaaaactaa aggctgtggt
     7381 atttcctggt ctaaaagctg aactgaccgt cactagtatt gggaataaaa tcacgatcac
     7441 taaggagatt agatagagat aagtcaataa ttgggtgaag aaacgtttgc gtttcatgga
     7501 tattgaaaca taggtctttc tcatgatctc tcctccatat caaaagcttt aaatttctta
     7561 aaggtaatca acgataccga aatcactatt acagagatga ttaaggtcac tgccgacgcc
     7621 atcgaaaact gaggcgaggt ttgtgtggtc aacttgtaaa tccaagaaat cagaatatca
     7681 gttgtcccag ctccgccccc cacagtacca gggcccccat tattaaacag ataaataata
     7741 gagaaattat tgaagttaaa ggtgtactga gagataaagg ttggtgctgc aaccgcaaaa
     7801 atcattggca tggtgatatg gcggaatttc tgtaagactg tcgcaccatc aacggttgct
     7861 gcttcgtata aatcagctgg aattgcctgt aaaatacctg ataccaaaat atagatatag
     7921 ggaaatccta gccaaccttg caccataata acagccactt ttgtccagaa ggggtctgtc
     7981 ttccaggaca caattccgaa gtcaacgaaa ggaaggaatt tctcgatgaa aggaagaacc
     8041 tgaacattca tggccccaat ggaatcatta aagaaattac caaaactcat aatagagata
     8101 aatgctggaa cagcccatgg taacaagaat atcacaccaa agagacgttt tcctctgata
     8161 aaggattgat tagcaataac agccgttgca attccaataa caatttgaag acttgtcgct
     8221 gctaatgtcc agatcagcgt ccatgacaaa acagatgtaa atgctgtttt gaaggtgctt
     8281 aattctaaga tattaagaaa attcttcaac cctacccaat ccaataaacg atacggagga
     8341 atatgcctaa aatcgtaatt ggtaaaagca atcagcaagg tcactaaaac tggaaaaata
     8401 atagcgaaag ccattgctaa ataagccgga atgatgagca agtaaggaaa tcctttttca
     8461 taaatcgctt caagggtctc tttagccgtc accggaacct tcttgccttc attcatttga
     8521 tgtgcaacta atttagcatc tctcatagca accaaatgaa aaataccaaa taagactaga
     8581 gtaattaatt gcatggcccc cttaattaac ataaataagg agtgatcttg cattggaaca
     8641 cttccaagac tgtaaaaacc tgttaaagca ggaattccta gaactacaat ttctagtagt
     8701 tctaggaaga aaacaactaa taagactccc cctttaacga tttgtttatt gtatagttgt
     8761 cccaaacctg ggataacaga tagccacatg gctttattag gcttctgttg tctttggtta
     8821 tctaccattt tttattcctc ttctcataaa tgatttaagg ttatactcct tatcatacta
     8881 acttcctttt aagctgacta atttattttt ttatagtaat ggtttgtgct tcatgacctg
     8941 tctttatact ataagtctca ggacttgtcc ataaaacatt cccaatacta tcttttttaa
     9001 caagtttgac tgctatttct ttattgattg gtagatgagt atcaaagaac cagtttgggt
     9061 acttggcaat ggtctgagtg ttattaaata gaggaccaac agcattctta gcgtcatttg
     9121 cccccatctc aaaaacatca cccatgagat atagttgttc ccctggtact gtcttaaaat
     9181 cattgatgag taaacgaaca ggaatttgtt tatcagtcaa tacttcaaag gcttggtaac
     9241 tattgctggt ttgcttatcg gcagttgtca ctgaaatgtt ataataattt gctggaaccg
     9301 tcggtacttt taaggtgata agggtatctg accaagaaag gatctcagca gagatttctc
     9361 caaaagtcac ttgaccttta gaattaccaa aaccttgacc tgaaatagta atcttatttc
     9421 cagcaatacc cactgaagca tcgacatctc caagttgtgg tgtcttgtcc tctccttcat
     9481 aagtccatac agcgacttgc cccgctccaa gttcaaagct actaatagtg ccatctgctc
     9541 caactattaa ttcttgaccg ccaagaagac cttctaattt gtccttgtag ctattttggg
     9601 gcaaggctgt tttagcatta gtaattgtat aaccattcgt ttgatctcta ttaatagcca
     9661 ctagtgcgac atgattacca aatttacgct caaataccaa aacatgatca ctaatccaac
     9721 gttgttcagt tgttccataa gctaaagctt gattttgttt tcttaaagga gctagcttac
     9781 taatcacttt ataggcttgt gactctttat taaaacttgg catatcacca cgattattag
     9841 gatctttatc acctgttgca tactgctctg taccataata aatattaggc acacctctag
     9901 atgttaaaag caaagcatag gcttgattaa cagcagtttg attattagcc acttttgttg
     9961 cgaagcgttc catatcatga ttatcaataa aggtcacctg gtctgtcact tcattagtca
    10021 cctgatctct attttcaaga accttgtaaa aatctcgcat ggtaaacgtt gaaaaacctg
    10081 tatacaactg tctaatagca ttggcaaatc taaaatctaa aagccccatc ccactattgt
    10141 tagcaaatgt cgtcatatca taatcatcgt cggtatgtcc cgagaaccat tccccgaaaa
    10201 caaagacatt atgtttttca tagatatgac tcaaccaatt tttttgccaa ccttgtgaca
    10261 tatgtttaac agcgtcaact cggataccat caacacccag gtctaaccat ttatcaatag
    10321 cttctttcat gtactggtca actttcggat taatgttatt taaatcagct agtccgtaca
    10381 ttgaatgata aatagaattt tcataagtac taaaatcggt ccaagattca tgattaaaaa
    10441 tcttgtcttt atcatctgaa aatttaccaa ctaatttacc gtttttatat aaagcgccat
    10501 cttctttgaa agttgtgcct tctttttcgg ctgtagacgt atgattagga gcaaaatcaa
    10561 taactacctt aatatggtgt tgatgagcta ctttgactag ttgttgaaag tctgcttcag
    10621 tgccaaaatg ctggtttgtt ttaaagaagt ccttagccca atacccatga tatgcagcac
    10681 ttccattaga aggatcaata ctgtcgatat tttcaacagg agaagaaatc caaatggcag
    10741 aaatccccat atctgtcagg taaccatcct taatcttggc gatgattcct tgccaatcac
    10801 ctccatggta ttttttaagg tcatttttat caaaaacatc agtcttacca ttattagagg
    10861 tattaccatc gttaaaacga tctgttacaa tctgataaat cgtatctgtt gaaaaatctg
    10921 ctttgttggt gacagaagca ttatcggcag cagaaacagt tagtggaaat gaaattaagc
    10981 caagtaaaac agcacttttc gttaataact tataagtttt gatatgtaat tctctcatcg
    11041 ctttttcctt ttcaaataac cttttagtta ctaaacacaa tatatccatc ctggagtaat
    11101 tgttttttgt ttttagttac cccattgctt gtcaaaatct gtcctccttc cttaatagaa
    11161 taagcactct caccattgtt aagaacaagt gaaatgcttt gctcacctaa gctgcgtttc
    11221 agaatgaggc agtcttcgtc ataatcaatg ctaatagctc cttcttgaag agctggctgt
    11281 tggcgaagag caataatcgc ttttactttg ctaaagaaat ccttgtcccg ctcagcatca
    11341 tcccaaggaa aaccgcgacg cgaatcaggg tcgtaacccc cttccattgc cacttctgtc
    11401 ccataataca agcaaggaat gcccataaag ataaagagca atgctaaagc agctaacaat
    11461 ttatctttgg aacccttaac ttgagtgaag aaacgatgcg tatcatgaga atccaaaaga
    11521 ttcaagttca tgcgattgac ttgccaagtg tttcgcatca aaatgttgct taatttctca
    11581 gccaattgtt ttgatgtcat ggtttcaaaa gcaaagtaat ccaaacaggc tttggtgaaa
    11641 gcataattca ttatcccatc atactgatcc cctgccaaat aaggataggc atcatgccag
    11701 ttctccccaa ttaaaatggc atctgctttt tccgctttaa ccgcctgacg gaaacgtcgc
    11761 caaaaatcat gagatacttc atcagaaaca tctaggcgcc aaccatcgat acaaaattct
    11821 ttaatccagt atcgcccaat ctcaatcaga tagtcttgca cgtctctatt tgaggtattc
    11881 catttgggca tatagttaca tccagcaaag gtttcatagt ttactaggtc catggatggg
    11941 tgttcatcat gagtcataaa ccaatcaaaa aacttagact ctttaccgta tctcaagaca
    12001 tcctgaaatt ccacggcatc actgctggca tggttaaaga cagcgtctag aataatcttg
    12061 attcccattt ggtgagctaa atcaatcaat tcttgcaaat catatttagt tccaaattgt
    12121 ggatcaatag cgtagtaatc actaatatca tatttgtgat tactaatgga ttgaaaaatt
    12181 ggcgttaaat agatgacagt aatccctaaa tctttgaggt aatctaattt ttctgtaatt
    12241 ccctttaaat ctccaccagc aaaactttta ggagtcggtt tttctaacca acccatgttg
    12301 acataactgt cacctttttc ccaatcccca cggttaaagc gatcaacaaa gatttgataa
    12361 aaaaccgcgt cttttgtcca aggaatgact tgatgaacat caacagcatt gatataaggc
    12421 atttggaaga aattataaaa cccatttgaa aaatcgtaac tagtagttaa cccatcttca
    12481 ctaaaataat aggtttgacc ttcttcttca attttaaaaa tgtaggccaa cctcacatcg
    12541 tctaatgtta aagtagtttc ataataagca tgcgtatggt ctgtataggc aactgacatt
    12601 tccaattctt cttggaattc atgataacca tatttaggcc cgtggaccaa attaacagta
    12661 atacctctat cttctttggc cacgcgtaat cggatgacaa gttccttttg agatatggca
    12721 aagcaatacc tcgaatcagg aatatggagc attccggcaa cattcatttc tacctccatt
    12781 aataaagtat tgacgattac ttctttttct ttaaaacgaa aaaatagcag agcgactcat
    12841 acttctatat ttttagtatc tgtttcatct tcatcaaact aacagaaata gaagctgggc
    12901 gtacttcttt atcccagcct cccttctttg ccttattcag cgtatttttg ttcaatagct
    12961 tccttaatag tttttgctgc ttcttttgca gctttagatg cttcttcctt accagaagcc
    13021 acgttaaaga acatatttgc acctggttcc caaacttcag ccatttctgg aatatttggc
    13081 attggttgtg cgctctcaaa ctgactaatc acagctttcg ttaactcatt accctcttta
    13141 gaagcatagt tacgtgctgt taagttagct ggaatttctt gtgttttatc atagaaacgt
    13201 ttttggtttt cagcattggt cacataatca aggaattttt gagcagtcgt ttttccttta
    13261 gaatagtttg aaataaccca agctttacca cctgcgaacg gttggtattt tttaccattt
    13321 gtcaaagtag gaatttccat taccccaaag ttaacaccag catctttaaa ggagctagct
    13381 gcccatggac catcaataat gacaccagct tttttagaaa taaattgttc agtgataaaa
    13441 tcaccagctt ttttagtgtc ttgcattcct tgaggccaag tgccatacca ctgtttcgcg
    13501 tagtttaacc cttcaacagt tccgtcgtta cctaggccta aatcacttgg tttggttcct
    13561 ttatcaccaa agatataacc gccataaccg gcaattaaac cataaccata atagaagtct
    13621 gtccatttcg ccaagaaacc tacagatttt cctggttcag aggcaaaagc aaacttagaa
    13681 tctttttgta agacttctaa ttcagtaaag cttttaggtg cttgaggcac taaatcctta
    13741 ttataataag ttaccaaagt ttcaattacg tctggtgcac cataagtttt accatcaatg
    13801 gttactaatt tcttaaccgt atcatcaaac tctttgctgt ttcctaaagt aacctcagag
    13861 atttgacctt cagttcctaa gccacctaca cggtcaaaag gtgctaaaaa gacatctggt
    13921 gaagctcctg ttggaccatc cgttgatagc ttatcaagag tatccatcat accttctttt
    13981 ttgactttaa cggtgacttt atgctctttt tcaaactcac ccttaatact cttaatgtaa
    14041 tcaacatatc cctcatcaac agagactaca atagtctttc cgtctgtttc cttaacgcct
    14101 ttaccaccac cacttacgca agcagttagg ggtagcactg aagctaagga taaagttcca
    14161 agcacagatg cttttttcca attccatgac ataacatgtt cctcccgaaa tggttttttc
    14221 ttaaacacaa atcaaatcat ctatctactc tcatcatatt agcaaataaa agataacata
    14281 aggcatttaa aattaataac agtaatagtt tcgaaactta aacgtttaag ttaagtttat
    14341 cgtaatcgct ttcatttgtc aataactttt ttacttttct tttaagagca tatcagtagt
    14401 ttttctgcta attgaaaaca aggcatttac tgtcacacaa gtctgtcctt tcttttttct
    14461 acaactttat ttcgtcagcg taaataaaac aaaagctcta taatctcttg aatgggtaaa
    14521 cccactgtag agattataga gctttttgaa tatcaaaaaa gtcccataaa atttataatg
    14581 aaaagcaacc aaactctcat tagaaagact cttatggaac atatcaatca taccttacaa
    14641 cacatcggaa ttaaagacaa aaatatcact ctaaacaatg ttttgaagga tgaatctcct
    14701 actgagctcg ttactactta ccagaaaggt agataggaaa tcctgatgaa gagtgtgtcc
    14761 aagggattat caattaggtc ttagattaca aaatagtaat tcaggtaggc tctactgttt
    14821 tcaacactag ttctgcaatt tgttttcgag cttgcttagc ttctcttatt ggaaaaataa
    14881 catacgcatg aatcattttt tctgcaataa tcagctgatt agcaattcgt tgtcttgtta
    14941 attgatcatg taaagacata atgtctgggt aaaaaatttc ttttgtccca acaaaggtcg
    15001 ttatttcagg caaatcctca caatcaccat acaaaggact gacataagga agttgcatat
    15061 taccttgacc acctgcccat agttcaccta tatcacgaag acgacgagca tctaggtaag
    15121 cttcttctgc ttcaaaatcc ttgtaggctt gatttttaaa actaacatct aaccaaggag
    15181 atataagaat taattgcttt ggctgtagtg ctgttttagt atctcgtata tacttagcaa
    15241 acgccaaagc catcccccca ccagaagaat caccaatgac actaacttga ctcggtccat
    15301 tagacaagca aaattgctgg taagcttcaa ctaatttagg atagacatct tgataagtag
    15361 catacggtac tttaggaaag ttaggaaaaa caaccttggc attagtcagc tgtataagat
    15421 gcttaaccgt cttaaaatga agtttttcgg gtcgtcgtaa atagacactg ccataaagat
    15481 aataaataac aggctgatga ctttgctttt gatcattaat aataaaaact tgtatcccgt
    15541 tcacatcgta ttcttgagtt gaaaaaccta ctatctcttc aggattatct agatgataag
    15601 ggatattacg attacgctgc gtctcaaccc ccttataatt atcaactttt ttagataact
    15661 tatactttct ataagcaaac attttctcaa ccaaccaact tgaaaaggag cgcttttgac
    15721 catattcaac tatcaaaata cctagtatca ctccaaaaaa aatcaccaca gcaataatga
    15781 ccacttatta tcctcgtttt ctaatattat tttactattg caagtctttc ctatttgccc
    15841 aaattttact ataaaaataa gggttcattt tatagttggg cactggctgc ttcgtttcta
    15901 gaaatggttg cactttctta tcaaaagcta accagccatt ccaaccgaga tggatggtat
    15961 cttgcataaa gtaggactca ccaccatctt ttgagaagtc agcaatgcga tgaaatcctt
    16021 gtgactttaa ctggaatttt attttacgaa ctgccgcttg atacttatct tgatttaagc
    16081 cggtataatc cgcccaagct ttattaacag gagttataac aaagagtaca tctgtttttc
    16141 gtttagcaaa ttctgataat aaaagctgaa aatcattgta ttctggtgac gccaggtaac
    16201 tataattaac ttggaaattc ttataaagat tgtatttagg tgctattcgt ttacgataaa
    16261 atgtattttt aatcccaaaa cggttgttgg ttgttgctaa ttggcctctc ttagttgcta
    16321 acgcattcaa ttgtttatac gaaaatactt taggtaatcc cttaacgcga ggcaaaattc
    16381 ttttttcata gttagtagat ttgcctaaaa aactaaaaag ggactcttct ctcaatgcta
    16441 cttgatgttg acatttcaaa atagctctgt ctaaccgact aagagactta cccttactta
    16501 cttttttcaa taagtttgat ttagacacac cagggttaag ctcaagcaaa cgctttgctg
    16561 caaactgtga ttctttatca gttctagctt ttagtaaaaa ttcaatcact tgagtgttag
    16621 acaagtacat ctgaaccgca ctaggattaa tcccttgagc agtaaaccat tgaggagata
    16681 ctacaaagat ggcttttttc ttttgcattt cattggtaat ttgttgcata ccataataat
    16741 gcgacaaaga tgctgatcct ctcttaccaa ttaaaaatgg tctatagctc cgcttgtagc
    16801 gctctgcaag caccgaaggg tgcatactat ccattcgact ccattcgcta gaaccaaaaa
    16861 aaggcacaaa acgacaagtc tcatctgata aagcttgtct tttaatcaaa ccatttttaa
    16921 aagaactatc tgtgatcgca acggcatttg ctttttcctg agctatggaa tgatcaagtt
    16981 gtgtaggaaa actaaaaata gtaatcacta ctaaaacaaa ggcaataaga agaggaccta
    17041 gaattaacca gagtctctta agcatgttgc aattcctcta caccttgaac aatcttagtt
    17101 actgtgttcc aatcatcacg accaaattct gaaataggta ctttaatatt gaacgttgac
    17161 tctaactcaa caatcaattc gactgtccca agactatcca acacacctgc atcaaacaag
    17221 tcttcgtcca tcatctctga aacatcttcc ataaacaaac ggtcaaacaa ttcaattaca
    17281 gtttcttcaa tagacatttt tatacttcct tttattaatg tagtggtctg ttaaaccaaa
    17341 ggtcatttaa aaaaccagag aataacagaa gtgaaaacat gacaacgtgg aaagttacca
    17401 cgatacataa gacatggaat gccctgcttt gaaagagggg tgataagcct tttttcttac
    17461 ggtgacggtt gatttctttt ttcttacgaa tccaagcatc attaataata agtcctatac
    17521 catggaaaag cccataagcg atataatacc aggttaagcc atgccaaaat cccatgacta
    17581 gcatattaac cagataggca acacctgaag tgacatttcg gtttttaaag gttttatgct
    17641 taatcaatag atgaaccaat cgcataaaca cataatctct aaaccagaag gacaaactca
    17701 tgtgccatct attccaaaaa tctttcaagc ttgcagataa gaaaggcatg ttaaagtttt
    17761 ctggtgttcg aatacccaat aggtaggaaa taccaatcgc aaacatcgaa taaccagcaa
    17821 agtcaaaaaa gagattcaag ccatacacgt acataaccag aatagtttcc ttattaaagt
    17881 agccaccaac catcaaagcc ttattttcaa ggagaggaag taagatgcca cccaaacaat
    17941 aggaaatgat atgcttgtat aggaaaccta acatgagata catgactgcc ttattgagca
    18001 ttgcaaaata atcatcacgt tcaggaagtt tatgataatc ttcctgaaaa cgtctgaagc
    18061 gatcaatggg gccacttgag aaagtgggga agaaaatcat gaagcgaata aaatccggta
    18121 aacgaacctc ttggagagtc ccgtcacgca tctcaataat cattccaata cttttaaagg
    18181 tcaaatatga aatccctaga aaactaaaga gtgtttgaga agaaggacct gttaggggag
    18241 caactttgac ccagataagg gggaataagg ccatcgcaat agctaagtaa aacactcctg
    18301 cactattagc tttctcacgg tatttctggt aagcaaaaac actgatgatt tgccacaaaa
    18361 gataaaagag aaaagcaact aattgttgat aatgatctcc tccaaacata aagagaataa
    18421 ataccaaact taccaaggtt tcataagtgc ttaagcgctt ttgtttaaaa atcccaatga
    18481 tgactggtaa gaaagccaaa ataagataga caaagtaaat ggggttaccg taaggttcca
    18541 tgtagggaat gtggctaaaa aacatcatca tcggttattg acctcattaa tcaaagtttt
    18601 aatatctatc ttaccatttg gagttaatgg tagactgtct cgataaagaa acttagaagg
    18661 catcatataa ctcatcatat gatccttaac agaggctttg atagctttgg ttaactccaa
    18721 ttccctatca aaacgctctt taaccccatc tttgacaacg atataggcca agagattttg
    18781 aaccttatgt tctttattgt accttggaac agccacagcc gatgctacca ttggtgattg
    18841 attcaactgt tgggaaacat cttctagttc aatacgataa ccagcatatt taatctgaaa
    18901 atccaaacgc ccaccataca gcaaaatatt atcttctgtc agtgagccaa tgtcccctgt
    18961 atgataagca ggctgtcctt taaaagtaaa gaaagcttct gcggttttct caggattgtt
    19021 gaggtaacct tttgacacag caggccctgt aacaataatt tcaccctgct cccctgaagc
    19081 taactcctta ccatcttcat caataatata ggtcggagaa tctggtttgg ggtagccaat
    19141 cggcaagcgg gtataattat ccaccatttc tctggtaatt tcaatagcgg ataaggctac
    19201 agtcgcttct gttggtccat aggcattgat gatcttggca ctcgggaaac gttcaaagag
    19261 ttttcgagcc gtagaaacgg ttaattcttc gccatcaaaa taaaaatgcg ttaaagctgg
    19321 catcttagcc tggcaaaaat catcgctaag catggccata tcagcaaatg aaggagtcga
    19381 tgtccaaatg ccaactggaa gttgcgcaat cgttgtaaat aactgtttaa agtctgccac
    19441 caattctttt ggaagtgcaa ataaggttcc acctaaagcc aaggtaggtg cccagtacat
    19501 gacagaaagg tcaaaggaat agggtggctg cgccagcatt tgtggctgtt taggtacatc
    19561 aaatgctgca tcttcgatca tccagttggt aaagctcaaa agattgtcat gtgaaatttg
    19621 cacccctttt ggttggcccg ttgttccaga ggtgaaaata atgtaataat tatcatctcc
    19681 tttaacagaa tgtgttcgct catagggcat ttctgctaat tttgctgact caatctctga
    19741 tagtgagaca agggaaatcc cttcaatagt aagagggaat tcctcaatag caatgattaa
    19801 actgggcttt gcaatctcaa taatcgctaa aatacgttct ggtgctgaat gcacatctac
    19861 tgggatatag gcatgaccag atttggttaa agctacaaaa gtagctaaca tatcataagt
    19921 ttgcgcccca aaaaccaaaa ctggagattt tgccagtaaa gctaagctat ctataaatgc
    19981 agcaatgcta tcagaatctc ttttgagttg tccgtaagtg cggtgttccc ctaaacaatc
    20041 ataaactggg aaatcagcct gtgtctgagc aaactgctca atactgtcaa tcatatcctt
    20101 aatcatcagt caatctccta aaattcatta taaataaagt tactttgacc atgtcctaga
    20161 taaccaaaga aatagaccag caacattaaa atcagataaa ataaaattgt cttgccaata
    20221 aaaacagcaa ttcctctcat tcttgatttg ttttttatca tactttacct tcttgtatta
    20281 gtgaaataat gtcctaaaaa attctactcg aaaaaaccta actt
//
LOCUS       DATXQK010000028        20023 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.028, whole genome shotgun sequence.
ACCESSION   DATXQK010000028 DATXQK010000000
VERSION     DATXQK010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20023
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.028"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            148..1116
                     /locus_tag="VC394_001128"
     CDS             148..1116
                     /locus_tag="VC394_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEP5994601.1"
                     /translation="MVTVSKEQHLDMFLKMERIREFDSRINKLVRRGFVQGMTHFSVG
                     EEAANVGAVAHLSYDDIIFSNHRGHGQSIAKDMDLNKMMAELAGKATGVSKGRGGSMH
                     LADFEKGNYGTNGIVGGGYALAVGAALTQQYKGTNNIAVAFSGDGATNEGSFHESVNM
                     AATWKLPVIFFIINNRYGISMSINNATNTPHLYTRAEAYGIPGFYCEDGNDVMAVYET
                     MGKAVEHVRGGNGPAIVEVESYRWFGHSTADAGKYRTKEEVDEWKEKDPMIKYRTYLT
                     SEGIATDDELDAIQAQVKKEVDDAYEFAQNSPDPELSVAFEDVWVD"
     gene            1175..2173
                     /locus_tag="VC394_001129"
     CDS             1175..2173
                     /locus_tag="VC394_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEP5994602.1"
                     /translation="MSETKLMALREAVNLAMTEEMRKDENIFLMGEDVGVYGGDFGTS
                     VGMIEEFGPKRVKDTPISEAAISGAAIGAAITGLRPIVDVTFMDFLTIMMDAIVNNGA
                     KNNYMFGGGLITPVTFRVASGSGIGSAAQHSQSLEAWLTHIPGIKVVAPGNANDAKGL
                     LKSAIRDNNIVLFMEPKALYGKKEEVNQDPDFYIPLGKGDIKREGTDLTIVSYGRMLE
                     RVLQAAEEVAADGINVEVVDPRTLIPLDKELIIESVKKTGKLMLVNDAYKTGGFIGEI
                     ATMITESEAFDYLDHPIVRLASEDVPVPYARVLEQAILPDVEKIKAAIVKMANKGN"
     gene            2358..3767
                     /locus_tag="VC394_001130"
     CDS             2358..3767
                     /locus_tag="VC394_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEP5994603.1"
                     /translation="MAFEIIMPKLGVDMQEGEIIEWKKQEGDTVNEGDILLEIMSDKT
                     NMELEAEDSGVLLKIVRPAGDTVPVTEVIGYIGAEGESVDTIASSEKTTEIPVPASAD
                     AGPAVAPKENVASPAPQVAATAIPQGNGGKVRATPAARKAAAEMGIDLGQVPGTGPKG
                     RVHKADVENFKGAQPKASPLARKIAADKGIDLATVSGTGFNGKVMKEDIMAILEAAKP
                     AEAKAPAAKEEKVVDLAEGVEHKPMSAMRKAISKGMTNSYLTAPTFTLNYDIDMTEMI
                     ALRKKLIDPIMAKTGLKVSFTDLIGMAVVKTLMKPEHEYMNASLINDANDIELHRFVN
                     LGIAVGLDDGLIVPVIHGADKMSLSDFVLASKDVIKKAQTGKLKAAEMSGSTFSITNL
                     GMFGTKTFNPIINQPNSAILGVGATIPTPTVVDGEIVARPIMAMCLTIDHRLVDGMNG
                     AKFMVDLKKLMENPFELLI"
     gene            4098..5861
                     /gene="lpdA"
                     /locus_tag="VC394_001131"
     CDS             4098..5861
                     /gene="lpdA"
                     /locus_tag="VC394_001131"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEP5994604.1"
                     /translation="MAVEIIMPKLGVDMQEGEIIEWKKQEGDTVNEGDILLEIMSDKT
                     NMELEAEDSGVLLKITRQAGETVPVTEVIGYIGAEGESVEVSSPAASDVNVARTTEDL
                     EAAGLEVPKAPAQAASAAPKAALADDEYDIIVVGGGPAGYYAAIRGAQLGGKIAIVEK
                     SEFGGTCLNVGCIPTKTYLKNAEILDGIKIAAGRGINLASTNYTIDMDKTVDFKNTVV
                     KTLTGGVQGLLKANKVTIFNGLGQVNPDKTVTIGSQTIKGRNVILATGSKVSRINIPG
                     IDSKLVLTSDDILDLREMPKSLAVMGGGVVGIELGLVWASYGVDVTVIEMADRIIPAM
                     DKEVSLELQKILSKKGMKIKTSVGVSEIVEANNQLTLKLNNGEEVVAEKALLSIGRVP
                     QMNGLENLNLEMDRNRIKVNDYQETSIPGIYAPGDVNGTKMLAHAAYRMGEVAAENAI
                     HGNTTRKANLKYTPAAVYTHPEVAMVGLTEEQAREQYGDVLIGKNSFTGNGRAIASNE
                     AHGFVKVIADAKYHEILGVHIIGPAAAEMINEAATIMESELTVDELLLSIHGHPTFSE
                     VMYEAFADVLGEAIHNPPKRK"
     gene            complement(6534..8951)
                     /gene="hylA"
                     /locus_tag="VC394_001132"
     CDS             complement(6534..8951)
                     /gene="hylA"
                     /locus_tag="VC394_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronidase"
                     /protein_id="HEP5994605.1"
                     /translation="MNTYFCTHHKQLLLYSNLFLSFAMMGQGTAIYADTLTSNSEPNN
                     TYFQTQMLTTTDSEKKVVQPQQKDYYTELLDQWNSIIAGNDAYDKTNPDMVTFHNKAE
                     KDAQNIIKSYQGPDHENRTYLWEHAKDYSASANITKTYRNIEKIAKQITNPESCYYQD
                     SKAIAIVKDGMAFMYEHAYNLDRENHQTTGKENKENWWVYEIGTPRAINNTLSLMYLY
                     FTQEEILKYTAPIEKFVPDPTRFRVRAANFSPFEANSGNLIDMGRVKLISGILRKDDL
                     EISDTIKAIEKVFTLVDEGNGFYQDGSLIDHVVTNAQSPLYKKGIAYTGAYGNVLIDG
                     LSQLIPIIQKTKSSIEADKMATIYHWINHSFFPIIVRGEMMDMTRGRSISRFNAQSHV
                     AGIEALRAILRIADMSEEPHRLALKTRIKTLVTQGNAFYNVYDNLKTYHDIKLMKELL
                     SDTSVPVQKLDSYVASFNSMDKLALYNNKHDFAFGLSMFSNRTQNYEAMNNENLHGWF
                     TSDGMFYLYNNDLGHYSENYWATVNPYRLPGTTETEQKPLEGTPENIKTNYQQVGMTS
                     LSDDAFVASKKLNNTSALAAMTFTNWNKSLTLNKGWFILGNKIIFVGSNIKNQSSHKA
                     YTTIEQRKENQKHPYHAYVNNQPVDLNNQLVDFTNTKSIFLESDDSAQNIGYYFFKPT
                     TLSISKALQTGKWQNIKADDKSPEAIKEVSNTFITIMQNHTQDGDRYAYMMLPNMTRQ
                     EFETYISKLDIDLLENNDKLAAVYDHDSQQMHVIHYEKKATMFSNHNLSHQGFYSFPH
                     PVKQNQQ"
     gene            9184..10173
                     /locus_tag="VC394_001133"
     CDS             9184..10173
                     /locus_tag="VC394_001133"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007893175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEP5994606.1"
                     /translation="MKYIVNKSHNPAFNIALEAYAFRELVEEDELFILWINEPAIIIG
                     KHQNTIQEINKEYIDEHGIHVVRRLSGGGAVYHDLNNLNYTIISNKTAEGAFDFKTFS
                     QPVIATLADLGVTANFTGRNDIEIDGKKICGNAQAYYKGRMMHHGCLLFDVDMTVLGD
                     ALKVSKDKIESKGVKSVRARVTNILNELPEKITVEEFSDKILTKMKETYPDMTEYVLS
                     EDELAKIEQSAKEQFGSWDWTYGKAPEYTIERNVRYPAGKISTFANVENSIIKNLKIY
                     GDFFGIKDVQDIENLLIGCKYEYRDVFECLKTIDTTQYFSRMTVEEVAKAIVS"
     gene            complement(10286..11077)
                     /locus_tag="VC394_001134"
     CDS             complement(10286..11077)
                     /locus_tag="VC394_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEP5994607.1"
                     /translation="MTYTSLKSPENTDYLYDLNIAHLYGNLMNTYGDNGNVLMLKYVA
                     EKLGARVTVDIVSINDTFEQDDYDIVFFGGGQDYEQSIVAKDLPSKKAALADYIANNK
                     VVLAICGGFQLLGQYYVQANGVKIDGLGIMGHYTLNQHQNRFIGDIKIHNDEFNETYY
                     GFENHQGRTFLSGDEKPLGRVVYGNGNNKEDQTEGVHYKNVYGSYFHGPILSRNVNLA
                     YRLVTTSLKKKYGSAISLPSYDDILKQEITEEYADLKSKASFNKV"
     gene            complement(11077..12420)
                     /locus_tag="VC394_001135"
     CDS             complement(11077..12420)
                     /locus_tag="VC394_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEP5994608.1"
                     /translation="MKMKTLLGIIAGKAAQSILTKLGRGSTYPGRLALACNKDILKDL
                     SKDYDIVVVTGTNGKTLTTALTVGILKEAFGEIITNPSGANMITGITSTFLAAKKGKS
                     GRQIAVLEIDEASLPRITTYLKPSLFVYTNIFRDQMDRYGEIYTTYQMIVDGARNAPK
                     ATILANGDSPIFSSKDIVNPVQYYGFDTAKHAPQLAHYNTEGILCPKCEHILQYRLNT
                     YANLGDFICLNCGFKRPQLDYRLTELTAITHQSSEFVIDGQNYKINVGGLYNIYNALA
                     AVSVAEFFGVSPEKIKAGFNKSKAVFGRQETFTIGDKSCTLILIKNPVGASQALEMIQ
                     LADYPFSLSVLLNANYADGIDTSWIWDANFELITQMPITEINAGGVRHSEIARRLRVT
                     GFDDTKIKQAEKLEQIMEAIEKQESKHAYILATYTAMLEFRSLLADRHVVEKEMN"
     gene            12527..13378
                     /locus_tag="VC394_001136"
     CDS             12527..13378
                     /locus_tag="VC394_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEP5994609.1"
                     /translation="MNNLSSIDIKFLLSLFADPWLLAVHLLDILIVAYLIYRFIKALT
                     GTKIMSLVQGVIFFLVLRFIAEWIGFTTITYLMNQVITYGVIAGVVIFTPEIRAGLEK
                     FGRSTQVFLQKQYVSSESALVDALIKSVAYMGPRKIGALIAIEQTQTLQEYIATGIPL
                     NADISSQLLINIFIPNTPLHDGAVIVGQNKIVAACAYLPLSESKAISKEFGTRHRAAI
                     GLSENSDALTIIVSEETGAISVTRKGQFLHDLSTDEFETVLRTYLMSDSNVSLPWYKK
                     ILGGKSK"
     gene            13375..14331
                     /locus_tag="VC394_001137"
     CDS             13375..14331
                     /locus_tag="VC394_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEP5994610.1"
                     /translation="MKRFLNSRPWLGMVSVFFAILLFLTAASSNHNNSSSQIYSPIET
                     YTHSLKDVPIDMKYDSDKYFISGYSYGAEVYLTSTNRIKLDSEVNNDTRNFKIVADLT
                     HSHPGTVNVNLRVENLPSGVTATVSPDKISVTIGKKESKVFPVRGSVDAKQIANGYEI
                     SKIETGVNKVEVTSDESTIALIDHVVAKLPDDQVLDRNYSSRVTLQAVSADGTILASA
                     IDPAKTNLSVAVKKITKSVPIRVEAVGMMDDSLSDIQYKLSKQTAVISGSREVLEDID
                     EIIAEVNISDVTKNTSKTVSLSSSQVSIEPSVVTVQLTTTKK"
     gene            14385..15740
                     /locus_tag="VC394_001138"
     CDS             14385..15740
                     /locus_tag="VC394_001138"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEP5994611.1"
                     /translation="MGKYFGTDGVRGEANVELTPELAFKLGRFGGYVLSQHETERPKV
                     FVARDTRISGEMLESALIAGLLSVGIEVYKLGVLATPGVSYLVRTEKASAGVMISASH
                     NPALDNGIKFFGNDGFKLADDQELEIEALLDAPEDTLPRPSAEGLGTLVDYPEGLRKY
                     EKFLVTTGTDLSGMTVALDTANGAASVSARDVFLDLNAEIAVIGEKPNGLNINDGVGS
                     THPEQLQELVKETGADLGLAFDGDSDRLIAVDETGEIVDGDRIMFIIGKYLSEKGLLA
                     HNTIVTTVMSNLGFHKALDKQGINKAITAVGDRYVVEEMRSSGYNLGGEQSGHVIIMD
                     YNTTGDGQLTAIQLAKVMKETGKSLSELAAEVTIYPQKLVNIRVENSMKDRAMEVPAI
                     ANIIAKMEDEMAGNGRILVRPSGTEPLLRVMAEAPTDAEVDYYVDTIADVVRTEIGCD
                     N"
     gene            15864..16505
                     /locus_tag="VC394_001139"
     CDS             15864..16505
                     /locus_tag="VC394_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994612.1"
                     /translation="MTKQQIEKLADLLPPFAATLYLDMIPEFQEGYASYVYQTPKQDI
                     QKRRIKQLTKNFERLLDTPSFYVMPVTRKTAEQLLADKGLAYHLLSTVAANQVFAAAV
                     NEDQTLFQVLRNGARVAFVILQEKGEFLEMTLGILETVTENEVPAAIVEAVQTYLLAD
                     MQENLPSLSIMVSQESSKLFYQEQGFVLLAKGCKPGNENDSDVCQLVKYFSRP"
     gene            16568..17698
                     /locus_tag="VC394_001140"
     CDS             16568..17698
                     /locus_tag="VC394_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEP5994613.1"
                     /translation="MSKKPTSAYVHIPFCTQICYYCDFSKVFIQNQPVDAYLKALIQE
                     FDSYGIRDLKTLYIGGGTPTAITAKQLEYLLNHLERNLNLDDLEEFTIEANPGDLTPE
                     KIAVLQRSAVNRISLGVQTFNNKQLKQIGRSHNEEQIYSTIANLKTAGFHNISIDLIY
                     ALPGQTLDQVKENVAKALALDIPHLSLYSLILEHHTVFMNKMRRGKLNLPTEDLEAEM
                     FEYIISEMEANGFEHYEISNFTKPGFESRHNLMYWDNVEYFGCGAGASGYLNGIRYQN
                     RVPIQHYLKAVEAGNARLNEEVLRKEEMMEEELFLGLRKKTGVSIQRFQEKFGVSFEE
                     RYGNIVRELQNQGLLVKDDAFVRMTKKGLFLGDSVAERFILD"
     gene            17708..18460
                     /locus_tag="VC394_001141"
     CDS             17708..18460
                     /locus_tag="VC394_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEP5994614.1"
                     /translation="MGLSYQEELTLPFELCDVKSDIKLPLLLDYCLMVSGRQSAQLGR
                     SNNNLLVDYKLVWIVTDYEITIHRLPHFQETITIETKALSYNKFFCYRQFYIYDQEGG
                     LLVDILAYFALLNPDTRKVATIPEDLVAPFKTDFVKKPHRVPKMPLLEQSIDRDYYVR
                     YFDIDMNGHVNNSKYLDWMYDVLGCEFLKTHQPLKMTLKYVKEVSPGGQITSSYHLDQ
                     LTSYHQITSDGQLNAQAMIEWRAIKQTESETD"
     gene            18460..19224
                     /locus_tag="VC394_001142"
     CDS             18460..19224
                     /locus_tag="VC394_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEP5994615.1"
                     /translation="MPYKGYLIDLDGTIYQGKNRIPAGERFIKRLQERGIPYLLVTNN
                     TTRTPEMVQSMLANQFHVETGIETIYTATMATVDYMNDMNRGKTAYVIGETGLKSAIA
                     AAGYVEELENPAYVVVGLDSQVTYEMLAIATLAIQKGALFIGTNPDLNIPTERGLMPG
                     AGALNALLEAATRVKPVFIGKPNAIIMNKSLEVLGIQRSEAVMVGDNYLTDIMAGIQN
                     DIATILVTTGFTRPEEVPTLPIQPDHVLSSLDEWRL"
     gene            19224..19856
                     /locus_tag="VC394_001143"
     CDS             19224..19856
                     /locus_tag="VC394_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEP5994616.1"
                     /translation="MVENTKLLCSWVWLLALAILITIYSTWLWYPLEVDHLKLEQVVF
                     MSKDAILHNYNGLLNYLTNPFVTRLEFANFHSSADGLKHFADVKWLFHLTQVVFLGLL
                     YPTLKTFTQRLKTKRFWLLQKPLILAALFPLMIGLMASFIGFEHFFTLFHQVLFVGDS
                     SWLFDPLKDPVIWILPEVFFLHCFLFFMIVYEIILWSLVGLARWQRLNVE"
BASE COUNT         6173 a         3563 c         4177 g         6110 t
ORIGIN      
        1 ttttttagta cagttttgtt tgtaatttca caaactattg catttctaaa aaaccaaata
       61 aggtaaggta gaaagcgatt taattatttg caaattgtga aaaaaagttt tatcataaaa
      121 aggtaatatg ataatatgga ggccgaaatg gtaacagttt ctaaagaaca acatttggat
      181 atgttcctta aaatggaacg tattcgtgaa tttgattcac gtattaataa actcgtccgt
      241 cgtggatttg tacaaggcat gactcacttc tcagttggtg aagaagcagc caatgttgga
      301 gcggtggctc acttgtctta cgatgacatt attttttcaa atcaccgtgg acatggacaa
      361 tcaatcgcta aagacatgga ccttaataaa atgatggctg agcttgctgg taaagcgact
      421 ggtgtttcta aagggcgtgg tggctcaatg cacttggctg attttgaaaa aggaaactat
      481 ggtaccaatg gtatcgttgg tggtggttac gctcttgcag ttggtgctgc cctcacccaa
      541 caatacaaag gaactaacaa tatagctgtt gctttctcag gtgatggcgc tactaacgaa
      601 ggatcattcc acgagtctgt taatatggct gcaacatgga aacttcctgt tattttcttc
      661 atcatcaaca accgttatgg aatttcaatg agcatcaata atgcaacaaa tacaccacat
      721 ttatacacac gtgcagaagc ttacggaatt cctggtttct actgtgaaga tggcaatgac
      781 gtgatggctg tctatgaaac aatgggcaaa gccgttgaac atgttcgtgg tggaaatggt
      841 ccagctattg ttgaggtaga atcataccgt tggtttggtc actcgactgc agatgctggt
      901 aaataccgta ctaaagaaga agtggatgag tggaaagaaa aagatccaat gatcaaatat
      961 cgtacttatc tgacaagcga aggcattgcc actgatgacg aattggatgc tattcaagca
     1021 caagtcaaaa aagaagttga cgatgcttat gaatttgcac aaaacagtcc agatccagaa
     1081 ctttcagtag ccttcgaaga cgtttgggtt gactaagatc accttgtcag taagaagcaa
     1141 tcgtatttga ataataaatg gagaaataaa aatgatgtca gaaacaaaat taatggcttt
     1201 gcgtgaagca gtcaaccttg ctatgactga ggaaatgcgc aaggacgaaa acattttcct
     1261 tatgggagaa gacgtagggg tttacggagg agacttcggg acttctgttg gaatgattga
     1321 agaatttggt cctaaacgtg ttaaagatac cccaatttca gaagctgcta tctctggtgc
     1381 ggctattggt gcagctatta caggactacg tccaattgtc gacgttacct ttatggattt
     1441 ccttaccatc atgatggacg ctatcgtgaa taatggtgct aaaaataatt acatgttcgg
     1501 tggaggcctt attacccctg taaccttccg tgtggcatca ggttctggta ttggttccgc
     1561 tgcgcaacac tcacaatctc ttgaagcatg gttgactcac attccaggaa tcaaagttgt
     1621 tgctcctggt aatgccaacg atgctaaagg cttattaaaa tcagctatcc gtgataataa
     1681 cattgttctt ttcatggaac caaaagcgct ttatggtaaa aaagaagaag ttaaccaaga
     1741 tcctgacttc tatatcccac ttggcaaagg tgacatcaaa cgtgaaggaa ctgatttgac
     1801 aattgtgtca tatggtcgta tgttggagcg tgtccttcaa gcggctgaag aagttgcagc
     1861 ggatggtatt aacgttgaag ttgttgaccc acgtaccctc atcccacttg ataaagagtt
     1921 gattattgaa tctgttaaga aaacaggtaa attgatgctt gtcaacgatg cctacaagac
     1981 cggtggattc attggggaaa ttgcaaccat gattactgaa agtgaagcct ttgattacct
     2041 tgatcaccca atcgttcgtc ttgcaagtga agatgttcca gtaccttatg cacgtgtact
     2101 tgaacaagcg atcttaccag acgttgagaa aatcaaagct gccattgtga aaatggcaaa
     2161 caaaggcaac tagaagataa ggcaagtggt ctgataaagg ctatgtttat cagtaacgtc
     2221 tcagctaaaa actattttga aagtttttag aagaactgag ttgtcttatc ccgtgtcgcg
     2281 gcacctcacc tttcgtatgt tttcgtagca aggtggggat gttgatggcg cttatcaaat
     2341 cgtgaaagga tgtagaaatg gcatttgaaa ttattatgcc aaagctgggc gttgacatgc
     2401 aagaaggcga gatcatcgag tggaaaaaac aagaaggtga taccgtcaat gaaggtgata
     2461 tccttcttga aatcatgtca gacaaaacca atatggaact tgaggcagaa gactcaggtg
     2521 tgctattgaa aattgtccgt ccagctggtg atactgtacc tgtaactgaa gttatcggtt
     2581 acattggtgc agagggtgaa tcagtagata ctattgcttc aagtgaaaaa acaacagaaa
     2641 ttccagttcc tgcttcagca gacgctggac cagcagttgc tccaaaagaa aatgtggcaa
     2701 gtccagctcc tcaagtggca gcgacagcta ttccgcaagg aaatggcgga aaagttcgtg
     2761 ctactccagc tgcacgtaag gcagcagcag aaatgggcat tgaccttggc caagttccag
     2821 gaacaggacc aaaaggacgt gtccataaag cagacgttga aaactttaaa ggtgctcaac
     2881 cgaaagcttc accacttgct cgtaaaattg cagcagacaa aggtattgat ttggcaaccg
     2941 tatcaggtac tggctttaac ggtaaggtca tgaaagaaga tatcatggct attcttgaag
     3001 cggctaaacc agctgaagca aaagccccag cagctaaaga agaaaaagtt gttgatcttg
     3061 cagaaggtgt ggaacacaag ccaatgtcag ctatgcgtaa ggcaatctct aaaggtatga
     3121 caaactctta ccttactgct cctaccttta cgcttaacta cgatattgac atgacagaga
     3181 tgattgccct tcgtaagaag ttaattgacc caatcatggc taaaacaggc cttaaagtca
     3241 gcttcacaga cttgattggt atggcagtgg ttaaaacctt gatgaaacct gagcatgagt
     3301 acatgaatgc ttccctcatc aatgatgcta atgatattga attacaccgc tttgtgaacc
     3361 ttggtattgc cgtaggtctt gatgatggtc ttatcgttcc tgttatccat ggggctgaca
     3421 agatgagctt gtcagatttt gtgcttgctt caaaagatgt gattaaaaaa gcgcaaactg
     3481 gtaagttgaa agcagctgaa atgtctggat caaccttctc tatcacaaac ttgggaatgt
     3541 ttggtacgaa gacatttaac ccaattatta accaaccaaa ctcagcaatt cttggtgttg
     3601 gagcaactat cccaactcca acggttgtgg atggtgagat tgttgcccgt ccaattatgg
     3661 caatgtgcct aaccatcgac caccgtttag ttgatggtat gaacggtgca aaattcatgg
     3721 ttgatctcaa gaaattgatg gaaaatccat ttgaattgtt aatctgatca atcagtttta
     3781 aacaagaaaa gcattcaata ataaaaggga ttttgctaag tttagacttg gtgggtgccc
     3841 ttttctcaaa aatatagaaa ggatgtagaa cgaggagatc tcatgcaaat gagtgataag
     3901 caattgaatc cgagcgaaaa gctcggataa aaatggtaag agttggctaa tcttagcgtc
     3961 gtagacgtct gaactttcct caatttttgt ttcggaaagt cctagtcgtc ctgacgtggg
     4021 tgcgtcaacg aaatcaaaga tttctgactt accgcaaaaa ttcaatcgct ttctccttgc
     4081 tcttggtatc ttaaattatg gctgttgaaa ttattatgcc aaaactcggt gttgacatgc
     4141 aagaaggtga aatcatcgag tggaaaaaac aagaaggtga tactgtcaat gaaggcgata
     4201 ttcttcttga aatcatgtca gataaaacca atatggaact tgaagcagaa gactcaggtg
     4261 ttcttcttaa aattacacgt caagcaggtg aaacagtacc tgtaacagaa gttatcggat
     4321 atatcggtgc tgaaggtgaa tctgttgagg tttctagccc agctgcttca gatgtgaatg
     4381 ttgctcgtac aacagaagat ttagaagctg ctggacttga agtgccaaaa gcaccagctc
     4441 aagctgcttc agctgcacca aaagctgcac ttgctgatga tgagtatgac atcatcgttg
     4501 ttggtggtgg ccctgctggt tattatgcag ctattcgtgg tgctcaactt ggtggtaaaa
     4561 ttgccatcgt tgagaaatct gaatttggtg gaacttgctt gaacgtaggg tgtatcccaa
     4621 ccaaaactta ccttaaaaat gctgaaatcc ttgatggtat taagattgca gcaggacgtg
     4681 ggattaacct tgcctcaact aactatacca ttgacatgga caaaacagtt gactttaaaa
     4741 acaccgttgt taaaacctta acaggcggcg ttcaaggtct tctaaaagct aataaagtca
     4801 ctatctttaa tgggcttggt caagttaacc ctgacaagac tgtaaccatt gggtcacaaa
     4861 ccattaaggg tcgcaacgtt atccttgcta caggttctaa agtatcacgt atcaatatcc
     4921 caggaattga ctctaaactt gtcttaacgt ctgacgatat ccttgacctc cgtgaaatgc
     4981 caaaatcact agcagttatg ggcggtggtg ttgttggtat cgagcttgga cttgtttggg
     5041 catcttacgg tgtggatgtt accgttattg aaatggctga ccgcattatc ccagctatgg
     5101 ataaggaagt ctctcttgaa cttcaaaaaa tcctttctaa gaaaggcatg aagatcaaaa
     5161 catctgttgg tgtctctgaa attgttgaag caaataacca attgaccttg aaacttaaca
     5221 atggtgaaga agttgtcgct gaaaaagctc ttctttctat cggtcgtgta ccacaaatga
     5281 acggtctgga aaatcttaac cttgaaatgg atcgtaaccg tatcaaagtt aatgactacc
     5341 aagagacatc aattccaggt atctatgcgc caggtgacgt taacggaacg aaaatgcttg
     5401 ctcacgctgc ttaccgtatg ggtgaagtgg ctgcagaaaa tgcgatacat ggcaacacaa
     5461 ctcgtaaagc taaccttaaa tacactccag cagccgttta cacacaccct gaagtggcaa
     5521 tggttggttt aactgaagaa caagcgcgtg aacaatatgg tgatgtgctt attggtaaaa
     5581 acagctttac tggtaatgga cgtgcgattg cttcaaatga agcccatggt tttgttaaag
     5641 ttattgctga tgccaaatac cacgaaatct taggtgttca tattattggt ccagctgcag
     5701 ctgagatgat taacgaagca gctactatta tggaatctga attaaccgtt gatgaattgt
     5761 tattatcaat tcatggacac ccaaccttct ctgaagtgat gtacgaagcc tttgcagatg
     5821 tgcttggcga agctatccat aacccaccaa aacgtaagta aaacgtcttt gtcttattaa
     5881 ctaatgaaaa atatcatcac ataacagatc acaaaaaaga ctagcacaac tatgaactgc
     5941 accccaaacg ttagatataa aaccctaacg tttggggtgt ttttgtatga cattaagcta
     6001 tgaagataag ctagacattt agcgctaaaa aaacgagaga actccgtttg aaagaagcat
     6061 ctctgaaaag aagaagacaa aaaaccatta taaagaatgg acaaagacga gctctgatta
     6121 gattttcttt taaggacggc taaagtgact cgttctacct actattatca tcgattattg
     6181 caccaataac cgtatcaaaa taaaacgaaa aggacttagt cctctacaat acagaactaa
     6241 atcctttact tcaaatagag tagccaactt ttggggctca gtgcagtttc cagttttttg
     6301 tctttaataa cgtttcattg ttaaccctta ctttccaata acagctaaat ccttaagtct
     6361 ttcttacgaa agggttttgt gatatggtta aaagaagcaa ctaaagctcc acctaagaat
     6421 tctaaagagg aggactgata cccagtatgc ggtaagcgtc gttgtctttt gtggggaatt
     6481 ttatgagagt taagagcatt gtttttagct gcaattcctt gatgagcaag cttttattgt
     6541 tgattttgct tgacaggatg aggaaaacta taaaagcctt gatgagaaag attatgattt
     6601 gaaaacatcg ttgctttttt ttcatagtga atgacgtgca tctgttgact atcatgatcg
     6661 tagacagcgg ccagtttgtc attgttttct aacaagtcga tatcaagctt gctaatatag
     6721 gtttcaaatt cttgacgagt catatttgga agcatcatat aggcataacg atcgccatct
     6781 tgagtatggt tttgcatgat agtgataaag gtatttgaaa cttctttgat ggcttctggt
     6841 gatttgtcat cagcttttat gttttgccat ttccctgttt gaagtgcctt acttatgcta
     6901 agtgttgttg gcttgaagaa gtagtaacca atattttgag cagaatcatc actttcaagg
     6961 aaaatacttt tagtgtttgt aaaatcaact agctgattat tcaagtcaac gggttgattg
     7021 ttaacataag catggtaagg gtgcttttga ttttcttttc gttgttcaat agttgtatac
     7081 gccttgtgag atgattggtt tttgatattg ctaccaacaa agattatttt gtttcctaag
     7141 ataaaccacc ctttattgag agtgaggctt ttattccaat tagtgaaggt catagcagct
     7201 agagcacttg tgttattaag ttttttactt gcaacaaaag cgtcatcaga gagactagtc
     7261 atgccaactt gttgatagtt cgttttaata ttctcaggag ttccctctag tggtttttgc
     7321 tcagtttctg tggttccagg taagcggtag ggatttacag ttgcccaata gttttcactg
     7381 tagtgtccta aatcgttatt gtataggtaa aacattccat cagaagtaaa ccagccatga
     7441 agattttcat tattcatagc ttcataattt tgagttcgat tcgaaaacat tgataggcca
     7501 aaagcaaaat cgtgtttatt attatatagt gccaatttat ccatactatt gaaactagct
     7561 acgtaactat caagtttttg gactggaaca gaagtatcac ttagcagttc tttcataagt
     7621 ttgatatcgt gataggtttt caaattatca tagacattgt aaaaagcatt cccttgtgtg
     7681 acgagtgttt ttatacgtgt tttaagtgcc aaacggtgag gctcttcaga catgtcagca
     7741 atacgtaaaa tagcacgaag tgcttcaatg ccagcaacat gagattgagc attaaaacga
     7801 ctgatagaac gccctcgagt catatccatc atttctccac gaacgatgat agggaaaaaa
     7861 gaatggttaa tccaatgata gatagtagcc attttatccg cttctataga agactttgtt
     7921 ttttgaataa taggaattaa ttgcgataag ccatctataa gaacattacc gtaagctcca
     7981 gtgtaagcaa tgcctttttt ataaagtgga ctttgagcgt tagtaaccac gtgatcaatt
     8041 aaagaaccgt cttggtaaaa accatttcct tcgtcaacta gcgtgaaaac tttctcaatt
     8101 gctttgattg tatcactaat ttcgagatca tctttacgaa gaataccgga aatgagttta
     8161 acacgcccca tatcaattaa atttccgcta ttggcttcaa aaggtgaaaa attggcagcg
     8221 cgaaccctaa aacgagtagg gtcaggcaca aatttttcga ttggagctgt gtatttaagg
     8281 atttcttctt gagtaaaata aagatacatc aaggataagg tattattaat agcacgagga
     8341 gttccaattt cataaaccca ccaattttct ttgttttctt ttccagttgt ttgatgattt
     8401 tcacgatcta gattataagc gtgttcatac atgaaggcca taccgtcttt tacaatagca
     8461 atagctttac tatcttgata atagcatgac tcaggattag tgatctgttt tgctattttt
     8521 tcaatattgc ggtaagtttt cgtgatatta gcagaagcgg aataatcctt tgcatgttcc
     8581 caaaggtaag ttctattttc gtggtcaggc ccttgatagc ttttaataat gttttgagca
     8641 tccttttcag ctttattatg aaaagtgacc atgtcaggat tggttttatc ataagcatcg
     8701 ttgcctgcga taatactgtt ccattggtct aacaattcag tatagtagtc tttttgttgt
     8761 ggctgtacta cctttttttc gctatctgta gtagtgagca tttgcgtttg aaagtaagta
     8821 ttattaggtt ctgaatttga agtcagtgta tcggcataaa tggcagttcc ttggcccatc
     8881 atagcaaagc taaggaatag gtttgaataa agtagtaatt gtttatggtg tgtgcaaaaa
     8941 taagtattca ctgataaatt cctcctatat aaaaatgaga taataaaagt aaattggttt
     9001 actaagtaaa aatataccac gttaaagtat ttttataaac ccttacataa ataatggctg
     9061 agtttttgtg cactaataag tgtaggtact gaagatgtca tacagaatag gacacttgtc
     9121 ttttatattt gttttcatta tactagaaag gtagattaca tttttgaaag gattttttct
     9181 cctatgaaat acatcgttaa caaaagtcac aatcctgcct ttaacatcgc tttggaagcc
     9241 tatgctttta gagaactagt ggaagaagac gaattgttta tcctttggat caatgagcca
     9301 gctattatta ttggcaaaca tcaaaacacc attcaagaaa tcaataaaga atacattgat
     9361 gaacatggta ttcatgttgt gcgacgttta tctggtggtg gtgcggttta tcatgatttg
     9421 aataacttga actacactat catttcaaat aaaacagcgg aaggcgcatt tgatttcaaa
     9481 accttctcac aacctgtgat tgcgacttta gctgatttgg gtgtaacagc caactttaca
     9541 ggccgtaatg atattgaaat tgatggcaaa aaaatctgtg gtaatgctca agcttactac
     9601 aaaggtcgca tgatgcacca tggttgcctt ttgtttgatg tggatatgac cgttcttggc
     9661 gatgctctta aggtaagtaa ggataagatt gagtctaaag gagtgaagtc tgttcgtgct
     9721 cgcgtgacca atattttaaa tgaattacca gaaaaaatta ccgttgagga attttcagat
     9781 aaaatcttga ccaagatgaa agaaacgtat ccagacatga cagaatatgt cttgtcagag
     9841 gatgagttgg ctaagattga acaatctgcc aaagagcaat ttggtagttg ggattggacc
     9901 tacggcaaag cgccagaata tacgattgaa cgcaatgttc gttaccctgc aggtaaaatt
     9961 tcaacctttg caaatgttga aaattctatc attaaaaacc ttaaaatcta tggtgatttc
    10021 tttgggatca aagatgtgca agacattgag aacttattaa ttggttgtaa atatgagtac
    10081 cgtgatgttt ttgaatgttt aaaaacgatt gatacgaccc agtacttctc acgcatgacc
    10141 gttgaagaag ttgctaaagc tattgtttct tagtggtttt aatcacttaa gaagctctgt
    10201 cagaagaaaa aactataaaa ctcctaagcg aactatctat cgtgtcctgc ttaggagttt
    10261 ttgtttgatg acatgaaaga agaacttaaa ctttattaaa ggaagctttg cttttaaggt
    10321 cggcatattc ttcagtgatt tcttgtttca gtatatcatc atagctaggt agtgaaatag
    10381 ctgatccata ctttttttta agagatgttg ttactaagcg gtaagcaaga ttaacattgc
    10441 gtgataagat aggaccgtgg aaataactac catagacgtt tttatagtga acaccttcag
    10501 tttggtcttc tttattatta ccgttgccat aaaccactcg tccaagtggc ttttcgtcac
    10561 cagataagaa ggtacgtcct tgatgatttt caaagccata ataggtttcg ttaaattcgt
    10621 cattatgaat tttaatatct ccaataaagc gattttggtg ctgattaagg gtgtagtgtc
    10681 ccataattcc aagtccatca attttaaccc cattagcttg gacatagtat tgccctagta
    10741 attggaagcc accacagatg gcaagcacca ctttattatt agcaatgtaa tctgctaagg
    10801 cagctttttt gctaggcaaa tccttggcaa caatgctttg ttcataatct tgaccaccac
    10861 caaagaaaac aatatcgtaa tcgtcttgtt caaaggtatc attgatagat acaatatcaa
    10921 cagtaaccct agctccaagt ttttcagcga catacttgag catcaagaca ttcccattgt
    10981 caccgtaggt attcatcagg tttccgtaga gatgagcaat gtttaggtca tagaggtagt
    11041 cagtgttttc tggagatttt agtgaagtgt aagtcattag ttcatctcct tttcaacaac
    11101 atggcggtcg gctagcaagc tacggaattc taacattgca gtataggttg ctaaaatata
    11161 agcatgtttg ctttcttgtt tttcaatagc ttccatgatt tgctcgagct tttcagcttg
    11221 ttttatctta gtgtcgtcaa atcctgtgac tctaagtctt ctggcaattt cagagtgacg
    11281 gacgccgcca gcattaattt ctgtaatggg catttgggta atgagttcaa aattagcatc
    11341 ccaaatccag ctggtatcaa tgccgtcagc gtaattagca tttaaaagca cagataagct
    11401 aaatgggtaa tcagctagct gaatcatttc aagcgcttga ctggctccaa caggattctt
    11461 gataaggata agggtgcatg atttatcacc aatagtaaaa gtttcttgtc ttccaaaaac
    11521 cgccttactt ttgttaaagc ccgccttgat tttctcggga gaaacaccga aaaattcagc
    11581 aacagaaacc gcagcaagcg cattgtaaat attataaaga ccaccaacat ttatcttgta
    11641 gttttgtcca tcaataacaa attcagaact ttggtgagta atagcggtga gttcagttaa
    11701 tcgataatcc aactgtggtc tcttgaagcc acaatttaaa caaataaagt ctcctagatt
    11761 cgcataagta ttgagacgat actgaagaat atgttcgcat tttggacata aaatcccttc
    11821 tgtgttgtaa tgagctagct gcggagcatg cttagcagta tcaaaaccat aatactgaac
    11881 tggattaact atgtccttag aagagaaaat aggactatct ccattggcta gaatagttgc
    11941 ttttggagca tttcgagcac catctacaat catctgataa gtagtataaa tctctccgta
    12001 gcggtccatc tgatccctaa aaatattagt ataaacaaaa agactaggtt ttaagtaagt
    12061 ggtaattcta ggaagactag cttcgtcaat ttctaaaaca gcaatttgcc ttccagattt
    12121 ccctttttta gcagctaaaa aagtagaagt aattccagta atcatattgg caccacttgg
    12181 atttgtgatt atctcgccaa atgcctcttt caaaataccg acagtaagag cagttgttaa
    12241 cgttttacca ttagtaccag tgacgaccac aatatcatag tccttagaca aatcctttaa
    12301 aatatcttta ttacaagcta gagccaatcg accaggataa gtagatcctc gccctagttt
    12361 tgttaaaatg gattgagcag ctttgccagc aataatgccg agtaatgtct tcattttcat
    12421 gcttatattt tatcataagt ttattgattt tagataggga aaaatctttg atcatgatat
    12481 gatataatat agctaataac ttttttgata tagaaatgag agttgtatga ataatttatc
    12541 tagtatcgat attaaatttt tattaagctt atttgcggat ccttggctgc tggcagtgca
    12601 tcttttagat attctaattg ttgcctattt gatttatcgt tttattaaag cattgactgg
    12661 aactaaaatc atgtctttag ttcaaggggt cattttcttt ttggttcttc gttttattgc
    12721 agagtggatt ggctttacga caatcacata cttaatgaac caagtcatta cttatggtgt
    12781 aattgcagga gtagtcattt ttacccctga aattcgggca ggactggaga aatttggtcg
    12841 ttctacgcaa gtttttttac aaaaacagta cgtcagcagt gaatctgctt tggtcgatgc
    12901 tttgattaaa tctgtggcct atatgggtcc acgcaagata ggtgcattga ttgctattga
    12961 acagactcaa accttacaag aatatattgc gacaggaatt ccgctgaatg ccgacatttc
    13021 aagtcaacta ctcatcaata tttttattcc gaatacgccg cttcatgatg gagctgttat
    13081 cgttggtcag aataagattg tggcagcctg tgcttacttg cctttgtcag aatcaaaggc
    13141 aatttccaaa gaatttggga cccgtcatag agcggctatt ggattatctg aaaactctga
    13201 tgcactaact atcattgttt cagaagaaac aggagctatt tcagtaactc gcaaaggcca
    13261 atttttacat gatttaagca cagatgaatt tgaaacggta cttaggacct acctgatgag
    13321 tgattcaaat gttagtttac catggtataa gaaaattcta ggagggaaat ctaaatgaaa
    13381 cgatttttaa atagtcgtcc ttggttagga atggtatcag tcttttttgc gattctcctt
    13441 tttttaactg cagcatcgag caatcataat aattctagct cgcaaattta tagcccgatt
    13501 gaaacctata cgcatagcct taaagatgta ccaattgata tgaagtatga tagtgacaaa
    13561 tattttatca gtgggtattc ttatggtgcg gaagtttatc taacctccac taatcgtatc
    13621 aaattagatt ctgaagtgaa caatgataca cgtaatttta agattgtggc ggatctaaca
    13681 catagtcatc caggtacagt aaatgtcaac ctaagagtag aaaaccttcc ttctggggtg
    13741 acagctaccg tatctccaga taaaatatca gtcacaattg gcaaaaaaga atctaaggta
    13801 tttcctgtta gaggaagcgt tgatgctaaa caaattgcaa atggttatga aattagcaaa
    13861 attgaaacgg gggttaataa ggttgaagtg accagtgatg aatctaccat tgctttaatt
    13921 gatcacgttg tggcaaagtt acctgatgat caagtgttag ataggaatta tagtagtcga
    13981 gtgaccttac aagctgtctc agctgatgga actattttag caagtgcaat tgatcctgca
    14041 aaaacaaatt tatcagttgc tgtaaaaaaa ataacgaaat cagtcccgat tagagttgag
    14101 gcagtgggga tgatggatga tagcttatca gatattcaat acaaattgtc aaaacaaaca
    14161 gctgttatct caggtagtcg agaggtctta gaagatattg atgaaattat tgcagaagtt
    14221 aacatttctg atgttactaa aaatactagt aagaccgtga gtttgtcttc tagtcaggtg
    14281 tcaattgagc cgtcagtagt gaccgttcag ttgacaacca ccaaaaaata atggataata
    14341 actaggaaaa ttgaatggag gaagccatta ggcttatata aattatggga aaatattttg
    14401 gaacagatgg tgttcgtggc gaagcaaatg ttgagctgac acctgagtta gcttttaaac
    14461 tgggccgatt cggtggttat gttttaagtc aacatgagac cgaaagacct aaagtttttg
    14521 tggcgcgtga cacccgtatt tcaggagaaa tgttggagtc agccttgatt gctggtctac
    14581 tatctgttgg tattgaagtt tataaacttg gcgttttagc gacacctggt gtctcttatt
    14641 tagttagaac agaaaaagca agtgcaggtg tgatgatttc tgcaagtcat aaccctgcgc
    14701 ttgacaatgg catcaaattc tttgggaatg atgggtttaa gttagctgat gaccaagaat
    14761 tggaaattga agctctctta gatgctccgg aggacacgct tcctcgcccg agtgcagaag
    14821 gacttggaac cttggtagat tatccagaag gacttcgtaa atatgaaaag ttcttggtaa
    14881 caactggtac tgacttaagc ggtatgacag ttgccttaga cactgctaat ggagctgctt
    14941 cagtgtcagc acgtgatgtc tttttagatt taaatgcaga aattgctgtc attggtgaaa
    15001 aaccaaatgg tcttaatatt aatgacggtg ttggttcaac gcatccagaa caacttcaag
    15061 agttagttaa ggaaacagga gcagatcttg gtttagcctt tgatggcgat agcgaccgtc
    15121 tgattgccgt ggacgaaact ggcgagattg ttgacggtga tcgtattatg tttatcattg
    15181 gtaaatactt gtcagaaaaa gggcttcttg ctcacaatac cattgtcaca acggtgatgt
    15241 ctaacctcgg ttttcataaa gctttagata aacaaggtat taataaagca attacagcag
    15301 ttggagaccg ctatgtggtt gaagaaatgc gtagctcagg atacaatctt ggtggtgaac
    15361 aatcaggcca tgtcattatc atggactaca ataccactgg agatggtcag ttgacagcta
    15421 tccaattagc caaggtgatg aaggaaactg ggaaatcact ttcggaatta gctgctgaag
    15481 taaccattta ccctcaaaaa ctggtgaata ttcgtgtgga aaacagcatg aaagaccgtg
    15541 ctatggaagt tccagctatt gcgaacatca ttgcaaaaat ggaggatgaa atggctggta
    15601 atggtcgtat tctcgtgaga ccaagtggta cagaacctct tcttcgtgtc atggcagagg
    15661 cgccaacaga tgccgaagtt gattattatg tcgatactat tgctgatgtc gtccgtacag
    15721 aaatcggatg cgataactaa cgagttgagt acaaacaaga aaccttattt ctaaggcttc
    15781 tgtcaacaat tggaaagagg cttggggcgt ttagcccaag tctcttttta taaattgcta
    15841 aagtagaaga gaaaggatat cggatgacaa aacagcaaat cgaaaaacta gcggacttac
    15901 tgcctccttt tgcagccaca ctttacttag atatgattcc tgaatttcag gaaggttatg
    15961 cttcatatgt ttatcaaaca ccaaaacaag atattcaaaa gcgccgcatc aagcagttaa
    16021 ctaaaaattt tgaacgatta ctagataccc catcttttta tgtgatgcct gtgacaagaa
    16081 aaacagcaga gcaattgcta gcagataaag gcctagctta tcatttgtta tcgacagttg
    16141 cagctaatca agtgtttgcg gctgctgtta acgaggatca gacgcttttt caagtgcttc
    16201 gaaatggagc aagagtagcc tttgtgattc ttcaagaaaa gggggaattt ctagaaatga
    16261 ctttagggat tcttgaaaca gtgacagaaa acgaggttcc agcggctatt gtggaggctg
    16321 tccagacata tcttctggca gatatgcaag agaacctccc ttctttaagc attatggtca
    16381 gtcaagaatc aagcaagctt ttttaccaag aacaaggatt tgttttgcta gctaagggtt
    16441 gcaagccagg aaacgaaaat gattctgacg tttgtcagct cgtaaaatat ttttccaggc
    16501 catgaagaat caagggaaat ggcttattca tagaaacatt ttatcatcaa agtggtataa
    16561 taagaccatg tcaaaaaaac ctacttcagc ctatgtgcac attccttttt gcacacaaat
    16621 ttgttattat tgcgattttt caaaagtctt tatccaaaat caacctgttg atgcttattt
    16681 gaaagccttg attcaagaat ttgactctta tggcattcga gacctaaaaa ccctttatat
    16741 tggaggaggt acgcctacag ctatcactgc taaacagttg gagtatctcc taaatcacct
    16801 tgaacgaaac ctcaatttag atgatcttga agaattcaca attgaggcaa atcctggtga
    16861 tttgacgcca gaaaaaattg cggtgcttca gaggtcagct gtcaatcgga tttccttggg
    16921 ggtgcagact tttaataaca agcaattaaa gcagattggc cgtagccata atgaagaaca
    16981 aatttatagc accattgcca atttaaaaac tgcaggcttt cacaatattt ccattgattt
    17041 aatttatgcc ttgccaggtc aaaccttaga tcaagtcaag gaaaatgttg ctaaagctct
    17101 ggcacttgat attcctcacc ttagtcttta tagccttatt ttggaacatc acactgtttt
    17161 tatgaacaaa atgcgacgtg gtaaacttaa cttaccaacg gaagacttgg aagcggaaat
    17221 gtttgagtac attatttcag aaatggaagc taatggtttt gagcattatg aaatttccaa
    17281 ttttaccaaa ccaggttttg aaagtcgcca taacttgatg tattgggata atgtggagta
    17341 ttttggatgt ggtgcagggg cttcaggtta ccttaatggc attcgttatc agaatcgggt
    17401 gcccatccag cattatctta aagcggttga agcagggaat gctagactaa atgaagaagt
    17461 gctgcgcaaa gaggaaatga tggaagagga actctttttg ggcttacgta aaaagacagg
    17521 agtctccatc cagcggttcc aagaaaaatt tggtgtttct tttgaggagc gttatggaaa
    17581 tattgttagg gagcttcaaa accaaggact gcttgtcaaa gatgacgctt ttgtcagaat
    17641 gactaaaaag ggtcttttct taggtgattc tgttgctgag cggtttatat tagattagga
    17701 gagtattatg ggattaagtt atcaggagga gttgacactt ccttttgaat tatgtgatgt
    17761 caaatcagat ataaaattgc cccttttatt agactattgt ttgatggttt ctggtagaca
    17821 gtctgcgcaa ttaggacgaa gtaacaacaa ccttttagtc gattacaagc ttgtttggat
    17881 tgtaacggat tatgagatca ctattcatcg cttgccacat tttcaagaaa ccatcaccat
    17941 tgaaacaaaa gccctctcct acaataaatt tttttgttat cgccaatttt atatttatga
    18001 tcaagagggg ggtcttttag tggatatctt agcctatttt gctttgttaa acccagatac
    18061 gcgaaaagtg gcaactattc cagaagattt agtagcgcct tttaagactg attttgttaa
    18121 aaagccacac cgtgttccta aaatgcctct tttagaacaa tcaattgatc gtgattatta
    18181 tgtgcgttat tttgatattg atatgaatgg tcatgtcaac aacagtaaat atttagattg
    18241 gatgtatgat gtgttggggt gtgagttttt aaaaacgcat cagcctctta agatgacttt
    18301 gaaatatgtt aaagaagtct caccaggcgg tcaaattact tccagttacc atttggatca
    18361 attaacttct taccatcaaa tcacctcaga tgggcagctg aatgcccaag ccatgattga
    18421 atggcgagcg attaaacaaa cagaaagcga gacagactag tgccttataa gggttattta
    18481 attgatttag acggaacgat ttaccaaggg aaaaaccgta ttccagcagg ggagcggttt
    18541 atcaaacgcc tacaagaaag aggcattccc tatttattag tgactaataa taccacaaga
    18601 acacccgaaa tggtgcaaag catgctggct aatcagtttc atgttgagac aggcattgag
    18661 accatttata cggctaccat ggcaaccgtc gattacatga atgacatgaa ccgtggcaag
    18721 acagcttacg ttattggaga aacagggctc aaatcagcta ttgcagcagc aggttacgta
    18781 gaagaacttg agaatcccgc ttacgtggtg gttggtttag acagtcaggt gacctatgag
    18841 atgttggcta tagccacctt ggctattcaa aaaggtgccc tctttattgg gacaaatcct
    18901 gatttaaaca ttccaaccga acgtggtttg atgccaggag caggtgcatt gaatgcctta
    18961 ctagaagctg ccacacgtgt aaaacctgtt tttattggca agcccaacgc tatcattatg
    19021 aataaatcac tagaggtatt gggcattcag cgctctgagg ctgtcatggt tggcgataat
    19081 tacttgactg atatcatggc aggtattcaa aatgatattg caacgatcct cgtgacaaca
    19141 ggctttacca gacctgaaga ggtaccaacc ttaccaattc agccagatca tgtcctatct
    19201 agccttgatg agtggaggtt gtgatggtag aaaacactaa attactatgt agctgggtat
    19261 ggttattagc gttagctatt ctcataacga tctattcaac gtggctgtgg tatcctcttg
    19321 aggtggacca tctaaagtta gaacaagttg tttttatgag caaggatgct atcttgcata
    19381 actataacgg actactaaat tacctcacca atccttttgt gacaaggttg gaatttgcta
    19441 attttcactc atcagcagac ggactcaagc attttgcaga tgtgaagtgg ctttttcacc
    19501 taactcaagt cgtttttctt ggcctccttt acccaaccct taagactttc acacaaaggt
    19561 taaaaaccaa acgtttctgg ctccttcaaa aacctttaat actagcagct ttgtttcctc
    19621 ttatgattgg tctaatggct agtttcattg ggtttgaaca tttttttact ctttttcatc
    19681 aggtgctttt tgtaggagat agcagctggc tctttgatcc cttgaaagat cctgtgattt
    19741 ggatccttcc agaagtcttt ttcttgcact gtttcctttt ctttatgata gtttacgaaa
    19801 tcatcctgtg gagcttagta ggtttagcaa gatggcagcg cctaaatgta gaatgataaa
    19861 acaagcgttt tgaaagcgct tgttttttag tttgcagtca gagtagaata gaagtatcaa
    19921 aaaaaagcac cgactcggtg ccactttttc aagttgataa cggactagcc ttattttaac
    19981 ttgctatgct gttttgaatg gttccaacaa gattatttta taa
//
LOCUS       DATXQK010000029        19796 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.029, whole genome shotgun sequence.
ACCESSION   DATXQK010000029 DATXQK010000000
VERSION     DATXQK010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19796
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.029"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            32..964
                     /locus_tag="VC394_001144"
     CDS             32..964
                     /locus_tag="VC394_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_101244918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEP5994617.1"
                     /translation="MGKRMSLILGAFLSVFLLVACSSTGTKTAKSDKLKVVATNSIIA
                     DMTKAIAGDKIDLHSIVPIGQDPHEYEPLPEDVEKTSNADVIFYNGINLEDGGQAWFT
                     KLVKNAQKTKNKDYFAVSDGIDVIYLEGASEKGKEDPHAWLNLENGIIYSKNIAKQLI
                     AKDPKNKETYEKNLKAYVAKLEKLDKEAKSKFDAIAENKKLIVTSEGCFKYFSKAYGV
                     PSAYIWEINTEEEGTPDQISSLIEKLKVIKPSALFVESSVDRRPMETVSKDSGIPIYS
                     EIFTDSIAKKGKPGDSYYAMMKWNLDKISEGLAK"
     gene            1028..1753
                     /locus_tag="VC394_001145"
     CDS             1028..1753
                     /locus_tag="VC394_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004218966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEP5994618.1"
                     /translation="MITTNNLCVTYDGNSNALEAINVTIEGPSIVGIIGPNGAGKSTF
                     MKAILNLIDYQGHVTVDGKDGRKLGHTVAYVEQRSMIDYNFPITVKECVALGTYSKLG
                     LFRRVGKKQFEQVDKVLKQVGLEDFGHRPIKSLSGGQFQRMLVARCLIQESDYIFLDE
                     PFVGIDSVSEKIIVDLLKELKMAGKTILIVHHDLSKVEHYFDKLMILNKHLVAYGNVC
                     EVFTVDTLSKAYGNHLILGKEMV"
     gene            1754..2608
                     /locus_tag="VC394_001146"
     CDS             1754..2608
                     /locus_tag="VC394_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEP5994619.1"
                     /translation="MSMKFFEGLMSYHFLQNALITAVVIGIVSGAVGCFIILRSMSLM
                     GDAISHAVLPGVALSFILGVNFFIGAIIFGLLASVIITYIKENSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDSDKWITIGVSIFVLVVISLFFKELLLT
                     SFDPILAKSMGVKVNAYHYLLMVLLTLVAVTAMQSVGTILIVALLITPAATAYLYANS
                     LKVMLVMSSLLGALASVLGLYLGYTFNVAAGSSIVLTSAMMFLISFFVSPKQGYLKRW
                     MQKKEKTP"
     gene            complement(2755..3561)
                     /locus_tag="VC394_001147"
     CDS             complement(2755..3561)
                     /locus_tag="VC394_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEP5994620.1"
                     /translation="MKKLLSLSLVAISLLSLSACESVDRAIKGDKYIDEKTAKEESEA
                     ASKAYEESIQKALKADASQFPQLTKEVGKEEAKVVMRTSQGDITLKLFPKYAPLAVEN
                     FLTHAKKGYYDNLTFHRVINDFMIQSGDPKGDGTGGESIWKGKDPKKDAGNGFVNEIS
                     PFLYHIRGALAMANAGANTNGSQFYINQNKKNQSKGLSSTNYPKPIISAYEHGGNPSL
                     DGGYTVFGQVIDGMDVVDKIAATSINQNDKPKQDITITSIDIVKDYRFKN"
     gene            3778..6183
                     /locus_tag="VC394_001148"
     CDS             3778..6183
                     /locus_tag="VC394_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEP5994621.1"
                     /translation="MVKRNQRRKSAPKKCLTKAEVEKQRAIKRMILSVLMALLLIFAM
                     LRLGVFGVTTYNMIRFLVGSLAYPFMFAWLIYLFCFKWLRQKDGMIAGVVIAFLGLLV
                     EWHAFLFAMPRMLDQDIFLGTARLITRDLLALRVTEFVGGGMLGALLYKPIAFLFSNI
                     GSYFIGFLFILLGLFLMTPWDIYDVSHFVKEAVDKLAVAYQENKEKRFIKREEHRLQA
                     EKEALEKQAQEEEKRLAELTVDPETGEIVEDSQSQVSYDLAEDMPKEPEILAYDSHLK
                     DDEASLFDQEDLAYAHEEIGAYDSLSALASSEDEMDMDEPVDVDFTPKTHLLYKLPTI
                     DLFAPDKPKNQSKEKNLVRKNIKVLEDTFQSFGIDVKVERAEIGPSVTKYEIKPAVGV
                     RVNRISNLADDLALALAAKDVRIEAPIPGKSLIGIEVPNSEIATVSFRELWEQSDANP
                     ENLLEVPLGKAVNGNARSFNLARMPHLLVAGSTGSGKSVAVNGIISSILMKARPDQVK
                     FLMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFSKVGVRNIA
                     GYNAKVEDYNRQSEQKQMPLPLIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGIH
                     MILATQRPSVDVISGLIKANVPSRIAFAVSSGTDSRTILDENGAEKLLGRGDMLFKPI
                     DENHPVRLQGSFISDDDVERIVNFIKDQAEADYDDAFDPGEVSDNDPGFSGNGGAAEG
                     DPLFEEAKALVLETQKASASMIQRRLSVGFNRATRLMDELEEAGVIGPAEGTKPRKVL
                     QTN"
     gene            complement(6253..6606)
                     /locus_tag="VC394_001149"
     CDS             complement(6253..6606)
                     /locus_tag="VC394_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEP5994622.1"
                     /translation="MMIYKLTALAFLIVTPLFAMIVTPLFKLNKKGFKFPDVALLLFA
                     IEIVLVSGKFFTHNLLPYYLIIMSLLAIVISLLLVIRTQSFSYHRFMKLFWRVGFLVT
                     VIFYLILVIFIFTLA"
     gene            6850..7275
                     /gene="rplK"
                     /locus_tag="VC394_001150"
     CDS             6850..7275
                     /gene="rplK"
                     /locus_tag="VC394_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEP5994623.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFDFITKTPPAAVLLKKAAGVEKGSGTPNTTKVATVTR
                     AQVQEIAETKMPDLNAANIEAAMRMIEGTARSMGFTVTD"
     gene            7381..8070
                     /gene="rplA"
                     /locus_tag="VC394_001151"
     CDS             7381..8070
                     /gene="rplA"
                     /locus_tag="VC394_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEP5994624.1"
                     /translation="MAKKSKQMRAALEKVDSTKAYSVEEAVALVKETNFAKFDASVEV
                     AYNLNIDVRKADQQIRGAMVLPNGTGKTQRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINGGWLDFDVVIATPDMMAIVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADKAGNVQALIGKVSFDADKLVENFKAFHDVMAKAKPATAKGTYMANVSITSTQ
                     GVGIKVDPNSL"
     gene            8392..9120
                     /locus_tag="VC394_001152"
     CDS             8392..9120
                     /locus_tag="VC394_001152"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEP5994625.1"
                     /translation="MEPKYQRILIKLSGEALAGEKGVGIDIPTVQAIAKEIAEVHVSG
                     VQIALVIGGGNLWRGEPAADAGMDRVQADYTGMLGTVMNALVMADSLQHYGVDTRVQT
                     AIPMQNVAEPYIRGRALRHLEKNRIVVFGAGIGSPYFSTDTTAALRAAEIEADAILMA
                     KNGVDGVYNADPKKDANAVKFDELTHGEVIKRGLKIMDATASTLSMDNDIDLVVFNMN
                     EAGNIQRVVFGEHIGTTVSNKVCD"
     gene            9149..9706
                     /gene="frr"
                     /locus_tag="VC394_001153"
     CDS             9149..9706
                     /gene="frr"
                     /locus_tag="VC394_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEP5994626.1"
                     /translation="MANAIIETAKERFAQSHQSLSREYASIRAGRANASLLDRIQVDY
                     YGAPTPLNQLASITVPEARVLLISPFDKSSIKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRKELAKEVKKVGENAKIAIRNIRRDAMDDAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAIKEIDRMTAEKEKELLSV"
     gene            9815..10672
                     /locus_tag="VC394_001154"
     CDS             9815..10672
                     /locus_tag="VC394_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEP5994627.1"
                     /translation="MNDLLATVITGLIKEENANDYFIQKEGFTFTLSKAEGERQIGDM
                     VTGFAYTDIKQKARLTTKEIRSTRTSYGWGEVTEVRRDLGVFVDTGIPNKEIVVSLDV
                     LPEMKELWPKKGDKLYIRLDVDKKDRIWGLPAEPEVFQKMASPVYNNMQNQHWPAIVY
                     RLKLTGTFVYLPENNMLGFIHPSERYAEPRLGQVLDVRVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMIVTYLEANGGFMTLNDKSSPEEIKATFGISKGQFKKALGGLMKAKRIKQ
                     DATGTELIG"
     gene            10745..11254
                     /gene="msrA"
                     /locus_tag="VC394_001155"
     CDS             10745..11254
                     /gene="msrA"
                     /locus_tag="VC394_001155"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEP5994628.1"
                     /translation="MERAIFAGGCFWCMVQPFEEQAGILSVRSGYTGGHLPNPSYEQV
                     CAKTTGHTEAVEIIFDPEEISYKELVELYWVQTDPTDAFGQFEDRGDNYRPVIYYTTE
                     RQKEIAEQSKANLQASGRFDQPIVTTIEPAEPFYLAEDYHQGFYKKNPRRYAQSSAIR
                     HQFLEENWS"
     gene            11251..11466
                     /locus_tag="VC394_001156"
     CDS             11251..11466
                     /locus_tag="VC394_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEP5994629.1"
                     /translation="MRKSFYSWLMTQRNPKSNAPVAILADLVFDDTTFPKHTNDFELI
                     SRYLEDQASFSFNLGQFDEIWEDYLAH"
     gene            11622..12803
                     /locus_tag="VC394_001157"
     CDS             11622..12803
                     /locus_tag="VC394_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEP5994630.1"
                     /translation="MIITKKSLFVTSVALSLAPLATAQAQEWTPRSVTQIKSELVLVD
                     NVFTYTVKYGDTLSTIAEAMGIDVHVLGDINHIANIDLIFPDTILTANYNQHGQATTL
                     TVQAPASSPASVSHVPSSEPLPQASATSQSTIPMAPSATPSDVPTTPLASAKPDSFVT
                     ASSELTSSTNDVSTELSSESQKQPEVPQEAVPTPKAAETTEVEPKTDISEDSTSANRP
                     VPNESASEEASSAAPAQAPAEKEETSQMLTAPAAQKAVADTTSVATSNGLSYAPNHAY
                     NPMNAGLQPQTAAFKEEVASAFGITSFSGYRPGDPGDHGKGLAIDFMVPVSSTLGDQV
                     AQYAIDHMAERGISYVIWKQRFYAPFASIYGPAYTWNPMPDRGSITENHYDHVHVSFN
                     A"
     gene            13118..14890
                     /locus_tag="VC394_001158"
     CDS             13118..14890
                     /locus_tag="VC394_001158"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEP5994631.1"
                     /translation="MYYTSGNYEAFATPRKPEGVDQKSAYIVGTGLAGLAAAVFLIRD
                     GHMAGERIHLFEELPLAGGSLDGIEKPHLGFVTRGGREMENHFECMWDMYRSIPSLEI
                     PGASYLDEFYWLDKDDPNSSNCRLIHKRGNRVDDDGQYTLGKQSKELVHLIMKTEESL
                     GDQTIEEFFSEDFFKSNFWIYWATMFAFEKWHSAVEMRRYAMRFIHHIDGLPDFTSLK
                     FNKYNQYDSMVKPIIAYLESHDVDIQFDTKVTDIQVEQTAGKKVAKTIHMTVSGEAKA
                     IELTPDDLVFVTNGSITESSTYGSHHEVAKPTKALGGSWNLWENLAAQSDDFGHPKVF
                     YQDLPAESWFVSATATIKHPAIEPYIERLTHRDLHDGKVNTGGIITITDSNWMMSFAI
                     HRQPHFKEQKDNETTVWIYGLYSNSEGNYVHKKIEECTGQEITEEWLYHLGVPVDKIK
                     DLASQDYINTVPVYMPYITSYFMPRVKGDRPKVIPDGSVNLAFIGNFAESPSRDTVFT
                     TEYSIRTAMEAVYSFLNVERGIPEVFNSAYDIRELLKAFYYLNDKKAIKDMDLPIPAL
                     IEKIGHKKIKDTFIEELLKDANLM"
     gene            15049..16101
                     /locus_tag="VC394_001159"
     CDS             15049..16101
                     /locus_tag="VC394_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEP5994632.1"
                     /translation="MQEYSIDITLTHPDDVLALFGSNERHLKLIEAHLGVIVHARTER
                     VQVIGDDEEAVELARLTIKALLVLVGRGMVVNTSDVVTALSMAESHQIDQFMALYEEE
                     IIKDNYGKAIRVKTLGQKTYVDSVKRHDVVFGVGPAGTGKTFLAVTLAVTALKRGQVK
                     RIILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYHILGKEQTTRLMERDVIEIA
                     PLAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFNSKMIVNGDTSQIDLPRNVKS
                     GLIDATQKLQGIKQIDFVYFSAKDVVRHPVVADIIKAYETSSEEMKDLLKPKASDVEA
                     DYQPGLTKYPVIGQEH"
     gene            16147..16722
                     /locus_tag="VC394_001160"
     CDS             16147..16722
                     /locus_tag="VC394_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEP5994633.1"
                     /translation="MDDLTKAIMADEANLSYTERGIFPLYDAPQTARLIIVGQAPGIV
                     AQETKLYWNDRSGIRLRDWLGVDNDTFYHSGLFGIIPMDFYYPGKGKSGDLPPREGFA
                     AKWHPPLRALMPEVELTILVGRYAQDFYLGNKAYKTLTETVRHFEDYLPDYFPLVHPS
                     PRNQLWLAKNPWFEQDLLPILQKRVEAILTK"
     gene            16881..17378
                     /gene="ybeY"
                     /locus_tag="VC394_001161"
     CDS             16881..17378
                     /gene="ybeY"
                     /locus_tag="VC394_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEP5994634.1"
                     /translation="MYIEMIDETGQVSQEIMEQTLDLLNFAAQKTGKEEKEMSVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPETPILFSQEDFAADPSLAEMMAEFDAYIGEL
                     FISIDKAREQSQEYGHSFEREMGFLAVHGFLHINGYDHYTLEEEKEMFTLQEEILTAY
                     GLTRQ"
     gene            17359..17766
                     /locus_tag="VC394_001162"
     CDS             17359..17766
                     /locus_tag="VC394_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEP5994635.1"
                     /translation="MALHDNNTTKRKWKNRTITSSLEFALTGVFTAFKEERNLRSHLL
                     SACLACVAGLFFSISAIEWLFLLLAIFLVITLEIVNSAIENVVDLASDYHFSMLAKNA
                     KDMAAGAVLMISGYAVLTGLIIFIPKIWNIFVH"
     gene            17886..18782
                     /gene="era"
                     /locus_tag="VC394_001163"
     CDS             17886..18782
                     /gene="era"
                     /locus_tag="VC394_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEP5994636.1"
                     /translation="MFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKIM
                     GIYTTETEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVETVLFMVPADEKRGKGDD
                     MIIERLKAAKIPVILVINKIDKVHPDQLLEQIDDFRSQMDFKEVVPISALEGNNVPTL
                     IKLLTDNLEEGFQYFPEDQITDHPERFLVSEMVREKVLHLTQQEVPHSVAVVVESMKR
                     DEETDKVHIRATIMVERDSQKGIIIGKQGAMLKKIGKMARRDIELMLGDKVYLETWVK
                     VKKNWRDKKLDLADFGYNEKEY"
     gene            18802..19278
                     /locus_tag="VC394_001164"
     CDS             18802..19278
                     /locus_tag="VC394_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEP5994637.1"
                     /translation="MPQDYISYIRSKVGHDKIILNFAGGILTNDDGKVLMQLRGDKKT
                     WAIPGGTMELGESSLETCKREFLEETGIEVEAVRLLNVYTHFEEVYPNGDAVQTIVFI
                     YELTAVSDMAIDNFHNEETLKLQFFSHEEIAELESVSAKHRLMLEEYFSDSFAMGH"
     gene            complement(19584..19763)
                     /locus_tag="VC394_001165"
     CDS             complement(19584..19763)
                     /locus_tag="VC394_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994638.1"
                     /translation="MRYPHAHIILIGNHINYEEIFKNHYRVFGVIDTTSHKSLKSIRD
                     QIQLYLDELYNSNNG"
BASE COUNT         6114 a         3496 c         4294 g         5892 t
ORIGIN      
        1 aaataaataa tatatcctaa ggagagaaat gatgggaaag agaatgagcc tcattttggg
       61 tgctttttta tctgtttttc ttttagtagc ttgttcgtca actggcacta aaactgctaa
      121 gagtgataaa ttaaaagtcg tggcaaccaa ttcaattatt gccgacatga caaaagctat
      181 tgctggtgat aaaatcgatc tgcacagcat tgtgccaatc ggtcaagacc ctcatgagta
      241 cgaaccatta ccagaagatg ttgaaaaaac aagtaatgct gatgtgattt tctataatgg
      301 tatcaatcta gaagatggtg ggcaagcttg gttcaccaaa ctagtgaaaa atgctcaaaa
      361 aacgaaaaac aaagattact ttgccgtgtc tgatggcatt gatgtgattt acttggaagg
      421 tgcaagcgaa aaaggaaaag aagatccaca tgcttggtta aatctcgaaa acggaatcat
      481 ttattcaaaa aacattgcca aacaattgat tgcaaaggat cctaaaaaca aagaaactta
      541 tgaaaagaac ctaaaagctt atgtggctaa attggaaaaa ctagacaaag aagccaaatc
      601 aaaatttgat gctattgcag aaaataaaaa attgattgtg actagtgaag gctgcttcaa
      661 gtacttttca aaagcttacg gtgtcccatc tgcttatatc tgggaaatta acaccgaaga
      721 agaaggaaca ccagatcaaa tttcatcatt gattgaaaaa ctaaaagtca tcaagccatc
      781 tgcgcttttt gtagagtcaa gtgtcgatag acgccctatg gaaaccgttt ctaaagatag
      841 tggtattcct atttattctg agatctttac agattcaatt gctaaaaaag gtaaacctgg
      901 cgatagttat tatgctatga tgaaatggaa ccttgacaaa atttctgaag gtctagcaaa
      961 ataagataac aataaagcac agcgatttta gtgttgtgct ttgttttatt tatgtaaagg
     1021 agacaaaatg attacaacta acaatctttg tgtgacctac gatggcaaca gtaatgcttt
     1081 agaagcgatt aatgtgacaa ttgaaggtcc ttctatcgtt ggtattattg gtcctaatgg
     1141 agctggaaaa tcaaccttta tgaaagctat tttaaatctt attgattatc aaggtcacgt
     1201 aacggttgat ggcaaagatg gtcgtaagct tgggcataca gtagcttacg tagaacaacg
     1261 aagcatgatt gactataatt ttcctattac tgttaaagaa tgtgtggcgc tagggactta
     1321 tagtaaatta ggcctttttc gccgagtagg aaaaaaacaa tttgagcagg ttgataaggt
     1381 tctcaaacaa gttggtttag aagactttgg ccatcgaccg ataaaatcct tatctggtgg
     1441 tcagtttcag cggatgttgg ttgcgcgttg cttgattcaa gaatcagact atattttctt
     1501 agatgaaccg tttgttggca ttgactcggt cagcgaaaaa atcattgtag atcttttgaa
     1561 agagctcaaa atggctggca aaacgatttt aattgtacat catgatttga gcaaagtaga
     1621 gcattatttt gacaaactca tgattttaaa taaacatttg gtggcttatg gcaacgtttg
     1681 tgaggtattt actgttgata ccttgtcaaa agcctatggc aatcatttga ttttaggaaa
     1741 ggagatggtg taaatgtcta tgaaattttt tgagggcttg atgtcatacc actttttaca
     1801 aaatgcactg ataacggctg tagtcattgg tatcgtctca ggtgctgtag ggtgttttat
     1861 tatccttagg tcaatgtctc ttatgggtga tgccatctca cacgctgttt taccaggggt
     1921 tgctttgtca tttattttag gagtcaattt ttttattgga gcgattattt ttgggttatt
     1981 agcttctgtt attattactt atatcaagga aaactctgtc attaaaggag atacggctat
     2041 tggtatcacc tttagctctt ttttggcact gggagttatc ttgatagggg tagccaatag
     2101 ttcgacggac ttatttcata ttttgtttgg gaatatttta gctgtgcaag atagcgataa
     2161 gtggattact attggtgttt cgatttttgt tttagtggtt attagtctct ttttcaaaga
     2221 actattatta acatcatttg atcctatctt agccaaatca atgggtgtga aggtcaatgc
     2281 gtatcattat ttgttgatgg tcctattaac tttagtggct gttacggcga tgcaaagcgt
     2341 gggtactatc ttgattgttg ccttactgat tacgccagca gcgacagctt atttatatgc
     2401 taatagctta aaagtaatgt tagtgatgtc atctttacta ggtgctttag catcggtttt
     2461 agggctttat ttgggctata cctttaatgt tgccgcaggg tcaagtatcg tactgacttc
     2521 tgcgatgatg tttttgatca gtttctttgt ttcacccaag caaggctacc tcaaaagatg
     2581 gatgcaaaaa aaagaaaaaa caccttaaac ttttcgcttt aaacacctta gtggtttgca
     2641 aatagtggca aataacatca aagggtgttt tttatatgtc atcaaaagac cataaaaaac
     2701 cttatcaaac caaggtattt agcctattat ctgataaggt tttttaagca tgatttagtt
     2761 tttaaagcga taatctttga caatgtcaat tgaggtaatc gtaatgtctt gttttggttt
     2821 atcattttgg ttgatagaag tcgcggcaat tttatcgaca acatccatac catcaatgac
     2881 ttgaccaaat acagtataac cgccatctaa gcttggattg ccgccatgct cataggcaga
     2941 gatgataggt tttgggtagt tggtacttga taatcccttg ctttgatttt tcttgttttg
     3001 gttgatataa aattggctac cgttagtatt agcgccagca tttgccatgg caagagcacc
     3061 gcgaatatga tataaaaatg gagagatttc gttgacaaag ccattgccag catctttttt
     3121 aggatccttg cccttccaaa tcgattcacc acctgtgcca tctcctttgg ggtcacctga
     3181 ttgaatcata aagtcgttga tcacacgatg gaaggtaagg ttatcatagt agcctttttt
     3241 agcatgggtg aggaagttct caacagctaa gggagcatat tttgggaata acttaagggt
     3301 gatgtcccct tgacttgtcc tcattacgac cttagcttct tctttgccga cttctttggt
     3361 tagttgtgga aattggctag catcagcttt gagagctttt tgaatgcttt cttcataggc
     3421 tttagaagct gcttcgcttt cttctttggc agttttttca tcaatgtatt tatcgccttt
     3481 tatagcgcgg tcaacagatt cacaagcact caaactaagt aaactaatgg ctactaaact
     3541 gagagataat aattttttca tgtctactcc taagaatgat tttctttatt ttatcataaa
     3601 atgcaacaga aaatagtagt aaagagttaa aaacgcctat ttttgtgttt tatgatctaa
     3661 ccttggttaa aaggctgcta cgactgttat tatagcacaa gatgctatag aaaaacttaa
     3721 aacaccttgg ttttttagag ctatttccta atagttaaaa aatggtataa tagtgttatg
     3781 gttaaaagaa atcaaagaag aaaaagtgcg cctaagaagt gtctgacaaa agcagaagtg
     3841 gaaaagcaac gtgccattaa gaggatgatt ttatctgttt taatggcttt gttgcttatt
     3901 tttgcgatgc ttcgcttggg cgtctttggc gtgacaactt ataatatgat tcgttttttg
     3961 gtgggtagtt tagcctatcc gtttatgttt gcctggttaa tttacttatt ttgttttaaa
     4021 tggttacggc aaaaagatgg aatgattgcg ggagttgtta ttgccttttt gggattgcta
     4081 gttgagtggc acgccttctt atttgcgatg cctaggatgc ttgatcaaga tatttttcta
     4141 ggcactgctc ggctaattac aagagattta ctagccctgc gcgtgactga atttgttggt
     4201 ggaggaatgc ttggtgcatt actttataaa cccatagcct ttttattttc gaatatcggc
     4261 tcttacttta ttggtttttt gtttatctta ttaggattat ttctaatgac gccttgggat
     4321 atctatgatg tgagtcactt tgtgaaagag gctgttgata aactagcagt ggcttatcag
     4381 gaaaataaag aaaagcggtt catcaaacga gaagagcacc gcttacaagc tgaaaaagag
     4441 gctttagaga aacaagcaca ggaggaagaa aaacgtctag cagaattgac ggttgatcct
     4501 gagacgggag agattgttga ggatagtcag tctcaagtca gctatgattt ggctgaggat
     4561 atgccaaaag agccagagat tcttgcttat gatagtcacc tcaaagacga tgaggcaagc
     4621 ttatttgacc aagaagactt ggcttacgct catgaggaaa ttggggctta tgactcttta
     4681 tctgctttag catcttctga agatgagatg gatatggatg aacctgttga tgttgatttc
     4741 acgccaaaaa cccatcttct ttataaactt ccaacgatag atttatttgc accagataag
     4801 cccaaaaacc agtccaaaga gaaaaactta gtccgcaaaa atatcaaggt gcttgaagat
     4861 accttccaaa gttttgggat tgatgtcaag gtagagcgag cagaaatcgg gccttccgtc
     4921 accaaatatg aaatcaagcc agctgtcggg gtccgagtta atcgtatttc aaatcttgct
     4981 gatgatttgg cacttgctct ggctgctaaa gatgttcgta tagaagcacc aattcctgga
     5041 aaatccttaa taggtattga agttcctaac tcagaaattg caacggtttc tttccgcgaa
     5101 ctttgggaac aatctgatgc caatcctgaa aaccttttag aagtaccact aggtaaagct
     5161 gttaatggta acgctcgcag ttttaactta gctagaatgc cgcatctttt ggtagctggt
     5221 tcaactggtt caggtaaatc ggttgctgtt aatggcatta tttcaagtat tttgatgaag
     5281 gcacgtccag accaagtgaa atttttgatg gtcgacccta agatggttga gttgtctgtt
     5341 tataatgata ttccccattt gctgattcca gtagtgacca atcctcgtaa agccagcaag
     5401 gcgttgcaaa aagtggttga tgagatggaa aatcgctatg aactctttag taaagtcggg
     5461 gttcgaaata ttgctggtta caatgcaaaa gttgaggatt acaataggca atctgagcaa
     5521 aaacaaatgc ctttaccatt gattgtggtt attgtggatg agttagctga tttgatgatg
     5581 gtggctagta aagaagtgga agacgctatt atccgtctgg ggcaaaaggc gcgtgcggca
     5641 ggtattcaca tgattcttgc aacccaaaga ccatctgtgg atgttatttc tggactgatt
     5701 aaggccaatg tgccatcccg tattgcattt gccgtatctt caggaacaga tagtcgaacg
     5761 attctggatg aaaatggcgc tgaaaagctc ttaggacgtg gagatatgct ctttaagccg
     5821 attgatgaaa atcaccctgt tcgtctgcaa gggtctttca tctcagatga tgatgtggag
     5881 cgtattgtca actttatcaa agaccaagct gaagcggatt atgatgatgc ctttgatcct
     5941 ggtgaagtta gcgacaatga tcctggtttt tctggaaatg gcggagctgc tgaaggtgat
     6001 cctctttttg aagaagcaaa agccttggtc ttagaaacac aaaaagctag tgcctcaatg
     6061 attcaaaggc gtttgtcggt tggatttaat cgtgccacgc gcttgatgga tgaattggaa
     6121 gaagcaggtg ttatcggtcc agcagaagga accaaacctc gtaaggtatt gcagacaaac
     6181 taaaagaacc tgacgtctca tgatgagatg tcaggttctt tttttgtgat gtcaagccag
     6241 ttgatgagat gcctatgcta atgtaaaaat aaagataact agaatcaggt agaaaataac
     6301 ggtgactaaa aagccaactc gccaaaatag cttcataaag cgatggtaag aaaacgactg
     6361 agttcggatg actagcagta gtgaaatgac gatggctagc agcgacatga taataaggta
     6421 ataaggcagt aaattatggg tgaaaaattt tcctgagaca agcacaattt ctatcgcaaa
     6481 aagtaaaagt gcgacatctg gaaatttaaa tcccttttta ttaagtttaa ataaaggggt
     6541 tacaatcatg gcaaaaagag gcgttactat caagaaagct aaggcagtta atttatagat
     6601 catcataggt ctattttata ctcaaatccc ttacttgtaa actgatactt acctgttgtg
     6661 atagaaaatg atctttgaga gggaaagcgc ctacttcaat ttaaacatta caaaaggcat
     6721 cagctatttt aacaaaaaga cttgatttat tttggcaata agctataatg gtagggtatg
     6781 caatattgca tatctgtggg aggtaaaaat ctcaattacc gccaaaacca caacaggagg
     6841 atttttaaaa tggctaaaaa agtcgaaaaa cttgtaaaac ttcaaatccc tgctggtaaa
     6901 gctacaccag ctccaccagt tggaccagct cttggtcaag caggtatcaa catcatgggc
     6961 ttcactaaag aatttaacgc tcgtacagct gatcaagctg gtatgatcat cccagttgtt
     7021 atctcagttt atgaagacaa atcatttgat ttcatcacta aaacaccacc agctgctgtt
     7081 cttttgaaaa aagctgcagg tgttgaaaaa ggatcaggta cacctaacac tactaaggtt
     7141 gcgacagtta ctcgtgcaca agtacaagaa attgctgaaa ctaagatgcc agatttgaac
     7201 gctgcaaaca ttgaagctgc aatgcgtatg atcgaaggta ctgctcgttc tatgggattc
     7261 actgttactg actagtcagt caagaacccc tattacccgc acgacttcat cataagagat
     7321 gagtgacgtg ggagatttta aatcaaatcg atatgaccac attacaagga gaattttaaa
     7381 atggctaaaa aaagcaaaca aatgcgtgct gcacttgaaa aagtagatag cacaaaagcg
     7441 tacagtgtag aagaagctgt agcattagta aaagaaacga acttcgcaaa atttgatgcg
     7501 tctgttgaag ttgcttacaa cttgaacatt gacgttcgta aagcagacca acaaatccgt
     7561 ggcgcaatgg tattgccaaa cggaactggt aaaacacaac gtgttcttgt ttttgcacgt
     7621 ggtgcaaaag ctgaagaagc aaaagcagct ggtgcagact tcgtaggtga agacgacctt
     7681 gttgcaaaaa tcaatggtgg atggcttgac tttgacgttg ttatcgcaac gccagacatg
     7741 atggctatcg taggtcgtct tggacgtgtc cttggacctc gtaacttgat gccaaaccct
     7801 aaaactggta cagtaacgat ggatgttgct aaagccgttg aagagtctaa aggtggtaaa
     7861 atcacttacc gtgctgacaa agcaggtaac gtacaagctc ttattggtaa agtttcattt
     7921 gatgctgaca aattggttga aaacttcaaa gccttccacg atgtaatggc taaagctaaa
     7981 cctgcaacag ctaaaggaac ttacatggca aacgtctcaa tcacatcaac acaaggtgtt
     8041 ggtatcaagg ttgatcctaa ctcactttaa gaaaaaaatt agggcatcgc ctaatcgtag
     8101 caaaaagaca aatttctgaa gacaaatctt cactagaggt ttgtcttttt tatggtttaa
     8161 aatggaatgc aaacaaaaaa gtgtaggcga taataccatg aaaggagttg attctcttaa
     8221 tgagatgtgg tgttaacaca tcatgagctc acaaaaaatg ataaaggaag tatcggtggt
     8281 gttaaaaaag ggattttaat tgacatttat tagcctctaa aatgtccttt ccctattcat
     8341 aattgagggt ttgtggtaaa atagtaagga caaactaagg agaacatagt agtggaacct
     8401 aaatatcaac gtatattaat taagttatct ggtgaagcat tagcaggtga aaaaggagtt
     8461 ggcattgaca ttccaactgt tcaggctatt gcaaaagaaa ttgccgaagt tcatgtgtcg
     8521 ggagtacaga tcgctcttgt tattggtgga ggcaatctct ggcgtggaga gcctgcagca
     8581 gacgctggaa tggaccgtgt tcaggctgat tacactggca tgcttggaac ggtgatgaat
     8641 gccctagtca tggctgatag tcttcaacat tatggtgttg atacccgtgt gcaaacagct
     8701 attcctatgc aaaatgtagc agaaccttat attcgcggac gtgccttgcg ccatctagaa
     8761 aaaaatcgga ttgttgtttt tggagctggt attggctcgc cttatttttc aacagacacc
     8821 accgcggcac tgcgtgctgc tgagattgaa gcagatgcta ttttaatggc aaaaaatggt
     8881 gtggatgggg tctataatgc tgatcctaaa aaagatgcta atgcggttaa atttgatgaa
     8941 ttaacacatg gtgaagtgat caaacgtggt ctcaaaatca tggatgcaac ggcatcgacc
     9001 ttatcgatgg acaacgatat tgatctggtg gttttcaata tgaatgaagc tggcaatatt
     9061 caacgtgttg tctttggaga acatattgga actactgtat caaataaagt ttgtgactaa
     9121 aagttagaaa agaaagagag taattatcat ggcaaatgca attattgaaa ctgcaaaaga
     9181 acgttttgca caatcccatc agtctttatc acgtgaatat gccagcattc gtgcgggtcg
     9241 tgccaacgct agtcttttag accgtatcca agttgattat tacggggcac cgacgccatt
     9301 aaatcaattg gcttcaatca ctgtgccaga agcgcgtgtc ttattgatct cgccttttga
     9361 taaatcttct atcaaagata tcgagcgcgc tcttaacgca tcagatttag gcattacacc
     9421 tgctaacgat ggctctgtca ttcgtttggt tattccagct ttgaccgaag aaacacgtaa
     9481 agaattagcc aaagaagtga aaaaagttgg tgaaaatgct aaaattgcaa tccgtaatat
     9541 ccgccgtgat gctatggatg atgctaaaaa gcaagaaaaa gcaaaagaaa tcactgaaga
     9601 tgagttaaaa acacttgaga aagatatcca aaaagtaacc gatgatgcta tcaaagaaat
     9661 tgaccgcatg acggctgaaa aagaaaaaga attgctctca gtgtaacagg atttctacga
     9721 aaaatagacg actatagaca caaaaagata ggtgaccatt tcagaagcgt ttggttttga
     9781 tttgtcccta tttttttaaa gaaagaaaaa agatatgaat gatttactag caacagtcat
     9841 tactgggctt attaaagaag aaaacgctaa tgactatttt atccaaaaag aggggtttac
     9901 ctttaccttg tctaaagcag aaggagaacg ccagattggt gatatggtca caggctttgc
     9961 ctatacagac ataaagcaaa aggcacgtct cacaaccaaa gagatccgtt caacacggac
    10021 aagttatggc tggggggaag tgacagaggt tcgtcgtgac ctaggcgttt ttgtcgatac
    10081 aggaattcca aataaagaaa ttgtggtttc tttggacgtg ttgcctgaaa tgaaagaact
    10141 atggcctaaa aaaggggata aactttacat tcgtttggat gtggataaaa aagaccgtat
    10201 ttggggactt ccagcagagc ctgaggtctt tcaaaagatg gctagccctg tatacaataa
    10261 tatgcaaaat caacattggc cagcaattgt ttaccgccta aaattaacag gaacgtttgt
    10321 ttacctgcct gaaaacaaca tgttaggctt tattcaccca agtgaacgct acgcagaacc
    10381 gcgcttaggt caggttttag atgtccgtgt gattgggttt agagaggtcg atcgtaccct
    10441 aaacttgtcg ttgaaaccac gttcttttga gatgttggag aatgacgctc agatgattgt
    10501 cacttatctt gaagccaatg gtggcttcat gaccttaaat gacaaatcaa gtccagaaga
    10561 gattaaagca acctttggta tttcaaaagg ccagtttaaa aaagccttgg gtggattgat
    10621 gaaagctaaa cgcatcaaac aagacgccac aggaactgaa ttaataggat aaaaatgctt
    10681 tgccactggc aaagtttttt gttacaatga agaccattaa gtgaagaaaa aggggatata
    10741 gagaatggaa agagccattt ttgcaggagg ttgtttctgg tgtatggtgc aaccttttga
    10801 ggagcaagca ggtattttgt cagtcagaag tggctacaca ggtgggcatc tcccaaatcc
    10861 tagctatgaa caagtttgtg ccaaaacaac aggacataca gaagctgttg aaattatttt
    10921 tgaccctgaa gagatcagct ataaggaatt agttgaattg tattgggtgc agacggatcc
    10981 aacagacgcc tttgggcaat ttgaagatcg tggggacaac taccgtcctg tcatttacta
    11041 cacaacagaa agacaaaaag aaattgcaga gcaatcaaaa gcaaacttgc aagcttcagg
    11101 acgttttgac caacccattg tcaccactat tgaaccagca gagccatttt atctcgcaga
    11161 ggattatcac caaggatttt ataaaaagaa tcccaggcgg tatgcacaaa gcagtgctat
    11221 tcgtcatcag tttttagagg agaattggtc atgagaaaat cattttacag ctggctgatg
    11281 acacaacgta accctaaatc aaatgcacct gtggcgattt tagcagattt ggtgtttgat
    11341 gacaccactt ttccaaagca taccaatgat tttgaactca ttagtcggta tctagaagat
    11401 caagctagtt tttcctttaa tttaggtcaa tttgatgaaa tctgggaaga ttatctagca
    11461 cattaatacg tggataagaa agagtcatat cgactctttt gaagggttat cacgttatta
    11521 ttacaaaatg acatcacttt ctttcaaaaa aatgacaagc tacttttttt cttaaaaata
    11581 tgctatgata tagcttagaa aaaaataaaa aggaagcaga gatgattatt actaaaaaga
    11641 gtttatttgt gacaagtgtc gctttgtcgt tagcaccttt ggcgacagcg caggcacaag
    11701 agtggacacc acgatcggtt acacaaatca agtctgaact cgtcctagtt gataatgttt
    11761 ttacttatac agtaaaatac ggtgacactt taagcacaat tgctgaagca atggggattg
    11821 atgtgcatgt cttaggagat attaatcata ttgctaatat tgacttaatt tttccagaca
    11881 cgatcctaac agcaaactac aaccaacacg gtcaggcaac gactttgacg gttcaagcgc
    11941 ctgcttctag tccagctagc gttagtcatg tacctagcag tgagccatta ccccaagcat
    12001 ctgccacctc tcaatcgact attcctatgg caccatctgc gacaccatct gatgtcccaa
    12061 cgacaccatt agcatctgca aagccagata gttttgtgac agcgtcatct gagctcacat
    12121 catcaacgaa tgatgtttcg actgagttgt ctagcgaatc acaaaagcag ccagaagtac
    12181 cacaagaagc agttccaact cctaaagcag ctgaaacgac tgaagtcgaa cctaagacag
    12241 acatctcaga agattcaact tcagctaata ggcctgtacc taacgagagt gcttcagaag
    12301 aagcttcttc tgcggcccca gcacaagctc cagcagaaaa agaagaaacc tctcagatgt
    12361 taactgcgcc agcagcacaa aaagctgtag ctgacaccac aagtgttgca acctcaaacg
    12421 gcctttctta cgctccaaac catgcctaca atccaatgaa tgcagggctt caaccacaaa
    12481 cagcagcctt caaagaagaa gtggcttctg cctttggtat tacgtcattt agtggttacc
    12541 gtccaggaga tccaggagat catggtaaag gattagccat tgactttatg gtaccggtta
    12601 gctctacgct tggtgatcaa gttgctcaat atgccattga ccatatggca gagcgtggta
    12661 tttcatacgt tatttggaaa cagcgattct atgcgccatt tgcaagtatt tacggaccag
    12721 cctacacatg gaaccccatg ccagatcgcg gcagtattac agaaaaccat tatgatcatg
    12781 ttcatgtctc ctttaatgct taattcatgc tatcaaaaaa actcacgttt aggcgtgagt
    12841 tttttgacaa ttctgacaga gtaagcccta taagaggctg agatacgctt ttaatacctg
    12901 ttgtttttct cgccctttag acagttttaa cgttatgtct aaaagagatg gtttttatta
    12961 gcgcaaatat gggtttaggg gtaaaatata gataattaac ctgatatgat catgataaag
    13021 gtagaagaat acctattatt tcgtgacaac tgagcaatct gcccttacct ttggagtgat
    13081 gatatcactt tgcttgacta ttcaaggagg tcttgaaatg tattatacta gtggtaatta
    13141 cgaagctttt gcgacacctc gaaaacctga aggggtagat cagaaatcgg cttatattgt
    13201 tggtactggt ttagctggtt tagcagcagc tgttttcctt attcgcgatg ggcatatggc
    13261 tggggaacgc attcatctgt ttgaggaatt gcctttagca ggtggttctt tagatggtat
    13321 tgaaaagcct catcttggtt ttgtgacccg tggtggtcgt gagatggaaa atcattttga
    13381 gtgtatgtgg gacatgtatc ggtctattcc ctcactggaa attcctggtg cgtcttattt
    13441 ggatgaattt tattggttgg ataaggatga tcctaactca tccaactgtc gtttgattca
    13501 caagagagga aatcgtgtgg atgatgacgg ccagtatacg ctcggtaaac agtcaaaaga
    13561 attagtccat ttaatcatga agacagaaga atctctagga gatcaaacca ttgaagagtt
    13621 cttctcagaa gatttcttta agagtaattt ttggatctat tgggcaacca tgtttgcttt
    13681 tgaaaaatgg cattctgctg tagaaatgcg gcgctatgcg atgaggttta tccaccatat
    13741 tgatggtttg ccagatttca cctctctcaa gttcaacaaa tataaccaat atgattctat
    13801 ggtcaaaccg attattgctt acctagaatc acacgacgtt gacatccaat ttgacacaaa
    13861 agtcactgat attcaggtgg aacaaacagc tggtaaaaag gtagcaaaaa ccatccatat
    13921 gacggtgtct ggggaggcta aggcgattga gctaacacct gatgatttgg tttttgtgac
    13981 caatggttct attactgaaa gtagcacata cggtagtcat cacgaagtgg ctaagccaac
    14041 caaagcgtta ggtggttctt ggaatttatg ggaaaatcta gctgctcaat cagatgattt
    14101 tggtcatcct aaagtgtttt accaggactt gcctgctgaa agctggtttg tgtctgccac
    14161 agcaaccata aaacacccag ctatcgagcc ttatattgaa cgtttgaccc accgtgattt
    14221 gcacgatggc aaagtgaaca ctggcggcat catcactatt acagattcta actggatgat
    14281 gagctttgcc attcaccgtc aacctcattt taaagaacaa aaagataatg aaaccactgt
    14341 ctggatttac ggtctttatt ccaatagtga gggcaattac gtccacaaga aaattgagga
    14401 gtgtacaggt caagaaatca cagaagaatg gttgtaccac cttggggtac ctgttgataa
    14461 aatcaaggac ttagcgagtc aggactatat caatacagtt cctgtttaca tgccttatat
    14521 tacgagttac tttatgccac gtgtcaaagg agaccgtccg aaagttatcc cagatggttc
    14581 agtcaacttg gcctttattg gtaactttgc ggaatctcca tctcgagata cggtctttac
    14641 gactgagtat tctattcgta ctgccatgga agcagtgtat agcttcttga atgtggaacg
    14701 aggcattcca gaagtcttta attcagccta tgatattcgt gaattgctca aagcctttta
    14761 ttaccttaat gataaaaagg caatcaagga tatggatttg ccaattcctg cactgattga
    14821 gaaaatcgga cataaaaaaa tcaaggatac ctttatcgaa gaattgctca aagatgctaa
    14881 tcttatgtaa catgccttag agtagttaaa gctgataagt ttaggcttat cagctttttt
    14941 gatgatttat agaaaagtga taaaggaaaa gtctgacgtg aacgtcacat aagaatttaa
    15001 aatttgttat aatgtaagca ataaagatga aagaaagagg gactctattt gcaagagtat
    15061 tctattgaca ttaccttaac ccatcctgat gatgttttag ctctttttgg tagcaacgag
    15121 cgtcatttga agctaataga agctcattta ggagttattg ttcacgcaag aaccgagcga
    15181 gtccaagtga ttggtgatga tgaagaagct gtggaattgg ctcgattgac catcaaagcc
    15241 ctactggttc tagtgggtcg tggcatggtg gtcaacacat cagatgtggt gacagcttta
    15301 tctatggcag aatctcatca gattgaccaa tttatggcgc tttatgaaga agaaatcatt
    15361 aaagataact atggtaaggc aatacgagtc aaaacattag gtcaaaaaac ctatgttgat
    15421 agtgtcaaac gtcatgatgt ggtttttggt gtaggacctg cagggacagg gaaaaccttc
    15481 ttagctgtta cgcttgcagt cactgctctg aaaagaggac aggtaaagcg tattattttg
    15541 acgcgtcctg cggtagaagc tggtgaaagc ctaggttttt taccaggcga tttgaaagaa
    15601 aaggtggatc cttacctcag acctgtttac gatgcccttt atcacatttt aggcaaggaa
    15661 caaaccacac gcctgatgga acgagatgtg attgagattg ctccgttagc ttatatgcgg
    15721 ggtcgtactc tggatgacgc ttttgttatc ttagatgagg ctcaaaacac gaccatcatg
    15781 caaatgaaga tgtttttgac ccgtcttggt tttaattcta aaatgattgt caatggagat
    15841 acgagtcaga ttgacctccc tcgaaatgtc aagtctggtt tgattgatgc tactcagaaa
    15901 ttacaaggca ttaaacagat tgattttgtc tatttctctg ctaaggatgt tgtccgacat
    15961 cctgttgtgg ctgatattat caaggcttat gaaacgtcct cagaggagat gaaggactta
    16021 ctaaaaccta aggctagtga cgttgaagct gattaccagc caggcttgac aaaataccct
    16081 gtgattggtc aagagcacta agatttttac gatgatgttt aatatggcct ttttaaggag
    16141 gattctatgg atgatttaac caaagctatt atggcagatg aggctaatct ctcctatacc
    16201 gaaagaggta tttttccttt atatgatgct cctcaaacag ctaggcttat tattgttggc
    16261 caagctcctg gaatagtagc tcaagaaacc aaactttact ggaatgatcg tagtggcatc
    16321 cgcttacgag actggctagg tgtggacaat gacacctttt accactcagg attgtttggc
    16381 attatcccta tggatttcta ttatcctggt aagggaaaat ccggtgactt accacctaga
    16441 gaaggttttg cggctaagtg gcacccgcca ttaagagcat tgatgccaga agttgaattg
    16501 acgattttgg ttggtcgcta tgctcaggac ttttaccttg gtaacaaggc ttacaaaacc
    16561 ttaactgaaa cagtccgtca ctttgaggat tacttgccag actattttcc acttgttcat
    16621 ccttctccaa gaaaccaatt gtggttagct aagaacccct ggtttgagca ggatttatta
    16681 cctatcttgc aaaaacgtgt ggaggcaatt ctaaccaaat aggccctgat ttacctagca
    16741 ttcccatttt ttaggggctt gtggtataat gggtaaataa caaatgctga ttgaaaggat
    16801 aagggaaatt atctgtcaga taataatggg tgcccaacat accaaatgag gtgggcagcc
    16861 cttccctaac aattaacctt atgtatatcg agatgattga cgaaacggga caagtttcgc
    16921 aagagattat ggagcaaaca cttgatttgc tcaattttgc tgctcaaaag acgggcaaag
    16981 aagaaaaaga aatgtctgtg acctttgtga ccaatgaaag aagtcatgaa ctcaacttag
    17041 aataccgaga tactgatcgt ccaacagatg tgatttctct agaatataag ccagaaacgc
    17101 ctattttatt tagtcaagag gattttgctg ctgatcctag tttggcagag atgatggcag
    17161 agtttgatgc ttatattggc gaattgttta tttctattga caaggcgcgt gagcagtccc
    17221 aagaatatgg gcactctttt gagcgtgaaa tgggattttt agctgttcat ggattcttac
    17281 atattaatgg gtatgatcac tacacccttg aagaagaaaa agagatgttt accttacagg
    17341 aagagatttt gactgcttat ggccttacac gacaataata ccaccaaacg gaaatggaaa
    17401 aacagaacca ttacctcaag cctggagttt gccttgacag gggttttcac agcttttaag
    17461 gaagaacgca atctcagaag tcaccttttg tctgcctgtt tagcttgtgt tgctggttta
    17521 ttttttagca tttcagccat cgaatggtta tttttacttc tagccatttt tttggtcatt
    17581 accttagaaa ttgtcaattc tgctattgaa aatgtagttg atttggctag tgactatcac
    17641 ttttctatgc tagctaaaaa tgccaaggat atggcagcag gtgctgtttt gatgatttct
    17701 ggctatgctg tcttgacagg acttattatt tttattccta aaatttggaa tattttcgtt
    17761 cattaaaaag aaaggaatgg gctatcctat gtgatgatgg cataggagaa taaggaaagg
    17821 acaagacggt taagcttagt gacaagcttg atctgaagcc ctaccgttaa ggcccgtaat
    17881 tatctatgtt taaatcaggc tttgtagcca ttcttggtcg tccaaatgtt ggcaaatcca
    17941 cctttttaaa tcacgtcatg gggcaaaaaa ttgccatcat gagtgacaag gctcagacca
    18001 cccgtaataa aattatgggc atttacacca cagaaacaga acaaattgtc tttattgata
    18061 cgccaggtat tcataaacca aagaccgccc ttggtgattt tatggttgaa tctgcttata
    18121 gtactttacg tgaagttgag acggttcttt tcatggttcc tgccgatgaa aagcgtggta
    18181 agggtgacga catgattatc gagcggctaa aagcagctaa gattcctgtt attttagtta
    18241 tcaacaaaat tgataaggtt catccagacc aacttttgga acagattgat gacttccgta
    18301 gccaaatgga ttttaaagaa gtggtcccaa tttctgccct agaaggtaat aatgtgccaa
    18361 ccctaatcaa gttattaact gataatcttg aagaaggttt ccaatatttt ccagaagatc
    18421 agattactga tcatccagaa cgtttccttg tgtcagaaat ggttcgtgag aaggtactgc
    18481 atttgacgca acaagaggtt ccacattccg tagctgttgt tgtcgaatcc atgaagcgag
    18541 atgaagagac tgataaagtg catatccgtg caaccattat ggtggagcgc gatagccaaa
    18601 aaggcatcat cattggaaaa caaggagcca tgctcaaaaa aatcggaaaa atggcgcgcc
    18661 gtgatattga actcatgtta ggtgacaagg tctacttaga aacgtgggtc aaagtcaaga
    18721 aaaactggcg ggataaaaag cttgatttag ctgattttgg ctataatgag aaagaatact
    18781 aacatagtaa agcgagatca tatgccacaa gactacattt cttatatccg ctccaaagtt
    18841 gggcatgata agattattct taactttgct ggtgggattt tgaccaatga tgacggcaag
    18901 gtgctgatgc agttgcgtgg tgataagaaa acctgggcta ttcctggtgg tactatggaa
    18961 ctgggtgaga gctctttaga gacttgcaaa cgtgagttct tagaggaaac cggaattgag
    19021 gttgaagctg ttcgcttact gaatgtttac actcattttg aggaagttta tcccaacggt
    19081 gatgctgttc agactattgt ctttatctat gaactaacgg ctgtttctga tatggctatt
    19141 gataactttc ataatgagga aacgctcaag ttacagtttt tctctcatga ggagatagca
    19201 gagttagaga gtgtctcagc caaacatcgc ctgatgttag aggaatattt tagtgatagc
    19261 tttgctatgg ggcattaaga actctctagg acagttggag tatttttgga gattggcttg
    19321 ttaaaaaaat ttttgaaaga cgatgtttcg agagcttatg ctctcttttt ttgcttccaa
    19381 tttttctgat taagtctgaa taattttgtt aaatgttaac aggattttct ttccaattga
    19441 tactagcaga catcaaacat ttgttttaga gttatgacga attgccacct agccatgcat
    19501 gacttaggca agttgaacct aactgtggca atagacttgg aaggggtgac aagaatcatt
    19561 tcctaaaaga attgatgaag tgcctatcca ttattcgaat tgtaaagttc gtctaagtaa
    19621 agttgaattt gatctcgaat ggactttaat gatttatgac ttgttgtgtc aataacacca
    19681 aaaactcgat aatgattttt aaatatttcc tcgtaattaa tatgatttcc gattagaata
    19741 atatgagcat gaggataacg catacgtatt tcgtttagaa ggtcatatac ttgctc
//
LOCUS       DATXQK010000030        18282 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.030, whole genome shotgun sequence.
ACCESSION   DATXQK010000030 DATXQK010000000
VERSION     DATXQK010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18282
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.030"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            431..633
                     /locus_tag="VC394_001166"
                     /pseudo
     CDS             431..633
                     /locus_tag="VC394_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051153.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
     gene            835..1215
                     /locus_tag="VC394_001167"
     CDS             835..1215
                     /locus_tag="VC394_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEP5994639.1"
                     /translation="MQLVREKEFVNQYHYDARNLEWEKENGTPETNFEVTFQLIDKDE
                     QQKETVIVSVLQFVIVKEEFVISGVISQMVRILDRLVDKPSEFTQEEVESLAAPLLDM
                     VKRLTYEVTEIALDRPGIHLEFKN"
     gene            1215..2063
                     /locus_tag="VC394_001168"
     CDS             1215..2063
                     /locus_tag="VC394_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEP5994640.1"
                     /translation="MKLAVITDSTATLPTDLKQDKAIFSLDIPVIIDDETYFEGRNLS
                     IDDFYQKMADSQNLPKTSQPSLSELDNLLGLLSSKGYTHVIGLFLAGGISGFWQNIQF
                     LAEEHPEIEMAFPDSKITSAPLGSMVKNVLDWSRQGMTFQAILNKLQEQIDGTTAFIM
                     VDDLNHLVKGGRLSNGSALLGNLLSIKPILRFDEEGKIVVYEKVRTEKKAMKRLVEIL
                     NDLIADGQYNVSIIHSKAQDKADYLKRLLQDSGYQYDIEEVHFGAVIATHLGEGAIAF
                     GVTPRL"
     gene            2188..3396
                     /locus_tag="VC394_001169"
     CDS             2188..3396
                     /locus_tag="VC394_001169"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEP5994641.1"
                     /translation="MKLMTMPSEFQKALPILTKIKEAGYEAYFVGGSVRDVLLERPIH
                     DVDIATSSYPEETKAIFNRTVDVGIEHGTVLVLENGGEYEITTFRTEDVYVDYRRPSQ
                     VSFVRSLEEDLKRRDFTVNALALDENGQVIDKFRGLIDLEQKRLRAVGKAEERFEEDA
                     LRIMRGFRFAASLDFDIEAATFEAMRSHSPLLEKISVERSFTEFDKLLMAPHWRKGIS
                     AMIACQAYDYLPGLKQQEAGLNHLIVSLKDNFTFSDHHQAWAYVMISLAIEDPKSFLK
                     AWKTSNDFQRYVTKLIALYRIRQERSFEKLDIYQYGKEMASLVEGLRKAQSLSVDMDH
                     IEALDQALAIHNKYDIVLNGSHLIKDFGMKPGPQLGLMLEKVELAIVEGRLDNDFTTI
                     EAFVREELAT"
     gene            3393..5270
                     /locus_tag="VC394_001170"
     CDS             3393..5270
                     /locus_tag="VC394_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEP5994642.1"
                     /translation="MSHFLVKKLTKTVGDKTVFQDISFIIHDFDRIGIIGVNGTGKTT
                     LLDVLSGRLGFDGDHSPFSKANDYKIAYLTQDPEFNDASSVLDTVLSSDVKAIQLIRQ
                     YELLMANYTEDKQETLESLMSEMDRLDAWSIESDVKTVLSKLGITDLEQKVGDLSGGM
                     RRRVQLAQVLLGAADLLLLDEPTNHLDIDTIAWLTTYLKTAKKTVLFITHDRYFLDHV
                     ATRIFELDKAGLTEYQGNYQDYVRLKAEQDERDATNLHKKKQLYKQELAWMRTQPQAR
                     ATKQQARINRFTDLKKEVHQDSSADKLEMAFETSRIGKKVIHFEDLSFAYGDRQLIKD
                     FNLIIQNKDRIGIVGDNGVGKSTLLNIINGDLKPTSGKLDIGDTIRVGYFSQQLKDMD
                     ETKRVINYLQEVADEVKTSVGTTSISELLEQFLFPRSSHGTLIAKLSGGEKKRLYLLK
                     LLIEKPNVLLLDEPTNDLDIATLTVLENFLANFAGPVITVSHDRYFLDKVATKILAFE
                     EGDIRIFYGSYSDYLDEKAFEKETVEADLAKAIVTEEVPLPPKEEKKRMSYLEKQEWA
                     QIEDKIATIEANIEEIENQMLTVVSDYGQLAQLQKELDQRNNDLLLAYERFEYLSGLD
                     I"
     gene            complement(5368..5445)
                     /locus_tag="VC394_001171"
                     /pseudo
     CDS             complement(5368..5445)
                     /locus_tag="VC394_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            6162..6572
                     /locus_tag="VC394_001172"
     CDS             6162..6572
                     /locus_tag="VC394_001172"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEP5994643.1"
                     /translation="MIREIITDHFLLQQKAQVAKKEDLWIGQDLQDTLAFYRQECLGM
                     AANMIGEQKRIVIVSMGFIDLVMFNPVMVSKKGIYQTKESCLSLSGYRKTQRYDKITV
                     EYLDHNWRPKRLSLTGLTAQICQHELDHLEGILI"
     gene            complement(6655..8667)
                     /locus_tag="VC394_001173"
     CDS             complement(6655..8667)
                     /locus_tag="VC394_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEP5994644.1"
                     /translation="MKKYFILKSSVLSILTSFTLLVTDVQADQVDVQFLGVNDFHGAL
                     DNTGTAYTPSGKIPNAGTAAQLGAYMDDAEIDFKQANQDGTSIRVQAGDMVGASPANS
                     ALLQDEPTVKVFNKMKFEYGTLGNHEFDEGLDEFNRIMTGQAPDPESTINDITKQYEH
                     EASHQTIVIANVIDKKTNDIPYGWKPYAIKDIAINDKIVKIGFIGVVTTEIPNLVLKQ
                     NYEHYQFLDAAETIAKYAKELQEQHVHAIVVLAHIPATSKDGVVDHEMATVMEKVNQI
                     YPEHSIDIIFAGHNHQYTNGTIGKTRIVQALSQGKAYADVRGTLDTDTNDFMTTPSAN
                     VIAVAPGIKTENSDIKAIINHANDIVKTVTERKIGTATNSSTISKTENIDKESPVGNL
                     VTTAQLTIAKKTFPTVDFAMTNNGGIRSDLVVKNDRTITWGAAQAVQPFGNILQVIQM
                     TGQHIYDVLNQQYDENQTYFLQMSGLTYTYTDNDPKNSDTPFKIVKVYKDNGEEINLT
                     TTYTVVVNDFLYGGGDGFSAFKKAKLIGAINTDTEAFITYITNLEASGKTVNATIKGV
                     KNYVTSNLESSTKVNSAGKHSIISKVFRNRDGNIVSSEIISDLLTSTENTNNSLGKKE
                     TTTNKNTISSSTLPITGDNYKMSPIMTILALISLGGLNAFIKKRKS"
     gene            8887..9537
                     /locus_tag="VC394_001174"
     CDS             8887..9537
                     /locus_tag="VC394_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEP5994645.1"
                     /translation="MKERLEHYSTSSIYSGFEVYLPLVLQTITDVIIAENIKSKKETG
                     FKLYEHFTSRIKSEASMIEKCQRKQLPLTSKSALKIIKDSIGIRIICGFIDDIYRMVD
                     LLKSIPGMSVNTEKDYILNAKPNGYRSYHLILELETHFPDILGEKKGCYFIEVQLRTI
                     AQDSWASLEHQMKYKHQVANAEMITRELKRCADELASCDVTMQTIRQLIQETTEEE"
     gene            9540..10208
                     /locus_tag="VC394_001175"
     CDS             9540..10208
                     /locus_tag="VC394_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP5994646.1"
                     /translation="MKILLAEDEWQMSNVLTTAMTHQGYDVDVVFNGQEAIDKAKDNA
                     YDIMILDIMMPIKSGIEALKEIRASGNCSHIIMLTAMAEINDRVTGLDAGADDYLTKP
                     FSLKELLARLRSMERRVESFTPQVLQFAGVTLNINEQELSAGNAIRLASKEGKLMAFL
                     MLNQGKYLDTKTLYQHVWSDQEDYDTSIVWVYISYLRQKLLAIQANVIIAGDKDTSYC
                     LLEK"
     gene            10217..11449
                     /locus_tag="VC394_001176"
     CDS             10217..11449
                     /locus_tag="VC394_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEP5994647.1"
                     /translation="MFNRIRIRFIMIASIAIFIILSSIVGIINTARCYQSQQEINRIL
                     HLISSNKGKLPGTTESSKRLGTKLSEDSLSQFRYYSVIFNANGHLLSSNTANISALDR
                     EEAQYFARLFAKSGEEKGSYRHQDSVYSYLITQLPNEEKLVVILDTTFYFRSVGDLLA
                     VSVMLAFGGFIFFVVLVSLFSGMVIKPFVQNYEKQRRFITNAGHELKTPLAIISANNE
                     LVELMTGESEWTKSTSDQVKRLTGLINQMITLARLEEQPDVVLHMVDFSAIAQDAAED
                     FKSLVLKDGKRFDLTIQPNIMIKAEEKSLFELVTILVDNANKYCDPKGLVKVSLTTIG
                     RRRKRAKLEVSNTYLEGKSIDYSRFFERFYREDESHNSKEKGYGIGLSMAESMVKLFK
                     GTITVNYKNDAIVFTVVI"
     gene            11742..12620
                     /gene="mvk"
                     /locus_tag="VC394_001177"
     CDS             11742..12620
                     /gene="mvk"
                     /locus_tag="VC394_001177"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEP5994648.1"
                     /translation="MNENIGYGKAHSKIILIGEHAVVYGYPAIALPLTDIEVVCHIFP
                     ADKPLVFDFYDTLSTAIYAALDYLQRLQEPIAYEIVSQVPQKRGMGSSAAVSIAAIRA
                     VFSYCQEPLSDDLLEILVNKAEIIAHTNPSGLDAKTCLSDHAIKFIRNIGFETIEIAL
                     NGYLVIADTGIHGHTREAVNKVAQFEETNLPYLAKLGALTQALERAINQKNKVAIGQL
                     MTQAHSALKAIGVSISKADQLVEAALRAGALGAKMTGGGLGGCMIALADTKDMAEKIS
                     HRLKEEGAVNTWIQML"
     gene            12602..13546
                     /gene="mvaD"
                     /locus_tag="VC394_001178"
     CDS             12602..13546
                     /gene="mvaD"
                     /locus_tag="VC394_001178"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEP5994649.1"
                     /translation="MDPNVITVTSYANIAIIKYWGKENQAKMIPSTSSISLTLENMFT
                     TTSVSFLPDTATSDQFYINGVLQNDEEHTKISTIIDQFRQPGQAFVKMETQNNMPTAA
                     GLSSSSSGLSALVKACDQLFDTQLDQKALAQKAKFASGSSSRSFFGPVAAWDKDSGAI
                     YKVETDLKMAMIMLVLNAAKKPISSREGMKLCRDTSTTFDEWVEQSAIDYQHMLTYLK
                     TNNFEKVGQLTEANALAMHATTKTANPPFSYLTKESYQAMEAVKELRQEGFACYFTMD
                     AGPNVKVLCLEKDLAQLAERLGKNYRIIVSKTKDLPDV"
     gene            13539..14552
                     /locus_tag="VC394_001179"
     CDS             13539..14552
                     /locus_tag="VC394_001179"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEP5994650.1"
                     /translation="MSNYCVQTGGKLYLTGEYAILTPGQKALIHFIPLMMTAEISPAA
                     HIQLASDMFSHKAGMTPDASYALIQATVKTFADYLGQSIDQLEPFSLIITGKMERDGK
                     KFGIGSSGSVTLLTLKALSAYYQITLTPELLFKLAAYTLLKQGDNGSMGDIACIAYQT
                     LVAYTSFDREQVSNWLQTMPLKKLLVKDWGYHIQVIQPALPCDFLVGWTKIPAISRQM
                     IQQVTASITPAFLRTSYQLTQSAMVALQEGHKEELKKSLAGASHLLKELHSAIYHPKL
                     VTLVAACQKQDAVAKSSGSGGGDCGIALAFNQNARDTLISKWQEADIALLYQERWEEN
                     DQP"
     gene            14539..15528
                     /locus_tag="VC394_001180"
     CDS             14539..15528
                     /locus_tag="VC394_001180"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEP5994651.1"
                     /translation="MTNRKDDHIKYALKYQSPYNAFDDIELIHHSLPSYDLSDIDLST
                     HFAGQDFDFPFYINAMTGGSQKGKAVNEKLAKVAAATGIVMVTGSYSAALKNPNDDSY
                     RLHEVADNLKLATNIGLDKPVALGQQTVQEMQPLFLQVHVNVMQELLMPEGERVFHTW
                     KKHLAEYASQIPVPVILKEVGFGMDVNSIKLAHDLGIQTFDISGRGGTSFAYIENQRG
                     GDRSYLNDWGQTTVQCLLNAQGLMDQVEILASGGVRHPLDMIKCFVLGARAVGLSRTV
                     LELVEKYPTERVIAIVNGWKEELKIIMCALDCKTIKELKGVDYLLYGRLQQVN"
     gene            complement(15758..17035)
                     /locus_tag="VC394_001181"
     CDS             complement(15758..17035)
                     /locus_tag="VC394_001181"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEP5994652.1"
                     /translation="MTKTNLNWSGFSKKTFEERLQLIEKFKLLNAENLNQLKTDVLLP
                     IQTANQMTENVLGRLALPFSIAPDFLVNGSTYQMPFVTEEPSVVAAASFAAKLIKRSG
                     GFKAQTLNRQMIGQIVLYDIDQIDNAKAAILHKTKKLIALANKAYPSIVKRGGGARTI
                     HLEEKGEFLIFYLTVDTQEAMGANMVNTMMEALVPDLTRLSKGHCLMAILSNYATESL
                     VTTSCEIPVRLLDHDKTKSLQLAQKIELASRLAQVDPYRATTHNKGIFNGIDAVVIAT
                     GNDWRAIEAGAHAYASRNGSYQGLSQWHFDQDKQVLLGQMTLPMPIASKGGSIGLNPT
                     VSIAHDLLNQPDAKTLAQLIASVGLAQNFAALKALTSSGIQTGHMKLHAKSLALLAGA
                     TQDEIAPLVNALLADKPINLEKAHFYLSQLRQS"
     gene            complement(17022..18197)
                     /locus_tag="VC394_001182"
     CDS             complement(17022..18197)
                     /locus_tag="VC394_001182"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEP5994653.1"
                     /translation="MTIGIDKIGFATSQYVLKLEDLALARQVDPAKFSQGLLIESFSV
                     APITEDIITLAASAADQILTDEDRSKIDMVILATESSTDQSKASAIYVHHLVGIQPFA
                     RSFEVKQACYSATAALDYAKLHVASKPDSRVLVIASDIARYGVGSPGESTQGSGSIAL
                     LVTANPRILALNEDNVAQTRDIMDFWRPNYSFTPYVDGIYSTKQYLNCLETTWQAYQK
                     RENLQLSDFAAVCFHIPFPKLALKGLNNIMDNTVPPEHREKLIEAFQASITYSKQIGN
                     IYTGSLYLGLLSLLENSKVLQSGDKIGFFSYGSGAVSEFYSGQLVAGYDKMLMTNRQA
                     LLDQRTRLSVSKYEDLFYEQVQLDDNGNANFDIYLTGKFALTAIKEHRRIYHTNDKN"
BASE COUNT         5808 a         3113 c         3724 g         5637 t
ORIGIN      
        1 aaaataatac ttaatttttt aaataatttt taataaacgt aaacaagcga ttgacaatgg
       61 agatatcaca agagatacag tagtggttgt gcttaaaatg gacggttttt tgatgatatg
      121 ttgccgcatg aggaagtgag caataggaga gctgtgatag aagaaagaga ggaagaagcg
      181 ttgatgaaat tagacaaata aaaaaaaaga cctcatccag aagaggtcgt ggtataactg
      241 gggacgatag gttcaattga taaaaaatct tgttagctta cgatctatgt tgttttgaat
      301 gcctttaact caacatcgtt aatggatttt gagataaaaa aataaatcca atagtctggt
      361 actttcctat tttttgtctg ttaaaacgtt tttattttat aatggataaa aggagaagaa
      421 gggcggagcg atgattaagc tctatgggtt taagtatggg ttagttggag aggttttgcg
      481 gcggattgaa cgcctaggat ttacgtttga gcgattagag ctaagacaag ctagttcgaa
      541 atacttggct aagcatgatg aggccttggt gataaacctt tttatcctgg acttaaaact
      601 tacctgacca gtggttctgt tttaattgag gtgcagtaca agaccactaa cgggtgtttt
      661 tttgatataa tgtcagtata aggacgacta aatataaata ggtgaaaaaa tagatatctg
      721 ttcgagagat agcactgttt aaaacgttat gcgaaaatag tagtttaggg gctactttaa
      781 taatcttcaa atctatgcta taatggatca tattgatacc aaggagaata aaaaatgcaa
      841 ttagttcgtg aaaaagaatt tgtcaaccag taccattatg atgctagaaa tttagagtgg
      901 gaaaaagaaa acggaacacc agagaccaat tttgaagtga cttttcagtt gattgataaa
      961 gatgagcagc aaaaagaaac agtaattgta tctgtattgc aatttgttat tgtcaaagaa
     1021 gaatttgtta ttagtggggt catctcacaa atggttcgta ttttagatcg tttagtggat
     1081 aaaccaagcg aatttacgca agaggaagtt gaatccttag ctgctccttt gctagacatg
     1141 gttaagcgat tgacttatga agtcacagaa attgccttgg atcgtccagg tattcatttg
     1201 gagtttaaaa attaatgaaa ttagcagtaa ttactgacag tacagcaaca ctaccaacag
     1261 atctgaaaca agataaagct atttttagcc ttgatatacc agttatcatt gacgatgaga
     1321 cctattttga aggtcgaaat ttgagtatag atgattttta tcaaaagatg gctgactccc
     1381 aaaatcttcc caagacaagt cagccaagtt tatctgaact agataatctt ttaggacttt
     1441 tgtcttctaa gggctataca catgttattg gcttgttctt agctggagga atctcaggct
     1501 tttggcaaaa tattcaattt ttagctgaag aacatcctga gattgaaatg gcatttccag
     1561 acagtaagat cacatctgct cctttaggta gtatggtaaa aaatgtgcta gattggtcac
     1621 gtcaaggaat gacatttcaa gctattttaa acaagctgca agaacagatt gatgggacga
     1681 cagcctttat tatggttgat gaccttaatc atctggttaa aggcggacgc ctgtcaaatg
     1741 ggtctgctct tcttggaaat cttttgagta ttaaacctat cttacgcttt gatgaagagg
     1801 gaaaaatcgt tgtttatgaa aaagtgagaa cagaaaagaa agccatgaaa cgcttggttg
     1861 agattttaaa tgacttgata gcagacggtc agtataatgt gtctattatt cactcaaaag
     1921 cacaagacaa agctgattat ttgaaacgac tcttacaaga cagtggttat caatatgaca
     1981 ttgaagaggt tcattttgga gcggtgattg ctactcattt aggagaagga gccattgcat
     2041 ttggcgtaac accgaggctt taattgtcga ttagaaaaaa tagttaattg caagaatcac
     2101 taaaacaata acaaaatgtg atagaaccca agtagcctaa tcacacttta gattgggcta
     2161 cttggatgat aggttagaaa acaacgtatg aaattaatga caatgccttc tgagtttcag
     2221 aaggctttac ccatattgac taaaataaaa gaagctggtt atgaagccta ctttgtggga
     2281 ggaagcgtca gagatgtttt actcgagcgt cccattcatg atgtagatat tgcaactagt
     2341 tcctatcctg aagagactaa agcgattttt aacagaactg tcgatgttgg cattgaacat
     2401 ggtacagtgc tcgtgcttga aaatggcggt gagtatgaaa tcacaacctt taggacagaa
     2461 gatgtctatg ttgactatcg ccgtcctagt caggtttctt ttgtgcgctc tttagaggaa
     2521 gacttaaaac gaagagactt taccgtcaat gccttggcct tggatgaaaa tggtcaagtt
     2581 attgataaat ttagaggact cattgatttg gagcaaaaac ggttacgagc agtgggtaaa
     2641 gcagaggaac gttttgaaga agatgcacta cgcattatga gaggctttcg atttgcggcg
     2701 agtctggatt ttgacattga agctgcaact tttgaagcta tgaggagcca ttcacctcta
     2761 ttggaaaaga tttctgtgga acgttctttt actgaatttg ataaattatt gatggcaccg
     2821 cactggcgaa aagggatttc tgccatgatt gcttgtcagg cttatgacta tttaccaggc
     2881 ttgaagcagc aggaagcagg cttgaatcat ctgatagttt ctttgaaaga taactttacc
     2941 tttagtgatc accaccaagc ttgggcttat gtgatgatta gtttagccat tgaggatcct
     3001 aaaagcttcc taaaagcatg gaagacttca aatgattttc agcgttatgt cacaaagttg
     3061 attgctcttt atagaattcg tcaggaaaga tcttttgaaa aattggatat ttaccagtac
     3121 ggcaaggaaa tggcaagtct agtggaaggc ctccgaaaag ctcaaagctt atctgttgac
     3181 atggaccaca ttgaagcttt agatcaagcg ctagccattc ataacaaata cgatatcgtc
     3241 cttaatggtt ctcacctgat taaggatttt gggatgaaac caggtcctca gctagggctt
     3301 atgcttgaga aagttgaatt agcgattgta gagggacgcc tagataatga tttcactaca
     3361 attgaagcat ttgttagaga ggaattagca acatgagcca ttttttggtg aagaaattga
     3421 caaaaacggt gggagataaa acagtctttc aggatatttc ctttattatt catgactttg
     3481 accgtattgg gattattggg gttaatggaa caggaaaaac aactctttta gatgtcttgt
     3541 cgggaaggct tggttttgac ggagaccatt caccattttc taaagccaat gattacaaaa
     3601 tcgcttactt aacacaagat cctgaattta acgatgcatc ctctgtttta gatacggtat
     3661 tgtcatctga tgttaaggcg attcagctga ttcgtcagta tgaactattg atggcgaatt
     3721 atactgaaga taaacaagag accttagagt ccctcatgtc tgaaatggat cgcttagatg
     3781 cttggtctat tgaaagtgat gtcaaaacag tcctatcaaa attaggcatc acggatttgg
     3841 aacaaaaagt gggagaccta tcaggtggta tgagaagacg tgtccagtta gctcaagtat
     3901 tgctaggagc tgctgactta ctcttgttgg atgagccgac caaccatctc gatattgaca
     3961 ccatcgcttg gttaacaacc tatctcaaga cagctaaaaa aaccgtcctt tttattaccc
     4021 atgaccgtta tttccttgat catgtggcta cccgtatttt tgaattggat aaagcgggac
     4081 tgacagaata tcaaggcaat taccaagact atgtgcgctt aaaagcagaa caagacgaac
     4141 gagatgctac taatttacac aagaagaagc aactctataa acaagaatta gcttggatga
     4201 ggacgcagcc acaggcgcgt gcaaccaaac agcaggctag aatcaatcgt ttcactgact
     4261 tgaaaaaaga agttcaccaa gacagttctg cagataaatt ggagatggct ttcgaaacga
     4321 gccgaattgg gaagaaggtg attcactttg aagacctttc ttttgcttat ggcgaccgac
     4381 agttgattaa ggattttaac ttgattattc aaaataaaga tcgtattgga attgttggag
     4441 ataacggcgt cggaaaatct accctgttaa atatcatcaa cggtgatctc aaaccaactt
     4501 ctgggaagct tgatattgga gatactattc gtgtaggtta tttctctcag cagctcaaag
     4561 atatggatga gaccaaacgg gtcattaatt accttcaaga agtggctgat gaggttaaaa
     4621 caagcgtggg aacaacgagt atcagtgagc ttttagagca atttcttttt ccgagatcaa
     4681 gtcatggcac gctgattgct aaattatctg gtggggagaa aaaacggctc tacttgctaa
     4741 aactgctcat tgaaaagcca aatgttcttc tccttgatga gccaacaaat gatttagaca
     4801 ttgccacctt gacggtctta gaaaactttt tagctaactt tgcaggacca gtcattacgg
     4861 ttagtcacga tcgttatttc ctagacaaag tagccactaa gattttagcc tttgaagaag
     4921 gcgatattcg tattttttat gggagttaca gtgattacct tgatgaaaaa gcttttgaaa
     4981 aagaaacagt tgaggctgac ttagctaaag caatagtcac tgaagaagtg ccacttcctc
     5041 cgaaagagga gaaaaagcgc atgtcctacc ttgaaaaaca agaatgggct cagatagaag
     5101 ataaaatagc aactattgag gctaatattg aagaaattga aaaccaaatg ttaactgtag
     5161 ttagtgatta tggccagtta gctcaattac aaaaggaact cgatcagcgc aataatgatt
     5221 tattattggc gtatgaacgg tttgagtatt tgagtggact agacatataa gtatactatg
     5281 gtaatgttat gtttataata aacaagtgat aatagaaaag aagtgctctt gcactgagcc
     5341 ccaaaagttg gctactctat ttgaagtaaa ggatttagtt ctgtattgta gaggattaag
     5401 tccttttcgt tttattttga tacgtttatt gttgcaataa tcgatgataa tagtaggtag
     5461 aacgagtcaa tttagccgtt cttaaaagaa aatctaatca gaactcgtct ttgtccattc
     5521 tttataatgg ttttttgtct tcttctttgc agagatgctt ctttcaaacg gagttccctc
     5581 ggttttttta gcgctaaata tctagcttat cttcatagct taatgtcata caaaaacacc
     5641 ccaaacgtta gattttttta tctaacgttt ggggtgtagt ttacattatg gcgcttcttt
     5701 tctattatta cgtgaggaaa aatcaagtaa aaaagttaag tcaactgaaa aacttgacag
     5761 aaatattcag aaaatttata atactaacta ttaacaacat atcagctatt aaagcatttc
     5821 aaacagcagg tgttttattt ggacctgtta aggctgcaaa tgcaagagaa agcagtatct
     5881 ggtttgaaaa tcttacaaag caataactat ctctcttaga catttgaaga agcagacagt
     5941 aaattcgata atattgtgat gagaacttag caacaatctg ctgatgcagg tcaattttta
     6001 tttggtactc atattgcaga atttcataaa gttgcccaag atatcttgaa ataaggaatc
     6061 acctgatgtt ttaaacagat gatactaagt ctatcatatg gtgataatag tccattgaac
     6121 atgtagttta tgaattaaaa agaaggttta aggagataag aatgattaga gaaatcataa
     6181 cagatcattt tttattacag caaaaagcgc aagtcgccaa gaaagaagat ctatggattg
     6241 gccaagattt acaagatacc ttagcctttt atagacagga atgtcttggg atggctgcca
     6301 atatgattgg tgaacaaaag cgcattgtta ttgtgagtat gggatttatt gatttggtta
     6361 tgtttaatcc tgtgatggtt tctaaaaagg gaatttacca aactaaagaa tcttgtttgt
     6421 cgttatctgg ctatcgcaaa acacagcggt atgataagat tactgttgag tatttggacc
     6481 ataattggcg accaaagcga ttaagcttaa caggcttaac tgctcaaata tgccagcacg
     6541 aattagatca tctagaaggt attttaattt aacaaaaaac ctagagtagg acgctttagg
     6601 ttttttttgg caaagatgcc tgattatata agggtgatcg attactagta aacgctagga
     6661 tttccttttt ttaataaaag cgtttagtcc acctaagctt atcaaggcaa ggattgtcat
     6721 aataggagac attttataat tgtcccctgt tattggaaga gtggaactag agatagtatt
     6781 tttgtttgtt gttgtttctt ttttgccaag gctgttatta gtgttttcag tagaagtcaa
     6841 aaggtctgaa atgatctcac tagacactat attgccatca cgatttctaa aaaccttact
     6901 aatgatactg tgtttaccag cactattaac ttttgtcgaa ctttcaaggt ttgaagttac
     6961 ataattttta acccctttta tagtagcatt aacagtttta cctgatgctt ctaaatttgt
     7021 gatatatgtg atgaaagctt cagtatctgt gttaatagct ccaattaatt tagctttttt
     7081 aaatgctgaa aagccatcac caccaccata aagaaagtcg ttgacaacaa cggtgtaagt
     7141 agttgttaag ttaatttctt caccattgtc tttataaacc ttaactatct tgaagggggt
     7201 atcagagttc ttaggatcat tatctgtata agtgtatgtt aaacctgaca tttgaagaaa
     7261 ataggtctgg ttttcatcgt attgctgatt taggacatcg taaatgtgtt gaccagtcat
     7321 ttgaatgact tgaaggatat taccaaatgg ttgtacagcc tgtgcagctc cccaggtgat
     7381 ggtccgatca tttttgacaa ctaggtcact tcgaatacca ccattattgg tcatagcaaa
     7441 gtcaacagtt ggaaaagttt tcttagcaat agtaagctga gctgttgtta ctaagttacc
     7501 gacaggagat tctttatcaa tattttctgt tttagaaata gttgaagaat tagttgcagt
     7561 tccgattttt cgttcagtaa ctgttttaac aatatcatta gcatgattta ttatagcttt
     7621 gatatctgaa ttttctgttt tgatacctgg tgctacagca ataacatttg ctgatggagt
     7681 tgtcataaaa tcattggtat cagtatctag cgtaccacgg acatctgcat aagcttttcc
     7741 ttgagagaga gcttgaacga tacgtgtttt accgatagtt ccattagtgt attgatgatt
     7801 atgtcctgca aaaataatat caatgctatg ttcgggatag atttggttca ctttttccat
     7861 aaccgtagcc atttcatgat caacaacacc atctttactt gttgcaggaa tatgagctaa
     7921 aaccacaata gcatgaacat gttgttcttg tagttcttta gcatatttgg caatggtttc
     7981 agctgcatct aaaaattgat agtgttcata gttttgcttt aaaacgagat ttggaatctc
     8041 tgtagtcaca acaccaatga agccaatctt aacgattttg tcattaatgg ctatgtcttt
     8101 tatagcataa ggtttccaac cataggggat atcattggtt tttttatcaa taacattagc
     8161 aatgacgatg gtttgatgcg aagcttcgtg ctcatattgt ttggtgatat cattaattgt
     8221 tgattcagga tcaggcgctt gacctgtcat gatacggtta aattcatcta gtccttcgtc
     8281 aaattcatga ttaccaagag tgccatattc aaatttcatt ttgttaaaga cttttacagt
     8341 aggctcatct tgtaaaagtg cagagttagc aggacttgct ccgaccatat ctccagcttg
     8401 aacacgtata cttgttccgt cttgatttgc ttgcttgaag tctatctcag cgtcatccat
     8461 ataagcacct aattgagcag ccgtcccagc atttggtatt ttaccacttg gtgtgtaagc
     8521 tgttccggta ttatcaagag cgccgtgaaa atcattgacg ccaaggaatt gcacatcaac
     8581 ttgatctgct tgaacgtctg taactaatag agtaaaacta gtcaggatac tcaatacact
     8641 actttttaaa ataaaatatt ttttcataaa atctcctaat aagttaacgt aatcgttata
     8701 gatcaagttt actatgtttt cgtttgtaat tcaatggtat ttttaaaaaa gaacgcacaa
     8761 aaaatatatg aaagcgattt aaaaacaaac gataaagatc gtttacagtt atatagttag
     8821 aaataaagat aaccgatcat tgtagagatt taagcatttg ttgagaaaat ttgttataat
     8881 aagcgtatga aagaaagact agaacattat tctaccagct ctatatatag cggatttgaa
     8941 gtgtacttgc cgttggtact acagacaata acagatgtga ttattgctga gaatatcaaa
     9001 agcaaaaaag aaacagggtt taaactatac gaacacttta catcccgtat taaatcggaa
     9061 gcttcgatga ttgaaaaatg tcaacgcaaa caacttcctc taacaagcaa atctgcatta
     9121 aaaatcatta aagatagtat cggtattcgc attatttgtg gttttattga tgatatttat
     9181 cggatggtag acttgctcaa aagcattcct ggtatgtcag ttaacactga aaaagattat
     9241 attttaaatg ccaagcctaa tggctataga tcatatcatc tcattttgga attagaaaca
     9301 cattttccag atattttagg ggaaaaaaaa ggatgttatt ttatagaagt gcagttgcga
     9361 accattgctc aagactcttg ggctagcttg gaacatcaaa tgaagtacaa acatcaagtt
     9421 gctaatgcag aaatgattac aagagagtta aagcgttgtg cagacgagtt ggcatcttgt
     9481 gatgttacca tgcaaaccat tcgtcaattg attcaagaaa cgacggagga agagtaacca
     9541 tgaaaatttt gttagctgaa gacgaatggc agatgtctaa tgttttgaca actgccatga
     9601 ctcatcaagg ttatgacgtt gatgtggttt ttaacggtca agaggcaatt gataaagcta
     9661 aagacaatgc atatgatatt atgattttag atatcatgat gccgattaag tctggcattg
     9721 aagctttaaa ggagattcgc gcttcaggaa attgctcaca tattattatg ctgacggcta
     9781 tggcagaaat taatgaccgt gttactggac tagatgcggg agctgatgat tatctgacta
     9841 aaccgttctc attaaaagaa cttttagcta gattaaggtc gatggaaaga cgagtagaaa
     9901 gttttactcc tcaggtatta caatttgctg gtgtcacatt aaatattaat gagcaagaat
     9961 tgagtgccgg taatgctata cgattagctt ctaaagaagg taaattaatg gcctttttaa
    10021 tgttaaatca aggaaagtac ttggatacta aaacacttta ccaacatgtt tggtcagacc
    10081 aagaagatta tgatacttct attgtttggg tttatatttc ttatcttcgt caaaaattat
    10141 tagctattca agcaaatgtc attattgcag gtgataaaga tactagctat tgtttattag
    10201 aaaaatagga ggtgacatgt ttaatcgtat ccgtattcgt tttatcatga ttgcttctat
    10261 tgccattttt attattttaa gttcaattgt tggtattatt aacacggcac gctgttacca
    10321 aagtcaacaa gaaattaacc gtattttaca tttaatttcc tcaaataaag gtaaattacc
    10381 tggcactacg gaaagctcta aacgacttgg aacgaaactg tctgaagata gcctaagcca
    10441 gtttcggtat tatagtgtca tttttaatgc taatggacat ctactttctt ctaatactgc
    10501 taatatttca gctttagata gagaagaagc ccaatatttt gctagacttt ttgctaagtc
    10561 tggggaagaa aaaggcagtt accgtcacca agatagtgtt tattcgtact tgattactca
    10621 gctgccaaat gaagaaaaac tggttgtaat tttagatact accttttatt ttcgtagtgt
    10681 aggagattta ttagctgttt cagtgatgtt ggcctttgga ggatttatct tttttgttgt
    10741 tttagtgagt cttttttccg gtatggtcat caaacctttt gtccaaaatt atgagaagca
    10801 gcgtcgcttt attactaatg caggccacga gttaaaaaca ccactagcca ttatctcagc
    10861 caataatgaa ttggtcgaat taatgactgg agaatcagaa tggaccaaga gtacaagtga
    10921 tcaggtgaag cgattgacag gcttgattaa tcaaatgatt actttggctc gtttggagga
    10981 acagccagat gtggtattac atatggtaga cttttcagct attgctcaag atgcagctga
    11041 agattttaag agtctggttt tgaaagatgg taaacgtttt gatttgacga ttcagccgaa
    11101 tattatgatt aaagcggagg aaaagtcatt atttgaactt gtgacaattt tagttgataa
    11161 tgccaataaa tattgtgatc ctaaaggctt ggtcaaggta tctttaacca ctattggaag
    11221 aagacgaaaa cgagcgaaat tagaagtttc taatacctat ttggaaggaa aatccattga
    11281 ttacagccgc ttttttgaac gcttttatcg tgaagacgaa tcccataata gcaaagaaaa
    11341 aggctatgga attggtttat ctatggcaga aagtatggtt aagttattta aaggaacgat
    11401 aactgtaaat tataaaaacg atgctattgt ttttacagtg gtgatttaac ttaaacattt
    11461 ttcagtacct ttagctagta ataatgtcta ggcaaaggta ataaatgatg cttaaccagc
    11521 ccatgaggct ggttttaacg tttaatttag tagaagagtg attaaagaag gtagtttgaa
    11581 ataagaaaat ttttgttatg atcctaaaaa gagtagatag tctttattgt taatttcagt
    11641 gaagaaatga ttttaaaaag atatatcatc gcattttcta atcatttagt gtaaaatagt
    11701 aaggtaaaag tttagaagaa ttagtaatga aaagataaaa aatgaatgaa aacattggat
    11761 atggtaaggc acacagtaag atcattttga taggtgagca tgctgttgtg tatggctacc
    11821 cagctattgc tttgccttta acagatattg aggtggtttg tcatattttt ccagctgata
    11881 agccattggt ctttgatttt tatgatactc tatcaacagc catttatgct gccttggatt
    11941 atttgcagcg actacaagaa ccaattgctt atgagattgt ttcacaagtg ccacaaaagc
    12001 gtggtatggg gtcttcggca gccgtttcta ttgcagctat tagagccgtt ttttcttatt
    12061 gtcaagaacc cctctcggat gatttgttgg aaattttagt caacaaggca gaaattattg
    12121 cccacaccaa tcctagtggt ttagatgcca agacatgcct tagtgaccat gccattaagt
    12181 ttatccgaaa cattggcttt gaaactatcg aaatcgcctt aaatggttat cttgtcattg
    12241 cagatacagg gattcacggt catacacgtg aggccgtcaa taaggtagca cagtttgagg
    12301 aaacgaattt gccctatctg gctaaactag gagctttgac acaagccctt gaaagagcga
    12361 ttaaccaaaa aaataaggtg gctatcggtc agctcatgac acaagcgcac tctgctttaa
    12421 aggccattgg cgttagcatc agtaaagctg accaacttgt ggaggctgcc ctaagagcag
    12481 gtgctttagg cgctaagatg acaggcggtg gcttgggtgg ctgtatgatt gccctagcag
    12541 ataccaaaga catggccgaa aaaatcagtc acagattaaa agaagaagga gccgtaaaca
    12601 cgtggatcca aatgttataa ccgtaacatc ctatgctaac attgctatta tcaaatattg
    12661 gggaaaagag aatcaagcta agatgattcc ttcgacctct agcatttctt tgactttgga
    12721 aaacatgttc accacaacca gcgtttcctt cttaccagat actgcaacca gtgatcagtt
    12781 ttacattaac ggtgtcttgc aaaatgacga agaacatacc aaaatttcta ctatcattga
    12841 ccaatttcgc caacctggtc aggcttttgt aaagatggaa actcaaaata atatgccaac
    12901 agctgcaggt ttatcatcaa gttccagtgg cttatcagcc ttggttaaag cctgtgatca
    12961 attatttgac actcagctag atcagaaagc tttagctcaa aaggctaagt tcgcctcagg
    13021 atcatcttct cgttcttttt ttggcccagt tgctgcttgg gacaaagata gtggtgctat
    13081 ttacaaggtt gagactgact tgaaaatggc catgattatg ctggtcttaa atgctgcgaa
    13141 aaagccaatt tctagccgag agggcatgaa gttatgccgc gatacctcaa ctacatttga
    13201 tgaatgggta gaacaatcgg caatcgatta ccaacatatg cttacctatc tcaaaactaa
    13261 taattttgag aaagttggtc agttaacaga agctaatgcc ttggccatgc atgccacgac
    13321 aaagacagcc aatcctccct tttcttacct aacaaaagag tcttaccagg ctatggaggc
    13381 tgtgaaagaa ctgcgtcaag aaggttttgc ttgttatttt accatggacg caggtccaaa
    13441 tgtgaaggtc ctatgtttag aaaaagactt ggctcaacta gcagaacgac ttggcaagaa
    13501 ctaccgtatt atcgtttcaa aaacaaagga tttaccagat gtctaattat tgtgtgcaaa
    13561 caggtgggaa actatacctc acaggcgaat atgctatctt aacaccagga caaaaagcct
    13621 taattcactt tattccactg atgatgacag cagaaattag cccagcagcc catattcaat
    13681 tagcttcaga tatgttttcc cataaagcgg gcatgacacc cgatgcctct tatgcactga
    13741 ttcaagcaac ggttaaaacc tttgctgatt atctaggaca gtcaattgac caactggagc
    13801 cattttccct aattataacg ggaaaaatgg agcgcgatgg caaaaaattt ggcattggtt
    13861 caagtggtag tgtcaccctc ttaaccttaa aggccttatc ggcctattat cagatcactt
    13921 taaccccaga gttactcttt aaactagcgg cttatacctt gcttaagcaa ggagataatg
    13981 ggtctatggg ggatattgcc tgtatcgctt accagacttt agtcgcctat acctcctttg
    14041 accgagaaca ggtcagtaac tggctgcaga ccatgccctt aaaaaaactt ctcgtcaaag
    14101 attggggtta ccatatccaa gtcattcaac cagccctgcc ttgtgacttt ttggttggct
    14161 ggactaaaat acctgctatt tcaaggcaga tgattcaaca agtgacagcg agcattaccc
    14221 cagctttctt aagaacaagt taccagctaa cgcaatcagc tatggtagct ttgcaagaag
    14281 gtcataagga agaactcaag aaaagtttag caggagcaag tcatctccta aaagagcttc
    14341 attcagctat ctaccatcct aagctagtaa ccttggtagc tgcttgtcag aagcaagatg
    14401 ctgttgctaa atcttcaggt tctggtggtg gagattgtgg catcgcgctt gcctttaatc
    14461 agaatgccag agataccctt atttccaaat ggcaagaagc tgatatcgca ttactttatc
    14521 aagaaaggtg ggaagagaat gaccaaccgt aaagatgatc acatcaaata tgccctcaag
    14581 taccaatcgc cttataatgc ttttgatgac atagaactca tacatcattc cttacctagc
    14641 tatgatttgt ctgatattga tctcagtact cattttgccg ggcaagactt cgactttccc
    14701 ttttacatca atgccatgac aggaggaagt caaaaaggca aagctgtcaa tgaaaaattg
    14761 gccaaagtag cagcagcaac agggattgtc atggtgacag ggtcttatag cgctgcttta
    14821 aaaaatccta acgacgattc ctatcgttta catgaggtgg cagataactt gaaactagcc
    14881 acgaatattg gtctagataa acctgtggcg ctaggacaac aaacggttca agaaatgcag
    14941 cccctctttt tacaggttca tgtgaatgtg atgcaagagt tgctgatgcc agagggtgag
    15001 cgcgtctttc atacctggaa aaaacacctc gctgaatacg ctagtcaaat accagttcct
    15061 gtcattctca aagaagttgg ttttggcatg gatgtcaata gtatcaagct agcacatgac
    15121 ctaggcattc aaacctttga tatttcagga agaggaggga cctcatttgc ctatattgaa
    15181 aatcaaagag gaggagaccg ctcttactta aacgattggg gacaaaccac cgttcagtgc
    15241 ttgctaaatg cacaaggact gatggaccaa gtggaaatct tagcttcggg tggtgtcaga
    15301 caccccttgg acatgattaa atgtttcgtc ctaggagcac gtgcagtggg actctcacgc
    15361 accgttttag aattggtcga aaaataccca accgagcgtg tgattgctat cgttaacggc
    15421 tggaaagaag aattaaaaat cattatgtgt gctcttgact gtaaaactat taaagaatta
    15481 aagggagtcg actacttact atatggacgc ttgcagcagg ttaattaggc ttcatcacct
    15541 ctacaatagt gccacctact acaaagatga tagagctttt ttagaacata atataaaata
    15601 tttataaata cctcatcaaa ctcagaatag agtagatgag taaatagagt atgttatgga
    15661 gactatttct aagacttgta tgtatagaaa caaagattaa acacccatgt aaaccaattg
    15721 tttacatggg tgtttgcctt atattagttg tttaaatcta agactgtctt agctgggata
    15781 agtaaaaatg tgctttttct agatttattg gtttatcagc tagtaaagca ttaactaaag
    15841 gagcaatttc gtcttgggtt gcccccgcca aaagagctaa tgatttcgca tgtagtttca
    15901 tgtgaccagt ttggatgcca gatgaggtca gagcttttag tgcagcaaag ttttgagcta
    15961 accccacaga tgcaatcaat tgggctaatg ttttggcatc aggttgatta agaagatcat
    16021 gtgcgataga aacagtaggg ttaagcccga tagatccccc cttactagca ataggcatag
    16081 ggagggtcat ttggccaagc agaacttgtt tatcttggtc aaaatgccac tgactaagtc
    16141 cttgatagct accatttctt gaggcataag catgggcccc tgcttcaata gcacgccagt
    16201 catttcctgt ggctattacc actgcatcaa tgccattaaa aataccttta ttatgagtag
    16261 tagcccggta aggatctact tgagctagtc ggctggctag ctctattttt tgagctaact
    16321 gtagggattt tgttttatcg tgatctaaaa ggcgcacggg aatctcacaa ctagtagtaa
    16381 caagcgactc tgttgcgtaa ttagataaaa tcgccattag acaatgcccc ttagacagtc
    16441 ttgttaaatc aggaacaaga gcttccatca tagtattgac catatttgct cccatagctt
    16501 cttgggtatc aacagtcaga tagaaaatca aaaactctcc tttttcttcc aaatgaatgg
    16561 ttctagcgcc tccacctctt ttaacaatgg aaggataagc tttatttgcc aatgcaatta
    16621 gcttttttgt tttatgaagg atggcggctt tagcgttatc tatttggtcg atatcgtaaa
    16681 gaacaatttg accaatcatt tgtcggttta gggtttgagc tttaaaacca cctgagcgtt
    16741 tgattagttt tgctgcgaaa gatgctgcag caacaacaga aggttcttcc gtgacaaaag
    16801 gcatctgata agttgaaccg ttgacaagaa aatcaggagc tatgctaaag ggcaaagcca
    16861 atcgtcctaa gacattttca gtcatttgat tagctgtttg gataggcaaa agaacgtctg
    16921 ttttgagttg atttaagttt tcagcattaa gtagtttaaa tttttcgata agttggaggc
    16981 gttcttcgaa tgttttcttt gaaaaaccgc tccagttaag attagttttt gtcattggta
    17041 tgatagatcc ttcgatgctc cttgatggct gttagagcaa attttccagt taagtaaatg
    17101 tcaaaattgg cattaccatt atcatctaat tggacttgtt cgtagaaaag gtcttcgtat
    17161 ttagaaacgg aaagacgtgt tcgttgatcc agtaaagctt gtcgattagt cattaacatt
    17221 ttatcgtacc cagcaactaa ctgaccggaa taaaattcac ttacggcacc ggaaccatag
    17281 ctaaaaaaac caattttatc tccagattgt aatactttac tattttcaag taaagataac
    17341 aatcctagat aaagtgagcc agtataaata tttccaattt gtttgctata agtaatagaa
    17401 gcttgaaagg cttctattag tttttccctg tgttcaggag gtactgtgtt atccataatg
    17461 ttatttagac cttttagggc taacttggga aatggaatat ggaaacaaac tgcagcaaaa
    17521 tcagataact gaaggttttc tcttttctga taagcttgcc acgtggtttc taagcaattg
    17581 agatattgct tggtagagta aataccatca acataaggtg tgaaactata gttaggcctc
    17641 caaaagtcca taatatccct agtttgagcc acattatctt catttagagc aagaatacga
    17701 gggttagcag ttaccaaaag agcaatacta ccagagcctt gagttgattc gccaggagat
    17761 cctacaccgt atctagcaat atcactagca ataacaagga cacgagaatc tggcttagaa
    17821 gccacatgca gtttagcata gtctagagca gcagttgcgc tatagcaggc ttgttttact
    17881 tcaaaggaac gtgcaaaagg ctggatacca actaagtgat gtacatagat agctgatgcc
    17941 tttgactgat cagtactcga ttcagttgcc aaaataacca tatcaatctt agatcgatct
    18001 tcgtcggtta agatttgatc tgctgcagaa gcagctaaag taataatgtc ttcagtgatt
    18061 ggtgccacac taaaagactc aatgagtagc ccttgactaa attttgctgg atccacttgg
    18121 cgtgcaagcg ctaaatcttc caatttcaag acatattgac tggttgcaaa accaatctta
    18181 tcaattccaa ttgtcatatt tgtccttctt ttagttattt ttatagtctt acccattcta
    18241 tcacatttta ctgcaaaatt agactgataa agttatttgt tt
//
LOCUS       DATXQK010000031        17740 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.031, whole genome shotgun sequence.
ACCESSION   DATXQK010000031 DATXQK010000000
VERSION     DATXQK010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17740
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.031"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(113..490)
                     /locus_tag="VC394_001183"
     CDS             complement(113..490)
                     /locus_tag="VC394_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEP5994654.1"
                     /translation="MLIYPATFTQDTDYIMVTFPDVPEAITQGEDFQEAYEMAVEVLG
                     FALEDYTDYPKASAVSDLKEQYPDSDIALIGIDMVAYMKKYHSKKVRKNVTIPEWLND
                     AAEDKNLNFSQVLTEALELKLQA"
     gene            complement(542..727)
                     /locus_tag="VC394_001184"
     CDS             complement(542..727)
                     /locus_tag="VC394_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEP5994655.1"
                     /translation="MPMTPKQMIKLLKKNGFYEVSQNGSHKKFRDNNGHQTIVPMHNK
                     DLGKGLEDTILKQAGLK"
     gene            790..1257
                     /locus_tag="VC394_001185"
     CDS             790..1257
                     /locus_tag="VC394_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEP5994656.1"
                     /translation="MRIKYTKGGDGKLSKLTLKQKRFADEYIISGNAMESAIKAGYSH
                     NYSKAQSHKLLENVGIKEYLDERFEKMDSKTIADQKEVLQYLSSVMRGEQQEKTLISI
                     GELGQEIVDIDVGAKDRLKAAELLGKRYRLFTDKIEAEVQGTVVFVNEDDIPD"
     gene            1235..2542
                     /locus_tag="VC394_001186"
     CDS             1235..2542
                     /locus_tag="VC394_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEP5994657.1"
                     /translation="MKTTYQTKPKLEIKVDLPKTIGIGYGAFWRSKNFYRVVKGSRGS
                     KKSKTTALNFIVRLLKYPWANLLVIRRYSNTNKQSTYTDFKWACNQLKVTHLFKFNES
                     LPEITVKATGQKILFRGLDDELKITSITVDVGLLCWAWFEEAYQIETEDKFSTVVESI
                     RGSLDAPDFFKQITVTFNPWSERHWLKRVFFDEETKRADTFSGTTTFRVNEWLDDVDK
                     RRYEDLYKTNPRRARIVCDGEWGVAEGLVFDNFEVVDFDVEKTIQRVKETSAGMDFGF
                     TQDPTTLICVAVDLVNKELWLYNEHYQKAMLTDHIVKMIRDKNMHKSYIAADSAEKRL
                     IAEIKSKGVSGIVASLKGKGSIMQGVQFMQGFKIYIHPSCEHTIEEFNTYTFKQDKEG
                     NWLNEPIDKNNHVIDAIRYALEKYHIRSNESNQFEVLRAGFGY"
     gene            2554..4056
                     /locus_tag="VC394_001187"
     CDS             2554..4056
                     /locus_tag="VC394_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEP5994658.1"
                     /translation="MYTESFRDSTGKTKTLEFRFHREARMRYQAESLESLLTEKYKLL
                     REMIEHHDKVQKPRIQELLDYAEGNNHTISEIGRRKDDDMADVRAVHNYGKYISTLKQ
                     GYLVGNPIRVEYIDGTEQQQDLLKDLSVKNNFHQLNRRLVKDLSKVGRAFELIYRSMD
                     DKTEVVRLDPREVFVIYQNNLEQSSLAGVRYYNKNQLDGTTKIVELYTDNKILKFEYD
                     GDLTLIREASSHAFGSVPITEYLNTDDGMGDYETELSLIDLYDAAQSDTANYMQDLSD
                     AILAIFGRVSFPHDVQTAEQRIEFVKVMRKARLLNLEPPVDQDGREGSVDAKYLYKQY
                     DVQGTEAYKNRIVSDIHKFTNTPDMTDSKFAGQQSGEALKWKVFGLDQERVDMQALFE
                     QSLKRRYKLIARVSQLLKEIDDFDISKLKITFTPNLPKSLQEKIEAFKALGGELSQET
                     AMAITDIVEDAKKEISLINSESKSRSQLAQKLEETSRLTDRELAHDHQKE"
     gene            4037..5599
                     /locus_tag="VC394_001188"
     CDS             4037..5599
                     /locus_tag="VC394_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HEP5994659.1"
                     /translation="MTTRKSKYWRDRIKKEMDAKEADDISLEQSMKQLHDYHFRNIEK
                     EIESFYQRYADKEKIDLSEARKRASELDISAYQKKAKELIAKAEKLRKEGRTVTKADF
                     THQENADMAIYNLAMKTNALELLRLNIDLEMQELANGEHKLTKKFLDEGYRKEVEFQA
                     GLLGLSVASQANVKNLADAVINANFKGAKWSDNIWDRQDKLRSIISQSVQSAILKGKN
                     GLTIARDIRREFDVSASYAKRLAITEHARVQMEVGRLSMAENGFAMFDILPEPKACDV
                     CKDIAKHGPYHLDKWRIGENSPPFHPYCRCAVVGLDKKQEEKVNDKSEKFENITPNLM
                     PLFSKFASKITDLQRKIVYSADLADTGYIRTPHAFDINNTLRNKGYNYLNVDDKLITD
                     TLDSVISINSTPKNIKVYRFDDFELLGSINEQNNNIFDSGNFMDKLNQGGLSYTNDGY
                     TSASYDVKKNVMGYRPIKTEIKVPKGSHVYLTDNEEESEIILPRGTKYDIINAKINEY
                     EEIEITMEIRKE"
     gene            5603..5788
                     /locus_tag="VC394_001189"
     CDS             5603..5788
                     /locus_tag="VC394_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994660.1"
                     /translation="MDFSDFLNKKQKEWDESHPIPDFSAMSDEELLYQPMSEALVSER
                     FAKELSKEVRKRNLLSK"
     gene            5858..6172
                     /locus_tag="VC394_001190"
     CDS             5858..6172
                     /locus_tag="VC394_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994661.1"
                     /translation="MNRESKPGMESVKIGGLTYSVEKASDLQGKDGNWGLIHYKTQQI
                     KLDDSLTEQLEDQTLIHEIVHGILVEAGYTNHEEDQANRIGLILYQVLQDNDFSWLYK
                     GE"
     gene            6175..6441
                     /locus_tag="VC394_001191"
     CDS             6175..6441
                     /locus_tag="VC394_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994662.1"
                     /translation="MNKRIKKKRKLETAVVMLVAENAIQAKALRNQNKQIAELRAIIQ
                     QNVQAINREFVTAKNTILDNQLAIKLIGDDVGHIKQNYKRKWRK"
     gene            6585..7118
                     /locus_tag="VC394_001192"
     CDS             6585..7118
                     /locus_tag="VC394_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEP5994663.1"
                     /translation="MAEETQVVEMVEEQVVPEAKQPQDEKKYTDADVDAIIDKKFAKW
                     KSEQEAEKSEAKKMAKMNEKEKADYEKQKLLDELQELKNDKTRNELTAVARQMFAESE
                     INVNDDVLGLVVTLDAEQTKANVTTLANAFAKVIADDRKALVRQTTPSTGGGLRKQTN
                     YGANLASKAAQQSTKLF"
     gene            7128..7508
                     /locus_tag="VC394_001193"
     CDS             7128..7508
                     /locus_tag="VC394_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HEP5994664.1"
                     /translation="MNKRKVTTSKEILHNLDYEAISVTLDSKKIGKEVVPAGTVLAGV
                     SGSVFEDRKQKVKAVDNSAVESEDNICGILLTDVDLTNGDAVGSCIYRGTINADKLAD
                     SSIAGKYEVLEEELPHIVFIKGGK"
     gene            7511..8593
                     /locus_tag="VC394_001194"
     CDS             7511..8593
                     /locus_tag="VC394_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HEP5994665.1"
                     /translation="MALIHEIITSENIKGFYNAKNENVGNTLGENAFPPKQQLGLKLS
                     FIKGAAGKPVTLKAAAFDTKVPLRDRMTVELLDEEMPFFKEAMVVKEADRQQLSLLAQ
                     TKNQELIDTALAAIYNDKVTLIAGAKARLEAMRMEVLSKGKIHVASNGVMKDFDYGLD
                     SSQTTKPDTKWEQVDTATPLKDIEKAIEVMAERGFVPEAIIMNSKTLSLIKNASNTLD
                     VVKPMAPDGAAVTKGDLETYIADELGLKILLKDGMFVGDDGKAKKYFPDGFATLVPNG
                     NLGYTVFGTTPEQSDLLGGEATDAEVSIVETGIAITTTKTTDPVNVQTKVSMIALPSF
                     ERLEEVQIINATESEEETKKENSFEM"
     gene            8603..8845
                     /locus_tag="VC394_001195"
     CDS             8603..8845
                     /locus_tag="VC394_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994666.1"
                     /translation="MPRVIRAFKDKVTKVVYEVGDIYSGDRVEFLTEGGVLEPSVDFD
                     KLKVSEIKSKLDELSIEYDAKLKKSELLKLLKQTIG"
     gene            8859..9212
                     /locus_tag="VC394_001196"
     CDS             8859..9212
                     /locus_tag="VC394_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HEP5994667.1"
                     /translation="MDAVNTSSVISNVKLDLGILDSQQDDLLNMLLKRVTDHFKAKYG
                     AVEIDSAFSFVLEDCLIARFNRRGAERAKSESVEGHTTTYYDFLDEFEPYDAMIMAKL
                     NLIKDKSRKGGLYFL"
     gene            9209..9517
                     /locus_tag="VC394_001197"
     CDS             9209..9517
                     /locus_tag="VC394_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994668.1"
                     /translation="MRYADRVTFVKTTDEQYNPDLGEYTHTEVISITKPCFVMDMGME
                     KSVQIFGDYQKDRKVIYLKQPYTKAFDYCEYEGKRYKAQANKLGAIVFYLEGDDSIGG
                     "
     gene            9498..9863
                     /locus_tag="VC394_001198"
     CDS             9498..9863
                     /locus_tag="VC394_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEP5994669.1"
                     /translation="MILLVADISLKVVGTAGLKKKLELIVKKDAVKKIVRDNGTQLQR
                     KMINKAVFTKGYSTGATRRSITMQIGDGGLSVKVKPGTHYAGYLERGTRLMSKQPFVL
                     PALKEQKVKFRKDLEALVK"
     gene            9860..10249
                     /locus_tag="VC394_001199"
     CDS             9860..10249
                     /locus_tag="VC394_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HEP5994670.1"
                     /translation="MIKTRDQSIFDEMFKRIQSLGFKVYDYKPMTEVPYPFVEMESTD
                     AEYIPNKDDIKGSVELMLSVWGVQKKRKQVSDMASAIFSQAMSVARTDGFCWSFNIRQ
                     SSIQMLDDTTTVTPLKRAIVTLRFNLR"
     gene            10304..10927
                     /locus_tag="VC394_001200"
     CDS             10304..10927
                     /locus_tag="VC394_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HEP5994671.1"
                     /translation="MSKAQEQTKQLEAKQGIHSILLFRLLKEASSEVATKLAFQTEHE
                     VGKSRDVDGQKTKDGIIQSVGALEYDFKATSILAKGDELAAKLEKAMEDGELVEIWDI
                     DSEEISKNGDSDNKLAKVWGINKNGTNGGNGKCLATYYQGYISSFSAKKNAEENIVIE
                     MEFAINGVGKKGFATLTDAQKAAVQYAFKDTTKSNSEETKKENSFEM"
     gene            10981..11334
                     /locus_tag="VC394_001201"
     CDS             10981..11334
                     /locus_tag="VC394_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HEP5994672.1"
                     /translation="MQLEIKGKTHNVKFGTRFVAEMDKNHIAERQGFKFGAGLQSSVP
                     FLIDHSVVTLAEVIYTGTITEPPRPSLNDIYDYIDEVEDIEKLFDDVLDELRQSNASK
                     LFMAQVEKDLAEAKA"
     gene            11409..11705
                     /locus_tag="VC394_001202"
     CDS             11409..11705
                     /locus_tag="VC394_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994673.1"
                     /translation="MTDINEIGRLTLYEYDLLMTGKALAAVDESHKAHKQAWINHQVT
                     ATKLVGGKKNKKEVPVYKKFKDFFDYEEEIRKITQEIDEGYDKKGMDLLLKANL"
     gene            11720..15355
                     /locus_tag="VC394_001203"
     CDS             11720..15355
                     /locus_tag="VC394_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HEP5994674.1"
                     /translation="MGESYSVEAVLTAVDKTFGKTLQSAIRSIEGLEKRSTGFSSVSQ
                     KASSMFKSMLGANLAGQAISAMTRTVSSGLGSMLGEMNSSAKAWKTFDANLADIGFGK
                     KQILAAKTAMQDYATKTIYSASDMASTYAQLAAVGVKDTGKLVKAFGGLAASAENPKQ
                     AMKSISQQMTQAVGRPTVAWQDFRIMLEQAPAGMAKVAKSMGKNLDELVADIQAGRVK
                     TSDFLEAVKKAGNDKSFQKMATEFKTVDQAIDGMREGLSNKLQPAFEKVNQFGIRAIE
                     AIGKQLDKVDFSKFASNLGKFLEGINIDKIVSNISSAISSVTSKVKEFWDGFKQTGAI
                     SAFSGALQSVWEALKNVASAMSGGNWKTFGATVGGTVKHVSNFAKAVSDVLGKMDPGR
                     LRSWIATFAAVAGGFKLFEKLTGQSVIGSFLDKIGSKFGLFGNKAKEGTDKASNGARR
                     SGGIISQIFSGLGNIVKSAGTAISTAAKGIGAGIKTALSGIPPIISSLGTAISTVAQG
                     IGTGLAIAFKGLGSAIAMVPPTTWLALGAAILMVGAAFALAGTQADGISQILRTVGDV
                     VVQILQQVTDSLATLLPIIANAIGSMLPIVAGAISQIVGAVAGGLSQLVIAVSTGASL
                     VIGAFTGLLGGISGVINSISAVIQSLTGVITAVFNGIATVISSVGSAIKDVLTGLGTA
                     FEGFGNGVKSALEGVGAVIESFGSAVRNVLDGVANILDSMGTAALNAGRGVKEMAKGI
                     KMLVDLSLGDLVATLAAVASGLGKMASSAGEMTTLGSAMSKVANGMTRLATSATIAIT
                     GLTVFATTMATIKTAVATLPPVLTMAASGFTTFTTQAVAAVTGLAAINAPITMFKAQL
                     MTITPALAQAGAGFAAFVAQSSTFSTGLASAGPTIAAFNANLMSLSATTGALVASIAG
                     LSAVLSVVSAGFSQIGASATATVGQIQAFASSTTVVSSAFASMQSMIQSAMAAIVSSI
                     ITSFNQAASQMQSILSRMLSQARTFGSQLEQQMRQSGQRSGQNLARGLSSQQSAVINA
                     ISSMVNAAVSRANAGAGPMRQAGAYIGQGLAQGMYSALGAVTAAANALVAQAERAARA
                     KAMIHSPSRLFAKRVGQYIPQGVAMGIDKNADVVDDSVGGLFDSINSFDFNIADRLAS
                     IGAKFQGVIKSESSQSLSQQQEFVHTAQPAYINFSLGGNEYEAFVSDITNQQAKIEKI
                     RLKRSSW"
     gene            15387..16166
                     /locus_tag="VC394_001204"
     CDS             15387..16166
                     /locus_tag="VC394_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HEP5994675.1"
                     /translation="MYEFNDTIRGTPKVTFNLKTTIGEKVLEEELNNDLGTFRTLTVS
                     GRDVVDLEHQTTSVLGRNGEYFHNATVEVRKLEIKAKITGKDNQSMRLQYEKLNRLIV
                     SHNQVFLSFSDEPDRNYLGIFKSKDVPEEVSNEQIIGLTFICYNPFKMSDVKTKKGTS
                     IQNGGLFQTKPIITLNLSSPTKEIKLLHVESQKYIRLTGTYTTDEIKIDMATGKITQN
                     GRNILGDLDMINSRYFELLPGNNTLQCANAAITAEFREVYL"
     gene            16163..>17740
                     /locus_tag="VC394_001205"
     CDS             16163..>17740
                     /locus_tag="VC394_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEP5994676.1"
                     /translation="MIYLFDKLERLVATVGTDDLLSWHFKVKNNDWDQASFEVPVDYD
                     VEPFVYFGFFNYDPNQKEDVFKLFKVIDYNLEDSKFYKGLDKAESDLDTIAIIKDKRF
                     RQSSADACIDGALEGTGYQVGKVEGITNVRTLSYYYISPRAALIKIVEAFNCEFNVRY
                     TFINNKITSRYIDLKKRFGKPTGKQFEHGNNLLKVVYEESTDDIVTCLIGRGKGEEIQ
                     HEEAEPKDVEGHLPQEERRQGYGRRIEFTDVVWSVEKGDPIDKPAGQNFVALDSAREE
                     YGLSQNGELKHRWGVFVNEEIEDKTELLKATWEELQRLSIPIRIYKAEILDIGPETWK
                     GDSVAIIYDEVKIAFETRVDEIDIDKLNFNRSVVTLGDYSVVQNRESRSRKEAVQNMI
                     DESLETITDLGMTFQEFLQGIEKRIETGKKEMEDNWRKVNLEFDNFKKKVEQEGLQFN
                     TLKEQIKEVDERTDKELEEFRATLKNLALPEEAIKKITEAIKVDDIPSLKQNFDDLKN
                     KVSETSETARLNAEIIGT"
BASE COUNT         5980 a         3074 c         3953 g         4733 t
ORIGIN      
        1 gttatggtcc acgacatggt tacttattag ttacaaacgt tgaaattaca atgtttcgat
       61 aggtcaacgt cgtggttttt tattatgtaa aaaagaacca caacagtggc tcttatgctt
      121 gtaattttaa ttcaagcgct tcagtaagaa cttgagaaaa gttgaggttt ttatcttctg
      181 ctgcgtcgtt taaccattca gggatagtca cgtttttgcg gacttttttg gagtgatatt
      241 ttttcatata ggcaaccata tcgatgccaa ttaaagcaat gtcggaatca ggatactgtt
      301 cttttaaatc agaaacggcg ctagcctttg gatagtcagt atagtcttca agggcaaaac
      361 ctaagacttc gacagccatt tcataagcct cttgaaagtc ttcaccttga gtaattgctt
      421 caggtacatc tggaaatgta accatgatat agtctgtgtc ttgtgtaaat gtagctggat
      481 aaattaacat aatgatcctc ctttgattac tgtgagataa gcacaccatc tggcaagcgg
      541 attattttaa acccgcctgc tttagaatgg tatcttcaag accttttccg aggtctttat
      601 tgtgcattgg aacaatggtt tgatgtccgt tattgtctcg gaattttttg tgacttccgt
      661 tttgactgac ttcgtagaac ccattctttt ttagcagttt tatcatctgc tttggggtca
      721 ttggcatatt gcttacctca ctttctatac ttatattata cacataaaca atgtagatgt
      781 caatgcaaaa tacgcataaa atacacaaaa ggcggtgatg gaaaattgag taaattaaca
      841 ctaaaacaga agcggtttgc agatgaatac atcatctcag ggaatgctat ggagtcagct
      901 ataaaggctg ggtatagcca taattattcc aaagcacagt cgcacaagct attggaaaat
      961 gttggaatta aagagtatct tgatgaacga tttgaaaaaa tggattccaa gacaatagca
     1021 gaccaaaaag aggtattgca atatctttct tcggtcatga gaggtgagca gcaagaaaaa
     1081 acactcatca gcattggaga gcttgggcaa gaaatagttg atattgatgt aggagcaaaa
     1141 gacaggttga aagcagctga attgcttggt aagcggtaca gattgtttac cgataagata
     1201 gaggctgagg tgcaaggaac ggtggtgttt gtcaatgaag acgacatacc agactaagcc
     1261 aaagctagaa atcaaagttg atttacctaa aactatcggt ataggttatg gcgctttttg
     1321 gcgctctaaa aatttttatc gagtagttaa aggcagccgt ggatctaaaa aatctaaaac
     1381 gactgcttta aattttatcg tcagactgct gaagtaccct tgggctaact tattggtcat
     1441 ccgtagatac tcaaacacta acaaacaatc tacttatacc gattttaaat gggcgtgtaa
     1501 tcaattaaag gttacacacc tttttaagtt taatgagagc ttgccagaga taaccgtcaa
     1561 ggcaacagga caaaaaatct tattccgtgg tcttgatgat gaactcaaga taacatctat
     1621 tactgttgat gtcggtttgc tctgttgggc atggttcgag gaggcttatc aaattgagac
     1681 cgaagataag ttttcaacag ttgtcgaatc aatccgcggt agtttagatg ctcctgattt
     1741 ttttaaacaa ataacagtca cgttcaaccc gtggtcagaa agacattggc ttaagcgtgt
     1801 cttttttgac gaagaaacta aacgggccga tacattttct gggactacaa catttagagt
     1861 aaacgaatgg cttgatgatg tcgataaaag acgctacgaa gatttgtaca agactaatcc
     1921 aaggcgggct agaatcgtct gtgatggtga atggggcgtt gctgaaggtc ttgtttttga
     1981 taactttgaa gtcgtagatt ttgatgttga aaaaacaatt caacgtgtca aagagacttc
     2041 agctggtatg gactttggtt ttactcaaga ccctacaact cttatatgtg ttgcagttga
     2101 cctcgtaaac aaagaattat ggctttacaa tgaacattat caaaaagcta tgttgacaga
     2161 tcatattgtc aaaatgataa gagataaaaa tatgcataaa tcttatatcg cagctgatag
     2221 cgctgaaaaa cgtctaatag cagagataaa aagcaaaggt gtatctggta ttgttgccag
     2281 tcttaagggt aaagggtcta ttatgcaagg agttcagttt atgcaaggtt ttaaaatata
     2341 catccaccct tcttgtgaac acacaataga agagtttaat acttacactt ttaagcaaga
     2401 caaagaaggt aattggttaa acgaaccgat agataagaat aaccacgtta ttgatgcgat
     2461 tagatatgcg cttgaaaaat accatatcag aagcaacgag tcaaatcagt ttgaagttct
     2521 tagggctggt tttggttact agaaaggaaa ataatgtaca cagaatcatt tagagatagt
     2581 acgggaaaga ctaaaacatt agagtttagg ttccaccgtg aagctcgcat gaggtatcaa
     2641 gcggaaagtc tagaaagctt gttaaccgaa aaatataagc tactccgtga aatgattgaa
     2701 caccacgata aagtccaaaa accacgcata caagagcttc tagattatgc agagggaaat
     2761 aaccacacca tcagcgaaat aggccgtagg aaagacgatg acatggctga tgttcgtgct
     2821 gtgcataact atggtaagta tatttcaacg ctcaaacagg gctatttggt gggtaatcct
     2881 attcgtgtag agtatattga tggtaccgag cagcagcaag acctattaaa ggacctatct
     2941 gttaaaaaca atttccacca gctgaaccgc agattagtaa aagacctatc caaggttggt
     3001 cgagcgtttg aattgattta tcgcagcatg gatgacaaga cagaggtcgt tagactagat
     3061 ccacgggaag tatttgttat ctatcaaaat aacctagagc aatcaagctt agctggtgtg
     3121 cggtactata acaaaaatca attagatggt actacaaaaa ttgtcgagct ttacaccgat
     3181 aataaaatcc tgaagtttga atatgatggt gatttaacac tgattaggga ggcttcgtct
     3241 catgcgtttg gttcggtacc aatcacggag tatctcaaca cagatgacgg catgggcgac
     3301 tacgagacag agttgtcttt aatcgacttg tatgatgcag ctcagtctga tacagctaac
     3361 tacatgcaag atttgtcaga cgcaattcta gccattttcg gacgtgtgtc atttccgcat
     3421 gatgttcaga cagccgaaca gcgtatcgaa tttgtgaaag tgatgcgtaa agctagattg
     3481 cttaacctcg agcctcctgt cgaccaagac gggcgtgagg gatctgtaga tgccaaatat
     3541 ctatataaac aatatgacgt acaaggaacc gaagcctata aaaatcgtat tgtgtccgac
     3601 atccataaat ttaccaatac gccagacatg acagacagta agtttgccgg tcaacaatcc
     3661 ggagaggcgt tgaagtggaa agtgtttggt cttgatcagg agcgtgtcga catgcaagct
     3721 ttatttgagc aatctcttaa acgtaggtac aaactaatcg ctcgtgtaag ccaactgctt
     3781 aaagagattg atgactttga catcagcaag cttaaaatca catttacgcc aaacctacct
     3841 aagtcgctac aagaaaagat tgaagccttt aaagcattgg gtggagagtt gtcgcaagag
     3901 acagctatgg ctattacaga catcgtggaa gatgctaaga aagaaattag ccttatcaat
     3961 agcgagtcga aatcacgtag tcaactagcg cagaagttag aagaaaccag tagattgact
     4021 gatagggagt tagctcatga ccaccagaaa gagtaaatac tggcgtgacc gtatcaagaa
     4081 agaaatggat gctaaagagg cagacgatat ctctcttgag caatccatga agcaattgca
     4141 cgattatcat ttcaggaata tcgaaaaaga aattgagtcg ttttatcaac gttatgctga
     4201 caaagagaaa atagaccttt cagaagcccg taagagagct tctgagcttg atatttctgc
     4261 ttaccagaag aaagctaagg aacttattgc aaaggctgag aagctacgaa aagagggtag
     4321 aacggtaaca aaagccgact tcactcatca agaaaatgca gacatggcta tttacaactt
     4381 agccatgaaa accaacgctt tagaattgtt gcgcttgaat atcgacttgg agatgcaaga
     4441 acttgccaac ggcgaacaca agctaaccaa gaaatttctt gacgaaggtt accgtaaaga
     4501 ggttgagttc caagctgggc tattaggatt atcagttgct agccaagcga atgtgaaaaa
     4561 cttagctgat gccgttatta atgctaattt caagggagca aaatggtcag ataacatttg
     4621 ggacagacaa gataagttac gtagcatcat atctcaaagc gtccaaagtg ctatcctaaa
     4681 aggtaaaaat ggcttaacta ttgcaagaga tattagacga gagtttgatg tgtcagcatc
     4741 ttacgcaaag cgactagcga taacggagca tgcaagggtt cagatggaag ttggtagatt
     4801 atccatggcg gagaatggct ttgctatgtt tgatatattg cctgagccta aagcatgtga
     4861 tgtttgcaag gatatagcta agcatggtcc atatcacctt gacaagtgga gaatagggga
     4921 aaactctccg ccgtttcatc cgtattgtcg ttgtgcagtt gtaggattgg ataagaaaca
     4981 agaagaaaaa gtgaatgata aatctgaaaa atttgaaaac ataacaccga atctcatgcc
     5041 attatttagt aagtttgcaa gtaaaataac agatttgcaa cgtaaaatag tgtactctgc
     5101 tgatttagca gataccgggt acattagaac accgcatgcg tttgatataa acaatacctt
     5161 gcgaaataaa ggctacaatt atctaaacgt tgatgataaa ctaattaccg acacattgga
     5221 tagtgtcatc tcgataaatt caactcccaa aaatataaaa gtataccgat ttgatgattt
     5281 tgagttatta ggttcaatca acgaacaaaa caataatatt tttgattcag gtaattttat
     5341 ggataaatta aaccagggag gattatcata caccaacgat ggatatactt ctgcaagtta
     5401 tgatgttaaa aaaaacgtga tgggatatcg tcctattaag actgaaataa aagtacctaa
     5461 aggaagccac gtttatctta cagataatga ggaagaaagt gaaattatac taccacgagg
     5521 gacaaagtat gatataatta atgcgaagat aaatgaatat gaagaaatag aaatcactat
     5581 ggaaataaga aaggagtaag cgatggattt ttctgatttt ttgaataaaa aacaaaaaga
     5641 gtgggatgaa tctcatccaa ttcctgactt tagtgcaatg agtgatgaag aattgcttta
     5701 tcagccaatg agtgaagctt tagtgtctga aagatttgct aaagagttat ctaaagaggt
     5761 tagaaagcgt aatttattat caaaataaaa acatattatt atagacggaa gactgtggtc
     5821 gcccgtcttt ttttgtaccc aaaaacagga ggaaaacatg aatcgtgaaa gtaaaccagg
     5881 tatggaaagc gtaaaaatcg gagggttgac atactctgtt gaaaaggcat cggacctgca
     5941 aggcaaagat ggcaattggg gtcttataca ctacaaaaca caacaaatta aactagatga
     6001 ctcattaacc gagcagctcg aagatcaaac acttatccac gaaattgttc atggaatttt
     6061 agttgaggca ggctatacta atcacgaaga ggaccaagct aatcgcattg gtctgatttt
     6121 gtatcaagta ttgcaggata atgattttag ctggttgtac aaaggggaat aaatatgaat
     6181 aaacgcatca agaaaaaacg taagttagaa acagcagttg tgatgcttgt tgcagaaaat
     6241 gctatacaag caaaagctct tagaaatcaa aacaaacaaa ttgcagagct gagagcgatt
     6301 atacaacaaa acgtccaagc aataaataga gagtttgtaa ctgctaaaaa cacgatttta
     6361 gataaccaat tagctattaa gttaattggt gatgatgttg gtcacatcaa gcaaaattat
     6421 aagcggaagt ggcgaaaata aattttaaac tggtcgaatt cgaccccttt agaatcaaac
     6481 taagtcgtag gaatacggtt tttttattat gtccaagcat tgacgacgat aaaagctatg
     6541 gaattaataa tcggggacga tttaaaacat aggaggtgcc aatcatggca gaagaaacac
     6601 aagtagttga gatggttgaa gagcaagtgg tacctgaagc aaaacaaccg caagacgaaa
     6661 aaaagtatac agatgcagat gtggacgcta tcatcgacaa aaagtttgcg aaatggaagt
     6721 cagaacaaga agctgagaaa tcggaagcca agaaaatggc taagatgaac gagaaagaga
     6781 aagcagacta cgaaaagcag aagctgttag acgaattgca agaactaaaa aacgataaga
     6841 cacgcaatga gttaacagca gtagctcgtc aaatgtttgc agaatctgaa atcaacgtca
     6901 acgatgacgt acttggttta gttgtgactt tggacgcaga acaaacaaaa gcaaatgtaa
     6961 caacgctagc aaacgcattt gctaaagtta tcgctgatga ccgcaaggct cttgtacgcc
     7021 agaccactcc gtcaacaggc ggtggattga ggaaacaaac caattacggt gctaacttgg
     7081 ctagtaaggc agcacaacaa agcaccaagc ttttttagga ggaaattatg aacaaacgta
     7141 aagtaacaac atctaaagag attctacaca atctcgacta tgaagctatt tcagtaactt
     7201 tggattcaaa aaaaatcggc aaggaagttg ttccagctgg gacagtgtta gcaggtgttt
     7261 caggatcagt atttgaagac cgcaaacaga aagttaaagc ggtcgataac agtgccgtag
     7321 aaagtgagga taacatctgc gggattttgc ttacagatgt cgatttaaca aatggcgatg
     7381 cagtcggttc ctgtatttat cgtgggacta tcaatgcaga caagcttgct gattcatcta
     7441 ttgcgggaaa atatgaagtg ttggaagaag aactaccgca cattgtcttt atcaagggag
     7501 gtaaataaac atggcattga ttcacgaaat tatcacatcg gaaaatatca aaggttttta
     7561 caatgctaaa aacgaaaatg tcggaaacac gctaggggaa aacgcatttc ccccaaaaca
     7621 acaattagga ctcaaacttt cgtttattaa aggtgcagca ggcaaacctg tcacacttaa
     7681 agcggcagcc tttgatacaa aagtgccact acgtgaccgt atgactgttg aattactcga
     7741 cgaagaaatg ccattcttca aagaggctat ggttgtcaag gaagcagacc gtcaacaact
     7801 cagtttgtta gctcaaacta aaaatcaaga gcttattgac acagcgctag cagcgattta
     7861 caacgataaa gtaacactta ttgcaggtgc aaaagcacgt ctagaagcaa tgcgtatgga
     7921 agtattgtct aaaggtaaaa tccatgttgc atcaaacggt gtcatgaaag attttgatta
     7981 tgggttagat tcttcgcaaa caactaagcc agatacaaaa tgggaacaag tagataccgc
     8041 tacaccactt aaagacatcg aaaaagctat cgaagtaatg gcagaacgtg gctttgtgcc
     8101 agaagctatc atcatgaact ctaagacact tagcctgatt aaaaatgcaa gtaacacttt
     8161 agacgttgta aagcctatgg cgccagatgg ggcagctgtt actaaaggtg atttggaaac
     8221 ttatattgct gatgaattag gtcttaaaat tttgcttaag gatggaatgt ttgtcggaga
     8281 cgacggtaaa gctaaaaaat acttcccaga tggatttgca actttagttc ctaacggcaa
     8341 tcttggttac acagtatttg gtactactcc agagcaatca gatttgcttg gtggtgaggc
     8401 aacagatgct gaggtctcta ttgttgaaac aggtattgca attaccacta ctaagacaac
     8461 tgacccagtg aacgtccaaa caaaagtttc tatgattgct ttgccatcgt ttgaacgctt
     8521 ggaagaagta caaatcatta atgcaacaga atccgaagaa gaaactaaaa aagaaaacag
     8581 ctttgaaatg taggaggtca atatgcctag agtaattaga gcatttaaag ataaagtaac
     8641 aaaagtagtc tacgaagtcg gcgatattta ctcgggcgac cgagtagagt ttttgacaga
     8701 gggtggtgtt ttagaaccgt ccgtagactt tgacaagctg aaagtgagtg agattaaaag
     8761 caaacttgac gagttaagca ttgagtatga tgctaaactt aaaaaatccg agctattgaa
     8821 gcttttaaag caaacaatcg gatagtttgg aggtgtttat ggatgcagta aacacaagta
     8881 gcgttataag caatgtaaag cttgatttag gcatcttaga cagtcaacag gacgatttac
     8941 ttaacatgtt gctaaaacgc gttacagacc attttaaagc taaatatggc gctgtcgaaa
     9001 tagacagcgc ttttagtttt gttttagaag attgtttaat tgctagattt aaccgtagag
     9061 gtgccgagag ggcaaaaagc gagagtgtgg aaggtcatac gacaacatac tacgactttt
     9121 tggatgagtt tgaaccttac gatgccatga ttatggcaaa gcttaattta atcaaagaca
     9181 aatctcgtaa agggggactg tactttttat gagatatgca gatagagtta catttgttaa
     9241 aacgacggat gagcaataca atcccgattt aggtgagtat acgcacacag aggtcataag
     9301 tatcacaaaa ccttgttttg tgatggacat gggcatggaa aagtccgtac agatttttgg
     9361 agattatcaa aaggatcgta aagttatcta cctgaagcag ccttatacaa aagcatttga
     9421 ttattgcgag tatgagggca agagatacaa agcgcaggca aataagcttg gcgctattgt
     9481 tttttatctg gaaggagatg attctattgg tggctgatat atctttaaaa gtagttggaa
     9541 cagctggttt aaaaaagaaa cttgagctta ttgtcaaaaa agatgccgtc aagaagattg
     9601 tcagagacaa tgggacgcag cttcaaagga aaatgattaa taaagcggta tttacaaaag
     9661 gctattcaac aggtgcaact agacgttcta ttaccatgca aatcggcgat ggtggattga
     9721 gtgtcaaggt taaaccagga actcattatg ccggctacct tgaaagagga actcgcctta
     9781 tgagcaaaca accgtttgtt ttgccagctc taaaagagca aaaagtaaaa tttagaaaag
     9841 atttggaggc gcttgtcaaa tgattaaaac tagagatcag tctatttttg acgaaatgtt
     9901 taaacgcatc cagtctttag gttttaaggt ttacgattat aaaccgatga cggaagttcc
     9961 atatccattt gtggaaatgg aatctactga tgcggaatat attccaaata aagatgacat
    10021 taaaggttct gttgaactta tgttgtctgt ttggggggtg cagaaaaaac ggaagcaggt
    10081 gtctgacatg gcatctgcta ttttttcgca agctatgtcc gtagctcgta ccgatggatt
    10141 ctgttggtcg tttaatatta ggcagtcgtc tatacagatg ttagatgata caacaactgt
    10201 gacacctctt aaacgagcga ttgtcacact tagatttaat ttgagatagg aggtagtaat
    10261 gtcagaacct agcaaagcaa caacacagga ggagaaagaa gtaatgtcaa aagcacaaga
    10321 acaaacaaaa caattagaag caaaacaagg aattcattca atcttgttat ttcgtttgtt
    10381 gaaagaagca tctagtgagg tagcaactaa acttgctttt caaacagagc atgaagtagg
    10441 taaaagccgt gacgtagatg gacaaaaaac taaagatggt attatccagt ccgtgggggc
    10501 tttggagtac gactttaaag caacatctat tttagccaaa ggtgacgaac tagcagctaa
    10561 actagaaaaa gccatggagg atggtgagct tgtagaaatt tgggatattg attcagaaga
    10621 aataagcaaa aacggcgaca gcgacaataa gcttgcaaaa gtttggggta ttaataaaaa
    10681 tggaacaaac ggaggaaacg gtaaatgcct agcgacttat taccaagggt acatttcaag
    10741 cttcagcgct aagaaaaacg cagaagaaaa tattgtaatt gaaatggagt ttgccatcaa
    10801 tggtgttggt aaaaaagggt tcgctacatt aacagatgcc caaaaagcag ctgtacagta
    10861 cgcatttaaa gatacaacaa aatccaacag cgaagaaact aaaaaagaaa acagctttga
    10921 gatgtaacgg ttaggttgga tttagtatcc aaccttttat ttttaaagga gaaaagaata
    10981 atgcaattgg aaattaaagg taaaactcat aacgtaaaat ttggtacacg atttgttgcc
    11041 gagatggaca aaaatcatat agcagaacgt cagggattta aatttggagc tggtctacaa
    11101 tctagcgttc cgtttttaat cgaccacagc gttgttactc ttgcggaagt aatttataca
    11161 ggtacaatca ccgaaccacc acgtcctagt ttgaatgata tttacgacta tattgacgaa
    11221 gttgaagata ttgaaaagct ttttgatgat gttttagacg aactacgtca atcaaacgca
    11281 tcaaagttgt ttatggctca ggtggaaaaa gacctggcgg aagccaaagc gtaacaaata
    11341 taaaaaagag ttattcttct caagaaagct ttgagacgat tgtgcttaat tgcattagat
    11401 accttggcat gactgacatc aatgaaatcg ggcgattaac tttgtatgaa tatgatttat
    11461 taatgacagg caaagcacta gcggctgttg atgaatcaca taaagctcac aaacaagctt
    11521 ggataaacca ccaagttacg gcaacaaagc ttgttggtgg caagaaaaat aaaaaagaag
    11581 tccctgttta taaaaaattc aaggactttt ttgattatga ggaagaaatc cgaaaaatca
    11641 ctcaagaaat tgatgaaggt tacgacaaga aaggtatgga tttacttctc aaagctaacc
    11701 tttaaggaaa ggaggttaaa tgggagaatc ttattctgtt gaagcggttt tgacagctgt
    11761 tgataaaacc tttggcaaaa cattacaatc ggcaatccgt tcaatcgaag gcttggaaaa
    11821 gcgttcaacc ggtttttcat cggtgtctca aaaagctagt tccatgttta aatccatgtt
    11881 aggagcgaat ttagccggac aagctatctc ggcaatgaca aggacagtgt catcaggcct
    11941 tggctctatg cttggcgaga tgaatagttc agcgaaagcg tggaaaactt ttgacgccaa
    12001 tttagcggac attggatttg gaaaaaaaca aattttggca gctaaaacgg cgatgcaaga
    12061 ctatgcaact aaaacaatct actcggcatc agatatggct agcacgtatg cacagttagc
    12121 agcggttggc gtgaaagata ccggaaagct cgtaaaagct tttggcggtt tagctgcatc
    12181 tgctgaaaat ccgaagcagg ccatgaagtc tatcagtcaa caaatgacgc aagcagtagg
    12241 aagaccaaca gttgcatggc aagactttag gataatgctg gaacaggcgc ctgcagggat
    12301 ggctaaagtc gctaaatcta tgggtaaaaa tcttgatgaa ctcgtcgccg atatccaggc
    12361 gggtagggtt aaaaccagcg attttttgga agcggtaaaa aaagcaggca atgataagag
    12421 tttccaaaag atggcaactg agttcaaaac tgttgaccaa gccatcgacg gtatgcgaga
    12481 aggcttatcc aacaaattgc aaccagcgtt tgaaaaagtg aaccaatttg gaattagagc
    12541 gatcgaagca atcggtaaac aactcgataa agttgatttt tctaagtttg ctagtaatct
    12601 tgggaaattc cttgaaggaa ttaatatcga taaaattgta tctaatattt catcggcgat
    12661 ttcatctgtc acttcaaagg ttaaagaatt ttgggacggt ttcaaacaaa ctggagcaat
    12721 tagtgctttt tcaggagctt tgcagagcgt ttgggaagct ttaaaaaatg tcgctagcgc
    12781 catgagcgga ggaaattgga agacttttgg agcaacagtt ggagggactg ttaaacacgt
    12841 ctctaacttc gctaaagctg tttccgatgt tttaggaaag atggaccctg gcagactaag
    12901 aagttggata gctaccttcg ccgcagtagc tggaggcttt aaattattcg aaaaattaac
    12961 ggggcaaagc gtcattggtt cttttttgga taaaattggc agcaaatttg gcctctttgg
    13021 aaacaaagcc aaagaaggaa cagacaaagc ctctaacggc gctagaagaa gcggtggcat
    13081 tattagccaa atcttcagcg gcttgggtaa tatcgttaag tctgctggta cagccatatc
    13141 aacagctgca aaaggtatcg gagctggtat taaaactgct ttgtctggaa tcccccctat
    13201 cattagttct ctaggaaccg caatatcaac agttgcgcaa ggtataggca ctgggctagc
    13261 aatcgcattt aaaggccttg gttctgctat tgccatggtg ccgcctacca catggctagc
    13321 tttaggagcg gcaattttaa tggttggtgc tgcttttgcc ttggcaggaa ctcaagctga
    13381 tggcattagt caaattttaa gaaccgttgg cgatgttgtt gtgcaaatct tacaacaggt
    13441 cactgatagt ctagccactt tactacctat tatcgcaaac gctattggct ctatgttgcc
    13501 aattgtagct ggagctatct ctcagattgt aggcgcagta gcgggcggat tatctcagct
    13561 cgttatagcc gtttcaacag gggcatctct cgttataggg gctttcacag gacttcttgg
    13621 tggtatttct ggggttatta actccattag cgctgttatc caatcgctaa ctggtgtgat
    13681 taccgcagta ttcaatggca tagctactgt tatttcatct gtcggttcgg ctatcaaaga
    13741 tgtattgacg ggtctaggaa ccgcttttga aggatttgga aatggtgtaa aatcagctct
    13801 agaaggtgtt ggggcagtaa ttgaatcgtt tggtagtgca gttaggaatg tccttgacgg
    13861 tgttgcaaat atccttgatt ctatgggtac tgcggcactt aatgcaggcc gtggcgtaaa
    13921 agagatggct aaaggcatta aaatgcttgt tgatttatcc cttggagatt tggttgctac
    13981 attagcagct gtggcaagcg gtctagggaa gatggctagc tcagctggcg aaatgacaac
    14041 attaggttct gctatgagca aggtagccaa tggtatgaca cgtctagcaa caagtgctac
    14101 gatagcaatt actggattaa cagtctttgc caccaccatg gcaactatta agacagcagt
    14161 tgcaactcta ccgccagtcc taacgatggc agcgagtggg tttaccacat ttactactca
    14221 ggcggtggca gcagtgactg gattggctgc aattaatgct ccaatcacta tgtttaaagc
    14281 tcaactaatg acaataacac cagctctagc acaagctggc gctggctttg ccgcgtttgt
    14341 tgctcaatca tcaacattta gtacaggttt agcatctgcc ggtcctacaa tagcagcgtt
    14401 caatgctaat ttgatgagct tatctgcaac aacaggagcg ctagttgcat caatagctgg
    14461 tttatcagct gtgctttctg ttgtatcagc tggctttagc caaatagggg cttctgcgac
    14521 agcaactgtt ggtcaaatac aagcttttgc ttctagtaca acagttgttt cgtcagcatt
    14581 tgctagcatg caatctatga ttcaatctgc catggctgca atagtaagca gcattataac
    14641 atcatttaat caagcggcct ctcaaatgca atcaatctta tctcgaatgc tatctcaggc
    14701 taggacattt gggtctcaac tagagcaaca aatgagacaa tcgggacagc gttcaggaca
    14761 aaatcttgct cggggactat cttctcaaca aagtgctgtt attaatgcta tttctagcat
    14821 ggttaatgct gcggtatcaa gagccaacgc gggagctggt cctatgcgtc aagctggagc
    14881 gtacatcgga caagggcttg cgcaaggaat gtattcagcg ctaggagctg taacagctgc
    14941 agcaaacgcc cttgtagcac aagccgagag agcagcaaga gccaaggcga tgattcattc
    15001 gccgtcaagg ttgtttgcaa aacgagttgg tcaatatatc ccgcaagggg tagctatggg
    15061 tatcgacaaa aacgctgatg tcgttgacga ctctgttggc gggttatttg atagcatcaa
    15121 tagctttgat tttaatatcg cagatagact ggctagcatt ggagctaaat tccaaggtgt
    15181 tatcaaatca gagagttcac aatcgttatc gcagcaacaa gagttcgtac atacagctca
    15241 accagcgtat ataaacttta gtttaggtgg aaacgaatac gaagcatttg taagtgacat
    15301 cactaatcaa caagcaaaaa ttgaaaaaat cagactaaag agaagcagct ggtagttgtt
    15361 tctcttagtt ttttgaaagg agtaaaatgt acgaatttaa cgatactatc agaggtactc
    15421 cgaaagttac ttttaattta aagacaacaa ttggtgaaaa ggtattagaa gaagaactca
    15481 ataatgattt aggtaccttt aggacattga ctgtttctgg tcgtgatgtg gtggacttag
    15541 agcatcaaac aacaagtgtt ttagggcgaa atggtgagta tttccacaat gccacagtcg
    15601 aagtcagaaa actagaaata aaagctaaaa tcactggaaa agataaccag tcaatgcgtt
    15661 tgcaatatga aaaattaaac agattaattg ttagtcacaa tcaagttttt ttatcatttt
    15721 cagacgaacc tgacagaaat tatctaggca tctttaaatc taaagatgtc ccagaagaag
    15781 tttctaacga gcagattata ggactaacat tcatctgcta caatccgttt aaaatgtctg
    15841 atgtaaaaac taaaaaagga acatctatcc aaaatggtgg gttatttcaa acaaagccta
    15901 tcatcactct caacctatca tcaccaacaa aagaaattaa attgttgcat gtcgaaagcc
    15961 agaagtatat cagattgact ggaacttata ccactgacga aatcaagatt gatatggcca
    16021 cgggtaaaat tacccagaac ggtcgcaaca tccttggaga tttagatatg attaatagta
    16081 ggtattttga gctattacct ggtaataata cattacagtg tgccaatgcc gccataaccg
    16141 cagagtttag agaggtttat ctatgatata cctgtttgat aaacttgagc gattggtcgc
    16201 tactgttggt actgatgact tgctctcatg gcactttaag gtcaaaaaca atgattggga
    16261 ccaggctagt tttgaagtgc cagttgatta tgacgtagag ccatttgtct actttggttt
    16321 ttttaactac gaccctaatc aaaaagaaga tgtctttaag ctctttaaag tcattgatta
    16381 caacctagaa gatagcaagt tttacaaagg cttagacaaa gctgagagtg atcttgatac
    16441 cattgccatt atcaaagata agcgctttag acaatcgtcc gcagatgctt gtattgatgg
    16501 tgctttagag ggtacgggtt atcaagttgg caaagttgag ggaataacaa atgttagaac
    16561 acttagttat tactacatca gtccacgagc ggctctgatt aagattgtgg aagcttttaa
    16621 ctgcgagttc aatgtcaggt atacctttat taataacaaa atcactagtc gttatatcga
    16681 cctcaaaaag cgctttggaa agccaacagg caagcaattt gaacatggca acaacctgtt
    16741 aaaagtcgtc tacgaggaat caacagatga cattgtgact tgtctgattg gcagaggtaa
    16801 gggtgaggaa atccaacacg aagaagctga gcctaaagat gtcgagggac acttgccaca
    16861 ggaagaaagg cggcaaggct acggtcgaag aatcgaattt actgatgttg tctggtcggt
    16921 cgaaaaaggc gacccgatag acaaaccagc tggtcaaaac tttgtagcac tagatagtgc
    16981 aagggaagaa tacggcttgt ctcaaaatgg tgagctaaaa caccgctggg gtgtctttgt
    17041 caatgaggaa atcgaggata agacagaact cttaaaagca acttgggaag aattgcagcg
    17101 tttgtcaatc cctatcagaa tttacaaggc agaaatctta gacattggtc cagagacgtg
    17161 gaaaggcgac tcagtagcca ttatctatga tgaggtaaaa atagcttttg aaactcgggt
    17221 tgatgagatt gatattgaca agcttaattt taacaggtca gtcgtaacac ttggtgatta
    17281 cagcgttgtc caaaatcgtg agtcacggtc tcgtaaagag gctgtccaaa acatgataga
    17341 tgaatcttta gagactatca cagacttagg aatgacattt caggaatttt tgcaaggcat
    17401 cgaaaaacgc atcgagactg gcaaaaagga gatggaagac aattggcgaa aagttaacct
    17461 tgaattcgat aactttaaaa agaaggttga gcaagaaggc ttgcaattca acaccttgaa
    17521 agaacaaatc aaagaagttg atgaacgcac cgacaaagag ctagaggaat tccgagccac
    17581 cctcaaaaac ctagcgttac cagaggaagc gattaaaaaa atcacagagg ctatcaaagt
    17641 tgatgacatc ccgtctctta agcaaaactt tgatgactta aaaaacaagg tcagcgagac
    17701 aagcgagacg gcacgtctaa atgccgagat tatcggtaca
//
LOCUS       DATXQK010000032        16725 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.032, whole genome shotgun sequence.
ACCESSION   DATXQK010000032 DATXQK010000000
VERSION     DATXQK010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16725
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.032"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..1302
                     /locus_tag="VC394_001206"
     CDS             <1..1302
                     /locus_tag="VC394_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEP5994677.1"
                     /translation="SLLCRYGLTSAAALLLTFGGASAVKADEQEEKAKVRTELIQELA
                     QGLGGIEKKNFPTLGDEDLDHTYMTKLLTYLQEREQAENSWRKRLLKGIQDHALDGQD
                     GRNGERGEQGPTGPTGPAGPRGLQGLQGERGEQGPTGPAGPRGLQGERGEQGPTGLAG
                     KAGEAGAKGETGPAGPQGPRGEQGPQGLPGKDGEAGAQGPAGPMGPAGERGEKGEPGT
                     QGAKGDRGETGPVGPRGERGEAGPAGKDGERGPVGPAGKDGQNGQDGLPGKDGKDGQN
                     GKDGLPGKDGKDGQNGKDGLPGKDGKDGQDGKDGLPGKDGKDGLPGKDGKDGQPGKPA
                     PKTPEVPQKPDTAPHTPKTPQIPGQSKDVTPAPQNPSNRGLNKPQTQGGNQLAKTPAA
                     HDTHRQLPATGETTNPFFTAAAVAIMTTAGVVAVAKRQENN"
     gene            1488..1925
                     /gene="msrB"
                     /locus_tag="VC394_001207"
     CDS             1488..1925
                     /gene="msrB"
                     /locus_tag="VC394_001207"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEP5994678.1"
                     /translation="METSDELKQRIGDLSYEVTQHAATESPFTGEYDNFFEKGIYVDI
                     VSGEVLFSSLDKFNSGCGWPAFSKPIENRMVTNHDDSSYGMRRVEVKSREAGSHLGHV
                     FSDGPKEAGGLRYCINSAALKFIPYEQMEKEGYAQWLTLFDET"
     gene            2055..3074
                     /locus_tag="VC394_001208"
     CDS             2055..3074
                     /locus_tag="VC394_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEP5994679.1"
                     /translation="MSTHLRKLPGLLLCLLLALPAWYLGRLFPIIGAPVFAILLGMLL
                     ALFYHHRDKAKEGISFTSKYILQTAVVLLGFGLNLTQVMAVGMQSLPIIISTIATALL
                     VAYGSQKWLRLDVNTATLVGVGSSICGGSAIAATAPVIKAKDDEVAKAISVIFLFNML
                     AALLFPSLGQLLGLSNDGFATFAGTAVNDTSSVTATATAWDALHHSNTLDDATIVKLT
                     RTLAILPITLGLSLYRAKKEHDIVTEENFSLRKSFPRFILFFLLASLITTLMTSFGVS
                     ADVFHSLKTLSKFFIVMAMAAIGLNTNLVKLIKTGGQAILLGAICWVAITLVSLAMQL
                     SLGIW"
     gene            3281..3706
                     /locus_tag="VC394_001209"
     CDS             3281..3706
                     /locus_tag="VC394_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEP5994680.1"
                     /translation="MKRKFLIGSHGRLASGLQSSIDILAGMGQALETIDAYVDDSDYT
                     SQIDNFIAGVAADEQGLIFTDLLGGSVNQKMVTAVMNSGKDNIFLITNSNLATLLSLV
                     FLKPGEALTKDDIVTVINESQVQLVDLVPETNSEDDFFD"
     gene            3725..4216
                     /locus_tag="VC394_001210"
     CDS             3725..4216
                     /locus_tag="VC394_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEP5994681.1"
                     /translation="MITQIRVDDRLIHGQVAVVWTKELNAPLLVVANDEAAKNEITQM
                     TLKMAVPNGMKLLIRSVEDSIKLFNDPRAKDKRIFVIVNSVKDACAIAKEVPDLEAVN
                     VANVGRFDKSDPASKVKVTPSLLLNPEEMAAAKELVSLPELDVFNQVLPSNTKVHLSQ
                     LVN"
     gene            4233..5042
                     /locus_tag="VC394_001211"
     CDS             4233..5042
                     /locus_tag="VC394_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEP5994682.1"
                     /translation="MLVPATMAALAVLICFGGNYLTGQSMMERPLVVGLVTGLLLGDM
                     KVGILMGASLEALFLGNVNIGGVIAAEPVTATAMATTFTIISHIDQKAAMTLAVPIGM
                     LAAFVVMFLKNVFMNIFAPMVDKAAAANHQGKLVMLHYGTWIIYYLIIASISFIGILV
                     GSGPVNAFVEHIPQNLMNGLSAAGGLLPAVGFAMLMKLLWTNKLAVFYLLGFVLTAYL
                     KLPAVAVAALGAVICVISSQRDLELDAITRGAISKQTTFDSKESEEEDFFA"
     gene            5039..5866
                     /locus_tag="VC394_001212"
     CDS             5039..5866
                     /locus_tag="VC394_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEP5994683.1"
                     /translation="MTSQDNLTKEDRKMLRSVFWRSWTMNASRTGATQYHAVGVIYTL
                     LPVINRFYKTDKDKAEALVRHTTWFNATMHINNFIMGLVASMEKKNSEDPDFDASAIT
                     AVKASLMGPISGVGDSFFWGILRVIAAGIGISLASAGSAMGAVVFLLLYNIPAFIIHY
                     YSLYGGYSVGAGFIKKLYESGGIKIVTKTSSMLGLMMVGSMTASNVKFKTILTVAAKG
                     AKEAASIQDYLDQLFIGIVPLMVTLAAFWLLRKKVNINWIMFGIMFLGIILGLLGIC"
     gene            6002..7651
                     /locus_tag="VC394_001213"
     CDS             6002..7651
                     /locus_tag="VC394_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEP5994684.1"
                     /translation="MRGEQVEEHFKKQLQDDISRHFSYQSLMLSLLLIGLFIIFSLAP
                     QQLGLYRDINATATRYHRLISKQEALLDDLGKNSLLPFLNKNLSTADLSKHYFHLRHS
                     SQTSPELLLFSPSQDLLFASNPHLGNVFSKSVYIQEVLRAAHPPKTLFKVAMDSEDGH
                     YLMIIKPMIDQNQLKGYAFLVMSGKDFLHPTKTLTSELVIADKLDNTFTFSNREFIAS
                     SLDKINSQYLHHYFVFQDNRAFITRKVALQGGLWLYMYRPLIPMVSVMLFSLISSAVI
                     FVILQRKSSGLANRIAAKNSRAINQMVRDMSAISRQEKRRIDLESQDEFQYLSDQINQ
                     MVERLQQLHDKTLDLETQKLLFEKRMLEAQFNPHFLYNTLETILITSHYDSALTEKIV
                     IQLTKLLRYSLTDSSKPVLLKDDLSVIESYLVINQVRFEELQYSINLSPDLDSLAVPK
                     LFLLPLIENAIKYGLKERHDVKINIACYYQDDHIIFSVRDNGSGIDAHHQKVIREQLE
                     AGESHHGLINSYRRLKYHFSEVSLVFDQGDKQFNVSYHVKE"
     gene            7655..8443
                     /locus_tag="VC394_001214"
     CDS             7655..8443
                     /locus_tag="VC394_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP5994685.1"
                     /translation="MYKLVIIEDEHIIRKWLRYAIDYKALDILVIGEAKDGKEGAVLI
                     KESQPDIVLTDINMPIMTAFDMFEATKDQTYAKIILSGYADFPNARSAIHYGVLEFLT
                     KPIEKAALWECLQTIIAKIEKQKGSNQKTDACVYIPLPQMTDQIPEVVKDILEWVHAH
                     FQDKISTSRLAHDLGYSESYIYQNIKKHLQMPLSDYINQYRINQAIQLMQQEPDLMVY
                     EIAQAVGIYDYRYFDRVFKKYLGQTVKAFKEEHFMKDTDRSKPC"
     gene            8437..9483
                     /locus_tag="VC394_001215"
     CDS             8437..9483
                     /locus_tag="VC394_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEP5994686.1"
                     /translation="MLKLKRKWLLSFLLVIIILAIVNIAMYIFSSSKKDSAKELVILT
                     PNSQTILTGTIPAFEEKYGVKVRLIQGGTGQLIDQLGRKDKPLNADIFFGGNYTQFES
                     HKDLFESYVSPQVSTVISDYQLPSHRATPYTINGSVLIVNNELARGLHITSYEDLLQP
                     ALKGKIAFADPNSSSSAFSQLTNILLAKGGYTSSEAWEYVKRLLLSMNSIRATSSSDV
                     YESVAEGKMIVGLTYEDPCINLQKSGANVSIVYPDEGTVFVPSSVAIIKKAKAMKEAK
                     QFINFILSSDVQNAFGQSTSNRPIRRDAQTSHEMKPLDEIITLKEDYGYVTRHKKKIL
                     NTYNRLHEALERGK"
     gene            9596..10993
                     /locus_tag="VC394_001216"
     CDS             9596..10993
                     /locus_tag="VC394_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEP5994687.1"
                     /translation="MAYQTIYPYTNEVLHTFDNMTDQDLADVLERAHLLYKKWRKEDH
                     LEERKAQLHQVANILRRDRDKYAEIMTKDMGKLFTEAQGEVDLCADIADYYADKADEF
                     LMSTPLETDSGQAYYLKQSTGVILAVEPWNFPYYQIMRVFAPNFIVGNPMVLKHASIC
                     PRSAQSFEELVLEAGAEAGSITNLFISYDQVSQVIADKRVVGVCLTGSERGGASIAEE
                     AGKNLKKTTLELGGDDAFIILDDADWDQLEKVLYFSRLYNAGQVCTSSKRFIVLDKDY
                     DRFKELLTKVFKTAKWGDPMDPETTLAPLSSAQAKADVLDQIKLALDHGAELVYGGEA
                     IDHPGHFVMPTIIAGLTKDNPIYYQEIFGPVGEIYKVSSEEEAIEVANDSNYGLGGTI
                     FSSNQEHAKAVAAKIETGMSFINSGWTSLPELPFGGVKHSGYGRELSELGFTSFVNEH
                     LIYIPNKTNNSNTKV"
     gene            11095..12891
                     /gene="uvrC"
                     /locus_tag="VC394_001217"
     CDS             11095..12891
                     /gene="uvrC"
                     /locus_tag="VC394_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEP5994688.1"
                     /translation="MNELIKHKLELLPDSPGCYLHKDKEGTIIYVGKAKNLKKRVRSY
                     FRGSHDTKTELLVSEIVDFEYIVTESDTEALLLEINLIQKNMPKYNIKLKDDKSYPFL
                     KITNESFPRLVITRYIKKNDGLYFGPYPDSYTANEVKKLLDRIFPFKKCKNPINKVCF
                     YYHLGQCCAHTICHTDKAYWDRLIDDVKHFLNGKDDKIIEDLRSKMLAASEEMAFERA
                     AEYRDLISGIATMRTKQRVMSKDLQDRDIFGYYVDKGWMCVQVFFVRQGKLIQRDVNL
                     FPYYNDAEEDFLTYMGQFYQDKQHFIPKEVFIPEAIDEELVAAIVPTKIIKPKRGEKK
                     QLVALATKNARVSLQQKFDLLEKDIKKTSGAIENLGQLLKIDKPVRIEAFDNSNIQGT
                     SPVAAMVVFVDGKPSKKDYRKFKIKTVVGPDDYASMREVLFRRYSRVKKEGLQAPNLI
                     IVDGGVGQVNVAKDVIEKQLGLTIPVAGLQKNDKHQTHDLLFGNPLEVVPLPRRSEEF
                     FLLHRIQDEVHRFAVTFHRQVRRKNSFSSTLDHISGLGPKRKQLLLRHFKTITAIASA
                     TSEEIQALGIPKTVVEAIQQQITDNKNDRSSP"
     gene            13076..13678
                     /locus_tag="VC394_001218"
     CDS             13076..13678
                     /locus_tag="VC394_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEP5994689.1"
                     /translation="MKFLELNKKRHAIKTFNDQPVDYEDLRTAIEIATLAPSANNIQP
                     WKFVVVQEKKAELAKGLPLANKVQVEQAQYVVALFSDTDLALRSRKIARIGVKSLPDD
                     LIGYYMETLPPRFAAFNEVQTGEYLAINAGIVAMNLVLSLTDQKIASNIILGFDKSTT
                     NEILDIDPRFRPELLITVGYSDEKPEPSYRLPVDEVIERR"
     gene            13803..15212
                     /gene="pepV"
                     /locus_tag="VC394_001219"
     CDS             13803..15212
                     /gene="pepV"
                     /locus_tag="VC394_001219"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEP5994690.1"
                     /translation="MTTIDFKAEVDKRKEAMLADLVDLLRINSERDDRSADDQHPFGP
                     GPVKALEHFLAMAERDGYKTRNIDNYAGDFEFGQGDEVLGIFGHLDVVPAGSGWDTDP
                     YEPVIKDDRIYARGSSDDKGPTMACYYALKIIKELGLPVSKKVRFIVGTDEESGWGDM
                     DYYFAHNGLKNPDFGFSPDAEFPIINGEKGNITEYLHFAGDNKGAFVLHRFQGGLREN
                     MVPESATAVITAPHDLDVLEAALEQFLSEYGVKGSMKATDGKIEVTIIGKSAHGSTPE
                     AGINGATLLAKFLNQFTFEGAAKDYLHVAGEVLHEDFAAEKLGLAYTDDRMGALSMNA
                     GVFNFDSQSADNTIALNFRYPKGTDAATLKSGLEKLPGLTKVSLSEHEHTPHYVPMDD
                     ELVATLLAVYEKQTGLKGYEQVIGGGTFGRLLERGVAFGAMFPGDENTMHQANEYMPL
                     ENIYRSAAIYAEAIYELIK"
     gene            complement(15280..16656)
                     /gene="mnmE"
                     /locus_tag="VC394_001220"
     CDS             complement(15280..16656)
                     /gene="mnmE"
                     /locus_tag="VC394_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002888396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEP5994691.1"
                     /translation="MSITKEFDTITAISTPLGEGAIGIVRLSGTDALAIAQSVFKGKN
                     LEQVASHTINYGHIIDPKTGTIIDEVMVSVMLAPKTFTRENVVEINTHGGIAVTNEIL
                     QLLIRQGARMAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMTIAVKQLDGSLS
                     QLINDTRQEILNTLAQVEVNIDYPEYDDVEEMTTALLREKTQEFQSLLENLLRTAKRG
                     KILREGLSTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNIKGVPLKL
                     VDTAGIRETDDLVEQIGVERSKKALQEADLVLLVLNASEKLTDQDRALLNLSQGSNRI
                     ILLNKTDLEQKIELEQLPADLISISVLTNQNINLIEDRINQLFFDNAGLVEQDATYLS
                     NARHISLIEKAVQSLEAVNDGLALGMPVDLLQVDLTRTWEILGEITGDAAPDELITQL
                     FSQFCLGK"
BASE COUNT         5127 a         3099 c         3557 g         4942 t
ORIGIN      
        1 agccttcttt gtcgctatgg cttaacctca gcggctgctc ttttgctgac ctttggaggt
       61 gcaagtgcag ttaaggcgga tgaacaagaa gagaaagcta aagttagaac tgaattaatt
      121 caagagttag ctcagggact agggggtatt gagaaaaaaa attttccaac tctaggtgat
      181 gaagatttag atcatactta tatgacaaag ctattaacat acctacagga acgagaacaa
      241 gctgagaata gttggcgaaa aagactacta aagggtatac aagatcatgc ccttgatgga
      301 caagacggtc gaaacggtga aagaggggaa caaggaccaa caggtccaac cggacctgct
      361 ggtccacgag gtctacaagg tctacaaggt gaaagagggg aacaaggacc aacaggtccc
      421 gctggtccac gaggtctaca aggtgaaaga ggggaacaag gaccaacagg tctcgctggt
      481 aaagccggtg aagctggagc caaaggcgaa accggccccg ctggtccaca gggtccacgt
      541 ggtgaacaag gcccgcaagg tcttccaggt aaagatggtg aagctggtgc tcaaggccca
      601 gcaggtccaa tgggtcctgc tggtgagcga ggtgaaaaag gagaacctgg tacccaaggc
      661 gctaaaggtg atcgcggtga aaccggtcca gtaggtccac gtggtgagcg aggcgaagcc
      721 ggtcccgctg gaaaagatgg tgaacgtggt ccagtaggtc cagctggtaa ggacggccaa
      781 aacggccaag atggtcttcc aggtaaagac ggtaaggacg gccaaaacgg taaagatggt
      841 cttccaggta aagacggtaa ggacggccaa aacggtaaag atggtcttcc aggtaaagac
      901 ggtaaggacg gtcaagatgg taaagacggc ctcccaggta aagacggtaa agatggcctc
      961 ccaggtaagg acggtaagga cggtcaacca ggtaaaccgg ctcctaaaac accagaggtc
     1021 cctcaaaaac cagataccgc accacataca ccaaaaaccc ctcagatccc tggccaatca
     1081 aaagacgtga cacctgcccc tcaaaaccct tctaatagag gtctaaacaa accacaaaca
     1141 caaggtggta atcagctcgc aaaaacacca gcagctcacg acacacacag acaattgcca
     1201 gcaacaggcg aaacaaccaa tccattcttt acagcagctg ctgtagctat catgacgaca
     1261 gctggagttg tagctgttgc aaaacgtcaa gaaaacaact aatcctcttt ttgctaccta
     1321 actgatggaa agtaaaactc agagagaacc tcaaggttct ctcttttttg aatcaagata
     1381 gtagctctag gtataacgtc ttgtctgagg tgctgtgaac aaatcagtta ccactcacct
     1441 ctttttttgg taaaatgtaa gcactaagac atcaaaagga gttttccatg gaaacatcag
     1501 atgagttaaa acaacgcatt ggtgatcttt cttacgaggt cacccagcat gcagcaacag
     1561 aatctccctt tacaggcgaa tatgataatt tttttgagaa gggtatctat gtcgatattg
     1621 ttagtggtga ggtcttattt tcatcactgg ataaatttaa ttctggctgt ggttggcctg
     1681 ccttttcaaa acccattgaa aatcgtatgg tcacaaacca cgatgactct tcttatggga
     1741 tgagacgagt tgaggttaaa agccgtgaag caggatcgca tttggggcat gtctttagtg
     1801 acggccccaa agaagctgga ggcttgcgtt actgtatcaa ttcagcagct cttaaattca
     1861 ttccttatga gcagatggaa aaagaaggct atgcccagtg gctcacattg tttgatgaga
     1921 cataaccggg tttcaaaatg actgaaaaag aagcgtcagt gattcccaca agcactggat
     1981 tggtgaggcg tacgggtcaa ttttacaagc ctttgtaaaa ggtctataat agggaatgaa
     2041 aaaaggaggt tagtatgtca actcacttaa gaaaacttcc agggctgtta ctttgcttgt
     2101 tgttagctct tccagcctgg tatttagggc gcttatttcc cattattgga gcacctgttt
     2161 ttgctattct tttaggaatg ttgttagcct tgttttatca ccatcgtgac aaggctaaag
     2221 agggaattag ttttacatcc aagtatattt tacaaacagc agttgttttg cttggttttg
     2281 gattaaacct aacccaagtt atggcagtgg gcatgcagtc tttaccgatt atcatttcaa
     2341 ctattgcgac agctcttttg gtagcttatg gctcacagaa atggctgcgc ttagatgtca
     2401 atacagccac cttggtgggt gtagggtctt ccatttgtgg ggggtctgct attgcagcga
     2461 cggctcctgt cattaaggca aaggatgatg aggttgctaa ggcaatttca gtcatttttc
     2521 tctttaatat gttagcagct ttgctatttc caagtttagg acaattactg ggcttatcta
     2581 atgatggttt tgctactttt gctgggacag ctgttaacga cacctcttcc gtgactgcaa
     2641 cggccacggc ctgggatgcc cttcaccatt ccaatacact agatgacgca accattgtca
     2701 aattgactcg caccttggct attcttccaa ttactttagg tttatccctt taccgagcga
     2761 aaaaagagca cgacatcgtt acagaagaaa actttagcct taggaagtca ttccctcgtt
     2821 tcatcctctt ctttttatta gcttccctca tcacgacatt gatgaccagc tttggtgttt
     2881 ctgccgatgt tttccattcc ctaaaaacct tatcaaaatt ctttatcgtg atggctatgg
     2941 cggctatcgg tttaaataca aacctggtta aattaatcaa gacgggcggt caggctatcc
     3001 ttttaggagc tatttgctgg gtagctatca cccttgtcag tttagccatg caattaagtt
     3061 tgggcatttg gtaatcagac acgagaaatc agtttgactg atttcttttt ttgtatctta
     3121 tttgtgtaga attgtctaca ttttaaagag aatagtgcat tttaaaaacg ttttcatttg
     3181 ataaaataaa cctatcgtta aaccagttca gtaaagacaa ctggttgaga taagcaatta
     3241 ccgtttattc agcggttttt gttaatagag gaggatagga atgaaacgaa aatttctcat
     3301 tgggagtcac ggcagactag ccagtggttt gcaaagctct attgacattt tagcaggtat
     3361 gggacaggca cttgaaacca ttgatgctta tgttgatgat agtgactaca ctagccagat
     3421 tgataacttt atcgctgggg tagcagcaga tgagcagggc cttattttta cagatttgtt
     3481 aggcggcagt gtgaatcaga aaatggtaac cgctgtaatg aacagtggca aagacaatat
     3541 ctttttaatc acaaacagta acttagccac tttattgtcg ttggtgtttt taaaaccagg
     3601 cgaagcgctt actaaagatg atattgtaac cgttatcaat gaatcgcaag ttcagttagt
     3661 ggatttggtc cctgagacaa attcagaaga tgactttttt gattaatatc taggaggaag
     3721 acaaatgatt actcaaattc gtgttgacga ccgtttgatt cacgggcaag tggcagttgt
     3781 atggaccaaa gagttaaatg cgccactttt agtagtggct aatgatgaag ctgcaaagaa
     3841 tgaaatcaca caaatgacgc ttaaaatggc tgtgccaaac ggcatgaaac tgcttattcg
     3901 ttcagttgaa gactcgatta agctctttaa tgatccacgc gccaaagaca agcgtatttt
     3961 tgtgattgtc aattctgtta aagatgcttg tgccattgct aaagaagttc cagacttaga
     4021 agctgttaat gtggctaacg tggggcgttt tgacaaatca gacccagcaa gcaaagtcaa
     4081 agtgacacca agtcttttac ttaacccaga agaaatggca gccgcaaagg aattagtcag
     4141 tttaccagaa cttgatgtct ttaaccaagt cttaccatca aataccaagg tccatttgag
     4201 ccaattagtc aactaaagag aggtaaaaaa gtatgttagt accagcaaca atggcagctt
     4261 tagctgtatt gatttgtttt gggggaaatt atttaaccgg tcaaagcatg atggaaagac
     4321 ccctagtagt tggactggtc acaggtcttc ttttaggaga tatgaaggtc ggtatcttaa
     4381 tgggggcctc tctagaagct cttttcctag gaaatgtgaa tattgggggt gttattgcag
     4441 ctgagcctgt aacagcgact gccatggcaa caacctttac cattatttca catattgacc
     4501 aaaaagcagc catgacccta gctgttccaa ttggaatgtt ggcagccttt gtggttatgt
     4561 ttttgaaaaa cgtcttcatg aatatctttg ctccaatggt tgataaagct gcagcagcta
     4621 accatcaagg taaactagtg atgctccact atggcacttg gattatctat tacttgatca
     4681 ttgcctctat ttctttcatt ggtattttag taggtagtgg acctgttaat gcctttgtgg
     4741 aacacattcc tcaaaatctc atgaatggct tgagtgctgc gggaggcctt cttccagctg
     4801 tcggttttgc catgctgatg aaattattgt ggaccaacaa attagctgtt ttttacctat
     4861 tgggctttgt cttaacagct tatttgaaat taccagcggt tgcagttgct gccctaggag
     4921 cagttatttg cgtcattagt tcccaacgtg atctagaatt agacgcaatt actagaggtg
     4981 caattagtaa acaaacaacc tttgactcta aagaatcaga agaggaggac ttcttcgcat
     5041 gacatcacaa gataatttga ctaaagaaga ccgtaaaatg cttcgctcag tcttttggcg
     5101 ctcatggacg atgaatgcta gtcgtactgg ggcaacacaa taccacgcag taggggttat
     5161 ttacacttta ctaccagtca tcaatcgttt ttataagact gataaggata aagcagaggc
     5221 actggtccgt catacaacat ggttcaatgc caccatgcac atcaataact ttatcatggg
     5281 tcttgtggca tcgatggaaa agaaaaacag tgaagatcct gattttgatg ctagtgctat
     5341 caccgctgtt aaggcttcgt tgatgggacc tatatctgga gttggagatt ccttcttctg
     5401 gggaatttta agggttattg cggcaggtat cgggatttcc ttggcaagtg caggctcagc
     5461 catgggagct gttgtcttct tactcttgta caatattcct gcttttatta ttcactacta
     5521 tagcctttat ggtggttatt ctgtaggtgc aggatttatc aaaaaacttt acgaatctgg
     5581 tggtataaaa atcgtcacta aaacctcaag catgcttggg ttaatgatgg taggctcaat
     5641 gacggcttca aatgtaaaat ttaaaacgat tcttacagtt gctgcgaaag gtgccaaaga
     5701 agcagcttct attcaagatt accttgacca attgtttatt ggtattgtac cattaatggt
     5761 aacactagca gctttttggc ttctacgaaa gaaagtgaat atcaactgga ttatgtttgg
     5821 tatcatgttc cttggcatta tccttggact tttaggtatt tgctaataat ctcctagcaa
     5881 taaaggtaag taagacaagt tttcttgttt tgcttacctt tttcctttta agatgatgag
     5941 acttgttttg atgaaaaacg tagtagcatt aagaaatttg ttataatgaa aggagcttaa
     6001 gatgcgaggt gaacaggtgg aagaacattt taaaaaacaa ttacaagatg atatttctag
     6061 acatttttcg taccaatcgt taatgttatc attgttgcta attggtcttt ttattatttt
     6121 ttcattagcg ccacaacaat taggcctcta tcgtgatatc aatgccactg caacccgtta
     6181 tcaccgtttg attagcaaac aagaagcctt gcttgatgac ttagggaaaa atagcttact
     6241 acctttttta aataaaaacc tcagcactgc tgatttaagc aagcactatt ttcatttgcg
     6301 ccatagcagc caaacgtcac cagaactttt actattttca cctagtcaag accttttatt
     6361 tgctagtaac ccacatttag gaaatgtttt tagtaaatct gtttatattc aagaagtctt
     6421 gagagcagct catcctccaa aaaccttgtt taaagttgct atggatagtg aagatggtca
     6481 ctacttgatg atcattaagc caatgattga tcaaaaccaa ctaaagggat atgctttttt
     6541 agtgatgagt ggcaaagatt ttcttcatcc tacaaaaaca ctgacgtcag agttggtcat
     6601 tgctgataag ctggacaata cgtttacgtt ttccaatcgt gagtttatag catctagtct
     6661 agataagatc aacagtcagt atttacacca ttattttgtt tttcaagata atcgagcctt
     6721 tatcacaaga aaggttgcct tacaaggagg tctttggctt tacatgtata ggcccttgat
     6781 tccaatggta tcggtgatgt tattttcact tatttcatca gctgttatct ttgtgatctt
     6841 gcaacgtaaa tcaagtggtt tagctaaccg aattgcagct aaaaattcaa gagcaatcaa
     6901 tcaaatggtt agagatatga gtgcgatctc tcggcaagaa aaaagacgta ttgaccttga
     6961 gagtcaagat gaatttcaat atttatctga tcaaatcaat caaatggtag agcgattgca
     7021 acagttacat gataaaacgt tagatttgga aactcaaaaa ttattatttg aaaaacggat
     7081 gttagaagct caattcaatc cgcatttcct ctacaatacg ctagaaacca ttttgattac
     7141 gagccattat gattctgccc taacagaaaa aattgttatc caattgacaa aattgttgcg
     7201 gtatagtctc acggattcta gcaaacctgt cttgctcaaa gatgatttaa gtgtcatcga
     7261 gtcttattta gtgattaatc aggtgcggtt tgaagaattg caatatagca ttaacctatc
     7321 cccagacctt gatagtcttg cagtgcctaa attgttcttg ttacctttga tagaaaatgc
     7381 catcaaatat ggcttgaaag aacgacacga tgtcaagatt aacattgctt gttattatca
     7441 agatgaccat attatttttt ctgtgcgaga taatggttcg ggaattgatg ctcatcacca
     7501 aaaagtgatt cgagagcaat tggaagctgg agagtcacac catgggttaa ttaactctta
     7561 tcgtcgactt aagtatcatt tttcagaagt atccttggtt tttgatcaag gtgataaaca
     7621 gtttaatgtt agctaccatg ttaaggagta gtctatgtac aaattagtca ttattgaaga
     7681 tgaacatatt attaggaaat ggttgcgata cgctattgat tataaagctt tagatatttt
     7741 ggtcattggc gaggctaaag atggcaaaga aggagctgtt ttaatcaaag agagtcaacc
     7801 agatatcgtt ttaactgata ttaacatgcc catcatgaca gcttttgaca tgtttgaagc
     7861 aacaaaagat caaacttacg ccaaaattat tttatctggc tatgccgatt ttccaaatgc
     7921 aagatctgct atacactacg gtgtacttga atttttaaca aaaccgattg aaaaagcagc
     7981 tctttgggaa tgtcttcaaa ccattattgc taaaatagaa aagcaaaagg ggtctaatca
     8041 aaaaacagat gcttgcgtct atattccctt accccagatg actgatcaga ttcctgaagt
     8101 agtcaaagat attttggagt gggtacatgc tcattttcaa gacaagatca gcacgagccg
     8161 cctagctcat gacttagggt atagtgagag ttatatctat caaaatatca aaaagcatct
     8221 tcagatgcct ttaagtgatt acattaatca atatcggatt aatcaggcaa ttcaattgat
     8281 gcaacaagaa ccagatttaa tggtatatga aatagcacaa gctgtaggta tttatgacta
     8341 tcgctatttt gatagggttt ttaaaaagta tctaggacaa actgtaaagg cttttaaaga
     8401 agagcatttt atgaaggata ccgataggag taagccatgt taaaactcaa acgcaaatgg
     8461 ctattaagct ttttgttagt aataattatt ctagcaatcg ttaacattgc aatgtatata
     8521 ttctcatcgt caaaaaaaga tagtgctaaa gaattagtta tcttgactcc taatagccaa
     8581 actattttaa cagggactat tccagccttt gaggaaaagt atggggttaa agtaagatta
     8641 atccaaggtg ggacgggcca acttattgat caattaggtc gaaaagataa gccattaaac
     8701 gctgatattt tctttggtgg caattacact caatttgaaa gccataaaga tttatttgaa
     8761 tcctatgttt ctccgcaggt ttctactgtc atttcagatt accaattgcc tagtcatcgt
     8821 gcaaccccat atacgatcaa tggcagtgta ctgattgtta ataacgaatt agcaagagga
     8881 cttcatatta ccagttatga ggatttgcta caaccagctt taaaaggcaa aattgctttt
     8941 gctgacccaa atagctcatc tagcgctttt tcacaattaa caaacatctt actggctaaa
     9001 ggaggctata cgagctcaga agcttgggag tatgtgaaac gtttattact gtctatgaat
     9061 tctatcagag caacaagttc ttcagatgtt tacgaatctg ttgcagaagg taaaatgatt
     9121 gttggtttga cctatgaaga cccttgcatc aatctgcaaa aaagcggtgc aaacgtttcg
     9181 attgtttatc cggatgaggg gacagtattt gttccgtcat cagtcgctat tatcaaaaaa
     9241 gcaaaggcaa tgaaagaagc taagcaattt attaatttta ttttatcatc agatgtacaa
     9301 aatgcctttg ggcagtcaac tagtaataga cctatccgac gagatgctca aacgagtcat
     9361 gaaatgaaac cattagatga aattattact ttaaaagaag attacggata tgttactaga
     9421 cataagaaaa aaatattgaa tacctataat cgactacatg aagcattaga aagaggaaag
     9481 tagactctag gcttgttgtt gctatactag tagtgttaca aaatagcgta gtcatttttt
     9541 gaaatcgatt tcataatctg gtagactaag attatcacga aaaggagata atataatggc
     9601 ttatcaaact atttaccctt atacaaatga agtccttcat acatttgata atatgaccga
     9661 tcaagatttg gcagatgtcc ttgaaagagc tcaccttctc tataaaaaat ggcgcaagga
     9721 agatcattta gaagaacgta aagcccagct tcatcaagtg gctaatattc ttcgccgtga
     9781 ccgcgataaa tatgctgaaa tcatgaccaa agacatggga aaactgttca cagaagccca
     9841 aggtgaagta gatctttgtg ccgatattgc tgattactac gctgataagg cagatgaatt
     9901 tttgatgtct acgccactag aaacagattc aggtcaagct tactatttga aacaatcaac
     9961 tggtgttatt ctagctgttg aaccttggaa tttcccttac tatcaaatca tgcgggtctt
    10021 tgcgccaaac tttattgttg gaaacccaat ggttttaaaa cacgcttcta tttgcccacg
    10081 atctgcccaa tcctttgaag aattagttct tgaagctggt gctgaagcag gtagtattac
    10141 caatttgttt atttcttatg accaagtatc acaagtgatt gctgataaac gtgtggttgg
    10201 tgtctgcttg acaggttccg aacgtggagg agctagcatt gcagaagaag ctggtaaaaa
    10261 cttgaaaaaa acaacccttg aattgggtgg cgatgatgcc tttattatcc ttgatgacgc
    10321 ggattgggat caattagaga aggtgcttta cttctctcgt ctctacaatg caggtcaagt
    10381 atgtacctca tcgaaacgtt ttatcgtgct tgacaaagac tatgaccgtt tcaaagagtt
    10441 attaacaaaa gtcttcaaaa cagctaaatg gggtgatcca atggatcccg aaacgacgtt
    10501 ggcgccatta tcatcagctc aagctaaagc agatgttctt gaccaaatta agttggcctt
    10561 ggatcatggt gcggagttgg tttatggtgg tgaagcgatt gatcatccag gtcattttgt
    10621 catgccaacc attattgctg gcctcacaaa agacaaccct atttactatc aagaaatctt
    10681 tggcccagtc ggtgaaatct acaaagtgtc ttctgaagaa gaagcgattg aagtggcgaa
    10741 tgattctaac tatggccttg gaggtactat ctttagtagc aatcaagaac atgcgaaagc
    10801 cgtagctgct aaaattgaaa ctggtatgtc ttttatcaac tcaggctgga caagtcttcc
    10861 ggaactccca tttggtgggg ttaaacattc aggctatggc cgtgaattga gtgagcttgg
    10921 ctttacatct tttgtgaatg aacaccttat ctatattccg aataaaacga ataatagcaa
    10981 tactaaagtt tgatctagat tgttaaagaa gagtgtaacg ccttaataaa caatcaaggc
    11041 gttacactct tcttttttaa agtcaaaatt ttaaaaattt gataaactag taacatgaac
    11101 gaactgatta aacataaact agaactctta ccagacagcc ctggctgtta cttacataaa
    11161 gataaagaag gaactattat ctatgtcggt aaggctaaga atttgaaaaa acgcgtccgg
    11221 tcctattttc gtgggagcca tgataccaaa acagagttac tagtatctga gattgtagat
    11281 ttcgaatata ttgtaacaga gtcagatacc gaagcgcttt tgttggaaat taacttgatt
    11341 caaaaaaata tgcctaagta caacatcaag ttaaaagatg ataaatctta tccctttctc
    11401 aaaatcacaa atgagtcttt tcctcggtta gtgattacaa gatatattaa gaaaaatgat
    11461 ggattgtact ttgggcccta cccagattct tacactgcta atgaagttaa aaagttatta
    11521 gataggattt ttccatttaa aaagtgtaaa aatcctatca ataaggtctg tttttattac
    11581 catttaggcc agtgctgtgc tcataccatt tgtcatacag acaaggccta ttgggatcgt
    11641 ttaattgatg atgttaagca ctttttaaat ggtaaagatg acaaaattat tgaagatctt
    11701 cgttctaaga tgttagcagc ttctgaagaa atggcatttg aacgtgccgc tgaatatcga
    11761 gatttgattt caggtattgc caccatgcgc accaagcagc gggtgatgag caaggatttg
    11821 caagatagag atatctttgg ctattatgtt gataagggat ggatgtgtgt ccaagttttc
    11881 tttgtgcgtc aagggaaatt aattcagcgt gatgttaacc tttttcccta ttataacgat
    11941 gcagaagaag atttcttaac ttatatgggg caattttacc aagataaaca gcatttcatc
    12001 cctaaagaag tctttattcc agaagctatt gatgaagaac tggtagccgc tattgtgcca
    12061 accaaaatca tcaaacccaa acgcggtgaa aaaaaacaac tggtagcttt ggctactaag
    12121 aatgctcgtg ttagtctgca acaaaaattt gatttattag aaaaagatat caaaaaaacg
    12181 agtggtgcta tcgaaaattt aggtcagtta cttaagattg acaagcctgt gcgcattgaa
    12241 gcctttgata attctaacat tcaagggaca agtccagttg cggctatggt tgtttttgtt
    12301 gatggcaaac ctagtaagaa agattatcgt aaatttaaga ttaaaacagt ggtgggacca
    12361 gatgattatg ccagtatgag agaagtgctt tttcgaaggt acagtcgggt gaaaaaggag
    12421 ggactgcaag ctcctaactt gattattgtt gatggaggcg ttggacaagt caatgtggcc
    12481 aaagatgtga tagaaaaaca acttggactc actattcctg ttgcgggact tcaaaaaaac
    12541 gataagcacc agacccatga tttgcttttt ggaaacccac ttgaagttgt accgttgccg
    12601 cgccgttctg aagagttttt cttactgcat cggattcaag acgaagtgca tcgctttgca
    12661 gttacatttc atagacaagt gcgacgtaaa aattcttttt catcaacatt agatcatatt
    12721 tcagggcttg gcccaaagcg aaaacagtta ttactaagac actttaaaac cataacagcg
    12781 attgcatcag ctacctctga ggagattcaa gctttaggta tacctaaaac agtagttgaa
    12841 gcaatacagc aacagataac agacaataaa aatgatagat cgtctcctta aataagtgtt
    12901 atgttttcag tactgatgag gtaaaaaggc gctacttgtt acgcacgaca tttcagttcg
    12961 ttattttgaa actttcagaa agttgcgaag tgtagtgagt ttatgatttt aaaaactatt
    13021 ttatggtaaa ataaaatggt attaaaaaaa acagatttag ataggaagtt ttgaaatgaa
    13081 atttctagag ttaaacaaaa aacgccatgc cattaagacc tttaatgacc agccagttga
    13141 ctacgaggat ttgcgaacag ccattgagat tgcaacctta gcacctagtg ccaataacat
    13201 tcaaccttgg aaatttgtag tcgttcaaga gaaaaaagct gagttagcaa aagggttacc
    13261 tctagcaaat aaagtgcagg ttgaacaggc tcaatatgta gtggcgctat tttcagacac
    13321 tgatttagct ttacgttctc gtaaaattgc ccgtatcggt gtcaaatcgt taccagacga
    13381 cttaattggt tattatatgg agactttgcc accacgtttt gctgccttta atgaggttca
    13441 aaccggtgag tacttagcta ttaatgcagg cattgtggct atgaacttgg tgttatcctt
    13501 gacggatcaa aagattgctt ccaatattat ccttggtttt gataagtcaa cgaccaatga
    13561 aattttagac atcgatccgc gttttcgtcc agaattattg attactgttg gctatagtga
    13621 cgagaaacca gaaccaagtt atcgtttgcc tgttgatgaa gtcatcgaaa gaagatagag
    13681 tcaggacaat tagttaaatg taatgtccag atgcgctacg gagcgaccgc tattgttaga
    13741 ctgatactat tttggcttat tttaagttaa acattatccc taaaacgaat aaggagagta
    13801 taatgacaac cattgatttt aaagcagaag ttgacaaacg taaagaagct atgctagcag
    13861 atttagttga cctattacgc attaattcag aacgcgatga cagatcggcc gatgatcagc
    13921 atccctttgg gccaggtcca gttaaggctt tggagcattt cctcgcaatg gctgagcgtg
    13981 atggctacaa aacgcgtaat attgataact atgcaggtga ctttgaattt ggtcaaggtg
    14041 atgaagtact aggaatcttt ggccaccttg atgttgtgcc agctggtagc ggttgggata
    14101 cagaccctta tgagccagtg attaaagacg atcgcatcta tgcgcgtggg tcttcggatg
    14161 ataagggacc aaccatggcg tgctactacg ctctgaaaat cattaaagaa ttgggtcttc
    14221 ctgtgtctaa aaaagttcgt ttcattgttg gtaccgatga agaatctggc tggggggata
    14281 tggattatta ctttgctcat aatggcttga aaaatccaga ttttggtttc tcaccagatg
    14341 ctgaatttcc aattatcaat ggtgagaaag gaaatatcac cgaatacctc catttcgctg
    14401 gtgacaacaa gggcgctttt gtgcttcatc gcttccaagg tgggttgcgt gaaaacatgg
    14461 taccagaatc tgccacagct gtcattacgg caccgcatga ccttgatgtg ctagaagcag
    14521 ccttggagca atttttatca gaatatggtg ttaaaggctc tatgaaggct actgatggta
    14581 aaattgaagt aactattatt gggaagtctg ctcacgggtc aactccagaa gctggaatca
    14641 atggtgccac cttgctggct aaattcctga atcaatttac ctttgaaggg gctgctaaag
    14701 attatttaca tgtggcaggt gaagtgcttc atgaagactt tgcggcagaa aaactaggcc
    14761 ttgcttatac cgacgaccgt atgggagcct tgagcatgaa tgcaggtgtc tttaactttg
    14821 atagtcaatc agctgacaat accatcgcct tgaatttccg ttatccaaaa gggactgatg
    14881 cggcaacctt aaaatcagga ttggaaaaat taccaggttt aactaaggta agcctttctg
    14941 agcatgaaca tacacctcat tatgtgccaa tggatgatga attggtagca accttgcttg
    15001 ctgtttacga aaaacaaaca ggtcttaaag gctacgaaca agttatcggc ggaggaacct
    15061 ttggtcgttt gctagaacgt ggtgtggcct ttggagcaat gttcccaggt gatgaaaaca
    15121 ctatgcacca agctaatgaa tatatgcctt tagaaaatat ttatcgctca gcagctattt
    15181 atgctgaagc aatctacgaa ttaattaaat aagatgttta tgacagcaaa aagagctgga
    15241 acagtgttcc agctcttttg tgatgattag agactgttgt tatttaccaa gacagaattg
    15301 actaaagagt tgggtaatga gctcatcagg agcagcatcc cctgtgattt ctccgagaat
    15361 ttcccaagtt ctggttaaat caacctgtag taaatcaact ggcatgccaa gcgccaagcc
    15421 gtcattgaca gcttctaaac tctggactgc cttttcaatc aaagaaatgt ggcgggcatt
    15481 tgacaggtaa gtggcatctt gttctactag tccagcatta tcaaagaaga gctggttgat
    15541 acgatcttcg atcagattaa tgttctggtt ggttaacacc gaaatagaaa taaggtccgc
    15601 tggcaactgt tccaattcaa tcttttgttc caaatctgtt ttattcagca atataatgcg
    15661 gttactgccc tgactgagat ttaacaaagc ccggtcttgg tcagtcagtt tttcagaagc
    15721 attcagcaca agaagaacaa ggtcagcttc ttgaagagct tttttagacc gttcaactcc
    15781 gatttgttcg accagatcat ctgtctcacg aataccggcc gtatcgacaa gttttagagg
    15841 aactcccttg atattaacgt attcttcaat gacatctcgg gtggttcctg caatatcagt
    15901 cacaatagcc ttgtcttctc gcaacaaatt attaagtaag cttgacttac cgacgtttgg
    15961 acgaccaata atagctgttg ataagccctc acgcagaatt ttaccgcgtt tagctgttcg
    16021 aaggagattt tccaaaaggc tttggaattc ttgtgttttt tcacgtagca gagcagtggt
    16081 catttcttcc acatcgtcgt attcagggta gtcaatatta acttcgactt gagctaaggt
    16141 gttgagaatt tcttgtcggg tgtcattgat aagctgggaa agggagccat ccagttgctt
    16201 gactgcaatc gtcatagcct tgtctgtttt ggcacgaata atgtccatga cagcctcagc
    16261 ttgagtcaaa tcaacacgac catttaagaa ggcacgtttg gtgaattcgc caggctctgc
    16321 cattctagcg ccttggcgaa tcaatagttg caagatttca tttgtcacag caataccacc
    16381 gtgcgtattg atttcaacca cattttcacg ggtaaaggtt ttgggggcta gcatgactga
    16441 caccataact tcatcgataa tagttcctgt cttaggatca atgatatggc catagttaat
    16501 ggtgtgtgag gcgacttgct ctaagttctt tcccttaaaa acagattgtg caatggcaag
    16561 cgcatctgtc ccggatagac gaacaatgcc aatagctcct tcaccaagag gggttgaaat
    16621 ggcggtaatt gtatcaaatt ctttagttat actcatgccc tctattttaa atcaagtcct
    16681 agtagaaagc aaggtattat tttgtataga aaataggact agcac
//
LOCUS       DATXQK010000033        16197 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.033, whole genome shotgun sequence.
ACCESSION   DATXQK010000033 DATXQK010000000
VERSION     DATXQK010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16197
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.033"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(324..581)
                     /locus_tag="VC394_001221"
                     /pseudo
     CDS             complement(324..581)
                     /locus_tag="VC394_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770118.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            977..1546
                     /locus_tag="VC394_001222"
     CDS             977..1546
                     /locus_tag="VC394_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEP5994692.1"
                     /translation="MKGMNYQDYIWDLGGTLLDNYELSTQAFVQTLAFFSLPGDHDAV
                     YQKLKESTAIAVAMFAPNEPEFLHVYRLREADKLAQPIWCLGAKEILEKIATSGSRNF
                     LISHRDCQVNQLLEQAGLLIYFTEVVTASNGFARKPNPESLFYLKEKYDINSGLVIGD
                     RLIDKQAGQAAGFNTLLVDGRKNLLEIVT"
     gene            1547..3499
                     /gene="gyrB"
                     /locus_tag="VC394_001223"
     CDS             1547..3499
                     /gene="gyrB"
                     /locus_tag="VC394_001223"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEP5994693.1"
                     /translation="MIEENKHFEKKMQEYDASQIQVLEGLEAVRMRPGMYIGSTAKEG
                     LHHLVWEIVDNSIDEALAGFASHIKVFIEADNSITVVDDGRGIPVDIQAKTGRPAVET
                     VFTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVRVYKNGQIHYQEFKRGAVV
                     ADLEVIGTTDVTGTTVHFTPDPEIFTETTQFDYNVLAKRIQELAFLNRGLKISITDKR
                     SGMEQEEHFHYEGGIGSYVEFLNDKKDVIFETPIYTDGELEGIAVEVAMQYTTSYQET
                     VMSFANNIHTHEGGTHEQGFRAALTRVINDYAKKNKILKENEDNLTGEDVREGLTAVI
                     SVKHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFQRFLLENPQVARKIVEKGILASKA
                     RIAAKRAREVTRKKSGLEISNLPGKLADCSSNDANQNELFIVEGDSAGGSAKSGRNRE
                     FQAILPIRGKILNVEKATMDKILANEEIRSLFTAMGTGFGADFDVSKARYQKLVIMTD
                     ADVDGAHIRTLLLTLIYRFMRPVLEAGYVYIAQPPIYGVKVGSEIKEYIQPGIDQEDQ
                     LKTALEKYSIGRSKPTVQRYKGLGEMDDHQLWETTMDPENRLMARVTVDDAAEADKVF
                     DMLMGDRVEPRRNFIEENAVYSTLDI"
     gene            3867..5591
                     /gene="ezrA"
                     /locus_tag="VC394_001224"
     CDS             3867..5591
                     /gene="ezrA"
                     /locus_tag="VC394_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEP5994694.1"
                     /translation="MSSGIILLIVAIVLLVIIAYLVGVIIRKRNDSLITSLEERKQAL
                     FALPVNDEIEEVKSLHLIGQSQTSFREWNQKWVDLTVNSFADIENHIFEAENLNDTFN
                     FIRAKHEINSVESQLNLVEEDIASIREALNILKEQEEKNSARVTHALDLYEKLQASIS
                     ENEDNFGSTMPEIDKQMKNIETEFSQFVALNSSGDPVEASEVLDRAEEHTIALGQITE
                     QIPAIVAKLEDDFPDQLDDLETGYRRLLEENYHFPEKNIEARFQEIRESIRANSSELV
                     TLDLDRAREENTHIQERIDSLYEVFEREIAAYKVAAKNSKMLPRYLAHVKRNNEQLKD
                     EIARLSRKYILSETESLTVKAFEKDIKEIEDSTLAVAEQFGLQEKPFSELQVTFERSI
                     KTLTNVESGQMDVFAAVKDIEKIESQARHNLDVYVTQLHMIKRYMEKRHLPGIPQDFL
                     SAFFTTSSQLEALMDELSRGRINIEAVSRLSEVATVAIANLEDLTYQVVQNATLTEQL
                     LQYSNRYRSFEAGVQSSFEHALRLFEVENDYQASFDEISYALETVEPGVTDRFVNSYE
                     KTREHIRF"
     gene            complement(5723..6181)
                     /locus_tag="VC394_001225"
     CDS             complement(5723..6181)
                     /locus_tag="VC394_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEP5994695.1"
                     /translation="MTNLEDKLLKGAWGERRLNPEQQRYYLGTYAERVVLSALLDEAQ
                     TKTVKDYLKRELPSLQLVYQPLHLKISSKLAPQLQILYMKLAKENHLPVTIITETHMT
                     SPFAFILHTDHAINLKETRLEVILKQTKNDQLSKQPPEKTKSFWKRFLKK"
     gene            6408..7715
                     /gene="eno"
                     /locus_tag="VC394_001226"
     CDS             6408..7715
                     /gene="eno"
                     /locus_tag="VC394_001226"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEP5994696.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYLGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYTYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMIMPVGAPTFKEGLRWGAEVFHALKKILKERGLVTAVGDEGGFAPKFEGTEDG
                     VETILKAIEAAGYEAGENGIMIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQV
                     DYLEELVNKYPIITIEDGMDENDWDGWKVLTERLGKRVQLVGDDFFVTNTEYLARGIK
                     ENAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAG
                     QIKTGSLSRTDRIAKYNQLLRIEDQLGEVAQYKGIKSFYNLKK"
     gene            complement(8197..9225)
                     /locus_tag="VC394_001227"
                     /pseudo
     CDS             complement(8197..9225)
                     /locus_tag="VC394_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014677767.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(9505..11046)
                     /locus_tag="VC394_001228"
     CDS             complement(9505..11046)
                     /locus_tag="VC394_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEP5994697.1"
                     /translation="MLHLHLETKLQDKLSLLNILLDVSEVSIDQLCQETELKKQRVYN
                     LLFEMIKDLEDTLTLTICDDTVSIPYKTYQLKMPYFKKLYQTSIFLKMLCFLIEPGEL
                     SLHDFIKREYISQATAYRIRTNCRKYLKKVGLNVRQNHIVGPEYRIRFLIALLHYQFG
                     MTIYDFDKTSMNKVVSLIINSNQAITLNDASKAPYEFSYFAILISLIWKRRHDNLGIP
                     QTDAFKHLKKLSIYRDIKMTSQEIIGKWYHPDLTDEDLDYIFLCFCTTNNSFHKDKWT
                     PKKVKELFELVMTKTNGKTLKASLRPLLGNNILNSLPFKRILVSFSRLFISNLQVLLP
                     DIHLFHYLRRQQKRNKSFYNTLKTIVEEWMSAEGIVGKLPSYHLLLFTIQLEELLKTY
                     LPPIPVYLLTNNTAALDLMTNALSIYFPPAIATVMPVNVEIIPFKDIVKEKQSVIIAD
                     RQYLNLIQHLYQNQGHLFLYFLFSFRDVSEAYIHKAFLDFRQKRYDEFIVTLLDTYHK
                     NLSSP"
     gene            12402..>16197
                     /locus_tag="VC394_001229"
     CDS             12402..>16197
                     /locus_tag="VC394_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEP5994698.1"
                     /translation="MLKKIEVKEGQKNPISITLSEDQAKSLKNKEKLKVSIKQKQSKK
                     TSKDFFFEVGIDPKVKAKQQEKLLELDKVKKQIEDSINGDAWLPEKSEGEKPVQNTNK
                     ELQLQELNKKYQMAKEAIESATTLDDVETQFDKYTKVGDKDKYPDSLRNQYTRGDKDK
                     EIEKAKKSLGDLSDKVNGKIEEDKWLSDEVKKKQQQELEARKQKVNDSLKGSDSLKSL
                     RETVEKASSKNQKKPESFEDVYVPGNEETEKTKVRDILQKTYQKTEQNIETDPWLSPE
                     QKKAQKENAKARLDAGLKAVETTESLDKLKEVESDFLDKEKNPDSIPNQHKAGTPEVA
                     RKTFLDNFEKEAKKEIESIDKDDTLTANAKQVAKDKVAQQLQEATAKVEKAQSFDDLK
                     NIQDKFNSELPHTPGKPLKDQQSDAIAELEKKQQEIEKAIEGDKTLPRDEKEKQIADS
                     KERLKSDTQKVKDAKNADAIKKAFEEGKVDIPQAHIPGDLNKDKEKLLAELKQKADDT
                     EKAIDSDKTLTAKEKEEQKAKTKEELQKATEAVGAIDNREELDKKVPELKKAIEDTHV
                     KGNLEGIKNKAIEDLKKAHTETVAKINGDDTLDKATKEAQVKEADKALAAGKEAITKA
                     DDADKVSTAVTEHTPKIKAAHKTGDLKKAQVDANTALDKAAEKEREEINNDITLTAKD
                     KEQQLKEVETALTKAKDNVKAAKTADAINDARDKGVATIDAVHKAGQDLGARKSGQVA
                     KLEEAAKATKDKISADPTLTSKEKEEQSKAVNAELKKAIEAVNAADTADKVDDALGKG
                     VTDIKNQHKTGDPIEARRGLHNKSIDEVAQATKDAITADTTLTEAEKETQRGNVDKEA
                     TKAKEELAKAKDADALDKAYGDGVTSIKNQHKSGKGLDVRKDEHKKALEAVAKRVTAE
                     IEADPTLTPEVREQQKAEVQKELELATDKIAEAKDADEADKAYGDGVTAIENAHVIGK
                     GIEARKDLAKKDLAEAAAKTKALIIEDKTLTDDQRKEQLLGVDTEYAKGIENIDAAKD
                     AAGVDKAYSDGVRDILAQYKEGQNLNDRRNAAKEFLLKEADKVTKLINDDPTLTHDQK
                     VDQINKVEQAKLDAIKSVDDAQTADAINDALGKGIENINNQYQHGDGVDVRKATAKDD
                     LEKEAAKVKALIAKDPTLTQADKDKQTAAVDAAKNTAIAAVDKATTADGVNQELGKGI
                     TAINKAYRPGEAVKARKEAAKADLEKEAAKVKALIAKDPTLTQAAKDKQTAAVDAAKN
                     TAI"
BASE COUNT         5505 a         2682 c         3381 g         4629 t
ORIGIN      
        1 gacggctata tcacaacaga cgcagtaatg atcgtgcgca agaacggaca aatttttgat
       61 tatgtcttgc caggagagga agtcaggcca tgggagattg tgatcgagga gagggtggcg
      121 gaggtgttga tggaattgtg gtgaaagggt gcaaaaaagg ggcaaaaaag attaaaaatg
      181 aaactatttt aaacttttta ctataataat taccttttaa atcaacactt gaactattgt
      241 gaaccattgt gtacaatagt agtagattcc ggcaggggtc attattgtca ctgaaaaagc
      301 ctttacatca aaggcttttt gttttattcc ggttttaaaa ggggcataaa aggggcagtt
      361 tgtttattta taatttcttt catgtttaca gttgtgtgac tgtaaataga taatgttgtt
      421 tttggatcgc tgtgaccaac tctatccatt atcgcattta gcggtatccc tttttctgct
      481 aaaaatgata tatgcgagtg tctaaataag tgcgtgtgat aatctccgta aattttcaat
      541 cgcttattga tgtgtgcatt taaaattggt aaaccttttg aatatggaaa gctgttagca
      601 ctctgcttgt gattaatcta tcgtatgtta aaagtgacat ttaaattaaa tcatttttta
      661 tacatatatt tcaacaggat ttatcatatt ggaaaatagc ggtgcttcta ttggcatttt
      721 actattgaca ggaatttccc tgtcttttgt ctcctaaaga agatcagctt tgatttctaa
      781 cttgattaga gtgggcttgg tactgtttat gaattatcag tatatacttt ctggagatca
      841 aaaaggaaag gaacagctaa agaggtgtat gtgttaccat tagaggaata gaacatcgca
      901 agactcgttt attcagtcat tacgatacct gttttatttg ggaaataact ataatattaa
      961 gtgaatttgt gatataatga aaggtatgaa ttatcaagat tatatatggg atttgggagg
     1021 taccttactc gataattacg agctttcaac ccaagctttt gtccaaacgc tagctttttt
     1081 cagccttcca ggagatcacg atgctgttta ccaaaaatta aaagaatcaa ccgctatcgc
     1141 tgtggcaatg tttgctccta atgagccaga atttttgcat gtgtataggc taagagaagc
     1201 agacaaattg gcacaaccca tttggtgttt gggagctaaa gaaattttgg aaaagatagc
     1261 gacttcaggt tcgcgaaatt ttttaatttc tcatcgagat tgtcaggtga accaactttt
     1321 agagcaagct gggttgctaa tctattttac agaagtcgtg acagcttcaa atggctttgc
     1381 tcggaaacca aatcctgaaa gtttgtttta tttaaaagaa aagtatgata ttaatagtgg
     1441 attagtaatt ggagatcggc taattgataa acaagcagga caagcagcgg gctttaatac
     1501 cttgcttgtt gacggtagaa aaaacctatt ggagatagta acgtagatga ttgaagaaaa
     1561 taaacatttt gaaaaaaaaa tgcaagaata cgatgccagt caaattcagg ttctagaagg
     1621 gctggaggct gtccgcatgc gtccagggat gtatattggc tcgacagcta aagagggttt
     1681 gcatcattta gtctgggaaa ttgttgacaa ctcaattgac gaagcattag caggttttgc
     1741 ctctcatatt aaagtcttta ttgaagcaga taattccatt acagtagtag atgatggccg
     1801 tggaattcca gttgacatcc aagccaagac aggacgtccc gccgttgaaa cagtttttac
     1861 agtcttacac gcaggtggta aatttggcgg aggcggctat aaggtttctg gaggattgca
     1921 tggtgtaggg tcatctgttg ttaatgcttt atcaacacaa ttagatgtgc gtgtttataa
     1981 aaacggccaa attcattacc aagaatttaa acgcggggca gttgtagcag atcttgaggt
     2041 cattggaacc actgatgtga ctggaacaac ggtacacttt acacccgatc cagaaatttt
     2101 taccgaaacg actcagtttg attacaatgt tttagcaaaa cgtattcaag agttagcctt
     2161 tttgaatcgt ggtttaaaaa tttccattac agataagcgc tcaggtatgg aacaagaaga
     2221 acatttccat tatgaaggtg gaattggttc ttatgttgaa tttttaaatg ataaaaaaga
     2281 tgttatcttt gaaacgccca tctatacaga tggtgaatta gaaggtattg cagttgaagt
     2341 agccatgcaa tacacgacta gctatcaaga aacagtcatg agttttgcta ataatattca
     2401 tactcatgaa ggtggaacgc atgaacaagg ctttagagcg gctcttactc gggtcatcaa
     2461 tgactacgct aagaaaaata aaattcttaa agaaaatgag gacaatttga caggagaaga
     2521 tgttcgtgaa ggtttgacgg cggtaatttc tgttaagcat ccaaatcctc aatttgaagg
     2581 tcaaaccaaa acaaaattgg gcaactcaga agtggttaag atcactaatc gtctctttag
     2641 tgaggccttt caacgttttc ttttggaaaa cccacaagtt gctcgtaaga ttgtggaaaa
     2701 agggattttg gcttctaaag ctagaattgc agctaagcga gcccgcgaag tcacccgcaa
     2761 aaaatcaggc ttagaaattt caaacttacc tggaaaatta gcagactgtt cgtcaaatga
     2821 tgctaaccaa aacgaacttt tcatcgtcga aggagattca gcgggtgggt cggccaaatc
     2881 aggtcgtaac cgagagtttc aagctatctt gcctattcgc ggtaaaattt tgaacgtgga
     2941 aaaagcaact atggataaga ttcttgccaa cgaagaaatt agaagtcttt ttaccgctat
     3001 gggtacaggt tttggtgcag attttgacgt gtcaaaagct cgctaccaaa agctggttat
     3061 catgaccgat gccgatgtgg atggcgctca tattagaacc ttgcttttaa ccttgattta
     3121 ccgctttatg aggcctgttc tagaagctgg ctatgtttat atcgcccagc cacctattta
     3181 tggtgttaag gtcggtagtg agattaaaga gtacattcag ccaggtattg atcaagaaga
     3241 ccaattaaaa acagctcttg aaaaatatag tattggtcgt tcaaaaccaa ctgttcaacg
     3301 ttataaaggt cttggggaaa tggatgacca tcaactttgg gaaactacta tggatcctga
     3361 aaatcgtttg atggcgcgtg tgacagttga tgatgccgca gaagcagata aagtatttga
     3421 tatgttaatg ggagatcgtg ttgaaccaag acgtaatttc attgaggaaa atgcggttta
     3481 tagtacactg gatatttagt caccttttta gtttcacaag gaatctgcta ccaaagagga
     3541 cgaattgact aaaaatcatg atgcatgatg ccaacagctt tgttagttag tacgtcttca
     3601 tcttgaggga cagcgttaat gcataggtat tgagaccgcc ccttaatacc tatgttagtc
     3661 cttattaaga aagccttgtg agtcgctggt acgagattgt tgaattagtc aaatatattt
     3721 tgaaatgcat tttaaagtat gttataatcg taatgttata gtgtatttta aataaaattt
     3781 taataaaagg cgattgagga atttcagttt ttagttatta ctggaacaat gttgataaat
     3841 cgtgtctaaa taataaggag attcagatgt caagcggaat tatcttgctg attgtggcta
     3901 tagtcctact agtgattatc gcctatctag tgggtgtcat catacgaaag cgtaatgatt
     3961 ccctaattac cagtctagaa gaacgaaaac aggccttgtt tgctttgcct gttaatgatg
     4021 aaattgaaga agtaaagtca cttcatttga taggacagag ccaaacctct tttcgggaat
     4081 ggaatcagaa atgggtagac ttaactgtaa attcgtttgc agatattgaa aatcatattt
     4141 ttgaagctga aaatttgaat gatacgttta attttattcg cgccaagcat gagattaata
     4201 gcgttgaaag tcagcttaat cttgttgaag aagatatcgc ttctattcgt gaggcactta
     4261 atatcttaaa agagcaagaa gaaaaaaata gtgctcgtgt cacccatgct cttgatttat
     4321 atgaaaagct ccaagcttct atttctgaga atgaagataa ttttggttca actatgcctg
     4381 aaattgacaa acagatgaaa aacattgaaa ctgaattttc acagtttgtc gcactcaact
     4441 catcaggtga tcctgtagaa gcttctgaag ttctggatag agcagaagag catactattg
     4501 ctctagggca gattacagag cagattccag ctattgtcgc caagttggaa gatgattttc
     4561 cagatcagtt agatgatttg gaaacaggat atcgccgttt acttgaagaa aactatcatt
     4621 ttccagaaaa aaacattgag gcacgttttc aggagattcg tgagtctatt cgtgccaatt
     4681 catcagagtt agttacactt gacttggatc gtgctagaga agaaaacacg catattcaag
     4741 aacgcattga ttctctttat gaagtctttg agcgcgaaat tgccgcttat aaggtagctg
     4801 ctaaaaatag taagatgcta cctcgttacc tagcacatgt gaaacgtaat aatgaacaac
     4861 tcaaagatga aattgcacgt ttatcacgta aatatattct aagtgaaacg gaaagtctga
     4921 ctgttaaggc atttgagaag gatattaaag agatcgaaga cagtacactt gctgttgcag
     4981 aacaatttgg tttacaagaa aaaccatttt cagaactgca agtgactttt gaacgcagta
     5041 taaagacgct tactaatgtc gagtcaggtc aaatggatgt ttttgcagca gttaaagata
     5101 ttgaaaaaat tgaatcacag gcacgccata accttgatgt ttatgttact cagcttcata
     5161 tgattaaacg ttatatggaa aaacgtcact tgcctggaat tccacaagac tttcttagtg
     5221 ctttctttac aacgagttca cagttggaag ctttgatgga tgagcttagt agaggacgga
     5281 tcaatattga ggcagtgtca cgtttgtcgg aagttgcgac ggttgctatt gctaatctag
     5341 aagatttaac ctatcaagtg gttcaaaatg ctaccttgac tgaacaatta ctacaatatt
     5401 ctaatcgcta tcgttctttt gaagcaggag ttcaaagtag ttttgaacat gctttgagac
     5461 ttttcgaagt agaaaatgat tatcaggctt cttttgatga aatttcttat gctcttgaaa
     5521 cagttgaacc tggggtaaca gatcggtttg ttaactctta tgaaaaaaca cgagagcaca
     5581 ttcgtttttg atacttgaaa gtcgtgatat tctcacgact ttttattagc tttttggacc
     5641 aatacgtgtt tttgactaga atcttctttt taatgatcaa acgaagaagt agtaacagtg
     5701 ttttttggta ttgagaggat agttattttt tcaaaaatct tttccaaaag gatttggttt
     5761 tttcaggagg ttgtttgcta agttgatcat tttttgtctg ctttaagatg acttctaaac
     5821 gcgtttcctt tagatttatt gcgtggtcgg tatgaaggat aaacgcaaaa ggtgaagtca
     5881 tatgcgtttc tgtaataata gtaacaggta aatggttttc ttttgctaat ttcatataga
     5941 gtatttgaag ttgaggtgcc agcttggaag agatttttaa gtgtaaaggt tgatagacta
     6001 actgaaggct cggtaattct cttttcaaat agtcttttac tgttttagtt tgagcttcgt
     6061 ctaataaagc acttaaaacg actcgttcag catacgttcc tagatagtag cgttgctgct
     6121 caggatttaa tcttctttct ccccaggctc cttttaatag cttatcttct aaattagtca
     6181 tagataccct cgcttcttta taatagtttc tttattatat cgatttctac aataaaatac
     6241 caatttgaaa attttcggta aatgtgttaa aaaacaagaa agaaagtgat ttttcttgca
     6301 aaaaacaaag gaagcttttc atgagattgc tttttaagtt gattaaaggc ctagtttgtg
     6361 gtattatgaa catgtaaaat attaaaactc ataaggagag tattacaatg tcaattatta
     6421 ctgatgtata cgctcgcgaa gtccttgact cacgcggtaa cccaacactt gaagtagaag
     6481 tttatacaga atcaggtgca ttcggacgtg gtatggttcc ttcaggagct tcaactggtg
     6541 aacatgaagc cgttgaactt cgtgatggtg acaaatctcg ttaccttggt ctaggtactc
     6601 aaaaagcagt tgataacgtt aataacatta tcgctgaggc aatcatcggt tacgatgttc
     6661 gcgatcaaca agctatcgac cgtgcaatga tcgctcttga cggtactcct aacaaaggta
     6721 aacttggtgc taatgctatt cttggtgttt ctatcgctgt tgctcgtgca gctgctgact
     6781 atcttgaagt gccactttac acttaccttg gcggattcaa cactaaagtt cttccaactc
     6841 ctatgatgaa catcatcaac ggtggatcac actcagatgc cccaattgca ttccaagagt
     6901 tcatgatcat gccagttggt gcacctactt tcaaagaagg tcttcgttgg ggtgctgaag
     6961 ttttccacgc tcttaagaaa attcttaaag aacgtggact tgttacagct gtaggtgacg
     7021 aaggtggatt tgctcctaaa tttgaaggaa ctgaagacgg tgtagaaact atccttaaag
     7081 ctattgaagc agctggttac gaagctggtg aaaacggcat tatgatcggt tttgactgtg
     7141 catcatcaga attctacgac aaagaacgta aagtttacga ctacactaaa tttgaaggtg
     7201 aaggcgctgc tgttcgtaca tctgcagaac aagttgatta ccttgaagag ttggttaaca
     7261 aatacccaat tattactatc gaagatggta tggatgaaaa cgactgggat ggttggaaag
     7321 ttcttactga acgcctaggc aaacgtgttc aattggttgg tgacgacttc ttcgttacaa
     7381 acactgaata ccttgctcgt ggtatcaaag aaaatgcagc taactcaatc cttatcaaag
     7441 ttaaccaaat cggtactttg actgaaacgt ttgaagctat cgaaatggct aaagaagctg
     7501 gatatactgc cgttgtatca caccgttcag gtgaaactga agattcaaca atcgctgaca
     7561 tcgcagttgc aacaaacgct ggccaaatca aaacaggttc attgtcacgt acagaccgta
     7621 ttgctaaata caaccaatta cttcgtatcg aagatcaact tggtgaagtt gctcaataca
     7681 aaggtatcaa atcattctat aacttaaaaa aatagacggt aagacagaaa tcggtcattc
     7741 gtagaatgcg atttcgtcgt cgcacccccg tcaggctgac taggttggtc acgacaaaag
     7801 gagtgaacat ccagtcagct acgacgcaaa agttaaagat taaagaatag aaagcttggg
     7861 aaaatcccaa gctttttgct ttctaaatag taaaaattta ctgtgggcta atttatcctg
     7921 agctaatgta ttagcgaggt aaagttattg aggaaggctt ggatttgtac tgcctctaaa
     7981 aagttggaca ctatatttta aatacaggac taagtccttt tataccaatt gtcaagcaca
     8041 tcaaggtttt aactgaaaaa atgttttaga caatagccta ggtttagctg ttgtcttact
     8101 tgttgtgctc tgattagcat aaaaaagtgt acaagaaaaa aaccttgcct agtattttta
     8161 agatcaaggt gttacaatga tagagcaaca acgactttaa tgattttggg tcgaagcgta
     8221 attgtaaagt ttgttatgcg ttatgaggta aagcattgtc tgacaaggca atgtctagag
     8281 gcaatcgtat gtggcttggt tgaagctgtt tgcgattgcc tttttcgttt ctcatagaag
     8341 tctgcgatat gacaaggatt ggtatgacta gcaaaagcaa tgtcatggat acacttgaaa
     8401 agaatctttg aagcataagg attcccacgt ttggtaatgt gctcttgagc tagatagttg
     8461 ccagactcat aatgtctaaa gtcaatccca atgaaagcat taatttgatt agcagattga
     8521 aaacgacgaa tagcaccgag ttcgccaata atacttgttg cagttgtttc tgcgatactt
     8581 ggaatagagc gtagaatctt gtcttctggt aaaggttgag ccaacgccac catcttatca
     8641 aaaatagctt gacgacgttc agaaagtcta agtaattctt tggcatagta gcgaacctct
     8701 tctatcatcg gagaggtttt cgtaacagta aaataagact gcttggctaa tgagacaagt
     8761 ttttccacta agtagatccc acgcttgtca gagatacgtt tagaggtaga ttgacggatg
     8821 atagctgaca atgctttgtc agctagctcc agtacaaagc tattacatgg aaaagccgtt
     8881 accacattcc agtattactc accagttggt gcaaataaga tgtgttccag ttcaggaaaa
     8941 gtgacttgta agatcttgtg aaggcgattc ttaactctcg aacaatatta tcctcagtca
     9001 atttttgata gaagcgactc aagtcacgta gcagctgata aacttcttcc tgaacgtaag
     9061 ttggttttct aactagaata aattgagact gagccaattt ttcagtgtct aacttatccg
     9121 ctttacgcac ccgtagacta tctagttttc taaaggattg agttgtgtat aagcgtagcc
     9181 atttttctca aggaaggact gaagccgacg agaatagact cctgttgtga tggtctccct
     9241 tttgcctttt aaacaatagt atagccgttt ttcaagatgt tattaatgat tttttccttt
     9301 attctagaag aataagtctg atactgtttc ttcttaataa tctcaatgcc ctaacagtcc
     9361 atcagtcgaa cggtatttct caaattactg cagttgactc ttgcgttcag ttccctcacc
     9421 ttgtggatgc cgtttagaac aatattagct tgctgaatta gcttgctgag ccttttggta
     9481 atgtgaacaa agcatcttta tcactcaagg agaagacagg ttcttgtggt aagtgtccaa
     9541 caaggtcacg ataaactcat cgtaccgttt ttggcggaag tctagaaatg ccttatgaat
     9601 gtaagcctca gaaacgtctc taaaactaaa caagaaatac aagaacaaat gcccttgatt
     9661 ctggtacaaa tgctgtatca ggttcaggta ttgcctatct gcgataatga cactctgctt
     9721 ttccttaaca atatctttga aaggaatgat ttcgacattg acaggcataa ccgttgcaat
     9781 agctggcggg aagtaaatcg agagtgcatt ggtcatcaaa tccagcgcag cagtgttatt
     9841 ggtcaaaaga tagacaggta tcggtggcaa atatgttttt aggagttcct ctaactgtat
     9901 cgtgaagagc agtaaatgat aggacggcag tttccccacg atgccttctg cgctcatcca
     9961 ttcctcaacg atggttttta gagtattgta gaagctcttg tttcgttttt gctgtcttct
    10021 gaggtaatgg aacaagtgta tatcaggtaa caaaacttga agattactga taaatagacg
    10081 tgagaaggaa accaaaatcc gtttgaaagg aagggagttt aggatattat tgcctagtag
    10141 tggacgcagg gatgctttca aggtttttcc atttgttttt gtcatcacca actcaaacaa
    10201 ttctttcact tttttaggtg tccatttatc cttatggaaa gaattattgg tggtgcaaaa
    10261 acataagaaa atataatcca aatcctcatc agttaaatca ggatggtacc attttccgat
    10321 gatttcctga ctggtcattt tgatatctcg atagatagac agttttttca agtgtttgaa
    10381 tgcatctgtc tgagggatcc ccaagttatc atgtcgccgt ttccatataa gggatatcag
    10441 aatggcaaag tatgaaaatt cataaggggc tttactggca tcattaagtg tgatggcttg
    10501 attggagttt ataatcagcg aaaccacttt attcattgat gttttatcaa aatcataaat
    10561 ggtcatacca aactgatagt gcaacaaggc gatcagaaag cggatacggt attctggtcc
    10621 aacaatatgg ttttgccgaa cgtttaaacc tacctttttc aagtatttcc gacaattggt
    10681 cctgatacgg taagcggtgg cttgggaaat atactctcgt ttaatgaagt cgtgtaggct
    10741 cagctctcct ggttcaatta aaaaacataa catctttaag aatatcgagg tttgatagag
    10801 tttcttaaaa taaggcattt taagttggta ggtcttatag gggatagaaa cagtgtcgtc
    10861 acaaatggtt aaggtgaggg tgtcttctaa atccttaatc atttcaaaga gtaagttgta
    10921 gacacgttgt tttttgagtt cggtttcctg acacaactgg tctatggaga cttcggaaac
    10981 gtctaacaaa atgttgagca gtgataattt atcttgtaat ttggtttcta gatgaaggtg
    11041 taacataact gaactccttt catttttata gcagaaactc gtatttattt aattatcaca
    11101 taattaaata aaacagtcaa ataattcaat ggtgacgcgt gtttaaggag tattgagaat
    11161 gtcagatata agcaaaatgc agctgaatat tcttaatttt aggtatgaaa aagcgtcggg
    11221 attgataaaa aatctattga attgggtaaa tttaagttga tataataagt tgattagaaa
    11281 aattaataat tcatttaaat tctaatcagc tgtaacgttc cggggcgtgt taaactgtct
    11341 gaacatagaa aatacaggtt tctacggtga ctaaaataag ctaaggagga ttccttaaat
    11401 tctgtgatag gaacgtaaac tctctacaag agtagagcac gtcatcagtc agtctgggtg
    11461 tctactggct gacaacgtca aataagatag gcatgaaaaa ataaggggga atacagaatg
    11521 tttttaaaac accaagatgt gaaacaaaaa aattggagaa tgcgtaaggt caaaaaagtc
    11581 tttgttagtt catgtatgct tttaacagtg ggcctcggag ttgccgtact actgcattca
    11641 gccaatctaa tggcgtgatg gtcgtaaagg ctgcggaagc tgaagaactg ccagacaatt
    11701 taattgattt taaaggaact tgggaagtat ctgccgatgg ctcttctggt cgcttctatt
    11761 cagacggtgc tacaggtcaa tataagtttc accttattcc agcttcagat gttaaaaatc
    11821 cgggatggca tgaacataat aaagttaaag atagctatgt caaaataacc aaagagtcta
    11881 ttgcagctag atatacaaat aaaacgaaac ctccatattc tgtagcgttt aaggtcaaca
    11941 ctaaatcttt aataaaagat catgactata agataacttt tgagcaaggt tcaattgcgt
    12001 cgggaataac agttgattat cgtataggca gtgcttttaa taaaactaaa gatgattctt
    12061 tcaacatttc agatgagagt aaatatgctt ctaaggtaac aatagaaggt gaggaaagag
    12121 gttttataaa taggaaacca ggaactaaaa caatatcttt tagagctgta gaaaaaggaa
    12181 ctaaatctct tgtattgtta tctaaagtta atgaaaaaac ccaagtagat cttgatgtcg
    12241 agtttaaaaa cttcaaaatt attgatgtta ccaacccaag ccaattagat aaaggagttg
    12301 cttacgttgg aaacaaaaat gtagaattga cattaaaatc agacgatgga agaacaaact
    12361 tcgaaggtga tgagataagc ctttttaagc ctaatggaga tttgttaaag aaaattgaag
    12421 ttaaagaagg acaaaaaaat cctatatcaa taactttatc tgaagatcaa gccaaatctt
    12481 taaaaaacaa agaaaagtta aaagtaagca ttaaacaaaa gcaatcaaaa aagacttcta
    12541 aagacttttt ctttgaggta ggaattgatc ctaaagttaa agcaaagcaa caagaaaaac
    12601 tcttagaact agacaaagta aaaaaacaaa ttgaagactc aatcaatggt gatgcatggc
    12661 taccagaaaa atcggaaggc gaaaaaccag tacaaaatac taataaagaa ttacagttac
    12721 aagaactgaa caagaagtat cagatggcca aagaggcaat tgaaagtgct acaactcttg
    12781 acgatgttga aactcagttt gataaataca caaaagttgg agataaagat aaatacccgg
    12841 attctttacg taaccaatat actcgaggag ataaagataa agaaatagaa aaagctaaaa
    12901 aatcactggg tgatttaagc gataaagtga atggaaagat tgaagaagat aagtggctat
    12961 ctgacgaagt taaaaaaaaa caacagcaag aattagaagc gcgtaaacaa aaagtaaacg
    13021 actctcttaa aggatctgat agtttaaaat cattgagaga aacagtggaa aaagcttctt
    13081 ctaaaaatca gaaaaaacca gaatcttttg aagatgttta tgttcctgga aatgaagaaa
    13141 ctgaaaaaac aaaagttcgt gatatacttc aaaaaactta tcaaaaaaca gagcaaaata
    13201 ttgaaacaga tccatggttg agtccggaac agaaaaaagc tcaaaaagaa aacgctaaag
    13261 caagattaga tgcaggtttg aaagctgtcg aaactacaga aagcttagat aaacttaaag
    13321 aagttgagag tgacttcctt gataaagaaa aaaatccaga ttcaattcca aatcaacata
    13381 aagctggaac tcctgaagtt gctcgcaaaa ctttcttaga taactttgaa aaagaagcca
    13441 aaaaagagat tgaatctatt gataaagatg ataccttaac ggcaaatgct aaacaagtag
    13501 ctaaagataa agtggcacaa cagctacaag aagctacagc taaagtagag aaagcacagt
    13561 cgtttgatga tttgaagaac attcaagata agttcaattc tgaattacca cacacaccag
    13621 gtaagccgct taaagatcaa caatctgatg ctattgcaga attagagaag aagcagcaag
    13681 aaattgaaaa agctattgag ggtgataaaa cattaccaag agacgaaaaa gagaaacaaa
    13741 ttgctgactc taaggaacgc ttaaaatctg acacgcaaaa agttaaagat gctaaaaatg
    13801 ctgatgctat taaaaaagca tttgaagaag ggaaagtgga tattcctcaa gcacatatcc
    13861 caggtgattt gaacaaggat aaagaaaaac ttcttgcaga gttgaagcaa aaagcagatg
    13921 atactgaaaa agcgattgat tccgataaaa ctttgacagc gaaagaaaaa gaagagcaaa
    13981 aagcgaaaac aaaagaagaa cttcaaaaag caacagaagc tgttggagca attgacaatc
    14041 gtgaagaact agataaaaaa gtcccagaac ttaagaaagc tattgaagac actcacgtta
    14101 aaggtaatct tgaaggtatt aagaataagg ctattgaaga tcttaaaaaa gctcatactg
    14161 aaacagttgc taaaataaat ggtgatgata cccttgacaa agctactaaa gaagctcaag
    14221 tgaaagaagc tgacaaagct ttggcagcag gtaaagaagc tatcactaaa gcagatgatg
    14281 ctgataaagt aagtacagct gttacagagc acacaccaaa aattaaagca gcacataaaa
    14341 ctggtgatct taaaaaagct caagtagatg ctaacacagc tcttgacaaa gcagctgaaa
    14401 aagaacgtga agaaatcaat aatgatatca cattaacggc aaaagacaaa gaacaacaac
    14461 tgaaagaagt tgagacagct cttactaaag ctaaagataa cgtgaaagct gctaagacag
    14521 cagacgctat caatgacgca cgtgataaag gcgtagcaac aattgatgcc gttcataaag
    14581 caggtcaaga cttaggtgct cgtaagtcag gtcaagtcgc taaacttgaa gaagcagcta
    14641 aagcaacgaa agacaagatt tcagctgatc caactttgac aagcaaagaa aaagaagagc
    14701 aatctaaagc tgttaacgct gaacttaaga aagcgataga agctgttaac gcagctgaca
    14761 cagctgacaa ggttgacgat gctcttggta aaggtgtcac agacatcaag aaccaacata
    14821 agacaggtga ccctatcgaa gctcgtcgtg gattacacaa caagtcaatc gacgaagtgg
    14881 cgcaagcaac taaggacgct atcacagcag atacgacttt gactgaagct gaaaaagaaa
    14941 cacaacgtgg caatgttgat aaagaagcaa ctaaagctaa agaagaactt gctaaggcta
    15001 aagatgctga tgctttagac aaagcgtatg gtgacggtgt aaccagcatc aagaaccaac
    15061 acaagtcagg taaaggtctt gacgttcgta aagatgagca caagaaagct cttgaagctg
    15121 ttgctaaacg tgtcactgct gaaattgagg ctgatccaac cttaacacca gaagtgagag
    15181 aacaacaaaa agcagaggtt caaaaagagc ttgaacttgc gactgataag attgctgaag
    15241 ctaaagatgc agatgaagca gacaaagctt acggtgacgg tgtcacagcg atcgaaaatg
    15301 cccacgttat tggtaaaggt atcgaagctc gtaaagacct tgctaagaaa gaccttgctg
    15361 aagctgctgc taagacaaaa gctctcatta ttgaagacaa aacgcttact gatgatcaac
    15421 gtaaagaaca gttattaggt gttgatacag agtatgctaa aggtatcgag aatattgatg
    15481 cagctaagga tgctgcaggt gttgataaag catatagtga cggtgttcgt gacatcctgg
    15541 cacagtacaa agaaggtcaa aaccttaatg atcgtcgtaa tgctgccaaa gaatttcttc
    15601 ttaaagaagc agacaaagtg acgaaactaa tcaatgatga tccaaccttg actcatgacc
    15661 aaaaagttga tcaaatcaac aaagttgaac aagctaagtt agacgcaatc aagtctgttg
    15721 atgatgctca aacagctgat gctatcaatg atgctcttgg taagggtatt gaaaacatca
    15781 acaaccaata ccaacatggc gatggcgttg atgttcgtaa agcgactgcc aaagacgatc
    15841 ttgaaaaaga agctgctaaa gtgaaagctc ttattgctaa ggatccaacc ttgactcaag
    15901 ctgataaaga caagcaaaca gcagcggttg acgcagctaa gaacacagca attgcagcgg
    15961 ttgataaagc gacaacagca gatggtgtta accaagaact tggtaaaggc atcacagcta
    16021 tcaacaaagc ttaccgtcca ggtgaagctg ttaaagcacg taaagaagcc gctaaagctg
    16081 atcttgaaaa agaagctgct aaagtgaaag ctcttattgc taaggatccg accttaactc
    16141 aagctgctaa agacaaacaa acagcagcgg ttgacgcagc taagaataca gcaattg
//
LOCUS       DATXQK010000034        15997 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.034, whole genome shotgun sequence.
ACCESSION   DATXQK010000034 DATXQK010000000
VERSION     DATXQK010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15997
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.034"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..363
                     /locus_tag="VC394_001230"
     CDS             <1..363
                     /locus_tag="VC394_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEP5994699.1"
                     /translation="AIISIKPQFVDEIMKGNKRFEFRKSFLKSIPDRCYIYSTKPVGK
                     IVGFFTIKQVLRDEPEKIWQKTSKKSGITKDFFDEYYSGKNKAVAIEIDRLFAYKTPK
                     NYHEIDIKGKPPQTYKTI"
     gene            414..887
                     /locus_tag="VC394_001231"
     CDS             414..887
                     /locus_tag="VC394_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994700.1"
                     /translation="MANEENLIPGNKRSKSELREITRKGGIASGKARRRKKELKTIIE
                     QALNSVIPNEKAQKKLESLGFDPTFQSAIALKVVEQAMNGNLRAVELISNISFAGKDS
                     LDRKEQRQRIKAAELTTDEQRTRIELLKVKLDAEKGAKPDTSLMRALLDAVEGGD"
     gene            970..2181
                     /locus_tag="VC394_001232"
     CDS             970..2181
                     /locus_tag="VC394_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEP5994701.1"
                     /translation="MTPRSGKTTAGHFRYARYLIESEDENHLVTAYNQEQAYRLFIDG
                     DGTGLMHIFDGNCEIKHDERGDHLLITTPKGNKRVYYKGGGKVNSVGAITGMSLGSVV
                     FCEINLLHMDFIQECFRRTWAAKLRYHLADLNPPAPQHPVIKDVFDVQNTRWTHWTMD
                     DNPILTAERKQNIINSLKKNPYLYKRDVLGQRVMPQGVIYGLFDTEKNVLDALIGEPV
                     EMYFCADGGQSDATSMSCNIVTRVRDNGRISFRLNRVAHYYHSGADTGQVKAMSTYAL
                     ELKVFIDWCVKKYQMRYTEVFVDPACKSLREELHKLGVFTLGAPNNSKDVSSKAKGIE
                     VGIERGQNIISDGAFYLVNHSEEEYDHYHFLKEIGLYSRDDNGKPIDKDNHAMDEFRY
                     SVNVFVHRYYN"
     gene            2195..3697
                     /locus_tag="VC394_001233"
     CDS             2195..3697
                     /locus_tag="VC394_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEP5994702.1"
                     /translation="MGVIQKIKNLVTRSKYVMTTQSLTNITDHPKIAISKLEYDRITT
                     NLKYYKSDWDSVLYLNTDGETKKRDLNHLPIARTAAKKIASLVFNEQAEIKVDDDAAN
                     EFISETLKNDRFNKNFERYLESCLALGGLAMRPYVDGDKVRVAFVQAPVFLPLQSNTQ
                     DVSSAAVVIKSVKTINGKEVYYTLIEFHEWQSSDDYVISNELYRSDDKAKVGSRVPLS
                     EVYKDLKDEAKVTDVTRPIFTYLKTPGMNNKDINSPLGLSIFDNAKTTIDFINTTYDE
                     FMWEVKMGQRRVAVPESLTALTVRTTDGDVVPRPRFESDQNVYIRMGGRDLDSSAIQD
                     LTTPIRADDYIKAINEGLSLFEMQIGVSAGLFSFDGKSMKTATEIVSENSDTYQMRNS
                     IVALVEQSLKELVISIFEIAKAYDLYQSEVPSMDNISISLDDGVFTDRDAELDYWIKV
                     VNAGFGTREMAIQKVLNVTEEKAQEIAAEINTGIVDEINQQRTDTHLYGE"
     gene            3702..5180
                     /locus_tag="VC394_001234"
     CDS             3702..5180
                     /locus_tag="VC394_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor capsid protein"
                     /protein_id="HEP5994703.1"
                     /translation="MKKKPIKLNDEQLLLEASQLSDMYHQLTLDLFDQVIERIKARGS
                     ASLADNPYLWQANKLHDVGLLNADNIKLIAKYSGIAEAQLRYIIKNEGFKIYKNTSEQ
                     LEEALGRESGVNSTIQDDLSNYARQAIDDVHNLTNTTLPFSVIGAYQGIIQDAVAGVV
                     TGLKTPDQAINQTVIKWFKKGFYGFTDKAGRKWRADSYARTVINTTTWRVFNEVKEAP
                     AREFGIDTFYYSKKATAREMCAPLQHQIVTTGEAREEGGIKILALSDYGHGEPDGCLG
                     INCKHTKTPFVVGVNSKPELPEHLKNITPAQAKANANAQAKQRAIERSIRKSKELLHV
                     AKQLGDKELIRQYQSDVRSKQDALNHLVNSNDFLIESKSRSKMFVTDLMKREIVMKKG
                     LINDIIGLQTSDGITIKEISGHLLERIYERGVSESHIATALANPIYIRPDAVDGGRKV
                     SRRYVGTHVTVNINPHTGKIITTWKTGERTRRKYDNQRNVDK"
     gene            5152..5391
                     /locus_tag="VC394_001235"
     CDS             5152..5391
                     /locus_tag="VC394_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994704.1"
                     /translation="MTIREMLISNQILNNDDIDFMLSVNQDVSPLFELEDNDDNWDVL
                     VDELQQTSSYLTGDGESVSDRTKKFDDLVDKISAI"
     gene            5453..5719
                     /locus_tag="VC394_001236"
     CDS             5453..5719
                     /locus_tag="VC394_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994705.1"
                     /translation="MNKRIKKKRKLETAVVLLIAENARQAEAIKNQNKQIMELKSIVQ
                     RNALATNEELATVKAATLDNQSVIKAIGDTVDYIKKNYKRKWGK"
     gene            5845..6459
                     /locus_tag="VC394_001237"
     CDS             5845..6459
                     /locus_tag="VC394_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994706.1"
                     /translation="MHMENVTNENVDQETTDLENNQQEDKAFTQDDLNRVGTKEHSKG
                     YNKAVKDLGFDDVESAKDALKAFKEWKESQKSESEKQTEILNAKDKELEEARANNKAL
                     NAKLAAMSLGVNAESIDDVIALSERLVTDETSIEDAIKTVLGKYPHFGQTKDKAPKIT
                     VAGNPSADNGQGSVSKEDFAKMSYQEKLDLKLKNKNLYDQLKGN"
     gene            6463..7281
                     /locus_tag="VC394_001238"
     CDS             6463..7281
                     /locus_tag="VC394_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HEP5994707.1"
                     /translation="MAVGTTKMAQMLDPEVLADMIDAEVGKAIRFAPLAEVDTTLEGQ
                     PGTTLTVPKWDYIGDAEDVAEGEAIPMTQLGFKKTTMTIKKAGKGVEITDEAILSGYG
                     DPVGQAAKQIVEAIDHKVDADVLDALSKSTQTVEATATVDGVSKALDIFNDEDDAETV
                     IVMNPADASTLRLDAAKEWLGATEVGANRVVSGVYGEVLGVQIVRSRKCPKGTAYMVR
                     KGALRIMLKRNTMVETDRDITKAINQIVANKHYGVYLYKAEKAVKITLKDAAKK"
     gene            7335..7751
                     /locus_tag="VC394_001239"
     CDS             7335..7751
                     /locus_tag="VC394_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994708.1"
                     /translation="MEVVIIAFLTQKEFEDLGFDDVEDFEKMEKRASHAVNLYCRNRY
                     DYKDLKKEIALVQKAVKRAIAYQIAYLNDSGVMTAEDKQSFAGISLGRTSISYTVGHG
                     QGSQQKTLADRFNLCLDAENELLVVGLGYTGISYDR"
     gene            7741..8073
                     /locus_tag="VC394_001240"
     CDS             7741..8073
                     /locus_tag="VC394_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEP5994709.1"
                     /translation="MIDKRLLIDELQVKLVKDKGDYGGFVYDEPFTLSPVRFDRNLAT
                     AGKDNARQETKPSVIFIYPKYCKTVADRSWVDAVVIDGDTEYTVDKVIPVYHPLTNKI
                     FCFEVEVI"
     gene            8073..8429
                     /locus_tag="VC394_001241"
     CDS             8073..8429
                     /locus_tag="VC394_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEP5994710.1"
                     /translation="MAKVVVELGGIKRKVSPQALAKGKLIMNNQVMMSMNPYVPYRDG
                     ALRGSSRANSVGVTWSGPHARAQFYGGAYNKYKSFKFKKYTTPGTGKRWDKRALANAT
                     IVKDWEKSLLRGMGFK"
     gene            8426..8824
                     /locus_tag="VC394_001242"
     CDS             8426..8824
                     /locus_tag="VC394_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEP5994711.1"
                     /translation="MTNDFATVLRQFVEGLDLGIKPRLDYLTRQEDLAIYPMPGGKVN
                     NEYMDGTREISLPFEIAIKTKNQELASTVMWTINSALSNFDLKLPSLNHSYTFISLDV
                     EKPFLNDLSDQGFYIYVLDITAHLEIEGNN"
     gene            8824..9309
                     /locus_tag="VC394_001243"
     CDS             8824..9309
                     /locus_tag="VC394_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEP5994712.1"
                     /translation="MRQKNALRGHFIAPYVKGEEKTEVTKEKLLELARWIKDISDDTD
                     EKTEDEAYYDGDGTEETTVVGVKGAYTFEGTYDPEDKAQAHIASLKYKLGDERKVWHL
                     IVSADGKTQWLGVATVTEIIAGSGAAADFEAFGCKITYNSLPKESKEIIPKKNELSVA
                     M"
     gene            9348..9782
                     /locus_tag="VC394_001244"
     CDS             9348..9782
                     /locus_tag="VC394_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994713.1"
                     /translation="MSGIVVDLKRTGFPVKIGQVELWFDTSQERLIEFFDIETEVNRR
                     LNEYEKQVIEANLDNEIEGKGVTKDVAQSALDLEAKYLEINYDLLFGEGTFAQLYAEY
                     PDKEALENTLEIVCREIEVKLKELAIEREKIVKQKTKKYKKE"
     gene            9786..10367
                     /locus_tag="VC394_001245"
     CDS             9786..10367
                     /locus_tag="VC394_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage Gp15 family protein"
                     /protein_id="HEP5994714.1"
                     /translation="MKLNDPLVESFEFRGEIYPIDLSFNKVLDVFDVIDDDFLNEAEK
                     CFLCLDILLDRTDLPFTYAVDLWVYIKTNFIDAERPEKPQLDIKGNPMPVVKEKEDNK
                     KVIDLSLDAEFIYASFRQAYQINLLKEQNRLSWIEFKALLNALPDDTVMQRIIAIRQW
                     EDDGEGSKKYRDNMRKLKAKYSLDDGEEEDDGS"
     gene            10357..13617
                     /locus_tag="VC394_001246"
     CDS             10357..13617
                     /locus_tag="VC394_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HEP5994715.1"
                     /translation="MAADGKVTILVDVDGKQVKVLNSELDKVAKHGDKGSSSLKKFAV
                     GAGVFKLASTAVDLVSQSLGKAITRFDTLEKYPRVMKAMGHSAEDVARSTDKLANGID
                     GLPTTLDEVVGTAQRLTSITKDINKSTNLTLALNNAFLASGASSEAASRGLEQYAQML
                     SAGKVDMQAWKTLQETMPYALQQTAEAFGFAGASAQKDFYEALKNGQITFDQFSNKLI
                     ELNDGVGGFAELAKENSKGIETSFNNIKNAIAKGVANSIKALDDLSKAATGKGIADHF
                     DSLKVVINASFSAINASIKASTPLFKLLFSVIGAGISVVKALSPALVGVASGLAAMRA
                     VNETITMIKALNRAWVMASASMSIGATTIKTVTAVQAVSTTMTKADMVARLSQLGVLK
                     ASTVIYGVMTGAISLSTAATIAGTAAVTALKAALVALTGPVGWVVGAIGALVAVGVSL
                     WSWLTKESDETKKLKKEQEGLVESNKQLRDSVREGVQERKKGLESVKESTAAHQKLAD
                     EIIKLAAKENKTAGEKRNLKNKIDELNGSIDGLNLAYDKNSNSLSHNADQIKSRISAM
                     EAESTWQTAQQNLLNIEQKRSEVSKKLAENAELRKKWNEEANVSDSVRKEKIAELTEE
                     EAKLKNMQTQLQEEYNKTSATQQAAADAMAAAEESGSARQVIAYENMSEAQRTAIDNM
                     RTKYSELLETTTSIFDAIEQKTALSVDQMNANLEKNRAATEQWATNLEILAQRGVDQG
                     ILEQLRRMGPEGATQTQVFVDATDAELAPLQENFRAATETAKNAMGSVLDSAGVEMPE
                     KVKGMVTNVSTGLQTELQAANFAQLGQEIPNGVSQGISQGAGKASDASVKMGQEVKRS
                     FQGELGIHSPSRVFTEYGGHITDGLSNGVTNGTSKVMQTMQSLAQQMSQKGQQIVNDM
                     RSKSNQITDAFSTMSGPMHSHGVNAMQGLANGIYAGSGAALAAAQSIAARITATIQSA
                     LDIHSPSRVMRDEVGRFIPQGIAVGIDADRKVIDSSMQKLKESMTINATPEIASGFGG
                     GVAGIANQITNNSNNSFTLNVKVDESDGNSREKYQRLFREFSWYIQQQQGRLGDVK"
     gene            13614..14330
                     /locus_tag="VC394_001247"
     CDS             13614..14330
                     /locus_tag="VC394_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HEP5994716.1"
                     /translation="MTAFIKFDGKKSSDFDLRIINDVEHDSSFYDVDQVKVPGRDGVV
                     LKDNKRLEAIERSYPLRLYSKRRLTEVETDISNWLNVKGWKDLELSWEPDYIYKATHI
                     TPFSIKEVLRNFGRLKINFLIHPIKYLKTGKQEVPLVNGGTLQNPGNVQAKPILKIKG
                     TGNGVLTINDFETGLENVQGELVIDMERHLVYKDVLSAWDNIVRTERHRMPLFDVGQN
                     KISWTGSFTITAVPNWGVKV"
     gene            14327..>15997
                     /locus_tag="VC394_001248"
     CDS             14327..>15997
                     /locus_tag="VC394_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEP5994717.1"
                     /translation="MIPVLYEAKETKFRTFGLGEIADAYEVKATRERNGNYSLYIKYP
                     LDGVFASVFKEEMKIKSDAGRRTKWQTFEINRVLRNSKDHIEIFARHISMRTQDIALK
                     PFVNGASVGAESALEIWKKNLVGDDKFDVKSDILTLGSFNWEIDKIGNARDALGGVAG
                     SILDVYGGEYEFDNRTIILRKQMGRKAPTVLEYGRNIVSVEEERLLDGNYTSIYPYVR
                     YTPQPKPQEETPGKPHVGEHKQPEEQLVTLPEFILDGQYLSLYAQRRIQMVDLSSHFN
                     DDKNKKEPTIEEIRKLAQKYLKDNNVGAPKVSIEVDYIDLSQTLDYQDFRVMEEVELC
                     DIVPLYYPKFGITTESEKVVEIVYDVYTDSNHTIKLGTIGQSISKSLTGGVFERINAL
                     ENNQKVITNNQKQFELNLPKYLNDINGKRVWYEKPDDNIEHKIGDYWFEKNGKYQRTW
                     IWDGHQWVKVLDTEDLNPNQRAFDEAMAEIEKVKKAQEEINQRTDKELDEFRETLKNL
                     ALPEEAIKKITETIKVDDIPSIKQSFDDLKNRVSETSEESRLTAEILGN"
BASE COUNT         5371 a         2793 c         3701 g         4132 t
ORIGIN      
        1 gctataattt caatcaaacc tcaatttgta gatgagataa tgaaaggtaa caagcgtttt
       61 gaatttagaa aatctttttt aaaaagtatt cctgatagat gttacattta ctctacaaaa
      121 cctgtaggca agatagttgg tttttttacc attaaacaag ttttaagaga tgaaccggaa
      181 aaaatatggc aaaaaacaag taaaaagtca gggattacaa aggatttttt tgacgaatat
      241 tattcaggaa aaaataaagc ggtagcaata gaaatagata ggttgtttgc ttacaaaaca
      301 cctaagaatt atcatgaaat agatataaaa gggaagccac cgcaaacata taaaacgatt
      361 tgaagaagct gattgcttct ttttttattt ttaaaagtaa ggaagtgagg cgattggcta
      421 atgaagaaaa cttaatacca ggaaataaac gaagtaagag tgaacttaga gaaatcacta
      481 gaaaaggcgg tatagcttcc ggtaaagcaa gaagacgcaa aaaagaatta aaaaccataa
      541 tcgaacaagc gctcaactct gttatcccaa atgaaaaagc acaaaaaaag ttagaaagtc
      601 taggttttga tccaacattt cagtcagcta tcgctttgaa agtggtcgaa caagcgatga
      661 atgggaattt aagagctgtt gaattaatat ctaatatatc ttttgcagga aaagatagcc
      721 tggatagaaa agaacaaaga cagcgaatta aggccgcgga gctcaccaca gacgagcaac
      781 gaacgcgcat cgagctgctt aaagtcaaac ttgacgcaga gaaaggcgct aagcctgata
      841 ctagcttaat gagagctttg ttagatgctg tggaaggagg tgattagcct tggagattat
      901 cttttcaaat aaacaaaaag acgtcattag acgccatttt aactacgaat tagaggtcaa
      961 cgagggcact tgacccctcg tagcggaaag accacagctg ggcattttcg ttatgctagg
     1021 tatctgattg agtcagaaga tgaaaatcac cttgtgactg cttataatca agaacaagct
     1081 tatcgtttgt ttatcgacgg cgatggtacg ggtttgatgc atatatttga cggtaactgc
     1141 gaaataaaac acgacgagcg tggagatcac ttgttaatca cgacaccaaa aggcaataag
     1201 cgcgtttatt ataaaggcgg cggtaaagtt aacagtgttg gtgctattac aggtatgtct
     1261 ttaggatcag tagtattctg cgagattaac ttactgcaca tggattttat ccaggagtgt
     1321 tttaggcgta cttgggcggc taagctacgt tatcatctag cagatttaaa tcccccagca
     1381 cctcaacatc cagtaattaa agatgtcttt gatgttcaga acacgaggtg gactcattgg
     1441 accatggatg ataacccaat actaaccgca gagcgtaaac aaaacattat caacagtctt
     1501 aaaaaaaatc catatctata caaacgagat gtacttggac agcgggtcat gcctcaggga
     1561 gttatttatg gcctttttga cacggaaaaa aatgttttgg atgctttgat tggcgaacca
     1621 gtagagatgt atttctgtgc agatggaggt caatcagatg ccacctctat gtcttgtaat
     1681 atcgtaacaa gagttagaga taacggtagg ataagcttca gacttaatcg tgtagctcac
     1741 tactaccaca gcggagctga cactggccaa gtaaaagcta tgtcaaccta cgctttagag
     1801 ttaaaagttt ttatagactg gtgcgttaaa aagtatcaga tgcgctatac agaggtattt
     1861 gtggatcctg cctgtaaatc tttgagagag gagctgcata agttaggagt atttactctg
     1921 ggagctccga acaattctaa agatgtatct agcaaagcaa aaggcattga ggtcggtatc
     1981 gaacgcggcc aaaacattat ctcagatggc gctttttatc tcgttaatca tagtgaggaa
     2041 gagtatgacc attaccactt tttaaaagag atagggctat atagtcgtga tgacaatggc
     2101 aaacctattg ataaagataa ccatgccatg gacgagttta gatacagcgt caacgtgttt
     2161 gtgcatcggt attacaacta aaggagttgc ttacatggga gtaatccaaa aaataaaaaa
     2221 tcttgttaca aggagtaaat acgtgatgac aacgcagagt cttacaaata taaccgatca
     2281 tcctaaaata gctatcagta agctagagta cgatcgtata acaaccaatc taaagtatta
     2341 taagagcgat tgggatagtg ttttatactt aaacacggac ggcgagacta aaaaaagaga
     2401 tcttaaccat ctaccaattg cacggacagc tgctaaaaag attgccagtc tagtctttaa
     2461 cgagcaggca gagattaagg ttgatgatga tgcggctaac gaatttatta gtgagacact
     2521 aaaaaacgac aggtttaata aaaacttcga gcggtatctg gagagttgtt tagcgttagg
     2581 cggattagct atgaggcctt atgtggatgg tgataaagtt agggtagcat ttgttcaagc
     2641 gcctgttttt ttgccactgc agagtaatac gcaagacgtt tcgagtgccg ccgtcgtcat
     2701 taagtccgtt aagacaatca acggcaaaga ggtctactat accttgatag agttccatga
     2761 gtggcagagc tctgatgatt atgttatctc aaatgagtta tatcgctcgg atgataaagc
     2821 caaagtaggt agccgagtgc cgttatctga ggtatacaag gacttaaaag acgaggcaaa
     2881 agttacagat gtgactaggc ctatctttac ttatctcaag actcctggaa tgaataacaa
     2941 ggatattaat agcccacttg ggctatctat ctttgataac gccaagacaa cgattgactt
     3001 tatcaatacg acctatgacg aatttatgtg ggaagttaag atgggtcaac gtcgagttgc
     3061 tgtgccagag agcttgactg ctttaactgt tcgtaccact gatggcgatg ttgttccaag
     3121 gcctcgcttc gagtctgatc aaaacgttta tatccgtatg ggtggcaggg atttagactc
     3181 aagcgcaata caggacctaa caacacctat cagagcggat gactatatca aggctatcaa
     3241 cgaggggttg tctttgtttg agatgcaaat aggtgtatct gctggattat ttagttttga
     3301 tggcaaaagc atgaagactg caacagaaat cgtctctgaa aactcagaca cctaccaaat
     3361 gcgtaacagt attgttgctt tagtagagca atcgctaaaa gagttagtta tctctatttt
     3421 tgagattgct aaagcttatg atttgtacca aagcgaagtt ccaagcatgg ataacatcag
     3481 cataagtctt gatgacggtg tttttacaga tcgagacgct gagttagact actggataaa
     3541 agttgttaat gctggctttg gcactcgtga gatggccatc caaaaagtgc taaacgtgac
     3601 agaggaaaaa gcccaagaaa tagctgcaga aattaatact ggaatcgttg acgaaatcaa
     3661 tcaacagcgc actgatacac atttatacgg agagtgatta gatgaaaaag aagcctatta
     3721 agttaaatga cgaacagctt cttttggaag ctagtcagtt atctgatatg tatcatcagc
     3781 tgactcttga tttatttgat caagtgattg agaggataaa agccagaggc tcagcgagct
     3841 tagccgataa cccttatctt tggcaagcta ataagttaca tgacgttgga ctgcttaatg
     3901 cagataacat caagcttatt gcaaagtatt ctggcattgc ggaagctcaa cttcgctata
     3961 ttatcaagaa tgaaggattt aaaatttata aaaacacgtc tgagcagcta gaagaggctc
     4021 taggtagaga gtctggggta aacagtacta tccaagacga cctatctaac tatgctagac
     4081 aagctattga tgatgtgcat aatttgacta acaccacctt gccatttagt gttataggag
     4141 cttatcaagg gataatccaa gacgctgttg ctggtgtggt gacaggctta aaaacgcctg
     4201 accaagctat caatcaaact gtgattaaat ggtttaaaaa ggggttttat ggttttacag
     4261 ataaagctgg gagaaagtgg agagcagact cttatgctcg taccgttatc aatactacga
     4321 cttggcgagt ctttaacgaa gtcaaagaag cccctgctag ggagtttggc attgatacct
     4381 tctattactc aaaaaaagct acagctagag agatgtgtgc acctttgcaa catcaaattg
     4441 tcactactgg cgaagcgaga gaagaaggag ggataaaaat cttagcttta tctgattacg
     4501 ggcatggtga gcctgatgga tgcttgggaa tcaactgcaa gcacactaaa acgccgtttg
     4561 tcgtcggtgt gaatagtaag ccagaattgc cagagcatct aaaaaatatc actcctgcac
     4621 aagctaaagc taatgcgaat gcgcaagcta agcagagggc aatcgagaga tcaatacgta
     4681 agagtaaaga gctactgcac gttgcgaagc aattgggtga taaagagttg attaggcaat
     4741 atcaatcgga tgttagaagt aaacaagatg cacttaatca tcttgtcaat agtaatgact
     4801 ttttgataga aagtaagtct aggtctaaaa tgtttgttac agacctgatg aagcgtgaaa
     4861 ttgtgatgaa aaaagggtta atcaatgata taataggctt acagacaagt gatggaatca
     4921 ctataaaaga aattagcgga cacctcctgg aaagaatcta tgagaggggt gtctctgaga
     4981 gtcacatcgc tactgcatta gctaacccta tttacattag acctgatgca gtagatggcg
     5041 gtaggaaagt ttcaaggcgc tatgtaggca cccatgttac cgtaaatatt aacccacata
     5101 caggcaagat tataactact tggaagactg gtgaaaggac aaggaggaag tatgacaatc
     5161 agagaaatgt tgataagtaa tcaaatatta aataatgatg atattgattt tatgttatct
     5221 gtcaatcaag atgtctctcc tttgtttgag cttgaagata atgacgataa ctgggatgta
     5281 ttagtggacg agttgcagca aacctctagt tacttaacgg gagatggcga aagtgtctcg
     5341 gatagaacta agaagtttga tgatctagtt gataaaatat cagcaattta atcttataag
     5401 catttagcta aaaactaggt gcttttctta tgcttaaaaa caggaggaag acatgaataa
     5461 acgtatcaaa aagaaacgta aattggaaac agcggttgtg ttattgattg cagaaaacgc
     5521 aagacaagca gaagcaatta aaaatcaaaa caaacaaatc atggagctaa aatcaatcgt
     5581 tcaacgaaac gctctggcaa caaacgaaga gttagcgact gttaaagctg ctactttaga
     5641 taatcaatca gttatcaagg caattggtga cacggttgac tatattaaga aaaactacaa
     5701 acggaagtgg gggaaataaa gtttaaccgt gtcgaattcg acccctttag aaatcaaagt
     5761 cgtagcaata cggcttttta ttatgccttt atccgcaggc gttaaagaac ggaaatcagc
     5821 gacctatcgc atttatagga gggaatgcac atggaaaatg tgacaaacga aaacgtcgac
     5881 caagagacta ctgacttgga aaataatcaa caagaagata aagcatttac acaagatgat
     5941 ctcaatcgag tgggaactaa agagcattct aaaggctaca acaaagcagt caaagactta
     6001 ggttttgatg atgtggagtc agccaaggat gcactaaaag ccttcaaaga gtggaaagag
     6061 tcgcaaaaaa gcgagtcaga aaaacagaca gaaatcttga atgctaaaga caaagagctt
     6121 gaggaagctc gggcaaacaa taaggctctt aacgcaaagc tagcagcaat gtctttaggt
     6181 gtgaacgctg aatctattga tgatgtgatt gcactatctg agcgtcttgt cactgacgag
     6241 acaagcatag aagatgcaat caaaacggtt ttgggtaagt atcctcactt tggccaaaca
     6301 aaggataaag ctcccaaaat cacagtggca ggcaacccgt ctgctgataa cggacaaggt
     6361 tcggtgtcta aggaagactt tgcaaagatg tcttaccaag aaaaactgga cctaaaacta
     6421 aaaaataaga atctttatga ccaattgaaa ggaaattaaa aaatggcagt aggaacaact
     6481 aaaatggcac aaatgctaga ccctgaggtt ctagcggata tgattgacgc agaggtaggg
     6541 aaagcgattc gctttgctcc tcttgctgaa gtagatacaa ctttagaagg acaaccaggt
     6601 acaactttaa cagtgcctaa atgggattac atcggtgacg cagaagatgt tgccgagggt
     6661 gaagctatcc caatgactca acttggcttc aaaaagacta ctatgaccat taaaaaagcc
     6721 ggaaaaggtg tagaaatcac tgacgaagct atcttatctg ggtatgggga tcctgtaggt
     6781 caggcagcga agcaaatcgt tgaggctatt gaccacaaag tcgatgcaga cgtgcttgac
     6841 gcgttaagta aatcgactca aactgtggaa gctactgcta cggttgatgg tgtatctaaa
     6901 gcgctagata tttttaatga cgaagatgac gcagagacag tcattgtcat gaatccggcg
     6961 gatgcctcta ccctacgttt agatgccgcg aaggagtggt taggtgctac cgaggttgga
     7021 gcaaatcgtg ttgtctctgg tgtttatggt gaagttttag gggtacaaat tgtgcgttct
     7081 cgcaaatgcc ctaaaggaac tgcctacatg gttcgcaaag gtgcactacg tatcatgctt
     7141 aaacgtaaca caatggttga aacagaccga gatatcacaa aagcgattaa tcaaatcgta
     7201 gctaataagc attatggtgt ttatctttat aaggcagaaa aagccgtcaa aattacactt
     7261 aaagatgccg caaaaaagta gctaaggcta aggctgccaa gagagaggaa gatgacgctc
     7321 tcgagtctta gccgatggag gtggttatta tcgctttttt aacgcaaaaa gaatttgaag
     7381 atttgggttt tgacgatgta gaagattttg aaaaaatgga aaaacgtgct agccacgctg
     7441 tcaatcttta ctgtcgtaat cgctatgatt acaaagattt aaaaaaagaa atagccctag
     7501 tgcaaaaggc tgtaaagcgg gcaatcgctt atcagatagc atatttaaat gactcaggag
     7561 taatgacagc agaggataaa caatcctttg ctggaatatc tctaggacgg acaagtatta
     7621 gctacactgt cgggcatggc caaggcagcc aacaaaaaac tctggcagac aggtttaatc
     7681 tctgtctaga tgcagagaat gagttactag ttgttgggtt gggatatacg ggtattagct
     7741 atgatcgata aaagactgct tattgacgaa ctgcaggtaa aacttgtcaa agataaaggt
     7801 gattatggag ggtttgtcta tgacgaacct tttacactct ctccagttag atttgaccgt
     7861 aatcttgcaa ccgcaggtaa agataatgca aggcaagaga ctaaaccgtc ggttatcttt
     7921 atttatccta aatactgtaa gacagtagcc gataggtcat gggttgatgc tgttgtcatt
     7981 gatggtgata ctgagtacac cgttgataaa gtgataccag tctaccaccc actaacaaac
     8041 aaaatttttt gttttgaagt ggaggttatc taatggctaa ggtagtggta gagttgggag
     8101 gcatcaagcg taaagtatct ccgcaagcat tagctaaagg aaagctcatc atgaacaacc
     8161 aagtcatgat gtccatgaac ccttatgttc cttacagaga cggagctttg agaggaagct
     8221 cgagagctaa cagtgtaggt gtaacgtgga gcggaccaca cgccagagcc cagttttatg
     8281 gtggtgctta caacaagtac aagtccttta aatttaaaaa gtacacaaca ccaggtacag
     8341 gcaaacgttg ggataaacgt gcgttagcta atgcaacaat tgtcaaggat tgggaaaaat
     8401 ccttactgag aggaatggga tttaaatgac aaatgacttt gcaactgttt tgaggcaatt
     8461 tgtcgaagga ttagacttag gcataaagcc tagacttgac tatctaacaa gacaagaaga
     8521 cctagccatt tatccaatgc caggaggtaa ggtaaataac gagtacatgg acggtactcg
     8581 tgagataagc ctgccttttg agattgcaat caaaactaaa aatcaggagt tagcaagcac
     8641 tgtgatgtgg actattaaca gtgctttgtc taactttgac ttaaaattac caagtcttaa
     8701 tcattcgtac acatttatta gccttgatgt cgaaaagccg tttttaaatg acttaagcga
     8761 tcaaggtttt tatatttatg tgctggatat tacagcacac ttagaaatag aagggaataa
     8821 ctaatgagac aaaagaacgc cctacgtggg cattttatcg caccttatgt aaaaggtgag
     8881 gaaaagacag aagttacaaa agagaagctt ttggaattgg ctagatggat taaagacatc
     8941 tcagacgata ccgacgaaaa gacagaagat gaggcgtact atgacggaga tggaacagaa
     9001 gaaacaaccg ttgtaggagt taagggcgcg tacactttcg aagggacgta tgatccagaa
     9061 gacaaagctc aagctcacat tgcaagtctt aagtataagc taggagatga gcgaaaagtt
     9121 tggcatttga ttgtatcggc tgatgggaaa acccagtggc taggggttgc aacagtgaca
     9181 gaaattattg ctggttctgg tgctgccgcc gattttgaag cctttggttg caagattact
     9241 tataactctc tgccaaagga aagcaaggaa attataccta aaaaaaatga attgagcgta
     9301 gcgatgtgag acaagggtgg gctaaaccac ccttttaagg aggaattatg tctggaattg
     9361 tagttgacct aaagcgcaca gggtttccgg tcaaaattgg gcaggtagag ctttggtttg
     9421 atacaagcca agagcgattg attgagtttt ttgatattga gacagaagta aatcgtcgcc
     9481 tcaatgaata cgaaaaacag gttatcgaag ccaacttaga caacgaaatt gaaggtaaag
     9541 gggttacgaa agatgttgcc caaagtgctt tagatttgga agctaaatac ttagaaatca
     9601 attatgatct actattcgga gaaggtacat ttgcacagct ctacgcggag tatcccgaca
     9661 aagaagcact cgagaatact ctggaaattg tttgcaggga aatcgaggtc aaactcaaag
     9721 agttagctat cgaaagagaa aaaatcgtta agcagaaaac gaaaaagtac aaaaaggagt
     9781 agcctatgaa actaaacgat ccattagtag agtcgtttga gtttagaggt gaaatctatc
     9841 ctattgattt gagctttaac aaggttttag atgtctttga tgttattgat gatgattttt
     9901 taaacgaggc ggaaaagtgt tttttgtgcc ttgatatttt gctagatagg acagatttac
     9961 cttttactta cgcggtggat ctttgggttt atatcaaaac caattttatc gatgcagaaa
    10021 ggcccgagaa acctcagctc gacatcaaag ggaatccaat gcctgtagta aaagaaaaag
    10081 aggataacaa aaaagtcatt gatttgagtt tagacgctga gtttatctat gcgagcttta
    10141 ggcaagccta ccaaatcaat cttttaaaag agcaaaacag attgtcttgg attgaattca
    10201 aggctctttt aaatgctctt ccagacgata ctgtcatgca gcgtatcata gccattagac
    10261 agtgggaaga tgatggtgaa ggcagtaaaa aatacagaga taacatgcgt aagctaaagg
    10321 ctaagtacag tttagatgac ggagaggagg aagacgatgg cagctgatgg taaggtaacg
    10381 atacttgttg acgttgatgg taaacaggta aaggtgctca atagtgagtt agataaagtt
    10441 gccaagcacg gtgacaaagg cagctcctct cttaaaaaat ttgcggttgg tgcaggagtc
    10501 tttaaattag cttcgactgc agttgatctg gttagtcaat ctcttggcaa ggctatcaca
    10561 agatttgaca cgcttgaaaa atatccaagg gtgatgaaag caatgggcca tagcgctgag
    10621 gatgttgcga gatcaacaga taagctggct aatgggattg atggtttgcc aacaactcta
    10681 gacgaggttg tcggaaccgc tcaacgtttg acttctatca ctaaagatat caataagtcg
    10741 accaatctca cactagcttt aaacaatgcc tttttagctt caggagcttc atcagaggct
    10801 gcaagccgag ggctggagca atatgcccaa atgctatcag ctggtaaggt tgatatgcaa
    10861 gcttggaaaa ccctccaaga aacaatgcct tatgccttac aacaaactgc ggaagctttt
    10921 ggatttgcag gggcatcggc tcaaaaggat ttttatgagg cgttaaaaaa cgggcaaata
    10981 acatttgacc aattttctaa taagttgatt gagttaaatg atggtgtcgg cggttttgca
    11041 gaactagcca aagaaaatag taaagggatt gaaacctctt ttaacaacat caagaacgct
    11101 attgcaaaag gtgtggccaa tagcattaag gctttggatg atttgtctaa ggctgcaaca
    11161 ggtaagggca tagctgatca ttttgatagt ttgaaagttg ttatcaatgc ctcttttagc
    11221 gccatcaatg caagtattaa agctagtaca ccgctattta aacttttgtt tagtgttatt
    11281 ggtgctggaa tatcagtcgt caaagctctg tcgcctgcct tagttggtgt agcatctggt
    11341 ctagctgcca tgagggcagt taatgagact ataacaatga ttaaagcgct aaatagagct
    11401 tgggttatgg catctgcatc aatgagtatt ggagcaacaa ccattaagac tgtgactgcg
    11461 gtacaagcgg taagtaccac gatgactaaa gcagatatgg ttgcaagact atctcagtta
    11521 ggtgtcttaa aagccagtac cgtgatttat ggtgttatga caggcgctat cagtttatct
    11581 actgctgcaa ccatagccgg tactgctgcg gtaactgcgt taaaagcagc acttgtagcc
    11641 ttaacaggtc ccgttggttg ggtagtaggt gcgattggtg cgttagttgc tgttggagta
    11701 agtttgtggt catggctaac taaagagtca gatgagacta aaaagctgaa aaaagaacaa
    11761 gaagggctag ttgaaagtaa taaacagcta agagattctg tccgtgaggg cgtgcaagag
    11821 cgtaagaagg gccttgagtc cgtcaaagaa agcactgctg ctcatcaaaa attagctgat
    11881 gaaatcatta agttagcagc caaagaaaac aaaactgcag gcgaaaaacg aaatctgaag
    11941 aataagattg acgagcttaa tggatctatt gatggcttga acttggccta cgataaaaac
    12001 tcaaactctc tttcgcacaa cgcagatcaa attaagtcac gcattagcgc catggaagca
    12061 gaaagcacat ggcaaacagc acaacaaaac ctgttaaata ttgaacagaa acgtagtgag
    12121 gttagtaaaa agctagctga aaatgccgag ctacgtaaaa agtggaatga agaagctaac
    12181 gtctccgatt ccgtccgaaa agaaaagatt gcagaactca cagaagaaga agctaaactt
    12241 aaaaatatgc agactcaact gcaggaggag tataacaaga catcagctac tcaacaagct
    12301 gctgcagacg ctatggctgc cgctgaagaa tcaggatcag caagacaggt tatagcgtac
    12361 gaaaatatgt cagaagctca acgaactgcc atagacaata tgcgcactaa gtactctgaa
    12421 cttttagaga caacgacatc tatttttgat gctatcgaac aaaagacggc attatcagta
    12481 gatcaaatga atgccaacct tgaaaaaaat agagctgcta ctgaacagtg ggctactaat
    12541 ttggagattt tggctcagcg tggtgtggat caaggtattt tggagcaact aaggcgcatg
    12601 ggtcctgaag gagccacaca gacgcaagtt tttgtggatg caacagatgc cgagctagca
    12661 cccttgcagg aaaactttag agcagccaca gaaactgcta aaaatgcaat ggggagcgtt
    12721 ttagactcag caggtgtgga aatgccagaa aaagttaaag ggatggtcac taatgtttct
    12781 acgggattac agacggaact gcaagctgct aactttgctc aacttggcca agaaatccct
    12841 aatggagttt ctcaaggtat aagtcaaggg gcaggtaaag caagtgacgc aagtgtcaaa
    12901 atgggtcaag aagttaaacg ctcttttcaa ggagagttgg gtatccactc gccatcgcga
    12961 gtatttactg agtacggtgg ccatattact gatggcttga gtaatggtgt gacaaatgga
    13021 acgtcaaaag ttatgcaaac catgcagagc ttggctcaac agatgtctca aaaaggacag
    13081 cagattgtta atgacatgcg tagcaagtcg aaccaaatca cagatgcttt tagcacgatg
    13141 agtggtccaa tgcactctca tggtgttaat gccatgcaag gtttggccaa tggtatttat
    13201 gcagggtcgg gggcagcttt agcggcagct caaagcattg cggcacgtat caccgcaaca
    13261 attcaaagtg ccttagatat ccactcgcca tctcgtgtta tgagggatga ggttggacgt
    13321 tttatccctc agggtatcgc tgtaggtatt gatgcggata gaaaagtcat tgactcatct
    13381 atgcaaaagc taaaagagtc aatgacgatt aatgcgaccc cagaaatagc ctctggattt
    13441 ggcggaggag ttgcggggat tgctaatcag atcacaaata actcaaataa cagttttacc
    13501 cttaatgtca aggttgatga atccgacggt aatagccgcg agaaatatca acgcttattc
    13561 agagaattta gctggtatat tcaacaacaa caaggaaggt taggtgatgt taaatgacag
    13621 cttttatcaa gtttgatggt aaaaaatctt cagattttga tttgagaatt attaatgacg
    13681 ttgagcatga ctcgtccttt tacgatgttg atcaagttaa ggtacctggt cgtgatggtg
    13741 tggttttaaa ggacaataaa aggcttgagg ctattgaacg gtcttaccct ttacgtctat
    13801 atagcaaaag acgactcacc gaagtagaaa ctgacataag caattggctg aatgtaaaag
    13861 gttggaaaga cttagagttg tcatgggagc ctgattatat ctataaagca acacatatca
    13921 ccccttttag cataaaggag gttttaagga atttcggcag actgaaaatc aacttcttaa
    13981 tccaccctat caaatattta aaaacaggta agcaagaggt gcctctcgtt aatggcggta
    14041 ctctacaaaa tcccggcaat gttcaagcta aacctatcct aaaaatcaaa ggcacaggca
    14101 atggtgtttt aaccattaat gactttgaga cagggcttga aaatgtgcag ggcgagcttg
    14161 ttatagatat ggaaaggcac ctagtctata aagatgtcct atctgcttgg gataatatcg
    14221 taagaacaga acgccaccgc atgccgttat ttgacgttgg acaaaataaa atctcgtgga
    14281 ctggtagctt tacaattacc gcagtgccaa actggggggt taaagtatga taccagtttt
    14341 gtatgaggct aaggaaacca agtttaggac ttttggtctc ggtgagattg cggatgctta
    14401 tgaggttaaa gccactcgtg agcgcaatgg taattactca ctgtacatca aatatccgct
    14461 agatggtgtc tttgcctcag tttttaaaga ggaaatgaag attaagtctg acgctggtcg
    14521 tagaaccaaa tggcagactt ttgagattaa tcgggtacta cgaaatagta aagaccacat
    14581 cgagattttt gcgcgtcata tctctatgcg cacacaggat attgctttaa aaccgtttgt
    14641 aaacggtgcg agcgtaggag ccgaatcagc tttagaaatc tggaagaaaa accttgtcgg
    14701 tgatgataag tttgacgtta aaagcgacat cttaacgctt ggtagcttta actgggaaat
    14761 tgataaaatc ggcaatgccc gtgatgctct aggaggtgtc gctggctcta tcctagatgt
    14821 ttacggtggt gagtacgagt ttgacaaccg tacaatcatc ttacgcaagc aaatggggcg
    14881 taaagctccc acggtattgg agtatggccg taatatcgtc agcgtagagg aggagcgctt
    14941 gctagatggc aattacacct ctatctatcc ttacgtaaga tatacgccac aaccaaaacc
    15001 gcaagaggaa acccctggta agccgcatgt aggcgagcat aaacaacccg aagaacagct
    15061 ggtgacattg cctgaattta tcctagatgg tcagtatctc agcttatatg ctcagcgcag
    15121 aatccaaatg gttgatttat caagtcattt taacgatgac aaaaataaaa aagagccaac
    15181 gatcgaagaa atccgaaagc tggctcagaa ataccttaag gataataacg ttggtgcacc
    15241 aaaagtcagc attgaggttg attatattga cttgtcacaa acgcttgact atcaagattt
    15301 tagagtcatg gaagaggttg agctttgcga cattgtacca ctttattatc caaagtttgg
    15361 catcacaact gagtctgaaa aagtcgttga gattgtctat gacgtctata cagatagcaa
    15421 tcacacaatc aaattaggta cgattggtca atcaatctct aaaagtttga ctggtggtgt
    15481 ttttgaacgt attaatgcgt tggaaaataa tcagaaggta attactaaca accaaaaaca
    15541 atttgaactc aatctgccta aatacctcaa tgacatcaat ggtaaacgcg tttggtacga
    15601 aaaaccagat gacaatattg agcacaagat aggtgattac tggttcgaaa aaaatggtaa
    15661 gtatcagcgc acttggattt gggatggcca tcaatgggtc aaggtactag atacagagga
    15721 tttaaaccct aaccaacggg cctttgacga ggcaatggct gaaatcgaaa aagtcaaaaa
    15781 agcgcaagaa gaaatcaacc agcgcacgga caaagaactc gatgaatttc gtgaaactct
    15841 caaaaaccta gctttgccag aggaagccat caagaaaata accgaaacta tcaaagttga
    15901 tgacatcccg tctattaaac aaagctttga tgacctcaaa aatagagtga gtgagacaag
    15961 cgaagaatct cgtttaactg ccgaaatttt aggaaat
//
LOCUS       DATXQK010000035        15716 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.035, whole genome shotgun sequence.
ACCESSION   DATXQK010000035 DATXQK010000000
VERSION     DATXQK010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15716
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.035"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            65..241
                     /locus_tag="VC394_001249"
     CDS             65..241
                     /locus_tag="VC394_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEP5994718.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVTKAGTLQESRKREFYEKPSVK
                     RKRKSEAARKRKKF"
     gene            complement(369..731)
                     /gene="mscL"
                     /locus_tag="VC394_001250"
     CDS             complement(369..731)
                     /gene="mscL"
                     /locus_tag="VC394_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEP5994719.1"
                     /translation="MVKELKAFLFRGNIIELAVAVIIGGAFGAIVTSFVNDIITPLIL
                     NPALKAANVENITQLSWNGVKYGSFLGAVINFLIIGTSLFFVVKAAEKAMPKKEKEAA
                     APTQEELLTEIRDLLAQK"
     gene            861..2675
                     /gene="dnaG"
                     /locus_tag="VC394_001251"
     CDS             861..2675
                     /gene="dnaG"
                     /locus_tag="VC394_001251"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEP5994720.1"
                     /translation="MGFLWGGDDLAIDKEMISQVKNSVNIVDVIGEVVKLSRSGRHYL
                     GLCPFHKEKTPSFNVVEDRQFFHCFGCGKSGDVFKFIEEYRQVPFLESVQIIADKTGM
                     SLNIPPSQAVLASQHKHPNHALMTLHEDAAKFYHAVLMTTTIGQEARKYLYQRGLDDQ
                     LIEHFNIGLAPDESDYLYQALSKKYEEGQLVASGLFNLSDQSNTIYDAFRNRIMFPLS
                     DDRGHIIAFSGRIWTAADMEKRQAKYKNSRGTVLFNKSYELYHLDKARPVIAKTHEVF
                     LMEGFMDVIAAYRSGYENAVASMGTALTQEHVNHLKQVTKKVVLIYDGDDAGQHAIAK
                     SLELLKDFVVEIVRIPNKMDPDEFVQRHSPEAFADLLKQSRISSVEFFIDYLKPTNVD
                     NLQSQIVYVEKMAPLIAQSPSITAQHSYINKIADLLPNFDYFQVEQSVNALRIQDRQK
                     RQGQIAQAVSNLVTLPIPKSLTAIAKTESHLMHRLLHHDYLLNEFRHRDDFYFDTSTL
                     EILYQRLKQQGYITSYDLSEMSEEVNRAYYNVLEENLPKEVALGEIDDILSKRAKLLA
                     ERDLHKQGKKVRESSNKGDHQAALEVLEHFIAQKRKME"
     gene            2684..3793
                     /gene="rpoD"
                     /locus_tag="VC394_001252"
     CDS             2684..3793
                     /gene="rpoD"
                     /locus_tag="VC394_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEP5994721.1"
                     /translation="MTKQKEITTFNVQVAEFIRHHKKEGTAIDDDVTEKLVIPFALDA
                     DQIDDLLERLTDGGISITDKEGNPSSKYIVEEPKPEELTDEELIGSNSAKVNDPVRMY
                     LKEIGVVPLLTSEEEKELAVAVAKGDLMAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMEMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRVVLREQLDEVLDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRHPSRSKQLRDFIED"
     gene            4029..4367
                     /locus_tag="VC394_001253"
     CDS             4029..4367
                     /locus_tag="VC394_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEP5994722.1"
                     /translation="MSDTPKYTQDQVIAIKNRILEALETVIDPELGIDVVNLGLIYEI
                     RFNDNGYTEIDMTLTTMGCPLADLLTDYIHDALQDVPEVTKTEVKLVWYPAWTVDKMS
                     RYARIALGIR"
     gene            4505..5359
                     /gene="rfbD"
                     /locus_tag="VC394_001254"
     CDS             4505..5359
                     /gene="rfbD"
                     /locus_tag="VC394_001254"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEP5994723.1"
                     /translation="MILITGSNGQLGTELRYLLDERGVDYVAVDVAEMDITNEDKVEA
                     VFAQVKPTLVYHCAAYTAVDAAEDEGKALNEAINVTGSENIAKACGKYGATLVYISTD
                     YVFDGNKPVGQEWVETDRPDPKTEYGRTKRLGELAVERYAEHFYIIRTAWVFGNYGKN
                     FVFAMEQLAENHSRLTVVNDQHGRPTWTRTLAEFMCYLTENQKAFGYYHLSNDAKEDT
                     TWYDFAKEILKDKAVEVVPVDSSAFPAKAKRPLNSTMNLDKAKATGFVIPTWQEALKA
                     FYQQGLKK"
     gene            5478..6632
                     /locus_tag="VC394_001255"
     CDS             5478..6632
                     /locus_tag="VC394_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HEP5994724.1"
                     /translation="MQDVFIIGSRGLPAKYGGFETFVEELISHQSSKNIRYHVACLSD
                     TKHKVHFDYKGADCFYLNPPKLGPARVIAYDMMAITYALSYSDQHQIQNPIFYVLGNT
                     VGAFIAPFVKQIHNRGGRFFINPDGLEWKRSKWSKPVQAYLKFSEKQMTRQADLVISD
                     NIGIDRYLKQVYPWSKTCFIAYGTQTQPSRLTTADSKVRAYFQTFDIREKDYYLILGR
                     FVPENNYETAIKEFMASSTKRDLVIICNHEDNAYFKQLLAETECDKDPRIKFVGTLYD
                     KELLAYIREQAYAYIHGHEVGGTNPGLLEALAHTNLNLVLGVDFNQSVAKSAALYWSK
                     QKGQLAELINQVDAGFDSDRLGKEAKAIIQEHYTWEKIVGEYEALFLNEH"
     gene            6622..7554
                     /locus_tag="VC394_001256"
     CDS             6622..7554
                     /locus_tag="VC394_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEP5994725.1"
                     /translation="MNINILLSTYNGERFLAEQIQSIQRQTVNDWTLLIRDDGSTDGT
                     QDIIRTFVKEDKRIQWINEGQTENLGVIKNFYTLLKHQKADVYFFSDQDDIWLDNKLE
                     VTLLEAQKHEMTAPLLVYTDLKVVTQHLAICHDSMIKTQSGHANTSLLQELTENTVTG
                     GTMMITHALAEEWTTCDGLLMHDWYLALLASATGKLVYLDIPTELYRQHDANVLGART
                     WSKRMKNWLTPHHLVNKYWWLITSSQKQAQLLLDLPLKPNDHELVTAYVSLLDMPFTK
                     RLATLKRYGFRKNRIFHTFIFRSLVVTLFGYRRK"
     gene            7557..8360
                     /locus_tag="VC394_001257"
     CDS             7557..8360
                     /locus_tag="VC394_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP5994726.1"
                     /translation="MNFLTKKNRILLREMVKTDFKLRYQGSAIGYLWSILKPLMMFTI
                     MYLVFIRFLRLGGNVPHFPVALLLANVIWSFFSEATSMGMVSIVSRGDLLRKLNFSKH
                     IIVFSAVLGALINFLINLVVVLIFALINGVTISGYAYLSLFLFIELVVLVLGIALLLS
                     NVFVYYRDLAQVWEVLLQAGMYATPIIYPITFVLDSHPLAAKLLMLNPVAQMIQDFRY
                     LLIDRANVTIWQMSTNWFYVVIPYLVPFVILFIGIFVFKKNADRFAEII"
     gene            8360..9565
                     /locus_tag="VC394_001258"
     CDS             8360..9565
                     /locus_tag="VC394_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5994727.1"
                     /translation="MINREIAVKVQHVSKTFKLPTEATKSFRTTLVNRLRGIKGFTEQ
                     HVLKDINFDVYKGDFFGIVGRNGSGKSTLLKIISQIYVPEKGQVTVDGKMVSFIELGV
                     GFNPELTGRENVYMNGAMLGFTKDEVDDMYNDIVDFAELHHFMNQKLKNYSSGMQVRL
                     AFSVAIKAQGDVLILDEVLAVGDEAFQRKCNDYFMERKDSGKTTILVTHDMGAVKKYC
                     NRAVLIEDGLVKAYGEPFDVANQYSVDNTETAEDAMNAEKISVSDIAKDLKVSLISNP
                     RITPNDTITFEVSYEVLKDDFTYIAFSLTDIDRNIWVYNDNSLDYPTQGIGRKCISYQ
                     CQLSQLNDIKLKLEVTVRDKNGQMLLFSTAEQSPQIVIQRNDIDQDDLSALDSASGLY
                     QRNGKWVFH"
     gene            9590..10597
                     /locus_tag="VC394_001259"
     CDS             9590..10597
                     /locus_tag="VC394_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEP5994728.1"
                     /translation="MYKVSIICTNYNKAPWISDALDSFLSQVTDFEVEIIVIDDASTD
                     DSREILKSYQKKSSGKIKLLFNETNIGITKTWIKACLYAKGKYIARCDGDDYWTDSFK
                     LQKQVDVLEASKRSRWCNTDFDFVNSQGDLLYANAFSSGHTPLTDTYEKVLALKGMTM
                     ASTWLVDADLMRQVNQKINVDTPDDTFDIQLELFQLTQLTYIKDSTTVYRMTTDSDSR
                     PTDTPKMIHRIQKLLDTQLNYLKKYNQVDTKEMSELLLQQDAKQEIRIHELSCFIQEL
                     QQTIVDKTKQQETREVELQNVIEEQKNQLSELRQQYHAIINSRQWKYTSKLIAFIRRK
                     K"
     gene            10594..12339
                     /locus_tag="VC394_001260"
     CDS             10594..12339
                     /locus_tag="VC394_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-Rha alpha-1,3-L-rhamnosyltransferase"
                     /protein_id="HEP5994729.1"
                     /translation="MNRILLYVHFNKYNKISAHVYYQLEQMRSLFSKIVFISNSKVSH
                     EDLKRLKNHCLIDEFLQRKNKGFDFSAWHDGLITMGFDKLEEFDSLTIMNDTCFGPIW
                     EMGPYFENFEEKEAVDFWGITNNRGTKAFKEHVQSYFMTFKNQVIQNKVFQQFWQSII
                     EYENVQEVIQHYETQLTSILLNEGFSYQTVFDTRKAESSFMPHPDFSYYNPTAILKHH
                     VPFIKVKAIDANQHIAPYLLNLIRETTNYPIDLIVSHMSQISLPDAKYLLSQKYLNCQ
                     RLAKQTCQKVAVHLHVFYVDLLDEFLTAFEDWNFHYDLFITTDSDIKRKEIKEILQRK
                     GKTADIRVTGNRGRDIYPMLLLKDKLSQYDYIGHFHTKKSKEADFWAGESWRKELIDM
                     LVKPADSILSVFETDDIGIIIADIPSFFRFNKIVNAWNEHLIAQEMMSLWRKMDVKKQ
                     IDFQAMDTFVMSYGTFVWFKYDALKSLFDLELTQNDIPSEPLPQNSILHAIERLFVYI
                     AWGNSYDFRIVKNPYELTPFIDNKLLNLREDEGAHTYVNFNQMGGIKGALKYIIVGPA
                     KAMKYIFLRLMEKLK"
     gene            12336..14810
                     /locus_tag="VC394_001261"
     CDS             12336..14810
                     /locus_tag="VC394_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEP5994730.1"
                     /translation="MIKDTFLKTNWLNISHHIILLVFGFYFSFYSLAKELVSSTAQPV
                     NYYAHLLNVSFVGYIISLIGLSYYLSRQVSRQLFLKTSFIVISYLIVSYWVQITQHLN
                     DKRFDIWSLTKNQFYQFQALPSLLIILVMATLIKILVAYFAIEKDRFGLLGYQGNTFS
                     VALILAVVPINDIHLLKLISSRFSELVRAGNSQIALLKISGLLIVLLVIFATIIYVVL
                     NALKHLKSNKPSFSVAATTSLFLALVFNYTFQYGVKGDEALLGYYVFPGATLFQIVAI
                     TLVALLAYVITNRYWPTTFFLLILGTIISVVNDLKESMRSEPLLVTDFVWLQELGLVT
                     SFVKKSVIVEMVVGLAICIVVAWYLHGRVLAGKLFMSPVKRASAVLGLFIVSCSMLIP
                     FSYEKEGKILSGLPIISALNNDNDINWLGFSTNARYKSLAYVWTRQVTKKIMEKPTNY
                     SQETIASIAQKYQKLAEDINKDRKNNIADQTVIYLLSESLSDPDRVSNVTVSHDVLPN
                     IKAIKNSTTAGLMQSDSYGGGTANMEFQTLTSLPFYNFSSSVSVLYSEVFPKMAKPHT
                     ISEFYQGKNRIAMHPASANNFNRKTVYSNLGFSKFLALSGSKDKFKNIENVGLLTSDK
                     TVYNNILSLINPSESQFFSVITMQNHIPWSSDYPEEIVAEGKNFTEEENHNLTSYARL
                     LSFTDKETRAFLEKLTQINKPITVVFYGDHLPGLYPDSAFNKHIENKYLTDYFIWSNG
                     TNEKKNHPLINSSDFTAALFEHTDSKVSPYYALLTEVLNKASVDKSPDSPEVKAIQND
                     LKNIQYDVTIGKGYLLKHKTFFKISR"
     gene            14989..15684
                     /locus_tag="VC394_001262"
     CDS             14989..15684
                     /locus_tag="VC394_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEP5994731.1"
                     /translation="MKKLIIIPAYNESSNIVNTIRTIESDAPDFDYIIIDDCSTDNTL
                     AICQKQGFNVISLPINLGIGGAVQTGYRYAQRCGYDVAVQVDGDGQHNPCYLEKMVEV
                     LVQSSVNMVIGSRFITKEGFQSSFARRIGIKYFTWLIALLTGKKITDATSGLRLIDRS
                     LIERFANHYPDDYPEPETVVDVLVSHFKVKEIPVVMNERQGGVSSISLTKSVYYMIKV
                     TLAILVVRLKGNR"
BASE COUNT         5218 a         2548 c         3018 g         4932 t
ORIGIN      
        1 taaaaaataa tgtaatataa ttgggaacgt gaaaatgtta caatatcttg aggaggtgaa
       61 acacatgtca aaaacagtag tacgtaagaa tgaatctctt gacgatgctc ttcgtcgctt
      121 caaacgttct gttactaaag ctggtactct tcaagaatca cgtaaacgtg aattctacga
      181 aaaaccttct gtaaaacgta aacgtaaatc agaagcagct cgcaaacgta aaaaattcta
      241 atggtaaaaa ataaaaacag ttctcacaat tagaactgtt tttatttttt atccagaaga
      301 agcaagggag cttttttgat atcaaaaaaa gcagttagac atgcctaact actttatcaa
      361 aaatcgcttt atttttgagc caacaaatca cggatttctg tcaataattc ttcttgagta
      421 ggagctgcag cttctttttc ttttttaggc atagcttttt cagcggcttt tacaacaaag
      481 aaaagactgg taccgatgat aagaaaatta ataacggctc ctaaaaagct accatattta
      541 acaccattcc atgacaattg agtgatgttt tcaacatttg cggccttcag agctgggttt
      601 aaaataagcg gtgtgataat atcgttcaca aaagaagtga caatagcacc aaaagctccc
      661 ccgatgataa ctgctacagc aagttcaata atgtttcctc taaacaaaaa tgcttttaat
      721 tctttaacca ttaaatattc ccccttttaa tatctctaag ttaagcatag cataattagt
      781 aaacaaaatc caatggaatc acttcagttt ttaaatttac aaaaaagagt ttcggtggta
      841 taataaaacg taaaactaga atgggatttt tatggggagg tgacgatttg gcaattgaca
      901 aagaaatgat ttcccaagta aaaaatagcg ttaatattgt cgatgtcatt ggagaagtgg
      961 tcaaactttc ccgatcaggg cggcattatc tcgggctttg cccatttcat aaggaaaaga
     1021 caccctcttt caatgttgtt gaagacagac aattttttca ctgctttggc tgtggaaaat
     1081 caggggatgt ttttaaattt attgaggaat accgccaagt ccccttctta gaaagtgttc
     1141 agattattgc cgataagact ggtatgtcgc ttaatatacc gccaagtcag gcagtacttg
     1201 ctagccaaca caagcaccct aatcacgctt tgatgacact tcatgaggat gctgctaaat
     1261 tttaccatgc agttttgatg accactacca ttggtcaaga agctaggaag tacctttacc
     1321 agagaggctt ggatgaccaa ttaattgagc atttcaatat tggtttagcc ccagatgagt
     1381 cagattatct ttatcaagct ctttctaaaa aatacgagga aggtcaattg gttgcttcag
     1441 gattgtttaa cttgtccgat caatccaata ccatttacga cgcctttcga aatcgtatca
     1501 tgtttccctt atcagatgac cgagggcata ttattgcctt ttcaggacgt atctggacgg
     1561 cagctgatat ggaaaagaga caggccaagt ataaaaattc aagaggaaca gttcttttta
     1621 acaaatctta tgaattgtat catctggaca aagcaaggcc tgttattgcc aaaacccatg
     1681 aagtgtttct aatggaaggg tttatggacg tgattgccgc ttaccgttcc ggctatgaaa
     1741 atgctgttgc ttcaatgggg acggcattga ctcaagaaca tgtcaatcac cttaagcaag
     1801 tcactaaaaa agttgttttg atttatgatg gtgacgatgc tggacaacat gctattgcaa
     1861 aatcactaga attgcttaaa gattttgttg tcgaaattgt cagaatcccc aataaaatgg
     1921 atcctgacga atttgtacaa cggcattccc cagaagcatt tgcagatttg cttaagcagt
     1981 cacggatcag tagtgttgaa ttttttattg attacctaaa acctactaat gtagacaatc
     2041 tgcaatcaca aattgtttat gtggagaaaa tggcaccatt gattgctcaa tcaccatcca
     2101 tcacagctca acattcgtat attaacaaga ttgctgattt gttgccaaac tttgactatt
     2161 ttcaagtaga acaatcagta aatgcattaa ggattcaaga taggcaaaag cgtcaaggtc
     2221 aaatagctca agccgtcagc aatcttgtga ccttaccaat accaaaaagt ttgacagcta
     2281 ttgctaagac agaaagtcat ctcatgcatc ggctcttaca tcatgactat ttattaaatg
     2341 aatttcgaca tcgtgatgat ttttattttg atacctctac cttagaaata ctttatcaac
     2401 ggctgaagca acaaggatac attacatctt atgatttgtc agagatgtca gaggaagtta
     2461 accgtgctta ttacaatgtt ttagaagaaa accttcccaa agaagtagct cttggtgaga
     2521 ttgatgatat tttatccaaa cgtgccaaac ttttagcaga gcgcgatctt cacaaacaag
     2581 ggaaaaaagt tagagaatct agtaacaaag gcgatcatca agcggctcta gaagtactag
     2641 aacattttat tgcgcagaaa cgaaaaatgg aatagaggaa attatgacaa aacaaaaaga
     2701 aataacaact tttaacgttc aagttgcgga gtttattcgc catcacaaaa aagaaggaac
     2761 agcgattgat gatgacgtga ccgaaaaact tgtcattcca tttgctttgg atgctgatca
     2821 gattgatgat ttacttgagc ggttaacaga tggtggtatc tcgattacgg ataaagaagg
     2881 taacccatca tcaaaataca ttgttgaaga accaaaacca gaagaattaa ctgatgaaga
     2941 attaattggt agtaattcag ctaaagtaaa cgaccctgtc cgcatgtacc tcaaagaaat
     3001 tggtgtggta ccgcttttaa caagtgaaga ggaaaaagaa ttggctgttg cagttgccaa
     3061 gggagattta atggctaaac aacgtttagc ggaagcaaac cttcgtcttg ttgtctcaat
     3121 tgcaaaacgt tatgttggac gtggaatgca atttcttgat ttaattcaag aaggtaacat
     3181 gggactcatg aaagctgttg ataaatttga ttattcaaaa ggattcaaat tctcaactta
     3241 tgcaacatgg tggattcgtc aagctataac acgcgcaatt gcagatcaag cacgtacgat
     3301 tcgtatccct gttcatatgg ttgaaacgat taataaatta gtccgtgaac aacgtaacct
     3361 tcttcaagaa cttggtcaag atccaacgcc agaacaaatc gcagaacgta tggaaatgac
     3421 tccagacaag gttcgtgaaa ttctaaaaat tgctcaagaa cctgtttctt tagaaacacc
     3481 aattggggaa gaagatgata gtcacttagg tgattttatc gaagatgaag ttattgaaaa
     3541 tccagtagat tatacgacgc gtgttgttct tcgtgagcaa ttggatgaag ttttagatac
     3601 gcttactgac cgtgaagaga acgttcttcg cctacgcttt ggccttgacg atggtaaaat
     3661 gcgtacgtta gaggacgtag gtaaagtctt taatgttact cgtgagcgca ttcgacagat
     3721 tgaagctaaa gcccttagaa aacttcgcca tcctagtcgt agtaaacaat taagagactt
     3781 tatagaggac taaaatcgtc cagtggacga ttttaggtcg ttcctttgaa ataagaaagg
     3841 agcgaaaatc ctaagtggca cttatcagcg gtacacctac aaattagaaa tcggaccgaa
     3901 tgtggtgggt atttttaagg ttggacttga tatcttaaac aagcccagag ggcaattcct
     3961 ctaaataggc aaagctttag tggttttttt acttatctac gacatattca aaaaatgaaa
     4021 ggaaagccat gtctgataca ccaaaatata cacaagatca ggtgatagct atcaagaata
     4081 gaattcttga agctttagaa actgttattg atcccgagct aggtattgat gttgttaatc
     4141 taggtttgat ttatgaaatt cggttcaatg ataacggtta tacagagatt gatatgacac
     4201 tcacaacaat gggttgtcct ttggcagact tgttgacaga ttacatccat gatgccctgc
     4261 aagatgtgcc agaagtcact aagactgagg tcaaattagt ctggtaccct gcttggacgg
     4321 ttgataaaat gagccgctat gctaggattg cgctgggtat tcgctaatca ttgatagtaa
     4381 agggatgaaa tattcccttt tttgatgtag aataaaagga aatacaggtg aaagcctttg
     4441 gttaatgtta gagaacacta agatactgag aaaagtgttc tacctatggc aaaaaggaga
     4501 actgatgatt ttaattacag gaagcaatgg tcaattagga acagaacttc gatacttatt
     4561 agatgaacga ggtgttgatt atgtagccgt tgatgtcgca gaaatggaca tcacaaatga
     4621 agacaaagtc gaagctgttt ttgcgcaggt caagccaaca ttggtctatc attgtgctgc
     4681 ttatacggca gttgatgcag cagaagacga aggaaaagcc ttgaatgagg ctattaatgt
     4741 aacaggctca gaaaatattg ccaaggcttg tggaaaatat ggggctaccc ttgtttacat
     4801 ttcaacagac tacgtatttg atggcaataa accagtcggt caagaatggg tagaaacaga
     4861 tcgtccagac cctaagacag aatatggccg tacaaaacgt ttaggagaac tagctgttga
     4921 acgatacgca gagcattttt atattatccg tactgcttgg gtatttggaa attatggtaa
     4981 aaactttgtc tttgccatgg agcagttagc ggaaaatcac tcccgtttga ctgttgtcaa
     5041 tgaccaacac ggtcggccaa cgtggacaag aaccttggca gaattcatgt gttatttgac
     5101 agagaatcaa aaagcctttg gttactatca cttgtcaaat gatgctaagg aagacaccac
     5161 ttggtatgat tttgccaaag aaatcttaaa agacaaggct gttgaagtgg tgccagttga
     5221 ttcatcagct ttcccagcaa aagccaaacg tcctttaaat tcaaccatga atcttgataa
     5281 ggcaaaagca acagggtttg ttattccaac atggcaagaa gccttgaagg cattttacca
     5341 acaaggactg aaaaagtaag tattctaaaa agagttggct ttctaaggcc aactcttttt
     5401 catgggttta aggtcgcatt agccttctaa ttgtggtata atatcactga ttatatttta
     5461 gaatggggta gtatgcaatg caggatgttt ttatcattgg tagcagaggg ttaccagcga
     5521 aatacggtgg ctttgaaacc tttgtcgaag agttgattag tcatcaatca tctaaaaaca
     5581 ttcgttatca cgttgcttgt ttaagcgata cgaagcataa ggttcatttt gattataagg
     5641 gggcagattg cttttacctt aatcccccaa aacttggtcc tgctagagtt attgcttatg
     5701 atatgatggc aattacctat gccctgtctt atagcgacca acaccagatt cagaacccta
     5761 ttttttatgt gttgggcaat actgtaggcg cttttattgc gccatttgtg aagcagattc
     5821 acaatcgagg tggacgcttt tttatcaatc cagacgggtt agagtggaaa cggtctaaat
     5881 ggtcaaaacc agtgcaggcc tacctgaagt tttccgaaaa gcaaatgact agacaggctg
     5941 atttggtcat ttcagataat attggcattg accgctacct taaacaggtt tatccttggt
     6001 ctaaaacatg ttttattgct tatggaacgc agacacaacc ttcacgtttg acaacagcag
     6061 atagcaaggt cagagcttat tttcaaacct ttgatattag agaaaaggat tactatctca
     6121 ttttaggacg ctttgttcct gaaaataatt acgaaactgc tattaaagaa tttatggctt
     6181 cctcaactaa gcgtgattta gtgattattt gtaatcatga agacaatgct tactttaagc
     6241 aattacttgc tgagacagag tgtgataaag acccacgcat taaatttgtt ggtacccttt
     6301 atgacaaaga actattagcc tacattcgag aacaggctta tgcctatatc catggtcatg
     6361 aggtaggagg gactaatcca ggccttctag aagcactagc ccataccaat cttaatttgg
     6421 ttttaggagt tgattttaac cagtcggtgg ctaagtctgc tgcgctgtat tggtccaagc
     6481 aaaaagggca attagcagag ctgattaacc aagttgatgc aggttttgat agtgatcgtc
     6541 tgggcaaaga agccaaagct attattcaag aacattacac ttgggaaaaa attgtagggg
     6601 agtacgaggc tttattttta aatgaacatt aatattttac tatccaccta caatggagaa
     6661 cgcttcttag cagaacaaat tcaaagtatc caaaggcaaa cagttaacga ctggacctta
     6721 ttgattagag atgatggatc aactgatggc acccaggata ttattcgcac atttgtcaag
     6781 gaagacaaac gaattcagtg gatcaatgag gggcagacag aaaatctggg agttattaag
     6841 aatttttaca ccctcttaaa gcaccaaaaa gcagatgtct attttttcag tgaccaagat
     6901 gatatatggt tagacaataa actggaagtg accttattgg aggctcaaaa acatgaaatg
     6961 acagctcctt tattggttta tacagacctc aaagtggtaa cccaacattt agctatttgc
     7021 catgacagta tgattaagac ccaatctggt catgcaaaca caagcctact tcaagaatta
     7081 accgaaaata ccgtaacagg cggcacaatg atgattactc atgctctggc tgaggaatgg
     7141 accacttgtg acggtttatt gatgcatgac tggtatttag ctttactagc ttctgctact
     7201 gggaaattag tttaccttga tattccaaca gaattatacc gtcagcacga tgctaacgtg
     7261 ttgggagcta gaacttggtc taaacgcatg aaaaattggt taacccccca tcatttagtc
     7321 aacaaatatt ggtggttgat tacttcaagc caaaaacagg ctcaattact attagattta
     7381 ccattaaaac caaatgacca tgaattggta acggcctatg tttctttatt ggatatgcct
     7441 tttaccaaac gcttagctac cttgaaaagg tacggtttta gaaaaaatcg catctttcac
     7501 acctttattt ttcgtagttt agttgttacc ttatttggtt acaggagaaa gtaagaatga
     7561 attttttaac aaagaaaaac cgtattttac tgagagaaat ggtaaaaacg gattttaaat
     7621 tacgctatca agggagcgca attggctatc tatggtctat cttaaaaccg cttatgatgt
     7681 ttacgattat gtacttggta tttattcgtt ttcttcgcct gggtggaaat gtacctcatt
     7741 ttccagtagc gcttttattg gcaaatgtca tctggtcttt tttttcagaa gcaactagca
     7801 tgggaatggt atctattgta tctcggggag acttgttgcg aaaattgaac ttttctaagc
     7861 acatcattgt tttttcggca gtgttaggag ctttaattaa ttttcttatt aatttggttg
     7921 ttgttttaat ttttgcgttg ataaatggtg tgactatatc agggtatgct tatctctctc
     7981 tttttctttt tatagaatta gttgttttag tgcttggaat tgcattgtta ttgtcgaatg
     8041 tctttgttta ttatcgtgac ttagctcaag tctgggaagt actattacaa gcaggtatgt
     8101 atgccactcc aatcatttat ccgatcactt ttgttttaga tagccaccct ttggcggcaa
     8161 agttgttgat gctaaatcca gtagcacaaa tgattcaaga ttttcgttat ttattgattg
     8221 acagagccaa cgtaacgatt tggcagatgt caaccaattg gttttacgtt gttattccat
     8281 atttagtacc atttgttata ttatttattg gcatctttgt ctttaagaaa aatgccgata
     8341 gatttgcgga gattatttaa tgattaatag agaaattgca gtaaaagtac agcatgttag
     8401 taaaacattt aaactgccta cagaagcaac taaaagtttt cgcacgactc tggttaatcg
     8461 cctaagaggg attaaaggat ttactgaaca acatgtgcta aaagatatta actttgatgt
     8521 ttataaaggc gatttttttg gaattgttgg gcgtaacggc tcgggaaaat caacgctcct
     8581 taaaattatt tcccagatct atgttccaga aaaaggacag gttacagtag atgggaagat
     8641 ggtttctttt atagagcttg gagtgggatt caatccagaa ttaacaggac gagaaaacgt
     8701 ctatatgaac ggcgccatgc tgggatttac caaagacgaa gttgatgata tgtacaatga
     8761 tattgttgat tttgcagagt tgcatcattt tatgaatcaa aaattaaaaa attattcttc
     8821 tggcatgcag gtacgtttag cattttcagt ggctattaaa gcccaaggtg acgttcttat
     8881 tttagatgag gtgcttgctg taggagatga agcttttcag cgaaaatgta atgattattt
     8941 tatggaaaga aaagacagtg gtaagacgac tattttagtg acacatgata tgggagctgt
     9001 taagaagtat tgtaaccgtg cagttcttat tgaagatggt cttgttaaag catatggaga
     9061 accatttgat gttgctaatc agtatagtgt agataataca gagacagctg aggatgcaat
     9121 gaatgctgaa aaaatatctg tatctgatat tgccaaagat ttgaaagtgt cattaatctc
     9181 aaatccaaga ataacaccaa atgacacaat cacatttgaa gtttcttatg aagttttaaa
     9241 agatgatttt acctacattg ccttttcttt aacggatatt gatagaaaca tttgggttta
     9301 caacgacaat tctttagatt atccaacaca aggtataggt agaaaatgca tttcctacca
     9361 atgtcaacta tctcaactta atgatataaa actaaaatta gaagtaactg tccgagataa
     9421 gaacggtcag atgctactct tttcaacagc agaacaatca ccccagatag tgattcaaag
     9481 aaacgatatt gatcaagatg atctgtcagc tctagattca gcaagtggct tgtatcaacg
     9541 taatgggaag tgggtttttc attaatgagt aatagctaga agggaattta tgtataaagt
     9601 ttcaattatt tgcaccaatt ataacaaagc tccttggata tctgatgcac ttgacagttt
     9661 tttaagccaa gtgacagatt ttgaggttga aattattgtt attgatgatg cttctacaga
     9721 tgactcacga gagattttaa agagttatca aaaaaagtct tcaggaaaaa ttaaattact
     9781 ctttaacgaa actaatattg gaataacaaa gacttggata aaagcatgcc tttatgcaaa
     9841 aggcaaatat attgctagat gtgatggtga tgattactgg acggatagtt ttaaactcca
     9901 aaaacaagtt gatgtcttag aagcttccaa acggtcacga tggtgtaata ctgattttga
     9961 ctttgtaaat agtcaaggcg acttattata tgctaatgct ttttcatctg gtcatacacc
    10021 tttgacagat acttacgaaa aagttttagc cttaaaaggg atgacgatgg catcgacatg
    10081 gttagttgat gctgatttaa tgagacaagt aaaccaaaaa atcaatgtag atacaccaga
    10141 cgatactttt gacattcaac tagagttatt tcaactgact caactgacct atattaaaga
    10201 ctcaacgacg gtttatcgca tgacaaccga ttctgattca agacctactg atacaccaaa
    10261 aatgattcat cgtattcaaa aactacttga tactcagtta aattacttaa aaaagtataa
    10321 tcaggtagat acaaaagaaa tgtctgaact attattacaa caagatgcta agcaagaaat
    10381 acgaattcat gagttgagtt gctttattca agaattacaa caaactatcg tagataaaac
    10441 aaagcagcaa gaaacacgtg aagtggagtt gcaaaatgtc atagaagaac aaaaaaatca
    10501 actatctgaa ttaagacaac aataccatgc cattattaat tcacgtcaat ggaaatacac
    10561 gtctaagctc attgctttta ttaggagaaa aaaatgaatc gtattttatt atatgttcac
    10621 ttcaacaaat acaataaaat aagtgcacat gtttactatc aattggaaca aatgagatca
    10681 ctgttttcta agattgtttt tatctcaaat agtaaagtga gtcatgaaga tttaaaacgt
    10741 ctcaaaaacc actgtcttat tgatgagttt ttgcaacgca aaaataaagg ttttgatttt
    10801 tctgcatggc acgatggctt gattactatg ggatttgaca agttggaaga gtttgactca
    10861 ttgacgatta tgaacgatac ttgttttggt cctatttggg agatgggtcc ttattttgaa
    10921 aattttgaag aaaaagaggc agttgacttt tggggaatta ctaataatag aggtacaaaa
    10981 gcttttaaag aacatgtcca aagttatttt atgacgttta aaaatcaagt cattcaaaac
    11041 aaagtttttc aacaattttg gcaatcgata atagaatatg aaaacgttca agaagtgatt
    11101 cagcattatg aaacgcaatt aacatccatt ctcttaaatg aaggctttag ttatcagacg
    11161 gtctttgata ctagaaaagc tgagtcgtca ttcatgccac atccggattt ttcatattat
    11221 aatccaacag caattctaaa acaccatgtg ccttttataa aagtaaaagc tattgatgct
    11281 aaccagcata tcgctcctta cttactgaat ctgatacgag aaacaaccaa ttatcctata
    11341 gatcttattg tttcacatat gtcgcaaatc tctttgccag atgctaagta ccttttatct
    11401 caaaaatacc taaactgcca acggttagca aaacaaactt gtcaaaaggt agctgttcat
    11461 ttacatgttt tttacgttga tttgctagat gaatttttaa cagctttcga agactggaac
    11521 ttccattatg atctttttat tacaacagat agtgatatca aaagaaaaga gatcaaagaa
    11581 atacttcaaa gaaaaggtaa aactgctgat atacgcgtta cgggaaatag aggaagagat
    11641 atttatccaa tgttattact aaaagataaa ctttctcaat atgattatat tggtcatttc
    11701 catacgaaaa aatcaaaaga agctgatttt tgggcaggag aatcttggcg aaaagaattg
    11761 attgatatgc tcgtgaaacc agcagatagt attcttagcg tttttgagac agatgatatt
    11821 ggtattataa tcgcagatat tccaagtttt tttagattta ataagattgt caatgcttgg
    11881 aatgagcatt taatcgctca agaaatgatg tctttgtgga gaaaaatgga tgttaaaaaa
    11941 caaattgatt ttcaagctat ggatacattt gttatgagtt atggaacatt tgtatggttt
    12001 aaatacgatg ctctaaaatc actttttgat ttagaattaa ctcaaaatga tattccaagt
    12061 gaacctttac cacaaaactc tatattacat gcgattgagc ggttatttgt ctatatagcg
    12121 tgggggaatt cttatgattt taggattgtt aaaaatccct atgagttaac accttttatt
    12181 gacaataaac tgttaaattt acgtgaagat gaaggagctc atacctacgt taactttaat
    12241 caaatgggcg gtatcaaagg agcattaaaa tatattatcg ttggtcccgc taaagcaatg
    12301 aagtatatat tcttgcgttt aatggagaaa cttaaatgat taaagacaca tttttaaaaa
    12361 ccaattggtt aaatattagt caccatatta tccttcttgt tttcggtttt tatttcagtt
    12421 tttacagttt ggcgaaagaa ctagtaagct ccacggcaca accggtaaac tattatgctc
    12481 atttactaaa tgtttctttt gtgggatata ttatatcact gattggatta tcttattatt
    12541 tgagtcgcca agttagtcga cagttgtttt tgaaaactag ttttattgtg atatcttatc
    12601 taattgtcag ctattgggta caaataacac agcacctgaa tgataaacgg tttgatatct
    12661 ggtcattaac taaaaatcaa ttttatcaat ttcaagctct accttcttta ctcattattt
    12721 tagtgatggc cactttaata aaaatattgg tagcatattt tgcaatagaa aaagatagat
    12781 ttgggctatt aggctatcaa ggtaatactt tttctgtagc tctgatttta gcagttgtgc
    12841 caattaatga catacatctg ttaaaactaa taagttctcg attttctgaa ttagtaagag
    12901 caggtaatag ccaaattgca ctgttaaaaa taagtggact gttgatagtt ttacttgtca
    12961 tatttgcaac aatcatatac gtggttttaa atgctctaaa acaccttaag tcaaataaac
    13021 cttcattttc agtagcagct actactagtt tgtttttagc attagttttt aactatacgt
    13081 ttcagtatgg cgtaaaaggt gatgaagcat tgctaggata ttatgttttc cctggagcta
    13141 ctctttttca gatagtagct attacactag ttgctctttt agcatacgtg ataacgaata
    13201 gatattggcc aactaccttc tttttgctta ttctgggaac aattatttct gttgttaatg
    13261 atttaaaaga atcaatgaga agcgagccgt tattagtaac tgattttgtt tggttacaag
    13321 aattaggttt agtgacaagc tttgttaaaa aatcggtgat tgtagaaatg gttgtaggac
    13381 ttgctatttg tattgtggta gcttggtatc tacatggccg agttttagca gggaaattat
    13441 ttatgagccc tgtcaaacgg gcaagtgctg tattaggttt atttattgta tcttgtagta
    13501 tgttaatacc attttcttat gaaaaagaag gtaaaatatt atctggtctt ccgattattt
    13561 cggctttaaa taatgataat gatataaact ggttaggttt ttcaacaaat gctaggtaca
    13621 aatctttagc atatgtttgg acaagacagg tgaccaagaa aataatggaa aaaccgacaa
    13681 attatagcca agaaacaata gcgagtatcg ctcagaagta ccaaaaatta gcagaagata
    13741 ttaataaaga cagaaaaaat aatattgctg accaaacggt tatttatctt ttaagtgaaa
    13801 gtttgtcaga tcctgataga gtatcaaatg ttactgttag ccacgatgtt ttacctaata
    13861 tcaaggcaat caaaaatagc acaactgcgg gactcatgca gtcagactcc tacgggggtg
    13921 gaactgctaa catggagttt caaacgttaa caagcttacc tttttataat ttttcttctt
    13981 cagtatctgt tctttattca gaagtctttc ctaaaatggc caaacctcat acgattagtg
    14041 agttttacca aggaaaaaat cgtattgcga tgcatcctgc tagtgctaac aattttaata
    14101 gaaaaacagt ttatagtaat ttaggttttt ccaaattctt agctctatcg ggttctaagg
    14161 ataagtttaa gaacattgaa aatgtcggtt tattgactag cgataaaact gtctataata
    14221 atattttatc tttaattaat cctagtgaaa gccaattttt ctcagttatt acaatgcaaa
    14281 atcatattcc ttggtcatcc gattatcctg aagaaattgt tgctgaagga aaaaatttca
    14341 cggaagaaga aaatcacaac ctaacaagtt atgctcggtt attatcgttt actgataagg
    14401 aaacaagagc atttttagaa aaattaacac aaattaacaa gcctatcaca gtggtgtttt
    14461 acggagatca tttacccggt ttatatcctg atagtgcttt taacaagcat attgaaaata
    14521 aataccttac tgattatttt atttggagta atggtactaa cgagaaaaaa aatcatccgc
    14581 ttatcaactc aagtgatttt actgcagctt tatttgagca tactgattca aaagtatcac
    14641 cttactatgc tttgttaaca gaggtactga ataaagctag tgtcgataaa tcaccagata
    14701 gtcctgaagt taaagctatt cagaatgatt taaaaaatat ccaatacgat gtgactatag
    14761 gaaaaggtta ccttttgaaa cacaaaactt tttttaagat atcacgttaa gaaaaatgct
    14821 aggctgtccg agtatagacg agcatcaagg tttggtaatt tgctcaaatg attgtcaaca
    14881 gagtgtctag gaccaggttt aaaagattat aattcttaag gtcttattcg tttataaaaa
    14941 atgaatactc ttttaaaatc ttaattgaaa tgaataggag aagttttcgt gaaaaagtta
    15001 atcattattc ctgcttacaa tgaaagcagt aatattgtca atactatacg tactattgaa
    15061 tcagatgccc cggattttga ctatatcatt attgatgatt gctcaacgga taatacgtta
    15121 gcaatatgtc aaaaacaggg gttcaatgtt atttctttgc ccattaacct gggaattggc
    15181 ggtgcggtgc aaactggcta tcgttatgca caaagatgtg gatatgacgt tgcagttcaa
    15241 gtagatggag atggtcagca caatccatgc tatttggaaa aaatggttga ggtattagtt
    15301 caatcttcag taaatatggt aattggatca cgatttatca caaaagaagg gtttcagtca
    15361 tcatttgctc gacgtatcgg cataaagtat tttacttggc ttattgcgct actaacagga
    15421 aaaaaaataa cagatgcaac gtcaggtcta aggttaattg accgttcatt gattgaacgt
    15481 tttgctaatc attatccaga tgattatcct gaacctgaaa cagttgttga tgtattggtt
    15541 agtcatttta aagtgaaaga aattcctgtt gtaatgaatg agcgacaagg cggtgtgtca
    15601 tctatctcgt tgacgaaatc ggtctattat atgattaaag tgactttagc tattttagtt
    15661 gttagattga aaggaaatcg ttaaaatgac ggtaacagcc agtttattta taagtt
//
LOCUS       DATXQK010000036        15544 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.036, whole genome shotgun sequence.
ACCESSION   DATXQK010000036 DATXQK010000000
VERSION     DATXQK010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15544
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.036"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(71..376)
                     /locus_tag="VC394_001263"
     CDS             complement(71..376)
                     /locus_tag="VC394_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEP5994732.1"
                     /translation="MTHNHENDHQHEVITLVDEQGNETLFEILLTIDGREEFGKNYVL
                     LVPAGSEEDESGEIEIQAYSFTENEDGTEGDLQPIPEDSDAEWDMIEEVFNSFLDEN"
     gene            complement(388..807)
                     /gene="ruvX"
                     /locus_tag="VC394_001264"
     CDS             complement(388..807)
                     /gene="ruvX"
                     /locus_tag="VC394_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEP5994733.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIKIDEEKAEFGFTR
                     LEELVKQYQVEQFVIGLPKNMNNTNGPRVDASITYGNHIEHLFGLPVHYQDERLTTVE
                     AERMLIEQADISRGKRKKVIDKLAAQLILQNYLNRNF"
     gene            complement(804..1073)
                     /locus_tag="VC394_001265"
     CDS             complement(804..1073)
                     /locus_tag="VC394_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEP5994734.1"
                     /translation="MGFTDETVRFKLDDGDKRQISETLTAVYHSLDEKGYNPINQIVG
                     YVLSGDPAYVPRYNDARNQIRKYERDEIVEELVRYYLQGNGIDVK"
     gene            complement(1187..1585)
                     /gene="spx"
                     /locus_tag="VC394_001266"
     CDS             complement(1187..1585)
                     /gene="spx"
                     /locus_tag="VC394_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEP5994735.1"
                     /translation="MIKIYTISSCTSCKKAKTWLNAHKLAYKEQNLGKEPLTKEEILA
                     ILSKTENGVESIVSSKNRYAKALDCDIEELSVSEVIDLIQDNPRILKSPILIDDKRLQ
                     VGYKEDDIRAFLPRSIRNIENTEARLRAAL"
     gene            complement(1876..3012)
                     /gene="recA"
                     /locus_tag="VC394_001267"
     CDS             complement(1876..3012)
                     /gene="recA"
                     /locus_tag="VC394_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEP5994736.1"
                     /translation="MAKKLKKNEEITKKFGDERRKALDDALKNIEKDFGKGAVMRLGE
                     RAEQKVQVMSSGSLALDIALGAGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLSASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGTTQIKGTGDQKDSSIGKETKIKVVKNKVAPPFKVAE
                     VEIMYGEGISRTGELVKIASDLDIIQKAGAWFSYNGEKIGQGSENAKRYLADHPELFD
                     EIDRKVRVKFGLLEESEEESAMVVASEEIDDLALDLDNGIEIED"
     gene            complement(3101..4372)
                     /locus_tag="VC394_001268"
     CDS             complement(3101..4372)
                     /locus_tag="VC394_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEP5994737.1"
                     /translation="MKAELIAVGTEILTGQIVNTNAQFLSEKMAELGIDVYFQTAVGD
                     NEERLLSVITTASQRSDLVILCGGLGPTKDDLTKQTLAKYLRKDLVYDEQACQKLDDF
                     FAKRKPSSRTPNNERQAQVIEGSIPLPNKTGLAVGGFITVDGISYVVLPGPPSELKPM
                     VNEELVPLLSKQYSTLYSKVLRFFGIGESQLVTVLSDFIENQTDPTIAPYAKTGEVTL
                     RLSTKTENQALADKKLGQLEAQLLSRKTLEGQPLADVFYGYGEDNSLARETFELLVKY
                     DKTITAAESLTAGLFQSTLASFPGASQVFNGGFVTYSMEEKAKMLGLPLEELKSHGVV
                     SAYTAEGMAEQARLLTGADIGVSLTGVAGPDMLEEQPAGTVFIGLATQNKVESIKVLI
                     SGRSRLDVRYIATLHAFNMVRKTLLKLENLL"
     gene            complement(4441..5001)
                     /locus_tag="VC394_001269"
     CDS             complement(4441..5001)
                     /locus_tag="VC394_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEP5994738.1"
                     /translation="MKRCSWVPKDNQLYCDYHDLEWGQPLHDDRDFFELLCLESYQSG
                     LSWLTVLKKRQAFRTVFHHYDIASVAAFTSEEMADALENPSIIRHKLKLAATVNNAIA
                     VQKIQEEFGSFSTYLWNFVGGKPINNLVNQENLVPAQTELSIRLAKDLKKRGFKFLGP
                     TTVYSFMQASGLVNDHEEACVFNRIF"
     gene            complement(5011..5607)
                     /gene="ruvA"
                     /locus_tag="VC394_001270"
     CDS             complement(5011..5607)
                     /gene="ruvA"
                     /locus_tag="VC394_001270"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEP5994739.1"
                     /translation="MYDYIKGQLTKITAKYIVVETNGLGYIINVANPYSFTDSVNQLV
                     TIYLHQVIREDAHLLFGFHTEDEKDVFLKLISVSGIGPTTALAIVAVDDNEGLVNAID
                     NSDIKYLMKFPKIGKKTAQQMVLDLAGKFVEAPQETGNTKARSNKAGNTQLDEAIEAL
                     LALGYKAAELKKIRAFFEGTSETAEQYIKSALKLLMKG"
     gene            complement(5609..6829)
                     /locus_tag="VC394_001271"
     CDS             complement(5609..6829)
                     /locus_tag="VC394_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEP5994740.1"
                     /translation="MQEFLNLPKQIQLRQLVRFVTITLGSSIFPFMAMYYTTYFGTFW
                     TGLLMMITSLMGFVGTLYGGHLSDALGRKKVIMIGSVGTTLGWFLTILANLPNAAIPW
                     LTFVGILLVEIASNFYGPAYEAMLIDLTDESNRRFVYTINYWFINIAVMFGAGLSGLF
                     YDHHFLALLVALLLVNVLCFGVAYYYFDETRPETHAFDHGKGLLASFQNYRQVFHDRA
                     FVLFTLGAIFSGSIWMQMDNYVPVHLKLYFQPTAVLGFQVTSSKMLSLMVLTNTLLIV
                     LFMTVVNKLTEKWKLLPQLVVGSLLFTLGMLLAFTFTQFYAIWLSVVLLTFGEMINVP
                     ASQVLRADMMDHSQIGSYTGFVSMAQPLGAILASLLVSVSHFTGPLGVQCLFVVIALL
                     GIYFTVVSAKMKKV"
     gene            complement(6840..8822)
                     /gene="mutL"
                     /locus_tag="VC394_001272"
     CDS             complement(6840..8822)
                     /gene="mutL"
                     /locus_tag="VC394_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEP5994741.1"
                     /translation="MTNIIELPEVLANQIAAGEVVERPASVVKELVENAIDAKSSQIT
                     VEIEESGLKMIQVTDNGEGMSHEDLPLSLRRHATSKIKSQSDLFRIRTLGFRGEALPS
                     VASISKITIKTATKEVTHGSLLIATGGEIETLEAISTPTGTKIKVENLFYNTPARLKY
                     MKSLQAELAHIVDVVNRLSLAHPEVAFTLISDGRQLTQTSGTGDLRQAIAGIYGLNTT
                     KKMLAISNADLDFEVSGYVSLPELTRANRNYMTILVNGRYIKNFLLNRAILDGYGSKL
                     MVGRFPIVVIDIQIDPYLADVNVHPTKQEVRISKERELMALISTAISESLKEQDLIPD
                     ALENLAKSSTRHFSKPEQTQLPLQFRGLYYDPQKNDFFVKESAVSEKIPETDFYFGAV
                     DNSVKVEKAELLPHSEEVIGPSSVKHASRPQNTFIETDHPNLDLKNRQKLSQMLTRLE
                     NEEQSVFPELDYFGQMHGTYLFAQGKDGLFIIDQHAAQERVKYEYYRDKIGEVDSSLQ
                     QLLVPYLFEFSGSDFINLQEKMALLNEVGIFLEVYGHNTFILREHPIWMKEEEIASGV
                     YEMCDMLLLTNEVSIKTYRAELAIMMSCKRSIKANHSLDDYSARNLLLQLAQCQNPYN
                     CPHGRPVLINFSKADMEKMFRRIQENHTSLRELGKY"
     gene            complement(8917..10068)
                     /locus_tag="VC394_001273"
     CDS             complement(8917..10068)
                     /locus_tag="VC394_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEP5994742.1"
                     /translation="MIMKITEHKKKNGTIVYRASIYLGIDQMTGKRVKTSITGRTRKE
                     VNQKAKHAQFDFLSNGSTIKRKVVIKTFKELSHLWLETYKLTVKPQTYDATVTRLNRH
                     IMPTLGNMKVDKITASDIQMLINRLSKYYVNYTAVRSVIRKVLQQGVLLGLIDYNSAR
                     DIILPRKQPNAKKKVKFIDPSDLKSFLEHLETSQHKRYNLYFDAVLYQLLLSTGLRIG
                     EACALEWGDIDLENGTIAINKTYNKNLKFLSTAKTQSGNRVISVDKKTLRSLKLYQMR
                     QRQLFNEVGARVSEVVFATPTRKYFNASVRQSALDTRCKEAGIERFTFHAFRHTHASL
                     LLNAGISYKELQYRLGHANISMTLDTYGHLSKDKEKEAVLYYEKAMNNL"
     gene            10317..10460
                     /locus_tag="VC394_001274"
     CDS             10317..10460
                     /locus_tag="VC394_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HEP5994743.1"
                     /translation="MSGGGAYVCKSQKPKERRRQGLSVYETLTLMIAFGTLIVAIMNN
                     KNK"
     gene            complement(11322..12089)
                     /locus_tag="VC394_001275"
     CDS             complement(11322..12089)
                     /locus_tag="VC394_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEP5994744.1"
                     /translation="MAKNSPQDLINREIFSTNLNMLMAKKNIKQIDIHNKLGIPKSTI
                     TGYVKGRSLPTAGNVQKLADFFGVLKSDIDPRFDSNNIETNSNIIPSTLQKVTSTLSQ
                     LEHKRQLNVLDYAETQLEQQNTVEEPQATYYTYNYYDHAASAGTGQYLNDVQVETIEL
                     PVDYDADFVIPVYGDSMEPEYHSGDYVFIKLSINLSDGDIGVFEYYGDAYIKQLVIND
                     SGAFLHSLNDKYDDILIDRDSDFRIIGEVIGSFTSKS"
     gene            12243..12449
                     /locus_tag="VC394_001276"
     CDS             12243..12449
                     /locus_tag="VC394_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP5994745.1"
                     /translation="MTQITLKAARINAGYTLKQVAGAVGKNPQTISKYEKDSSDISLG
                     LLQKLSSLYGVTIDNLFLGKKVRK"
     gene            12483..12683
                     /locus_tag="VC394_001277"
     CDS             12483..12683
                     /locus_tag="VC394_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP5994746.1"
                     /translation="MTVGDKIKTIRLNLGETMEEFGKRFGTSKGTVNNWEKGRNLPNR
                     NSLLRIAQLANQTVSDFLAITD"
     gene            12704..13111
                     /locus_tag="VC394_001278"
     CDS             12704..13111
                     /locus_tag="VC394_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994747.1"
                     /translation="MARTPFTQELLHQIFDDTGTMSLELIAERLPDWSEKDIKLRLAA
                     WRYRNNIDYTMANGEIDTFEIINNRKAISEEVSAGRQLKLEEYFKQVQATAEIINKPT
                     ASDTNRLKAIQLQQVAMDEIPDQYFKELTELYG"
     gene            13523..14050
                     /locus_tag="VC394_001279"
     CDS             13523..14050
                     /locus_tag="VC394_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HEP5994748.1"
                     /translation="MTMRYLLEAFGVLSFEIHKPEKGSLGGRPRRIYHLNEQQATLLV
                     TYLGNTEPVREFKKNLVKAFFEMREELTQIRLQRSLEAPKRKTLNEAIKTWEHAPKMA
                     YPTVYNLLLKAVTGKNSKQLKVTRGGYSDIDCLNSIELAQYTALEDMAIALINLNFTY
                     QDIKTMALKNTLQCA"
     gene            14298..14471
                     /locus_tag="VC394_001280"
     CDS             14298..14471
                     /locus_tag="VC394_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000132665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEP5994749.1"
                     /translation="MTDTKKRVGRPKGENSNTVKLTVRVSEELDSKLNKYAKENNLTK
                     PETLRKAFENLTK"
     gene            14689..14862
                     /locus_tag="VC394_001281"
     CDS             14689..14862
                     /locus_tag="VC394_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEP5994750.1"
                     /translation="MSPKMGRPVKGTAKRDKRLEVRLTADEYNTIQETADKNGLSKAD
                     LIVKAVNSYESEK"
     gene            15003..15455
                     /locus_tag="VC394_001282"
     CDS             15003..15455
                     /locus_tag="VC394_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000916929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994751.1"
                     /translation="MLKNQDNMIKKKRNPRVLFRNVAYKLSEIEGKTLTEIASFLGFG
                     NSEVCRSTLYKWKRKKWLKFDLKNGHYRNVEVLHEVTLEKMANKELKEQGLIYKANIY
                     YEQVVSTSEIIEDIKTKTQDRIKAIHLQQKALERIPSELFAELYTNMN"
BASE COUNT         4998 a         3087 c         2708 g         4751 t
ORIGIN      
        1 ttatttattc gaaaataaca ttaaaaaaat agccctcacg gctacttttt aacatagtgt
       61 cactaacaat ttagttctca tctagaaaac tattaaatac ctcttcaatc atatcccatt
      121 cagcatctga atcctcagga atcggttgta aatccccttc agtaccatct tcattttcag
      181 taaatgaata ggcttggatt tcaatttcac cagactcatc ttcttcagat cccgctggaa
      241 ccaagagaac ataattttta ccaaactctt cacgaccgtc aattgttaat aaaatttcaa
      301 acaaggtttc atttccttgt tcatccacaa gtgtgataac ttcgtgttgg tggtcatttt
      361 catgattatg tgtcattttt tatctcctta aaaattccga tttaaatagt tctgtaaaat
      421 gagttgtgca gctaatttat caatgacttt ttttcgctta ccacgactaa tgtcagcttg
      481 ctcaataagc atacgctctg cctcaacagt cgtcaatcgt tcatcttgat aatggactgg
      541 aagtccaaag aggtgctcta tgtggtttcc ataagttata cttgcatcaa cacgtggacc
      601 attggtgtta ttcatatttt ttggcaatcc aataacaaac tgttcaactt ggtactgctt
      661 aacaagctct tctaaacgtg taaagccaaa ttctgctttt tcctcatcaa tcttgataat
      721 ttcaagtcct tgggctgtaa agccaagagg atcactaata gctacaccaa ccgttttcga
      781 gccaacatca agtcccatta ttctcattta acatcaattc catttccttg taaatagtag
      841 cgaacaagtt cttctacaat ttcatcacgt tcatatttac gaatctgatt tcgagcatca
      901 ttataacgag gaacataagc tgggtcccca ctcaagacat aaccaacaat ttgattaatt
      961 ggattataac ccttttcatc aagtgaatga tagactgctg ttaatgtttc actaatttgt
     1021 cgtttgtcgc catcatccaa cttaaagcgg actgtttcat ctgtaaatcc catacttaca
     1081 ccttctttct aaaattaagt gctatgcttt attatagctt atttctatta aataaacaag
     1141 aatttcaagc gaagaaggta tttaaaaaaa agctctacaa gagcttttag agtgcagcac
     1201 gtaatcgtgc ttcagtattt tcaatattac gaatagagcg aggtaaaaaa gcacgaatat
     1261 cgtcttcttt gtagcctact tgcagacgtt tatcatcaat caaaatggga cttttaagga
     1321 tgcgcgggtt atcttgaatc aaatcaataa cctcactaac acttagttct tcaatatcgc
     1381 aatcgagagc tttggcataa cgatttttag atgaaacaat gctctccact ccattttcgg
     1441 tttttgataa aattgctaaa atttcttctt tagttagcgg ttcttttcct aagttctgtt
     1501 ctttataagc aagcttatgg gcatttagcc aagttttggc tttcttgcag ctcgtacaac
     1561 ttgaaatcgt gtaaatttta atcatgttgc tgactcactt tctatttcgt gatagcttta
     1621 ttgtagcaca gtttcttatc tataaatata ggtctttgga cctatttttt taattttaaa
     1681 gatattatct tggtaaaata aggtttttca cctgtattcc cttgaattat tagttatttt
     1741 aattttatat cagttaacct aagttctgct ctctcacatc tttttaaatg atggtttcta
     1801 ttcacccaca cacctatgaa tagtagtcct actaaccttt aaaaatacat tttaaaaagc
     1861 cctgaagggc tctttttaat cttcaatttc aataccatta tctaaatcta aagcaagatc
     1921 atcgatttct tctgatgcta ctaccatagc agattcttct tcgctttctt caagcaaacc
     1981 aaatttaaca cgtactttac ggtcgatttc atcaaacaat tctggatgat cggccaaata
     2041 acgcttagca ttttcagaac cttggccaat cttctcacca ttataagaga accaagcacc
     2101 tgctttttgg ataatgtcca aatcagaagc aattttcaca agctcccctg tacgagaaat
     2161 accttcccca tacatgattt caacttctgc taccttaaat ggcggagcga ccttgttttt
     2221 aacaaccttg attttggtct ccttaccaat gctgctatct ttttggtcac cagttccttt
     2281 aatttgagtt gttccacgca catccaaccg aacagaagca tagaatttca aagctcgacc
     2341 acctggtgtt gtttcaggat ttccaaacat gacaccaact ttttcacgca attggttaat
     2401 aaagatagcg atagtttttg ttttattaat agaagctgat aatttacgca tagcctgact
     2461 catcatacgt gcttgcaatc cgacatggct atcgccaata tcaccatcaa tttcagcacg
     2521 tggcactaaa gctgctactg aatcgacaac aaccagatca accgcaccag aatcaatcaa
     2581 tttacctgca atttcaagtc cttgttctcc agaatctggt tgagacaaga ggagttcatc
     2641 aatattaacc ccaagcgcag cagcataagc tggatcaagc gcatgctcgg catcgataaa
     2701 ggctgcgatt ccaccttctt tttgagcttg tgctacagca tgtaaagcca cagtcgtttt
     2761 accggaagac tctggaccat agatttcgat gatacgtcct ttaggataac caccagctcc
     2821 aagcgcaata tcaagagcta gacttcctga actcataacc tgaacttttt gctctgcacg
     2881 ttctcctaat cgcatcactg cgcccttacc aaaatctttt tcaatatttt tcaaagcatc
     2941 atcaagggct ttgcgacgtt cgtcaccaaa cttcttagtg atttcttcat tcttttttaa
     3001 tttttttgcc aatactttct cctctttttt aacaaaccat cgtcagcagg attaatctac
     3061 ataatcgtaa accatatcct actatagcta ctaaataaca ttatagcaaa ttctcaagtt
     3121 ttaataaagt tttacggacc atattaaagg catgtaaagt agcgatatag cgcacatcca
     3181 aacgacttcg cccgctaatc aaaaccttta ttgattctac cttattttga gtggcaaggc
     3241 caatgaaaac tgtacctgca ggctgttcct ccaacatatc tggtccggca acacctgtta
     3301 aacttacccc aatatcagca ccagtcaata accttgcttg ctccgccatc ccctcggctg
     3361 tataagcact aacaacgcca tgcgatttca actcctctaa aggaaggcct agcattttcg
     3421 ctttttcttc catgctataa gtcacaaagc ctccattgaa tacttgagaa gctcctggaa
     3481 aactcgccaa agttgactga aataatcccg cggttagact ttctgctgct gtaattgtct
     3541 tatcatattt tactaagagc tcaaatgtct cacgcgctaa ggaattatcc tccccatagc
     3601 cataaaagac atcagctaag ggttgacctt caagagtttt tcgggatagt agctgcgctt
     3661 ctagctgacc taacttttta tctgccagag cttggttttc agtttttgtt gataagcgaa
     3721 gagtcacttc gccagtctta gcatacggag caatggttgg atcagtttga ttctcaataa
     3781 aatctgacaa gactgttacc aactgacttt ccccaatacc aaaaaagcgt agtacctttg
     3841 aatacaatgt actgtattgt tttgacagaa gtggtaccaa ttcttcattt accatcggct
     3901 tcaattcact tggaggaccc ggtaagacaa cataactaat accatcgact gtgatgaacc
     3961 caccaaccgc aagaccagtt ttatttggca aagggattga cccttcaatc acttgtgcct
     4021 gtcgctcatt atttggtgtc cgtgatgaag gcttgcgctt agcaaaaaag tcatctagtt
     4081 tctgacaagc ttgctcatca taaaccaagt ctttcctaag gtactttgct aaagtttgtt
     4141 tggttaaatc atctttcgtt ggaccaaggc caccacataa aattaccaag tcactacgct
     4201 gactagcagt tgtaatcact gaaagtaaac gctcctcgtt gtccccaaca gccgtttgaa
     4261 aatagacatc aatacctagc tctgccattt tttccgacag aaattgagca ttggtattca
     4321 caatttgacc agtcaaaatt tcggtaccta ctgcaatcag ttcagctttc atttccccac
     4381 ctacttatct attcgtaatc tgatctcatt ttatcaaaaa atcttccaat tcacgaagaa
     4441 ttagaagatt ctattgaaaa cacaagcctc ttcatggtca tttaccagac ccgatgcttg
     4501 cataaaagaa taaaccgttg taggccctaa aaatttaaat cctcttttct ttaaatcctt
     4561 agctaagcga atagataatt ctgtttgagc aggaacaaga ttctcttgat tgacaaggtt
     4621 gttaattggc tttcccccaa caaaattcca gagataagtt gaaaaactac caaactcttc
     4681 ttgaattttc tgtactgcga tagcattgtt gacagttgct gctagcttta gcttgtggcg
     4741 aatgatggat ggattctcta aagcgtcagc catttcctcc gaggtaaagg ctgcaaccga
     4801 tgcaatgtcg taatgatgaa aaacagttct gaaagcttgg cgttttttca aaacagtcag
     4861 ccaggataag cctgactggt aactttcaag gcagagtaat tcaaaaaaat ctctatcatc
     4921 atgtaggggt tgaccccatt ctaaatcgtg gtaatcacag taaagctggt tatccttagg
     4981 aacccatgaa caacgtttca tgtgaaacac ctagcctttc attaatagtt taagggctga
     5041 tttgatgtat tgttcggccg tttcagaggt tccttcaaag aaggcacgaa ttttcttcaa
     5101 ttccgctgcc ttataaccga gggcaaggag ggcttcaatc gcttcgtcca gttgagtatt
     5161 gcctgcttta ttgctgcgcg ctttggtatt accagtctct tgtggagcct ccacaaattt
     5221 gccagctaag tcaagaacca tttgctgcgc tgttttttta ccaattttag gaaatttcat
     5281 taggtactta atgtcactgt tatcaatggc attcacaagt ccctcattat catcaactgc
     5341 cacaatagca agagctgttg tggggccaat gccagataca gaaattaatt tcagaaaaac
     5401 atctttttcg tcttccgtat gaaacccaaa caacaagtga gcatcctcac gaatcacttg
     5461 atgcaggtaa atagttacca gttggttgac actatctgta aagctataag gattggctac
     5521 attgataata tagcctagtc catttgtttc aacgacaatg tattttgccg taattttggt
     5581 caattgacct ttaatataat cgtacatact acaccttttt catttttgca gaaacaaccg
     5641 taaaataaat ccctagcaaa gcaatgacta caaataagca ttgcacacct aaaggacctg
     5701 taaaatggct gacagatact agtagactag ccaaaatagc acctaggggt tgtgccattg
     5761 acacaaaacc tgtataagat cctatttggg aatgatccat catatcagca cgtaggactt
     5821 gactagcagg aacatttatc atttccccaa aagttaacaa aacaactgat agccaaatag
     5881 catagaactg cgtaaaggta aatgccaaga gcatccctag agtaaatagt aaagaaccaa
     5941 ccacaagctg aggtaatagt ttccattttt ccgttaattt atttactact gtcatgaaaa
     6001 ggacaatcag caatgtatta gttaaaacca ttaatgataa cattttagaa ctagttactt
     6061 ggaaacctaa cacagccgtt ggctgaaaat acagtttcaa atggactggc acatagttat
     6121 ccatctgcat ccaaatacta ccagaaaaga tggcacctaa ggtaaacaag acaaaggcac
     6181 gatcatgaaa cacctgacgg tagttctgaa aactcgccag taatccttta ccatggtcaa
     6241 aagcgtgtgt ttctggtcta gtctcatcaa aatagtagta agcaacgcca aaacaaagta
     6301 cattaacgag taataaggct actaacaagg ctaaaaaatg atggtcataa aaaagcccag
     6361 atagccctgc accaaacatg acggcaatat tgataaacca ataattgatg gtgtaaacaa
     6421 atcgacgatt actctcatca gtcaaatcaa ttaacatagc ttcataggca ggaccataaa
     6481 aattagaagc aatctctacc aataaaatac ccacaaaggt taaccaagga atagctgcat
     6541 taggcaaatt agctaaaata gtcagaaacc agcctagtgt tgttcctact gacccaatca
     6601 taatgacttt tttgcgacca agagcatctg acagatgccc accgtataaa gttccaacaa
     6661 atcccatcaa actggtaatc atcattaaga ggcctgtcca aaacgtacca aagtaagtcg
     6721 tataatacat ggccataaag ggaaaaatac tactgcctaa ggtaatggtc acaaagcgta
     6781 ccagttgcct cagctgaatc tgcttaggaa gatttaaaaa ctcttgcatc tcatcctctc
     6841 taatattttc ctagctctcg caggctagtg tgattttctt ggatacgacg gaacattttt
     6901 tccatatctg ccttactaaa attaatcaat acgggtctac catgagggca gttataagga
     6961 ttttgacatt gggccaattg cagtagcaga tttcttgctg aataatcatc caagctatga
     7021 tttgccttga tagatcgttt acaactcatc ataatagcta actctgctcg gtaagtttta
     7081 atagatacct cattggttag aagtaacatg tcacacattt catagacacc agatgcaatc
     7141 tcttcttctt tcatccagat aggatgctcc ctcaaaatga aagtattgtg cccataaact
     7201 tctaagaaga taccaacttc atttaagagt gccattttct cctgtaagtt aatgaaatca
     7261 gaaccagaaa actcaaacaa atagggcacc aacaattgct gtaaactact gtcaacctca
     7321 cctatcttat cacgataata ttcgtattta acccgctctt gagcagcatg ctgatcgata
     7381 ataaataagc catctttacc ttgagcaaag agataagttc cgtgcatctg accaaaataa
     7441 tccaattcgg gaaacactga ttgctcttca ttttccaaac gggttagcat ttgagataac
     7501 ttttgtctgt tttttaagtc aagattagga tgatcagtct cgataaaggt attctgggga
     7561 cgacttgcgt gtttaaccga agaaggtcct ataacttctt ctgagtgggg taacagctct
     7621 gctttttcaa cctttacact gttgtcaaca gcgccgaaat aaaaatcagt ttcaggtatt
     7681 ttttccgaga cagccgactc tttgacaaaa aagtcattct tttgaggatc ataataaagt
     7741 cccctaaact gtaaaggaag ttgtgtttgc tctggtttag agaaatgtcg tgtgcttgat
     7801 ttggctaagt tttccagagc atctggaatc aaatcttgtt ccttaagact ttcagaaatc
     7861 gccgtactaa ttaaagccat caactcacgc tcttttgaaa tacgaacctc ttgttttgtg
     7921 ggatgaacat tgacatcggc caaataggga tcaatctgaa tatcaatcac aacaattggg
     7981 aaacgtccaa ccatgagctt agaaccgtaa ccatcaagaa ttgctcgatt aagcaagaaa
     8041 ttcttgatgt aacgcccatt aaccaaaatc gtcatgtaat tacggttggc gcgtgtcaac
     8101 tctggcaagc taacatagcc ggaaacttca aaatccaaat cagcattaga gatagccagc
     8161 atttttttgg tagtattcaa accatagatc cctgcaattg cttggcgtaa atcgccagtt
     8221 cctgatgtct gagttaattg gcgaccatca ctaatcagtg tgaaagcaac ttctggatgt
     8281 gccaaactca accggttgac cacatctaca atgtgagcta attctgcctg taaacttttc
     8341 atatatttga gacgagcagg cgtgttgtaa aaaaggtttt caaccttaat tttggttcca
     8401 gtaggagttg agatcgcttc aagtgtctca atttccccac cagtagctat aagaagagaa
     8461 ccgtgagtga cttcttttgt tgctgttttt atcgtgattt tactgataga ggcaacagac
     8521 ggtaaagcct ctccacggaa gccaagtgtt ctaattctaa acaaatcact ctgactctta
     8581 attttactag tagcgtggcg acgcagactt aaaggtaaat cttcatgaga cattccctca
     8641 ccgttgtctg taacctgtat catcttaagg ccagactctt caatttcaac ggtaatctgg
     8701 ctacttttag catcaatagc attctcaacc aattccttga caacactcgc tggcctttct
     8761 acaacttcac cagctgcaat ttggttggcg agaacttccg gtaattcaat aatgtttgtc
     8821 ataattttgt ttttaacact ttctaaacgt tgatataaca aggtttcatt aggtttacac
     8881 ctctcaaaat attaatttgt ggactttttt gtggacttat aaattattca tagccttttc
     8941 ataatataaa acagcttctt tttctttgtc cttagaaaga tggccatagg tatccaaagt
     9001 catgctgata ttcgcatgtc ctagacggta ctgaagttcc ttataactaa tacctgcgtt
     9061 cagcaataaa ctagcgtgag tgtgtctaaa agcgtgaaag gtaaagcgtt caatccctgc
     9121 ttccttacac ctagtatcta aagcgctttg tctaaccgaa gcattaaaat actttcgtgt
     9181 tggtgtggca aacactacct ccgacacacg cgcaccaacc tcattaaata attgtcgctg
     9241 tctcatttga tagagcttta ggcttctaag ggtcttttta tcaacactaa tcactctatt
     9301 gcctgactgg gttttagctg tactcaaaaa cttcaaattt ttattgtaag tcttattaat
     9361 ggctattgta ccattttcta ggtcaatatc tccccactct aatgcacagg cttcgcctat
     9421 cctcaagcca gtggataata aaagttggta gagaactgca tcaaagtaaa ggttatagcg
     9481 tttgtgttga ctagtttcta aatgttctaa aaaagatttc aaatcagacg gatcaataaa
     9541 cttaactttt ttcttagcgt ttggctgctt ccttggaagg ataatatctc ttgctgagtt
     9601 ataatctatt agccctagca atactccttg ttggagaact tttcggatga ctgaacgtac
     9661 cgcagtataa ttgacgtaat atttagataa tctattaatc agcatttgaa tatcactagc
     9721 ggttatctta tcaaccttca tattgcccag agttggcata atatgtcgat taagtctagt
     9781 aacagtagca tcataagttt gaggctttac tgttaactta taggtttcaa gccataaatg
     9841 actaagttct ttaaatgttt taatcacaac ttttctttta attgtagatc cattagatag
     9901 gaagtcaaac tgcgcgtgct tggctttttg attaacttct tttcttgttc ttcctgtgat
     9961 gcttgttttt actctcttac ctgtcatttg gtcaatgcct agataaatac tagcacgata
    10021 aacaattgta ccgtttttct tcttatgttc tgttattttc atgatcataa acctttccat
    10081 cagcaggcaa gctattatta aaagggtttt agattagttc atatcatggt ataataatgg
    10141 tatcgggtaa atatccgaaa tattataaaa gaaaatcatg gtataatatt agtcttttta
    10201 agaccttgcg agcgtggaaa acttgcgtgg tcttttttgt ttggttttgt aaggtacaga
    10261 gagtttcaga agaagttttg catgtctaaa aactaggtca aggtagtcgt agccttatgt
    10321 ctggaggtgg tgcttatgta tgtaagtcac agaaaccaaa agaaaggaga cgacaaggtt
    10381 tgtcagttta tgaaacgctt actttgatga ttgcctttgg tactctgata gtagctatca
    10441 tgaataacaa gaacaaatag aaaaaccgtc ccacacttta gccaatcgtg gacggtttat
    10501 agaaaatata aacaaatacg gtaaggctgc cgtcttaaac ggtgctacca caggagattg
    10561 tttgcgcaat ctcctttttc tatgtccatt atagctttgt ttgataaaga agtcaaaagg
    10621 gtgtagttag aaagtacagt ctttagcgct cttaacattc cttagtatta ccaaaatgaa
    10681 taaaaataat tttatgcaag aatttataat actttatgca accactgatc acgcgcagat
    10741 agttttttta attaccgccg acaaaaagtt cagaaaagtt cagattatac ctcaattttt
    10801 gccaagtttt gagggtcttg taattaaaga aaattaaagg cgtatcaagg tatttgcgcg
    10861 taggtaaaga tgataaattt ggcatttaga catgaaaatt gaacaggtca taaaatgagc
    10921 ggtaacggta aggttacagt aaggtagcta attttattcc acgcgcataa tgctgaactt
    10981 cgagcgaact tagagaatag aaggttacta ctcccaaaca cgaacacatg gtcgcatttc
    11041 ggaatgctgg aaaatgttgg cacgtactga cataatacgt caacctattt caatagttgt
    11101 aaaacgctcg gtaaattcac ttaaagaact tgatatatcg gtcgtttatt aatttacttt
    11161 ttccgttgtg acagcttcta aaatcagttg gcatttttag cgtatagtat tcagaaaatc
    11221 ggacttttcg gctgtcttaa agagcaaaat gcgagtcctt aaaggtaaag caaccaaaat
    11281 tagtggaaaa tagtggaatt cggcgcgtgg gtatcagata gtcatgattt agatgtaaaa
    11341 ctcccaataa cttctccgat aatacggaaa tcactatctc tatctatgag tatatcgtca
    11401 tacttgtcgt tcagactatg cagaaacgct ccagaatcgt ttataacaag ttgtttgata
    11461 taagcatcac cgtaatactc aaaaactcct atatcaccat ctgacaggtt aatagatagt
    11521 ttgataaata catagtcccc agaatggtat tcgggttcca tagaatcgcc ataaaccggt
    11581 atgacaaaat cagcgtcgta atcgactggt aattcaattg tttctacttg tacatcattt
    11641 agatactgac ctgtaccagc tgaagctgcg tggtcgtagt agttgtaagt gtagtaggtg
    11701 gcttgtggtt cttctactgt gttttgttgt tctaattgtg tttcagcata atcaaggacg
    11761 tttaattgtc gcttgtgttc tagctgagat aaagtagatg ttactttttg tagggttgat
    11821 gggataatat tactatttgt ttcaatatta ttagaatcaa aacgggggtc aatgtctgat
    11881 tttagaactc cgaagaagtc cgccagcttt tgaacgtttc cagcagttgg gagtgaacga
    11941 cctttaacat agccagttat cgtactctta ggtattccta gtttgttgtg aatatctatc
    12001 tgtttgatat tctttttagc cataagcatg ttgagatttg ttgagaaaat ttctctattt
    12061 attaaatctt ggggactatt tttagccatt tctcttaccc ctttcttttt gtatctgaat
    12121 taattctaac acaaataaac ctaaaagtac tattttttta atatttttct acaaaaatca
    12181 ttgacttggt acgaaaataa tagtactatt aagccatagc tttttagaaa ggagttgaaa
    12241 acatgacgca aattacacta aaagctgccc gaatcaacgc tggttacact ttaaaacaag
    12301 tagctggagc ggttgggaaa aatccccaaa ctatttcaaa gtacgaaaaa gatagctccg
    12361 atatttcttt aggactattg caaaaattat catctttgta cggtgtcact attgataacc
    12421 tttttttagg taaaaaagta cgaaaataat agtactttat taattagaaa ggcaggcaag
    12481 aaatgacagt gggtgacaaa atcaaaacta ttcgccttaa cttaggagaa actatggagg
    12541 aattcggaaa acgctttggc acttctaaag gtactgttaa taattgggag aaaggaagaa
    12601 acctcccgaa tagaaacagt ctattaagaa tagcacagct cgctaatcaa actgttagcg
    12661 acttcttagc tatcaccgat taagaaaggc aggtaaataa caaatggcta gaacaccatt
    12721 tacacaagaa ttattacatc agatatttga cgatacagga acaatgagcc tagagctgat
    12781 tgctgagcgg cttcccgatt ggtctgaaaa agacatcaag cttaggctgg cagcttggcg
    12841 gtatcgaaat aacattgatt acaccatggc aaacggtgaa attgatacct ttgaaattat
    12901 caataacaga aaagccatat ccgaagaagt ttccgcagga aggcaactta agcttgaaga
    12961 gtatttcaag caagtacagg caacggctga aattattaat aaaccgactg ctagcgacac
    13021 caatcgttta aaggccattc aattacaaca agtggctatg gatgaaattc ctgatcagta
    13081 ttttaaagaa ctaacggaac tctatggata aaggaggatt accatgaacc tagtttacat
    13141 ggatggtaag aaagagcctt acaccacaca cgacatcata gctgaacatg ctgaaattga
    13201 cattatttca gttagaaagc tgattgataa gcataaaaag gattaagaac gaaagtgctg
    13261 ttgtgtggta cataaggtaa cgcaccccct tttttactac gtaacctcta aaacgttgat
    13321 atagatagcg ttagagaagg atggttacgc agtaacgcaa aatcacccta ccctacccct
    13381 atatataaat aaataattaa taaatcatta attagactta ttgtttgtta ctgcgtaacc
    13441 tttcttaaat aaatgctgaa atcgattggt actaaagaga ttgagtaggt tacgcaacat
    13501 ggaaaattct gcgtaacttt gcgtgaccat gcgttacctt ttagaagcgt ttggggtttt
    13561 gtcatttgaa atccataaac ctgaaaaagg ttcactaggc ggacgaccta gaagaattta
    13621 tcacttaaac gaacagcagg cgactttatt agttacctat ctaggaaata ccgaaccagt
    13681 cagagagttt aaaaagaacc tagtcaaggc attctttgaa atgcgagaag aactcactca
    13741 aattaggtta cagcgttctc ttgaagctcc taaacgaaaa acacttaatg aagctatcaa
    13801 gacatgggaa cacgcgccta aaatggctta tccgactgtg tacaatctat tgcttaaagc
    13861 tgttactggt aagaatagca agcaattaaa agtaaccaga ggtggttatt ctgatattga
    13921 ctgcttaaac tcaattgaac tagcacagta cactgcttta gaagatatgg caatcgctct
    13981 tatcaatctt aattttacct atcaagatat taagacaatg gcattaaaaa acacgctaca
    14041 atgcgcgtga aaacaacaaa aaaggctttg acagcgacca aacttccaag ccttcaacta
    14101 gtataactaa actcaattaa taaagcaggc aagctattat taaaggggtt ttagtaaata
    14161 tttgatactt ccattgtatc atactgatca ttgaaaataa aatagagtac gcaaaaaaag
    14221 tttggtttag gggttgacaa ttattgacga cataaactat aatagttttt gtcgacatga
    14281 aaggagtgat aaactcaatg accgacacca aaaaacgagt aggacgacca aagggagaaa
    14341 actcaaatac tgttaaatta acggtaaggg ttagtgaaga gttagatagc aaactaaata
    14401 agtacgctaa agagaataac ttgactaaac ccgagacttt gagaaaagcc tttgaaaatc
    14461 ttacaaaata aaaaagtact ctaccacgtt tacaatcgcc aaaccgtcaa cgtgatagag
    14521 ccagcaccca caaagtaggt acgtaaatag tatatcatgc gtactctatt ttgtgaacca
    14581 tttgcacatt gaaaatagaa tacgcatgtt tttttaattt aggggttgac ttatgtacgt
    14641 gcttatcata tgatataagc acgggcttaa atgaaaggag gaactcctat gagtccaaaa
    14701 atgggcagac ctgtaaaagg tacagctaaa cgtgataaac ggctggaagt tcgtttgacc
    14761 gctgacgagt ataatacaat acaagaaaca gcggataaaa atggattatc taaagcggat
    14821 ttaatcgtta aagctgtaaa ctcttatgag tctgaaaaat aaaaaagttc ctaacgtggt
    14881 atagtttggc gaccgtacac gttaggaact cccagcaccc acaaagtagg tacgtaaata
    14941 gtatatcatg cgtactctat tttgtgaacc taaaattcat agaaagggag tgcgcttttt
    15001 gtgtgctcaa aaatcaagac aatatgatta agaaaaaaag aaatccgaga gtgttattta
    15061 gaaacgtcgc ttataaacta tctgaaattg aaggaaaaac attaacagaa atcgcttctt
    15121 ttcttggttt tgggaattca gaagtttgca gaagcaccct atataaatgg aagcgtaaaa
    15181 aatggctaaa attcgacctc aaaaacggtc attatcgtaa tgttgaagta ttacatgaag
    15241 ttacgcttga aaaaatggct aataaggaat taaaagaaca aggactcatt tataaagcta
    15301 acatttacta tgaacaagtg gtctcaacat ctgaaattat agaagacatt aaaaccaaaa
    15361 cacaagatag aattaaagcc atccacttac aacaaaaagc gctagagcgt atccctagcg
    15421 agttattcgc agaattatac actaacatga actaatcagg gagctacccc ttaaaactag
    15481 catgaatcta gtataagaaa caagcaatca ataaaaacac tacacagaaa ataagaatta
    15541 ataa
//
LOCUS       DATXQK010000037        15528 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.037, whole genome shotgun sequence.
ACCESSION   DATXQK010000037 DATXQK010000000
VERSION     DATXQK010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15528
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.037"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(22..2013)
                     /gene="uvrB"
                     /locus_tag="VC394_001283"
     CDS             complement(22..2013)
                     /gene="uvrB"
                     /locus_tag="VC394_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEP5994752.1"
                     /translation="MIDKRDDKPFKLKSKYKPSGDQPQAIESLVDNIEGGEKAQILLG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPDNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVVASVSCIYGLGSPKEYA
                     DSAVSLRPGQEISRDTLLNQLVDIQFERNDIDFQRGCFRVRGDVVEVFPASRDEHAFR
                     VEFFGDEIDRICEIESLTGKTIGEVDHLVLFPATHFVTNDEHMEQSIAKIQAELAEQL
                     QLFESEGKLLEAQRLRQRTEYDIEMLREMGYTSGVENYSRHMDGRSPGEPPYTLLDFF
                     PEDFLIMIDESHMTMGQIKGMYNGDQARKQMLVDYGFRLPSALDNRPLRREEFESHVH
                     QIIYVSATPGEYEMSQTNTIIEQIIRPTGLLDPEIDVRPSMGQMDDLLGEINQRVARD
                     ERTFITTLTKKMAEDLTDYLKEMGVKVKYMHSDIKTLERTEIIRDLRLGVFDVLIGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNVDGHVIMYADKMTDSMQ
                     RAIDETARRREIQIAYNKAHGIVPQTIKKDIRGLISISKTSHNDISKEEMDYESMSRG
                     ERKEAINALQKQMQEAAELLDFELAAQMRDLILELKLMD"
     gene            2205..4379
                     /locus_tag="VC394_001284"
     CDS             2205..4379
                     /locus_tag="VC394_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEP5994753.1"
                     /translation="MTHKIKVLLLAIMSIFLTCNIASAETIAIVSDTAYAPFEFKDSD
                     QIYKGIDVDIINEVAKRQSWDFSMSFPGFDAAVNAVQSGQASALMAGTTITNARKKVF
                     HFSEPYYDTKIVIATRKANAIKKYSDLKGKTVGVKNGTAAQAFLNNYKKKYDYTVKTF
                     DTGDLMYNSLSAGSIAAVMDDEAVIQYAISQNQDIAINMKGEPIGSFGFAVKKGSGYD
                     YLVNDFNTALKAMKADGTYQAIMTKWLGTDDKATTSQATGNPSAKATPIKDSYKIVSD
                     SSFAPFEFQNGKGKYVGIDIELIKAIAKQQGFKIEIANPGFDAALNAVQSSQADGVIA
                     GATITDARKAIFDFSDPYYTSNIILAVKAGKNIKNYEDLDRKTVGAKNGTSSYSWLKE
                     NAPKYGYNVKAFDDGSSMYDSLNSGSVDAIMDDEAVLKYAISQGRRFETPLEGISTGE
                     VGFAVKKGTNPELIEMFNNGLAALKKSGQYDDIIDKYLDSKKAATPSEKGADESTISG
                     LLSNNYKQLLAGLGTTLSLTLISFAIAIIIGIIFGMMAVSPTKSLRLISTVFVDIVRG
                     IPLMIVAAFIFWGVPNLIESMTGHQSPINDFLAATIALSLNGGAYIAEIVRGGIEAVP
                     AGQMEASRSLGLSYGTTMRKVILPQAVKLMLPNFINQFVISLKDTTIVSAIGLVELFQ
                     TGKIIIARNYQSFRMYAILAIIYLIMITLLTRLAKRLEKRLN"
     gene            4379..5119
                     /locus_tag="VC394_001285"
     CDS             4379..5119
                     /locus_tag="VC394_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003107174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEP5994754.1"
                     /translation="MTELKIDVQDLHKSYGQNEVLKGIDAKFYEGDVVCIIGPSGSGK
                     STFLRTLNLLETITSGKVVVDGFELSDPKTNIDKARENIGMVFQHFNLFPHMTVLENI
                     IFAPVELGKESKEAAKKHGMALLEKVGLSDKADAFPGSLSGGQKQRVAIARSLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKDLAEQGMTMLIVTHEMGFARQVANRVIFTDGGQF
                     LEDGTPEEIFDHPKHPRLIEFLDKVLNV"
     gene            complement(5267..5419)
                     /locus_tag="VC394_001286"
     CDS             complement(5267..5419)
                     /locus_tag="VC394_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994755.1"
                     /translation="MTEKNGATFLMAVGFFLIAISPMVGNKYLHIVTGLALTGLGYYL
                     TKHKRP"
     gene            complement(5416..6795)
                     /locus_tag="VC394_001287"
     CDS             complement(5416..6795)
                     /locus_tag="VC394_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEP5994756.1"
                     /translation="MSHFSDKFMEISGKIGSQRHLVAIRDSFISMMPITMAGSVAVLL
                     NVFLRDIPNNMGWTGFAKAMQPVIDINGYVYFGTIGIMALFFAFAFGYNLAVMHKINP
                     LAGGLISFGSFIATLPQTLTISTPLENASANLISNLKEMGLSVVTAGGASSIETSQWG
                     AIALKYVGATGLFTALIIGFLSSFVYAALTKRNITITLPDNVPPAVNKAFAAIIPGTV
                     AIYASAIFAYLIFALTGSSLSDVISTYIQLPLLGLSQGIGSVILLTFLVQLLWFFGLH
                     GHNVLAPVMDGIYMVALTENTAAYNTAHSAANLPYLWTRGSFDAYAQMGGSGVTLALI
                     IAIFIFSKREEHKTIAKLSAPMGIFNINEPITFGMPIVLNPTFVIPWLIVPPICASIA
                     YFATAIGLIPPVFLSVPWITPVGLYAYLATGGNIMAGLVSLFNLFVAFLIWAPFVILA
                     NKEKASDLA"
     gene            complement(6973..7422)
                     /locus_tag="VC394_001288"
     CDS             complement(6973..7422)
                     /locus_tag="VC394_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HEP5994757.1"
                     /translation="MIISAEGDVSVHHCFEAMLISLQEDYRQNTLRQLSRDDIKAGIN
                     YVKHYGKYKERTVRVLITALEAPQLYTACFSSNHGIKELAYRLEALGENRTRITYSYD
                     YHPLDIFQKANYFIVGKLFKKSLERQSQAQLAALIQYAKQLQQPSSL"
     gene            complement(7419..7754)
                     /locus_tag="VC394_001289"
     CDS             complement(7419..7754)
                     /locus_tag="VC394_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEP5994758.1"
                     /translation="MTEPSNLEAIMGLIMYGGEAKGKAVEAIHAARDGQFEEAQHLLQ
                     EATNSLNIAHKSQTHLLSQEAAGDAVELSLLMVHGQDHMMTALAFIDLAKELVTVYDK
                     MSAIQRGES"
     gene            complement(7757..8068)
                     /locus_tag="VC394_001290"
     CDS             complement(7757..8068)
                     /locus_tag="VC394_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEP5994759.1"
                     /translation="MKSIMLVCNAGMSTSMLVTKMQKAAQARDLEVSIWAVPVSEADN
                     EVAANSIDVLLLGPQVKFLLKDFKDKFEPDIKVDAINMADYGLMNGEKVLETALAMME
                     E"
     gene            complement(8091..10085)
                     /locus_tag="VC394_001291"
     CDS             complement(8091..10085)
                     /locus_tag="VC394_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003061111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEP5994760.1"
                     /translation="MLSKKELAIITFLIHHKEQFVSSATLAEVIGMSDRTVRKYLKEL
                     ISSLPEHGAHIISKQGRGYCLEIDHSMAFDIFWQESVTSKKRLADVTQVEESVDREDY
                     VLHKFFFEDAVQDFEELCQELYISRTTLKHVLAGIKERIIPYQLELEITHQHIQIRGK
                     EEDIRHFIMDYFFVTSFDNTLSTMVGNTFLEGINFAEIMIIVLDECRDAKLKLSDFVM
                     NNLVLHIALMVQRIRSGCPLELFSIPIAIRQSDEYQVALRILYRVEEVMGIRFPKEEA
                     NYIALHLKVKHSVGKHWQDDNTDEKLQDHLKACIAKISQLTGMTLETDTNLFQGLLAH
                     MMPLTTRLENHIQLTNPLTEEIKSQYPEIFTLTKQTFSDLLVCQKNDVSDDEWAYISL
                     HLMAAIERYSNRHKLRVLVVCATGYGSAMMLKNRLEKEFEGRLRIVDVISYYEITEER
                     LKTVDLIISSISLANLMFLTPVITVSVFLSNQDIETIRQFIGEQEGIKKEVSLPSQMS
                     LAKAEQLLTGVFSPNRFLYLDEKISKEDLLLRMIACLDEAGTETFVEDFYHQMVLREN
                     YSPVIYGEVLAFPHPANPMTYSEQVVVAICREPLEWDKAHQAVHFVFLLSPSKGHNHR
                     LKYVSPGLASFVNQVELQQALLEEPNYSKFMTVFTPLIHD"
     gene            complement(10191..11285)
                     /locus_tag="VC394_001292"
     CDS             complement(10191..11285)
                     /locus_tag="VC394_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEP5994761.1"
                     /translation="MVDLGFSLYPERYDVTKSKAYIDLCHSYGAKRLFMSLLQLAPAD
                     HQMFHCYAELIAYANQLGIRVIADVSPSFISQAGWSDQLIERAHAFGLAGLRLDEALP
                     LAEIVTLTRNPFGLKIELNMSTDKQLLMSLLATDAEQSNIIGCHNFYPHEFTGLSWQH
                     FKDMSRFYHEHDIETAAFITAQSASEGPWLLAEGLPTVEDHRHLPIGLQVELMKAIGT
                     IDNILISNQFISEEELAACTQALARPVTTIKVRPIIDLTEVEEKIIGYPHCYRGDVSD
                     YVIRSTMPRLVYAQESIAPRDQSKEVKRGSIIIDNDRYHRYKGELQIALKNFTVSSKA
                     NVVAEVREDYLSLLDDLRPWQEFCLEIDPS"
     gene            complement(11294..12694)
                     /locus_tag="VC394_001293"
     CDS             complement(11294..12694)
                     /locus_tag="VC394_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEP5994762.1"
                     /translation="MKQRKHRYQFPDGFLWGSSTSGPQSEGTVPGDGKGPSNWDYWFS
                     IESAKFHHQIGPEKTSTFYENYKGDIALLKETGHTIFRTSIQWSRLIPEGVGEVNPKA
                     VTFYREVFQDIIAQGIKLIVNLYHFDLPYALQEKGGWENKATVWAYETYAKTCFELFG
                     DLVNTWITFNEPIVPVECGYLGYYHYPCKVDAKAAVQVAYNTQLASSLAVKACHKLHP
                     DHKISIVLNMTPAYPRSDAPEDVKAARIAELFQTKSFLDPSVLGVYPAELVSILEEAD
                     LLPQYSADELEVIKNNTVDFLGVNYYQPLRVQAPSKSQQEGDPLILDIYFEPYDMPGK
                     KVNPHRGWEIYEPGLYDIALNLKEHYGNIEWLVTENGMGVEGEEAFLADGQIQDDYRI
                     TFIEDHLIQLHKALGEGANCKGYLLWTFIDCWSWLNAYKNRYGLVALDLESQKRTIKK
                     SGYWFKALSESNGFDK"
     gene            12919..13614
                     /locus_tag="VC394_001294"
     CDS             12919..13614
                     /locus_tag="VC394_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEP5994763.1"
                     /translation="MAYLNYFTKTEWALWLSSVLAILISALLFGKQAPLALIASLIGV
                     TSLIFSAKANPIGQGLVIIFSIIYAYLSLRNSYYGELMTYLFMTLPMTIFSLFTWLNH
                     PFEGKKSQVTISRLTPTDRRCLFVFTILVTLIFYGILASIQTAYLLVSTLSIATSFSA
                     VYLSYKRSPYFALAYGLNDLVLILLWMHAAQTDASQYAVVICFATFLINDSYTFYSWL
                     MLQRYQEKKVKKG"
     gene            complement(13619..13777)
                     /locus_tag="VC394_001295"
     CDS             complement(13619..13777)
                     /locus_tag="VC394_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994764.1"
                     /translation="MGDKPISFKDKDGNFVSAADVWNAEKLEELFNLLNPNRRLRLER
                     EKLKKDEA"
     gene            complement(13846..15159)
                     /gene="obgE"
                     /locus_tag="VC394_001296"
     CDS             complement(13846..15159)
                     /gene="obgE"
                     /locus_tag="VC394_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEP5994765.1"
                     /translation="MSMFLDTAKISVQAGRGGDGMVAFRREKYVPNGGPWGGDGGKGG
                     SVIFRVNEGLRTLMDFRYNRKFKAKSGEKGMTKGMHGRGAEDLIVFVPQGTTVRDAET
                     GKVITDLVEHGQEVVIAKGGRGGRGNIRFATPRNPAPEIAENGEPGEERQLELELKIL
                     ADVGLVGFPSVGKSTLLSVVSSAKPKIGAYHFTTIVPNLGMVRTKSGDSFAMADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSASEGRDPYEDYVSINNELETYNLRLME
                     RPQIIVANKMDMPEAQENLKAFKKKLAAQYDEFDDLPMIFPISSLAHQGLENLLEATA
                     ELLAKTDEFLLYDESDLVDEEAYYGFAEAEKEFEITRDDDATWVLSGEKLERLFVMTN
                     MERDESIMKFARQLRGMGVDEALRERGAKDGDLVRIGKFEFEFVD"
     gene            complement(15216..15344)
                     /locus_tag="VC394_001297"
     CDS             complement(15216..15344)
                     /locus_tag="VC394_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEP5994766.1"
                     /translation="MAFGENGPRKKTTFEKVTMVVVILMVLVTVGGLIASALSVLM"
BASE COUNT         4667 a         3326 c         2791 g         4744 t
ORIGIN      
        1 aatataagct ttattcaaaa cctagtccat taatttaagt tctaaaatca agtctcgcat
       61 ttgagctgcc aattcgaaat caagtaattc agctgcctct tgcatttgtt tttgcagagc
      121 attgatcgct tcttttcgtt ctcctcttga catactttca taatccatct cttctttcga
      181 tatatcattg tggcttgttt tacttatact aataaggcct ctgatgtctt tttttatagt
      241 ttgtggaacg atgccgtgcg ctttgttata tgctatttga atctcacgac gacgagccgt
      301 ttcgtcaata gcccgctgca tagaatctgt catcttgtcc gcatacataa tgacatgacc
      361 atctacgttt cgagctgctc taccgatggt ctgaatcaag ccgcgttcat tacggaggaa
      421 accctcttta tcagcatcca aaatggctac aagactaact tctggcacat caatcccttc
      481 acggagcaga ttgattccaa tcaagacatc aaaaacacca agtcgtaaat cacgaataat
      541 ttctgtccgc tctaaggttt taatatcact gtgcatatac ttgaccttga cacccatctc
      601 ttttagataa tcagtcaaat cttctgccat ttttttagtt aaggttgtga tgaacgtccg
      661 ttcatcgcgc gctacgcgct ggttaatttc tcccaacaaa tcatccattt gccccatact
      721 tggccttaca tcaatttcag gatcaagtag cccagtcgga cgaataattt gctcaataat
      781 tgtattagtc tgtgacattt cgtattcacc aggcgttgct gaaacataga taatttggtg
      841 cacatgactt tcaaattcct cgcgtcttaa gggacgatta tcaagagctg atggcaatcg
      901 aaaaccatag tcaactagca tttgttttct ggcttggtca ccattataca tgcctttgat
      961 ttgtcccatt gtcatgtgac tttcatcaat cataattaaa aaatcttctg ggaagaaatc
     1021 taacaaggta taaggaggtt ctcctggtga cctgccatcc atatgacggg aatagttttc
     1081 gactccgcta gtatagccca tttcacgtaa catttcaata tcatattccg ttctttggcg
     1141 taatcgctgt gcttctagta gtttaccttc agattcaaat aactgtaact gctctgctaa
     1201 ctctgcttga attttggcta ttgattgctc catatgctca tcatttgtta caaagtgagt
     1261 agctggaaat aaaaccaaat ggtcaacttc tccaatagtt ttcccagtta aactttcgat
     1321 ttcacatata cgatcaatct catccccgaa aaattccacc cgaaaagcat gttcatccct
     1381 ggatgctgga aagacctcaa caacatcgcc acgtacacga aaacagccgc gttgaaaatc
     1441 aatatcatta cgctcaaatt gaatatctac taattgattt agcaaagtgt cacgtgatat
     1501 ctcctgacct ggacgtaggc tgactgctga atctgcgtac tcttttggcg aacctagtcc
     1561 ataaatacaa gatacagaag ctaccactat gacatcgtta cgctcaagta aagaagaggt
     1621 ggctgaatgg cgaagtttat cgatttcatc attgactgaa ctatcttttt cgatataggt
     1681 gtcgcttgat gggacatatg cttcaggttg gtagtaatca taataggaaa caaagtattc
     1741 cacagcattg tctggaaaaa attctttaaa ttcaccatat aattgtcctg ctaaggtttt
     1801 attgtgagct atcactaagg taggtttatt tactttacta attacttggc tcatggtgta
     1861 tgtttttccc gttcctgtag cacctaggag aatctgagct ttttctccgc cctcaatatt
     1921 atcaactagg ctttcaatag cttgtggctg atcaccagac ggtttatact ttgattttag
     1981 tttaaatggt ttatcatcac gtttatcaat cattttatac cctctaatag tcttatctct
     2041 tatagactat tttaacatag ataagccaga atcacctcca ataagcgtaa aaaacatgtc
     2101 atttacatgt tatgttttat gacatataaa taacaaattt cttgcttaat gttcaacatt
     2161 ttgttataat taaccatatt ttttagtttt ataaggagat tagtatgacg cacaaaataa
     2221 aagtattgct gcttgcgata atgtctattt ttttgacatg caatattgca agtgctgaaa
     2281 ctattgctat tgtttcagat acagcttatg ccccatttga atttaaagac tcagatcaaa
     2341 tttacaaagg aattgacgtt gatattatta atgaagtagc caaacgtcaa tcttgggatt
     2401 tcagtatgag tttcccgggt tttgatgcag ctgtaaatgc tgtacaatct ggtcaagcga
     2461 gtgctctaat ggccggtaca accattacga atgctcgtaa gaaagtcttt catttctcag
     2521 agccatatta cgataccaaa attgtcattg cgacacgtaa agccaatgcc atcaaaaaat
     2581 acagtgactt aaaaggaaaa acggtcggtg ttaaaaatgg aacagcggct caagcctttt
     2641 tgaataacta taaaaaaaag tatgattata ctgttaaaac atttgacaca ggtgatctta
     2701 tgtataatag tttatctgct ggttctattg ccgctgttat ggatgatgaa gcggttatcc
     2761 aatacgcaat cagccaaaac caagatattg ctattaacat gaaaggagag cccattggaa
     2821 gctttgggtt tgctgtcaaa aagggaagcg gatatgatta tctagttaat gatttcaata
     2881 cagctcttaa agctatgaaa gctgatggta cctaccaagc tatcatgacc aagtggttag
     2941 gcacagatga taaagctacc accagtcagg caacgggaaa tccatctgcc aaagctacac
     3001 ctataaagga cagttataaa attgtctctg attcgtcttt tgcacctttt gaatttcaaa
     3061 atggtaaggg caaatacgtt ggtattgaca tagaattaat caaagctatt gctaaacaac
     3121 aaggtttcaa aattgaaatc gctaatccag gtttcgatgc tgccttaaat gctgtgcaat
     3181 ctagccaagc agatggggtc attgctggtg caactattac tgacgctcgt aaagctatct
     3241 ttgatttttc tgatccttat tatacttcta atatcatttt agctgttaaa gctggaaaaa
     3301 acatcaagaa ctatgaagac ttagacagaa aaacagtcgg tgctaaaaac ggcacttcat
     3361 cttactcttg gttaaaagaa aacgctccta aatatggtta taatgtcaag gcatttgatg
     3421 atggttctag catgtatgat agcttaaatt caggttctgt agatgctatc atggatgatg
     3481 aggcggttct taaatacgct atttctcaag gtcgtcgctt tgaaacacct cttgagggca
     3541 tttctactgg tgaagttggt tttgctgtca agaaaggaac taatccagaa ttaatcgaaa
     3601 tgttcaacaa tggcttagct gctctcaaaa aatctggtca gtatgatgac attatagata
     3661 aataccttga ctctaagaaa gctgcaactc cttctgaaaa aggtgctgat gagtctacta
     3721 tttcaggcct attatcaaat aactacaaac aactattggc aggacttgga accacgctca
     3781 gtttaaccct tatttcattt gctattgcta taattatcgg gatcatcttt gggatgatgg
     3841 ccgtgtcacc aactaaatca cttcgactca tttcaacggt ctttgtagac attgttcgag
     3901 ggattccttt gatgattgtg gctgccttca ttttctgggg agtaccaaac cttatcgaga
     3961 gtatgaccgg ccaccagtca ccgattaatg atttcttagc tgctacaatt gcactgtcac
     4021 ttaatggcgg agcctatatt gctgaaattg ttcgcggtgg tatcgaagct gttccagcag
     4081 ggcaaatgga agctagtcga agtcttggtt tgtcttacgg aaccacgatg agaaaagtaa
     4141 ttctcccaca agctgtgaaa ctaatgttac ctaactttat caatcagttt gttatttcat
     4201 tgaaggatac aacaatcgtc tcagcaattg gtttagtgga actcttccaa acaggtaaaa
     4261 tcattattgc tagaaattac cagtcgttcc gtatgtatgc tattttagca attatttacc
     4321 ttatcatgat tacactctta acaagacttg caaaacgttt agaaaagagg cttaactaat
     4381 gacagaatta aaaattgatg ttcaagattt acacaaatct tatggtcaaa atgaagtctt
     4441 aaaaggaatt gatgccaaat tctacgaagg tgatgtcgtt tgtatcattg gtccttctgg
     4501 ttctgggaaa tcaacctttc tacgtaccct caaccttcta gagacgatta ccagtggtaa
     4561 ggtcgtggtg gacggttttg aattatcaga tcctaagact aatattgaca aggcacgtga
     4621 aaatatcggg atggtattcc aacacttcaa ccttttccct cacatgactg ttcttgagaa
     4681 tattatcttt gctcctgttg aattaggtaa agagtctaaa gaagctgcta aaaagcacgg
     4741 catggcactt ctagaaaaag tcggactttc tgataaggcc gacgccttcc caggaagttt
     4801 atccggcggg caaaaacagc gtgtcgccat cgccagaagt ttagcaatga acccagatat
     4861 tatgctcttt gatgaaccaa cttctgctct tgaccccgaa atggttgggg acgttttgaa
     4921 cgttatgaaa gacttggcag aacaaggcat gaccatgttg attgttactc acgaaatggg
     4981 gtttgctcgt caagtagcta accgcgttat ctttacagat ggtggtcaat ttttggaaga
     5041 tggaactcct gaagaaattt ttgaccatcc taagcaccct cgtctcattg aatttttaga
     5101 caaggtctta aacgtctaat agagcagaca ccatactaac ttccaaaaaa taataaggca
     5161 aggaaacagg tttccttgcc ttttttggtt actgttatta ttcaaaccag caaaataggg
     5221 atactcttat gactctctac ttcataagag tgtcccctat gcttttttat gggcgtttat
     5281 gtttagtgag gtaataacct aatcccgtaa gagctagtcc cgtcactatg tgtaagtatt
     5341 tgttaccaac catcggacta atggcaatga gaaaaaagcc aacagccatt aaaaaagttg
     5401 ctccattttt ttctgtcatg caaggtcact cgctttttct ttattggcca aaatgacaaa
     5461 tggtgcccag ataaggaagg caacaaagag gttaaacagt gaaaccaaac ctgccataat
     5521 attacctcca gtagctaggt aagcataaag tccaacaggg gtaatccaag gtactgataa
     5581 gaataccggt ggaatcaaac ctattgcagt agcaaaataa gctatgctag cacaaattgg
     5641 tggcacaatt agccatggaa tcacaaaggt tggattcaaa acaattggca tcccaaatgt
     5701 aattggttcg ttgatattga aaatacccat tggtgcggac aatttggcaa tggttttatg
     5761 ctcttctcgt tttgagaata tgaagatagc aataatcaat gccaaggtta ctcctgaacc
     5821 acccatttga gcataagcat caaaggaccc gcgtgtccaa aggtatggta aattagcagc
     5881 gctatgcgct gtattgtaag cagctgtatt ttctgttaaa gctaccatat aaataccatc
     5941 catcactggg gctaagacat tatgaccatg caagccgaag aaccagagca gttgaactaa
     6001 gaaggttaac aagataactg aaccaatacc ctgtgataag cctagcaatg gtaattggat
     6061 ataggttgaa atgacatcac ttagggaaga acctgttaat gcgaagatga ggtaggcgaa
     6121 aattgccgaa gcgtaaatcg caactgttcc tggaataatg gcagcaaagg ctttattaac
     6181 cgcaggtggt acattatctg gtaaggtaat ggtaatatta cgtttggtca aagccgcata
     6241 cacaaaactt gataaaaagc caataattaa agctgtgaac aagcctgtgg caccgacata
     6301 ctttagagct attgctcccc attgactagt ttcaatactt gaagctccac ccgcagtaac
     6361 gactgaaagc cccatttctt tcaaattgct aattaggtta gcagaggcat tttcaagtgg
     6421 tgtcgaaatg gttaaagtct gaggtagggt ggcaataaat gagccaaaag aaatcaatcc
     6481 tcccgccaat ggattaatct tatgcataac agctaaattg taaccgaaag caaaggcaaa
     6541 aaagagcgcc atgataccaa ttgtcccaaa ataaacatag ccattgatgt cgataacagg
     6601 ttgcatagct tttgcaaacc ctgtccatcc catattgttt ggaatatcgc gtaaaaaaac
     6661 gttcaataag acagcaactg aacctgccat ggtaataggc atcattgaaa taaaggaatc
     6721 acgaatagct actaaatgcc gttgtgaccc aatttttcct gaaatctcca taaacttatc
     6781 agaaaaatgc gacataaatc ttctcctctt tttatttttt taatgtaaca aaggtaacct
     6841 taaaagcagc ggtgtctgcc taacacaatt atctcaaaat catccttgtt aaacgtgatg
     6901 cttagagata accggtaatc ggctcttatc caaatgaacc cttctctctc attcaaggtc
     6961 tatgaataat cttcacaaag acgatggttg ctgcaactgt ttagcatatt ggataagggc
     7021 tgctaactgg gcttgagatt ggcgttctag gctttttttg aacagtttgc caacaataaa
     7081 ataatttgct ttttgaaaaa tatctagagg atgatagtca taagaatagg taattctagt
     7141 ccgattctcc cccagagcct ctaatcgata agccaattcc tttatcccat gattggaact
     7201 aaaacaagca gtatataatt gcggagcttc tagggccgta ataagcaccc tcactgtacg
     7261 ctctttgtat ttaccataat gtttaacata gtttatcccc gctttgatat catctcttga
     7321 caactgtctc aaggtatttt gtcgataatc ttcttggagc gatatcagca tggcttcaaa
     7381 acaatgatgg acggaaacat ctccttctgc tgaaatgatc atgactcacc tctttgtatt
     7441 gctgacattt tgtcgtaaac ggtaaccaat tccttggcta aatcaataaa ggctaaggca
     7501 gtcatcatgt gatcttgccc atgcaccatc aataaagaca attcaaccgc atcaccagct
     7561 gcttcttggg acagcaaatg tgtctgcgat ttatgggcaa tatttaacga gtttgtcgct
     7621 tcttgaagta agtgctgagc ctcctcaaac tgcccatctc gtgctgcatg aatagcctct
     7681 acggccttgc ccttggcttc gcctccatac atgattaagc ccataatagc ttctaagttg
     7741 gatggttccg tcatgcctac tcctccatca tcgctagggc tgtttcaagg actttttcac
     7801 cgttcataag accataatca gccatgttaa tggcatctac tttaatatct ggttcaaact
     7861 tatctttaaa atcctttagt aagaatttga cttgtggacc taacaagaga acgtcaatgc
     7921 tattagcagc cacttcgttg tcagcttcac tcacaggaac tgcccaaata gaaacttcaa
     7981 ggtcacgcgc ttgggctgct ttttgcatct tagtcaccaa catactagtt gacataccgg
     8041 cattacaaac taacataata gatttcataa ggtttctcct tttaatcgtt ttaatcatga
     8101 ataagtgggg taaacactgt catgaatttg gaataatttg gttcttctag taacgcttgc
     8161 tgaagctcca cctgattgac aaaagatgcc aaacctggtg atacatattt taacctatga
     8221 ttatgccctt tagacgggga taatagaaag acaaaatgaa cagcttgatg cgccttgtcc
     8281 cactcaagag gctctcggca aatagccact actacctgtt cagaataagt catgggatta
     8341 gcaggatgcg gaaaggcaag cacttctcca tagatgactg ggctgtaatt ttccctcagt
     8401 accatttgat gataaaagtc ctctacaaac gtttctgttc ccgcctcgtc aagacaagca
     8461 atcatccgta aaagcaagtc ttcctttgag attttctcgt ctaaataaag aaaacgatta
     8521 gggctaaaaa cacctgttag aagctgctct gcttttgcta aactcatctg tgacggtaaa
     8581 ctgacctctt tcttgatacc ttcttgttct ccaataaatt gtcgaatagt ttcaatatcc
     8641 tgatttgaca aaaaaacact gacagtaatc actggtgtta aaaacatcag attggcaagg
     8701 ctgatagagg aaataatcaa atcaacggtt ttcagtcgct cttcggttat ttcatagtaa
     8761 ctgataacat cgacaatacg caaacgtcct tcaaattcct tttccaagcg atttttcaac
     8821 atcatggcac taccataacc tgtggcacaa actactagaa ccctcaattt atgacggttt
     8881 gaataacgct caatggccgc cattaaatgc aaactaatat aagcccattc atcatctgag
     8941 acatcattct tttgacatac aagcaaatcc gaaaaggtct gcttggtaag ggtaaatatt
     9001 tcaggatact ggcttttaat ttcctcagtt aaaggattag tcagctgaat atggttttca
     9061 agacgtgtgg tcaagggcat catgtgggct aataaacctt gaaataaatt ggtatctgtt
     9121 tctaaagtca tgcctgtcaa ttgactaatc ttggcaatac atgcttttaa atggtcctgc
     9181 aacttttcat cggtattatc atcttgccaa tgcttaccaa cagaatgttt gacctttaga
     9241 tgcagtgcaa tataattagc ctcctcttta ggaaaacgta tccccatcac ttcttccact
     9301 cgatacaaaa tgcgcagagc tacttgatac tcatcagact gacgaatagc tattggaatg
     9361 gaaaacagtt ctaatggaca ccccgatcga atacgctgaa ccattagggc aatatgcaag
     9421 actagattat tcatgacaaa atcagaaagt ttcaacttag catcccgaca ttcatccaag
     9481 acaataatca tgatttctgc aaaattaata ccttcaagaa aggtattacc taccatggtg
     9541 gacaaggtgt tatcaaaact agtaacaaaa aaataatcca tgataaaatg cctaatgtcc
     9601 tcttctttac ctcttatttg aatatgttgg tgagttattt caagctctaa ctggtaaggt
     9661 attatgcgct ccttaatgcc tgctaaaaca tgttttaaag ttgtccgact gatatacaat
     9721 tcttgacaaa gctcttcaaa atcctgaacg gcatcttcaa aaaagaattt atgaagcaca
     9781 taatcttctc gatcaaccga ttcttccact tgagtcacat ctgctaaacg ttttttagac
     9841 gtcacacttt cttgccaaaa gatgtcaaat gccatagagt ggtcaatttc taagcagtaa
     9901 cctcgacctt gtttagaaat gatgtgagct ccgtgttcag gaaggctact gatcagttct
     9961 ttgaggtatt tcctaaccgt acggtcactc ataccaataa cctcagccag agttgcacta
    10021 gagacaaact gctctttgtg atggattaga aatgttatga tggccagttc tttttttgat
    10081 aacattagtc ctccttacta ctttagaaaa cgctctctac cctcttttag tgtaccaaat
    10141 atttcctgaa aaaggtgacc atcattttcc gcattgattg tgaaaagaat ctatgatggg
    10201 tcaatctcca aacaaaattc ctgccaaggt cgcaaatcat ctaacaaact aagatagtcc
    10261 tctctgacct cagcaaccac attagctttt gaactaaccg taaaattttt caaagcaatt
    10321 tgcagttctc ccttatatcg atgatagcgg tcattgtcaa taataatgct acctcgtttg
    10381 acctccttag attggtctct tggagcaatc gattcctgag cataaactag acgtggcatg
    10441 gttgatcgaa tcacataatc agatacatcg ccacgataac aatgcggata gccaatgatt
    10501 ttctcctcaa cttctgtcaa atcgatgata ggcctaacct tgatcgttgt gactggtctt
    10561 gctaaagcct gagtacatgc tgccaattct tcttccgaaa tgaattggtt agatatgagg
    10621 atattatcaa tggttcctat cgctttcatt aattctactt gcaggccaat gggcaagtgc
    10681 ctatggtctt ccactgtcgg caagccttct gccagtagcc aagggccttc agatgctgac
    10741 tgagcggtaa taaaagcagc cgtttcaata tcatgctcat gataaaaacg tgacatatcc
    10801 ttaaaatgtt gccatgacaa accggtaaat tcatgtggat aaaaattatg gcaaccaata
    10861 atattgctct gttctgcatc tgtcgctagc aatgacatca ataattgttt atcagtgctc
    10921 atattaagtt caattttaag cccaaaggga tttcttgtta aagttacaat ctctgctaga
    10981 gggagagcct cgtctaagcg taagcccgct aacccgaagg catgagctcg ttcaataagc
    11041 tgatctgacc aaccagcttg gctaataaag cttggactaa cgtcagcaat tactcgaatg
    11101 cctaattgat ttgcataagc aatgagctct gcgtagcaat gaaacatctg gtgatcagct
    11161 ggtgccagct gtaagagact cataaacaag cgcttggctc catagctatg acataagtca
    11221 atataagctt tactcttggt aacatcatac cgctcagggt atagagaaaa tcctaaatca
    11281 accatattgc ctcctattta tcaaaaccat ttgactcgct caacgcctta aaccagtaac
    11341 ctgatttttt tatggttcgc ttttgactct ctaaatccaa agctaccaaa ccatatcggt
    11401 ttttataagc atttaaccaa gaccagcaat caataaaagt ccacaagagg tagcctttgc
    11461 agttggctcc ttctcccaaa gccttatgaa gctggataag atgatcctcg ataaaggtga
    11521 tgcggtagtc atcttgtatc tggccatctg ctaaaaaagc ttcttcacct tcgacaccca
    11581 taccattttc cgtgactagc cattcgatgt taccatagtg ttcttttaaa ttaagggcaa
    11641 tatcataaag tcctggttca taaatttccc aaccccggtg aggattgact tttttaccag
    11701 gcatatcata aggttcaaaa taaatatcta agataagggg gtccccctct tgctgtgact
    11761 tactaggtgc ctgaacccgc aaaggctgat aataattaac tcccaaaaaa tcaacagtat
    11821 tgtttttgat aacctctaat tcatcagcac tatattgagg taataaatca gcttcctcta
    11881 aaatggaaac taactctgct gggtaaacac ccaaaactga tggatctagg aaggatttgg
    11941 tttggaaaag ctctgcgatt ctagcagcct tgacatcttc aggagcatca ctccgaggat
    12001 aagcaggtgt catgttaagc acaatactaa tcttgtggtc gggatgaagc ttatgacaag
    12061 ctttaaccgc cagtgaactg gccagttgtg tgttgtaagc cacttgaact gctgccttgg
    12121 catctacttt acaagggtag tgataataac ctaaatagcc acattcgact ggaacaatcg
    12181 gttcattaaa ggtaatccaa gtgtttacta aatcaccaaa aagctcaaaa caggtttttg
    12241 cgtaggtttc ataagcccag acagtagctt tgttttccca gccacctttt tcttgcaagg
    12301 cataggggag gtcaaaatga tagaggttaa caatcaattt aataccttga gcaataatgt
    12361 cttgaaatac ttctcggtaa aatgtcaccg cctttggatt aacctccccg actccttctg
    12421 gaatcaatcg agaccactgg attgaggtcc gaaaaatcgt gtggcctgtt tctttcaaaa
    12481 gggcaatgtc acccttatag ttttcataaa aggttgaggt tttttctgga ccaatttggt
    12541 gatgaaactt agctgactca atactaaacc agtaatccca gttgctaggt cctttgccat
    12601 ctccaggaac cgttccttcg ctttgaggcc cagatgtgga acttccccat aaaaagccat
    12661 ctggaaattg ataacggtgt tttctttgtt tcatatcctt tgtcctttct ttgctcactt
    12721 ctctttttat tataagaaga aaaacaggtg atgtaaacgc tttaaaaaat ctgttttttc
    12781 cttatcatca cggaaaataa gtgatttaaa gcttgacata ttttattatc ttaaagagcc
    12841 tatctaacct gccctgctac ttcttatcaa aaccaagccc ctattgacag atgccttagc
    12901 ctatgtttga ataggggtat ggcttattta aactacttta caaaaaccga gtgggcactt
    12961 tggttaagct ctgtcttggc cattttaatt agcgcccttt tattcggtaa gcaagctccc
    13021 ttagctttaa tagcttcttt gattggtgtg acctctctca tttttagtgc taaagctaat
    13081 cctattggtc aagggctcgt cattattttt tccattatct atgcctatct ttcattacga
    13141 aacagttact atggagagtt aatgacctat cttttcatga ccctccctat gactattttt
    13201 tcacttttta cttggttaaa ccatcctttt gaagggaaaa aatcacaagt gactatttca
    13261 cgattaacac ccactgatcg aagatgtctt ttcgttttta ctatactggt cactcttatt
    13321 ttctacggca tcttggcttc tattcaaaca gcctatttac ttgtctccac tttatcgatt
    13381 gccacttctt tctcagccgt ttatctcagc tataagcgaa gcccttattt tgctttggct
    13441 tatggactca atgatctagt cctcattctc ttatggatgc atgctgctca aacagatgcc
    13501 agccaatatg ctgtggtcat ctgctttgcc acctttctta ttaatgatag ttacactttt
    13561 tatagttggc taatgctcca acgttaccaa gaaaaaaagg tcaaaaaagg atgagggcct
    13621 aagcctcatc ctttttcaat ttttcacgtt ctaagcgcaa acgtctattg ggatttaaaa
    13681 gattaaacaa ttcttctaat ttctcagcat tccaaacatc tgctgccgat acaaaatttc
    13741 catccttatc cttaaaggat atcggtttat cacccataat cagaaacctc catcttaaag
    13801 ataaaacaca aggctgctat ggcaatgcca tttctaaaga ccacattaat ccacaaattc
    13861 aaattcaaat ttgccaatac gaacaagatc cccgtctttt gctccacgtt cacgaagggc
    13921 ttcgtcaacc cccataccgc gcaattgacg ggcaaatttc atgatggatt cgtcacgttc
    13981 catattggtc atgacaaaga gacgttctaa cttctcacca gacaagaccc aagtagcgtc
    14041 atcgtcacgt gtgatctcaa attctttctc agcctcagca aaaccatagt aagcttcctc
    14101 gtccaccaga tcagattcat catacaataa gaattcatcc gtcttagcaa gtaattctgc
    14161 cgttgcttct aacaagttct ctaagccttg atgtgctagg cttgaaatag ggaaaatcat
    14221 tggcaaatca tcaaactcgt catattgggc agccaatttt ttcttgaagg ctttcaagtt
    14281 ttcttgagcc tctggcatgt ccattttatt agccacaata atctgtgggc gctccatcaa
    14341 tcgcaaatta taggtttcca gctcgttatt aatagaaaca taatcttcgt aaggatcacg
    14401 accctcacta gcagacatgt caatcacatg aagaataacc cgtgtccgct cgatatgacg
    14461 gaggaattga gttcccaaac caaccccttg gctagcacct tcaatcaaac ctggcagatc
    14521 tgccatagca aagctatcac ctgactttgt acgaaccatt ccaagattag gaacaatagt
    14581 ggtaaaatga taggcaccaa ttttcggttt agctgatgac acaacactta acaaggttga
    14641 tttcccaaca gatgggaatc cgaccaagcc aacgtcagct aagattttga gttctaactc
    14701 taactgacgt tcctcacctg gctcaccatt ttcagcaatc tctggcgcag gattacgagg
    14761 agttgcaaaa cggatattcc cacggccacc acgaccaccc ttagcaatga ccacttcttg
    14821 accatgttct actaagtctg taattacttt tccagtttca gcatcacgaa ccgttgtccc
    14881 ttgaggaaca aaaacaatca aatcttctgc accacggccg tgcatgcctt tggtcatacc
    14941 tttttcacca gatttcgcct tgaatttacg attatagcgg aaatccatca aggtacgcaa
    15001 accttcgttc actcggaaga taactgagcc acctttaccg ccgtctcctc cccaaggacc
    15061 gccatttggg acgtactttt cacgacgaaa agctaccata ccatcgccac cacggcctgc
    15121 ttggacactg atttttgccg tatctaaaaa catactcata ctttttttat tctctaactt
    15181 tcttttactt taaaaaaacg cctttcagcg tttccttaca ttaatactga tagggcactc
    15241 gcaataagcc cgccaacagt tacaagaacc attaggataa caacgaccat tgtcactttt
    15301 tcaaaggttg tttttttgcg aggtccattt tctccaaatg ccacgttaaa tacctcatct
    15361 attgattttt cttcttatat cttaacatga aagatcgttt taggcaaata ccagatgaat
    15421 atttacgcta tgtaggctat tactgagtat tgtatcttgt gaatttcata tgaatatgat
    15481 acattagcat ttgcaaaata atgttacaga taaaaacctc ctcgtctg
//
LOCUS       DATXQK010000038        15346 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.038, whole genome shotgun sequence.
ACCESSION   DATXQK010000038 DATXQK010000000
VERSION     DATXQK010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15346
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.038"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            89..337
                     /locus_tag="VC394_001298"
     CDS             89..337
                     /locus_tag="VC394_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEP5994767.1"
                     /translation="MGLIWTLIVGALIGVIAGALTKKGGSMGWIANIAAGLVGSSVGQ
                     ALLGSWGPSLAGMSLIPSVIGAVIVVMITSFVLNKTNN"
     gene            374..985
                     /gene="amaP"
                     /locus_tag="VC394_001299"
     CDS             374..985
                     /gene="amaP"
                     /locus_tag="VC394_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEP5994768.1"
                     /translation="MLSRKEVCMSKLLKISYCLVGLVLLSVFGWVVGITGGYIYLPYS
                     YRWLSWGMDSFPNLLDSALSYYYFWTALVLFVITFLALLVIILYPRIYTEVQLRHKNK
                     KGTLLLKKSAIESYVATAIQTAGLMPNPTVTAKLYKRKFKIIVKGRLASRVAVADQIS
                     GVKEGIEKGLTEFFGINYPVNFKVYVKDIADSDRKHITRNRVE"
     gene            996..1184
                     /locus_tag="VC394_001300"
     CDS             996..1184
                     /locus_tag="VC394_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEP5994769.1"
                     /translation="MEFYEKFKYPIIGGLVGLIIAILLMAFGLFKTLLAIIFIILGIY
                     GGLYAKKTGIIDQFLNRK"
     gene            1197..1736
                     /locus_tag="VC394_001301"
     CDS             1197..1736
                     /locus_tag="VC394_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEP5994770.1"
                     /translation="MTETYIKNTSKDLTSAIRGQLTYDDKVIEKIVGLALENVDGLLG
                     VNGGFFANLKDKLVNTESVRDGVNVEVGKKQVAVDLDIVAEYQKHVPTIYDSIKSIVE
                     EEVKRMTDLDVIEVNVKVVDIKTKEQFEAEKVSLQDKVSDMARSTSEFTSHQVENVKA
                     SVDNGVEKLQDQKAEPRVK"
     gene            1777..1977
                     /locus_tag="VC394_001302"
     CDS             1777..1977
                     /locus_tag="VC394_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEP5994771.1"
                     /translation="MSDEKYNAKLDQAGGKLKEGFGKISGDKSLETEGKVDKVTGKVK
                     EVIADAKDTVKGLAKGLDNKDK"
     gene            1988..2476
                     /locus_tag="VC394_001303"
     CDS             1988..2476
                     /locus_tag="VC394_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEP5994772.1"
                     /translation="MEKTQIKSTLTYDDKVIEKIVGHALENVGGLLAVTGGFFSNIKN
                     NLVNSESVTDGVSVEVGSKEVAVDLAIIVEYGKDIPAIAESIKAIVSQNVDSMTHLKV
                     VEVNVNVVDIRTKEEHEAASVTVQDRVTNAASSTSQFVSEQTEKLKDTISDTVNSDEA
                     AK"
     gene            2639..3280
                     /locus_tag="VC394_001304"
     CDS             2639..3280
                     /locus_tag="VC394_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEP5994773.1"
                     /translation="MISPKKEITSSKYQKIAISVAQRIANGEYEVGEKLKSRTTIAST
                     FNVSPETARKGLNILADLKILTLKHGSGAIVLSKERAIEFINQYESTHSIAVLKEKIR
                     ETINDQGKAMEKMAVLVNDFLMQSQSVSKQYPLAPYEIICSQDSEHFGKSIGVLNIWH
                     QTGATIVAIEHAGQFIVSPGPYSVIEKGDHIYFVGDESVISRMKTFFNLRKGL"
     gene            complement(3423..3965)
                     /locus_tag="VC394_001305"
     CDS             complement(3423..3965)
                     /locus_tag="VC394_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEP5994774.1"
                     /translation="MTKRHTETKAYVKTALTTLLTEQSFETLTVSDLTKKAGINRGTF
                     YLHYTDKFDMMNHFKNDTLDDLYRLLNQAEIYTDTRQVLNQTLSYLIEHREFITALAT
                     ISYLKFPQLIKDFCYQFLTTITGFQDIVTNQYHIPYPYALEVYLASIESIISYWIANG
                     YQETTEEVADIILKAVTLDN"
     gene            4083..4784
                     /locus_tag="VC394_001306"
     CDS             4083..4784
                     /locus_tag="VC394_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5994775.1"
                     /translation="MAFIELKQVSKSYQVGETTVFANHEVSFEINKGELVVILGASGA
                     GKSTVLNILGGMDTVDAGQVIIDGKDIAHYTSKALTQYRRNAIGFVFQFYNLVPNLTA
                     KENVELAVEIVADALDPVTILKEVGLSHRLDHFPAQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDYQTGKQILTLLQDMARTKGTTVVIVTHNAAIAPIADRVIFMHDAQVTK
                     TVINKEPASIETIDY"
     gene            4799..7435
                     /locus_tag="VC394_001307"
     CDS             4799..7435
                     /locus_tag="VC394_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEP5994776.1"
                     /translation="MIKKTLWKDILRAIKNSKGRFISLFFLMALGSFALVGLKVTGPD
                     MERTASRYLERHQVMDLTVLASHQFSQADKQELDTLKGAHLEYGHLLDVSLTSNQKSL
                     RLYSVPKKVSKPVLVKGSWSKRETDLVLSSSLAKNYQIGDELAVTSPMEGLLTTTHFQ
                     VVGFANSSEVWSKSNLGSSSTGDGSLYAYAFVNPNVFKSAFNLLRIRFSNLRLTNAFS
                     KDYQKRVTQNQAHLDNLLKDNGQKRYDDLQNQYDLALKNGRVALAKETVKLAASEENL
                     TFLEGSALQEAKHQIEQGKQALAKEEKQLEQVQATKDKLEKPSYLTYNRSTLPGGEGY
                     HTYATSTTSISNVGNIFPVVLYLVAALVAFTTMTRYVDEERTSSGLLKAIGYSNKDIS
                     LKFLIYGLLASFLGTTLGIIGGTYLLSALISEILTGALTIGKTHLYSYWFYNGIAYLL
                     AMLSAVLPAYLIVKKELFLNAAQLLLPKPPSKGAKIWLEHLTFVWKALSFTHKVTIRN
                     IFRYKQRMLMTIVGVAGSVALLFAGLGIQSSLAKVVEHQFGDLTTYDILAVGSAKATA
                     TEQTDLASYLKQEPITGYQKVSYASLTLPVKGLPDKQSISILSSSATSLSPYFNLLDS
                     QEQKKVPIPTSGVLISEKLASYYKVKPGDQLVLTDRKGQSYKVTIKQVIDMTVGHYLI
                     MSNTYFKNHFKGLEAAPAYLIKVKDKDSKHIKETASDLLTLKAIRAVSQNANHIKSVQ
                     LVVTSLNQVMTLLVFLSILLAIVILYNLTTINIAERIRELSTIKVLGFYDQEVTLYIY
                     RETISLSLVGILLGIYLGKGLHTYIMTMISTGDIQFGVKVDAYVYLVPILVILSLLAV
                     LGIWVNRHLKKVDMLEALKSID"
     gene            7527..8123
                     /locus_tag="VC394_001308"
     CDS             7527..8123
                     /locus_tag="VC394_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEP5994777.1"
                     /translation="MENSIEAYLADSREKVTLGTVVDCFKGKAVSSKVVPGDVGLVNL
                     SDMGTLGIQYHQLRTFQMDRRQLLRYLLEDGDVLIASKGTLKKVCVFHKQNRDVVASS
                     NITVLRPQKLLRGYYIKFFLDSPIGQALLDAADHGKDVINLSTKELLDIPIPVIPLVK
                     QDYLINHYLRGLTDYHRKLNRAEQEWEYIQNEIQKGLG"
     gene            8134..9090
                     /locus_tag="VC394_001309"
     CDS             8134..9090
                     /locus_tag="VC394_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEP5994778.1"
                     /translation="MGSVERTLKMTLATIVAILIAYQLHLDYAMSAGIIALLSVLDTR
                     KSSLVVARNRLLSFFLAFGIAMMCFSLFGFTTVGFMCYLLIIIPLLYHFQIEAGLVPI
                     TVLVTHLIAKKSIALPILSNEFMLFFVGTSVALLFNAYMGPQDQQIRYYHQKVESDLK
                     GILYRFESFLLEGKGQNEGLLIKNLDKILDEALKLVYRERHNQLFQQTNYQVHYFEMR
                     RQQNRLLGQMAINVNTLMRQSKESILLSHLFHETACQLSEQNPALTLIDDIEQLLETF
                     RHGDLPQTREEFERRAVLFQLLQDLERFILLKVEFYQDYQND"
     gene            9187..10527
                     /locus_tag="VC394_001310"
     CDS             9187..10527
                     /locus_tag="VC394_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEP5994779.1"
                     /translation="MNEIKCPHCHTLFTINESEYSQLLEQVRGQAFDEELQKRLINEM
                     ALLEEKAKHQLHEVVAKKETAITSLTNQLEQIEKEQAYLRQEELAKKDQLIASLEAKL
                     DKLASQNALELANQLAEKDKEVVSLTNQLDKLALEKDATFQSKLATIEKERDGIKNQL
                     ALQAKESELSLASVRSDYEAQLKAANERVEFYKNFKAQQSTKAIGESLELYAETEFNK
                     VRSYAFPNASFVKDNQLSSRGSKGDYIYREVDANGVEILSIMFEMKNEADTTKTKHKN
                     SDFFKELDKDRREKDCEYAVLVSMLEADNDYYNTGIVDVSHEYQKMYVVRPQLFIQLI
                     GILRNAALNSLHYKQELALIKEQNIDITHFEEDLDQFKNAFAKNYQSASNNFKKAIDE
                     IDKSIKRMEEVRRFLTTSENQLRLANNKLEDVSVKKLTRQNPTMREKFAALKDQ"
     gene            10643..11527
                     /gene="truB"
                     /locus_tag="VC394_001311"
     CDS             10643..11527
                     /gene="truB"
                     /locus_tag="VC394_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEP5994780.1"
                     /translation="MINGIINLKKEAGMTSHDAVFKLRKLLQEKKIGHGGTLDPDVVG
                     VLPIAVGKATRVIEYMTEAGKVYEGQVTLGYSTTTEDASGEVVARSSLPAVLTEELVD
                     QTMTTFLGKITQTPPMYSAVKVNGRKLYEYARTGESVERPRREVTISLFERTSPLNFT
                     EDGLCRFSFKVACSKGTYVRTLAVDLGRALWVESHMSFLQRSASAGLTLETAYTLGEI
                     ADMVSKQEMSFLLPIEYGVADLPKMVIDDTELTEISFGRRLSLPSQEPLLAAFHGEKV
                     IAILEKRDQEYKPKKVLI"
     gene            11556..12488
                     /locus_tag="VC394_001312"
     CDS             11556..12488
                     /locus_tag="VC394_001312"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEP5994781.1"
                     /translation="MEIEYIKDYRDINQEDDTVLVLGYFDGLHRGHKALFEKAREVAN
                     KEGLKVVVFTFTESPKLAFSRFSPELLLHITYPKKRYEKFADYGVNKLYLVDFTSKFS
                     KVSSDHFITHYIKNLKAKHIVVGFDYKFGHNCTDSDYLTRNFEGQVYTIKEIKEDHRK
                     ISATWIRKLIQEGNVVKANHLLGYDLSTRGRVVHGDARGRTIGFPTANLAPIDNTYLP
                     ADGVYVTNVIVANKIYRSMTSLGKNVTFGGKELRLEVNIFDFDEEIYGEIIEIVWLDK
                     IRDMEKFEGIEDLTDRLEYDKRTALNWKKDSKLS"
     gene            12531..12935
                     /locus_tag="VC394_001313"
     CDS             12531..12935
                     /locus_tag="VC394_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEP5994782.1"
                     /translation="MVTLFLSPSCTSCRKARAWLVKHEVDFQEHNIITSPLSRDELMS
                     ILSFTENGTEDIISTRSKVFQKLDVDVEELSISDLIDLIAKNPSLLRRPIIMDQKRMQ
                     IGFNEDEIRAFLSRDYRKQELRQATIKAEIEG"
     gene            12937..13215
                     /locus_tag="VC394_001314"
     CDS             12937..13215
                     /locus_tag="VC394_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEP5994783.1"
                     /translation="MSGNYYYPLDLSWSTEEISSVLHFLNKVELAYEKKVDAKQLLDS
                     YKTYKTIVKSKAQEKQIDRDFQKVSGYSTYQVVKKAKAIEKGFFSLGN"
     gene            13205..13993
                     /locus_tag="VC394_001315"
     CDS             13205..13993
                     /locus_tag="VC394_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEP5994784.1"
                     /translation="METKYAFARQIIKEAGLFIKSKMSEQLDIQVKTQFDDLVTNVDQ
                     ETQQLLMDRIHQTYPCDAILAEENDVRHPINQGNVWVIDPIDGTVNFIVQGSQFAVMI
                     AYYEQGIGQFGLIYDVMADQLLAGGGDFEVTLNGDKLPAYQEKPLERSLIGCNAGMFA
                     RNDRNLAHLIAKTLGVRVYGGAGICMVKVMKQELLAYFSFIQPWDYAAAKVLGDKLGY
                     VLLTIDGYEPDFQTRQKIMFVPKCQLTRIASFLTKDSAVTLEDQ"
     gene            13996..15306
                     /locus_tag="VC394_001316"
     CDS             13996..15306
                     /locus_tag="VC394_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEP5994785.1"
                     /translation="MMSLPKEFINTYQAILGKELEDFLASFNQEPVNAFRINPLKNQL
                     KTFEHPIPNTLWGYYGKLSGKSPEHVSGLVYSQEPAAQMVAQVAAPQKGSRVLDLAAA
                     PGGKSTHLLAYLDNTGLLVSNEISKKRSKVLVENIERFGARNVVVTNESADRLAKVFS
                     HYFDTIVFDGPCSGEGMFRKDPDAIQYWHHGYPAECAKLQKSILEDALTMLKPGGELI
                     YSTCTWAPEENEDVVQWLLETYTFLELVDVPKLNGMVSGIGLPETARMYPHRYQGEGQ
                     FVAKLKDKRQEGQSTKLKAPKSNLTKDQLRLWKMFEKDHLNLTLSGTLQTFGDYLYLL
                     PDGLPALDGLKIARNGLELGVFKKKRFEPSYALGLALKPDEVRSSIELREEDFRPYVS
                     GNVITLPQSYPTGWYQLLINGNGLGFAKVVDKTIKNNFPKGLRF"
BASE COUNT         4937 a         2534 c         3189 g         4686 t
ORIGIN      
        1 gactattaaa agactattgc ttgaagaata acagctgtta ttcttccttg gacaaaaggt
       61 acaggagtat ctaaaaagga gaattatcat gggacttatt tggactttaa tcgtcggtgc
      121 gcttattggt gtgattgctg gagcacttac taaaaaaggc ggttcaatgg gctggattgc
      181 aaacatcgct gctggtctag ttggttcttc cgttggtcaa gctttacttg gttcatgggg
      241 cccttcttta gcaggaatgt ctttgatccc gtcagttatt ggtgccgtta tcgttgttat
      301 gatcacttct tttgtactaa acaaaactaa caactagttt cctctttatt tagtaagtac
      361 ttacaaatag agaatgttaa gtagaaaaga ggtctgtatg tcaaaattgt taaagattag
      421 ttattgtctt gtcggacttg ttctattgtc tgtttttgga tgggtagttg gaataaccgg
      481 aggctatata tatttacctt atagttatcg ttggctaagt tggggtatgg atagttttcc
      541 caatctctta gattcagctt tatcctatta ttacttctgg acagcccttg tattatttgt
      601 tataacattt cttgccttat tagttattat tttataccca agaatttata ctgaggtcca
      661 gttacgccat aaaaataaaa aaggaacact tttattaaaa aaatcggcta ttgaaagtta
      721 tgttgcgacc gctattcaaa ctgcaggttt gatgccaaac ccaacagtca cagctaaatt
      781 atataagcgg aagtttaaga ttatcgttaa aggacgttta gcttctcgtg tagcagtggc
      841 agatcaaatt agtggcgtga aagaagggat cgaaaaaggt ttaaccgagt tcttcggtat
      901 taattatcct gttaatttca aagtttatgt taaagatatt gctgattctg atcgtaaaca
      961 tattaccaga aatcgtgtag aataggagat ctatcatgga attttatgaa aaattcaagt
     1021 accctattat tggtggcttg gtggggctaa ttattgccat attgctcatg gcttttggtt
     1081 tattcaaaac gctactagca attattttca ttattttagg aatatatggt ggcctgtatg
     1141 ccaaaaaaac aggtattatt gatcaatttc ttaatcgcaa ataaaggaga cttattatga
     1201 ctgaaactta tattaaaaac acatctaaag acttaacttc agcaattcgt ggtcaattaa
     1261 catatgatga taaagtaatt gaaaaaattg ttggtttagc acttgaaaat gttgatgggt
     1321 tactgggtgt aaatggtggt ttttttgcta acctaaaaga taaattagta aacaccgaat
     1381 ctgtacgtga tggcgtgaac gtggaagttg gaaaaaaaca agttgccgtt gatcttgata
     1441 ttgtcgctga atatcaaaag catgtgccga ctatttatga ctctattaaa tcgatcgttg
     1501 aagaagaagt gaagagaatg accgatctgg atgtgattga agttaatgtt aaagtagtcg
     1561 acattaaaac aaaagaacaa tttgaagctg aaaaagtaag tttacaagac aaagtttcag
     1621 acatggcacg ttcaacatct gaatttacga gtcatcaagt tgagaatgtt aaagcttcag
     1681 tggataacgg tgttgaaaaa cttcaagatc aaaaagctga gccacgtgta aaataatcat
     1741 atgtcgtcaa ttaggtaagc taaaggaggg tttattgtgt cagatgaaaa atataatgcg
     1801 aaattagatc aagctggtgg caagttaaaa gaaggatttg gtaagatttc tggagataaa
     1861 tctttagaaa ctgaaggtaa agtagataaa gttactggaa aagttaaaga ggttattgca
     1921 gacgccaaag atactgtaaa aggattagct aaaggcttag ataataaaga taaataaagg
     1981 ggacatgatg gaaaaaactc aaattaaaag cactcttact tatgacgata aggtcatcga
     2041 aaaaattgtc ggtcatgctt tggaaaacgt tggcggatta ctagctgtaa caggtggatt
     2101 tttctcaaat attaaaaata atttggtgaa ttctgagtct gtaactgatg gtgtttcagt
     2161 tgaagttggt agcaaggaag tagcagttga tttagcaatc attgtagaat acggtaaaga
     2221 tattcctgct attgcagaat ctattaaagc tattgtttct caaaacgttg atagtatgac
     2281 acatttgaaa gttgttgaag tcaatgtgaa cgttgttgat attcgtacta aggaagaaca
     2341 tgaagctgct agcgttactg ttcaagatcg tgtcactaat gcagcttcaa gtacttcaca
     2401 atttgtatca gaacaaacgg aaaaattaaa agatactatc tctgatactg taaatagtga
     2461 tgaggcagct aaataagcaa tcgtctagga agtattattg actagtggtg ataagtttga
     2521 gctggtattt accagctctt tttgtcacgg ttaaaaagac acggactatt gtcatctctc
     2581 taaaaatatg atttaattag atagaaattt ttcaaattgt tagaaaggag taaattaagt
     2641 gataagtcct aaaaaagaga taacaagttc aaaatatcaa aaaattgcta tttcagtagc
     2701 tcagcgtatc gcaaatggcg aatatgaagt gggcgaaaag ttaaagtcaa gaactactat
     2761 cgcctcgaca tttaatgtat caccagaaac ggctcgtaaa ggcctgaata ttttagctga
     2821 cttgaaaatt ttgactttaa aacatggtag tggagctatt gtgctgtcaa aagaaagagc
     2881 tattgagttt ataaatcaat acgaatcaac ccattccatc gctgttttaa aagaaaaaat
     2941 acgtgagact attaatgatc agggaaaagc aatggaaaaa atggctgttt tggtcaatga
     3001 ttttttaatg caaagtcaat ctgttagtaa gcaataccca ttagcacctt atgagattat
     3061 ttgtagtcaa gattcggaac attttggcaa atctattggt gtgttaaata tctggcacca
     3121 gacaggagca actatcgtcg ctattgaaca tgctggtcag tttatcgttt caccaggtcc
     3181 ttattctgtt attgaaaaag gcgatcatat ttatttcgtt ggtgatgagt cagtcatttc
     3241 acgaatgaag acctttttta atttacgaaa aggattataa gtttgagggc ccacctcaaa
     3301 ctttttttgt tgctgttgag cattattaaa agaggcagat catcatggtt tgtctttcca
     3361 aaatatctgt ttagacagtt gttttggatt gggagcatga tttcagactc ttttgatgtt
     3421 ttttaattgt ctagagtaac tgctttaaga ataatatcgg caacctcttc agtggtttct
     3481 tgatagccat tagcaatcca atagctgata atgctctcaa tactggcaag gtaaacttct
     3541 aaagcatagg gataaggaat atggtattga ttggtcacaa tgtcttggaa accagtaata
     3601 gtagtcaaaa attggtaaca gaaatccttg atgagttgcg gaaatttgag gtaagaaata
     3661 gtagcgagtg cggttataaa ttctctatgt tctataaggt aggacaaggt ttgattgagg
     3721 acttgtcgag tgtctgtgta gatttcagcc tgatttaaca aacggtacaa atcatccaga
     3781 gtgtcatttt tgaaatgatt catcatgtca aatttatcag tgtagtggag gtagaaggtt
     3841 cctctattga taccagcctt tttagtaagg tcagagacag taagagtttc aaaagattgt
     3901 tcagttagta aggtggttaa ggcggtcttt acataagctt tggtttcagt atgtcttttg
     3961 gtcattctca ggtgctcctt aaatcaacag ttgcgtcaga tttgttcttg ttaaatactt
     4021 atggccatta tataataagg ctataattaa atcaacacta tgttcaaata atggaggggc
     4081 agatggcttt tattgaatta aaacaggttt caaaatctta tcaggtagga gagacgactg
     4141 tttttgccaa ccacgaagtg tcttttgaga ttaataaagg agaactagtg gttattttgg
     4201 gagcatccgg agctggtaaa tcgacagtcc tcaatatttt ggggggcatg gacacagtag
     4261 atgcaggtca agtaattatt gatggcaaag acattgctca ttacacgtct aaggccttaa
     4321 ctcagtatcg tcggaatgct attggtttcg tttttcaatt ttataatttg gttcctaatt
     4381 tgacagctaa agaaaatgtt gaattagcag ttgaaattgt agcagatgct ttagatcctg
     4441 tgaccatttt aaaggaagta ggactcagtc atcgtctgga tcattttcct gctcagctct
     4501 caggtggtga acagcaacgg gtttcgatag cacgtgcctt agctaaaaac cctaaattgc
     4561 ttctttgtga tgaacctaca ggtgcccttg actaccaaac aggaaagcaa attctaaccc
     4621 tcttacagga tatggcacga actaagggga ccacggtagt tattgtaacc cacaatgctg
     4681 ctattgctcc gatagctgat cgtgtcattt ttatgcatga tgcacaggtt accaagacag
     4741 tcattaataa ggaacctgca agcattgaga caattgatta ttaagaggaa ggtgtcgtat
     4801 gataaagaaa accttatgga aggatatttt gagagctatc aagaatagta aagggcgatt
     4861 catttcctta tttttcctaa tggccttagg ctcctttgct ttagttggac ttaaagtcac
     4921 cggtccagat atggaacgaa cagccagccg ttacctcgaa agacaccagg tgatggacct
     4981 aacggtactg gcttctcatc aattttccca agccgataaa caagagttag atacgttaaa
     5041 aggggctcat ttagaatatg gtcatttact tgatgtcagt ctaactagca accagaaatc
     5101 tcttaggctc tatagcgtgc caaagaaagt gtctaagcca gtcttggtta aagggagttg
     5161 gtcaaagcga gagacagatt tggttttatc ctcctcactt gctaaaaact atcagattgg
     5221 agatgaacta gcagtcacct cacctatgga aggtttgctg acgacaaccc attttcaagt
     5281 ggttggtttt gcgaattctt cagaagtttg gtccaagtcc aatttaggaa gttcctcaac
     5341 cggagatgga agtctctacg cttatgcttt tgttaatcca aacgttttta aaagtgcctt
     5401 taacctgctg cgcatccgtt ttagtaatct tcgtctgaca aacgcttttt ccaaagacta
     5461 tcaaaaaagg gtaacccaaa accaagctca tttggataat ctacttaaag ataatggcca
     5521 gaagcgttat gatgacctcc aaaatcagta tgaccttgcc ttaaaaaatg gcagagtagc
     5581 acttgcaaag gaaacagtga aactagctgc gagtgaggag aacttaactt ttttagaagg
     5641 ctctgcttta caagaagcta agcatcagat tgaacaaggc aaacaagcat tagccaagga
     5701 ggaaaagcag ttagagcagg tgcaggctac aaaagataag ctagaaaaac ccagttacct
     5761 gacttataat cgctcgaccc taccaggagg agaaggatat catacttatg caacttcaac
     5821 gacctccatt tcaaatgttg gaaatatttt tcctgttgtt ctttatctcg tagctgctct
     5881 agtagccttt accaccatga cacgttatgt tgatgaagaa agaacaagct ctggtctatt
     5941 aaaagccatt ggttattcta acaaggatat cagtttaaag tttcttatct atgggctttt
     6001 agccagtttt ttagggacaa ctttaggtat tattggggga acttacctct tatctgcctt
     6061 gatttcagag atcttaacag gagctttgac tattggaaag actcaccttt atagttattg
     6121 gttttataat ggcatagctt acttgctggc tatgttatct gctgttttac ccgcctactt
     6181 aattgtcaaa aaggaattat tcctcaatgc agctcagtta ttgctgccca aacctcctag
     6241 taagggggca aaaatctggt tggaacacct tacttttgtc tggaaagccc tgtcctttac
     6301 tcacaaggtg accatacgta atatttttcg ctataaacaa agaatgctga tgaccattgt
     6361 aggcgttgca ggctcagtag cccttttatt tgcaggctta gggattcagt cgtcattagc
     6421 caaagtagtt gagcatcaat ttggtgattt aacgacttat gatattttgg ctgtcggttc
     6481 ggccaaagcg acagcgacag agcaaactga cttagctagc tatcttaaac aagaacctat
     6541 tacagggtac caaaaggtat cttatgccag cttaaccctt cctgtaaagg gattacctga
     6601 taagcaaagt atttccattt tatcaagttc agctacttct cttagcccct attttaatct
     6661 gctggatagt caggagcaaa agaaggttcc cattccaacc tctggtgttt tgatttctga
     6721 gaaattagcc tcctattaca aggtaaaacc aggtgatcag ttggtattga ctgatcggaa
     6781 aggacagtct tataaagtga cgattaaaca ggttattgac atgacagttg gccattacct
     6841 gataatgtct aatacctatt ttaagaatca ttttaaagga ttggaggctg ctcctgccta
     6901 tctgattaag gtaaaagaca aagatagcaa gcacataaag gagacagcca gtgacttgtt
     6961 aaccttaaaa gcgattagag cagtttcaca aaacgccaat catattaaat ctgttcagct
     7021 agtagtcacc tctcttaatc aggtcatgac cctccttgtc ttcttgtcta ttttattagc
     7081 aatcgttatc ctttataact taacgactat taatattgct gagcgtattc gagaattatc
     7141 cactattaaa gttctgggat tttacgatca ggaggtcacc ttatatattt atcgagaaac
     7201 tatttcgcta tccctagtag gcattctttt aggtatctat ttaggaaaag gcctgcatac
     7261 ttatatcatg acaatgattt caactgggga tattcaattt ggtgtaaagg ttgatgctta
     7321 tgtttatcta gtgccaattc tagtaatcct tagcttgtta gcggtattag gtatctgggt
     7381 taatcgccat ttaaaaaagg ttgatatgtt agaagctttg aaatccatag attgattggt
     7441 gaaaagacag atatagacag cacttgaaaa gttaatcata ttatgatagt atctaaatac
     7501 tataatttaa tagaaagata ctatgtatgg aaaatagcat tgaggcctat ctagcagata
     7561 gcagagaaaa ggtaacatta ggtacagttg ttgattgttt taaaggaaaa gcagtctcaa
     7621 gtaaggttgt gccaggtgat gtgggattgg ttaatttatc tgacatgggc accttaggga
     7681 ttcaatatca ccaattaaga actttccaga tggatcgtag gcagctcttg cgttaccttt
     7741 tggaagacgg agatgtctta atcgcctcta agggaaccct aaaaaaagtc tgtgtttttc
     7801 ataagcaaaa tcgagatgtg gtggcatcat caaatatcac tgtcttacgc cctcaaaaac
     7861 tgttaagggg ctactatatc aagttctttt tggactcacc tattggtcaa gcgctcttgg
     7921 acgcagcgga ccatggcaaa gatgtgatca atctctcaac gaaagaattg ttggatattc
     7981 caattcccgt tattccttta gtgaaacagg actatttgat taatcactac ttgagaggat
     8041 taacagatta tcatcgtaaa ctcaatcgag cagaacagga gtgggaatat attcagaatg
     8101 aaattcaaaa aggattaggt taggagtctt gtcatgggtt cagttgaacg tacattaaaa
     8161 atgaccttgg ctaccattgt tgccatttta attgcttacc aacttcatct ggattatgca
     8221 atgtcagcgg ggatcattgc cctgttaagt gttttagaca cccgtaagtc cagtttggtg
     8281 gtcgctagga atcgcttgct atcttttttc ctggcttttg ggattgcgat gatgtgcttt
     8341 agcttgtttg gttttacgac agtaggtttt atgtgttatt tgttaattat tattcctttg
     8401 ctctatcact ttcagataga ggcaggactg gttcctataa cagtacttgt gacacatttg
     8461 attgctaaaa agagtattgc attacctatt ttatcgaatg agtttatgct cttttttgta
     8521 gggactagtg tcgctttact atttaatgct tatatgggtc cacaagatca gcaaattcgg
     8581 tactatcacc aaaaagttga atcggatctg aaaggtatct tataccgttt tgaaagtttt
     8641 ctactggaag gaaaaggtca aaatgaaggg ctcttgatca aaaaccttga taaaatatta
     8701 gatgaagctc taaaactagt ttatcgagaa aggcataatc aactatttca gcagaccaat
     8761 tatcaagttc attattttga gatgcgacga caacaaaata gactattggg acaaatggct
     8821 atcaacgtaa acacgttaat gagacaaagt aaggaaagta ttcttttgtc acatcttttt
     8881 cacgaaactg cttgtcagct aagcgaacaa aatccggctt taaccttgat tgatgatatc
     8941 gaacaattgc ttgaaacctt tcgtcatggt gatcttcctc aaactcggga ggaatttgag
     9001 cgacgtgcgg ttttatttca gctcttacaa gacttggaac gttttatctt attaaaggta
     9061 gagttttatc aggattatca aaatgactaa atctctattg cacacttctt atctatgaca
     9121 gcttgggact taacttcctg agcttttttg ctataataaa aacaagaatc tcagaatgga
     9181 gacaagatga acgaaataaa atgccctcat tgtcataccc tatttactat caatgagtca
     9241 gagtatagtc aattattgga acaagtcaga gggcaagcct ttgatgaaga attgcaaaaa
     9301 cggttaatca atgagatggc cttgctggaa gaaaaagcga agcatcaatt gcatgaggtt
     9361 gtcgcaaaaa aagaaacagc tattaccagc ttgacaaacc aattagagca aattgaaaaa
     9421 gaacaagcct acctcagaca agaagaactg gctaaaaaag accagcttat tgcttcttta
     9481 gaagcgaaac ttgataaatt agcctcacaa aatgctttgg aactggccaa tcaattagct
     9541 gagaaggaca aagaagtggt tagtttgacc aatcagctgg acaaattagc acttgaaaaa
     9601 gatgccacgt tccagtcaaa gcttgctaca atcgaaaaag aacgcgatgg aatcaaaaac
     9661 caattggcgc ttcaagctaa agaaagtgaa ttgtcgttgg cttcggtgcg cagcgactac
     9721 gaagcacagt taaaagcggc taacgagcga gttgaatttt ataaaaattt caaagcgcag
     9781 caatctacca aggctatcgg agagagtcta gaactctatg ctgaaacaga gtttaacaaa
     9841 gtaagaagct atgcttttcc caatgctagc tttgtcaaag ataaccaatt gtctagtcgt
     9901 gggtcaaaag gggactatat ttaccgagag gttgatgcca atggtgtaga aatcctctct
     9961 atcatgtttg aaatgaagaa cgaggcagac accaccaaaa caaaacataa aaatagtgat
    10021 tttttcaagg agttagataa agaccgtcgc gaaaaagatt gtgagtatgc tgttttggta
    10081 agcatgttag aagctgataa tgattactac aatacaggga ttgttgatgt cagtcatgag
    10141 tatcaaaaga tgtatgtggt tcgtcctcaa ctctttattc agttgatagg gattctgcga
    10201 aatgcagccc tgaatagttt acattataag caagagttgg ccttgattaa ggaacaaaat
    10261 attgacatca ctcacttcga agaggatttg gatcaattta agaatgcctt tgctaagaac
    10321 tatcagtctg ccagcaacaa ctttaaaaag gctattgacg agattgataa atcgattaaa
    10381 cgcatggagg aagtgagacg ctttttaacc actagtgaaa atcagttacg cttagctaat
    10441 aataaattag aggatgtttc agttaaaaaa ctgacccgcc aaaacccaac catgcgagag
    10501 aaatttgcag ccctcaagga ccagtaagcc tgaaagtgag tctttccctg ctaaaattgg
    10561 ttaagcgttt attttggcaa aactgatgat aaagcggtag aatagaatca gtaacaatta
    10621 ctaattagag agaaaaatag acatgataaa tggaatcatt aatttaaaaa aagaagccgg
    10681 catgacctct catgacgcag tttttaaatt acggaagtta ttacaagaaa aaaagattgg
    10741 acatgggggg accttagatc ctgatgtggt cggtgttcta ccaattgcag ttggtaaggc
    10801 tactcgtgtg atcgaataca tgacagaagc tggtaaagtt tatgaaggac aagtgactct
    10861 cggttactca acaacgactg aggatgctag tggagaggta gttgcgagat cttctttgcc
    10921 agctgtgcta acagaagaac tcgttgatca aacgatgacg acttttcttg ggaaaatcac
    10981 acagacccca cctatgtatt cagccgtaaa ggttaatggc cgtaaattat acgagtatgc
    11041 gcgtactggt gaaagtgtgg aacgtcctag aagagaagtg accatttctc tgtttgaacg
    11101 caccagccct ttgaatttta ctgaagacgg tctttgccga ttctctttta aagttgcttg
    11161 tagcaagggg acttatgtgc ggaccttagc agttgattta ggtcgagctc tatgggtaga
    11221 gagtcatatg tccttcttgc agcgatcagc ttcagcagga ttaaccctcg agactgccta
    11281 tactttggga gaaattgctg atatggtttc taaacaagag atgtcttttt tgttaccgat
    11341 cgagtatggc gttgctgatc ttcctaaaat ggttattgat gatactgaat tgactgaaat
    11401 ttcatttggt cgccgtctta gtctccctag tcaagagcca ctgttagctg cttttcatgg
    11461 cgaaaaagta attgctattt tagaaaaaag agatcaagaa tacaaaccca aaaaagttct
    11521 tatttaaata gtttgagtaa gtagaatgga taattatgga aattgaatat atcaaagatt
    11581 atcgtgatat taaccaagaa gatgatacag tcttggtatt gggatatttt gatggattac
    11641 accgtggtca taaagcctta tttgaaaaag caagagaggt tgcaaataaa gaagggttaa
    11701 aagtagtcgt ttttacgttt acagagtctc cgaaattggc tttttcgcgt ttttctcctg
    11761 agttattgct tcatattact tatccaaaga agcgttatga aaaatttgcc gattacggtg
    11821 taaataaatt atatttggtt gattttactt ctaaattctc taaggtctca tctgatcatt
    11881 ttataactca ttatattaaa aacctgaaag caaagcatat tgttgtcggt tttgactata
    11941 agtttgggca taattgtaca gatagtgatt atttaaccag aaattttgaa ggacaagttt
    12001 ataccattaa ggaaataaaa gaagatcatc gaaaaatttc tgctacatgg ataaggaagc
    12061 ttattcaaga aggtaatgta gttaaagcaa accacttgtt aggctatgac ttatcgacaa
    12121 gggggagagt cgttcacggg gatgcgcgtg gccgtactat tggtttccca acagccaatc
    12181 tagctccaat tgacaacaca tacttaccag ctgatggagt ctatgttaca aatgttattg
    12241 ttgctaataa aatctatcgc tctatgacga gtttaggtaa aaatgtgaca tttgggggga
    12301 aagagttgcg tctagaagtt aacatttttg actttgacga agagatctat ggagaaatta
    12361 ttgagattgt ttggttagat aagattcgag acatggaaaa attcgaggga atcgaagatt
    12421 tgactgatag attggaatat gataaacgaa cagcattaaa ttggaaaaaa gatagcaaat
    12481 tatcttaaat ttttgtataa taaggtaagt aaatttgata gaggaaaaat atggttacct
    12541 tatttttatc acctagctgt accagttgtc gtaaagcaag ggcatggtta gtaaaacacg
    12601 aagttgactt tcaagagcat aatattatta caagtccatt aagccgtgat gaattaatgt
    12661 caatcctctc ttttacagag aatggaacgg aggatattat ttcaacacgt tcaaaggttt
    12721 ttcaaaaact tgatgttgat gtagaagagc tttcaatttc agatttaatt gatttaattg
    12781 ctaaaaatcc aagtcttctt cgtcgcccta ttatcatgga tcaaaaacgc atgcaaattg
    12841 gttttaatga agatgaaatt agagcgtttt tatcgagaga ttatcgcaag caagaactac
    12901 gtcaagcaac gataaaagca gaaattgagg ggtgatatgt caggtaatta ttattatcca
    12961 cttgacttaa gttggagcac tgaagaaata tcctcagtgc ttcatttttt gaataaagtg
    13021 gagttagcat atgaaaaaaa agtagatgca aaacaactcc tagatagtta taagacttat
    13081 aaaaccattg tcaaaagtaa agctcaggaa aagcaaatcg accgtgattt tcaaaaagtt
    13141 agtggttatt cgacctatca agttgttaaa aaagcgaagg caattgagaa aggattcttt
    13201 tcccttggaa actaaatacg catttgcaag gcaaatcatt aaagaagcag ggctttttat
    13261 caagtcaaaa atgtcagagc agctagatat tcaggtgaaa acacaatttg atgatctggt
    13321 taccaatgtt gatcaagaaa ctcaacagtt actgatggat cgtattcatc agacctatcc
    13381 ttgtgatgcc attttagctg aagaaaatga cgttcgtcac cccattaatc aaggaaatgt
    13441 ttgggtcatc gatcccattg atggaacggt taactttatt gtgcagggat cacagtttgc
    13501 agtgatgatt gcctattatg aacaaggaat tggccaattt gggcttattt atgatgtgat
    13561 ggcagatcaa ttactagctg gtggtggtga ttttgaagtt actttaaacg gtgataagct
    13621 tccagcttac caagaaaaac ctttagagcg tagcttaatt ggctgcaatg caggcatgtt
    13681 tgccagaaat gatcgtaacc ttgctcatct aattgcaaag actttaggtg taagggttta
    13741 tggtggtgca ggtatctgca tggttaaggt catgaagcag gaattactgg cctatttttc
    13801 atttatccaa ccatgggatt acgcagcagc aaaagtacta ggcgataaac tgggttatgt
    13861 tttactaact attgacggat atgagcctga ttttcaaaca cgtcaaaaaa tcatgtttgt
    13921 tcctaaatgc caactcacta ggatagcgtc ttttctaaca aaggactctg ccgtcacact
    13981 tgaagatcaa tagaaatgat gtcattacca aaagaattta ttaacactta tcaagcaata
    14041 ctaggcaaag aattagaaga tttcttggca agttttaacc aagagccggt caatgctttt
    14101 cggattaacc ccttaaagaa tcaactaaaa acctttgaac accccattcc aaatacctta
    14161 tggggatatt atggtaaact atctggcaag tctccagagc atgtgtcggg gttggtttat
    14221 tcccaagaac cagcggctca aatggtggca caagtagcgg ctcctcaaaa ggggagtcgt
    14281 gttttagact tggcagctgc accaggagga aaatcaactc atttattagc ttatttagac
    14341 aatacaggtc ttttagtcag caatgaaatt tctaagaagc gcagcaaggt tttagttgaa
    14401 aatatcgaac gttttggcgc tagaaatgtt gtggtgacta atgaatccgc agatcgttta
    14461 gccaaggtat tttctcatta ttttgatact atcgtctttg atggaccatg ttctggtgaa
    14521 ggaatgtttc gcaaggaccc tgatgctatt caatattggc atcacgggta tcccgcagaa
    14581 tgcgccaagt tgcaaaaatc tattttagaa gacgccttaa ccatgttaaa accaggagga
    14641 gaattgatat actccacctg tacttgggct cctgaagaaa atgaagatgt tgtgcagtgg
    14701 ctcttagaga cttatacctt tttagaattg gtcgatgttc caaaactaaa tggaatggtt
    14761 agtggtatcg gacttccaga gactgctaga atgtaccctc atcgttatca aggggaaggg
    14821 caatttgtgg ccaagctaaa agataagcgt caagaaggac aaagcactaa actaaaagca
    14881 cctaaatcca acttgaccaa agaccagtta cgtctttgga aaatgtttga aaaagaccat
    14941 ctaaacctta ccctatcagg aacactacaa acctttggtg attacctata cctcttgcca
    15001 gatggtcttc ctgctttaga tggccttaaa attgctcgga acggtttaga attaggcgtc
    15061 ttcaagaaga aacgctttga accctcttac gccttgggat tagctttaaa gcctgatgag
    15121 gtccgatcat ctattgagct tagagaggaa gactttagac cttatgtgtc aggtaacgtc
    15181 attactttgc cccaatctta tccaacaggc tggtaccaat tactcattaa tggcaatggt
    15241 ctaggatttg ccaaggtcgt tgacaagacc atcaaaaata atttccctaa agggttaaga
    15301 ttttagcgta aaaaataata ttaaaaagtt aaatatttaa agaaaa
//
LOCUS       DATXQK010000039        15050 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.039, whole genome shotgun sequence.
ACCESSION   DATXQK010000039 DATXQK010000000
VERSION     DATXQK010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15050
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.039"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            5..78
                     /locus_tag="VC394_001317"
     tRNA            5..78
                     /locus_tag="VC394_001317"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Asn,seq:gtt)
     gene            158..231
                     /locus_tag="VC394_001318"
     tRNA            158..231
                     /locus_tag="VC394_001318"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:192..194,aa:Arg,seq:acg)
     gene            227..808
                     /locus_tag="VC394_001319"
     CDS             227..808
                     /locus_tag="VC394_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEP5994786.1"
                     /translation="MHNEKSNRTLVSCCFFVVLFILGIVKKKRRLKMSIETRAAFEKV
                     KPIILKLKRHYYIQLWDRDDWLQEGHIILLQLLERYPELIEEEERLYRYFKTKFSSYL
                     KDLLCRQESQKRQFHKLAYEEIGEVAHAIPSRGLWLDDYVAYQEVIASLENQLNSQER
                     MQFQALIRGERFKGRRALLRKISPYFKEFAQQL"
     gene            993..1742
                     /gene="truA"
                     /locus_tag="VC394_001320"
     CDS             993..1742
                     /gene="truA"
                     /locus_tag="VC394_001320"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEP5994787.1"
                     /translation="MVRYKATISYDGTLFSGFQRQRHLRTVQEEIEKTLYKLNNGTKI
                     IIHGAGRTDAGVHAYGQVIHFDLPQEQEVEKLRFALDTQTPEDIDVVNIEKVADDFHC
                     RYQKHLKTYEFLVDNGRPKNPMMRHYTTHYPYTLNIKLMQEAINGLVGTHDFTGFTAA
                     GTSVQNKVRTITKATVSRDEKTDFLVFTFSGNGFLYKQVRNMVGTLLKIGNGQMPVEQ
                     VKVILSSKNRQLAGPTISGNGLYLKEICYEN"
     gene            1732..2496
                     /locus_tag="VC394_001321"
     CDS             1732..2496
                     /locus_tag="VC394_001321"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEP5994788.1"
                     /translation="MKTDYIVTISGNDILSGGGLYADLATYIRYDLQAFVAVTCLTTR
                     SEEGFSLFPVAKEIFRDQLNSFTNAPISAIKIGLLPNAEMCEIVLDFIKGHLGIPVVL
                     DPVLACKEIDDVKIVPLRQEILQLLPYVTVVTPNLVEAQLLSQKEIVSLKDMQEAAKY
                     FYQLGAKQVVIKGGNRFSQKKAIDLFYDGKEIVTLECPVLEKNNIGAGCTFASSIASQ
                     LVKKKTPLEAVKNSKELVYQAILQSDRYGVKQSYAK"
     gene            2486..2956
                     /locus_tag="VC394_001322"
     CDS             2486..2956
                     /locus_tag="VC394_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEP5994789.1"
                     /translation="MQNSKIRQMSLTGILTALVVVLGRFVMLPTPTGFLTLLDAGIYA
                     VSFSFGSAQGAIVGGLSGFLIDLVAGYPQWMFHSLIAHSVQGYFAGWRGRKRWLGVVI
                     GSFIMIFWYFLGSLMLGYGLSGSLAGIWGNVMQNTLGLFVGFIIFKAILRQKKR"
     gene            2970..3530
                     /locus_tag="VC394_001323"
     CDS             2970..3530
                     /locus_tag="VC394_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEP5994790.1"
                     /translation="MLNNLEKQTREIVIDVVERSAIQPGNLFVLGLSSSEILGSRIGK
                     QSSLEVGQIVVEVVLDELNKRGVHLAVQGCEHVNRALVVERHVAESKQLEIVNVVPNL
                     HAGGSAQMAAFQFMSDPVEVEEVIAHAGLDIGDTAIGMHIKRVQIPLIPCQRELGGAH
                     VTALASRPKLIGGARADYNMDIIRKS"
     gene            complement(3595..4440)
                     /locus_tag="VC394_001324"
     CDS             complement(3595..4440)
                     /locus_tag="VC394_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEP5994791.1"
                     /translation="MTFILHYLEQSHIENIGLTIFKKLVSLIILLLFFAILKRVTNYL
                     FEKTINKSFAYSRQSEARKKTLSKLTHNILNYLLYFLLIYWILSLFGIPVSSLLAGAG
                     IAGVAIGLGAQGFLSDVVNGFFILFENQFEVGDNVTISDIEGSVFGVGIRTTQIRGFD
                     GTLHFIPNRSITVVSNKSRGNMRALIEIPLYSTVNLSQVTRIIDEVNQKELPNHPQIV
                     GKPNILGPQNNSNGQFTFRIAIFTENGEQFKIYHTFYRLYQEALLKEGIQLPTAISYS
                     ITSKS"
     gene            4761..6044
                     /locus_tag="VC394_001325"
     CDS             4761..6044
                     /locus_tag="VC394_001325"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEP5994792.1"
                     /translation="MSTSFENKATNRGVITFTISQDKIKPALDKAFNKIKKDLNAPGF
                     RKGHMPRPVFNQKFGEEVLYEDALNIVLPEAYEAAVTELGLDVVAQPKIDVVSMEKGK
                     EWTLSAEVVTKPEVKLGDYKNLVVEVDASKEVSDEDVDAKIERERQNLAELIIKDGEA
                     AQGDTVVIDFVGSVDGVEFDGGKGDNFSLELGSGQFIPGFEDQLVGAKAGDEVEVNVT
                     FPESYQAEDLAGKAAKFMTTIHEVKTKEVPELDDELAKDIDEDVDTLEDLKVKYRKEL
                     EAAQETAYDDAVEGAAIELAVANAEIVDLPEEMIHEEVNRSVNEFMGNMQRQGISPEM
                     YFQLTGTTQEDLHNQYSAEADKRVKTNLVIEAIAKAEGFEATDSEIEQEINDLATEYN
                     MPADQVRSLLSADMLKHDIAMKKAVEVITSTASVK"
     gene            6256..6831
                     /locus_tag="VC394_001326"
     CDS             6256..6831
                     /locus_tag="VC394_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEP5994793.1"
                     /translation="MKLDVFAGQEKSELSMIEVARAILEERGRDNEMYFSDLVNEIQN
                     YLGKSDAGIRHALPFFYTDLNTDGSFIPLGENKWGLRSWYAIDEIDEEIITLEEDEDG
                     AQKRKKKRVNAFMDGDEDAIDYRDDDPEDEDFTEESAEVEYDEEDPDDEKSEVESYDS
                     ELNEIIPEDDFEEVDINEEDEEDEEDEEPVL"
     gene            7088..8692
                     /locus_tag="VC394_001327"
     CDS             7088..8692
                     /locus_tag="VC394_001327"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEP5994794.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NIDPGTMSPYQHGEVYVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITDALKEKIKRAASTTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGSENVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPVE
                     QGIKNKLAQFCDVNSEAVIESRNVEHLYQIPLNLQAQSMDQIVCDHLKLNAPQADMTE
                     WSAMVDKVMNLRKTTKIALVGKYVELPDAYLSVVEALKHSGYANDTAIDLKWVNANDV
                     TVDNAADLLGDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGICLGMQLTCVEFA
                     RHVLNMEGANSFELEPSTKYPIIDIMRDQIDIEDMGGTLRLGLYPCKLKPGSKAAMAY
                     NNQEVVQRRHRHRYEFNNKFRPEFEAAGFVFSGVSPDNRLVEIVELKEKKFFVAAQYH
                     PELQSRPNRPEELYTAFVTAAIKNSN"
     gene            8860..9786
                     /locus_tag="VC394_001328"
     CDS             8860..9786
                     /locus_tag="VC394_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEP5994795.1"
                     /translation="MKTIRIAKYLGILFLLITLISVGASFYFFHVAQIREEKSFINNK
                     KRSTNNPLYPAEQSFDALPYEKRQLTNRGLKQVGWYLPAAQKTKKTAIVVHGFTNDKE
                     DMKPYAMLFHDLGYNVLMPDNEAHGESEGNLIGYGWNDRLNVMAWTDQLIKENPESQI
                     TLFGLSMGAATVMMASGERLPAQVTSLIEDCGYTSVWDELKFQAKAMYNLPAFPLLYE
                     VSALSKIRAGFSYGEASSVKQLAKNKRPTLFIHGDKDDFVPTKMVYDNYKATKGPKEI
                     LIVKGAKHAKSFETNPEQYQKKIAAFLKKVEK"
     gene            9840..9925
                     /locus_tag="VC394_001329"
     tRNA            9840..9925
                     /locus_tag="VC394_001329"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9874..9876,aa:Leu,seq:aag)
     gene            10132..11013
                     /locus_tag="VC394_001330"
     CDS             10132..11013
                     /locus_tag="VC394_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEP5994796.1"
                     /translation="MAIVSAEKFVQAARENGYAVGGFNTNNLEWTQAILRAAEAKQAP
                     VLIQTSMGAAKYMGGYKVCQSLITNLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLAKTAEVVKIAHAKGVSVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPENWEGLALDHLEKLTAAVPGFPIVLHGGSGIPDDQI
                     KEAIRLGVAKVNVNTESQIAFSNATREFARNYEANEAEYDGKKLFDPRKFLAPGMKAV
                     QGAVEERIDVFGSANKA"
     gene            11395..11583
                     /locus_tag="VC394_001331"
     CDS             11395..11583
                     /locus_tag="VC394_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEP5994797.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRTVKPNLQKVTILVDGKP
                     KKVWASARALKSGKVERI"
     gene            11735..12100
                     /locus_tag="VC394_001332"
     CDS             11735..12100
                     /locus_tag="VC394_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEP5994798.1"
                     /translation="MTVKINTKDGLIELSDDVIATVVGGSATEIFGVVGMASKSAIKD
                     NFQSLLRKENYAKGVVVKSTDLGISVDVYTVMSYGVKISEVSKNIQERVKFNLESQLG
                     LTADMVNVYVQNIKVVGEN"
     gene            12100..13764
                     /locus_tag="VC394_001333"
     CDS             12100..13764
                     /locus_tag="VC394_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEP5994799.1"
                     /translation="MSNITTSLFQEMVQAAATRLGKQAEYVNSLNVFPVPDGDTGTNM
                     SMTMDNGAKEVADKPASTVGEVGQMLSKGLLMGARGNSGVITSQLFRGFGQSIKGKDE
                     LTGKDLAQAFQVGVEVAYKAVMKPVEGTILTVSRGAATAALKKADLTDDAVEVMQAAL
                     DGAKGALAKTPDLLPVLKEVGVVDSGGQGLVFIYEGFLSALNGDYVTSADFKATPANM
                     SEMINAEHHKSVVGHVATEDITYGYCTEIMVALKQGPTYVKEFNYDEFQGYLSGLGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLIKIKVDNMRNQHEAQVQKTDVEKNKA
                     EVKEFGLIAVVAGEGLSEIFKAQGVDYVISGGQTMNPSTEDIVKAIEAVNAKQVIILP
                     NNKNIFMAAQSAAEVVDIPAAVVATRTVPQGFTSLLAFDPSKSLEDNVADMSTSLSDV
                     VSGSVTLAVRDTTIDGLEIHENDFLGMVDGKIIVSNPDMEATLKAAFEKMIDEDSEIV
                     TIFVGEEGDQDLAEELAGYLGETYEDVEVEIHQGDQPVYPYLMSVE"
     gene            14001..14891
                     /locus_tag="VC394_001334"
     CDS             14001..14891
                     /locus_tag="VC394_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEP5994800.1"
                     /translation="MLGPFIFIAFGVIVILAIVASTLYVVRQQSVAIVERFGRYQKTA
                     TSGIHVRLPFGIDKIAARVQLRLLQSEIIVETKTKDNVFVTLNVATQYRVNEQNVTDA
                     YYKLMKPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMSTYGYIIV
                     KTLITKVEPDAEVKQSMNEINAAQRKRVAAQELANADKIKIVTAAEAEAEKDRLHGVG
                     IAQQRKAIVDGLAESIQELKEANISLNEEQIMSILLTNQYLDTLNTFATKGNQTLFLP
                     NTPSGVEDIRTQVLSALKTK"
BASE COUNT         4898 a         2401 c         3289 g         4462 t
ORIGIN      
        1 tttatccgcc atagctcagt tggtagtagc gcatgactgt taatcatgat gtcgtaggtt
       61 cgagtcctac tggcggagta gagagaggta ggaagcgtcc tatctttttt tgcattttag
      121 agaagagtgt ggtaagatag aggcaagata tgcgttggca cccttagctc aactggatag
      181 agtacctgac tacgaatcag gcggttagag gttcgactcc tctagggtgc ataatgaaaa
      241 aagcaacagg acattagtgt cctgttgttt ttttgtggtt ctctttatac tagggatagt
      301 taaaaagaaa aggagactca aaatgtcgat agagacaaga gcagcgtttg aaaaagttaa
      361 gcccattatt ttgaaattaa agcgacacta ttatattcaa ttgtgggata gagatgactg
      421 gttacaagaa ggacatatta tcttattaca gttactagaa aggtacccag agttaattga
      481 ggaagaagag cgtttatatc gctattttaa aacaaaattt tcatcttatt tgaaagattt
      541 attatgccgt caagaaagtc aaaagcgtca gttccataag ttagcatatg aagagatagg
      601 ggaggttgca catgccattc catcgagagg gttatggcta gacgactatg tggcttatca
      661 agaggtaata gctagcttag agaaccaatt aaattcgcaa gagcgtatgc agtttcaagc
      721 acttatcagg ggcgaacgtt tcaagggaag acgtgctcta cttaggaaga tcagtcccta
      781 ttttaaggaa tttgcacagc agttgtaagc aagacctata aggaattagg ggtaaatcta
      841 cgaaaaaagg agagagtttg gaggaaatag ctttcttata gcttttaaat cgctagtggc
      901 ttagagtaag cttcaatacg tctttcattt gtttgggtac agtttgatta aaaatagttt
      961 ctcttacgtt taagaaatgt taaaatagta acatggtaag atacaaagca acgatttcct
     1021 atgatgggac tcttttttcg ggttttcaaa gacaaagaca tttacgtacg gttcaagaag
     1081 agattgaaaa aacactctat aaattaaata acggtacaaa aattataatt catggtgcag
     1141 gacgtacgga tgcaggagta catgcttatg gacaggtgat ccattttgat ttgcctcaag
     1201 aacaagaagt tgaaaaatta cgatttgctc ttgatacaca gactcctgaa gatattgatg
     1261 ttgttaatat agaaaaagtg gctgatgatt ttcattgtcg ttatcagaag catttaaaaa
     1321 cttatgaatt tttagttgat aatggtcgac ctaaaaatcc gatgatgcga cactatacca
     1381 ctcattaccc ttatactctt aatattaaac ttatgcaaga agctattaac ggtctggtag
     1441 gtacacacga ttttactggt tttacagcag caggaacttc ggtacaaaat aaggtgcgaa
     1501 cgattaccaa agcaacggta tctcgggatg aaaaaactga ttttttggtg tttacttttt
     1561 caggaaatgg ttttctctat aagcaggtga ggaatatggt gggaactttg ctgaaaattg
     1621 gtaatggtca aatgccagtt gaacaagtta aagtgatttt gagctccaaa aatcgtcagc
     1681 tagctggacc aacgatatct ggaaatgggc tttacttgaa ggagatttgt tatgaaaact
     1741 gattatatcg tgactatatc tgggaacgat attttaagtg gaggtggtct ctatgctgat
     1801 ttagcgactt acataaggta tgacctacag gcctttgtag cggtaacttg tttaactact
     1861 agatctgaag agggctttag tctatttccg gtagctaaag aaattttccg tgatcagttg
     1921 aatagcttta cgaacgctcc aatttctgct attaaaattg gtttgttacc taatgcggaa
     1981 atgtgtgaaa tagttttgga ttttataaag gggcatctgg gaatcccagt ggttttagat
     2041 cctgttctag catgtaaaga aatagatgat gttaaaatag ttcctctaag acaggagatt
     2101 ttacaattat tgccttatgt aactgttgtt actccaaatt tggtagaggc tcagctatta
     2161 tctcaaaaag agattgtttc tttaaaagat atgcaagaag ctgctaaata tttttatcaa
     2221 cttggggcaa aacaggtggt tatcaagggt ggtaatcgat ttagtcagaa aaaggctatt
     2281 gatctttttt atgacggtaa agagattgtt accttggaat gtcctgtact tgagaaaaat
     2341 aatattggcg caggctgtac ttttgcatct agtatcgcta gtcagttagt aaagaaaaaa
     2401 acgccactag aagctgtgaa aaactcaaaa gagttagtct accaagctat tttacaatct
     2461 gatagatacg gagtaaaaca aagttatgca aaatagtaag atacgacaaa tgagtttaac
     2521 agggatattg acagcattgg ttgttgtttt aggaagattt gtcatgctac ctactccaac
     2581 aggtttttta acgctgttgg atgcaggaat ttatgctgtc agcttttcct ttggatcagc
     2641 tcaaggagct attgtgggag ggttatcagg ttttttaatc gatttagtgg caggataccc
     2701 tcagtggatg tttcatagtt tgattgctca cagtgtccaa gggtattttg cgggatggag
     2761 agggcgcaag cgttggcttg gtgttgtgat aggttctttt atcatgattt tttggtactt
     2821 tttaggctct cttatgcttg gttatggcct ttcgggatct ttagccggta tttggggaaa
     2881 cgtcatgcaa aatacattag ggctttttgt tggttttatt atctttaagg ccatattaag
     2941 gcaaaaaaag agataaggag agtgagaagt tgttgaataa tttagagaaa caaacaaggg
     3001 aaatcgtcat tgatgttgta gaacgctctg ctattcaacc aggtaatctt tttgttctag
     3061 gtctttcatc tagcgaaatt ttgggtagta gaattggaaa acaatctagt ctagaagtag
     3121 gtcaaatagt tgttgaagtt gtgttagatg aattaaataa gcgtggtgtt catttggctg
     3181 tacaaggttg tgaacacgtc aatcgtgcct tggtagtaga gaggcatgtg gcagaatcta
     3241 agcagttaga gattgtcaat gtggttccga atcttcatgc tggtggcagt gctcagatgg
     3301 ctgcttttca atttatgtca gaccctgtcg aagtagaaga agttattgca cacgctggac
     3361 ttgatatagg tgatactgct ataggaatgc atattaagcg tgttcagatc cctctgattc
     3421 cttgtcaacg agagctagga ggtgctcatg tgacagcttt ggctagtcga ccaaaattaa
     3481 ttggtggagc tagggctgat tacaatatgg atattatccg taaatcctaa accctttttt
     3541 ctggaataaa acaattggtg ttttttctag aaaaaaggat tttttaagat tatattaaga
     3601 ttttgaggtg atagaatagc tgattgcagt cggtaattga atgccttctt tgagtaaagc
     3661 ttcttgataa agtctataaa aagtatggta aatcttaaat tgttctccat tttctgtgaa
     3721 aatagcaatt ctaaaagtga actgtccatt agagttattt tgaggcccta aaatatttgg
     3781 tttgccaacg atttgaggat ggtttggcaa ttccttttga ttcacttcat caatgattcg
     3841 agtgacttgg ctcaaattaa cagtggaata aagaggaatt tcaattaagg cacgcatatt
     3901 accacgagat ttgttgctga caacggtgat gctacggttt gggatgaaat gtagtgttcc
     3961 atcgaatcca cgaatttgag tagttctaat tccaacacca aagacactgc cttctatgtc
     4021 agagatagta acgttatctc ctacttcaaa ttgattctca aataagataa aaaagccatt
     4081 gacaacgtca gataaaaatc cctgtgcacc taacccgatt gctactccag caattccagc
     4141 tcctgctaga agactagaga caggtatgcc aaataagcta agtatccaat aaatgagaag
     4201 aaaatacaat aagtaattta aaatattgtg agtgagctta gagagtgttt ttttgcgagc
     4261 ttcactttgt cttgaataag caaacgattt gttaatggtt ttttcaaaaa gatagttagt
     4321 aacacgtttt aaaatagcaa aaaagagtaa tagaataatg agagacacta gctttttaaa
     4381 aatcgttaag ccaatatttt cgatatgaga ctgctctagg taatgtaata taaatgtcat
     4441 gagaaatcct ttctttgata gtttatcaaa aaaaaaccaa tcttgaaaag agataaatta
     4501 atattttaac caaagttatg ataattgttt tatcataaag aatgccatag tcatccgttg
     4561 tgataatgct tgaaaatagt cagttagtaa gcgaaaaagt ctaacattaa cctttataaa
     4621 ttgaaaataa ctgttagtag actaatgaca ggtagatact cttaatagcg cgtttgattt
     4681 cacgcataaa aagctttaaa aacgctttaa attatgataa aatgaactgt atcgaaatta
     4741 ttttaaggag aaatgactta atgtctacat catttgaaaa caaagctaca aatcgtggcg
     4801 tgattacatt tacaattagt caagataaaa taaaaccagc tcttgataaa gcttttaata
     4861 aaatcaaaaa agacttaaat gcaccaggtt tccgtaaagg acacatgcct cgtccagtct
     4921 tcaaccaaaa atttggtgaa gaagttcttt atgaagatgc tttgaatatt gtattgccag
     4981 aagcttatga agcagctgtg acggaacttg gtcttgatgt ggttgcacaa ccaaaaatcg
     5041 atgttgtgtc aatggaaaaa gggaaagagt ggacactttc tgctgaagtt gtgacaaaac
     5101 ctgaagtgaa acttggtgat tacaaaaacc ttgttgtaga agttgatgct tcaaaagaag
     5161 tttcagatga agacgtggat gctaaaattg agcgcgaacg tcaaaacctt gcggaactca
     5221 ttattaaaga cggtgaagca gctcaaggtg acactgttgt gattgacttt gttggttcag
     5281 ttgatggtgt tgagtttgat ggcggtaaag gagataactt ctctcttgaa cttggttcag
     5341 gacaatttat cccaggtttt gaagatcaac tagttggtgc taaagctggc gatgaagtag
     5401 aagttaatgt cacattccca gaatcttacc aagcagaaga tcttgcaggt aaagccgcta
     5461 aatttatgac aactattcac gaagtcaaaa caaaagaagt accagagctt gatgatgagc
     5521 ttgcaaaaga tattgatgaa gatgttgaca cacttgaaga cttaaaagta aaatatcgta
     5581 aagaacttga agcagctcaa gaaactgctt atgatgatgc tgttgaagga gctgcgattg
     5641 aattagcagt tgcaaatgct gaaattgttg atttacccga agaaatgatt catgaagaag
     5701 tcaaccgttc agtgaatgaa tttatgggca acatgcaacg tcaaggaatc tcacctgaaa
     5761 tgtacttcca attgactggt acaactcaag aagatttaca taaccaatat tcagctgaag
     5821 ctgacaagcg tgttaaaaca aaccttgtta ttgaagcaat tgctaaagca gaaggttttg
     5881 aagcaacaga tagtgaaatt gaacaagaaa ttaatgacct tgcaacagaa tataacatgc
     5941 cagctgacca agttcgttct cttctttcag cagatatgtt gaaacatgat attgcaatga
     6001 aaaaagcagt tgaagtgatt acaagcacag caagcgttaa gtaatcttat taaggaacaa
     6061 aacgtacaag ccggattaga acgtctttct agtctggttt ttctttttct aaagataagg
     6121 aattagttaa acgttatttt aatgaattag ctttaaagat ttgtcaagaa gaagtgatat
     6181 gagttgataa aagttttgac gaattacttt ttttagcgta aaatggtaga gaatgataaa
     6241 ataaggagaa ctgccttgaa attagacgta tttgcaggac aagaaaaaag cgagctttcc
     6301 atgattgaag tggcgcgtgc cattttggaa gaacgcggtc gcgacaatga gatgtacttt
     6361 agcgatttag tcaatgagat tcaaaattac ttaggaaaat ctgatgcagg tattcgtcat
     6421 gcattaccat ttttctatac tgacttaaat acggatggct catttattcc tctaggtgaa
     6481 aacaaatggg gacttcgttc gtggtatgct attgatgaaa ttgatgaaga aattattact
     6541 ctagaagaag atgaagatgg cgcacaaaaa cgtaagaaaa aacgcgttaa tgctttcatg
     6601 gatggagatg aagacgctat tgattataga gatgatgatc cagaagatga agatttcaca
     6661 gaagaatcag ctgaggttga atatgatgaa gaagatccag atgatgaaaa atcagaagtg
     6721 gagtcatatg attcagaatt gaatgaaatt atccctgaag atgattttga agaagttgat
     6781 atcaatgaag aagacgaaga agatgaagaa gatgaagaac cagttctcta atgtctttag
     6841 ttcagaaata aaaatgtgat tatcatttga caaattgtct attatagttt atattattat
     6901 tcgggcacct ctttatgagg tcaggcaaaa gctctctagt taataggggg ctatttttgt
     6961 tttctagaat aatgccagtt tgcctctata tttagaaaac taaggtgata cctaaccaat
     7021 gtggaaccaa ctaataggga attaacaaga aagcaatgct tttattcagc ttatataaag
     7081 gagtactatg acgaaataca tttttgtaac tggtggtgtt gtatcatcaa ttggcaaagg
     7141 aattgttgcg gcaagtcttg gccgattgtt aaaaaatcgt ggacttaagg tgacgattca
     7201 aaaatttgat ccttatatta atattgatcc agggacgatg agcccctatc agcatggaga
     7261 agtatatgtg acagatgacg gtgctgaaac cgacttggat ttgggtcact atgaacgttt
     7321 tattgatatc aatcttaata aatattccaa tgttacaaca ggtaaaattt atagtgaagt
     7381 ccttcgtaaa gaacgtaaag gtgagtacct tggagcgact gttcaggtga ttcctcatat
     7441 cacagatgcc cttaaagaaa aaatcaaacg cgcagcttca acaacggact ctgatgtaat
     7501 tattactgag gtcgggggga ctgttggcga tattgaaagt ttaccgttct tagaagctct
     7561 tcgtcagatg aaagctgatg ttggttctga aaatgtgatg tatattcaca ccaccttgtt
     7621 gccatattta aaggcagcgg gtgaaatgaa aactaagcca acgcaacatt ccgttaaaga
     7681 attgcgtggt ttagggattc aaccgaatat gttggttatt cgtacggaag agccagttga
     7741 gcaggggatt aaaaataaat tggctcagtt ctgtgatgtg aattcagaag ctgttatcga
     7801 gtcacgtaat gtagaacact tgtatcaaat tccattgaac ttgcaagccc aatcaatgga
     7861 tcagattgtt tgtgatcatt tgaaattgaa tgcgccacaa gctgatatga ctgagtggtc
     7921 agctatggtt gacaaggtga tgaatttaag aaaaacaaca aaaattgcct tggttggtaa
     7981 atatgtggaa ttgccagatg cctacttatc tgttgtggaa gccttgaaac attctggtta
     8041 tgccaatgat accgcaattg acctaaaatg ggtcaatgct aacgatgtga cggtggataa
     8101 tgcagctgac ttgttgggag atgcggatgg gattattgta ccaggtggtt ttggtcaacg
     8161 tggtacagaa ggcaagatcc aagcgattcg ctatgcccgc gagaatgatg tgccaatgct
     8221 tggtatctgt cttggcatgc aactgacttg tgttgaattt gctcgccatg tcctcaatat
     8281 ggaaggtgca aactcttttg aattagagcc aagtactaaa taccccatca ttgatatcat
     8341 gcgtgatcaa attgatattg aagatatggg aggtacgctt cgtctgggat tgtacccatg
     8401 taaattgaaa ccaggttcaa aagcagcaat ggcttacaat aatcaagagg tggtgcaacg
     8461 tcgtcatcgt caccgttatg agttcaacaa caaattccgt ccagaatttg aagcagctgg
     8521 ttttgttttc tcaggggtat cacctgacaa ccgcttagtt gaaattgtag aattgaaaga
     8581 gaaaaaattc tttgtagcag ctcaatacca cccagaattg caaagccgtc caaatcgtcc
     8641 tgaggagcta tacaccgctt ttgttacagc tgctattaaa aatagcaatt agtggtttta
     8701 agggtaagtt tgtggcattc tacttggctg gcagatactg ataaagatta ctagctatta
     8761 gagggatctt ttctataaaa atagcattag aaaaagaaaa acgtgatatt tttaggttag
     8821 cactttcttt ttcagtcatt tttttgtaaa atacttggta tgaaaactat tcgaatagca
     8881 aagtatttag gaattctatt ccttttgata actcttatta gcgtaggtgc tagtttttat
     8941 ttctttcatg ttgcacaaat aagagaagag aaatcgttta ttaataataa gaaacggagt
     9001 acaaataatc cattataccc agctgaacag tcttttgacg ctttacctta cgaaaaacgt
     9061 caactaacaa atcgtgggtt aaaacaagtg gggtggtact taccagctgc tcaaaaaaca
     9121 aaaaagacag ctattgttgt tcatggtttt acgaatgaca aagaagatat gaagccatat
     9181 gccatgcttt ttcatgattt gggctataat gtcttaatgc cagacaatga ggcccatggg
     9241 gaaagtgaag ggaacttgat tggttatggc tggaatgacc gccttaatgt catggcttgg
     9301 acagaccaac tgattaagga aaaccctgaa agccaaatca cactctttgg cttatctatg
     9361 ggtgctgcaa cagtaatgat ggcaagtggt gagcgattgc ctgcgcaagt cacctccctc
     9421 atcgaagatt gcggttatac cagtgtttgg gacgaattga agtttcaggc caaggctatg
     9481 tacaacttgc ctgcctttcc tttactctat gaagtctctg ccttatctaa gattcgagca
     9541 ggttttagtt acggagaagc gagctcagtg aaacagctgg ctaaaaataa acgtccaact
     9601 ttatttatcc acggtgataa ggatgatttt gttcctacaa aaatggttta tgacaattat
     9661 aaggccacga aaggtcctaa ggaaatcttg attgttaaag gggcaaaaca cgccaaatcc
     9721 tttgaaacaa acccagaaca ataccagaaa aaaattgccg cttttttgaa aaaagttgag
     9781 aaataagtat tgacagttaa actcacagtt gctataataa tttatgttac tgatgagtag
     9841 cggggatggc ggaattggca gacgcgcagg actaaggatc ctgtgaccgc tttaggtcgt
     9901 gtgggttcaa gtcccactct ccgcataatg taagaggtga atcattgatt cgtctctttt
     9961 ttttggttaa atgaaggata agaaggcata gatttactgg agaaaacttt tgataatctg
    10021 cttttggaaa acgctttaag cttgtgaaag actagaaatt tatggaaaat tgtgctagaa
    10081 tgaacaatgt aaacggggag ttatcccgaa aaataagagg aggccttaca aatggcaatc
    10141 gtttcagcag aaaaatttgt ccaagcagct cgtgaaaacg gatatgctgt tggtggattt
    10201 aacacaaaca acttagagtg gactcaagca atcttgcgtg cagcagaagc taaacaagct
    10261 ccagttctta tccaaacatc aatgggtgca gcaaaataca tgggtggtta taaagtatgt
    10321 caatctctta ttactaacct tgttgagtca atgggtatca ctgttcctgt tgctatccac
    10381 cttgatcatg gtcactacga agatgctctt gagtgtatcg aagtaggtta cacttcaatc
    10441 atgtttgacg gttcacacct tccagttgaa gaaaaccttg ctaaaactgc agaagttgtt
    10501 aaaattgcac atgctaaagg cgtttcagtt gaagctgaag ttggtacaat cggtggtgaa
    10561 gaagacggta tcatcggtaa aggtgagctc gctccaatcg aagatgctaa agcaatggtt
    10621 gaaactggaa ttgacttctt ggcagctggt atcggtaaca tccacggtcc atacccagaa
    10681 aactgggaag gtcttgctct tgaccactta gaaaaattaa cagcagctgt accaggtttc
    10741 ccaatcgtat tgcacggtgg ttctggtatt cctgacgatc aaatcaaaga agcaatccgt
    10801 cttggtgttg ctaaagttaa cgttaacact gaaagtcaaa tcgctttctc taacgcaact
    10861 cgtgaatttg ctcgtaacta cgaagctaac gaagcagaat atgatggtaa aaaattattt
    10921 gacccacgta aattcttggc tccaggtatg aaagctgttc aaggtgctgt tgaagaacgt
    10981 atcgatgttt tcggatcagc taacaaagca taatttagag tattaaatac cctaaagccc
    11041 tcttgagggc ttttttagat acaattgaca ccctaaaatg gataccagtt tctgtgaaac
    11101 aatctttgat tgtgaacaaa caaagcataa tttagagtat taaaatctgg accctgctat
    11161 ctatggtaag tctttttgtt attgttaaag aggttaagcg gaatgttaat atgttttagc
    11221 taaaaaatgt agtgaaaaat gagtacgtag actattgtaa tagtaattcc gtaaaattgt
    11281 tgaaaaagaa aatgggaaat accttgtcaa attaagcacc ctatgataga atagataggt
    11341 atgtggtgct agcacatccg taaacatatt ctacgtagga ggaaacagat caaaatggct
    11401 aaagtatgtt attttacagg acgtaaaacc gtttctggaa acaaccgttc acacgcgatg
    11461 aaccaaacga aacgtacagt taaaccaaac cttcaaaaag ttacgatcct tgttgatggt
    11521 aaacctaaaa aagtttgggc ttctgctcgt gcgcttaaat ctggtaaagt agaacgcatc
    11581 taatagaaat aagccttagg gcttttttta ttagcttatt tctatccata agctagtcag
    11641 ggtaataaac gctatcgtta actattctat ctcttttaca ttacttctaa aatatgataa
    11701 aataggatga taaaaacttt ttgaggtagt aactatgact gtaaaaatta atacaaaaga
    11761 tggtctaatc gagctatccg acgatgtgat tgcaactgtc gtgggaggat cagcaacgga
    11821 aatttttggt gttgttggaa tggccagcaa aagtgcaata aaagacaatt ttcaatcact
    11881 acttcgcaaa gaaaactacg ctaagggtgt tgtagtaaaa tcaacagatc tgggtatttc
    11941 tgttgatgtc tacactgtga tgagttatgg tgtgaaaatc agtgaagtct ctaaaaatat
    12001 ccaagaacgc gtgaaattta accttgaaag tcagcttggt ctcactgctg atatggtcaa
    12061 tgtttacgtg caaaatatta aggttgtagg agaaaattag tgtcaaatat tacaacaagc
    12121 ttattccaag aaatggtcca agcggcagcg actcgccttg gcaaacaagc agaatatgtt
    12181 aattccttaa atgtttttcc tgttccagat ggtgatacag gaacaaacat gagtatgact
    12241 atggacaatg gtgccaagga agttgctgat aaacctgcgt caactgtggg agaagttgga
    12301 caaatgttat caaaaggtct tttgatggga gcgcgtggaa attctggagt tattacttca
    12361 caactttttc gtggttttgg tcaaagtatt aagggaaaag atgagctgac aggtaaagat
    12421 ttggcacaag cctttcaagt gggtgtagaa gttgcctaca aagcagtgat gaaacctgtt
    12481 gaagggacaa tcttgacagt atctcgtgga gcagcaaccg ctgccttaaa aaaagcggat
    12541 cttacagacg acgccgtgga agtcatgcaa gctgctttag acggtgccaa aggtgccctt
    12601 gctaagacac ctgatttatt accagtcctt aaagaagttg gtgtggttga ttcaggcggt
    12661 caaggtcttg tctttatcta tgaaggtttc ctatctgctt tgaatggcga ctatgtgact
    12721 tcagcagact ttaaagcgac tcctgctaac atgtctgaaa tgattaatgc tgagcatcac
    12781 aaatccgttg ttggtcatgt ggctactgaa gacattacct acggttattg tactgaaatc
    12841 atggttgccc ttaaacaagg cccaacctat gtgaaagaat tcaactatga tgagtttcaa
    12901 ggctacctta gtggccttgg tgactcgctg ttagtggtca atgatgatga gattgttaaa
    12961 gttcacgtcc atactgaaga ccccggactt gtgatgcaag aaggtcttaa atatggttcg
    13021 ctcatcaaga ttaaagtgga caatatgcgt aaccagcatg aagcacaggt tcaaaagact
    13081 gatgtcgaaa aaaacaaggc tgaagtaaaa gaatttggct tgattgcagt agtagctgga
    13141 gaaggtttat ctgaaatttt caaggcgcaa ggtgttgatt acgtgatttc tggtggtcaa
    13201 accatgaatc catctacaga agatattgtt aaagctattg aagcagttaa tgccaaacaa
    13261 gtcattatct tgccaaacaa taaaaatatc tttatggctg cccaatctgc tgcagaagtg
    13321 gtggatatcc cggctgctgt tgttgcaaca cgtactgttc cgcaaggatt taccagcttg
    13381 ttggcctttg atccttcaaa atctttagag gacaatgtag cagatatgtc aactagtctt
    13441 tctgacgtgg tcagtggaag cgttactttg gcagtccgtg acaccaccat tgatggttta
    13501 gaaatccatg aaaatgattt cttgggaatg gttgatggta aaatcatcgt ttcaaaccca
    13561 gacatggaag caactctaaa agctgccttt gaaaagatga ttgacgaaga tagcgagatt
    13621 gtgactatct ttgttggtga agaaggtgat caagacttag cagaagaact tgctgggtat
    13681 ttaggagaga cttacgaaga tgtggaagtt gagatccatc aaggagatca gcctgtttac
    13741 ccatatctca tgagtgtaga ataatagaat acaataaaaa aagaggagtt aggcaaaggt
    13801 ctgactcttt ttgtctatat tgatgtcgta atgagtggtt ttagtgatgg taagccccta
    13861 aaaaatgagg gtaatccatc acaaagctaa tggttacgat ttagtttgac aaaacctaga
    13921 aaaacattat aattgtatta taacaataag tcaagaactg aaaacggttc agttctaaga
    13981 tatgacaagg aggagatttt atgttaggac catttatctt tattgctttt ggggttattg
    14041 taatcctagc tattgttgcc agtacccttt atgttgttcg tcaacaatct gttgccatcg
    14101 tggaacgttt tggtaggtac caaaaaacag ccacgagtgg tattcatgtc cgccttccgt
    14161 ttgggattga caaaattgct gcgcgtgtgc agctgcgtct tttacagtca gaaatcatcg
    14221 ttgaaaccaa aaccaaggac aatgtgtttg taactttgaa tgttgccact cagtatcgtg
    14281 ttaacgaaca aaatgtaaca gatgcttact acaaattaat gaaacctgaa tctcaaatca
    14341 agtcttatat tgaagatgcc ttgcggtcat ccgtgccgaa gttgacctta gacgaattgt
    14401 ttgagaaaaa agatgaaatt gccttggaag tacaacatca agtggcagaa gaaatgtcaa
    14461 cctatggata tattattgtc aaaaccttga ttaccaaggt ggagcctgat gctgaagtca
    14521 aacagtcaat gaatgaaatc aatgcggctc aacgcaaacg tgtagctgct caagagttgg
    14581 caaacgcaga taaaattaaa attgtgacag cagcagaagc agaggctgaa aaagatcgat
    14641 tgcacggggt agggattgcc caacagcgta aggccattgt ggatggtttg gcggagtcta
    14701 ttcaagagct taaagaagcg aatatttcct taaacgaaga acaaatcatg tccattttgt
    14761 tgaccaacca atacctggat accctcaata cctttgcgac aaaaggaaac caaacccttt
    14821 ttctaccaaa tacaccaagt ggggtagagg atatccgtac acaagtttta tctgccctta
    14881 aaacaaaata acgtgtaaaa gcttggctaa aaagttgctc aatactggat ttagcagaag
    14941 gctatcatac cttgtgaagt ggcaaacgac gaaatgaata cattactttt attggagaaa
    15001 tgaaagtaaa aaaagccttt ttcatgatta acaacatagt attgattaaa
//
LOCUS       DATXQK010000040        13411 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.040, whole genome shotgun sequence.
ACCESSION   DATXQK010000040 DATXQK010000000
VERSION     DATXQK010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13411
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.040"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..641
                     /locus_tag="VC394_001335"
     CDS             <1..641
                     /locus_tag="VC394_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin binding protein"
                     /protein_id="HEP5994801.1"
                     /translation="DGQEVPEKPSVDLPIEVPRYEFNNKDQSPLAGESGETEYITEVY
                     GNQQNPVDIDKKLPNETGFSGNMVETEDTKEPEVLMGGQSESVEFTKDTQTGMSGQTT
                     PQVETEDTKEPGVLMGGQSESVEFTKDTQTGMSGFSETVTIVEDTRPKLVFHFDNNEP
                     KVEENREKPTKNITPILPATGDIGNVLAFLGILILSVLSIFSLLKNKQNNKV"
     gene            929..1777
                     /locus_tag="VC394_001336"
     CDS             929..1777
                     /locus_tag="VC394_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEP5994802.1"
                     /translation="MTERLKNLGILLLFLLGTAIFLYPTLSSQWNAYRDRQLLSTYHK
                     QVTQKKPSEMEEVWQKAKAYNARLGIQPVPDAFSFRDGIHDKNYESLLQIENNDIMGY
                     VEVPSIKVTLPIYHYTTDEVLTKGAGHLVGSALPVGGDGTHTVISAHRGLPSAEMFTN
                     LNLVKRGDTFYFRVLNKVLAYKVDQILTVEPDQVTSLSGVMGKDYATLVTCTPYGVNT
                     KRLLVRGHRIAYHYKKYQQAKKAMKLVDKSRMWTEVVCAAFGVVIAIILVFMYSKMTD
                     KKVIKN"
     gene            2063..4306
                     /locus_tag="VC394_001337"
     CDS             2063..4306
                     /locus_tag="VC394_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus ancillary protein 1"
                     /protein_id="HEP5994803.1"
                     /translation="MNNKKLQKKQDAPRVSNRKPKQLTVTLVGVFLMLLVLIGFEGKV
                     RAAHELVEVPVPIFHNPDPQSDYQWYGYEAYTGGYPKYDLFKTYYHDLRVNLHGSKSY
                     QVYCFNVHKHYPRSSQSFDRKWYKKLDGTAENFDSLAMEPRVRKEELTKKLLAVMYNA
                     YPNDANGIMKDLEPLNAIKVTQEAVWYYSDSAQINPDESFKTEAQSNGINDQQLGLMR
                     KALKELIDPNLGSKYSNKTPSGYRLNVFESHDKTFQNLLSAEYVPDTPPKPGEEPPAK
                     TEKTSVIIRKYAEGDYSKLLEGATLRLTGEDIPDFQEKVFQSNGTGEKIELSNGTYTL
                     TETSSPDGYKIAEPIKFRVVNKKVFIVQKDGSQVENPNKEVGSPYTIEAYNDFDEFGL
                     LSTQNYAKFYYGKNYDGSSQIVYCFNANLKSPPDSEDHGATINPDFTTGDIRYSHIAG
                     SDLIKYANTARDEDPQLFLKHVKKVIENGYHKKGQAIPYNGLTEAQFRAATQLAIYYF
                     TDSVDLTKDRLKDFHGFGDMNDQTLGVAKKIVEYALSDEDSKLTNLDFFVPNNSKYQS
                     LIGTEYHPDDLVDVIRMEDKKQEVIPVIHSLTVKKTVVGELGDKTKGFQFELELKDKT
                     GQPIVDALKTNNQDLVAKDGKYSFNLKHGDTIRIEGLPTGYSYTLKETEAKDYIVTVD
                     NKVSQEAQSASENVTADKEVTFENRKDLVPPTGFTTDGGTYLWLLLLVPFGLLVWFFG
                     RKGLKND"
     gene            4299..4820
                     /gene="lepB"
                     /locus_tag="VC394_001338"
     CDS             4299..4820
                     /gene="lepB"
                     /locus_tag="VC394_001338"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEP5994804.1"
                     /translation="MTNYLNRLNENPLFKAFIRLVLKISIIGFLGYILFQYVFGVMIV
                     NTNQMSPAVSAGDGVLYYRLTDRYHINDVVVYEVDNTLKVGRIAAQAGDEVNFTQEGG
                     LLINGHPPEKEVPYLTYPHSSGPNFPYKVPTGTYFILNDYREERLDSRYYGALPINQI
                     KGKISTLLRVRGI"
     gene            4842..5879
                     /locus_tag="VC394_001339"
     CDS             4842..5879
                     /locus_tag="VC394_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QVPTGV class sortase B protein-sorting
                     domain-containing protein"
                     /protein_id="HEP5994805.1"
                     /translation="MKKNKLLLATAILATALGTASLNQNVKSETAGVSENAKLIVKKT
                     FDSYTDNEVLMPKADYTFKVEADSSATDKTKDGLEIKPGVTEGLTTEQTIAYDNSVKP
                     SDKSKTATFDFSTVKFPEVGVYRYTVSEIDSKVSGIKYDTKTWIVDVYVVNDGNGGFK
                     ARYIVSKEKGQNDKKPVVFENSFKTTSLKVEKQVTGNTGELKKDFNFTLTINPNDNFV
                     AGQVIKLEKGGIKADVKIGEPYKFALKNGEKVTLSKLPVGVTYSIIEDEADKDGYTTN
                     AKITDGTAAPVEYKLGNQQLADESADEIVVTNNRDTQVPTGVVGTLAPFAVLSIVAIG
                     GVIYITKRKKA"
     gene            5895..6620
                     /gene="srtB"
                     /locus_tag="VC394_001340"
     CDS             5895..6620
                     /gene="srtB"
                     /locus_tag="VC394_001340"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HEP5994806.1"
                     /translation="MMMTIVQVINKAIDTLILIFCLVVLFLAGFGLWDSYHLYQQADA
                     SNFKKFKTAQQQPKFEDLLALNEDVIGWLNIPGTHIDYPLVQGKTNLEYINKAVDGSV
                     AMSGSLFLDTRNHNNFTDDYSLIYGHHMAGNAMFGEIPKFLKKDFFNKHNKAIIETKE
                     RKKLTVTIFACLKTDAFDQLVFNPNAITNQDQQRQLVDYISKRSKQFKPVKLKHHTKF
                     VAFSTCENFSTDNRVIVVGTIQE"
     gene            6637..7224
                     /locus_tag="VC394_001341"
     CDS             6637..7224
                     /locus_tag="VC394_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HEP5994807.1"
                     /translation="MRKYWKMLFSVVMILTMLAFNQTVLAKDSTVQTSISVENVLERA
                     GDSTPFSIALESIDAMKTIEEITIAGSGKASFSPLTFTTVGQYTYRVYQKPSQNKDYQ
                     ADTTVFDVLVYVTYDEDETLAAKVISRRAGDEEKSAITFKPKRLVKPIPPRQPDIPKT
                     PLPLAGEVKSLLGILSIVLLGLLVLLYVKKLKSRL"
     gene            complement(7383..8588)
                     /locus_tag="VC394_001342"
     CDS             complement(7383..8588)
                     /locus_tag="VC394_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEP5994808.1"
                     /translation="MVIFDLKYVQTLHSLSQLPISVMSQDKALIQVYGNDDYLLCYYQ
                     FLKHLAIPQAAQDVIFYEGLFEESFMIFPLCHYIIAIGPFYPYSLNKDYQEQLANNFL
                     KHSSHRSKEELLSYMALVPHFPINNVRNLLIAIDAFFDTQFETTCQQTIHQLLQHSKQ
                     MTADPDIIHRLKHISKASSQLPPVLEHLNHIMDLVKLGNPQLLKQEINRIPLSSITSS
                     SISALRAEKNLTVIYLTRLLEFSFVENTDVAKHYSLVKYYMALNEEASDLLKVLRIRC
                     AAIIHFSESLTNKSISDKRQMYNSVLHYVDSHLYSKLKVSDIAKRLYVSESHLRSVFK
                     KYSNVSLQHYILSTKIKEAQLLLKRGIPVGEVAKSLYFYDTTHFHKIFKKYTGISSKD
                     YLAKYRDNI"
     gene            8973..12467
                     /locus_tag="VC394_001343"
     CDS             8973..12467
                     /locus_tag="VC394_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin binding protein"
                     /protein_id="HEP5994809.1"
                     /translation="MTQKNSYKLSFLLSLTGFILGLLLVFIGLSGVSVGHAETRNGAN
                     KQGSFEIKKVDQNNKPLSGATFSLTPKDGKGKPVQTFTSSEEGIIDAQNLQPGTYTLK
                     EDKAPDGYDKTSRTWTVTVYENGYTKLVENPYNGEIISKAGSKDVSSSLQLEKSTMSV
                     FSKYGGTEVTSGAADFYRNHAAYFKMSFELKQKDKSETINPGDTFVLQLDKRLNPKGI
                     SQDIPKIIYDSENSPLAIGKYDAKTHQLTYTFTNYIAGLDKVQLSAELSLFLENKVVL
                     ENQTISDFKSTIGDQEIKYGGTVNVLYGNASGQDNNYVTNGLSNVGGSIESYNTQTGD
                     FTWYVYVNPNRTNIPYATMNLWGFGRLVKSNNSTSKSEDSATSSAQLEKVEIYEVKED
                     YTLPTSYGVDLTKLNLRKDLEAKLPQDSTQNSNKRLRIDFGDKLQNKAFVVKITGKTD
                     QSGKPLVVQSNLASFNNETAVTYGNPVNNVYFQNEIALSPSKGSGSGTSEFTKPSITV
                     ANLKRVAQLRFKKMSTDNVPLPEAAFELRSSNGNSQKVEAKSNTQGEVHFKDLTSGTY
                     DLYEKQAPKGYQQVTEKLATVTVDTTKPAEEMVTWGSPHSSVKVEDSKEVTIVNHKET
                     LTFSGKKIWENDRPDQRPAKIQVQLLQNGQKMSDKVQEVTKDNDWSYHFKDLPKYDDR
                     NQEYKYSVEEVKVPDGYKVSYLGNDIFNTRETEFVFEQNNFNLEFGNAEIKGQSGSKI
                     IDEDTLTSFKGKKIWKNDTAENRPQAIQVQLYADGVAVEGQTKFISGSGNEWSFEFKN
                     LKKYNGTGNDIIYSVREVTVPTGYDVTYSANDIINTKREVITQQGPKLEIEETLPLES
                     GASGGTTTVEDSRPVDTLSGLSSEQGQSGDMTIEEDSATHIKFSKRDIDGKELAGATM
                     ELRDSSGKTISTWISDGQVKDFYLMPGKYTFVETAAPDGYEIATAITFTVNEQGQVTV
                     NGKATKGDAHIVMVDAYKPTKGSGQVIDIEEKLPDEQGHSGSTTEIEDSKSSDLIIGG
                     QGEVVDTTEDTQSGMTGHSGSTTEIEDSKSSDVIIGGQGQVVETTEDTQTGMHGDSGC
                     KTEVEDTKLVQSFHFDNKESESNSEIPKKDKPKSNTSLPATGEKQHNMFFWMVTSCSL
                     ISSVFVISLKTKKRLSSC"
     gene            complement(12733..13398)
                     /locus_tag="VC394_001344"
                     /pseudo
     CDS             complement(12733..13398)
                     /locus_tag="VC394_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4601 a         2183 c         2689 g         3938 t
ORIGIN      
        1 tagatggtca agaagtccct gaaaaaccta gtgttgactt acctattgaa gttcctcgtt
       61 atgagtttaa caataaagac cagtcacctc tagcgggtga gtctggtgag acggagtata
      121 ttaccgaagt ctatggaaat caacagaacc ctgttgatat tgataaaaaa cttccgaatg
      181 aaacaggttt ttcaggaaat atggttgaga cagaagatac gaaagagcca gaagtgttga
      241 tgggaggtca aagtgagtct gttgaattta ctaaagacac tcaaacaggc atgagtggtc
      301 aaacaactcc tcaggttgag acagaagaca cgaaagagcc aggagtattg atgggaggtc
      361 aaagtgagtc tgttgaattt actaaagaca ctcaaacagg catgagcggt ttcagtgaaa
      421 cagtgaccat tgttgaagat acgcgtccga agttagtgtt ccattttgac aataatgagc
      481 ccaaagtgga agagaatcgg gaaaagccta caaaaaatat aacacctatc cttcctgcaa
      541 caggagatat tggaaatgtt ttggcctttc ttggaatcct tattttgtca gtactttcta
      601 tttttagcct tttaaaaaac aaacaaaaca ataaagtctg acgtaaaagt gttccataag
      661 aaataatgcg caaatatgct gaatagtgga taactgctac agtcaagaca agtgttaatt
      721 caaaaggtac tacttataca tctgataata ataaggtgac agataattca gagcaagtag
      781 gatggctaaa aaatgatgta ttctatgcat aatagactta caagaaatca agttattgtt
      841 aaacgtcgta aagcttaacg ctttcaaaag tcgaaatcaa tcgagactgt ctttaggcag
      901 tctcgatttt tattgataag gaactgcaat gacagaaaga ctaaaaaatc tagggatact
      961 cttattattt ttattgggaa cagccatttt tctttaccct acgctaagta gtcagtggaa
     1021 tgcctaccgt gatcgtcaat tgttatcaac ttatcacaaa caagtgactc aaaagaagcc
     1081 ctcagaaatg gaggaggtgt ggcaaaaggc taaggcgtat aacgctaggc ttgggattca
     1141 gcccgttcca gatgcttttt catttaggga tggcattcat gataaaaact acgaaagcct
     1201 acttcaaatt gaaaacaatg atattatggg atatgttgaa gtgccctcta ttaaagtgac
     1261 gttacccata taccactata cgacagacga ggtgctaact aagggagcag gtcacttagt
     1321 tggaagtgcc ttaccagttg gtggcgacgg gactcacacg gttatttctg ctcatagagg
     1381 ccttccatcg gcagaaatgt ttactaattt gaatttagtg aaaaggggag atacttttta
     1441 ctttcgtgtg ttaaataaag tattggctta taaagttgat cagatactaa ctgttgaacc
     1501 agatcaagtc acatctcttt ctggggtaat gggaaaagat tatgctactc tggttacctg
     1561 cacaccatat ggtgttaata ccaagcgtct attggtcaga ggtcatagaa ttgcatatca
     1621 ttacaaaaag tatcaacagg ctaaaaaagc aatgaaactc gtggataaat caagaatgtg
     1681 gacagaagta gtatgtgctg ctttcggggt ggttatagct attattttag tatttatgta
     1741 ttcaaagatg actgataaaa aagtgattaa aaactaactt gccctgaacc ttctttatcg
     1801 tattgcaact gtcataaaag taaaataatc tggtagttac tatccccttt gatgaagttt
     1861 aattaaatct catcaagaga aaaatattag gcgattacaa atgattttcc caaaaattat
     1921 tttttggaac gcataatatg gaaaaaaaga tgataagaac aactgtttta aacatcaaat
     1981 aaatgatata gtagctatga aatagcattg tgactgtccg gtaaagggat gggactgaag
     2041 attttattca gaaggagagg gaatgaataa caaaaaattg caaaagaagc aagatgctcc
     2101 tcgggtatca aacagaaagc caaaacaatt aactgtcact ttagtgggag tatttttaat
     2161 gctcttagta ttgataggat ttgaaggaaa agttcgagca gctcacgaat tggttgaggt
     2221 accagtacct atttttcaca atccagatcc acagtcagac tatcagtggt atggctatga
     2281 agcctataca ggggggtatc cgaagtatga tttattcaag acatactatc atgatctgag
     2341 agtgaatttg catgggagta aaagttatca agtatattgc tttaacgttc acaaacacta
     2401 tccccgttcc tcccagagtt ttgatcggaa gtggtataaa aaattagatg gaactgctga
     2461 gaattttgat tctcttgcta tggaaccaag agttcgtaaa gaggagttaa ccaaaaaact
     2521 cctagctgtt atgtataatg cctatcctaa tgatgctaat ggcattatga aagatttaga
     2581 gcctttaaat gctattaaag tcactcaaga ggctgtatgg tactattctg attcagctca
     2641 aattaatccg gatgaaagtt ttaaaacaga agctcaaagt aatggtatta atgaccagca
     2701 gttaggctta atgcgaaaag ctttaaaaga actaattgat ccaaacttag ggtcaaaata
     2761 ttcgaataaa actccatcag gttatcggtt aaatgtattt gaatctcatg ataagacttt
     2821 ccaaaatctt ttgagtgctg agtatgttcc ggatactccc ccaaaaccag gagaagagcc
     2881 tccggctaaa actgaaaaaa catcagtcat tatcagaaaa tatgcggaag gtgactactc
     2941 taaacttcta gagggagcaa ctttgcgttt aacaggggaa gatatcccag attttcaaga
     3001 aaaagtcttc caaagtaatg gaacaggaga aaagattgaa ttatcaaatg ggacttatac
     3061 cttaacagaa acatcatctc cagatggata taaaattgcg gagccgatta agtttagagt
     3121 agtgaataaa aaagtattta tcgtccaaaa agatggttct caagtggaaa atccaaacaa
     3181 agaagtaggt tctccatata ctatagaggc atacaatgat tttgatgaat ttggtttact
     3241 gtcaacacaa aattatgcga aattttatta tggaaaaaac tatgatggca gttcacaaat
     3301 tgtttattgc ttcaatgcca acttgaaatc cccacctgac tcggaagatc atggtgctac
     3361 aataaatcct gactttacga ctggtgatat taggtatagt catattgctg gttcagattt
     3421 gataaaatac gctaatacag ctagggatga agatcctcaa ttatttttaa aacacgtaaa
     3481 aaaagtaatt gaaaatgggt atcataaaaa aggtcaagct attccatata acggtctgac
     3541 tgaggcacag tttcgcgcgg ctactcaact ggcaatttat tattttacag atagtgttga
     3601 cttaactaag gatagattga aagacttcca tggatttgga gatatgaatg atcaaacttt
     3661 gggtgtagct aaaaaaattg tagaatacgc tttgagtgat gaagattcaa aactaacaaa
     3721 tcttgatttc ttcgtaccta ataatagcaa ataccaatct cttattggga cagaatacca
     3781 tccagatgat ttggttgacg tgattcgtat ggaagataaa aagcaagaag ttattccagt
     3841 aattcatagt ttgacggtga aaaaaacagt agtcggtgag ttgggagata agactaaagg
     3901 cttccaattt gaacttgagt tgaaagataa aactggacag cctattgtcg acgctctaaa
     3961 aactaataat caagatttag tagctaaaga tgggaaatat tcatttaatc taaagcatgg
     4021 tgacaccata agaatagaag gattaccgac gggatattct tatactctga aagagactga
     4081 agctaaggat tatatagtaa ccgttgataa caaagttagt caagaagctc aatcagcaag
     4141 tgagaatgtc acagcagaca aagaagtcac ttttgaaaac cgtaaagatc ttgtcccacc
     4201 aactggtttt actactgatg gtggaaccta tctgtggtta ttattgcttg tcccatttgg
     4261 tttgttagtg tggttctttg gtcgtaaagg actaaaaaat gactaattac ctaaatcgct
     4321 taaatgagaa tccactattt aaagctttca tacggttagt acttaagatt tctattattg
     4381 gatttctagg ttacattcta tttcagtatg tttttggcgt catgattgtt aacacaaatc
     4441 agatgagtcc tgctgtaagt gctggtgatg gagtcttata ttatcgtttg actgatcgct
     4501 atcatattaa tgatgtggtg gtctatgagg ttgataacac tttgaaagtt ggtcgaattg
     4561 ccgctcaagc tggcgatgag gttaatttta cgcaagaagg aggactgttg attaatgggc
     4621 atccaccaga aaaagaggtc ccttacctga cgtatcctca ctcaagtggc ccaaattttc
     4681 cctataaagt tcctacgggt acgtatttca tattgaatga ttatcgtgaa gaacgtttgg
     4741 acagtcgtta ttatggggcg ttacccatca atcaaatcaa agggaaaatc tcaactctat
     4801 taagagtgag aggaatttaa aaaaatttgg agagagagaa aatgaaaaaa aataaattat
     4861 tacttgctac tgcaatctta gcaactgctt taggaacagc ttctttaaat caaaacgtaa
     4921 aatctgagac ggcaggagtg tccgaaaatg caaaattaat agtaaaaaag acatttgact
     4981 cttatacaga caatgaagtt ttaatgccaa aagctgatta tacttttaaa gtagaggcag
     5041 atagttctgc aactgataaa acgaaagatg gtttagagat taagccagga gtaactgaag
     5101 gtttaactac cgagcagaca attgcttatg ataactcagt taaaccatcg gataaatcta
     5161 agactgcaac ttttgatttt tcaaccgtaa aatttccaga agttggtgtt tatcgttata
     5221 cggtttcaga aatagatagc aaagttagtg ggattaaata cgataccaaa acatggatag
     5281 tggatgttta tgttgttaat gatggtaatg gaggatttaa ggcacggtat attgtatcta
     5341 aagagaaggg acaaaatgat aaaaagcctg ttgtttttga aaactccttt aagacaactt
     5401 ctttaaaggt agaaaaacag gtgactggta atacaggaga actaaagaaa gacttcaatt
     5461 tcactttgac aattaatcct aatgataatt tcgtagctgg acaagttatt aaattagaga
     5521 aaggtggaat taaagctgat gttaagattg gtgagccgta taaatttgct ctaaagaatg
     5581 gtgagaaagt aactttatca aaattaccag taggtgttac ttatagcatc attgaagatg
     5641 aggcagataa agacggatac acaactaatg ccaaaattac agatggtacg gcagcgcctg
     5701 tggaatataa gttaggaaat caacaattgg ctgacgaatc tgctgacgaa atcgttgtca
     5761 caaataaccg tgacactcaa gttccaactg gtgttgtagg cacccttgct ccatttgcag
     5821 ttcttagcat tgtggctatt ggtggagtta tctatattac aaaacgtaaa aaagcttaag
     5881 tgattagtca aagaatgatg atgacaattg tacaggttat caataaagcc attgatactc
     5941 tcattcttat cttttgttta gtcgtactat ttttagctgg ttttggtttg tgggattctt
     6001 atcatctcta tcaacaagca gacgcttcta atttcaaaaa atttaaaaca gctcaacaac
     6061 agcctaaatt tgaagacttg ttagctttga atgaggatgt cattggttgg ttaaatatcc
     6121 cagggactca tattgattat cctctagttc agggaaaaac gaatttagag tatattaata
     6181 aagcagttga tggcagtgtt gccatgtctg gtagtttatt tttagataca cggaatcata
     6241 ataattttac ggacgattac tctctgattt atggccatca tatggcaggt aatgccatgt
     6301 ttggcgaaat tccaaaattt ttaaaaaagg attttttcaa caaacataat aaagctatca
     6361 ttgaaacaaa agagagaaaa aaactaaccg tcactatttt tgcttgtctc aagacagatg
     6421 cctttgacca gttagttttt aatcctaatg ctattaccaa tcaagaccaa caaaggcagc
     6481 ttgttgatta tatcagtaaa agatcaaaac aatttaaacc tgttaaattg aagcatcata
     6541 caaagttcgt tgctttttca acgtgtgaaa atttttctac tgacaatcgt gttatcgttg
     6601 tcggtactat tcaagaataa cgaaaggagg agacttttga gaaaatattg gaaaatgtta
     6661 ttttctgtcg taatgatatt aaccatgctg gcctttaatc agactgtttt agcaaaagac
     6721 agcactgttc aaactagcat tagtgtcgaa aatgtcttag agagagcagg cgatagtacc
     6781 ccattttcga ttgcattaga atcaattgat gcgatgaaaa caatagaaga aataacaatt
     6841 gctggttctg gaaaagcaag cttttcccct ctgaccttca caacagttgg gcaatatact
     6901 tatcgtgttt atcagaagcc ttcacaaaat aaagattatc aagcagatac tactgtattt
     6961 gacgttcttg tctatgtgac ctatgatgaa gatgagactc tagccgcaaa agttatttct
     7021 cgaagggctg gagacgaaga aaaatcagcg attactttta agcccaaacg gttagtaaaa
     7081 ccaataccgc ctagacaacc tgacatccct aaaaccccat taccattagc tggtgaagta
     7141 aaaagtttat tgggtatctt aagtatcgta ttactgggat tactagttct tctttatgtt
     7201 aaaaaactga agagtaggct ataaaaccca tttttaagag tcacacattt ttagttgctc
     7261 tatttttata atgaaactta ttgacatctc catagtcttg cttgactttg gagatgtttt
     7321 ctatgctgat aggtttttgt accatataag ataatgattg atgaaaagag tgtcttgaat
     7381 tcttaaatat tatctcggta tttagcaaga tagtcttttg aagaaatacc cgtgtatttt
     7441 ttaaagattt tatgaaaatg ggtagtgtca taaaaatata agcttttagc cacttctcca
     7501 acaggaattc ctcgttttaa gagtagttga gcttctttga tttttgtact tagaatataa
     7561 tgttgtaagg aaacatttga gtatttttta aagactgaac gtaagtgaga ttcggaaaca
     7621 tataggcgct tagcgatatc agataccttt aatttggaat acaggtgact atcgacataa
     7681 tgaagcacac tattgtacat ttgacgttta tcagaaatac ttttattggt taatgattcg
     7741 gaaaaatgga tgatagctgc acagcgaatt ctcaaaactt tgagcaagtc actcgcttct
     7801 tcatttaagg ccatgtagta tttgacaagg ctataatgct ttgctacgtc agtattttct
     7861 acaaaactga attccagtaa cctagttaaa tagataacag tgaggttctt ttccgccctt
     7921 agagcagaaa tagaagatga ggtgatactt gataagggga tgcgattgat ttcttgcttg
     7981 agcaattgtg gattgcctag ctttaccaga tccataatat gatttaggtg ctctaaaaca
     8041 ggcggtaatt ggctagatgc tttgctaata tgcttaaggc gatgaatgat atcaggatca
     8101 gcagtcatct gttttgaatg ctgcaacaat tgatgaattg tttgttggca agtcgtctca
     8161 aattgtgtgt caaaaaaagc gtcaatagct atcaaaaggt tccgcacatt attaattgga
     8221 aaatgtggga caagtgccat ataggacaag agctcttctt tgctacgatg agaagaatgt
     8281 tttaaaaaat tattagctaa ttgttcctga tagtctttat taagtgaata agggtagaaa
     8341 ggtccaatgg caataatgta gtgacaaaga ggaaaaatca taaaggactc ttcaaataaa
     8401 ccctcataaa aaataacatc ttgtgcggct tgaggaatag ctagatgctt taaaaattga
     8461 tagtaacata ataaatagtc gtcattacca tatacttgaa taagtgcctt atcttgtgac
     8521 atcactgaaa taggtaattg agacaagctg tgtaatgttt gcacatattt taaatcgaat
     8581 ataaccattt gatagattgc cccccatcct tagttgtatc attttcctta gacttgtaaa
     8641 agctttagta aatgttaatg atgaaaattc ctatttggta ggcacactaa aaggttttcc
     8701 taatagtaaa ctctctctcc atcatctcct accttaatta tactataaat agctaagcag
     8761 ataaagataa aggatttgta cgttaagcgc ttgaaaaaga aagagttaca gataatgaca
     8821 taaaaaacgc taaaaaacca tcaaaataaa tactctgacc ataagatgta gacttgacaa
     8881 ctgaaaatgg taaaataact atttgacagt tgtcctgtag tctttagttt tggacatagg
     8941 ctgtcgctta tgaatgtgga gagagaaaat aaatgacaca aaaaaatagc tataagttaa
     9001 gcttcctgtt atccttaaca ggatttattt taggtttatt attggttttt ataggattgt
     9061 ccggagtatc agtaggacat gcggaaacaa gaaatggagc aaacaaacaa ggatcttttg
     9121 aaatcaagaa agtcgaccaa aacaataagc ccttatcggg agcaactttt tcactgacac
     9181 caaaggatgg gaagggaaaa cctgttcaga cctttacctc aagcgaagag ggtattatag
     9241 atgctcaaaa tctccaacca gggacttata ccttaaaaga agataaagca ccagatggtt
     9301 atgataaaac cagtcggact tggacagtga ctgtttatga gaacggctat accaagttgg
     9361 ttgaaaatcc ctataatggg gaaatcatca gtaaagcagg gtcaaaagat gttagtagtt
     9421 ctttacagtt ggaaaaatca acaatgtctg tattctctaa atacggaggg acagaagtaa
     9481 ctagtggcgc agcggatttc taccgcaatc atgccgccta ttttaaaatg tcttttgagt
     9541 tgaaacaaaa ggataaatct gaaacaatca acccaggcga tacctttgtg ttacagctgg
     9601 acaaacgtct caatcctaaa ggtatcagtc aagatatccc taaaatcatt tatgacagtg
     9661 aaaatagtcc gcttgcgatt ggaaaatacg atgccaagac tcatcagctc acctatactt
     9721 ttacaaatta tatcgctggt ttagataaag tccagttgtc tgcagaattg agcttattcc
     9781 tagagaataa ggttgtttta gaaaatcaaa cgatatcaga ttttaagagt accataggtg
     9841 atcaggaaat caaatatggt gggacagtta atgtccttta tggaaatgca tctgggcaag
     9901 acaacaatta tgttactaac ggattgagca atgtgggtgg aagtattgaa agctacaata
     9961 cacaaacagg cgactttact tggtatgttt atgtcaaccc aaatcgtacc aatattcctt
    10021 atgcgaccat gaatttatgg ggatttggaa ggttggttaa atctaataac tcaacaagta
    10081 agtcagagga ttccgctaca agtagtgccc aattggaaaa agtagaaatt tatgaagtta
    10141 aagaggacta tacgctaccc actagttatg gggttgatct aacaaaattg aatttaagaa
    10201 aagaccttga ggcaaagtta ccacaagata gtactcaaaa ttctaataag cgcttgcgaa
    10261 ttgactttgg agataaactt caaaataaag cctttgttgt taaaataaca gggaaaacgg
    10321 atcaatctgg taagccacta gtcgttcaat ccaatttggc cagttttaat aatgaaactg
    10381 ctgtaactta tggtaatcct gttaataacg tctacttcca aaacgaaatt gccttgtctc
    10441 cttctaaggg tagtggttct gggacaagtg aatttactaa gccctctatt acagtagcaa
    10501 atctaaaacg agtggctcag cttcgcttta agaaaatgtc aactgacaat gtgccattgc
    10561 cagaagcggc ctttgagctg cgttcatcaa atggtaatag tcaaaaagta gaagcaaaat
    10621 caaacacaca aggagaggtt cattttaagg acctgacctc gggcacctac gacttgtatg
    10681 aaaaacaagc acctaaaggc tatcagcagg tgacagagaa attggcgacc gttactgttg
    10741 atactaccaa acctgctgag gaaatggtca cttggggaag cccacattcg tctgtaaaag
    10801 tagaagatag caaagaagtc acgattgtca accataaaga aacccttacg ttttcaggga
    10861 agaaaatttg ggagaatgac agaccagatc aacgtcctgc aaagattcaa gtgcaactgt
    10921 tgcaaaatgg tcaaaaaatg tccgataaag ttcaagaagt aacgaaggat aacgattggt
    10981 cttatcactt caaagacttg cctaagtacg atgacaggaa tcaggagtat aagtactcag
    11041 ttgaagaagt gaaagttcca gacggctaca aggtgtcgta tttaggaaat gatatattta
    11101 acaccagaga aacagaattt gtgtttgaac agaataactt taaccttgaa tttggaaatg
    11161 ctgaaataaa aggtcaatct gggtcaaaaa tcattgatga agacacgcta acgtctttca
    11221 aaggtaagaa aatttggaaa aatgatacgg cagaaaatcg tccccaagcc attcaagtgc
    11281 agctttatgc tgatggagtg gctgtggaag gtcaaaccaa atttatttct ggctcaggta
    11341 atgagtggtc atttgagttt aaaaacttga agaagtataa tggaacaggt aatgacatca
    11401 tttactctgt tagagaagta actgttccaa caggttatga tgtgacttac tcagctaatg
    11461 atattattaa taccaaacgt gaggttatta cacaacaagg accgaaacta gagattgaag
    11521 aaacgcttcc gctagaatca ggtgcttcag gcggtaccac tactgtcgaa gactcacgcc
    11581 cagttgatac cttgtcaggt ttatcaagtg agcaaggtca gtccggtgat atgacaattg
    11641 aagaagatag tgctacccat attaaattct caaaacgtga tattgacggc aaagagttag
    11701 ctggtgcaac tatggagttg cgtgattcat ctggtaaaac tattagtaca tggatttcag
    11761 atggacaagt aaaagatttc tacctgatgc caggaaaata tacatttgtc gaaaccgcag
    11821 caccagacgg ttatgagata gcaactgcta ttacctttac agttaatgag caaggtcagg
    11881 ttactgtaaa tggcaaagca actaaaggtg acgctcatat tgtcatggtt gatgcttaca
    11941 agccaactaa gggttcaggt caggttattg atattgaaga aaagcttcca gacgagcaag
    12001 gccattctgg ctcaactact gaaatagaag acagtaaatc ttcagacctt atcattggcg
    12061 gtcaaggtga agttgttgac acaacagaag acacacaaag tggtatgaca ggccattctg
    12121 gctcaactac tgaaatagaa gacagcaagt cttcagacgt tatcattggc ggtcaggggc
    12181 aggttgtcga gacaacagag gatacccaaa ctggcatgca cggggattct ggttgtaaaa
    12241 cggaagtcga agatactaaa ctagtacaat ccttccactt tgataacaag gaatcagaaa
    12301 gtaactctga gattcctaaa aaagataagc caaagagtaa tactagttta ccagcaactg
    12361 gtgagaagca acataatatg ttcttttgga tggttacttc ttgctcactt attagtagtg
    12421 tttttgtaat atcactaaaa accaaaaaac gcctatcatc atgttaagat aagctgttgg
    12481 tgacaatagc gaaaaagggt ccacttgtta gtggacctct ttttctggaa tcgatcgtct
    12541 cggttcacta gataagtcat tttaagtagt tcaaaaaggc agagccttaa aaagttttct
    12601 gcctttttaa tcttgtgggg cacttacgaa agtatttcgg ccacttcaag cataataaaa
    12661 agagtctgag caatcatgtt gtctcagact ctgactgtaa ttatgcaaaa gatgatggac
    12721 tggcagcttc atttactcac cgaactcccc tttataactt gggaatgatg ctgctaatgg
    12781 gttgttttgt ttaatacctg ctataaacgg taatttatcc catttatctt tgtaattatc
    12841 ttcaagctca gttttaaact tccaaaccat tttatgagaa aaataagtgt gcgattgacg
    12901 gccgtaggtg tctactaaaa cgtgagaaaa ttttccgtcg ttttttagtt tgtaaaattt
    12961 atctagagaa ggactccagc cagtataacc agggaattta tcaaattctt gattaaaatt
    13021 tgtttgttgg cttccccaat cataatgcca ttgtgtatta ttgtgttttt tagaatcaac
    13081 tgtaccgttc ccataatgat gctcataagt tatctccgta ggagctttta cacccttagg
    13141 aagaaacact gagaaatcag ggtaaaccca ttgttcttta gctgaattaa aggttacagt
    13201 ccattcgata ctgttattgt tatcgaccaa tgttttagta tagtcgacat agttatcaac
    13261 ctagtaattt ccctgccagt aaaggtgcac tttacctttt tcatcaatgt tatcatgggc
    13321 atgtactgat ttatacgaac taaacagcat cgaagctgct agtagtgaag tgaagagata
    13381 tttattacat cgtttcatat catgaaaaac c
//
LOCUS       DATXQK010000041        13244 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.041, whole genome shotgun sequence.
ACCESSION   DATXQK010000041 DATXQK010000000
VERSION     DATXQK010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13244
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.041"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..724)
                     /locus_tag="VC394_001345"
     CDS             complement(<1..724)
                     /locus_tag="VC394_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HEP5994810.1"
                     /translation="MLTSKHHNLNKLVWRYGLTSAAAVLLAFGGGASSVKAEVSTAAM
                     TKEERQQEIEKIKEDIKKEYPKASEEKFWESSFWGRRYFNEQEYLKSLQSFTEQRLQD
                     IVNLIEKGATKGDKGDAGPKGERGPAGPQGPVGPKGEAGKVGAQGPKGDPGAPGKDGA
                     KGEKGDKGDTGERGEKGDIGATGAQGPAGPQGEAGKPGEQGPAGPQGEAGKPGEQGPA
                     GPQGEAGKPGEKAPEKSPEGEAG"
     gene            1106..1588
                     /gene="nrdI"
                     /locus_tag="VC394_001346"
     CDS             1106..1588
                     /gene="nrdI"
                     /locus_tag="VC394_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEP5994811.1"
                     /translation="MPQITLVFISLSGNTLSFVKRLSLYLADNYDYHVKQINIKDLKH
                     ETFPVKEEFVAILPTYLEGGNGVDSGEVEILTTPLGEFIAAHGNAQRCLGIIGSGNKN
                     FNHQYCLTAKQYAKRFGFPLLGDFELRGTPDDISRLAQVIMEASSRHSSNDTQTLPNS
                     "
     gene            complement(1982..2800)
                     /locus_tag="VC394_001347"
     CDS             complement(1982..2800)
                     /locus_tag="VC394_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEP5994812.1"
                     /translation="MTKVLTLNTHSWMQANTLKKLVALAEHILAEKYDIICLQEINQL
                     IESELATDLPRYQALSGTPNIHKDHFALLLIHYLQKRGQHYYWSWAYNHIGYDIYQEG
                     VAILSKQPIHVSDILVSAMADETDYHTRRSLIAKTTLDGKEVAVVNVHLSWFDKGFLG
                     EWEKLEKELLTLNCPLLLMGDFNNPTDQDGYQVMMGSPLDLQDSHKVADHVFGDHSIV
                     ADIDGWQGNKEALKVDHVFTSKDFIIRSSKITFEGGDAPVVSDHYGLEVTLGWR"
     gene            complement(2883..5069)
                     /locus_tag="VC394_001348"
     CDS             complement(2883..5069)
                     /locus_tag="VC394_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEP5994813.1"
                     /translation="MKTSFKQLFRFEFWQKFGKCLMVVIAVMPAAGLMISIGNSIPMI
                     NHESAFLASLGNIIAQIGWAVIVNLHLLFALAIGGSWAKERAGGALASGLAFVLINRI
                     TGAFYGVSSAMLADPEAKITSLLGTQMIVKDYFTSVLESPALNTGVFVGIIAGFVGAT
                     AYNKYYNYRKLPEVLTFFNGKRFVPFVVILRSIFVALILVVVWPVIQSGINSFGMWIA
                     SSQDSAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYEVMTGAAAGTKVFGQ
                     DPLWLAWVTDLVHLKGSDASAYSHLMDSVTPARFKVGQMIGATGTLMGVALAMYRNVD
                     ADKKHTYKMMFISAAAAVFLTGVTEPLEYLFMFAAMPLYIVYALVQGASFAMADLVNL
                     RVHSFGNIELLTRTPMALKAGLGMDVINFVWVSILFAVIMYFIADMMIKKMHLATAGR
                     LGNYDADILGDHNTQATPTQVADSNSQVVQIINLLGGAGNIDDVDACMTRLRVTVKDP
                     AKIGAEDDWKKAGAMGLIQKGNGVQAVYGPKADILKSDIQDLLDSGAVIPEVNMSQLT
                     SKPTPAKDFKHVTEEVLSVADGMVLPITGVKDQVFAAKMMGDGFAVEPTHGNIYAPVA
                     GLVTSVFPTKHAFGLLTDNGLEVLVHVGLDTVALNGVPFSVKVSEGQRVHAGDLLVVA
                     DLAAIKSAERETTIVVAFTNTTEIQDVTLTSLGAQPAKTKVATVEL"
     gene            complement(5426..6175)
                     /locus_tag="VC394_001349"
     CDS             complement(5426..6175)
                     /locus_tag="VC394_001349"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEP5994814.1"
                     /translation="MQQYFIKGKAEKEVTITDKDTIKHMFQVMRLADKAEVVLVFDDG
                     VKYLAKVTNSMAHELEIIEALPDQVELPVKVTIASGFPKGDKLDTIAQKVTELGASAL
                     WGYPADWSVVKWDGKKLAKKEDKLAKIVLGAAEQSKRNRVPEVHLFEHKAEFLKSLSG
                     FDHIFVAYEETAKAGQLATLAREVKEVKPGAKILFIFGPEGGISPTEITQFEAAGAIK
                     VGLGPRIMRAETAPLYALSALSYALELQASK"
     gene            complement(6175..7128)
                     /locus_tag="VC394_001350"
     CDS             complement(6175..7128)
                     /locus_tag="VC394_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011055040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEP5994815.1"
                     /translation="METWQEVTVHVHRDAQEAVSHVLIETGSQGVAIADSADYIGQKD
                     RFGELYPDVEQSDMIAIIAYYPSSTNLADVIATINEQLAELASFGLQVGQVTVDSQEL
                     AEEDWADNWKKYYEPARITHDLTIVPSWTDYDASAGEKVIKLDPGMAFGTGTHPTTKM
                     SLFALEQILRGGETVIDVGTGSGVLSIASSLLGAKTIYAYDLDDVAVRVAQENIDLNQ
                     GTDNIHVAAGDLLKGVSQEADVIVANILADILVLLTDDAYRLIKKEGYLILSGIISEK
                     LDMVLEAAFSAGFFLETHMVQGEWNALVFKKTDDISGVIGG"
     gene            complement(7198..7668)
                     /locus_tag="VC394_001351"
     CDS             complement(7198..7668)
                     /locus_tag="VC394_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEP5994816.1"
                     /translation="MAKYGFLSVLEEEMDKHFQYDYAMDWDKKNHAVEVTFVLEAQNK
                     EAIKTIDDSGEVTQDDIVFEDYVLFYNPAKSQFDAADYLVTIPFDAKKGFSREFLAYF
                     AQFLNDVAIEGHSDLMDFLADDSKVDFGLEWNAQAFEEGQQGLEEAASYAYPRY"
     gene            7869..9626
                     /gene="pabB"
                     /locus_tag="VC394_001352"
     CDS             7869..9626
                     /gene="pabB"
                     /locus_tag="VC394_001352"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEP5994817.1"
                     /translation="MHRKTIIDFKELGQRYLFDEPLVELVAKSLDQVGPVIENVQHYQ
                     QLGYYVVGYLSYEAAAFFDNALQTHNDRLGNEYLAYFTVHKTCQKKDLPLDYDGITIP
                     NQWVSATQKEAYQKAIETIHREMQQGNTYQVNYTLQLTQELNAADSLAIYNKLVVEQA
                     AGYNAYIAHDDFAVISASPELFFKQEGNRLTTRPMKGTTKRGVNSWLDQQEHDWLQAD
                     GKNRSENMMIVDLLRNDMGKICQTGSVCVDRLCEVERYSTVWQMTSTIVGDVKADCDL
                     IDILTALFPCGSITGAPKVSTMAIITSLEPKPRGIYCGSIGICLPDGRRFFNVPIRTI
                     QLSHNQATYGVGGGITWQSKWEDEYEEVHQKTAFLYRHKQIFDLKTTAKVKHKKIAFL
                     EQHLNRLKEAATYFAYPYDEKALQKQLSTYLENKDNAAYRLMIRLSKDGKISLSDQPL
                     EPLSADFLTAQLSLQKKDVTASPFTYFKTSYRPHIEQKSYEQLFYNQAGQLLETSIGN
                     LFVQLGRTLYTPPVVVGILPGLFRQELLATGQAQEKEVTLADLKEASAIFGGNAVRGL
                     YPLNLKLTHLDALLAKSQA"
     gene            9659..10225
                     /locus_tag="VC394_001353"
     CDS             9659..10225
                     /locus_tag="VC394_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEP5994818.1"
                     /translation="MILLIDNYDSFTYNLAQYLSEFDETIVLYNQDPNLYDMAKKANA
                     LVLSPGPGWPKEANQMPKLIQDFYQTKPILGVCLGHQAIAETLGGNLRLAKRVMHGRQ
                     STIETQGPASLFRSLPQEITVMRYHSIVVDQLPKGFSVTARDCDDQEIMAFEHHTLPL
                     FGLQFHPESIGTPDGMTMIANFIAAIPR"
     gene            10258..11526
                     /locus_tag="VC394_001354"
     CDS             10258..11526
                     /locus_tag="VC394_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEP5994819.1"
                     /translation="MPDHLALRMRPKTISEVIGQKHLVGEGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTRYAFRTFNATIDSKKRLQEIAEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLENGTIIMIGATTENPFFSVTPAIRSRVQIFELEPLSNEDIKTAIQ
                     LAISDKERGFPFLVTIDDEALDFIVTATNGDLRSAYNSLDLAVMSTSPNEDSSRHISL
                     ETMENSLQRSYITMDKNGDGHYDVLSALQKSIRGSDVNASLHYAARLVEAGDLPSLAR
                     RLTIIAYEDIGLANPDAQVHTVTALDAAQRIGFPEARILIANVVIDLALSPKSNSAYL
                     AMDAALADLRTSGNLPIPRHLRDGHYAGSKDLGNAKDYLYPHAYPEKWVKQQYLPDKL
                     VGHHYFEANETGKYERALGSNKERIDKLSD"
     gene            11615..11811
                     /gene="ssrS"
                     /locus_tag="VC394_001355"
     ncRNA           11615..11811
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VC394_001355"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            11812..11884
                     /locus_tag="VC394_001356"
     tRNA            11812..11884
                     /locus_tag="VC394_001356"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11845..11847,aa:Lys,seq:ctt)
     gene            12025..12465
                     /locus_tag="VC394_001357"
     CDS             12025..12465
                     /locus_tag="VC394_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP5994820.1"
                     /translation="MKIIEYKGNYRDDLIFMVLEAKNALRRIPSINPDLLTIETSYLE
                     KGDCFWLAVNEESRVIGSIAYSSIPKTNEVFLHRLFIKPSLKHQGIGSQLLTFAEDYL
                     RKIGKSHVRIHLGQPKEQWFESYRFYPKHGYQFYDDTHLIKKLI"
     gene            12520..13026
                     /locus_tag="VC394_001358"
     CDS             12520..13026
                     /locus_tag="VC394_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEP5994821.1"
                     /translation="MKIAIIGHSGSGKSTLARFLGQHYHCEVVHLDQLHFSSNWQERS
                     DHDMIADLSTCLLKQDWIIEGNYANCLYEERMSEANHIIYVNFSRFHCVYRAFKRYLN
                     YRGKTRPDMADNCQEKFDVAFVKWILLDGRSRNQLKKYQSVIQKYPHKTIVLTNQKQL
                     NHYMNTIN"
BASE COUNT         3706 a         3081 c         2602 g         3855 t
ORIGIN      
        1 ggcctgcttc gccttctggg ctcttttctg gagctttttc acctggtttg cctgcttcac
       61 cttgtgggcc tgctggacct tgctcacctg gtttgcctgc ttcaccttgt gggcctgctg
      121 gaccttgctc acctggtttg cctgcttcac cttgtgggcc tgctgggcct tgtgcgccag
      181 ttgctccgat gtcacctttt tcaccgcgct caccggtatc acctttgtcg cctttttcac
      241 ctttggcgcc gtctttacct ggggcaccag gatcaccttt aggaccttgg gcaccaacct
      301 tacctgcttc acctttaggt cctaccggac cttgtgggcc tgctgggccg cgctcgcctt
      361 taggaccagc atcgcctttg tcaccttttg ttgcaccttt ttcaatcaga tttacaatat
      421 cttggagtct ttgctcagta aaactttgta aagattttag atactcttgt tcattaaagt
      481 accttcttcc ccaaaagcta ctctcccaaa atttttcctc agacgctttt ggatattctt
      541 tcttaatgtc ttctttaatt ttctcaattt cttgttgtct ctcctccttc gtcatagccg
      601 cagtagaaac ctcagcctta acgctgcttg ccccgcctcc aaaggctaga aggacggcag
      661 cggctgaggt tagcccgtag cgccagacta gtttgttgag attgtggtgt tttgatgtca
      721 acatatgttg ttctctcttt ctttattaga tggtgtgttt tagataaaaa taacatggta
      781 gctagacctg attatttata atagtattat aatagagtgt gaaaacgatt gtaaagttct
      841 cttttgttgt tcatctttta gattttgact gatcactctt ttgtggagat cagagggcta
      901 ctttggcact tgcctgcctc gtttttaagt cgagggcatc cggatccttt ctttaaggtc
      961 actctttttt gaaatgttac tttttttgac cgcttgtacc ttcttgtctg gttggatatc
     1021 gctaggtttc ttctttactt gtttgttggt cttgattgtg gtaagcttat gaagaaacct
     1081 tgtaggataa aaaaaggaga cacttatgcc tcagattact cttgttttta ttagcctcag
     1141 tggcaacacc cttagctttg tcaagcggct atccctttat ttagcagaca attacgatta
     1201 ccatgtcaaa cagattaaca ttaaagattt gaagcacgag acttttcccg tcaaggaaga
     1261 gtttgtggcg attctaccca cttatctaga aggcggaaat ggcgttgact ctggtgaggt
     1321 ggagatcctc accactcctt taggagagtt tattgccgcc catggcaacg cccagcgttg
     1381 tcttgggatt attggttcag gcaacaaaaa ctttaaccat cagtattgcc tgacagccaa
     1441 acagtatgcc aaacgctttg gctttccact gctaggtgat tttgagctgc gagggactcc
     1501 tgatgacatt tcgcggttag cccaggtcat catggaggcc agctccagac actcgtctaa
     1561 cgacacacag acactgccta acagctagac cgtctataga tgatcatagc tgttttaaaa
     1621 aagagcactt ttaggacacc aactagggag gtctcttacc ctacgccttg cgatttctca
     1681 gcctttgaaa agagcgatca cagtgcttct tggggcttgg gatcactcca agcgctccta
     1741 gaaatacccc tatgcagact ttagcaaggc aactgaacgc ttggcggtac tttctgtgaa
     1801 gctcagatgg ttggtggcaa aaaagccctg gttttttagg gttactgaca tgactaaaga
     1861 ggtctaacca gctctacctt gagctcgtta gacctctttg tgttctccta atccccctag
     1921 acgatcgcag tttcttatgt gtcttggctt ctcctatgtg gtgtgcctgt tagcacatgc
     1981 tctaacgcca gcctaatgtc acttctaaac cgtagtggtc actgacgact ggcgcatctc
     2041 cgccttcaaa ggtaatctta gaagatcgga tgatgaagtc cttgctggta aagacatggt
     2101 ccaccttgag ggcttctttg ttaccttgcc agccatcaat atcagccacg atgctatgat
     2161 caccaaacac atggtcagca accttatggc tatcttgtag gtctaggggg ctacccatca
     2221 tcacttgata accgtcttga tccgttggat tgttaaaatc tcccataagt agcaagggac
     2281 agtttagtgt gagcagttct ttttctaatt tctcccactc ccctaaaaac cctttgtcaa
     2341 accaagatag gtgcacattg acaacagcga cttctttgcc atctaacgtg gttttggcta
     2401 tgagtgagcg tctggtgtgg tagtctgttt catcagccat cgctgacacg agaatatctg
     2461 aaacatgaat aggttgtttt gataagattg ccacgccttc ctggtaaatg tcatagccaa
     2521 tatggttata agcccaagac caataatagt gctgccctcg tttttgtaaa taatggatca
     2581 agagcagggc aaaatggtct ttatgaatgt ttggtgtccc tgaaagagct tgatatcttg
     2641 gtaaatctgt ggccagctcg ctctcgatta actgattgat ttcttgcaag caaatgatat
     2701 cgtatttttc agctaaaata tgctcagcca aagcaactaa ttttttaaga gtgttggctt
     2761 gcatccacga atgggtattc agtgttaata ccttggtcat taatcctcct ttataagaca
     2821 agaagagcca tagggcctaa accagctatg gctattacct atcactatct cgtgatgttt
     2881 tgttaaagtt ctacggtagc tactttagtc ttagcaggct gagctcctaa agatgtcaag
     2941 gtcacatctt ggatctctgt tgtgttggta aaagcaacaa caatggttgt ttcacgctct
     3001 gctgatttaa tagcagcaag atctgctacg actaataggt cacctgcatg aacccgttgt
     3061 ccttctgaga ctttgactga aaaaggcaca ccatttaagg cgacggtatc aagtcccacg
     3121 tgcaccagca cttcaagacc attatctgtc agtaaaccaa aggcatgctt ggtcggaaag
     3181 acactggtca caaggccagc tactggtgca tagatattac catgggttgg ttcaaccgca
     3241 aacccatctc ccatcatctt agccgcaaac acctggtctt tcacacctgt gattgggaga
     3301 accatcccgt ctgccactga tagcacttct tctgtcacgt gtttaaaatc ttttgcggga
     3361 gtcggtttgc tagtgagctg tgacatgttg acttctggaa taacagcccc tgagtccagc
     3421 aaatcttgaa tatctgattt caaaatatct gctttaggcc catagaccgc ttgaacgccg
     3481 ttgccttttt ggatcaagcc catagcgcca gcttttttcc aatcgtcctc agcgccaatc
     3541 ttagcggggt ctttgaccgt cactcgtaag cgcgtcatac aagcatcaac atcatcaata
     3601 ttgcctgccc caccaaggag attaatgatt tgtacgactt gagagttgct atctgctact
     3661 tgggtaggtg ttgcttgagt gttatggtca cccagtatat cggcatcgta gttgcctaaa
     3721 cggccagctg tcgcgagatg cattttcttg atcatcatat ccgcaataaa gtacatgata
     3781 actgcaaaga ggatagaaac ccaaacaaag ttaatcacat ccatgcctag acctgcttta
     3841 agggccatag gggtacgggt tagtagctca atgttcccaa atgagtgaac acggagattc
     3901 acaaggtctg ccatggcaaa tgaagctcct tgaacaaggg catagacgat gtaaagtggc
     3961 attgctgcaa acataaatag gtactcaagt ggctcagtta cccctgtcaa aaagaccgct
     4021 gccgctgctg agatgaacat cattttgtat gtatgttttt tatcggcatc cacattacgg
     4081 tacatggcta aggcaacccc catcaatgtt ccggtagctc caatcatttg tcctacctta
     4141 aagcgagctg gagtcacact atccattaag tgactatagg ctgaagcgtc tgatccttta
     4201 aggtgaacaa ggtcagttac ccaagcaagc cacaaagggt cttgaccaaa tacttttgtc
     4261 cccgctgctg cacctgtcat gacctcataa gttccaccaa gagctgtata gttcattgga
     4321 atcgtcaaca tgtggtgaag accaaatggc aacaagagac gttccaaggt accatatagg
     4381 aatggggcta ggattggagc tgaatcttgt gaagaggcaa tccacatccc aaaactgtta
     4441 atcccagact ggatcaccgg ccaaacaaca accaaaataa gggctacaaa aatagaacgt
     4501 aaaataacga caaacggtac aaaacgcttc ccattaaaga aggtcaaaac ttcaggaagt
     4561 ttacggtaat tatagtattt gttataggcc gttgctccta cgaaccctgc aataatccca
     4621 acaaaaaccc ctgtgtttaa agctggagac tccaagacac tggtgaaata atctttaacg
     4681 atcatttgag tgccaaggag gcttgtgatt tttgcttctg gatcagctag catagcacta
     4741 gacacgccat aaaaagcacc tgttatccga ttgatcaaga caaaagcaag ccctgaggca
     4801 agggcccctc cagctctttc tttagcccaa ctaccaccaa tggccaaggc aaatagcaaa
     4861 tgaaggttaa caataacagc ccaaccaatt tgagcgataa tgttgcctag agatgccaaa
     4921 aatgctgatt cgtggttaat cattggaata gagtttccga tactgatcat taaaccagcc
     4981 gctggcatga cagcaataac gaccataaga catttcccaa atttttgcca aaattcgaaa
     5041 cgaaacagtt gcttgaatga tgttttcatt ctatctctcc tttattatcc ccttttagcc
     5101 atagctgccc ttaaggcgct tttgtgacac aatgatatag actgcaactc agtgatgcaa
     5161 tcgtttgcat tgatctgata cctttattat actagcctcg caacaatttg taaagcgttt
     5221 tcttagacag atgttagtcg tttttcttca ttaaatggta gtgtaactta ccaaaaagat
     5281 aacaagagcc catttccttt tttggtgatg agaccatcaa cgctcttcga aaaaacaaga
     5341 agcaataaaa aaaggccgtt gacacaatcg tgaactgcct gttcgtttct ttagcctctt
     5401 ctttctaaag taagcagcag cttacttatt ttgatgcctg caattccaaa gcataactaa
     5461 gagcacttaa ggcatatagc ggcgctgtct ctgctcgcat aatgcgaggg cctaagccca
     5521 ccttgatggc acctgctgcc tcaaactgag tgatttcagt tggggaaatt cccccttctg
     5581 gtccaaagat aaagaggatt tttgcccctg gcttaacttc ttttacttca cgcgccaagg
     5641 tcgctagctg acctgcctta gctgtttctt cgtatgcaac gaaaatatga tcaaagccag
     5701 aaaggctttt tagaaactct gctttgtgct caaaaaggtg aacttcaggc accctatttc
     5761 gtttgctctg ctcagccgct cctaatacaa tcttagccaa tttgtcttct tttttagcta
     5821 attttttgcc atcccatttg accactgacc agtctgctgg gtagccccat agcgcactgg
     5881 ctcccaattc agtcactttc tgtgcaatcg tgtctaactt atcgccttta ggaaatccag
     5941 aagcaatagt caccttaaca ggcaattcca cctgatctgg caaagcctct ataatctcta
     6001 actcatgagc catactattg gtcaccttgg ctaaatactt caccccatca tcaaagacca
     6061 agacaacttc agccttgtca gccaagcgca tcacttgaaa catgtgcttg atggtatctt
     6121 tatcggtaat tgttacctct ttttctgctt tgcctttaat aaaatactgt tgcattagcc
     6181 gccaatcact cctgagatat catctgtttt tttaaagaca agggcgttcc actcaccttg
     6241 aaccatatgc gtctcaagga agaatcctgc gctaaaggca gcttccagaa ccatatccaa
     6301 tttctcagaa atgatgcctg ataagataag gtaaccttcc tttttgatca aacggtaagc
     6361 atcatcagtc aataagacta agatatccgc caagatattg gctacaatca catccgcttc
     6421 ttggctaacc ccttttaaaa ggtcaccagc agccacatga atgttgtctg ttccttgatt
     6481 taaatcaatg ttttcttgag ccactcgaac agctacatca tctaagtcat aagcgtagat
     6541 ggttttagca cctagtaagg aactggcaat cgagaggaca cctgatccgg tccctacatc
     6601 gataactgtc tcaccaccac gaaggatttg ttctagagca aaaaggctca tcttagttgt
     6661 gggatgtgtc cctgtcccaa atgccattcc aggatctaat ttaataacct tttccccagc
     6721 agaagcgtca taatccgtcc aactagggac aatagttaaa tcatgcgtga tgcgagctgg
     6781 ttcatagtat tttttccaat tgtctgccca gtcttcctca gctaattctt gactatccac
     6841 agtaacctga ccaacctgta acccaaaact agctaactcc gctaactgtt cattaatggt
     6901 cgcaataaca tccgccaaat tagtgctact tgggtaatag gctatgatag ctatcatgtc
     6961 agactgctcc acatctgggt ataactcccc aaagcggtcc ttttgcccaa tatagtctgc
     7021 gctatccgcg atagcgaccc cttggctgcc tgtctcaatc aacacatgtg aaacagcctc
     7081 ttgcgcatca cgatgcacat gaaccgtcac ttcttgccac gtttccataa gtatgtcctt
     7141 tctattttga ccttttggtt attgtcctaa tggtgttaca ccatctcgtg tgtcacctta
     7201 gtaccttggg taagcgtaac ttgctgcttc ttctagtcct tgttgacctt cttcaaaagc
     7261 ttgcgcattc cattccaagc cgaaatcaac cttgctgtca tctgctaaga aatccatcaa
     7321 atcgctgtgc ccttcaatag caacatcgtt gagaaattgc gcaaagtaag ctaagaattc
     7381 acgagaaaag ccctttttag cgtcaaaagg aatagtaacc aaataatctg ctgcatcaaa
     7441 ttgagacttg gcagggttgt aaaagagcac atagtcctca aagacaatat catcttgggt
     7501 cacttcgcca ctgtcgtcaa tggtttttat ggcctcttta ttttgagctt ctaacacaaa
     7561 agtcacttca accgcatgat ttttcttgtc ccaatccata gcataatcat actgaaaatg
     7621 cttatccatt tcttcttcta atactgataa aaagccgtat tttgccatct ttttttatac
     7681 ctaatctttc taactgttgc tgtatagagc tttgtaagac ctcattagtt tacttacttt
     7741 gacatgctga gcgtactgtt ataactttta ttaactgaat tataacatat ttgactaata
     7801 ttgaccataa gagaaggtct taatggctgg atcacctcta gcaaccgctt ttatgatata
     7861 atttgggtat gcatagaaaa acgattattg atttcaaaga gctcgggcaa cgctatcttt
     7921 ttgatgagcc attagtagaa ttggtagcca agtccttaga tcaggtcggt ccagtcattg
     7981 aaaacgtaca acactaccaa cagctgggct attacgtggt gggctatctc agttatgaag
     8041 cagctgcttt ttttgacaat gccttacaaa cccataatga cagactaggc aacgagtacc
     8101 ttgcctattt tacagtgcac aaaacctgtc aaaaaaaaga cctacctctt gattatgacg
     8161 gcattactat ccctaaccaa tgggtcagcg caactcaaaa agaagcctat caaaaagcca
     8221 ttgaaaccat tcaccgtgag atgcaacagg gcaacactta tcaggttaac tacacccttc
     8281 aattaactca ggaactcaat gcagctgaca gcttagccat ttacaataaa ctcgtcgttg
     8341 aacaggctgc tggctacaat gcctatatcg cccatgatga ctttgctgtt atttcagcta
     8401 gtcctgagtt gttctttaaa caagagggca acaggttaac tactaggcct atgaaaggca
     8461 ccaccaagcg tggggttaat agctggctgg atcaacaaga acatgactgg ctccaagctg
     8521 acgggaaaaa ccgttctgaa aacatgatga ttgtggattt gctccgcaat gacatgggaa
     8581 aaatttgcca aacaggcagt gtttgtgtcg ataggctctg tgaggtcgag cgatactcta
     8641 ccgtctggca aatgacctca accattgttg gtgacgtcaa agcggactgt gacctgatag
     8701 acattctcac agctctcttt ccttgtggct ctatcacggg ggctcctaaa gtctccacca
     8761 tggcaattat cacatctctg gagccaaaac caagggggat ttattgtgga agtattggca
     8821 tttgcctacc tgatggtcgc cgtttcttca atgttcccat tagaaccatc cagctcagtc
     8881 ataatcaagc tacttatggt gtcggaggcg gtatcacttg gcaaagcaag tgggaagacg
     8941 aatacgagga agtccatcaa aaaacagctt ttctctatcg gcacaaacaa atctttgacc
     9001 tcaaaacaac cgccaaggtg aaacacaaaa aaatagcctt ccttgagcaa cacctcaatc
     9061 gccttaaaga agcagccact tattttgctt acccttacga tgagaaagct ttgcagaaac
     9121 aactgtcaac ctatttggaa aataaggata atgctgccta ccgcttgatg attcgtttat
     9181 ccaaggatgg aaagattagc ctctctgatc aacccttgga acctttgtca gccgattttc
     9241 tgacagctca actctcttta cagaagaagg acgtgacagc ttcgcctttt acctacttta
     9301 agaccagcta ccggcctcat attgagcaaa aatcttatga acagctattc tataaccaag
     9361 cggggcaatt attggaaacg tccattggca atctctttgt tcagctgggt cggaccctct
     9421 acacaccacc agttgtggta ggtattttac caggactttt tcgccaagag ctgctagcta
     9481 ctggtcaagc tcaggaaaaa gaggtgacac tagctgattt aaaagaggct agcgctattt
     9541 ttggaggaaa tgctgtccgt ggcctctatc ctctcaacct taagcttact caccttgatg
     9601 ccctcttagc taagagtcaa gcctaaactg ccactcatct tttgttatta aggaacctat
     9661 gatactctta attgataatt acgattcatt tacctacaac ctcgcccaat atttaagtga
     9721 atttgacgag acgattgtct tgtataacca agacccaaac ttatatgaca tggccaaaaa
     9781 agctaacgct ctagtcctct cacctggtcc tggttggccc aaggaagcca accaaatgcc
     9841 aaaactcatt caagactttt accaaacaaa acctatctta ggagtgtgtc tgggacacca
     9901 agctatcgct gaaaccctag ggggaaactt acgcttggct aaacgtgtca tgcatgggag
     9961 acaaagcacc attgaaacgc aaggccctgc tagtcttttt cgctccctgc cacaagagat
    10021 caccgtcatg cgttaccatt ccatcgttgt ggatcagtta ccaaaaggtt ttagcgtaac
    10081 tgctagagac tgtgacgatc aagaaatcat ggcctttgaa caccacaccc tgccactttt
    10141 tgggctacaa tttcacccag aaagcatcgg aactcctgat ggcatgacca tgatcgccaa
    10201 cttcatcgca gccattcccc gttaatctca tcccccaaaa gaaagaagga cagttacatg
    10261 cctgatcatt tagcgctcag aatgcgcccc aaaaccattt cagaggtcat cggacaaaaa
    10321 caccttgttg gcgaaggcaa aatcatccgt cgcatggtgg aagcaaatag actatcgtcc
    10381 atgatcctct atggccctcc aggaatcggt aaaacttcca ttgcttctgc tattgctggt
    10441 actactagat atgcctttcg gacctttaat gccaccattg acagcaaaaa acgtctccaa
    10501 gaaattgcgg aagaagctaa attttctggt ggcttggtat tattactaga cgagattcat
    10561 cgccttgaca agacaaaaca agattttctt ttacctttac ttgagaatgg caccattatc
    10621 atgattggcg caaccactga gaaccccttc ttttcagtga cacccgctat cagaagcagg
    10681 gtccagattt ttgaattgga accactttct aacgaggata ttaaaacagc cattcagcta
    10741 gcaatttccg acaaagaacg gggatttcct tttttagtga caattgatga tgaggcgcta
    10801 gatttcattg ttaccgccac aaatggagac ctacgatctg cttataattc cttggatcta
    10861 gcagtcatgt ctacttctcc taatgaagat agtagccgtc atattagtct agagacaatg
    10921 gaaaatagtc tgcagcgtag ttatattacc atggataaaa acggggacgg gcattatgat
    10981 gttttatctg ccttacaaaa atccattaga ggttctgatg tcaatgctag cctgcactac
    11041 gccgctcgtt tagtagaagc tggagacttg cctagtttag caagacgatt gaccattatt
    11101 gcttacgaag atattggctt agccaaccct gacgcccaag ttcataccgt caccgcacta
    11161 gatgccgctc aacgcattgg ttttccagaa gctcgtattc ttatcgctaa cgtggtcatt
    11221 gatttggctc tatcacctaa atccaattca gcttatttag ctatggatgc agctttagct
    11281 gatttgagaa catcaggtaa cctccccatt ccaaggcatc ttagagacgg gcattatgct
    11341 ggcagtaagg acctaggcaa tgccaaagat tacctctacc cacacgccta ccctgaaaaa
    11401 tgggtcaaac aacaatacct accagataaa ttagtaggac atcattattt tgaagccaat
    11461 gaaaccggta aatacgaacg cgcacttggt tcaaataaag agcgtattga taagctctca
    11521 gactgactgc tagttttaaa aatcgcaacc gatttcaaaa atcgataatt ttgcttgaaa
    11581 aaattttaaa aagtggtatt ataaaagtac agaaacattg ctgtggcata cgaatccaca
    11641 tctaagtgtt gaccgactat ttctttgtat tttcgggaaa caaagaccct ttctagtctg
    11701 caagccgttt cacgcggacg caacttccaa gaaagagcgc gttacccacc tgcttatact
    11761 gcgcgggctc aatacaaaaa cgtggatcac cggtgccaat acagcattct tgcctcctta
    11821 gctcagttgg tagagcagta gactcttaat ctatgggtca caggttcgag ccctgtaggg
    11881 ggtatacaac tatacatgaa aaaacctttg tttaaaggct ttttttgctt gtcagaagag
    11941 gatttgttcc atcatttatc cccgcagtaa tttttttatg gtatgatacc aattaaagcg
    12001 cttaagatta aggtggagac tgttatgaaa ataattgagt acaaaggaaa ttacagagat
    12061 gacctcattt ttatggtctt ggaagcaaaa aatgccttaa ggagaattcc cagcattaac
    12121 ccggatcttt tgacaattga aacaagttat ttggaaaaag gggattgttt ttggctagcg
    12181 gtcaatgaag aaagtcgtgt tattggctca atcgcttatt cttctattcc caaaacaaat
    12241 gaggtcttct tgcaccgtct cttcatcaaa ccaagcttaa aacatcaagg cattggcagt
    12301 caacttctga cgtttgcaga agattatcta aggaaaatcg gcaagagtca tgttcgtatc
    12361 cacttaggtc aacctaaaga acaatggttt gagtcttatc ggttttatcc taaacatggt
    12421 tatcaatttt atgatgacac gcatctcatc aagaaactaa tctaaacaca tttcataccc
    12481 tttatagtca tttcatacgt catttttagg aggtatccat tgaaaattgc catcattgga
    12541 catagtggct ctggaaagtc aaccttagca cgatttttag gtcaacatta ccattgtgag
    12601 gtggtccatt tagaccaact acacttctca tcgaactggc aagaacggtc agatcatgac
    12661 atgatagctg atttatcaac atgcctgctc aaacaagatt ggattattga ggggaattat
    12721 gccaattgtc tctatgaaga gcgcatgagt gaggctaatc acattatcta tgttaatttc
    12781 agtcggtttc attgtgtcta tcgagcattt aagcgttatt taaactatag gggaaaaacg
    12841 cgtcctgata tggctgacaa ctgtcaggaa aaatttgacg ttgcgtttgt gaaatggatt
    12901 ctactagatg gacgttcaag aaaccaatta aaaaaatacc aatccgttat ccaaaaatac
    12961 cctcataaaa ccattgtctt aacaaatcaa aagcagctca atcactatat gaatacaatc
    13021 aactaattag agtgaaaaac taaaatagcc acgggtaacc cgggctattc agactgaaag
    13081 cctttgttta aaggcttttt tgcttgtttc ataactaatc ggcttaatta aaagcgtcaa
    13141 tccactttta gtatcatgtc tcaattttca tttgatagtc agcaagtata tctacaaaat
    13201 tgttaatgat tatttctttt gaaagtgtta tattaaaaca taat
//
LOCUS       DATXQK010000042        12647 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.042, whole genome shotgun sequence.
ACCESSION   DATXQK010000042 DATXQK010000000
VERSION     DATXQK010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12647
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.042"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(124..693)
                     /locus_tag="VC394_001359"
     CDS             complement(124..693)
                     /locus_tag="VC394_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEP5994822.1"
                     /translation="MNHRKSADISRIAIFFAIMLVIHFVSSLVFNIWPIPIKPTLVHI
                     PVIIASVLYGPRIGAILGGLMGIISVITNTIILLPTNYLFSPFVDHGTFASLIIAIIP
                     RILIGITPYYCYKLIPNQFGLIVSGIIGSLTNTIFVLSGIFIFFATVFDGNIKALLTA
                     IISSNAIVEMIISAIITFVLIPTLSRLKR"
     gene            complement(751..1296)
                     /gene="coaC"
                     /locus_tag="VC394_001360"
     CDS             complement(751..1296)
                     /gene="coaC"
                     /locus_tag="VC394_001360"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEP5994823.1"
                     /translation="MTKHITLAVSGSISAYKAADLTSQLTKIGYDVHIIMTQAATQFI
                     TPLTLQVLSKNAIHLDVMDEHDPKVINHIELAKRTDLFIVAPASANTIAHLAYGFADN
                     IVTSVALALPATTPKLIAPAMNTKMYQNPITQENIKRLSTIGFTEIPPKSSLLACGDK
                     GPGALADIDVILATIDTIWKL"
     gene            complement(1289..1981)
                     /gene="coaB"
                     /locus_tag="VC394_001361"
     CDS             complement(1289..1981)
                     /gene="coaB"
                     /locus_tag="VC394_001361"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEP5994824.1"
                     /translation="MTMKLIITSGGTTEPIDAVRGITNHSTGQLGKLITERFLQYHHD
                     VTLVTTKTATKPLPNKLLRIIEVETVNDLMAALKDQVPHHDILIHSMAVSDYTPVYMT
                     DLEQVSQADNLNCFLCEHNSEPKISSASDYQVLFLKKTPKVISYVKQWNPNIKLVGFK
                     LLVNVPQEELIKVARASLAKNHADYILANDLVDIQTGMHKALLISNNEVASADTKEAI
                     ADLLYERMTKHD"
     gene            2162..3013
                     /locus_tag="VC394_001362"
     CDS             2162..3013
                     /locus_tag="VC394_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEP5994825.1"
                     /translation="MKGRIVITIRDLASLPIFVYGDGNKKVPGALSPFVWTEVFLKTI
                     NQEPNQLILHIWPMTRTVILGMLDRQLPYFELAKTEIGNNGYVPVTRNIGGLAVVADD
                     GILNFSLVIPDHFSESISISNAYLIMVDVIRESFSDYYQRIEYHEIKNSYCPGNFDLS
                     IAGRKFAGIAQRRIKKGIVVSIYLSVCGDQAARGQLIKDFYEAGTQGEVTKVNYPQID
                     PECMATLSELLETPFTVAEVLERLRLTLRQLGFSLTEKSPDQALLTNFDAVYERMQLE
                     VVRKEGK"
     gene            3010..4209
                     /locus_tag="VC394_001363"
     CDS             3010..4209
                     /locus_tag="VC394_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HEP5994826.1"
                     /translation="MKERFSPLFEPLTLPNGSQLDNRFVLSPMVTNSSTKDGYVTQDD
                     VSYALRRAASAPLQITGAAYVDPYGQLFEYGFSVTKDADISGLKELAQAMKAKGAKAV
                     LQLTHAGRFASHALTKYGFVYGPSYMQLRSPQPHEVKPLTGQQIEELIAAYAQATRRA
                     IQAGFDGVEVSSAQRLLIQTFFSTFSNKRTDSYGCQTLFNRSKLTLAVLQAVQQVINQ
                     EAPDGFIFGFRATPEETRGNDIGYSIDEFLQLMDWVLNIAKLDYLAIASWGRHVFRNT
                     VRSPGPYYGRRVNQVVRDYLRNKLPVMATGGMNTPDKAIEALAHADFIGVSTPFVVDP
                     EFAHKIKEGCEESIHLRIRPADLKSLAIPQASFKDIVPLMDYGESLPKESRTLFRSLT
                     HNYKEIK"
     gene            4206..5204
                     /locus_tag="VC394_001364"
     CDS             4206..5204
                     /locus_tag="VC394_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEP5994827.1"
                     /translation="MKVSILDYGVIDKEKTPQEALLETRCLAQVADKLGFHRFWVAEH
                     HNIYAFAISSPELLMMHLADHTKQIRIGSGGIMPLHYSSFKIAEWIMTLEALHPNRID
                     LGIGNSLGTTLVQRALSSIHCKDSYSQVVTELYQYLNPDHLSPLPIFVNPRGNTYPQI
                     WTLSNSLETAELAGQLGLGYTFGIFPYIPKDPITEAKRVSAHYRKAFRPSKLLKIPKL
                     ILAVFIVLSDTDEEAEALAKPLDIWMLGQQDFNEFKTYPDVEEARNYHLTEKQREAIA
                     ANRSRMVIGSPHTVKKQLDRLIEACQADELLAIPLVPEFANRQRTLELLADLYVTI"
     gene            5246..5578
                     /locus_tag="VC394_001365"
     CDS             5246..5578
                     /locus_tag="VC394_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEP5994828.1"
                     /translation="MKKIANYLLIEKTDDRYTISMTPELQDDIGTIGYAEFTDNDHLA
                     VDDIILNLEASKTVMSVLSPLAGAVVERNEAATLTPTLLNSEKAEENWIVVLTDVDQA
                     AFDALEDA"
     gene            5571..6383
                     /locus_tag="VC394_001366"
     CDS             5571..6383
                     /locus_tag="VC394_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HEP5994829.1"
                     /translation="MPSSFDLLGEMIDLLQTEQLTSSWACPLPNALTKRQDLWRALIN
                     QRPALPLSKDYLNLEDAYLDDWRASFVPVSVKDCQKTNYTSLFLYHGDIRYLAVDAIV
                     NAANSELLGCFIPNHGCIDNAIHTFAGSRLRLACQAIMTEQGRKEAIGQAKLTSAYHL
                     PASYIIHTVGPRITKGRHVSPIRADLLARCYRSSLDLAVKAGLTSLAFCSISTGEFGF
                     PKKEAAQIAIKTVLKWQAEHPESKTLTIIFNTFTSEDKALYDTYLQKENNCE"
     gene            6376..7257
                     /locus_tag="VC394_001367"
     CDS             6376..7257
                     /locus_tag="VC394_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HEP5994830.1"
                     /translation="MSNWTTYPQKNLTQAEQLAQLIKEADALVVGIGAGMSAADGFTY
                     IGPRFETAFPDFIAKYQFLDMLQASLFDFEDWQEYWAFQSRFVALNYLDQPVGQSYLD
                     LKEILETKDYHIITTNADNAFWVAGYDPHNIFHIQGEYGLWQCSQHCHQQTYKDDTVI
                     RQMIAEQKNMKVPGQLIPHCPECEAPFEINKRNEEKGMVEDADFHAQKARYEAFLSEH
                     KEGKVLYLEIGVGHTTPQFIKHPFWKRVSENPNALFVTLNHKHYRIPLSIRRQSLELT
                     EHIAQLISATKTIYQKS"
     gene            7304..8323
                     /locus_tag="VC394_001368"
     CDS             7304..8323
                     /locus_tag="VC394_001368"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEP5994831.1"
                     /translation="MYLIEPIRNGKRITDGAVALAMQVYVQENLFLDDDILFPYYCDP
                     KVEIGKFQNAVVETNQEYLKEHHIPVVRRDTGGGAVYVDSGAVNICYLINDNGIFGDF
                     KRTYQPAIEALHHLGATEVEMSGRNDLVIDGKKVSGAAMTIANGRVYGGYSLLLDVDF
                     EAMEKALKPNRKKIESKGIRSVRSRVGNIREHLAPQYQGITIEEFKDLMVCQLLQIET
                     ISQAKRYDLTEKDWQQIDALTERKYHNWEWNYGNAPQYRYHRDGRFTGGTVDIHLDIK
                     KGYIAACRIYGDFFGKADIAELEGHLIGTRMEKEDVLATLNAIDLAPYLGAITAEELG
                     DLIFS"
     gene            8451..10121
                     /locus_tag="VC394_001369"
     CDS             8451..10121
                     /locus_tag="VC394_001369"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEP5994832.1"
                     /translation="MKSDIEIAQSVALQPITDIVKKVGIDGDDIELYGKYKAKLSFEK
                     MKAVEANEPGKLLLVTAINPTPAGEGKSTMSIGLADALNQMGKKTMLALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLQQGNDLGIDPRR
                     IIWKRVLDLNDRALRQVIVGLGSPVNGVPREDGFDITVASEIMAILCLATDLKDLKKR
                     LADIVVAYTYDRKPVYVRDLKVEGALTLILKDAIKPNLVQTIYGTPALIHGGPFANIA
                     HGCNSVLATSTALRLADYTVTEAGFGADLGAEKFLNIKVPNLPKAPDAIVIVATLRAL
                     KMHGGVAKSDLAAENCEAVRLGFANLKRHVENMRQFKVPVVVAINEFVADTEAEIATL
                     KALCEEIKVPVELASVWANGAEGGLALAKTVVRVIDQEAADYKRLYSDEDTLEEKVIN
                     IVTQIYGGKAVQFGPKAKTQLKQFTEFGWDKLPVCMAKTQYSFSDNPSLLGAPTDFDI
                     TIREFVPKTGAGFIVGLTGDVMTMPGLPKVPAAMAMDVAENGTALGLF"
     gene            10206..11783
                     /gene="cls"
                     /locus_tag="VC394_001370"
     CDS             10206..11783
                     /gene="cls"
                     /locus_tag="VC394_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEP5994833.1"
                     /translation="MIIKKKAKVKYLLHKGKHGFLRGIFSRTTIIVLLIILQLVFLFQ
                     SYAWMEQYRVWITILESVFAITIVLYLVNSDMDAISRMTWLILIMIAPLLGSLFLIYT
                     KLDWGYRGLKQRINHLVDLSAPYLSDDDAILEVLKDSTSTTYHLVQYLERSRGNFPIY
                     NNTRVTYFPTGETFFDSLKEQLFLAKKYIFLEFFIIAEGQMWGEILSILEKKVSEGVE
                     VRVLFDGMNELSTLSSDYAKRLEQIGIKAKSFLPISPFISTYYNYRDHRKIVVIDGEV
                     SFTGGINLADEYINEVERFGHWKDAGLMLEGEATDSFLILFLQMWSITEKELIIDPYL
                     SDHSLKLPSDGYVIPYGDSPLDTDKIGKNVYIDILNHAKEYVYIMTPYLILDSEMEHA
                     LRFASERGVDIRIIMPGVPDKGVPYALAKTYYKALMTSGVKIYEYQPGFVHSKVFISD
                     NTKAVVGTINLDYRSLYHHFECATYLYRVSVIADIVNDFNEAQKQSLLMTSDHLTQRP
                     WYQKLIGLLVRTIAPLL"
     gene            12123..12461
                     /locus_tag="VC394_001371"
     CDS             12123..12461
                     /locus_tag="VC394_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994834.1"
                     /translation="MQIIKRDGHVADFDPDKIYQAIIKAARTVYVIDDTWRQNLTHVT
                     KKVVIDLEEAHVERPTINMVQSLVENRLMDAGYITIAEHYISYRLQRDLERNGYGDKI
                     VVHLRFEQIK"
BASE COUNT         3931 a         2175 c         2628 g         3913 t
ORIGIN      
        1 aaaaaaacac taattatttg cagctgttta caaaactttt tccatatttt caagtagcta
       61 atgtaagaaa gttaaaataa ccaagttttt aaaagagaaa aactcggtta ttgtgaaagc
      121 taattacctt tttagtcttg atagtgttgg aataagtaca aaggtaataa tagctgagat
      181 gatcatctca acaatagcgt ttgatgagat gatggctgtt agtagtgctt taatatttcc
      241 atcaaaaact gtcgcgaaaa aaataaatat cccagataat acaaatatag tatttgttag
      301 agaaccaatg ataccggata ctattaaacc aaactgatta ggaatcaatt tatagcaata
      361 ataaggggta ataccaatca aaatacgtgg gataatagca attattaaag aagcgaaagt
      421 tccatgatcg acaaaaggtg aaaagagata atttgtaggc agtagaatga tggtatttgt
      481 aatgacactt ataatcccca ttaatcctcc taaaatagca cctatccttg ggccatataa
      541 gacagaagca ataataactg ggatatggac aagcgttggt ttgattggaa taggccaaat
      601 attaaagaca agactgctca caaaatgaat gactaacatg atggcaaaaa agatagcaat
      661 acgagaaata tctgctgatt tacggtgatt cattttactc tccaaaaaat ataatactta
      721 attctaaaaa ggtgatcaga aaaacgactg ttaaagtttc caaatcgtat caatagtagc
      781 taagataaca tcaatatcag ccaaagcacc aggtccttta tctccacagg ctaataagct
      841 agattttgga ggaatttctg taaaccctat tgtcgataag cgtttaatat tttcttgggt
      901 aatagggttt tgatacattt tggtattcat ggctggggct attaattttg gtgtggtagc
      961 tggtagggca agagcaacac ttgttacaat attatcagcg aatccatatg ctagatgagc
     1021 aatagtatta gcagaggcag gtgcaacaat aaataaatct gtgcgttttg ctaattcgat
     1081 atggttgata acttttgggt catgctcatc cataacatct aaatgaatag cattttttga
     1141 taacacttgc agggttaatg gtgtaataaa ttgtgtagca gcctgagtca ttatgatgtg
     1201 aacatcatag ccaattttag ttaattgact cgtaagatct gctgctttat aggcagatat
     1261 actaccagaa acagctaagg taatatgttt agtcatgttt ggtcatcctt tcatataata
     1321 agtcagcaat tgcctctttg gtatctgcag atgcaacttc gttattggat atcaaaagag
     1381 ccttgtgcat acctgtttgg atatcaacta aatcattggc gagaatataa tcagcatggt
     1441 ttttagctag acttgctctg gctactttaa ttaactcttc ttgaggcaca ttaactagaa
     1501 gtttaaagcc gactagttta atattaggat tccactgttt aacataagag ataactttgg
     1561 gtgttttttt caaaaaaaga acctgataat cagatgcaga tgagattttc ggctcactgt
     1621 tatgttcaca tagaaaacaa ttaaggttat cagcttgaga gacttgttcc aaatcagtca
     1681 tgtagactgg agtatagtca gaaacagcca tactatgaat tagaatgtca tggtgaggaa
     1741 cctgatcttt gagggcagcc attaagtcgt taactgtttc cacttcaata atccttagga
     1801 gcttatttgg caggggttta gtggctgttt tagtagtgac tagggtaacg tcatgatgat
     1861 attgtaaaaa tcgctctgtg attaattttc caagttgccc agttgagtga ttagtgatac
     1921 ctcgtactgc atctataggt tcagttgttc cacctgatgt tattattaat ttcatagtca
     1981 tattttatca aaaaaggagc tgatagtaaa cggaagataa tgacgccaga cattgtcttg
     2041 cgtttgtatt aatatggaat agtaatagat ttctagtaat taaaacgaag ttctggaaag
     2101 caagacacta tagtagctaa aatctaaatt ttaaggtaaa atagagcaag cggtttcaag
     2161 tgtgaaagga agaattgtga taactattag agatttagcg tcactgccga tttttgttta
     2221 cggtgatgga aataaaaaag ttccaggagc tttgagtcct tttgtgtgga cagaggtgtt
     2281 tttaaaaact atcaatcaag aacctaatca actgatctta catatttggc cgatgacaag
     2341 aacagttata ttagggatgt tagatagaca gcttccctac tttgagctgg ctaaaacaga
     2401 gattggcaat aatggttatg ttcctgttac tcgtaatatt ggcggtttgg ctgttgtcgc
     2461 tgatgatggg attttgaatt tttcgcttgt tattccagac catttttcgg aatctatcag
     2521 tattagtaat gcttatttaa taatggttga tgtcattaga gagagttttt cggattatta
     2581 tcagcgtatt gaatatcacg aaattaaaaa ttcttattgc cctggtaatt ttgatcttag
     2641 tattgcaggc cgcaagtttg caggtatcgc ccagcgtcgt atcaaaaagg gaattgttgt
     2701 gtctatttac ttgagcgttt gtggggatca agcagcgcgt ggtcaactca tcaaggattt
     2761 ttatgaggcc ggaacacaag gagaagttac caaggttaat taccctcaaa ttgatccaga
     2821 atgcatggca actctttcag aactccttga gacgcctttt acagtggcag aagtccttga
     2881 acgtcttcga ttgaccttgc gacagcttgg cttttccctc acggaaaaaa gtccagatca
     2941 agccttactg actaattttg atgctgtcta tgaacggatg cagctagaag tcgtcagaaa
     3001 ggaaggcaaa tgaaagaaag atttagtcct ttatttgagc ctttgacttt gccaaatggg
     3061 agtcaattgg ataatcgctt tgttttgtct cctatggtga ccaactcatc cacaaaagac
     3121 ggttatgtca cccaagatga tgtatcctat gctttgagac gagcagcttc tgcccctcta
     3181 caaattacag gagcggccta tgtggatcct tatggtcaat tgtttgaata tggttttagt
     3241 gttacaaaag atgccgatat ttctggcttg aaagagctag ctcaagcgat gaaggcaaag
     3301 ggagctaaag ctgtcttaca actgacccac gctggccggt ttgctagcca tgccttgact
     3361 aaatatggct ttgtctatgg gcctagttac atgcagttga gatctcccca acctcatgaa
     3421 gtcaaacctt taacaggtca gcaaatagag gaattgattg cagcttacgc tcaggccact
     3481 cggcgggcaa ttcaagcagg ctttgatggc gtggaagtat cttcggcgca gcgcttattg
     3541 attcaaacgt tcttttcgac tttttcgaac aagaggacag atagttatgg ttgtcaaaca
     3601 ctttttaatc gctctaagtt aactttagct gttttgcaag ctgttcaaca ggtgattaat
     3661 caagaagctc ctgatgggtt tatttttgga tttcgtgcta caccagaaga aactagggga
     3721 aatgatattg gttatagtat tgacgagttt ttgcagttga tggattgggt acttaatatt
     3781 gcaaaacttg attatttagc gatagcaagt tggggccgtc atgtatttcg aaatactgtt
     3841 cgttctcctg gcccttatta tggaaggcga gttaatcagg tagttagaga ttatttaaga
     3901 aataagctac ctgttatggc tacaggggga atgaacacac ctgacaaagc tattgaagca
     3961 ctagctcatg ctgattttat tggagtatct acaccttttg tcgtggatcc agaatttgct
     4021 cataaaatca aagaagggtg tgaagagagt attcatttaa ggattcgacc tgctgatttg
     4081 aaaagtttag cgattcctca ggcttctttc aaagatattg tacctttgat ggactacggg
     4141 gaatcattgc ctaaagaatc aagaacttta tttcgtagtc ttactcacaa ttataaggag
     4201 attaaatgaa agtcagtatt cttgattatg gggtgattga taaagaaaaa acaccccaag
     4261 aagctttatt agaaacgcgc tgcttagcac aagtagccga caaacttggt tttcatcgtt
     4321 tttgggtagc agagcatcat aatatttatg cctttgcaat aagcagtcca gagctgttga
     4381 tgatgcattt agcagatcat accaaacaga ttcggatagg ttcaggaggt attatgcctc
     4441 tacattatag tagctttaag attgcagaat ggataatgac tttagaggcc ttgcacccta
     4501 atcggattga tttaggtatt ggcaactctt taggaactac gttagttcaa agagctctat
     4561 caagtatcca ttgcaaagac agctatagtc aggttgtcac agaattatac cagtatctta
     4621 atcctgatca tttgtcacca ctaccgattt ttgttaaccc taggggtaat acttaccctc
     4681 agatttggac gctaagcaat agtttggaga cagcagaact ggctggtcaa ttaggtttag
     4741 gctatacctt tggtattttc ccctatatac caaaagatcc tataacagaa gctaaaagag
     4801 tatcggcaca ttatagaaag gcttttaggc cttcaaagct attaaaaatc cccaagttaa
     4861 tattagcagt ttttattgtc ctttctgata ccgacgagga agcagaagct ttggctaaac
     4921 cgttggacat ttggatgctt ggtcagcaag attttaatga atttaagacc taccctgatg
     4981 ttgaggaggc tagaaactat cacttaacag agaaacaaag agaagccatt gcagcaaatc
     5041 gtagtcgcat ggttattgga tcacctcaca cagtcaagaa acaattggat aggctgatag
     5101 aagcttgtca agctgacgaa ttattagcca tccccttagt tcctgaattt gccaatagac
     5161 aacgaacctt agagctgcta gcagacttat acgtgaccat ttaaccttac caaaaacaat
     5221 tccctaaact gaaaggaaag aaacaatgaa aaaaattgca aattacttat taattgagaa
     5281 aacagatgac cgctatacca ttagcatgac accagaatta caagacgata ttggtactat
     5341 tggttacgct gaatttacgg ataacgacca ccttgccgtt gatgacatta tcttaaatct
     5401 agaggcttct aagaccgtca tgtctgtctt gtcaccattg gctggggcag ttgttgagcg
     5461 caatgaggca gccacactaa caccaaccct tttgaattca gaaaaagctg aggaaaattg
     5521 gattgttgtg ctaacagatg ttgatcaagc agcttttgat gccttagaag atgcctagtt
     5581 catttgattt attaggggaa atgattgatc tgttgcaaac agagcagctt actagttctt
     5641 gggcctgtcc cttgcctaat gctttaacaa agcgacaaga cttgtggcgg gcgctcatta
     5701 accagcgccc tgctttgcct ttatcaaaag attacttgaa tctggaagat gcttatctag
     5761 atgattggcg tgcttctttt gttcctgtat cagtcaagga ctgccaaaaa actaattata
     5821 ccagtctttt tctttaccat ggggatatta ggtaccttgc tgtggatgct attgtcaatg
     5881 ctgctaatag tgagttattg ggatgtttta tccctaacca tggctgtatt gataatgcga
     5941 ttcatacgtt tgccggaagt cgtttacgct tagcttgtca ggcaatcatg actgaacaag
     6001 gccgtaagga agccattggc caagcaaagc ttaccagtgc ttatcatcta ccagcctctt
     6061 atattattca taccgtaggg ccaagaatta ccaaaggacg acacgtttct ccgattcgag
     6121 ctgatttgtt agcacgttgt tatcgttcta gtcttgattt ggcggttaaa gcaggattga
     6181 ctagcctagc tttttgctct atttcaacag gagaatttgg ctttcctaaa aaagaagccg
     6241 ctcagattgc cattaagaca gttttaaaat ggcaagcaga acatccagaa tctaagactt
     6301 taactatcat ttttaatacc tttacatcag aagacaaggc cttatacgac acttatttgc
     6361 aaaaggagaa taactgtgag taattggaca acctatcctc agaaaaatct cacccaggca
     6421 gaacaactgg cccaattaat caaagaagca gatgcacttg tagttggtat tggagctggc
     6481 atgtctgcag ctgatggttt tacttatatc gggccgcgtt ttgagacggc attcccagat
     6541 tttattgcta agtatcagtt tttggatatg ctgcaagcta gcctttttga ttttgaagat
     6601 tggcaagaat attgggcttt tcaaagtcgc tttgtcgctt tgaattatct tgatcagccc
     6661 gtaggccagt cttatcttga tttaaaagag attttggaaa ccaaagacta tcatattatt
     6721 actactaatg ctgataatgc tttttgggtg gctggttatg atcctcataa catttttcac
     6781 attcaaggag aatatggttt gtggcaatgt agccagcact gccatcagca aacttataag
     6841 gacgatacgg tcatccgtca aatgatcgca gagcaaaaaa acatgaaagt cccaggtcaa
     6901 ctgattcctc attgccctga atgtgaggcg ccttttgaaa tcaataagcg taatgaagaa
     6961 aaaggaatgg ttgaagatgc tgactttcat gcccaaaaag cacgttatga ggctttttta
     7021 tcagaacata aagagggtaa agtgctttat ctagaaattg gtgtgggaca tacgacgcct
     7081 cagtttatca aacacccctt ttggaaacgt gtctcagaaa accctaatgc cttattcgtt
     7141 accttgaatc acaaacatta ccgcatccct ctgtcaataa ggcgacaaag cctagaactg
     7201 acagaacaca ttgcacaatt gattagcgcc acaaaaacca tttaccaaaa atcgtaacca
     7261 aagccaatag acggttaatt aagagatata gaaggagact aacatgtacc ttattgaacc
     7321 gattcgtaac ggtaaacgta tcaccgatgg agctgttgct ctagccatgc aagtttatgt
     7381 tcaagaaaat ctatttttag atgatgatat tttatttccc tactattgtg atcccaaagt
     7441 tgaaattgga aaattccaga atgcggttgt agaaactaat caagagtatt taaaagagca
     7501 tcatatccct gttgttcgcc gagatactgg cggtggtgcc gtttatgtag attctggtgc
     7561 tgttaatatt tgctacttga ttaatgataa tggtattttt ggtgatttta aaagaaccta
     7621 tcagcccgct attgaagccc ttcatcattt gggagcaaca gaggttgaaa tgtctggacg
     7681 gaatgacttg gttattgatg ggaaaaaggt atctggcgct gcgatgacta ttgctaatgg
     7741 ccgtgtttat ggggggtatt cccttttgct agatgtggac tttgaagcca tggaaaaagc
     7801 attaaaacct aatcgcaaaa aaattgaatc aaaaggcatc aggtcagttc gtagtcgcgt
     7861 tggaaacatc agagaacatt tagcaccaca ataccaaggt attacgattg aagagttcaa
     7921 agatttaatg gtttgccaat tgttacagat tgagacgatt agccaagcta agcgttatga
     7981 tttaacggaa aaagactggc aacaaattga tgctttaaca gagcgtaaat atcacaattg
     8041 ggaatggaat tatggcaacg cgcctcaata ccgttaccat agagatggcc gttttaccgg
     8101 tggaacagta gatattcatt tagatattaa aaaaggctat attgcagctt gtcgcattta
     8161 cggagatttt tttggcaagg cagacattgc agaacttgaa ggtcacttga taggaacacg
     8221 aatggaaaaa gaggatgtct tagctaccct caatgcaatt gatttagccc cttatttagg
     8281 tgcgattaca gcagaagaat taggagattt gatattttct tgataaaaaa gatacctcag
     8341 accggtaaag tgcgattaaa aacgaacaat aattgtttta attcaaataa atgtatgtat
     8401 gatatgaatt gtgatataat gacgtcatta cgtaaaattg aggagtcatc atgaaatcag
     8461 atatcgaaat tgcccaaagt gtcgccctac aaccaatcac tgacattgtt aaaaaggttg
     8521 gaatagacgg cgatgacatt gagctttatg gcaaatataa ggctaaattg tcttttgaga
     8581 aaatgaaagc tgtcgaagct aatgagcctg gaaaattact cttggtaact gctattaacc
     8641 ctactccagc gggagaagga aaatcaacca tgtctattgg actggcagat gccttaaatc
     8701 aaatgggaaa aaaaaccatg cttgccttac gtgaaccctc ccttggtcct gttatgggga
     8761 ttaaaggcgg ggcagcaggt ggtggttatg cccaagtatt gcctatggaa gacattaatc
     8821 ttcactttac tggtgacatg catgccatta caaccgctaa taatgcatta tctgctttga
     8881 ttgacaatca tttgcagcaa ggaaacgatt taggaattga tcctcgccgc attatttgga
     8941 agcgcgtgtt ggaccttaat gatcgtgctc ttcgtcaggt aattgttggc ttaggcagtc
     9001 cagtgaatgg tgtgccacgc gaagatgggt ttgatatcac agtagcttct gaaatcatgg
     9061 caattttatg tttggcaact gacttgaaag atctaaagaa acgtcttgct gatatcgtcg
     9121 tggcttatac ctatgatcgt aagcctgtct atgtgcgtga cttgaaggtt gaaggagcat
     9181 tgacattaat tctaaaagat gctatcaagc caaacttggt gcagactatt tatggaacac
     9241 cagctcttat tcatgggggg ccatttgcta atattgctca tggttgtaat tctgtccttg
     9301 ctacctcaac agctttgcga ttagcagatt acactgttac cgaagcagga ttcggggccg
     9361 acctaggagc tgaaaaattc ctaaatatca aagtacctaa tcttcctaaa gcaccagatg
     9421 ctatcgtcat tgttgcaacc ttgcgcgccc ttaaaatgca tggaggtgtc gccaaatcag
     9481 atttagccgc tgaaaattgt gaggctgttc gcttaggatt tgccaactta aaacgtcacg
     9541 tggaaaacat gcgccaattc aaagttccag ttgtggtggc tatcaatgaa tttgtagcag
     9601 ataccgaggc tgaaattgct accttaaaag cgctttgtga agagattaag gttccagtag
     9661 aattagccag tgtttgggca aatggagctg agggaggctt agcattagct aaaacagtgg
     9721 ttcgtgtgat tgatcaagaa gctgctgact acaaacgtct ctattcagat gaggatactt
     9781 tggaagaaaa agtgatcaat atcgttaccc agatttatgg tggcaaagct gttcaatttg
     9841 gccccaaggc caaaacacaa ttgaagcagt tcacagaatt cggttgggat aaattgcctg
     9901 tttgtatggc taagacccaa tacagttttt cagataaccc atccttgctt ggagcgccaa
     9961 ccgattttga tattaccatt cgtgaatttg ttcctaaaac aggggcaggt tttattgttg
    10021 gtttaacagg tgatgtgatg accatgcctg gtttgcctaa agttccagct gccatggcaa
    10081 tggacgtagc agaaaatggc acagccctcg gtctctttta ggaaaaattg ctcgaataat
    10141 tcgagcaagc gctcctttct tcccgcttta ctgtgatata ataaggtaac tgagattttg
    10201 gagggatgat tatcaaaaag aaagcaaaag taaaatattt gctccataaa gggaaacatg
    10261 gatttttaag aggaattttt agtagaacaa caatcattgt attattaatt attttgcaat
    10321 tagttttctt atttcaatct tatgcctgga tggagcagta ccgtgtttgg ataacgattc
    10381 ttgaaagtgt ctttgctatt actattgttt tatatttagt taatagtgat atggatgcta
    10441 tttcaagaat gacgtggtta attttgatta tgattgctcc attactaggg tcactgtttt
    10501 taatttatac aaaattggat tggggttaca gagggctaaa acaaagaata aatcatcttg
    10561 tagatctgtc tgcaccttac cttagcgatg atgacgctat attagaggta ttaaaggata
    10621 gcacatcaac cacatatcat ttagtacagt acttagaaag aagtcgtggc aactttccga
    10681 tttataataa tacaagggta acttacttcc caacaggtga aacttttttt gatagtttaa
    10741 aagaacagtt attcttagct aaaaagtaca tttttcttga attttttatt attgcagaag
    10801 gccaaatgtg gggagaaatc cttagtattc tagaaaaaaa agtcagtgaa ggtgtagaag
    10861 ttagggtgtt gtttgatggc atgaacgaac tatcaacgct atcatcagat tacgccaaga
    10921 gattagaaca aatagggatt aaagctaaat catttttacc gatttcaccc tttatctcta
    10981 cctattacaa ttatcgagat caccgaaaaa ttgtcgttat tgatggggag gtatcattca
    11041 ctggaggtat taatctagca gatgagtaca ttaatgaagt agagcgtttt ggccactgga
    11101 aagatgctgg tttaatgctt gagggtgaag caacagacag ctttttaatt ttgtttttac
    11161 aaatgtggtc tatcacagaa aaagaactga ttattgatcc ttatctttca gatcattctt
    11221 taaaacttcc ttcagatggc tatgttattc cctacggtga ttccccgctt gatactgata
    11281 aaataggtaa aaatgtttat atagacattt taaatcatgc taaagagtac gtttatatca
    11341 tgacacctta ccttatttta gatagcgaga tggaacacgc tttacgattt gcatcagaac
    11401 gtggagtaga tatccgaatt attatgccag gagtgccaga taagggggta ccatatgcct
    11461 tagctaaaac ctattataaa gctttgatga cttctggagt aaaaatttac gagtatcaac
    11521 cagggttcgt tcattcaaag gtatttatct ctgataatac aaaagcagtt gtcggaacaa
    11581 ttaacttaga ctatcgtagc ctttatcatc attttgaatg cgcgacttat ttatatcgtg
    11641 tgtcagttat tgctgatatt gtgaatgact ttaatgaagc tcaaaagcaa tcactattga
    11701 tgacatcaga tcatttgacg caacgtcctt ggtatcaaaa attgatagga ttattagtac
    11761 gaacaattgc ccctctcttg taattttcgt aaaatatgtt ataatatctg tatgcgatga
    11821 gtcgattgtg actgcaaagt cacttttgcg caggggaggt cattaaggcg ggaagccgac
    11881 tttgataatc tgtgtgaacc attttatcac aatcttattt attaagaatg ccccttggat
    11941 tgagatattc tcaatccttt ttttaatttt attatcttag ttaaaattca gaatgtatca
    12001 gtgattactg tgtttcaaaa taagaaagta gtttttcggg tatgagtgct atgacttgac
    12061 aaatgctaat aacatttgca taatgtaagt agagatgaaa acttatgaaa agagatgatc
    12121 ttatgcaaat tattaagcgt gatggccacg ttgctgattt tgatccagat aaaatttatc
    12181 aagcaattat taaagcagca aggacggtct atgttattga tgacacgtgg cgacaaaact
    12241 taacacatgt cactaaaaaa gtagttattg atttagaaga agcacatgta gaacgtccta
    12301 ctatcaatat ggtccaatcc cttgtggaga atcgtttgat ggatgctgga tatattacca
    12361 ttgcagaaca ttacatttct tatcgtcttc agcgagattt agagcgcaat ggctatggtg
    12421 ataagattgt ggttcacttg cgttttgaac aaataaaata aaaacgcttc taatggcatt
    12481 ttagtgtctt tgaggggata aggtttaaat acctaacaga taaaaatctg ttaggtattt
    12541 ttttgctact attaggtgta ttctatatca catagaactt aagcattcaa gctttaattt
    12601 aactttctgt ttattttttg ctacattttg ggaaactccc ctccttt
//
LOCUS       DATXQK010000043        12169 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.043, whole genome shotgun sequence.
ACCESSION   DATXQK010000043 DATXQK010000000
VERSION     DATXQK010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12169
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.043"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            52..423
                     /locus_tag="VC394_001372"
     CDS             52..423
                     /locus_tag="VC394_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEP5994835.1"
                     /translation="MKEKELRRSMAVFPIGTVMTLTDLSARQIRYYEDQGLIKPERTQ
                     GNRRMFSLNDMDRLLEIKDFLSEGLNIAAIKREYVERQGKLMQKQKALTDADVRRILH
                     DEMLTQSGFSTPSQHIGNFRI"
     gene            461..1807
                     /gene="glnA"
                     /locus_tag="VC394_001373"
     CDS             461..1807
                     /gene="glnA"
                     /locus_tag="VC394_001373"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEP5994836.1"
                     /translation="MAITVADIRHEVKEKNVTFLRLMFTDIMGVMKNVEIPATKEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWIVFPWGDENGAVAGLICDIYTAEG
                     KPFAGDPRGNLKRALKHMNEIGYKSFNLGPEPEFFLFKMDDKGNPTLEVNDNGGYFDL
                     APIDLADNTRREIVNILTKMGFEVEASHHEVAVGQHEIDFKYADVLKACDNIQIFKLV
                     VKTIAREHGLYATFMAKPKFGIAGSGMHCNMSLFDNQGNNAFYDETDKRGMQLSEDAY
                     YFLGGLMKHAYNYTAITNPTVNSYKRLVPGYEAPVYVAWAGSNRSPLIRVPASRGMGT
                     RLELRSVDPTANPYLALAVLLEAGLDGIINKIEAPEPIEANIYTMTMEERNEAGIIDL
                     PSTLHNALKALQKDDVVQKALGYHIYTNFLEAKRIEWSSYATFISQWEIDHYIHNY"
     gene            complement(2035..3717)
                     /locus_tag="VC394_001374"
     CDS             complement(2035..3717)
                     /locus_tag="VC394_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEP5994837.1"
                     /translation="MTNISLKPNEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKF
                     PEDDLLGIDYVIPDYSYIVDNLDRVKALVITHGHEDHIGGIPFLLKQANIPIYAGPLA
                     LALIRGKLEEHGLWREATVYEINHNTELTFKNMSVTFFKTTHSIPEPVGIVIHTPQGK
                     IICTGDFKFDFTPVGDPADLQRMAALGEEGVLCLLSDSTNAEIPTFTNSEKVVGQSIL
                     KIIEGIHGRIIFASFASNIYRLQQAAEAAVKTGRKIAVFGRSMEKAIVNGIELGYIKV
                     PNGTFIEPSELKNLHASEVLIMCTGSQGESMAALARIANGTHRQVTLQPGDTVIFSSS
                     PIPGNTTSVNKLINTIQEAGVDVIHGKVNNIHTSGHGGQQEQKLMLSLIKPKYFMPVH
                     GEYRMQKVHAGLAMDIGIPKENIFIMENGDVLALTSDSARIAGHFNAQDIYVDGNGIG
                     DIGAAVLRDRRDLSEDGVVLAVATVDFNTQMILAGPDILSRGFIYMRESGDLIRESQR
                     VLFNAIRIALKNKDASIQSVNGAIVNALRPFLYEKTEREPIIIPMVLTPDKH"
     gene            complement(3719..3949)
                     /locus_tag="VC394_001375"
     CDS             complement(3719..3949)
                     /locus_tag="VC394_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEP5994838.1"
                     /translation="MIYKVFYQETKDQSPRRESTKALYLNIDATDELDGRIKARRLVE
                     DNTYYNVEFIELLSDKHLDYEKETGVFELTEF"
     gene            4233..4931
                     /gene="tsaB"
                     /locus_tag="VC394_001376"
     CDS             4233..4931
                     /gene="tsaB"
                     /locus_tag="VC394_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEP5994839.1"
                     /translation="MKTLAFDTSNKTLSLAILDDETLLADMTLNIQKNHSVSLMPAID
                     FLMTCTDLKPQDLERIVVAKGPGSYTGLRVAVATAKTLAYSLNIALVGISSLYALAAS
                     TCKQYPNTLVVPLIDARRQNAYVGYYRQGKSVMPQAHAPLEVIIEQLVEEGQLIFVGE
                     TAPFAEQIQKKLPQAILLPTLPSAYECGLLGQSLAPENVDAFVPQYLKRVEAEENWLK
                     DNEIKDDSHYVKRI"
     gene            4903..5358
                     /gene="rimI"
                     /locus_tag="VC394_001377"
     CDS             4903..5358
                     /gene="rimI"
                     /locus_tag="VC394_001377"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEP5994840.1"
                     /translation="MIVTTLSESNMKTVEEQAKNIYQLLEMVYGTSPWTLEQVLIDIR
                     RDQTDYFLLYDHDKLLGFLAIQDLAGEVEMTQIAILPSHQGLGLASQLMTHLDSIESD
                     IFLEVRESNHRAQGLYQKFGFKFIGKRPDYYRNPIETALLMKREGKHDR"
     gene            5348..6376
                     /gene="tsaD"
                     /locus_tag="VC394_001378"
     CDS             5348..6376
                     /gene="tsaD"
                     /locus_tag="VC394_001378"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEP5994841.1"
                     /translation="MTDRYILAVESSCDETSVAILKNESTLLSNVIASQVESHKRFGG
                     VVPEVASRHHVEVITTCFEDALQEAGISASDLSAVAVTYGPGLVGALLVGLAAAKAFA
                     WANHLPLIPVNHMAGHLMAAREQKPLVYPLIALLVSGGHTELVYVPEPGDYHIIGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDQLAHKGQDTYHFPRAMITEDHLEFSFSGLKSA
                     FINLHHNAKQKGNELILEDLCASFQAAVLDILLAKTKKALSRYPAKMLVVAGGVAANQ
                     GLRDRLAQEITHIEVVIPKLRLCGDNAGMIALAAAIEYDKQHFANMSLNAKPSLAFDQ
                     FPDSFVIN"
     gene            complement(6547..6816)
                     /gene="rpsN"
                     /locus_tag="VC394_001379"
     CDS             complement(6547..6816)
                     /gene="rpsN"
                     /locus_tag="VC394_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEP5994842.1"
                     /translation="MAKKSKIAKYQKQLQLIEQYADLRRDLKAKGDYESLRKLPRDSN
                     PNRLKNRDKIDGRPHAYMRKFGVSRINFRDLAHKGQLPGVTKASW"
     gene            complement(7037..7795)
                     /locus_tag="VC394_001380"
     CDS             complement(7037..7795)
                     /locus_tag="VC394_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEP5994843.1"
                     /translation="MSTNDLTKKLKKLKHVQVYNTIFQLIQDGTYSPGMQLPSEPELA
                     RQLNVSRMTLRKSLALLQEDHLIKNIRGKGNFILKTPETKYHQGFEYLQHPIYASLSS
                     DITKVELEYRIEVPTVAITASLKQETPVVIIVDRWYHSQNKAIAYSLSFIPIEVISKY
                     AINLNQEEPLLTFLEEKIYESGKASHSCNQIGYTKTGNYTATKYTLSENSAFILIQET
                     LYNGKDILVSTKHYVPADLFDLKVQSQSCQSDTH"
     gene            8035..8814
                     /gene="udp"
                     /locus_tag="VC394_001381"
     CDS             8035..8814
                     /gene="udp"
                     /locus_tag="VC394_001381"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HEP5994844.1"
                     /translation="MQNYSGEVGLQYHLQIRPGDVGRYVIMPGDPKRCAKIAEHFDNA
                     VLVADSREYVTYTGTLNGEKVSVTSTGIGGPSASIAMEELKLCGADTFIRVGTCGGID
                     LDVKGGDIVIATGAIRMEGTSKEYAPIEFPAVADLEVTNALVNAAKKLGYTSHAGVVQ
                     CKDAFYGQHEPERMPVSYELLNKWEAWKRLGTKASEMESAALFVAASHLGVRCGSDFL
                     VVGNQERNALGMDNPMAHDTEAAIQVAVEALRTLIENDKSQ"
     gene            8835..10037
                     /locus_tag="VC394_001382"
     CDS             8835..10037
                     /locus_tag="VC394_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEP5994845.1"
                     /translation="MQFIYSIIGILLVLGIVYAISFNRKSVSLSLIGKALIVQFIIAL
                     ILVRIPLGQQIVSVVSTGVTSVINCGQAGLNFVFGSLADSGAKTGFIFAIQTLGNIVF
                     LSALVSLLYYVGILGFVVKWIGKGVGKIMKSSEVESFVAVANMFLGQTDSPILVSKYL
                     GRMTDSEIMVVLVSGMGSMSVSILGGYIALGIPMEYLLIASTMVPIGSILIAKILLPQ
                     TEPVQKIDDIKMDNKGNNANVIDAIAEGASTGAQMAFSIGASLIAFVGLVSLINMMLS
                     GLGIRLEQIFSYVFAPFGFLMGFDHKNILLEGNLLGSKLILNEFVSFQQLGHLIKSLD
                     YRTALVATISLCGFANLSSLGICVSGIAVLCPEKRSTLARLVFRAMIGGIAVSMLSAF
                     IVGIVTLF"
     gene            10067..10738
                     /gene="deoC"
                     /locus_tag="VC394_001383"
     CDS             10067..10738
                     /gene="deoC"
                     /locus_tag="VC394_001383"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEP5994846.1"
                     /translation="MEVKDILKTVDHTLLATTATWPEIQTILDDAMAYETASACIPAS
                     YVKKAAEYVSGKLAICTVIGFPNGYSTTAAKVFECQDAIQNGADEIDMVINLTDVKNG
                     DFDTVEEEIRQIKAACQDHILKVIVETCQLTKEELIELCGVVTRSGADFIKTSTGFST
                     AGATFEDVEVMAKYVGEGVKIKAAGGISSLEDAKTFIALGASRLGTSRIIKIVKNEAT
                     KTDSY"
     gene            complement(10833..12002)
                     /locus_tag="VC394_001384"
     CDS             complement(10833..12002)
                     /locus_tag="VC394_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEP5994847.1"
                     /translation="MTQYDTIIIGGGPAGMMAAISSSYYGYKTLLIEKNRRLGKKLAG
                     TGGGRCNVTNSGNLDDLMAGIPGNGRFLYSVFSQFDNHDIIAFFEENGVKLKEEDHGR
                     MFPTTDKSRTIIDALEKKIKALGGQVLTSTEVVSVKKQDDLFYLKSTDQTFTCQKLIV
                     TTGGKSYPSTGSTGFGHDIARHFKLTVTDLEAAESPLLTDFPHKVLQGISLDDVTLSY
                     DKHVITHDLLFTHFGLSGPAALRLSSFVKGGEIAELDFLPHLSTDDLTAYLSDQRDKN
                     IKNALKGLLPERVADFLSEDYPEKVKQLSPKQEKELLDKLKHLQIPITGKMSLAKSFV
                     TKGGVDLKEINPKTLESKKVPGLYFAGEVLDINAHTGGFNITSALCSGWIAGKSS"
BASE COUNT         3705 a         2084 c         2542 g         3838 t
ORIGIN      
        1 tgacaggtta tttttttcag cctataataa ggaagatgaa aggagtttgt catgaaagaa
       61 aaagaactta gacgctcaat ggctgttttt cctattggaa cggtgatgac attaacagat
      121 ctctctgcga gacaaattcg ctattatgaa gatcaaggtt taattaagcc tgaacgaacg
      181 caagggaatc ggcgtatgtt ttccttaaat gacatggacc gacttttgga aattaaagat
      241 tttttgtctg agggattaaa tattgcagct attaaacgcg aatatgtcga gcgtcaaggt
      301 aagttaatgc aaaaacaaaa agctctaaca gatgcagatg tcagacggat cttacatgat
      361 gaaatgctga cacaaagcgg tttttctact ccctcacagc atattggtaa ttttcgtatt
      421 taagatatga attgtataaa atttaaagga gacctattta atggcaatca cagtagctga
      481 cattcgtcat gaagtcaaag aaaaaaatgt aacgtttctt cgcttgatgt tcactgatat
      541 catgggcgtt atgaaaaatg tggagattcc tgcaacgaaa gaacagttag acaaagtatt
      601 gtctaacaag gttatgtttg atggttcatc tatcgaaggt tttgtacgga tcaatgagtc
      661 agatatgtac ctttaccccg atttagacac ttggattgtt tttccctggg gagatgaaaa
      721 tggagcagtt gcaggtttaa tttgtgatat ttatacagca gaaggaaagc cttttgcagg
      781 agatcctaga ggaaatttaa aaagagccct gaaacacatg aacgagatcg gctacaaatc
      841 atttaatctt gggccagaac cagaattttt cctttttaag atggatgata aaggtaatcc
      901 gacacttgaa gttaacgata atggtggtta ttttgattta gcgccaattg acttagcaga
      961 caacacgcgc cgtgaaattg tgaatatttt aacgaaaatg ggttttgaag tggaagctag
     1021 tcatcatgaa gtggctgttg gtcaacatga gattgatttt aaatatgcag atgttttgaa
     1081 agcttgtgat aatattcaaa tttttaagct agttgtaaaa acgattgccc gtgaacatgg
     1141 actttatgct acttttatgg ctaaaccaaa atttggaata gctggatcag ggatgcactg
     1201 taacatgtct ttgtttgata accaaggtaa taatgctttt tatgatgaaa ctgataagcg
     1261 aggaatgcag ctatcagaag atgcttatta tttcttggga ggactaatga agcatgctta
     1321 taactacact gctatcacta accctacagt gaattcttac aaacgattag ttccgggtta
     1381 tgaggcacct gtttatgtcg cttgggctgg aagtaatcgt tcaccgctta tccgtgttcc
     1441 agcatcacgt ggtatgggaa cgcgtttgga gttacgttcg gttgatccga cagctaatcc
     1501 ttatttagcc ttggctgttc tcttggaagc tggattagat ggtatcatta acaaaattga
     1561 agctccagaa cccattgaag ctaacattta taccatgaca atggaagaac gaaatgaagc
     1621 aggcattatt gatttgccat caacgcttca taatgcctta aaagctcttc aaaaagatga
     1681 tgtggtacaa aaggcactag gttaccatat ctacactaat ttcttagaag caaaacgaat
     1741 tgaatggtct tcctatgcaa cttttatttc tcaatgggaa attgaccatt atattcataa
     1801 ttattagact tatcaggacc tgctcaagtt gagtgggtct tttggatgag ctattgtatt
     1861 tgagctagca agctattatt agctgatcgg acaccaattg attttccatt atagaaaact
     1921 aaaaaccagt ccatgttcta tgaggactgg tttttagttt gataattatc tgaactagaa
     1981 ctgatatgtt aaagatatag acagatagta ttaagaaatg agtcttattt gattttagtg
     2041 cttatcaggt gttaagacca tcggaattat aattggttca cgctcagtct tttcatacaa
     2101 gaatggacga agagcattga cgatagcacc gttaactgat tggatgctag catccttatt
     2161 ttttaaagca atacggatag cgttaaagag cacacgttgg ctttcgcgga tcaagtcacc
     2221 tgactcacgc atataaatga aaccacggct cagaatatct ggtccagcta aaatcatttg
     2281 tgtgttaaaa tcaacagttg caacagctaa gacaacacca tcttctgaca aatcacggcg
     2341 gtctcgtaaa acggctgctc caatatcccc gatgccatta ccatccacgt agatgtcttg
     2401 ggcattaaag tgaccagcaa tacgcgcact gtcactggtt aaagctagaa cgtctccatt
     2461 ttccataata aagatatttt cttttggaat accaatatcc atcgccagtc ctgcatgaac
     2521 tttttgcatg cgatactcac catgtactgg catgaaatat tttggtttaa tcaaactaag
     2581 catgagcttt tgttcttgct gtcctccatg accagaagta tgaatattat tgacttttcc
     2641 atgaataaca tcaacaccag cttcttggat ggtattgatt aatttattga cacttgtggt
     2701 attaccaggg attggacttg atgagaaaat cacagtatca ccaggctgta aagttacttg
     2761 acgatgagtg ccattagcga tacgagcaag tgctgccata gactcacctt gactacctgt
     2821 acacataatt aaaacttcac tagcatgaag atttttaagt tcgcttggct caatgaaggt
     2881 accatttgga actttaatat agccaagttc aataccgtta acaattgctt tttccataga
     2941 acgaccaaaa acggcaattt tacgtcccgt tttaacagca gcttctgcag cctgttgcaa
     3001 acggtagatg tttgaagcga aggaagcaaa aataatacga ccatgaatac cttcgataat
     3061 ttttaaaatg gattggccaa caactttttc agagttagta aaggttggga tttcagcatt
     3121 agttgagtct gataataagc aaaggacacc ttcttcacca agcgctgcca tacgttgtaa
     3181 atctgcagga tctcctactg gcgtgaagtc aaatttaaaa tcaccagtac agataatctt
     3241 accttgaggg gtgtggataa caataccgac cggttctgga atggaatgag tagtcttgaa
     3301 gaaagtgacg ctcatgtttt taaaagttaa ctcggtgttg tgattgattt cgtaaacagt
     3361 tgcttcacgc caaagaccat gttcttccaa tttaccacgg ataagagcaa gtgctaaagg
     3421 tcctgcgtag atagggatat tagcctgttt gagaagaaat ggaataccac caatgtggtc
     3481 ttcgtgacca tgtgtaataa caagagcttt tacccggtct aagttatcga caatgtaaga
     3541 atagtctgga ataacgtaat caattcccaa aagatcatct tctgggaatt tgataccggc
     3601 atcaacgata atgatctcgt cttgatactc aataccataa gtattcttcc caatctctcc
     3661 cagaccacca atggcaaaca ccccaacttc attaggtttt aaactgatat ttgtcatctt
     3721 agaactccgt taattcaaaa acaccggtct ctttttcgta atcgaggtgt ttgtcagaaa
     3781 gtagctcgat aaattccacg ttataatagg tgttatcttc aacgagacga cgagccttga
     3841 tacggccatc gagttcatca gtagcatcaa tattaagata gagtgcttta gtactttcac
     3901 gacgtgggct ttgatccttt gtttcttgat agaaaacttt ataaatcatt tgttttttat
     3961 tcctttcgtt atgaatcaat tatccaaaca ataataaata ggtcaaaagg taggtgttcc
     4021 attttgacgg ttttatatgt tgtcattatt gtgtagtaga ttaattcctg attctgtgca
     4081 attattataa ttataccata aacttagctt ttagtaaaaa atttaggtac atttatggta
     4141 tagtctaaat caaattaaga gtcagccttt ttgtttatga ttcttaattt gtgttataat
     4201 tgatactaga acttgcagta gaaaggcata aaatgaagac acttgcattt gatacctcaa
     4261 ataaaacctt gtcccttgct atacttgatg atgagacact tctagcagat atgaccctta
     4321 acattcagaa aaatcatagt gttagcctta tgcctgctat tgattttttg atgacttgta
     4381 ctgatcttaa acctcaagat ttagaaagaa tagtggttgc aaaaggccct ggatcttaca
     4441 caggtttacg agtggcagtt gctactgcaa aaacgttagc gtacagttta aatattgcat
     4501 tggtcgggat ttccagtcta tatgctttgg ctgcgtctac ttgtaaacag tatccaaata
     4561 ctttggtggt gccattgatt gatgctagaa ggcaaaatgc gtatgtaggt tattatcggc
     4621 aaggaaaatc agtgatgcca caagcccatg ctccactaga agttattata gaacaattag
     4681 tagaagaagg gcagctgatt tttgttgggg agactgctcc ttttgctgag caaattcaaa
     4741 agaaactacc tcaggcgata ctacttccaa ccctgccttc tgcttacgaa tgtggtcttt
     4801 tggggcaaag tttggcacca gaaaatgtag acgcctttgt ccctcaatat ctcaagagag
     4861 tggaagctga agaaaactgg ctcaaagata atgagataaa agatgatagt cactacgtta
     4921 agcgaatcta atatgaaaac agttgaagaa caagcaaaaa atatttatca gcttttggaa
     4981 atggtttatg gcacgtctcc ttggacttta gaacaagtat taatcgatat acggcgggat
     5041 caaacagatt atttcctact atatgatcat gataaattgc taggtttttt agctattcaa
     5101 gatttagctg gtgaagttga aatgactcaa attgctatac tacctagtca tcaaggactt
     5161 gggctagcgt ctcaattgat gacgcattta gatagcattg aaagtgacat ctttttagag
     5221 gttagagaat ctaatcatag agcacagggt ttatatcaga agtttggttt taaatttatt
     5281 ggcaaaagac cagactacta tagaaatcct atagaaacag ctttattgat gaaacgggaa
     5341 ggaaaacatg acagataggt atattttagc agttgagagt tcttgtgatg agaccagtgt
     5401 agcgatttta aaaaacgaaa gtaccctctt aagtaatgtc attgcaagtc aggttgagag
     5461 ccacaaacgc tttggtggtg tggtgccaga agtagctagt agacaccatg tcgaagtgat
     5521 tacgacttgc tttgaggatg ctttgcaaga agctggcatc agtgcttcag atttatcagc
     5581 agttgcggta acttatggcc ctggacttgt tggagccttg ttagttggat tagcagctgc
     5641 gaaggctttt gcttgggcaa atcatttacc tttgattccg gttaatcata tggcaggtca
     5701 cttaatggca gcgcgtgaac aaaagccttt agtttatcct ttaatagcac tattggtttc
     5761 aggaggacac acagaattgg tttatgtccc agagccaggg gattatcata ttattggcga
     5821 gacgcgtgac gacgctgttg gtgaggctta tgataaagtt gggcgtgtga tgggcttaac
     5881 ctatccggca ggacgtgaaa ttgatcagtt ggctcataaa ggtcaagata cttatcattt
     5941 ccctcgtgct atgataacag aagaccactt agaattttct ttttcaggac tcaagtccgc
     6001 ttttatcaat cttcatcata atgctaagca aaaaggcaat gaactgattc tagaagattt
     6061 gtgtgcatct tttcaagcag ctgttctaga tattttattg gccaaaacaa aaaaagctct
     6121 gagcagatac cctgcaaaga tgcttgtagt ggctggtggt gtagcggcaa atcaaggatt
     6181 acgagataga ttagcacaag aaattacaca tatagaagtt gtgattccta aactaagact
     6241 ctgtggagat aacgcgggta tgattgcttt agcagcagct attgagtatg acaaacagca
     6301 ttttgctaat atgagcctaa acgcgaagcc tagtttagct tttgaccagt ttccagacag
     6361 ttttgtaata aactagtcac acttgctagt tagtaagatt tataatggat aagaatctcc
     6421 ctttgaaaaa acaacccggt atcttggcat accgggttgt catgatgata agtaattggc
     6481 ttctatggtt actaataatt cagataatta cctgcaaaat aattgtctgc ctaaggtgaa
     6541 gtaagcttac cagctagctt tagtgacgcc aggaagctgt cctttgtgag ctaagtcacg
     6601 gaaattgata cggctaacac caaacttacg catgtaagca tgaggacggc cgtcaatttt
     6661 gtcgcggttc ttcaagcggt ttggatttga atcacgaggc aatttgcgca gtgattcata
     6721 atcgcccttc gcttttaagt cacgacgaag gtcagcgtac tgttcaatca gttggagctg
     6781 cttttggtat ttagcaattt ttgacttttt tgccattgct ttctccttct taatatttaa
     6841 taacctcaga gtcgttatca gcgctctgaa tccacttaac tggtatttaa ctggttaagt
     6901 ggatatataa tcattataca ataaataaaa gcaaaagcaa ataaaaaaaa ttaactagtt
     6961 atctttttta gccataatat caaaaaactc cacatctaag tgtgggagtc aggtattaat
     7021 aactgtttaa aggtgtttaa tgagtgtcgc tttggcaact ttgagattga acttttaagt
     7081 caaataaatc agcaggaacg tagtgtttgg ttgagaccaa gatgtcttta ccattgtaga
     7141 gagtttcttg gattaaaata aaagcactat tttctgatag agtatactta gttgctgtgt
     7201 aattgccagt cttggtatag ccgatttgat tgcaggaatg agaagcttta ccagattcat
     7261 agattttctc ttctaagaaa gtaagaaggg gctcttcttg attgagattt atagcatatt
     7321 tagaaataac ttcaataggg ataaaagata aactataagc aatagcttta ttttggctat
     7381 gataccagcg atcaacaata atcacaacag gagtttcttg ttttagggat gctgtaatgg
     7441 caacagtggg cacttcaatc cgatattcca attcaacctt tgtgatgtca gatgataggc
     7501 ttgcatatat ggggtgttga agatattcga aaccttggtg atatttagtt tcaggagtct
     7561 tgagtataaa atttcccttt cctctgatat tttttatgag atgatcctct tggaggaggg
     7621 ctagggattt acgcagtgtc atccgactga cattgagctg tctagcaagt tcaggttctg
     7681 atggcagttg catgccagga ctatatgtac catcttgaat aagttgaaaa atagtattat
     7741 atacttgcac atgtttaagt tttttgagtt ttttggttaa gtcgttagta gacatatcac
     7801 ttacctcatt aataatcttt agtggagttt aacttgtcta aaaactattt tagcactaac
     7861 agtattcctt gattagaatt gtacccatct atagtgttag tatctcacac tcaggtactt
     7921 tttgtcaaaa aagagatgaa aaacacctta aaaaagcgtt tacaaaatag agtgtgtgtg
     7981 atactataca agtgtatcaa aaattagaca agtaaaatac aggaggtttt taacatgcaa
     8041 aattattcag gtgaagtcgg attgcaatat cacctacaga ttcgtccagg tgatgtaggt
     8101 cgttatgtta tcatgccagg tgatccaaaa cgttgtgcaa aaattgcaga acattttgat
     8161 aatgcagttc ttgtagcaga cagccgtgaa tatgttacgt atacaggtac attaaacggt
     8221 gaaaaagtta gtgttacttc aactggtatt ggtggtccat cagcgtctat tgcgatggaa
     8281 gaattgaaac tctgtggcgc tgataccttt atccgtgttg gaacatgtgg tggtattgac
     8341 cttgatgtta aaggcggaga catcgttatt gcaactggag ctatccgtat ggaagggacc
     8401 agtaaagaat atgctccgat tgaatttcct gctgtagctg acttggaagt gacaaatgct
     8461 ctagttaatg cagctaaaaa gcttggttat acaagtcacg caggagtagt tcaatgtaaa
     8521 gatgcatttt acgggcaaca tgagccagaa cgtatgccag ttagttatga attattaaat
     8581 aaatgggaag catggaaacg tctgggaaca aaagcgtctg aaatggaatc tgcagctctt
     8641 tttgtagcag caagtcatct aggagttcgt tgtggatcag atttccttgt tgttggtaat
     8701 caagaacgca acgctttggg tatggataac ccaatggctc atgatacaga agcagctatt
     8761 caagttgctg ttgaagcact ccgcacactt attgaaaatg ataaatcaca ataagcgagg
     8821 ttttggaagt catcatgcaa tttatttata gtattattgg tattttattg gtattaggaa
     8881 ttgtgtatgc aatttctttc aatcgtaaga gtgtttctct aagtttaatt ggaaaagctc
     8941 ttatcgttca attcattatt gcgctaatct tagtacgtat cccactaggc caacaaattg
     9001 ttagtgttgt ttcaactgga gttactagcg taatcaactg tggtcaagct ggtttaaatt
     9061 ttgtgtttgg gtcattagca gatagtggcg caaaaactgg ttttattttc gctattcaaa
     9121 cgcttggtaa tattgttttc ttatctgccc tagttagtct actttattat gtaggaatcc
     9181 ttggatttgt agtaaaatgg ataggtaagg gcgttggtaa aattatgaaa tcctcagagg
     9241 ttgagagttt tgttgccgta gctaatatgt ttcttggtca aacagacagt ccaattttgg
     9301 ttagcaaata cctaggtcgt atgactgata gtgagataat ggttgtgttg gtatcaggta
     9361 tgggaagtat gtcagtttct attcttggtg gctatattgc attaggcatt ccaatggaat
     9421 atctcttgat tgcttcaaca atggttccta ttggcagtat tctcattgct aaaatcttat
     9481 tgcctcaaac agaacctgtt caaaaaattg atgacattaa gatggataat aaaggtaata
     9541 acgccaatgt gattgatgca atcgctgagg gtgcaagcac aggtgcacaa atggctttct
     9601 caattggggc tagtttgatt gcctttgttg gtttagtttc tttgattaat atgatgttaa
     9661 gtggattggg aattcgctta gaacaaatct tctcatatgt ttttgctcca tttggttttc
     9721 ttatgggatt tgaccacaaa aacattcttc tagaaggaaa ccttcttgga agtaagttga
     9781 ttttaaatga gtttgtttcg ttccaacaat tgggtcacct aatcaaatct ttagattatc
     9841 gtacagcatt ggtagcaact atttcactct gtggttttgc taatttatca agtttaggta
     9901 tttgtgtttc aggtattgct gttctttgcc cggagaaacg tagcacccta gctcgacttg
     9961 ttttccgtgc aatgattggt ggtattgctg taagtatgct tagcgccttt atcgtcggta
    10021 ttgtaactct attctaaacg ttgacaaaag aaagaaggat agtaacgtgg aagtaaaaga
    10081 tattttaaaa acggtagacc atactttgct agcaacaaca gcaacgtggc cagaaatcca
    10141 aacaatttta gatgatgcca tggcttatga aacagcttca gcatgtattc cagcttctta
    10201 cgtcaaaaaa gcagcagaat acgtttcagg taaattagct atttgtactg ttattgggtt
    10261 cccaaatggc tatagtacaa ctgcagcgaa ggtttttgaa tgtcaagatg ctattcaaaa
    10321 tggtgctgat gaaattgaca tggtcattaa tttgacagac gttaaaaatg gggattttga
    10381 tactgttgaa gaagaaattc gtcaaatcaa agccgcatgt caagaccata tcttaaaagt
    10441 tatcgttgag acatgtcaat taactaaaga agaacttatc gaactttgtg gagttgtcac
    10501 acgttcaggt gcagacttta ttaaaacctc tactggtttt tcgacagcag gtgctacatt
    10561 tgaagatgtt gaagtgatgg caaaatatgt cggcgaaggt gttaaaatta aggcagcagg
    10621 tggaatctca tcattggaag atgctaaaac atttattgct ttaggagctt cacgcttggg
    10681 tactagccgt atcatcaaga ttgttaagaa cgaagctaca aaaaccgata gctattaact
    10741 taatcatacc gatttagctt gagactaaaa caaaaaactc atataatagg agttaaaaag
    10801 cctactatct tagtgataat aggctttttt agctatgaag acttccctgc gatccatcct
    10861 gaacaaagag ctgaggtaat attaaaccct ccggtgtgag cattaatatc taagacctct
    10921 ccagcgaaat acaggccagg aactttcttg ctttccaagg ttttagggtt gatttctttt
    10981 aaatctacgc ctcctttggt cacaaatgat ttagccaaag acatttttcc tgtaatgggt
    11041 atctggaggt gtttgagttt atcaagcaat tccttttctt gttttggaga gagttgtttg
    11101 accttttcgg ggtaatcttc tgataaaaaa tcagctactc gttcaggcag taggccttta
    11161 agagcatttt tgatgttttt atcacgttga tcactcagat aagcggttaa gtcatctgtt
    11221 gataagtgtg gcaaaaagtc cagctctgca atttctccac ctttcacaaa agaggacaga
    11281 cgaagagctg caggtcctga aaggccaaag tgggtgaaga gtaagtcgtg tgtgatgacg
    11341 tgtttgtcat aactaagagt aacgtcgtct agggaaatac cttgcaagac tttgtgagga
    11401 aaatcagtta acagagggct ctcagcagct tctaaatcag tcacggtcag tttaaaatgc
    11461 cgtgcaatat catgtccaaa acctgttgaa cccgttgaag ggtaggactt gcctcctgtg
    11521 gtcacaatca atttttggca ggtgaaagtc tggtctgtag atttgaggta gaagaggtca
    11581 tcttgttttt taacggaaac cacttcagtg ctggttagca cctgaccccc aagagccttg
    11641 atttttttct ccagagcatc aatgattgtc cttgatttat ctgttgtggg gaacatccgt
    11701 ccatggtctt cttctttgag tttgacgccg ttttcctcga aaaaggcaat gatgtcatgg
    11761 ttgtcaaact gggaaaaaac actatataaa aagcggccat tcccaggaat gcctgccata
    11821 aggtcatcaa ggttaccact attagtgaca ttacagcgtc caccaccggt tcccgctaat
    11881 tttttaccta agcggcgatt tttttcaatt aagagagttt tatagccata gtagctgctg
    11941 gagatggcgg ccatcatacc agcagggcca cctccaataa taatcgtatc gtattgagtc
    12001 atagccttat tttagcataa aagcgggggg tttaatagtc aggtaaatga actaacctgg
    12061 cataccgttt ttttacgtat ctaaacaatg cctccatgac atcaaaaagc tagaaaaata
    12121 gtccagatga ttaaaattat ctttttatga aaacggttgc ttttttatt
//
LOCUS       DATXQK010000044        11737 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.044, whole genome shotgun sequence.
ACCESSION   DATXQK010000044 DATXQK010000000
VERSION     DATXQK010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11737
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.044"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(52..813)
                     /locus_tag="VC394_001385"
     CDS             complement(52..813)
                     /locus_tag="VC394_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEP5994848.1"
                     /translation="MTKEKMMSFMTIASIALLGSGVVCANDVADPVAGTTDAVVVPVS
                     PQPTTPIEHTGTDTTNKIVDSTKPSEDKVADSTDQLIPPVKSDLETSEDKVTDPTETK
                     PSKEKTEEPVKSNDSEVIPEQPKSEEVKKAEENTGASTTETPKPVTKPVIETPIVTNT
                     GYTVLGTQDGNVWVQTETGNVLKPAIAIGAVKQKDGTVALKTRDGKLEVLPSTGETKT
                     ILAILGGLAILGAIWVGWKENIKKYLTFLKKKSKK"
     gene            complement(838..1395)
                     /locus_tag="VC394_001386"
     CDS             complement(838..1395)
                     /locus_tag="VC394_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994849.1"
                     /translation="MKIRKVIIVMLAVALSLGFYRLYHRQKPSQLIEANQEILVKPIT
                     PNKSKIKVKRNVAKEAKATLEMPEERVNEKEAKQVKSAIVIPIDYLASISSQDDIKVT
                     YDNKLSITNPAMAASIKTMLLAGYHFDLDSLTVYQSNSDNVYQFTILLVTHQQDQLSL
                     VGNYVTGTGQFEFVSLHGTPKNVMF"
     gene            complement(1395..2747)
                     /locus_tag="VC394_001387"
     CDS             complement(1395..2747)
                     /locus_tag="VC394_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEP5994850.1"
                     /translation="MSIPFFIVVIVTIIFSSSQGDCQVTPDLSTQFVTSSDSASQTDW
                     TKKGSIANVTAGKVFHAWVSKGLSGASAAGIVGWVNSEGGFAMIGRAEGHYGNDLRTN
                     SIAFGVKPIGLSYYTTEAGGGIYQFTPYTKYAPLGDPKWENADAMNEFVAKAILAGDW
                     NASMDLTGGNHTFKQMAQMTDPKQATLVWQAYERGSTAHINPFQKQADAQKAYELFGG
                     VKYSYNEIVFHKSFDIFGPLKPGEGESKEVVLSLCESGSYSKGAFGKDRTGKVNYTSY
                     NAWRPEQLPADLKPYALDPRSVGLNYRQAVGWYAIASTGGQCTDLSASLMYALWSKNG
                     SHPTQKAGNGNMVVANWVRTFGGSSDKSPTSGAVFSSAGTSSAGHTGVVSHVFENGDI
                     LIVEQNYASYSGDDGGFGKYSWNYRYVTTAELKNEGYTFYNPAKLGYQIMKSVGTVGK
                     "
     gene            2888..3031
                     /locus_tag="VC394_001388"
     CDS             2888..3031
                     /locus_tag="VC394_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HEP5994851.1"
                     /translation="MGNSSKSNRKEELDLSTFEVLTLMFIAGNFVIALVKLVLELVKS
                     TKK"
     gene            complement(3199..3768)
                     /locus_tag="VC394_001389"
     CDS             complement(3199..3768)
                     /locus_tag="VC394_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994852.1"
                     /translation="MGKHSPMNFSKPSQMTESIDHDVHYDLPGVNEEMESPFQSESDY
                     QSQNSSIIVETPEMDDMIYQSSQKKNISIGNNYSSDKKSLENSFDSPIIPERSEPETD
                     AKERFNPQAEYQRLKAKRQKPLGNRKKSKLEAELGQFTDDASYYKAHGGTAFKRGFNA
                     SKSKELRLKQNLERKATILAELDKLRGHV"
     gene            complement(4342..5514)
                     /locus_tag="VC394_001390"
                     /pseudo
     CDS             complement(4342..5514)
                     /locus_tag="VC394_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5529..8030)
                     /locus_tag="VC394_001391"
     CDS             complement(5529..8030)
                     /locus_tag="VC394_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEP5994853.1"
                     /translation="MVKKLEYPINSIHSNLALRKDKVVIAYYRIPNTPITITDSDKKD
                     SHKDKVAQVIKKLAKYKHFDISLVPKDYLLEEKMSDFIGDLADDVKDLGIDTLSYTVD
                     TLTAEIEIPYQFDWVIGVNLGRDTMSATLKELAVKQLDLIADNVASLLGYTVELDKEW
                     YKEYQDEELLLYGLLSSLKAKRLSEEDLFYYQRMQYLRYIPHAKNEVIANRTLLNVTD
                     TLVKPLSGGFLKLESPYGSSFITILPVGKFNTIFNGFHLGELVQRMSFPVELRFKAEF
                     INKTKLSGTMGRSNTRYDQIMKEAYSTNTVQQDDILMGSYSLKDLMKKVGNKEEIIEY
                     GCFLVVSGSSIKQLRQRRHAVLSYFADMQVNVHEASHDTPYLFQTLLYGQNLQKTTRK
                     WNHLVTSRGFSEMMLFTNTQSGNRIGWYIGRVDNRLSAWDNIGEAIMGSKNLVLFNAT
                     VANKEDVAGKVTKNPHIIITGATGQGKSYLAQMIFLHTAQQNVRVLYIDPKRELRKHY
                     LQVVSDPEYAKQFPLRKHQIEQINFVTLDSAMKENHGVLDPIVILDKEDASSTAKNIL
                     LYLLRNATEVKIVQTTALTEAISEVISRRENGEVVGFNQVIETLCQSDNQDIVDVGHY
                     FKAIIKNSILELAFSDGNVKGLSYEERVTVLEVADLSLPKDGSDHISDHESNSIALMF
                     ALGAFCKHFGERTNEETVEIFDEAWILMQSSEGKAVIKSMRRVGRSKYNVLMLVSQSV
                     HDAENDDDTTGFGTIFAFYEKSEREAILTHVGLEVNEKNLEWIDNMISGQCLYYDVYG
                     NLNMMSVHNIHPDIDPLLKPMKQSVSSHLENKYAS"
     gene            complement(8051..8461)
                     /locus_tag="VC394_001392"
     CDS             complement(8051..8461)
                     /locus_tag="VC394_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017643201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEP5994854.1"
                     /translation="MEEEKYFDYSRGLNAPYWIQEIKTSKGKLLWYFSTPMQLSFFIV
                     FFLILVLMLTVFSAPMQLLNQLTHSISLLLYWFVPYRLSKFYTEYEPQGKKMHTYLWD
                     YLVYLIDYGFNKKAIYQGERVEVLEEIVFEKTNI"
     gene            complement(8464..8733)
                     /locus_tag="VC394_001393"
     CDS             complement(8464..8733)
                     /locus_tag="VC394_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994855.1"
                     /translation="MKVSKVAQVVLTGGGPNVSGLKQFFQGDGIYLISVICAFLVIKN
                     WKNADWLKVGSVLSIYAIVVSLFKGQEILAFLGGMLRWFGIETGL"
     gene            complement(8752..9702)
                     /locus_tag="VC394_001394"
     CDS             complement(8752..9702)
                     /locus_tag="VC394_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEP5994856.1"
                     /translation="MDYLVKIKKFLLRYTKKEKGGKQPKPKEVKQKTANIIVYGILGL
                     LFIVGFFGSLRAIGLSNQVNSLKATVLSSKQQNGKDTKEILDIPRVQYYMNNFVYTYI
                     NYSDDTATKRKEELDNYYSFSTANLVDDVKKERKLQTQRLVSVEQEEDYHIALMRIGY
                     EVDSKSYQMTLAIPFRMANGLLAIVSPPYTLAEDLFQGKSKSFERKTVDQVKSLPKQE
                     MLSIQKFLPVFFDKYALSNEIDLKLLMKHPELMGGNYRVKSLDANNARYYQDKSNQVV
                     QLMVTFEDMVTGGTRSENFTLYLIKSDNGWYVDKLYHYFK"
     gene            complement(9703..10182)
                     /locus_tag="VC394_001395"
     CDS             complement(9703..10182)
                     /locus_tag="VC394_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994857.1"
                     /translation="MIAIIRTHEKGRSQFLCELDIMQFTENQVRDHMIERGIKDDAFF
                     ICGFSDWNVDRIMSLSEVYLLKRCIVGLYDGDDYIVQYLLKKGLSVPAIVTKFYVFLS
                     KDEKEAMGYVLKNVSFDSLIDFWQRSVTWANALNAYIQSGILLNTSKGFYVLKTEGG"
     gene            complement(10190..10671)
                     /locus_tag="VC394_001396"
                     /pseudo
     CDS             complement(10190..10671)
                     /locus_tag="VC394_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000383283.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
     gene            complement(10724..10996)
                     /locus_tag="VC394_001397"
     CDS             complement(10724..10996)
                     /locus_tag="VC394_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994858.1"
                     /translation="MSNTRSEADKKLLVVTQELSELLISHQYDQSWEKAGELNSLLKK
                     REELTLPGYMVDMIQQHLKSYYYQNNMINKAHKSMSAIGHKLQEFH"
     gene            complement(11129..11334)
                     /locus_tag="VC394_001398"
                     /pseudo
     CDS             complement(11129..11334)
                     /locus_tag="VC394_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057932.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(11385..>11737)
                     /locus_tag="VC394_001399"
     CDS             complement(11385..>11737)
                     /locus_tag="VC394_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994859.1"
                     /translation="FISTRNNLELVIDSLVLMIPDREFYYPEIQTGELRDYQIDIYDL
                     IKIGYVGVYEIQKDYEDKLRELADFKRKLLKFGLLMQPLEKQKEIVIRLAAKYHLEKR
                     ILMKKEMFRDEEVD"
BASE COUNT         3700 a         2457 c         1663 g         3917 t
ORIGIN      
        1 tttcgctcct ttcaacaaaa ctaaagccat gaaattaatc atggctttat cttatttttt
       61 acttttcttt ttcaaaaacg ttaaatactt tttgatattt tctttccaac ctacccagat
      121 agctcctaaa atagctaaac ctcccaaaat ggctaagatt gttttagtct ctccagttga
      181 tggtaagact tctaacttcc catctcttgt ttttaaggca actgtaccat ctttttgctt
      241 tacagcacca attgctatcg caggtttcag tacattacca gtttctgttt gaacccaaac
      301 attgccatct tgcgttccca aaaccgtgta gcctgtattc gtaacaattg gtgtctcaat
      361 aacaggtttc gtcacaggtt taggagtttc agttgtacta gcacctgtat tttcttcagc
      421 ttttttcacc tcttctgact taggttgttc tggaatcact tctgaatcat tggacttaac
      481 aggctcttcc gtcttctctt tgcttggctt agtctccgtt ggatctgtta ctttatcctc
      541 gcttgtttct aaatctgatt taactggagg gattagctga tcagtactat ctgccacctt
      601 gtcctcgctt ggcttagtcg agtcaacaat tttattggta gtatctgttc ctgtatgctc
      661 aatcggtgtc gttggttgag gtgagactgg taccaccact gcatccgttg ttcctgctac
      721 tggatctgct acatcattag cacaaaccac cccacttcca agcaaagcaa tacttgcaat
      781 cgtcataaaa ctcatcattt tttcttttgt catcttattt ttcctctttt cgtttaatta
      841 aaacatgaca ttttttggag tgccatgtaa actcacaaat tcaaactgac ccgtaccagt
      901 aacataatta ccaacaagac ttaattgatc ttgctgatga gtcactaata aaatcgtaaa
      961 ttgatacaca ttatcagaat tgctctgata aaccgtcaag ctatctaaat caaagtgata
     1021 ccctgctaaa agcatcgttt taatgcttgc tgccattgct ggattagtaa tggacaactt
     1081 attatcataa gtaaccttaa tatcatcttg acttgatata ctagctaaat aatcaatcgg
     1141 tattacaata gctgatttca cctgtttagc ttctttttcg ttaacacgct cctcaggcat
     1201 ctctaaagtg gccttggctt ctttggcaac atttctctta accttaattt tacttttatt
     1261 cggtgtaatt ggttttacaa gtatttcttg attagcttct attaactgag atggcttttg
     1321 acgatgatac aaccgataaa atcctaatga aagagctact gccagcataa caataataac
     1381 ttttcttatc ttcattattt acctaccgtt cctactgatt tcataatctg ataaccgagt
     1441 ttcgcaggat tgtaaaaagt atatccttca ttttttaatt ctgcagtagt gacatagcga
     1501 tagttccaag agtatttccc aaagccacca tcatcacccg aataagatgc ataattttgt
     1561 tccacaatta aaatatcccc attttcaaaa acatggctga caacacctgt atggccagca
     1621 gaactagtcc ctgcagagga aaatacagct cctgaagttg gtgatttatc agatgaaccg
     1681 ccaaaagtcc taacccaatt agcaacaacc atattaccat tacctgcctt ttgtgttgga
     1741 tggctaccat tttttgacca caaagcatac atcaaactcg cagaaagatc ggtacattga
     1801 ccccctgtcg aagcaatcgc ataccagcca accgcttgtc gataattcaa gccaactgat
     1861 ctgggatcga gcgcataagg ttttaaatct gcaggcaatt gttctggtcg ccaagcattg
     1921 taggaagtat agttgacttt gcctgtacga tctttcccaa aagccccctt agagtaagaa
     1981 cctgattcgc ataatgataa aacgacttct tttgattccc cttcccccgg ctttagtgga
     2041 ccaaaaatgt caaaagattt atgaaatact atttcattat aactatattt aactccaccg
     2101 aataactcat acgctttttg agcatctgct tgcttttgaa atggattaat atgggcagta
     2161 ctaccacgtt cataagcttg ccaaactagc gtagcctgtt tcggatcggt catctgggcc
     2221 atttgcttaa aggtatgatt tcctcctgtc aaatccattg aagcattcca atcaccagct
     2281 agaatggctt ttgcaacaaa ttcgttcatg gcatccgcat tctcccactt aggatcacct
     2341 aaaggggcat atttggtata aggggtaaat tgataaatac ctccacctgc ttctgtcgta
     2401 tagtaactca aaccaattgg tttcactcca aaagctatag aattagttcg taaatcatta
     2461 ccataatgtc cttctgctcg acctatcatg gcaaaaccac cttcggaatt aacccaacct
     2521 acaattcctg cggcagaagc gcctgacaag cctttcgata cccaagcgtg aaagacttta
     2581 cctgctgtga cattcgcaat tgagcctttc tttgtccaat cggtttgtga agcagaatca
     2641 cttgaggtaa caaattgtgt tgataaatca ggagttactt gacaatcacc ttgactgctg
     2701 ctaaatatga tcgtaacgat aactacaata aaaaaaggaa tgctcataag cactcctaga
     2761 catccaagtt tcttcttgat aacattccct ccttcttaaa ctggtcagac agagatacgg
     2821 tatgttataa taaaaaggca gaaaagttaa gacggtggct cttatttctg aaaggtaggt
     2881 ggtgcccatg ggcaattcat caaaatccaa cagaaaggag gagctagatt tgtctacctt
     2941 tgaggtctta acactcatgt ttatagcagg taacttcgtt atcgctctcg tgaagttagt
     3001 ccttgaactg gtaaaatcga caaaaaaata accgtctcaa ctttggcgag ttaacggtta
     3061 ttttttaacg gttattttct gccaccgtct taaacggttc tgcattgggt agttgtttcc
     3121 gcaactgccc ttttctattt tcattataac tcttatctcc aaattaagca actatttgat
     3181 aacatcacaa tacttccttt aaacatgtcc tctaagttta tctaattcag ctaaaatagt
     3241 cgcctttctt tctaaatttt gttttaaccg taactctttt gatttactgg cgttaaatcc
     3301 tcttttaaag gcagtccctc catgtgcttt ataatagctg gcatcatcag taaactgacc
     3361 taattctgct tctaatttag attttttccg atttcctaaa ggtttttgtc gcttagcttt
     3421 tagacgttga tattctgctt gaggattaaa tctctcttta gcgtccgttt ctggttcaga
     3481 cctttcagga ataattggag aatcaaatga attttccaaa gattttttat cagaggaata
     3541 attatttcca atagaaatat ttttcttctg actgctttga taaatcatat catccatttc
     3601 tggagtttca acgataattg aagagttttg agattgataa tcactttctg actgaaatgg
     3661 actttccatc tcctcattaa cacctggtaa atcataatga acatcatgat caattgattc
     3721 agtcatctga cttggtttag aaaaattcat tggggaatgt ttacccaagg gttctgaatt
     3781 atctaatgaa gctccaaaag attggctgta taagttgtct ctttcaaaat cacttacttg
     3841 agagagattg taatcagaag aaaagttact ctcttgatta tcgaaaacac cttgttctgc
     3901 ttgatgtcca gaggtaggat taccattatc agaaacatgc gggacactat ttgtggaaaa
     3961 ttcttgatct cctgcaaaat ctcctaattc tgaaaactga tggtcgaaat tagtttcatt
     4021 agaaaggtca ctataattag aagtgtcatc tcctttgaaa cggtttttaa tggcactaag
     4081 attatcctta gcatgtacaa ggctaccaac accataacca gctcccatca tcgcaagatt
     4141 agaaatatca tgcgccttat catagatatt ttggggtgta actcctaaac tattcgcttt
     4201 ttgagcaacc atatttcctg cttgtgtgac tgcttgtgca gcgtctgctc ctacaattaa
     4261 acttaatacc ttttctttat gtttccaaat ttgccaaaaa ataaccccct gaacaacaat
     4321 agtaataaac aacataaata aaatagattg tcccttaagg ttactgctgc ttaacaaatg
     4381 attgtctgta aatcgagact taataaatgt cgcaatcaat gactgtatat aaaagactat
     4441 taaagttaaa aaacctgtta gagctggaaa ggaaacacct ccaagcatta gcttaagcat
     4501 attaaaagca atatgttgca ttttaggtaa aaaggacacc aataaagcca gtgggaataa
     4561 aaacattaac aatatgacca caatatctgc taaaaaagca attaattgta tcaataataa
     4621 aggtatagct aatatcactg cttcaaaaat tttcaaaata acataaatag acttgatcca
     4681 aatatagtca ctaacagctg acatccacct gttttgttct tctcctttca aagctccgtc
     4741 accaagttta tttgtatact ctaatatttg ttgacttttt ggtattacaa actgatttga
     4801 cttatcatat tttcccagaa ttttgctatt atctaactcc tcttcttttc ctgatttatt
     4861 attatgaaac ttaccattta actggccagt atttacaaaa agataagctg tataagacgt
     4921 tttcataaca taattatcag ctaccgaagc ctcatgagaa atgatttgct tctcatcttc
     4981 tggactcact accgatagat tagttactgc atcactaaaa gaactagcta ccgaatgaac
     5041 catatccaac acatagacac cgcccgaagt tgactgcacc gtcccaaagt aaccgaacgc
     5101 taataacata accgcaaaca aatgaagtaa tcgtcttgaa aaatcacctt ttgaaaaaat
     5161 aaaagctaga aaaatggaaa aagcggataa ggcaattaag agataaagta atgaattcgt
     5221 aaaatctgcg gatttagaga ggttatccca caaaactttg gaactatcaa acacagtctg
     5281 acgataagta ttatacaagc taaagttttc aaaaaatcgt agaataatgg aaaagaaacc
     5341 aacaatcaag ttaagtagaa aaaagggaaa attaacaaaa atatctgtca aagttacttg
     5401 taaccccgtc tcaagacctt ctgtactttc catgtaagcc tttaatgcaa aaatatccac
     5461 atccttgact ttctgaaagg tagaagctat taatccatca tcaatcttat ccatttggcc
     5521 tctcctttct acgaagcgta tttattttct aagtggcttg aaacagactg tttcattggt
     5581 tttaataagg gatcaatatc tggatgaatg ttatggacag acatcatatt gaggttgcca
     5641 taaacatcat aataaaggca ttgtcctgaa atcatgttat caatccactc caggttcttt
     5701 tcgttcactt ccaaaccaac atgagttaaa atagcttccc gttctgattt ttcgtaaaaa
     5761 gcgaaaatag taccaaaacc agtcgtatcg tcatcatttt cggcatcatg tactgattga
     5821 gacactaaca ttaaaacatt gtacttagaa cgaccaacgc ggcgcataga cttgataacg
     5881 gccttacctt ctgatgactg catcagaatc caagcctcat caaaaatttc aacagtttcc
     5941 tcattggttc gttccccaaa gtgcttacaa aaagcaccta aagcaaacat taaagcgatt
     6001 gaattacttt catgatcaga tatatgatct gaaccatctt taggtaacga taagtccgca
     6061 acttctaaaa ctgtcacacg ctcttcatat gataatcctt taacattacc atccgaaaaa
     6121 gccaattcca aaatagaatt cttaataata gctttaaaat aatgtcctac atctacaata
     6181 tcctgattat ctgattgaca taatgtctca atgacttgat taaagccaac gacttcccca
     6241 ttttcacgtc ttgagattac ttcactaatg gcctctgtca aagcagtagt ttgtacaatc
     6301 ttaacttctg tcgcatttct taacagataa agcaatatat ttttagcagt agacgaggca
     6361 tcttctttat ccaagataac gataggatct aaaaccccat ggttttcctt catcgcacta
     6421 tccaatgtca caaaattaat ttgctcaatc tgatgttttc tcagtggaaa ttgtttcgca
     6481 tactcgggat cagacaccac ttgtaaatag tgtttcctta actctctctt aggatcaata
     6541 taaagcactc taacattttg ttgcgcagta tgtaaaaaaa tcatctgtgc aaggtaggac
     6601 ttcccctgac ctgtcgcacc tgtaatgata atatgaggat ttttagttac tttaccagca
     6661 acatcctcct tattcgcaac cgttgcatta aacaaaacta agttcttcga gcccataatc
     6721 gcttcaccaa tattatccca agcagataaa cgattatcga ctcgaccaat ataccagcca
     6781 atcctgttac ctgactgggt atttgtaaat aacatcattt cactaaaccc acgactggtc
     6841 actaagtgat tccatttacg tgttgtcttc tgtaaatttt gaccataaag taacgtttgg
     6901 aataaataag gtgtatcatg actagcttca tgtacattga cttgcatatc cgcaaagtaa
     6961 cttaaaacag catgcctacg ttgccttaat tgtttgatac tagacccaga aaccaccaaa
     7021 aaacaaccgt attcaatgat ttcttcttta tttccaactt tcttcatcaa gtcttttaat
     7081 gaatatgagc ccattagaat atcatcttgt tgcactgtat tcgtggagta ggcttctttc
     7141 ataatttgat cataacgtgt attcgagcgt cccattgttc cacttaactt agttttatta
     7201 ataaattctg ctttaaatcg taactcaaca ggaaaactca tccgctgtac gagttcacct
     7261 aagtggaacc cattgaagat agtattaaac ttcccaactg gtagaatcgt tataaatgaa
     7321 ctaccatacg gactttctaa ctttaaaaaa cctcccgaca ggggcttaac taaagtatct
     7381 gttacgttca acaatgttct attggcaata acttcatttt tagcatgtgg gatatagcgt
     7441 aagtattgca tgcgttgata ataaaataaa tcttcttcag ataatctctt agctttcagt
     7501 gatgataaca aaccatacag caataattct tcatcttggt actctttata ccactcctta
     7561 tcaagttcca cagtgtatcc caacaaagag gcgacattat ctgcaattaa atccaattgc
     7621 tttaccgcaa gttcttttag tgtagcactc attgtatccc tacctaaatt aacaccaata
     7681 acccaatcaa attgataagg tatttcaatc tcagctgtca acgtgtcaac agtgtaactc
     7741 aatgtatcta tacctaaatc cttaacatca tctgccaagt caccaataaa atcagacatt
     7801 ttttcctcta aaaggtaatc ttttggtacc agtgaaatat caaaatgctt gtatttagct
     7861 aattttttga taacctgtgc taccttgtct ttatgactat cttttttatc actatcagta
     7921 atagtgattg gcgtatttgg aatccgatag taggcaataa ccactttatc ttttcgcaaa
     7981 gctaaattac tgtgaatgct attaattgga tattccaact tttttaccat ctgatctcct
     8041 ttcttaatct ttaaatgttt gttttctcaa atacaatttc ttctagtacc tctactcttt
     8101 ctccctgata aatagctttt ttattaaaac cataatctat taaatagaca agataatccc
     8161 aaagataagt atgcatcttt ttaccttgtg gctcatactc cgtataaaat tttgaaagtc
     8221 gatatgggac aaaccagtac agtaacagag aaatagaatg cgttagctga tttagcagtt
     8281 gcataggagc cgaaaagact gtcaacatca atactaaaat caaaaagaaa acaataaaaa
     8341 aggaaagctg cattggtgtt gagaaatacc acaataactt tccttttgac gtttttattt
     8401 cttgaatcca gtaaggggcg tttaggcccc tactgtaatc aaaatatttt tcttcttcca
     8461 tttttataaa cctgtctcaa taccgaacca gcgaagcatc cctcctaaaa aggcaagaat
     8521 ttcttgccct ttaaacagag aaaccacaat cgcataaatt gacagtactg agccaacttt
     8581 tagccaatct gcatttttcc aatttttaat cactaaaaaa gcacagataa cactaataag
     8641 ataaatccca tctccctgaa aaaattgttt tagtcctgaa acattaggtc ctcctcctgt
     8701 taaaacaact tgagctactt ttgatacttt cattaattaa acctcgcttt tttatttaaa
     8761 ataatggtac aacttgtcaa cataccaacc attatctgac ttaatcagat acagcgtgaa
     8821 attctctgac cgtgtacctc ctgttaccat atcttcaaag gtaaccatta attgcacaac
     8881 ttgatttgat ttatcttgat aataccgagc attgttagcg tctaaagatt tgactcgata
     8941 gtttccaccc atcagttcag gatgcttcat tagtaacttc agatctattt cattacttaa
     9001 agcatactta tcaaaaaata caggtaaaaa tttttgtatt gataacattt cttgcttcgg
     9061 taaactcttg acttgatcca cagtttttct ttcaaaactt tttgatttcc cttggaaaag
     9121 atcctccgct aaggtatatg gaggacttac tattgccaat aatccattag ccatacgaaa
     9181 tgggatagcc aacgtcatct gataagattt actatctacc tcatagccta ttctcattaa
     9241 agcaatatga taatcctctt cttgttctac actaaccagt cgttgagtct gaagcttacg
     9301 ctccttttta acatcatcaa caagattagc tgttgaaaaa gaataataat tatctaattc
     9361 ttctttacgt tttgtagccg tatcatcaga atagttgatg taagtataca caaaattgtt
     9421 catgtaatac tgcactctag gaatgtctaa gatttccttt gtatcttttc cgttttgttg
     9481 cttgcttgat aaaaccgtag cttttaaact atttacttga tttgacaaac caattgccct
     9541 taatgagcca aaaaatccta caataaataa taatcctaaa attccataga caataatatt
     9601 ggctgtcttt tgttttactt cctttggttt aggctgcttt cctccttttt ctttctttgt
     9661 atatctcaat agaaactttt ttattttcac taaataatcc atctaacctc cttctgtttt
     9721 taacacataa aatcctttgg atgtatttaa caatattcca ctttgtatgt aagcgtttaa
     9781 agcatttgcc caagttactg accgttgcca aaaatcaatc aatgaatcaa atgatacatt
     9841 tttcaaaaca tatcccattg cttctttttc atcttttgac aaaaagacat aaaactttgt
     9901 cactatggct ggcactgaca agcctttctt taacaaatat tgaacaatgt aatcatcgcc
     9961 atcataaaga ccaacaatac atctctttaa aaggtaaact tccgaaagtg acataatcct
    10021 atcaacattc caatcagaaa aaccacaaat gaaaaaggca tcatccttga tacctctttc
    10081 tatcatgtga tctcttactt gattttccgt aaactgcatg atgtctaact cacataaaaa
    10141 ttgacttcta cctttttcat gagttcttat aattgcaatc attatctact taccctgata
    10201 tttcgacata tccgtagtct gaaaaaccac cttggctttc taagtcaata tctcgaccat
    10261 aagcttcaca atcaaaatag agttccatat tttctttacc aacaccttct gaacctaatt
    10321 cattaaacca gtattcgcct aattctcgtc tagttgtgac atcaggcagt aaacaacaat
    10381 caccaaaatt aaaatcataa tctacaaagt ctgaggcagt ataacctgtt acctgatgca
    10441 ctgcttgaac aagttcacta tcaacattct ctaccagttc aacaaattat taatcatctc
    10501 aatagaatca tgttctttta gtaactgaca atccgaatct gcaataatat actctaaatc
    10561 atcttcttca cgaagcccca aagctaactt aagctcactc atttccacag gaaaacaaac
    10621 taacgtagct ctaccagttt ttatatctct aacaattgca ctaacatcca tttttgacac
    10681 tccttaatta aaaaaggagc aagccattga cttgctctca tccttaatga aattcttgta
    10741 gtttatgacc aatcgctgac atagacttat gagccttatt aatcatatta ttttggtagt
    10801 agtatgattt caaatgttgt tgaatcatgt cgaccatata acctggtaag gtcaattctt
    10861 cacgtttttt aagtaagcta tttaactctc cagctttttc ccatgactgg tcatactgat
    10921 gactaatcag cagttctgat agctcctgag taaccactaa caacttttta tctgcttctg
    10981 aacgtgtatt tgacattttt cttttcctct ttttcttttt ttatactcaa taccaataaa
    11041 tcaacatatg atagttggac acctaatttt catcattttc cctcataaaa aagaacctct
    11101 taaggttcat gaatctaaaa ctaactgttt aagcatctga aatacctaat aaataatttg
    11161 gagtagtatt taatagttta gatagctcaa ttagttttga aaacttggtt ctcgctttcc
    11221 gttctcccaa tttgaatatg ctcctctatt aataccaatt ctatcagcaa gctcttgttg
    11281 tgttaattta ttaagctgtc gttgttttct taaacgatct ccaagttttt ccatttacct
    11341 taccccttat gatttataag ttggatttta aatgatccca gaaattaatc aacttcttca
    11401 tctctaaaca tttccttttt catcagaatt ctcttttcca aatgatattt agcagctaaa
    11461 cgaataacta tctctttttg cttttctaaa ggttgcataa gcaaaccaaa ctttaaaagt
    11521 ttccgtttaa aatcagcaag ttcccttaac ttatcttcgt agtctttttg aatttcataa
    11581 acaccaacat aaccaatctt aatcaaatca taaatgtcta tctgataatc tctaagctca
    11641 cctgtttgaa tctcaggata ataaaattca cgatcaggaa tcattaatac caaagaatca
    11701 atgaccagct ctaaattatt tctcgttgat ataaaat
//
LOCUS       DATXQK010000045        11294 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.045, whole genome shotgun sequence.
ACCESSION   DATXQK010000045 DATXQK010000000
VERSION     DATXQK010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11294
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.045"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(124..1650)
                     /locus_tag="VC394_001400"
     CDS             complement(124..1650)
                     /locus_tag="VC394_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014411840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEP5994860.1"
                     /translation="MPSLFVTFQNRFNEWLAALGEHLQISLLSLMIALLIGVPLAALL
                     SRSKRWSDIMLQVTGVFQTIPSLALLGLFIPLMGIGTLPAVTALVIYAIFPILQNTIT
                     GLNGIDPSLVEAGIAFGMTKWERLKTFEIPIAMPVIMSGVRTSAVMIIGTATLASLIG
                     AGGLGSFILLGIDRNNANLILIGAISSALLAIIFNSLLQYLEKASLRRIMISFGITLL
                     ALLASYTPMALSQFSKGKDTVVIAGKLGAEPDILINLYKELIEDQSDISVELKSNFGK
                     TSFLYEALKSGDIDIYPEFTGTITSSLLRDKPPLSNDPKQVYEDAKKGIAKQDKLTLL
                     KPFAYQNTYAVAMPEKLAKEYQIETISDLKAHADTLKAGFTLEFKDRADGYKGMQSQY
                     GLQLSVATMEPALRYQAIQSGDIQVTDAYSTDAEITKYHLKVLKDDKQLFPPYQGAPL
                     MKTSLLTKHPELKGILNQLAGKITEKEMQDMNYEVSVKGADANKVARDYLLKTGLIQK
                     "
     gene            complement(1643..2371)
                     /locus_tag="VC394_001401"
     CDS             complement(1643..2371)
                     /locus_tag="VC394_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5994861.1"
                     /translation="MIRFNNVSKTFGQTKVLQEQTFQINDREFFVLVGPSGSGKTTLL
                     KMINCLIEPSSGDILLNNVPQKELDLREMRLSIGYVLQQIALFPNLTVAENIAIIPEM
                     KQWSAEEIRQKTEELLDKVGLPAKDYLDRYPSDLSGGEQQRIGIVRAIISHPKILLMD
                     EPFSALDPISRKQLQELMLSLHKEFDMTIVFVTHDIDEAIKLGDRVAILNEGEIVQLD
                     RPEMIKTHPANAFVANLFGGDEHA"
     gene            2794..3483
                     /locus_tag="VC394_001402"
     CDS             2794..3483
                     /locus_tag="VC394_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HEP5994862.1"
                     /translation="MKNAISILALLTSENQLEDMFIRELSHHFSHLFEVTILTSKANI
                     ESNQLSTFQGIFSFHEHDIDLPTLYFKTSQHGQGFLVTESVFDQATAVLSLSQYLTRF
                     YQKFDRHFLQYLPLQARLSDANGNIIVDNHAFNGSFLPTTDKEIEDWILAELRLSDNP
                     CKTFLLPSGSLDHIYMQHYQALKNPQGQLVGVLDTVQDIKPLLNQYLEETGQAIVGWS
                     DVTSGPSILDH"
     gene            complement(3677..4432)
                     /locus_tag="VC394_001403"
     CDS             complement(3677..4432)
                     /locus_tag="VC394_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEP5994863.1"
                     /translation="MGNIALVTGASAGFGRAIVEQLIADGIKVIGASRRLEKLKALET
                     SFGSDNFYPLQMDVTDTKAIDDALTSLPNQWQDITILVNNAGLALGLDKAYEADFENW
                     MTMINTNIVGLIYLTRQLLPHMVSKDDGIIINLGSTAGTIPYPGANIYGASKAFVKQF
                     SLNLRADLAGTHIRVTNIEPGLCDGTEFSKVRFNGDEARVKAIYQGAHAIQPQDIAST
                     VSWVIHQPPHVNVNRIELMPVSQSYGPQPVSRD"
     gene            complement(4559..5554)
                     /gene="pta"
                     /locus_tag="VC394_001404"
     CDS             complement(4559..5554)
                     /gene="pta"
                     /locus_tag="VC394_001404"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEP5994864.1"
                     /translation="MSIRSLFGGLREKILGKNMKIVFPECNDERVVRAAARLKFEGLL
                     EPIILGQSEEVRNLLTKLGFADQDYTIINPNEYADFDKMKEAFVDIRKGKATLEDADK
                     MLRDVNYFGVMLVKMGLADGMVSGAIHSTADTVRPALQIIKTKPGISRTSGVFLMNRE
                     NTSERYVFADCAINIDPTAQELAEIAVNTAETAKIFDIDPKIAMLSFSTKGSGKAPQV
                     DKVREATEIAKGLNPDLALDGELQFDAAFVPETAAIKAPDSAVAGQANTFIFPDLQSG
                     NIGYKIAQRLGMFDAIGPILQGLNKPVNDLSRGSSAEDIYKLAIITAAQAIESQG"
     gene            complement(5558..6463)
                     /locus_tag="VC394_001405"
     CDS             complement(5558..6463)
                     /locus_tag="VC394_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEP5994865.1"
                     /translation="MRFEFVADKRIKVKTLLKSHDVSKGLLAKIKYKGGNILVNGIEQ
                     NAIYLLQVGDVVTIDIPNEEPFEKLEAIPFDLDIVHEDDHFLVINKPIGFASIPSAIH
                     SNTIANFIKAYYVDNHYLDQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQTRSIE
                     KRYFALVSGNGTLPDEGDIIAPIGRSKDSIITRAVDPMGKYAKTSYKVVARYSENVHL
                     VDIKLHTGRTHQIRVHFAHIGFPLLGDDLYGGRLDLGITRQALHCHYLNFKDPFTESD
                     CSYAIHLTDDFDSVIIGLQKKDRGY"
     gene            complement(6460..7296)
                     /locus_tag="VC394_001406"
     CDS             complement(6460..7296)
                     /locus_tag="VC394_001406"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEP5994866.1"
                     /translation="MTQMNYTGKVKRVAIIANGKYQSKRVASKLFSVFKDDPDFYLSK
                     KNPDIVISIGGDGMLLSAFHMYEKELDKVRFVGIHTGHLGFYTDYRDFEVDKLIDNLR
                     KDKGEQISYPILKVAITLDDGRVVKARALNEATVKRIEKTMVADVIINHVKFESFRGD
                     GISVSTPTGSTAYNKSLGGAVLHPTIEALQLTEISSLNNRVFRTLGSSIIIPKKDKIE
                     LVPKRLGIYTISIDNKTYQLKNVTKVEYFIDDEKIHFVSSPSHTSFWKRVKDAFIGEI
                     DS"
     gene            complement(7271..7942)
                     /locus_tag="VC394_001407"
     CDS             complement(7271..7942)
                     /locus_tag="VC394_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEP5994867.1"
                     /translation="MTLDWEEFLDPYIQTVGELKIKLRGIRKQYRKQNRYSPIEFVTG
                     RVKSIESIKEKMILRGVIEENIAQDIQDIAGLRIMVQFVDDVEEVLALLRQRQDMTIV
                     YERDYIRNMKSSGYRSYHVVVEYPVDTIEGQKKVLAEIQIRTLAMNFWATIEHSLNYK
                     YGGDFPEEIKKRLEVTAKIALELDEEMRKIREDIREAQLLFDPVTRNLSDGVGNSDDT
                     DELYR"
     gene            8030..8608
                     /locus_tag="VC394_001408"
     CDS             8030..8608
                     /locus_tag="VC394_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEP5994868.1"
                     /translation="MTNLEIEYKTLLTKNEYNRLLSQMKHVTPVTQTNYYIDTKAFDL
                     KANKMSLRIRTFANSAELTLKVPEKVGNREYNVPLFLEQAKDMIKHGNLPESTALDII
                     ISKGIKPSALVTFGNLTTVRRETVIPIGKLALDYNLYANTKDYELELEVSDALQGKID
                     FDSFLSEHHIAFKYAKSKVARCINTLKKFNDK"
     gene            8748..9728
                     /locus_tag="VC394_001409"
     CDS             8748..9728
                     /locus_tag="VC394_001409"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEP5994869.1"
                     /translation="MTERYADKQIKLFSLTSNLPIAEKIAKAAGIPLGKMSSRQFSDG
                     EIMINIEETVRGDDIYIIQSTSFPVNDNLWELLIMIDACKRASANTVNIVLPYFGYSR
                     QDRVAKPREPITAKLVANMLTKAGIDRVVTLDLHAVQVQGFFDIPVDNLFTVPLFAER
                     YSKLGLSGSDVVVVSPKNSGIKRARSLAEYLDSPIAIIDYAQDDSEREQGYIIGDVSG
                     KKAILIDDILNTGKTFAEAAKILERSGATDTYAVASHGLFAGGAAEVLETAPIKEIIV
                     TDSVKTKNRVPENVTYLSASDLIAEAIIRIHERRPLSPLFSYQPKGKNNA"
     gene            9725..10852
                     /locus_tag="VC394_001410"
     CDS             9725..10852
                     /locus_tag="VC394_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEP5994870.1"
                     /translation="MTYFDNAATTPLSPNVIRAMTAAMQDNFGNPSSIHFYGRRANKI
                     LRECRQAIARNLGASEQQIIVTSGGTESNNMAIKGYALAHQAKGKHLITTTIEHHSVL
                     HTMAYLEERFGFEVTYLPCQNGQINLSDLKQALRDDTILVSIMYANNETGDLLPIKDI
                     GNLLKDHQAAFHVDAVQAVGKLKIIPSELGIDFLSASAHKFHGPKGCGFLYSNGQPID
                     PLLHGGDQEGKRRASTENMLGIIGMAQALTDAMTCLDQSTDHIISLRHHLISLLEGLP
                     YYINQGTHYLPHVLNIGFLGYQNTILLTQLDLAGIAVSTGSACTAGAVNPSHVLAAYY
                     GDDSSRLKESIRISFSDQNSIEDVNQLAQTLKNILGGTDGF"
     gene            10842..11189
                     /locus_tag="VC394_001411"
     CDS             10842..11189
                     /locus_tag="VC394_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEP5994871.1"
                     /translation="MAFEKEIKLKDCKYLYTISPNIKKYTLRDTTFSQTKVGHYQLIR
                     LLEKIPNSGDGFPLKITINKELTGFKLAITDQSGLRLVNIFKTKDNQILQDKFYFLMD
                     SLVEREIFSKTVV"
BASE COUNT         3534 a         2317 c         1898 g         3545 t
ORIGIN      
        1 tttatatttt gtcaaattta aatactattt ttaatttaaa tgttcggttt ttgtatttat
       61 gaattgttac ttcttgaact taagagacga aaaaatgcca gtcctatgga ccaacatttc
      121 tgtttatttc tggattaagc ctgttttcaa cagataatct cgagcgacct tattagcatc
      181 tgctccttta acagatacct cgtagttcat gtcctgcatc tctttttcag taatttttcc
      241 cgctaattga ttaagaatgc cttttaattc tggatgtttt gttaataagg aagttttcat
      301 caaaggagct ccctgataag gtggaaacaa ctgtttatca tcttttaaaa ctttcaaatg
      361 gtatttcgtg atttcagcat ccgtagagta agcatctgtt acttggatat ctcctgattg
      421 aattgcttga taacgaagag ctggctccat cgtcgccaca gatagctgta atccatattg
      481 agattgcatt cccttatagc catctgctct gtccttaaat tccagagtaa aaccagcctt
      541 taaagtatca gcatgcgctt ttaaatcaga aatggtttca atctgatatt ccttagccaa
      601 tttttctggc atagcaacag catacgtatt ttggtaagca aatggcttaa gaagggtcag
      661 tttatcttgc ttagcaatgc cttttttagc atcctcatag acctgcttag ggtcattaga
      721 caaaggtggt ttgtcgcgta aaagacttga tgttatggtt cctgtaaatt caggatagat
      781 atcaatatct ccagatttaa gggcttcata taagaagcta gttttcccaa aattagattt
      841 caactcaaca cttatatctg attgatcttc aattaattcc ttatagagat taatcagaat
      901 atcgggctct gctcccaatt taccggcaat aaccactgta tcttttcctt ttgaaaactg
      961 actaagcgcc ataggagtat acgatgctag caatgcaagt aaggtgattc caaaactaat
     1021 cataatccgt cgcaaggaag ctttctcaag gtactgtaac aaactattga aaataatagc
     1081 tagcagagct gaagaaatag cccctatcag aatcaggtta gcattattac gatcaatccc
     1141 caataagatg aaagagccaa gtccaccggc tcctatcaag gaagctaaag tagctgtgcc
     1201 gataatcatg actgctgacg tccgcacacc tgacataata acaggcattg caattggaat
     1261 ctcaaacgtt tttaaccgct cccatttggt cattccaaaa gctattcctg cttccacgag
     1321 acttgggtca ataccattta atcctgtgat ggtgttttgt aaaatcggaa aaatcgcata
     1381 gataactaaa gctgtcactg caggcaacgt tccaattccc attaaaggga tgaaaagacc
     1441 aagcaaagcc agtgagggaa tggtttgaaa aacacctgtt acctgtaaca taatgtctga
     1501 ccaacgtttg ctgcgactga gaagggctgc taaaggcaca cctattagca aggcgatcat
     1561 aagagataaa agggaaattt gcaggtgttc ccccagagcc gccagccatt cattaaaacg
     1621 gttttggaaa gttacaaaca aactaggcat gctcatcacc tccaaataaa ttggccacaa
     1681 aagcattagc agggtgggtc ttaatcattt ctgggcgatc cagttgcaca atttctccct
     1741 catttaaaat agctacgcgg tctcctaatt taatggcctc atcaatatca tgtgtcacaa
     1801 atacaatcgt catgtcaaat tccttgtgaa gactcaacat taactcttgc aattgtttac
     1861 gtgaaatagg atctaaggct gaaaatggtt catccatcaa tagaatttta ggatgagaaa
     1921 tgatagcacg tacaatacca atacgttgct gttcacctcc cgataaatca cttgggtaac
     1981 gatcaagata atctttagca ggtaggccca ccttatccaa taattcttca gttttttgtc
     2041 taatttcttc agctgaccat tgcttcatct cagggataat cgcaatattt tcagctactg
     2101 tgagattggg gaaaagagca atttgctgca acacgtatcc aatagatagc ctcatctcac
     2161 gtaaatctaa ctctttctgt gggacgttat tcagtaaaat atctccagag cttggttcaa
     2221 taaggcagtt aatcattttt aaaagtgtgg ttttccccga tccgctggga ccaaccagca
     2281 caaaaaattc acggtcatta atttgaaagg tctgctcttg caaaaccttt gtctgaccaa
     2341 aagttttaga aacattatta aaacgtatca ttatttctcc aattcattgg ttaaagtaac
     2401 agcataaaag ctgatgagtt cattcaacct gtctctctgt cattttttct caaaacgttc
     2461 tgagtaacta caaggaatca ctgcatgccc taccattttt agacaaacgc atctactaat
     2521 taaagggact cttaggtatc ctcagtaagg tagaaactac caattctcaa aaactttttc
     2581 tctcattatt ttcttgtcgt ttgttgttga ccattataaa aagtttccca ctataagtca
     2641 aataatgaga ctatttctcc ttacccctta tagaaaaaca ggtcactttg tcagcctatc
     2701 tctccaaaat taccttatca aaaatcctag acttgcctat gatagataag cagattagtt
     2761 gtcttttcta gaaaatacgg ctatcataca attatgaaaa atgccatttc cattttagcg
     2821 ctcttaacat ctgaaaacca acttgaagat atgtttatta gagagctttc ccaccacttc
     2881 tcacaccttt ttgaggtgac tattctcact agcaaagcca acattgagtc aaatcaattg
     2941 tcgactttcc aaggaatttt cagtttccat gagcatgaca ttgacttgcc tacactctat
     3001 ttcaaaacct ctcaacatgg tcagggattt ttagtgacag aaagtgtctt tgatcaagca
     3061 acagcagtct tatcgttaag ccaatacctc actagatttt accaaaaatt tgatagacat
     3121 tttttacagt accttccttt gcaagcaagg ttatctgatg caaatggaaa catcatagtc
     3181 gataatcatg cttttaatgg aagttttttg ccaactactg ataaagaaat agaagattgg
     3241 attcttgctg aattgcgttt atcagataac ccttgtaaaa ccttcctact tccttcgggt
     3301 tcattggatc atatttacat gcagcattat caagctctta aaaatcctca aggtcagctg
     3361 gtgggagttt tggataccgt tcaagacatt aaaccgttat taaaccagta tttagaagaa
     3421 actggacaag ccattgttgg ctggtccgat gtcacatcag gcccttccat tttagatcac
     3481 taaacttgat tttaaatagg gagttcagcc taaatcatca acgcacaaga agcaatcatg
     3541 aacgcttgaa gacctcaaaa ccttcttctt gattgctttt tttgatgact aaatcggtaa
     3601 gtctcactta taaagaataa tccataccag taaaccattt ttctaaagat tattattctt
     3661 atggactcat cccttgctag tcacgactaa ctggttgcgg tccataactt tgcgacactg
     3721 gcatcagctc aatacgatta acattaacat gagggggctg atgaatcacc catgatacag
     3781 tcgacgcaat atcttgaggt tgaatggcat gtgctccttg ataaatggcc ttcacccgtg
     3841 cctcatcacc attaaagcga acctttgaaa attctgtccc gtcacataga ccgggttcaa
     3901 tattggtcac acgtatatgg gtacctgcta aatcagctcg taaattgagt gagaactgct
     3961 taacaaaagc cttgctagcc ccataaatat tagctcccgg atagggaatt gtccctgcgg
     4021 ttgaccctaa attaataata ataccatcat ctttggagac catatgtggt aataattgac
     4081 gtgtgagata aatcaaccca acaatattag tgttaatcat cgtcatccaa ttctcaaaat
     4141 cggcttcata agccttatca agtcccaatg ccaagcctgc attattcact aaaatagtaa
     4201 tatcttgcca ctgatttggt agacttgtca aagcatcatc aatcgctttg gtatctgtga
     4261 catccatctg caaagggtaa aagttgtccg acccaaagct cgtttctaag gccttgagtt
     4321 tctcaagacg acgagaagca ccaataacct ttatcccatc tgcaattaat tgttcgacaa
     4381 tggctctgcc aaaaccggct gaagcccctg ttactaaggc aatatttccc atacatttcc
     4441 tcaccttcaa aaaaaacgag atggtctggc caactcgttt ttaataatgt caattagtct
     4501 taggataact tctaacacat cacttagcgg ttagaatcct atatcaaggg gtcttaagtt
     4561 acccttggct ttcgatagct tgtgctgctg taataatagc taatttgtaa atatcttcag
     4621 cgcttgagcc acgagataaa tcattaacag gtttgttcaa tccttgtaag attggtccaa
     4681 tagcatcaaa cattcctaaa cgttgtgcaa ttttgtaacc aatatttcca gattgtaagt
     4741 cagggaaaat aaaagtattg gcttggccag caaccgctga atctggtgct ttgatagctg
     4801 ccgtttcagg aacaaaggct gcatcaaatt gcaactcacc atcaagtgcc aaatcagggt
     4861 tcaacccttt agcaatttct gttgcttctc ttaccttatc aacttgaggc gctttaccag
     4921 aaccttttgt tgagaagctt aacatagcaa ttttaggatc aatatcaaag attttagctg
     4981 tttcagctgt gttaacagcg atttctgcca attcttgtgc tgttgggtca atattgatag
     5041 cacaatcagc aaacacgtaa cgctcactcg tattttcgcg attcatcaag aaaacacctg
     5101 aagttcttga aatacctggt ttggttttaa tgatttgaag ggctggacga acagtatcag
     5161 ctgttgaatg aatagcacca gataccattc catctgctaa tcccatttta accaacataa
     5221 caccaaagta attcacatca cgaagcattt tatcagcatc ttcaagcgtt gcttttcctt
     5281 tacggatgtc aacaaaagct tctttcattt tgtcaaaatc cgcatattca tttggattaa
     5341 tgatagtata gtcttgatca gcgaagccta attttgtcaa aaggttgcgt acctcttctg
     5401 actgtccaag gataatcggt tctaagagtc cttcaaattt caaacgagca gctgcacgga
     5461 cgacgcgctc atcattacat tctgggaaaa caatcttcat gtttttacca agaatttttt
     5521 ctcttaaacc accgaataaa ctacgaatac tcattcttta ataacctcta tcttttttct
     5581 gtaaacctat gataacgcta tcaaagtcat ctgtcaagtg tattgcatag ctacaatcac
     5641 tttctgtaaa tggatctttg aaattcaaat aatgacaatg taaagcttgc cttgttattc
     5701 ctaagtccaa acgacctccg taaaggtcat cccctaaaag tggaaaacca atatgggcaa
     5761 aatgaactct gatttgatgg gtacgtcctg tatgcaattt gatatccact aaatgcacat
     5821 tttctgaata gcgcgccaca accttatagc tagtcttggc atatttgccc atagggtcaa
     5881 ctgccctggt aataatacta tcttttgaac gcccaattgg agcaataata tctccttcat
     5941 ctggcaacgt cccatttcca gaaaccagag caaaataacg tttctctatt gagcgagttt
     6001 ggagttgctt gtccaacctt gcatgagcat aaccatgctt agcaaataac atcaagccac
     6061 tcgtatctct gtctaaacga gtcacgatat gcacttgttg gtctaggtaa tgattgtcca
     6121 cataatatgc cttgataaag ttagcaatgg tattggagtg gatggcactt ggaatactgg
     6181 caaagccaat gggtttatta atcaccaaaa aatgatcatc ctcgtgaaca atatccaaat
     6241 caaaagggat ggcttcaagc ttttcaaagg gctcttcgtt tggaatgtca atggtgacaa
     6301 catctcccac ttgcaataaa taaatggcat tttgttcaat accattcact aatatattac
     6361 cgcccttata tttgatttta gccaatagtc ctttagagac atcatgactt tttaaaaggg
     6421 tttttacttt tattcgtttg tctgctacaa attcaaatct catgagtcaa tctctccaat
     6481 aaaggcatcc ttgacccttt tccaaaagct cgtatgactc ggagaggaaa caaaatgaat
     6541 tttctcatcg tcgataaaat actccacctt cgtcacattt tttaactgat aggttttatt
     6601 atcaatggaa atggtataaa ttcctaatcg ttttggcact aactcaatct tatctttttt
     6661 gggaataatg attgatgagc ccaaggttct aaagacacgg ttattaagac tggaaatttc
     6721 cgtcaattgc agcgcttcaa tcgtcggatg caagacagca ccacctaaag atttattgta
     6781 ggctgtgctc cctgtcgggg tcgatactga aatcccatca cctcggaagc tttcaaattt
     6841 gacatggtta ataataacat ctgctaccat cgttttttca atacgcttaa ccgtcgcttc
     6901 attcaaagca cgcgctttaa ccacacgacc atcatctaaa gtaatagcaa cttttaaaat
     6961 aggataagag atttgttctc ccttgtcttt tcttaaatta tcaatcaatt tatcaacttc
     7021 aaaatcccta taatcggtat aaaagccaag atgaccggtg tggattccta caaaacgtac
     7081 cttatctaat tctttttcat acatgtgaaa ggcagataag agcatcccat ctccgccaat
     7141 agaaatcaca atatccggat ttttctttga aagatagaaa tcaggatcat ctttaaatac
     7201 ggagaaaagt ttggaggcga cgcgtttact ttggtactta ccatttgcaa taatagcaac
     7261 tcgctttacc ttacctgtat aattcatctg tgtcatcact atttcctaca ccatcactta
     7321 aatttcgtgt cacggggtca aacaataatt gagcctcccg aatatcttct cgaatcttac
     7381 gcatttcttc gtctaattct aaagcaattt tagcagtcac ttcaagacgc tttttaattt
     7441 cttctgggaa gtctccacca tatttataat ttaaagaatg ctcaatggtc gcccaaaagt
     7501 tcattgccaa agtacgaatc tgaatctccg ctaagacctt tttttgacct tcaatagtat
     7561 caacagggta ctcaaccaca acatgataag acctgtagcc actggacttc atattgcgaa
     7621 tataatctcg ttcataaaca attgtcatgt cttgtcgttg acgcaataga gctaagactt
     7681 cttccacatc atcgacaaac tgaaccataa tacgtagtcc agcaatatct tgaatatctt
     7741 gtgctatatt ttcttcaata acaccacgta aaatcatttt ttctttaata ctttcaattg
     7801 acttaacacg tcccgtaaca aattcaatcg gagaataacg attttgctta cgatattgct
     7861 tgcgaatacc ccgtagtttt atttttaatt caccaactgt ttggatataa ggatctaaaa
     7921 attcttccca atctaaggtc atctattgtc tcctttcttg tcaaagccag gataataata
     7981 taaaattgaa ataacaattt attatagcac aaattaaaag gaatttccca tgactaacct
     8041 agaaatcgaa tataaaactc tacttacaaa aaatgagtat aaccgtcttt tatctcaaat
     8101 gaaacatgtg acgcctgtta cacaaactaa ttactatatt gatacaaaag catttgattt
     8161 aaaagctaac aaaatgtcat tacgtattcg aacatttgca aatagcgccg aattaacctt
     8221 aaaagtgcca gaaaaagtgg ggaatcgtga gtacaatgtc cctctttttc ttgaacaagc
     8281 aaaagatatg attaaacatg ggaatcttcc tgaatcaact gctcttgata ttattatttc
     8341 aaaaggtatc aaaccatctg cactggtaac ttttggcaat ttaactaccg ttcgtcgcga
     8401 gacagtgata ccgataggta aattggcctt agactataac ctatatgcca ataccaaaga
     8461 ctatgaatta gaattagaag taagtgacgc tcttcaagga aagattgatt ttgattcttt
     8521 tttatctgaa catcacattg ctttcaaata tgctaaaagc aaagtagctc ggtgtattaa
     8581 caccttaaaa aagttcaacg ataaataatg gctaaaagcg acagaaaaac tgaaattttt
     8641 ctgtttttct gttaaaatag ggatgtcaat taaacacttt tgggtaatgt gactcattaa
     8701 taaaacagtt tacccaattt tattgaagtt aaggagctaa atctatcatg actgaacgat
     8761 atgctgacaa gcaaatcaaa ctgttctcac tcacatcaaa tcttccaatt gctgaaaaaa
     8821 ttgctaaagc tgctggaatc cctcttggaa aaatgtcttc acgtcaattt tccgatggag
     8881 aaattatgat caatattgaa gaaacagtgc gtggagatga tatctatatt attcaatcca
     8941 ctagttttcc tgtcaatgat aatctttggg aattactcat catgattgat gcttgtaaac
     9001 gtgcaagtgc taataccgtc aatattgtat tgccatactt tggttactct cgtcaagacc
     9061 gtgtcgctaa gccccgcgaa ccgattactg ctaaactagt agctaatatg ctgactaaag
     9121 ctggtattga tcgtgtagtg acgcttgact tacatgctgt tcaggtacaa ggtttttttg
     9181 atattccagt ggataacctc tttacagtcc ctctttttgc agaacgttac agtaaattag
     9241 gcttatcagg ttctgatgtt gttgtcgtta gccctaaaaa ttctggaatt aaacgtgcta
     9301 gaagcttggc tgaatatctt gattccccga ttgctattat cgactatgca caagatgatt
     9361 ctgaacgtga acaaggttat attattggtg atgtttctgg taaaaaagcc attttaattg
     9421 atgatatttt aaatactggg aaaacctttg cagaagctgc taaaattctt gaacgctcag
     9481 gcgctactga tacttacgcg gtcgctagcc atggcttatt tgctggtgga gctgctgagg
     9541 tattagaaac agctccaatc aaagaaatta tcgttacaga ttctgttaaa actaaaaatc
     9601 gtgtgccaga aaacgttact tatcttagcg ccagtgattt aatcgcagaa gctattattc
     9661 gtatccatga aagaagaccc ttaagtccac ttttttctta tcaacctaaa ggcaaaaata
     9721 acgcatgact tactttgata acgccgccac cacaccactt agtcctaatg tgattagggc
     9781 gatgacagca gctatgcaag ataactttgg taacccctct agtattcatt tctacgggcg
     9841 ccgagccaat aaaatccttc gtgaatgccg acaagctatc gctagaaact tgggggcaag
     9901 tgaacagcaa attatagtaa cgtccggtgg aactgaaagc aataatatgg ccataaaagg
     9961 ctatgcttta gctcatcaag caaagggcaa acatctcatc acaactacta ttgagcacca
    10021 ctctgtgctt cataccatgg cctatctcga agagcgattt ggttttgagg tcacttattt
    10081 gccttgtcaa aatggacaaa taaatttatc tgacttaaaa caagccttga gagatgacac
    10141 cattttggta agcataatgt atgccaataa cgaaacaggt gacctgttac ctattaagga
    10201 cattgggaat ctgctcaaag accatcaagc tgcttttcat gttgatgccg ttcaagctgt
    10261 tggcaaactc aagattattc ctagcgaact tgggattgac tttttatctg cttctgctca
    10321 caaatttcat ggtcctaaag gatgcggctt tttatacagt aacggacaac caatcgaccc
    10381 tcttcttcac gggggtgatc aagaaggaaa gcgacgcgca agtactgaaa atatgttagg
    10441 tattattggt atggcacaag ctttgactga tgctatgact tgtcttgacc aatcaactga
    10501 tcacattatc agtttacgtc atcacttgat tagcctccta gaaggactac cctactatat
    10561 taatcaaggt actcattatc ttcctcatgt tctcaatatt ggatttttag gctaccaaaa
    10621 tactatttta ctaacacaac ttgacttagc aggcattgca gtatcaactg gttctgcttg
    10681 tactgctgga gctgttaatc caagtcatgt tttagctgcg tactatgggg atgactcttc
    10741 tcgtttaaaa gaatcaattc gtatcagttt ttcagatcaa aatagcattg aagatgttaa
    10801 tcaattagct caaacattaa aaaatatttt aggaggaaca gatggctttt gaaaaagaaa
    10861 ttaagcttaa agactgtaaa tacctttata caatcagtcc aaacattaaa aaatataccc
    10921 taagagatac tacatttagt caaactaaag tagggcacta ccaattaata cgtttactcg
    10981 aaaaaatacc taattcaggg gatggtttcc ctttaaaaat cactattaat aaagaactca
    11041 caggttttaa attagctatc accgatcaat ctggtcttag attagttaat atttttaaaa
    11101 caaaagataa tcaaatcctt caagataaat tttatttctt gatggacagc cttgtagaac
    11161 gtgaaatttt tagcaaaaca gtcgtctaat caactttaaa ttttgttttt ttagtcatat
    11221 cacaatgtca ataaatgatt tcgcttctat tgctattaat tcatgttgtt cattttgact
    11281 gtcataactt actt
//
LOCUS       DATXQK010000046        10619 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.046, whole genome shotgun sequence.
ACCESSION   DATXQK010000046 DATXQK010000000
VERSION     DATXQK010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10619
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.046"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..304)
                     /gene="spej"
                     /locus_tag="VC394_001412"
     CDS             complement(<1..304)
                     /gene="spej"
                     /locus_tag="VC394_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptococcal pyrogenic exotoxin SpeJ"
                     /protein_id="HEP5994872.1"
                     /translation="MKRLIKTIILVIIIFHGYGSVKSDSEKIKDVKLQLNYAYEIRPV
                     DYTNCNIDYLTTHDFYIDVSSYKKKNFSVDSEVESYITTKFTKNQKVNIFGLPYIFT"
     gene            complement(506..643)
                     /locus_tag="VC394_001413"
     CDS             complement(506..643)
                     /locus_tag="VC394_001413"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994873.1"
                     /translation="MLTYHELKKAVANRYIKGDMISITRKLVSSLILSFLIELYEYQE
                     F"
     gene            874..1157
                     /locus_tag="VC394_001414"
                     /pseudo
     CDS             874..1157
                     /locus_tag="VC394_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407356.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1331..1912
                     /locus_tag="VC394_001415"
     CDS             1331..1912
                     /locus_tag="VC394_001415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994874.1"
                     /translation="MKTRPKRFLNLATLSLALLGTTLLMAQPVKADNDTRESQERVDQ
                     RIEDPYDDGKNAGRKDGYESGNHKGISEEPPTKTPEPPTNPYSRDRVKSKKYRDGYQE
                     WYVYGYYEGWHRANDEDKNKESSDHSRDQDLEGTEQTDGQDEDKNKESSDHSRNQDLE
                     GTEQTDTEYSAIISTLYGIAGVVLEYVLSWFSI"
     gene            2572..2808
                     /locus_tag="VC394_001416"
     CDS             2572..2808
                     /locus_tag="VC394_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018364244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEP5994875.1"
                     /translation="MTFEEILPGLKAKKKYVRTGWGGAENYVQLFDTLEVDGKVLQAT
                     PYFLIHVTGEGEGFSMWAPTPCDVLAEDWIEVND"
     gene            2801..3499
                     /locus_tag="VC394_001417"
     CDS             2801..3499
                     /locus_tag="VC394_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEP5994876.1"
                     /translation="MTKVVLVTGCASGIGYAQARYFLRQGYHVYGVDKSDKPDLSGNF
                     HFIKLDLSSELSPLFKVVPSVDILCNTAGILDAYKPLLDVSDEEVEHLFDINFFATVK
                     LTRHYLRRMVEKQSGVIINMCSIASFIAGGGGVAYTSSKHALAGFTRQLALDYAKDQI
                     HIFGIAPGAVKTAMTASDFEPGGLAEWVARETPIGRWTEPDEVAELTGFLASGKARSM
                     QGEIVKIDGGWTLK"
     gene            3575..4534
                     /locus_tag="VC394_001418"
     CDS             3575..4534
                     /locus_tag="VC394_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEP5994877.1"
                     /translation="MKLAVLGTGMIVKEVLPVLQKIDGIDLVAILSTVRSLKTAKDLA
                     KAYHMPLATSEYEAVLGNEEIDTVYIGLPNHLHFAYAKEALLAGKHVICEKPFTMTAG
                     ELDELVVIAKKRKLILLEAITNQYLNNMTFIKEHLDQLGDIKIVECNYSQYSSRYDVF
                     KRGDIAPAFNPKMGGGALRDLNIYNIHFVVGLFGRPKTVQYLANVEKGIDTSGMLVMD
                     YEQFKVVCIGAKDCTAEIKTTIQGNKGSLAVLGATNTLPQVQLSLHGHEAQVVNHNKY
                     DHRMYEEFVAFRDMIDQRDFEKVNQALEHSRAVMAVLERAVHS"
     gene            4867..6204
                     /locus_tag="VC394_001419"
     CDS             4867..6204
                     /locus_tag="VC394_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEP5994878.1"
                     /translation="MEEDYNKREPEKFTQFLRRQKVVFFVAFFGYVCAYLVRNNFKLM
                     SNTIMVQNGWDKAQIATLLSCLTVSYGLAKFYMGALGDRVSLRKLFSISLGASALICI
                     LIGFFNSSMVVLGILLVLCGVVQGALAPASQAMIANYFPNKTRGGAIAGWNISQNMGS
                     ALLPLTIALLTSMGLVVPANGNILLAFLIPGVLVFLFALCCWKLGGDNPESEGLDSLR
                     TMYGDAGESAVASEEEKHNLSYWQLIWKYVFCNPSLLLVAAVNVALYFVRFGIEDWMP
                     IYLSQVANMSEAHIHFAISMLEWVAIPGSLVFAWLAVRYPNKMAKIGAIGLFVLAAIV
                     FVYERLTATGAPNYFLLLVIAGILGSLIYGPQLIVNILTINFVPLNVAGTAIGFVGVT
                     AYLIGNMGANWLMPILADGFGWFWSYIVVAALSAFSAVGYLILAKREEEIIKD"
     gene            6377..7759
                     /gene="glmU"
                     /locus_tag="VC394_001420"
     CDS             6377..7759
                     /gene="glmU"
                     /locus_tag="VC394_001420"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEP5994879.1"
                     /translation="MTNYAIILAAGKGTRMTSDLPKVLHKVSGLTMLEHVFRSVKAIS
                     PEKSVTVIGHKSEMVRAVLADQSAFVHQTEQLGTGHAVMMAETQLEGLEGHTLVIAGD
                     TPLITGESLKSLIDFHVNHKNVATILTATAQDPFGYGRIVRNKDGEVIKIVEQKDANE
                     YEQQLKEINTGTYVFDNKRLFEALKCITTNNAQGEYYLTDVVTIFRANKEKVGAYILR
                     DFNESLGVNDRVALATAETVMRQRITQKHMVNGVTFQNPETVYIESDVEIAPDVLIEG
                     NVTLKGRTHIGSGTVLTNGTYIVDSEIGDNCVVTNSMIESSVLAAGVTVGPYAHLRPG
                     TTLDREVHIGNFVEVKGSHIGEKTKAGHLTYIGNAQVGSSVNVGAGTITVNYDGQNKY
                     ETVIGDHAFIGSNSTLIAPLEVGDHALTAAGSTISKTVPIDSIAIGRSRQVTKEGYAK
                     RLAHHPSRSK"
     gene            7790..8344
                     /locus_tag="VC394_001421"
     CDS             7790..8344
                     /locus_tag="VC394_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEP5994880.1"
                     /translation="MKFEEKTLKRQTVFDGHIFKVVVDDVELPNNLGQSKRELIFHRG
                     AVAVLAITPERKILLVKQYRKAIERVSYEIPAGKLEIGEEGSKLKAAARELEEETAYT
                     GTLTFLYEFYTAIGFCNEKITLFLATDLTQVANPKPQDDDEVIEVLELTYQECMDLVA
                     QGKLADAKTLIALQYYALHFGGEQ"
     gene            8344..8595
                     /locus_tag="VC394_001422"
     CDS             8344..8595
                     /locus_tag="VC394_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994881.1"
                     /translation="MGRPLLTDDIIEKARRMETFESDDAVDFDTKVMTLPEKDDKARI
                     YKSRRIENAKRSQLQSKLNVILIAVMLLIAILVYAIFYL"
     gene            8615..9310
                     /locus_tag="VC394_001423"
     CDS             8615..9310
                     /locus_tag="VC394_001423"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEP5994882.1"
                     /translation="MKIGIIAAMEEELSLLLANLLDAQEHQVLSKTYYTGRFGKHELI
                     LVQSGVGKVMSAMTVAILVEHFKAQAIINTGSAGAVASHLAIGDVVVADRLVYHDVDA
                     TAFGYAYGQMAGQPLYYDCDPQFVAIFKQVLKHEKTNGQVGLIATGDSFVAGQDKIDQ
                     IKTAFSDVLAVEMEGAAIAQAAHTAGKPFIVVRAMSDTAAHDANITFDQFIIEAGKRS
                     AQILMTFLENLPV"
     gene            9461..9802
                     /locus_tag="VC394_001424"
     CDS             9461..9802
                     /locus_tag="VC394_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002993073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994883.1"
                     /translation="MTLKKHYYLLSLLALVTVGAAFNTSQSVSAQVYSNGRYRQGLTD
                     EKSKLQYSKDNAKLQLRNILNGYQSDLGRHYSSYYYYNLRTVMGLSKEQEIEKYYEEL
                     KNKLHDMYNHY"
     gene            complement(9901..10548)
                     /locus_tag="VC394_001425"
     CDS             complement(9901..10548)
                     /locus_tag="VC394_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEP5994884.1"
                     /translation="MTPNKEDYLKCIYEIGEQEPKITNKMVAEKMHVSAPAVSEMIKK
                     MISQGWIVKDKAKGYLLKDKGYALVANLYRKHRLIEVFLIHQLGYNTQEVHQEAEVLE
                     HTVSDTFIDRLDKILDFPDFCPHGGTIPRYGQPLVEMNTTTLNTITELGRFRLSRIHD
                     HFDLIQYLEAHHLSINTELTLTQIDTFAKTYTICYGDKELVIPENIAKQLYVTAL"
BASE COUNT         3219 a         1840 c         2346 g         3214 t
ORIGIN      
        1 gagtaaatat gtacggaaga ccaaaaatat ttactttttg atttttcgta aactttgttg
       61 taatatagct ctcgacctca gaatcaactg aaaaattttt ctttttataa ctggaaacat
      121 caatataaaa atcatgagtg gtcaagtaat caatattaca attcgtataa tctactggtc
      181 tgatttcgta tgcgtaattt aattgtagct taacgtcttt aatcttttca ctatctgatt
      241 ttacacttcc gtacccatga aagattatta taactaaaat tattgttttt attaatcttt
      301 tcatacctct cctcgctcac cccgtgcccc acaaactact ttatcctaaa aaacaataaa
      361 atcaacattg aggacttttt tggttcccat aattgttaac tatgtcattt tagagcaatc
      421 atatagagac taagatataa ttttaccaaa gttttgaagg ctattgaaga gtgactttct
      481 cccttaattt atttaattaa agatactaaa attcttgata ttcataaagc tcaatcagga
      541 aggacaaaat caaagagctt accagttttc tggtaatgct gatcatgtct cctttgatat
      601 acctgttagc tacagctttt ttcaattcgt gatatgttaa catttttatc ctcctactta
      661 atctattcgt aaaaaatctc ttaaaataga catttaaaaa aaagtccatt ataacggata
      721 tgcccgtgtg cgaatacatg ttcgggtaaa tattttaaat caagatgttt gatgtttgtg
      781 tgtgcaaaaa caaaaaaatc gccctcgatt gatgatatga tacgtcttaa gtagacaggc
      841 taaataacca aaatctactc aagaggtatt tttatgccag aaaagaaagg gctgctcgtc
      901 ttaagcatgg tagtgagcct tgtgtccctg cttgatcata tcttcttttt tgctacttat
      961 agttatcttt acttgccact tgtagcaagt agtctagcta ttattagtct ggtgcaaaac
     1021 aaaggaaggc aaaaggtttg gtcgatagct gctttagccg tagcagtagt gacctatacc
     1081 caacggttat tccttatcaa atgcaaagtc cctccctctt atataaacta tcaaatactt
     1141 tcaaaaacag ttcctaaagc tcaaaaaggt tatctaactg ctccaagcag gcactttctg
     1201 tgataaggat agcttgccat ttttttctaa tcatttgata tttaaaatga taaaaaaatg
     1261 aacttgaaaa tgaaagaatg agaggtgtaa actaaaagta ccaaataaac aatgaatacg
     1321 gagtgatttt atgaaaacaa gaccaaagcg ctttttaaat ctagcaaccc ttagcttggc
     1381 ccttttgggc acaaccttac taatggcaca accggtaaag gcggataatg atactaggga
     1441 gtctcaagag cgcgtagacc aaagaataga agatccttat gatgatggaa aaaatgctgg
     1501 gagaaaggat ggttatgaat cagggaatca taagggaatc tctgaagagc ctccaactaa
     1561 aactcctgaa cctccgacta atccttatag ccgtgatagg gtgaaaagca agaagtatag
     1621 agatggttat caggaatggt atgtctatgg atactatgaa ggttggcata gagcaaatga
     1681 tgaggataaa aataaagagt cttctgacca ttctcgagat caggatttag aaggtacaga
     1741 acagacagat ggacaggatg aggataaaaa taaagagtct tctgaccatt ctcgaaatca
     1801 ggatttagaa ggtacagaac agacagatac tgaatattca gcaattatca gtacattgta
     1861 tggaattgct ggagttgtat tggaatatgt gctatcttgg tttagtatct aagatttagt
     1921 tttggttact ggataatggt ttaaatattt attattatct aaaaatggcg tgatcaaatt
     1981 ttgatgattt ggttaatatt gtttagaaca gactgaagac aaagctctta gtaactaaat
     2041 tctaagggct ttgtcttcag tctgaggcac cttacggggt gtcttttatt atttaggtgg
     2101 cttaatgaat tggttaaaat ctttttagtt tagtaaaatt gctatttaag gttagccacc
     2161 tctcgtatgg tcacagttga tgaattatgc tatagtatag acataaaaaa tagttcaaat
     2221 cccttcttgc ctgttggtgg attttgaact gaaagcctct ctgattgcag ttggtaggct
     2281 tttttgatgc tttttcgtac tttttttatt tattacgcta aaaaatgtta tacttgatga
     2341 agaaaaagga gagtttagaa cgttaagata cctcacagca gattgataga ggatatggaa
     2401 aaaggttagc tttcccaaga gatgccgttt gggaatggta taacaataac tggcagaagt
     2461 tataagtaaa acaagaacaa attaactcag atgattttta gacgttccca cgcgtgtgcg
     2521 aattaattgg aaaaaaatga gaaaatacga tatacaaaaa aggagaacaa tatgacattt
     2581 gaagaaattt taccaggttt aaaagccaag aaaaaatatg tacgtacagg ttggggtggc
     2641 gctgaaaatt atgtgcagtt gtttgatacc cttgaagtgg atgggaaagt actacaggcg
     2701 acgccttatt ttctgattca tgttactggt gaaggtgaag gtttttccat gtgggcaccg
     2761 acaccatgtg atgtgttagc agaggattgg atcgaggtca atgactaaag ttgttttagt
     2821 gacgggctgt gctagtggga ttggttatgc acaggcccgc tattttttga gacaagggta
     2881 tcacgtatat ggtgtggata agtcggataa gccggactta agcggtaact ttcactttat
     2941 caaattggat ttatcctctg agttatcccc actctttaaa gtggtcccca gtgttgatat
     3001 tttgtgtaat actgcaggga ttttagatgc ctacaagcca cttttagacg ttagtgatga
     3061 agaagtggaa cacctatttg atattaattt ttttgcgacg gtgaagttaa cacgtcatta
     3121 tcttagacgt atggtggaaa agcagtcagg tgttatcatc aatatgtgct caatagcaag
     3181 ttttatagcg ggtggtggtg gtgttgccta tacgagttct aagcatgcgc ttgcgggctt
     3241 cacgcgtcaa ttggctttgg attacgccaa agatcagatt cacatctttg gtatagcgcc
     3301 aggtgctgtc aaaaccgcta tgacggctag cgactttgag cctggtggct tagcagaatg
     3361 ggtagctcgt gagacgccaa ttgggcgttg gactgaacca gatgaagttg ctgaattaac
     3421 gggatttctt gcgtcaggaa aagcgagatc aatgcaaggt gaaattgtca aaattgacgg
     3481 tggctggact ttgaaatgat cacatgcctt agcttgagct ggcgcagtaa gccctagagg
     3541 tgtgatgagt caaacagtca aaggagatga acaaatgaaa ttagcggttt tggggacagg
     3601 tatgattgtt aaggaagttt tgcctgtctt gcagaaaatt gatggtattg atctagttgc
     3661 tatcttatca acagttagaa gtttaaagac agctaaagac ctcgctaagg catatcacat
     3721 gcctctagct acgagtgaat atgaggctgt cttaggcaat gaggagattg acacggtcta
     3781 tattggactg ccaaatcacc tgcattttgc ctatgctaag gaggctcttc tggctggtaa
     3841 acatgtcatt tgtgaaaagc cctttaccat gacagcagga gaattagatg aactggtggt
     3901 aatcgctaaa aaacgaaagc ttattttgtt agaagcgatt acgaatcagt atttaaacaa
     3961 catgaccttt atcaaagagc atcttgacca acttggcgat atcaaaattg tagaatgcaa
     4021 ttattcacaa tattcttcac gctacgatgt cttcaaacga ggtgatatcg cgccagcttt
     4081 taatcctaaa atgggtggtg gagctttacg tgatttaaac atttacaaca ttcattttgt
     4141 ggtaggatta tttggtcgcc ctaaaacggt tcaatattta gccaatgtcg aaaaaggcat
     4201 tgacacatca ggcatgcttg tcatggacta tgagcagttt aaagtggtgt gtatcggggc
     4261 caaagactgc actgcggaaa tcaaaacaac catccaaggt aataaagggt cactggcagt
     4321 gcttggagca acaaataccc ttcctcaggt tcagttaagt ctacacggtc acgaagcaca
     4381 agtggttaat cacaacaagt atgaccaccg gatgtatgaa gagtttgtcg cttttaggga
     4441 catgattgac caaagagact ttgagaaagt gaatcaggct ttggagcata gccgagctgt
     4501 catggctgtg ttggagcgag ctgttcattc gtgatggtaa tctggtagcc attgcctttt
     4561 gtgcagttgc tatggtaatg gttgaaagac gtgttttcac gagggttaac cccctcgtgc
     4621 ttttttggta aaaataactt gttcaggatt tgggccttct ttgtgagatg agagatgcct
     4681 gacgaattga tttgattaaa ataatgataa agccaaacga tttaagaaat gaccgttcgg
     4741 aaattctgcc ccaaaaggtc tctagaagca gtaaaatcga atttgtaagc gttgacactg
     4801 ctgcataact ctgttacact aagagaatgt catgacaaaa attacagtgt aggagtttaa
     4861 ttgtttatgg aagaggatta taacaaaaga gaacctgaga agtttacgca atttctgcgc
     4921 cgtcaaaagg tcgtcttttt tgtcgcattt tttggttatg tgtgtgctta cttggtacgc
     4981 aacaatttca aactaatgtc aaacactatt atggtccaaa acggttggga caaggctcaa
     5041 attgcgacct tgttatcgtg tttgaccgtt tcttatggct tagcaaaatt ctatatgggg
     5101 gccttagggg atcgtgttag cttgagaaag ctcttttcga tcagcttggg tgcaagcgct
     5161 ttgatttgta ttttaattgg ttttttcaat agttcaatgg tagtgttagg gatactgcta
     5221 gtcttgtgtg gtgttgtaca aggtgccttg gcacctgcct cacaagccat gattgccaat
     5281 tattttccaa ataaaacacg cggcggagcc atcgctggat ggaacatttc tcaaaacatg
     5341 ggatcagccc tcttgccatt aaccatcgcc ttgctcacca gtatgggctt ggtggtacca
     5401 gctaatggca atattttact tgccttttta atccctggtg ttttagtgtt cttgtttgct
     5461 ttgtgttgct ggaagcttgg cggtgataat cctgagtctg agggacttga ctctcttcga
     5521 acgatgtatg gtgatgcggg cgaatcagct gttgccagtg aagaagaaaa gcataacctg
     5581 tcttattggc aactcatctg gaaatacgtt ttttgtaacc cgtcgctctt acttgttgct
     5641 gctgtcaatg tggcccttta ttttgttcgt tttgggattg aagactggat gccgatctac
     5701 ttgtcacaag tagccaatat gtcagaggcc catattcatt ttgcgatttc aatgttagag
     5761 tgggtagcta ttccaggctc gctggtattt gcgtggttag cggttcgtta tcctaataaa
     5821 atggccaaga ttggggctat tgggcttttt gtgttagcgg ctattgtgtt tgtctatgaa
     5881 cgcttgactg ccacaggtgc tccaaactat ttcttgttgc ttgttattgc aggtatttta
     5941 gggtcattga tttatggccc acagttgatc gtgaatattt taacaatcaa ctttgttcct
     6001 ttaaacgttg caggaacagc gattggtttt gtaggagtaa cagcttatct catcggcaat
     6061 atgggagcaa actggctgat gccgattttg gcagatggtt ttggctggtt ttggtcatat
     6121 attgtcgttg cagccttatc tgctttttca gcggttggtt atttaatttt agccaaacgt
     6181 gaggaagaaa tcatcaaaga ttagctaggc aaatgcctaa gaaagacaga cttattgctg
     6241 aagtcctgtc ttttttagtc atcacaagcc gcaagtaaca ctataaaaga caagaaaatg
     6301 ctagggtgtt tcaggaaaaa aagagaaaat atgctataat gagaaggtat ttaaattata
     6361 tagtaaggaa aaacaaatga caaattatgc cattatttta gcagcgggca aagggactcg
     6421 catgacttct gatctcccaa aagttcttca taaagtatct ggactaacca tgcttgagca
     6481 tgtttttcgt agtgtaaaag cgattagtcc tgaaaaatcc gtgacggtta ttggtcacaa
     6541 atcagaaatg gtacgtgctg tcttagcaga tcaatcagcc tttgtccatc aaacagagca
     6601 gttaggaact ggccatgccg tgatgatggc agaaacacaa ctagaaggac tagaagggca
     6661 taccttggtg attgcaggag atactccctt gatcactgga gaaagcctga agagtctgat
     6721 tgattttcat gtgaaccata aaaatgtcgc cactatttta acagctacgg ctcaagatcc
     6781 atttggttat ggtcgtattg ttcgcaacaa agatggcgaa gtgatcaaga ttgttgagca
     6841 aaaagacgcc aatgagtatg agcaacaatt aaaagaaatc aatacaggaa cttatgtgtt
     6901 tgataacaag cgtctctttg aagcgctcaa atgcatcact accaacaatg ctcaaggaga
     6961 gtattacctg actgatgttg tgactatttt tagagcaaac aaagaaaagg taggagccta
     7021 tatcctgaga gacttcaatg aaagtcttgg tgttaatgat cgtgtggcct tagcaacagc
     7081 tgaaacagtg atgcgccagc gcattaccca aaaacatatg gtcaatgggg tgactttcca
     7141 aaatcctgaa accgtttata ttgaaagcga tgttgagatt gctccagatg tccttatcga
     7201 aggaaatgtg accttgaaag ggcgtacaca tatcggctca ggaactgtct tgacgaatgg
     7261 tacttatatt gtggattctg aaattggtga taactgtgtt gttactaatt caatgatcga
     7321 atcatctgtt ttagctgcag gagttacggt tggcccttac gcccaccttc gaccaggaac
     7381 gaccttagat agggaagtcc atatcggtaa ctttgttgag gtcaagggtt ctcacattgg
     7441 tgaaaaaaca aaggctgggc atctgactta tattggaaac gctcaggttg ggtcttcagt
     7501 caatgttggg gctggaacca tcactgtcaa ctacgacggc caaaacaaat atgaaacagt
     7561 cattggagat cacgctttta ttgggagcaa ctcgactctc attgcacctt tggaagttgg
     7621 ggatcacgct ttaacagcag caggttcaac gatttcaaaa acggttccta tcgatagcat
     7681 tgctattggt cgtagccgcc aagtcacaaa agaaggttat gccaagcgcc tagcgcatca
     7741 cccaagtcga agcaaataat tcaagatcta tttagtaagg aggccaggca tgaaatttga
     7801 agaaaaaacg cttaagagac aaacagtttt tgatggtcat atttttaaag ttgttgtgga
     7861 tgatgttgaa cttcccaaca atctggggca atccaagcga gaattaattt ttcatcgtgg
     7921 tgccgttgct gttttggcta ttactcctga acgtaagatt ctccttgtca aacaataccg
     7981 taaggctatt gaacgtgtct catacgagat cccagcgggt aagctagaga ttggtgagga
     8041 aggctcaaag ctaaaagctg ctgcacgtga gctagaagaa gaaacagcct atacggggac
     8101 attgacgttt ttgtatgaat tttatacagc gattggtttt tgcaacgaaa aaatcacact
     8161 tttcttagcg acagacctaa cacaagttgc caatcccaaa cctcaagacg acgatgaagt
     8221 tatcgaggtc ttagagttga catatcaaga gtgcatggac ttagtcgctc aaggtaaact
     8281 tgctgacgct aaaacgttga ttgctttgca atactatgcc ttacattttg gaggtgagca
     8341 gtgatgggaa ggccattgtt gaccgatgat atcatcgaaa aagcgagacg aatggaaaca
     8401 tttgagtcag atgatgctgt agactttgac acaaaagtaa tgacgctacc agaaaaagat
     8461 gataaagcac gcatttataa aagccgtcgg attgaaaatg ccaagcgcag ccaattgcaa
     8521 tctaaattaa atgtgatttt gatagctgtt atgctgttaa ttgccatctt ggtatatgct
     8581 atcttttatc tttaaaaaaa cgtaaggaaa tgatatgaaa ataggaatta ttgctgctat
     8641 ggaggaggag ttgtccctgc tcttagcaaa tcttttggat gctcaagaac atcaggtgct
     8701 ttcaaaaact tactatacgg gtcgttttgg caagcatgag ctcattttgg tacagtcagg
     8761 tgttggaaaa gtgatgtctg ctatgacagt tgcgatcttg gttgagcatt tcaaagccca
     8821 agctattatc aatacaggat cagcaggggc agtcgcttca catttagcga ttggagatgt
     8881 ggtggttgca gatcggttag tgtatcatga tgtggatgct actgcatttg gttatgctta
     8941 tggtcaaatg gctggtcaac cattatacta tgattgtgat ccacagtttg tggctatctt
     9001 caaacaagtc ctaaaacacg aaaagacaaa tggccaagtt ggtttgattg caacaggaga
     9061 cagttttgtt gctggtcagg acaagattga ccagatcaaa acagctttta gcgacgtgtt
     9121 agcagtagaa atggaaggcg cagctattgc tcaagcagca catactgcag ggaaaccctt
     9181 catcgttgtt cgtgcgatga gtgataccgc agcgcatgat gccaatatta cttttgatca
     9241 atttattata gaagctggca agcgttcggc ccagatatta atgacatttt tagaaaactt
     9301 acctgtttaa ctcaccttat cgtgagacaa ttagctggat aatctctatt ttaggagtct
     9361 ggtcacttct tgttttgaaa cctaaaaata agaattgcaa tcacttgacc aaacgctata
     9421 ataggttaag taacgataga ttactaaaag gagtctaaaa atgacactaa aaaaacacta
     9481 ttatcttctc agtctgctag ctcttgtaac ggttggtgct gcctttaaca caagccagag
     9541 tgtcagtgca caagtttata gcaatggaag atatcgtcaa ggtttgactg atgaaaaatc
     9601 aaagctgcaa tatagtaaag acaacgccaa acttcaattg agaaatatcc ttaacggcta
     9661 ccaaagtgac ctagggagac actactctag ctattattac tacaacctaa gaaccgttat
     9721 gggactatca aaagagcaag agattgaaaa atattatgaa gagcttaaga acaagttaca
     9781 tgatatgtac aatcattatt aagttaaagg cttaaaaatg gagactagcc cctcaaaaaa
     9841 agaggccaca ctcatttaaa tttaaatgag tgtggcctct ttttgaggag acgcaagggg
     9901 ttaaagggct gtgacataga gttgcttagc aatattttca gggatgacaa gttccttgtc
     9961 gccataacaa atcgtatagg tttttgcaaa ggtatcaatt tgagttaagg ttagctcggt
    10021 attgatactc aggtgatgtg cttctagata ctggattaaa tcaaagtggt cgtggattct
    10081 gcttaggcga aaccgtccca actccgtgat ggtgttgaga gttgtagtgt tcatttcgac
    10141 aagaggctga ccatagcgag gaatagtccc tccgtgtggg cagaaatctg gaaaatcaag
    10201 gatcttgtct aaacggtcga taaaggtgtc tgagacagtg tgctctaaga cttcagcttc
    10261 ttggtgcact tcttgggtat tatagcccag ctggtgaatc aaaaatacct caatcaagcg
    10321 gtgtttgcgg tagaggttag caacaagagc atagcctttg tcttttagca aatagccttt
    10381 tgctttatct ttgacaatcc agccttgtga aatcattttt ttaatcatct ctgaaacggc
    10441 aggtgccgaa acgtgcatct tttctgcaac cattttattt gtaatcttgg gttcttgttc
    10501 tccaatttca tagatacatt tcaagtaatc ttctttatta ggcgtcatat gtttggcctt
    10561 tctcttattt ataatgatta ttatatcaga atttttagga aaaagcttga caaaaagcc
//
LOCUS       DATXQK010000047        10432 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.047, whole genome shotgun sequence.
ACCESSION   DATXQK010000047 DATXQK010000000
VERSION     DATXQK010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10432
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.047"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            29..766
                     /locus_tag="VC394_001426"
     CDS             29..766
                     /locus_tag="VC394_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEP5994885.1"
                     /translation="MGKEIKVKYFLRRSAFGLVAVSASVLVGSTVSAVDSPIEQPRII
                     PNGGTLTNLLGNAPEKLALRNEERAIDELKKQAIEDKEATTAIEAASSDALEALVDQA
                     DALQSEEAAVVQSDTAASDALEALADQTDALQSEEAAVVKADTAASDALEALADQTDA
                     VQSEEASVVKADTAASDAWEKAATPTALDVKKTKDTTPVVKKEERQNVNTLPTTGEES
                     NPFFTAAALAITVSTGALVVSSKCKEN"
     gene            1004..1546
                     /locus_tag="VC394_001427"
     CDS             1004..1546
                     /locus_tag="VC394_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP5994886.1"
                     /translation="MDIWTKLAVFAFFETPKVILRPFRYEDHWDFYSMVNDTKNLYYV
                     FPEQKTKAASDYLLVHSFIKFPLGQWAIEDKATHQVIGSIRIEHYDAKTRCADIGYFL
                     NYAFWGQGIMTEVVIKLVYLSFHEFGLKTLRIITHLENKASQKVAKKAGFQLKTCFKG
                     SDRNTHKICIYKMYQLTNDR"
     gene            1555..2838
                     /locus_tag="VC394_001428"
     CDS             1555..2838
                     /locus_tag="VC394_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEP5994887.1"
                     /translation="MSKHQDILDYLEKLAIGKKVSVRSISNHLKVSDGTAYRAIKEAE
                     NRGIVETKPRSGTVRIEKKGRVRIDRLTYSEIARISDSEVLAGHAGLGHEFSRFSIGA
                     MTQQNIRRYLVKGGLLIVGDRETIQLLALENHNAILVTGGFPVSKRVIEMANNQRIPV
                     MVTHYDTFTVATMINHALSNIRIKTDLKTVEQVMIPITDYGYLCENSSVEEFNTLIKK
                     TRQVRFPVLDYKRKVIGVVSMRDVVDQLPTTKLTKVMSKNPITARPNTSLANISQKMI
                     FEDLNMLPVTDEENNLLGMITRRQAMENLPNHQPNNPYTYSEQILSNLEETVDYYQVV
                     VEPTMIDSAGNMSNGVISEFLKEISIRALTKKHQKNIIIEQMMVYFLHAIQIEDELKI
                     YPKIITENRRSSTIDIEIFVDDQVIAKAIITTKIN"
     gene            2854..3714
                     /locus_tag="VC394_001429"
     CDS             2854..3714
                     /locus_tag="VC394_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEP5994888.1"
                     /translation="MITLKSAREIEAMDRAGDFLAGIHIGLRDIIKPGVDMWEVEAYV
                     RRRCKEDNVLPLQIGVDGHMMDYPYATCCGLNDEVAHAFPRHYILKEGDLLKVDMVLS
                     EPLDKSIVDVAALDFDNVPEMKKWTRSYTGGLADSCWAYAVGTPSDEIKQLMDVTKEA
                     MYRGIEKAVIGNRIGDIGAAVQEYAESFGYGVVRDLVGHGVGPTMHEEPMVPNYGTAG
                     RGLRLKEGMVLTVEPMINTGTWEIDTDIKTGWAHKTLDGGLSCQYEHQFVITKDGPVI
                     LTSQGEERTY"
     gene            3716..4681
                     /locus_tag="VC394_001430"
     CDS             3716..4681
                     /locus_tag="VC394_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEP5994889.1"
                     /translation="MAEKKWFDKVLSKWQYEPIQVFMRHLQSAEMDLSAIAVAYYLIL
                     TAFPLIVIAANIFPYLNIDIADLLRLMKQNLPKDIFRPASAIVENIFSKPSGSVLGVA
                     TLTGLWTMSRSLTSLQKAINKAYGASQHRDFFIGHLVGLLTSLIILFLLAFALIFSTF
                     SKAAIQVLDKHYHLSDNITTIFLLLIQPITVLIIFVGLMLLYFLLPNVKIKKIRYILP
                     GTLFTSFVMTFLSNLVGNYVVYNVERMVDIKMFGSVMIFIIMLWFIFLARILILGAIF
                     NATYQEMSLGKLEGRSGDMIAILKKTLGNDSDLSPSQSIEDSHTD"
     gene            4792..6075
                     /gene="aroA"
                     /locus_tag="VC394_001431"
     CDS             4792..6075
                     /gene="aroA"
                     /locus_tag="VC394_001431"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012679248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEP5994890.1"
                     /translation="MKLRTNAGPLQGTIQVPGDKSISHRAVILGAVAKGETRVKGLLK
                     GEDVLSTIQAFRNLGVRIEEKDDQLVIEGQGFQGLTAPCQTLNMGNSGTSMRLIAGLL
                     AGQPFSVKMIGDESLSKRPMDRIVYPLKQMGVEISGETDRQFPPLQLQGNRNLQPITY
                     TLPISSAQVKSAILLAALQAKGTTQVVEKEITRNHTEEMIQQFGGRLIVDGKRITLVG
                     PQQLTAQEITVPGDISSAAFWLVAGLIIPGSELLLKNVGVNPTRTGILEVVEKMGAQI
                     VYEDMNKKEQVTSIRVVYSHLKGTIISGGLIPRLIDELPIIALLATQAQGTTCIKDAQ
                     ELRVKETDRIQVVTDILNSMGANIKATADGMIIKGPTVLYGANTSTYGDHRIGMMTAI
                     AALLVKQGQVHLDKEEAIMTSYPTFFKDLERLCHD"
     gene            6068..6559
                     /locus_tag="VC394_001432"
     CDS             6068..6559
                     /locus_tag="VC394_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEP5994891.1"
                     /translation="MTKVLLGFMGVGKTTVSKHLSMHCKDMDAIIEAKIGMSIAAFFE
                     QHGEIAFRTIESQVLKDLLFANDNSIIVTGGGVVVLQENRQLLRKNHQHNILLVASFE
                     TLYQRLKHDKKSQRPLFLKYSKEAFYEFYQQRMVFYEGLSDLVIRVDHRTPEEVANII
                     EGY"
     gene            6767..8218
                     /locus_tag="VC394_001433"
     CDS             6767..8218
                     /locus_tag="VC394_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEP5994892.1"
                     /translation="MTKYPMGGLSHHEELRYFYLLRNLSYLSENEKKEFAFLKSKLEI
                     GRAYAPSKQHYRKSKRQEPYFEDDYYNDYSPNDLLEDDDVNHDSSFVPYGLPIYPKED
                     RYLNKKTKLTARRPIDTPQPIDEDDAFLTESVARCALPRSQKRKHKKKGCMKWFFNIL
                     GLLLMTVLMGLGLMFAKGVFDISTNKANYKPAVSQAFDGQETQDGTNILVLGSDQRVT
                     QGSTDARTDTIMVVNVGNHAKKIKMVSFMRDTLINIPGYSYNDNSYDLKLNSAFNLGE
                     QEDHHGAEYVRRALKHNFDIDIKYYVMVDFETFAEAIDTLFPNGVKIDAKFATVGGVA
                     VDSVEVPDDLRMKNGVVPNQTIEVGEQRMDGRTLLNYARFRKDDEGDFGRTVRQQQVM
                     SAVMSQIKDPTKLFTGSAAIGKIYALTSTNVSFPFVVKNGVSVLGSGKNGVEHVTIPE
                     NGDWVDEYDMYGGQALYIDFDKYQKTLAKLGLR"
     gene            8300..9934
                     /gene="rlmD"
                     /locus_tag="VC394_001434"
     CDS             8300..9934
                     /gene="rlmD"
                     /locus_tag="VC394_001434"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEP5994893.1"
                     /translation="MLKKNDIIQVAISDLSHEGAGVAKHDGFVFFVDNALPEEVIDMR
                     VLKVNKNSGFGKVEAYHYLSPARNADVNLTYLRTGIADLGHLTYEDQLTFKKKQVQDS
                     LYKIAGISDVTVESTIGMTEPLAYRNKAQVPVRRVNGQLETGFFRKHSHDLIPISDYY
                     IQDKEIDRLINFTRDLLRRFDIKPYDETEQTGLLRNIVVRRGHYSGEMMLVLVTTRPK
                     VFRVDQVIEKIVEAFPAVVSIIQNINDKNTNAIFGKDFKTLYGKDTITDSMLGNNYAI
                     SAQSFYQVNTVMAEKLYQTAIAFSDLSKDDIVIDAYSGIGTIGLSFAKTVKAVYGVEV
                     IEAAVRDAQHNAALNGITNAYFVADTAEHAMATWAKDGIKPSVILVDPPRKGLTESFI
                     QASVAMGPQKITYVSCNPATMARDIKRYQELGYKLAKVQPVDLFPQTHHVETVALLSK
                     LGVDKHINIEIEMDELDLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKRKCGIEL
                     REHYNKSKKEKQIIPQCTPEKEEAIMDALRHFKMIE"
     gene            10090..>10432
                     /locus_tag="VC394_001435"
     CDS             10090..>10432
                     /locus_tag="VC394_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEP5994894.1"
                     /translation="MRSYESKEELKNEIKKTFEKYISEFDNIPEELKDKRLEEVDRTP
                     AENLAYQVGWTTLVLKWEEDEKKGIDVKTPSDKFKWNQLGELYQWFTDTYAHKSLYEL
                     KEQLTENVKKIY"
BASE COUNT         3496 a         1731 c         2142 g         3063 t
ORIGIN      
        1 tgcatataaa aataaaaaag gagaaacaat gggaaaagaa ataaaagtga aatacttttt
       61 gcgtagatca gcttttggat tagttgcggt gtcagcatca gtattagtcg gttcaacagt
      121 atctgctgtt gactcaccta tcgaacagcc tcgaattatt ccaaatggcg gaaccttaac
      181 taatcttctt ggcaatgctc cagaaaaact ggcattacgt aatgaagaaa gagccattga
      241 tgaattaaaa aaacaagcta ttgaggataa agaagctacg acagctatag aagcagcaag
      301 ttcagatgcc ttagaagcat tagtggatca agcagacgct ttacaatcag aagaagctgc
      361 agtagttcaa tcagataccg ctgctagtga tgccttagaa gcattggcgg atcaaacaga
      421 cgctttacaa tcagaagaag ctgcggttgt taaagcggat accgctgcta gtgacgcctt
      481 agaagcattg gcggatcaaa cagacgctgt acaatcagaa gaagcttcag ttgttaaagc
      541 agataccgct gctagtgacg cctgggaaaa agcagcaact ccaaccgctt tagatgttaa
      601 gaaaactaaa gatacaacac ctgtagttaa aaaagaagaa agacaaaacg ttaataccct
      661 tcctacaact ggtgaagagt ctaacccatt ctttacagct gctgcgcttg caataacggt
      721 aagtacaggt gcgttagttg taagttcaaa gtgcaaagaa aattagttgc tatttgttct
      781 agcaaatgaa acaagagaat cgaaagattc tctttttctg atatttaaaa atgctacagt
      841 ttataatagc ttgaaagact aaatgttctc aaactcatta gaacaagctt taataaaact
      901 aacccagttg tatcaaagga tagataaaga ctaagactgt tatagaatat ttgcaaaaga
      961 tttgttataa tggacccaat acaggtattg tgaaaggtat aatatggata tttggacaaa
     1021 gcttgcagtg tttgcttttt ttgagactcc gaaagtaata ttacggcctt ttcgctatga
     1081 agatcattgg gatttttaca gcatggttaa cgacactaaa aacctttatt atgtttttcc
     1141 agaacaaaaa actaaggcag caagtgacta tcttttagta catagtttta taaagtttcc
     1201 tttaggtcag tgggcaatag aagataaagc aacccaccaa gtaataggtt ctattagaat
     1261 tgagcattat gatgctaaaa cgcgttgtgc tgatattggc tattttttaa actatgcctt
     1321 ttggggacaa ggaattatga cagaagtcgt aataaaactt gtttatttat cttttcacga
     1381 atttggtctc aaaacgttgc gtattataac tcatttagaa aataaggcca gccagaaagt
     1441 agctaaaaaa gcaggttttc aactaaaaac ctgttttaaa ggaagtgatc gtaatactca
     1501 taaaatctgc atttataaaa tgtaccaact aactaatgat aggtgataat cattatgagc
     1561 aaacatcaag atattttaga ttatttagaa aaacttgcta ttggtaaaaa agtgagtgtt
     1621 agaagcattt caaaccactt aaaagtaagc gatggaacag cctaccgtgc tattaaagag
     1681 gcagaaaatc gaggtattgt cgaaaccaaa ccaagaagtg gaactgttcg tattgaaaaa
     1741 aaaggacggg ttcgtattga ccgtctgacc tattccgaaa ttgctcggat cagtgattcg
     1801 gaagtgctag cgggacatgc tggtttagga catgaattca gtaggttttc aattggtgct
     1861 atgactcaac aaaatattcg tcgttacttg gtcaaaggag ggttattaat agttggggat
     1921 cgtgaaacga ttcaactctt agctcttgaa aaccataatg ccattttggt gacgggcgga
     1981 tttccagtat ccaaacgtgt tattgaaatg gcaaataacc aacgaatccc agttatggta
     2041 actcattatg atacctttac tgtggcaaca atgattaatc acgcgctatc taatattcgt
     2101 attaaaacag atctcaaaac agttgaacag gtaatgatac ctattacaga ttatggttac
     2161 ctttgcgaaa acagctctgt tgaagaattc aatacattaa taaaaaaaac acgacaagtc
     2221 aggtttcctg tattagatta taaaagaaaa gtgattggtg tagttagtat gcgtgatgtg
     2281 gtggatcagt tgccaacaac taagcttacc aaggtcatgt ctaaaaatcc aataacggct
     2341 agaccaaata ctagtcttgc taatattagc cagaaaatga tctttgaaga tttaaacatg
     2401 ttacctgtca ctgacgaaga aaacaattta cttggtatga ttactcgtag acaagcaatg
     2461 gaaaatttgc caaatcacca acccaataac ccctatactt atagtgagca aatactttct
     2521 aaccttgaag aaacggttga ttattaccaa gtagtagttg agccaacaat gattgatagt
     2581 gctggtaaca tgtcaaacgg tgttatttca gagttcttaa aggaaattag tatcagagcc
     2641 ctcacgaaaa agcaccaaaa aaatatcatt atcgaacaaa tgatggtata ctttttacat
     2701 gctattcaaa ttgaggatga attaaaaata tatcctaaaa tcattactga aaatagacga
     2761 agcagcacca ttgatattga aatttttgtg gatgatcaag tgattgctaa agctattatt
     2821 acaacgaaaa taaattagag gagtaacaaa acaatgataa cattaaaatc agcccgtgaa
     2881 atagaagcga tggatagagc aggagacttc ttagcaggta tccatattgg cttaagggat
     2941 attattaagc ctggtgtgga tatgtgggaa gtggaagcat atgttcgtcg ccgttgtaaa
     3001 gaagacaacg ttcttccttt gcaaataggg gttgatggtc acatgatgga ctatccttat
     3061 gcgacctgtt gtggtctaaa tgacgaagta gcacatgcct ttccccgtca ttacattctg
     3121 aaagaagggg atttgcttaa agttgacatg gtactcagtg agcctttgga taaatcaatt
     3181 gttgatgtag cagctttaga ttttgataat gtgcctgaga tgaagaaatg gacaagatct
     3241 tatactggtg gtttagctga ctcttgttgg gcttatgctg ttggaacacc gtctgacgaa
     3301 ataaagcaat taatggatgt caccaaagaa gccatgtacc gtggtattga aaaagcagta
     3361 attggcaatc gtattggtga tattggcgca gctgttcaag aatacgctga aagcttcggt
     3421 tatggtgtcg tgcgtgatct tgttggtcac ggtgttggtc ctacaatgca tgaagagcca
     3481 atggtaccaa actatggaac agcaggtcgt ggacttcgtc taaaagaagg catggtcttg
     3541 actgttgagc caatgattaa tacaggaact tgggaaattg ataccgacat caagacaggt
     3601 tgggctcata aaaccctaga tggtggttta tcttgccaat acgaacacca atttgtgatt
     3661 actaaagatg gtcccgttat tttaacaagt caaggagaag aaagaactta ctaaaatggc
     3721 agaaaaaaaa tggtttgata aggtactgtc caagtggcag tatgaaccca ttcaagtctt
     3781 tatgaggcat ttacaaagtg cagaaatgga tttatctgcc attgcggttg cgtattacct
     3841 tatcttaacg gcctttccat taattgttat tgcagccaat atttttcctt acctcaatat
     3901 tgatattgct gatttgttac gtttaatgaa gcaaaatttg cctaaagata tttttagacc
     3961 ggcttctgca attgtagaaa atattttctc taaaccgtct ggaagtgttt tgggagtagc
     4021 gactttgaca ggattatgga caatgtctcg aagtttgaca tcgctgcaaa aagctattaa
     4081 taaagcctat ggcgcttctc agcatcgaga tttctttatc ggacacttgg ttggtttgtt
     4141 gaccagttta attattttat ttttattagc ctttgcctta atcttttcaa cattttctaa
     4201 agctgctatt caagtgctgg ataagcatta ccatttgagt gataatatca caacgatttt
     4261 cttactcctc attcaaccta ttacagtttt gattattttt gttggcttga tgctactata
     4321 ttttttatta cccaatgtta agattaaaaa aattcgctac attttaccag gaaccctatt
     4381 tacatctttt gtgatgacat ttttgagtaa tctggttggc aattacgttg tgtataatgt
     4441 tgagcggatg gtagatatta aaatgtttgg ttcagttatg atttttatca tcatgctatg
     4501 gtttattttc ttagcaagaa tcctgatttt aggagctata tttaatgcga cctatcaaga
     4561 aatgtccttg gggaaattag aaggtcgtag tggtgatatg atcgctatcc ttaaaaaaac
     4621 acttgggaat gattcagatc tatctccttc acagtctatc gaagatagtc acactgattg
     4681 atacaaaaaa gataataagt cgtggagtga gaaactattt tcttgcttca ttttttatga
     4741 ggataaatag aaaatcattg atcttagcaa atagaaaggc agataaaacg gatgaaattg
     4801 cgaacaaatg caggaccatt acagggaacg attcaagttc caggagacaa gtccattagt
     4861 caccgggcag tcatattagg agctgttgct aaaggggaaa cacgagtaaa aggactctta
     4921 aaaggagaag acgtgctttc cacgattcaa gcctttagga atttgggggt ccgtatcgaa
     4981 gaaaaagatg accagcttgt gatagaaggt caaggttttc aaggattgac cgctccttgt
     5041 cagacactta atatgggaaa ttctggaacc tctatgcgcc tcattgctgg cttattggca
     5101 ggtcaacctt ttagtgtcaa aatgattggc gatgaaagct tatccaaacg tcctatggat
     5161 cggattgttt atccgttaaa gcaaatgggg gttgagatat ctggagaaac agataggcaa
     5221 tttcctccac tacaattgca aggtaatcgt aatttacaac caatcacata tactttgcct
     5281 atctcttctg ctcaggttaa gtcagctatt ttgttagcag ctttgcaagc aaaaggaaca
     5341 acacaggttg ttgaaaaaga gattactcga aaccatacag aagaaatgat tcaacaattt
     5401 ggtggacgat tgatagttga tggtaaacgc attactttgg tgggacccca gcagctcact
     5461 gctcaagaga taactgtccc cggagatatt tctagtgcag ctttttggct tgtcgccggc
     5521 ttaattattc ctggttcgga gctcctttta aagaatgttg gagtcaatcc gacacgaaca
     5581 gggattcttg aagtggttga aaaaatggga gcacagattg tctatgaaga tatgaataag
     5641 aaagagcaag taactagtat tcgagtggta tattctcact tgaaaggaac tatcatatca
     5701 ggaggtctta tccctcgatt gattgatgaa cttcccatca ttgcacttct tgcaacccaa
     5761 gcacaaggaa caacctgtat taaagatgct caagaattac gagtcaaaga aacagatcgt
     5821 attcaagtag taactgatat tcttaacagt atgggagcta atatcaaagc gacagcagat
     5881 ggcatgatta tcaaaggccc tacggtacta tatggggcta acacaagtac ctatggagat
     5941 catcgtattg gtatgatgac agcaattgca gccttgcttg ttaaacaagg acaggtccat
     6001 ttggataaag aagaagccat catgacgtct tatccaacgt ttttcaaaga tttagaaagg
     6061 ctgtgtcatg actaaagtgt tattgggatt tatgggagtg gggaaaacaa cggtttcgaa
     6121 acacctaagt atgcactgta aagatatgga tgctattatt gaagctaaaa tcggtatgtc
     6181 tattgcagct ttttttgaac agcatggaga aatagcattt cgaacaattg aaagtcaagt
     6241 gttaaaagat ctattgtttg caaatgataa tagtatcatc gtaacaggtg gcggtgtagt
     6301 ggttttacaa gaaaatcgac aattactgag aaaaaatcac cagcataata ttttattggt
     6361 agcttctttt gaaactttgt accagcgttt aaaacatgat aaaaagtctc aaagacctct
     6421 atttcttaag tactctaagg aagcatttta tgaattttat caacaacgta tggtatttta
     6481 tgagggactt tcagatttag taatcagagt tgaccatcgt acgcctgaag aagttgccaa
     6541 catcattgag ggttattagt aggtcattgt cgtaatctga tatttatggt tttaaacagg
     6601 tatctgtcta aagcacttct tatggcaagt tggctttatt aaatattagg aaataatctt
     6661 gattattcta taatgattaa aattttgaga ggacaatctg gctaatatag gttataatgg
     6721 tagtattcgt taaaagtgca attgtaatac taagagatga taagatatga caaaataccc
     6781 catgggtggc cttagtcacc atgaagaatt acgttatttt tatctcctaa ggaatttgag
     6841 ttaccttagt gagaatgaaa aaaaagagtt tgctttttta aaatctaaat tggaaattgg
     6901 tcgagcttat gctccttcta agcagcatta taggaagtca aaacggcaag aaccttactt
     6961 tgaagatgac tactataacg actactcacc aaatgattta ctagaagatg atgacgtcaa
     7021 tcatgactct tcttttgttc catatggttt accgatatat cctaaagaag acagatattt
     7081 aaacaaaaaa acaaaactta cagctcgtag acctatagat actccgcagc ctattgatga
     7141 agacgatgct tttttaacag agtctgttgc tcgttgtgct cttcctagat ctcaaaaaag
     7201 aaagcataag aaaaaaggat gtatgaaatg gttttttaac attcttggct tattgctaat
     7261 gactgtgctt atggggttag ggttgatgtt tgcaaaaggg gtttttgata tttctactaa
     7321 taaagctaat tacaaaccag ctgtttccca agcttttgat ggtcaagaaa ctcaagatgg
     7381 tacgaacatt ctggttttgg gttctgacca acgagttact caaggttcta cagatgctcg
     7441 cacagatacc attatggttg tcaatgtcgg caaccatgct aaaaaaataa aaatggtctc
     7501 ttttatgcgt gacactttga ttaacattcc aggatacagt tataatgata attcgtatga
     7561 tcttaaatta aatagtgcat ttaatcttgg agagcaagaa gatcaccacg gagcagagta
     7621 cgttcgacgg gccttaaaac ataattttga tatcgacatt aagtattatg tgatggtaga
     7681 ttttgaaaca tttgcagaag ctattgacac cttgtttcca aatggtgtta agattgatgc
     7741 caaatttgcg acagtaggtg gtgtagctgt tgactccgta gaagttccag atgacctgcg
     7801 aatgaaaaat ggggttgttc ccaaccaaac catcgaagtt ggagaacaac gcatggatgg
     7861 tagaaccttg ctcaactatg ctcgtttccg taaagatgac gaaggagatt tcggacgaac
     7921 cgtccgtcag caacaggtta tgtcagctgt tatgtctcaa atcaaagacc caactaaact
     7981 ctttacaggg tctgcggcta ttggtaaaat ctatgcgctg acttcgacca atgtttcttt
     8041 tccatttgtt gttaaaaatg gtgtttctgt tttaggaagt gggaaaaatg gagttgagca
     8101 cgtgaccatc ccagaaaacg gggattgggt tgacgaatac gatatgtatg gaggacaagc
     8161 cctttacata gattttgata agtatcagaa aacgttagcc aaattaggtc tgcgctaaga
     8221 tatggtataa tggagagtaa ctaaagttgc tcttcttttt tgagcaatgg ttagccctag
     8281 aaaaggaaaa agaataagaa tgttgaaaaa aaatgatatt atccaagttg ctatttcaga
     8341 tttaagccat gagggagcag gtgtcgctaa acacgatggt tttgttttct ttgttgataa
     8401 tgccttgcca gaagaagtga ttgacatgcg tgtcttaaag gtcaataaaa acagcggttt
     8461 tggcaaagta gaagcctatc attacctgtc gccagctaga aatgcagatg tcaatttaac
     8521 ttatttgcga acaggcattg cagaccttgg acatctaact tatgaagacc aactgacttt
     8581 caaaaagaaa caagttcagg acagcctcta taaaattgct ggaatcagtg acgtgacagt
     8641 cgaaagcacg attgggatga cagaaccttt agcttaccga aataaggctc aggttcctgt
     8701 tagacgtgtt aatggtcagc tagaaacagg ctttttccgc aagcattctc atgacttgat
     8761 tcctatttcg gattattata ttcaggacaa agagattgat cgtctcatta attttacccg
     8821 tgatttatta cgccgctttg atattaaacc gtatgacgaa acagaacaaa caggtctctt
     8881 gagaaatatt gttgttcgcc gaggccatta ctctggagaa atgatgctgg tcttagtgac
     8941 tacacgccca aaagttttcc gtgttgacca agtcattgaa aaaatcgttg aagcttttcc
     9001 agcagtggtg tctattattc aaaatattaa tgataagaac accaatgcaa tctttggtaa
     9061 agatttcaaa acgctttacg gcaaggatac cattactgat agcatgcttg gaaacaacta
     9121 tgccatttct gcccagtctt tctaccaagt gaatacggtt atggcagaaa aactctatca
     9181 aacagctata gccttttcag acctttctaa agatgacatt gtgattgacg cctactcagg
     9241 tatcggaacc attggcctat catttgctaa aacagttaaa gctgtttatg gtgttgaagt
     9301 gattgaagca gctgttagag atgctcaaca caatgccgct cttaatggca tcaccaatgc
     9361 ctactttgtc gcagataccg cagaacatgc catggcaaca tgggcgaaag atggcattaa
     9421 accaagtgtt attctggtag acccgccacg aaaaggctta acagaaagtt tcatccaagc
     9481 cagcgtggca atgggaccac aaaaaattac ctacgtttcc tgcaacccag ccaccatggc
     9541 gcgtgacatc aagcgttatc aagagttggg ttacaaactg gccaaagtac agcccgtaga
     9601 tttgtttccc cagacgcatc acgtggagac ggtagcactt ttgtccaaac ttggtgtcga
     9661 taagcatata aatattgaaa ttgagatgga tgagcttgat ttgacaagtg ctgaaagtaa
     9721 ggcaacatat gctcaaatca aagaatatgt ttggaataaa tttgaattaa aagtttcgac
     9781 attatatatt gcacagataa aaaggaaatg tggaatagaa ttacgagaac attacaacaa
     9841 gtctaaaaag gagaaacaaa ttattccaca gtgtacacct gaaaaagaag aagccatcat
     9901 ggatgctttg agacatttca aaatgattga atagaaaaga atgacagcat atcactttct
     9961 gcatttatta cattcctatt tggtataaga acaactatta ttcttttctt gcaaggtatc
    10021 aattagaaaa tagggtcaat ataaagaatg gtaggataat acctatatat aaaccatgga
    10081 ggtaggcata tgaggtctta tgaaagtaag gaagaattaa agaatgagat aaagaaaact
    10141 tttgaaaaat atatttcaga gtttgacaat ataccagaag aactaaaaga taaaagacta
    10201 gaagaagttg ataggacacc agcagaaaat cttgcctatc aagtaggctg gacgacttta
    10261 gtattaaaat gggaagaaga tgagaaaaaa ggtatagatg ttaaaacacc atcagataaa
    10321 ttcaagtgga atcaacttgg agaattatat caatggttta cagataccta tgcccataaa
    10381 tctctatatg aactaaaaga acaactaaca gaaaatgtaa aaaaaattta tc
//
LOCUS       DATXQK010000048        10078 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.048, whole genome shotgun sequence.
ACCESSION   DATXQK010000048 DATXQK010000000
VERSION     DATXQK010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10078
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.048"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..909
                     /locus_tag="VC394_001436"
     CDS             <1..909
                     /locus_tag="VC394_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HEP5994895.1"
                     /translation="SQLIQHSSEIQDLSRLFYLKFGLHLDEYTIAEMFSNHLNDKLEI
                     GCAFEIINQDPISGGRQVTNWIHLLDEMEIKLNLSITNKYEVAVTLHNASVLNEEDIT
                     ANYLLFDYKKSYLNFYQKEHPRIYEAFVTSVEKLMQADNAQVSKELINQLTYCFFITW
                     ENSFLKVNQKEKVRLLVIERSYNSVGNFLKKYIGEFFSITNFDELDCLTIDLVEIEKQ
                     YDVIVTDVMVGKSEELEIFFFYKMIPEAIIDRLNEFLNVSFTDNNVMVKNLEAPSPSK
                     SHSDKEVQKPEKPDNSVKQATSATSS"
     gene            1099..2148
                     /locus_tag="VC394_001437"
     CDS             1099..2148
                     /locus_tag="VC394_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407887.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEP5994896.1"
                     /translation="MSKRNPNKHYSLRKLKTGTASVAVALTVLGTGLANTTDVKAETE
                     HVDVVLSAKEANKVFEERKALEKQARDLGDTINHMSQTISEQSRKIAALKSEAELKNQ
                     QALEALNNKNEQIAKLTNENAQLKEAIEGYVQTIQNASREIAAKQQELAAAKSQLEAK
                     NAEIEALKQQDASKTEEIAKLQSEAATLENLLGSAKRELTDLQAKLDTANTEKEKLQS
                     QAAALEKQLEATKKELADLQAKLAATNQEKEKLEAEAKALKEQLAKQAEELAKLKADK
                     ASGAQKPDTKPGNKEVPTRPSQTRTNTNKAPMAQTKRQLPSTGEETTNPFFTAAAATV
                     IASAGVLALKRKEEN"
     gene            2373..3449
                     /locus_tag="VC394_001438"
     CDS             2373..3449
                     /locus_tag="VC394_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407885.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEP5994897.1"
                     /translation="MARKDTNKQYSLRKLKTGTASVAVAVAVLGAGFANQTEVKAAES
                     PKSTETSANGADKLADAYNTLLTEHEKLRDEYYTLIDAKEEEPRYKALRGENQDLREK
                     EGKYQDKIKKLEEKEKNLEKKSEDVERHYLKKLDQEHKEQEERQKNLEELERQSQREI
                     DKRYQEQLQKQQQLETEKQISEASRKSLSRDLEASRAAKKDLEAEHQKLKEEKQISDA
                     SRQGLSRDLEASREAKKKVEADLAEANSKLQALEKLNKELEEGKKLSEKEKAELQARL
                     EAEAKALKEQLAKQAEELAKLKGNQTPNAKVAPQANRSRSAMTQQKRTLPSTGEAANP
                     FFTAAAATVMVSAGMLALKRKEEN"
     gene            3660..4592
                     /locus_tag="VC394_001439"
                     /pseudo
     CDS             3660..4592
                     /locus_tag="VC394_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561044.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif.; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
     gene            4927..8373
                     /gene="scpA/B"
                     /locus_tag="VC394_001440"
     CDS             4927..8373
                     /gene="scpA/B"
                     /locus_tag="VC394_001440"
                     /EC_number="3.4.21.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021340616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C5a peptidase ScpA/B"
                     /protein_id="HEP5994898.1"
                     /translation="MRKKQKLPFDKLAIALMSTSILLNAQSDIKANTVTEDTPATEQA
                     VEPPQPTAVSEEAPSSSKETNPPQIPDDAEETVADKANDPAPQAPAKTADTPATSKAT
                     IRDLNDPSQVKTLQEKAGKGAGTVVAVIDAGFDKNHEAWRLTDKTKARYQSKEDLEKA
                     KKEHGITYGEWVNDKVAYYHDYSKDGKTAVDQEHGTHVSGILSGNAPSETKEPYRLEG
                     AMPEAQLLLMRVEIVNGLADYARNYAQAIRDAVNLGAKVINMSFGNAALAYANLPDET
                     KKAFDYAKSKGVSIVTSAGNDSSFGGKTRLPLADHPDYGVVGTPAAADSTLTVASYSP
                     DKQLTETATVKTDDHQDKEMPVLSTNRFEPNKAYDYAYANRGTKEDDFKDVEGKIALI
                     ERGDIDFKDKIANAKKAGAVGVLIYDNQDKGFPIELPNVDQMPAAFISRKDGLLLKDN
                     PQKTITFNATPKVLPTASGTKLSRFSSWGLTADGNIKPDIAAPGQDILSSVANNKYAK
                     LSGTSMSAPLVAGIMGLLQKQYETQYPDMTPSERLDLAKKVLMSSATALYDEDEKAYF
                     SPRQQGAGAVDAKKASAATMYVTDKDNTSSKVHLNNVSDKFEVTVTVHNKSDKPQELY
                     YQATVQTDKVDGKHFALAPKALYETSWQKITIPANSSKQVTVPIDASRFSKDLLAQMK
                     NGYFLEGFVRFKQDPKKEELMSIPYIGFRGDFGNLSALEKPIYDSKDGSSYYHEANSD
                     AKDQLDGDGLQFYALKNNFTALTTESNPWTIIKAVKEGVENIEDIESSEITETIFAGT
                     FAKQDDDSHYYIHRHANGKPYAAISPNGDGNRDYVQFQGTFLRNAKNLVAEVLDKEGN
                     VVWTSEVTEQVVKNYNNDLASTLGSTRFEKTRWDGKDKDGKVVANGTYTYRVRYTPIS
                     SGAKEQHTDFDVIVDNTTPEVATSATFSTEDRRLTLASKPKTSQPIYRERIAYTYMDE
                     DLPTTEYISPNEDGTFTLPEEAETMEGGTVPLKMSDFTYVVEDMAGNITYTPVTKLLE
                     GHSNKPEQDGSDQAPDKKPETKPEQDGSGQTPNKKPETKPEKDSSGQTPGKTPQKGQP
                     SRALEKRSSKRALATKASTRDQLPTTNDKDTNRLHLLKLVMTTFFLGLVAHIFKTKRT
                     ED"
     gene            8470..9726
                     /locus_tag="VC394_001441"
     CDS             8470..9726
                     /locus_tag="VC394_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK signal domain/LPXTG anchor domain surface
                     protein"
                     /protein_id="HEP5994899.1"
                     /translation="MRRAENNKHSRYSIRKLSVGVTSIAIASLFLGKVAYAVDSMQPI
                     SSTQGNRANTSKKWYDINEYGLIPVDISFEAAKKDFKEEVEKSLLSEEQQKTYNQKIE
                     AARNKDDLLLTYHSEYSKDIKKHSTSTEPVEASVQETQASVSDSMVTDDSTSVTADSP
                     EKTPSTETPAPSIPEVPEAPASSPESEESSVASSEETSSPETPVAPEAPALSEAPAQP
                     AESEESSVAAPSEETPSPETPAAPETPEEPAAPSPSPESEEPSVAASSEETPSPETPA
                     APETPEEPAAPAQPAESEESSVAATTSPSPSTPAESETQTPPAVTKDSDKPSSAAEKP
                     AASSLVSEQTVQQPTSKRSSDKKEEQEQSYSPNRSLSRQVRAHESGKYLPSTGEKAQP
                     LFIATMTLMSLFGSLLVTKRQKETKK"
BASE COUNT         3730 a         2016 c         1976 g         2356 t
ORIGIN      
        1 tctcaactta tccaacattc ctcagaaatc caggatcttt ctcgcttgtt ctacctcaaa
       61 tttgggttac atctcgacga gtataccata gctgaaatgt tttctaacca tcttaacgac
      121 aagctagaaa ttggctgtgc ttttgagatt atcaatcaag acccaatatc aggaggcaga
      181 caagtaacca actggatcca tctattagat gagatggaaa tcaagctcaa tctcagcatc
      241 accaataaat acgaagttgc cgtaaccctc cataacgctt cggtgttaaa cgaagaagac
      301 attacagcca attatttgct gtttgattat aaaaagagtt acctgaactt ttaccaaaaa
      361 gaacaccctc gtatctatga ggcctttgtg acaagtgtcg agaagctgat gcaggcagat
      421 aatgctcaag ttagcaaaga attgatcaat cagctcactt attgtttctt tattacctgg
      481 gaaaatagtt tcttaaaagt aaatcaaaaa gaaaaagtac gactcctagt gatagagaga
      541 agttacaata gtgtcggtaa ttttttgaaa aagtacatcg gtgagttttt cagcattact
      601 aattttgatg agttagattg tctgacaatt gatctagtag agattgaaaa acagtacgat
      661 gttatcgtga cagatgttat ggtgggtaaa agcgaagagt tagagatttt ctttttctac
      721 aagatgatcc cagaagctat tattgataga ttaaacgaat ttttgaacgt tagtttcaca
      781 gataacaatg ttatggtcaa aaacctcgaa gccccttctc cttcaaaatc tcatagcgac
      841 aaagaggtac aaaagccaga aaagccagac aattctgtta aacaagcaac atcagcaaca
      901 tcatcatagg atttcagacg tcatggtaag aaaaaaagaa tcatttacag cccatagagg
      961 taaggtcaaa agctgaaaac agctcaaaaa aactgacctt taccttttgg ctttttttat
     1021 ttagaataat tttattggag agatgcttaa tctaatttaa gcacaattct tagaaagtta
     1081 agaaataagg agtaaacaat gtctaaaaga aatccaaaca aacactactc actgagaaag
     1141 ttaaaaacag gtactgcatc agtagcagta gctttgacag ttttggggac tggattagca
     1201 aacacaactg atgtaaaggc tgagactgag catgttgatg ttgtgctttc agcaaaagaa
     1261 gctaacaaag tattcgaaga gcgcaaagcc ttggaaaaac aagcgcgtga tttgggtgac
     1321 actattaatc acatgtcaca aaccattagt gagcaaagcc gcaagattgc agcattaaag
     1381 tctgaagcag aacttaaaaa ccaacaagct cttgaagctt taaataataa aaatgagcag
     1441 attgctaaat taaccaacga aaacgcacag ttaaaagaag ccattgaagg ttatgtgcaa
     1501 actatccaaa acgctagccg tgaaatcgca gcaaaacaac aagaacttgc agctgcaaaa
     1561 agccagttag aggcaaaaaa tgctgagatt gaggcattga aacaacaaga tgcctctaag
     1621 actgaggaaa ttgctaaatt gcaatcagaa gcagcaactt tagaaaacct cctaggttca
     1681 gctaagcgtg aattgactga tctgcaagct aagctagata cagctaacac tgaaaaagaa
     1741 aagctccaat cacaagcagc agccctagaa aaacaactag aagcaactaa aaaagagtta
     1801 gctgatttac aggctaaatt agcagcaacc aaccaagaaa aagaaaagtt agaagctgaa
     1861 gcaaaagctc ttaaagagca attagcaaaa caagctgaag agcttgctaa gctaaaagca
     1921 gataaagctt caggagctca aaaaccagat actaaacctg gcaataaaga ggttccaaca
     1981 agaccgtcac aaacaagaac aaacactaat aaagctccta tggcgcaaac aaagagacaa
     2041 ttaccgtcaa caggcgaaga aacaaccaac ccattcttca ctgcagcagc tgcaacagtg
     2101 atcgcatctg caggcgtact tgccctaaaa cgcaaagaag aaaactaagc ccaatccaca
     2161 ctatcttttc tagcccaaga aaaaaacaaa aaaagaggaa gaccattcct ctttttttga
     2221 acggttaaac agcaaaaagg tcaaaaaggt actaaagtct caaaaacctg gtctttacct
     2281 tttaccgctt attctttaga atagaattat tagagagaag tcttagaaaa atgaggctaa
     2341 ttccctaaag atgaaaaaat aaggagcaaa taatggctag aaaagatacg aataaacagt
     2401 attcgcttag aaaattaaaa acaggtacag catccgtagc ggtcgctgtg gctgttttag
     2461 gagcaggctt tgcaaaccaa acagaagtta aggctgcgga gtctccaaaa agtactgaga
     2521 cttctgctaa tggagctgat aaattagctg atgcatacaa cacattgctt actgaacatg
     2581 agaaactcag agatgagtat tatacattaa ttgatgctaa agaagaagaa cctaggtata
     2641 aagcattgag aggcgaaaat caagatcttc gggaaaaaga aggaaaatac caggataaaa
     2701 taaaaaaatt agaagaaaaa gagaaaaatt tagaaaaaaa atcagaagat gtagaacgtc
     2761 actatcttaa aaaactagat caagaacata aagaacaaga agaacgtcaa aaaaatctag
     2821 aagaactcga acgtcaaagt caacgagaaa tagacaagcg ttatcaagaa caactccaaa
     2881 aacaacaaca attagaaaca gaaaagcaaa tctcagaagc tagtcgtaag agcttaagcc
     2941 gtgaccttga agcgtctcgt gcagctaaaa aagaccttga agctgagcac caaaaactca
     3001 aagaggaaaa acaaatctca gacgcaagcc gtcaaggcct aagccgtgac cttgaagcgt
     3061 ctcgcgaagc taagaaaaaa gtagaagcag acttagcaga agcaaatagc aaacttcaag
     3121 cccttgaaaa actaaacaaa gagcttgaag aaggtaagaa attatcagaa aaagaaaaag
     3181 ctgagttaca agcaagacta gaagctgaag caaaagctct taaagagcaa ttggctaaac
     3241 aagctgaaga acttgctaaa ctaaaaggca accaaacacc aaacgctaaa gtagccccac
     3301 aagctaaccg ttctagatca gcaatgacgc aacaaaagag aacgttaccg tcaacaggcg
     3361 aagcagctaa cccattcttt acagcagcag ctgcaacagt aatggtatct gcaggtatgc
     3421 ttgctctaaa acgcaaagaa gaaaactaag ctattagact gatgctaaag ttaagagaga
     3481 atcaaatgat tctctctttt tgagtggcta agtaactaac aatctcaatt agaccaaaaa
     3541 atgggaatgg ttcaaaaagc tggcctttac tccttttgat taaccatata taagtaaaaa
     3601 cattaggaaa ataatagtaa tattaagttt gtttcctcaa taaaatcaag gagtagataa
     3661 tggctagaca acaaaccaag aaaaattatt cactacggaa actaaaaacc ggtacggctt
     3721 gagtagccgt tgctttgacc gttttgggtg caggttttgc aaaccaaacg gaagtaagag
     3781 ctgaaggggt aaacccgact acgaacttgc cagagaaggc taaatatgcc gcattgaaag
     3841 atgagaatac tggtttacgt ggtgatcaga aaaaattagt aaaaaaactt gaagaagaac
     3901 aagagaagag caaaaatcta gaaaagcaaa aacaggagtt agaaaaccaa gcccttaact
     3961 ttcaagacgt aattgaaact caggaaaaag aagatctcaa aacaacttta gctaagacta
     4021 ctaaagaaaa tgagatttca gaagctagcc gtaaagggtt aagccgagac ttagaagctt
     4081 ctcgtgcagc taaaaaagag ctagaagcta agcaccaaaa attagcagaa gaacatcaag
     4141 tttcagagac tagccgtaag ggtctaagcc gtgaccttga agcatcacgt gaagctaata
     4201 agaaggttac atctgagtta acacaagcaa aagctcaact ctcagcgctt gaagaaagta
     4261 agaaattatc agaaaaagaa aaagctgagt tacaagcaaa actagatgca caaggaaaag
     4321 ccctcaaaga acaattagca aaacaaactg aagagcttgc aaaactaaga gctgaaaaag
     4381 cggcaggttc aaaaacacct gctaccaaac cagctaataa agaaagatca ggtagagctg
     4441 ctcaaacagc tacaagacct agccaaaata aaggaatgag atcacaatta ccgtcaacag
     4501 gcgaagcagc taacccattc tttacagcag cagctgcaac agtgatggta tctgcaggta
     4561 tgcttgctct aaaacgcaaa gaagaaaact aagcctttag aacttggttt ttgtaacggt
     4621 gcaatagaca aaagtaagca aggccaaaaa ctgagaaagt cctaaaaagc tggcctttac
     4681 ccctcaaaat taatgtttta taataaagat gttagtaata taattgataa atgagataca
     4741 tttaatcatt atggcaaaag caagaaaaat agctgtatca tatgcaaata acccctgttt
     4801 gctctttaaa aaagacgtta tccttatttc tccacgcaca gatggacagc taggagagaa
     4861 tcgtttgatt ctctcttttc ttaatggtca taaagacaaa gtctctcatc atgaaaggac
     4921 gacacattgc gtaaaaaaca aaaattacca tttgataaac ttgccattgc gctcatgtct
     4981 acgagcatct tgctcaatgc acaatcagac attaaagcaa atactgtgac agaagacact
     5041 cctgctaccg aacaagccgt agaaccccca caaccaacag cggtttctga ggaagcacca
     5101 tcatcatcaa aggaaactaa ccctccacaa attcctgatg acgcagaaga aacagtagca
     5161 gataaggcta atgatccagc ccctcaagct cctgctaaaa ctgctgatac accagcaacc
     5221 tcaaaagcga ctattaggga tttgaacgac ccttctcagg tcaaaaccct gcaggaaaaa
     5281 gcaggcaagg gagctgggac tgttgttgca gtgattgatg ctggttttga taaaaatcat
     5341 gaagcgtggc gtttaacaga caaaaccaaa gcacgttacc aatcaaaaga agatcttgaa
     5401 aaagctaaaa aagagcacgg tattacctac ggcgagtggg tcaatgataa ggttgcttat
     5461 taccacgact atagcaaaga tggtaaaacc gctgtcgatc aagagcacgg cacacacgtg
     5521 tcagggatct tgtcagggaa tgctccatct gaaacgaaag aaccttaccg cctagaaggt
     5581 gcgatgcctg aggctcaatt gcttttgatg cgtgtcgaaa ttgtaaatgg actagcagac
     5641 tatgctcgta actacgctca agctatcaga gatgctgtca acttgggagc taaggtgatt
     5701 aatatgagct ttggtaatgc tgcactagct tacgccaacc ttccagacga aaccaaaaaa
     5761 gcttttgatt atgccaaatc aaaaggtgtt agcattgtga cctcagctgg taatgatagt
     5821 agttttgggg gcaaaacccg tctacctcta gcagatcatc ctgattatgg ggtggttggg
     5881 acacctgcag cggcagattc aacattgaca gttgcttctt acagcccaga taaacagctc
     5941 actgaaactg ctacggtcaa aacagacgat catcaagata aagaaatgcc tgttctttca
     6001 acaaaccgtt ttgagccaaa caaggcttac gactatgctt atgctaatcg tggtacgaaa
     6061 gaggatgatt ttaaggatgt cgaaggtaag attgccctta ttgaacgtgg cgatattgat
     6121 ttcaaagata agattgcaaa cgctaaaaaa gctggtgctg taggggtctt gatctatgac
     6181 aatcaggaca agggcttccc gattgaattg ccaaatgttg atcagatgcc tgcggccttt
     6241 atcagtcgaa aagacggtct cttattaaaa gacaatcccc aaaaaaccat caccttcaat
     6301 gcgacaccta aggtattgcc aacagcaagt ggcaccaaac taagccgctt ctcaagctgg
     6361 ggtctgacag ctgacggcaa tattaagcca gatattgcag cacccggcca agatattttg
     6421 tcgtcagtgg ctaacaacaa gtatgccaaa ctttctggaa ctagtatgtc tgcgccattg
     6481 gtagcgggta tcatgggact attgcaaaag caatatgaga cacagtatcc tgatatgaca
     6541 ccatcagagc gtcttgattt agctaaaaaa gtattgatga gctcagcaac tgccttatat
     6601 gatgaagatg aaaaagctta tttttctcct cgccaacaag gagcaggagc agtcgatgct
     6661 aaaaaagctt cagcagcaac gatgtatgtg acagataagg ataatacctc aagcaaggtt
     6721 cacctgaaca atgtttctga taaatttgaa gtaacagtaa cagttcacaa caaatctgat
     6781 aaacctcaag agttgtatta ccaagcaact gttcaaacag ataaagtaga tggaaaacac
     6841 tttgccttgg ctcctaaagc attgtatgag acatcatggc aaaaaatcac aattccagcc
     6901 aatagcagca aacaagtcac cgttccaatc gatgctagtc gatttagcaa ggacttgctt
     6961 gcccaaatga aaaatggcta tttcttagaa ggttttgttc gtttcaaaca agatcctaaa
     7021 aaagaagagc ttatgagcat tccatatatt ggtttccgag gtgattttgg caatctgtca
     7081 gccttagaaa aaccaatcta tgatagcaaa gacggtagca gctactatca tgaagcaaat
     7141 agtgatgcca aagaccaatt agatggtgat ggattacagt tttacgctct gaaaaacaac
     7201 tttacagcac ttaccacaga gtctaaccca tggacgatta ttaaagctgt caaagaaggg
     7261 gttgaaaaca tagaggatat cgaatcttca gagatcacag aaaccatttt tgcaggtact
     7321 tttgcaaaac aagacgatga tagccactac tatatccacc gtcacgctaa tggcaagcca
     7381 tatgctgcga tctctccaaa tggggacggt aacagagatt atgtccaatt ccaaggtact
     7441 ttcttgcgta atgctaaaaa ccttgtggct gaagtcttgg acaaagaagg aaatgttgtt
     7501 tggacaagtg aggtaaccga gcaagttgtt aaaaactaca acaatgactt ggcaagcaca
     7561 cttggttcaa cccgttttga aaaaacgcgt tgggacggta aagataaaga cggcaaagtt
     7621 gttgctaacg gaacctacac ctatcgtgtt cgctacactc cgattagctc aggtgcaaaa
     7681 gaacaacaca ctgattttga tgtgattgta gacaatacga cacctgaagt cgcaacatcg
     7741 gcaacattct caacagaaga tcgtcgtttg acacttgcat ctaaaccaaa aaccagccaa
     7801 ccgatttacc gtgagcgtat tgcttacact tatatggatg aggatctgcc aacaacagag
     7861 tatatttctc caaatgaaga tggtaccttt actcttcctg aagaggctga aacaatggaa
     7921 ggcggtactg ttccattgaa aatgtcagac tttacttatg ttgttgaaga tatggctggt
     7981 aacatcactt atacaccagt gactaagcta ttggagggtc actctaataa gccagaacaa
     8041 gatggttcag atcaagcacc agacaaaaaa ccagaaacta aaccagaaca agacggttca
     8101 ggtcaaacac caaataaaaa accagaaact aaaccagaaa aagatagttc aggtcaaaca
     8161 ccaggtaaaa ctcctcaaaa aggtcaacct tctcgtgctc tagagaaacg atcttctaag
     8221 cgtgctttag ctacaaaagc atcaacaaga gatcagttac caacgactaa tgacaaggat
     8281 acaaatcgtt tacatctcct taagttagtt atgaccactt tcttcttggg attagtagct
     8341 catatcttta aaacaaaacg cactgaagat tagtacaact gatcagatgg ctactattga
     8401 tgatgagtta gatcagtctt cttttcaaaa gaaggcatag cagtataaaa atagatatgt
     8461 gaggacaata tgcgtagagc agaaaataac aaacacagcc gctattccat tcgcaaactg
     8521 agcgttgggg taacgagtat agcaattgcg agtctctttt tgggaaaagt tgcctatgcc
     8581 gtagatagca tgcagccaat ctcttcgact caaggaaaca gagccaatac atccaaaaaa
     8641 tggtatgata ttaatgagta tggtcttatt cctgtagata taagctttga agctgccaaa
     8701 aaagatttta aagaagaagt agaaaaatca cttttgtctg aagaacaaca gaaaacgtat
     8761 aatcaaaaaa ttgaagctgc aagaaacaaa gacgacctat tgttaacgta tcatagtgag
     8821 tactctaaag atattaagaa acactcaacg tctactgagc cagttgaggc atctgtgcag
     8881 gagacacagg catcagtttc agattcgatg gtgacagatg attcaacatc agttacggct
     8941 gattctcctg agaaaacacc atctacagaa acaccggctc catcaattcc agaggtccca
     9001 gaagccccag cttcatcacc tgagagtgag gagtcttcag tagcatcttc tgaggaaacc
     9061 tcatctccag aaactccagt ggccccagaa gccccagctt tatctgaggc tccagctcaa
     9121 ccagctgaga gtgaggagtc ttcagtagca gctccttctg aggaaacccc atctccagaa
     9181 actccagcgg ccccagaaac tcctgaagag ccagcagctc catctccatc acctgagagt
     9241 gaggaacctt cagtagcagc ttcttctgag gaaaccccat ctccagaaac tccagcggct
     9301 ccagaaactc ctgaagaacc agcagctcca gctcaaccag ctgagagtga agaatcttca
     9361 gtagcagcta cgacaagccc gtctccatca actccagccg aatcagagac tcagacgcca
     9421 ccagctgtta ctaaagactc tgataagcca tcttcagcag ctgaaaaacc agcagcctct
     9481 tcacttgttt cagaacaaac cgttcaacaa ccaacttcaa agagatcttc tgataaaaaa
     9541 gaagagcaag aacagtctta ctctccaaat cgctcattgt caagacaggt tagggcccat
     9601 gagtcaggta agtacttgcc ttcaacaggt gaaaaagcac agccactctt tatagctact
     9661 atgactttga tgtctctatt tggcagtctt ttagtcacaa aacgccaaaa agaaactaaa
     9721 aaatagtatt acttaaactg aatagtatta cttaaactga taagagctta tttaatagag
     9781 agaaccaatt tggttctctc tattttttta gatgtgctgc ctcatcaaaa agaagttagg
     9841 acaaatagct gtcaacaatt gtcttttgct tatctcttca gttgctgtga ttttatcata
     9901 tattgataaa agatggcacg ccaaacgcat gaagaaactt tttttcagaa aactagattc
     9961 ataatgacaa aatcaatcaa agtagtacac tatagatgag gtgactacga tgattgattt
    10021 tattatttct attgatgatt gcgcagttga attggatagt cgtcaatctt ggaaaatt
//
LOCUS       DATXQK010000049         9417 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.049, whole genome shotgun sequence.
ACCESSION   DATXQK010000049 DATXQK010000000
VERSION     DATXQK010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9417
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.049"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            9..197
                     /locus_tag="VC394_001442"
     CDS             9..197
                     /locus_tag="VC394_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Paratox"
                     /protein_id="HEP5994900.1"
                     /translation="MLTYDEFKQAIDNGYITGDTVIIVRKNGQIFDYVLPGEKVRPWE
                     VVTEEVVEEVMVELDYIK"
     gene            complement(349..696)
                     /gene="ssrA"
                     /locus_tag="VC394_001443"
     tmRNA           complement(349..696)
                     /gene="ssrA"
                     /locus_tag="VC394_001443"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            788..1402
                     /locus_tag="VC394_001444"
     CDS             788..1402
                     /locus_tag="VC394_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEP5994901.1"
                     /translation="MTKRNSKAYILWQKIIKILGIIALIGTFFLAFWLYRLGILNDSN
                     ALKDLVQRYRLWGPFVFIVVQIIQIVFPVIPGGLTTVAGFLIFGPVTGFIYNYVGIII
                     GSIVLFLLVKTYGRKFILLFVNDKTFYKYERRLETPGYEKLFIFCMASPVSPADIMVM
                     ITGLTDMSLKRFVTILLITKPISIIGYSYLFIFGKDVISWFLQH"
     gene            complement(1529..2314)
                     /locus_tag="VC394_001445"
     CDS             complement(1529..2314)
                     /locus_tag="VC394_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP5994902.1"
                     /translation="MFGKLLKYEFRSIGKWYFALNAFVIAIAAILSFTIKLFAQSNSD
                     GLFGVLTNKMLPLTLGLTFGSLIAGSLLSTLLIIIKRFSKSVFGREGYLTLTLPVNSH
                     QIILSKLLASFICSVFNTIILAFAIAIVIVPMFNINELLEGFFNSFKMDYFINMLTVL
                     AYVLLSTFTSILLIYLSISIGQLFSNRRGLMAFIAYFILVILISVAATYVHSHIFNIN
                     TSADSFPFTEQKTIYLLILEQFIEMIMFYLATNFIIKNKLNLQ"
     gene            complement(2324..3022)
                     /locus_tag="VC394_001446"
     CDS             complement(2324..3022)
                     /locus_tag="VC394_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5994903.1"
                     /translation="MAHLLQLHHVSKSYREKKAIDDLTITIPNGKIIGLLGPNGSGKT
                     TLIKLINGLLQPNKGEIVIDGYRPCVETKKIISYLPDTTYLNENMRIKDMLEFFSDFY
                     SDFDKSKATSLLRDLELDPEDRFKTLSKGNKEKVQLILVMSRKARLYVLDEPIGGVDP
                     AARDYILKTIINSYCENASVIISTHLISDIEPILDEVIFLKQGRLFLSGNADDLRQEY
                     QQSIDSLFRETYKV"
     gene            complement(3022..3393)
                     /locus_tag="VC394_001447"
     CDS             complement(3022..3393)
                     /locus_tag="VC394_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEP5994904.1"
                     /translation="MSWKFDEKSPIYAQIAQHVMMQIISQEIKSGDQLPTVREYAEIA
                     GVNPNTMQRAFTELEREGMVYSQRTAGRFVTDDQKLIARKRRELAISELESFITNMTK
                     MGFSHTEIIPVLTSFLKEDSQ"
     gene            3578..6688
                     /locus_tag="VC394_001448"
     CDS             3578..6688
                     /locus_tag="VC394_001448"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEP5994905.1"
                     /translation="MFAQLDTKTVYSFMDSLIDLNHYFERAKQFGYHTIGIMDKDNLY
                     GAYHFIKGCQKNGLQPVLGLEVEILYQERQVLLNLIAQNTQGYHQLLKISTAKMSGKL
                     HMDYLCQHLEGIAVIIPSKGWSDTLVVPFDYYIGVDQYTDLSHMDSKRQLIPLRTVRY
                     FAQDDMETLHMLHAIRDNLSLAETPVVESDQELADCQQLTTFYQTHCPQALQNLEDLV
                     SGIYYDFDTNLKLPHFNRDKSAKQELQDLTEAGLKEKGLWKEPYQSRLLRELVIISDM
                     GFDDYFLIVWDLLRFGRSKGYYMGMGRGSAAGSLVAYALNITGIDPVQHDLLFERFLN
                     KERYSMPDIDIDLPDIYRSEFLRYVRNRYGSDHSAQIVTFSTFGPKQAIRDVFKRFGV
                     PEYELTNLTKKIGFKDSLATVYEKSISFRQVINSRTEFQKAFAIAKRIEGNPRQTSIH
                     AAGIVMSDDTLTNHIPLKSGDDMMITQYDAHAVEANGLLKMDFLGLRNLTFVQKMQEK
                     VAKDYGCQIDIAAIDLEDPQTLALFAKGDTKGIFQFEQNGAINLLKRIKPQRFEEIVA
                     TTSLNRPGASDYTTNFIKRREGQEKIDLIDPVIAPILEPTYGIMLYQEQVMQIAQIYA
                     GFTLGKADLLRRAMSKKNLQEMQKMEEDFIASAKHLGRAEETARGLFKRMEKFAGYGF
                     NRSHAFAYSALAFQLAYFKAHYPAVFYDIMMNYSSSNYITDALESDFQVAQVTINSIP
                     YTDKIEASKIYMGLKNIKGLPRDFAYWIVEQRPFNSVEDFLTRTPEKYQKKVFLEPLI
                     KIGLFDCFEPNRKKIMDNLDGLLVFVNELGSLFSDSSFSWVDTKDYSATEKYSLEQEI
                     VGVGMSKHPLIDIAEKSTQTFTPISQLVKESEAVVLIQIDSIRIIRTKTSGQQMAFLS
                     VNDTKKKLDVTLFPQEYAIYKDQLKEGEFYYLKGRIKERDHRLQMVCQQVQMAISQKY
                     WLLVENHQFDSQISEILGAFPGTTPVVIHYQKNKETIALTKIQVHVTENLKEKLRPFV
                     LKTVFR"
     gene            6768..7781
                     /gene="pfkA"
                     /locus_tag="VC394_001449"
     CDS             6768..7781
                     /gene="pfkA"
                     /locus_tag="VC394_001449"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEP5994906.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRKAISEGMEVYGINRGYAGM
                     VDGDIFPLGSKEVGDKISRGGTFLYSARYPEFAQLEGQLAGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAVGIPGTIDNDIAGTDYTIGFDTAVNTAVEAIDKLRDTSSSH
                     GRTFVVEVMGRNAGDIALWAGIASGADQIIVPEEEFDIEKVASTIQYDFEHKGKNHHI
                     IVLAEGVMSGEAFAQKLKEAGDKSDLRVTNLGHILRGGSPTARDRVIASWMGSHAVEL
                     LKEGKGGLAVGIHNEELVESPILGTAEEGALFSLTEEGKIIVNNPHKARLDFAALNRS
                     LSQ"
     gene            7844..9346
                     /gene="pyk"
                     /locus_tag="VC394_001450"
     CDS             7844..9346
                     /gene="pyk"
                     /locus_tag="VC394_001450"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEP5994907.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKYGEDGYWAGQLDVEESAKKIAE
                     LIEAGANVFRFNFSHGDHKEQGDRMATVRLAEEIARQKVGFLLDTKGPEMRTELFADD
                     AKEFSYVTGEKIRVATTQGIQSTRDVIALNVAGSLDIYDEVEVGHTILIDDGKLGLKV
                     IDKDIATRQFIVEVENDGIIAKQKGVNIPNTKIPFPALAERDNADIRFGLEQGLNFIA
                     ISFVRTAKDVEEVREICCETGNDHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVFQKMIITKVNAAGKAVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPVESVRTMATIDRNAQTLLNEYGRLDSSAFPRTNKTDVIASAVK
                     DATHSMDIKLVVTITETGNTARAISKFRPDADILAVTFDEKVQRALMINWGVIPVLAE
                     KPASTDDMFEVAERVAVEAGLVQSGDNIVIVAGVPVGTGGTNTMRVRTVK"
BASE COUNT         2983 a         1569 c         1927 g         2938 t
ORIGIN      
        1 ggtgctttat gctaacatac gacgaattta agcaagcaat tgacaacgga tatatcacag
       61 gagacacagt aataatcgtg cgcaaaaacg gacagatttt tgattatgtg ttgcctggtg
      121 agaaagtcag accatgggag gttgtgaccg aggaggtagt ggaagaagtg atggtggaat
      181 tagactatat caaatgaaga aaactttgag tagacagaaa aataattgaa gagttgtcaa
      241 tttagttgtc aataatatag aaagttatgg taactcatga acaagacaaa aagaaaaaac
      301 cttgatgtaa caaggtttta gtaagttatg attacttacg gtaagcattg atggagctgg
      361 tgggagtcga acccacgtcc aaacacctgc caacatattt gtctacgtcc ataggttatg
      421 tcttcattta acaaactaaa gacacataac tcaagccatt agtttgcgag tctatcaatc
      481 tcttgtcacg aaactagaca atttcgtaac cgtagcttgc tattttgaga cttctaacag
      541 gacacaagca atcttgtaga agtcacgcta gctggttttt aggcagctaa agcgtaagag
      601 tttgtatttt ttgcagttat atttaactgg caatttacat ctgccgatgg gacgcaaaac
      661 atgcctcata atgcctgtcg aatccgtaac aaccccaaat agataattaa ccaatttctt
      721 acactaacta ttctatcaaa acttttttta aatagcaaaa ttttacagct tatggtatga
      781 ttagtctatg acaaaacgta attcaaaagc ctatatatta tggcaaaaaa ttatcaaaat
      841 tttaggaatt attgcactaa ttggtacttt tttcctcgct ttttggctgt atagactagg
      901 tatattaaac gatagtaatg ctcttaaaga cttagtgcag cgctatagat tatgggggcc
      961 gtttgttttt attgtcgtgc agatcataca gattgttttt cctgtgattc ctggaggcct
     1021 tacgacagtt gcaggttttt taatctttgg ccctgtaaca ggttttattt ataattatgt
     1081 aggcatcatt attggcagta tagtcctctt cttgcttgtt aagacttatg gtcgtaaatt
     1141 tatcttactc tttgtaaatg acaaaacatt ttacaagtat gaaaggcgtt tggagacacc
     1201 aggatatgaa aaattattta ttttttgtat ggcatcgcct gtttctccag ctgatattat
     1261 ggttatgatt acgggattga cggacatgtc tttaaagcgc tttgtaacta ttcttttaat
     1321 aactaaacca atttctatta tcggctacag ttatttattt atttttggaa aagatgttat
     1381 atcttggttc ttacagcatt aaggtttggt gttaatttga taaaccgctt ctatttgatg
     1441 gaagacggtt tttttattta ctatgaacac tgaaaaagac tttcttttaa actatagtgt
     1501 gtcatagttt aaaagaaagt cttaacgctt actgtaggtt gagtttattt ttgataataa
     1561 aattagtggc gaggtaaaac attatcattt caataaattg ttccaaaata agaagataaa
     1621 ttgttttttg ctcagtaaat gggaaactat cggcacttgt attaatatta aagatgtgac
     1681 tgtgaacata tgttgcagca acgctaatca gaataactaa tataaaatat gcaataaagg
     1741 ccatcaagcc tcgccgatta gaaaaaagtt gacctataga aatggaaaga taaattaata
     1801 agatactcgt aaatgttgat aataggacat aggctagtac agttagcata ttgatgaaat
     1861 aatccatctt aaaactatta aagaatcctt ctaatagttc gttgatgtta aacattggta
     1921 caattacaat agcgatagca aatgcaagga tgatagtatt gaaaacactg caaataaaag
     1981 aagctagtag ttttgataaa ataatttgat gcgaattaac gggtaaagtc aacgttaagt
     2041 atccttcccg tccaaaaaca cttttgctaa aacgtttgat aatgataagt aatgttgata
     2101 agagggaacc agcgatcagg gagccaaaag ttaaacctaa tgtcagaggt aacatcttat
     2161 tggttagtac tccaaatagt ccatcactat tactttgagc aaacagtttt atcgtaaatg
     2221 acaaaatggc agcgatggca ataacgaaag cattaagcgc aaagtaccat tttccaatag
     2281 atctaaattc gtattttaat aattttccaa acatgatatc ctcctaaacc ttataagttt
     2341 ccctaaagag ggaatcaatg gattgctggt attcttgtct tagatcatca gcattgccag
     2401 ataagaaaag tcttccttgt ttcaagaaaa tgacttcgtc aaggataggt tcaatgtccg
     2461 aaatcaagtg ggttgagata ataacagaag cattttcaca ataactatta atgatggttt
     2521 ttaggatgta gtctcttgcg gcagggtcaa ctccaccaat aggttcatca agtacataga
     2581 gtctggcttt acgactcatc acaagaatta attgtacttt ttctttgttt cctttggata
     2641 gagttttgaa gcggtcttca gggtctagtt ctaaatctct tagaaggctt gttgctttgg
     2701 atttgtcaaa gtcgctgtaa aaatcactaa agaactccaa catgtcctta attctcatat
     2761 tttcattgag ataagtagta tctggtaaat aagaaataat tttttttgtc tcaacacagg
     2821 gacggtagcc atcaatgaca atttctcctt tatttggttg taaaaggcca ttaatgagtt
     2881 taattaaagt tgttttacca cttccatttg gacctaataa tccgataatt ttaccgttcg
     2941 ggatagtaat ggttaagtca tcaatagctt ttttttcacg gtaagatttt gaaacatgat
     3001 gtagttgcaa taggtgtgcc attattgtga atcctccttt aaaaagctcg tcaaaacagg
     3061 gataatctca gtgtgactaa atcccatttt tgtcatattg gtgataaatg attctaattc
     3121 actaattgct aactctcgtc ttttacgagc aattaatttt tgatcgtcag ttacaaaacg
     3181 gccagcagta cgttgcgaat aaaccattcc ttcccgttca agttctgtaa acgctcgttg
     3241 catggtgtta ggattaacac ctgcgatttc tgcatattca cgaacagttg gaagttgatc
     3301 accactcttg atttcttggc taataatttg catcatgaca tgttgtgcta tctgcgcata
     3361 aataggagat ttttcatcaa atttccaaga catgtggtct cctttctgag taatatagat
     3421 atttgtacta attaaatggt acagaaaaaa aaagggaatg tcaagtttta tgccattttt
     3481 cactctttga gaagtagacg tagattagag agaaaattga agcaagaaaa cctttttcat
     3541 agtataatag ggaacaagat aaccaaagga ggaacttatg tttgctcaac ttgatactaa
     3601 aactgtatac tcatttatgg atagtttaat tgacttaaat cattattttg aacgagcaaa
     3661 gcaatttggt taccacacca taggaatcat ggataaggat aatctttatg gtgcttacca
     3721 ttttattaaa ggttgtcaaa aaaatggact gcagccagtt ttaggtttgg aagtagagat
     3781 tctctatcaa gagcggcagg tgctccttaa cttaatcgcc cagaatacac aaggctatca
     3841 tcagctttta aaaatttcca cggcaaaaat gtctggcaag cttcatatgg attacctctg
     3901 ccaacatttg gaagggatag cggttattat tcctagtaag ggttggagcg atacattagt
     3961 ggtccctttt gactactata tcggtgttga tcagtatact gatttatctc atatggattc
     4021 taagaggcag cttatacccc taaggacagt tcgttatttt gcgcaagatg atatggaaac
     4081 cttgcacatg ttgcatgcca ttcgagataa cctcagtctg gcagagaccc ctgtggtaga
     4141 aagtgatcaa gagttagcag attgtcaaca actaaccacc ttctatcaaa cacactgccc
     4201 tcaagctcta cagaatttag aagacttagt gtcaggaatc tattatgatt tcgatacaaa
     4261 tttaaaattg ccacatttta atagagataa gtctgccaag caagaattgc aagacttgac
     4321 tgaggctggt ttgaaggaaa aaggattgtg gaaagagcct tatcaatcgc gcttactgcg
     4381 tgaattggtc attatttctg acatgggctt tgatgattat tttttgattg tgtgggattt
     4441 acttcgcttt ggacgcagta aaggctatta tatgggtatg ggacgtggct cggcggcagg
     4501 tagtctagtg gcttatgctc tgaacattac agggattgat ccagttcaac atgatttgct
     4561 atttgagcgc tttttaaaca aagaacgtta tagcatgcct gatattgata tcgatcttcc
     4621 agatatttac cgttcagaat ttctacggta tgtccgaaat cgttatggta gcgaccattc
     4681 ggcgcaaatt gtgacctttt caacctttgg ccctaaacag gctattcgtg atgttttcaa
     4741 acggttcggg gttccagaat acgaactgac taatctcact aaaaaaattg gttttaaaga
     4801 tagcttggct actgtctatg aaaagtcaat ctcttttagg caggttatta atagtagaac
     4861 tgaatttcaa aaggcctttg ctattgccaa gcgtatcgaa ggaaatccaa gacaaacgtc
     4921 cattcacgca gctggtattg tgatgagtga tgataccttg accaatcata ttcctctaaa
     4981 atcgggcgat gacatgatga tcacccagta tgatgctcat gcggtcgaag ctaatggcct
     5041 gttaaaaatg gattttttgg ggttaagaaa tttgaccttt gttcaaaaaa tgcaagagaa
     5101 ggttgctaaa gactacgggt gtcagatcga tattgcagcc attgatttag aagacccgca
     5161 aacgttggca ctttttgcta aaggggatac caagggaatt ttccaatttg aacaaaatgg
     5221 tgctattaat cttttaaaac ggattaagcc acaacgtttt gaagaaattg ttgccactac
     5281 cagtctaaat agaccagggg caagtgacta taccactaat ttcattaaac gaagagaagg
     5341 acaagaaaaa attgatttga ttgatcctgt gattgctccc attttagagc caacttacgg
     5401 tattatgctt tatcaagaac aagttatgca gattgcacag atttatgctg gttttacgtt
     5461 aggcaaggcc gacttgttaa ggcgtgccat gtctaaaaaa aatctacaag aaatgcaaaa
     5521 aatggaagaa gactttattg cttctgctaa gcacctaggg agagctgaag aaacagctag
     5581 aggacttttt aaacggatgg aaaaatttgc aggttatggt tttaaccgca gccatgcctt
     5641 tgcctattca gctttagctt ttcaattggc ttatttcaaa gcccattacc cggctgtttt
     5701 ttacgatatc atgatgaatt attctagcag taactatatc acagatgctc tagaatcaga
     5761 ttttcaagta gcgcaagtta ccattaatag tattccttac actgataaaa ttgaagctag
     5821 caagatttac atggggctga aaaatattaa ggggttgcca agggattttg cttattggat
     5881 tgttgagcaa agaccattta atagcgtaga ggattttctc actagaactc cagaaaaata
     5941 tcaaaaaaag gttttccttg agcctctgat aaaaataggt ctgtttgatt gctttgagcc
     6001 taaccgtaaa aaaattatgg acaatttgga tggtttactg gtatttgtta atgagcttgg
     6061 ttctcttttt tcagattcgt cctttagttg ggtagatacg aaagattact cagcaactga
     6121 aaaatattct ttggaacagg agatcgttgg ggttggcatg agcaagcatc ctttaattga
     6181 tattgctgag aaaagtaccc aaacttttac tcccatttca cagttagtca aagaaagcga
     6241 agcagtcgta ctgattcaaa tagatagcat taggatcatt agaaccaaaa caagtgggca
     6301 gcaaatggct tttttaagtg tgaatgacac taagaaaaag ctcgatgtca cactttttcc
     6361 acaagagtat gctatttata aagaccaatt aaaagaagga gaattctatt acttaaaagg
     6421 tagaataaaa gaaagagacc atcgactgca gatggtgtgt cagcaagtgc aaatggctat
     6481 tagtcaaaaa tattggttat tagttgaaaa ccatcagttt gattcccaaa tttctgagat
     6541 tttaggtgcc tttccaggaa cgactccagt tgttattcac tatcaaaaaa ataaggaaac
     6601 aattgcatta actaagattc aggttcatgt aacagagaat ttaaaggaaa aacttcgtcc
     6661 ttttgttctg aaaacggttt ttcgataaaa atggacaatt agtgaagtat tttgcaaatg
     6721 aatgtgctat aataagaccg ttaaattaat taaaggagta ccagaaaatg aaacgtattg
     6781 ctgttttaac cagtggcggc gacgctcctg gtatgaacgc tgctattcgt gcagtcgttc
     6841 gtaaagcaat ttcagaagga atggaagttt atggtatcaa ccgtggctat gcaggtatgg
     6901 ttgatggcga cattttccca ctgggttcaa aagaagttgg tgataaaatc tcacgtggtg
     6961 gaactttctt atactcagct cgttatccag agtttgctca acttgaaggt caattggctg
     7021 gtattgagca acttaaaaaa catggtattg aaggtgttgt tgttattggt ggggatggtt
     7081 cttatcatgg cgctatgcgc ttgacagagc acggcttccc agcggtaggt atcccaggaa
     7141 caatcgataa cgatattgcg ggaactgact acactatcgg atttgataca gcagttaaca
     7201 cagctgttga agctattgat aaacttcgtg acacctcatc aagtcacgga cgtacttttg
     7261 tggttgaagt gatgggtcgc aatgcgggtg atattgctct ttgggcaggt attgctagtg
     7321 gtgctgatca aatcattgtt ccagaagaag agtttgatat tgaaaaagtg gcatcaacaa
     7381 tccaatatga ttttgaacac aaaggtaaaa atcaccacat tatcgtctta gctgaaggtg
     7441 ttatgagcgg tgaagctttt gctcaaaaac tgaaagaagc tggcgataag agtgaccttc
     7501 gtgtgacaaa tcttggccac attcttcgtg ggggttcacc aactgctcgt gaccgtgtca
     7561 ttgcttcttg gatgggttca catgctgttg agctacttaa agaaggcaaa ggtggtttgg
     7621 ctgttggtat tcacaatgaa gaacttgttg aaagcccaat ccttggtact gcagaagaag
     7681 gtgctttgtt cagcttgact gaagaaggca agattattgt taataatcca cacaaagctc
     7741 gtttagactt tgcagctttg aaccgttcat tgtcacaata agtttagtta aaattgttaa
     7801 atcgtcaatt attgacaaaa aaataaggga gtttcagagt aaaatgaata aacgcgtaaa
     7861 aatcgttgca acacttggtc ctgcggttga aatccgtggt ggtaaaaaat acggagaaga
     7921 tggctactgg gctggtcaac ttgatgttga agaatctgca aaaaaaatcg ctgaattaat
     7981 tgaagctggt gcgaatgttt tccgtttcaa tttctcacat ggtgaccaca aagaacaagg
     8041 tgaccgtatg gcaactgtcc gccttgcaga agaaattgct cgtcaaaaag ttggtttcct
     8101 tttggatact aaaggtcctg aaatgcgtac ggaattgttt gcagatgatg ctaaagagtt
     8161 cagctatgtt actggagaaa aaatccgtgt agcgactaca caaggtattc aatcaactcg
     8221 tgatgttatt gctttgaacg ttgctggtag ccttgacatc tacgatgaag tagaagtagg
     8281 tcatactatt cttatcgatg atggtaaact tggtttgaaa gtgatcgaca aagatatcgc
     8341 aacacgtcaa tttatcgttg aagtagaaaa cgacggtatt attgctaaac aaaaaggtgt
     8401 taatatccct aacactaaaa ttcctttccc agcacttgcg gaacgcgaca atgctgatat
     8461 ccgttttggt cttgaacaag gacttaactt tattgcaatc tcatttgttc gtacagccaa
     8521 agacgttgag gaagttcgtg aaatctgttg cgaaactggt aacgaccatg tgcaattatt
     8581 tgctaagatt gaaaaccaac aaggtatcga taatcttgat gaaatcatcg aagctgctga
     8641 tggtatcatg attgcacgtg gcgacatggg tatcgaagta ccatttgaaa tggttccagt
     8701 tttccaaaaa atgattatca ctaaagtgaa tgcagctggt aaagcagtta tcacagcaac
     8761 aaacatgctt gaaacaatga ctgaaaaacc acgtgcgaca cgttctgaag tatctgacgt
     8821 cttcaatgct gttatcgatg gtactgatgc aacaatgctt tcaggagagt cagctaacgg
     8881 taaatacccg gttgaatctg ttcgtacaat ggcgactatt gatcgtaatg cacaaactct
     8941 tcttaacgaa tatggtcgtt tagattcatc tgcctttcca cgtaccaata aaacagatgt
     9001 tattgcttca gctgttaaag atgccactca ttcaatggac atcaaacttg ttgtgactat
     9061 tactgagaca ggtaatacag ctcgtgcgat ttctaaattc cgtccagatg cggacatctt
     9121 agctgtaaca tttgatgaaa aagtacaacg tgctttgatg attaactggg gtgttattcc
     9181 tgttcttgct gaaaaacctg catctactga tgacatgttt gaagtagcag agcgtgttgc
     9241 tgtggaggca ggcttagttc aatctgggga taatattgtt attgttgctg gtgttccagt
     9301 tggtactggt ggaacgaata ctatgcgtgt tcgtacagta aaataaatgt gttataacta
     9361 tttacctaaa ggttagaagg caagcttcta accttttttg atatcaaaaa tatcatg
//
LOCUS       DATXQK010000050         9333 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.050, whole genome shotgun sequence.
ACCESSION   DATXQK010000050 DATXQK010000000
VERSION     DATXQK010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9333
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.050"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            96..530
                     /locus_tag="VC394_001451"
     CDS             96..530
                     /locus_tag="VC394_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP5994908.1"
                     /translation="MIDVSKKLKQLRIDRGIAKSRLITYLSITLRTYDRYEDGSRTPD
                     LVTLMKLSDFYQCSLDELVKGNTNDTLVDRALIVSYRDLKDKGLNDKLAHGLVKQVYD
                     TYEFEKIPQISDANRIKFIYKEDLVTLLESIVSKIQEEKKCD"
     gene            688..1128
                     /locus_tag="VC394_001452"
     CDS             688..1128
                     /locus_tag="VC394_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEP5994909.1"
                     /translation="MEHRLSMHYFQVKKEIFGVLLLSPVILLIMWGYVSSNQDDGRLT
                     QKQYYETVVNQTVNLVFYKKSCPYCKVAKKEIATQAKDSQVITYYVDTSTGEGQKLVK
                     KYHVKYAPTIVSIRKGNIQSFLYARDRGNNIIVEKEKIKEVFSQ"
     gene            1252..1644
                     /locus_tag="VC394_001453"
     CDS             1252..1644
                     /locus_tag="VC394_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994910.1"
                     /translation="MLNNLFIEKKGIMMSRKGVQEYDVTNVSERSIKIIKKAMYDEGT
                     GFKPIHFYGIAICEGTREFYRPTYPFVRSEEDLDNLKDFINLYETDLLTFYTHGHNYD
                     FGCFIYGIGNDGKDKFRDRWFKEGVIFY"
     gene            1737..3977
                     /locus_tag="VC394_001454"
     CDS             1737..3977
                     /locus_tag="VC394_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018366738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEP5994911.1"
                     /translation="MKQTKVLTGIAVAALSTGTGLVQADDLSNTRPAKAAEVVTHTEV
                     TENQVADAKTKADEATKAVKDQEATVKAIEAEKSQAQQSLVEATTAVNDTEKLASEAT
                     PDGLTKAQEETEASQSAVTDAQAQLEAAQEAESKAQTAVDHQKETVAKASQAVNQAKA
                     ALTTSNQAADVSAAEEKVLEAQEAVTKAETQVSQAQEADAKHESDVRHAKEELDLKSQ
                     KLTEKQTTLEQVMAAIEAERLTKPVENGTYFNQRDNEWQKAYGGKTFAATGCVPSALA
                     MVFSELAKREVTPTEIADYLWHNTDEFNKHYGGTSGKGLVAATNHYGFASTHLDSQST
                     IIAALQAGHHVLAAVQNNKFSPWGSQYSHEIVLRGYSNGNTYVYDPYNRANIGWYPIA
                     NLWNEQSRDAIDTAEVGVPFFKITTQKMAQLEMAKAVANEGLDEAKQAVKQAEANVTA
                     LEQVISQTSQAKEQLAAAKANLVTAKDMLDKAKQTAKLTEQDLETKTANLKQAQEHLT
                     LKQDDLAKAQTELAAQEKSLEQLTQVLSLAQENLGRATKAVEQTKADLTQKQAYVTNL
                     KQVTKLRQESQAKVNQAKAVLSEASARLEQELETLKTLQAKEANLTAQYQAIFKAYQA
                     VLAVKHHSQLAESKAKLEAKGEEAVPVVNEVGHVTDYVSGPTQLADAVAVRTEETSSK
                     TKNTSPVSVKLMAKQGHQKALETIVTSQSSLPQTGEQVVSILSLVGLNFLGLSSFLGL
                     RKQEEK"
     gene            4147..8205
                     /locus_tag="VC394_001455"
     CDS             4147..8205
                     /locus_tag="VC394_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEP5994912.1"
                     /translation="MKQMETKGYGYFRKTKAYGLVCGITLAGALTLGTTSVSADDVTT
                     LNPATNLTTLQTPPTADQTQLAHQAGQQSGELVSEVSNTEWDNAVTTAQKAGVTVKQS
                     EKVTHDSLSSAQADLEKQTQAVTEATTKQEANTTAINQAVSENKAIDQANRDEKARVD
                     ALNTKGEADTKAKNETGQAQVDAQNKQAQAAADAMNAKLKADYEAKLTEIKQIESENE
                     AIRQRNQQASQATHQANQAAQAAYQEKLAEIERIKAENAAIRDRNAKAQQEAERQNQA
                     LQAAYEAKLAEIKQIESENAAIRQRNEQAGQATNQTNQAAQAAYQEKLAEIERIKAGN
                     AAIRDRNAKAQQEAERQNQALQAAYEAKLAEIKQIESENAAIRKRNEQAGQTTNQTNQ
                     AAQAAYQEKLAEIERINAENAAIRDRNTKARQEAERQNQQLKTTYEAQLSAYQQALQA
                     KKEAENKAIDQVVFGIDAKANGVDNAEYGNSIMTVTTQPDGSFVFKHDMIDGVKTIGH
                     GTLTGKINHHYEANKDGSITAYIDSVTFDKYEYQNVAKNDAVDKNIAFRILSATGQEL
                     FVKAHDGDKTFSETLNKTVSLKLTYQLKPHEAVKDIKVFQLHDDWVHDTHGSALVSYV
                     NNNDAVPHIEVPEKPVEPDMVTPKVEQEKPVPEAPQKPTDGVPNLEKEKPVPPTPVKP
                     EAVKPVLEQEKPVPEAPQKPTDDVPNLEKEKPVPPTPVKPEAVKPVLEQEKPVPEAPQ
                     KPTDDVPNLEKEKPVPPTPVEPEAIKPDLQSFTPEVYDPIKPVVKPHVTVPEKVVYEV
                     MVHPVQVKQTPTNVKSVTNSDQVNIDGQLVPKGSTVTWELVNTSLKAGRQDITSYELT
                     DPLPDGFELDVTATQTLSPEWVITTDEAGKVSLKASQSLLAHFNAKRDQDVEVPKVSL
                     VGRLLNDAGTYHNTFKTVVTTPTGSYTVISNTPVIYTPGNDPKTPRNPGGDNPTPHDN
                     LIQPTKTIVDDKGQSIDGKSVLPNSTLTYVAKQDFDQYKGMTAAKESVMKGFIYVDDY
                     KDEAIDGHSLVVNSIKAANGDDVTNLLEMRHVLSQDTLDDKLKALIKASGISPVGEFY
                     MWVAKDPAAFYKAYVQKGLDITYNLSFKLKQDFKKGDITNQTYQIDFGNGYYGNIVVN
                     HLSELTVHKDVFDKEGGQSINAGTVKVGDEVTYRLEGWVVPTNRGYDLTEYKFVDQLQ
                     HTHDLYQKDKVLATVDITLSDGSVITKGTDLAKYTETVYNKETGHYELAFKQDFLAKV
                     VRSSEFGADAFVVVKRIKAGDVANEYTLYVNGNPVKSNKVTTHTPEQPQPVTPKAPAL
                     PSTGEQGVSILTALGAALLSLLGYVGLKKRQQ"
     gene            8467..8772
                     /locus_tag="VC394_001456"
     CDS             8467..8772
                     /locus_tag="VC394_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HEP5994913.1"
                     /translation="MEKSTLKLKPDFIGKAWYEKLGNAPRRIDGEAGQFQRHIVSNEK
                     HGVFHVITPALAQVFKTDDVVEIDGEAIFFEDRALNGNSVAPALNVRATGLKLAGGK"
     gene            8775..9230
                     /locus_tag="VC394_001457"
     CDS             8775..9230
                     /locus_tag="VC394_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEP5994914.1"
                     /translation="MAKVGLNFGEKIQIVDKSYRVLNDGLVDLKEIFGDLSFRSFEET
                     DLIYEDDMTQPRRQDGSYPQIPTGESRGLVVGIFSPVQQQTEFVTLVDMSLAEFEALQ
                     LKYREPVELEGIIVTYSSVGSNQFKLFASKIKRSVSQKVANEGNHKQEK"
BASE COUNT         3295 a         1622 c         1970 g         2446 t
ORIGIN      
        1 tctctaagct atattttttt atttttggaa agggtattaa gcatagtatc ttttttaaag
       61 gtaaaaattt agaatgattg aaagggagct gaataatgat tgatgtatca aaaaaactaa
      121 aacaactaag aattgataga gggattgcta aatcgcggtt gatcacttat ttatcgataa
      181 ctttaagaac ttatgacaga tatgaagatg gtagtcgtac tcctgattta gtaacgttaa
      241 tgaaattatc ggatttttat caatgctcgc ttgatgaatt agttaaaggg aacacaaatg
      301 atacgcttgt tgatagggca ttgattgttt cgtatagaga tcttaaagat aagggtttaa
      361 atgataaatt ggctcatggt ctagtgaagc aggtctatga tacttatgaa tttgagaaga
      421 ttcctcaaat atctgatgct aatagaatta agtttatcta taaagaagat ttggttactt
      481 tacttgaaag tatcgtgtca aaaatacaag aggagaagaa atgtgactag ctaaaagatt
      541 ttggaggtaa tgaatgttag tttatgaatt tattaaacaa aagattttgt gtcatcagct
      601 aaccaggttg ttacgaatag gtactgtaat gataactgga ctctcctata gtttagataa
      661 ctggaaaaag tggaggggga ttgaaatatg gagcatcgat tgagtatgca ctattttcaa
      721 gtgaaaaaag aaatttttgg ggtattatta ttgagtcccg ttattttatt gattatgtgg
      781 ggctatgttt ctagcaatca agatgatggt cgactaacgc aaaagcaata ctatgaaaca
      841 gtggtcaacc aaacggttaa tttagtgttt tataaaaagt catgtcccta ttgcaaagta
      901 gctaaaaaag aaattgctac tcaagctaag gatagtcaag ttattactta ttatgttgat
      961 actagtacgg gagaaggaca aaaattggtt aaaaaatacc atgttaagta cgcacctaca
     1021 atagtatcaa ttagaaaagg aaatattcaa tcatttttgt atgctcgaga tagaggcaac
     1081 aacattatag tagaaaaaga aaagataaaa gaagtattta gtcagtaagc tatgacttta
     1141 tgtgtcatag cttttttata tttgttgaaa acaagggtaa aatacagtaa atctccattc
     1201 ttcattctcc atttttaaac cgaaaagatt ttactcttgt tttgagcaag tatgcttaat
     1261 aatttattta tagaaaagaa aggtattatg atgtctagga aaggagtaca agaatacgat
     1321 gtaacaaatg tatctgaaag aagtataaag ataattaaga aagctatgta tgatgaagga
     1381 acaggtttta agccaattca tttttatgga atagctatct gcgaagggac tagagaattt
     1441 tatagaccta cttatccatt tgttagaagt gaagaagatt tagataactt aaaagatttt
     1501 attaacttat atgagacaga tttgttaact ttttatacac atggtcataa ttatgacttt
     1561 ggctgtttta tttatggtat aggaaatgac ggaaaagata agttccgtga tagatggttt
     1621 aaagaaggag tcattttcta ttgaatttta aaataattaa aaaaaagatg acccaaggta
     1681 acattaaagt agtataataa tcttaataaa ggaagttata ggaagggcaa ctacttatga
     1741 aacaaacaaa agtattaaca ggaatcgcag tcgcagcttt atctacaggt acaggtcttg
     1801 tgcaggcaga tgatttatca aacacaagac cagctaaagc agctgaagtt gtgacacata
     1861 cggaagtgac agaaaatcaa gtggcggacg ctaaaacaaa agctgatgag gcaacgaaag
     1921 ccgttaagga tcaggaagct acggttaagg ctattgaggc agaaaaatct caagcacaac
     1981 aatctttagt tgaggcgaca acagcagtta atgataccga aaagttagct agtgaagcga
     2041 caccagatgg tctaacaaaa gcccaagaag agactgaggc tagtcaaagc gctgttactg
     2101 atgcacaagc acaacttgaa gcagctcaag aggctgaatc aaaagcccaa acagcagttg
     2161 accatcagaa agaaacagtc gccaaagcgt ctcaagctgt taatcaagcc aaagcagctc
     2221 tcacgacaag taatcaagcc gctgatgtta gtgcagcaga agaaaaagtg ttagaggctc
     2281 aagaagcggt tacgaaagca gaaacacaag ttagtcaggc tcaagaagca gatgctaaac
     2341 atgagtctga tgtgagacat gccaaagaag agcttgacct taagtctcaa aaattaacag
     2401 aaaaacaaac aactctagaa caagtcatgg ctgcgattga agcagagcgt ctgactaagc
     2461 cagttgaaaa tggcacttac tttaaccaaa gagataacga gtggcaaaaa gcctatggtg
     2521 ggaagacctt tgcggcgaca ggatgtgtgc ctagtgcttt ggctatggtt ttttcagagc
     2581 tggctaaaag agaggtgaca ccgactgaga ttgctgatta cttatggcat aacacagatg
     2641 agtttaataa acattatggt ggtacaagtg gtaaaggact tgtagctgct actaatcatt
     2701 atggctttgc ttcaacacat ttagatagtc aatcaactat tattgcagct ttacaagcag
     2761 gtcatcatgt tttggctgca gtacagaata ataaatttag tccttgggga tcacaatata
     2821 gtcatgaaat tgtcttgcgt ggttactcaa atggcaatac ttatgtttac gatccttata
     2881 atcgagctaa cattggttgg taccctattg ctaatttatg gaatgaacaa agtcgtgatg
     2941 ctattgatac agcagaggtt ggagtacctt tctttaaaat aacaactcaa aaaatggcac
     3001 aactcgaaat ggcaaaagca gtagctaatg aaggcttaga tgaagctaag caagctgtta
     3061 aacaagcgga agcaaatgtg acagccttag aacaagttat ttctcaaacc tcacaagcca
     3121 aagaacaatt agctgcagct aaggctaact tagtcacagc caaagatatg ctggataagg
     3181 ctaaacaaac ggctaaatta acagaacaag acttagaaac gaaaacagct aatcttaagc
     3241 aagctcaaga acatctaacc cttaaacaag atgacctagc taaagctcaa acggagctag
     3301 cagctcaaga gaaaagcttg gaacagttaa ctcaagtgct ttcattagct caagaaaatc
     3361 ttggccgtgc tacaaaagca gttgaacaga ctaaggctga tcttacccaa aaacaggctt
     3421 atgtgacgaa tcttaagcaa gtgacaaaac tgagacaaga aagtcaagcc aaagtaaacc
     3481 aagctaaggc ggttctcagt gaagcaagtg ctcgtttaga acaagaactt gagaccttaa
     3541 aaaccttaca agccaaagag gctaacctaa cagcacagta tcaagctatc tttaaagcct
     3601 atcaagctgt tttagctgtg aaacaccact cacaattggc tgaaagcaaa gctaagcttg
     3661 aagctaaggg agaagaagct gttcctgtgg tgaatgaagt gggacacgta acggattatg
     3721 tgtctgggcc aacacagttg gctgatgctg tagcagtgag aacagaagaa acatcatcaa
     3781 aaacaaagaa cacttctcct gttagtgtga aattgatggc caagcaaggt catcaaaagg
     3841 ctcttgaaac tatcgttact tcacaaagta gccttcctca gacaggtgag caagttgtct
     3901 ctattttgag cttagtaggt ttgaattttt taggcttaag tagttttttg gggcttagga
     3961 agcaagagga aaagtaataa aatatagaac aagaggtcat tctttagaga atggcttttt
     4021 gattggagtt atgatgaaaa tagtttttaa ttggaaggca agattacctt ccttttcttt
     4081 ttagtaaaac atataagacc tgggatcgtc acaaaaagca acctagaaga gagatgagaa
     4141 aataacatga aacaaatgga aactaagggt tatggttatt ttagaaaaac aaaggcctat
     4201 ggtcttgttt gtggtattac tttagcggga gcgttgacgt tgggtacaac aagtgtttct
     4261 gcggatgacg taactacttt aaacccagcg actaacttaa caacgctaca aacacctcca
     4321 acagctgatc aaacacagct agctcatcaa gctggccagc agtcagggga gttggtatca
     4381 gaggtgagca ataccgaatg ggataatgct gtcacaacag ctcaaaaagc aggtgttact
     4441 gtcaaacagt ctgagaaggt cacacatgat agcttatcat ctgcgcaagc cgatttagag
     4501 aaacaaactc aagctgtcac agaagcgaca actaaacaag aagccaacac aacagctatt
     4561 aatcaagcag tttcggaaaa taaagcgatt gatcaagcta atcgtgatga aaaagctaga
     4621 gttgacgctc ttaatactaa aggggaagct gataccaaag ctaaaaatga aactggtcaa
     4681 gcccaagtag atgcacaaaa taaacaagcc caagcagcag ctgatgccat gaatgccaaa
     4741 ttaaaggcag actatgaggc caagttaaca gaaatcaagc agattgaatc agaaaatgaa
     4801 gctatccgtc agcgcaacca acaagctagt caagcgactc atcaagccaa tcaagcagcc
     4861 caagctgcct atcaagaaaa gctagcagag attgagcgca tcaaagccga aaatgctgcc
     4921 attcgcgatc gtaatgctaa ggctcaacaa gaagcagagc gtcaaaacca agcgctccaa
     4981 gcagcttatg aagcaaaatt ggcagaaatc aagcagattg aatcagagaa tgctgctatc
     5041 cgtcagcgta atgaacaagc aggtcaagcg acgaatcaaa ccaatcaagc agctcaagct
     5101 gcctatcaag aaaagctagc agagattgag cgcattaaag ctggaaatgc tgccattcgc
     5161 gatcgtaatg ctaaggctca acaagaagca gagcgtcaaa accaagcgct ccaagcagct
     5221 tatgaagcaa aattggcaga aatcaagcag attgaatcag agaatgctgc tattcgtaag
     5281 cgtaatgaac aagcaggtca aacgacgaat caaacgaatc aagcggctca agctgcctat
     5341 caagaaaagc tagcagagat tgagcgtatt aacgccgaga atgccgccat tcgtgatcgc
     5401 aacactaaag ctcgacaaga agcagaacgt caaaaccaac agttaaaaac tacttatgaa
     5461 gctcaattat cagcctacca acaagcacta caagctaaaa aagaagctga aaacaaagct
     5521 atcgatcagg ttgtctttgg aattgatgca aaagccaacg gtgttgataa tgcagaatat
     5581 ggtaattcaa tcatgactgt tacaacgcaa ccagatggtt cgtttgtttt taagcatgat
     5641 atgattgatg gtgtcaaaac aattggtcat gggactttaa cgggtaaaat taatcatcat
     5701 tatgaggcta ataaagatgg tagcattaca gcttatattg atagtgtaac atttgataag
     5761 tatgaatatc aaaatgtagc taaaaatgac gcagttgata aaaatattgc ttttcgtatt
     5821 ttaagtgcca caggtcaaga attatttgtt aaagcccatg atggtgataa aacttttagt
     5881 gaaactttaa ataagacagt gtctcttaag ttaacttacc aactaaaacc gcatgaagct
     5941 gtaaaagata tcaaagtatt tcaactgcat gatgactggg tacacgacac acatggttct
     6001 gctttagttt cttatgtgaa caacaatgat gctgtgccac atattgaagt accagaaaag
     6061 ccagtggagc ctgatatggt tacaccaaaa gttgagcaag aaaaaccagt cccagaggct
     6121 cctcaaaaac caacagatgg tgttccaaac ttagagaagg aaaagccggt tcctccaacc
     6181 ccagttaaac cagaggcagt taaacctgtt ttagaacaag aaaaaccagt accagaggct
     6241 cctcaaaagc caacagatga tgttccaaac ttagagaagg aaaagccggt accaccaact
     6301 ccagttaaac cagaggcagt taaacctgtt ttagaacaag aaaaaccagt cccagaagct
     6361 cctcaaaagc caacagatga tgttccaaac ttagagaagg aaaaaccagt accaccaacc
     6421 ccagttgaac ctgaagcaat taagccagat ctccaaagct ttacgccaga ggtttatgac
     6481 ccgattaaac cagttgtgaa accccatgtc accgttcctg aaaaggtcgt ctatgaggtt
     6541 atggttcatc ctgttcaagt gaaacaaacg ccaacaaatg ttaagtctgt tactaatagt
     6601 gatcaggtta atatcgatgg acaactggta ccaaaaggat caacggtaac gtgggagctt
     6661 gttaatactt ctctcaaagc tggtcgtcag gacatcacaa gctatgaact aactgatcca
     6721 ctaccagatg gttttgagtt agatgttacc gccacacaaa ccctttctcc tgaatgggtg
     6781 ataacaacgg atgaagcagg caaggttagt cttaaggctt ctcaatccct tttggctcac
     6841 ttcaatgcta aacgtgatca agatgtagaa gtccctaagg tatcactggt tggtcgttta
     6901 ctgaatgatg cggggactta tcacaatacc tttaagacgg tggtcactac tccaactggt
     6961 agctacacgg taatctctaa cacaccagtg atctatacac cagggaacga tccgaaaaca
     7021 cctcgtaacc caggaggaga taatccaaca ccgcatgata atctgattca gccaactaaa
     7081 accattgtgg acgacaaggg tcagtccatt gatgggaagt ctgtcttgcc aaacagcacg
     7141 ctgacttacg tggctaagca agattttgat cagtacaaag ggatgacagc tgccaaagaa
     7201 agtgtcatga aaggctttat ctatgtcgat gattacaagg atgaagcgat agatggtcat
     7261 tcactcgttg tgaattccat taaggccgct aacggagatg atgtaaccaa tctcttagag
     7321 atgcgccatg tgttatctca agatacgctt gacgataagc taaaagcact catcaaggca
     7381 tcaggcattt ctccagtcgg tgaattttac atgtgggtag ctaaagaccc agcagccttt
     7441 tacaaagctt atgttcaaaa agggttagac attacctaca acttgtcctt taaacttaaa
     7501 caggacttta agaaaggtga cattactaac caaacctacc aaattgattt tggaaacgga
     7561 tactacggta atatcgtggt aaaccatctg tctgaactaa ctgttcataa ggatgttttc
     7621 gataaagaag gtggtcaatc aatcaatgct ggcactgtta aagttggtga tgaggtcacc
     7681 tatcgcttgg aaggttgggt tgttccgact aaccgtggct atgacttgac agaatacaaa
     7741 tttgtggatc agcttcagca tacgcatgac ctctaccaga aagacaaggt ccttgcgacg
     7801 gttgatatta ctttatcaga tggtagtgtt atcactaaag gtactgactt agcgaaatat
     7861 actgagacag tttacaataa ggaaacaggt cactatgaat tagcgttcaa gcaagacttc
     7921 ttagctaagg tcgttcgttc tagcgaattt ggtgcagatg cttttgtggt tgttaaacgc
     7981 atcaaagcag gtgatgtggc aaatgagtat accttgtatg tcaacggtaa tcctgtgaag
     8041 tctaataaag tcaccactca tacgccagaa caacctcaac ctgtaactcc taaagcacca
     8101 gctttaccat ccacaggaga gcaaggagta tctatcttaa cggcgcttgg ggccgcttta
     8161 ctctcactct taggttatgt agggcttaaa aagcgtcaac agtaagttat taatggataa
     8221 gccatcaaag tagattactt tgggtctatc cattctctct agcaagtaac tattaggggg
     8281 atctcctttc ccttcaaaaa actagccgaa aaagtttact attggttact tgttagggtg
     8341 agtggataga tcttggttta tcactagaat agtgaccgaa aagtcaaaaa actaagtggt
     8401 aaggctagga aataattgat aagacaatcc tagccttttt attttaaaaa tgaaaagagg
     8461 taaattatgg agaaatcaac attaaagtta aaaccagatt ttattggtaa agcatggtat
     8521 gaaaaattag gaaatgctcc ccgtcggatt gatggcgaag cagggcagtt tcagcgtcat
     8581 attgtgtcga atgaaaagca tggggttttt catgtgatta caccggcttt agcccaagta
     8641 tttaaaacag atgatgtagt tgaaattgat ggtgaagcta tcttttttga agatagagct
     8701 ttgaatggta atagtgtagc acctgcactt aatgtacgtg caacaggatt gaaattagct
     8761 ggaggtaaat agtaatggca aaagtaggtt taaattttgg agagaaaatt cagattgtag
     8821 ataaaagtta tcgcgtatta aatgatggtt tagttgactt gaaagaaatt tttggtgact
     8881 tatcatttcg atcctttgaa gaaacagatt taatttatga agatgatatg actcaaccaa
     8941 gacgtcaaga tggttcttac ccacaaattc ctacaggcga atcacgtgga cttgttgttg
     9001 gtattttctc accagtgcag caacaaacag aatttgttac tttggttgat atgagccttg
     9061 cggagtttga agcgttgcag ttgaaatatc gtgagccagt tgaattagaa ggtattatcg
     9121 tgacttattc ttctgtagga agtaatcagt ttaagttatt tgcttccaaa attaaacgct
     9181 ctgttagcca aaaagtagct aatgaaggta atcataagca agaaaaataa ataataaggg
     9241 agaggtaaga tgcgtttact gcctgaatat agagggatta aggtaaaacc atatatgagg
     9301 tatatgagtt attacctttt ttcttttcta ttt
//
LOCUS       DATXQK010000051         8991 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.051, whole genome shotgun sequence.
ACCESSION   DATXQK010000051 DATXQK010000000
VERSION     DATXQK010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8991
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.051"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            88..1008
                     /gene="lmb"
                     /locus_tag="VC394_001458"
     CDS             88..1008
                     /gene="lmb"
                     /locus_tag="VC394_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/laminin-binding adhesin Lmb"
                     /protein_id="HEP5994915.1"
                     /translation="MKKGFFLMAMVVSLVMIAGCDKSANPKQPTQGMSVVTSFYPMYA
                     MTKEVSGDLNDVRMIQSGAGIHSFEPSVNDVAAIYDADLFVYHSHTLEAWARDLDPNL
                     KKSKVDVFEASKPLTLDRVKGLEDMEVTQGIDPATLYDPHTWTDPVLAGEEAVNIAKE
                     LGRLDPKHKDSYTKKAKAFKKEAEQLTEEYTQKFKKVRSKTFVTQHTAFSYLAKRFGL
                     KQLGISGISPEQEPSPRQLKEIQDFVKEYNVKTIFAEDNVNPKIAHAIAKSTGAKVKT
                     LSPLEAAPSGNKTYLENLRANLEVLYQQLK"
     gene            1021..3498
                     /locus_tag="VC394_001459"
     CDS             1021..3498
                     /locus_tag="VC394_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEP5994916.1"
                     /translation="MKKTYGYIGSVAAILLATHIGSYQLGKHHMGSATKDNQIAYIDD
                     SKGKAKAPKTNKTMDQISAEEGISAEQIVVKITDQGYVTSHGDHYHFYNGKVPYDAII
                     SEELLMTDPNYHFKQSDVINEILDGYVIKVNGNYYVYLKPGSKRKNIRTKQQIAEQVA
                     KGTKEAKEKGLAQVAHLSKEEVAAVNEAKRQGRYTTDDGYIFSPTDIIDDLGDAYLVP
                     HGNHYHYIPKKDLSPSELAAAQAYWSQKQGRGARPSDYRPTPAPAPGRRKAPIPDVTP
                     NPGQGHQPDNGGYHPAPPRPNDASQNKHQRDEFKGKTFKELLDQLHRLDLKYRHVEED
                     GLIFEPTQVIKSNAFGYVVPHGDHYHIIPRSQLSPLEMELADRYLAGQTEDNDSGSDH
                     SKPSDKEVTHTFLGHRIKAYGKGLDGKPYDTSDAYVFSKESIHSVDKSGVTAKHGDHF
                     HYIGFGELEQYELDEVANWVKAKGQADELAAALDQEQGKEKPLFDTKKVSRKVTKDGK
                     VGYMMPKDGKDYFYARDQLDLTQIAFAEQELMLKDKKHYRYDIVDTGIEPRLAVDVSS
                     LPMHAGNATYDTGSSFVIPHIDHIHVVPYSWLTRDQIATIKYVMQHPEVRPDVWSKPG
                     HEESGSVIPNVTPLDKRAGMPNWQIIHSAEEVQKALAEGRFATPDGYIFDPRDVLAKE
                     TFVWKDGSFSIPRADGSSLRTINKSDLSQAEWQQAQELLAKKNAGDATDTDKPKEKQQ
                     ADKSNENQQPSEASKEEEKESDDFIDSLPDYGLDRATLEDHINQLAQKANIDPKYLIF
                     QPEGVQFYNKNGELVTYDIKTLQQINP"
     gene            3667..3867
                     /locus_tag="VC394_001460"
     CDS             3667..3867
                     /locus_tag="VC394_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEP5994917.1"
                     /translation="MSEEKLKSKIEQASGGLKEGAGKLTGDKELEAKGFVEKTIAKGK
                     ELADDAKEAVEGAVDAVKEKLK"
     gene            complement(3949..4575)
                     /locus_tag="VC394_001461"
     CDS             complement(3949..4575)
                     /locus_tag="VC394_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5994918.1"
                     /translation="MTLEAKKLGFYHKKDQWLFKEIDLEVAPGQILGIFGQSGCGKTS
                     LSRVLAGFLQPKSGEVLVDGSHLPNKAFRPVQLIQQHPEQTMNPLWPMKKSLEEAYYP
                     SQDLRDAFGIQEKWLKRRPSELSGGELQRFSIVRSLHPETKYLIADEMTTMLDSITQA
                     SVWKSLLEIVKDRNLGLIIISHEFDMLEKLCDACYMIEENRTQLFKHT"
     gene            complement(4559..5362)
                     /locus_tag="VC394_001462"
     CDS             complement(4559..5362)
                     /locus_tag="VC394_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5994919.1"
                     /translation="MTETLLSIKDLSITFTQYGRFLKPFQSTPIQALNLEVKKGELLA
                     IIGASGSGKSLLAHAIMDILPKNAAVTGDMIYRGQSLTSKRIKQLRGKEMTLIPQSVN
                     YLDPSMKVKHQVRLGISENAKATQEGLFQQFGLKESDGNLYPFQLSGGMLRRVLFTTC
                     ISDTVSLIIADEPTPGLHPDALQMVLDQLRSFADKGISVIFITHDIVAASQIADRITI
                     FKEGKAIETAPASFFSGGGEQLQTEFARRLWRTLPQQDFLKGVTHDLRG"
     gene            complement(5374..6195)
                     /locus_tag="VC394_001463"
     CDS             complement(5374..6195)
                     /locus_tag="VC394_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015631767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP5994920.1"
                     /translation="MILKRRTMVLWQLGIAISLILSILALNLYFYRTPLETNASLRNL
                     APSLNHLFGTDGLGRDMFVRTIKGLYFSLQVGLLGALMGVSLATVFGVLAGLGNSLID
                     KIIAWLVDLFIGMPHLIFMILISFVVGKGAQGVIIATAVTHWPSLARLIRNEVYDLKN
                     KAFVQLSKSMGKTPYYIVRHHILPLIASQIFIGFILLFPHVILHEASMTFLGFGLSAE
                     QPSVGIILSEAAKHISLGNWWLVVFPGLYLILVVNAFDTIGESLKKLFYPQTDHF"
     gene            complement(6192..7169)
                     /locus_tag="VC394_001464"
     CDS             complement(6192..7169)
                     /locus_tag="VC394_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP5994921.1"
                     /translation="MKRTTIIIIWKIIRCVTLIFGVSVLTFVLLKQSPVDPVMASVNY
                     DTSLTPAQYKAIAHHYGLDKPALVQYFIWLKNVIQGHLGTSLVYRQPVSDIIRSRAGA
                     SFILMGLSWILSGLIGFILGTLSAFHQGKLLDRVVRWFSYLQISVPTFWIGLIFLLIF
                     SVQLGWFPIGISSPIGTLSQDITLADRVKHLMLPVFTLSILGIANVTLHTRTKMMSVL
                     SSEYVLFARARGETQWQIFKHHCLRNAIVPAITLHFSYFGELFGGSVLAEQVFSYPGL
                     GSTLTEAGLKSDTPLLLAIVMIGTLFVFAGNLIADILNSIINPQLRRKV"
     gene            complement(7282..8910)
                     /locus_tag="VC394_001465"
     CDS             complement(7282..8910)
                     /locus_tag="VC394_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEP5994922.1"
                     /translation="MSKYLKYFSIITLFLTGLILVACQQQKPQTKERQRKQRPKDELV
                     VSMGAKLPHEFDPKDRYGVHNEGNITHSTLLKRSPELDIKGELAKTYHLSEDGLTWSF
                     DLHDDFKFSNGEPVTADDVKFTYDMLKADGKAWDLTFIKNVEVVGKNQINIHLTEAHS
                     TFTAQLTEIPIVPKKHYNDKYKSNPIGSGPYMVKEYKAGEQAIFVRNPYWHGKKPYFK
                     KWTWVLLDENTALAALESGDVDMIYATPELADKKVKGTRLLDIPSNDVRGLSLPYVKK
                     GVITDSPDGYPVGNDVTSDPAIRKALTIGLNRQKVLDTVLNGYGKPAYSIIDKTPFWN
                     PKTAIKDNKVAKAKQLLTKAGWKEQADGSRKKGDLDAAFDLYYPTNDQLRANLAVEVA
                     EQAKALGITIKLKASNWDEMATKSHDSALLYAGGRHHAQQFYESHHPSLAGKGWTNIT
                     FYNNPTVTKYLDKAMTSSDLDKANEYWKLAQWDGKTGASTLGDLPNVWLVSLNHTYIG
                     DKRINIGKQGVHSHGHDWSLLTNIAEWTWDESTK"
BASE COUNT         2948 a         1761 c         1790 g         2492 t
ORIGIN      
        1 aagatggtag ttgccattga caaagcattg ataaaggagt aaaattaact ggttaataac
       61 tggttaaatt ataattgagg aggtactatg aaaaaaggtt tttttctcat ggctatggtc
      121 gtgagtttag taatgatagc agggtgtgat aagtcagcaa accccaaaca gcctacgcaa
      181 ggcatgtcag ttgtaaccag cttttaccca atgtatgcga tgacaaaaga agtatctgga
      241 gacctcaatg atgtgaggat gatccaatca ggtgcaggca ttcattcctt tgaaccgtct
      301 gtaaatgatg tggcagctat ttatgacgcg gatttgtttg tttaccattc acatacctta
      361 gaagcttggg caagggatct agaccctaat ttaaaaaaat caaaggttga tgtgtttgaa
      421 gcgtcaaaac ctttgacact agatagagtc aaagggctag aagatatgga agtcacacaa
      481 ggcattgacc ctgcgacact ttatgaccca catacctgga cggatcccgt tttagctggt
      541 gaggaagctg ttaatatcgc taaagagcta ggacgtttgg atcctaaaca caaagacagt
      601 tacactaaaa aggctaaggc tttcaaaaaa gaagcagagc aactaactga agaatacact
      661 caaaaattta aaaaggtgcg ctcaaaaaca ttcgtgacgc agcacacggc attttcttat
      721 ctggctaaac gattcggctt gaaacaactt ggtatctcgg gtatttctcc agagcaagag
      781 ccctctcctc gccaattgaa agaaattcaa gactttgtca aagaatacaa cgtcaagact
      841 atttttgcag aagacaatgt caatcccaaa attgctcatg ctattgcgaa atcaacagga
      901 gctaaagtaa agacattaag tccacttgaa gctgctccaa gcggaaacaa gacatatcta
      961 gaaaatctta gagcaaattt ggaagtgctc tatcaacagt tgaagtaaag gagattatta
     1021 gtgaagaaaa catatggtta tatcggctca gttgctgcca ttttactagc tactcatatt
     1081 ggaagttacc aacttggtaa gcatcatatg ggttcagcaa caaaggacaa tcaaattgcc
     1141 tatattgatg atagcaaagg taaggcaaaa gcccctaaaa caaacaaaac gatggatcaa
     1201 atcagtgctg aagaaggcat ctctgctgaa cagatcgtgg tcaaaattac tgaccaaggc
     1261 tatgtgacct cacacggtga ccattatcat ttttacaatg ggaaagttcc ttatgatgcg
     1321 attattagtg aagagttgtt gatgacggat cctaattacc attttaaaca atcagacgtt
     1381 atcaatgaaa tcttagacgg ttacgttatt aaagtcaatg gcaactatta tgtttacctc
     1441 aagccaggta gtaagcgcaa aaacattcga accaaacaac aaattgctga gcaagtagcc
     1501 aaaggaacta aagaagctaa agaaaaaggt ttagctcaag tggcccatct cagtaaagaa
     1561 gaagttgcgg cagtcaatga agcaaaaaga caaggacgct atactacaga cgatggctat
     1621 atttttagtc cgacagatat cattgatgat ttaggagatg cttatttagt acctcatggt
     1681 aatcactatc attatattcc taaaaaggat ttgtctccaa gtgagctagc tgctgcacaa
     1741 gcctactgga gtcaaaaaca aggtcgaggt gctagaccgt ctgattaccg cccgacacca
     1801 gccccagccc caggtcgtag gaaagcccca attcctgatg tgacgcctaa ccctggacaa
     1861 ggtcatcagc cagataacgg tggctatcat ccagcgcctc ctaggccaaa tgatgcgtca
     1921 caaaacaaac accaaagaga tgagtttaaa ggaaaaacct ttaaggaact tttagatcaa
     1981 ctacaccgtc ttgatttgaa ataccgtcat gtggaagaag atgggttgat ttttgaaccg
     2041 actcaagtga tcaaatcaaa cgcttttggg tatgtggtgc ctcatggaga tcattatcat
     2101 attatcccaa gaagtcagtt atcacctctt gaaatggaat tagcagatcg gtacttagcc
     2161 ggccaaactg aggacaatga ctcaggttca gatcactcaa aaccatcaga taaagaagtg
     2221 acacatacct ttcttggtca tcgcatcaaa gcttacggaa aaggcttaga tggtaaacca
     2281 tatgatacga gtgatgctta tgtttttagt aaagaatcca ttcattcagt ggataaatca
     2341 ggagttacag ctaaacacgg agatcatttc cactatatag gatttggaga acttgaacaa
     2401 tatgagttgg atgaggtcgc taactgggtg aaagcaaaag gtcaagctga tgagcttgct
     2461 gctgctttgg atcaggaaca aggcaaagaa aaaccactct ttgacactaa aaaagtgagt
     2521 cgcaaagtaa caaaagatgg taaagtgggc tatatgatgc caaaagatgg caaggactat
     2581 ttctatgctc gtgatcaact tgatttgact cagattgcct ttgccgaaca agaactaatg
     2641 cttaaagata agaagcatta ccgttatgac attgttgaca caggtattga gccacgactt
     2701 gctgtagatg tgtcaagtct gccgatgcat gctggtaatg ctacttacga tactggaagt
     2761 tcgtttgtta tccctcatat tgatcatatc catgtcgttc cgtattcatg gttgacgcgc
     2821 gatcagattg caacaatcaa gtatgtgatg caacaccccg aagttcgtcc ggatgtatgg
     2881 tctaagccag ggcatgaaga gtcaggttcg gtcattccaa atgttacgcc tcttgataaa
     2941 cgtgctggta tgccaaactg gcaaattatc cattctgctg aagaagttca aaaagcccta
     3001 gcagaaggtc gttttgcaac accagacggc tatattttcg atccacgaga tgttttggcc
     3061 aaagaaactt ttgtatggaa agatggctcc tttagcatcc caagagcaga tggcagttca
     3121 ttgagaacca ttaataaatc tgatctatcc caagctgagt ggcaacaagc tcaagagtta
     3181 ttggcaaaga aaaatgctgg tgatgctact gatacggata aacccaaaga aaagcaacag
     3241 gcagataaga gcaatgaaaa ccaacagcca agtgaagcca gtaaagaaga agaaaaagaa
     3301 tcagatgact ttatagacag tttaccagac tatggtctag atagagcaac cctagaagat
     3361 catatcaatc aattagcaca aaaagctaat attgatccta agtatctcat tttccaacca
     3421 gaaggtgtcc aattttataa taaaaatggt gaattggtaa cttatgatat caagacactt
     3481 caacaaataa acccttaacc aaaagaagat ctcattgtta aagcactgct ttgtcaaagc
     3541 aagttaccgt gattttgaag tcattttatg taacgagtag tgataaaagt tggataatag
     3601 cggttttctt ttgcaaagaa atggtatcca tgttagaata ataaaaaaaa gaggaggatt
     3661 ctaatcatgt cagaagaaaa attaaaatca aaaattgagc aagcatctgg tggcctaaaa
     3721 gaaggtgctg gtaagttgac aggtgataaa gagctagaag caaaaggatt tgttgaaaaa
     3781 acaattgcta aaggcaaaga acttgcagac gatgcaaaag aagcggttga aggtgctgta
     3841 gatgccgtta aggaaaaatt aaaataatta aaaaaaccag ctgtaagctg gtttttttaa
     3901 tggtgtgatt ttgttaggaa aagactcaat aggtaataac cgtaggtgtt aggtatgctt
     3961 gaacagttga gtacgattct cttcaatcat atagcacgcg tcacaaagtt tttctaacat
     4021 atcaaactca tgactaataa tgatgagacc taaatttcta tcctttacaa tctccaacag
     4081 gcttttccat acactagctt gtgtaatgct atccaacata gtagtcattt catctgcaat
     4141 aaggtatttc gtctctggat gtaatgaacg cacaatcgaa aagcgttgca attcccctcc
     4201 cgagagttca ctagggcgac gctttagcca tttttcttga atgccaaaag catctcgcaa
     4261 atcttgactt ggatagtagg cttcttccaa actttttttc ataggccata aaggattcat
     4321 ggtttgttca ggatgttgtt ggataagttg tacaggtcta aatgctttgt taggcaaatg
     4381 actgccatca actaatactt caccagattt aggctgcaaa aaaccggcaa gcactctgga
     4441 taaacttgtt tttccacacc cactttgtcc aaatatccct aagatttgac caggtgctac
     4501 ctctaaatca atctccttaa aaagccattg atctttttta tgataaaagc caagcttttt
     4561 agcctctaag gtcatgagta actcctttca aaaagtcttg ctgagggagt gtgcgccata
     4621 aacgtctcgc aaattctgtt tgtaattgct ccccacctcc gctaaaaaag ctagctgggg
     4681 ctgtttcaat agcttttccc tctttaaaaa tagttatacg atccgcaatt tgactagctg
     4741 ctacaatatc atgagtgata aatatgacgc ttattccttt atctgcaaag gagcgtagtt
     4801 gatctaaaac catttgcaga gcatctggat gtaatccagg ggtgggctca tccgcaataa
     4861 tcaaagaaac cgtatcacta atacacgttg taaacaaaac acgtcggagc attccgccag
     4921 aaagttggaa aggatacaag ttaccatcac tttcttttaa accaaactgt tgaaacaatc
     4981 cttcttgagt agccttagca ttttctgaga tacccaagcg cacctgatgc ttgactttca
     5041 tagatggatc taaataatta acggattgtg gaatcaacgt catttctttt cctcgcaact
     5101 gtttgatgcg tttagaagtt agtgattgac cacgataaat catatctcct gttacagctg
     5161 catttttagg aagaatatcc ataatagcat gtgctaataa actcttgcct gaaccactag
     5221 cacctataat agctaataac tcaccttttt taacttctaa attcagcgct tggatcggtg
     5281 tggattgaaa tggttttaaa aatcttccgt attgagtgaa ggtgatggag aggtctttaa
     5341 tgcttaataa tgtttctgtc atatctctac ctcctaaaaa tgatccgttt gagggtaaaa
     5401 gagtttcttt aaagattctc cgatagtatc aaaggcattg acaactaaaa taagataaag
     5461 gcctggaaaa accaccaacc accaattgcc aagagagata tgcttagctg cttctgacag
     5521 aatgatacca accgaaggtt gttcggcaga aaggccaaat cctaagaaag tcatggatgc
     5581 ttcatgcaag atgacgtgcg gaaataagag gataaaccca atgaaaattt gagaagcaat
     5641 caaaggcagg atatgatgcc tcacaatata ataaggcgtt tttcccatgc ttttagagag
     5701 ctggacaaag gctttattct ttagatcata gacttcattg cggataagcc ttgctagaga
     5761 gggccaatgg gtaacagccg ttgcaatgat aaccccttga gcccctttac caacaacaaa
     5821 agaaatgaga atcataaaaa tcaaatgagg cataccaata aacaaatcaa ccaaccaggc
     5881 tattatttta tcaataaggc tatttcctag acctgcaagc actccaaaaa cggtcgcaag
     5941 agagactccc ataagggcac ctaataagcc gacttgtaaa gagaaataaa gccctttaat
     6001 cgttctgacg aacatatccc tacctaaacc atctgtccca aaaagatggt ttaaggaagg
     6061 agcgaggttg cgtaaagatg cattggtttc caaaggcgtc ctatagaaat aaaggtttaa
     6121 ggctagaata ctaagaatga gagaaatggc gatacctagt tgccataaaa ccatcgttcg
     6181 acgtttcaat atcatacttt tctccttaac tgtggattga tgatgctatt taaaatatcc
     6241 gcaataagat tgcccgcaaa aacaaataat gtccctatca tcacgatagc tagaaggagc
     6301 ggtgtatcac ttttaagtcc tgcttcagtg agggtagacc ctaagccagg atatgagaaa
     6361 acttgctcag caagaacaga tcctccaaac aattctccaa aataggaaaa atgcagtgta
     6421 atagctggta cgatagcatt tctaaggcaa tgatgtttaa aaatttgcca ctgtgtttcc
     6481 ccacgcgctc tggcaaataa gacatattca ctagaaagca ccgacatcat tttagttctc
     6541 gtatgaaggg tgacattggc aatgcctaga atacttaacg tgaaaacagg taacataagg
     6601 tgcttaaccc gatcagctaa cgtaatatct tgactcaaag tgcctatcgg ggaagaaata
     6661 ccaatcggga accaccccag ctggacagaa aagattaata aaaaaatgag gccaatccaa
     6721 aacgttggta ctgatatctg aaggtaagaa aaccacctga caactcgatc aagtaatttc
     6781 ccttgatgga aagcggataa cgttcctaag ataaatccaa taagacccga taagatccaa
     6841 gagagtccca taagtatgaa agaagcacct gcccgtgatc taataatatc actaacaggt
     6901 tgccgataaa cgagcgaggt ccctaaatgt ccctgtatca catttttcaa ccaaataaaa
     6961 tactggacta gagctggctt atccaagcca tagtggtgag caatcgcttt gtactgagca
     7021 ggggttagtg atgtgtcata attgacactt gccatgactg gatctactgg agattgtttt
     7081 aacaaaacga aggtcaaaac agatacccca aaaataagcg tgacacatct gatgattttc
     7141 cagatgataa taatggtagt acgtttcaca atgaaatctc cttaatcgat aagaaaagga
     7201 gccgaggaaa cgatcagcac agttttgtgt atcttgtttt ctcagactcc aaacttaaag
     7261 tcgctgtatt aactaaacgc gttacttagt tgattcatcc caagtccact cggcaatgtt
     7321 agtcaataat gaccaatcat gaccatgact gtggacgccc tgtttaccta tattgatacg
     7381 tttatcacca atataagtat ggttaaggct caccaaccat acatttggca aatctccaag
     7441 agtagaagca cctgttttgc catcccactg cgctaacttc caatattcgt tagctttgtc
     7501 aaggtcagaa gatgtcattg ctttgtcaag gtacttagtc acggtaggat tgttataaaa
     7561 cgtaatattg gtccaacctt tacctgctag gcttggatga tgtgattcat aaaattgctg
     7621 cgcgtgatga cgtcctccgg cataaagtaa ggctgagtca tgtgacttcg ttgccatttc
     7681 atcccagtta ctagctttga gtttaatagt aatccctagg gctttggctt gctctgctac
     7741 ttcaacggct aagttcgctc gcaattgatc attagtaggg tagtacagat caaacgctgc
     7801 atcaaggtca ccttttttac ggctaccgtc tgcttgttct ttccatccag cttttgtcaa
     7861 caattgctta gctttagcta ctttattatc tttaatggct gtttttggat tccaaaatgg
     7921 tgttttatca ataattgaat aagctggttt accataacca tttaaaaccg tatcgagaac
     7981 tttttgccta tttaaaccaa tagtcaaggc ttttcggatt gctggatcac tagtgacatc
     8041 atttcctaca ggataaccat caggagaatc agtgatgacg ccttttttca cataaggtaa
     8101 tgataagccg cgcacatcat ttgatggaat atcaaggagg cgggtgcctt tgactttttt
     8161 atcagcaagt tctggcgttg cgtagatcat atcaacatca ccagattcta aagctgctag
     8221 tgctgtgttt tcatcaagta agacccaagt ccatttttta aagtatggtt ttttcccatg
     8281 ccaataaggg ttacgaacaa aaatagcttg ttctccagcc ttatattctt ttaccatgta
     8341 aggtcctgaa ccgataggat tgctcttata cttatcattg taatgttttt tagggacgat
     8401 tgggatttca gtcaactgtg ctgtaaatgt cgaatgcgcc tcagtcaaat ggatattgat
     8461 ctgatttttc ccaactactt caacgttctt gatgaaggtt agatcccaag cctttccatc
     8521 tgctttcaac atatcataag taaacttaac atcatcagca gtaacaggct caccatttga
     8581 gaatttaaaa tcatcatgca agtcaaacga ccaagtcagc ccatcttcag agagatggta
     8641 tgttttagca agctctcctt ttatatctag ttcaggagaa cgtttcaata gagtgctatg
     8701 agtgatattc ccttcattgt ggactccata acggtccttt ggatcgaatt catgagggag
     8761 ctttgccccc atagaaacga caagttcgtc ttttggacgt tgtttgcgct gacgttcttt
     8821 tgtttgaggc ttttgttgtt gacatgcaac taaaataagc ccagtcaaaa ataacgtgat
     8881 aatagagaag tattttaggt attttgacac aatcaaatct ccttatttct aattaataaa
     8941 taataatagt aacgactcca tctcattata gcaataaaca aataggaggg c
//
LOCUS       DATXQK010000052         8956 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.052, whole genome shotgun sequence.
ACCESSION   DATXQK010000052 DATXQK010000000
VERSION     DATXQK010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8956
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.052"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..615
                     /locus_tag="VC394_001466"
     CDS             <1..615
                     /locus_tag="VC394_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEP5994923.1"
                     /translation="VQRLMKVMSLAARIRRKRKYSSYKGEVGKKADNLIKRHFEGSKP
                     YEKCYTDVTELALPEGKLYLLPVLDGYNSEIIDFTLSRSPNLKQVQTMLEKTFPADSY
                     SGTILHSDQGWQYQHQSYHDFLESKGILPSTSRKGNSPDNGMMDSFFGILKSEMFYGL
                     ETTYQSLDKLEEAITDYIFCYNNKRIKAKLKGFSPVQYRTKSFQ"
     gene            complement(664..1395)
                     /locus_tag="VC394_001467"
     CDS             complement(664..1395)
                     /locus_tag="VC394_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEP5994924.1"
                     /translation="MRLDKFLVATGVGTRSQVKLLLKKKAIFVNQKVETSAKAHIDEY
                     KDLVTYQGTPLVYESFVYYLLNKPSGYVSATQDRQQATVMELLDDTARQKAVFPVGRL
                     DKDTRGLLLLTNNGQLAHDLLSPKKHVTKEYLAKVVGIMTEADKDYFARGISLKDHQC
                     LPAHLEVLASDLQQRTSLVKITIQEGKFHQVKRMVAACGKGVLDLQRLSMGPLKLDPS
                     LAEGEFRRLTPEELQSLAPYCQSSL"
     gene            1510..1875
                     /locus_tag="VC394_001468"
     CDS             1510..1875
                     /locus_tag="VC394_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEP5994925.1"
                     /translation="MTIYSHTYETRPNHSAKAMGSGTLNVLATPALVAFMENASYLFA
                     QEQLDSDFTTVGSEIAIQHLAASAIGDPVTIIITALKEEGRKYDFRLEAFVGEQLIGK
                     ACHTRVRVNSKHFMAKVAE"
     gene            complement(1995..3215)
                     /locus_tag="VC394_001469"
     CDS             complement(1995..3215)
                     /locus_tag="VC394_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEP5994926.1"
                     /translation="MSTLSLDTTNKRALVAAIAASGTDDLNVMFLAFSMSSIMTDLSL
                     SGTQGGWIATITNLGMLVGGLLFGLLADRHHKFKVFKWTILLFSVATGLIYFTQSLPY
                     LYLMRFIAGIGVGGEYGVAIAIMAGIVPPEKMGRMSSLNGIAGQLGSISSALLAGWLA
                     PSLGWRGLFLFGLLPILLVIWMTLAIDDQKIWDHYGQEEEECSQPIKINELFKTKSLT
                     AQTLALMVMTTVQIAGYFGMMNWLPTIIQTSLNLSVKSSSLWMVATIVGMCLGMLCFG
                     QLLDCFGPRLIYSLFLLASSICVYLFQFANSMASMVIGGAIVGFFVNGMFAGYGAMIT
                     RLYPHHIRSTANNVILNVGRALGGFSSVAIGSILDASGISMVMIFLASLYVISFGAMW
                     SIGQLKAERYQQLR"
     gene            3589..5073
                     /locus_tag="VC394_001470"
     CDS             3589..5073
                     /locus_tag="VC394_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEP5994927.1"
                     /translation="MKTIAFDSNKYLNLQRDHILERISQFDGKLYMEFGGKMLEDYHA
                     ARVLPGYEPDNKIKLLKELKEQVEIVIAINANNIEHSKARGDLGISYDQEVFRLIDKF
                     NTLDIYVGSVVITQYNNQPAADAFRKQLKKNGIASYLHYPIKSYPTDINHIISSEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCISQMYHDQINGVKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLETYGKTAVNYNRDIEVFPVLNRTFERILSKSPYA
                     SPTDMGVNMVGFSIVNEEAAIEASKQEIIRRYYQTLVDFKAERVTESAVKKIELLMND
                     IGVTPDDRHVTVAAHQKAEQTGQPALALQLPNGQIVTGKTSELFGPTAAVIINAIKTL
                     AKIDKTTHLIEPEYVKPIQGLKVNHLGSHNPRLHSNEILIALAITAMTSEEANLAMKE
                     LGNLKGSEAHSTVILTEEDKNVLRKLGVNITFDPVYQHHKLYRK"
     gene            complement(5178..5579)
                     /locus_tag="VC394_001471"
     CDS             complement(5178..5579)
                     /locus_tag="VC394_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEP5994928.1"
                     /translation="MSDLTQEMTLNVISIFDNYQIELAEKGHLILSTEVTETALNYYG
                     NAHGGYLFTLCDQVGGLVARTTGVESVTLQANANYLKAGHKGDKLMVEGRLVHGGRTT
                     QVVDVTIHNQTGALLTKTSLTMFVTGRRHNG"
     gene            complement(5664..6445)
                     /locus_tag="VC394_001472"
                     /pseudo
     CDS             complement(5664..6445)
                     /locus_tag="VC394_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060407.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
     gene            complement(6597..8276)
                     /locus_tag="VC394_001473"
     CDS             complement(6597..8276)
                     /locus_tag="VC394_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEP5994929.1"
                     /translation="MSKEKIKVYLYTRVSTSIQIEGYSLEAQKSRMKAFAIYNDYEIV
                     GEYEDAGKSGKSIEGRKQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIM
                     QDYGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRIQTMEGRIQKAREGKWNG
                     GFAPYGYKLEDGKLFINEEEAVAIRTIFDQYVNTTIGANGLSKYLENHGIRKIPRQNG
                     KNPLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEAI
                     VSDEVWQAAQVKLKSQAKKYEHVNKGKDTRTHLLSGIVKCPICGAGMFGNKCTKKKKD
                     GTKYKDFYYYGCKHRQMIRGHKCTFRKQIREELLDDAVAEVIVKIVSNPKFASMMQEK
                     INMKVDTSEIEKEIDNYQKELRKSHSTKFKIIEEIDNLDVEDKHYKRRKQDLDDRLYR
                     MYDKIDELESSLIDAKAKKQTIEAEKLTGDNIYKVLIYFDKLYKVMNDVERRQLITAL
                     ISEIQVYEEKQPNGQWLKSITFKLPIIDDDLNISLDNNEQVECVSLLEKRS"
     gene            complement(8373..8534)
                     /locus_tag="VC394_001474"
     CDS             complement(8373..8534)
                     /locus_tag="VC394_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994930.1"
                     /translation="MDNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFD
                     KAKTQIETD"
BASE COUNT         2699 a         1949 c         1523 g         2785 t
ORIGIN      
        1 gttcaacgtc tcatgaaagt catgagccta gcagcccgca ttcgtcggaa gcgcaagtat
       61 tcttcttaca aaggagaggt tggtaagaag gctgataatc tgattaaacg tcacttcgaa
      121 ggctctaaac cttacgaaaa atgttacact gatgtgacgg agttggcctt acctgaagga
      181 aaactctact tattacctgt tcttgacggc tataacagtg agattattga tttcactctg
      241 tctcggtctc ctaacttgaa acaggttcaa accatgcttg agaagacttt tccagcggat
      301 tcatacagcg gaactattct tcatagtgac caggggtggc aataccaaca ccagtcctac
      361 catgattttt tagagtctaa aggtattttg ccatccacgt cccgcaaagg gaatagcccg
      421 gataacggca tgatggattc cttctttggc attctcaaat ctgaaatgtt ttatggactc
      481 gagacaactt atcaatcact tgataagctt gaagaagcta ttacagatta cattttttgc
      541 tacaacaaca aacgaattaa agcaaaacta aaaggattta gtcctgtcca atacagaact
      601 aaatcctttc aataattatt tgtccaactt tttggggtca gtactgacag gggaggtttc
      661 tttttatagt gagctctgac aataaggtgc caggctttgc aattcctcag gcgtcaggcg
      721 cctaaactcc ccttctgcaa ggctagggtc tagttttaat ggtcccatgc tgagacgctg
      781 caagtcaagc actccctttc cacaggctgc taccatgcgt ttcacctgat ggaattttcc
      841 ttcttgtatg gtaattttaa ccaaacttgt tcgctgctgt aaatcactag caagcacttc
      901 taaatgagca ggtaggcact ggtgatcctt taaggaaata cctctagcaa aataatcttt
      961 atcagcttca gtcatgatgc ctactacttt agccaagtac tctttcgtaa catgtttttt
     1021 aggggataaa aggtcatggg ctagttgtcc attattggtc aagagcaaaa gtccacgggt
     1081 atccttatct aacctaccca caggaaaaac tgctttttgc ctagccgtgt catctaacaa
     1141 ctccataaca gtagcctgct gtctgtcctg ggttgctgac acataccctg acggcttgtt
     1201 taacaggtaa taaacaaagg attcatagac caagggcgtg ccttgatacg tcaccaaatc
     1261 cttgtattca tcaatgtgtg ctttggcaga ggtttcaacc ttttgattga cgaaaatagc
     1321 cttctttttt aagagaagtt tgacttggct cctagttcca acaccagttg ccactaaaaa
     1381 tttatctaag cgcatcataa ccctttcaaa gaaaattcat tattaagtat aacataaaaa
     1441 aacagtagtt tcatgactgt aagcgcttaa tatattataa taggcttata gaaacgagga
     1501 gattaactta tgactattta ttctcacact tatgaaaccc gtcccaatca ttcggctaaa
     1561 gccatgggct caggaacgct gaatgtccta gctactcctg ctcttgttgc ttttatggaa
     1621 aatgcttctt acttatttgc ccaagaacag ctcgacagtg actttacaac ggttggcagc
     1681 gagatagcta tccaacattt ggcagcttca gccattggag atcctgttac aatcatcatt
     1741 acagccctta aagaagaagg ccgaaaatac gatttccgct tggaagcttt tgtgggagaa
     1801 caactcattg gaaaagcctg tcatactaga gttagggtga acagtaaaca ttttatggcc
     1861 aaagtagcgg agtaaaacaa aagaaaagaa ccaaaaaggt aagcaatccc taagtgctta
     1921 cctttttggt tatctcaatc aataaaatag ctttgtgagt gaaagtaact caaatactaa
     1981 cacccatctc tttgctaacg caattgttgg tagcgttctg cttttaactg accaatagac
     2041 cacatagctc caaaactaat cacatacaga gaagctagga aaatcatgac cattgaaata
     2101 cctgaggcat ctaaaatact accaatagca acggacgaaa atcctcctag agcacggcca
     2161 acattcaaaa taacattatt agccgtggat cgaatgtgat ggggataaag tctggtaatc
     2221 atagccccgt agccagcaaa catcccattg acaaaaaaac cgacaatagc acctccaata
     2281 accatactcg ccatggaatt ggcaaattga aaaaggtaaa cacaaataga tgatgctaat
     2341 aaaaagaggg aataaatcag acgtggacca aagcaatcta aaagctgacc aaaacacagc
     2401 atccccaaac acatgccgac aatagtagcc accatccaca aggacgaact ttttactgaa
     2461 aggtttaagc ttgtttgaat aatggttggc aaccaattca tcattccaaa atagcccgca
     2521 atctgcacag tcgtcattac cattaaagca agggtttgag ctgttaagga ttttgttttg
     2581 aatagttcat tgattttaat tggttgactg cattcttcct cctcttgccc ataatgatcc
     2641 caaatttttt ggtcatctat ggctaaagtc atccaaatca ccaagagaat cggaagtaat
     2701 ccaaagagaa aaaggccacg ccagcctaga cttggagcca gccaccctgc taaaagagcc
     2761 gaactaatgg agccaagctg gccagcaata ccatttaaag aagacatccg acccattttt
     2821 tctggtggta caatccctgc cattatggca atggctacac cgtactcacc tccaactcct
     2881 atgccagcaa taaatcgcat caggtacagg tagggcaaag actgggtaaa ataaattaat
     2941 cctgttgcta ctgaaaacag taaaatcgtc catttaaaga ccttaaactt gtggtgccta
     3001 tcagccagca acccgaataa gaggcctccc accaacattc ctaaattggt aatggtcgca
     3061 atccagccac cctgagtgcc actaagactc aagtctgtca taatggaaga catggaaaag
     3121 gcaagaaaca tgacattcaa gtcatccgtt cctgaagctg caatagctgc cactaaagct
     3181 cgcttatttg tggtgtccaa tgataatgtt gacataattg tctccttctt aatagtagct
     3241 cactgactac cttaattaat tgaataccct ttgtccaaag gtcaaaaaaa gacttggaaa
     3301 caagtccgca agtccatcaa agttcactaa ataataagag atgacaaaaa aactctcttt
     3361 aatgttaaga ctttcatgct tcacggaaca tgtttaaagt agtatttaat tgttactagt
     3421 atagtaaagc tcattttaat tgtcaagttc ttttgaacat agcggataca tttttcttct
     3481 aagaaccaag cctaagtttt tttaaaaagt cacaaaatga ggtatccctg caaaaaacct
     3541 tttctttctt ggaatttctt tgttataatg ctttacagag gtgaaaaaat gaaaactatt
     3601 gcttttgatt ctaacaagta tttaaacttg caaagagatc atattttaga acgtatcagc
     3661 caatttgatg gcaaacttta catggaattt ggtggtaaaa tgttagaaga ttaccatgct
     3721 gcccgtgtac ttcctggcta tgaacctgac aataagatta aattactcaa agagctcaaa
     3781 gaacaagtcg aaattgtaat tgccattaac gctaataaca ttgaacattc taaagcgcgt
     3841 ggtgatttag gcatttctta tgaccaagaa gtctttcgct taattgacaa atttaacacg
     3901 ttagatattt acgtaggttc tgtcgttatt acccaataca ataaccaacc cgctgcagat
     3961 gcctttcgca agcaactcaa aaaaaatggc attgcttctt acctgcatta tcctatcaag
     4021 agctacccga cagatattaa tcatattatc tcatctgagg ggatggggaa aaatgattac
     4081 atcaaaacga gtcgtaacct tatcgttgtt acagcaccag gccctgggtc tggaaaatta
     4141 gccacttgta tatcccaaat gtatcacgac caaatcaatg gggttaagtc tggctatgct
     4201 aaatttgaaa ccttcccagt ttggaatctt cctcttcatc accctgtcaa tctggcttat
     4261 gaggcagcca cggcagacct agatgatgtg aatatgattg accccttcca tttggaaact
     4321 tatggcaaaa cggccgttaa ttacaaccgt gatatcgagg tctttcctgt cttaaatcgg
     4381 accttcgagc gtattttgag caagtctcct tatgcctcac caaccgatat gggtgttaac
     4441 atggttggtt tttctatcgt caatgaagaa gctgctatcg aagcttctaa acaagaaatc
     4501 atccgtcgtt attaccaaac cctcgtggat tttaaggcag agcgggtcac agaatctgct
     4561 gtcaaaaaaa ttgagctctt gatgaatgat atcggtgtca cccctgacga tcgtcacgtt
     4621 actgtagctg ctcatcaaaa agcagagcaa acaggccaac ctgcccttgc tctccaacta
     4681 ccaaatgggc aaattgtgac aggtaaaaca tctgaactct ttggaccaac tgcagctgtt
     4741 atcatcaatg cgattaaaac cttggcaaag attgataaaa ccactcattt gattgaaccc
     4801 gaatacgtga aacctattca agggctcaaa gtgaaccact taggcagtca caatccgcgg
     4861 cttcattcca atgaaattct gattgcgtta gccattactg ctatgactag tgaagaagct
     4921 aatctagcca tgaaagaact cggcaatttg aaaggtagcg aagcccattc taccgttatc
     4981 ttaaccgaag aagataaaaa tgtactccga aaacttgggg taaatattac ttttgaccct
     5041 gtttaccaac atcacaaact ttaccgtaaa taaactataa ccacccgtct aacgggtgat
     5101 ttatggtatc tctcaataaa ggaaaaaagt cccaattctc agagagttgg gacttttttc
     5161 ctttattgat tcattaatca cccgttatga cgtcggccag tcacaaacat ggtcaagctt
     5221 gtttttgtta gaagagctcc tgtttggtta tgaatcgtca catcaaccac ctgcgtggtt
     5281 cgacctccat gaaccaatcg accttcaacc atcagcttgt ctcccttgtg ccctgctttt
     5341 aaatagttag cattggcctg taaggtgaca gattctacgc cagtagttct agcaaccaaa
     5401 ccacctactt gatcacatag agtgaaaaga taaccaccat gagcattccc atagtaattc
     5461 aaagccgtct cagtcacttc agtacttaaa ataaggtgtc ctttttcagc aagttcaatt
     5521 tggtaattat caaaaatact aataacattt aaggtcatct cttgtgttaa atctgacatt
     5581 gtttttctct tttctaagct tagtctaaaa gcaatacaag cctcattata ctacaaatag
     5641 gaacagaagg cagagctcat gtttcaaaaa tcagtagcct ttccatcctt ttctagcgta
     5701 ttgaacactt ccaagcataa atgaagcgca aaactaaagg aatcagcgcc ccggttgcgt
     5761 tgatcgtaaa cctctacatc tgctagagta tctgctgtaa ataagagttg tccccaaagg
     5821 atgcctctta attgagctac tgctgctaag gcagaacatc ccatctcaac aaccgaacac
     5881 ccttcttcct ggcgataagc cactttttct ttggtttctc ggtaaaaacc atctgttgac
     5941 caggtgatga cctcttgata agctaacccc tgtgctgaaa gcgttttctc tatcggtcta
     6001 agcatcttag gatcaatatc aatataacaa gatggtgcca cgtaatgata acttgttcct
     6061 tcatctcgca aagccttaac tggcactaaa aaacggtttt ctgcgatagg aaccaaaacg
     6121 ccacaggttc ctgtactgat catttcttta accccataac caatcagcca gtccatgaat
     6181 tgcgccgcag gagctgagcc aacgggagct tgagctaggc aaatgtcttg gcctttataa
     6241 ggtatctgat aaacagggta aacctttgtg gctgaaatga attctgctac ttttttacct
     6301 tgatggtcta aagcaaaagc ttcaatctcg tcttctaaga aagcatagac acacttagct
     6361 gtagaccttc atgtgtaggt ataatgacgg ctgatggatt actatcacat tctaaaaggg
     6421 gaatctcgtg tttggtaata ggcattcgtt tctccttatt tttgtcatgt ggaggcagct
     6481 gccttactcc ttgattagca acactacgca ctcgacgtga tgggtctgcg ggagtcaaaa
     6541 ggtccagtgg accttttgag cgtctcacct tgaaataaca gagtgaggcg gactctttaa
     6601 ctccgtttct caagtaaact tacacactca acttgttcat tattatccaa acttatattc
     6661 aagtcatcgt ctataattgg gagcttgaaa gtaattgatt ttagccattg tccgttaggt
     6721 tgtttttctt cataaacttg aatttcagaa atcaaagctg taattaactg tctacgctct
     6781 acatcattca tgactttata gagtttatca aaatagatca gaaccttata tatgttatct
     6841 cctgtgagtt tttcagcttc aatagtctgt ttctttgctt tcgcatcaat tagtgatgat
     6901 tctaattcat ctattttgtc atacatacga tatagtctat catctaaatc ctgtttcctt
     6961 ctcttatagt gcttatcttc aacatctaaa ttatctattt cctcaattat cttaaacttt
     7021 gtagaatgac tcttcctcaa ttccttttgg taattatcta tttctttttc tatttcagag
     7081 gtgtccacct tcatgttgat tttttcttgc atcatagaag caaatttcgg attacttaca
     7141 atcttgacaa tcacctctgc aacagcatca tctaacaatt cttctctaat ttgcttacgg
     7201 aatgtacact tatgacctct tatcatctgc ctatgtttac aaccatagta ataaaaatct
     7261 ttatactttg tgccatcttt ctttttcttg gtacacttgt ttccaaacat tcccgctcca
     7321 catatagggc attttacaat tccagaaagc aagtgcgtgc gtgtatcttt tcctttattc
     7381 acatgctcat atttctttgc ttgagatttt agcttaacct gagcagcttg ccaaacttcg
     7441 tcggaaacta tagcttcatg tataccttca gatattagat attcatcttg ttcaacctgc
     7501 ttatattcat ttcttgtacc atgaactttt tctaaagttc ttcttccaaa tgctattttc
     7561 ccattatata caggattctt taatatcttt cttataagtc ctgcatcaaa caaaggattc
     7621 tttccattct gtcttgggat ttttctaatt ccatgattct ctaagtattt agatagccca
     7681 ttggctccta tcgtagtatt tacatactgg tcgaaaatcg ttcttatggc aactgcctct
     7741 tcctcattta taaacagctt gccatcttca agtttatatc catacggagc aaagccacca
     7801 ttccattttc cttcccttgc tttttgaatg cgaccttcca ttgtttgaat acggatgttt
     7861 tctctttcta tttcagccac agctgataaa acagaaatca ttagtttccc agcatcttta
     7921 gatgaatcaa tgccatcttc aacgcagata agattaactc cataatcctg cattatctga
     7981 agtgtagaaa gaacatcagc ggcatttctt gcaaatcttg ataacttaaa cacaagaaca
     8041 aaagacactc catcttttcc agattttata tcttccatca ttcgattgaa ctgttttcta
     8101 ccttcaatag acttgccaga cttcccggca tcttcatact ctccgacaat ttcataatcg
     8161 ttgtaaatag caaaagcttt cattcgtgat ttttgtgcct ctaacgaata cccctctatc
     8221 tgtattgacg tagatactcg tgtatagagg tatactttta ttttttcttt tgacatagca
     8281 ttaacctcat tataactttc tatatcatat ataaactttg atttttgaat ttaagtttgg
     8341 actatcattt caagtatatt ataacacttt tattagtccg tctcaatttg tgtttttgcc
     8401 ttgtcaaaac tatttttcat ctcttgattt tttgctggcg ttggatcggg cagattatct
     8461 aaatctaaag caccagcata ttttgcaatc agatttgcta ttaaatcagc caatccattc
     8521 cagtcattgt ccaatatata cctcctctct ataaagtttt atatctaata attatttgtt
     8581 taattaagtt ttacgacatt gacaagtgct ttggattagc aacataggaa tctcacctcc
     8641 gcctctattc cggatgagcc ggcttcaacc ttagaagtat cattatcctc attttcttca
     8701 tagcggatag ggtatccctc cctatattca aactcttact tatcgctctc tttcttcttc
     8761 ctttttttgc ttagcagtac ttgttttgcc gaattattca gcagaaagat cttgacggat
     8821 aggttattac gctccaaaaa taattaacgt cttgtcttgg tctattcaat tgttaaggtt
     8881 caaaatttat cgagagttat taatcttttt aaaatttgac catcagagaa aatttatctt
     8941 gatgtaacaa aattct
//
LOCUS       DATXQK010000053         8770 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.053, whole genome shotgun sequence.
ACCESSION   DATXQK010000053 DATXQK010000000
VERSION     DATXQK010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8770
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.053"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..326
                     /locus_tag="VC394_001475"
     CDS             <1..326
                     /locus_tag="VC394_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994931.1"
                     /translation="DNMVVPSPYGDIVYRFIKELINNAIKYGDGKDIRLSLTIQSDII
                     IIEENNQVVEKVHSISYGRGLKSFQETLAAFDGDLELQMDTKQFTIRILLPIDWKLCY
                     EDFIN"
     gene            307..912
                     /locus_tag="VC394_001476"
     CDS             307..912
                     /locus_tag="VC394_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP5994932.1"
                     /translation="MKILLIDDHRLFAKSIQLLFQQYDEVDVIDTITSHFNDVTIDLS
                     KYDITLLDINLTNISKENGLEIAKELIQSTPHLKVVMLTGYVKSIYRERAKKVGAYGF
                     VDKNIDPKQLISILKKVDSGKKYFEQIESQDYVESLTDQEIAILNLSKKGFSIKEIEE
                     TLQISRRTVFNHLTHIYSKLLVNNKQEAIYKAEQLGYFMDF"
     gene            complement(1464..3044)
                     /locus_tag="VC394_001477"
     CDS             complement(1464..3044)
                     /locus_tag="VC394_001477"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEP5994933.1"
                     /translation="MAEKTTSLRQALWHSADQLRGQMDANDYKNYLLGLIFYKHLSDK
                     LLFAVCDNLEEHFNTFTDAQKIFENAYQDEGLKDDLISVVTGDLGYFIEPTLTFEKLI
                     QDVYHNTFQLESLAQGFRDIEQSGEDFENLFEDIDLYSKKLGSTPQKQNQTISNVMKT
                     LNEIDFEAVDGDTLGDAYEYLIGEFASESGKKAGEFYTPQAVSHLMTQIVFLGWEDQK
                     GMTLYDPAMGSGSLLLNAKKYSNQSDTVSYYGQEINTSTYNLARMNMMLHGVAIENQH
                     LSNADTLDADWPTDEPTNFDGVLMNPPYSLKWSATAGFLTDPRFSSYGVLAPKSKADF
                     AFLLHGFYHLKNTGTMAIVLPHGVLFRGAAEGKIRQKLLEQGAIDTIIGLPSNIFYNT
                     SIPTTIIILKKNRTNKDVFFIDASKEFDKGKNQNTMTDSHIKKILDAYKSRDNSDKFS
                     YLASFDEIIENDYNLNIPRYVDTFEEVPVKPLPELAKQLSDIDQEIAKTNAKLDQLMK
                     QLVGTTKEAQDELDTFRN"
     gene            complement(3057..4244)
                     /locus_tag="VC394_001478"
     CDS             complement(3057..4244)
                     /locus_tag="VC394_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEP5994934.1"
                     /translation="MTKSKRPQYRFDSFEGEWEEKKLGEISNIVRGASPRPIQDPKWF
                     DAKSDIGWLRISDVTEQEGRITYLQQRISELGQEKTRVLKDPHLLLSIAATVGKPVIN
                     YVKTGVHDGFLVFLDPKFNREFMFQWLDMFRPYWNKYGQPGSQVNLNSEIIRNQVINL
                     PSLPEQEAIGELFQTVDQLIQLQRQKLAILKEQKQTFLRKMFPAQGQKVPEIRLQGFD
                     GEWEEKELGDIVQITMGQSPSSQNYTTNPSDYILVQGNADIKNGYVFPRVWTTQITKQ
                     ADKGDIILSVRAPVGDVGKTNYHVIIGRGVAAIKGNEFIFQILKYLKEIGYWKRISTG
                     STFDSISSSDIKYAKIQIPSLSEQEAIGNFFQTLDQQIAQSEEKLTELKALKQTLLNR
                     LFV"
     gene            complement(4257..7235)
                     /locus_tag="VC394_001479"
     CDS             complement(4257..7235)
                     /locus_tag="VC394_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEP5994935.1"
                     /translation="MGNQTKTELELEKELIHLLETGESQWTYRKELKTEDALWDNFFK
                     ILAQNNTQYLNEEPLTASEKEQIKNQLNFVNYYEAAKWLAGENGIAKVQVQREDAKLG
                     TIRLEVVKADNVAGGTSVYEIANQVAFSGSRDRRGDVTLLINGLPMIQIELKSQNHQC
                     IEAFNQVKKYDKEGQFRGIFSTLQMFVVSNKTDTRYIAAAKENKLNPNFLTQWVDQNN
                     KPQKDLFAFAKEVLSIPRAHQMVMTYSVIDDDKKALILLRPYQIHAIEAVAEASRHRK
                     SGYIWHTTGSGKTLTSYKVARNILQIPAVEKSIFVIDRKDLDNQTASAFQSYAQNDIF
                     DVDETEDTRQLIKNLESSDRRVVATTIQKLNAMISQMESYDTPKFKKLKERLAHLNVV
                     FVVDECHRAVTPERQRYLTNTFRNSRWYGFTGTPIFVENKRAQLGDLAQTTEQQYGKC
                     LHQYTVKEAIHDKAVLGFQVEYKTTIPDMPEDSIPEEAYDHEEHMLAVLDSIINQSRK
                     KLGFNNGIGQTFEGLLTVKSIARAQAYYDLMKKVKAGETDLVISKKVKEKLPDFPKVA
                     ITYSITENDNASISRQDKMTKNLEDYNHLFGTNFTIDNLQGYNRDLNDRLARKKDKFK
                     DRHEQLDLVIVVDRLLTGFDAPCLSTIFIDRQPMKPQHIIQAFSRTNRIFESRKHYGQ
                     VVTFQTPLRFKEAVDTALSLYSNGGQSEVLAPSWEEEKARFFEKVTVLKNIVPDPDAF
                     PTIESAQTAFLKQYAKAFQAFDKLYASVQVYSDFNETLLSEVGLSDEVIDTYKGTYQN
                     VIAEIRKRREDDEAIPEINIDYELESVQMDDINYHYILTLIQAFVDQEQEALQERLND
                     NPMDQYIQDLAKSNPAMADSLAELWQDIQKEPKAYEGKSIAYELDNLIGDKIQRAIKH
                     FADQWKADPDKLAFVATNYHSANSTKQVGISTLKESLDYQAYKEKQGDSAMNKLKYKS
                     QFERELVQFIRDQIQPLKGH"
     gene            7516..8658
                     /locus_tag="VC394_001480"
     CDS             7516..8658
                     /locus_tag="VC394_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEP5994936.1"
                     /translation="MTINRVLKDDNLLQYTEKEFIEDMKILVAIDSFKGSVTSPELNT
                     SVAQALLSVDKQLVIETRAIADGGEGSLAALSQTVAGRWHQVKTIDLLRRPIKVAYYR
                     HAKQAFIESASIIGIDKITPNSVTYAQATSYGLGLAVKDAIQKGATQIEIMLGGTGTS
                     DGGKGFLESLNYDFMTGRSYLDTLASPVTLLGLTDVTNPYHGPQGFAAVFGPQKGGSL
                     SQIEETDQIASNFAKKVFYQKTIDLQTIPGSGAAGGLGGAIVLLGGTLTSGFSRIAEL
                     LNLDNSLQSCDLVITGEGCLDTQSQSGKVPVAIARMAKKYQVPTIALCGSVKIETGLA
                     AEDFLAVFSIQQQPISLEAAIDKTTTLSNIKILVANLMLLIAQFNK"
BASE COUNT         2635 a         1527 c         1683 g         2925 t
ORIGIN      
        1 cagataatat ggtagtgcca tccccatatg gagatattgt ttataggttt ataaaagagc
       61 taataaacaa tgctataaaa tatggagatg gaaaagacat tcgtttatct ttaacgattc
      121 aatctgatat tattatcatt gaggagaaca atcaagtagt ggaaaaagta cactctataa
      181 gttatggcag aggattgaaa tcatttcaag aaacactagc agcttttgat ggagatctag
      241 aactacaaat ggatactaaa caatttacta tcagaatatt acttcccata gattggaaat
      301 tgtgttatga agattttatt aattgatgac cacagactat ttgcaaagag tatccaactg
      361 ttattccaac agtatgatga agtagatgtc atagatacga ttacttctca ttttaacgat
      421 gtgacgattg atttgtctaa gtacgatatt acattgcttg atattaattt aacaaatatt
      481 tctaaagaaa atggattgga gatagcaaaa gaactcatac agtctactcc tcatttgaaa
      541 gttgttatgt tgacaggata tgttaaatca atctatagag agagggcaaa aaaagtagga
      601 gcatacggtt ttgtagataa aaatatagac cctaaacaac tcatttctat cttaaaaaaa
      661 gtggattctg gaaaaaagta ttttgaacaa atcgaatctc aggattatgt tgaatcttta
      721 actgaccagg aaatagctat cttaaatttg agtaagaaag gattctctat aaaagaaata
      781 gaggaaacgt tacaaattag taggagaaca gtatttaatc atttgaccca catctactca
      841 aagcttttgg taaataataa gcaagaggct atctacaaag ccgaacaatt agggtatttt
      901 atggatttct gagttgcaga taatcaaacg acataagttt tacttgtgtc gttgctaggg
      961 atttcctgtt aaatacccag atttaaactc attgtttttg agttcttttt gattgaaatt
     1021 gattgagata acgcttttat aaaaagtagg gcaatgcctg atatgaaaag ctttttaaat
     1081 ttagtcaaac aaaagttgtt taaaccaggt ctaaaaaaac tcgtaaagct tcacaactcc
     1141 cagaacgtta atatatgctt atatatcaac gattggaact actttatggt tcgcaccatg
     1201 gtttttgtgg aaggatcaaa agttgtcctg aaaatttctc ttaaccgtgt ttaagctttc
     1261 taaacgtttg agaaatcatt gtaacttcaa ataactattt taaataaaac aaagaaacgg
     1321 acaaagttag atgagatgat tctattttga tttataggtg aagaaggaga atcttgtttg
     1381 ataagtactc aataaaggtt cactacccaa ttttaaataa aacgccacag tagtaatcat
     1441 ctactgtggt gttttttggt cttttagttt ctaaaagtat ccaattcgtc ttgtgcttct
     1501 ttggttgttc cgactagttg tttcatgagt tgatctagtt tggcatttgt tttggcaatt
     1561 tcttgatcaa tgtcactgag ctgtttagct aattctggca gtggtttgac ggggacttcc
     1621 tcaaaggtat cgacatagcg tgggatattt aagttatagt cattttcaat gatttcatca
     1681 aatgaggcta aatatgaaaa cttatcagaa ttatcacgac tcttataagc atcaaggatc
     1741 tttttgatat ggctatctgt cattgtgttt tgatttttgc ctttatcaaa ctcttttgaa
     1801 gcatcgataa agaagacatc tttgttggtt ctgttcttct ttaatatgat aatagtcgta
     1861 gggatgcttg tattataaaa gatatttgat gggagtccga taatggtatc gatagcccct
     1921 tgttcgagta gtttttggcg aatcttgcct tctgcagcac cacggaaaag gacaccgtgt
     1981 ggtaagacaa tcgccatagt tcctgtattt tttaaatgat agaaaccatg aagtaaaaag
     2041 gcaaagtcag cttttgattt tggtgctaat acaccataac ttgaaaaccg tggatctgtt
     2101 aagaaaccag cagtcgctga ccatttcaaa gaataaggtg ggttcattaa cactccatca
     2161 aagtttgttg gttcatctgt tggccagtct gcatccaagg tatcagcgtt acttaagtgt
     2221 tggttttcga tggcaacgcc atgaagcatc atgttcatac gagccaagtt ataagtagaa
     2281 gtattgattt cttgaccata gtaagagacg gtatcggatt ggttactata tttcttagca
     2341 tttaaaagga gtgatccaga tcccatagca gggtcataaa gggtcattcc tttttgatct
     2401 tcccaaccta aaaagacaat ctgagtcatc aagtgagaga cagcttgtgg ggtatagaac
     2461 tcacctgctt tttttcctga ctcactggca aattcaccaa tcaagtattc ataagcatca
     2521 cctaaagtat ccccatctac tgcctcaaag tcaatttcat ttaatgtttt catgacatta
     2581 gaaatggttt gattttgttt ttgaggagta gagcctaatt ttttagagta gaggtcaata
     2641 tcttcaaata agttttcaaa gtcttcacca ctttgttcaa tatcgcgaaa accttgagct
     2701 aatgattcta attggaaagt attgtgataa acatcttgga tgagtttttc aaaggtcagt
     2761 gtaggttcga taaagtaacc taaatcacca gtaacaacag aaatgaggtc atcttttagg
     2821 ccttcatctt gataggcatt ttcaaagatt ttttgagcat ctgtaaaggt attaaagtgt
     2881 tcttctaaat tatcacaaac agcaaagagt aatttatcag ataaatgttt gtaaaagatg
     2941 agtccaagaa gatagttttt atagtcattg gcatccattt ggccacgtaa ttgatcggcc
     3001 gaatgccata gtgcttgccg tagtgaagtt gttttctctg ccatgtgcgt cctttcttat
     3061 acgaataaac gattgagtaa tgtttgtttg agtgctttga gttctgttag tttttcttca
     3121 gattgagcaa tttgttggtc taaagtttgg aagaaattac caatggcttc ttgttctgag
     3181 agggaaggga tttgaatttt agcatatttg atatcactag agctaatgct atcaaaagtt
     3241 gaacctgtac taattctttt ccaataacca atttctttca agtattttaa tatttgaaag
     3301 ataaattcat taccttttat agcagcaact ccacgcccaa taataacatg ataatttgtc
     3361 tttccaacat caccaactgg agccctaaca ctaagaataa tatcaccttt gtcagcttgt
     3421 tttgtaattt gtgttgtcca gacacgcgga aaaacatatc cattttttat atcggcattt
     3481 ccttgtacta gaatataatc agaagggttt gtagtataat tttgacttga tggagattgg
     3541 cccattgtaa tttgaacaat atcacctagt tccttctctt cccactcacc atcaaaacct
     3601 tgtaaacgga tttcagggac tttttgtcct tgagctggga acatctttct gaggaaggtt
     3661 tgcttttgtt ctttaaggat tgctagcttt tgcctctgta actgaattaa ctgatcaacc
     3721 gtttggaaga gttcgccaat ggcttcttgt tcagggaggg aggggagatt gataacttga
     3781 tttcgaatta tttcagaatt cagattcact tggcttcctg gttgaccata tttattccaa
     3841 tatggtctga acatatctaa ccactgaaac ataaattcac ggttgaattt aggatctaaa
     3901 aacacaagaa atccatcgtg tactccagtt ttaacataat tgataactgg tttacctact
     3961 gtagctgcaa tacttaatag taaatgtgga tcctttaata ctctagtctt ttcttgacca
     4021 agctcagata ttctttgttg taaataagtt atacgaccct cttgttcagt aacatcagaa
     4081 attcttaacc aacctatatc acttttggca tcaaaccatt ttggatcttg tatcggtcta
     4141 ggactggcac cacgaactat attagaaatt tctcccagct ttttctcttc ccactcccct
     4201 tcaaaactgt caaagcgata ttgaggccgt ttagattttg tcatctcttt cctttcctag
     4261 tgccctttta agggttggat ttgatctcta atgaattgaa ctaactcccg ttcaaattgg
     4321 cttttgtatt tgagtttatt catggccgag tcaccttgtt tttctttata ggcttggtag
     4381 tctagggatt cttttaaggt tgatatgccc acttgtttgg tagagttagc actatgataa
     4441 ttagtcgcca caaaggctaa tttgtcagga tctgccttcc actggtccgc aaagtgtttg
     4501 atggcccttt ggatcttatc accaatgagg ttatccaact cataagcaat gcttttccct
     4561 tcataggctt tgggttcttt ttggatatct tgccataact cagctagtga gtcagccata
     4621 gcagggtttg attttgccaa atcttggatg tattgatcca tgggattgtc attgagcctt
     4681 tcttgaagag cttcttgttc ttgatcgaca aaggcttgaa ttaaggtcaa gatatagtga
     4741 tagttgatat catccatctg aacactttct agctcatagt cgatattgat ctctgggatg
     4801 gcttcgtcat cttctcttcg ctttcgtatt tctgcaatga cattttggta agtgccttta
     4861 taagtatcaa tcacttcatc tgataaccca acctcactca atagtgtttc attaaagtca
     4921 ctatagacct gtacagatgc atagagctta tcaaacgctt gaaaggcttt ggcgtattgt
     4981 ttgagaaaag ctgtctgtgc gctctcaata gttgggaaag cgtctggatc aggaactata
     5041 ttttttaata ctgttacttt ttcaaagaat cttgcttttt cttcttccca agaaggcgct
     5101 aaaacttcac tttgaccacc atttgagtat aggctaagag ccgtatcaac agcttcttta
     5161 aaacgaagtg gtgtttggaa agtcacaacc tgaccgtaat gttttctact ttcaaagata
     5221 cggttggttc gtgagaaagc ttgaataatg tgctgaggtt tcataggctg gcgatcgatg
     5281 aagatggttg ataaacaagg cgcatcaaag cctgttaaga gacggtcaac cacaatcacg
     5341 aggtctaatt gttcatgacg gtctttgaat ttatcttttt tacgggctaa gcgatcattt
     5401 aaatctctgt tatagccttg taagttatcg atggtaaagt tagtgccaaa gaggtgatta
     5461 tagtcttcta gatttttggt cattttatct tgtcggctga tagacgcatt gtcattttca
     5521 gtgatagaat aagtaatagc tacctttgga aagtctggta gtttttcttt gaccttctta
     5581 gaaatgacta aatccgtttc accagcctta actttcttca tcagatcata ataggcttgg
     5641 gcacgagcaa tactcttaac ggttagtaaa ccttcgaatg tctgaccaat tccattatta
     5701 aaaccaagtt tcttgcgtga ttgattaatg atactatcta agacagctaa catatgttct
     5761 tcatgatcat aagcttcttc cggaatactg tcttcaggca tatcaggaat ggttgtctta
     5821 tactcaactt gaaagcctag aaccgcttta tcatgaatgg cttctttaac ggtgtattga
     5881 tggagacatt tgccgtattg ctgttccgtt gtttgggcta aatcacctaa ttgagcacgc
     5941 ttattttcca caaagatagg agtgccggta aagccatacc agcgactgtt acgaaaagta
     6001 ttcgtgagat aacgttgacg ttccggtgtg acagcacggt ggcattcgtc cacaacaaag
     6061 acaacattta agtgtgcaag acgctctttt agtttcttga attttggagt gtcataactt
     6121 tccatctgac tgatcatggc attgagtttt tgaatggtgg tggccacaac acgacgatca
     6181 ctggattcta aatttttgat gagttgtctc gtatcttctg tctcatcgac atcaaagata
     6241 tcattttgcg cataggattg aaaagcagaa gcggtctgat tatctaaatc ctttcggtca
     6301 atgacaaaga tggatttttc aacggccggt atttgaagga tattgcgagc cactttatag
     6361 gaagttaagg tttttccaga acctgttgtg tgccagatat agccagactt gcggtgacga
     6421 ctcgcttcag cgacggcttc gattgcatga atctgataag gacgaaggag aattagtgct
     6481 tttttatcat catcaatgac agagtaagtc atgaccattt ggtgagcacg agggatagat
     6541 aggacttctt tggcaaaggc aaagagatct ttttgtggtt tgttgttttg atctacccat
     6601 tgggtcaaga agttagggtt taatttattt tctttagctg cagcgatata ccttgtatct
     6661 gttttatttg agaccacaaa catttgtaag gttgaaaaga tacctctaaa ctggccttct
     6721 ttatcatact ttttgacttg gttaaaagct tcaatgcatt gatgattctg agattttaat
     6781 tcaatttgga tcataggtaa gccattgatg agaagtgtca catcacctcg tctatcacga
     6841 gaaccagaga aagcaacttg attggcaatc tcataaacag aagttccacc agccacattg
     6901 tctgctttaa cgacttctaa acgaatcgtt cctagttttg catcttcgcg ttgcacttgt
     6961 actttagcaa tgccgttttc accagctaac catttagccg cttcatagta attgacaaag
     7021 ttaagttgat tcttaatctg ctctttttca ctagctgtta aaggctcctc attcagatat
     7081 tgcgtgttat tttgcgctaa gatcttaaag aaattatccc ataaagcatc ttcagttttt
     7141 aattctttac gatatgtcca ttgactttca cctgtttcta agagatggat caactctttt
     7201 tcaagttcaa gttccgtttt agtttgattt cccattttta atccccattc gacatgatat
     7261 agtaatatta tatcattggc ggatcctttt tagaatggca aagctattga aaccttccaa
     7321 acagtctttt tttgattagt tgaatgctta agatcctagc aactaaacaa agctatgact
     7381 atgtaaatgc aatcgagaaa gttcttgtat ttttgagttt gcttgacaat aatttaaggc
     7441 gaagaaagta ataagacaac actagtaaaa aggtcgagtt ctcaaaatag ataaaaaact
     7501 taagacagat ctagcttgac tattaatagg gttttaaagg acgataatct gctacaatat
     7561 acagaaaagg aatttattga agatatgaaa attttggtag ccatagattc ttttaaggga
     7621 tcggtaacgt ctccagaact taacactagt gtagctcaag cccttctttc tgtcgataag
     7681 cagttagtta ttgaaacaag agctatcgct gatggaggag aaggaagttt agcagccctc
     7741 tcacaaaccg tggcggggag gtggcatcaa gtaaaaacca ttgatctatt aagacgcccg
     7801 attaaagtcg cttattatcg tcacgcaaag caagctttta ttgaatctgc gagtattatt
     7861 ggtattgata agatcacccc caattcagta acctatgcac aagccacttc ctatggtttg
     7921 ggtttggcag ttaaagatgc cattcaaaaa ggagctactc aaatcgaaat catgctagga
     7981 ggaacaggca cttctgatgg tggcaaaggg tttttggaaa gtttaaacta tgatttcatg
     8041 acaggaagat cttatcttga tactttagca tcacctgtta cgctattagg gctgactgat
     8101 gtgaccaatc catatcatgg ccctcaagga tttgctgcgg tctttggccc tcaaaaaggt
     8161 gggagtctta gtcaaattga agaaactgat caaattgcaa gtaactttgc taaaaaagtt
     8221 ttttaccaaa aaaccattga tttacagaca ataccaggaa gtggagcagc tggaggatta
     8281 ggaggtgcca ttgtcctatt aggaggcacc ttaactagtg gttttagcag gattgcagag
     8341 ctactaaatc tggataattc ccttcagtcc tgtgatttag ttattactgg agaaggatgc
     8401 ctggatacac aatcacaatc tggcaaggta cctgttgcga ttgcaagaat ggctaaaaaa
     8461 tatcaagttc caacgattgc tctatgtggt tctgtaaaga ttgagacagg actagcagca
     8521 gaagactttt tggctgtttt ttcaatacaa cagcagccaa tttccttaga agcagcgata
     8581 gataaaacaa caacactttc caatatcaaa atattagtag ctaaccttat gctattgata
     8641 gcccagttta ataagtaaac tcatctattt tcgattgtgt agcattcatt tggcggttaa
     8701 aagtaaccta tgaagaagga gacgtacgag ctaactcatt agcatagtat gaaaaacaga
     8761 aacattttgt
//
LOCUS       DATXQK010000054         8015 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.054, whole genome shotgun sequence.
ACCESSION   DATXQK010000054 DATXQK010000000
VERSION     DATXQK010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8015
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.054"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            56..922
                     /locus_tag="VC394_001481"
     CDS             56..922
                     /locus_tag="VC394_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HEP5994937.1"
                     /translation="MKMKKKFFVLSLLVLSTFFLSACSSWIDKGESITAVGSTALQPL
                     VEAVADEFGSSNLGKTVNVQGGGSGTGLSQVQSGAVQIGNSDVFAEEKDGIDASKLVD
                     HQVAVAGLAVIANPKVKVSNLSSQQLQKIFSGEYTNWKQVGGEDLAISVINRAASSGS
                     RATFDSVIMKGVNAKQSQEQDSNGMVKSIVSQTPGAISYLSFAYVDSSVKSLQLNGFK
                     ANAKNVATNDWPIWSYEHMYTKDKPTGLTKEFLDYMFSDEVQQNIVTHMGYISINDME
                     VVKSHDGKVTKR"
     gene            933..1868
                     /gene="pstC"
                     /locus_tag="VC394_001482"
     CDS             933..1868
                     /gene="pstC"
                     /locus_tag="VC394_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEP5994938.1"
                     /translation="MENQELAKKLASPSKNSRLETFGRTITFLCLALIVFIVAMILIF
                     VAQKGLSTFFVDKVNLFDFLFGKEWQPSVKNAAGIPYLGALPMITGSFLVTILSAIIA
                     TPFAIGAAVFMTEISPKYGAKLLQPAVELLVGIPSVVYGFIGLQVIVPFMRSIFGGTG
                     FGILSGVCVLFVMILPTVTFMTTDSLRAVPRHYREASMAMGATCWQTIWRVVLNAARP
                     GIFTAIIFGMARAFGEALAIQMVVGNSAVMPSSLTTPAATLTSVLTMGIGNTVMGTVQ
                     NNVLWSLALVLLLMSLAFNSLVKLITKERKRNYER"
     gene            1858..2745
                     /gene="pstA"
                     /locus_tag="VC394_001483"
     CDS             1858..2745
                     /gene="pstA"
                     /locus_tag="VC394_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEP5994939.1"
                     /translation="MNAKKVDKVATGTLYTIAGIIVAILASLILYILVRGLPHISWSF
                     LTGKSSSYEAGGGIGIQLYNSFFLLIVTLIISIPLSTGAGIYLAEYAKKGPVTNFIRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGSLALTVFNLPQMTRNVEDSLLHVH
                     HTQREAGLALGLSRWETVFYVVIPEALPGMVTGIVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNPLSVTSPISIFRQSETLAVHIWKVNSEGTIPDATLVSAGSAAVLLIFILIF
                     NFSARFIGKKLHSKMTAAK"
     gene            2761..3564
                     /gene="pstB"
                     /locus_tag="VC394_001484"
     CDS             2761..3564
                     /gene="pstB"
                     /locus_tag="VC394_001484"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEP5994940.1"
                     /translation="MTEYNWNERHIITFPEETLALATKDLHVYYGAKEAIKGIDMQFE
                     KHKITALIGPSGCGKSTYLRSLNRMNDTIDIARVTGEILYQGIDVNRKDMNVYEIRKH
                     LGMVFQRPNPFAKSIYKNITFAHERAGVKDKKVLDEIVETSLKQAALWDQVKDDLHKS
                     AFTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNYTIIIV
                     THNMQQAARASDYTAFFYLGNLIEYDKTRNIFQNAQCQSTNDYVSGHFG"
     gene            3577..4335
                     /gene="pstB"
                     /locus_tag="VC394_001485"
     CDS             3577..4335
                     /gene="pstB"
                     /locus_tag="VC394_001485"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEP5994941.1"
                     /translation="MTEPILQIRDLSVYYNQKKTLKDVSLDLYPNEITALIGPSGSGK
                     STLLRSINRMNDLNPEVTITGSIVYNGHNIYSPRTDTVDLRKEIGMVFQQPNPFPMSI
                     YENVVYGLRLKGIRDKSILDHAVESSLKGASIWNEVKDRLHDSAVGLSGGQQQRVCIA
                     RVLATSPRIILLDEPTSALDPISAGKIEETLLLLKKDYTLAIVTRSMQQASRLSDRTG
                     FFLEGDLLECGPTKAMFMNPKRKETEDYISGKFG"
     gene            4403..5056
                     /gene="phoU"
                     /locus_tag="VC394_001486"
     CDS             4403..5056
                     /gene="phoU"
                     /locus_tag="VC394_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEP5994942.1"
                     /translation="MLRTKFEEELDKLHNQFYSMGTEVLAQINKTVRAFVSHDRELAK
                     EVIEEDDTINNFETKLEKKSLEIIALQQPVSNDLRMVITVLKASSDIERMGDHAASIA
                     KATIRMKGEERIPVVEEQINLMGKAVKQMVEEALNAYINADDTKAYEIAASDEIIDQY
                     FRNIQTLAVEEIRKSPDAVFAGKEYFQVLMYLERIGDYARNICEWIVYLKTGKIIEL"
     gene            5261..7798
                     /locus_tag="VC394_001487"
     CDS             5261..7798
                     /locus_tag="VC394_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014612305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEP5994943.1"
                     /translation="MKTVEHLIETFVPENYNIFLDINRQTKTFTGNVAINGEALDNHV
                     AFHQKDLDIKSILLDNEAVIYQVDNDNEVVRVELPETGMMTLVIEFSGSITDNMTGIY
                     PSYYTKNGEKKEVISTQFESHFAREAFPCIDEPQAKATFDLSLTFDQEIGEIALSNMP
                     EVNIDRREETGLWTFDTTPRMSSYLLAFALGELHGKTVESKKGTTVGVYATTAHPLSS
                     LDFSLDIAVRVINFYEDYFGVHYPIPQSLNIALPDFSSGAMENWGLITYREIYLLVDE
                     NSTVQSRQQVALVIAHEIAHQWFGNLVTMKWWDDLWLNESFANMMEYVSIEAIEPSWK
                     IIEDFQTGGIPLALKRDATDGVQSVHVEVNHPDEINTLFDPAIVYAKGSRLMHMLRRF
                     IGDRDFAIGLHHYFEKYQYRNTVGRDLWNILSDTSGKDVAAFMDAWLEQPGYPVLTAR
                     LENDQLILSQKQFFIGKGEEKGRLWPIPLNTNWHGLPETLTEAEMVIPNFSQLAAENE
                     GALRFNIDNTAHYITDYQGSLLDALVTELAQLDNTSALQVIQERRLLADSGLISYAEL
                     VDLIAQLDDSKSYMVAEAVQQVVSGLKRFIDEGSLAEKSFNRLVTTIYQEDFNQHGFE
                     KKADESDEDEMVRQVALGRLWLAENQTIIDGLRTIFEAYQNNIASIPAAVRRLVLANQ
                     MKYFETDSLVDIYFETYVATTDNNLRSDLTVAFSQTKQPTTIRRILVSLKDKDIIKPQ
                     DLSYWYNALLGQSFTQDIIWEWARENWDWIKSALGGDMSFDKFVIYPASNFKTPKHLA
                     EYKSFFEPKLDDMAISRNITMGINEIEARVALITKEKEAVIAALSHY"
BASE COUNT         2427 a         1405 c         1642 g         2541 t
ORIGIN      
        1 gtggcactta gtttctacat atataccctt tatttttacg aataggaaat aaataatgaa
       61 aatgaaaaaa aaattctttg tgttaagtct tttggtccta tcaactttct ttttatccgc
      121 atgttctagc tggattgata aaggtgagtc aataaccgct gtaggatcaa cagcactaca
      181 acccttagta gaagcagtag ctgatgaatt tggaagcagt aatctaggca agactgtcaa
      241 tgttcaaggt ggtggttcag gtacagggtt gtctcaagtt caatcaggag ctgtccaaat
      301 tggaaatagt gatgtctttg cggaagaaaa agatggtatt gatgcttcta aattagttga
      361 tcatcaagta gctgttgcag gacttgcagt tattgccaat cctaaagtca aggtttccaa
      421 tctcagtagt cagcagttgc aaaagatttt ttcaggagaa tataccaatt ggaaacaagt
      481 tggaggagaa gatcttgcga tttcagtgat caaccgagca gcaagttctg gctcacgagc
      541 aacctttgac agtgttatca tgaaaggggt caacgctaaa caaagtcaag agcaagactc
      601 caatgggatg gttaaatcga ttgtttcaca aacaccaggt gccatttctt acctttcctt
      661 tgcctacgtt gattcatctg ttaaatcttt gcaattaaat gggtttaagg caaatgctaa
      721 gaacgtggct acaaatgatt ggccaatctg gtcctacgaa cacatgtata ccaaagataa
      781 accaacaggg ttgaccaagg aatttcttga ttatatgttt tcagatgaag tacaacagaa
      841 cattgttaca catatgggat atatttcgat aaatgatatg gaagtggtca aatctcatga
      901 tggaaaagta acaaaaaggt aatggaggaa ttatggaaaa tcaggaattg gctaaaaaat
      961 tagcctcacc atcaaagaat tcaagacttg aaacctttgg ccgcaccatt acgtttttgt
     1021 gtctggcttt gattgttttt atcgttgcga tgattctgat cttcgttgct caaaaagggt
     1081 tgtcaacctt ctttgttgac aaggttaatc tttttgattt cttgtttgga aaggagtggc
     1141 aaccaagtgt gaaaaatgcg gccggcattc cttatcttgg tgctctgcca atgattacag
     1201 gatccttttt ggttaccatt ttatctgcta ttattgccac cccatttgcc attggcgcag
     1261 ccgtttttat gactgaaatc tcacctaaat atggcgctaa attattacag cctgcggttg
     1321 agcttttggt tggaattcct tcggttgttt atgggtttat cggtttgcaa gtgattgttc
     1381 cttttatgcg ctctatcttt ggtggcacag gttttggaat cctatctggg gtctgtgtct
     1441 tgtttgttat gattttacca acagtgactt ttatgacaac agacagtctg cgggcggtgc
     1501 ctcgtcatta ccgcgaagcg tctatggcta tgggagcaac ctgttggcaa accatttggc
     1561 gtgttgttct taatgcagct cgtccaggta tttttactgc cattattttc gggatggcga
     1621 gagcttttgg tgaagcctta gccattcaaa tggttgtcgg taactctgct gtaatgccaa
     1681 gttcactaac gacaccagcc gcaaccttaa cgtctgtctt gacaatgggt attggtaaca
     1741 cggttatggg aacggtccaa aataatgtgc tttggtccct tgccctagtc ttattattaa
     1801 tgagtctggc cttcaattcc cttgtcaaat taatcacgaa agaaagaaag agaaattatg
     1861 aacgctaaaa aagtcgataa agtagcaact ggtactcttt ataccattgc tggaattatt
     1921 gtagctattt tagcttcctt aattctatat atccttgtcc gtggcttgcc acacatcagc
     1981 tggtcctttt taacaggaaa atcgtcttcg tacgaagctg gtgggggaat tggtatccag
     2041 ttgtataatt ccttcttcct attgattgtt accctgatta tttccattcc tttatcaact
     2101 ggagcgggga tttacttggc tgaatatgcc aaaaaagggc ctgttaccaa ctttattaga
     2161 acctgtattg agattctgtc ttccctacca tctgtggttg tagggctctt tggttacttg
     2221 attttcgttg tgcagtttga atatggcttt tctattattt cagggtctct tgctttgacg
     2281 gtctttaatc ttcctcaaat gacccgtaat gttgaagata gtttactgca tgttcatcat
     2341 acccaaagag aagcaggatt agctttaggc ctgtcacgct gggaaacagt tttttatgtg
     2401 gttatcccag aagctctccc ggggatggta acaggtattg tcttagcttc aggtcgtatt
     2461 tttggtgaag cggcagcgct tatctatact gctggtcaat cagcaccagc tctggattgg
     2521 tcaaattgga atccacttag tgttactagt ccaatctcta ttttccgtca atctgagacc
     2581 cttgctgttc atatttggaa agtcaatagc gaagggacaa ttccagatgc tactcttgta
     2641 tccgcaggaa gtgcagcagt gctattaatt tttatcctta ttttcaactt ttcagctcgc
     2701 tttattggga agaaacttca ttctaaaatg acagcagcga aataagatat tggagaaatc
     2761 atgacagaat ataattggaa tgaacgtcat atcatcactt ttcctgaaga aacgcttgct
     2821 ctagctacca aggatttaca tgtttactat ggtgcgaaag aagctatcaa agggattgat
     2881 atgcagtttg aaaaacataa aataacagcc ttgattggtc catctggttg tggcaaatca
     2941 acttatcttc gtagtcttaa ccgcatgaac gataccattg atattgctcg ggttacaggt
     3001 gagattcttt atcagggcat tgatgttaat cgaaaagaca tgaatgttta cgagattcgc
     3061 aagcacttag gaatggtttt tcaaagaccc aatcctttcg ctaaatccat ttataaaaac
     3121 attacctttg cccatgaaag agctggtgtg aaagacaaaa aagttttaga tgaaattgtt
     3181 gagacttcac tgaaacaagc cgcgctttgg gatcaagtta aagatgacct tcacaaatca
     3241 gccttcacct tatcaggggg tcagcaacaa cgcttgtgta ttgctagagc catttctgtc
     3301 aaacctgata ttctattaat ggatgaacca gcctctgccc ttgacccgat tgcaacgatg
     3361 cagctggaag aaaccatgtt tgaattgaag aagaattaca cgattattat tgtgacccac
     3421 aatatgcaac aagcagctcg tgccagtgat tacactgcct tcttctattt aggcaacctc
     3481 attgaatatg ataagacacg taatattttc caaaatgcgc aatgtcagtc aacaaacgac
     3541 tacgtttcag ggcactttgg ttagaaaggt aatatgatga cagaacctat tttacagatc
     3601 agagaccttt ctgtttacta caatcaaaag aaaacgttaa aagatgtctc tttggacctt
     3661 tatccaaatg aaataacagc tttaattggt ccttctggat ctgggaaatc aacgctcttg
     3721 cgttctatca accgtatgaa tgatttgaat cctgaagtaa ccattacagg ctctatcgtc
     3781 tataacggac acaatattta tagtccaagg acagatacgg ttgatctacg caaagaaatt
     3841 ggtatggtat tccaacaacc caacccattt ccgatgtcta tttatgagaa cgtggtttac
     3901 ggccttcgct taaaaggtat cagagataaa tcaatcttag accatgctgt tgaaagctct
     3961 cttaaagggg cttccatttg gaatgaagtc aaagatcgtt tacatgattc tgcggttggc
     4021 ttatcaggcg gacagcaaca gagagtttgc atagctagag ttcttgctac cagccccaga
     4081 ataatccttt tagatgaacc tacctcagct cttgatccta tctcagctgg gaaaattgaa
     4141 gaaacattat tgttattaaa aaaagattat acactggcta ttgtgacccg ctcaatgcag
     4201 caagcctcac gtttatcaga tcgaacaggt tttttcttgg agggtgactt acttgagtgt
     4261 ggtcctacaa aagctatgtt tatgaatcct aagcgcaaag aaaccgaaga ctatatttct
     4321 ggtaaatttg gttgagaaag acgtatatgg ttgacccctc tgtgttttta ttataatgac
     4381 tagattgtag gaagaaaaaa tcatgttaag aacgaaattt gaagaagaat tagataaatt
     4441 gcataaccag ttttattcaa tgggaacgga agtattggca caaatcaata aaactgttcg
     4501 tgcctttgtt agtcatgatc gtgaattggc caaagaagtt atcgaagaag atgacactat
     4561 taataatttt gagacaaagc ttgaaaagaa atcgctggaa attattgccc tacaacagcc
     4621 agtttcgaat gatcttagaa tggttattac tgttttaaag gcatcaagtg atattgaacg
     4681 tatgggtgac catgctgctt cgattgccaa ggccactatt cgtatgaaag gtgaggagcg
     4741 tattccagtg gttgaagaac aaatcaacct tatgggaaaa gctgtgaaac agatggtaga
     4801 agaagccctt aatgcatata tcaatgctga cgatacaaaa gcttatgaaa tcgcagcttc
     4861 tgatgagatt atcgatcaat atttccgtaa tatccaaacc ttagctgttg aagaaatccg
     4921 taagtctcca gatgctgttt ttgcaggaaa agaatatttt caagtattaa tgtatttgga
     4981 acgtattggt gactatgctc gcaacatttg tgaatggatt gtttatctta aaactggaaa
     5041 aattattgaa ttataagaaa ttatgctata atgagactgg gaaacagctt gccagtccct
     5101 ggttttatac gaaattgtgc aagttagaaa atgaactaac aagaaaagtt ttctttgctg
     5161 aatctgattt tgttaggtta acaaatatat gtagacagag atgatactta ggtatcatct
     5221 gttgatctat tatgatttgt tattagaatt aaggagttat atgaagacag tagaacatct
     5281 catcgaaaca tttgtgcctg aaaattataa catcttttta gatattaatc gtcagactaa
     5341 aacatttacg gggaatgttg cgattaatgg tgaagcttta gataatcatg tggctttcca
     5401 ccaaaaagat ttggacatta agtctatttt gctcgataat gaagctgtta tttatcaagt
     5461 ggataacgac aatgaagttg tgcgtgttga attacctgaa acaggaatga tgacgcttgt
     5521 cattgaattt tcaggtagca ttacagataa tatgacaggt atttaccctt catattacac
     5581 taaaaatggt gagaaaaaag aagtcatttc aactcagttt gaaagccatt ttgcacgtga
     5641 agctttccca tgtattgatg aaccacaagc aaaagctact tttgatttaa gcttgacctt
     5701 tgatcaagaa attggtgaga tagcgttatc gaatatgcca gaagtcaata ttgaccgtcg
     5761 cgaggagact ggtctttgga catttgatac gacaccaaga atgtcatctt acttgctagc
     5821 gtttgcgcta ggagaacttc atggaaagac tgtagaaagc aaaaaaggaa caactgttgg
     5881 tgtgtatgcg acaacagcgc atcctcttag ctcgcttgat ttttcattgg atattgcagt
     5941 tcgtgttatc aacttttatg aagactattt tggagtacat tacccaatcc cgcaatctct
     6001 taatattgcg cttccagatt tttcatctgg tgctatggaa aattggggtt taatcactta
     6061 tcgtgaaatt tatttattag tagatgaaaa ttcgactgtt caaagccgac aacaagttgc
     6121 acttgtaatc gcccacgaga tagcacacca atggtttgga aacttggtta caatgaaatg
     6181 gtgggacgat ctgtggttaa atgagagttt tgccaacatg atggaatatg tctctatcga
     6241 ggctattgag ccgtcttgga aaataataga agatttccaa acaggtggta ttcctcttgc
     6301 tttaaaacgt gatgctactg atggtgttca atcagtgcat gttgaagtca atcatccaga
     6361 tgagataaat acattgtttg atcctgctat tgtctatgca aaaggtagtc gtttgatgca
     6421 tatgttacgc cgttttattg gtgatcgtga ttttgccatt ggcttacatc actactttga
     6481 aaaatatcaa tatcgaaata ctgtcgggcg tgatttatgg aatattttat cagatacttc
     6541 aggcaaagac gtagctgctt ttatggatgc ttggcttgaa caaccaggtt atccagtttt
     6601 aacagctcgc cttgaaaacg atcaattgat tttgagtcaa aaacaatttt ttattggaaa
     6661 aggtgaagaa aaaggtcgcc tttggccgat tcctttaaac actaattggc atggccttcc
     6721 tgaaactttg acagaggcag aaatggtcat tccaaacttt agtcaattgg cagcagaaaa
     6781 tgaaggagct ctacgtttca atattgataa tacagctcat tacattacag actaccaagg
     6841 atcattatta gatgccttag tcactgaact tgcccaactt gataatactt ctgctttaca
     6901 agttattcaa gaacgtcgtt tattggcaga tagtggcttg atttcctatg ctgaattggt
     6961 tgacttaatt gctcaattgg acgattctaa gtcttatatg gtagcagaag cggttcagca
     7021 agtggtctct ggtttgaaac gcttcattga tgaaggaagc ttagcagaaa aatcctttaa
     7081 tcgcttggta accaccatct atcaagaaga ctttaatcag catggttttg agaaaaaagc
     7141 tgacgaatca gatgaagatg agatggtacg tcaggttgcc ttgggccgct tgtggctagc
     7201 agaaaatcag acgattattg acggattgag aactattttt gaagcatatc aaaacaatat
     7261 tgcaagtatt ccagcagctg ttcgtcgctt ggttttagcc aaccaaatga aatattttga
     7321 aacagatagc ttggttgata tttattttga gacttatgta gcaactactg ataataattt
     7381 acgcagtgat ttaacggttg cttttagtca aacaaaacaa ccaactacta ttagacgtat
     7441 tcttgttagt ttaaaagata aagatattat taaacctcag gatttatctt attggtacaa
     7501 tgctttgctt ggacaaagct ttactcaaga tatcatttgg gaatgggcta gagaaaattg
     7561 ggattggatt aaatctgcac ttggtggaga tatgagcttt gataaatttg ttatttatcc
     7621 agcaagtaac tttaaaacac caaaacatct agcagaatat aaatctttct ttgaacctaa
     7681 gctagatgat atggctatta gccgtaatat taccatggga attaatgaaa ttgaagcacg
     7741 tgttgctttg atcactaaag aaaaagaagc agttattgca gctctcagtc attactagac
     7801 cataagcaaa agagttggga ggactttcca gctctttttt gtagtgagct agcttccttt
     7861 cttagttccg tctataacta aatgattatc catagtttaa ttcaaaagga gattgcactt
     7921 taaagcgaag gctattagcc tcttaatact ttttttacca ggtggtgaat aagaacaatt
     7981 ataaatgatt taatcattat gctttaaatg cctta
//
LOCUS       DATXQK010000055         7993 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.055, whole genome shotgun sequence.
ACCESSION   DATXQK010000055 DATXQK010000000
VERSION     DATXQK010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7993
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.055"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(179..1483)
                     /locus_tag="VC394_001488"
     CDS             complement(179..1483)
                     /locus_tag="VC394_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEP5994944.1"
                     /translation="MIISRSRGKTMKIDKRHLLNYSILLPYLILSVIGLIMVYSTTSV
                     SLIQAHANPFKSVINQGVFWIISLVAITFIYKLKLNFLTNTRVLTVVMLGEAFLLIIA
                     RFFTTAIKGAHGWIVIGPVSFQPAEYLKIIMVWYLALTFAKIQKNISLYDYQALTRRK
                     WWPTQWNDLRDWRVYSLLMVLLVAAQPDLGNASIIVLTAIIMFSISGIGYRWFSAILV
                     MITGLSTVFLGTIAVIGVERVAKIPVFGYVAKRFSAFFNPFHDLTDSGHQLANSYYAM
                     SNGGWFGQGLGNSIEKRGYLPEAQTDFVFSVVIEELGLIGAGFILALVFFLILRIMNV
                     GIKAKNPFNAMMALGVGGMMLMQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVA
                     VGFVLNIDASEKRDDIFKEAELSYRKDTRKENSKVVNIKQFQ"
     gene            complement(1623..4421)
                     /gene="ppc"
                     /locus_tag="VC394_001489"
     CDS             complement(1623..4421)
                     /gene="ppc"
                     /locus_tag="VC394_001489"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEP5994945.1"
                     /translation="MIEETSKEGDGHLPLKKLESSNNQAIIAEEVALLKEMLENITRR
                     MIGDDAFAVIESIMVLSEKQDYIELEKVVANISNQEMEVISRYFSILPLLINISEDVD
                     LAYEINYQNNTDTDYLGKLALTIKDLAGKDNGKDILEQVNVVPVLTAHPTQVQRKTIL
                     ELTTHIHKLLRKYRDAKAGVINLEKWRQELYRYIEMIMQTDIIREKKLQVKNEIKNVM
                     QYYDGSLIQAVTKLTTEYKNLAQKHGLELDNPKPITMGMWIGGDRDGNPFVTAETLCL
                     SATVQSEVILNYYIDKLAALYRTFSLSSTLVQPNSEVERLASLSQDQSIYRGNEPYRR
                     AFHYIQSRLKQTQIQLTNQPAARMSSSVGLSTSAWSSPASLENPILAYDSPVDFKADL
                     KAIEQSLLDNGNSALIEGDLREVMQAVDIFGFFLASIDMRQDSSVQESCVAELLKGAN
                     IVDDYSSLSETEKCDVLLQQLMEEPRTLSSAAVAKSDLLEKELAIYTTARELKDKLGE
                     EVIKQHIISHTESVSDMFELAIMLKEVGLVDQQRARVQIVPLFETIEDLDNARDIMAA
                     YLSHDIVKSWIATNRNYQEIMLGYSDSNKDGGYLASGWTLYKAQNELTAIGEEHGVKI
                     TFFHGRGGTVGRGGGPSYDAITSQPFGSIKDRIRLTEQGEIIENKYGNKDVAYYHLEM
                     LISASINRMVTQMITDPNEIDSFREIMDSIVADSNTIYRKLVFDNPHFYDYFFEASPI
                     KEVSSLNIGSRPAARKTITEITGLRAIPWVFSWSQNRIMFPGWYGVGSAFKRYIDRAQ
                     GNLERLQHMYQTWPFFHSLLSNVDMVLSKSNMNIAFQYAQLAESQDVRDVFYEILDEW
                     QLTKNVILAIQDHDDLLEDNPSLKHSLKSRLPYFNVLNYIQIELIKRWRNNQLDENDE
                     KLIHTTINGIATGLRNSG"
     gene            4580..6379
                     /gene="pepF"
                     /locus_tag="VC394_001490"
     CDS             4580..6379
                     /gene="pepF"
                     /locus_tag="VC394_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012766712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEP5994946.1"
                     /translation="MELKKRSEFPENELWDLTALYKDRQDFLLAIEKALQDIDLFKRN
                     YEGRLTSVDDFTQALIEIEHIYIQMSHIGTYAFMPQTTDFSDESFAQIAQAGDDFMTK
                     ASVALSFFDTALANADLDVLDTLEKNPYFSAAIRMAKIQKEHLLSPDVEKALANLREV
                     INAPYDIYTKMRAGDFDMEDFEVDGKTYKNSFVSYENFYQNHENAEIRDKAFRSFSKG
                     LRKHQNTAAAAYLAKVKSEKLLADMKGYASVFDYLLAEQEVDRSLFDRQIDLIMTEFG
                     PVAQKFLKHVAQVNGLEKMTFADWKLDIDNDLNPEVSIDGAYDLVMKSLAPLGQEYTK
                     EIERYQTERWVDFAANANKDSGGYAADPYKVHPYVLMSWTGRMSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNTHMSTYYVEAPSTFNELMLSDYLEHQFDDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGGTFGADQLNAMMKEVLTDFWGDAVDIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLNLKNNPDGAKEWLDFLKSGGSRTPLDTAMLIG
                     ADIATEKPLRDTIQFLSDTVDQIINYTEEMSHA"
     gene            6372..6851
                     /locus_tag="VC394_001491"
     CDS             6372..6851
                     /locus_tag="VC394_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEP5994947.1"
                     /translation="MPNLYDFTVKAQNGNDLSLAAYKKKVVLIVNTATKCGLTPQYQA
                     LQALYDTYHDKGFEVLDFPCNQFLNQAPGDAEEINHFCSLTYHTTFPRFAKIKVNGKD
                     ADPLFTWLKEEKSGPLGKRIEWNFTKFLIDQNGQVIKRYSSKTDPKLIEEDLKALLG"
     gene            complement(6898..7281)
                     /locus_tag="VC394_001492"
     CDS             complement(6898..7281)
                     /locus_tag="VC394_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEP5994948.1"
                     /translation="MNKRFKTGFLALVAMLLVACSQGTKQTQTPSVPKADHHVRLVVK
                     EDTNTIDEKVSFGKGDTVLEILKDNYEVKEKDGFITAIDGIEQDTKANKYWLFKVNGK
                     MADKGADQITVKDGDSIEFYQEVFK"
     gene            complement(7265..7768)
                     /locus_tag="VC394_001493"
     CDS             complement(7265..7768)
                     /locus_tag="VC394_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEP5994949.1"
                     /translation="MSAKFMSRIAIMSALCVVLRMVFSSLPNVQPVTAFLLSYLLYFG
                     LAEAVLVMMLCLFLSAFLLGFGPWVFWQVTCFVLVLLLWRFALYPLSQQFPKYQLGYQ
                     AFLAALCGLLYGVLIDTCFAYLYSMPWWSYVLAGMPFNIAHALSTLVFFPVVMMLFRR
                     LIGEQKI"
BASE COUNT         2559 a         1673 c         1411 g         2350 t
ORIGIN      
        1 tacttatagc agcgacttcc tttggaattc cacaccttat ttttgccaca gcaaaaagca
       61 gctgtttggg ctcatgtcac acgcaaaaat aagaaatcag cagagcagtt caagtttttt
      121 gagcatcaaa ctgggattgt ctatattcta aaatctcctt aaaggagatt ttagaatatt
      181 attgaaattg cttgatattt acaactttac tattttcttt tcgagtgtcc tttcgataag
      241 ataattcagc ttctttaaat atatcatctc gcttttcgct agcatcaata tttagtacaa
      301 aacccactgc aacagataag acaagtaagc tatttccacc ttgagacaaa aaagggaagg
      361 taacaccagt agatggaatt aagcctgaga taccaccaat attaacaaag acttgcatga
      421 gcatcatacc gcctactcct aaagccatca tggcattaaa aggatttttt gctttaatac
      481 caacattcat aatacgtaaa attaagaaga aaacaagtgc caaaataaac cctgctccta
      541 ttagacctaa ctcttcaatg acaactgaaa agacaaagtc tgtctgtgct tcgggcaaat
      601 agccacgctt ttcaatggaa tttcctaacc cttggccaaa ccacccacca ttactcatag
      661 catagtaaga attggctaat tggtgacctg aatcggtcaa gtcatgaaaa ggattaaaaa
      721 aggcactaaa acgtttagcg acatagccaa aaacaggaat cttagcaact ctttctaccc
      781 caataacggc aatagtccca agaaagaccg tagatagacc ggtaatcatg actaggatag
      841 ccgaaaacca tcggtaacca atgccactga tagaaaacat aataatggct gtcaatacaa
      901 taatggaagc atttcctaag tcaggctgag cagcaaccaa aagtaccatc aacaaagaat
      961 aaacacgcca gtcccgtaaa tcgttccatt gtgttggcca ccattttcga cgagttagtg
     1021 cttgataatc ataaaggcta atgttttttt ggatcttagc aaaggtcaat gccaaatacc
     1081 ataccataat gatctttaaa tactcagcag gctgaaagct gactggtccg atcacaatcc
     1141 aaccatgcgc tcctttgata gcagttgtaa agaagcgggc aataattaaa agaaaggcct
     1201 ctcctaacat aaccacagtt aatacccttg tattcgttaa aaaatttaat tttaatttat
     1261 agataaatgt aatagccact agacttatta tccaaaaaac tccctggtta atcaccgatt
     1321 taaatggatt agcatgtgct tgaatcaagc tgacacttgt ggttgagtaa accattatta
     1381 agcctatcac cgataaaatt aaataaggca ataatataga ataatttaag aggtgccttt
     1441 tatcaatctt catagttttc cctctacttc ttgagataat catttagcat tataacatct
     1501 ttctatatca aacaaaagaa aaagtcaaga atcctcaaaa aaagaagctc caagggctaa
     1561 cctttaaaat tctgattagt cacttgaaaa cttctataaa taccaattaa tactcaagag
     1621 cattagcctg aattacgtaa accagtcgct atgccattga tagttgtatg gatcaatttt
     1681 tcatcatttt cgtcaagttg attattacgc caacgtttaa tcaattcaat ttgaatatag
     1741 ttcaacacat taaaatacgg caagcgtgac ttcaagctat gcttaaggga aggattatcc
     1801 tccaatagat catcatggtc ttggatagca agaataacat tttttgtcaa ttgccactca
     1861 tccaaaattt cataaaaaac atctcgaaca tcctgacttt cagctagttg tgcatactga
     1921 aaggcaatat tcatgtttga tttagataat accatatcaa catttgataa caaggaatgg
     1981 aaaaaaggcc acgtctgata catgtgttgg aggcgttcaa ggttaccttg tgctcggtca
     2041 atataacgtt taaaggctga acctacccca taccaacctg ggaacatgat gcgattttgt
     2101 gaccatgaaa agacccaagg aatggcacgc aagcctgtaa tttccgtaat cgttttacgg
     2161 gctgctggtc ttgacccaat gttgagactg gaaacttcct taatcgggct tgcctcaaag
     2221 aaataatcat agaaatgggg attgtcaaaa accaattttc gataaatggt attgctatca
     2281 gcaacaatgc tatccatgat ttctcgaaaa ctatcaatct cattaggatc tgttatcatt
     2341 tgagtcacca tacgattgat agaagctgaa atcaacattt ctaaatggta ataagccaca
     2401 tctttattgc cgtatttgtt ttcaataatt tccccttgct ctgtcagacg aatccgatct
     2461 ttgatggaac caaaaggttg tgaagtaatg gcatcataag aagggccacc tcctcgacca
     2521 actgttcctc cacgaccatg gaagaaggtg attttcactc catgttcctc accaatagcc
     2581 gttaattcat tctgagcttt ataaagagtc caacccgatg ccaagtagcc accatcttta
     2641 ttactgtcag aatagcctaa catgatttct tggtaattac gattcgttgc aatccaagat
     2701 ttgacaatgt catggcttaa ataggctgcc atgatgtcac gggcattatc taaatcctca
     2761 atggtttcaa agaggggaac aatttgcaca cgagcccgct gctggtcaac caatcccact
     2821 tctttaagca taatggccaa ctcaaacata tcagagacac tttctgtatg tgaaataata
     2881 tgttgcttta taacctcttc tccaagctta tctttcaatt ctcgtgccgt agtatagata
     2941 gccagttctt tttctaatag gtctgattta gcaactgccg ctgaggataa agtccttggc
     3001 tcctccatta attgttgtaa gagaacatcg catttttcag tttctgaaag agagctataa
     3061 tcgtcaacaa tattggctcc ttttaataac tctgccacac aagattcttg gacactggag
     3121 tcttgcctca tgtcaatgct tgctaagaag aagccaaaaa tatcaacagc ttgcatgact
     3181 tctcgcaaat ccccctcaat caaagcggag ttgccattat caagtagcga ctgctcaata
     3241 gcctttagat cagctttaaa atcaacagga gagtcatagg ctaagatggg attttctaaa
     3301 ctagctggtg aagaccaagc agatgtgctt agtcccacac tactactcat acgagctgct
     3361 ggctgattag ttagttgaat ttgtgtttgt ttaagacgcg attgaatata atgaaaagct
     3421 ctgcgataag gttcatttcc acggtaaatc gattggtcct gagacaaact ggctaaacgt
     3481 tccacttccg agttgggttg taccaaggtc gacgacagtg aaaatgttcg gtaaagggct
     3541 gctaatttgt caatgtagta attgaggata acctcacttt gaaccgtagc tgacaagcac
     3601 aaggtttcag ctgtgacaaa ggggttccca tcacggtccc caccaatcca catccccatg
     3661 gtaatgggtt tgggattgtc taactctaga ccgtgttttt gggctaagtt tttgtactct
     3721 gttgtcaact tggtcactgc ttgaatgaga gaaccatcgt aatactgcat gacatttttt
     3781 atctcgtttt tcacctgaag ctttttctcg cgaataatat ctgtctgcat gatcatttca
     3841 atataacgat atagctcctg acgccatttc tcaagattaa tcacaccagc cttagcatca
     3901 cggtatttgc gcagaagttt atgaatgtga gttgtcaatt ccagaatcgt tttacgttgt
     3961 acttgcgtcg gatgtgctgt taaaacagga accacattaa cctgctccaa aatgtcttta
     4021 ccattatctt ttccagcaag atccttgatg gttagggcta attttcctaa ataatcagta
     4081 tccgtattat tttgataatt gatttcatag gctaaatcga catcttctga aatattgatt
     4141 agcaagggta aaatagaaaa atacctggaa atcacttcca tttcttgatt tgaaatattg
     4201 gcgacaactt tttctaattc tatgtaatct tgtttttcag ataacaccat aatagactcg
     4261 ataactgcaa acgcatcatc tccaatcatc cgtcgtgtga tattttccaa catttctttc
     4321 aaaagagcaa cttcttcagc aatgatagct tgattgttac tactctctaa ctttttaagt
     4381 ggcaaatgac catctccttc tttgctagtt tcttctatca taccactttt taccaaaaat
     4441 aaaagtgatc atgttaaaaa cgtttgtttt ttaacaaaag taagcgcttt attttaaaga
     4501 atgtttggat aggtttagtc tccatattcc actaaaattc tgttatacta gtcttgacta
     4561 tattgttaag gagacaacta tggaattgaa aaaacgttca gaattccctg aaaatgaact
     4621 ttgggatcta acagcgctct acaaagaccg ccaagatttt ttactcgcta ttgaaaaagc
     4681 cttacaagat attgacctgt ttaagcgcaa ttatgaaggc cgcttaacgt cggtggacga
     4741 tttcactcag gccttaatag aaattgagca tatttatatt caaatgagtc atattggcac
     4801 ttatgccttt atgcctcaaa ccactgactt ttcagatgaa agttttgctc aaattgctca
     4861 ggctggcgat gatttcatga ctaaagctag cgttgccctt agcttctttg acaccgcctt
     4921 agccaatgcc gaccttgacg tgttagatac attagaaaag aacccttatt tcagcgcagc
     4981 tattcgcatg gcaaaaattc aaaaagaaca cctcttaagt cctgatgtgg aaaaggcttt
     5041 ggctaactta cgcgaggtta tcaatgcgcc ctatgatatt tacaccaaaa tgcgggctgg
     5101 tgactttgac atggaggatt ttgaggtgga tggtaaaacc tataaaaata gctttgtttc
     5161 ttacgaaaat ttctatcaaa atcacgaaaa tgctgagatt cgtgacaaag ccttccgctc
     5221 tttttcaaaa ggtctccgta aacaccaaaa tacagctgca gcggcttact tagccaaggt
     5281 taaatctgaa aaattgttag cggatatgaa aggctacgct tctgtctttg attacctcct
     5341 agctgaacag gaagtggatc gtagcttatt cgaccgccaa attgacctca tcatgactga
     5401 atttggacca gttgctcaga aattcttaaa acatgtcgcc caagtcaatg gtcttgagaa
     5461 aatgactttt gctgactgga agctagatat tgacaatgat ctcaatccag aggtcagcat
     5521 tgatggtgcc tatgacctcg tgatgaaatc tctagcccca ttagggcaag aatacacgaa
     5581 agaaatcgaa cgttatcaga cagaacgctg ggttgacttt gctgctaacg ccaataagga
     5641 ttctggtggc tatgccgctg atccctacaa ggttcatccg tatgtcttaa tgagttggac
     5701 tggaagaatg tctgatgttt atacccttat tcatgaaatc ggtcactctg gtcaattcat
     5761 tttttcagat aatcaccaaa gttacttcaa cacccatatg tcaacttatt acgtcgaagc
     5821 gccatcgacc tttaatgaat tgatgcttag tgattacttg gaacatcaat ttgatgaccc
     5881 tcgtcaaaaa cgttttgccc ttgctcaccg tttaacagac acttactttc ataacttcat
     5941 cactcaccta ttggaagcgg ctttccaacg taaggtttac accctgattg aagaaggcgg
     6001 tacctttgga gctgaccaat taaatgcgat gatgaaagag gtcttaacag acttctgggg
     6061 agatgccgtt gacatcgacg atgacgcagc tcttacctgg atgcgccaag cgcattacta
     6121 catgggctta tatagttata cctattctgc tgggcttgtt atttcaacag ctggttacct
     6181 taaccttaaa aacaacccag atggggcaaa agaatggctt gacttcctaa aatcaggtgg
     6241 cagccgtacg cctctcgata ctgcaatgct cattggggca gacattgcca ccgaaaagcc
     6301 tcttcgagat accattcaat tcttgagtga taccgttgac caaattatca attacaccga
     6361 ggagatgtca catgcctaat ctctatgatt ttacggttaa ggctcaaaat ggaaatgacc
     6421 tttccttagc tgcttataaa aaaaaggttg ttttgattgt taacacagct accaagtgtg
     6481 gattaacccc acaataccag gccctgcaag cactatatga cacataccat gacaagggat
     6541 ttgaggtctt agattttcct tgcaaccaat ttttaaatca agctccagga gatgctgagg
     6601 agattaatca cttttgcagt ctgacctatc acactacttt ccctcgcttt gctaaaatca
     6661 aagtgaatgg taaagacgct gatcctttgt tcacctggtt aaaagaagaa aaaagtggtc
     6721 cccttgggaa acgcatcgaa tggaatttca ctaaatttct cattgaccaa aatggtcaag
     6781 tcattaaacg ctattcctca aagacagatc ctaaactaat tgaagaagac cttaaagcac
     6841 ttcttggtta atataaaata gacttggcgt gccaagtcta ttttgtatta atagttacta
     6901 tttaaaaact tcttgataaa attcaatgct atcaccatct ttaacggtaa tttgatcggc
     6961 acccttatct gccatctttc cattcacctt aaaaagccag tatttgttag ctttcgtgtc
     7021 ttgctcaata ccatcaattg ctgtgataaa gccatctttt tctttgacct cataattatc
     7081 tttgagaatc tcaagcaccg tatctccttt tccaaaagaa actttttcat caatggtatt
     7141 ggtatcttct ttgacaacta agcgaacgtg atggtctgct tttggaacgc ttggagtctg
     7201 tgtttgtttt gttccttgcg agcaagcaac aagtagcata gcaacaagcg ctagaaatcc
     7261 tgttttaaat cttttgttca ccgattaacc tcctaaataa catcatcaca actggaaaaa
     7321 agacgagagt tgataaagca tgcgctatat taaagggcat ccctgctaaa acataactcc
     7381 accaaggcat actatacaaa tacgcaaaac aagtatctat caacacccca taaaggaggc
     7441 cacataaggc ggccaagaaa gcctgataac caagctgata tttggggaat tgctgagaca
     7501 aagggtacaa ggcaaaacgc cataataaaa gaacaagcac aaaacaagta acttgccaaa
     7561 agacccaagg gccaaatccc agcaaaaagg ctgataaaaa caagcacaac atcataacta
     7621 atacagcctc ggctaaccca aagtaaagaa gataactgag taaaaatgcc gtcacaggct
     7681 gcacattagg caggctgcta aaaaccattc tcaacacaac acatagggct gacatgatag
     7741 caatgcgact cataaactta gctgacataa acttcttcct cgtttttttc tctattatga
     7801 cagatttaca gagcgtttac cagtttgaag ctcacttatt tatcaaatga taataacatg
     7861 agaattaaat aacaaaacag tacttgctga ctgtcatgat atagtcaaag actaaactaa
     7921 tattgtaaag aatatagcta ccaactataa gagatagtga cataaacttg acatagttag
     7981 agaggatctc att
//
LOCUS       DATXQK010000056         7866 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.056, whole genome shotgun sequence.
ACCESSION   DATXQK010000056 DATXQK010000000
VERSION     DATXQK010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7866
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.056"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            656..1267
                     /gene="rpsD"
                     /locus_tag="VC394_001494"
     CDS             656..1267
                     /gene="rpsD"
                     /locus_tag="VC394_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEP5994950.1"
                     /translation="MSRYTGPSWKQSRRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFSYGLGEKQFRNLFVQATKIKEGTLGFNFMVLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVDPGQVISVREKSMKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(1424..2587)
                     /locus_tag="VC394_001495"
     CDS             complement(1424..2587)
                     /locus_tag="VC394_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEP5994951.1"
                     /translation="MNIKEYKKKNGTIIYRTSVYLGVDQVTGKKARTTITASTKKGVK
                     IKARDALNNFAMNGYTVKEKPTVTTYKELTALWWESYKNTIKPNSRQSMEGIVRLHIL
                     PAFGDCKLSKLTTPVIQQQVNKWANNANKGIKGAYANYSFLNNINRRILQYGVTMQVI
                     EHNPARDVIIPRKQNNKEHKVKFFSNQELKQFLNYLDDLDLSSYENFFDYVLYKTLLA
                     TGCRIGEVLALEWSDIDLKKGTIKVSKTLNRYQETNTPKSKAGLRDIEIDRATVLLLK
                     QYKNRQQVLSWDLGRSETIVFTPFTTKYAYACLLRKRLQKHFKAAGVPDISFHGFRHT
                     HATIMLYAGIEAKDLQYRLGHSNISMTLNTYVHATKEGAKKAVSIFETAISNL"
     gene            complement(2767..3210)
                     /locus_tag="VC394_001496"
     CDS             complement(2767..3210)
                     /locus_tag="VC394_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP5994952.1"
                     /translation="MENRLQKIRKEQTNLNQKDFFDQVVNKKLNLDISFRTYQNWENP
                     KNTIKPDKAQILADYFGVWLPYLLGNSNIRTEEQANFLAEMNKRINSEGDSFSFDMTA
                     TESDEHDLLYNYRQLETPYKNQVRKFTRDMWELQKESKNFSPKSP"
     gene            3379..3567
                     /locus_tag="VC394_001497"
     CDS             3379..3567
                     /locus_tag="VC394_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP5994953.1"
                     /translation="MLITENIAVKVRKKRAVKQLGKVELSKKLGITPPTLSKIEAGNY
                     KAPKRIYASVMEWLAEDY"
     gene            3583..4149
                     /locus_tag="VC394_001498"
     CDS             3583..4149
                     /locus_tag="VC394_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
                     /protein_id="HEP5994954.1"
                     /translation="MNRQVSIFEEVNDQGGAFQVVTTMTFHDRLLDIYGNIQEPLFRA
                     RDVAEMIDHSKTRNGYYDVQSMLRKVDDDEKVKGVPLEGATKNFRSGQKVWFLTEHGL
                     YEVLMRSSKPKAKEFRKAVKNILKEIRLNGYYMQGELVQNSQPTPDIDDLSYIKQKLT
                     DLQDVDSLQDLRWKMAKLYHVIETLDEY"
     gene            4206..4448
                     /locus_tag="VC394_001499"
     CDS             4206..4448
                     /locus_tag="VC394_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor protein"
                     /protein_id="HEP5994955.1"
                     /translation="MKATTYKELKKWIDEGVDLAELAQGYADKVPSVDREQFEAVTQG
                     IFNVLEGVSLMLDDKVLIYNRKAEQKRLNDIEQGDY"
     gene            4669..4851
                     /locus_tag="VC394_001500"
     CDS             4669..4851
                     /locus_tag="VC394_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994956.1"
                     /translation="MTSVRTAQLLNNGLGLVGQDLKDVLSALANSVNPSIEKQISHIM
                     IDRANTGEWLANVGKK"
     gene            4863..5195
                     /locus_tag="VC394_001501"
     CDS             4863..5195
                     /locus_tag="VC394_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994957.1"
                     /translation="MKYRVETNPFSKDRYTPEQLEMFKNRQLSKNKAEAYFTRLYNQH
                     IAWVIIANVMTEYVNKFRKSATSFEKAWDALGYQQTTEIVFRAVNGLPCSEKDTGELE
                     TYLSEVSA"
     gene            5195..5386
                     /locus_tag="VC394_001502"
     CDS             5195..5386
                     /locus_tag="VC394_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994958.1"
                     /translation="MQELNLTPTQTLILFIVLGLVGLLLSLSKPLIEIDLPEDTQVPK
                     PPQNANYGAYIQSQNHYYN"
     gene            5295..5663
                     /locus_tag="VC394_001503"
     CDS             5295..5663
                     /locus_tag="VC394_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994959.1"
                     /translation="MTYQKIPKYLNHLRTQTMGLIFNHRTIITIRREQMQLLSREAEL
                     ELLEKVGCYLEKRWQLELENKDDWDLIPRQDLLEKLDITGTTLKNWEKEGLKSYQSPF
                     ENSKKVYYRKSDIYNFLSVD"
     gene            5673..6194
                     /locus_tag="VC394_001504"
     CDS             5673..6194
                     /locus_tag="VC394_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994960.1"
                     /translation="MDYLEVTMTKDCVKIFNFLYKYPTKAYQKDEYFKLLYMHPLDSF
                     LTSFSLSGIRVKVTDKPVLAGWKLVRDIEVRIATGELLEMIEELEICYLRKHQTVSYV
                     EIKFYVVHLLTYGIRSRYDMQFFTKLLFCCGYDQETVIGIYSNITKNTRLSRDFITLQ
                     AKLYQTKKGTHEH"
     gene            6184..7797
                     /locus_tag="VC394_001505"
     CDS             6184..7797
                     /locus_tag="VC394_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage resistance protein"
                     /protein_id="HEP5994961.1"
                     /translation="MSIKENIDKLVSMEWTESIFGQSKMKADYKRQLSKVIELDTLSN
                     IHHNGETYAVSNQEVMYLSESKGRTHKFISGNVKTKSKGKGRDSQKYLVVSFDHQFPP
                     FAKLVVDYLLGRFTFFNNKLYDVNNRQAQVLDDFTIQSLYGFKKDNPFILEILEGIHQ
                     TLNIRPAKQLEAYKISGRDFIIDLKEHRLYRTNPSSDSSFFKFYDVDYHTAIGSKKKV
                     GKFLDYVIADHDSLHNATLQAYYIAQVACGLKSKQNFFISKSGVRTGKGLRHIGLSGI
                     FNKIDVELDLLIKGGFEASNAWGAFAGGEMALATEQGDIVGDKMERVLKIIATEKTHM
                     ARSIGGNLGTVNLTSVLCIDTNRTVALSDEMNGRAVLIQYQNRPEGETDLEREKAFEE
                     YWQTFTEMDKSPKIEGVIGFLLVSLDYFRKNDMKFDWRAVEVVNDDDLDDFQVYLINH
                     LSEKDFVERDSYVRELYNSTYGKNNNQAGQRMRTIGVKSYRKQTTGGRKIPGYKVDNI
                     KRFNKFSGGEKENTNIFDMFEKLNQPLPP"
BASE COUNT         2616 a         1303 c         1577 g         2370 t
ORIGIN      
        1 attcttttta ttttgaaaaa attcctataa aagataatat ttttaggtta gattgaagga
       61 aaaaagaggt cttcaccatg gttaagcaaa tgatcttagt actaaggtta aaagattatc
      121 gtgaaataaa gttttgacac aagtgtaatt aatagaggaa cttgaagtat ccggtaaaac
      181 tatcttgaag tgaaaaaacg agtagacctc ccctgataaa tacaatatac tagccatttc
      241 agagcaatta agagtttcca gtgaagtgct tttgatagag agctaagaaa aaaacagtca
      301 aggtttaaga actgcaaatt agcatttaaa ttattttttg ttaatgacag ttgtctttat
      361 taccataact ataacttctt ataatatagc cttcttaatt catcagtggt ggttcgtccc
      421 atatctgacc ttaaaatatt aaactgatca tcagatgata gatagactga ctttacaaaa
      481 caactttctt atggtaaact gttctttgtg cgaaataaca gcagaaaaat atgaagctcg
      541 tcaacaggtg gttatctatt caagtaacct ttgctgttta ggcgaaaacc ccctgcacga
      601 atcaggattt tttaaaccgt tatttcctca aaaaatttag aggaggacat gtaatatgtc
      661 acgttatact ggtccatcat ggaaacaatc acgtcgcctt ggtttatcac ttacaggcac
      721 aggtaaagaa ttggcacgtc gtaactacgt tcctggtcaa cacggtccaa acaaccgtag
      781 caaactctca gaatatggtt tgcagttagc tgagaaacaa aaacttcgtt tctcatacgg
      841 tttaggtgaa aaacaattcc gtaacttgtt cgttcaagct acaaaaatca aagaaggaac
      901 tcttggtttc aactttatgg ttcttttaga acgtcgtctt gacaacgttg tttaccgtct
      961 aggtttagca actactcgtc gtcaagcacg tcaattcgtt aaccacggtc acatccttgt
     1021 tgacggtaaa cgcgttgata tcccttcata ccgtgttgat ccaggtcaag tgatttcagt
     1081 tcgcgaaaaa tcaatgaaag tacctgcaat ccttgaagct gttgaagcta ctcttggacg
     1141 tccagctttc gtatcatttg atgctgaaaa acttgaaggt tcattgactc gccttccaga
     1201 acgcgatgaa attaacccag aaatcaatga agcacttgtc gttgaattct ataacaaaat
     1261 gttgtaatat tttattgaat aggataggct taaaagcctt gatactaagc actttggggc
     1321 attattccaa ggtgcttttt ttgattttac taccctttta gttctcttta gtcggatttt
     1381 agtatagtaa gagggtagcc ccaaagtggg gcacccttgt taattataaa ttgctaatag
     1441 ctgtctcaaa aatcgagacg gcttttttag cgccttcttt tgttgcatgg acataagtat
     1501 tcaatgtcat tgagatatta gagtgtccta gtctatattg caagtcttta gcctctatcc
     1561 cagcgtaaag cataattgta gcgtgggtgt ggcggaagcc gtggaaacta atatcaggca
     1621 cgccagcagc tttaaaatgc ttttgaagcc tttttctaag caaacaagcg taggcgtatt
     1681 ttgtggtaaa cggagtaaaa acaatcgtct cagaccgtcc tagatcccaa gataaaactt
     1741 gctgacggtt tttatattgc ttaagaagta agacggtagc tctatcaatc tcaatgtctc
     1801 ttaatcctgc ttttgattta ggagtgtttg tctcctgata tctattcaaa gttttggaaa
     1861 ccttgatagt tccttttttt agatcaatat cagaccattc aagagctaga acttctccaa
     1921 tccgacaacc agtagcaagc aaggtcttat aaagaacgta atcaaagaag ttttcatagc
     1981 tagataaatc taagtcatct aaatagttca agaattgctt tagttcttga ttgctgaaga
     2041 actttacctt atgttctttg ttattttgtt ttcgaggaat gatgacgtca cgagctggat
     2101 tatgttcaat aacttgcata gtaacgccat attgtagaat acggcgattg atattattta
     2161 gaaaactgta gttagcatat gcgcctttaa tgcccttatt agcgttgtta gcccacttgt
     2221 taacttgttg ctgaataaca ggggtagtta gcttagatag cttgcaatcc ccaaatgcgg
     2281 gcaaaatatg aagtcttaca atcccttcca tagattgccg agagtttggt ttgatcgtat
     2341 tcttgtaact ctcccaccaa agggctgtta gctctttata agtggttact gtcggcttct
     2401 ctttgacggt atagccattc atagcaaagt tatttagagc atcacgagct tttattttaa
     2461 cccctttttt agttgaagct gtaatagttg tgcgagcctt cttacctgtt acctggtcga
     2521 caccaagata gacactagtg cggtaaatga ttgtaccgtt cttctttttg tattctttta
     2581 tgttcatgat aatacctttc taacatcagt aagcaagtat gggatttagt taggtattta
     2641 ggttttatca tgggtgatag ttggttatct ggtagggtgg cgataaggta gagggtgtgt
     2701 taatgctatg gttgtcaata taagtgttga ttattgtttg gtgggatagt tgcgagctgg
     2761 tcggtatcag ggagattttg gagagaagtt tttgctttct ttttgcaact cccacatgtc
     2821 tctagtaaat tttctaactt gatttttata gggagtttca agctgtctgt aattatataa
     2881 taggtcgtgt tcatcagatt cggttgcggt catgtcgaag gaaaatgaat caccttcaga
     2941 gttaattcgc ttgttcattt ctgctaaaaa attcgcttgc tcttctgttc ggatattgct
     3001 atttcctaga agataaggga gccatactcc aaaatagtcc gccagtattt gagctttatc
     3061 aggcttgatt gtattctttg ggttttccca attttgatat gttctaaatg aaatgtctag
     3121 atttaatttc ttatttacga cttgatcaaa aaaatctttt tgattgagat ttgtttgttc
     3181 ttttcttatt ttttgtagcc tattttccaa aagtattctc ctttatttta aaaacattat
     3241 accattatcc attaaaacaa aaaagcataa gacgaaaaaa aattggattt ttatcttgac
     3301 aaccaaattt atttgcgtta atatagattt tgcaaaaata agacgaaaaa aaattgggtt
     3361 tttgcaaagg aggaatatat gcttattaca gaaaatatag ctgttaaggt tcgtaaaaaa
     3421 agagctgtta aacagttagg aaaagtggag ctttctaaaa aattagggat aacgccaccc
     3481 actctatcaa aaattgaagc tggcaactac aaagcaccta agcgtatcta cgctagtgtc
     3541 atggaatggc tagcagagga ttattagaaa ggcaggaaca aaatgaatag acaggtcagt
     3601 atttttgaag aagtcaatga tcaaggggga gctttccaag ttgtaaccac tatgactttt
     3661 cacgatcggc tattagatat ttacggtaat attcaagaac cgttatttag agctagagat
     3721 gttgcggaaa tgattgatca cagcaaaaca aggaacggat attatgacgt tcaatctatg
     3781 ctaagaaagg tagatgatga cgaaaaagtt aaaggcgtcc ctttagaggg tgctacgaaa
     3841 aatttccgta gcggtcaaaa agtttggttt ttaactgagc atggacttta tgaagtgctt
     3901 atgcggtcaa gcaaaccaaa agccaaagag tttagaaaag cagtcaaaaa cattctcaaa
     3961 gaaatccgtt tgaatggcta ctacatgcaa ggagagcttg tccaaaacag tcaaccgacc
     4021 ccagacattg atgatttaag ctatatcaag cagaagttaa ctgacttaca ggacgttgac
     4081 agcttacaag atttacggtg gaagatggct aagttatatc acgtcatcga aacactggat
     4141 gaatattaag aaataagaca acaaaaaaaa gcgcaataat acgcacggat aaaggagaaa
     4201 tgattatgaa agcaacaact tacaaagagt tgaagaaatg gattgatgaa ggtgttgatt
     4261 tagctgagct agcacagggt tacgctgaca aagtacctag tgtagaccgt gaacagtttg
     4321 aagcagtcac acagggaatt ttcaacgtat tggaaggcgt atcgctcatg cttgatgaca
     4381 aagtgctaat ctataatcgc aaagcagagc aaaagcgttt gaatgacatt gaacaaggtg
     4441 attattaatt aataatcaag agtaacaaaa aaagccttga actttggcga ggaacaggct
     4501 tttcacttca attaactaaa accataaaaa agcaagtatg ggatttagtt aggtatttat
     4561 ttaattatac cacaatcaac atcagggagc taccccttaa aactagcatg aatctagtat
     4621 aagaaacaag caactacaca gaaaataaga attaataagg aagataaaat gacatcagtt
     4681 agaaccgcac aattacttaa caatggttta ggattggttg gtcaagacct aaaagacgtc
     4741 ttatcagctc tagccaatag cgtcaatcca agtattgaaa aacagatttc tcacatcatg
     4801 atagatagag ctaatactgg cgaatggttg gcaaatgtag gcaaaaaatg aggtaagaca
     4861 taatgaaata tagagtagaa acaaatcctt tttcaaaaga tagatacact cctgaacagc
     4921 tagaaatgtt caaaaatcga caactcagca aaaataaagc agaagcctat ttcactcgac
     4981 tatataacca acatattgct tgggtgatta ttgctaacgt tatgacagaa tacgtcaata
     5041 aattcagaaa aagtgccacc agctttgaaa aagcatggga cgctttaggc tatcaacaaa
     5101 ccacagagat tgtctttaga gccgttaacg gtttaccttg ttcagagaaa gacacagggg
     5161 aactagaaac ttatttaagt gaggtatcgg catgatgcaa gaacttaacc tcacaccaac
     5221 acagacactt attttattca ttgttttagg gcttgttggg cttcttctta gcctttctaa
     5281 gccattaata gagattgact taccagaaga tacccaagta cctaaaccac ctcagaacgc
     5341 aaactatggg gcttatattc aatcacagaa ccattattac aattaggagg gaacagatgc
     5401 aattattatc acgagaagca gaattagagc tactagagaa ggtgggctgt taccttgaaa
     5461 aacgttggca acttgaacta gaaaacaaag atgattggga tttaattcca aggcaagatc
     5521 tactggaaaa actagacatt actggtacca cgttaaaaaa ttgggaaaaa gagggcttaa
     5581 agtcgtatca gtcgccattc gagaacagta agaaggtgta ttaccgtaaa agtgatattt
     5641 ataatttttt gagcgttgac taggaggact ttgtggatta tttagaggtc accatgacaa
     5701 aagattgcgt aaaaatattc aattttttgt ataaatatcc taccaaagca taccaaaagg
     5761 acgagtattt caaattactg tatatgcatc ccttggacag ctttttaaca agtttctccc
     5821 tatcgggcat ccgagtgaaa gttacagaca aacctgtttt agcagggtgg aaacttgtta
     5881 gggatataga ggttagaatt gctacggggg aactcttgga aatgattgaa gagctagaaa
     5941 tatgctattt aaggaagcac cagacggttt cttacgttga aattaagttc tatgtcgtcc
     6001 atctattgac ttatggtatt aggtcacgtt acgatatgca attttttacc aagttgttgt
     6061 tttgttgtgg ttatgatcaa gaaactgtta tcggtattta tagcaatata accaaaaata
     6121 ccagactttc aagggatttt ataactttac aggctaaact ttaccaaaca aagaaaggaa
     6181 cccatgagca ttaaagaaaa cattgacaaa ttagtatcta tggaatggac tgaatcaatt
     6241 tttgggcaat ctaaaatgaa agcagactat aaacgccaac tatctaaggt gattgagttg
     6301 gataccttaa gcaatataca ccataatggg gaaacctatg ctgtttcaaa tcaagaagtc
     6361 atgtacttaa gtgagagtaa aggtagaact cataagttta tttcaggaaa cgttaaaaca
     6421 aaatctaaag gaaagggaag agatagccaa aagtatctcg tggtttcctt tgaccatcaa
     6481 ttcccacctt ttgctaagtt agtggttgat tatctacttg gacgatttac tttttttaat
     6541 aacaaattat atgatgttaa caatcgtcaa gcgcaagtgt tagatgattt cactattcaa
     6601 tcgttatacg gcttcaaaaa ggataacccg ttcattttag agattctgga aggaatccac
     6661 caaaccttga atattagacc tgcgaaacaa cttgaagctt ataagatttc tgggagagat
     6721 ttcattattg atcttaagga acatcggtta taccgaacaa atcccagttc cgatagttct
     6781 ttctttaagt tttatgatgt cgattatcac acagcgatcg gtagtaagaa aaaagttggc
     6841 aagtttttag attatgttat cgcagaccat gatagcttgc acaatgcaac gttacaagcc
     6901 tattatatcg cacaggtcgc ttgtggactg aaatcgaaac aaaatttctt tatctctaaa
     6961 tctggtgtga gaactggtaa aggcttaaga catattgggc ttagcggtat ctttaataaa
     7021 atcgatgttg agttagactt attgattaaa ggtggctttg aagctagcaa tgcttggggt
     7081 gcttttgctg gtggagaaat ggctctagca acagaacaag gggatattgt aggcgataaa
     7141 atggagcgtg tgcttaaaat tattgctact gagaagaccc atatggctag aagtatcggt
     7201 ggcaatttag gaacagttaa tttaacaagt gttctgtgta ttgacacaaa ccgcactgtt
     7261 gcgctgtcgg atgaaatgaa tggtagggcg gttttgatcc aataccagaa tagaccagaa
     7321 ggggaaactg atttagaacg tgaaaaggcc tttgaagaat attggcaaac cttcactgaa
     7381 atggataaaa gccctaaaat tgaaggagtg ataggttttt tacttgtatc acttgattac
     7441 ttccgaaaaa atgacatgaa atttgattgg agagcggtcg aagtggtgaa cgatgatgat
     7501 ttagatgatt ttcaagttta cctgattaac catctttctg aaaaagactt tgtagagcgt
     7561 gactcttatg ttagagagct gtataactct acttacggga aaaataacaa ccaagccggc
     7621 caaagaatgc ggacgatagg ggttaagagt tatcgcaagc aaacaactgg aggacgcaaa
     7681 atacccggct ataaagtcga taatataaaa cgatttaaca aattctcagg aggagaaaaa
     7741 gaaaatacta atatctttga tatgtttgaa aagctaaacc aaccactacc gccatgaccc
     7801 agacagtagg ggttgtgggg ggtataaacc cagtggtatc aagggataga gtacaatcac
     7861 ccataa
//
LOCUS       DATXQK010000057         7627 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.057, whole genome shotgun sequence.
ACCESSION   DATXQK010000057 DATXQK010000000
VERSION     DATXQK010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7627
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.057"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            24..1742
                     /locus_tag="VC394_001506"
     CDS             24..1742
                     /locus_tag="VC394_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEP5994962.1"
                     /translation="MTYTENFQKWLDFEQLPDYLRQELLSMDEKTKEDAFYTNLEFGT
                     AGMRGYIGAGTNRINIYVVRQATEGLAKLIETKGEEAKKHGVAIAYDSRHFSPEFAFE
                     SAQVLAQHGIKSYVFEALRPTPELSFAVRHLNAYAGIMVTASHNPAPFNGYKVYGQDG
                     GQLPPADADALTDFIRAIENPFAVELADLDESKSSGLIQVIGEDVDMEYLREVKDVNI
                     NQDLINNFGKDMKIVYTPLHGTGEMLTRRALAQAGFESVVVVESQAKADPDFSTVKSP
                     NPESQAAFALAEELGREVDADVLVATDPDADRLGVEIRQPDGSYKNLSGNQIGAIIAK
                     YILEAHKTAGTLPENAALAKSIVSTELVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMFGFEESFGYLIKPFVRDKDAIQAVLLVAEIAAYYRSRGLTLADGIDEIYKE
                     YGYFAEKTISVTLSGVDGAAEIKKIMDKFRENGPKQFNNTDIVLLEDFQKQTATKNDG
                     TISNLTTPPSNVLKYTLADDSWIAVRPSGTEPKIKFYIATIGDTLDIAQEKIANIETE
                     INTFVG"
     gene            complement(1960..2916)
                     /locus_tag="VC394_001507"
     CDS             complement(1960..2916)
                     /locus_tag="VC394_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP5994963.1"
                     /translation="MSLVTIFALLVSSMLIYATPLIFTSIGGTFSERSGVVNVGLEGI
                     MVMGAFSGIVFNLEFAETFGKATPWIAVLVGGIVGLIFSLIHAVATINFRADHIVSGT
                     VLNLLAPSFAVFLVKAMYGKGQTDNIQQSFGKFDFPGLSQIPVIGDIFFKNTSLIGYF
                     AIAFSFFAWFLLYKTRFGLRLRSVGEHPQAADTLGINVYLMKYYGVMISGFLGGIGGA
                     VYAQSISVNFAVTTILGPGFIALAAMIFGKWNPVGAMLSSLFFGLSQSLAVIGAQLPL
                     LEKIPTVYLQIAPYMVTIIILAAFFGQAVAPKADGINYIKSK"
     gene            complement(2918..3982)
                     /locus_tag="VC394_001508"
     CDS             complement(2918..3982)
                     /locus_tag="VC394_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP5994964.1"
                     /translation="MSKNAQKIAVPLISVLLGFLLGAIIMVIFGYDPIWGYEGLFQIA
                     FGSVKNIGEIFRSMGPLILIALGFTVASRAGFFNVGLSGQALAGWISAGWFALLNPDM
                     PRPLLILMTALIGMIAGGIAGAIPGILRAYLGTSEVIVTIMMNYIILYVGNAIVQRGY
                     PESVKQSIDSTIQVSDNASYQTHWLSALTNNSRINIGIFFAIIAIALIWFLLNKTTLG
                     FEIRSVGLNPHASEYAGMSSKRTIILSMIISGALAGLGGVVEGLGTFENVFVQGSSLA
                     VGFDGMAVSLLAANSPLGIFFSSFLFGVLNIGAPGMNIAGIPPELVKVVTASIIFFVG
                     SHYLIERYIIRPKKLVKGGK"
     gene            complement(3975..5507)
                     /locus_tag="VC394_001509"
     CDS             complement(3975..5507)
                     /locus_tag="VC394_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5994965.1"
                     /translation="MTQHVIEMREITKKFGDFVANDHINLNVRKGEIHALLGENGAGK
                     STLMNMLAGLLEPTSGEIVINDKPVQIDSPSKSAKLGIGMVHQHFMLVEAFTVAENII
                     LGNEVVKNGCLDLNQASKDIKVLSEKYGLAINPSAKVSDISVGAQQRVEILKTLYRGA
                     DILIFDEPTAVLTPAEIKELMTIMKNLVKEGKSIILITHKLDEIRAVAERVTVIRRGK
                     SIETVDVAGATSQDLAEMMVGRSVSFTTSKKAAEPKDVVLSIKNLEVDENRGIPAVKG
                     LSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVKSGSIMIKNKEVTHLSSRKITELS
                     VGHVPEDRHRDGLILDLSLAENTALQTYYKQPLSQNGILNYTKINDYARQLMKEFDVR
                     GANELVPARGFSGGNQQKAIIAREVDRDPDLLIVSQPTRGLDVGAIEYIHKRLIKERD
                     KGKAVLVVSFELDEILNLSDRIAVVHDGKIQGIVLPENTNKQELGILMAGGSIHKEEG
                     HV"
     gene            complement(5646..6698)
                     /locus_tag="VC394_001510"
     CDS             complement(5646..6698)
                     /locus_tag="VC394_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEP5994966.1"
                     /translation="MNKKFIGLGLASVAVLSLAACGNRGASKGGASGKTDLKVAMVTD
                     TGGVDDKSFNQSAWEGLQSWGKEMGLQKGTGFDYFQSTSESEYATNLDTAVSGGYQLI
                     YGIGFALKDAIAKAAGDNEGVKFVIIDDIIEGKDNVASVTFADHEAAYLAGIAAAKTT
                     KTKTVGFVGGMEGTVITRFEKGFEAGVKSVDDTIQVKVDYAGSFGDAAKGKTIAAAQY
                     AAGADVIYQAAGGTGAGVFNEAKAINEKRSEADKVWVIGVDRDQKDEGKYTSKDGKEA
                     NFVLASSIKEVGKAVQLINKQVADKKFPGGKTTVYGLKDGGVEIATTNVSKEAVKAIK
                     EAKAKIKSGDIKVPEK"
     gene            complement(6792..7181)
                     /locus_tag="VC394_001511"
     CDS             complement(6792..7181)
                     /locus_tag="VC394_001511"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEP5994967.1"
                     /translation="MGTTDLVSCAVQASEYAYVPYSHFPVGAALKTKDGTIYTGCNIE
                     NVSFGLTNCGERTAIFKAISDGHKELVEIAIYGETMQPVSPCGACRQVMAEFFAPSSL
                     VTLIAKNGQTVEMTVGDLLPYSFTDLI"
BASE COUNT         2495 a         1589 c         1275 g         2268 t
ORIGIN      
        1 ataaatattg aaggagtatt tcaatgactt acacagaaaa ttttcaaaaa tggttagatt
       61 ttgagcagct tcctgactat cttcgtcaag aattactatc aatggatgaa aaaacaaaag
      121 aagatgcttt ttacacaaac ttagaatttg gtactgctgg tatgcgtggt tatattggtg
      181 ctggtacaaa tcgtattaat atttatgttg tccgtcaagc aaccgaagga ttagcaaaat
      241 taatcgaaac taagggagag gaagctaaaa aacatggtgt agccatcgct tatgattcgc
      301 gccacttctc tccagaattt gcttttgagt ctgcacaggt cttggcacag catgggatta
      361 aatcttacgt atttgaagct cttcgtccaa caccagaact ctcttttgct gttcgccatt
      421 taaatgccta tgctggtatt atggttactg caagccacaa tccagcacct ttcaatggtt
      481 ataaagtcta tggacaagac ggaggtcaac taccaccagc tgatgctgat gcactaacag
      541 actttatccg tgctattgaa aatccatttg cagttgaact tgctgattta gatgaaagca
      601 aatcatcagg tcttattcaa gttatcggtg aagatgttga tatggaatat cttagagagg
      661 ttaaagatgt taatatcaat caagatttaa tcaataactt tggaaaagac atgaaaattg
      721 tctatactcc tctccacgga actggtgaga tgttgacacg ccgtgccctt gctcaagccg
      781 gttttgaatc tgttgttgta gtcgaatcac aagctaaggc agatccagac ttttctactg
      841 ttaaatcgcc taaccctgaa agccaagctg cctttgctct tgctgaagaa ctcggacgag
      901 aagttgatgc agatgtctta gtagctactg atcctgatgc agaccgccta ggcgttgaaa
      961 ttcgccaacc agatggttct tataaaaacc tttctggaaa tcaaattgga gcgattattg
     1021 cgaaatacat cctggaagct cataaaacag caggaacact tccagaaaat gctgctctcg
     1081 ctaaatcaat cgtatcaaca gaattagtaa caaaaattgc cgaaagttat ggcgctacaa
     1141 tgtttaacgt tttaactgga ttcaaattca tcgctgagaa aattcaagag tttgaagaaa
     1201 aacataacca cacctatatg tttggttttg aagaaagctt cggatatctt attaaaccat
     1261 ttgtccgtga taaagatgct attcaagcag ttcttttggt tgctgagatc gcagcttatt
     1321 accgttcgcg tggtttaaca cttgcagatg gtattgatga aatttacaaa gaatatggct
     1381 attttgctga aaaaacgatt tcagtaacac tttcaggtgt ggatggagct gctgaaatta
     1441 agaagattat ggacaaattc cgtgaaaatg gtccaaaaca atttaacaat actgatattg
     1501 tattattaga agatttccaa aaacaaaccg ctactaaaaa tgatggaaca atatctaact
     1561 taacaactcc tccaagtaat gttttgaagt acacgttagc tgatgattca tggattgcag
     1621 ttcgcccttc aggaactgaa ccaaaaatca agttctatat tgccacaata ggtgatactc
     1681 ttgacatcgc tcaagaaaaa attgctaaca ttgaaacaga aatcaatacc tttgttggct
     1741 aaaacaactt aagagctatc agtaaaaacg aaagacaagc aaaaagaact aagccgctga
     1801 cttagttctt tttagtctat tcttgcttct attgtgatgg tgactggcaa tccaaacgct
     1861 tgcggtaagt cccaaaaaag cacccttaaa gctagaccta actatcttgc tttaggagtg
     1921 ctgttaaacc tcactaagag gcttactact aatataacgt tatttagatt taatatagtt
     1981 gattccatca gcttttggtg caactgcttg accaaagaaa gcagctaaaa taataatcgt
     2041 taccatataa ggtgcaatct gtaagtaaac cgttggaatc ttttctagta atggtaattg
     2101 agcaccaata acggctaaac tttgcgataa gccaaagaaa agactagata gcatggcgcc
     2161 aactggattc catttaccaa agatcatagc tgccaaagca ataaatccag gacctaagat
     2221 agttgttaca gcaaagttaa cggagattga ctgagcataa acagctccac ctattccacc
     2281 aagaaaacct gagatcatca caccatagta tttcatcaaa tagacgttaa taccaagtgt
     2341 gtcagctgcc tgagggtgtt ccccaactga tcgtaaacgc agaccaaacc tggttttata
     2401 aagcaaaaac caagcaaaga acgaaaaagc aatggcgaag taaccaataa ggctagtatt
     2461 tttaaagaaa atatcaccaa tcacaggaat ttgtgataaa cctgggaaat caaacttccc
     2521 aaaagattgt tgaatgttgt ctgtttgtcc cttaccgtac atagctttaa ccaagaagac
     2581 agcaaaagaa ggtgctaaca agttcaacac tgtaccactg acaatgtggt cagctcgaaa
     2641 gttgatggtt gctacagcgt gaatcaaaga aaaaatcaaa ccaacaatac caccgactaa
     2701 aacggctatc caaggcgttg cttttccaaa tgtttcagca aactctaagt tgaaaacaat
     2761 cccagagaac gctcccatga ccatgatacc ttctaaacca acattaacaa cgcctgagcg
     2821 ctctgagaaa gttcctccaa tacttgtgaa aatgagtggt gttgcataaa taagcataga
     2881 tgacaccaat aaggcaaaaa ttgttactaa actcatctta tttaccacct ttcactaatt
     2941 ttttagggcg aataatgtaa cgttcaatga ggtaatgtga accaacaaag aaaatgattg
     3001 atgcagttac tactttaaca agttctggtg gaatgcctgc aatattcatt cctggagcac
     3061 caatatttaa gacaccaaat aggaatgatg agaaaaatat tccaagcggt gaatttgctg
     3121 ctaataagct aactgccata ccgtcaaaac caactgctaa agaagatcct tgtacaaaaa
     3181 cattttcaaa ggttcctaga ccttcaacta ctcctccaag acctgcaaga gctccagaga
     3241 taatcattga taaaatgata gtacgttttg atgacatccc tgcgtattct gaagcatgtg
     3301 ggttgagacc aactgaacga atctcaaaac ctaaagtagt tttgttaagc aagaaccaga
     3361 ttagggcaat ggcaataatg gcaaagaaaa taccaatatt aatacgagag ttattagtta
     3421 gggctgataa ccaatgcgtt tgataactag cattatcact aacttgaata gttgaatcaa
     3481 tactttgttt gacactctca ggataaccac gttgaacaat agcatttccg acatataaaa
     3541 tgatgtagtt catcataatc gtaacgataa cttcacttgt accaagataa gcacgaagta
     3601 tccctggaat agctccagca ataccaccag caatcattcc gataagagct gtcataagaa
     3661 tcaataatgg acgaggcata tctggattta agagtgcaaa ccaacctgct gaaatccatc
     3721 cagctaaagc ctgtccagac aaaccaacgt taaagaagcc tgcacggcta gcaacagtaa
     3781 agccaagcgc tattaaaatc aatggcccca ttgaacggaa tatttctcca atatttttaa
     3841 cactaccaaa ggcaatttgg aataaacctt cataccccca gataggatca taaccaaaga
     3901 taaccataat aatagcgcca agtaaaaatc ctagcaagac agaaatcaat ggtacagcaa
     3961 ttttttgcgc atttttagac atgaccttcc tccttatgaa ttgaaccacc tgccattaaa
     4021 atacccaatt cttgtttatt ggtattttct ggcaatacga taccttgaat tttaccatca
     4081 tgaacaacag caattcgatc agataaattc aatatttcat caagttcaaa actaacaacg
     4141 aggacagctt tacctttatc acgttcttta atcaaacgct tatggatata ttcaatagct
     4201 cctacgtcaa gaccacgtgt tggttggcta acaataagta aatctggatc acggtccact
     4261 tcacgagcaa taatggcctt ttgttggtta ccaccagaaa atcctctagc tggtactagc
     4321 tcattggctc cgcgaacatc aaattctttc attaattgac gagcatagtc attaatttta
     4381 gtataattta agattccatt ttgacttaga ggttgtttgt aataggtttg taaggctgta
     4441 ttttcagcta aagaaagatc tagtatcaaa ccatctcgat gacgatcttc tggcacgtga
     4501 cccactgata actcagtaat tttgcgtgaa gacaaatgag tgacttcttt gtttttaatc
     4561 ataatacttc cagatttgac tttacgtaaa cctgtaatag cttgaattaa ctcagattga
     4621 ccatttccgt caattccagc aatccctaca atttcaccag ctcggacatc tagagatagc
     4681 cccttaactg ctggaatccc tctattttca tctacttcaa gatttttgat ggataagacc
     4741 acatcttttg gttcagcagc ttttttgcta gttgtaaagg aaaccgagcg tcccaccatc
     4801 atctcagcta aatcttgtga agttgctcct gccacatcaa ctgtttcgat actcttacca
     4861 cgacgaataa ctgtgacacg ctcggcgaca gcacgaatct cgtccagttt atgggtaatt
     4921 aaaataattg attttccttc tttcacgaga ttcttcatga tagtcatcaa ttctttaatt
     4981 tctgcaggtg ttaaaacggc agttggctca tcaaagataa gaatatctgc accacgatac
     5041 aaggttttca aaatctcaac acgttgttgt gctcctactg aaatatcaga aactttagct
     5101 gatggattga ttgcaaggcc atatttttcg gatagtacct taatatcttt cgatgcttgg
     5161 ttaagatcta agcacccatt ttttactact tcatttccta aaataatatt ctcagcaacc
     5221 gtgaaggctt ctactaacat gaaatgttga tgtaccattc cgataccaag cttagctgat
     5281 ttagatggcg aatcaatttg aactggcttg tcattaatga caatttctcc actagtaggc
     5341 tctaagagac cagctagcat attcatcaaa gttgatttac ctgccccatt ttcacccaat
     5401 aaggcatgaa tctcaccttt tctgacattt aagttaatgt ggtcatttgc aacaaaatca
     5461 ccaaattttt tcgtgatttc tctcatttca atgacatgct gtgtcatagg atcatttcct
     5521 ttcaatgcaa tcttttattt cagtaaagcc tatgatctat cacctatcac aaactttact
     5581 gagataaaaa ttatctcagt ttaaaaagag cgaccgacaa aggtcgcgct tcttgttctt
     5641 aaacactatt tttcaggaac tttaatgtca ccagatttaa tttttgcttt cgcttcttta
     5701 atagctttaa cagcttcttt tgaaacattt gtagttgcga tttcaacacc gccatctttt
     5761 agaccataga cagttgtttt tcctccaggg aattttttat ctgctacttg cttgttgatt
     5821 aactgaacag ctttaccaac ttctttgatt gatgatgcaa gtacaaagtt tgcttctttg
     5881 ccatctttag aagtgtattt tccttcgtct ttttgatcac ggtcaacacc aataacccaa
     5941 actttatcag cttcactacg tttttcatta atagcttttg cttcattaaa tacacctgct
     6001 ccagtgcctc ctgctgcctg gtaaataaca tcagcacctg ctgcatactg agctgctgcg
     6061 attgtttttc cttttgcagc gtcaccaaat gatccagcat aatcaacttt aacttggatt
     6121 gtatcgtcaa cagacttaac tcctgcttca aaaccttttt caaatcgagt tatgacagtt
     6181 ccttccatac cgcccacgaa accaactgtt tttgtttttg ttgtttttgc agctgcaatt
     6241 cctgcaagat aagcagcttc atggtcagca aaggtaacac tggctacatt atcttttcct
     6301 tcgataatat catcgataat aacaaactta actccttcat tatctccagc tgctttagca
     6361 atagcatctt tcaatgcaaa gccgatacca taaatcagtt gataccctcc tgaaactgct
     6421 gtatcgagat tagttgcata ctcagattca cttgtagatt gaaaataatc gaaacctgtt
     6481 cctttttgaa ggcccatttc tttaccccaa gattgcaggc cttcccatgc tgattgattg
     6541 aatgatttgt catctacacc accagtatcg gtaaccattg caacttttaa atcagttttt
     6601 cctgatgccc cacctttaga agcaccacga ttaccacaag cagctaaact cagcacagcc
     6661 actgacgcta aaccaagacc aataaatttc ttgttcatga atatgaaccc tcctaaaaaa
     6721 gatttctagc aaataagttg ctgaaagtaa atataaagcc aagacggcta gataacagaa
     6781 attaaagcag attatattaa atctgtaaaa gaatagggga gtaaatctcc aacagtcatt
     6841 tcaacagttt gaccattttt agcaattaaa gtgactaaag aagaaggagc aaaaaattct
     6901 gccattacct gacgacaagc cccacaagga gatacaggtt gcatagtttc tccataaata
     6961 gcaatttcaa ccaattcttt atgaccatca gatatggctt taaatatagc tgttcgctct
     7021 ccacaatttg tcagaccaaa actaacattt tcaatattac agccagtata gatcgttcca
     7081 tcttttgttt ttaaagctgc tcctactgga aaatgagagt aagggacata agcatactca
     7141 ctagcttgta cagcacaact tactaaatca gtagtcccca tttaacatca cctttcataa
     7201 tagctacatc tattgaaacc cctgtagata ctttaacgaa atatgcaccc gacttgatag
     7261 ataattaacg tgatctaact ttagcactta tatcatctcc gatagactgg taatttcttt
     7321 gctttgaagt aaacgaccgt tcaaaacgat atcaatctct tcagcactgt ttgaattaac
     7381 atctttaact tcaactagtt cactggcatc cgacgtttct ccaaaaagaa agactataac
     7441 ggtcccgtca gtacattaac gtcgacacct tagaagcata gactattcaa catggattga
     7501 tacagatatt tgtaaaatta taaatatctg tatcaatcac ttgatcaacc tgtgatcgaa
     7561 cgctacctat ctttagtagc gtatggttga ttgtcaccat aacaatgaca ccttagaaga
     7621 taatgat
//
LOCUS       DATXQK010000058         7427 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.058, whole genome shotgun sequence.
ACCESSION   DATXQK010000058 DATXQK010000000
VERSION     DATXQK010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7427
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.058"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            8..1144
                     /gene="dnaN"
                     /locus_tag="VC394_001512"
     CDS             8..1144
                     /gene="dnaN"
                     /locus_tag="VC394_001512"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEP5994968.1"
                     /translation="MIQFSINRTLFIHALNATKRAISTKNAIPILSSIKIEVTSTGVT
                     LTGSNGQISIENTIPVSNENAGLLITSPGAILLEASFFINIISSLPDISINVKEIEQH
                     QVVLTSGKSEITLKGKDVDQYPRLQEVSTENPLILKTKLLKSIIAETAFAASLQESRP
                     ILTGVHIVLSNHKDFKAVATDSHRMSQRLITLDNTSADFDVVIPSKSLREFSAVFTDD
                     IETVEVFFSPSQILFRSEHISFYTRLLEGNYPDTDRLLMTEFETEVVFNTQSLRHAME
                     RAFLISNATQNGTVKLEITQNHISAHVNSPEVGKVNEDLDIVSQSGSDLTISFNPTYL
                     IESLKAIKSETVKIHFLSPVRPFTLTPGDEEESFIQLITPVRTN"
     gene            1219..1416
                     /locus_tag="VC394_001513"
     CDS             1219..1416
                     /locus_tag="VC394_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEP5994969.1"
                     /translation="MYQIGSFVEMKKPHACVIKETGKKANQWKVLRVGADIKIQCTNC
                     QHVIMMSRYDFERKLKKVLQP"
     gene            1746..2861
                     /gene="ychF"
                     /locus_tag="VC394_001514"
     CDS             1746..2861
                     /gene="ychF"
                     /locus_tag="VC394_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEP5994970.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTELITPKKTVPTTFEFTDIAGIVKGASRGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPIADIDTINLELILADLESINKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTEDEAKVVKGLFLLTTKPVLYVANVDEDKV
                     ANPDGIDYVKQIRDFAATENAEVVVISARAEEEISELDDEDKEEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGIKAPQAAGIIHSDFERGFIRAVTMSYDD
                     LMTYGSEKAVKEAGRLREEGKEYVVQDGDIMEFRFNV"
     gene            2931..3500
                     /locus_tag="VC394_001515"
     CDS             2931..3500
                     /locus_tag="VC394_001515"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEP5994971.1"
                     /translation="MVKMIVGLGNPGSKYEKTKHNIGFMAIDNIVKNLDVTFTDDKNF
                     KAQIGSTFINHEKVYFVKPTTFMNNSGIAVKALLTYYNIDITDLIVIYDDLDMEVSKL
                     RLRSKGSAGGHNGIKSIIAHIGTQEFNRIKVGIGRPLKGMTVINHVMGQFNTEDNIAI
                     SLTLDRVVNAVKFYLQENDFEKTMQKFNG"
     gene            3503..7006
                     /gene="mfd"
                     /locus_tag="VC394_001516"
     CDS             3503..7006
                     /gene="mfd"
                     /locus_tag="VC394_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEP5994972.1"
                     /translation="MNILELFSQNKKVQSWHSGLTTLGRQLVMGLSGSSKALAIASAY
                     LDDQKKIVVVTSTQNEVEKLASDLSSLLDEELVFQFFADDVAAAEFIFASMDKALSRI
                     ETLQFLGNPKSQGVLIVSLSGLRTLLPNPDVFTKSQIQLTVGEDYDSDTLTKQLMTIG
                     YQKVSQVISPGEFSRRGDILDIYEITQELPYRLEFFGDDIDSIRQFHPETQKSFEQLE
                     GVFINPASDLIFEASDFQRGIEQLEKALQTAQDDKKSYLEDVLAVSKNGFKHKDIRKF
                     QSLFYEKEWSLLDYIPKGTPIFFDDFQKLVDKNARFDLEIANLLTEDLQQGKALSNLN
                     YFADNYRELRHYKPATFFSNFHKGLGNIKFDQMHQLTQYAMQEFFNQFPLLIDEIKRY
                     QKNQTTVIVQVESQYAYKRLEKSFQDYQFRLPLVSANQIVSRESQIVIGAISSGFYFA
                     DEKLALITEREIYHKKIKRRARRSNISNAERLKDYNELAVGDYVVHNVHGIGRFLGIE
                     TIQIQGIHRDYVTIQYQNSDRISLPIDQIGSLSKYVSADGKEPKINKLNDGRFQKTKQ
                     KVARQVEDIADDLLKLYAERSQQKGFSFSPDDDLQRAFDDDFAFVETEDQLRSIKEIK
                     ADMESMQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVAVLVPTTVLAQQHYENFKA
                     RFENYPVEVDVLSRFRSKKEQAETLERVRKGQIDIIIGTHRLLSKDVVFSDLGLIVID
                     EEQRFGVKHKETLKELKTKVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPVQ
                     TYVLENNPGLVREAIIREMDRGGQIFYVYNKVDTIEKKVSELQELVPEASIGFVHGQM
                     SEIQLENTLIDFINGDYDVLVATTIIETGVDISNVNTLFIENADHMGLSTLYQLRGRV
                     GRSNRIAYAYLMYRPDKVLTEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNILGA
                     SQSGFIDSVGFEMYSQLLEQAIASKQGKTTVRQKGNTEINLQIDAYLPDDYISDERQK
                     IDIYKRIREIQSREDYLNLQDELIDRFGEYPDQVAYLLEIALLKHYMDNAFAELVERK
                     NNQVIVRFEVTSLTYFLTQDYFEALSKTHLKAKISEHQGKIDIVFDVRHQKDYRILEE
                     LMLFGERLSEIKIRKNNSVFK"
     gene            7168..>7427
                     /locus_tag="VC394_001517"
     CDS             7168..>7427
                     /locus_tag="VC394_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEP5994973.1"
                     /translation="MRLDKYLKVSRLIKRRSVAKEVADKGRIKVNGILAKSSTNIKLN
                     DHIEISFGNKLLTVRVIEIKDSTKKEDALKMYEIISETRITL"
BASE COUNT         2552 a         1127 c         1426 g         2322 t
ORIGIN      
        1 agttctcatg attcaatttt caattaatcg cacattattt attcatgctt taaatgcaac
       61 taaacgtgct attagcacta aaaatgccat tcctattctt tcatcaataa aaattgaagt
      121 cacttctaca ggagtaactt taacagggtc taacggtcaa atatcaattg aaaacactat
      181 tcctgtaagt aatgaaaatg ctggtttgct aattacctct ccaggagcta ttttattaga
      241 agctagtttt tttattaata ttatttcaag tttgccagat attagtataa atgttaaaga
      301 aattgaacaa caccaagttg ttttaaccag tggtaaatca gagattacct taaaaggaaa
      361 agatgttgac cagtatcctc gtctacaaga agtatcaaca gaaaatcctt tgattttaaa
      421 aacaaaatta ttgaagtcta ttattgctga aacagctttt gcagccagtt tacaagaaag
      481 tcgtcctatt ttaacaggag ttcatattgt attaagcaat cataaagatt ttaaagcagt
      541 agcgactgac tctcatcgta tgagccaacg tttaatcact ttggacaata cttcagcaga
      601 ttttgatgtg gttattccaa gtaaatcttt gagagaattt tcagcagtat ttacagatga
      661 tattgagacc gttgaggtat ttttctcacc aagccaaatc ttgttcagaa gtgaacacat
      721 ttctttttat acacgcctct tagaaggaaa ttatcccgat acagatcgtt tattaatgac
      781 agaatttgag acggaggttg ttttcaatac ccaatccctt cgccacgcta tggaacgtgc
      841 cttcttgatt tctaatgcta ctcaaaatgg tactgttaag cttgagatta ctcaaaatca
      901 tatttcagct catgttaact cacctgaggt tggtaaggta aacgaggatt tagatattgt
      961 tagtcagtct ggtagtgatt taactatcag cttcaatcca acttacctta ttgagtcttt
     1021 aaaagctatt aaaagtgaaa cagtaaaaat tcatttctta tcaccagttc gaccattcac
     1081 cctaacacca ggcgatgagg aagaaagttt tatccaatta attacaccag tacgaacaaa
     1141 ctaagtaaga aaaagctccc ttttaggagt ttttttgtta ttataaatat taacgataat
     1201 ataagtggag aaaagggaat gtatcaaatt ggatcatttg ttgaaatgaa aaaacctcat
     1261 gcttgtgtaa ttaaagaaac tggtaaaaag gctaatcaat ggaaggtact gagagtagga
     1321 gctgatatta aaattcagtg tactaactgt cagcatgtga ttatgatgag tcgttatgat
     1381 tttgagcgaa aattaaaaaa agtactgcaa ccatgaaagt ccttttaaat aaataattct
     1441 agctatattt gcaaccaata ctttcctaaa aaattgctag tatgccgttg gaaaattagc
     1501 tattctaatg ttatcgaaag aagaaagatg gccattgaca atgacgaaag ttgtagcaca
     1561 gttacttagt ttctcggcaa actatatcaa aatgagaata atacaccaaa ctttgataat
     1621 ttaaatgaag gtgaaagagt ggcataatga taagcaaatc tgaaattttc ttcttattca
     1681 taccgctttt tcttctattt tttggtataa taatcttgat tgaaatttga atggagattc
     1741 gctaaatggc tttaacagca ggtattgtag gcttaccaaa tgttggtaaa tcaaccttat
     1801 ttaacgcaat tacaaaagca ggagcagaag ctgctaatta tccttttgcg actattgatc
     1861 ccaatgttgg tatggtagag gttccagatg agcgtctgca aaaattgaca gaattgatta
     1921 cacctaaaaa aacagttccg acaacctttg aatttactga tattgcaggt atcgttaaag
     1981 gtgcttctag aggggaaggt ctaggaaata aatttttagc taatattcgt gaagtcgatg
     2041 ctattgttca tgtggtacgt gcttttgatg atgaaaatgt catgcgtgaa caaggtcgtg
     2101 aggatgcttt cgttgatcct atagcagata ttgacactat taatcttgaa ttaatcttag
     2161 ctgatttaga gtcaatcaat aaacgttatg cgcgtgttga aaaaatggca cgaactcaaa
     2221 aagataaaga atcagtagca gagtttaatg ttttgcaaaa gattaaacct gttttggaag
     2281 atgggaaatc agctaggaca attgagttta cagaagatga agcaaaagtt gttaaaggtc
     2341 tctttttatt aacaactaaa cctgttttat atgtagctaa tgtcgatgaa gataaagttg
     2401 ctaatccaga tggtattgat tatgtcaaac aaattcgtga ctttgcagct actgaaaatg
     2461 ctgaagtagt tgttatctca gcgcgtgcag aagaagaaat ttcagagctt gacgatgagg
     2521 ataaagaaga atttttggaa gctatcggtc ttactgaatc aggcgttgat aaattaacca
     2581 gagcagctta tcatctctta ggccttggaa cctattttac agcaggtgaa aaagaggttc
     2641 gtgcttggac gtttaagcgt ggtataaaag ctccacaagc tgctggtatt atccattcag
     2701 attttgaaag aggttttatt cgtgcagtaa ccatgtctta tgatgatcta atgacctacg
     2761 gttcagaaaa agccgtcaaa gaagctggac gcttgcgtga agaaggaaaa gaatatgttg
     2821 tccaagatgg ggacatcatg gaattcagat ttaatgtgta attatattaa aacaatatca
     2881 gaaggttgga agaacattcc agcccttttg gcattttaga aagagaaaat atggtaaaaa
     2941 tgattgttgg tctgggaaat ccaggctcta aatatgaaaa aacaaagcac aatattggtt
     3001 ttatggctat tgacaatatt gtcaagaacc ttgacgttac ctttacagat gataaaaatt
     3061 ttaaagcaca gataggaagt acttttatta atcatgaaaa agtttacttt gtgaaaccta
     3121 ctacttttat gaataatagc ggcatagcag taaaagcatt actaacctac tataatattg
     3181 acataacaga tttaattgtt atctatgatg atttagacat ggaagtcagt aaattacgtt
     3241 tacgtagtaa gggttcagca ggaggacata atggcattaa gtcaatcatt gcccacattg
     3301 gaactcagga atttaaccga atcaaagttg gtattggacg acctttaaaa ggtatgactg
     3361 ttattaacca tgtgatgggc caattcaata ccgaagataa tattgctatt tcgttaactc
     3421 ttgacagagt tgtcaatgct gtcaagtttt atttacaaga aaatgatttt gaaaaaacaa
     3481 tgcagaaatt taatggataa tcatgaatat tttagaatta tttagtcaga ataagaaagt
     3541 ccaatcctgg cactctggat taaccacctt aggaagacaa ctggtaatgg ggttatcggg
     3601 ttcaagtaaa gcattggcta tagcttccgc ttatttagat gatcaaaaaa aaatagttgt
     3661 ggttacatca actcaaaatg aggttgaaaa attagccagc gatttatcta gtttacttga
     3721 tgaagaactt gttttccaat tttttgcaga cgatgtggct gcagcggaat ttatctttgc
     3781 gtcaatggat aaagctctat caagaataga aaccctgcaa tttttaggga atcctaaatc
     3841 tcagggcgtt ttaattgtta gtttatcagg cttaagaact ttattgccaa acccagatgt
     3901 ttttacaaag agtcagattc aactaacagt tggagaagat tatgatagtg atactcttac
     3961 taaacaactg atgacaattg gctatcagaa ggtctcacag gtcattagtc cgggagaatt
     4021 tagccgtcga ggggatattt tagatatcta tgagattaca caagaattgc cttatcgatt
     4081 ggaatttttt ggcgatgata ttgatagtat taggcaattt catccagaaa ctcaaaaatc
     4141 ttttgaacaa ctagaaggtg tttttattaa tccagcaagt gatcttattt ttgaggctag
     4201 tgattttcaa cgtggcattg agcaattaga aaaggctcta caaacagcac aagatgataa
     4261 aaaatcttat ttagaagatg tattagctgt ttcaaaaaac ggttttaaac ataaggatat
     4321 ccgtaaattt caatcattat tttacgaaaa agagtggtca ttattagatt atattcctaa
     4381 gggaacgcca atcttttttg atgattttca aaaactagtt gataaaaatg caagatttga
     4441 tttagagatt gctaatctct tgacagaaga tttacagcaa ggaaaggctc tttccaatct
     4501 taactacttt gcagataatt atcgagagct taggcactat aagccagcga ccttcttttc
     4561 aaattttcat aagggacttg gaaatatcaa atttgatcag atgcatcagc taactcagta
     4621 tgccatgcag gaatttttta atcaatttcc tttgttgatt gatgagatta aacggtatca
     4681 aaaaaatcaa acaacggtta ttgtacaggt agagtctcag tatgcttaca aacgacttga
     4741 aaaatctttt caagattacc aatttcgcct tcctttagtg agtgctaatc aaattgtttc
     4801 acgtgaatca caaattgtaa ttggagctat ctccagtggt ttttattttg ctgatgaaaa
     4861 gttagcgtta atcacagagc gtgaaattta tcataaaaag atcaaacgac gcgctagacg
     4921 atctaatatt agcaatgctg agcgtttgaa agattacaat gagttagcag taggtgatta
     4981 cgtggttcat aatgtccatg gtatcggtcg ctttcttgga attgaaacga ttcagattca
     5041 gggaatccat cgagattatg ttactattca atatcaaaat tcagaccgta tttctcttcc
     5101 aattgaccaa attggtagct tatcaaagta cgtttctgct gatggaaaag aacctaaaat
     5161 taataaactc aatgatggtc gtttccaaaa gacaaagcaa aaggtcgcta gacaagtaga
     5221 ggatattgct gatgaccttc taaaattata tgctgaaaga agtcagcaaa aaggattttc
     5281 attttcacca gatgatgact tgcagcgcgc ttttgatgat gattttgctt ttgtagaaac
     5341 agaagatcaa cttaggtcta taaaggaaat taaagctgat atggagagca tgcagcctat
     5401 ggatcgtctt ttagtaggcg atgtaggatt tggtaagaca gaagtagcta tgagggcagc
     5461 ctttaaagcg gtgaacgatc acaaacaagt agctgtctta gttccaacca cagtcttggc
     5521 ccagcagcat tatgaaaact tcaaagcacg ctttgaaaat taccctgttg aggttgacgt
     5581 cttaagtcgt ttccgtagta aaaaagagca agctgaaaca ctagaacgtg tacgaaaagg
     5641 tcaaattgac attattatcg gaacccatcg actcctatca aaagatgtgg tcttttctga
     5701 tttaggattg attgtgattg atgaggaaca acggtttggt gttaagcata aagaaacttt
     5761 aaaggaatta aaaactaagg ttgacgtctt aaccttaaca gctactccga ttcctagaac
     5821 cttacacatg tctatgttag gtatccgaga tttatcggtt attgagaccc caccaaccaa
     5881 tcgttatcct gttcaaacct atgttttgga aaataatcca ggtctcgtta gagaagctat
     5941 cattcgtgaa atggatcgtg gaggacaaat tttttacgtt tacaataaag ttgacactat
     6001 tgaaaagaaa gtttcagagc tacaagaatt agtcccagaa gcttctatag gttttgttca
     6061 tgggcaaatg agtgaaattc aacttgaaaa taccttgatt gactttataa atggtgatta
     6121 tgatgtcctt gtggctacaa caatcatcga aacaggagtt gacatttcta atgtaaacac
     6181 tttgtttatt gagaatgctg atcatatggg attgtcaact ttatatcaac tacgagggcg
     6241 cgtcggaaga agtaatcgta ttgcttatgc ttacctgatg tatcgtcctg ataaggtcct
     6301 aacagaggtt tctgaaaagc gtttagaggc tattaaaggc tttactgaat taggttcagg
     6361 cttcaagatt gctatgcgag atttgtctat acgaggagca ggtaatattt taggggcttc
     6421 tcagagtggc tttattgatt cggtcggttt tgaaatgtat tctcagttat tggaacaggc
     6481 tattgctagc aagcaaggaa aaacgactgt tcgccaaaaa ggtaatactg aaatcaatct
     6541 tcagattgac gcctatttgc cagatgatta catatcagat gagcgtcaaa aaattgacat
     6601 ttacaagcgt attcgagaaa ttcaatcaag agaagattat ctcaatttgc aggatgagct
     6661 gatagatcgt tttggagagt atcctgatca agttgcctat ttgttagaga tagctctgct
     6721 aaaacattat atggacaatg cctttgcgga attagttgaa cgcaaaaata atcaggtcat
     6781 tgttcgattt gaagtaactt ctttaactta tttcttgact caggattatt tcgaagcctt
     6841 atcgaaaacc catcttaaag ctaaaattag tgaacatcag gggaaaatcg atatcgtctt
     6901 tgacgttcgc catcaaaaag attatagaat tttagaagaa ttgatgttat ttggagaaag
     6961 gcttagtgag ataaaaatca gaaaaaacaa ttcagttttt aaataataat ttgaaacaac
     7021 ttttagactg aatagtatta atgacaatgg aacgaactag ctctttatac ttttgtcaaa
     7081 aatctaagat ctttgctgcg aaattaggat cttttctacc atttaaaaaa taaaaatgat
     7141 aaaatgaaga ggattgtagg agaaattatg agactagata aatatctaaa ggtatcgcgc
     7201 cttattaaac gtcgttcagt agcaaaagaa gttgcggata agggacgaat taaagttaat
     7261 gggatacttg ctaaaagttc aacgaatata aaactaaatg atcacattga aattagtttt
     7321 ggaaataaat tactgacagt aagagtcatt gaaataaaag atagtacaaa aaaagaagat
     7381 gctcttaaga tgtatgagat aatcagtgaa acaaggataa cattaaa
//
LOCUS       DATXQK010000059         7339 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.059, whole genome shotgun sequence.
ACCESSION   DATXQK010000059 DATXQK010000000
VERSION     DATXQK010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7339
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.059"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(54..563)
                     /locus_tag="VC394_001518"
     CDS             complement(54..563)
                     /locus_tag="VC394_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008088030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994974.1"
                     /translation="MELFNTIIGVIALIVAVIALVHSIYYNMVKIKLSDCYISRVDKG
                     YDWMYDFSISNLSNVSVIIKKIELYNKDGKLISDNGFNPFQKYEADMQNEADDYYGLS
                     MPNYYMPLDYQWESSPFKSDTEVYPSSRENFSYYLDEKPVKIKITTDKRIHQFRKYQL
                     FFPHFDNNS"
     gene            complement(822..1031)
                     /locus_tag="VC394_001519"
     CDS             complement(822..1031)
                     /locus_tag="VC394_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994975.1"
                     /translation="MNLEELTPLLNSIDDFETVILHSLVGDFVIDHWIEPNRKNETLI
                     FMHNDQTTELKLSAILGTSTIPKSL"
     gene            complement(1181..1420)
                     /locus_tag="VC394_001520"
     CDS             complement(1181..1420)
                     /locus_tag="VC394_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994976.1"
                     /translation="MDTKEFMKVVSKHINQNFNVDNQLVEFVVAELNQMNAPITQKQA
                     QHIVNILEYVSKSTSKSTIAAMTNALLELGVLKGD"
     gene            1587..1772
                     /locus_tag="VC394_001521"
     CDS             1587..1772
                     /locus_tag="VC394_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP5994977.1"
                     /translation="MWGKIEALLIEKKMTKYELSQKAGLNQNCLIDLKKGRKKSLKFD
                     DVVKIADVLGVSLDEFR"
     gene            1851..2147
                     /locus_tag="VC394_001522"
     CDS             1851..2147
                     /locus_tag="VC394_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994978.1"
                     /translation="MAIEIFGPEFRKKLLEDLIALNMEAIKIAQTKNAKSIEWITMKR
                     LEKETGWGRTKLTQWREQGKFNFKRSSENGKVLYDLADVNRFLRTSGYEKGETT"
     gene            2144..2281
                     /locus_tag="VC394_001523"
     CDS             2144..2281
                     /locus_tag="VC394_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994979.1"
                     /translation="MKLLDFIFAKPKKQEPIKHDTLRASSEKQWAEFDAYMRKQYGRQ
                     A"
     gene            2362..2691
                     /locus_tag="VC394_001524"
     CDS             2362..2691
                     /locus_tag="VC394_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994980.1"
                     /translation="MKTKIEIMRDFFKENPESTQRQASEKLGFTENTVKQYIWRDAKR
                     GYCIKDEEGRVTYLNIEDELSLINEWKSEIRRELIEQLLSANRHETSSEQIRMNAKTI
                     NQILGEI"
     gene            2691..2885
                     /locus_tag="VC394_001525"
     CDS             2691..2885
                     /locus_tag="VC394_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994981.1"
                     /translation="MEGMKKYINGQIKLIDTIEECVKEKWPEFDDKKVHKISLAIYQG
                     WSLTDINSTLEAIEMDLDRK"
     gene            2882..3166
                     /locus_tag="VC394_001526"
     CDS             2882..3166
                     /locus_tag="VC394_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5994982.1"
                     /translation="MRIYVNKKGKPSVEFEFEDRRGGMFDTRLMLKESPLKEEFKADM
                     YKAIDDVLKKYEDIFDTPIFKELIIGKEEDIRKMIDYDDKFTELFGGIRH"
     gene            3163..3846
                     /locus_tag="VC394_001527"
     CDS             3163..3846
                     /locus_tag="VC394_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEP5994983.1"
                     /translation="MKITKATEITKTHNWRILIYGKPGNGKTYLTNYLKGKTLILDMD
                     HSSKTIAGNENIDIIQFDRTHPSDFMTEFLTELPELIKEYDNLVIDNITSWQSDWFIE
                     QGRKSKNGITNELQQYNMWTNYYLRVLTTIYSQPTNIFVTAWESTQDLTLESGQIITQ
                     YVPDIRKQVLSQTLGLTDVVGRIQVNPKTGGHGILLQGSDGLYAKNRLDNRTVCKAEE
                     LFNFEGSDA"
     gene            3882..5285
                     /locus_tag="VC394_001528"
     CDS             3882..5285
                     /locus_tag="VC394_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017770133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEP5994984.1"
                     /translation="MNEARRHILKHNVMIVSPPGSGKSVVISDIAKSATQKNGHVLFL
                     VHRKELIDQITNSFKFHGIDMNKVDLMTVGKAKNRLDKLTKPTLIITDEGHHGKASTY
                     QIIYEYFSDVPRIGFTATPWRLSGDGFTDTYDVMVLGKTVEWLINNNKLAPYDYYSVL
                     SIDTAKLKVQNGDYSNKSIDESFGKKIFGDVVQEYIKKANGQKAILYAHSVEASQAFA
                     KEFQSMGINAIHADAKTPKAKRDKIMKDFRDGKIQVICNVDLISEGFDVPDCTVTILC
                     RPTKSLVLFLQQSMRSMRYQPNKKAIILDHVGNWNIHGLPDTPHHWENYFRGGWKKKS
                     NKTNTVHAKECSVCSALWPLSQQLCELCNHDFGLKEKQEKERIEAELELIKRERFRIK
                     QLANKKFGKDLKTNWEIAQARVKDAGKGKPLYKLIYFYLKTDWVETNVNELAEVTGKS
                     EKEIYSAYNWLKKKLRG"
     gene            5290..5772
                     /locus_tag="VC394_001529"
     CDS             5290..5772
                     /locus_tag="VC394_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000896928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HEP5994985.1"
                     /translation="MAGFTTDFSEVKEHAEFKEQPYEMIVYDAYEAVNERNGKKRVVI
                     DYVVRNDIKQEMQNYHLWDEQYPNSQTGKYHIGILMGKAKALGIKEGQHYDSFEAFLN
                     DFKGRTAKVTVKLDEYNGNKYPKVRYANQSDVPNSYHVWKEKATGFTQAEIEEDDLPF
                     "
     gene            5790..7339
                     /locus_tag="VC394_001530"
                     /pseudo
     CDS             5790..7339
                     /locus_tag="VC394_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017876.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage resistance protein"
BASE COUNT         2744 a         1148 c         1400 g         2047 t
ORIGIN      
        1 aaatgctcct aacggcagca cttatcttaa ttttattaac gaatatcgct atcttaacta
       61 ttattgtcaa aatggggaaa gaataactga tattttcgga attgatgaat acgcttatca
      121 gttgtgattt tgattttaac tggcttttca tctaaatagt aagagaaatt ttctcgacta
      181 gatggatata cttcagtatc tgacttaaat ggcgatgact cccattggta atctaatggc
      241 atataataat tcggcattga caatccataa taatcatcag cttcattctg catgtcagct
      301 tcgtattttt gaaagggatt gaagccatta tcgcttatta gttttccatc tttgttgtaa
      361 agttcaattt ttttaataat gactgaaaca ttcgataagt tactgatact aaaatcatac
      421 atccaatcgt aacctttatc tactcttgaa atgtaacaat cagataattt tatcttaacc
      481 atgttgtagt agatagagtg aacaagagca atcacagcaa caattagcgc aatgacgccg
      541 ataattgtat taaataattc catttttatt cttccaatcg tttttatttt aattatacca
      601 aaatagaaag gacacatatg aacgaaatag aaaacaaact agaagaactt gaagaaatgg
      661 taataaacat ggatgaagta gatgtagtga ttccatggaa gatagcaaaa aacctgctac
      721 aaagagcagg ttacttatca gaagaggaac atcgcctact aagttggaga ctcggaaaat
      781 caaaatatgc aggcaaacgt tccgaaaaag tccgcaattt gctagaggga cttaggaata
      841 gtggaagttc cgagaatagc tgataatttt aattctgttg tttggtcatt gtgcatgaaa
      901 atgagagttt catttttgcg attaggctca atccaatgat caataacaaa gtctccgaca
      961 agactatgta aaataacagt ttcaaaatca tcaatactat tcaatagagg agttaattct
     1021 tctaaattca tttagttacc ctccttccta ataggataac tacattatac aacagaaagg
     1081 aagcacatga gaccaaaacg atatccgtat agcggagaga aaaagagagc ctatcataag
     1141 atagaccctg aactgataaa aaccggtaaa gttgattgca tcaatctccc ttaagtacgc
     1201 caagctctaa caatgcattt gtcatagctg cgatagtaga tttagaggtt gacttagaga
     1261 catactccaa aatattaacg atatgctgag cttgcttttg tgtaataggt gcattcattt
     1321 gattaagttc cgcaacgaca aattcaacta attggttgtc aacattgaaa ttttggttga
     1381 tatgttttga gacaactttc ataaattctt ttgtatccat aattaatctc ctttcttgct
     1441 tatttgacta aacaagatta gaagagtaga ggtctctcga gagatcttgt tgaatcatac
     1501 gaatattata tcaagactat attgaaaaat caatatattg tatataaaaa gttatcaaca
     1561 actacatgtt gtgcttagga ggaattatgt ggggtaaaat cgaagctcta ttaattgaga
     1621 aaaaaatgac caaatatgaa ctgtcacaaa aggctggatt gaaccaaaat tgcttgattg
     1681 atctaaagaa aggaagaaaa aaatcattga aatttgatga tgtcgttaaa attgccgatg
     1741 tcttaggtgt cagcttagac gaatttagat aacaaaaaaa gtccgacggc aatcggactc
     1801 aaaacaaatt ttaatttact taattataac ataagagaga gggaaaaact atggccattg
     1861 aaatatttgg acctgagttt agaaaaaaac tgcttgaaga tttaatcgct ctaaatatgg
     1921 aagcgataaa aatagcgcag accaaaaacg ccaagtctat tgaatggata accatgaagc
     1981 ggctagaaaa agaaactgga tgggggcgaa ctaaattgac ccagtggaga gaacaaggga
     2041 aatttaactt taaaaggtca tcagaaaacg ggaaagtact atatgaccta gcagatgtta
     2101 atagattttt acggaccagt ggatatgaaa aaggagaaac aacatgaaat tattagattt
     2161 tatttttgca aaaccaaaaa aacaggaacc gattaagcac gacacgctta gagcatcatc
     2221 tgaaaagcag tgggccgaat ttgatgccta tatgaggaaa cagtatggac gacaagctta
     2281 atcagatatt gatagccatg cgaaactacc gcacaaatgg tgatgatagc aagtatttag
     2341 aggatatgga ggtcatttta aatgaaaaca aaaattgaaa tcatgcgtga tttttttaaa
     2401 gaaaatcctg aatcaactca acggcaagcc tcagaaaagc ttggatttac ggaaaataca
     2461 gttaaacaat atatttggag agatgctaag cgtggctact gtattaaaga cgaagaaggt
     2521 cgtgtaactt atctaaacat tgaagatgaa ttatcgctta ttaatgaatg gaaaagtgaa
     2581 attagaagag aattgattga acagttacta tcagctaatc gacatgaaac gtcaagcgaa
     2641 caaattcgta tgaacgctaa aacaatcaat cagattttag gagaaattta atggaaggta
     2701 tgaaaaagta tatcaatggt cagattaaac taattgatac tattgaagaa tgtgtcaaag
     2761 aaaaatggcc tgaatttgat gataaaaaag tacataagat atctttagct atttaccaag
     2821 gatggtccct tacagatatt aatagcacac tcgaagcgat agaaatggac ttggatagaa
     2881 aatgagaatt tatgtaaaca aaaaaggtaa accatctgtt gaatttgagt ttgaagatcg
     2941 acgcggtggc atgtttgata caagattaat gttaaaagaa tcaccactca aagaagaatt
     3001 taaagccgat atgtacaagg caattgatga tgttttaaaa aaatacgaag acatttttga
     3061 tactcctatt tttaaagaat taataattgg gaaagaagaa gatattagaa aaatgattga
     3121 ttacgatgat aaatttaccg aactttttgg agggattaga cattgaagat tactaaagcg
     3181 acagaaatta caaaaaccca taattggcgc atattaatct atggtaaacc cggaaacggg
     3241 aaaacttact taactaatta tttaaaaggc aagacactga ttttagacat ggaccattcg
     3301 tctaaaacga ttgccggaaa cgaaaacatt gatattatcc aatttgacag gacgcaccct
     3361 agcgacttta tgaccgaatt cttgacagaa ctaccagaac ttatcaaaga atatgacaat
     3421 ttagtcattg ataatataac aagttggcaa tcagactggt ttatcgaaca aggtcgtaaa
     3481 tccaaaaatg gaatcacaaa cgaattgcaa caatacaaca tgtggaccaa ttactattta
     3541 cgagtattga ctaccattta cagccaacct actaatattt ttgtgactgc ttgggaatca
     3601 acgcaagatt taacgctcga gagcggacaa attatcacgc aatacgtgcc agatatccgc
     3661 aaacaagtcc taagccaaac gctaggttta accgatgttg tcggacgtat tcaagtaaat
     3721 ccaaaaacgg gcgggcatgg aattttgttg cagggcagcg acggactata tgcaaaaaat
     3781 aggcttgata ataggaccgt ttgtaaggcg gaagagttgt ttaatttcga agggagtgat
     3841 gcgtaacggt ataccattta cacgaatatc aaacagaact tataaatgag gcaagaagac
     3901 atattttaaa gcataatgtg atgattgtta gtccacctgg aagtggtaag tcagtagtca
     3961 tttctgatat tgctaagtca gcgactcaaa aaaacggaca cgttttattc ttggtccatc
     4021 gcaaagaatt aattgaccaa atcactaata gttttaaatt ccatggaatt gacatgaata
     4081 aagtggattt aatgacagtt ggtaaagcta aaaaccgctt ggataaatta acaaaaccaa
     4141 ccttaattat cacagatgag ggtcatcatg ggaaagctag tacctaccaa ataatctatg
     4201 aatacttttc agacgtgccg aggattggtt ttactgccac accttggcgt ctatcaggcg
     4261 acggttttac agacacttac gatgttatgg ttcttggtaa aacggtcgag tggcttatca
     4321 ataataataa acttgcacca tatgattatt acagtgttct atcaattgat actgcgaaat
     4381 taaaagtaca aaacggagat tactccaata aatcgattga cgaatcattt ggtaaaaaga
     4441 tttttggtga tgtagttcaa gaatatataa aaaaagcgaa cggtcagaaa gctattttat
     4501 acgctcactc ggtagaagca tcgcaggcat ttgctaaaga attccaatct atgggaatta
     4561 atgcaataca cgcagatgca aaaacgccta aagccaaacg ggataaaatc atgaaagatt
     4621 tccgtgacgg taagatacaa gttatctgta acgttgattt gatatcagaa ggtttcgatg
     4681 tcccagattg tacagtaact attctttgta gacctacaaa atcattagta ttatttttgc
     4741 aacaatctat gcggtcgatg agatatcaac ctaataaaaa agccatcatt ttagatcacg
     4801 taggaaattg gaatattcac ggcttacctg acacaccgca tcattgggag aattatttcc
     4861 gaggagggtg gaagaagaag tcgaataaaa ctaacacggt acacgcaaaa gaatgttctg
     4921 tgtgttcggc tttgtggcca cttagtcaac agctctgtga attatgcaat catgattttg
     4981 gattgaaaga aaaacaagag aaagaacgca tagaggcaga acttgaactc ataaaacgtg
     5041 agcggtttag aatcaaacaa cttgctaata agaagtttgg taaagattta aaaacaaact
     5101 gggaaattgc ccaagctaga gttaaagacg ctggtaaagg aaaaccatta tataaactta
     5161 tctatttcta cttaaaaact gattgggtag aaacaaacgt taatgaactt gccgaagtaa
     5221 caggcaagtc agaaaaagaa atatatagcg cctacaattg gctaaaaaag aaattaagag
     5281 gataaaaaca tggcaggatt tacaacagat ttttcagaag ttaaagaaca cgcagaattc
     5341 aaagaacaac catacgaaat gattgtctat gatgcatatg aagcagtaaa tgaacgtaac
     5401 ggcaaaaaac gtgttgttat tgactacgtt gttcgaaacg atatcaagca agaaatgcaa
     5461 aactaccact tatgggatga gcaatatccc aactcacaaa ctgggaagta tcatatcggc
     5521 atcttaatgg gtaaagcaaa agcacttggt atcaaagagg ggcaacacta cgatagtttt
     5581 gaagcgtttt taaacgactt caaaggacgc actgcaaaag taaccgttaa acttgacgaa
     5641 tataacggaa ataaataccc gaaagttcgt tacgcaaatc aaagcgatgt gcctaacagc
     5701 taccacgtat ggaaagaaaa agcaactgga tttacacaag cggagattga agaagacgat
     5761 ctaccgttct aatttagact gggggataaa tgacatcaaa tgaatttatt gaagctttat
     5821 caaaattgac aaccgaaacc gattggggag acccaatctt cggcgagtca gtgcttaaag
     5881 ctgaattacg aaaacactta tttaaaattg tcccaattga tcacaatggg tatatccaca
     5941 aactgtttta ttcagaaatg gttaaagatg aagatgtcat gtattttgtg tcagatggac
     6001 gaaaaactta tcgcttttta tttggagata caattctaaa gactgataaa caaggcaatg
     6061 aatatctcac atactccgtt gaaaataatt ttccaccatt tgcaaaacta gttatcgact
     6121 acattctagg tgcttacacg ttttttgaga ataaacttta tgacattcga tataagcaat
     6181 ttaaattgat tgatgatttt acacttcaga ctaagtatgg tttcaaagac tctggtcaca
     6241 ttttagagat actacaaggt atccacaaaa cattaaacat ccaaccaatc aattatattg
     6301 aaccatatca gattgcttgc aaggatttca taatcgacct tgaaaattct gaaatcatta
     6361 accaaccgcc tttgcaaaac gtatcttatt ttaagtatta cgaagtagac tacaaaacag
     6421 caataaacag taagtctatt gcagaagaat atcttgagta cgttattgca gatagcaatt
     6481 cgttaaacaa tgcaatacta caatcttatt ttatcgccca agtagcgtgt ggtgttagac
     6541 ctaaaaccaa cttcttcatc tcaaaatctg gagtaaggac tggtaaaggg ttaaggcata
     6601 tagctttatc tggtctattc aataagattg atgttgagct agatacatta aaaagtaatg
     6661 gatttgaagc gttacaggcg tgggcgatgt tctcgggtgg agaaatggct ctagcgacgg
     6721 aacagggaga tattcaaggc aatgcaatgg agcgtgtact taaaattatt gcaacagaga
     6781 aaacacacgt tgcacgagca attgggcaaa atcagtcaat ttaatttaac gagtgttctg
     6841 tgtattgaca ccaaccgtac tgttgcgctg tcggatgaaa tgaatggacg aaaggtttta
     6901 attcagttta aagatagacc aaaaactgaa actgattatg aaagagagag catattcagg
     6961 aaatattggc tagcatttac tgactgtgat aaaaatccaa aaatcgatgg atgtatcggt
     7021 tttctgctaa attcactcga gcgtttccaa aaaattggta aatggtatca atggaaagat
     7081 gttgaagtat ttaatgatat cgatttagac gaatttcaag ttgctttaat aaatgcatta
     7141 caagaagttg attttgtaca gcgaactgat aacaaagaag ttattgactt atcattacaa
     7201 gtttacggaa aaagcaatca tgcattaagt aaagccatat ctgaaattgg tgtacgtagc
     7261 aggtcgaaga aagttaacgg gaaaacagtc agagggtatg aaatcgaaaa taaaacacgt
     7321 tttgataaat atatccttt
//
LOCUS       DATXQK010000060         7236 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.060, whole genome shotgun sequence.
ACCESSION   DATXQK010000060 DATXQK010000000
VERSION     DATXQK010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7236
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.060"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            584..673
                     /locus_tag="VC394_001531"
     CDS             584..673
                     /locus_tag="VC394_001531"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEP5994986.1"
                     /translation="MIIDYCNNKRIKIKRKGLSPLQYKTKSFT"
     gene            complement(711..1634)
                     /locus_tag="VC394_001532"
     CDS             complement(711..1634)
                     /locus_tag="VC394_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5994987.1"
                     /translation="MSEKLVEVKDLEISFGEGKKKFVAVKNANFFIKKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSSGQILYDGKVINGRKSKSEANELIRKIQMIFQDPAASLNE
                     RATVDYIISEGLYNFNLFKTEEERKEKIKNMMAEVGLLSEHLTRYPHEFSGGQRQRIG
                     IARALVMNPEFVIADEPISALDVSVRAQVLNLLKRMQAEKGLTYLFIAHDLSVVRFIS
                     DRIAVIHKGVIVEVAETEELFNNPIHPYTQSLLSAVPIPDPILERQKELVVYHPDQHD
                     YTLDKPSMVEIKPNHFVWANQAEIEKYQKEL"
     gene            complement(1627..2697)
                     /locus_tag="VC394_001533"
     CDS             complement(1627..2697)
                     /locus_tag="VC394_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003049688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5994988.1"
                     /translation="MTKENNVILTAKDVVVEFDVRDRVLTAIRNVSLELVEGEVLAFV
                     GESGSGKSVLTKTFTGMLESNGRIANGSIVYRGQELTDLKTNKEWAKIRGSKIATIFQ
                     DPMTSLSPIKTIGSQITEVIIKHQKVSHAKAKEMALDYMNKVGIPNAKKRFEDYPFEY
                     SGGMRQRIVIAIALACRPDILICDEPTTALDVTIQAQIVELLKSLQREYHFTIIFITH
                     DLGVVASIADKVAVMYAGEIVEFGTVEEIFYDPRHPYTWSLLSSLPQLADESGELYAI
                     PGTPPSLYSPIIGDAFALRSEYAMVLDFEKAPPAINVSETHWAKTWLLHPEAPKVQKP
                     EVIQDLHQKILRKMSQQEEGNV"
     gene            complement(2706..3632)
                     /locus_tag="VC394_001534"
     CDS             complement(2706..3632)
                     /locus_tag="VC394_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009569326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP5994989.1"
                     /translation="MESIDKSKFRFVERDSEASEVIDTPAYSYWKSVFRQFFSKKSTV
                     FMLVILVTVLMMSFIYPMFANYDFNDVSNINDFSKRYIWPNAEYWFGTDKNGQSLFDG
                     VWYGARNSILISVIATLINITIGVVLGAIWGVSKAFDKVMIEIYNIISNIPSMLIIIV
                     LTYSLGAGFWNLILAFCITGWIGVAYSIRVQILRYRDLEYNLASQTLGTPMYKIAVKN
                     LLPQLVSVIMTMLSQMLPVYVSSEAFLSFFGIGLPTTTPSLGRLIANYSSNLTTNAYL
                     FWIPLVTLILVSLPLYIVGQNLADASDPRSHR"
     gene            complement(3632..5134)
                     /locus_tag="VC394_001535"
     CDS             complement(3632..5134)
                     /locus_tag="VC394_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP5994990.1"
                     /translation="MKKYILNRIMRSLVSVVLVTALTYTIVYTLVPTSLIFKQDPNYN
                     KMTTTPDKKVAYENLTFQRMGYVNYFSSKELKDNASKVDSSVTTEATSANKAIYEKYI
                     DSLGNGWQLKRFPTSKQFYAIRNIPIYERVWNFFSNLVVIDHPWKIQDKDNPKLARYI
                     RLEKDKSVGWSLVGSGTKHKYLLYTNGKFPYLHQNFVTLNLGTSYPTYSNIPVLQVIS
                     QGQGRTALQDVTFPSGVTKKSSVDIYSRSYKNPKSLDDITKVNYGKGDSYTKTINNYV
                     DPSMIHNSFVIGFFGVMFSYIVGLPLGLFMARFKNTYFDRFSTATMTFMLALPSIAVI
                     YVVRFLGGMAGLPDSFPMLGASDPKSYILPALILGILNIPTTVIWFRRYLVDLQASDW
                     VRFARSKGLSESEIYRGHLFKNAMVPIVSGVPASIILAIGGATLTETVFAFPGMGKML
                     IDSIKSANNSMIVGLTFIFTVLSIVSLLLGDIVMTLVDPRIKLSTKKGGK"
     gene            complement(5199..7169)
                     /locus_tag="VC394_001536"
     CDS             complement(5199..7169)
                     /locus_tag="VC394_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEP5994991.1"
                     /translation="MKKSKWLAAVSVAILSVSALAACGNKNASGGSEATKTYKYVFVN
                     DPKSLDYILTNGGGTTDVITQMVDGLLENDEYGNLVPSLAKDWKVSKDGLTYTYTLRD
                     GVSWYTADGEEYAPVTAEDFVTGLKHAVDDKSDALYVVEDSIKNLKAYQNGEVDFKEV
                     GVKALDDKTVQYTLNKPESYWNSKTTYSVLFPVNAKFLKSKGKDFGTTDPSSILVNGA
                     YFLSAFTSKSSMEFHKNENYWDAKNVGIESVKLTYSDGSDPGSFYKNFDKGEFSVARL
                     YPNDPTYKSAKKNYADNITYGMLTGDIRHLTWNLNRTSFKNTKKDPAQQDAGKKALNN
                     KDFRQAIQFAFDRASFQAQTAGQDAKTKALRNMLVPPTFVTVGESDFGSEVEKEMAKL
                     GDEWKDVNLADAQDGFYNPEKAKVEFAKAKEALTAEGVTFPVQLDYPVDQANAATVQE
                     AQSFKQSVEASLGKENVIVNVLETETSTHEAQGFYAETPEQQDYDIISSWWGPDYQDP
                     RTYLDIMSPVGGGSVIQKLGIKAGQNKDVVAAAGLDTYQTLLDEAAAITDDNDARYKA
                     YAKAQAYLTDSAVDIPVVALGGTPRVTKAVPFSGGFSWAGSKGPLAYKGMKLQDKPVT
                     AKQYENAKEKWMKAKAKSNAKYAEKLADHVEK"
BASE COUNT         2251 a         1404 c         1346 g         2235 t
ORIGIN      
        1 ctttttttgt acttttttat ttgcccgcgt tgtaaaatga agtgcaacaa aaaagagaaa
       61 aagccttgaa ctacacccca aacgttagac aaaaaaatct aacgttcggg gtatttttta
      121 tgaaattaag tgatgaaagt acgaaataga catcgttaag aaagaaaaaa atacctacta
      181 tccaccagag ataaagcaag aaataatgga taacgttttg atagaaggcc cgccacaata
      241 ccaagtatca cttgtttatg gcttgttaca tgcaggaact ctcccaagtt gaatagtaca
      301 atataagaaa aacagttata ctgttgagaa aacaagagca agaccatcac aaacaggacg
      361 taaaccaaaa gaaacctgaa gaaatgacta aattagagcg gcttcaagaa gaaaatgaat
      421 atctcagaac tgaggttgct tacttaaaaa aaccgaggaa ctccgtttga aagaagcatc
      481 tctgtaaaga agaagacaaa aaaaccatta taaaaaatgg acaaagacga gttctgatta
      541 gattttcttt taagaacggc tgtattgact cgttctacct accattatca tcgattattg
      601 caacaataaa cgtatcaaaa taaaacgaaa aggacttagt cctctacaat acaaaactaa
      661 atcctttact taaaatagag tagccaactt ttggggtcag tgcatctctt ttacaattct
      721 ttttgatatt tttcaatttc tgcttggttt gcccaaacaa agtgatttgg tttgatttca
      781 accattgatg gcttatctaa tgtataatca tgttggtctg gatgatagac aacaagttct
      841 ttttgacgct ctaaaattgg atctgggata ggcacggctg ataacaaaga ttgggtgtag
      901 ggatgaattg ggttattaaa cagttcttct gtttctgcaa cttctacaat aaccccttta
      961 tggataaccg caatacgatc tgaaataaag cgaacgactg aaagatcatg ggcaatgaag
     1021 agataagtca aacctttttc ggcttgcatc cgtttgagaa ggtttaaaac ctgtgcgcga
     1081 acggaaacgt ccaaagctga aatcggttca tcagcaataa cgaattcagg gttcattact
     1141 agggctctag cgataccgat ccgctgacgt tgacctcctg aaaattcatg agggtagcgc
     1201 gtcaaatgct ctgatagcaa accaacttcg gccatcatgt tcttaatttt ttctttacgt
     1261 tcttcctctg ttttaaacag attaaagtta taaagacctt ctgaaatgat gtagtcaacg
     1321 gttgcccgtt cattcaaact agcagcggga tcttggaaaa tcatttgaat tttacgaatg
     1381 agctcattgg cttctgattt tgattttctg ccattaatta ctttcccatc gtataaaatt
     1441 tgacctgagc tagtatcgtt caaaccaata atagcacgac caattgttgt tttaccactc
     1501 ccagattctc caactaaaga aaaggtttct ccttttttaa taaagaaatt agcattttta
     1561 actgcaacaa attttttctt tccttctccg aaggaaattt ctaggtcttt tacttcgact
     1621 aatttctcag acatttcctt cctcctgttg tgacattttc cttaagattt tttgatgcaa
     1681 atcttgaatg acttctggtt tttgaacttt tggagcctct gggtgtaaaa gccatgtttt
     1741 ggcccaatga gtctcagata cgttaatcgc cggaggtgct ttttcaaagt ctaaaaccat
     1801 agcatattct gagcgaagtg caaaggcatc tccgataatt ggtgaataaa gtgatggagg
     1861 cgttcctgga atagcgtaaa gttcaccaga ttcatctgcc aactgcggta agctagacag
     1921 caaactccat gtataggggt gccttggatc atagaaaatc tcttcgactg ttccaaattc
     1981 aacaatttct cctgcataca tgacagccac tttatctgca atgcttgcca caacacctaa
     2041 atcgtgcgta ataaagataa tggtgaaatg atattctcgt tgaagcgact tcaataactc
     2101 aacgatttga gcttgaatag tcacatcaag ggctgttgtt ggctcatcac agataagaat
     2161 atctgggcga caagctaaag caatagcgat aacaatacgt tgacgcattc ctcctgaata
     2221 ctcaaatggg taatcttcaa agcgtttttt ggcatttggg atacccactt tattcatgta
     2281 atcaagggcc atttctttag ctttggcatg acttactttt tggtgcttaa taatcacttc
     2341 tgtgatttgg ctaccaatag ttttaatggg actaagactg gtcattgggt cttggaaaat
     2401 cgttgcgatt tttgagccgc gaatctttgc ccactcttta tttgttttta aatctgtcaa
     2461 ttcttgccca cgatagacaa ttgaaccatt agcaatgcgt ccattagact ccaacatccc
     2521 tgtaaaggtc tttgttaaaa cagatttacc tgagcctgat tcccctacaa aagcaaggac
     2581 ttctccttca acaagttcca gtgagacgtt acggatagct gttaaaacac gatcacgcac
     2641 atcaaattct accaccacat ctttggcagt taagattaca ttattttctt ttgtcatttt
     2701 aactcctatc tatgtgaacg tgggtcactg gcatcagcca agttttgtcc gacaatgtat
     2761 agtggtaacg atactaaaat caatgttact aagggaatcc aaaagaggta ggcatttgtt
     2821 gttaagttgc ttgaataatt agcaatcaaa cgtcctaaac ttggagtggt ggttggtaaa
     2881 ccaatcccaa agaaggataa gaaggcctca gaagatacat aaactggtag catttgtgac
     2941 aacatagtca tgataactga aaccaattga ggcaggaggt tcttaacagc aatcttgtac
     3001 attggtgttc ccaaagtttg actagcaagg ttgtattcta aatcacggta acgcaagatt
     3061 tgaacacgga tggagtaggc gacaccaatc catccagtga tacagaaagc tagaatcaaa
     3121 ttccaaaatc ctgcacctaa tgaataggtc aaaacaatga taataagcat agaagggata
     3181 tttgagataa tgttataaat ttcaatcata actttatcaa atgctttaga aactccccat
     3241 atggctccta acactacccc aatggtgata ttaattagtg tcgctataac tgagattaaa
     3301 atagaattac gtgccccata ccaaacacca tcaaacagag attgcccatt tttgtcggtt
     3361 ccaaaccagt actctgcatt tggccaaata taacgctttg aaaagtcatt gatattacta
     3421 acgtcattaa agtcgtagtt ggcaaacatt ggataaataa agctcatcat caagactgtc
     3481 actaaaatca cgagcataaa gactgtagat tttttagaaa aaaactgacg aaacactgat
     3541 ttccagtaag aataagcagg ggtatcaatc acttcggagg cttcactatc gcgctcaaca
     3601 aatcgaaatt tagatttatc aatcgattcc attacttacc tccttttttt gttgacaatt
     3661 taatacgtgg atcgacaagg gtcatgacaa tatctcctag taaaagtgaa acaattgaca
     3721 agacagtgaa gataaaggtt aatccaacaa tcatagaatt gttagcagat ttaatagaat
     3781 caattaacat tttacccata cccgggaagg caaagacagt ctctgttaag gttgcaccac
     3841 caatagcaag gataatactt gcaggaaccc ctgacacaat tgggaccatg gcatttttga
     3901 agagatgccc tctgtaaatt tcagattctg ataatccttt tgaacgcgca aaacgtaccc
     3961 aatcactggc ctgaaggtca acaagatagc gacggaacca gataaccgta gttggaatat
     4021 ttaaaattcc taaaataagg gctggtaaaa tataagattt aggatcagat gcccctagca
     4081 ttgggaaact atctggtaaa ccagccatgc cacctaggaa gcgaacaaca tagataacag
     4141 caatacttgg taaagcaagc atgaaggtca tggtcgctgt tgagaaacgg tcaaaatagg
     4201 tatttttaaa ccgagccata aataaaccaa gaggcaagcc aacgatatag gaaaacataa
     4261 caccaaaaaa accaattaca aaagaattgt gaatcattga tgggtcgaca taattattga
     4321 tggttttagt gtaggaatct cccttaccat agtttacctt ggtgatatca tccaatgatt
     4381 ttggattttt gtagctacgg ctatagatat ccactgacga tttcttagtg acacctgatg
     4441 ggaaggtcac atcttgaaga gctgttcgtc cttgcccttg tgaaataacc tgaagaacag
     4501 gaatattgct gtatgttggg taagatgttc ctaagtttag ggtaacaaag ttttggtgaa
     4561 gataaggaaa ttttccgtta gtgtagagga gatatttatg ttttgtcccc gaaccaacaa
     4621 gtgaccagcc aactgattta tctttttcta gacgaatata cctagctaat tttggattgt
     4681 ctttatcctg aatcttccaa gggtgatcaa taacaactag atttgagaaa aagttccaaa
     4741 cacgttcgta aataggaata ttgcgaattg cataaaactg tttgctagtg gggaagcgtt
     4801 ttagctgcca gccattgcca agagaatcaa tatacttctc atagatagct ttgttagcac
     4861 tagttgcttc tgtcgttaca ctactatcta ctttagaagc attatctttt aattctttgc
     4921 ttgaaaaata attgacataa cccatacgtt gaaaagttag attttcatac gctacttttt
     4981 tatctggtgt tgttgtcatt ttattataat tagggtcttg tttaaagata aggctggtag
     5041 gtactagagt atatacgatg gtgtatgtta aagccgttac caaaactacc gacactaacg
     5101 atcgcatgat acggtttaaa atgtattttt tcatagttat cctcaatagt tcaaaagaac
     5161 ttcctcacat aaacgagaaa gttcttttga acatcgtttt atttttcaac gtgatcagct
     5221 aacttctcag catattttgc atttgactta gcctttgctt tcatccattt ttcttttgca
     5281 ttttcgtatt gttttgctgt gacaggtttg tcttgaagtt tcattccttt atatgctaga
     5341 ggacctttag accctgccca agagaagccc ccgctaaatg gaacggcttt agtaactcgt
     5401 ggagtgccac ccaatgccac aactggaata tctacggcac tatctgtaag gtaggcttgt
     5461 gcttttgcgt aagctttata gcgcgcatcg ttgtcgtctg taattgctgc tgcttcatca
     5521 aggagagttt ggtaagtatc aaggcctgca gctgccacaa catccttatt ttgacctgct
     5581 ttgattccaa gtttttggat aacagatcca ccacctactg gactcatgat gtcaaggtag
     5641 gtccgtggat cttggtagtc tggtccccac catgatgaaa tgatatcgta gtcttgttgt
     5701 tctggggtct cagcatagaa gccttgggct tcgtgagttg atgtttctgt ttcaagaaca
     5761 ttgacaatga cattttcttt accaagagat gcttcaacag attgtttgaa agactgggct
     5821 tcctgaacag ttgctgcgtt tgcctggtca acagggtaat ctaattgaac tgggaaggtt
     5881 acaccttcag ctgtcaaagc ttctttggct tttgcaaatt caacttttgc tttttcagga
     5941 ttatagaaac catcttgagc atcagctaag ttaacgtctt tccattcatc accaagtttt
     6001 gccatttcct tttcaacttc tgaaccaaaa tcactttctc caacggttac aaaagttggt
     6061 ggcacaagca tgttacgtaa ggcttttgtt ttggcatctt gacctgcagt ttgtgcttgg
     6121 aatgacgctc ggtcaaaagc aaactgaata gcttgacgaa aatccttgtt gttaagagct
     6181 ttcttaccgg catcttgttg tgcagggtct ttcttagtgt ttttgaaaga agtacggttc
     6241 aaattccatg ttaaatgacg gatatctcca gtcaacattc cgtaagtaat attatcagca
     6301 tagtttttct tagctgattt gtaggtaggg tcatttgggt aaagtcgtgc aacgctgaac
     6361 tcacccttgt caaagttctt gtagaacgaa cctgggtctg aaccatctga gtaagtcaat
     6421 ttaacagatt ctatcccaac attcttagca tcccagtagt tttcattttt atggaattcc
     6481 atagatgatt ttgaggtgaa ggcgctcaag aagtaagcac cattaacaag gattgatgat
     6541 ggatcggttg taccaaaatc tttacctttt gacttcaaaa atttcgcatt aactgggaaa
     6601 agcacactat aagttgtttt tgaattccag tagctttcag gcttgttcaa agtatactga
     6661 acagttttat cgtcaagggc tttgacacca acttctttaa aatctacttc accattttgg
     6721 taagccttta agttttttat tgaatcttca acaacgtaaa gagcatctga tttatcgtca
     6781 accgcgtgct tcaaaccagt cacaaaatct tctgctgtta ctggggcata ttcttcacca
     6841 tcagccgtat accaagagac accatcgcga agagtataag tataagtcag accgtctttt
     6901 gaaaccttcc aatctttagc aagtgatggt actaaattac catactcatc gttttccaaa
     6961 agaccatcaa ccatttgtgt tatcacatca gtcgttccac cgccattagt caaaatataa
     7021 tccaatgatt ttggatcgtt aacaaaaacg tacttgtagg tttttgtagc ttctgagcca
     7081 cctgaagcat ttttattacc acaagctgcc aaagcggata ctgacaagat cgcaacactt
     7141 acagctgcca accatttact tttcttcata aaagtcaccc ctaaacttta tttttcattt
     7201 tttggcaatt gctaaatgaa attggttaat tgaaga
//
LOCUS       DATXQK010000061         7029 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.061, whole genome shotgun sequence.
ACCESSION   DATXQK010000061 DATXQK010000000
VERSION     DATXQK010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7029
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.061"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(110..1369)
                     /locus_tag="VC394_001537"
     CDS             complement(110..1369)
                     /locus_tag="VC394_001537"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEP5994992.1"
                     /translation="MRKIIINGGKALSGEVAVSGAKNSVVALIPAIILADDIVILDGV
                     PAISDVDSLIEIMELMGATVNYHGDTLEIDPRGVQDIPMPYGKINSLRASYYFYGSLL
                     GRFGQAVVGLPGGCDLGPRPIDLHLKAFEAMGVEVSYEGENMNLSTNGQKIHGAHIYM
                     DTVSVGATINTMVAATKAQGKTVIENAAREPEIIDVATLLNNMGAHIRGAGTDIITIQ
                     GVQKLHGTRHQVIPDRIEAGTYIALAAAIGKGVKITNVLYEHLESFIAKLEEMGVRMT
                     VEEDAIFVEKQESLKAITIKTSPYPGFATDLQQPLTPLLLKADGRGTIIDTIYEKRIN
                     HVPELMRMGADISVIGGQIVYQGPSRLTGAQVKATDLRAGAALVTAGLIAEGKTEITN
                     IEFILRGYASIIAKLTALGADIQLIED"
     gene            complement(1543..2739)
                     /locus_tag="VC394_001538"
     CDS             complement(1543..2739)
                     /locus_tag="VC394_001538"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEP5994993.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAEDPEAHVAAE
                     TCVYTGSVHVFGEISTKAYIDINRVVRDTIAEIGYTEAEYGFSAESVGVHPSLVEQSG
                     DIAQGVNEALESREGDTDDLSHIGAGDQGLMFGFAINETPELMPLPISLSHQLVRRLA
                     ELRKSGEISYLRPDAKSQVTVEYDEHDKPVRVDTVVISTQHDPEATNDQIRQDVIEKV
                     IKAVIPADYLDDDTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFSG
                     KDATKVDRSASYAARYIAKNLVAAGLATKAEVQLAYAIGVAQPVSVRVDTFGTSTVPE
                     AVLEAAVRQVFDLRPAGIIQMLDLKRPIYKQTAAYGHMGRTDIDLPWERLNKVDALVE
                     AVKTVL"
     gene            complement(3275..5653)
                     /locus_tag="VC394_001539"
     CDS             complement(3275..5653)
                     /locus_tag="VC394_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HEP5994994.1"
                     /translation="MKTKKVIILVGLLLSSQLTLISCQSRGNGTYPIKTKQSRKGMTS
                     NKIKPIKKSKKTNKTHKGMAGVDFPTDDGFILTKDSKILSKTDQGIVVDHDGHSHFIF
                     YADLKGSPFEYLIPKGASLAKPAVAQQAASQGTSKVADPHHHYEFNPADIVAEDALGY
                     TVRHDDHFHYILKSSLSGQTQAQAKQVATRLPQTSSPVSTATANGIPGLHFPTSDGFQ
                     FNGQGIVGVTKDSILVDHDGHLHPISFADLRQGGWAYVADQYDPAKKAEKPAETPHTP
                     ELSEREKEYQEKLAYLAEKLGIDPSTIKRVETQDGKLGLEYPHHDHAHVLMLSDIEIG
                     KDIPEPHAIEHARELEKHKVGMDTLRALGFDEEVILDIVRTHDAPTSFPSNEKDPNKM
                     KEWLATVIKLDLGSRKDPLQRKGLSLLPNLETLGIGFTPIKDISPVLQFKKLKRLLMT
                     KTGVTDYRFLDNMPQLEGIDISQNNLKDISFLSKYKNLTLVAAADNGIEDIRPLGQLP
                     NLKFLVLSNNKISDLSPLASLHQLQELHIDNNQITDLSPVSHKESLTVVDLSRNADVD
                     LATLQAPNLETLMVNDTKVSHLDFLKNNPNLSSLSINRAQLQSLEGIEASSVIVRVEA
                     EGNQIKSLVLKDKQGSLTFLDVTGNQLTSLEGVNNFTALDILSVSKNQLTNVNLSKPN
                     KTVTNIDISHNNIPLADLKLNEQHIPEAIAKNFPAVYEGSMVGNGTAEEKAAMATKAK
                     ESAQEASESHDYNHNHAYEDEEGHAHEHRDKDDHDHEHEDENEAKDEQNHAD"
     gene            5857..6798
                     /locus_tag="VC394_001540"
     CDS             5857..6798
                     /locus_tag="VC394_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEP5994995.1"
                     /translation="MKTSEKIYQLLSQTDDFVSGEYLADQLSISRTSVWKSIKSLENQ
                     GIQIDSLKHKGYRMVQGDILLPKTISQGLGMPVTYTPHSQSTQLDAKQGIEAHNSAPR
                     LYLAPSQEAAKGRLDRQFFSASTGGIYMSMYLKPNVPYADMPPYTMMVASSIVKAISR
                     LTGIDTEIKWVNDIYLGNHKVAGILTEAITSVETGLITDVIIGVGLNFFVTDFPEAIA
                     QKAGSLFTEKPTITRNDLIIDIWKLFLSIPVKDHVKVYKEKSLVLNKQVTFTENSQEK
                     RAIAIDLTDQGHLIVQFENGDFQTLRSGEISLSSWEN"
     gene            complement(6773..6976)
                     /locus_tag="VC394_001541"
     CDS             complement(6773..6976)
                     /locus_tag="VC394_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEP5994996.1"
                     /translation="MTIRQFLFMAFVCAFETYFFNDLLLSGNYLFALFWGLLLFRDLR
                     RVHTINQLTKTILKTANSPKKKD"
BASE COUNT         2084 a         1496 c         1254 g         2195 t
ORIGIN      
        1 tttatatttt ttatgacagt taaaaagcca taaaatgcaa caaagtatgc acctaagtat
       61 tattagccta agcacatgta atataaaaaa gagaagcgtt agcttctctt tagtcttcga
      121 ttaattgaat atctgcccct agtgctgtta attttgcaat gatactagca tagccacgta
      181 agataaattc aatattagta atttctgtct tgccttcagc tattagacca gcagtcacta
      241 gagccgctcc ggcacgtaaa tcagtggctt tgacctgagc tcctgtcagg cgagacggcc
      301 cttgataaac aatctgacca ccaataactg agatatctgc tcccatgcgc atcaactctg
      361 gcacatggtt aatgcgcttt tcatatatag tgtcaataat cgttccacga ccatctgctt
      421 ttaacaacaa gggcgttaat ggctgttgca aatcagtggc aaaaccaggg taaggagagg
      481 ttttaattgt aatagccttg agggattctt gcttttcaac aaagatagca tcctcttcta
      541 ccgtcatacg gacacccatt tcttctaact tagcaatgaa actctctaaa tgctcataaa
      601 gaacattagt gatcttaaca ccttttccaa tagctgccgc caaagcgata taagtccctg
      661 cttcaatacg atcaggaata acttggtgtc tagtaccatg caatttctga acaccttgta
      721 tcgtaataat atctgtacct gctccacgaa tatgcgcacc catgttattc aaaagagtag
      781 ccacatcaat aatctctggc tcacgtgcag cgttttcaat aaccgtttta ccttgagctt
      841 ttgttgccgc aaccatagta ttaattgtag caccgacact aacggtgtcc atatagatat
      901 gagcgccatg aattttttga ccatttgtcg ataaattcat attttcaccc tcataagaca
      961 cttcaactcc catagcctcg aaggccttca aatgcaagtc aattggtctt ggtcctaaat
     1021 cgcaaccacc aggtaagcca acaactgctt gtccaaaacg acctaaaaga ctaccgtaga
     1081 aataatagga agctcgaaga ctattaatct ttccgtaagg catcgggatg tcttgcaccc
     1141 ctctagggtc aatttctaaa gtatcaccat gatagtttac tgttgctccc ataagctcca
     1201 tgatttcaat cagactatct acatcactaa tagcaggtac accatcaaga atcacaatat
     1261 catcggctaa gatgattgct ggaataagcg caacaacact gttttttgcc cctgaaactg
     1321 ccacttctcc agataaggct tttccgccat taataataat tttacgcata cattcaaaac
     1381 tttcttatac tagaattcaa ccttttcatt ttatcataaa tcatgaagcc tgacaattat
     1441 tgacgtctta atttttaatc atccctttta aaggcttatt taataacaaa aaagcagagt
     1501 aagatttttt cactcactct acttttttaa taaagttgtc gtttataata ctgtttttac
     1561 agcttctact aaagcatcaa ccttatttaa acgctcccat ggcaaatcaa tatctgttct
     1621 ccccatgtga ccataggcag ctgtttgttt gtaaatcggg cgttttaaat caagcatttg
     1681 aataatgcct gctggccgta aatcaaagac ttgacgaaca gcagcttcta aaacagcttc
     1741 aggaaccgtt gaggttccaa atgtatccac gcgaacagac acaggctgag ccaccccaat
     1801 cgcataagct agttggactt cagctttcgt cgcaagacct gctgccacaa ggtttttagc
     1861 aatataacga gcagcataag aagccgaacg gtccactttt gtcgcatctt ttcctgaaaa
     1921 agcaccgcca ccatgacgtg aatagcctcc ataggtatcc acaataatct tacgtccggt
     1981 caatccagag tctccttgcg gacctccaat aacaaaacgt cctgttggat taatgaagaa
     2041 ctttgtgtca tcatctaaat aatctgctgg aattacagcc ttgatcactt tttcaatcac
     2101 atcctgacga atttggtcat tagtagcttc tggatcatgc tgagtagaaa taacgactgt
     2161 atccacacga acaggtttat cgtgttcatc gtactcaact gtcacttgag atttagcatc
     2221 tggacgtaga taactaatct cacctgactt acgtaattcc gccaagcgac gcactaattg
     2281 gtgagataag gaaattggta aaggcatcaa ttctggtgtt tcattgatgg caaaaccaaa
     2341 catgagccct tggtcacccg ctccaatatg gctaaggtca tcagtatcac cttctctgct
     2401 ttcgagagct tcattaaccc cctgagcgat atctccagat tgttctacaa gtgatggatg
     2461 tactcccact gattctgctg aaaagccata ttctgcttct gtataaccaa tttctgcaat
     2521 ggtgtcgcgg accacacggt taatatcaat gtatgctttt gttgagattt ctccaaaaac
     2581 atgaacagaa cctgtgtaaa cacaagtttc tgcagccaca tgggcttctg gatcttctgc
     2641 taaaatggca tctaaaatag catctgaaat ttggtctgca atcttatctg gatgcccttc
     2701 agatacggac tcagacgtga aaagtttacg ttctgacata aaaatgtccc ccctaatatg
     2761 ttgattcttc ctttttcagt cacattgacc acaaagcaag aagttgccta cccctttctt
     2821 cctagctgat tgagcaagtc aagtggccaa aaccaactta gctaatcagt tacggtttta
     2881 ctagtaaggc aactagctag tccacctagt ggctagcgtc cattaaccag acaaagaatg
     2941 tctgacaata gcgttgagtt acaaagaatc cggtttcctt gttgagagga tgtcaccaca
     3001 aaataagcct tagacgaagt aatttagcta aagagagaac gactgttccc caaatgaagt
     3061 caacaacgtc tcaaaacatt tcgttcaaca gaccgtctta tcgggactca ataacaatct
     3121 attataacat gttttagcca tttaggtgcc attcttttca ctcattatcc tatttatttc
     3181 aaaaaaacct caaggttatt cttgaggttt tcttgatagt caacaatggt gtcattaaga
     3241 cgattcagtt ggatcgggtt attcttagat tagtttagtc agcatggttt tgctcatctt
     3301 tagcttcatt ttcatcctca tgttcatggt cgtgatcatc tttgtctctg tgctcgtgag
     3361 catgaccttc ttcatcttca taggcatgat tatggttgta gtcatgtgat tccgatgctt
     3421 cttgagcact ttctttcgcc ttagtagcca tagctgcttt ttcttcagct gttccattac
     3481 ctaccataga accttcgtaa accgctggga agtttttcgc aatggcttct ggaatatgtt
     3541 gctcgttcaa tttaaggtct gccaatggga tattgttatg actaatatca atgttagtaa
     3601 ctgtcttatt gggtttagat aggttgacat ttgttaattg gtttttagac acgcttaaaa
     3661 tgtcaagtgc tgtaaaatta ttaacacctt ctagagaagt caactggttg cctgtcacat
     3721 ccaagaaagt aagtgaccct tgcttgtctt taagcacaag cgatttaatt tggttacctt
     3781 ctgcttctac tctgacaatg acgctacttg cttcaatacc ttcaagagat tgcaattgcg
     3841 cacggttaat agataggcta gatagattag gattattttt caagaaatcc aaatgagaaa
     3901 ccttggtatc attgaccatt aacgtttcta agttgggtgc ttgaagtgtt gctaagtcaa
     3961 catcagcatt tcttgataaa tcaacaaccg tcaatgattc tttatgagaa acagggctta
     4021 aatctgtaat ctgattatta tcaatgtgca attcttgcaa ttgatgtaac gatgccagtg
     4081 ggcttaaatc agaaatctta ttattactca atacgaggaa tttgagattt ggtaattgac
     4141 caagcggcct aatatcttca ataccattat cagcagccgc tactagagtt aagtttttat
     4201 atttgctcaa gaaactaata tctttgagat tgttttgtga aatatcaatg ccttctaact
     4261 gtggcatatt atccaaaaat ctataatcag tcacccctgt ttttgtcatt agtaaccgtt
     4321 tcaatttttt aaattgcaaa acaggtgaga tatctttgat tggtgtaaag ccaattccta
     4381 aagtttctaa gttgggtaac agtgaaagtc ctttacgttg caaaggatct ttacggctgc
     4441 ccaagtcaag tttgataacc gttgctaacc attctttcat cttattcgga tctttttcat
     4501 ttgatgggaa tgaggttgga gcatcgtgag tgcgaacgat atccaaaatc acttcttcat
     4561 caaaccctaa ggcacgcaag gtatccattc caaccttatg tttttccaat tcacgggcat
     4621 gctcaatagc atgtggttct ggaatgtctt ttccgatttc aatatcagat aacatcaata
     4681 cgtgtgcgtg gtcatggtga gggtattcca aaccaagttt accgtcttgt gtttccacac
     4741 gtttaatagt tgatggatca atccccaatt tttctgccaa ataagctaat ttttcttggt
     4801 attccttttc acgttcagat agctctggtg tgtgaggggt ttctgctggc ttttctgctt
     4861 ttttagcggg atcgtattga tctgccacat atgcccagcc accctgacga aggtccgcaa
     4921 aagaaatagg atgtaagtga ccatcgtggt ccactaaaat actgtctttt gttaccccaa
     4981 caataccttg accgttaaat tgaaaaccat ctgaggttgg gaaatgcaag cctggaatac
     5041 cattagctgt agctgttgaa acagggctac tggtttgtgg caagcgagta gcaacctgtt
     5101 tagcttgtgc ctgtgtctga cctgataagc ttgacttcaa aatataatgg aagtgatcat
     5161 cgtggcgaac cgtgtagcct aaagcatctt cagccacaat atccgctggg ttaaattcat
     5221 aatggtgatg aggatctgct actttagaag tcccttgact agctgcttgc tgagcaacag
     5281 ctggcttagc taaacttgct ccttttggaa taaggtattc aaatggactt ccctttaaat
     5341 cggcataaaa aataaaatgc gaatgaccat catggtcaac aacgattccc tgatctgttt
     5401 ttgataagat ttttgagtct ttggttaaaa taaacccatc atctgtagga aaatcgacac
     5461 ccgccatacc tttgtgagtc ttgtttgtct ttttgctttt tttaatcggt ttaattttgt
     5521 ttgacgtcat tcccttacgt gattgtttcg ttttaatggg atatgtacca ttacctcgtg
     5581 attgacaaga tatcaaagtc aactgagatg ataacaatag accaactaaa ataataactt
     5641 ttttcgtttt cattcaaccc tcctttttat ttaactggtt aagtatatca catcacttgt
     5701 ttttcctcaa gctcttttta ggataactat tagttgttga cgagttggag ctctgaagct
     5761 tcaacaaaca atagtttctg ttgttagtag cattacacaa aaaaactaac ttttcttttc
     5821 ctttgaaaat aagatctttt ataatatact atacatatga aaacatcaga aaaaatttat
     5881 caattactgt cacaaaccga tgacttcgtc agtggggaat atctagcaga tcaactcagt
     5941 atctctcgta catccgtttg gaaatccatc aaaagtctcg aaaatcaggg gattcaaatt
     6001 gattctttaa aacacaaggg ctaccgcatg gtacaaggtg atattttatt acccaaaacc
     6061 attagccaag ggcttgggat gccagtcacc tatacccctc acagccaatc tacccaatta
     6121 gatgccaaac aaggaatcga agctcataac agcgctccaa ggctttacct agctcctagc
     6181 caagaagctg ccaaaggacg cctcgatcgg caattttttt cagctagcac cggtggtatt
     6241 tacatgtcca tgtacctaaa acccaatgtc ccttatgctg atatgcctcc ttatacgatg
     6301 atggttgctt ccagtattgt caaagctatc tcgcgattaa caggcattga tacagaaatc
     6361 aaatgggtaa acgacatcta tctaggtaac cataaagttg cagggattct gacagaagcc
     6421 attacttctg ttgaaactgg tttaatcacc gatgtcatta ttggggttgg tctcaacttt
     6481 tttgtcaccg acttcccaga agcgattgct caaaaggcag gttctctctt taccgaaaaa
     6541 ccgactatca cgcgcaatga ccttatcatt gacatctgga aattattctt atctattcct
     6601 gtcaaggacc atgtcaaagt ttacaaagaa aaatcacttg ttcttaataa acaagtgacc
     6661 tttacagaaa atagtcaaga aaaacgtgct attgcgattg atctcacaga tcaaggacac
     6721 ctcattgtac agtttgaaaa tggtgacttc caaaccttgc gaagcggtga aatcagtctt
     6781 tcttcttggg agaattagcc gtttttaaaa tggttttagt caattggtta atggtatgca
     6841 ctcttcttaa atccctaaag agcaacaagc cccaaaataa ggcgaagaga taatttccag
     6901 agagcaacag gtcattaaaa aaataggttt caaaagcaca tacaaaggcc ataaaaagaa
     6961 attgtcgaat agtcatagct ttattataac agaaaaaaat gattctatga agaatccaca
     7021 cagattgat
//
LOCUS       DATXQK010000062         7017 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.062, whole genome shotgun sequence.
ACCESSION   DATXQK010000062 DATXQK010000000
VERSION     DATXQK010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7017
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.062"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(27..299)
                     /locus_tag="VC394_001542"
     CDS             complement(27..299)
                     /locus_tag="VC394_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HEP5994997.1"
                     /translation="MSDAFTDVAKMKKIKEDIRAHEGQLVELTLENGRKREKNKIGRL
                     IEVYSSLFIIEYSDSSDTPGAIDNSYVESYTYSDILTEKTLIRYLD"
     gene            complement(316..1683)
                     /gene="dnaB"
                     /locus_tag="VC394_001543"
     CDS             complement(316..1683)
                     /gene="dnaB"
                     /locus_tag="VC394_001543"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEP5994998.1"
                     /translation="MRLPEVAELRVQPQDLLAEQSVLGSIFISPDKLIAVREFISPDD
                     FYKYAHKIIFRAMITLSDRNDAIDATTIRTILDDQDDLQSIGGLSYIVELVNSVPTSA
                     NAEYYAKIVAEKAMLRDIIARLTESVNLAYDEILKPEEVIAGVERALIELNEHSNRSG
                     FRKISDVLKVNYEALEARSKQTSNVTGLPTGFRDLDKITTGLHPDQLVILAARPAVGK
                     TAFVLNIAQNVGTKQKKTVAIFSLEMGAESLVDRMLAAEGMVDSHSLRTGQLTDQDWN
                     NVTIAQGALAEAPIYIDDTPGIKITEIRARSRKLSQEVDGGLGLIVIDYLQLITGTKP
                     ENRQQEVSDISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDA
                     DIVAFLYRDDYYRKEGDDAEEAVEDNTIEVILEKNRAGARGTVKLMFQKEYNKFSSIA
                     QFEER"
     gene            complement(1713..2165)
                     /gene="rplI"
                     /locus_tag="VC394_001544"
     CDS             complement(1713..2165)
                     /gene="rplI"
                     /locus_tag="VC394_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEP5994999.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATSQSI
                     GELKGKQKAEEKAQAEILAEAQAVKAVLDEDKTRVQFQEKVGPDGRTFGSITAKKISE
                     ELQKQFGVKVDKRHIVLDHPIRAIGLIEVPVKLHKEVTAEIKLTITEA"
     gene            complement(2162..4138)
                     /locus_tag="VC394_001545"
     CDS             complement(2162..4138)
                     /locus_tag="VC394_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003045829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEP5995000.1"
                     /translation="MKKFRFETIHLIMMGLILFGLLALCVSIMQSKILILLAIFLVLL
                     FVVALLWYQKEAYHLSDLAHIELLNEQTEDNLKTLLDNMPVGVVQFDQETNAVEWYNP
                     YAELIFTTEEGFIQNSLIQQIITEKRREDISQTFEVSGNKYTSYIDVSSGIFYFFDSF
                     VGNRQLADASMLRPVVGIISVDNYDDITDDLSDADTSKINSFVANFIDEFMESKRIFY
                     RRVNMDRYYFFTDFKTLNDLMDNKFSVLEEFRKEAQDAQRPLTLSIGISFGEENHSQI
                     GQVALENLNIALVRGGDQIVIRENADHTNPIYFGGGSVSTVKRSRTRTRAMMTAISDR
                     IKMVDNVFIVGHRKLDMDALGSAVGMQFFAGNIIENSFAVYNPDEMSPDIERAIERLQ
                     ADGKTRLISVSQAMGLVTPRSLLVMVDHSKISLTLSKEFYEQFQNVIVIDHHRRDDDF
                     PDNAILTFIESGASSAAELVTELIQFQNAKKRLNKIQASVLMAGIMLDTKNFSTRVTS
                     RTFDVASYLRSKGSDSVEIQNISATDFEEYKQINEIILQGERLGDSIIVAAGEKNHLY
                     SNVIASKAADTILSMAHVEASFVLVETASHKIAISARSRSKINVQRVMEKLGGGGHFN
                     LAACQLTDISLPQAKHLLLKTINMTMKETGEVES"
     gene            complement(4228..6126)
                     /gene="mnmG"
                     /locus_tag="VC394_001546"
     CDS             complement(4228..6126)
                     /gene="mnmG"
                     /locus_tag="VC394_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEP5995001.1"
                     /translation="MTHEFTESYDVIVIGAGHAGVEASLATSRMGCKTLLATINLDML
                     AFMPCNPSIGGSAKGIVVREIDALGGEMGKNIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KSLYAREMKHTVEKQANLTLRQTMIDDILVEDGRVVGVLTATGQKFAAKAVVVTTGTA
                     LRGEIILGELKYSSGPNNSLASVTLADNLKKLGLEIGRFKTGTPPRVKASSINYDQTE
                     IQPGDDKPNHFSFMSKDAEYLKDQIPCWLTYTNQTSHDIINQNLYRAPMFSGIVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRDTEEVYVQGLSTSLPEDVQKDLIHSIKGL
                     EKAEMMRTGYAIEYDIVLPHQLRATLETKLISGLFTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKPELILKRSDAYIGVMIDDLVTKGTLEPYRLLTSRAEYRLILRHDNADMR
                     LTEIGRDIGLVDDERWKAFEIKKNQFDNELKRLNSIKLKPVKATNDRVQELGFKPLTD
                     AMTAKEFMRRPEIDYATAVSFVGPAAEDLDAKIIELLETEIKYEGYIRKALDQVAKMK
                     RMEEKRIPANIDWDAIDSIATEARQKFKKINPETIGQASRISGVNPADISILMIYLEG
                     NGKAHRKY"
     gene            complement(6250..6567)
                     /locus_tag="VC394_001547"
     CDS             complement(6250..6567)
                     /locus_tag="VC394_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEP5995002.1"
                     /translation="MIVRNGKNFLTRDAADQYYTIGGTSLVGEKTHETVLRETLEEVG
                     IRTKVNQLAFVVENHFDIDDVFWHNIEFHYLVSPLEDPKLEMIENASDRFVNGFQSIN
                     WIK"
BASE COUNT         2145 a         1476 c         1086 g         2310 t
ORIGIN      
        1 aagaaaaagc ctctatgagg caaaaatcaa tctaaataac gaattaatgt tttttctgtc
       61 aaaatatctg aataggtgta tgattcgaca taagaattat caattgctcc tggagtatca
      121 gagctatcac tatactcaat gataaagagc gaactgtaaa cctcaataag cctaccaatt
      181 ttattttttt cccgtttccg tccattctca agggttaatt ctactaactg tccttcatgg
      241 gctcttatgt cctctttaat ttttttcatt tttgcaacat ctgtaaatgc atcgctcatt
      301 ttttgtctct ctttcttatc tttcttcaaa ctgggctata cttgagaatt tgttgtattc
      361 tttttgaaac atcagtttga ctgttccacg cgccccagct ctatttttct cgaggataac
      421 ttcaattgtg ttatcttcaa cagcttcttc agcatcatca ccttctttac ggtaataatc
      481 gtcccggtat aagaaggcta caatatcggc atcctgctca atagatcctg attcacgaat
      541 atctgataaa actggtcgtt tatcttgcct ttgctcaacg ccacgagaaa gctgacttag
      601 ggcaataact ggtactttca attctttagc taggatttta agctgtcttg aaatatctga
      661 aacctcttgc tgacgatttt cgggtttagt tcctgtaatc aactgtaagt agtcaattac
      721 gataagacct aaaccaccat ccacttcttg agacaatttc cgtgatcttg cgcggatttc
      781 agtaatttta atcccgggcg tatcgtcaat ataaatcggt gcttctgcca aagctccctg
      841 agcaattgtt acattattcc aatcctgatc tgtgagttgc cctgttctta aactgtgcga
      901 atcaaccatt ccttctgctg caagcatacg atctactaaa ctttcagcac ccatttccaa
      961 agaaaaaata gcaacagtct ttttttgctt agtccccaca ttttgcgcaa tattaagaac
     1021 aaaggcagtc ttccccactg ctggccgagc agctaaaata actaattgat ctgggtgtaa
     1081 acctgttgta atcttgtcaa ggtctctaaa accagttggt aaacctgtaa catttgaagt
     1141 ctgcttagaa cgtgcttcta aagcctcgta attaactttt agcacatctg aaattttgcg
     1201 aaacccacta cgattactat gttcattgag ttcaattaaa gctctctcaa ctccagcgat
     1261 aacctcttct ggttttaaaa tttcatcata agctaggttg acagattctg tcaacctagc
     1321 aataatatca cgcaacatag ctttctcagc tacaatttta gcataatatt ctgcattagc
     1381 actagttggg acactattaa ctagttcaac aatataggat aagccaccaa tactttgcag
     1441 atcatcttga tcatctagga ttgttcttat agtggttgca tcaatggcat cattacgatc
     1501 gctgagggta atcattgccc gaaagataat tttatgagcg tacttataaa aatcgtctgg
     1561 actgataaat tctctcactg caatcagctt atcaggtgag ataaagattg acccaagaac
     1621 agattgctct gctagtaaat cttggggttg aactcgtaat tcagctactt caggcaacct
     1681 cataacctcc aaatcttgtg atgtttttta tattatgctt ctgtgatagt caatttaatc
     1741 tcagctgtta cttccttatg taatttcaca ggaacttcaa ttagaccaat ggcgcggata
     1801 ggatgatcta aaacaatatg acgtttatca acttttacac caaattgctt ttgcaattct
     1861 tctgaaatct ttttagctgt gatggaacca aatgtacgtc catctggacc aactttttct
     1921 tgaaattgaa cacgtgtttt gtcctcatcc aaaacagcct tgacagcttg agcttctgct
     1981 aatatttcag cttgagcttt ttcttcagct ttttgttttc cttttaattc accgatgctt
     2041 tgactagttg cttctttagc taaatttttc ttaataagga aattttgagc atagcctgtt
     2101 gggacctctt tgatttctcc ttttttacct tttcctttta catctgctaa aaaaataact
     2161 ttcatgattc tacttctcct gtttctttca ttgtcatatt aatagttttc aataataagt
     2221 gcttcgcttg gggaagactg atatccgtta actgacaggc agcaagatta aagtggcctc
     2281 ctccacctaa tttttccatc acacgttgaa cattgatttt actacgactt ctagctgaaa
     2341 tagcaatctt atgagaagct gtttcaacta atacaaagct agcctcaacg tgagccattg
     2401 acagtatcgt atctgcagct ttactagcaa taacattgct ataaagatga tttttttctc
     2461 ctgcagctac aatgatactg tcaccaagac gttctccttg taaaataatt tcatttattt
     2521 gcttatattc ttcaaaatct gttgcggata tattttgaat ttcaacacta tcacttcctt
     2581 tgcttcttag gtaactagcg acatcaaagg tacgacttgt cactcgcgta gaaaaatttt
     2641 tagtatcaag cataatacct gccattaaaa cactggcttg gatcttattt aaacgttttt
     2701 tagcattttg aaattgaatt aactcagtga cgagctccgc agcactactt gctccacttt
     2761 caataaaagt taagatagca ttatcaggaa aatcatcatc tcttctgtga tgatcaatta
     2821 caataacatt ttgaaactgt tcataaaatt ctttagaaag tgttaaagat atcttagaat
     2881 ggtcaaccat tactaaaagt gatcttggag tcactaaacc cattgcttga gaaacactga
     2941 ttagacgagt ctttccgtca gcctgcaatc gctcaatagc cctttcaata tctggactca
     3001 tctcatctgg attgtaaacg gcaaaactat tttcaataat gttacctgcg aaaaattgca
     3061 taccaacagc tgaacctagt gcatccatat caagtttcct atgtccaaca ataaaaacat
     3121 tgtccaccat cttgattcga tctgaaatag ctgtcatcat agcacgggta cgtgttcttg
     3181 aacgtttaac tgtagaaaca gaccctcccc cgaaataaat tggattcgta tgatccgcat
     3241 tttcacgaat gacaatctga tcaccaccac gaacaagcgc aatattaaga ttctcaagag
     3301 ctacctgtcc aatctggcta tgattttctt caccaaaaga aattcctatg ctgagtgtta
     3361 aagggcgttg agcatcttga gcttctttac gaaactcttc taagacagaa aatttattat
     3421 ccatcaaatc atttaatgtt ttaaagtctg taaagaaata gtagcgatcc atattaacac
     3481 gacggtaaaa aatccgtttt gactccataa actcatcaat gaagttagct acaaatgaat
     3541 taatttttga ggtatctgca tctgataaat catcagtgat atcatcataa ttatcaaccg
     3601 aaataatgcc aacaaccggt cttaacatac tagcatcagc taattgcctg tttcccacaa
     3661 agctatcaaa aaaataaaaa attcctgatg atacatcaat ataagaagtg tatttattac
     3721 cagaaacttc aaatgtttga gaaatatctt cacgacgttt ttccgtaata atttgctgaa
     3781 tcaaactgtt ttgtataaaa ccctcttcag ttgtaaaaat taattctgca taaggattat
     3841 accattctac agcgttggtc tcttgatcaa attgaactac tccaacaggc atattatcaa
     3901 gtagcgtttt taggttatct tctgtttgtt catttaagag ctcaatatga gctaagtctg
     3961 ataagtgata agcttctttt tgataccata aaagagcaac aacaaatagc aaaacgagaa
     4021 aaatagctaa taatattaat atttttgatt gcataatact gacacaaagt gccagtagtc
     4081 caaataaaat caatcccatc ataatcaaat gaatggtttc aaaacgaaat tttttcatct
     4141 tataacctct taagcacaca ttataccata aaaaagccct gaaatacaga gcttccactt
     4201 gctttgagat tacaatttca tttccattta atattttcga tgagcttttc cgtttccttc
     4261 taaataaatc atcaagatac tgatgtccgc aggattgact cctgaaatac gactagcttg
     4321 tccaattgtt tcaggattaa ttttcttaaa tttctgacgc gcctcagttg caatagaatc
     4381 aatagcatcc caatcaatat ttgcaggaat acgtttttct tccatgcgtt tcatcttagc
     4441 aacctgatct aaagctttgc gaatatagcc ttcgtacttg atttctgttt ctaaaagttc
     4501 aatgatttta gcatctaaat cttcagccgc aggaccaaca aaagagactg cagtcgcata
     4561 atcaatttct gggcgacgca taaattcttt agctgtcata gcatccgtca atggtttaaa
     4621 accaagttcc tgcacacgat cgttggttgc ttttactggt tttaatttga tactgttcag
     4681 acgttttaat tcgttatcaa attggttttt cttaatttcg aaggccttcc aacgctcatc
     4741 atcaaccaac ccaatgtcgc gaccaatctc tgtcaaacgc atatcagcgt tgtcatgacg
     4801 taaaatcaaa cgatactcag ctcgcgatgt caacaatcga taaggttcca aggttccttt
     4861 ggtaaccaaa tcgtcaatca taacgccaat ataagcatcg ctccgtttca aaataagttc
     4921 tggcttacct tgaactttca aagcagcatt gattccagca ataagtcctt ggccagcagc
     4981 ctcttcatac ccagaagttc cattagtttg cccagccgta aagagtcctg aaattagctt
     5041 ggtttcaagt gtggcacgta gctggtgagg taaaacaata tcatattcaa tagcataacc
     5101 agtgcgcatc atttcagcct tttcaagtcc tttgatcgaa tgaatcaaat ctttttggac
     5161 atcttctgga agactcgttg acaacccttg aacatacact tcttctgtat cacgcccctc
     5221 tggctctaag aaaagttgat gacgttcttt atcagcaaag cgaacaatct tatcttcaat
     5281 agatgggcag taacgagggc ctacaccttt aacaatgcca gaaaacatcg gtgctctgta
     5341 aaggttctga ttaatgatat catgactagt ttgattcgta taagtcaacc agcagggaat
     5401 ttgatctttg agatactcag catcttttga cataaatgaa aaatgattag gtttgtcatc
     5461 acctggctga atttccgttt gatcgtaatt aatagaagat gctttgacac gaggaggcgt
     5521 tcctgttttg aatctgccaa tttctaagcc gagttttttt aaattatctg ctaaagttac
     5581 tgatgccaaa ctattattag gcccagaaga atatttgagt tctcctaaaa taatttcgcc
     5641 acgcagagct gttcctgttg tcactacaac tgccttagct gcaaattttt gtcctgtggc
     5701 tgttaaaaca cctacaactc gtccatcttc aactagaata tcatcaatca tcgtttggcg
     5761 aagagtcaaa ttagcctgtt tttcaaccgt atgtttcatt tcacgcgcat acagggattt
     5821 atctgcttga gctcggagag cgcgtacagc tggtccctta cctgtattaa gcattttcat
     5881 ctggatatag gttttatcaa tatttttacc catttcgccg ccaagagcat caatctctct
     5941 aaccactatc cctttggcag atcctccaat agaaggatta cacggcataa aagccaacat
     6001 atcaaggttg attgttgcca ataaagtttt acaacccata cgactagtgg ccaaactggc
     6061 ttcaacacca gcatgacctg cacctatgac aatgacgtca tagctttctg taaattcatg
     6121 tgtcattttt ttaaaatcct ttatttcagt ctattcattt aaaatcatac gctttagttt
     6181 agccttcaat gcggacaata tgtcctggcc atttagccaa ctcggtttgt aaaaactcag
     6241 gaacaagatt catttgatcc agttgattga ttggaatcca ttcacaaacc tgtcgcttgc
     6301 attctctatc atttctagtt tagggtcttc taaggggctt accaaataat gaaattcaat
     6361 attatgccag aaaacatcat cgatatcaaa atgattctca accacaaaag ccagttgatt
     6421 aaccttagtt cgaataccaa cttcctctaa ggtttcacgt aaaacagttt catgcgtttt
     6481 ttcacctacc aaacttgtac caccaatggt gtaatattgg tcagctgcat ctctagttaa
     6541 aaaattctta ccatttctaa caatcaagca ctagctcgta aactaaagtt atctgacctc
     6601 tagtgattct aaatcccagt cctattagcc atctcaattc tagagctaaa ccataaaaag
     6661 ggccaaaacc ctagaagatt gcaggacaaa aaaacctaca actttcatga gctttgacca
     6721 tcattccgat tgttattatc tattttaaaa aaatgattag tgattgtcaa tattgatttc
     6781 atcacagacc tttacagtaa tgtcaatgtt tttacatctc tgtcaatcta ttgtaatgtt
     6841 tttaaaatga ttacaaaagt atcactatgt ctaatctagc gaggcgagaa ataaaaatgt
     6901 catcagtatt atcatacgat tttttgatat cttctaaagt agtaaaatac agataccgtt
     6961 gtttctaagt ctacaagtaa gaacgccgat aacaaaacca taaaagtata actagaa
//
LOCUS       DATXQK010000063         6738 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.063, whole genome shotgun sequence.
ACCESSION   DATXQK010000063 DATXQK010000000
VERSION     DATXQK010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6738
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.063"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..949
                     /locus_tag="VC394_001548"
     CDS             <1..949
                     /locus_tag="VC394_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEP5995003.1"
                     /translation="NTLTISKIVYQKKFFHLAKKSEKVISTYHVTDDLKPFQVRDLVS
                     GHLERIQEEVEKKYPNAGFNSDKYNGLKESNSLLSDGFEVKSGNLIFDKKLTIPLTTL
                     FDVINPDFLANSDRAAYDNYRTYKEQHPKKLVALTFDDGPDPTTTPQVLDILAKYQAK
                     GTFFMIGSKVVNNENLTKRVSDAGHEIANHTWDHPNLTNLSVSEIQHQVNMTNQAIEK
                     ACGKKPRYLRPPYGATNATVQQSSGLTQMLWTVDTRDWENHSTDGIMTNVKNQLQPGG
                     VVLMHDIHQTTINALPAVMEYLKAEGYECVTVSELYAHQ"
     gene            complement(1059..2144)
                     /locus_tag="VC394_001549"
     CDS             complement(1059..2144)
                     /locus_tag="VC394_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEP5995004.1"
                     /translation="MITKFPPQWQEKLDQVAFTHLTPIQEQAFQPIVDGKNFLGISPT
                     GTGKTLAYVFPSLLALTPKKSQQLLILAPNTELAGQIFEVTKDWAQPLGLTAQLFISG
                     TSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMSVNTIVLDEYDELLGDSQYDFV
                     QKISHYVPRDHQMVYMSATNKVDQTSLAPNTFCIDLSEQTNDAIQHFYLMVDKRERTD
                     LLRKFANIPHFRALVFFNSLSDLGATEERLQYNGAAAVSLASDINVKFRKTILEKFKS
                     HQLSLLLATDLVARGIDIDNLDYVIHFDVARDKENYTHRAGRTGRMGKSGIVITFVSH
                     PEDLKKLKKFAKVSEISLKNQQLHFIN"
     gene            2242..2868
                     /gene="udk"
                     /locus_tag="VC394_001550"
     CDS             2242..2868
                     /gene="udk"
                     /locus_tag="VC394_001550"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEP5995005.1"
                     /translation="MLKKPIIIGVTGGSGGGKTSVSRAILDSFPNARIAMIQHDSYYK
                     DQSHMSFEERVKTNYDHPLAFDTDFMIQQLKELLAGRPVDIPIYDYKKHTRSNTTFRQ
                     DPQDVIIVEGILVLEDERLRDLMDIKLFVDTDDDIRIIRRIKRDMMERGRSLESIIDQ
                     YTSVVKPMYHQFIEPSKRYADIVIPEGVSNVVAIDVINSKIASILGEV"
     gene            2948..3211
                     /locus_tag="VC394_001551"
     CDS             2948..3211
                     /locus_tag="VC394_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEP5995006.1"
                     /translation="MLLIWLGLLFVWNELVFVIYAFDKRKAIKKKRRISERNLLVITV
                     LFGGFGALLAAKKYHHKTRKWYFVITWYTSILLTLLVTYIFMK"
     gene            complement(3264..4073)
                     /locus_tag="VC394_001552"
     CDS             complement(3264..4073)
                     /locus_tag="VC394_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEP5995007.1"
                     /translation="MKQITQQKWLRYGLFIALVLNGIELELLGLTANNLSFKEAFALI
                     LTISLLGIYLIPFAAAIFYLSKKFHMNLNVIIVSCLSGLYISGFLASCGNHLVGQFWS
                     YIIPSKDALKLWGDALTAPIVEEPIKASSAILVITLFPRLTLKEKLVVALLSGMGFQL
                     AEDIRYLIQAKSIDSLVPTAIERISTAVTSHWVHTAIFTIGAYLLLKGSNLFSKQQQI
                     FWLLSPLVLHFIWNSPLTSIPGMTVLLGTLILLIFGDLFQKINTLDDDVLF"
     gene            4218..4715
                     /locus_tag="VC394_001553"
     CDS             4218..4715
                     /locus_tag="VC394_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012678242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEP5995008.1"
                     /translation="MNKSKKIEQYQLMIAQAKELFANESNALANLSNASALLNMTLPN
                     SVFAGFYLFDGQELILGPFQGRVSCVHIKLGKGVCGESAQSRRTIIINDVKQHANYIS
                     CDAAAMSEIVVPMVKEGHLIGVLDLDSSLVADYDEVDQEYLEAFVDLFLEKTTFTFNM
                     FGVKN"
     gene            4715..6400
                     /gene="dnaX"
                     /locus_tag="VC394_001554"
     CDS             4715..6400
                     /gene="dnaX"
                     /locus_tag="VC394_001554"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEP5995009.1"
                     /translation="MYQALYRKYRSQTFDEMVGQSVISTTLKQAVESGKISHAYLFSG
                     PRGTGKTSAAKIFAKAMNCPNQVDGEPCNQCDICRDITNGSLEDVIEIDAASNNGVDE
                     IRDIRDKSTYAPSRATYKVYIIDEVHMLSTGAFNALLKTLEEPTENVVFILATTELHK
                     IPATILSRVQRFEFKAIKQKAIREHLAWVLDKEGIAYEVDALNLIARRAEGGMRDALS
                     ILDQALSLSPDNQVTIAIAEEITGSISMLALGDYVRYVSQEQATQALAALETIYDSGK
                     SMSRFATDLLTYLRDLLVVKAGGDNQRQSAVFDTNLSLSIDRIFQMITVVTSHLPEIK
                     KGTHPRIYAEMMTIQLAKEEGVALPSGLSSDVTTEVEGLKRELNQLKQALAQLQASPS
                     PSHAPVKPAQAKPKLTTYKIDRVKILKIMEETVQDSQQSRQYLDALKTAWNEILDSIS
                     AQDRALLMGSEPVLANSENAILAFEAAFNAEQAMKRTDLNAIFGNIMSKAAGFSPNIL
                     AVPKADFHHIRSEFALQLKTQKASDPTEETIEETLEIPEGFDFLVDKINAVED"
BASE COUNT         2180 a         1129 c         1375 g         2054 t
ORIGIN      
        1 taacactttg actatttcta aaatagttta tcagaaaaaa ttttttcatt tggctaaaaa
       61 atcagaaaaa gttataagta catatcacgt tacagacgac ttgaaaccgt ttcaggtaag
      121 ggatctagta tcaggacatt tagaaagaat acaagaagaa gttgaaaaaa aatatccaaa
      181 tgctggtttt aatagcgata agtacaatgg cttaaaagaa tctaattctt tattaagcga
      241 tggctttgag gtaaaatcgg gaaaccttat ttttgataaa aagctaacga tacctttgac
      301 gacattattt gatgttatta atccagattt tttagcaaat agcgatagag ctgcgtatga
      361 taattatagg acctacaaag aacagcatcc caaaaaacta gttgcattaa cgtttgatga
      421 tggtccagat ccgacgacga ctcctcaagt tttagatatt ttggcaaaat accaggctaa
      481 gggaactttc tttatgatag gttcaaaagt tgtgaataat gaaaacctta ctaaacgtgt
      541 tagcgacgct ggccatgaaa ttgctaatca tacttgggat catcccaatc tgactaatct
      601 ttcagtcagc gagattcaac atcaagttaa tatgacgaac caggctattg aaaaagcttg
      661 tggcaagaaa cctcgctatt tacgccctcc atacggagct acaaatgcaa ctgttcagca
      721 atcctcagga ttaacacaaa tgctttggac ggttgataca agagattggg aaaatcatag
      781 cactgatggc attatgacta atgtcaaaaa ccaattgcaa ccgggaggag ttgttttaat
      841 gcatgatatt catcaaacaa ctatcaatgc tttgccggca gttatggaat atctaaaagc
      901 agaaggatac gaatgtgtga ctgtatcaga actctatgcg catcagtaag taatatcgtt
      961 atgtttcttt agaattgata tactaaacaa tccaattact ttgaagtaat tggattgttg
     1021 tttttagtag cccttgatta agttttgttt tttaacaact agttgatgaa atgaagttgt
     1081 tgatttttga gggagatttc agagacttta gcaaattttt tcaatttttt gaggtcttca
     1141 gggtggctaa caaaggtaat aacgatacca gattttccca tacggccggt tcgtccagct
     1201 ctgtgagtat aattttcctt atcacgagca acatcaaaat gaatgacata gtcaagatta
     1261 tcaatatcaa tgccccgagc aacaagatcc gtagctaaca ataaggagag ttggtgggat
     1321 ttgaattttt cgagaatggt tttacggaat ttgacattga tgtcactcgc taaggaaacc
     1381 gcagcagctc cattatattg gagacgttct tccgtagcac ccaaatcaga taaactgttg
     1441 aaaaagacta aagctctaaa atgaggaata ttagcaaatt ttcgtaataa atcggtacgt
     1501 tcgcgcttgt caaccatgag gtaaaaatgc tgaatagcat cgttggtttg ctctgaaaga
     1561 tcgatgcaga atgtatttgg agctaaggaa gtttgatcca ccttattagt agcactcatg
     1621 tataccattt gatgatctcg aggaacatag tggctaattt tttgaacaaa atcatattgg
     1681 gaatcaccta atagttcgtc atattcatct aaaacaatgg tgttgacaga catcatcttg
     1741 atttttttca atttaatcag ttcaaaaatg cgtccagggg tcccaatcag gatttcagga
     1801 ccttttttga ggcgttcgat ttgacgtttt tgactggttc cagaaataaa gagttgtgcc
     1861 gttaagccta gtggctgtgc ccaatccttt gtgacttcaa aaatttggcc agctaattcg
     1921 gtattagggg ctaaaatgag tagttgttgg gactttttag gcgtcagtgc caatagacta
     1981 ggaaagacat aagctaaggt cttgcccgtc ccagtagggc taataccaag gaaattttta
     2041 ccatcaacaa taggttgaaa ggcctgttct tggattggcg ttaaatgggt aaatgcaact
     2101 tggtcaagtt tttcttgcca ttgaggggga aatttagtga tcatgcgtct tccttttact
     2161 attgtttcta tattcattat agcattatag tctcaatttt ggtaaaatag tgagaaagac
     2221 tttttagatt tggagtttaa aatgcttaaa aaaccgatta ttattggtgt aactggtggt
     2281 tcaggtggcg gtaaaacaag tgtgtcacgc gctattttgg acagttttcc aaatgctcgt
     2341 attgcgatga ttcaacacga ttcttactat aaagatcagt ctcacatgag ttttgaagaa
     2401 agggtcaaaa ccaattatga ccatcctcta gcttttgata cagattttat gattcagcaa
     2461 ctaaaagaat tattagctgg tagacctgta gatattccta tctatgatta caaaaagcat
     2521 acccgcagta atacgacctt tcgtcaggat cctcaggatg ttattattgt tgaaggtatt
     2581 cttgtcctag aagatgagcg tttgcgagat ttgatggata tcaagctctt tgtagacaca
     2641 gatgacgata ttcgtattat tcgtcgtatt aagcgtgaca tgatggaacg tggcagaagc
     2701 ttagaaagta ttattgatca gtacacaagc gtggtaaagc ctatgtatca tcagtttatt
     2761 gagccaagta aacgctatgc tgatattgtt attccagagg gagttagtaa tgtcgttgct
     2821 attgatgtta ttaatagtaa aattgcgagt attcttggag aagtatagtg gaagtggctg
     2881 agtagattat ttaaatctgc tcttttattg ttgtgctctt catagagaaa gggatcataa
     2941 aatagaaatg ttattaattt ggcttggatt actatttgtt tggaatgaac tcgtgtttgt
     3001 catatatgcc tttgataaaa gaaaagccat aaaaaagaaa cggcgtatta gtgaaagaaa
     3061 cttattagtg ataacagttt tgtttggagg ttttggagca ttacttgcag ctaaaaagta
     3121 ccaccataaa accagaaagt ggtactttgt catcacttgg tatactagta ttttgttgac
     3181 attactagta acttatatct ttatgaagtg agtcacttaa aagaccctta atcagtatta
     3241 tttagtcaag gctcttttat agtttaaaaa agcacatcat cgtcaagggt gttgattttt
     3301 tgaaaaagat caccaaagat taaaaggatt aatgttccta gtaaaactgt catgccagga
     3361 atgcttgtta gaggagagtt ccagatgaaa tgtagcacca gaggacttaa taaccagaaa
     3421 atttgttgct gttttgaaaa aaggttagaa ccctttaata ataagtatgc tccaatggta
     3481 aaaatagctg tatgtaccca atgtgaagtc acagcagtgc taatacgttc aatagcagtc
     3541 ggtacgagac tatctataga ttttgcttga atcaaataac ggatatcttc tgctagttga
     3601 aagcccatgc cagaaagtaa agcaacaact aatttttctt taagagtaag tctaggaaat
     3661 aaagtaatta ctaaaatagc cgaagaagcc ttaatgggct cttcgacaat aggggcagtt
     3721 aaagcatctc cccaaagctt taaagcgtct ttagaaggga tgatgtatga ccaaaattgt
     3781 cctacgagat ggttcccaca agaagctaaa aaacctgaaa tataaagtcc gctaagacag
     3841 ctaacaataa taacattcag gttcatatga aattttttac ttaaataaaa aatagctgct
     3901 gcaaatggaa ttaagtaaat gcctaataga ctaatggtta gtattagagc aaaagcttct
     3961 ttaaaggaaa ggttgttagc tgtcagtcct aacaattcaa gttcaatacc atttaaaact
     4021 aaagcaataa aaagaccgta tcgtaaccat ttctgttgag tgatttgttt cacgaaaacc
     4081 tcctaatatt aagatgatat taataatatt gaaaattata tcataaacaa cctcaaaaac
     4141 cattttaaaa attgactgaa gcgaaagata gtcgtaaaaa atactcgaat agacccagat
     4201 atgctataat aaaaggtatg aataagtcta aaaaaattga gcaataccaa ctaatgatag
     4261 cacaggcaaa agagctgttt gcgaatgaat caaatgcttt ggctaatttg tcaaatgcca
     4321 gcgccctgtt aaacatgaca ctacccaatt ctgtttttgc gggattttac ctttttgatg
     4381 ggcaggaatt aattcttggc ccttttcaag gtagggtttc ttgcgtccat atcaagcttg
     4441 gtaaaggtgt ttgtggcgaa tcagctcagt ctcgtcgaac cattattata aatgatgtga
     4501 agcagcatgc caactatatt tcctgtgatg cggcagccat gagtgaaatt gttgtgccta
     4561 tggtgaaaga aggccatttg attggggtgc tggacttgga ttctagcctt gtagctgatt
     4621 atgatgaggt tgatcaagaa tacttagagg cctttgtgga cctatttctt gaaaagacca
     4681 cgtttacctt taacatgttt ggagttaaaa actgatgtat caagctcttt atcggaaata
     4741 ccggagccaa acgtttgacg aaatggtggg acaatcggtt atttccacaa ctttaaagca
     4801 ggcagttgaa tctggcaaga ttagccatgc ttatcttttt tcaggtccta gagggactgg
     4861 gaaaacaagt gcggcaaaga tttttgcaaa ggccatgaat tgtcctaacc aagtcgatgg
     4921 tgaaccctgt aatcaatgcg atatttgccg agatatcacg aatggaagct tggaagatgt
     4981 gattgaaatt gatgctgcct cgaataatgg tgttgatgaa attcgtgaca ttcgagacaa
     5041 atcaacctat gcgccaagtc gtgcgactta taaggtttat attattgatg aggttcacat
     5101 gttatcaaca ggggctttta atgcgctttt gaaaactttg gaagaaccga cagaaaatgt
     5161 tgtctttatc ttggcaacaa cggaattgca taaaattcca gccactattt tatctcgtgt
     5221 gcaacgcttt gaattcaaag ctattaagca aaaagctatt cgagagcatt tagcctgggt
     5281 tttggacaaa gaaggtattg cctatgaggt ggatgcttta aatctcattg caaggcgagc
     5341 agaaggaggc atgcgtgatg ctttatctat tttagatcag gctttgagct tgtcaccaga
     5401 taatcaggtc accattgcaa ttgccgaaga aattacaggt tctatttcca tgcttgctct
     5461 gggtgactat gttcgatatg tctcccaaga acaggctacg caagctctgg cagccttaga
     5521 gaccatttat gatagtggga agagcatgag ccgctttgcg acagatttat tgacctatct
     5581 gcgtgattta ttggtggtta aagctggcgg tgacaatcag cgtcagtcag ctgtttttga
     5641 taccaatttg tctctctcga tagatcgtat attccaaatg ataacagttg ttactagtca
     5701 tctccctgaa atcaaaaagg gaacccatcc tcggatttat gccgaaatga tgactattca
     5761 gctagctaaa gaagaaggag tagcgttacc atcaggtttg tcatcagatg tgacgactga
     5821 agttgaggga cttaaacgtg aacttaatca gcttaagcaa gccttggcac aattgcaagc
     5881 cagtcccagt ccctcacatg ctcctgtaaa accagcacag gctaaaccga aactaaccac
     5941 ttacaagatt gatcgggtga aaatactgaa aatcatggaa gaaacggttc aagatagtca
     6001 gcaatcacgc caatacttgg atgccctaaa aacagcttgg aatgaaattt tagacagtat
     6061 ctcagctcaa gatagagcct tactaatggg atcagagcct gttctggcca atagtgagaa
     6121 tgctatttta gcttttgaag ctgcttttaa tgctgagcaa gcaatgaagc gtactgattt
     6181 gaatgcgata ttcggcaaca ttatgagcaa agcagcaggc ttctcaccta atattttggc
     6241 agtccctaaa gctgattttc atcacattcg ttctgagttt gccttacaat tgaaaactca
     6301 aaaggcttct gaccctacag aagagacgat agaagaaacc cttgagattc cagaaggctt
     6361 tgattttcta gttgataaaa ttaatgccgt tgaggattaa acattattag ctgttaatga
     6421 accagtaacg ctataaaggt aagcctattg ggcttacctt tagcgttttt ttatctaata
     6481 taccaagata gtttttctag ccaatcctct aatagtagaa gtgaaaccaa aaaagattga
     6541 tgctttcttg ggtatccaag tcagcatcaa tctgcacgcc aaacgcatga agaaactttt
     6601 gttttcagaa aactagattc ataatgacaa aatcaatcaa agtagtacac tatagatgag
     6661 gtgactacga tgattgattt tattatttct attgatgatt gcgcagttga attggatagt
     6721 cgtcaatctt ggaaaatt
//
LOCUS       DATXQK010000064         6718 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.064, whole genome shotgun sequence.
ACCESSION   DATXQK010000064 DATXQK010000000
VERSION     DATXQK010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6718
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.064"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            15..788
                     /gene="cfa"
                     /locus_tag="VC394_001555"
     CDS             15..788
                     /gene="cfa"
                     /locus_tag="VC394_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_149017463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAMP factor pore-forming toxin Cfa"
                     /protein_id="HEP5995010.1"
                     /translation="MKPKHLLCLSTVVAGLALFSTMTHSVLADDASNPDTILMNNNQA
                     NLQRDALVQKLDEGHQQLEAIKHEAKGTDIEATVNKAIDAVDHMKSSIRFNTETIYDF
                     SSIGARVEALSDAIKAIVFSTTQLTHKVEKAHTDMGFAITKLVIRIIDPFASVDAIKA
                     QVQEIKALEEKVINYPDLQPTDRATIYTKAKLNKAIWNTRLERDKKVLGIKSFDVYNR
                     LNKAITHAVGVQLNPTTTVQQVDDEVIAVQNALETALKS"
     gene            complement(1158..2393)
                     /locus_tag="VC394_001556"
     CDS             complement(1158..2393)
                     /locus_tag="VC394_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEP5995011.1"
                     /translation="MTQDPIANLKLARKGPIVSIIVYLLLSAAKLLAGYLLNASSLIA
                     DGFNNLSDIVGNVALLIGLHLASQPADANHKFGHWKIEDLSSLVTSFIMFLVGFQVLI
                     HTIKRIFSGQQVDIDPLGAIVGIVSAFVMLGVYVFNKRLSKRVKSSALVAASKDNLAD
                     AVTSIGTSIAIIAASLHLPVIDHIAAMIITFFILKTAFDIFMESSFSLSDGFDSRHLK
                     KYEKAILEIPKIVAVKSQRARTYGSNVYLDIVLEMNPDLSVYESHSITEKVEQLLSDQ
                     FSIYDIDIHVEPAMILEEEIFDNVAKKLYRYEKLILSKVPDYDHYIAKSFQLVDANGQ
                     TVNYEQFLNQEIYYPSNFNHFQIESISQKTMLVTYQLNGNQHTSIWRRHESWSLLFHQ
                     ITPIAKRQLHHTHYRIVKM"
     gene            complement(2513..3835)
                     /locus_tag="VC394_001557"
     CDS             complement(2513..3835)
                     /locus_tag="VC394_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEP5995012.1"
                     /translation="MIALVKLIDNLVWGPPLLILLVGTGIYLTSRLGLIQILKLPRAF
                     KLIFSDDEGHGDISSFAALATALAATVGTGNIVGVATAIKSGGPGALFWMWVAAFFGM
                     ATKYAEGVLAIKYRTKDANGHISGGPMYYIVNGMGTKWKPLAILFAGSGILVALFGIG
                     TFAQVNSITSSLGHSFGLSPQMVSIVLAIFVAAIIFGGIHSISKVAEKVVPFMAIFYI
                     LSSLAVIFSHYQQLLPVIRLVFQSAFTPTAAIGGFAGSLMKDAIQKGIARGVFSNESG
                     LGSAPIAAAAAKTNESVEQGLISMTGTFIDTIIICTLTGLSILVTGQWTGQLEGAPLT
                     QSAFATVFGNLGTFGLTFSLVLFAFTTILGWSYYGERCFEFLFGITHLTYFRIVFILM
                     VGLGGFLKLELIWVLADIVNGLMALPNLIALLALSPVVILETKHYFIK"
     regulatory      complement(3963..4049)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            4364..6682
                     /gene="pcrA"
                     /locus_tag="VC394_001558"
     CDS             4364..6682
                     /gene="pcrA"
                     /locus_tag="VC394_001558"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEP5995013.1"
                     /translation="MNPLLNGMNDRQAQAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKFVNPWNILAITFTNKAAREMKERALSLNPATKDTLIATFHSMCVRILRREADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNIDPKKWNERSILGTISNAKNDLLDEKGYEAQA
                     ADMYSQIVARCYKAYQEELRRSEALDFDDLIMMTLRLFDANPDVLAYYQQRYQYIHVD
                     EYQDTNHAQYQLIKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPDAKVVL
                     LEENYRSTKKILQAANDVINNNRNRRPKKLWTQNADGEQLVYYRANDERDEAVFVAST
                     ISNMSQELGKNFKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDLIA
                     YLTIVANPADNISFERIVNEPKRGVGPGTLDKLRQFAYESDQSLLEAASNLLMSPLKG
                     KAAQAIMDLANILGQLRQDLDQMSITDLAEALLEKTGYLDSLRLQNTLESQARIENIE
                     EFLSVTKNFDESSASQEEDETGVDRLGRFLNDLALIADTDDSQAEAAEVTLMTLHAAK
                     GLEFPVVFLIGMEEGVFPLSRASEDPDELEEERRLAYVGITRAEEVLFMTNANTRTLF
                     GKSSYNRPTRFLKEISEELLSYKGLARPAQSSFGVRFSTETHTQFGQGMSLSEALQAR
                     KAQAQVRQSAQPMQAHTIPSASTSSVLPFGSNSSVEEVTWQIGDIAHHKKWGDGTVLE
                     VSGSGKTMELKIKFPEVGLKKLLASVAPIEKK"
BASE COUNT         2247 a         1267 c         1329 g         1875 t
ORIGIN      
        1 tcaggaggaa taatatgaaa cccaaacacc ttctctgtct ttcaacagtt gttgctggat
       61 tagctttatt ttcaactatg acgcactcag ttttagctga tgatgcttca aatcccgata
      121 ctattttaat gaataacaac caagcgaatc ttcaaagaga cgctctagtg caaaagcttg
      181 atgagggaca tcaacaatta gaagctatta aacatgaagc taaaggtact gatattgagg
      241 ctactgttaa caaagctatc gatgctgttg atcacatgaa gagttctata cgcttcaata
      301 ctgaaacgat ctatgatttt agttcaattg gggcaagagt agaagcatta tcagatgcta
      361 tcaaagcaat cgtattttcc acaacccaat taactcacaa agtagaaaaa gcgcatactg
      421 atatggggtt tgctattact aaattagtca ttcgtattat agatccattt gcatctgttg
      481 acgctatcaa agctcaagta caagaaatta aagcacttga ggaaaaagtt atcaattatc
      541 ctgatttaca gccaacagac cgtgctacta tctatactaa agctaaacta aataaagcta
      601 tttggaacac tcgtttagaa agagataaaa aggttttagg gatcaaatca tttgacgttt
      661 ataatagact taacaaagct atcacacatg cggttggtgt ccaactaaat ccgacaacca
      721 ctgttcaaca agttgacgat gaagttattg ctgtgcaaaa tgctttggaa acagctctta
      781 agtcatagag tgtataagta atgtgttgta taggtagatg attatattag gatatagaat
      841 aggtaaagat tttaaatatc ttaccattgt agagtttgag gtgatgttct cataaacaat
      901 gagtcacctg tagccctttt tgcctttaac atgatgtacc aatttaaggt attgttcaac
      961 cgtcactatt gaatcttgtt taacctccta aactaataag attcaatatg aaaacgtgta
     1021 ttcttaaaaa aatccctatg atctgtttaa aaacaaattg tagggatttt tttaactatt
     1081 taacttatta ttttgggact aggaatcgct ttaggaatat catgaagtat agttatgtta
     1141 taagataagt aatgttttta catttttaca atacgatagt gtgtgtgatg taattgtctc
     1201 ttagcgatag gggtaatttg gtggaagagt aaggaccaag attcatgacg cctccaaata
     1261 ctggtatgtt gattgccatt taattggtaa gttaccaaca tcgttttttg actaatggat
     1321 tcaatctgaa aatggttgaa gttacttgga taataaattt cttggttcaa aaattgttca
     1381 tagttaactg tttggccatt cgcatcaacc agttggaaag acttagcaat gtagtggtca
     1441 tagtcaggaa ccttactcaa aattaatttt tcgtagcggt agagcttttt ggcgacatta
     1501 tcaaaaatct cttcttcgag aatcatggca ggttcaacgt gaatatcaat gtcataaata
     1561 gaaaattggt cactcaataa ctgctccact ttctctgtaa tagagtgact ttcatagact
     1621 gaaagatcag gattcatttc aagtacaata tcaagataga cattgctacc ataggtccta
     1681 gctcgttgag acttaacggc gacaatttta gggatttcta aaatggcttt ttcgtatttt
     1741 ttcaaatgac ggctatcaaa tccatcagat aaactaaacg aactttccat aaagatatca
     1801 aaagctgttt taagaataaa gaacgtaatg atcatagcag ctatatgatc gataactggt
     1861 aaatgcaaag aagctgctat aatagcaatt gatgttccga tagaagtaac agcatcagct
     1921 agattatcct tagaagcagc gactaaggca ctggatttta cacgtttgga aagacgtttg
     1981 ttaaagacat agacccctaa cataacaaaa gctgaaacga taccgacaat agccccaaga
     2041 gggtcaatat caacttgctg accactaaag atacgtttaa ttgtgtgaat cagtacttgg
     2101 aaacctacaa gaaacataat aaaagaagtg acaaggctgg ataaatcttc aattttccaa
     2161 tgaccaaatt tatgattggc atcggctggt tggctagcta agtgaagacc aataagcagg
     2221 gctacatttc ccacaatatc cgataaattg ttaaatccat cagcaataag ggaacttgca
     2281 tttaacaaat aaccagctaa taatttggca gcactaagca acaagtagac aataatacta
     2341 acgattgggc cctttctggc tagttttaaa tttgcaatcg gatcctgtgt cataataact
     2401 cctttgattt caactttata ttataccata aaaaggggct taagaaaaaa gcctagcttc
     2461 ttgaacttgg cagaagaaga ctaggtataa ctgggtagta ctaacgtcat gtttacttaa
     2521 taaagtaatg cttggtttct aaaataacaa ctggcgagag agctaggagg gcaatcaggt
     2581 taggaagtgc cattaaacca ttgacaatat cagccaaaac ccaaatcagt tctaacttaa
     2641 ggaagcctcc taggcctacc attaagataa agacaatacg gaagtaggtg agatgtgtaa
     2701 tgccaaagag gaattcaaag cagcgttctc cataataact ccatcctaaa atcgttgtaa
     2761 aggcgaagag aactagggaa aaggttaggc caaaagtccc taaatttcca aacacagtgg
     2821 caaaggcaga ttgggtcaga ggagcccctt cgagctgccc agtccattgg cctgttacta
     2881 aaatagatag gccggttagg gtacagataa taatcgtatc aataaaagtt cctgtcatgg
     2941 agatcaggcc ctgctccacg gattcattgg ttttagctgc tgctgcagca ataggtgcag
     3001 atcctagtcc agactcattg gagaagacac cgcgtgcaat tcctttttga atggcatcct
     3061 tcatgagact tccagcaaag cctccaatag cagctgtcgg agtgaaggct gattgaaaga
     3121 ctaaacgaat aacaggaagt agctgctggt agtgactaaa aataactgct aaactagata
     3181 gaatataaaa aatagccata aaaggaacga ctttttcagc cactttggaa atggaatgaa
     3241 tgccaccaaa aataatggca gccacaaaga tagctaaaac aatactgacc atttgtgggg
     3301 ataagccaaa gctatgacct aaagacgagg tgatcgagtt cacttgagca aaggttccta
     3361 ttccaaatag ggcaactagg atccctgaac ccgcaaaaag aatggctaag ggtttccatt
     3421 ttgtccccat gccattaaca atatagtaca taggaccacc agaaatatga ccgttggcat
     3481 cctttgtacg gtatttaatc gctagtaccc cttcagcgta cttagtggcc attccaaaaa
     3541 aagcggcaac ccacatccaa aagagcgctc caggaccacc agacttgata gcagtggcaa
     3601 ccccaacaat gttaccagtt ccgacagtag cggcaagggc agttgcaaga gcagcaaagg
     3661 atgaaatatc tccatgtcct tcgtcatctg aaaaaatgag tttaaaggct cttggtagtt
     3721 ttaagatttg aattaatcct aaacgactgg taaggtaaat ccccgtccca accaataaaa
     3781 ttaagagggg cggtccccaa acaaggttat caattaattt aacgagtgct atcatctttc
     3841 tctcctaaaa gtcatcgaag acgccaaaag aaaaaagcta tctctttctt tgagacaact
     3901 tctgaagcaa tgataaaaca gcaaacacaa aaaagactaa gactgttctt aatggtgttt
     3961 gtattttacc ttctgtcctt ttgcctgaga gattgagcag tatgccttgc cccttcggcg
     4021 cctaataagg tctctccaga agtccgtcca tcaaacagtc ccgttcaaaa actgaacacc
     4081 tgagagtata actccttcgg cgaaaaaaat tcttctcctg catgacatca ttcggtttat
     4141 taaattagga ctatcatacc tcaaaaaacg aatgaagtca accaaaatag caaaaaaata
     4201 aggaatgctt taagaaatcg ctattatttt taaatatctg atcctaaact agaaaaaagg
     4261 aatctcttta cttttggggt cttctttgct atttttcttg gaaacatgat ataattttaa
     4321 ggttgataaa agagatgtca ctgaacgaga ttaaggagaa agcatgaatc ctttattaaa
     4381 tggaatgaat gatcggcagg cacaagctgt tcaaacaact gaaggcccct tattgattat
     4441 ggcgggggct ggttctggta aaacaagggt actgacccat cgtattgcct atttaattga
     4501 tgaaaaattt gtcaatcctt ggaatatttt agctattacc tttactaata aggctgcgcg
     4561 tgaaatgaaa gaaagagcct tgtccttaaa tccagctact aaagatacct taattgctac
     4621 ctttcactcc atgtgtgtac ggattctgcg tcgtgaggct gatcacattg gttacaaccg
     4681 aaacttcaca atcgtggatc caggtgagca acgaacgttg atgaagcgca ttttaaaaca
     4741 attaaatata gaccctaaaa agtggaatga acgttctatt ttaggaacta tttcaaacgc
     4801 taaaaatgat ttgcttgatg aaaagggata cgaggcacag gctgctgata tgtatagtca
     4861 aatcgtagcg aggtgttaca aagcttatca ggaagaatta cgacgtagcg aagcacttga
     4921 ctttgatgat ttaatcatga tgacgctacg cctgtttgac gctaatcctg acgtgttagc
     4981 ttattaccag cagcgttacc aatacattca tgtggatgag taccaagata ccaaccatgc
     5041 tcaatatcag ctaattaaat tgctagcttc tcgctttaaa aatatttgcg ttgttggaga
     5101 cgctgatcag tctatctatg gttggcgggg agctgacatg caaaatattc ttgattttga
     5161 aaaagattac ccagatgcta aggtagtgct tttagaagaa aactatcgct ctaccaagaa
     5221 aattctgcaa gcagctaacg acgtgattaa caataaccgt aatcgccgtc ctaagaaact
     5281 ttggacacaa aatgcggatg gtgaacaact ggtttattac cgggcaaatg atgagcgaga
     5341 tgaggcagta tttgtggcct caaccattag caatatgagt caagagcttg gaaaaaactt
     5401 caaagatttc gctgttttat accgtaccaa tgcccaatct cggaccattg aagaagcact
     5461 cttaaaatcc aatattcctt acaccatggt tggaggcacc aagttttaca gccgtaaaga
     5521 aattcgggat ttgattgcct atttaaccat tgttgctaac cctgctgata atatttcttt
     5581 tgagcggatt gtgaatgagc ctaaacgagg tgttggtcca ggaacgcttg ataaactgcg
     5641 tcagtttgcc tatgagagtg accagtcttt attagaagca gcttcaaatc tattgatgtc
     5701 ccccttgaaa ggtaaagcag cacaagctat catggacttg gccaatattc tcggtcagct
     5761 acgtcaagac ctagatcaga tgagtatcac cgacttagca gaagctttgt tagaaaaaac
     5821 aggttattta gatagcttac gcctgcaaaa tacattggaa agccaagccc gtatcgaaaa
     5881 tatcgaagaa ttcttatctg ttaccaaaaa ttttgacgag tcaagtgcct ctcaagaaga
     5941 agacgaaacc ggtgttgatc gcttgggacg ttttttaaac gatttagcac tgattgctga
     6001 tacagatgat tcccaagcag aagctgctga agtaacctta atgaccctac atgctgccaa
     6061 aggcttggag ttccctgttg ttttcttaat tggtatggaa gaaggtgtct tccccttatc
     6121 acgtgcctct gaagatccag atgaattgga agaagaaaga cgtttagctt atgtcggaat
     6181 cacaagagct gaagaagttc tctttatgac caatgccaac accagaaccc tctttggtaa
     6241 aagtagttat aaccgtccaa cacgcttctt gaaagaaatt tcagaggaac tactctctta
     6301 taaaggattg gcaagaccag cacaatcttc atttggtgtg cgtttttcta ctgaaacaca
     6361 cacacagttt ggacaaggga tgagcctatc agaagcctta caagctcgaa aggcccaggc
     6421 tcaagtaaga caatcagcac agccaatgca ggcacatacg ataccatcag cttcaactag
     6481 ctcagtttta ccgtttggtt ctaatagctc tgtagaagag gtcacttggc agattggcga
     6541 tatcgctcac cataaaaaat ggggagacgg gactgtgctt gaggtttcag gctccggaaa
     6601 aacgatggaa ttgaaaatca aatttccaga agttggtcta aagaaattac tagcaagtgt
     6661 tgctcctatt gagaaaaaat aagaaaaaaa tgagcttcct actatataag tgagtagg
//
LOCUS       DATXQK010000065         6707 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.065, whole genome shotgun sequence.
ACCESSION   DATXQK010000065 DATXQK010000000
VERSION     DATXQK010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6707
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.065"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..2534)
                     /locus_tag="VC394_001559"
     CDS             complement(<1..2534)
                     /locus_tag="VC394_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEP5995014.1"
                     /translation="MKNNNKWIIAGLASFLFPLSIIFIILLSMGIYYNSDKTILASDA
                     FHQYVIFAQNFRNIMHGSDSFFYTFTSGLGINFYALMCYYLGSFFSPLLFFFNLTSMP
                     DAIYLFTLIKFGLIGLAACYSFHRLYPKINAFLMISISVFYSLMSFLTSQMELNSWLD
                     VFILLPLVVLGLNKLITENKTRTYYLSISLLFIQNYYFGYMIALFCILYALVCLLSLN
                     DFNKMFIAFVRFTAVSICAALTSALVILPTYLDLSTYGENLSPVKQLVTNNAWFLDIP
                     AKLSIGVYDTTKFNALPMIYVGLFPLMLSVIYFTLESIPLKIKLANACLLTFIIISFY
                     LQPLDLFWQGMHSPNMFLHRYAWSFSIVILLLACETLSRLKEVTQIKAGFAFIFLIIL
                     TSLPYSFSQQYNFLPLTLFLLSVFLLLGYTISLFSFRNSQIPPTFISAFILIFSLLES
                     GLNTYYQLQGINKEWGFPSRQIYNSQLKDINNLVNSVSKNSQPFFRMERLLPQTGNDS
                     MKFNYYGISQFSSVRNRLSSSLLDRLGFQSKGTNLNLRYQNNTIIMDSLLGIKYNLSE
                     GPLNKFGFTKLKTSGNTTLYQNHYSSPLAILTRNVYKDVNLNVNTLDNQTKLLNQLSG
                     KSLTYFNLQPAQLISGANQFNGQISAQASDYQNSVTLNYQINIPKHSQLYVSIPNIIF
                     SNPDAKEVRIQTDNHNFIYTTDNAYSFFDLGYFADAKVATFSFVFPKNKQISFKEPHF
                     YSLSIESYLEAMNSIKQKNVHTYAKSNTVITDYNSKTKGSLIFTLPYDKGWSAQKDGK
                     NLPVKKAQGGFLSVTIPKGKGRVILTFIPNGFKLGLSLSCVGIIAY"
     gene            complement(2617..4236)
                     /locus_tag="VC394_001560"
     CDS             complement(2617..4236)
                     /locus_tag="VC394_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEP5995015.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTAGNTYGLIGANGAGKSTFL
                     KILAGDIEPSTGHISLGPDERLSVLRQNHFDYEEERAIDVVIMGNEQLYNIMKEKDAI
                     YMKADFSEEDGVRAAELEGIFAELGGWEAESEASQLLQNLNIPEDLHYQNMSELANGD
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSISWLEDFLIDFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLFVGNYDFWKQSSELAARLQADRNAKAEEKIKELQEFVARFSANA
                     SKSKQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREMGNDFLTVENLSVTIDGEKI
                     IDNISFILRPGDKAAIIGQNDIQTTALMRALADDIDYEGTIKWGVTTSRSYLPKDNSK
                     DFATEESILEWLRQFASKGEDDDTFLRGFLGRMLFSGDEVKKSVIVLSGGEKVRVMLS
                     KLMLLKSNVLILDDPTNHLDLESISSLNDGIKDFKESVIFASHDHEFIQTIANHIVVI
                     SKNGVIDRIDETYDEFLDNPEVQARVAELWK"
     gene            complement(4315..5187)
                     /locus_tag="VC394_001561"
     CDS             complement(4315..5187)
                     /locus_tag="VC394_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEP5995016.1"
                     /translation="MKKRGIDLLLVTIGSFITAIGFNTMFVDNHIASGGMVGIAVVIK
                     ALFGISPSLFLMASNIPLLLMCYFFLGKQNFIKTLYGSWIYPIAIRSTNSLPTLTHNQ
                     LLAAIFGGIICGIGLGMVFWGNSSTGGTGILTQILHKYSPLSLGVAMTIVDGISVLMG
                     FIALSADDVMYSTIGLFVIGYVISVMENGFDSSKNVMIISKDYQAIREYITTVMDRGV
                     TKLPIRGGYTTSDKIMLMAIVSSHELPTLQEKILEIDDTAFIVVMPAAQVMGRGFSLT
                     KQYKREDKDVLLPM"
     misc_binding    5310..5551
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5606..6628
                     /gene="trpS"
                     /locus_tag="VC394_001562"
     CDS             5606..6628
                     /gene="trpS"
                     /locus_tag="VC394_001562"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEP5995017.1"
                     /translation="MTKPIILTGDRPTGKLHLGHYVGSLKNRVFLQNENKYKMFVFLA
                     DQQALTDHAKESELIQESIGNVALDYLSVGLDPKQSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEIAQKGFGESIPSGFLVYPVSQAADITAFKANLVPVGNDQKPMIE
                     QTREIVRSFNHTYHTDCLVEPEGIYPENEKAGRLPGLDGNAKMSKSLGNGIYLSDDAD
                     TVRKKVMSMYTDPNHIKIEDPGQIEGNMVFHYLDIFARKEDQADIEAMKEHYQRGGLG
                     DVKTKRYLLDILERELAPIRERRLEYAKDMGEVFRMLQEGSQKARTVAAKTLSEVKSA
                     MGINYF"
BASE COUNT         2330 a         1080 c         1221 g         2076 t
ORIGIN      
        1 atataagcga taattcctac gcaagataga gataacccta atttaaaacc attaggaata
       61 aaggtaagga taacacgtcc ctttccttta ggaatagtaa ctgatagaaa tcctccttgt
      121 gcttttttga ctggaagatt tttcccatct ttttgtgctg accaaccttt atcgtaagga
      181 agtgtaaaaa taagagaacc tttcgttttt gaattataat cagtgattac cgtattactt
      241 ttagcgtaag tatgaacatt tttttgttta atactattca ttgcttcaag gtaagattca
      301 atagacaaac tataaaaatg aggttcctta aaactaattt gtttattttt tggaaaaaca
      361 aacgaaaatg tagcaacttt ggcatcggcg aaatatccta aatcaaaaaa agagtaagcg
      421 ttatctgtag tatatatgaa attatgatta tctgtctgaa tacgcacctc tttagcatca
      481 ggatttgaaa atataatatt gggtatgcta acatagagtt gactatgttt agggatgtta
      541 atttgataat taagggtaac ggagttttga taatcagaag cttgtgcaga tatttgtccg
      601 ttaaattgat tagcaccaga aataagttga gctggctgta agttaaaata ggttaaagat
      661 ttcccactta gttggttaag taatttggtt tggttatcaa gggtattgac atttagattg
      721 acatctttgt aaacattacg tgttaatata gctaaagggc tactatagtg attttgataa
      781 agagtagtat tcccgctagt ttttagtttt gtaaatccaa atttatttag aggtccttcg
      841 ctaagattat attttatacc aagtagactg tccataataa tagtattgtt ttggtatcta
      901 aggtttaaat tcgtgccttt agactgaaat cccaatcgat ccaataaaga actagatagt
      961 ctatttctta cagaggaaaa ttgtgaaatg ccgtaataat taaatttcat gctatcgttc
     1021 cctgtttggg gaagtagtct ttccattcta aaaaaaggtt gactattttt tgacacagag
     1081 ttgacaaggt tgttaatatc ctttaattga ctattatata tctgtcgtga tgggaatccc
     1141 cactccttat taattccttg aagctggtag taggtgttta accctgattc aagaaggcta
     1201 aagataagta tgaaagcaga aataaaagta ggtgggattt gagaatttct aaacgaaaat
     1261 agtgaaatag tataacctaa taataaaaaa acactaagta aaaaaagagt taaaggtaga
     1321 aaattatatt gttgagaaaa gctataagga agagatgtca gtataatgag gaaaataaaa
     1381 gcaaaacctg cttttatttg agtcacttct tttagtcgag agagagtctc acatgcgagt
     1441 aataggataa ctatggaaaa agaccaagcg tagcgatgca aaaacatatt tggtgagtgc
     1501 atcccctgcc aaaaaagatc aagtggctgt aggtaaaaac ttattataat aaaagttaac
     1561 aagcaggcat tggctaattt tatttttaaa gggatacttt ctaaagtaaa ataaataaca
     1621 ctaagcatta ggggaaataa tcctacgtaa atcataggca gagcattaaa cttggtagta
     1681 tcgtacactc ctattgagag cttagcaggt atatccaaaa accaagcatt gttcgtaact
     1741 aactgtttta ccggggatag attctctcca taagttgaca aatctagata ggtaggaagt
     1801 attaccagag cacttgttaa agcagcacat attgacacag ctgtaaacct aacaaaagcg
     1861 ataaacattt tgttaaaatc attgagactt aaaagacaaa ctaaggcgta aagaatacaa
     1921 aaaagagcaa tcatgtagcc aaagtagtaa ttttgaatga ataataatga tatcgaaaga
     1981 taataagttc tggttttatt ttctgtgata agtttattta atccaagtac aacaagtgga
     2041 agaagaatga aaacatctaa ccaagaattt agttccattt gacttgtcaa gaagctcatt
     2101 aagctataaa aaactgagat ggaaatcatc aagaaagcat tgatttttgg atataatcta
     2161 tgaaaagaat agcatgcagc taatcctatt aacccaaatt ttatcaaggt aaacaaatag
     2221 atagcatctg gcatagaggt taaattaaaa aagaaaagta atggagaaaa gaaactgcca
     2281 agataataac acattaaagc ataaaaattt atccctagtc cgcttgtaaa ggtataaaaa
     2341 aaactatcag aaccgtgcat gatgttacga aagttctgcg caaaaataac atactgatga
     2401 aaagcatcac tagctagaat tgttttatca ctattataat aaatgcccat cgatagaagg
     2461 atgataaata taatactaag agggaacaaa aaactagcaa gtccagcaat tatccattta
     2521 ttattatttt tcataaatta tatggtcttt cgtttacaaa ttttaaaaaa gaggctagga
     2581 agagatcttc ttagccacct catttatcat aaggttttat ttccaaagtt cagcaactcg
     2641 ggcttggact tctggattgt caaggaattc gtcataagtt tcatctatac ggtcgataac
     2701 accatttttt gaaataacaa cgatgtggtt agcaatcgtt tgaataaatt catggtcatg
     2761 actggcaaag ataacagatt ctttgaagtc tttgatccca tcattaagac ttgaaataga
     2821 ttccaaatcc aagtgatttg ttggatcatc aagaataaga acgtttgatt tcaatagcat
     2881 caacttagaa agcatgacac gcactttttc gccaccggaa agaacgataa cagatttttt
     2941 aacttcgtct ccagagaaaa gcatccgtcc taggaaacca cgtaaaaaag tatcgtcatc
     3001 ttcacctttt gaggcaaatt gacgtaacca ttcaagaata gattcttccg tggcaaaatc
     3061 ttttgaattg tctttaggca agtaagaacg acttgttgtc actccccatt taatggttcc
     3121 ttcgtagtca atatcatctg ctaaagcacg cataagagcg gttgtttgaa tatcattttg
     3181 accaataata gcagctttgt cacctggacg taaaatgaaa ctgatattat caatgatttt
     3241 ctccccatcg atcgttacag aaagattttc aacagtgagg aagtcattcc ccatttcacg
     3301 ctctgcttta aaattaataa atgggtattt acggcttgag ggaacaattt cttctaattc
     3361 aatcttatca agcatttttt tacgagaggt tgcttgtttt gatttagatg cgtttgctga
     3421 gaaacgggca acgaattctt gtaattcttt gattttttct tcggctttag cattgcggtc
     3481 agcttgtaaa cgagcggcta gctcagaaga ttgtttccag aaatcgtagt tacccacaaa
     3541 gagtttgatt ttaccaaagt caaggtcggc catgtgagta catactttat tcaagaagtg
     3601 acggtcatgg gaaacaacga tgactgtatt ttcaaaatca atcaagaaat cttctaacca
     3661 tgaaatagat tgaatatcaa gaccattggt tggctcgtca agtaataaaa cgtcaggctt
     3721 accaaagagc gctttagcaa gaaggacttt cactttatct ccattagcca gttcactcat
     3781 attttggtag tgaaggtctt ctggaatatt tagattttgg agaagttgag aagcttcact
     3841 ttctgcctcc cagccaccta attcagcaaa gataccttct agctcagcgg cacgaacccc
     3901 atcttcttct gaaaaatctg ctttcatata gatagcgtct ttttctttca tgatattgta
     3961 aagttgctca ttacccatga ttacaacatc aatcgcacgt tcttcttcat aatcaaaatg
     4021 attttggcga agaacagata agcgttcgtc aggtcctaaa gagatatgac cagtacttgg
     4081 ttcaatatct cctgcaagga ttttaagaaa agttgatttt ccggcaccat tcgcaccgat
     4141 taagccgtaa gtattgccgg ctgtgaattt gatattaaca tcatcaaata gttttcgatc
     4201 actaaagcgt agtgatacat cagaaactgt aagcaatttg tcacctcatt ttatttatct
     4261 aagcttcatt gtacctaaaa tagtcccctt tttcaatcag aaaagggcta atcactacat
     4321 aggcaataaa acgtctttgt cttcacgttt gtattgttta gtcaaactaa atcctcttcc
     4381 cataacttga gctgctggca tgacaacaat aaaggctgtg tcgtcaattt cgaggatttt
     4441 ttcttggaga gtgggtaatt cgtgacttga tacaatagcc attaacataa ttttatcaga
     4501 agtagtataa ccaccgcgaa taggaagttt agtcactcca cgatccataa cagttgtgat
     4561 atattctcga atagcttgat aatctttgga aataatcata acatttttgg atgagtcaaa
     4621 gccattttcc ataacgctaa tcacataacc aattacgaaa agtccaatcg tagaatacat
     4681 cacgtcatct gcagaaagtg caataaatcc cataaggaca ctaataccat ctacaattgt
     4741 cattgcaact ccaagagata aaggagagta tttatgaagt atttgtgtta gaattccagt
     4801 tcctcctgtc gaagagtttc cccaaaaaac cattcctaaa ccaatgccac agataatacc
     4861 accaaaaata gctgctaaca actgattgtg tgtcagagta ggtaatgaat tggttgaacg
     4921 gatggcaata ggatatatcc aagaaccata aagtgtttta ataaaatttt gttttccaag
     4981 aaagaagtaa cacattaata ataagggaat attactagcc attaaaaaaa gtgacggact
     5041 aatgccaaat agagctttta taacaactgc aataccaacc ataccccctg atgcaatatg
     5101 attgtcgaca aacattgtat tgaaaccaat agctgttatg aatgagccaa ttgtgacaag
     5161 tagtaaatca atccctcgtt ttttcatgct tttaatctcc tttataactg gttaaaaata
     5221 gtagactcat tatctcttaa atgtactgag accgtcaagt catattttca aaaaatctgg
     5281 tgaacttgac aagaagttta aaatttcata gaataaattt gaattagaag agtattcttt
     5341 tcatgttttt agagagttag tggttggtgc aagctaacaa cagaaaaggt gaaatggact
     5401 aatgattatt tgaaaggaaa ccataacttt caggttggca ccccttaccg tgcagcttct
     5461 ttcattgaga agattgagat agtatctcgt tttgataaag cagatgctaa ttgaggtggt
     5521 accgcgtatt acttgtaata acgccctcac gttttaatag cgtggggact ttttgctata
     5581 tcaagagata gttagggaga aaatgatgac aaaacctatt attttaactg gagatagacc
     5641 aactggaaaa ttacatttag gtcattatgt cggaagtctt aaaaatcgtg tctttttaca
     5701 aaatgaaaac aagtataaga tgtttgtttt cttggccgat caacaggcac tgacggatca
     5761 tgctaaagaa tccgaattaa ttcaagaatc tattggaaat gttgccttgg attacctctc
     5821 tgtcggtttg gatccgaagc aatcaacgat ttttattcaa agtcagattc cagagctagc
     5881 tgaattgagc atgtattata tgaatctggt atcattagca cgtttggaaa gaaatcccac
     5941 tgttaaaacc gaaattgctc agaaaggttt tggcgaaagt attccatctg gttttttggt
     6001 ttatccagta tcacaggccg ctgatattac agcatttaaa gcgaatttag tacctgtagg
     6061 taacgatcag aaaccgatga ttgaacaaac acgtgaaatt gtgagaagtt ttaatcatac
     6121 ttaccataca gactgtttag tagaacctga aggtatttat ccagaaaatg aaaaagctgg
     6181 acgcttacct ggtcttgatg gcaatgccaa aatgtctaaa tcattgggaa atggaatcta
     6241 tctttcagat gatgcagata ccgttcgcaa aaaagtgatg agcatgtata ctgatccaaa
     6301 tcatattaaa atagaagatc ctggtcaaat tgaagggaat atggtctttc attatttgga
     6361 tatttttgct agaaaagaag atcaagctga tatcgaagca atgaaagagc attatcaaag
     6421 aggtggttta ggagatgtga aaacgaaacg ctaccttcta gatattttag aacgtgaatt
     6481 agcacctatt cgtgaaagac gtttggagta cgctaaagat atgggagagg tgttccgtat
     6541 gttacaagaa ggtagtcaaa aagcaagaac tgtggcagcc aagactttat cagaagtgaa
     6601 gtcagcaatg ggtattaatt atttttaatg ttttagagaa aaagctaagt tagtaatgat
     6661 aagataatta ctattatcaa agcaggacct aatttgtcat ttcttta
//
LOCUS       DATXQK010000066         6667 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.066, whole genome shotgun sequence.
ACCESSION   DATXQK010000066 DATXQK010000000
VERSION     DATXQK010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6667
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.066"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            120..4226
                     /gene="cas9"
                     /locus_tag="VC394_001563"
     CDS             120..4226
                     /gene="cas9"
                     /locus_tag="VC394_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922251.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
                     /protein_id="HEP5995018.1"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDDYKVPSKKFKVLGNTDRHSIK
                     KNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE
                     ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAH
                     MIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSK
                     SRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLD
                     NLLAQIGDQYADLFLAAKNLSDAILLSDILRLNSEITKAPLSASMIKRYDEHHQDLTL
                     LKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLAK
                     LNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY
                     YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVL
                     PKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKE
                     DYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLT
                     LFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDF
                     LKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQT
                     VKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKE
                     HPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFIKDDSIDNKV
                     LTRSDKNRGKSNNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKA
                     GFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQF
                     YKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIG
                     KATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLS
                     MPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVV
                     AKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVRKDLIVKLPKYSLF
                     ELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQH
                     KHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGA
                     PAAFKYFDTTIDRKRYTSTKEVLDATFIHQSITGLYETRIDLSQLGGD"
     gene            4226..5095
                     /gene="cas1"
                     /locus_tag="VC394_001564"
     CDS             4226..5095
                     /gene="cas1"
                     /locus_tag="VC394_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HEP5995019.1"
                     /translation="MAGWRTVVVNTHSKLSYKNNHLIFKDAYKTELIHLSEIDILLLE
                     TTDIVLSTMLVKRLVDENVLVIFCDDKRLPTAMLMPFYGRHDSSLQLGKQMSWSETVK
                     SQVWTTIIAQKILNQSCYLGACSYFEKSQSIMDLYHGLENFDPSNREGHAARIYFNTL
                     FGNDFSRDLEHPINAGLDYGYTLLLSMFAREVVVSGCMTQFGLKHANQFNQFNFASDI
                     MEPFRPLVDKIVYENRNQPFPKIKRELFTLFSDTFSYNGKEMYLTNIISDYTKKVVKA
                     LNNEGKGVPEFRI"
     gene            5092..5433
                     /gene="cas2"
                     /locus_tag="VC394_001565"
     CDS             5092..5433
                     /gene="cas2"
                     /locus_tag="VC394_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEP5995020.1"
                     /translation="MSYRYMRMILMFDMPTDTAEERKAYRKFRKFLLSEGFIMHQFSI
                     YSKLLLNNTANNAMIGRLREHNPNKGNITLLTVTEKQFARMIYLHGERNNCIANSDER
                     LVFLGEAFDES"
     gene            5423..6085
                     /gene="csn2"
                     /locus_tag="VC394_001566"
     CDS             5423..6085
                     /gene="csn2"
                     /locus_tag="VC394_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II-A CRISPR-associated protein Csn2"
                     /protein_id="HEP5995021.1"
                     /translation="MNLNFSLLDEPIPLRGGTILVLEDVCVFSKIVQYCYKYEEDSEL
                     KFFDRKMKTIKESEIMLVTDILGFDVNSSTILKLIHADLESQFNEKPEVKSMIDKLVA
                     TITELIVFECLENELDLEYDEITILELIKSLGVKVETQSDTIFEKCLEILQIFKYLTK
                     KKLLIFVNSGAFLTKDEVASLQEYISLTNLTVLFLEPRELYDFPQYILDEDYFLITKN
                     MV"
     repeat_region   6188..6487
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=6188..6223
                     /rpt_unit_seq="gttttagagctatgctgttttgaatggtcccaaaac"
BASE COUNT         2298 a          982 c         1315 g         2072 t
ORIGIN      
        1 tattttgaaa aagaaaaatt aaagcatatt aaaccaattt cggaggtcat taaaactatt
       61 agtgaaatca tcaaactcat tatggattta atttaaactt tttattttag gaggcaaaaa
      121 tggataagaa atactcaata ggcttagata tcggcacaaa tagcgtcgga tgggcggtga
      181 tcactgatga ttataaggtt ccgtctaaaa agttcaaggt tctgggaaat acagaccgcc
      241 acagtatcaa aaaaaatctt ataggggctc ttttatttga cagtggagag acagcggaag
      301 cgactcgtct caaacggaca gctcgtagaa ggtatacacg tcggaagaat cgtatttgtt
      361 atctacagga gattttttca aatgagatgg cgaaagtaga tgatagtttc tttcatcgac
      421 ttgaagagtc ttttttggtg gaagaagaca agaagcatga acgtcatcct atttttggaa
      481 atatagtaga tgaagttgct tatcatgaga aatatccaac tatctatcat ctgcgaaaaa
      541 aattggtaga ttctactgat aaagcggatt tgcgcttaat ctatttggcc ttagcgcata
      601 tgattaagtt tcgtggtcat tttttgattg agggagattt aaatcctgat aatagtgatg
      661 tggacaaact atttatccag ttggtacaaa cctacaatca attatttgaa gaaaacccta
      721 ttaacgcaag tggagtagat gctaaagcga ttctttctgc acgattgagt aaatcaagac
      781 gattagaaaa tctcattgct cagctccccg gtgagaagaa aaatggctta tttgggaatc
      841 tcattgcttt gtcattgggt ttgaccccta attttaaatc aaattttgat ttggcagaag
      901 atgctaaatt acagctttca aaagatactt acgatgatga tttagataat ttattggcgc
      961 aaattggaga tcaatatgct gatttgtttt tggcagctaa gaatttatca gatgctattt
     1021 tactttcaga tatcctaaga ttaaatagtg aaataactaa ggctccccta tcagcttcaa
     1081 tgattaaacg ctacgatgaa catcatcaag acttgactct tttaaaagct ttagttcgac
     1141 aacaacttcc agaaaagtat aaagaaatct tttttgatca atcaaaaaac ggatatgcag
     1201 gttatattga tgggggagct agccaagaag aattttataa atttatcaaa ccaattttag
     1261 aaaaaatgga tggtactgag gaattattgg cgaaactaaa tcgtgaagat ttgctgcgca
     1321 agcaacggac ctttgacaac ggctctattc cccatcaaat tcacttgggt gagctgcatg
     1381 ctattctgag aagacaagaa gacttttatc catttttaaa agacaatcgt gagaagattg
     1441 aaaaaatctt gacttttcga attccttatt atgttggtcc attggcgcgt ggcaatagtc
     1501 gttttgcatg gatgactcgg aagtctgaag aaacaattac cccatggaat tttgaagaag
     1561 ttgtcgataa aggtgcttca gctcaatcat ttattgaacg catgacaaac tttgataaaa
     1621 atcttccaaa tgaaaaagta ctaccaaaac atagtttgct ttatgagtat tttacggttt
     1681 ataacgaatt gacaaaggtc aaatatgtta ctgagggaat gcgaaaacca gcatttcttt
     1741 caggtgaaca gaagaaagcc attgttgatt tactcttcaa aacaaatcga aaagtaaccg
     1801 ttaagcaatt aaaagaagat tatttcaaaa aaatagaatg ttttgatagt gttgaaattt
     1861 caggagttga agatagattt aatgcttcat taggtaccta ccatgatttg ctaaaaatta
     1921 ttaaagataa agattttttg gataatgaag aaaatgaaga tatcttagag gatattgttt
     1981 taacattgac cttatttgaa gatagggaga tgattgagga aagacttaaa acatatgctc
     2041 acctctttga tgataaggtg atgaaacagc ttaaacgtcg ccgttatact ggttggggac
     2101 gtttgtctcg aaaattgatt aatggtatta gggataagca atctggcaaa acaatattag
     2161 attttttgaa atcagatggt tttgccaatc gcaattttat gcagctgatc catgatgata
     2221 gtttgacatt taaagaagac attcaaaaag cacaagtgtc tggacaaggc gatagtttac
     2281 atgaacatat tgcaaattta gctggtagcc ctgctattaa aaaaggtatt ttacagactg
     2341 taaaagttgt tgatgaattg gtcaaagtaa tggggcggca taagccagaa aatatcgtta
     2401 ttgaaatggc acgtgaaaat cagacaactc aaaagggcca gaaaaattcg cgtgagcgta
     2461 tgaaacgtat tgaagaaggt atcaaagaat taggaagtca gattcttaaa gagcatcctg
     2521 ttgaaaatac tcaattgcaa aatgaaaagc tctatctcta ttatctccaa aatggaagag
     2581 acatgtatgt ggaccaagaa ttagatatta atcgtttaag tgattatgat gtcgatcaca
     2641 ttgttccaca aagtttcatt aaagacgatt caatagacaa taaggtctta acgcgttctg
     2701 ataaaaatcg tggtaaatcg aataacgttc caagtgaaga agtagtcaaa aagatgaaaa
     2761 actattggag acaacttcta aacgccaagt taatcactca acgtaagttt gataatttaa
     2821 cgaaagctga acgtggaggt ttgagtgaac ttgataaagc tggttttatc aaacgccaat
     2881 tggttgaaac tcgccaaatc actaagcatg tggcacaaat tttggatagt cgcatgaata
     2941 ctaaatacga tgaaaatgat aaacttattc gagaggttaa agtgattacc ttaaaatcta
     3001 aattagtttc tgacttccga aaagatttcc aattctataa agtacgtgag attaacaatt
     3061 accatcatgc ccatgatgcg tatcttaatg ccgtcgttgg aactgctttg attaagaaat
     3121 atccaaaact tgaatcggag tttgtctatg gtgattataa agtttatgat gttcgtaaaa
     3181 tgattgctaa gtctgagcag gaaataggca aagcaaccgc aaaatatttc ttttactcta
     3241 atatcatgaa cttcttcaaa acagaaatta cacttgcaaa tggagagatt cgcaaacgcc
     3301 ctctaatcga aactaatggg gaaactggag aaattgtctg ggataaaggg cgagattttg
     3361 ccacagtgcg caaagtattg tccatgcccc aagtcaatat tgtcaagaaa acagaagtac
     3421 agacaggcgg attctccaag gagtcaattt taccaaaaag aaattcggac aagcttattg
     3481 ctcgtaaaaa agactgggat ccaaaaaaat atggtggttt tgatagtcca acggtagctt
     3541 attcagtcct agtggttgct aaggtggaaa aagggaaatc gaagaagtta aaatccgtta
     3601 aagagttact agggatcaca attatggaaa gaagttcctt tgaaaaaaat ccgattgact
     3661 ttttagaagc taaaggatat aaggaagtta gaaaagactt aatcgttaaa ctacctaaat
     3721 atagtctttt tgagttagaa aacggtcgta aacggatgct ggctagtgcc ggagaattac
     3781 aaaaaggaaa tgagctggct ctgccaagca aatatgtgaa ttttttatat ttagctagtc
     3841 attatgaaaa gttgaagggt agtccagaag ataacgaaca aaaacaattg tttgtggagc
     3901 agcataagca ttatttagat gagattattg agcaaatcag tgaattttct aagcgtgtta
     3961 ttttagcaga tgccaattta gataaagttc ttagtgcata taacaaacat agagacaaac
     4021 caatacgtga acaagcagaa aatattattc atttatttac gttgacgaat cttggagctc
     4081 ccgctgcttt taaatatttt gatacaacaa ttgatcgtaa acgatatacg tctacaaaag
     4141 aagttttaga tgccactttt atccatcaat ccatcactgg tctttatgaa acacgcattg
     4201 atttgagtca gctaggaggt gactgatggc tggttggcgt actgttgtgg taaataccca
     4261 ctcgaaatta tcctataaga ataatcatct gatttttaag gatgcctata aaacggagct
     4321 gatccattta tcagaaattg atattttgtt attagaaacg accgatattg tcttgtccac
     4381 tatgctggta aaacggctag tggatgagaa tgtccttgtc atattctgtg atgataaacg
     4441 attaccaaca gctatgctga tgccttttta tggtcgtcat gattcgagtt tacagcttgg
     4501 gaaacaaatg tcctggtcag aaacagtcaa atcgcaggtt tggacgacga ttattgctca
     4561 aaagattttg aatcaatctt gctatctagg agcatgctcc tattttgaaa aatcccaatc
     4621 tattatggat ttatatcatg gtttggaaaa ttttgatccg agtaatcgag aagggcatgc
     4681 agcgagaatt tattttaata cactttttgg gaacgatttc tcaagagatt tggagcatcc
     4741 aatcaatgca ggtctggatt atggttatac tttattattg agtatgtttg cgcgtgaagt
     4801 ggttgtgtct ggatgtatga ctcagtttgg gcttaaacac gctaatcagt ttaatcagtt
     4861 caattttgct agcgatatta tggaaccatt taggcctttg gtggataaga ttgtttatga
     4921 aaatcgaaat cagccttttc ccaaaataaa gagagagtta tttactttgt tttcagatac
     4981 attttcatat aatggtaaag agatgtatct cacgaatatt attagcgatt atactaaaaa
     5041 agttgtcaaa gctctgaata atgaagggaa aggagttcct gaatttagga tatgagttat
     5101 agatatatga gaatgatact tatgtttgat atgccgacgg acaccgctga ggaacgaaaa
     5161 gcctatcgaa aatttcggaa atttttactt agtgaagggt ttatcatgca tcaattttct
     5221 atttatagta agttgctgtt gaataataca gctaacaatg ccatgattgg tcggctgagg
     5281 gagcataatc ctaataaagg aaatattaca ttactaacgg tcacagaaaa acagtttgca
     5341 cgaatgattt atttacatgg tgaaagaaat aattgtattg caaactccga tgaaagactt
     5401 gtatttcttg gggaggcttt tgatgaatct taatttttcc ttactagatg aaccgattcc
     5461 attaagaggc ggtacaattc ttgtgctcga agatgtctgt gtattttcaa aaatagtgca
     5521 atattgttac aaatatgagg aagattctga acttaaattt tttgatcgca agatgaaaac
     5581 catcaaagaa tcagaaatca tgcttgtaac agatatttta ggatttgatg ttaactcctc
     5641 aaccatttta aaattgattc atgcagattt agaatctcaa tttaatgaga aacccgaagt
     5701 gaaatcgatg attgacaaat tggttgctac gattacagaa ctgattgtct ttgaatgctt
     5761 agaaaatgaa ttagatttag agtatgatga aatcacaatc ctggaattga ttaagtcctt
     5821 aggagtaaaa gtagaaacgc aaagtgatac tatttttgaa aaatgtctag agatacttca
     5881 aattttcaaa tatctcacta agaaaaagtt gcttattttt gtcaatagcg gagcttttct
     5941 aacaaaggat gaagtggcta gtttacaaga gtatatatca ttgacaaatt taacagttct
     6001 ctttttagaa ccacgtgaac tatatgattt tccgcagtat attttagatg aagattattt
     6061 cttaataact aaaaatatgg tataatactc ttaataaatg cagtaataca ggggcttttc
     6121 aagactgaag tctagctgag acaaatagtg cgattacgaa attttttaga caaaaatagt
     6181 ctacgaggtt ttagagctat gctgttttga atggtcccaa aactgctcca gatggatttt
     6241 taaactgatt attgttttag agctatgctg ttttgaatgg tcccaaaact agaaattgac
     6301 aatgcttttt cttttgtctg ttttagagct atgctgtttt gaatggtccc aaaacaatag
     6361 agctagacaa aaaattgagt ttgacgtttt agagctatgc tgttttgaat ggtcccaaaa
     6421 cgacatatta acgtcctttc tcctgctttc cgttttagag ctatgctgtt ttgaatggtc
     6481 tccattcaac attgccgatg ataacttgag aaagagggtt aataccagca gtcggatacc
     6541 cacgccaaac gcatgaagaa acttttgttt tcagaaaact agattcataa tgacaaaatc
     6601 aatcaaagta gtacactata gatgaggtga ctacgatgat tgattttatt atttctattg
     6661 atgattg
//
LOCUS       DATXQK010000067         6112 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.067, whole genome shotgun sequence.
ACCESSION   DATXQK010000067 DATXQK010000000
VERSION     DATXQK010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6112
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.067"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            129..401
                     /gene="rpsP"
                     /locus_tag="VC394_001567"
     CDS             129..401
                     /gene="rpsP"
                     /locus_tag="VC394_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEP5995022.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRAPRDGRFIETVGTYNPLV
                     AENQITIKEDRVLEWLSKGAQPSDTVRNILSKAGVMAKFHDQKFSK"
     gene            411..650
                     /locus_tag="VC394_001568"
     CDS             411..650
                     /locus_tag="VC394_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEP5995023.1"
                     /translation="MDTIENLIIAIVKPLISQPDNLTIKIEDTPDFLEYHLDLDAQDI
                     GRVIGKKGRTITAIRSIVYSVPTLGKKVRLVIDEK"
     gene            1582..4608
                     /locus_tag="VC394_001569"
     CDS             1582..4608
                     /locus_tag="VC394_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HEP5995024.1"
                     /translation="MKKHLKTVALTLTTVSVVTHNQEVFSLVKEPILKQTQASSSISG
                     ADYAESSGKSKLKINETSGPVDDTVTDLFSDKRTTPEKIKDNLAKGPREQELKAVTEN
                     TESEKQITSGSQLEQSKESLSLNKTVPSTSNWEICDFITKGNTLVGLSKSGVEKLSQT
                     DHLVLPSQAADGTQLIQVASFAFTPDKKTAIAEYTSRAGENGEISQLDVDGKEIINEG
                     EVFNSYLLKKVTIPTGYKHIGQDAFVDNKNIAEVNLPESLETISDYAFAHLALKQIDL
                     PDNLKAIGELAFFDNQITGKLSLPRQLMRLAERAFKSNHIKTIEFRGNSLKVIGEASF
                     QDNDLSQLMLPDGLEKIESEAFTGNPGDDHYNNRVVLWTKSGKNPYGLATENTYVNPD
                     KSLWQESPEIDYTKWLEEDFTYQKNSVTGFSNKGLQKVKRNKNLEIPKQHNGVTITEI
                     GDNAFRNVDFQNKTLRKYDLEEVKLPSTIRKIGAFAFQSNNLKSFEASDDLEEIKEGA
                     FMNNRIETLELKDKLVTIGDAAFHINHIYAIVLPESVQEIGRSAFRQNGANNLIFMGS
                     KVKTIGEMAFLSNRLEHLDLSEQKQLTEIPVQAFSDNALKEVLLPASLKTIREEAFKK
                     NHLKQLEVASALSHIAFNALDDNDGDEQFDNKVVVKTHHNSYALADGEHFIVDPDKLS
                     STMVDLEKILKLIEGLDYSTLRQTTQTQFRDMTTAGKALLSKSNLRQGEKQKFLQEAQ
                     FFLGRVDLDKAIAKAEKALVTKKATKNGQLLERSINRAVLAYNNSAIKKANVKRLEKE
                     LDLLTGLVEGKGPLAQATMVQGVYLLKTPLPLPEYYIGLNVYFDKSGKLIYALDMSDT
                     IGEGQKDAYGNPILNVDEDNEGYHALAVATLADYEGLDIKTILNSKLSQLTSIRQVPT
                     AAYHRAGIFQAIQNAAAEAEQLLPKPGTHSEKSSSSESANSKDRGLQSNPKTNRGRHS
                     AILPRTGSKGSFVYGILGYTSVALLSLITAIKKKKY"
     gene            4628..5029
                     /locus_tag="VC394_001570"
     CDS             4628..5029
                     /locus_tag="VC394_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEP5995025.1"
                     /translation="MITQEMKDLINNQLAMVATVDAKGQPNIGPKRSMRLWDDKTFIY
                     NENTDGQTRINIEDNGKIEIAFVDRERLLGYRFVGTAEIQTEGAYYEAAKKWAQGRMG
                     VPKAVGIIHVERIFNLQSGANAGKEINSESN"
     gene            complement(5219..6094)
                     /locus_tag="VC394_001571"
     CDS             complement(5219..6094)
                     /locus_tag="VC394_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEP5995026.1"
                     /translation="MPASKKVTIIFILNLSFSLIEFIFGTLFFSGAILADAVHDFGDA
                     IAIGISAILERKAVKKESPNFSLGYKRFSLLGALTTNLILISGSLLVMIETIPKLWHP
                     TIVNYDGMFVLAIFAIIINGFASFIIHSNQTKNEEILSLHFLEDILGWLAIIILSLIL
                     KWKPWYILDPLLSIAIASFILSKALPKLVATANIFLDGVPDSIDYCALHHELSQLPHI
                     VSVNQLNVWSMDGIDHRATIHCCLKESTTEKHCKKSIRLICQRYNINSVTVEIDTSLN
                     EHQHHCSSLSGIEVN"
BASE COUNT         2141 a          952 c         1145 g         1874 t
ORIGIN      
        1 aaaggtttaa agaaagtgtc aagaaaaaaa cttgacactt tctttaaact gagttatact
       61 gtttaacata ggaatttagc gtagctgtat ttcttataca ttactaaaag aaaagagact
      121 atttaaaaat ggcagtaaaa atccgtttaa ctcgtatggg ttctaagaaa aaacctttct
      181 accgtattaa tgttgcagat tcacgtgcac cacgtgatgg tcgttttatc gaaacagttg
      241 gaacatacaa cccattagtg gctgaaaatc aaattactat taaagaggat cgtgttcttg
      301 agtggttatc taaaggagcg cagccttctg ataccgttcg taacatcttg tctaaagctg
      361 gtgttatggc taagttccac gaccaaaaat tctctaaata ataacagcct atggatacca
      421 ttgaaaatct tattatcgct attgtgaaac ctttgatttc acaaccagat aatcttacca
      481 ttaaaattga agatactcct gactttttag agtatcatct tgacttggat gctcaagaca
      541 tcggtcgtgt tatcggcaaa aaaggtcgaa ctattactgc gataagatcg attgtctact
      601 cggttccaac actaggaaaa aaagttcgct tagttattga tgagaaataa tcactaactt
      661 gagatggatt gaaaagcttg ttgacttaaa gtcaacaagc tttttgaata gcaaaaatct
      721 taatatttct gaaatgaaaa gattagattt ttctgtcttt aactaaaaag aagttaaaga
      781 atggcttagc tgattgcctt tataagagat tatactggtt tttgatagct gctgtcatgt
      841 aaaatagcta caattcatct tacgagattg accttagcat tgccagtgca gtctttattt
      901 tctaacctgc tgtttgttgt atgtttatag tttcctaaaa aatgctctct cagttttttg
      961 ggggacattc ctaaaattaa gtatacttaa ttttaggaat gtttatattc tttacattct
     1021 gatgaaaata caaattatcc taacatgatg tattgaaagg agaaatagtt tatgttagta
     1081 tactgattaa gtgaagctgt aatgaggctt atacgatcaa aaaagttaga gtaactttat
     1141 aaaacagtgg tgttgaagca agtttagctt cttctcagaa atagattatc tataaaaaat
     1201 cccaaaagta gtttgtttcc agttaatgaa cttcttttgg gaaggctttt ttgctgatcg
     1261 ccacttcact tgtatatcgg gaacattcac gctaagatgt ataagttgaa tcgtttgtct
     1321 atggcttgat gaaaattaat caaaagataa tgttcaactc tataattagt acaagcaaaa
     1381 tgagataact tattcattag taagcataag atataacaag agaaattacc ttaaaacagg
     1441 aagtactaga gtatttaaaa taagtgatag acttggatat tattaggtcg actagtgttc
     1501 ttttcaataa tttaatatcg ttattattag tatcagccaa tcaaatgtgg ataagttggc
     1561 ttttagaaag gttttaccac aatgaagaaa catcttaaaa cagttgcctt gaccctcact
     1621 acagtatcgg tagtcaccca caatcaggaa gtttttagtt tagtcaaaga gccgattctt
     1681 aaacaaactc aagcttcttc atcgatttct ggcgctgact acgcagaaag tagcggtaaa
     1741 agcaagttaa agattaatga aacttctggc cctgttgatg atacagtcac tgacttattt
     1801 tcggataaac gtactactcc tgaaaaaata aaagataatc ttgctaaagg tccgagagaa
     1861 caagagttaa aggcagtaac agagaataca gaatcagaaa agcagatcac ttctggatct
     1921 caactagaac aatcaaaaga gtctctttct ttaaataaaa cagtgccatc aacgtctaat
     1981 tgggagattt gtgattttat tactaagggg aatacccttg ttggtctttc aaaatcaggt
     2041 gttgaaaagt tatctcaaac tgatcatctc gtattgccta gtcaagcagc agatggaact
     2101 caattgatac aagtagctag ttttgctttt actccagata aaaagacggc aattgcagaa
     2161 tataccagta gggctggaga aaatggggaa ataagccaac tagatgtgga tggaaaagaa
     2221 attattaacg aaggtgaggt ttttaattct tatctactaa agaaggtaac aatcccaact
     2281 ggttataaac atattggtca agatgctttt gtggacaata agaatattgc tgaggttaat
     2341 cttcctgaaa gcctcgagac tatttctgac tatgcttttg ctcacctagc tttgaaacag
     2401 atcgatttgc cagataattt aaaagcgatt ggagaattag ctttttttga taatcaaatt
     2461 acaggtaaac tttctttgcc acgtcagtta atgcgattag cagaacgtgc ttttaaatca
     2521 aaccatatca aaacaattga gtttagagga aatagtctaa aagtgatagg ggaagctagt
     2581 tttcaagata atgatctgag tcaactaatg ctacctgacg gtcttgaaaa aatagaatca
     2641 gaagctttta caggaaatcc aggagatgat cactacaata accgtgttgt tttgtggaca
     2701 aaatctggaa aaaatcctta tggtcttgct actgaaaata cctatgttaa tcctgataag
     2761 tcactatggc aggaaagtcc tgagattgat tatactaaat ggttagagga agattttacc
     2821 tatcaaaaaa atagtgttac aggtttttca aataaaggct tacaaaaagt aaaacgtaat
     2881 aaaaacttag aaattccaaa acagcacaat ggtgttacta ttactgaaat tggtgataat
     2941 gcttttcgca atgttgattt tcaaaataaa actttacgta aatatgattt ggaagaagta
     3001 aagcttccct caactattcg gaaaataggt gcttttgctt ttcaatctaa taacttgaaa
     3061 tcttttgaag caagtgacga tttagaagag attaaagagg gagcctttat gaataatcgt
     3121 attgaaacct tggaattaaa agataaatta gttactattg gtgatgcggc tttccatatt
     3181 aatcatattt atgccattgt tcttccagaa tctgtacaag aaatagggcg ttcagcattt
     3241 cggcaaaatg gtgcaaataa tcttattttt atgggaagta aggttaagac cataggtgag
     3301 atggcatttt tatcaaatag acttgaacat ctggatcttt ctgagcaaaa acagttaaca
     3361 gagattcctg ttcaagcctt ttcagacaat gccttgaaag aagtattatt accagcatca
     3421 ctgaaaacga ttcgagaaga agccttcaaa aagaatcatt taaaacaact ggaagtggca
     3481 tctgccttgt cccatattgc ttttaatgct ttagatgata atgatggtga tgaacaattt
     3541 gacaataaag tggttgttaa aacgcatcat aattcctacg cactagcaga tggtgagcat
     3601 tttatcgttg atccagataa gttatcttct acaatggtag accttgaaaa gattttaaaa
     3661 ctaatcgaag gtttagatta ttctacatta cgtcagacta ctcaaactca gtttagagac
     3721 atgactactg caggtaaagc gttgttgtca aaatctaacc tccgacaagg agaaaaacaa
     3781 aaattccttc aagaagcaca atttttcctt ggccgcgttg atttggataa agccatagct
     3841 aaagctgaga aggctttagt gaccaagaag gcaacaaaga atggtcagtt gcttgaaaga
     3901 agtattaaca gagcggtatt agcttataat aatagcgcta ttaaaaaagc taatgttaag
     3961 cgcttggaaa aagagttaga cttgctaaca ggattagttg agggaaaagg accattagcg
     4021 caagctacaa tggtacaagg agtttattta ttaaagacgc ctttgccatt gccagagtat
     4081 tatatcggat tgaacgttta ttttgacaag tctggaaaat tgatttatgc acttgatatg
     4141 agtgatacta ttggcgaggg acaaaaagac gcttatggta atcctatatt aaatgttgac
     4201 gaggataatg aaggttatca tgccttggca gttgccactt tagctgatta tgaggggctc
     4261 gacatcaaaa caattttaaa tagtaagctt agtcaattaa catctattcg tcaggtaccg
     4321 actgcagcct atcatagagc cggtattttc caagctatcc aaaatgcagc ggcagaagca
     4381 gagcagttat tgcctaaacc aggtacgcac tctgagaaat caagctcaag tgaatctgct
     4441 aactctaaag atagaggatt gcaatcaaac ccaaaaacga atagaggacg acactctgca
     4501 atattgccta ggacagggtc aaaaggcagc tttgtctatg gaatcttagg ttacactagc
     4561 gttgctttac tgtcactaat aactgctata aaaaagaaaa aatattaaat tgatttaagg
     4621 agaagaaatg ataacacaag aaatgaaaga tcttattaat aaccaattag caatggttgc
     4681 gacggttgat gccaaaggtc aacccaatat tggtccaaaa cgttctatgc gtctctggga
     4741 tgataaaaca tttatttata atgaaaatac agatggtcaa actcgtatta acattgaaga
     4801 taatgggaaa attgaaattg cctttgttga tcgtgagcgc cttttaggtt acaggtttgt
     4861 aggaactgct gaaattcaaa cagaaggtgc ttattacgaa gcagcgaaaa aatgggcaca
     4921 aggacgcatg ggggtaccaa aagcagttgg tattattcat gtcgagcgta tttttaattt
     4981 acaatctggt gctaatgcag gtaaagaaat aaacagtgaa agtaactaaa attaactaaa
     5041 attaactgta acagtattta ggacccttat tttaaatgct aaaaaacaag catactcttt
     5101 tttacagcat agtgactatg ttgtaaaaag gggtatgctt attaggtgct cttaatattt
     5161 ctagataact gcacctaata agttttcaat agacttatag cacattagtt gacttcaatt
     5221 agttgacttc aataccagaa agagaagaac agtgatgttg gtgttcgttt aaagaagtat
     5281 cgatttccac agtgactgag tttatattgt acctttgaca aatcagtcta atagattttt
     5341 tgcaatgttt ttcagtggta gattctttca agcagcaatg tatagttgct ctatggtcaa
     5401 taccatccat cgaccaaaca ttaagctgat taacagacac tatatgagga agttggctga
     5461 gttcatggtg caaagcgcaa tagtctatcg aatcaggaac accatctaaa aaaatgttag
     5521 cggttgctac taactttgga agagctttag ataatataaa agaagcaatg gcaattgata
     5581 ataaaggatc aagaatgtac caaggtttcc attttaagat cagtgacagt atgataatgg
     5641 ctaaccatcc aaggatatct tctaaaaagt gaaggcttaa tatttcttca ttttttgtct
     5701 ggttagagtg aatgatgaag ctagcaaatc cattgattat aattgcaaaa atagctaaaa
     5761 cgaacatacc gtcataatta acaatagtag gatgccataa ttttggtatc gtttcaatca
     5821 ttactaataa tgacccgcta ataagtatta gatttgtcgt tagcgctcct aaaaggctaa
     5881 atcgcttata gcctagtgaa aaatttggac tctctttttt aacagccttt ctttctaaga
     5941 tagctgagat accaatagca atggcatctc caaaatcgtg gacagcatct gctaaaatag
     6001 cacccgaaaa gaataatgtt ccaaaaataa attcaatgag ggaaaaacta agatttaata
     6061 taaaaataat ggtgactttt ttacttgctg gcataatttt ctccttttgt ta
//
LOCUS       DATXQK010000068         5396 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.068, whole genome shotgun sequence.
ACCESSION   DATXQK010000068 DATXQK010000000
VERSION     DATXQK010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5396
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.068"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            12..569
                     /gene="lepB"
                     /locus_tag="VC394_001572"
     CDS             12..569
                     /gene="lepB"
                     /locus_tag="VC394_001572"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEP5995027.1"
                     /translation="MVKRDFIRNILLLLIVIIGAILLRIFVFSTFKVSPETANTYLKS
                     GDLVTIKKNIQPKYKDFVVYRVGKKDYVSRVIAVEGDSVTYMDDIFYLNNMVESQAYL
                     EKMKAHYLNHAPFGTLYTDDFTVATITADKYQKVPKGKYLLLNDNRKNTNDSRRFGLI
                     NASQIKGLVTFRVLPLSDFGFVEVE"
     gene            745..2559
                     /gene="glmS"
                     /locus_tag="VC394_001573"
     CDS             745..2559
                     /gene="glmS"
                     /locus_tag="VC394_001573"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019804802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEP5995028.1"
                     /translation="MCGIVGVVGNRNATDILMQGLEKLEYRGYDSAGIFVANANQTNL
                     IKSVGRIADLRAKIGIDVAGSTGIGHTRWATHGQSTEDNAHPHTSQTGRFVLVHNGVI
                     ENYLHIKTEFLAGHDFKGQTDTEIAVHLIGKFVEEDKLSVLEAFKKALSIIEGSYAFA
                     LMDSQATDTIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETSEFMEIHDKELVILTKD
                     KVTVTDYDGKELIRDSYTAELDLSDIGKGTYPFYMLKEIDEQPTVMRQLISTYADETG
                     NVQVDPAIITSIQEADRLYILAAGTSYHAGFATKNMLEQLTDTPVELGVASEWGYHMP
                     LLSKKPMFILLSQSGETADSRQVLVKANAMGIPSLTVTNVPGSTLSREATYTMLIHAG
                     PEIAVASTKAYTAQIAALAFLAKAVGEANGKQEALDFNLVHELSLVAQSIEATLSEKD
                     LVAEKVQALLATTRNAFYIGRGNDYYVAMEAALKLKEISYIQCEGFAAGELKHGTISL
                     IEEDTPVIALISSSQLVASHTRGNIQEVAARGAHVLTVVEEGLDREGDDIIVNKVHPF
                     LAPIAMVIPTQLIAYYASLQRGLDVDKPRNLAKAVTVE"
     gene            2755..3090
                     /locus_tag="VC394_001574"
     CDS             2755..3090
                     /locus_tag="VC394_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEP5995029.1"
                     /translation="MSLPNCLQCQSEYVYEDGLLLICPMCAFEWTPGEEATEEEGLVV
                     LDSNGVRLSDGDTITVVKDLKVKGAPKDLKQGTRVKNIRLVEGDHNIDCKIDGFGAMK
                     LKSEFVKKI"
     gene            3197..3838
                     /locus_tag="VC394_001575"
     CDS             3197..3838
                     /locus_tag="VC394_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009854101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEP5995030.1"
                     /translation="MTYIQQVLPSLLDGALVTLQVFFIVIILSIPLGAILAFLMKIPF
                     KPLQWFLTLYVWMMRGTPLLLQLIFFYYVLPSVGISFDRMPAAILAFTLNYAAYFAEI
                     FRGGIEAIPKGQYEAAKVLKLKPLQTIRYIILPQVFKIVLPSVFNEVINLVKDSSLVY
                     VLGVGDLLLASKTAANRDATLAPMFIAGLIYLLLIGLVTIISKQVEKRFNYYQ"
     gene            3848..4477
                     /locus_tag="VC394_001576"
     CDS             3848..4477
                     /locus_tag="VC394_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009854102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEP5995031.1"
                     /translation="MLELKNISKQFGQKTIFDGFNLTVQDGEVLSLVGPSGGGKTTLL
                     RMLAGLESIDSGQVFYNGEDVGIDHLENRNLLGFVFQDFQLFPHLTVLDNLTLSPTIT
                     MGKQKSDAKEKALDLLARLGLKEHAQVYPYSLSGGQKQRVALARAMMIDPQIIGYDEP
                     TSALDPELRQTVEALIVQNREMGITQIVVTHDLVFAEAISDRIIRVNPK"
     gene            4493..5329
                     /locus_tag="VC394_001577"
     CDS             4493..5329
                     /locus_tag="VC394_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEP5995032.1"
                     /translation="MIIKKRTVAILAIASSFFLVACQATKGLKSGDAWGVYQKQKSIT
                     VGFDNTFVPMGYKDESGKCKGFDIDLAKEVFHQYGLKVNFQAINWDMKEAELNNGKID
                     VIWNGYSITKERQEKVAFTDSYMRNEQIIVVKKRSDIKTISDMKHKVLGAQSASSGYD
                     SLLRTPKLLKDFIKNKDANQYETFTQAFIDLKSDRIDGILIDKVYANYYLAKEGQLEN
                     YRMIPTTFENEAFSVGLRKEDKTLQAKINRAFRVLYQNGKFQAISEKWFGDDVATANI
                     KS"
BASE COUNT         1681 a          869 c         1138 g         1708 t
ORIGIN      
        1 taggagttaa tatggtaaag cgagatttta taagaaatat attattgtta ttaattgtga
       61 ttattggggc tattttgttg agaatctttg ttttttcaac ttttaaagtt tctccagaaa
      121 cagctaatac ttatttaaag agtggtgatt tagtcacaat caaaaaaaat attcagccca
      181 aatataaaga ttttgtggtt tatagagttg gaaaaaaaga ttatgtcagt cgagtcattg
      241 ctgttgaagg cgatagcgtg acttatatgg acgatatttt ttacctcaat aatatggtag
      301 agtcacaggc ttaccttgaa aagatgaaag cacattactt gaatcatgca ccgtttggca
      361 cattgtatac agatgatttt acagttgcta ccatcacagc tgataagtat caaaaagttc
      421 ctaaggggaa gtatcttctt ttgaatgata accggaaaaa tacgaatgac agtcgtcgat
      481 ttgggttaat aaatgcctcg cagattaaag gtttagtgac ctttagagtt ttgcctctca
      541 gcgattttgg atttgtagaa gtagagtagt tgtcaggcta ctctactttt tttataatta
      601 gactatacca attttatgtt tgacaagaca ttgtggatat tatatactgt aaacatactg
      661 tttttatcgg tataatatga ctataccaat tattgaaatt atgagagaaa gtagcaagtt
      721 aacgaacttg ttaaggtaat taagatgtgt ggaattgttg gagttgttgg aaatcgcaat
      781 gcaacggata ttttaatgca aggccttgaa aagcttgaat accggggtta tgattcagca
      841 ggaatttttg tggctaatgc caatcaaaca aacttgatta aatcagtggg gcggattgct
      901 gatttgcgtg ccaagattgg cattgatgtt gctggttcaa cagggattgg tcacacccgt
      961 tgggcaacgc atggccaatc aacagaggat aatgcccatc ctcacacgtc acaaactgga
     1021 cgttttgtac ttgttcataa tggtgtgatt gaaaattacc ttcacattaa aacagagttc
     1081 ctagctggac atgattttaa ggggcagaca gatactgaga ttgcagtaca cttgattgga
     1141 aaatttgtgg aagaagacaa gttgtcagta ctggaagctt ttaaaaaagc tttaagcatt
     1201 attgaaggtt cctacgcctt tgcgttaatg gatagccaag caactgatac tatttatgtg
     1261 gctaaaaaca agtctccatt gttgattgga cttggtgaag gttacaacat ggtttgttca
     1321 gatgccatgg ccatgattcg tgaaaccagt gaatttatgg aaattcatga taaggagcta
     1381 gttattttaa ccaaagataa ggtaactgtt acagactacg atggtaaaga gctgatacga
     1441 gattcctaca ctgctgaatt agacttatct gatattggca aagggactta tcctttctat
     1501 atgctgaaag aaattgatga gcaaccaacc gtaatgcgtc aattaatttc aacttatgca
     1561 gatgaaactg gtaacgtaca ggttgatcca gctatcatta cctctatcca agaggctgac
     1621 cgtctttata ttttagcggc agggacttcc taccatgctg gttttgcaac aaaaaatatg
     1681 cttgagcaat tgacagatac accagttgag ttgggagtgg cttctgagtg gggttaccac
     1741 atgcctctgc ttagcaagaa accaatgttt attctactaa gccaatcagg agaaaccgca
     1801 gatagtcgtc aagttttagt aaaggcaaat gctatgggta ttccgagttt gacagtaact
     1861 aacgttccag gatcaacctt atcacgtgaa gcaacataca ccatgttgat tcatgctgga
     1921 cctgaaattg ctgttgcgtc tacaaaagct tacactgcac aaattgctgc ccttgccttt
     1981 ttggctaagg cagttggtga ggcaaatggt aagcaagaag ctcttgactt taacttggta
     2041 catgagttgt cattggttgc ccaatctatt gaggcgactt tgtctgaaaa agatctcgtg
     2101 gcagaaaagg ttcaagcttt gctagctact actcgtaatg ctttttacat tgggcgtggc
     2161 aatgattatt acgttgcgat ggaagctgct ttgaaattaa aagagatttc ttatattcaa
     2221 tgcgaaggct ttgcggctgg tgaattgaaa catggaacca tttcattgat tgaggaggac
     2281 acgccagtaa tcgctttaat atcgtctagt cagttggttg cctctcatac gcgtggtaat
     2341 attcaagaag ttgctgcccg tggggctcat gttttaacag ttgtggaaga agggcttgac
     2401 cgtgagggag atgacattat tgtcaataag gttcatcctt tcctagcccc gattgctatg
     2461 gtcattccaa ctcaactgat tgcttactac gcttcattac aacgtggact tgatgttgat
     2521 aagccacgta atttggctaa agctgtaaca gtagaataaa tgattataat ggataaggca
     2581 accatctgtt aggtggttgc tttttgtttt taggagaata gcagagctta gaaaatggcc
     2641 ttgagaaaag ctatacaaga agaatagtca tcttctgatg agaacgtatt catcttgtca
     2701 aatcattgat tctaaagtaa aatggtaaca ttaagaaatg tttaggagtg taagatgtca
     2761 ttaccaaatt gtttgcaatg ccagtctgaa tatgtttacg aagatggtct tttattaatc
     2821 tgtccaatgt gtgcttttga atggacacca ggcgaagaag cgactgaaga agaaggactt
     2881 gttgttttag atagcaatgg tgtccgactg tctgatggtg ataccattac tgtggttaaa
     2941 gatttgaaag taaaaggcgc tccaaaagac ctgaaacaag ggactcgtgt taaaaatatt
     3001 cgcctggtgg aaggtgatca taacattgat tgtaagattg atgggtttgg cgcgatgaaa
     3061 ttgaaatcag aatttgtcaa gaaaatctaa taataatacg caaccagctg atctggttgt
     3121 atttttataa aaaaacctct ccccaaatac gttcaaattt ggtataatag taaaatattc
     3181 agttttagga ggcaacatga cctatattca gcaagtttta cccagcttat tagatggtgc
     3241 cttggtgacc ttacaagtat tctttattgt tatcattctt tctataccct taggggctat
     3301 tttagctttc ttgatgaaga ttccctttaa accgctacag tggtttttga ccctatacgt
     3361 gtggatgatg cgagggacac cattactact tcaattgatt tttttctatt atgttttgcc
     3421 aagtgtgggg attagttttg atcgaatgcc agctgctatt ttggcgttta ctttgaatta
     3481 tgctgcctac tttgctgaaa tttttagagg tgggattgag gctattccaa aaggtcaata
     3541 tgaagcagct aaagtattaa agttaaaacc tcttcaaacc attcgttata ttattttgcc
     3601 tcaagtgttt aaaattgtgt taccaagtgt tttcaatgaa gtcattaatt tggtcaaaga
     3661 ttcttccctt gtctatgtac tcggtgtagg agatctttta ttagcaagta agacggcagc
     3721 caatagggat gcaaccttag cccctatgtt tatcgctggt cttatctatt tgcttttaat
     3781 tggactggtc acgattattt caaaacaagt tgaaaaacgg tttaattatt atcagtaagg
     3841 aggcttcatg ttagaactca aaaatatttc caaacagttt ggtcaaaaaa ccatttttga
     3901 tggctttaat ctaaccgttc aagacggaga agtgctctct ttggtaggcc catcaggtgg
     3961 cggtaagaca acactcttac ggatgttggc tggtcttgaa tctattgatt caggtcaagt
     4021 cttttacaat ggcgaagatg ttggtattga tcatttggaa aatcgtaact tgcttggatt
     4081 tgtttttcaa gattttcaat tgtttcctca tcttaccgtt ttagacaacc taacgctgtc
     4141 tccaacaatt acaatgggca agcaaaaatc tgacgctaaa gaaaaggctt tggatttact
     4201 ggcacgttta ggattaaaag aacatgccca agtttatccc tattcgttat ctggaggtca
     4261 aaaacaacgg gttgctttgg caagagccat gatgattgac cctcaaatta tcggttacga
     4321 tgaaccgacc agtgctctag atcctgaatt acgtcagacg gtagaagcct tgattgttca
     4381 aaaccgtgaa atgggaataa ctcaaattgt agtcactcac gatcttgttt ttgccgaagc
     4441 catctcagat cgtattatta gggttaatcc taaatagaag aggacccaaa agatgattat
     4501 aaaaaaaaga accgtagcaa ttttagccat agctagtagc tttttcttgg tagcttgtca
     4561 agctactaaa ggtcttaaat caggagatgc ttggggagtt taccaaaagc aaaaaagtat
     4621 tacagttggt tttgacaata cgtttgttcc tatgggctat aaggatgaaa gcggcaaatg
     4681 caaaggtttt gatattgatt tggctaaaga agtttttcac caatatggac tcaaggttaa
     4741 ctttcaagct attaattggg acatgaaaga agcagaacta aacaatggta aaattgatgt
     4801 aatctggaat ggttattcaa taactaagga gcgtcaggaa aaggttgcct ttactgattc
     4861 ttacatgaga aatgaacaaa ttattgttgt caaaaaaaga tctgatatta aaacaatatc
     4921 agatatgaaa cataaagtgt taggagcaca atcagcttca tcaggttatg actccttgtt
     4981 aagaactcct aaactgctga aagattttat taaaaataaa gacgctaatc aatatgaaac
     5041 ctttacacaa gcttttattg atttaaaatc agatcgtatc gatggaatat tgattgacaa
     5101 agtatatgcc aattactatt tagcaaaaga agggcaattg gagaattatc ggatgatccc
     5161 aacgaccttt gaaaatgaag cattttcggt tggacttaga aaagaagaca aaacgttgca
     5221 agcaaaaatt aatcgtgctt tcagggtgct ttatcaaaat ggcaaatttc aagctatttc
     5281 tgagaaatgg tttggagatg atgttgccac tgccaatatt aaatcttaaa aaggacactt
     5341 gtcctttttt tgtttaacag gtgagatcta gcagacaaag tcagctagat gattta
//
LOCUS       DATXQK010000069         5369 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.069, whole genome shotgun sequence.
ACCESSION   DATXQK010000069 DATXQK010000000
VERSION     DATXQK010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5369
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.069"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            65..586
                     /locus_tag="VC394_001578"
     CDS             65..586
                     /locus_tag="VC394_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEP5995033.1"
                     /translation="MTKDRQIKKTKTAIYSAFLALLQKKEYSKITVRDMITLANVGRS
                     TFYAHYESKEMLLKELCEELFHHLFRQKRNVTFEDYLVHILKHFEQNKDSIATLLLSN
                     DPYFLLRFKNELEHDVYPNLRCKYIDKTTIPEVFLKQFVLSSFIETLKWWLHQRQRMS
                     ANELLKYYLELIK"
     gene            801..1319
                     /gene="rimM"
                     /locus_tag="VC394_001579"
     CDS             801..1319
                     /gene="rimM"
                     /locus_tag="VC394_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEP5995034.1"
                     /translation="MEYFNVGKIVNTQGLQGEMRVLSVSDFAEERFKKGSQLALFDDK
                     DQFVQEVTIVSHRKQKHFDIIKLKDMYHINAIEKYKGYTLKVSKANQGDLQEGEFYYH
                     QIIGMAVYEKDRLIGYVKEILQPGANDVWVVKRQGKRDLLLPYIPPVVLNVDVPNKRV
                     DVELMEGLDDED"
     gene            1309..2040
                     /gene="trmD"
                     /locus_tag="VC394_001580"
     CDS             1309..2040
                     /gene="trmD"
                     /locus_tag="VC394_001580"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEP5995035.1"
                     /translation="MKIDILTLFPEMFAPLEHSIVGKAKEKGLLDIHYHNFRDYAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDTIEQIEAKKPRIILLDPAGKPFTQAYAEELALEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDFVLTGGELAAMTMVDATVRLIPQVLGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMTVPDVLMSGHHERIRLWRLEESLRKTYLRRPDLL
                     ERYDFSEEERKLLDKIKEALGQGED"
     gene            2040..3032
                     /locus_tag="VC394_001581"
     CDS             2040..3032
                     /locus_tag="VC394_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEP5995036.1"
                     /translation="MKDKAYDITIIGGGPIGLFAAFYAGLRGVTVKIIESLSELGGQP
                     AILYPEKMIYDIPAYPSLTGVELTENLIKQLNRFEDRITICLKEEVLTFDKVKGGFSI
                     RTNKAEHFSKAIIIACGNGAFAPRTLGLESEENFADHNLFYNVHQLDQFAGQKVVICG
                     GGDSAVDWALALEDIAESVTVVHRRDAFRAHEHSVELLTTSTVNLLTPYVPKALKGIG
                     NLAEKLVIQKVKEDEVLELELDSLIVSFGFSTSNKNLKNWNLDYKRSSITVSPLFQTS
                     QEGIFAIGDAAAYNGKVDLIATGFGEAPTAVNQAINYIYPDRDNRVVHSTSLID"
     gene            3211..4269
                     /locus_tag="VC394_001582"
     CDS             3211..4269
                     /locus_tag="VC394_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HEP5995037.1"
                     /translation="MTTTDKETFSSFMNKVLAGTAIAIVVALIPNAILATFLKPLLPN
                     MAAAEFLHIVQVFQFFTPIMAGFLIGQQFKFNPMQQLAVGGAAYIGSGAWAYTEVIQK
                     GVATGTFQLRGIGDLINMMITASLAVLAVKYFGNKFGSLTIILLPITIGTGVGYIGWK
                     FLPYVSYVTTLIGQGINSFTTLQPILMSILIAVAFSLIIVSPISTVAIGLAIGLNGMA
                     AGAASMGIASTAAVLVWATLKVNKSGVPIAIALGAMKMMMPNFLKHPIMAIPMVFTAA
                     ISSLTVPLFNLVGTPASSGFGLVGAVGPIASLAGGSSILIIILAWIIVPFAVAFAAHK
                     VSKDILKLYKEDIFVFEG"
     gene            4282..5205
                     /locus_tag="VC394_001583"
     CDS             4282..5205
                     /locus_tag="VC394_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEP5995038.1"
                     /translation="MLVYIAGSGAMGCRFGYQISKTNNDVILLDNWEDHINAIKENGL
                     VVTGDVEETVKLPIMKPTEATQEADLIILFTKAMQLPQMLQDIKGIIGKETKVLCLLN
                     GLGHEDVIRQYIPEHNILMGVTVWTAGLEGPGRAHLQGVGALNLQSMDPNNQDAGHQV
                     ADLLNKANLNATYDENVVPNIWRKACVNGTMNSTCALLDCTIGELFASEDGLKMVKEI
                     IHEFVIVGQAEGVELNEEEITQYVMDTSVKAAHHYPSMHQDLVQNHRLTEIDFINGAV
                     NTKGEKLGINTPYCRMITELVHAKEAVLNIQ"
BASE COUNT         1700 a          857 c         1150 g         1662 t
ORIGIN      
        1 acaattacta taaccaaaaa tgagcacctg ttcaatagtg aacaacattt agaaagtgtc
       61 cgatatgaca aaagacagac aaatcaaaaa aacaaaaaca gctatctata gtgcttttct
      121 tgctttatta caaaaaaaag aatattcgaa aataactgtt cgagatatga ttacacttgc
      181 taatgtaggg agatcaacct tttatgcaca ttatgaaagc aaagaaatgt tactaaaaga
      241 actctgtgaa gaactttttc atcatttgtt taggcaaaaa agaaatgtga cttttgagga
      301 ctatttggta catattttaa agcattttga acaaaataaa gacagcattg cgacgttgtt
      361 actttctaat gatccctatt ttttattaag atttaaaaac gaactagaac atgatgttta
      421 ccctaacttg cgctgcaagt atatcgacaa aacgactatt ccagaagtat tcctgaaaca
      481 atttgttcta tctagtttta tagaaacctt aaaatggtgg ctccatcaaa ggcaaaggat
      541 gtctgcaaat gaactattaa agtattattt agagctcata aaataatgac tagtgagtca
      601 tcttgccaaa ttcaattttt taatacagac ggattttaac aatttaacag agaaagacaa
      661 aactgtaagt gcaaaataga ctataacgtg ataaaataat aggtaatttg gttctgaaaa
      721 agaagtgtct atcaaacttt agaatcaaga gaggtcgcta aagtgacgtg agataaagag
      781 ttaacgattt aggagctacc atggaatatt ttaatgttgg aaaaattgtc aacacgcaag
      841 gcctgcaagg agagatgcgc gtgttgtctg tcagtgattt tgctgaggag cgctttaaga
      901 agggatcgca attggctttg tttgatgaca aagaccagtt tgttcaagaa gtgacaattg
      961 ttagtcaccg taaacaaaag cattttgata ttatcaaatt gaaagacatg tatcatatta
     1021 atgcgattga aaaatataag ggctatacgt taaaagtgtc aaaagctaat caaggagacc
     1081 tgcaagaagg agaattttac taccatcaga ttatcggtat ggctgtttat gaaaaggacc
     1141 ggttgattgg ctatgtcaag gaaatcttgc agccaggtgc taatgatgtt tgggtagtga
     1201 aacgccaagg aaaacgagac ttgctcttac catatattcc cccagtagtt cttaacgtag
     1261 atgttcctaa caagcgcgta gatgttgaat tgatggaagg cttagacgat gaagattgat
     1321 attttaaccc tttttcctga aatgtttgcc cccctagaac attccattgt aggcaaagca
     1381 aaagaaaaag gattacttga cattcactac cataactttc gagattatgc tgaaaaagct
     1441 cgtcatgtgg atgacgaacc ctatggtggg ggccaaggca tgttattgag agcacagccc
     1501 atttttgaca ccattgaaca gattgaagcc aaaaaaccaa ggattattct tttagatcca
     1561 gcaggtaaac cctttactca ggcttatgct gaagaattag ctcttgagga agagttaatt
     1621 tttatttgtg gtcattatga aggttatgat gagcggatta agaccttggt gacggatgaa
     1681 atttcattag gtgactttgt attaacagga ggtgagttgg cggccatgac tatggtggat
     1741 gcgactgtca ggttaattcc acaggtactt gggaaagaaa gtagccatca ggacgattct
     1801 ttttcatcag gacttttaga ataccctcaa tacacaagac cttatgatta tcgtggtatg
     1861 acagttccag atgttctaat gagtggtcat cacgaacgta ttcgcttgtg gcgtctagaa
     1921 gaaagtctca gaaaaaccta tttgagaagg ccagatttgt tagagcggta cgatttttcc
     1981 gaggaagagc gcaagctatt ggataagatt aaagaagcat tgggtcaagg agaagattag
     2041 tgaaagataa agcatatgat attacgatta ttggtggtgg tccaataggt ttatttgcag
     2101 ccttttatgc aggtcttagg ggcgtaacag taaagattat tgagagtttg tcagaacttg
     2161 gtggacagcc tgccattctt tatcctgaga aaatgattta tgatattcca gcttatccat
     2221 ctttaacagg tgttgagttg acagaaaatt taataaaaca attaaaccgt tttgaagatc
     2281 gtataaccat ttgtttaaaa gaagaagtat taacctttga caaggttaaa ggtggttttt
     2341 cgattaggac taataaggca gagcattttt caaaagccat tatcattgct tgtggaaatg
     2401 gcgcttttgc accaagaact ttagggttag aaagtgagga aaactttgct gatcataacc
     2461 ttttttataa tgttcaccag ttggaccagt ttgcgggaca aaaagttgtg atttgtggtg
     2521 gtggagattc agcagttgat tgggctttgg ctttagaaga tattgctgag agtgtgacgg
     2581 ttgtgcaccg tcgtgatgct ttcagagcac atgaacacag tgtggaatta ttgacaacat
     2641 caacggtaaa cctcttaaca ccttatgtgc caaaagcatt gaaaggaatt ggaaacctgg
     2701 ctgaaaaatt agttattcaa aaagtgaaag aagatgaagt gttagaactt gaattggaca
     2761 gtcttattgt tagttttggt ttttcaactt ctaataagaa tttgaaaaat tggaatctcg
     2821 actataagcg ttcaagtatt actgtttcac cactttttca aacaagtcaa gaaggtattt
     2881 ttgccattgg tgatgcagcc gcttataatg ggaaagtgga tttgattgct actggttttg
     2941 gcgaagcacc aacagcagtt aatcaggcca ttaactatat ttatccagat cgagataacc
     3001 gtgtggtgca ttcaacctct ttaattgact aagggagtcg gaagtttcaa gagcagtagc
     3061 aacagctaat attcagtttg tcaggagagg acaaatggtt ttttacctaa gtcttcgtga
     3121 caagttttat actttgtgat aaaatagtca ttgtgaaaaa agtgtgatgg ttttcacaga
     3181 ttaacacatt acaatgaaaa ggggtctatc atgacaacaa cagataaaga gacatttagt
     3241 tcttttatga ataaggtctt ggctggtaca gcgattgcta tcgttgtggc actcattcca
     3301 aatgctattt tagcgacttt cttaaaaccg cttttgccaa acatggcagc tgctgaattt
     3361 ttacacattg tgcaagtctt ccaattcttc acgccgatta tggctggttt cttgattggg
     3421 caacaattta agtttaatcc tatgcaacag ttggctgttg gtggagctgc ttatatcggt
     3481 tctggggctt gggcttatac agaagtcatt caaaaaggtg tggcgacagg aacattccaa
     3541 cttagaggta ttggggactt aatcaatatg atgattacag ctagccttgc ggttttagct
     3601 gtgaaatatt ttgggaataa atttggctct ttaaccatta tcttgttacc tatcactata
     3661 ggaactggcg taggttatat tggttggaaa tttttgcctt atgtgtctta cgtgacaacc
     3721 ctgatcggac aaggtattaa ttcttttaca accttacaac ctattttaat gtctatttta
     3781 attgcagttg ctttctcgct tatcattgtt agtccgattt caactgttgc aatcggttta
     3841 gctattgggc ttaatggaat ggctgcggga gcagcttcta tgggaattgc atcgacagca
     3901 gcagtcttag tgtgggcgac gcttaaagtt aataaatctg gtgtaccaat tgctatcgca
     3961 cttggggcta tgaaaatgat gatgccaaac ttcttgaaac atcctatcat ggctattccg
     4021 atggtgttca ctgcagcaat tagctcatta acagtaccat tgtttaatct tgttggaaca
     4081 ccagcttcat ctggttttgg cttagttggt gcagtaggtc cgattgcttc tttagcaggt
     4141 ggtagctcaa tacttattat tatccttgct tggatcattg ttccgtttgc ggttgctttt
     4201 gcggcgcata aggtttctaa agatattcta aaactttata aagaagacat tttcgtcttt
     4261 gaaggctaat aggaggaaaa tatgttagtt tatattgctg gctcaggagc catggggtgc
     4321 cgttttggtt accaaatttc aaaaacaaat aacgatgtga ttttgttaga taactgggaa
     4381 gatcatatca atgccattaa agaaaacggt ttagtggtga caggggatgt ggaagaaaca
     4441 gttaagcttc ctattatgaa accgactgaa gccactcaag aagccgattt gattatcttg
     4501 tttacaaaag caatgcagtt gccacaaatg cttcaagaca ttaaaggcat tattggaaaa
     4561 gaaaccaaag tgctttgcct tttaaatggt cttggacacg aagacgttat tcgtcagtac
     4621 attccagagc acaacatttt gatgggggtc actgtctgga ctgctggtct agaaggacca
     4681 ggtcgtgctc atcttcaagg agttggtgct cttaacttac aaagtatgga tccaaacaat
     4741 caagatgcgg gacatcaagt agctgacttg ttaaataaag ctaacttaaa tgcgacttat
     4801 gatgagaatg ttgtgccaaa catctggcga aaagcttgcg tgaatggtac catgaattcg
     4861 acttgtgctc ttttagattg tacgattggc gaattatttg ccagtgaaga tggtctcaaa
     4921 atggtaaaag aaatcatcca tgagtttgtt atcgttggtc aagcagaagg tgtcgaacta
     4981 aacgaagaag aaatcacaca atatgtaatg gatacatctg ttaaagctgc tcaccattac
     5041 ccatcaatgc accaagacct tgtgcaaaat caccgattga ctgaaattga ttttatcaat
     5101 ggtgcagtca ataccaaagg tgaaaagctt ggtatcaata ctccatactg ccgtatgatt
     5161 acagaactag ttcacgctaa agaagcagtg ttgaacatcc aataaacgct atcaaatcgt
     5221 tatactcaat attatggcta aagctttaat attgagtttt ttgttatgct gatagtattt
     5281 ttcttaggcg tcattatttg tgagcctaga ttatgagtta ataaaagaaa taataattgg
     5341 tagaatttga tagattttga aaggttatg
//
LOCUS       DATXQK010000070         5233 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.070, whole genome shotgun sequence.
ACCESSION   DATXQK010000070 DATXQK010000000
VERSION     DATXQK010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5233
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.070"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            57..770
                     /locus_tag="VC394_001584"
     CDS             57..770
                     /locus_tag="VC394_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEP5995039.1"
                     /translation="MAKITEENYVSLEDLMQLLNSSESTIRRDLGELEQEGRLHRVHG
                     GAELFHSLQEELSNQEKSVKNSHIKKTIAQRASQFIYDNDVIFIDAGTTTEFLLPFLQ
                     AKNLTVVTNSIHHAARLVELSIETIIVGGYVKQTTDASIGNVALEQIRQMNFDKAFLG
                     MNGIDDSYLTTPDMEEAVIKKAVLSNAKLAYILVDGTKIGQVSFVKVAPINDVTIITL
                     GGSASILKQIKEKAKVIEL"
     gene            767..1678
                     /gene="pfkB"
                     /locus_tag="VC394_001585"
     CDS             767..1678
                     /gene="pfkB"
                     /locus_tag="VC394_001585"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEP5995040.1"
                     /translation="MIYTVTLNPSIDFIVRIDQINLGSVNRMASDDKFAGGKGINVSR
                     ILQRLDIASTATGFLGGFTGRFIEESLSAEGVKTDFVKGDQDTRINVKIKSQEETEIN
                     GQGPVISREQLEDLKTKLSQLTAEDTVVFAGSAPANLGNAVYKELFPLVRQSGAQVVC
                     DFEGQTLIDALTYNPLLVKPNNHELEAIFGTILTSLDDVETYARRLLEMGAQNVIISM
                     AGDGALLVTKEATYFAKPIKGEVKNSVGAGDSMVAGFTGEFMKSQNPIEALKWGVACG
                     TATAFSDDLATIAFIKETYHKVEVEKR"
     gene            1675..3621
                     /locus_tag="VC394_001586"
     CDS             1675..3621
                     /locus_tag="VC394_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEP5995041.1"
                     /translation="MKIQDLLRKDIMILDLQAISKEVAIDEMITKLVEKDIVHDFDVF
                     KKSIMTREEQTSTGLGDGIAMPHSKNIVVDKPAVLFAKSNKGVDYKALDGQPTDLFFM
                     IAAPQGANDTHLAALAELSQYLLKDGFADKLRAAATPEAVIAVFDEASTAKEEVVAPT
                     SGQDFIVAVTACPTGIAHTYMAEEALKKQAAEMGVAIKVETNGASGVANRLTAEDIQR
                     AKGVIVAADKAVEMDRFDGKQFIARPVADGIKKSQELISLILNNEGNTYHAKNGKSET
                     AVSTEKTSLGGAFYKHLMGGVSQMLPFVIGGGIMIALAFLLDNMLGVPNDQLGSLGSY
                     HEIAAIFMNIGGAAFSFMLPVLAGYIAYSIAEKPGLVAGFVAGAIASNGLAFGKVPFA
                     AGGEVSLGLTGVPSGFLGALVGGFLAGGVILALRKLLAGLPRSLEGVKSILLYPLLGV
                     LVTGFLMLFVNIPMAAINTALNDFLQGLSGSSAVLMGLLVGGMMAVDMGGPVNKAAYV
                     FGTGTLAATVANGGSVVMAAVMAGGMVPPLAVFVATLLFKDKFTKEERESGLTNIVMG
                     LSFITEGAIPFGAADPARAIPSFIAGSALTGALVGLAGIKLMAPHGGIFVIALTSNPI
                     LYLVFVVIGALVSGILFGALRKKA"
     gene            3714..4313
                     /locus_tag="VC394_001587"
     CDS             3714..4313
                     /locus_tag="VC394_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEP5995042.1"
                     /translation="MKVKKRRRRAKSSVNRLVLGLVLLNLIVSMWTLKLGNQRLAPYA
                     DHETLTFVRKISHAAQSVAQKKQLYSSVMMAQAILESNNGKSQLSQKPYYNFFGIKGS
                     YKERSVIFPTLEDDGQGNLYQIDAAFRSYGSLTACFLDYARVLNDPLYDKTHKKFWSH
                     YQDATATLTGTYATDTTYNTKLNELIEWYQLTNFDGLMK"
     gene            4465..5172
                     /locus_tag="VC394_001588"
     CDS             4465..5172
                     /locus_tag="VC394_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEP5995043.1"
                     /translation="MTKKKGKLVLISLFVLAACLGAYSAMRQSHKTSNVSAETIASSS
                     TRHFIDEIGPTASTIGQERDLYASVMIAQAILESSNGKSSLSQAPYYNFFGIKGAYNG
                     SSVTMSTWEDDGNGNTYTIDQAFRAYPSIADSLNDYADLLSSSTYIGARKSNTLSYQD
                     ATAALTGLYATDTSYNLKLNNIIATYGLTAYDVANSSAQETGLATSGYVWNEYRRNYT
                     DAETLAVDEAWAKRMTY"
BASE COUNT         1581 a          886 c         1139 g         1627 t
ORIGIN      
        1 aaacgatttc agttaggggt ggttctatct taaaatcaaa acgaaaacaa ttaatcatgg
       61 caaagatcac tgaagaaaac tatgtttctt tagaggattt aatgcaatta ctcaactcct
      121 ctgagtctac tatccgtaga gatctaggag agttggagca agaaggacgg ctacatcgtg
      181 ttcatggagg tgctgaactt tttcactctt tacaagagga actttctaac caagaaaagt
      241 ctgtcaaaaa cagtcatatt aaaaaaacta ttgcgcaaag agcctctcag tttatctatg
      301 acaacgatgt gatctttatt gatgctggga caacgactga atttttatta ccttttttac
      361 aagctaaaaa tttaactgta gttactaatt ctattcatca tgctgctaga ttagttgaac
      421 tctctattga gacgattatt gtaggtggtt atgtcaaaca aacgaccgat gccagtattg
      481 gtaatgtagc tttggaacag attaggcaga tgaattttga caaggcattt cttggaatga
      541 atggtattga tgattcttac ctgacgactc ctgatatgga agaagcagtt attaaaaaag
      601 cggttctgtc aaatgcaaaa ttagcttaca ttttagtaga tggcactaaa attggtcagg
      661 tatcgtttgt aaaagtagct cctattaatg atgtgactat tattacatta ggaggatcag
      721 caagtattct taaacaaata aaggaaaagg caaaggtgat tgaattatga tttataccgt
      781 gaccttaaac ccttctattg actttattgt cagaattgac caaataaatc ttgggtcagt
      841 gaatcgcatg gcgagtgatg ataagtttgc tggaggtaaa ggcattaatg tgagtcgcat
      901 cttgcaacgt ttagacattg ctagcacggc gacaggattc ttgggtggtt ttacaggacg
      961 ttttatcgaa gaaagtttga gtgcagaagg cgttaaaact gattttgtaa aaggtgatca
     1021 agatactcga atcaatgtta aaatcaagtc acaagaagag actgaaataa atggtcaagg
     1081 acctgtcatt agtcgggaac aactagagga tttgaaaact aagctttccc agctaacagc
     1141 agaggataca gttgtctttg caggttcagc gccagctaat cttggcaatg ctgtttataa
     1201 ggaattattt cctttagtta ggcaaagcgg tgctcaggtg gtttgtgatt ttgaaggaca
     1261 gactttaata gatgcgctta catataatcc tctgttggtt aaacctaata atcatgaact
     1321 agaagctatt tttggtacga ttctgactag tcttgatgat gttgaaacct atgcccgtcg
     1381 tcttttagag atgggtgctc aaaacgtcat catttcaatg gctggtgatg gtgctttgct
     1441 ggttactaaa gaagcgactt attttgctaa accgattaag ggagaagtta aaaattctgt
     1501 cggtgctggt gattcgatgg ttgctggctt tacaggcgaa tttatgaaga gtcaaaatcc
     1561 aatagaagcc ttaaaatggg gcgtggcttg tggtacagcg acggctttct ctgatgactt
     1621 ggcgacaatt gcatttatta aggaaacata tcataaagtt gaggtagaaa aacgatgaaa
     1681 attcaagatt tattgagaaa agatattatg attctcgatt tacaggctat ttctaaagaa
     1741 gttgccattg acgagatgat tactaaatta gttgaaaaag atattgtaca tgattttgat
     1801 gtctttaaaa agagtatcat gacacgtgaa gaacaaacat caacagggct tggtgatggg
     1861 attgccatgc ctcattctaa aaatattgta gtagataaac cagcggtctt gtttgcgaaa
     1921 tcaaataaag gtgtggatta taaggcttta gatggtcaac caacggatct cttctttatg
     1981 attgctgcgc ctcaaggagc aaatgatact cacttggctg cgctagcaga attatcacaa
     2041 taccttttga aagacggctt tgctgataaa cttcgtgctg cagcgacacc agaagcagtc
     2101 attgctgttt ttgatgaagc gtcaacagct aaagaagaag tggttgctcc aacaagtggt
     2161 caagacttta tcgttgctgt tacagcttgt ccgacaggaa ttgctcacac ttatatggca
     2221 gaagaggctt tgaaaaaaca agctgcagaa atgggagtag ctattaaagt tgaaacaaac
     2281 ggtgcttctg gtgtggctaa tcgtttaacc gctgaagata ttcagagagc taaaggagtt
     2341 attgttgcag ctgataaagc ggttgaaatg gaccgttttg atggtaaaca attcattgct
     2401 cgccctgttg cagatggtat caagaaaagt caggaattga tttctttgat tttaaacaat
     2461 gaaggaaaca cttatcatgc taaaaatgga aaatctgaaa cagcagtatc aactgagaaa
     2521 acaagtttag gcggtgcttt ctataaacac ttgatgggcg gtgtctcaca aatgttgcca
     2581 tttgttattg gtggcgggat tatgattgct ctagcatttt tattggataa catgcttggt
     2641 gtgccaaatg atcagcttgg aagtcttgga tcctatcatg aaatagcagc tatttttatg
     2701 aacattggtg gagcagcctt ttccttcatg ttaccagtac tagcaggtta tatcgcttat
     2761 tctattgctg aaaaaccggg cttagtagct ggttttgtgg caggtgccat tgcctcaaat
     2821 ggtcttgctt ttggtaaggt accatttgca gcaggaggag aagtgagctt gggattaacg
     2881 ggcgtgccat caggtttcct tggggcgctt gtgggtggtt tccttgccgg tggtgttatt
     2941 cttgcccttc gtaaattgtt ggcaggccta ccacgttctc tagaaggggt taaatctatc
     3001 ctcctttacc cattacttgg cgtccttgtc actggtttct tgatgctttt tgtcaatatt
     3061 ccaatggcag ctattaacac agctcttaat gatttcttac aaggtctttc aggaagctct
     3121 gctgtcctta tgggacttct tgttggtgga atgatggctg ttgacatggg tggtcctgtt
     3181 aataaggcag cttatgtttt tggtacagga actttagcag ctactgtggc aaatggtggt
     3241 tcggtcgtta tggccgctgt tatggcaggt ggtatggtac ctcctcttgc tgtttttgta
     3301 gccactctct tgtttaaaga taaatttacc aaagaagaac gcgaatcagg tttgaccaac
     3361 attgtcatgg gactttcttt catcactgaa ggagcaatcc catttggcgc agctgaccca
     3421 gcgcgtgcaa ttcctagctt tattgctggt tctgccttga caggagctct tgttggtttg
     3481 gctggcatta aattaatggc gccacatggt ggtatcttcg taatcgcttt aacaagcaat
     3541 ccaatcttat atcttgtctt tgttgtgatt ggtgcccttg tatcaggtat cttatttggc
     3601 gctcttcgca aaaaagccta aaaggtaaag aacttttttc ttactaaaac tagaatctgt
     3661 caaggttcta gttttttgct atactagagt taaaggataa gaaatgagga cctttgaagg
     3721 tgaaaaaaag gagaagaagg gctaaatcat cagtaaatcg acttgtctta gggttagtat
     3781 tacttaatct gattgttagt atgtggacac taaaattggg aaaccagcga ttagctccgt
     3841 atgcggatca cgaaactctg acatttgtca ggaagattag tcatgctgct caatcagtcg
     3901 ctcaaaagaa acagttatac agttcggtga tgatggctca ggccatttta gaatccaata
     3961 atggcaagtc acaactaagt caaaaacctt attataattt tttcgggatt aaggggagct
     4021 ataaagaacg gtcagtcatt tttccaactt tagaagacga tggacaaggg aatctttatc
     4081 aaattgatgc tgcttttcgt tcctatggga gcctgacagc ttgtttttta gattacgcaa
     4141 gagttttgaa tgatccactt tatgacaaaa cccacaaaaa gttttggtct cattatcaag
     4201 atgctactgc aaccttaaca ggcacttacg ccactgatac aacttacaat accaaattaa
     4261 atgaattgat tgaatggtat caactcacta attttgatgg tctaatgaaa tagttatcca
     4321 caattttaaa aaaaatcaga agaaatatgt cgatttcaag acaaaagtta tcaatctcat
     4381 taaattatct gtggaaaagt taatttaaac gttcgtgtgc tataatagaa acatagaaaa
     4441 tgtataaaaa aggatatgtg aataatgaca aaaaagaaag gtaagcttgt acttatcagt
     4501 ctttttgttc tagcagcttg tttaggagct tatagtgcaa tgagacaatc tcataaaact
     4561 tcaaacgtgt cagctgagac tatcgccagt tcttcaaccc gacattttat tgatgaaatt
     4621 ggtccaactg ctagtactat tggtcaagaa cgtgatcttt atgcgtctgt tatgatagca
     4681 caagcgattt tagaatcaag taatggtaaa tcaagtctaa gtcaagcacc ttattataat
     4741 tttttcggta ttaagggtgc ctataacggt tcttctgtga cgatgtcaac ttgggaagat
     4801 gatggtaatg gcaataccta tacgattgat caagccttta gagcttatcc aagtattgca
     4861 gactccctta atgactatgc agacttgcta agttctagca cttatatagg tgctagaaaa
     4921 tcaaacacgc tatcgtatca agatgctaca gcagctctaa ctggcttata cgctacagac
     4981 accagttata acttaaaact aaataatatt attgcaacct atggtttgac ggcttatgat
     5041 gttgctaata gctcagctca agaaactggt ctagcaactt cgggttatgt ttggaatgag
     5101 tatcgtcgta attacactga tgcggagacc ctagcagttg atgaggcttg ggctaaacgc
     5161 atgacttatt aatgtaacac taacaacaat ctgcttgcac tgaccccaaa aaagctggat
     5221 actctatttt aag
//
LOCUS       DATXQK010000071         5213 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.071, whole genome shotgun sequence.
ACCESSION   DATXQK010000071 DATXQK010000000
VERSION     DATXQK010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5213
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.071"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            16..1002
                     /locus_tag="VC394_001589"
     CDS             16..1002
                     /locus_tag="VC394_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEP5995044.1"
                     /translation="MDMSKKLRNSLARIVVAAFASFAVMAIPTYHHSTVLAKTVSVNQ
                     TYGEYKDYYTVIGESNIDQSAFPKIYKTTERVYKGQGTSEKRVTVSDVVYNPLDGYKR
                     STGAYGVVTKDMIDMSKGYREKWETNPEPSGWFRFYNRADNEEISEKEYDSRRTKSYK
                     VTNNVPVVLTTLKGKKYNSHLFVRSHLFADSLGGKSIRKNAITGTQMQNVGTRKGGMQ
                     YIEKKVLSHITKNPDVYVFYSAIPEYQGTELLARSVLVSALSSDGVINETVRVFNTAD
                     GFNINYEKGGLLTESPVSEIDNIEDSTTDEIENSVDDSEEIVYNDTTTEEEN"
     gene            complement(1112..2320)
                     /locus_tag="VC394_001590"
     CDS             complement(1112..2320)
                     /locus_tag="VC394_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEP5995045.1"
                     /translation="MTFLDKIKQGCLDGWAKYKILPSLTAAQAILESGWGKHAPHNAL
                     FGIKADSSWTGKSFDTKTQEEYQAGVVTDIVDRFRAYDSWTDSIIDHGKFLNDNPRYK
                     AVVGETDYKKACHAIKDAGYATASGYAELLIQIIKENGLQFWDAEVLKSNKEEKMISS
                     QCREVIEFFINLANAGMGVDKDSFAGWQCADVPCYAAKHWFGVDLWGNAIDLLDSAAA
                     VGWEVHRMPTDANPLTGAFFVQSVPYHQFGHTGIVIEDSDGYTMRTVEQNIDGNPDAL
                     YVGAPARFNTRDFTGVIGWFYPPYQGDTVTQPVSTEPQTSDTIVETAKTGTFTLDVAE
                     INIRRWPSLASEVVGIYKQGDTVSFDSEGYANGYYWISYVGGLGMRDYLAIGQTDKDG
                     NIISLWGKLV"
     gene            complement(2436..2663)
                     /locus_tag="VC394_001591"
     CDS             complement(2436..2663)
                     /locus_tag="VC394_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016480475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEP5995046.1"
                     /translation="MINLKLRLQNKVTLMAILGAIFLLAQQLGIKLPSNIADIANTAV
                     TLLVLLGVVADPTTKGLSDSEQALTYHEPKQ"
     gene            complement(2660..2932)
                     /locus_tag="VC394_001592"
     CDS             complement(2660..2932)
                     /locus_tag="VC394_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5995047.1"
                     /translation="MATELIFGLGGFILAIVTTYNIFNAKSIKHATDITLLQSEVEHL
                     KIVTRQNARRLEEHDEQNKTLITMTEQIKNLNREVRELKDIMRGEA"
     gene            complement(2944..3576)
                     /locus_tag="VC394_001593"
     CDS             complement(2944..3576)
                     /locus_tag="VC394_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5995048.1"
                     /translation="MKTLTLSGKPYPIHEGGKVVKTEVRLIGDNGLFIPIELIGDQTA
                     KGADDLIKEGLDAFVREYVTKYAVAESVQKVEELSLAQKEIEQNAEQAKVTAEAAEKQ
                     AKSLELVIAKSQKMANLQAIHLLTSGSKVEPDIYKGLLELIEPAKQGEYQAYDVFTVV
                     DDKHEEQAGEGNLVFVHVNEPFTYDKQTLKELEEEDKVTVIKYADLVKQD"
     gene            complement(3579..4007)
                     /locus_tag="VC394_001594"
     CDS             complement(3579..4007)
                     /locus_tag="VC394_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1617 family protein"
                     /protein_id="HEP5995049.1"
                     /translation="MDLTLKNKDLNTLYSVLDKIKVTNMRANRGRAKLLAKVVDKIKE
                     YAKDEGDLIDLYAQKDKDGKFVIDERKNIKLADPTKIDELNDLLSELGNEDITIKGHE
                     YSKRFIDFLEYLAESEDEFTSDEIIIIDNILEEFEESKGE"
     gene            complement(4019..>5213)
                     /locus_tag="VC394_001595"
     CDS             complement(4019..>5213)
                     /locus_tag="VC394_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hyaluronidase"
                     /protein_id="HEP5995050.1"
                     /translation="SNQGTRTELESQISGLRAVQQTTASQISQEIRDRTGAVSRVQQD
                     LESYQRRLQDAEDNYSSLTHTVRGLQSDVGSPTGKIQSRFTQLQYQIDQRVTRDGVMS
                     IINQSGDSIKLAIQKAGGINAKMSGSEIISAINLNSYGVQIAGKQIALDGNTVVNGTF
                     TTKIAEAIKIRADQIIAGTLDASKARIINLNASSIVGLDANFIKAKIGYAIVDMLEGK
                     VIKARNSAMLIDLSSAKMDFNSNATINFNSRDNALVRKDGTHTAFVHFSNATPKGYTG
                     SALYASIGITSSGDGVNSASSGRFAGLRSFRYATGYNHTAAVDQTELYGDNVLIADDF
                     SINRGFKFRPDKMEKVLDMNDLYAAVVALGRCWGHLANVGWNTAHSNFTNAVTRELSN
                     YIGII"
BASE COUNT         1453 a         1149 c          995 g         1616 t
ORIGIN      
        1 taattaaagg aggacatgga tatgtctaaa aagcttagaa actctctcgc cagaatagtt
       61 gttgcagcat tcgcatcatt tgctgtgatg gcgattccaa cttatcatca cagtacagtc
      121 ttagctaaaa cagtctcagt aaatcagact tatggtgaat ataaagacta ttatactgta
      181 attggtgagt caaacattga ccaatctgct tttcctaaga tatataaaac aaccgagaga
      241 gtctataaag gacaaggtac aagcgaaaaa agagtaaccg tatctgatgt agtttataac
      301 ccattagatg gctataaacg ttcaacaggc gcttacggcg ttgtcacaaa agatatgatt
      361 gacatgtcta aaggataccg tgaaaaatgg gaaacaaatc ccgaaccaag cggttggttc
      421 agattttata atagagccga taatgaagaa attagcgaga aagaatatga ttcaagacgt
      481 actaagtcat acaaagttac caacaatgta cctgtagtgt taacaacatt aaaaggtaaa
      541 aaatacaaca gtcacttatt tgtacgtagc catctctttg ccgatagcct tggaggaaag
      601 tccatacgga aaaatgctat tactggaact caaatgcaaa atgtggggac acgtaaaggt
      661 gggatgcaat atattgagaa aaaagtttta agccatatca ctaagaatcc tgatgtttat
      721 gtattctaca gtgccatccc tgaatatcag gggactgagt tattagcaag atcagtttta
      781 gtatctgctt tatcttccga tggagtaatt aatgaaactg ttcgtgtttt caacaccgct
      841 gatggattta atattaatta tgaaaagggc ggactcttaa cagaaagccc tgtaagtgaa
      901 attgataata ttgaagattc tacaacagac gaaatagaaa attctgtgga tgattcagaa
      961 gaaatagttt ataacgatac tactaccgaa gaagaaaact aagatttcaa aaaattttta
     1021 gacacacaaa aaaccgccca gaaattaatc tgagcggttt tgtcttatct tggagcttta
     1081 cctccttttt tgctaccggc atatatgtcg gttacactaa tttaccccaa agactgataa
     1141 tgttcccgtc tttatcagtc tgtccaatag ctaggtagtc acgcatacct aagcctccaa
     1201 catagctaat ccaataatag ccattagcgt agccctcgct atcaaagctg acagtatcac
     1261 cttgcttgta gatacctaca acctcgctgg ctagacttgg ccagcgtctg atattgatct
     1321 ctgcaacatc aagggtaaag gtgcctgttt ttgctgtctc tacgatagtg tcagaagttt
     1381 gcggctcggt gctgactggt tgcgtgactg tatccccttg atatggtggg taaaaccaac
     1441 ctatcacgcc agtaaagtca cgagtgttaa aacgagctgg tgcaccgaca tacaaagcat
     1501 caggattgcc atcaatgttt tgctcgacag tgcgcatggt gtaaccgtca ctatcctcga
     1561 taacaattcc cgtatgtcca aattggtgat acggcactga ttggacaaaa aatgctcccg
     1621 tcagtggatt tgcatctgtc ggcatgcggt gaacttccca acctacggca gcagcgctat
     1681 ctagtaagtc aatcgcattt ccccaaagat caacaccaaa ccagtgcttt gctgcataac
     1741 aaggcacatc tgcacattgc cagcccgcaa aactatcttt gtcaacaccc ataccagcat
     1801 tggccaaatt aataaaaaat tcgataactt ctcgacattg agaactaatc attttctcct
     1861 ccttattact ttttaagact tcggcatccc aaaactgcaa gccattctcc ttgataattt
     1921 ggataagtag ctccgcatag ccacttgctg tggcataccc tgcatcttta atagcatggc
     1981 aagccttttt atagtcagtc tcaccaacga ctgccttata ccgtggatta tcgtttaaaa
     2041 atttaccgtg atcaataatg ctgtcagtcc aactatcata cgccctaaat cggtccacaa
     2101 tatccgtgac aacacctgct tgatattcct cttgggtttt ggtatcaaat gatttaccag
     2161 tccaagagct atctgcctta ataccaaaca gagcgttgtg tggggcatgt ttgccccacc
     2221 cgctctctaa gatagcttgt gctgcggtca aggatggcaa gattttgtac ttagcccagc
     2281 catctaaaca gccttgttta attttatcta aaaaggtcat ctgtcctcct tatctaaaaa
     2341 tggataaaag atcagagcaa caacagataa tggcacatac agtattgcta ttgctataac
     2401 taatgctaat cgtgtgattg ctcgcatgtc ccctcctatt gttttggttc gtggtaagtc
     2461 aaagcttgct cactgtctga caggcctttc gtggttggat ctgcgacaac accgagcaat
     2521 accaaaagcg ttacagctgt gtttgcaata tccgcgatgt ttgatggtag tttaatacct
     2581 aattgctgtg ccagtaaaaa gatagctcct aaaatagcca tcaaggttac tttgttttgt
     2641 agtcgtaatt ttaaattgat catgcttcac ctctcataat atctttaagt tctcttacct
     2701 cacgattgag gtttttaatt tgctctgtca ttgtgatgag cgttttgttt tgctcatcat
     2761 gctcctcaag acgccgagca ttttggcgtg taacaatttt taaatgctct acttcagact
     2821 gcaacaaagt aatatctgtc gcatgcttga tggattttgc attaaaaata ttgtaagtcg
     2881 tgacgatagc taaaataaag ccaccaagac caaatatcaa ctctgttgcc atagcttacc
     2941 tcgctaatcc tgcttaacta agtccgcata cttaatgact gtgactttat cctcttcctc
     3001 tagctcttta agcgtctgtt tgtcataagt aaacggctcg ttgacatgta caaagacaag
     3061 attgccttcg cccgcttgct cttcgtgttt atcgtcgacc accgtaaaga catcataggc
     3121 ctgatactcg ccttgtttgg ctggctcgat tagctctaaa agacctttat agatatcagg
     3181 ttcaactttg cttccgcttg ttagtagatg gattgcttgt agattagcca ttttttgaga
     3241 ttttgcaatg acaagctcca gagatttagc ttgtttttca gcggcttctg ctgttacctt
     3301 tgcttgctcc gcattttgct caatctcttt ttgtgcgagg ctcaactctt ccactttttg
     3361 cactgattct gctacggcgt atttagtcac atactcgcgt acaaaagcat ctagtccctc
     3421 tttaataagg tcatctgctc ccttagctgt ctgatcaccg attaattcaa tggggataaa
     3481 tagcccattg tcaccgatta agcgaacctc tgtttttaca actttaccgc cttcatgaat
     3541 tggataaggt ttgcctgata gtgttaatgt cttcatagtt attctccttt gctttcttca
     3601 aattcctcta aaatgttatc gatgataatg atttcgtctg aggtaaactc atcttccgat
     3661 tctgccaagt actccaaaaa gtcgataaaa cgcttagaat actcatggcc tttgatagta
     3721 atgtcctcat taccaagttc ggacaataag tcgttgagct catcaatctt agtcgggtct
     3781 gctagcttga tgtttttgcg ctcatcgatg acaaacttgc catctttgtc tttttgagca
     3841 tacagatcaa taaggtcacc ctcatccttg gcgtactctt tgattttatc gactactttt
     3901 gcgagtagct tagcacgtcc gcggtttgct cgcatgttcg tgaccttgat tttgtctagt
     3961 acactatata atgtgtttaa atctttgttt ttaagcgtta aatccatgtt ttctcctttt
     4021 aaataatacc aatgtaatta ctcaactctc tagtgaccgc atttgtaaaa ttgctatgag
     4081 cagtattcca gccgacgtta gccaagtgcc cccaacagcg gcctaaggct actacagccg
     4141 catacaagtc gttcatgtcg agcacttttt ccattttgtc tggtctaaat ttaaatcctc
     4201 gattgatgct aaagtcatct gcaatcaaga cattatcacc gtatagctcg gtttggtcga
     4261 ctgcagcagt atgattatat cccgtagcat acctaaatga ccttagccct gcaaaacgtc
     4321 cagatgacgc actgttgacc ccatcaccag atgaggtgat accaatagag gcatataatg
     4381 ccgaacctgt ataacctttt ggtgtggcat tactaaagtg tacaaaggca gtgtgggtac
     4441 cgtctttacg cactaaagcg ttatctcggc tgttaaagtt gatggtcgca ttactattaa
     4501 aatccatctt agccgagcta agatcaataa gcatagcgct attgcgtgcc ttaatcactt
     4561 taccctcaag catgtcaaca atcgcatagc caattttagc tttgataaag ttagcgtcta
     4621 aaccaacgat actgctggcg tttagattaa taatacgagc tttagaggca tctaaagtgc
     4681 ctgctatgat ttggtcagct ctgattttga tagcctcggc tatttttgtg gtaaaggtgc
     4741 cgttaactac cgtgttacca tctaaagcta tctgcttacc agcgatttgc acaccgtagg
     4801 agtttaagtt aatcgctgag atgatttcgc tgccagacat cttggcatta atgccgccag
     4861 ccttttggat agctaattta atgctgtctc cagactggtt aatgattgac ataactccgt
     4921 cacgagtcac tcgctggtct atttggtatt gcagctgcgt aaagcgtgat tggattttac
     4981 cagtcgggga tcccacatca ctctgcaaac ctctaactgt atgggttaag ctactgtaat
     5041 tatcttccgc atcctgcaaa cgacgctgat aactctctag gtcttgctgc acacgactga
     5101 ctgctcctgt cctgtctcta atctcttgtg agatttggct agcggttgtc tgttgtaccg
     5161 ctcttagtcc gctaatttgt gactcgagct ctgtcctcgt gccttgattt gag
//
LOCUS       DATXQK010000072         5182 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.072, whole genome shotgun sequence.
ACCESSION   DATXQK010000072 DATXQK010000000
VERSION     DATXQK010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5182
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.072"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            16..939
                     /locus_tag="VC394_001596"
     CDS             16..939
                     /locus_tag="VC394_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEP5995051.1"
                     /translation="MKFFEENYSQEIPTRIKNLRKKHNITQSELGNAGQVSQVESGKR
                     PITSSMLVYLNALTASSYTYIVFGELDEFIENLFHYFFSSILYRDLEAVDENLYSFMS
                     DDLISIQSSCLSIAKTFANFNIQRKRFMISTETEMDTFHKKDDIDVWVGGKSYNPARS
                     FRNNPINELTVIDFEEMADILLLMLRDNLIRSFEINVCNTLFELDKNGEPTTFNLDKI
                     DSIINKWWSENVSTEIIPNLIKKLRENPLFNIGFMVNDILERMYKEDIPKSYLTSVPL
                     VISKKARSTLSIKSNGSQKIDKSKFAQILSL"
     gene            1005..1757
                     /locus_tag="VC394_001597"
     CDS             1005..1757
                     /locus_tag="VC394_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEP5995052.1"
                     /translation="MAIPDLDKFSSNLQQGKIPVIKHFELNLDKGSHWAIAGNSGSGK
                     PYALTYFLSVLKPMSGLIIIDPKFDTPSQWARENKIAVIHPVENHSKSDFVSQVNEQL
                     NQCATLIQKRQAILYDNPNHQFTHLTIVIDEVLALSEGVNKNIKEAFFSLLSQIALLG
                     HATKIHLFLGSQRFDHNTIPISVREQLNVLLQIGNINQKTTQFLFPDLDPEGIVIPTG
                     HGTGIIQVVDNEHSYQVLPLLCPTYYTKRGIL"
     gene            1754..2356
                     /locus_tag="VC394_001598"
     CDS             1754..2356
                     /locus_tag="VC394_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5995053.1"
                     /translation="MTTKTYFMRSFNLILYLLLIAPICYVFGWLFNLVSVQLSTVTFV
                     NLDTVKNVTDTISSVCYGFALISLLLALLSGLKMIQRLNTDSLGNYCQSIYRTFLLRH
                     FLFQRERVQKVSNFEHQTVTTINPIHNGFNRAVRKCIVDIRKDAITVFLKVPRDQQGQ
                     KILKDTGVQLKEEIVIQHTDYYFSSPVRVRNQLWFIGQKR"
     gene            complement(2832..3113)
                     /locus_tag="VC394_001599"
     CDS             complement(2832..3113)
                     /locus_tag="VC394_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEP5995054.1"
                     /translation="MLSKSLQVPLEQLIAEDIPKMTLIIKDEEIKQCDRNTHIMLFGT
                     LLIAISIYPIMSWLGWWGYGPVAILWLIIMRIALRIERFKKTYDVQTYR"
     gene            complement(3120..3164)
                     /locus_tag="VC394_001600"
                     /pseudo
     CDS             complement(3120..3164)
                     /locus_tag="VC394_001600"
                     /inference="COORDINATES: protein motif:HMM:NF013540.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3567..4409
                     /locus_tag="VC394_001601"
     CDS             3567..4409
                     /locus_tag="VC394_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEP5995055.1"
                     /translation="MVSFISRVFKGMIIALGFILPGVSGGVLAAILGIYERMISFLAH
                     MRDNFIENVLFFLPVGIGGILGIALFSFPVEFLLKHYQVSVLWGFAGAIVGTIPSLIK
                     ESTKQSQRDKADWLWLVLTFVISGLGLYFLNDLIGTLPANFLTFILAGALIALGVLVP
                     GLSPSNLLLILGLYGPMLIGFKSLDLLGTFLPIAIGGVLAILAFSKSMDYALQHHHSK
                     VYHFIIGIVLSSTLLILIPNSSSPESISYSHAGILTWLMAFVLFALGIWLGLWMSQLE
                     EKYK"
     gene            complement(4456..5097)
                     /locus_tag="VC394_001602"
     CDS             complement(4456..5097)
                     /locus_tag="VC394_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEP5995056.1"
                     /translation="MKDLLKQYQAKPLGEEKRYAVFLPLILVNDDWHVLYEVRSQHIS
                     QPGEVSFPGGQVENQETLQEAAIRETVEELTVDASQIQLWGEIDYLVQSSRTIHCFVG
                     QLVVDDWKSIQPNEEVDKVFIVPLRQLLFTDPVYYRLEATPIETTDFPFDRIRNGKYY
                     QFSQEYRSIPFYENLEETIWGMTAQFTKCLTDILNDESIAKKNPSDIENSKGR"
BASE COUNT         1615 a          971 c          865 g         1731 t
ORIGIN      
        1 atagggggat tatatatgaa attttttgaa gaaaattatt cacaagaaat acctactcgt
       61 atcaaaaatt taagaaaaaa acacaatatt acacaaagcg aactaggcaa tgcaggtcaa
      121 gttagtcaag ttgaaagtgg taagcgacca attacgagtt cgatgttagt ttatttaaat
      181 gctctaaccg cttcgagcta tacgtatatc gtttttggag agttagatga gtttattgag
      241 aatctgtttc attatttttt cagttctatt ttatatagag atttagaggc tgtagatgaa
      301 aacctatatt ctttcatgag tgatgattta atttcaatcc aatcaagttg tctaagtatt
      361 gccaagacat ttgcaaattt caatattcaa agaaaaagat ttatgatttc tactgaaact
      421 gaaatggata cttttcacaa gaaagatgac attgacgtat gggttggtgg aaaaagttat
      481 aaccccgcta ggagttttag gaataatcca ataaatgaat taactgttat tgattttgaa
      541 gaaatggctg atattctttt gttgatgttg agagataatc taattaggtc gtttgaaatt
      601 aatgtctgca acacactgtt cgaattagat aaaaatggtg aaccgacaac atttaatcta
      661 gataaaattg actcaataat caataagtgg tggtctgaga atgtctcaac agagataata
      721 cccaacctca taaaaaaact tagagaaaat cccttgttta atatcggatt tatggtaaat
      781 gatatattag aacgaatgta taaagaggac ataccaaagt cttaccttac ttctgttcca
      841 ttagttattt ccaaaaaagc aaggtcaact ttatcgatta agtcaaacgg aagtcaaaaa
      901 atagataaat caaaatttgc acagattttg tccctttaga gacggatgac ttacacattc
      961 aacgtggatt attcttccct tggagaaagg ggatttcaga acgcttggcg attcctgatt
     1021 tagataaatt ttcatctaac ttacaacagg ggaaaattcc tgtcataaag cacttcgaac
     1081 tcaatttaga taaggggagt cactgggcta ttgctggaaa ttcaggttct gggaaaccgt
     1141 acgctctcac ttacttcttg agtgtcctga aacctatgtc cggcctaatt atcattgacc
     1201 caaaattcga tacccctagc caatgggcaa gagaaaataa aattgccgtt atccacccag
     1261 tagaaaacca ttcaaaatct gatttcgttt cacaagtcaa tgaacaatta aaccagtgtg
     1321 caactctcat tcagaaacga caagctatct tgtatgataa tcccaatcat caatttaccc
     1381 atctaactat tgttattgat gaagtcctcg ctctatcaga gggagtcaat aagaatatca
     1441 aagaagcttt tttctcccta ctatcacaaa ttgccttgtt gggacatgct actaaaatcc
     1501 atctattctt gggaagccag cgttttgacc ataacactat tcccatttca gtgagggaac
     1561 agctaaacgt attattgcaa attggaaata tcaatcagaa aaccactcaa ttcttatttc
     1621 cagacctaga tccagagggg attgtcattc caacaggaca cggaacaggt atcattcaag
     1681 ttgtcgataa tgaacactcc tatcaagttc tacccttact ctgtccaacc tactatacta
     1741 agcgaggtat cctatgacca ctaaaaccta cttcatgaga agcttcaatc tcatattata
     1801 cttactgctt attgctccaa tctgctatgt gttcggttgg ctgttcaatc ttgtttcagt
     1861 tcaattatct accgtcacat ttgtcaatct ggatacggtt aaaaacgtta ctgataccat
     1921 tagttctgta tgttatggat tcgctcttat ttctttactt ctagccttat tatctggatt
     1981 aaagatgata caacgcttga atacagatag ccttgggaac tactgccaat ctatttatcg
     2041 cacctttcta ttaagacact tccttttcca gcgtgaacga gtacagaagg taagcaactt
     2101 tgaacaccag accgttacca ctatcaatcc aatccataac gggtttaacc gagctgtacg
     2161 taagtgtatc gtagatatac gaaaagatgc cattactgta tttctaaaag ttcctagaga
     2221 ccaacagggg caaaaaatcc taaaggatac gggagtacaa ctgaaagaag agattgtcat
     2281 ccaacacaca gactattatt tctcttctcc tgttcgagtt cgtaaccaat tgtggtttat
     2341 tggtcaaaag cgttaattcc ttggggctgt ggcttgcgtt aagcaaaagc cagacagccc
     2401 cttttttata ttcttatcta tcatgaccta ttggggttac tacgacaacg aaagttagaa
     2461 gctgtgggta tttatttatc ttgaaaagat tgctgaacta acgcaataac tactctacca
     2521 ttcactctac cttttattaa gcaactatca ataaatattg atacaataag gtttataaca
     2581 gctaaaatag aaaatactta acctttacag aagtaaaata attgagatga gaaaagccta
     2641 tgaaatgaac gtttcaaagg cttttttcgt gtttaaaaaa acaaatgaaa aaacaaaata
     2701 gagagtaggg ttattaacac tgtcaatcct gtgtacgcaa taacaatcca tgctttttga
     2761 taaggatatt ttgctttttc tttaatctta tcaatctgat ctaagctctc accctttgct
     2821 ataacttcaa gttatcggta agtctgaaca tcatatgtct ttttaaatcg ctcaattctt
     2881 aaggcgatac gcatgattat caaccataag atagcaaccg gaccatatcc ccaccaacct
     2941 aaccaagaca ttataggata aatactgata gcaattagga gtgtcccaaa taacataata
     3001 tgcgtattcc tatcacattg tttaatctcc tcatccttaa taattaaagt catttttggg
     3061 atatcttctg ctattagttg ctctaaagga acctgtaagg acttacttaa catcaaaaga
     3121 ttttgcaggt ctggatagct tttaccgttt tcccagtttg agatagtctt cttgaaacat
     3181 acatcatttc agcaagctct tcttgtgaca tctgtaattt ttttcaataa taagataact
     3241 ggtttttaaa gcccataata aatctccttt ttaatagcta ctataatcct tcttcaataa
     3301 gtttactatc aaaagtcttt gacctaagct ttttttcgtt tatcttttta ataattatcg
     3361 taaggtttac agtctttgta aattttattt acaactttga caatcaaact tgacttgatc
     3421 acaaagacat ggtgtttaag gaaaatatct tcaaaattga atgtattatt tattaggaac
     3481 aattatttta gtagggatat gcagctttgt gtcagcaatt ctttaaattt atgatacaat
     3541 aaaatcattc ttttcaggag gcaacaatgg tttcatttat ttcaagagtt tttaaaggca
     3601 tgattattgc actaggtttt attttacctg gtgtatccgg tggtgttctc gcagccattt
     3661 taggaatcta tgagcgaatg atttcctttt tagctcatat gagggataac tttattgaaa
     3721 atgtcttatt tttcttacca gtaggaattg gtggtatttt aggtattgct ctcttttctt
     3781 tccccgttga gtttttgctt aagcactatc aagttagcgt cttatgggga tttgctggcg
     3841 ctattgtcgg cactattcct agtctgatta aggagtcaac caagcagtct caaagagaca
     3901 aagctgactg gctatggcta gtccttacct ttgttatttc aggactaggt ctttacttct
     3961 taaatgactt aatcggtacc ttaccagcta actttttaac ctttatttta gcaggtgcct
     4021 taattgcttt aggagtactg gtccctggac taagcccgtc aaatctctta cttatcttag
     4081 gactctatgg tcctatgtta atagggttca aatctttgga tttattaggg actttcttac
     4141 ctattgctat cggaggagtc ctagccatct tggctttttc taaaagtatg gattatgctt
     4201 tacagcacca tcattcaaaa gtttaccact ttatcattgg tattgtatta tccagcacgc
     4261 tattaattct tattccaaat agtagtagtc ccgaatccat ttcctacagt catgcaggta
     4321 tcttaacctg gcttatggcc tttgtgttat ttgctcttgg tatttggctc ggactttgga
     4381 tgagtcaatt agaagaaaaa tacaaataac ctttacagtt tgttgacaaa aagttgacgc
     4441 caacaaacta gtgctctatc tccccttaga attttcaata tctgaggggt ttttctttgc
     4501 aattgattca tcatttagaa tatcggttaa acacttagta aactgggcag tcattcccca
     4561 tatggtttct tctagatttt cataaaacgg aatactacgg tactcttgac taaattggta
     4621 gtatttccca ttacgaatac ggtcaaaagg aaaatccgtt gtctcaatag gagtggcttc
     4681 taaacgataa tagacaggat cagtgaaaag tagctgacgt aaaggtacta taaagacctt
     4741 gtcaacttct tcgttaggct gtatgctttt ccagtcatca acgactaatt gaccaacgaa
     4801 acaatgaata gttctagaag actgtactaa ataatcaatt tcaccccata gctggatctg
     4861 ggacgcatca acagtcaatt cctcaactgt ttctcggata gcagcttctt gaagtgtctc
     4921 ctgattttct acttgtcccc ctgggaaaga aacttccccc ggttgtgaaa tatgctgact
     4981 tctgacttca tataagacat gccagtcatc attgacaaga ataagaggta aaaatacggc
     5041 gtaccgcttc tcctctccta gtggttttgc ctgatactgt tttaataagt ccttcatagc
     5101 cttccctaat ttctctttcc gttaatctta tcttcatcat aaccaatcct agctttttgg
     5161 tcaatttatt actcctagtt ag
//
LOCUS       DATXQK010000073         5161 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.073, whole genome shotgun sequence.
ACCESSION   DATXQK010000073 DATXQK010000000
VERSION     DATXQK010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5161
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.073"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            190..363
                     /locus_tag="VC394_001603"
     CDS             190..363
                     /locus_tag="VC394_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2758 domain-containing protein"
                     /protein_id="HEP5995057.1"
                     /translation="MKIKLFYQKHNESLDDFEYRVNQFTLSVSVIDIKLSEATYGNYE
                     DMSTTTSLLVLYR"
     gene            369..542
                     /locus_tag="VC394_001604"
     CDS             369..542
                     /locus_tag="VC394_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001873750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5995058.1"
                     /translation="MKLKLHTRGGNTITIQGDSAFYDELVKYLLSGRQPNWVTCPSAV
                     INLSDIIAITKEK"
     gene            544..870
                     /locus_tag="VC394_001605"
                     /pseudo
     CDS             544..870
                     /locus_tag="VC394_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            866..1111
                     /locus_tag="VC394_001606"
     CDS             866..1111
                     /locus_tag="VC394_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5995059.1"
                     /translation="MSNQMAVFNQFWAYQTLFQPTAQDIKLAILGYFTGTLWACEVSV
                     LEDVELGYRVIVSYVSCFDKSDRLLKIAQAVENSQLG"
     gene            1125..1283
                     /locus_tag="VC394_001607"
     CDS             1125..1283
                     /locus_tag="VC394_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5995060.1"
                     /translation="MQTEVKDGFIYFKENGRIGVVEIPTFGKLTLSSKEGYIHQVDHS
                     FSTKHNQK"
     gene            1374..2162
                     /locus_tag="VC394_001608"
     CDS             1374..2162
                     /locus_tag="VC394_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5995061.1"
                     /translation="MYKQTNKTYADILEKVEAATAQWIIRKKASSLKDDVIITDEDIQ
                     AEKERIFIEKYGTALFDFKIDSYRKELDDLKIKAREQVNQFNDFVKELEQDDRYTERG
                     KVDLYRAERKKAEKTLKEIGEAQHKLNVDIQKIEVESAQNAWKKLESEMTPDSITPSE
                     YQYLEMMLSRNDSDEMRKKLAEKFHYHLMVLEYLNADRKFGEVIIKHPLEGVKNQSVN
                     YIKIGDVRLPEVYESTYIHELLKHYKNDIYFGESYANHEWDEEQ"
     gene            2159..2425
                     /locus_tag="VC394_001609"
     CDS             2159..2425
                     /locus_tag="VC394_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5995062.1"
                     /translation="MMSLECLRIYLKKSQFTELEHLLFRIIVLGGYPDDMYFPSRVRT
                     IITSLVNNIRKNLDSEGYRSVEELEEAIEKAISEHDEITQKGGG"
     gene            2467..2805
                     /locus_tag="VC394_001610"
     CDS             2467..2805
                     /locus_tag="VC394_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEP5995063.1"
                     /translation="MVAMVTSKTITEAAERAGITRGTAHRYLKDITFKKACRRYRSEL
                     MQQTTSLLQSASIEAVEVLRNIMLDDSISPYARQQSAQTILSTAYKAHELDDIVEMVE
                     ELEARFEDEQ"
     gene            2795..3070
                     /locus_tag="VC394_001611"
     CDS             2795..3070
                     /locus_tag="VC394_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5995064.1"
                     /translation="MNSNLKKRVDKLRRLHSLDNASGIVFVEYADGKYQIKKQDHVPK
                     FIETRLIAEFESEEEAENFLSTIPSGYIIFWDIFPEEVEHVKKNKYL"
     gene            3045..3290
                     /locus_tag="VC394_001612"
     CDS             3045..3290
                     /locus_tag="VC394_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5995065.1"
                     /translation="MLKKINTYRRLEIIKNKLDEASKDSIEDIMLDWFRALHYALKVD
                     DTIDLPTFLEFKDNKALRDKIMADLGDFDPFEDLNIE"
     gene            3571..3918
                     /locus_tag="VC394_001613"
     CDS             3571..3918
                     /locus_tag="VC394_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEP5995066.1"
                     /translation="MNAKEQLKELKPLFALMTLFEEQRDKDIKLMNAFRNPELLNGIE
                     KGTAKQLLYLAKERDKRLAMIATLQDERQIAVIKARYVDDLSWDEIPDKLGYSRNTVF
                     KLHREALEVLDEP"
     gene            3908..4279
                     /locus_tag="VC394_001614"
     CDS             3908..4279
                     /locus_tag="VC394_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEP5995067.1"
                     /translation="MNHKNAIRKLKEFHRWQRIANSLNLTYNECYQFDIEYYSFRRKH
                     LEISRKCALEELDAIKHAINQLSKIEYRKILIECYLIGEKKPQQDIIAELNRSKSWYY
                     ETKRRALLEFVEFYRDGVLKK"
     gene            4432..4659
                     /locus_tag="VC394_001615"
     CDS             4432..4659
                     /locus_tag="VC394_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation repressor RelB"
                     /protein_id="HEP5995068.1"
                     /translation="MAMVSLRLNSAEEELFRSYATHTGKTLSELFKTALAQQIEDQLD
                     YETGIQALKRFEENPITYSIDDLIEELENDL"
     gene            4649..4918
                     /locus_tag="VC394_001616"
     CDS             4649..4918
                     /locus_tag="VC394_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEP5995069.1"
                     /translation="MTYKLVVSDEVKKQLKKMDKHVGLMLAKDMKKRLDGLNNPRQFG
                     KALTGQYKGLWRYRVGNYRVICDIVDNEMIILALEVGHRKEIYKR"
BASE COUNT         1836 a          838 c         1034 g         1453 t
ORIGIN      
        1 attatttttt tagtagatat gttatttctt tacattattt ttattttttc tcccttatct
       61 atatagaaaa gtggcggtag gtggtggttg tgctggcaaa tggctcaacc atgcggtttt
      121 aagcacacca ccactttatt tagggttgtg tctacaccca ccactttgag ccaaaacagg
      181 agaaaaaaca tgaaaatcaa actattttat caaaaacaca atgaatcttt agatgatttt
      241 gaatatcggg tcaatcaatt taccctatca gtatctgtaa tagatatcaa actttcagaa
      301 gcaacttatg gcaattatga agacatgagt accacaactt ctttactggt cttgtacagg
      361 taactgatat gaaactaaaa ttacacacac gaggcggcaa caccatcacc atacaaggag
      421 acagtgcctt ttatgatgag ttggtcaaat accttctttc tggccgacaa ccaaactggg
      481 taacatgtcc ttctgccgtc atcaatttat cagacattat agcaatcaca aaggagaaat
      541 aatatgagaa cattttcaga cacaccaaaa acattcacat ttcactacac gtttaaagac
      601 tttgacaccg cacaagtagc ctgtcacgcc attctaggct atatgactgg tacttatgag
      661 caaccagtga ttgatgccac ctatcacaat gataatcaag gcgggcatgc taatcagtta
      721 gtattagaat atgccgagga cagaaagtta agcaaggtct tcaagcgtat ctgtgacagc
      781 ttcaaggatt attacaacca acctgaggat atgacggatg aagaacttga tgacatggtt
      841 caagaaaatg aattaatcaa ggaagttgag caaccagatg gcagtattta atcaattttg
      901 ggcgtatcag acactttttc aaccaacggc acaagatatt aagttagcta ttttaggata
      961 cttcacaggc acgctatggg cttgtgaggt gtccgtgttg gaagatgttg aacttggcta
     1021 ccgtgttatc gtctcttacg ttagttgttt tgataaaagc gatagactgc tgaaaattgc
     1081 tcaagcagtt gagaatagtc agttaggatg aaaggaggta tttaatgcag acagaagtga
     1141 aagatggctt tatttatttc aaagaaaatg gtagaatagg agtagtagag atacctactt
     1201 ttggtaagct aaccctatct agcaaggaag gctatattca tcaagtagac cacagctttt
     1261 ctactaaaca caatcaaaaa taattatact gaccgaagaa cggaggtata ttcatgaagc
     1321 ttaatggctt ttttggatat acctcttttt gcatttaaga aaggaaagac acgatgtaca
     1381 aacaaactaa caaaacttac gccgatattt tggaaaaagt tgaagccgca acagcgcaat
     1441 ggattattag aaaaaaagct tcaagcctta aagatgacgt gattatcact gatgaggata
     1501 tacaagctga gaaagagcgt attttcattg agaaatatgg gacagcactg tttgatttta
     1561 aaattgatag ctaccgtaaa gaattagatg atttaaaaat caaagctcgt gagcaagtaa
     1621 accaattcaa tgattttgta aaggaactag aacaagacga ccgttacaca gaacgtggta
     1681 aagtggattt ataccgagca gagcgaaaaa aagctgaaaa gacccttaaa gagattggag
     1741 aagctcagca taaacttaat gttgatattc aaaagattga agtagaatcg gctcaaaatg
     1801 cttggaaaaa gttagaatct gaaatgacgc ccgattcaat cacccctagt gaatatcagt
     1861 atttagaaat gatgttatca cgtaatgata gtgatgaaat gcggaaaaaa cttgctgaga
     1921 agttccatta ccatctgatg gtcttggagt atttgaatgc tgaccgtaaa tttggagaag
     1981 ttatcatcaa gcacccatta gaaggggtta aaaatcagtc agttaattat attaaaattg
     2041 gggatgtccg attgcctgag gtttacgaaa gcacttatat tcatgaactt ttgaagcatt
     2101 acaagaatga catttacttt ggcgagagct acgcaaatca tgaatgggat gaagagcaat
     2161 gatgtcattg gaatgtttaa gaatatacct taagaaaagc cagtttacag agcttgaaca
     2221 cttgctattc agaattatag tgttaggggg gtatcctgat gacatgtatt tcccctcaag
     2281 ggttcgtacg atcatcacaa gcttggttaa taatatacgt aagaatttag attctgaggg
     2341 ttaccgtagt gtcgaagaat tggaagaagc tatagaaaag gctatttcag agcatgacga
     2401 aatcacacaa aaaggagggg ggtgattttt atagaacatg gtttaacacc taaacaagag
     2461 aaattcatgg tagcaatggt gaccagtaaa actatcaccg aggcagccga aagagcaggt
     2521 atcacaagag gaactgccca tagatacctt aaagacatta ctttcaaaaa ggcttgtcgc
     2581 cgttatcgta gtgagttaat gcagcaaacc acatccttat tacagagtgc cagcattgaa
     2641 gccgttgagg tgctacgtaa cattatgttg gatgattcta ttagtccata tgcaaggcaa
     2701 caatcagccc aaactatctt aagcactgct tataaagcgc atgaactaga tgatattgtt
     2761 gaaatggttg aggaactaga agcgagattt gaagatgaac agtaacttaa aaaagcgagt
     2821 tgataagtta aggcgactac atagtttaga taacgcttct ggtattgtct ttgtagaata
     2881 cgcagatggc aagtatcaga ttaaaaaaca agatcatgtc cctaaattca ttgagactag
     2941 acttatagca gaatttgaaa gcgaagaaga agcagaaaac tttttaagca ctattccaag
     3001 tggttacatc attttttggg atattttccc cgaagaggtt gaacatgtta aaaaaaataa
     3061 atacttatag aaggctcgaa atcatcaaga ataagctcga tgaagcgagt aaagatagta
     3121 ttgaagatat aatgctggac tggtttaggg ctttgcatta tgctcttaaa gtagatgata
     3181 caattgattt accaacgttc cttgaattta aggacaacaa ggcattgaga gataaaataa
     3241 tggcagacct tggagatttt gacccatttg aagatctaaa tatagaatag ttaaatggtg
     3301 acagcatcgc cacaaggtcg tcataaggag atagtttttt gactgtctcc ctttttcgtc
     3361 atctattccc catcagacta aaataaaaca tggttttagc gcttctgtct gctaagttga
     3421 tgattttata ttataagctc tgaaaagcgc ttagaaacga ttttagagcc gttcttccgc
     3481 tatcaagaat gactaaaaca aaaagacgtc cacacggaac gccttcatga ttatatctgt
     3541 taacacaatt ataccacact gggggctttc atgaacgcca aagaacaact taaagaattg
     3601 aaaccacttt ttgctttaat gaccttattt gaggaacaac gagacaagga catcaagcta
     3661 atgaatgctt ttcgtaatcc tgagttacta aatggcatcg aaaaaggtac agctaaacaa
     3721 cttttatatt tagctaagga acgtgataag agactagcca tgattgccac attgcaagat
     3781 gagagacaga ttgccgttat caaagctaga tacgtggatg acttatcatg ggatgagata
     3841 cctgataaac taggttattc aaggaatacc gtttttaaat tacatagaga agctttagag
     3901 gtattagatg aaccataaga acgctattcg taaactaaaa gagtttcata gatggcaacg
     3961 tatcgctaat agccttaatt taacctataa cgagtgttac cagtttgata tagaatatta
     4021 ttcctttaga cgtaaacacc ttgaaataag ccgaaaatgc gccctagagg agctagatgc
     4081 tattaagcat gccattaatc aactatctaa gatagagtat agaaagatac tgattgagtg
     4141 ttacttgatc ggtgagaaaa aacctcaaca agacatcata gcagaactta acagaagtaa
     4201 aagctggtat tatgagacta agagacgtgc tttacttgaa tttgtggaat tttacagaga
     4261 cggtgtgcta aaaaaataat gttcgtttat gataacagag caatagatat aaatgttaat
     4321 aatacagtgt tagaagagct taacaaggta tatcaatacg ggtaaataat tgactgattc
     4381 ctccttatgt tatacaatgt attacagata aacaaacaga ggtggaagaa tatggcaatg
     4441 gtatcattaa gactgaacag cgcagaggaa gaacttttca gaagctacgc tacacacaca
     4501 ggcaaaacat tatctgaatt attcaagaca gcactagcac aacaaattga agatcaattg
     4561 gactatgaaa caggtattca agctttaaaa cgctttgaag aaaatcccat aacttattct
     4621 atcgatgatt taattgagga gttggaaaat gacttataag ctagtggtga gtgatgaagt
     4681 caagaagcaa ctcaaaaaga tggataaaca tgtagggctt atgttagcca aagacatgaa
     4741 gaaacgactt gacgggttaa ataatcctag acaatttgga aaagccctca caggtcaata
     4801 taaaggactt tggcgctatc gtgttggtaa ttatcgggtg atttgtgaca ttgtggataa
     4861 tgaaatgatt atccttgctt tggaagttgg acacaggaaa gagatataca agcgctagca
     4921 aatacaagac atggtacgag gtataataaa aagcgcagct aatgaccacg ctatttcttg
     4981 cttactgaac tcattatttt aattcctttt tagttaccct ttagatttct gatagtgtat
     5041 ctagtttaca ctgtttttag agaaataaga gtgataactc acttggttta cagctacttt
     5101 acactactaa atatgagtag tataacaaaa tgctttaatt ttaagattaa acttacaaaa
     5161 a
//
LOCUS       DATXQK010000074         5156 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.074, whole genome shotgun sequence.
ACCESSION   DATXQK010000074 DATXQK010000000
VERSION     DATXQK010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5156
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.074"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            101..1649
                     /locus_tag="VC394_001617"
     rRNA            101..1649
                     /locus_tag="VC394_001617"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1701..1773
                     /locus_tag="VC394_001618"
     tRNA            1701..1773
                     /locus_tag="VC394_001618"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1734..1736,aa:Ala,seq:tgc)
     gene            2074..4976
                     /locus_tag="VC394_001619"
     rRNA            2074..4976
                     /locus_tag="VC394_001619"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            5062..>5156
                     /gene="rrf"
                     /locus_tag="VC394_001620"
     rRNA            5062..>5156
                     /gene="rrf"
                     /locus_tag="VC394_001620"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1461 a         1063 c         1481 g         1151 t
ORIGIN      
        1 agacgaacca aacgtgaggg tgatatggag acatattacc cgtcaagaaa cgagaaataa
       61 atctgtcagc gacagaagga acgagtaagt tcaaacgcaa attaaagaga gtttgatcct
      121 ggctcaggac gaacgctggc ggcgtgccta atacatgcaa gtagaacgct gagaactggt
      181 gcttgcaccg gttcaaggag ttgcgaacgg gtgagtaacg cgtaggtaac ctacctcata
      241 gcgggggata actattggaa acgatagcta ataccgcata agagagacta acgcatgtta
      301 gtaatttaaa aggggcaatt gctccactat gagatggacc tgcgttgtat tagctagttg
      361 gtgaggtaaa ggctcaccaa ggcgacgata catagccgac ctgagagggt gatcggccac
      421 actgggactg agacacggcc cagactccta cgggaggcag cagtagggaa tcttcggcaa
      481 tgggggcaac cctgaccgag caacgccgcg tgagtgaaga aggttttcgg atcgtaaagc
      541 tctgttgtta gagaagaatg atggtgggag tggaaaatcc accaagtgac ggtaactaac
      601 cagaaaggga cggctaacta cgtgccagca gccgcggtaa tacgtaggtc ccgagcgttg
      661 tccggattta ttgggcgtaa agcgagcgca ggcggttttt taagtctgaa gttaaaggca
      721 ttggctcaac caatgtacgc tttggaaact ggagaacttg agtgcagaag gggagagtgg
      781 aattccatgt gtagcggtga aatgcgtaga tatatggagg aacaccggtg gcgaaagcgg
      841 ctctctggtc tgtaactgac gctgaggctc gaaagcgtgg ggagcaaaca ggattagata
      901 ccctggtagt ccacgccgta aacgatgagt gctaggtgtt aggccctttc cggggcttag
      961 tgccggagct aacgcattaa gcactccgcc tggggagtac gaccgcaagg ttgaaactca
     1021 aaggaattga cgggggcccg cacaagcggt ggagcatgtg gtttaattcg aagcaacgcg
     1081 aagaacctta ccaggtcttg acatcccgat gcccgctcta gagatagagt tttacttcgg
     1141 tacatcggtg acaggtggtg catggttgtc gtcagctcgt gtcgtgagat gttgggttaa
     1201 gtcccgcaac gagcgcaacc cctattgtta gttgccatca ttaagttggg cactctagcg
     1261 agactgccgg taataaaccg gaggaaggtg gggatgacgt caaatcatca tgccccttat
     1321 gacctgggct acacacgtgc tacaatggtt ggtacaacga gtcgcaagcc ggtgacggca
     1381 agctaatctc ttaaagccaa tctcagttcg gattgtaggc tgcaactcgc ctacatgaag
     1441 tcggaatcgc tagtaatcgc ggatcagcac gccgcggtga atacgttccc gggccttgta
     1501 cacaccgccc gtcacaccac gagagtttgt aacacccgaa gtcggtgagg taacctatta
     1561 ggagccagcc gcctaaggtg ggatagatga ttggggtgaa gtcgtaacaa ggtagccgta
     1621 tcggaaggtg cggctggatc acctcctttc taaggaaatg gaacacgttt atcgtcttat
     1681 ttagttttga gaggtcttgt ggggccttag ctcagctggg agagcgcctg ctttgcacgc
     1741 aggaggtcag cggttcgatc ccgctaggct ccatcaggat acaatcctac taaacttaat
     1801 acaagtgaag ttgaacacgc aactcacttc ctaggaaaat agacaatctt cccttgtgtg
     1861 caaggcccac atggtcagat tcctaatttt ctacagaagt ttcgctaaag cgagcgttgc
     1921 ttggtatcct atataatagt ccattgaaaa ttgaatatct atatcaaatt ccacgatcta
     1981 gaaatagatt gtagaaaagt aacaagaaaa taaaccgaaa acgctgtgac tatttgataa
     2041 gttttctagt ttttaaaaaa actaggttaa taaggttaag ttaataaggg cgcacggtgg
     2101 atgccttggc actagaagcc gaagaaggac gtgacaaacg acgaaatgct ttggggagct
     2161 gtaagtaagc gctgatccag agatgtccga atgggggaac ccactaacta atggttagta
     2221 tccataactg ttaaggttat gagaaggaag acgcagtgaa ctgaaacatc taagtagctg
     2281 caggaagaga aagcaaacgc gattgcctta gtagcggcga gcgaaacggc aggagggcaa
     2341 accgaagtgt ttacacttcg gggttgtagg actgcgacgt gggactttaa aaggatagaa
     2401 gaattacctg ggaaggtaag ccaaagagag taaaagcctc gtatttaaaa ttcttttgag
     2461 ccctagcagt atcctgagta cggcgagaca cgtgaaatct cgttggaatc tgggaggacc
     2521 atctcccaac cctaaatact ctctagtgac cgatagtgaa ccagtaccgt gagggaaagg
     2581 tgaaaagcac cccgggaggg gagtgaaata gaacctgaaa ccgtgtgcct acaacaagtt
     2641 cgagcccgtt aatgggtgag agcgtgcctt ttgtagaatg aaccggcgag ttacgatatg
     2701 atgcgaggtt aagttgaaga gacggagccg tagggaaacc gagtcttaat agggcgattt
     2761 agtatcatgt tgtagacccg aaaccatgtg acctacccat gagcaggttg aaggtgaggt
     2821 aaaactcact ggaggaccga accagggcac gttgaaaagt gcttggatga cttgtgggta
     2881 gcggagaaat tccaaacgaa cttggagata gctggttctc tccgaaatag ctttagggct
     2941 agcgtcgatg ttaagtctct tggaggtaga gcactgtttg ggtgaggggt ccatctcgga
     3001 ttaccaatct cagataaact ccgaatgcca acgagatatc atcggcagtc agactgcgag
     3061 tgctaagatc cgtagtcgaa agggaaacag cccagaccac cagctaaggt cccaaaataa
     3121 ctgttaagtg gaaaaggatg tggggttgca cagacaacta ggatgttagc ttagaagcag
     3181 ctattcattc aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta
     3241 ccggggctaa aacagtttac cgaagctgtg gatgacacaa aagtgtcatg gtaggagagc
     3301 gttctatgtg tgaagaaggt gtaccgtgag gagcgctgga acgcatagaa gtgagaatgc
     3361 cggtatgagt agcgaaagac aggtgagaat cctgtccacc gtaagactaa ggtttccagg
     3421 ggaaggctcg tccgccctgg gttagtcggg acctaaggag agaccgaaag gtgtatccga
     3481 tggccaacag gttgatattc ctgtactaga gtatatagtg atggagggac gcagtaggct
     3541 aactaaaccg gacgattgga agagtccggc taagcagtga ggtgtaagat gagtcaaatg
     3601 cttatcttta taacattgag ctgtgatggg gagcgaattt tagtagcgaa gttagtgatg
     3661 tcacactgcc aagaaaagct tctagcgttt aatgatactc tacccgtacc gcaaaccgac
     3721 acaggtagtc gaggcgagta gcctcaggtg atcgagagaa ctctcgttaa ggaactcggc
     3781 aaaatgaccc cgtaacttcg ggagaagggg tgctgactta ggtcagccgc agtgaatagg
     3841 cccaagcaac tgtttatcaa aaacacagct ctctgctaaa tcgtaagatg atgtataggg
     3901 ggtgacgcct gcccggtgct ggaaggttaa gaggagggtt tagcgcaagc gaagatctga
     3961 attgaagccc cagtaaacgg cggccgtaac tataacggtc ctaaggtagc gaaattcctt
     4021 gtcgggtaag ttccgacccg cacgaaaggc gtaatgattt gggcactgtc tcaacgagag
     4081 actcggtgaa attttagtac ctgtgaagat gcaggttacc cgcgacagga cggaaagacc
     4141 ccatggagct ttactgcagt ttgatattga gtatctgtac cacatgtaca ggataggtag
     4201 gagccattga cttcgggacg ccagtttcga atgaggcgct gttgggatac tacccttgtg
     4261 ttatggctac tctaacccag ataggttatc cctatcggag acagtgtctg acgggcagtt
     4321 tgactggggc ggtcgcctcc taaagagtaa cggaggcgcc caaaggttcc ctcagattgg
     4381 ttggaaatca atcgcagagt gtaaaggtat aagggagctt gactgcgaga gctacaactc
     4441 gagcagggac gaaagtcggg cttagtgatc cggtggtacc gaatggaagg gccatcgctc
     4501 aacggataaa agctaccctg gggataacag gcttatctcc cccaagagtt cacatcgacg
     4561 gggaggtttg gcacctcgat gtcggctcgt cgcatcctgg ggctgtagtc ggtcccaagg
     4621 gttgggctgt tcgcccatta aagcggcacg cgagctgggt tcagaacgtc gtgagacagt
     4681 tcggtcccta tccgtcgcgg gcgtaggaaa tttgagagga tctgctccta gtacgagagg
     4741 accagagtgg acttaccgct ggtgtaccag ttgtcttgcc aaaggcatcg ctgggtagct
     4801 atgtagggaa gggataagcg ctgaaagcat ctaagtgcga agcccccctc aagatgagat
     4861 ttcccatgat tttatatcag taagagccct gagagatgat caggtagata ggttaggagt
     4921 gtaagtgtag cgatacatgt agcggactaa tactaatagc tcgaggactt atccaaaaag
     4981 aaatattgac aacgttacgg attcttgtta gactatagat attcaatttt gagtgggtta
     5041 tgaaaatagc acatttacaa gttaagtgac gatagcctag gagatacacc tgtacccatg
     5101 ccgaacacag cagttaagcc ctagaacgcc tgatgtagtt gggggttgcc ccctgt
//
LOCUS       DATXQK010000075         5089 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.075, whole genome shotgun sequence.
ACCESSION   DATXQK010000075 DATXQK010000000
VERSION     DATXQK010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5089
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.075"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            36..254
                     /locus_tag="VC394_001621"
     CDS             36..254
                     /locus_tag="VC394_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin NrdH"
                     /protein_id="HEP5995070.1"
                     /translation="MITVYSKNNCMQCKMTKKFLEQHGANFQEINIDEHPEKVDYVKS
                     LGFTSAPVIEADNLVFSGFQPAKLKELI"
     gene            274..2433
                     /gene="nrdE"
                     /locus_tag="VC394_001622"
     CDS             274..2433
                     /gene="nrdE"
                     /locus_tag="VC394_001622"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEP5995071.1"
                     /translation="MSLKDIGDISYFRLNNEINRPVNGKIPLHKDKEALKAFFAENVL
                     PNTMSFTSITEKIEYLISNDYIESAFIQKYRPEFITELDSIIKSENFRFKSFMAAYKF
                     YQQYALKTNDGEYYLENLEDRVLFNALYFADGQEDLAKDLAVEMINQRYQPATPSFLN
                     AGRSRRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     AGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGITVPDKFYELARKNEDMYLFSPYNVEKEYGIPFNYLDITNMYDELVANPKI
                     TKTKIKARDLETEISKLQQESGYPYIINIDTANKANPIDGKIIMSNLCSEILQVQTPS
                     LINDAQEFVEMGTDISCNLGSTNILNMMTSPDFGRSIKTMTRALTFVTDSSSIEAVPT
                     IKHGNSQAHTFGLGAMGLHSYLAQHHIEYGSPESIEFTDIYFMLLNYWTLVESNNIAR
                     ERQTTFVGFENSKYANGSYFDKYVTGHFVPKSDLVKSLFKDHFIPQASDWEALRDAVQ
                     KDGLYHQNRLAVAPNGSISYINDCSASIHPITQRIEERQEKKIGKIYYPANGLSTDTI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFLRSELPMELYEWKTQSKQTTRDLS
                     ILRNYAFNKGIKSIYYIRTFTDDGEEVGANQCESCVI"
     gene            2766..3725
                     /gene="nrdF"
                     /locus_tag="VC394_001623"
     CDS             2766..3725
                     /gene="nrdF"
                     /locus_tag="VC394_001623"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEP5995072.1"
                     /translation="MTTYYEAINWNEIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSLQEKDLVGKVFGGLTLLDTMQSETGVEAIRADVRTPHEEAVLNNIQFMESVHAK
                     SYSSIFSTLNTKKEIEEIFEWTNNNEFLQEKARIINNIYANGDALQKKVASTYLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEDEQENFR
                     DWMYDLLYQLYENEEKYTKTLYDGVGWTEEVMTFLRYNANKALMNLGQDPLFPDTAND
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGSVEAMSDDDYNYGL"
     gene            3700..5013
                     /locus_tag="VC394_001624"
     CDS             3700..5013
                     /locus_tag="VC394_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015017188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEP5995073.1"
                     /translation="MTIIIMDSNSAHETDNLSVSFLNFCYNSLMKRHFLLLTFYLFLT
                     GLTAGLVAFILTKAIHLIQSLSFGFSQGSFSTMIASVPPQRRALSLLFAGLLAGLGWH
                     LLAKKGKDIQSIQQIIQDDISFSPWTQFWHGWLQLTTVSMGAPVGREGASREVAVTLT
                     SLWSQRCNLSKADQKLLLACASSAALGAVYNAPLATILFILEAILNRWSLKNIYAACL
                     TSYVAVETVALLQGRHEIQYLMPQQHWTLGTLIWSVLAGLILSLFAHAYKHLLEHLPK
                     ADAKSQWFIPKVLIAFSLIAGLSIFFPEILGNGKAGLLFFLHEEPHLSYISWLLVAKA
                     VAISLVFASGAKGGKIAPSMMLGGASGLLLAILSQYLIPLSLSNTLAIMVGATIFLGV
                     INKIPLAAPVFLVEITGQSLLMIIPLALANLIFYFSYQFYRFILK"
BASE COUNT         1512 a         1029 c          906 g         1642 t
ORIGIN      
        1 agatggaacc attactaatt ttagaaggac aagacatgat tacagtatat tccaaaaata
       61 attgcatgca gtgcaaaatg actaagaaat ttttagaaca acacggagcc aacttccagg
      121 aaattaatat cgatgaacat cctgaaaaag tggattatgt gaaaagtctt ggcttcacat
      181 ctgctcctgt tatcgaagca gataacctcg tattttctgg cttccaacct gcaaagttaa
      241 aagaattgat ttaataagac aaggaaaaat aatatgagtc tcaaagatat tggcgatatt
      301 tcatattttc gcctaaataa tgaaattaac cgtcctgtta atggtaaaat tccacttcat
      361 aaagacaaag aagctttaaa agcttttttc gctgaaaatg tgctgccaaa caccatgtct
      421 tttacttcca ttacggaaaa aattgagtat ttaatctcaa atgattacat tgaatcagct
      481 tttattcaga aataccgtcc tgaatttatt actgaattag atagcataat caaatcagaa
      541 aattttcgct ttaaatcatt tatggcagcc tacaagttct accagcaata cgccttaaaa
      601 acaaatgatg gagagtatta tttagaaaac cttgaagacc gtgtcttgtt taatgctttg
      661 tattttgcag atggtcaaga agacttagca aaagatttag ccgttgaaat gattaaccaa
      721 cgttaccaac cggctactcc ttccttttta aatgctggtc gaagccgtcg tggtgaattg
      781 gtctcttgtt tcttgattca agtaactgat gacatgaatt ctatcggacg ttccattaac
      841 tctgccttgc aattatcccg tattggtgga ggtgttggga ttaccttgtc taacctccgt
      901 gaagctggcg caccaatcaa aggctatgct ggtgcagcct caggagttgt tcctgttatg
      961 aaattatttg aagatagttt ttcttattca aatcaacttg ggcaacgtca aggagctggt
     1021 gttgtttacc taaatgtttt tcatcctgat atcattgctt tcttatctac taaaaaagaa
     1081 aatgccgatg aaaaggtgcg tgttaaaacc ttgtcactag ggattaccgt tcctgataaa
     1141 ttctacgaat tagctcgtaa aaacgaggac atgtatctct ttagtcctta caatgttgaa
     1201 aaagaatatg gcattccctt taactatctc gacattacca atatgtacga tgagttagtg
     1261 gcgaacccta aaattactaa gactaaaatt aaagctcgtg atcttgaaac agagatttca
     1321 aaattacaac aagaatctgg ttacccttat atcatcaata ttgatacagc taataaagct
     1381 aatcctatcg atggaaaaat catcatgagc aacttgtgtt ctgaaatttt acaagttcaa
     1441 acacctagcc ttatcaatga tgcgcaagag tttgtagaaa tgggaactga tatttcatgt
     1501 aacttaggtt ccactaatat cctgaacatg atgacctcac cagactttgg ccgttctatt
     1561 aagaccatga cacgtgccct aacttttgtt actgattcat caagcattga agctgttcca
     1621 accattaaac atggcaatag ccaagctcat acttttggcc ttggagctat gggactacat
     1681 tcttaccttg ctcaacatca tattgaatat ggcagtccag aatccatcga gtttactgat
     1741 atttacttta tgctcctgaa ttattggacc ttggtcgaat ccaataacat cgctcgtgag
     1801 cgccaaacta cctttgttgg ctttgagaac tctaagtacg ctaatggtag ttactttgat
     1861 aaatacgtta caggacactt tgttccaaaa tctgatttgg tcaaaagtct ctttaaagat
     1921 cactttattc cgcaagcttc agattgggag gctcttcgcg acgccgttca aaaagatggt
     1981 ctttatcatc aaaaccgtct agcagttgct ccaaatggct ctatttctta tatcaatgac
     2041 tgctctgctt ctattcaccc aatcacacaa agaattgaag aacgtcaaga aaagaaaatt
     2101 ggtaaaatct actatcctgc aaatggtttg tctacggata ccattcctta ctatacatct
     2161 gcttacgata tggacatgcg caaagttatt gatgtctatg ccgctgcgac cgaacatgtg
     2221 gaccaaggct tgtcattaac tctattcctt cgtagtgagt tgcctatgga gctttatgag
     2281 tggaaaacac aaagcaaaca aaccactcgt gatttatcca tcttacgaaa ctacgctttc
     2341 aataaaggca ttaaatctat ctactatatc cgtaccttta cggatgatgg ggaagaagtg
     2401 ggcgcaaacc aatgtgaatc ttgtgtcatt taattggtta agaagtaatg atgctaaagc
     2461 atctattact tctacggcag tcgctttagg cgatctgcct aaaccactca ctaactacgt
     2521 aaggaaagta ttatcgactt tccttactcc gtgtcgtaat tcctcgatat gccctaatga
     2581 tgctaaagca tctattactt cttggacaat cgctttaggc gttccgccta aaccactcac
     2641 tgactcattc gttacccttt tgaatctatt taaacttatt attaccggta gaactttggt
     2701 cggttctact ggtttatctc gatatcctta acgcacttta ctactcttta ggagatcatt
     2761 tttctatgac tacatattat gaagcaatta actggaatga aatcgaagac gtcattgaca
     2821 agtcaacttg ggaaaagttg accgaacaat tttggcttga tacccgtatc cctttatcaa
     2881 atgacttaga cgattggcgc aaactttctc ttcaagaaaa agatcttgtt ggcaaggttt
     2941 ttggaggatt aaccctactt gataccatgc aatcagaaac tggtgtcgaa gctattcgtg
     3001 ctgatgttcg cactcctcac gaagaagctg tcttaaacaa tattcaattt atggaatcag
     3061 ttcatgctaa atcatactcg tctatctttt caaccttaaa caccaaaaaa gaaattgaag
     3121 agatctttga gtggactaat aataatgagt tccttcaaga aaaagcacgt attatcaata
     3181 acatttacgc taatggagat gccttacaaa agaaagttgc ctcaacatat ctcgaaactt
     3241 tcctttttta ttctggcttt ttcacccctc tttactattt gggaaataat aagctagcaa
     3301 atgttgctga aatcattaaa ctaattattc gtgatgaatc tgtacatggt acttatatcg
     3361 gttacaaatt ccagcttggt tttaacgaac taccagaaga tgagcaagag aattttcgtg
     3421 attggatgta tgacctcctt tatcagctgt atgaaaatga agaaaaatac accaaaacgc
     3481 tttatgatgg cgtagggtgg actgaggaag ttatgacctt tttacgctac aatgctaaca
     3541 aagctcttat gaatttagga caagatcctt tattcccaga tactgcaaac gatgttaacc
     3601 caattgttat gaatggtatt tcaacaggaa catcaaacca tgactttttc tcacaggtag
     3661 ggaatggcta tcttcttggt tctgtagaag ctatgtctga tgacgattat aattatggac
     3721 tctaatagtg ctcacgagac tgataatcta tcagtctctt ttcttaactt ttgttataat
     3781 agtcttatga aacgacactt tttactcttg accttttacc tctttttaac aggtttaaca
     3841 gctggccttg ttgcttttat cttaacaaaa gccattcacc tgattcagtc cctcagtttt
     3901 ggattcagtc aaggttcttt tagcactatg attgctagcg ttccaccaca aagacgagct
     3961 ctttcacttc tctttgctgg acttcttgca ggtcttgggt ggcacctatt ggctaaaaaa
     4021 ggaaaggaca ttcaatctat tcagcaaatc attcaagatg acatctcatt tagtccctgg
     4081 acacagtttt ggcatggttg gctccaatta acgaccgtat ctatgggagc tcctgtcggc
     4141 cgagaaggag cttcacggga agtcgctgtt accttgacgt ctctttggag ccaaagatgc
     4201 aacttgtcaa aagccgatca aaaactttta ttagcttgcg cttctagcgc tgcccttggt
     4261 gctgtctaca atgctccctt agccactatt ttatttatct tggaagctat tcttaaccgt
     4321 tggtccctta aaaatatata cgctgcctgc ctaacgagct atgtggctgt ggaaacagtt
     4381 gctttattac aaggccgaca tgagattcaa tacctaatgc ctcagcaaca ttggacgcta
     4441 ggaaccctta tttggtccgt cctggctggt cttatccttt ccctctttgc tcacgcttac
     4501 aaacatcttt tggaacacct tcctaaggct gatgccaaaa gtcagtggtt tattccgaaa
     4561 gttctcatcg cttttagcct tatcgcagga ctcagcattt tcttcccaga gattttagga
     4621 aatggcaaag ctggattact cttttttctc catgaagaac cacatctgag ctatattagc
     4681 tggctacttg ttgccaaagc tgtcgctatc tctttagttt ttgcttctgg agcaaaaggg
     4741 ggtaaaattg ctccttctat gatgcttgga ggtgctagtg gcttactact agctatcctt
     4801 agtcagtacc ttattccctt gtctctatca aatacgttag ccattatggt tggtgcaaca
     4861 atctttttag gtgtgatcaa taagataccc ttggcggcac cagtttttct ggttgaaatt
     4921 acaggccaat cgttactaat gattatccct ttagcattag ctaacctcat tttttatttt
     4981 agctatcagt tttatcgttt catccttaaa tgattagtta attacttttt taataaaaag
     5041 cttcggatga attggacttg gtatactaaa gtagacacaa aattaagtg
//
LOCUS       DATXQK010000076         5067 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.076, whole genome shotgun sequence.
ACCESSION   DATXQK010000076 DATXQK010000000
VERSION     DATXQK010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5067
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.076"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..381
                     /locus_tag="VC394_001625"
     CDS             <1..381
                     /locus_tag="VC394_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HEP5995074.1"
                     /translation="FGALSYGITNLFPLYVCFFFFVYISNASKITINIVEKMGIIFVI
                     SAIIGATVLAMYLTWTPVGSSTVLGVQSRYLIGIIPLVLLLFSSQQQKFKQIEDILSD
                     KLAIHVSLLFILAMLMSTIFRYYH"
     gene            475..1698
                     /gene="pepT"
                     /locus_tag="VC394_001626"
     CDS             475..1698
                     /gene="pepT"
                     /locus_tag="VC394_001626"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEP5995075.1"
                     /translation="MKYDNLLDRFIKYVKVNTRSDPDSETTPSTESQEAFALTILKPE
                     MEAIGLQDVHYNPVNGYLIGTLPANNPTLTRKIGFIAHMDTADFNAENVNPQIIDNYQ
                     GGDITLGSSNYKLDPKAFPNLNNYIGQTLITTDGTTLLGADDKSGIAEIMTAIEFLTS
                     QPQIEHCDIKVAFGPDEEIGVGADKFEVADFEVDFAYTMDGGPLGELQYETFSAAALE
                     VTFLGRNVHPGTAKDQMINALELAIDFHEKLPAKERPEYTDGYQGFYHLTGLTGTVEE
                     ARASYIIRDFEEASFEARKVKVENIAQSMNAQLGTKRVLVELNDQYYNMKKVIEKDMT
                     AIELAKEVMEELAIKPVIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEFVSLQT
                     MERAVDVIIGLVCKA"
     gene            1739..2227
                     /locus_tag="VC394_001627"
     CDS             1739..2227
                     /locus_tag="VC394_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HEP5995076.1"
                     /translation="MIKLFGKIRYHWQPELSWSIIYWSIAFAPIFVGLSLLYERTEIP
                     SRVFILFAIFAVLVGIGLHRYFIIENNGILRIVSFKLFGPRKLLISTITKIEVTKSTL
                     CLHFEDKSYLFYMRKWPKKYFLDALAVNPYFQGEVILSDNFIKLDYFEVYQHDKKALT
                     RG"
     gene            complement(2214..2411)
                     /locus_tag="VC394_001628"
     CDS             complement(2214..2411)
                     /locus_tag="VC394_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEP5995077.1"
                     /translation="MKVSIIPEKCIACGLCQTYSSLFDYHDNGIVTFSSSSETSQSIC
                     PSDKDAILAVKSCPTKALTLE"
     gene            2460..2936
                     /locus_tag="VC394_001629"
     CDS             2460..2936
                     /locus_tag="VC394_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEP5995078.1"
                     /translation="MAKEPWEEKIVDDTIGTRTRKSRNAFISTPWLTALLSVFFVIIV
                     AILFIFFYTSNSGSNRQAETNGFYGASTHKKTRKASNAKKASSSSTTTDTTPSSEETL
                     ASSEGTGETLTVLAGEGAASIAARAGISVEQLQALNPEHMTQGYWYANPGDQVTIK"
     gene            2951..3631
                     /locus_tag="VC394_001630"
     CDS             2951..3631
                     /locus_tag="VC394_001630"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEP5995079.1"
                     /translation="MKAIKIAIDGPASSGKSTVAKIIAKNLGYTYLDTGAMYRSATYI
                     ALTHGYTGKEVALILEELEKNPISFKKAKDGSQLVFLGDEDVTLAIRQNDVTNNVSWV
                     SALPEIREELVHQQRRIAQAGGIIMDGRDIGTVVLPDAELKIFLVASVEERAERRYKE
                     NLEKGIESDFETLKEEIAARDYKDSHRKVSPLKAAEDALIFDTTGVSIDGVVQFIQEK
                     AEKIVDMS"
     misc_feature    3655..3778
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            3793..4323
                     /gene="infC"
                     /locus_tag="VC394_001631"
     CDS             3793..4323
                     /gene="infC"
                     /locus_tag="VC394_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEP5995080.1"
                     /translation="MKIIAKKDLFINDEIRVREVRLVGLEGEQLGIKPLSEAQSLADA
                     SNVDLVLIQPQAVPPVAKLMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPVIDKGD
                     FETKLRNGRKFLEKGNKVKVSIRFKGRMITHKEIGAKVLADFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPISDKK"
     gene            4365..4562
                     /gene="rpmI"
                     /locus_tag="VC394_001632"
     CDS             4365..4562
                     /gene="rpmI"
                     /locus_tag="VC394_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEP5995081.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAFTSHRFHGKTKKQRRHL
                     RKAGLVSSGDFKRIKAMVTGL"
     gene            4621..4980
                     /gene="rplT"
                     /locus_tag="VC394_001633"
     CDS             4621..4980
                     /gene="rplT"
                     /locus_tag="VC394_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEP5995082.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVAD
                     AAAFTALADAAKAKLGK"
BASE COUNT         1640 a          839 c         1042 g         1546 t
ORIGIN      
        1 tttggcgcat taagctatgg tataacgaac cttttcccgc tttatgtgtg tttcttcttt
       61 tttgtttata ttagtaatgc tagtaaaata actattaata ttgtagaaaa aatgggtata
      121 atctttgtta tttcagcgat tataggtgca actgtattag ctatgtacct gacttggaca
      181 ccagtagggt cctcaactgt cttaggtgtt caaagtcgtt atctgatagg aattatccca
      241 ttagtgttgc ttctttttag tagtcaacag caaaagttca aacaaataga ggatatcctt
      301 tctgacaaac tggctattca tgtttcgctt ttgtttattc tcgcaatgtt aatgagtact
      361 atttttagat attatcatta aactcttaat tagaggcttt aaaaaactta gcttgaataa
      421 gtattttaaa gccttttatg ctataattaa attgagaaat tattgaggtg gcctatgaag
      481 tacgataact tgttagatcg atttataaaa tatgtcaaag tgaatacaag aagcgatcca
      541 gacagtgaga ctactcctag tacggagagt caagaagcat ttgccttaac gattttaaag
      601 cctgaaatgg aggcaattgg tttgcaagat gttcattata atccagttaa tggctatctt
      661 attggaactc tgcctgccaa taatccaacc ttgacgcgta aaattggttt tatagcacat
      721 atggatacgg ctgatttcaa tgctgaaaat gttaatccgc agattattga caattatcaa
      781 ggaggggaca ttacacttgg cagttctaat tataagttgg atccaaaagc atttccaaat
      841 cttaataatt atatcggtca aacattgatt accactgatg ggactacact tttaggggca
      901 gatgataaat ctggtattgc tgaaatcatg acagcaatag aattcttaac atcacaacca
      961 caaatagagc actgtgacat taaagtggct tttggccctg atgaagagat tggggtcgga
     1021 gctgataagt ttgaggtggc tgattttgag gtggattttg cttatactat ggatggcggt
     1081 cctttaggcg aattgcaata tgaaactttc agcgctgcag ctctagaagt gactttttta
     1141 ggcagaaatg ttcatcctgg tacggctaag gatcaaatga ttaatgccct tgagttagca
     1201 atagattttc atgagaagct acctgctaaa gagcgacctg aatacacaga tggttatcaa
     1261 ggcttctatc acttaaccgg actaactgga acagttgaag aggcacgcgc atcttacatt
     1321 attcgtgatt ttgaagaagc atcatttgaa gctagaaaag tgaaagttga aaatatagct
     1381 caatcgatga atgctcagct aggaacaaaa cgtgtcttgg tagagcttaa tgaccaatac
     1441 tacaatatga aaaaagttat cgaaaaagat atgactgcta ttgagttagc taaagaagta
     1501 atggaagagc ttgctatcaa gcctgtaatc gaacccatac gtggaggaac agatggctct
     1561 aaaatttcat ttatgggtat tcccactcct aatatttttg cagggggtga gaatatgcac
     1621 gggcgttttg agtttgttag cttacaaacg atggagagag ctgtcgatgt cattattgga
     1681 cttgtttgca aagcctaatt atcatgtaat tgatatttag tggaaggggc ttataaaaat
     1741 gattaaacta tttggtaaaa taaggtatca ctggcagcca gagttgtctt ggtctattat
     1801 ttattggtct attgcgtttg cacctatttt tgtaggttta tctttgttgt atgagcgcac
     1861 tgaaatacct agtcgggttt ttattctatt tgctattttt gctgtcctag ttggcattgg
     1921 attacatcgt tatttcatta ttgaaaataa tggtatttta agaatcgtat cttttaaatt
     1981 gtttggtcca cgtaagctac ttatttctac tattactaag attgaggtga ccaaatcaac
     2041 actttgtctg cactttgaag ataaaagtta tctcttttat atgcgcaaat ggcctaaaaa
     2101 atattttctt gatgccttag cggttaatcc atacttccaa ggagaagtta tattaagtga
     2161 taactttatt aaattagact attttgaagt ttatcagcat gataaaaaag cccttactcg
     2221 agggtaagag ctttagttgg gcaagatttg acagctaaaa tagcgtcctt atctgaagga
     2281 caaattgact gacttgtttc agatgagctt gaaaatgtga cgataccatt gtcatgataa
     2341 tcaaaaagtg atgaataggt ttggcataga ccacaagcga tgcatttttc tggaatgata
     2401 gatactttca taactattat tctaatatga ttttgttaaa ttaacaaggg gggacgatca
     2461 tggctaaaga accatgggaa gaaaaaattg ttgatgatac tatagggaca cgaacacgta
     2521 aatcaagaaa tgctttcatt agcacgcctt ggttgactgc tttattaagt gtattctttg
     2581 tcatcattgt tgctatactt tttattttct tttatacatc aaatagcggt agtaatagac
     2641 aagctgaaac aaatggtttt tatggagcat ccactcataa aaaaacaagg aaagcttcta
     2701 acgctaaaaa agcatcaagt agttcaacaa ctacagacac aacaccttct agcgaagaaa
     2761 cacttgcttc tagtgaagga actggcgaaa cccttactgt attggcaggt gagggggcag
     2821 cttctattgc agcccgtgca ggtatttctg tggagcagtt acaagcactt aatccagagc
     2881 acatgactca aggatattgg tatgccaatc caggagatca agtcactatt aaataattga
     2941 agaggaaaaa atgaaagcaa ttaaaattgc gattgatggg ccagcttcaa gtggaaaaag
     3001 tacagtagcc aagattattg caaaaaatct tggctatact tatctagata ctggtgctat
     3061 gtatcgctca gcaacttata tcgcactgac acatggctat acgggtaaag aagtagccct
     3121 tattttagag gaacttgaga aaaatcctat ttcctttaaa aaagcaaaag atggctccca
     3181 attagttttt cttggggatg aggacgtgac cttagccatt aggcaaaatg atgtgactaa
     3241 caatgtttct tgggtctcag ctttgccaga aattagagaa gagctggttc atcaacaaag
     3301 acgcattgca caagctggtg gcattatcat ggatggtcgt gacattggaa cggttgttct
     3361 cccagatgcg gaactaaaaa tctttttagt ggcttctgtc gaagagcggg cagagcgccg
     3421 ctataaagaa aacctagaaa aaggaattga atcagatttt gagacgctaa aagaagagat
     3481 tgcagctcgt gattacaagg atagtcatcg taaggtttca cctctaaaag cagctgaaga
     3541 tgcccttatt tttgatacca caggtgtttc tattgatggc gtggtgcaat ttatccaaga
     3601 aaaagcagaa aaaatagttg acatgtccta gagcctatgt tagaatatta acaatgagaa
     3661 aagtagaagt gagagcttct cgccttgcga cttaggttgt ctggccctaa catgtcaaat
     3721 tcctatggaa tattatgtgt gcgggcttga tttatcaagt ccgctttcgt ttttctctaa
     3781 aaaaataaag aggtgaagat catagctaaa aaggatctat tcattaatga tgaaattcgc
     3841 gttcgcgaag ttcgtctcgt tggtcttgaa ggtgaacaat taggtattaa accattgtct
     3901 gaagcgcaat cattggctga tgcttcaaat gttgatttgg ttttaatcca gccacaagct
     3961 gttcctcctg ttgccaaact tatggactat ggaaagttca aatttgagta tcaaaagaaa
     4021 cagaaagaac aacgtaaaaa acaaagcgtt gtgactgtga aagaagttcg tctcagtcca
     4081 gttatcgata aaggtgactt tgaaacaaaa cttcgtaacg gtcgtaaatt ccttgaaaaa
     4141 ggaaataaag ttaaggtttc tattcgtttc aaaggacgta tgattactca taaagagatt
     4201 ggtgccaagg tactagctga ctttgctgaa gcgactcaag atattgctat cattgagcaa
     4261 cgagcaaaaa tggatggacg ccaaatgttt atgcaacttg caccaatttc agacaagaag
     4321 taattgtcac agtttcatta tatctttaag aggagaatac taaaatgcca aaacaaaaaa
     4381 cacaccgcgc atcagctaaa cgttttaaac gtacaggttc aggcggtttg aaacgcttcc
     4441 gcgcctttac atcacaccgt ttccacggaa aaactaaaaa acaacgtcgt catcttcgta
     4501 aagctggttt ggttagctca ggagatttca aacgtatcaa agcaatggtt actggtcttt
     4561 aattttaaag gcagtagaca tttacgagta ttataagaat tatttgaagg agaactatta
     4621 atggcacgtg ttaaaggtgg cgttgtttca cgcaaacgtc gtaaacgtat tttaaaatta
     4681 gctaaaggtt actatggagc aaaacacatc ttgttccgta ctgcaaaaga gcaagtgatg
     4741 aactcttact actatgcata ccgtgatcgc cgtcagaaaa aacgtgattt ccgtaaacta
     4801 tggatcactc gtatcaatgc ggctgctcgt atgaacggtt tgtcatactc acaattgatg
     4861 cacggtttga aacttgctga aatcgaagta aaccgtaaaa tgcttgctga tttagcagta
     4921 gctgatgcag cagctttcac agctcttgct gatgcagcca aagcaaaact tggcaaataa
     4981 gaaacgctat acctaaaaaa taactttcta ttacctatag aaagttataa agaaacaaaa
     5041 atgaaggaga cgatggacgt cttcttt
//
LOCUS       DATXQK010000077         4846 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.077, whole genome shotgun sequence.
ACCESSION   DATXQK010000077 DATXQK010000000
VERSION     DATXQK010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.077"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(183..1451)
                     /locus_tag="VC394_001634"
     CDS             complement(183..1451)
                     /locus_tag="VC394_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEP5995083.1"
                     /translation="MTTKYQTIISNIEQDIQKQRLKKGDKLPSIRVLSKVYHCSKDTV
                     QRALLELKYRHLIYAVPKSGYYVLGNVSMPDNVLNLSLEDYNNMAYEDFRLCLNEALS
                     AKDKYLFHYYHKTEGLEELREALLLYLAENSVYSNKDQLLITSGTQQALYILSQMPFP
                     NAGKTILLEKPTYHRMEAIVAQLGLPYQTISRHFNGLDLELLESLFQTGDIKFFYTIS
                     RFSHPLGLSYNTKEKEAIVRLAQRYQVYILEDDYLGDFVKLKEPPIHYYDTHHRIIYL
                     KSFSMSVFPALRIGALVLPSGLKPYFLTQKSLIDLDTNLLMQKALALYLENGMFQKNL
                     RFIKRYLKQRERQLALFLKQNCPDIHYQLTPTHLVIDYTTSDSYRNFTLDKSDRIIIT
                     GKKRYLSIAINQQIQSKLNSLIKNTCGKSN"
     gene            1541..2407
                     /locus_tag="VC394_001635"
     CDS             1541..2407
                     /locus_tag="VC394_001635"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HEP5995084.1"
                     /translation="MKRIIVANDLVGVGKVALSASIPLMASCLLEQVLLPTCLLSSHT
                     GGGQSPVKHTTSSILDVFLTDWDNKRIKFDGIFIGYFTTTRDGQKLLEWLSRKQLPLI
                     LDPIMADNGKLYQGFSQKHIEIMIKLTQKAELVLPNISEACLLTQTAYLAKNYDEEDI
                     RQVARKLAKLGPKQVVISGISFEEGSIGFAYYQSDSDQLTFHFGKQFPYHFYGTGDIA
                     SAIITAGFYYQLPLTQILDLVVSFLEKVLQNTLTLKRDLILGVAYESHLNYLTKSFKK
                     LLEEKNVISKIS"
     gene            2385..2942
                     /locus_tag="VC394_001636"
     CDS             2385..2942
                     /locus_tag="VC394_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEP5995085.1"
                     /translation="MSSQKLANYIELSMYAALIFISVQVLRIPIGMQFIHLGNALVVI
                     AVLIYGSKKGALVASLGLGIFDVLNGYASVVWITILESLAICLILHLVYERAMQSSKK
                     PKAIITITVIAALSKLTINLIKYLLLAYIGTQLPLSVSFWVALGKIGGSFGTSFVTLV
                     AVPLLYPVIKNLKEKVTIGQAKRGF"
     gene            3076..4620
                     /locus_tag="VC394_001637"
     CDS             3076..4620
                     /locus_tag="VC394_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEP5995086.1"
                     /translation="MSILEVKQLSHGFGDRAIFENVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGHLQPDEGKVEWSKYVTAGYLDQHTVLESGQTVRDVLRTAFDELFKTENRIN
                     EIYASMADDKADIAVLMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMESDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNHLDAEHIEWLKRYLQHYENAFVLISHDI
                     SFLNDVINIVYHVENQSLVRYTGDYYQFQAVYEMKQSQLEAAYERQQKEIANLQDFVN
                     RNKARVATRNMAMSRQKKLDKMDIIELQAEKPKPNFEFKQARTPSRFIFQTKNLVIGY
                     DYPLTKEPLNITFERNQKIAIVGANGIGKSTLLKSLLGVIEPLEGHIVTGDFLEVGYF
                     EQEVTGVNRQTPLEVVWDAFPALNQAEVRAALARCGLTSKHIESQIQVLSGGEQAKVR
                     FCLLMNRENNVLILDEPTNHLDIDAKNELKRALKAYKGSILMVCHEPDFYNGWVTDTW
                     DFSKLT"
BASE COUNT         1569 a          716 c          925 g         1636 t
ORIGIN      
        1 tttttaagta atctaaaatt atgttttttt aatttcaaaa tcatgctttc gtttgttata
       61 aaaaccgatc ttccatagtt agatttttta tccaggcaat tgaagattgg ggcaactatt
      121 caattagaga ttttataata cttatttatc cccatttttt atattaatgg tacgaagaaa
      181 agttagttgc tctttccaca agtatttttg attagcgagt ttaatttgct ttgtatttgt
      241 tgattgatag caatagagag atagcgcttt tttccagtta ttattattct atcagattta
      301 tctaatgtaa agtttctata agagtctgat gtcgtgtaat caatcactaa atgagtaggg
      361 gttagttgat agtgaatatc agggcaattt tgttttaaaa agagagccaa ttgacgttcc
      421 cgctgtttta gataacgctt aataaagcga agattttttt gaaacatccc attttctaaa
      481 taaagagcta atgctttttg cattagtaag ttcgtgtcta aatcaatcag tgatttttgg
      541 gttaaaaaat aaggttttaa acctgacggt aaaactagag ctccgatacg gagtgcagga
      601 aaaacactca ttgaaaaaga ttttaggtag atgattcggt gatgagtgtc ataataatga
      661 attggtggct ctttaagttt cacaaaatct cctaaataat catcttctaa aatataaacc
      721 tggtatcgtt gtgctagacg aacaatagct tccttttcct tagtgttata agaaagtccc
      781 agaggatgtg aaaatcgtga aatcgtgtaa aaaaatttaa tatcgccagt ttgaaataaa
      841 gattctaaga gttctaaatc aaggccatta aagtgtctag agatagtttg atagggtaat
      901 cctaactgag caacaatagc ttccatccga tgataagtag gcttttcaag gagaattgtt
      961 ttgcccgcat taggaaaagg catttgtgat aaaatataaa gcgcttgttg cgtcccagag
     1021 gtgattaata gttggtcttt gttactgtaa acactatttt cagctaagta aagaagcaaa
     1081 gcttccctta gttcttctag tccttcggtt ttgtgataat aatgaaagag gtatttgtct
     1141 ttagcactta aagcctcatt taagcagagt cggaaatctt cataagccat attattataa
     1201 tcttcaagac ttagattaag aacattatcg ggcatgctta cgttacccaa aacataataa
     1261 ccacttttag gaacagcata aattaagtga cgatatttta attctaaaag agcccgctga
     1321 acagtatctt tgctacaatg ataaacctta cttaataccc taattgaagg tagcttgtcc
     1381 ccttttttta gtcgctgttt ctggatatct tgttcgatat tggaaatgat tgtttgatat
     1441 tttgttgtca taaattgtcc ccatacagtt aagtgttata actattgtat cttatgcctc
     1501 tttttcttac aatagttatt atagaaaaga ggtaatagct atgaaacgga ttattgtggc
     1561 aaatgatctg gttggtgttg gtaaggtagc tttgtctgct agtattcctt taatggcttc
     1621 ttgtttgtta gagcaagttt tattaccaac ttgcctttta tcaagccata ctggcggagg
     1681 acagagccct gtgaagcata ctacatcaag tatattagac gtttttttaa cagattggga
     1741 caacaaaaga attaagtttg acggcatctt tataggttat tttacgacaa caagagatgg
     1801 gcaaaagcta ctagaatggc tttcaagaaa gcaacttccc ttaattttag atccaattat
     1861 ggcagataat ggaaaacttt atcaaggctt cagtcaaaaa catatagaga ttatgataaa
     1921 acttactcaa aaagctgagc ttgttttacc taatattagt gaggcttgtc tattaacaca
     1981 aacagcttat ttagcaaaaa attatgatga agaagatatt agacaggtgg ctagaaaatt
     2041 agctaagctt ggacctaaac aggttgtcat tagcggaatc tcttttgagg agggcagcat
     2101 tggttttgct tactatcagt cagattcaga tcaattaaca ttccattttg ggaagcaatt
     2161 cccttaccat ttctatggta caggagatat agcatcagct attataacag caggattcta
     2221 ttatcagcta ccactaacac aaattctaga tcttgttgtt tcatttcttg aaaaagtatt
     2281 gcaaaatacc cttacattaa aaagagattt aatcttaggt gttgcttatg aatctcattt
     2341 aaattactta actaaatcgt ttaaaaagtt gttggaggaa aaaaatgtca tctcaaaaat
     2401 tagctaatta tattgaattg agtatgtatg ccgctttaat ttttatttca gtacaagtgt
     2461 tgcgtatccc tatcggtatg cagtttattc atcttggcaa tgcattagtt gttattgcag
     2521 tcttgatcta tggtagtaaa aaaggagctc tagttgctag tcttggttta gggatctttg
     2581 atgttttgaa cggctatgct tcagtcgttt ggataactat cttagaatcc ttggctattt
     2641 gtttaatttt gcatttggta tatgaaagag ctatgcaatc tagtaaaaaa ccaaaagcca
     2701 ttataacaat aacagttatt gctgcattgt caaaattaac tattaacctt ataaaatact
     2761 tactgttagc ttatataggt actcagctac cactttcggt atctttttgg gtagctcttg
     2821 gaaaaattgg tggtagtttc ggaacatctt ttgtgacttt agttgctgtt cctcttttgt
     2881 atcctgttat caaaaactta aaagaaaagg ttacaatcgg acaagcaaaa agaggttttt
     2941 gatacaagta ccttatcgat aaaaaagcaa ggtacacttt cagtcaaata ttgacaaaag
     3001 gttacaattt tgccataatg aaataactat aaagccgttt tgacggcttt tttgatgaca
     3061 gttaaggaag aaacgatgag tattttagaa gttaagcagt tgagtcacgg ttttggggat
     3121 cgggctattt ttgaaaatgt gtcatttcgc ctcttaaaag gtgaacatat tggactagtt
     3181 ggagcaaatg gtgaaggaaa atcaaccttt atgagtatag tcacaggaca tttacagcct
     3241 gatgaaggaa aagtagagtg gtcgaagtat gtcactgcag gttacctgga tcaacataca
     3301 gtgttggaat caggacaaac cgttcgtgat gtcttgcgaa ctgcttttga tgagttattt
     3361 aagaccgaga atcgtattaa tgagatttac gcgtcaatgg cagatgataa agctgatatt
     3421 gctgttttga tggaagaagt aggtgagctt caagatcgtt tagaaagtcg tgatttctat
     3481 actttggatg ctaagattga tgaagtagcg cgtgcgcttg gtgttatgga ttttggaatg
     3541 gagtcagatg ttacatcctt atcaggtggg caacgaacaa aggttttatt agccaaatta
     3601 ctattagaaa aacctgatat tctgctatta gatgaaccaa ctaaccattt ggatgctgag
     3661 catattgaat ggttaaaacg ctatttacaa cattatgaaa atgcttttgt gttgatttcg
     3721 catgatattt ctttcttaaa tgatgtgatt aatattgttt accatgttga aaatcaaagt
     3781 ttagttcgct atactgggga ttattaccaa tttcaagctg tttatgagat gaaacaatct
     3841 caacttgaag cagcctatga acgtcaacaa aaagagattg ctaacttgca ggattttgtc
     3901 aaccgaaata aggctcgggt agcgacacgt aacatggcaa tgtctcgcca aaaaaaactt
     3961 gataagatgg atataattga acttcaagct gagaaaccaa aaccaaattt tgaatttaag
     4021 caagctagaa ctcccagtcg attcattttt caaacaaaaa atcttgtgat tggttatgat
     4081 tacccattga ccaaagaacc cttaaatata acgtttgaaa gaaaccaaaa aattgctatt
     4141 gttggggcca acggtattgg aaaatctact ttgctaaaaa gtttattagg tgttatcgag
     4201 cctttagaag gtcatattgt cacaggggat tttttagaag ttggctactt tgaacaagaa
     4261 gtgacaggtg ttaaccgaca aactccgcta gaagtagttt gggatgcttt tcctgcctta
     4321 aatcaggcag aagttcgagc ggcactagct cgttgcggac taacatcaaa acatattgaa
     4381 agtcaaattc aagtactttc gggtggtgaa caagcaaaag ttcgtttttg tttgttgatg
     4441 aatcgtgaaa ataacgtgct tattttagac gaaccaacaa atcatcttga tattgatgct
     4501 aaaaatgagc tcaaacgtgc tttaaaagca tataagggtt ctattttaat ggtttgtcat
     4561 gaacctgatt tctacaatgg gtgggtaacc gatacttggg attttagtaa gttaacctaa
     4621 actattcctt aattttttac acaggaatta ctaaaatcag taaagatata gaataaatac
     4681 tgctagatat gtgatggttt aggtaactga tcaccaacca aaaaagatgt gactacatct
     4741 tttttttatc taatggaatg gtgtaaagtc gtttaataat agcttatttt ttgatgatca
     4801 gttgttcatg aaattgttgg tgattaacaa gtcatttaag atacaa
//
LOCUS       DATXQK010000078         4845 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.078, whole genome shotgun sequence.
ACCESSION   DATXQK010000078 DATXQK010000000
VERSION     DATXQK010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4845
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.078"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            1..633
                     /locus_tag="VC394_001638"
     CDS             1..633
                     /locus_tag="VC394_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEP5995087.1"
                     /translation="MIKIDEIHRILGIDEVYKAPKRLTDILFDKDSREDIFRQFLKYE
                     TDVSYDWFMQYFEEEQADRKNKKQDFTPKSVSTLLSKIISGNQYYEVAVGTGGILIQA
                     WQEQRLNDSPFTYRPSKYWYHVEELSDKAVPFLLFNMSIRGINGVVVHGDSLTRQVKN
                     IYFLQNTKDDMLSFSDINVMPRTQDIEREFNVKEWIGDAIVHVESKLNAR"
     gene            636..1346
                     /locus_tag="VC394_001639"
     CDS             636..1346
                     /locus_tag="VC394_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEP5995088.1"
                     /translation="MNIKEAKIGDKVWVEGIINSSFSNGGVHVNHAGVDAFYDLEQTK
                     IKPDGYAGQSPKYLKNIIAKMRELPEHDRAVWIKGIFKEFGIENDISYYLGHKQGVLQ
                     AKDSLKPEVPQYVADYIEKCKKRGDTLYLALKVGKSVKQSVYKWLWDGSGDESFIKFA
                     KAWIYGYTIEKEKLYTVEIPNPNGLSVCLQRLDDGNVVLSIPRNARWKGLEVMQLTES
                     EIRKDFDWAWQFAEEVRE"
     gene            1343..1714
                     /locus_tag="VC394_001640"
     CDS             1343..1714
                     /locus_tag="VC394_001640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5995089.1"
                     /translation="MKKLTLSLLLNLVVIFGLGVIDIKLTMWQAAVLGIVSYYLSSWI
                     SKRKDSKEIGVLLLELPAIGKAKPYHFLYLHQNDELPEHNYFTISITFLKERVISGAY
                     KCTSKEAEKFPQFKWVPLTKL"
     gene            1982..2419
                     /locus_tag="VC394_001641"
     CDS             1982..2419
                     /locus_tag="VC394_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEP5995090.1"
                     /translation="MGNIPTPKANNFLEELKTIPHLIETLERDANLMSRSLVKSPQWS
                     DMRVSGGVKQSQEDKNIKMLHMVSYYSDQIERLKDRRQEMANLIVQSMGICESHVLLT
                     TYLDCDGDYERARERLNIGNRNKYFMFVRRGKESLELILKNTN"
     gene            complement(2938..3195)
                     /locus_tag="VC394_001642"
     CDS             complement(2938..3195)
                     /locus_tag="VC394_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5995091.1"
                     /translation="MLSLLPTPFWDTSIYRTGTVGIEPTHLLLALRKEFSDLAIVPKQ
                     GATLTVFQSRFMFQRFRRAKTSHKRSSTDLARIERATALNI"
     gene            3276..3794
                     /locus_tag="VC394_001643"
     CDS             3276..3794
                     /locus_tag="VC394_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HEP5995092.1"
                     /translation="MGFVDKKLSEITPYKNNPRNNDEAVGPVAESIKEFGFKVPIVVD
                     KNGEIINGHTRYKAAQKLGLETVPVIVADDLSEEQIKAFRLADNKVGEIAVWDLDLLN
                     EELNDILDLDMSAFGFELEIDDENQENLDADFEEIEDDSVLIVEAESEEELEKLYDEF
                     VERGFKCRVSIL"
     gene            3773..4450
                     /locus_tag="VC394_001644"
     CDS             3773..4450
                     /locus_tag="VC394_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5995093.1"
                     /translation="MPSFNIVKKNDLNSSFKVSKVMADFDVGAEHVREHFIGKIDYPD
                     KWQIGLIVGGSGTGKSTIAKELYNEALEDDFVYPDKPVIDCIPCHNVEELQKMFYAVG
                     FGSVPSWLKPYHVLSNGEKMRVDLARRILTKEFIVFDEFTSVVDRQVAKIICIALKKA
                     LKRYPDKKFIAVGCHHDVIEFLQPDWCFNTDDMQQVFRFPHEANKNSQSEDAQLPSGE
                     NLDVIII"
     gene            4366..4842
                     /locus_tag="VC394_001645"
     CDS             4366..4842
                     /locus_tag="VC394_001645"
                     /inference="COORDINATES: protein motif:HMM:NF012792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEP5995094.1"
                     /translation="MPPRSKQEFTVRRCSIAEWGKFRRYHYLNGDIVRAARCFGLYDD
                     DKIIGFIGVIHFPHPKNKKIKRVTRLVILPDYQGVGLGTRFLKSVAEIYSCQGFDFRI
                     VTSAKNLINALNRNTNWKLKSYDKGNTPTGNSSIKQLAKTTRKNVKIASFLFIRKN"
BASE COUNT         1657 a          743 c         1055 g         1390 t
ORIGIN      
        1 atgattaaga tcgatgagat acatcgcata ctaggcatcg acgaagttta taaagcaccc
       61 aaacgactta cggacatact ctttgataaa gatagtcgtg aggatatatt tagacagttt
      121 ttgaaatatg aaacagatgt atcttacgac tggtttatgc aatattttga ggaagaacaa
      181 gctgaccgca aaaataaaaa gcaagacttt acgcctaaat cggttagtac actattatct
      241 aaaataataa gtggtaatca atactacgag gtagcagtcg ggacgggtgg gatacttatc
      301 caagcatggc aagaacaacg attaaatgac agtccattta cttatcgtcc gagtaaatac
      361 tggtatcacg tagaagagtt atcggacaaa gcagtaccgt ttctactctt taatatgtct
      421 ataagaggaa taaatggtgt ggtggtgcat ggcgactctt taacaagaca agttaaaaac
      481 atttattttt tgcaaaatac aaaagacgac atgctgagtt ttagtgatat taacgttatg
      541 ccaaggactc aagatattga gcgagaattt aatgtcaaag agtggattgg tgatgcgata
      601 gtgcatgtag agagtaagtt aaatgcgagg taaaaatgaa cattaaagaa gcgaaaatag
      661 gcgataaagt gtgggttgaa ggtattatca attcgtcctt ttcaaatggt ggagtacatg
      721 taaatcatgc tggtgttgac gccttttatg atcttgagca aacaaaaata aaaccagacg
      781 gatacgcagg gcagtcacct aaatatctta aaaatattat tgctaaaatg cgagaactac
      841 cagagcacga cagagccgtc tggattaaag gtatttttaa ggaatttggt attgagaacg
      901 acattagcta ttatcttggc cacaagcaag gcgtattaca agcaaaggat tcgctaaaac
      961 cagaagtacc gcagtatgtg gctgactata tagaaaagtg caagaaaaga ggcgacacat
     1021 tatacttagc gttaaaagtc ggaaaatctg taaaacaatc tgtatataag tggttatggg
     1081 atggcagcgg tgatgagagt tttattaagt ttgctaaagc ttggatttac ggttacacaa
     1141 tcgaaaaaga aaagctttat actgttgaga taccgaatcc gaatgggctt tccgtctgct
     1201 tgcaacggtt agatgacgga aacgttgttt tatcaatacc tcgcaacgca aggtggaaag
     1261 gtctggaagt tatgcaatta accgaatcag aaatccgtaa agattttgat tgggcgtggc
     1321 agtttgcgga agaggtgagg gaatgaaaaa acttacacta tcactactat tgaacctggt
     1381 tgtaatcttt ggattaggtg ttatagatat aaaattaact atgtggcagg cagctgtttt
     1441 aggtattgta tcctactatt tatcaagttg gatttcaaag aggaaggata gtaaagagat
     1501 tggagtttta ttacttgagt tgccagcaat aggcaaagct aaaccatatc actttttgta
     1561 tctccaccaa aatgatgagt taccagagca caattacttt acaatttcaa taacattttt
     1621 aaaagagcgt gtcatatccg gtgcttataa atgcaccagc aaagaagcag agaagtttcc
     1681 acagtttaaa tgggtaccgc tgacaaaatt ataaccccac gcaagcgctc aagagcctgc
     1741 aatggctctg tgggggtgga ccgaaattaa aaaatagaaa cgaggacctc cttacaccaa
     1801 aacaaatcta acgcagatta tcggtcattt gttattattc aaggcgctaa tactgacatc
     1861 gtacgcctgt gtcgaaaaac aaaaaaagaa agagaggact tttctccaca aaaaaagacg
     1921 tccatgcgaa cgtcttcatg attaaatacc caacaatatt atatcatgaa tggagagtta
     1981 gatgggcaac attccgacac caaaagctaa taattttttg gaagaattaa agactatccc
     2041 acatctcata gagacccttg aaagagacgc taacctaatg agtcggtcgc tcgttaagtc
     2101 tcctcaatgg tctgatatgc gagtgtctgg tggggttaaa caatcacagg aagacaaaaa
     2161 cataaagatg ctgcatatgg ttagctatta tagtgatcag attgagcgtt taaaagaccg
     2221 tcgacaagaa atggctaatt tgattgtgca aagcatgggc atttgtgaga gtcacgtttt
     2281 actcacgact tatcttgact gtgatggaga ctatgagaga gccagagaac gcttaaacat
     2341 aggaaatcgt aataaatact ttatgtttgt taggcgagga aaggaaagtc tggaattgat
     2401 actaaaaaat actaattaga tacaaattga tactacataa tactaatagc tgtgttaata
     2461 ttgtagtata gcaaaaacta cgatcaaact tgttatttta tggtatgtgg gacgtgcagg
     2521 ttcgaatcct gccgtctcaa tcacccagag attacacgct gtgacattgc ggaatgtaat
     2581 caaagcaaaa agaatcacgg aactttgcga tgtccaccgt gaattaacac ccttatttga
     2641 cattggcgtt cctttgagga gaatacgcaa tctgggttcg gtgttaatga ttaagactta
     2701 gcaacgcctc ttaacatgcg taccagcgct aagtcgattg attaaccgca agtaaaacaa
     2761 gggtcgcaac cttgcttgtg gttagctgat agttcggttg aggattcggc cgaagtgagt
     2821 tgttgcactg tgcacggtgt gataagctat cgtgggctta agttttgtgg cgaaacggca
     2881 ccgttaataa tatttagact gtttatttgg acttgcgttc gcattaggaa atactcacta
     2941 aatattgagt gcagtggcac gttcgattcg tgcaaggtct gtagaagagc gtttgtgaga
     3001 ggtcttagct cttctaaacc tttggaacat gaaccgtgat tggaaaacgg taagggtagc
     3061 gccttgcttc gggacaatag ctaagtccga aaactctttt cgaagagcca gaagaagatg
     3121 tgtcggttcg attccgactg ttcctgttcg ataaatagaa gtgtcccaga atggggtagg
     3181 caataggctt agcattcatt cgctatttat ctatagttaa ccaattagtc atcacattgt
     3241 ggtgactttt tattatgtaa aaaaaggaat aaaacatggg atttgtggac aaaaaattaa
     3301 gtgaaataac accttataaa aataacccta gaaacaatga tgaggctgtt ggaccagttg
     3361 ctgaatctat taaagagttt ggttttaaag tgcctattgt cgttgataaa aacggcgaaa
     3421 tcataaacgg ccacactcgt tataaagcag cccaaaaatt agggctagaa acagttcctg
     3481 ttattgtcgc agatgactta tctgaagagc aaatcaaagc gttcagactt gctgacaata
     3541 aagttggtga gattgcggtt tgggatttag atttgttaaa tgaagaatta aatgacattt
     3601 tagatcttga tatgtctgct tttggatttg aattagaaat agatgacgag aatcaagaga
     3661 atttagatgc tgattttgaa gaaatagaag atgactctgt tttaatagta gaagctgaaa
     3721 gcgaagagga attggaaaag ttatatgacg aatttgtaga aagaggtttc aaatgccgag
     3781 tttcaatatt gtaaaaaaga acgacttaaa tagttctttt aaagtttcca aagtaatggc
     3841 tgactttgat gtcggcgcag aacacgttag agaacatttt attggaaaaa tagactatcc
     3901 cgataaatgg cagataggtt taattgtagg cggaagtgga acaggaaaaa gcactatcgc
     3961 taaagagttg tataacgaag cgttagaaga tgactttgtt tatcctgata aacctgttat
     4021 agattgcata ccatgtcaca atgttgaaga attacaaaaa atgttctatg cggttgggtt
     4081 tgggagtgtt ccttcttggc tcaagcctta tcatgtttta tcaaatggcg aaaaaatgcg
     4141 tgttgattta gctaggcgaa tattaacaaa agagtttatc gtttttgatg agtttactag
     4201 tgttgttgat agacaagtcg ctaaaataat ttgtatagcg ttaaaaaaag ctttaaaaag
     4261 atatcctgac aagaaattta tcgcagttgg ttgtcatcat gatgtaattg agtttttaca
     4321 accagattgg tgttttaata cagatgatat gcaacaggtt tttcgattcc cccacgaagc
     4381 aaacaagaat tcacagtcag aagatgctca attgccgagt ggggaaaatt tagacgttat
     4441 cattatctaa atggagatat agttagagcc gcacgttgtt ttgggctata tgatgatgat
     4501 aaaataatag gatttattgg ggtaatacac tttccacatc ctaaaaataa aaaaatcaaa
     4561 agagttacta ggttggttat tttacctgat tatcaaggtg taggattagg tactagattt
     4621 ttaaaatcag tagctgaaat atattcttgt caagggtttg actttcggat tgtcacgagc
     4681 gctaaaaatc ttattaatgc gttaaataga aatactaatt ggaaattgaa aagttacgat
     4741 aaaggaaata ctccaacggg aaattcttcg attaaacaac ttgcaaaaac aactagaaaa
     4801 aatgttaaaa tagctagctt tctttttata agaaaaaatt aactc
//
LOCUS       DATXQK010000079         4745 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.079, whole genome shotgun sequence.
ACCESSION   DATXQK010000079 DATXQK010000000
VERSION     DATXQK010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4745
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.079"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..1201
                     /locus_tag="VC394_001646"
     CDS             <1..1201
                     /locus_tag="VC394_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hyaluronidase"
                     /protein_id="HEP5995095.1"
                     /translation="SNQGMRVELESKISGLQSTQQATARQISQEISNREGAVSRVQQG
                     LDSYQRRLQNAEGSYSSLQQTVSGLQSDVNSPNSKLNSRISQLASQIDQRVTRADVTS
                     IINQSGDSIKLAIQRAGGIDAKMSAKEIVSAINLNGYGVRISGERIALDGNTTVNGAF
                     GAKIGEFIKLKADQIIGGTIDANKINVINLNASSIVGLDANFIKARIEYAITSLLEGK
                     VIKARNGAMTIDLQSGQINHYTNESAMRRIDSSTASQFIKMTKSGFISEIGNMQAAMT
                     VIGSNSDGSENHENKTFGGIRIWNGKSSYQSTSFVELVGNRVAIYGNKNRSPWLFDST
                     TSGYAYLIPQNESGIKHAIGRADRKIDQIHVGDIYVQGERVAMMLKDLANRIGYIGTG
                     GWANRIG"
     gene            1215..1376
                     /locus_tag="VC394_001647"
     CDS             1215..1376
                     /locus_tag="VC394_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5995096.1"
                     /translation="MQEKLLGKIINDLTLKVANLTLENAQLKAQHEIELEELNAQLDE
                     ATAPKEEGK"
     gene            1379..1996
                     /locus_tag="VC394_001648"
     CDS             1379..1996
                     /locus_tag="VC394_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5995097.1"
                     /translation="MRNWKVTGNYPQWDGTGAVIDTQVIITDDRGAVIAEKIKKDLRT
                     ANDAEIIEAALEEFKKTAYVEIAMGEAVQKVDDLEKISQETAKTAKTAQTAAGLAKVS
                     AERTQKMINLQTIHMLTSGGKIDPDIYKGMLELIEPAKKGEYQAYDVFTVVDSTKEED
                     GEAGEGNLVFVHVNEPFTYEAQTLKDLESEDKVTVIKYADLVKQD"
     gene            2007..2303
                     /locus_tag="VC394_001649"
     CDS             2007..2303
                     /locus_tag="VC394_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5995098.1"
                     /translation="MIDFVQIGAFCGAALSILGVWGFIVNPFKKAMEANEFAMAQLKD
                     SIKELAYELKNLDREREITKKIIDRHEERLGRVEDEVIINKERIITLFKKGEEK"
     gene            2300..2485
                     /locus_tag="VC394_001650"
     CDS             2300..2485
                     /locus_tag="VC394_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5995099.1"
                     /translation="MNNWFKKVAIKTIKTMAQTAVGLIGSSMLITDINWPTMLSAVLL
                     SGLTCILMNVSQIKEEE"
     gene            2597..3931
                     /locus_tag="VC394_001651"
     CDS             2597..3931
                     /locus_tag="VC394_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysin"
                     /protein_id="HEP5995100.1"
                     /translation="MATYQEYKSRSNGNAYDIDGSLGAQCWDGYADYCKYLGLPYANC
                     TNTGYARDIWEQRHKNGILNYFDEVETMQAGDVAIFMVVAGVTPYSHVAIFDSDAGSG
                     YGWFLGQNQGGANGAYNLVKIPYSTTYPTAFRPKVFKNAVTVIGNIGLNKGDYFIDVS
                     AYQQADLTATCQQAGTTKTIIKVSESLAWLSDRHQQQANTSDPIGYYHFGRFGGDSNL
                     AQREADLFLSNLPTKKVSYLVIDYEDSASADKEANTNAVIAFMDKIANAGYKPVYYSY
                     KPFTLNNIDYQQIIAKYPNSIWIAGYPDYEVRTEPLWEFFPSMDGVRWWQFTSVGVAG
                     GLDKNIVLLADDSSKVDIPKIDKPQSQLTFNQKLDTNTKLDNSNVPYYEATLRTDYYV
                     ESKPNASSADKEFIKAGTRVRVYEKVNGWSRINASQSDQWVEDKYLSNATQV"
     gene            complement(4007..4714)
                     /gene="speC"
                     /locus_tag="VC394_001652"
     CDS             complement(4007..4714)
                     /gene="speC"
                     /locus_tag="VC394_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021733756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptococcal pyrogenic exotoxin SpeC"
                     /protein_id="HEP5995101.1"
                     /translation="MKKINIIKIVFIITVILISTISPIIKSDSKKDISNVKSDLLYAY
                     TITPYDYKDCRVNFSTTHTLNIDTQKYRGKDYYISSEMSYEASQKFKRDDHVDVFGLF
                     YILNSHTGEYIYGGITPAQNNKVNHKLLGNLFISGESQQNLNNKIILEKDIVTFQEID
                     FKIRKYLMDNYKIYDATSPYVSGRIEIGTKDGKHEQIDLFDSPNEGTRSDIFAKYKDN
                     RIINMKNFSHFDIYLEK"
BASE COUNT         1598 a          786 c         1021 g         1340 t
ORIGIN      
        1 tagtaatcaa ggtatgcgcg tagagctgga gtcaaaaatc agtgggttgc aatcaacgca
       61 acaagcaact gccaggcaaa tctcacaaga gataagtaac cgtgaaggtg ctgtcagtcg
      121 tgtacaacag ggcctagaca gttatcagcg gcgattacaa aatgctgaag ggagttacag
      181 cagcttacaa caaactgtta gtggtttaca gtctgatgtt aatagtccaa acagtaagct
      241 taattctcgg attagccaat tagctagtca aattgaccaa cgagtcacaa gagccgatgt
      301 gacaagcatc attaaccagt ctggggacag tatcaagtta gccatacaga gagctggtgg
      361 gattgatgct aagatgtccg caaaagagat tgtctcagcc ataaacctca atggctacgg
      421 tgtcagaatt tctggtgagc gtattgcttt agacggcaat accacggtta acggggcttt
      481 tggcgcaaaa attggtgagt ttatcaagct aaaagccgac cagattatcg gtgggacaat
      541 cgatgcaaac aaaatcaatg tgattaatct caacgctagc agtattgttg gtttagacgc
      601 taattttatt aaagcaagga ttgagtatgc gatcacaagt ttgcttgagg gtaaagttat
      661 taaggctcgt aatggtgcga tgacaataga cttgcaaagc ggtcaaatca accattacac
      721 aaatgagtca gccatgagac gtatcgatag cagtacagct agtcagttta taaagatgac
      781 aaagtcaggc tttatatctg agataggtaa catgcaagct gcaatgacgg ttataggctc
      841 taatagcgat gggtcagaaa accatgaaaa taaaaccttc ggaggcataa gaatttggaa
      901 cgggaagtca tcttatcaat cgacaagttt tgttgaatta gtcggtaacc gtgtagcaat
      961 ctatggaaat aaaaatcgta gtccatggct ctttgactca acaacgtcag gatatgctta
     1021 cctcattcca cagaatgaaa gtggtataaa gcatgccata gggagagctg atcgtaagat
     1081 agaccaaatc cacgttggag atatctatgt gcaaggcgaa cgtgtcgcta tgatgttaaa
     1141 agatttagcg aataggattg gctatatagg tacaggtggc tgggctaacc gcataggata
     1201 aattaggaga aacaatgcaa gaaaaattat taggaaaaat tattaacgat ttaacactta
     1261 aagtcgctaa tttaacgctg gaaaatgctc aattaaaagc acagcatgaa atcgaattgg
     1321 aagaattaaa cgcacaattg gatgaagcaa cagcaccgaa ggaagaaggt aaataaacat
     1381 gagaaattgg aaagtaacag gaaactaccc acagtgggac ggtacaggag cagtcattga
     1441 tacgcaggta attattacag atgacagggg tgcagtcatc gcagagaaga tcaaaaaaga
     1501 tttgagaaca gctaatgatg cagaaattat cgaggctgct ttagaggagt ttaaaaagac
     1561 tgcttacgtc gaaatcgcta tgggcgaagc cgtgcaaaaa gtagacgacc ttgaaaaaat
     1621 ctcacaggaa actgctaaga ctgccaaaac tgctcaaaca gctgctggac tagctaaggt
     1681 gtccgcagag cgcacgcaaa agatgattaa cttacaaacg atccacatgt taacgagcgg
     1741 cggcaagatt gatcctgaca tttataaagg catgcttgag ctaatcgagc cagccaaaaa
     1801 aggtgagtat caagcgtatg atgtctttac ggtggtcgac agtactaaag aggaagacgg
     1861 tgaagcaggc gaagggaacc tagtcttcgt acacgttaac gagccgttta cttatgaggc
     1921 gcaaactctt aaagatttgg aatcagagga taaagtcaca gtcattaaat atgcggattt
     1981 ggttaaacaa gattagaggt ggttagatga tagattttgt acaaattggt gctttttgtg
     2041 gcgctgcttt gtctatcttg ggtgtttggg gatttatcgt caatcccttt aaaaaagcca
     2101 tggaagctaa tgagtttgcc atggctcaac tcaaggactc aattaaggag ttagcttatg
     2161 agcttaaaaa tcttgatcgt gaacgtgaga ttaccaaaaa aattatcgac agacacgagg
     2221 agcgtttagg tcgcgtagaa gacgaagtta ttatcaacaa ggagcgtatt attacgctat
     2281 ttaaaaaagg agaagaaaaa tgaataactg gtttaaaaaa gtagcaatta aaacaattaa
     2341 aacaatggca caaacggccg ttggtcttat cgggtcaagc atgttgatta cggatattaa
     2401 ctggccaaca atgttgtcag cagtgctgtt gtcaggacta acatgtatcc tgatgaatgt
     2461 gtcacaaatc aaggaagagg aataggtcat gcgagcaatc acacgattag cattagttat
     2521 agcaatcgca atactgtatg tgccattatc tgtggttgct ttgatttttt atccattttt
     2581 agataaggag ggataaatgg ctacatatca ggaatataaa agtcgttcaa atggcaatgc
     2641 ttacgatatt gatggatcgc ttggtgcgca atgttgggat ggctacgcag actactgtaa
     2701 gtatctagga ctgccatacg caaactgtac aaatacagga tatgcaaggg atatatggga
     2761 acaacgtcac aaaaatggta ttttaaacta ctttgacgaa gtagagacta tgcaagctgg
     2821 agatgtcgca atttttatgg tagttgcagg tgttacaccg tatagccatg tggctatttt
     2881 tgatagtgat gcaggtagtg gatacggatg gtttttaggt caaaaccagg gtggagcaaa
     2941 cggagcatat aatttagtaa aaataccata ttcaacgact tatcctactg catttagacc
     3001 aaaagttttt aaaaatgcag ttactgttat aggtaatata ggactaaata aaggcgatta
     3061 ttttattgat gtatcagctt atcaacaagc agacttaacc gctacttgtc agcaagctgg
     3121 cactactaaa acgattatca aagtatccga gtcactcgct tggctgtctg ataggcatca
     3181 gcaacaagct aatactagtg accctattgg ttattatcac tttggacgat ttggaggaga
     3241 tagcaactta gcgcaacgag aagcagattt atttctgtcc aatttaccaa ccaaaaaagt
     3301 ctcttactta gtcattgatt atgaagactc tgcaagtgcc gacaaagaag ctaacactaa
     3361 tgcagttatt gcgtttatgg ataaaattgc aaacgctgga tataagcctg tttattacag
     3421 ctataaacca tttacgctta ataatattga ttatcagcaa attatcgcta agtacccaaa
     3481 cagcatttgg atagctggtt atccagacta cgaagtacga acagagccac tttgggagtt
     3541 cttcccttca atggatggtg tgcgctggtg gcagttcaca agtgtaggag tagcaggtgg
     3601 tttagataaa aatattgtat tattagcaga tgatagtagc aaagtggata tacctaagat
     3661 tgacaaacca caaagccagc ttacttttaa tcaaaagcta gatactaata ctaaattaga
     3721 caactcgaat gtaccttact acgaagcaac ccttagaaca gactattatg tagagtctaa
     3781 gccaaacgca agtagcgctg ataaagaatt tatcaaggca ggaactcgtg taagagtcta
     3841 tgaaaaagtg aatggatggt cacgtattaa cgcttctcag tctgaccagt gggtcgaaga
     3901 taagtattta tctaatgcaa cacaagtata aaataggagg taaagctcct ttagataaga
     3961 caaaaccgct cagataattt ctgggcggtt ttttgtgtat gatgaattat ttttcaagat
     4021 aaatatcgaa atgactaaag ttcttcatat tgataattct attatcttta tattttgcaa
     4081 aaatatctga tctagtccct tcatttggtg agtcaaataa gtctatttgc tcatgtttcc
     4141 catcttttgt gccaatttcg attctgccgc ttacataagg agaagtagcg tcataaattt
     4201 tataattatc cataaggtat tttctgattt taaagtcaat ttcctggaaa gttacgatat
     4261 ctttttctag aataattttg ttatttaagt tctgttgaga ttctcccgaa ataaatagat
     4321 ttcccaataa tttatgattt actttattat tttgagcagg cgtaattcct ccatagatgt
     4381 actcaccggt gtgagaatta agaatataaa ataatccaaa aacatctaca tgatcatctc
     4441 gtttaaattt ttgagaggcc tcataagaca tttcggaact aatataatag tctttccctc
     4501 tatatttttg agtatcaatg tttaatgtgt gtgtcgttga aaaatttacc ctgcaatctt
     4561 tataatcata aggagttata gtgtatgcat aaagtaaatc acttttaaca ttcgaaatgt
     4621 ctttcttaga gtcacttttg atgataggtg aaatagtaga aatcagtatg actgtaatta
     4681 tgaaaactat tttgatgatg ttaatctttt tcattttttc tccttaatta aatgtattac
     4741 tcgta
//
LOCUS       DATXQK010000080         4592 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.080, whole genome shotgun sequence.
ACCESSION   DATXQK010000080 DATXQK010000000
VERSION     DATXQK010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4592
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.080"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(293..656)
                     /locus_tag="VC394_001653"
                     /pseudo
     CDS             complement(293..656)
                     /locus_tag="VC394_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558053.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1077..1610)
                     /locus_tag="VC394_001654"
     CDS             complement(1077..1610)
                     /locus_tag="VC394_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002949762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEP5995102.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENALIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGVSYYCNLASPYMMDTEALKYI
                     DYDLDVKVFADGEKRLLDVDEYEIHKKEMQYSADMDFILKENVKILVDWINHEKGPFS
                     KAYITIWYKRYLELKNR"
     gene            complement(1690..2466)
                     /gene="recX"
                     /locus_tag="VC394_001655"
     CDS             complement(1690..2466)
                     /gene="recX"
                     /locus_tag="VC394_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEP5995103.1"
                     /translation="MKITKIEKKKRLYLIELDNDDSLYVTEDTIVRFMLSKDKVLDND
                     QLEDMKHFAQLSYGKNLALYFLSFQQRSNKQVADYLRKHEIEEHIIADIITQLQEEQW
                     IDDTKLADTYIRQNQLNGDKGPQVLKQKLLQKGIASHDIDPILSQTDFSQLAQKVSQK
                     LFDKYQEKLPPKALKDKITQALLTKGFSYDLAKHSLNHLNFDQDNQEIEDLLDKELDK
                     QYRKLSRKYDGYTLKQKLYQALYRKGYNSDDINCKLRNYL"
     gene            2581..3936
                     /gene="rlmD"
                     /locus_tag="VC394_001656"
     CDS             2581..3936
                     /gene="rlmD"
                     /locus_tag="VC394_001656"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEP5995104.1"
                     /translation="MVVKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIFC
                     QITSVKRNFAEAKLLMVNKASKNRVKPACSVYETCGGCQIMHLAYPKQLDFKDDVIRQ
                     ALKKFKPTGYEQFEIRPTLGMKKPDHYRAKLQFQLRSFGGTVKAGLFSQGSHRLVPID
                     NCLVQDQLTQDIINKITQLVDKYKLPIYNERKIAGIRTIMVRKAQASDQVQIIVVSSK
                     EVRLANFIGELTKAFPQVKTVALNSNRSKSSEIYGDETEILWGQEAIHEEVLDYGFAL
                     SPRAFYQLNPQQTEVLYGEVVKALDVGSKDHIIDAYCGVGSIGFAFAGKVKSVRGMDI
                     IPEAIEDAQKNAKAMGFDNAYYEAGKAEDIIPKWYKQGYRADAIIVDPPRTGLDDKLL
                     KTILHYQPKQMVYVSCNTSTLARDLVQLTKVYDVHYIQSVDMFPHTARTEAVVKLQKR
                     V"
BASE COUNT         1399 a          728 c          956 g         1509 t
ORIGIN      
        1 ctatttagta actttggtta aagtaatcga actaacttgg ttgattgtat tttaatgata
       61 aaaagcttaa atttgttata caacaaattt aagcttttta ttgtttcttt ttaattgcag
      121 attgaggttt aaaagacact ttacaatgtt ctcaagtcat gtgttgttgt aggttgtatg
      181 cacatattac aaatcccatt atagaagaaa ttgtttaatt aatggatagt aatggatggt
      241 atggagatct taatgtggtt ttaatggtaa ttttagcagt tatagtaaga caaaaggtga
      301 cttggaaaag cttatgcaga cgattcttaa ctcccacaat atcttctgtc agattttatt
      361 agacttcttc tcagtgtcaa ttttgtctgt tttacgaacg tgcaggctat tcaattgctt
      421 cttagcttct aaaggattaa accgagtata agcgtagcca ttatctgcga aaaaacctga
      481 agacgacgag aatagacatt aatcgtttta aggataatct cagggttatg gacggttttc
      541 aagtttccaa gaagatgatt gaggccgatg gtatcgtttt gtcttgaatg attccttatt
      601 ttagtggttt cattttcaat acacgacgtc aagcgtccta catactttga taaaattcta
      661 cctaaaagag gtgtcttgtc agttttggtt acaacatcaa gtgccaaaag actttgcgac
      721 ttaacaagac acttctactt taacgcaaag aaaaagtagt gagtactctc tttcgtcgga
      781 gatttcctcg ctactaaact tagtattttt tgatactttc cttaggtagt gcttaaggca
      841 gcgactttct ttggaattcc atgccttatt tttgagatcc taggctcaaa aatgccaagt
      901 ggcgtcaaca ttaacattga cgccactttt accacaacga aaaacatctg ttttggctca
      961 ctccacacgc agaaatagga aattggatgt caaatcagca gagctgttca tgctttttga
     1021 gcctaaaact gagtttgtct gcgttctgag cactccttgg gagtgctgga caactcttaa
     1081 cgattcttca gttcaagata acgtttgtac caaatagtga tataggcctt ggaaaatggt
     1141 cctttttcat gattaatcca atcaaccaat attttgacat tttcttttag gataaagtcc
     1201 atatcggctg aatactgcat ctctttttta tgaatctcgt attcatcaac atcaagtaat
     1261 ctcttttcac catccgcaaa gactttgaca tctaaatcgt aatcaatgta tttgagggcc
     1321 tcagtgtcca tcatgtatgg actagctaag ttacagtaat aagagacgcc attatctcga
     1381 atcatggcta taatgttaaa ccagtatttt ttatgaaagt aaacgattgc aggttcgcgg
     1441 gtcacccatc gtctaccgtc actttctgtg acaagagtgt gatcattaac tccaatgagg
     1501 gcattttcag ttgtttttag taccatagta tcgcgccaag tacggtgcaa actaccatca
     1561 tgcttataac tttgaattgt aataaagtcg ccttctttag gtaatttcat gccttaccaa
     1621 ctttctacaa ttaaacttat cctctatagt ctatcataaa tgtgtgaaaa aatcacttct
     1681 tttgtcagtc tataaataat ttcttaactt gcaattaata tcgtcgctgt tgtagccttt
     1741 tcggtagaga gcctgataga gcttttgctt taaagtataa ccatcatatt tgcgactgag
     1801 tttacgatat tgtttgtcta attctttgtc aagaagatct tctatttctt gattatcttg
     1861 gtcaaaatta aggtgattga ggctatgttt agctagatcg tatgaaaagc ctttggtcag
     1921 taatgcttgg gtgattttat ctttcaaggc ttttggtggc aatttttctt gatatttgtc
     1981 aaagagtttt tggcttactt tttgagcgag ttggctaaag tcagtttgag ataagatagg
     2041 atcaatgtca tgacttgcaa tgcctttttg taataacttt tgttttaaga cttggggacc
     2101 tttatcacca tttaactgat tttggcgaat gtaggtatca gccaatttgg tgtcgtctat
     2161 ccattgttct tcttggagtt gagtgatgat gtcagcaata atgtgttctt caatctcatg
     2221 cttgcgcagg taatcagcaa cttgcttgtt gctgcgttgt tgaaaggaaa gaaaataaag
     2281 ggctaaattt ttgccgtagg acagttgggc aaaatgtttc atgtcttcaa gctgatcatt
     2341 gtcaaggact ttatctttac tcaacataaa ccgaacaata gtatcttctg ttacataaag
     2401 ggaatcgtca ttatccaatt cgataaggta gaggcgtttt ttcttttcaa ttttagtgat
     2461 tttcatatgt tcattatatc aaaaatggta aaatagaaga cgaaaatact gctaaaaatg
     2521 tgttgcttgt gaatgctttt ggtggtgaga ttaggatatt agaggaagta aggggaagag
     2581 atggtcgtaa aggttaaaca gaaaattcct ttaaaaatta agcgaatggg aattaacggt
     2641 gaggggattg gtttttatca gaaaactttg gtatttgtcc ctggtgcttt aaagggagaa
     2701 gatatttttt gccaaatcac gtcagttaaa cggaattttg cagaggccaa actgttgatg
     2761 gtaaacaagg cttctaagaa tcgtgtcaaa ccagcttgtt ctgtttatga aacttgtggt
     2821 ggttgccaaa tcatgcatct agcctatcct aaacagctag atttcaaaga tgatgtgatt
     2881 aggcaggccc ttaaaaagtt caaaccaact ggctatgaac aatttgagat tagaccgact
     2941 ttaggaatga aaaagccaga tcactatcga gccaaattgc agtttcagct gcggtcattt
     3001 ggcgggacgg tcaaggctgg tttattttcc cagggaagtc atcgcttggt tcctattgac
     3061 aattgcttgg tacaggatca actaacgcaa gatattatca ataagattac gcagttagtg
     3121 gacaagtaca agttgcccat ttacaatgag cgcaagattg ctgggattcg gacgataatg
     3181 gttcgtaagg cacaggctag tgatcaggta cagattattg ttgtgagtag caaagaagtt
     3241 cggttagcga atttcattgg cgaattgact aaggcatttc ctcaggttaa aacggttgcc
     3301 ttgaatagta atcgttctaa atcgagtgag atttatgggg acgaaacgga aattctatgg
     3361 ggacaagagg cgattcatga agaagtttta gactatggat ttgcgttatc accgagagct
     3421 ttttaccaac ttaatccaca gcagacagaa gttttgtatg gtgaggtggt gaaggccttg
     3481 gatgtaggga gcaaagatca tattattgat gcctattgtg gggttggctc gattggcttt
     3541 gcttttgcag ggaaggtcaa gtctgtgcgg gggatggata ttatcccaga agcgattgaa
     3601 gatgcacaaa aaaatgccaa agccatgggc tttgacaacg cttactatga ggcagggaag
     3661 gcagaggata ttattcctaa atggtataaa caaggttacc gcgcggatgc catcattgtg
     3721 gacccaccaa gaactggttt agatgataaa ttattaaaaa cgattcttca ttaccagcct
     3781 aaacagatgg tgtatgtgtc ttgtaatacc tccactttgg cccgcgactt ggtgcagcta
     3841 acaaaggttt acgatgttca ctatatccaa agtgtagaca tgttccccca tacagctagg
     3901 accgaggctg tcgtgaagtt acaaaaacgt gtatgattga gtggcaataa caactcaagt
     3961 tttataagct caggtaatgg gtttgagcaa tagcttgaca tagtagggat gtcaaaaacg
     4021 gtttactaag catagtcata ttattgataa ctctatgttt attttagaaa cattcatggt
     4081 taaaaaagtt tgttagtttg tcactacgta gggtttattt gaagagcaag tacacacaga
     4141 caatattagg ggaattggtt gttgaaaaaa agctgttttt tgctataaat tagaaaactg
     4201 agtggtcagg aggttacatt accagaagtt attttagtat gctaatatga acgatgatat
     4261 agaggattta tcctgattta atcttttaag ctgtaagaaa cctttgcaaa aactgtaggc
     4321 tgtgtgagtc tttatgtgtt agatattaaa gataaatgca acacttgttt ataatgagat
     4381 caacaactga taaatctgag ttgataacca gtaaaatgat cggaatatag aaaagttact
     4441 taatcaagct tttcgtaatt taactgttag aatgacagaa cttatgataa aataaagaat
     4501 atgattaaaa tgaaaaagct cagtttatct attacggagt tcgaaatgaa aagaaaatcg
     4561 agatgtgata acatattttg aacgagaagg ag
//
LOCUS       DATXQK010000081         4456 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.081, whole genome shotgun sequence.
ACCESSION   DATXQK010000081 DATXQK010000000
VERSION     DATXQK010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4456
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.081"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            28..2301
                     /locus_tag="VC394_001657"
     CDS             28..2301
                     /locus_tag="VC394_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEP5995105.1"
                     /translation="MLEELKTLIKNPKLMITMIGVALVPALYNLSFLGSMWDPYGRVN
                     DLPIAVVNHDKPAKRADKSLTIGNDMVDKMSKSKDLEYHFVSAKQAQEGLKEGDYYMV
                     ITLPEDLSQRAATLLNPEPQKLTIRYQTSKGHGMVAAKMGETAMAKLKESVSQNITKT
                     YTSAVFSSMTDLQSGLKEASAGSQALASGAKTAQAGSQTLSTNLAALTGASQQFQQGT
                     GRLTSGLTTYTDGVNQVKNGLGTLSTDIPNYLNGVSRLSQGASQLNQGLSQLTQATTL
                     SDEKAKGIQSLIVGLPVLNQGIQQLNTELSTLQPPNLNADELGNSLGAIAQAAKQVIA
                     EETAAQNEQLSALQATSVYQSLTAEQQGELAAALSQSDKSQTVSAAQTILSSVQTLST
                     GLQTLSQGNSLAQLDQLKAAVSQIASQSNQALPGASSALSELSMGLSKVNGSLNQQVL
                     PGSNQLTTGLAQLNRYNTVIGSGVIKLSEGANALSSKSGELLDGSHQLSEGATKLADS
                     SSQLSQGGHQLTSGLTELSTGLSTLNGSLAKASQQLSLVSVTDKNAKAVAKPLVLNEK
                     DKDGVKTNGIGMAPYMIAVSLMVVALSTNVIFANSLSGRPVKDKWDWAKQKFVINGFI
                     STMGSIVLYLAIQLLGFEARYGMETLGFIMLSGWTFMALVTALVGWDDRYGSFASLVM
                     LLLQVGSSGGSYPIELSGAFFQKLHPFLPMTYVVSGLRQTISLSGHIGVEVKVLTGFL
                     LAFMVLALLIYRPKKTV"
     gene            complement(2418..3491)
                     /locus_tag="VC394_001658"
     CDS             complement(2418..3491)
                     /locus_tag="VC394_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HEP5995106.1"
                     /translation="MKNISRKCFMTSVVCIVLGAILLGAGYATGGLQDIKHQTAPKKV
                     IQTFDHVTALDIDTNFSTITVETGPVKKPTVTYYTHSKFIDPLEIKIENETLHISEKP
                     RDVIITGGIELIGFGLNKSDRNHNYRTTTITLPKGTSLKELKGSSMPQATISDLTVQD
                     LEFSGGLTLLNTRIKKGKVFGTFNATDSLLANLELKADYSLSSLTNSSLENGTISLGH
                     GQLTTKNTNLKSVTIQSLSPGGVETEETSLENVTFTVTSEQENEETNDNGAIFTAHAL
                     TLKGTNTISGGNVAVDITLTDPKTTAYKTKTEDGKILLGSQLTPAKIGKESTSDVISY
                     VAENKAATGNLTVNLNKGDITIK"
     gene            complement(3488..4075)
                     /locus_tag="VC394_001659"
     CDS             complement(3488..4075)
                     /locus_tag="VC394_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEP5995107.1"
                     /translation="MTRTEYLAELDKYLRKLPREDYHEALEYYIEYFDEAGPDKESQV
                     IDDLGNPKEAASEIISNVLGKHLATPEKTPKNRATIIGLTILSLFAAPIALPVLLALI
                     LFIIACLMLGLTAIFAAYIFGLAGILVAGVTLFESLTLIGSSIPAQAMGIGSALLCFG
                     GGLLIWIIATALLRFSGRAFVAFIKWISHKKGAQA"
     gene            complement(4062..4388)
                     /locus_tag="VC394_001660"
     CDS             complement(4062..4388)
                     /locus_tag="VC394_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEP5995108.1"
                     /translation="MYFPVSATLIEFLILAIVEKNDSYGYDISQTIKLVANIKESTLY
                     PILKKLEKAGFLTTYSQEHQGRKRKYYAVTSSGRAQLIFLKKEWQSYKFALDGIIEGS
                     LRHDKD"
BASE COUNT         1328 a          860 c          896 g         1372 t
ORIGIN      
        1 aacagtatgt ccattttagg agaaaatatg ttagaagaat tgaaaacact tattaaaaat
       61 ccaaaattaa tgattacaat gattggtgtg gccctagtgc ctgccttata taatttatcc
      121 tttctaggct caatgtggga tccttatggt cgggtcaatg accttcccat tgctgttgtt
      181 aatcatgata agcctgcaaa gagagctgat aagtcattga caattgggaa tgatatggtg
      241 gacaagatgt ctaaaagtaa agatttagag tatcattttg tctcagctaa gcaagctcaa
      301 gagggactta aagaaggtga ttattatatg gttattactt tacccgaaga tctttctcaa
      361 cgggcagcaa ccttattaaa tcccgaaccc caaaaattaa ctatccgtta ccaaacgagt
      421 aaaggacatg gaatggtcgc tgctaagatg ggggaaacag cgatggctaa gctgaaagag
      481 tctgtttcgc aaaacattac gaagacttat acctcagcag tttttagcag tatgacagac
      541 ctccaatcag gattaaaaga agcctcagct ggaagtcaag cacttgcttc aggagcgaag
      601 accgctcagg cggggagtca aacactttcg acgaacttag cagccttaac gggtgctagc
      661 caacagtttc aacaaggtac tggtcgattg acatcaggtt tgactaccta tacagatggt
      721 gtcaaccaag tcaagaatgg gttaggaaca ttatcaacgg acatccccaa ttatctgaac
      781 ggggtttcta ggttatccca gggagcttct cagcttaatc agggtctttc acagttgaca
      841 caagcaacaa cactttctga cgagaaagct aagggaattc aatccttaat tgtaggacta
      901 ccagtcctaa atcaaggcat tcagcaacta aatacagagc tatcaacatt gcaaccccct
      961 aaccttaatg ctgatgagtt aggtaatagc ttaggagcta tcgctcaagc tgccaaacaa
     1021 gttattgctg aagagactgc cgctcagaat gaacaactat cggctctcca agccactagt
     1081 gtttatcaat cattaactgc tgaacaacaa ggagagttag ctgcggccct cagtcaatct
     1141 gataaaagtc aaaccgtatc tgcagcccaa actattttaa gttctgttca aacactgtca
     1201 acaggtttac aaactctgtc ccaaggaaat agtttagctc aattagacca gcttaaagca
     1261 gctgtttcac agattgcgag tcagtctaac caggctttac ctggagcgag ttctgcatta
     1321 tctgagttgt caatggggtt atcaaaggta aatggtagct taaatcaaca agttctacca
     1381 ggaagtaatc aattgacaac aggattagca caattaaaca ggtataatac tgtcattggt
     1441 tctggggtaa taaaactctc agaaggtgcc aatgccttgt catccaagtc cggagaatta
     1501 ctagatggta gccatcaatt atcagagggt gctactaaac tagctgatag tagttctcaa
     1561 ttgagtcagg gtggtcatca attaacgagc ggattgactg aattatcaac aggattatca
     1621 accttaaatg gttccttagc caaagcctct cagcagttat cgcttgtttc tgtgactgat
     1681 aaaaatgcta aagctgtcgc aaaacctctt gtgttaaatg agaaagacaa agatggtgtt
     1741 aagacgaatg ggatcgggat ggcaccttat atgattgctg tttctctaat ggttgtggcc
     1801 ctttcaacca acgtcatttt tgctaattct ttatctggtc gtccggtcaa agataaatgg
     1861 gattgggcta aacaaaaatt tgttattaat ggttttattt cgactatggg atccattgtt
     1921 ctctacttag ctattcaatt attagggttt gaagcccgtt atggtatgga aaccttagga
     1981 tttattatgc taagtggttg gacgtttatg gctcttgtca cagctttggt cggttgggat
     2041 gatcgatatg gctcttttgc ttctttggtc atgttattac ttcaggttgg ctcttcaggt
     2101 ggctcttacc ccattgagtt aagtggagca ttcttccaaa agttacatcc tttcttacca
     2161 atgacttatg tggtatctgg tttacgacaa accatttcat tatcaggtca tattggagta
     2221 gaagtgaaag tcttaactgg tttcttactg gcatttatgg tattagcact actcatttat
     2281 cgtcccaaga aaacagtcta aataaaataa cctcaaaaaa aaacagcatc taaggcagtt
     2341 acactagatg ctgttttttg tcttattttg acaataactt gacaatttct gtcaagtttt
     2401 ttattgtctt gttaagatta tttgatagta atgtctccct tattgagatt aaccgttaaa
     2461 tttccagtag ctgctttatt ctcagccaca taagaaataa catctgaagt tgattcctta
     2521 ccaatcttag ctggtgtcag ctgtgatcca agtaagattt taccatcttc ggtttttgtc
     2581 ttataggcgg tcgtctttgg atcggtcaaa gttatgtcaa cagcaacatt accaccacta
     2641 atagtattag tgcctttaag ggtaagagca tgagcagtga atatagcacc attatcattg
     2701 gtttcctcgt tttcttgctc actagtaaca gtgaaagtca cattttcaag gcttgtttct
     2761 tcagtttcaa ccccaccagg agatagagat tggatggtga cagatttaag attagtattt
     2821 tttgtggtta attgtccatg ccctaagcta atcgtcccat tttctaaact agaattcgta
     2881 aggcttgaca acgaatagtc ggctttcaac tcaagattgg ctaacaagct atcagtagcg
     2941 ttaaatgttc caaaaacctt ccctttcttt attctagtat ttagaagtgt cagaccacca
     3001 ctgaactcaa ggtcttgaac cgttaaatcc gatatagtgg cttgaggcat gcttgagcct
     3061 ttgagctctt taagactggt accttttgga agcgttatgg tagtggttcg gtagttgtgg
     3121 ttgcggtcac ttttatttaa tccaaacccg atcagttcaa tacctccggt gatgatgaca
     3181 tctctgggtt tttcagaaat atgaagtgtc tcgttctcta ttttaatttc taaaggatca
     3241 ataaatttag aatgtgtgta ataggtaacg gttggttttt tgacaggccc tgtttctact
     3301 gtaatagttg aaaaattggt atcaatatca agagctgtta cgtgatcaaa tgtttggata
     3361 acctttttag gggctgtttg gtgtttaatg tcttgaagcc cgccagttgc ataaccagct
     3421 cctaaaagaa tggctcctaa aacaatacac acgacactgg tcataaaaca tttgcgtgag
     3481 atatttttca tgcttgagct cctttcttgt gactaatcca tttaataaag gcaacgaagg
     3541 cacgtcctga aaaacgcagg agtgcagtgg caataatcca aatgagaaga ccaccaccaa
     3601 aacacaacaa ggcgctgccg attcccatag cttgtgcagg aatagatgag ccaatcaagg
     3661 tcaagctttc aaacagggtc actcctgcga caagaatacc tgcaagccca aaaatgtagg
     3721 cggcaaagat agctgtgagc ccgagcatta ggcaggcaat aataaagaga atcaacgcca
     3781 gcaatacggg taaagcaatc ggagcagcaa agagggacaa aatggttaat ccaataattg
     3841 ttgcccgatt tttgggagtt ttctcgggag ttgccaagtg ttttcctaga acatttgaaa
     3901 tgatttcact ggcagcttcc ttaggatttc ctaaatcgtc gataacctgg ctttctttgt
     3961 caggaccagc ttcatcaaaa tattcaatat aatattccaa cgcttcatgg tagtcttctc
     4021 taggtagttt gcgcaagtat ttatctagtt ctgctagata ctcagtcctt gtcatgtctt
     4081 aagctccctt caataatccc gtctaaggca aatttatagc tctgccattc tttttttaga
     4141 aaaatcagct gtgctctccc cgaagaagtg acagcataat atttacgctt tcgtccctga
     4201 tgttcttgac tataagttgt caaaaaacct gccttctcca attttttaag aataggatag
     4261 agggtggatt ctttgatatt agccactagt ttaatggttt gactaatatc gtaaccgtaa
     4321 gaatcgtttt tttcgacaat ggctaaaatc agaaattcaa tcaaggtagc cgatactgga
     4381 aaatacattt taggctcctt tcatatataa aaattttata tataattatt ttacatattt
     4441 agtataagct tagttt
//
LOCUS       DATXQK010000082         4396 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.082, whole genome shotgun sequence.
ACCESSION   DATXQK010000082 DATXQK010000000
VERSION     DATXQK010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.082"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..103)
                     /locus_tag="VC394_001661"
     CDS             complement(<1..103)
                     /locus_tag="VC394_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HEP5995109.1"
                     /translation="MDEFKGIKVEFIQLSNKNIFDVLEKDLSKKLRFE"
     gene            complement(89..214)
                     /locus_tag="VC394_001662"
                     /pseudo
     CDS             complement(89..214)
                     /locus_tag="VC394_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
     gene            complement(319..591)
                     /locus_tag="VC394_001663"
                     /pseudo
     CDS             complement(319..591)
                     /locus_tag="VC394_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424370.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(610..915)
                     /locus_tag="VC394_001664"
     CDS             complement(610..915)
                     /locus_tag="VC394_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEP5995110.1"
                     /translation="MKEYQVTMSDDAKADLLSIYHYVRDELCAPQAADNLLERLSQEM
                     LSLSIFPERCAIIEDLIGKGYTFRQLIVKKYRIVYHVLEDEVIIVAVVYGSRHMNNW"
     gene            complement(915..1217)
                     /locus_tag="VC394_001665"
     CDS             complement(915..1217)
                     /locus_tag="VC394_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEP5995111.1"
                     /translation="MAKTGTLNLRVDDSVKSAADDILKRLGIPMSTAIDMFLNQIILT
                     GGIPFDVSLPEAPQRVNVDYMSQEKFYDKLIFSFEDAKTGNRKDVREFLSQLKENA"
     gene            complement(1272..1448)
                     /locus_tag="VC394_001666"
     CDS             complement(1272..1448)
                     /locus_tag="VC394_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HEP5995112.1"
                     /translation="MGQNVVMRQAPGGNIKPDKEKSVEKIDGIVGLILGLDRCIRRQG
                     DEASVYDERGILSF"
     gene            1464..2357
                     /locus_tag="VC394_001667"
     CDS             1464..2357
                     /locus_tag="VC394_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5995113.1"
                     /translation="MTTMLDFPFHHEFIEFLGRWTHILITLLKRHHGQADTAIPRYLL
                     VTIREKTGIVKSTTVQNTAFTTQSNQLKKQYGQFLLTESNNCCACPGCDRPLILTSGN
                     LAAENYEVSAIEKDKDAEPLNLIALCPDCFLTYQADSRKKIVTALKNVKKILVSAHNS
                     QQAISDMKLDSGIVSVLTSLNKLKCDEYDISYDSKQLTDKISPENNRTLYQLVKNPVI
                     DNYLTIQKIIVNLDKQGKIDYEEIQYQTRSMYKKLKAAKHNNLAIFNTISEKLHKATL
                     QDIYFCQTIVSYFIQKCEVLE"
     gene            2357..2581
                     /locus_tag="VC394_001668"
     CDS             2357..2581
                     /locus_tag="VC394_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5995114.1"
                     /translation="MQLPNKLYSYQKSTLAYLPRVLNEIKSGNSNVKDIFHAISEELD
                     DPTDFLSIMDCLYALNAIEMSNEGEVKLCL"
     gene            2572..4230
                     /locus_tag="VC394_001669"
     CDS             2572..4230
                     /locus_tag="VC394_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2326 domain-containing protein"
                     /protein_id="HEP5995115.1"
                     /translation="MLIEMWSPVFKNHGEIRESICFHYGLNVIMGMDLADNSIGKSSS
                     LLAIDFIFGGNSYLKSIAVKKLGDHPIYFCFQFEKKFYFSRDTANPDIILVCDKSYSP
                     TGETMDLGIFLNKLKKRYHLDSPELSFRLAMSGFFRIAGKSNQNTDFPLQVYSSQKSS
                     ESITTLIQLFNLYDNIARYKERLKDKSDQLTTFRNARKYTFISNLVGGKKQFEANVSE
                     IKRLEYDLAHLQDTHQDKIDSDDIEKNQQKLQLRNTKLELESSLRDKQRRLKLLDISI
                     EFGLYPTESDLTELQQYFPDTNLKKLYEVEAYHKKLETILDSEFSTERESLIAEIDEL
                     ESQLTTLNQELQELGNIPNLSSEYLESYSKLTATINALKEQNEAYLKESGLSKEKSEA
                     DADLKRSTEDILLDLEVKINSKMREFNNILYPDIRKAPQINLNAHNSYSFYTPDDDGT
                     GTKFKGMIVFDLVMLYLTNLPALTHDSLLLSNVSYQATEALLKLYDQSRSLNKQVFLA
                     FDKASSYSPDANQLLSENTVLRLSSNGNELYGISWNKGENSDEI"
     gene            4220..>4396
                     /locus_tag="VC394_001670"
     CDS             4220..>4396
                     /locus_tag="VC394_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEP5995116.1"
                     /translation="MRFSYNKLWKLLIDKGWTKTKLRQEAGISSSTIAKLGKGENITT
                     DILLEICIALDCKIE"
BASE COUNT         1473 a          902 c          728 g         1293 t
ORIGIN      
        1 cttcaaatct taacttttta gataaatctt tctccaaaac atcaaatata tttttgttag
       61 acagttgaat aaattctact ttaattcctt taaactcatc cacattctcg ccactagtcg
      121 tgcgaccaaa gaggtagaaa aagtcctgac cttcataact gttttgaggc ttatctctag
      181 ccctttttct tccaattaaa tcaagtagtc ccataagccc tccttatttt ggggtacaaa
      241 aaaagcacct cctttgaagt gctttcggta tatttctttc aaagtaatca acagtctttc
      301 ttacaaaacc tagtattctc attctgtcaa ttccttaaat gcgtcaagat gtcgtgataa
      361 tactgaagtc gcaatctcat ctaaccttgc ttgttcaaca actgttgtca tctgctcatc
      421 actgcttaaa ctttggtgat ccgctgatag tttgctatca gatggactta ataccaaagt
      481 tccatcagtt gatttataaa ggttaaactc ctaattctct tgaatgccaa atgggtcagg
      541 tatcggaaga tagatgtcat caccaatttg tatcgtcttt acgagttcca tacatcactc
      601 tcccttactt taccaattat tcatatgacg tgatccatag acgaccgcaa caattataac
      661 ttcatcttcc agaacatggt atacaatgcg atattttttg acaatcagtt gtctgaaggt
      721 atagcctttt ccaatcagat cctcaatgat ggcacaacgt tcaggaaaaa tggatagtga
      781 taacatttct tgggataacc tctcaagaag attatccgct gcctgtggtg cacagagttc
      841 atcacgaaca tagtgataga tgctcagcaa atctgcttta gcatcatccg acatggtgac
      901 ctgatattct ttcattaagc attttccttc aattgagata gaaattctct gacatccttg
      961 cggttgccag ttttcgcatc ttcaaagctg aagataagct tatcataaaa cttctcctga
     1021 ctcatgtaat caacattaac tcgttgaggc gcttcaggta gagaaacatc aaatgggata
     1081 cccccagtca aaataatctg attcaaaaac atatcaatcg cagttgacat aggaatacct
     1141 aagcgtttca aaatatcatc tgccgcactt tttactgaat catcaactcg taaattcaaa
     1201 gttcccgttt tagccatggt tatcctcctt ttttgtaacg acattgtatc acgtttttag
     1261 agttgactca actaaaaact caatatccct cgctcatcat agacgctagc ttcatcacct
     1321 tggcggcgaa tgcaacggtc aagccccagg ataagaccta caataccgtc aatcttctca
     1381 acagattttt ccttatctgg cttgatgttg cctccagggg cttgtcgcat caccacgttt
     1441 tgtcccatcc atttaaggac tggatgacca ccatgttaga ttttcccttc catcatgagt
     1501 ttatagagtt ccttggacgg tggactcata tccttataac tctgctcaaa aggcaccatg
     1561 gtcaagccga caccgcaatt ccaaggtatc ttctagtaac cataagagag aagacaggga
     1621 ttgtaaaatc aaccactgtg caaaatactg cgttcactac ccagtcgaat cagctcaaaa
     1681 aacaatacgg gcagttcttg ttaacagaat ctaataactg ttgtgcatgc ccaggctgtg
     1741 atagaccact cattctaacc agtggcaact tagcagcaga aaattacgag gtttcagcca
     1801 ttgaaaaaga taaggacgct gaaccactga atctgatagc cctctgccca gattgttttt
     1861 taacctatca agcagatagt cgcaaaaaaa ttgtaacagc tctaaaaaat gttaaaaaga
     1921 ttctagtctc tgcacataac agccaacaag cgatttctga tatgaagtta gacagtggaa
     1981 ttgtttcagt tttgactagt ctcaataaac taaaatgtga tgaatatgat atctcctatg
     2041 actcgaagca attgactgat aagatatcac ctgagaacaa tcgtacgctt tatcaattgg
     2101 ttaaaaaccc agttattgat aactacttaa ccattcaaaa aatcatcgta aatttggaca
     2161 aacaaggaaa aattgattac gaggaaatcc aatatcaaac gcgatcgatg tataaaaagt
     2221 taaaagcagc caagcataat aatctggcta ttttcaatac tatttcggaa aaactccata
     2281 aagctacact acaggacatc tacttctgtc agacgattgt ctcttacttt atccaaaaat
     2341 gcgaggtact tgaataatgc aattaccaaa taaactttac tcctatcaaa aaagcaccct
     2401 tgcttatttg cctagagtgc taaatgaaat caaaagtgga aactcaaacg tcaaagatat
     2461 ctttcacgct atctcagaag agttggatga tcctacagat ttcttatcta taatggattg
     2521 cttgtatgca ttgaacgcta ttgaaatgtc taatgaagga gaagtaaaat tatgcttata
     2581 gaaatgtggt ctccagtatt taagaatcac ggagaaattc gggaatcaat ctgcttccac
     2641 tatgggttaa acgtgattat ggggatggat ttagctgata actctatcgg caaatcctcc
     2701 tcactattag ccattgattt catctttgga ggtaatagct atctaaaatc aattgcggtt
     2761 aaaaaattag gtgatcatcc tatctatttc tgctttcaat ttgagaaaaa gttttatttt
     2821 tcaagagata cggccaaccc agatatcatt ctagtgtgtg acaagagcta ctctccaacc
     2881 ggtgaaacaa tggacctagg aatttttctt aacaaactta agaaacgata tcatttagat
     2941 tctccagagc tatcattccg attagctatg agtggcttct ttaggattgc aggaaaaagt
     3001 aatcaaaata ccgatttccc tttacaagtt tattcgagcc aaaaatcttc tgaatctatt
     3061 actactctca tccaactgtt taatttgtat gataacattg cacgatacaa agaacgtctg
     3121 aaagataaga gcgaccagtt aacaactttt cgtaatgctc gaaagtatac cttcatctcc
     3181 aacctggttg gcggtaaaaa gcaatttgaa gcaaatgtct cagaaataaa aagattggaa
     3241 tatgatttag cacatctcca agacactcat caagataaaa ttgactctga tgatattgag
     3301 aaaaatcaac aaaaactcca gcttagaaat actaagctgg agttagaaag cagtcttcgg
     3361 gacaagcaaa gacgtcttaa gctattggac atcagtatcg aatttggctt atatcctact
     3421 gaatctgact taacagagct tcaacaatac ttcccagata ctaatctcaa aaaattatat
     3481 gaagttgaag cttaccacaa aaaattggaa actattttag attccgaatt ctcaactgaa
     3541 cgtgaatccc taatagctga gatcgacgaa ttagagagtc aattaacaac tctaaaccaa
     3601 gaacttcaag aactaggaaa catcccaaat ctttcaagtg agtacttaga aagctactct
     3661 aaactaacag ctacaatcaa tgcactaaaa gaacaaaacg aggcctactt aaaggagtct
     3721 ggtctatcca aagaaaaatc agaagcggac gctgacttga aacgcagtac agaagatatc
     3781 cttctagatc tagaagtaaa aatcaacagt aagatgcgtg aattcaataa cattctatat
     3841 ccagatattc gcaaagctcc tcaaattaac ctcaatgctc acaatagcta ctccttctat
     3901 acaccagatg atgatggtac aggaactaag tttaaaggga tgattgtatt cgacttagta
     3961 atgctctacc ttactaatct gccagcactc acacacgatt cattactctt gagtaatgtt
     4021 agctaccaag cgactgaagc acttttgaag ctgtatgacc agtcaaggtc attgaacaaa
     4081 caagtctttt tagccttcga taaagccagc tcttattctc cagatgctaa tcaactcttg
     4141 tcagaaaata cggtattgag actctctagt aacggaaatg aactctacgg tatttcatgg
     4201 aacaaaggag aaaactcaga tgagatttag ttacaataaa ttatggaaac ttcttattga
     4261 taaaggttgg accaaaacaa agctcaggca agaagctggg attagttctt ccacgatcgc
     4321 aaaattggga aaaggggaaa atatcactac tgatatttta ttggaaattt gtattgcact
     4381 tgattgtaag attgaa
//
LOCUS       DATXQK010000083         4242 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.083, whole genome shotgun sequence.
ACCESSION   DATXQK010000083 DATXQK010000000
VERSION     DATXQK010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.083"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..153
                     /locus_tag="VC394_001671"
     CDS             <1..153
                     /locus_tag="VC394_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HEP5995117.1"
                     /translation="TTFDINVENLGKMSFKQLLDIINSNEQNLSQTIFVPFSLKQRES
                     VRDISH"
     gene            269..1762
                     /gene="malQ"
                     /locus_tag="VC394_001672"
     CDS             269..1762
                     /gene="malQ"
                     /locus_tag="VC394_001672"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEP5995118.1"
                     /translation="MNKRASGILMHISSLPGKFGIGTFGKSAFEFVDFLAETKQTYWQ
                     ILPLTTTSFGDSPYQSFSAIAGNTHFIDFELLVDDELLEAADLCDITFGTNPEAVDYA
                     QLFQVRRPLLEKAVRAFVAEQENVCKLEAFETASSWLTDFAEFMALKEYFNNKALQDW
                     DDETVIKRQEDSLNNYRELLAKKITYHKVCQYFFYQQWSALKTYANHKGIEIIGDMPI
                     YVSADSVEVWTMPELFKVDSDKKPLFIAGVPADGFSEDGQLWGNPTYNWSAHEKSNFA
                     WWIYRIQESFKLYDQLRIDHFKGFSDFWEIPAGDKTARNGHWASAPGIALFSAVREAL
                     GELPIIAENLGYIDEKAEQLLASTGFPGMKILEFGLFDITSQSIDLPHYYDRNCVAYT
                     GTHDNEVVNGWYDNLSEEQVHFVNNYLHKHADESITKAMLRTIFASVCDTAILCIQDL
                     LDKDGKSRMNMPNTIGGNWQWRMLDGELNQDHKDYLIYLTDLYGRAR"
     gene            1797..4061
                     /gene="glgP"
                     /locus_tag="VC394_001673"
     CDS             1797..4061
                     /gene="glgP"
                     /locus_tag="VC394_001673"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEP5995119.1"
                     /translation="MTRFTEYVETKLGKSLTQASNEEIYLSLLNFVKEEASHKAKNTA
                     KRKVYYISAEFLIGKLLSNNLINLGIYKDIKEELAAAGKSIAEVEDVELEPSLGNGGL
                     GRLASCFIDSISSLGINGEGVGLNYHCGLFKQVFKHNEQEAEPNFWIEDDSWLVPTDI
                     SYDVPFKNFTLKSRLDRIDVLGYKRDTKNYLNLFDIEGVDYGLIKDGISFDKTQIAKN
                     LTLFLYPDDSDKNGELLRIYQQYFMVSNAAQLIIDEAIERGSNLHDLADYAYVQINDT
                     HPSMVIPELIRLLTEKHGFDFDEAVAVVKNMVGYTNHTILAEALEKWPTAYLNEVVPH
                     LVTIIEKLDALVRSEVSDPAVQIIDESGRVHMAHMDIHFATSVNGVAALHTEILKNSE
                     LKAFYDLYPEKFNNKTNGITFRRWLEFANQDLADYIKELIGDEYLTDATKLEKLMAFA
                     DDKAVHAKLAEIKFNNKLALKRYLKDNKGIELDEHSIIDTQIKRFHEYKRQQMNALYV
                     IHKYLEIKKGNLPKRKITVIFGGKAAPAYIIAQDIIHLILCLSELINNDPEVSPYLNV
                     HLVENYNVTVAEHLIPATDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIA
                     ELAGMENIYTFGKDSDTIINLYATASYVAKDYYDNHPAIKAAVNFIISPELLAFGNEE
                     RLDRLYKELISKDWFMTLIDLEEYIEVKEKMLADYEDQDLWMTKVVHNIAKAGFFSSD
                     RTIEQYNEDIWHSR"
BASE COUNT         1401 a          743 c          815 g         1283 t
ORIGIN      
        1 acgacatttg atataaatgt tgaaaatctt ggaaaaatgt cttttaagca attacttgat
       61 attatcaatt ccaatgaaca gaatcttagt cagactattt ttgttccttt tagtttaaaa
      121 caaagagaaa gcgtccgtga tattagtcat taagagcaaa aaaaagtagc atttttaagg
      181 aatgttactt tttttaggaa actggttgac ttacgcaagc gcttgcgtta taatctcatc
      241 atgaatagat ttcacaagga gaaaaatcat gaacaaacgt gcaagcggta tcttaatgca
      301 tattagttcc ctgcctggga aatttgggat aggcactttt ggaaaatcag cttttgaatt
      361 tgttgatttt ctagcagaga cgaaacaaac ctattggcaa attctgcctt taacaacgac
      421 gagttttgga gattctcctt atcagtcatt ttcagctatt gctgggaaca cacatttcat
      481 tgattttgaa ttgctagtag atgatgaact tttagaagca gcagatttgt gtgatattac
      541 attcggcaca aatcctgaag cagtagacta tgctcagctt tttcaagtta ggcgtccact
      601 tttagaaaaa gcagtgagag cttttgttgc tgaacaagaa aatgtgtgta aattagaagc
      661 ttttgaaaca gcttctagct ggttaactga ttttgctgaa tttatggctt taaaagaata
      721 ttttaacaat aaagccttac aagattggga cgacgaaact gtcattaaac gccaagaaga
      781 ctcacttaat aattaccgtg agttgcttgc aaaaaagatt acctatcata aagtctgtca
      841 atacttcttt taccaacaat ggtcagcctt gaaaacttac gctaaccata aagggataga
      901 aattattggt gatatgccta tttatgtttc tgcggacagt gtagaagttt ggacgatgcc
      961 tgagctattt aaagtagata gtgataaaaa gccattgttt attgcaggtg taccagcaga
     1021 tggctttagt gaagatggtc agctgtgggg aaatccaacc tacaattggt ctgctcatga
     1081 aaagtctaat tttgcatggt ggatttatcg tattcaagaa agtttcaaac tatatgatca
     1141 gctaagaatc gatcatttta aaggcttctc agatttttgg gaaattcctg ctggtgataa
     1201 aactgctaga aatggccatt gggcctcagc cccaggtata gcattatttt ctgcggttcg
     1261 tgaggcctta ggagagctcc ctatcatcgc agaaaacctt ggttatattg atgaaaaagc
     1321 agagcagcta ttagcatcaa caggctttcc tggcatgaag atattagaat ttggtttatt
     1381 tgatataacg agtcaaagta tagatttacc acattactat gaccgtaatt gtgtcgctta
     1441 cacagggact catgataacg aggtggttaa cggatggtat gacaatctta gtgaagaaca
     1501 agtacacttt gtaaataact atcttcacaa acatgcagat gaaagtatta caaaagccat
     1561 gctaagaaca atctttgctt ctgtttgtga tacagccatt ctttgcatcc aagatttatt
     1621 agataaagat ggtaagagtc gtatgaatat gccaaataca attggaggta attggcaatg
     1681 gcgtatgctt gacggtgaat tgaaccaaga tcacaaagat taccttattt atttaacaga
     1741 tctttatggt cgcgcaagat aatatttaaa tgttacatta ttgaaaagag gaagacatga
     1801 cacgctttac agaatatgtt gaaactaaac taggaaaatc acttacgcag gctagcaatg
     1861 aagagattta tctatcatta ttaaactttg tcaaagaaga agctagccac aaagctaaaa
     1921 atactgccaa acgcaaagtt tactatatct cagcggaatt tttgattggt aaattactat
     1981 caaacaacct gattaaccta ggaatttata aagatatcaa agaagaattg gcagctgctg
     2041 gcaaatctat cgcagaagtc gaagatgttg aattagaacc atcactaggt aatggtggtt
     2101 taggacgcct tgcttcatgt ttcattgact ctatttcatc tcttgggatt aatggtgaag
     2161 gggttggttt aaactatcat tgtgggttgt ttaagcaagt cttcaaacac aatgagcaag
     2221 aagctgagcc aaacttctgg attgaagatg actcatggtt ggttccaaca gacatttctt
     2281 acgatgtgcc ttttaagaac ttcaccttga aatctcgtct tgatcgtatt gatgttttgg
     2341 gttacaaacg cgacactaaa aactacctta acttgtttga tatcgagggc gttgattacg
     2401 ggttaatcaa agacggtatt tcatttgata aaacgcaaat tgctaaaaac ttgaccttgt
     2461 tcctctaccc agacgattct gacaaaaatg gggaattgct ccgtatttac caacagtact
     2521 ttatggtgtc aaatgcagcg caattaatca ttgatgaagc tatcgaacgt ggttcaaacc
     2581 ttcatgacct tgcagactac gcttacgtgc aaatcaatga cacgcatcca tcaatggtca
     2641 tcccagaatt aattcgtctc ttgactgaaa aacatggctt tgactttgat gaagcggtag
     2701 ctgttgtgaa aaatatggtt ggttacacta accacactat tcttgcagaa gcccttgaaa
     2761 aatggccaac tgcttactta aacgaagtag tgccacactt ggtaaccatc attgaaaaat
     2821 tggatgctct tgttcgttca gaagtgtctg atccagctgt tcaaattatt gacgaatcag
     2881 gtcgtgtgca catggcccat atggatattc attttgcaac aagtgtcaat ggggtagcag
     2941 cactccacac agaaatcttg aaaaacagcg aattaaaagc tttctatgac ctttacccag
     3001 aaaaattcaa caacaaaacc aacgggatta ctttccgtcg ttggctagaa tttgctaacc
     3061 aagacttggc tgattacatt aaagaactta ttggcgatga gtacttgact gacgcaacaa
     3121 aattagaaaa attgatggcc tttgcagatg acaaagctgt tcatgctaaa ttggctgaaa
     3181 tcaaattcaa caacaaatta gctcttaaac gttaccttaa agacaataaa ggcattgagc
     3241 ttgatgaaca ttctattatt gatacccaaa tcaaacgttt ccacgagtac aaacgtcaac
     3301 agatgaatgc tctttacgtg attcacaaat atttggaaat taaaaaaggc aaccttccaa
     3361 aacgtaaaat cactgttatc tttggaggta aagcagcgcc tgcttacatc attgctcaag
     3421 acatcattca cttgatcctt tgcttgtctg aattgattaa caatgaccct gaagtaagcc
     3481 cataccttaa tgtgcatcta gttgaaaatt acaacgtgac agtagcagag cacttgattc
     3541 cagcaactga tatttctgag caaatttcac tagcatccaa agaagcttct ggaactggta
     3601 atatgaaatt catgcttaac ggtgctttaa cacttggtac aatggacggt gctaacgtag
     3661 aaatcgctga gcttgcaggc atggagaata tctacacctt tggtaaagat tctgacacca
     3721 tcatcaacct ttatgcgact gcttcttatg tagcaaaaga ttactatgat aaccatcctg
     3781 ctattaaagc agcagtgaac tttattatca gtccagaatt gctagcattt ggcaacgaag
     3841 aacgtcttga tcgtctttat aaagaattga tttcaaaaga ctggttcatg actttgattg
     3901 accttgaaga gtacattgaa gtgaaagaaa aaatgttagc agactatgaa gaccaagatt
     3961 tatggatgac aaaagtggtc cataacatcg caaaagctgg attcttctca tctgaccgta
     4021 ccattgagca gtataacgaa gatatttggc attcacgata aaaaataata attgcaatgt
     4081 ggggtagcgg taccgtaaca tgcttaaact aaatcataag acagtagttt ctgctgtctt
     4141 atgatttttc ttcagactta gattaattat tacattaagc gtaaaacaag tctgtttatc
     4201 gatctatgaa ggcaattata agaaattaca ctaaagtttt tt
//
LOCUS       DATXQK010000084         4203 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.084, whole genome shotgun sequence.
ACCESSION   DATXQK010000084 DATXQK010000000
VERSION     DATXQK010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.084"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            10..1035
                     /locus_tag="VC394_001674"
     CDS             10..1035
                     /locus_tag="VC394_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEP5995120.1"
                     /translation="MSEKSLGEVLRESRVGKNITLDDIELKTGISSHYLLAMELDQFK
                     IIPEEKFDQFLKEYADIVELDFNTLKRRYRYQVNSKKDSDIKSVTEIVEEKLSKKRLQ
                     EKLLSHQSMGYGQPLEVSKNKETLVIPVKTVGLDSKSPLKTISPVSQFKSDNRLIPKK
                     NVSRMRRNSTDSRSKTSLFPIAILGTVAFAIISVIILGVWQQFEKSQRAKEAEVALLK
                     ASEQSTNGLKNNSNDRKTQVTTEGSGNYLIATVTKSKETVDISVSLTEAQSSWISLTN
                     SEIGEGGITLTQDSPTYTATLPAEITESLLTLGVTKGVSVTIDGQPVDLSPLTSTDLS
                     YITFKIQ"
     gene            1049..1591
                     /gene="pgsA"
                     /locus_tag="VC394_001675"
     CDS             1049..1591
                     /gene="pgsA"
                     /locus_tag="VC394_001675"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEP5995121.1"
                     /translation="MIKKENIPNLLTLVRIAMIPFFLFITSSSNKVGWHIFAAVIFAI
                     ASFTDYLDGYLARKWHVASNFGKFADPLADKMLVMSAFIMLVGLGLVPAWVSAVIICR
                     ELAVTGLRLLLVETGGKVLAAAMPGKIKTATQMLSIILLLCHWIFLGNVLLYIALFFT
                     IYSGYDYFKGASFLFKDTFK"
     gene            1593..2432
                     /locus_tag="VC394_001676"
     CDS             1593..2432
                     /locus_tag="VC394_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEP5995122.1"
                     /translation="MSAIIELKKVTFNYHKDQEKPTLDGVSFHVKQGEWLSIIGHNGS
                     GKSTTIRLIDGLLEPESGSIIVDGDLLTITNVWEIRHKIGMVFQNPDNQFVGATVEDD
                     VAFGLENKGIAHEDIKERVNHALELVGMQNFKEKEPARLSGGQKQRVAIAGAVAMKPK
                     IIILDEATSMLDPKGRLELIKTIKNIRDDYQLTVISITHDLDEVALSDRVLVMKDGQV
                     ESTSTPEQLFARGDELLQLGLDIPFTTSVVQMLQEEGYPVDYGYLTEKELENQLCQLI
                     SKM"
     gene            2408..3250
                     /locus_tag="VC394_001677"
     CDS             2408..3250
                     /locus_tag="VC394_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEP5995123.1"
                     /translation="MSINLQNVSYTYQAGTPFEGRALFNINLDILDGSYTAFIGHTGS
                     GKSTIMQLLNGLHVPTTGIVSVDKQDITNHSKNKEIKSIRKHVGLVFQFPESQLFEET
                     VLKDVAFGPQNFGVSPEEAEALAREKLALVGISENLFEKNPFELSGGQMRRVAIAGIL
                     AMQPKVLVLDEPTAGLDPKGRKELMTIFKKLHQSGMTIVLVTHLMDDVANYADFVYVL
                     DKGKIILSGKPKTIFQQVSLLEKKQLGVPKVTKLAQRLVDRGIPISSLPITLEELKEV
                     LKHG"
     gene            3243..4043
                     /locus_tag="VC394_001678"
     CDS             3243..4043
                     /locus_tag="VC394_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEP5995124.1"
                     /translation="MDKLILGRYIPGDSLIHRLDPRSKLLAMIIYIVIIFWANNVVTN
                     LLMLTFTLAVVFLSKIKLSFFLNGVKPMIGIILFTTLFQMFFSQGGKVIFSWWFISIT
                     DLGLSQAILIFMRFVLIIFFSTLLTLTTTPLSLSDAVESLLKPLTRFKVPAHEIGLML
                     SLSLRFVPTLMDDTTRIMNAQRARGVDFGEGNLIQKVKSIIPILIPLFASSFKRADAL
                     AIAMEARGYQGGEGRTKYRQLDWQLKDSLAIGIVSLLGLLLFFLKTSL"
BASE COUNT         1340 a          668 c          818 g         1377 t
ORIGIN      
        1 ggtacctgta tgagtgaaaa aagtcttggt gaagtgttaa gagaatcgcg tgttggaaaa
       61 aatatcacac tagatgatat agaattgaag acagggattt cttcccatta cttgctggca
      121 atggaattgg atcagtttaa aattattccg gaagaaaaat ttgatcaatt tttaaaagaa
      181 tatgctgaca ttgttgagtt agatttcaat actttaaaaa gacgataccg ttatcaagtt
      241 aattccaaaa aagacagtga tataaaatct gtaactgaga ttgttgaaga gaagttaagt
      301 aagaaacgat tacaagagaa gttgttatca catcagagca tggggtatgg tcaaccgtta
      361 gaagtttcta aaaacaaaga aaccttagtt atccctgtaa agactgttgg tttagatagc
      421 aaatcgccac taaaaacgat atcgcctgtt agccaattta aatcagataa caggttaatt
      481 cctaaaaaaa atgtgtcacg tatgaggcga aatagcactg acagtcgttc taaaacgtca
      541 ctatttccca tagctatatt aggaacggtt gcttttgcaa ttatcagtgt tatcatttta
      601 ggtgtttggc aacaattcga aaagagtcaa agagctaaag aagctgaagt tgctttgctt
      661 aaagcctctg agcagtctac taacggttta aaaaataatt caaatgatcg caaaactcaa
      721 gtcacaactg aggggtcggg taattattta atagcgactg ttactaaatc taaagagacc
      781 gttgatatca gtgtgtcttt aactgaggca caaagtagtt ggatttctct aacaaattca
      841 gaaattggtg aaggtggtat aacattgact caagactcgc caacttatac agcgacttta
      901 cctgcagaaa taacagaatc attgttgaca cttggtgtga caaaaggtgt atctgtaaca
      961 attgatggac aacctgttga tctatcacct ttaacaagta ctgacttaag ttatatcacg
     1021 tttaagatcc aataattaag gtagactaat gataaaaaaa gaaaatattc ctaatctatt
     1081 aactcttgtt cgaatcgcaa tgattccttt ctttcttttt attacttcct catctaataa
     1141 ggtggggtgg catatttttg cagctgtgat ttttgcaatt gccagtttta ccgattatct
     1201 agatgggtat cttgcgcgta agtggcatgt agccagtaat tttggaaagt ttgccgatcc
     1261 tctagcagat aagatgcttg tcatgagtgc ctttatcatg ttagttggac ttggcttagt
     1321 tcctgcgtgg gtgtcagctg ttattatttg ccgagaattg gcagtaactg gtcttcgatt
     1381 actacttgtt gaaaccggag gaaaggtcct tgcagctgct atgccgggaa aaatcaaaac
     1441 agcgacacaa atgttgtcta ttattttatt actttgccat tggatattcc taggaaacgt
     1501 cttactctat attgctcttt ttttcactat ttattctgga tatgattatt ttaaaggagc
     1561 aagctttctt tttaaggata cgtttaaata acatgtcagc tattattgaa cttaaaaaag
     1621 ttacatttaa ttaccataaa gaccaagaaa aaccaacatt agatggcgta tcgtttcatg
     1681 tgaaacaagg tgagtggctg tctatcattg gccataacgg atctggtaaa tcaacgacta
     1741 ttcgattaat tgatggatta ttggaaccag aatcagggtc tattattgtt gatggagatc
     1801 tactaaccat aactaatgtt tgggaaattc gtcataaaat tggtatggtc tttcaaaacc
     1861 ctgataatca gtttgttgga gcaactgttg aagatgatgt tgcttttggg cttgaaaata
     1921 aaggtattgc acatgaagat ataaaagaga gggttaatca tgctttagag ttagtcggca
     1981 tgcagaactt taaagaaaaa gaaccagccc gtttatctgg tggccaaaaa cagcgcgtag
     2041 ctattgcagg tgcagttgct atgaagccta aaattattat tttagatgaa gctactagta
     2101 tgcttgaccc taaaggacga ttagagttaa tcaaaactat aaaaaacatc cgtgacgact
     2161 accagctgac tgttatttcc attactcatg acttagatga agttgctctt agtgatagag
     2221 ttttagtgat gaaagatggt caagtggaat caacatcaac accagaacaa ttatttgcaa
     2281 gaggggatga actactccaa ttaggtcttg atatcccttt tacaacatct gttgtacaga
     2341 tgcttcaaga agaaggttat cctgttgact atggatatct cacagaaaag gaattagaaa
     2401 atcagttatg tcaattaatc tccaaaatgt aagttatact tatcaagcag ggactccttt
     2461 tgaagggcgt gcccttttta acattaattt agatatttta gatggttctt atacggcttt
     2521 tatcggacat actggatccg ggaaatcgac cattatgcaa ttgctaaatg gtttacacgt
     2581 tccgacaacg ggaattgtca gcgtagataa acaagatata acgaatcact ctaaaaataa
     2641 agagattaaa tctattcgaa aacatgtagg attagttttt caatttccag aaagtcaact
     2701 atttgaagag acagttttga aagatgttgc ttttggccct caaaattttg gggtctctcc
     2761 ggaggaagca gaagctttag cgcgtgaaaa actagctctt gttgggattt cagaaaatct
     2821 ttttgaaaag aatccatttg aactctcagg gggtcaaatg cgtcgtgttg ctattgcggg
     2881 tatccttgct atgcagccaa aggttttagt tttagatgaa ccgactgctg gtcttgatcc
     2941 taaagggcgt aaagaattaa tgactatttt taaaaagtta caccaatctg gcatgactat
     3001 tgttttagta acccatctga tggatgatgt ggccaattac gcagattttg tatatgttct
     3061 tgacaaaggt aaaattattt tatcaggaaa acccaaaaca atttttcaac aagtcagttt
     3121 gcttgagaaa aaacagttag gagttcctaa ggtgactaaa cttgctcaaa ggcttgttga
     3181 tagaggaata cctatctctt ctttgcctat taccttagag gagctaaaag aggtactaaa
     3241 acatggataa gctgattctt ggtcgttata ttcccggaga ttctctcatt catcgtcttg
     3301 acccaagaag taaattatta gcgatgatta tttatattgt cattatcttt tgggctaaca
     3361 atgttgtgac aaatttgttg atgttgactt ttactttagc agttgttttt ttgtcaaaaa
     3421 tcaagttgtc tttcttttta aatggggtta aaccaatgat tggtattatc ctcttcacga
     3481 ctttatttca gatgtttttc tcacaaggag gaaaagtaat ttttagttgg tggttcatta
     3541 gcataactga tttaggattg agccaagcta ttttaatttt tatgcgtttc gttctaatta
     3601 ttttcttttc aacgctgtta acgttgacga caacaccttt aagcctatct gatgcagttg
     3661 aatcattatt aaaaccttta acaagattta aagttccagc tcacgagatc ggactaatgc
     3721 tttctttaag tttacgtttt gtgcctactt taatggatga taccacgcgt attatgaatg
     3781 cacaaagggc ccgtggagtt gattttggag agggaaattt aattcaaaaa gttaaatcta
     3841 ttattcctat tttaatccct cttttcgctt caagctttaa aagagccgac gcattagcta
     3901 ttgctatgga agctagaggt tatcagggag gagaagggcg aactaaatat cgtcaattag
     3961 actggcagct aaaagatagt ttagctattg gaatagtttc tttattgggt ttgctacttt
     4021 ttttcttgaa aacctctttg taatattttt tagcacaaaa cttttcttta ttagtaagaa
     4081 gtgaattgta cctcaaaagt tagatacaac atctaatgat tgaggtactt ttcttcattt
     4141 gaggaattga aaaccgctat ctcaactata ttaatcctcc caataataac aaatcgaacg
     4201 aaa
//
LOCUS       DATXQK010000085         4112 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.085, whole genome shotgun sequence.
ACCESSION   DATXQK010000085 DATXQK010000000
VERSION     DATXQK010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4112
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.085"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            38..133
                     /locus_tag="VC394_001679"
     CDS             38..133
                     /locus_tag="VC394_001679"
                     /inference="COORDINATES: protein motif:HMM:NF038244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system DWW-type pheromone"
                     /protein_id="HEP5995125.1"
                     /translation="MLKKYKYYFIFAALLSFKVVQELSAVDWWRL"
     gene            180..1178
                     /gene="ruvB"
                     /locus_tag="VC394_001680"
     CDS             180..1178
                     /gene="ruvB"
                     /locus_tag="VC394_001680"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023079556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEP5995126.1"
                     /translation="MARILDNDVMGNEEFSDRTLRPQYLHEYIGQDKVKEQFAIFIEA
                     AKRRDESLDHVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPAVEKAGDLVAILNELE
                     PGDILFIDEIHRMPMSVEEVLYSAMEDFYIDIMIGAGDTSRSIHLDLPPFTLIGATTR
                     AGMLSNPLRARFGITGHMEYYQEKDLTEIVERTATIFEIKIDHEAARKLACRSRGTPR
                     IANRLLKRVRDYAQIIGDGIITAQITDRALTMLDVDREGLDYIDQKILRTMIEMYQGG
                     PVGLGTLSVNIAEERNTVEEMYEPYLIQKGFLMRTRTGRVATQKAYRHLGYPYQNT"
     gene            1316..1753
                     /locus_tag="VC394_001681"
     CDS             1316..1753
                     /locus_tag="VC394_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEP5995127.1"
                     /translation="MKKVCFVCLGNICRSPMAEFVMKSIVSSDVMMIESRATSDWEHG
                     NPIHSGTQSILKTYQINYDITKCSKQITITDFNTFDYIIGMDSDNVKNLKEMSQHQWD
                     SKIYLFREGGVPDPWYTNDFEETYQLVRKGCQDWLSRLMSKEY"
     gene            1776..2177
                     /locus_tag="VC394_001682"
     CDS             1776..2177
                     /locus_tag="VC394_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5995128.1"
                     /translation="MIDMQDIVKKWSITRAKLEIVSVIVILVCAISVFSVRISNKTSL
                     TYDKGRMHYTGYVINHKMNGEGKLVYPNGDIYEGTFKNGLFEGKGTFTAKTGWLYNGE
                     FHKGQANGKGVLKAKNNKVYKGIFKQGIFQK"
     gene            2174..3949
                     /locus_tag="VC394_001683"
     CDS             2174..3949
                     /locus_tag="VC394_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEP5995129.1"
                     /translation="MRIKWFSFVRVTGLLLVLLYHFFKNVFPGGFIGVDIFFTFSGYL
                     ITALLIDEYTKKESIDIIGFLKRRFYRIVPPLVLMILLTIPFTFLIKKDFIANIGSQI
                     TAVLGFTTNIYEILTGSSYESQFIPHLFVHTWSLAIEVHFYLLWGVFVWLLARRKETQ
                     KQLRGLLFLISLGIFAISFLSMFIRSFMTSNYSLIYFSSLSHSFPFFLGAMFATITGI
                     NETTVRFQKNVRLWPRQYVLAAMIGAFTLLLVLTVTLDFNHITTYLFGFALASLFASI
                     MIYAARVLHEQTPDVQEPKAITYIADISYGIYLFHWPFYIIFSQLMSHILAVILTVFF
                     SILFATVSYYIVEPLVQGRKPNLLGLEIDCSPYYKWIVGGSLALALLTLGTCMIAPKV
                     GKFEKQLLVSSLQQAQSNMERTHTLAAGDANALSDVGIIGDSVALRSSAAFSKIMPQA
                     QLDAAVSRNFKEAFDLFNNQIKSKSLSKTVVLAVGVNSLDNYSQAVQSFIEALPKGHR
                     LVLVSPYNAKNASQVAEARDYGLKLSKKYKYVTIADWYKVAVEHPDIWYGSDGVHYSE
                     DSQGAELYVSTIQTAVEKSAKKPAK"
BASE COUNT         1266 a          639 c          843 g         1364 t
ORIGIN      
        1 cctaatataa aaaataatta ttaaggagtt tttatccatg ttaaaaaagt ataagtacta
       61 ttttatattc gcagctctac tatcttttaa agtagttcaa gagcttagtg ctgttgactg
      121 gtggcgatta taataaaagt aggccactgc tctcttttac tttatgttat aatagtccta
      181 tggctagaat tttagacaat gatgtaatgg gaaatgagga gttttcggat cggactcttc
      241 gccctcaata tctacacgaa tatattggac aagataaagt taaggaacaa ttcgctattt
      301 ttatagaagc agctaaaaga cgtgacgaat ctttagacca tgttttatta tttggacctc
      361 caggactggg aaaaacaaca atggcatttg tgattgccaa tgaattggga gttaatttaa
      421 agcaaacttc aggtcctgcc gttgagaaag ctggagattt ggtagctatt ttaaatgaat
      481 tagaaccagg ggatattttg tttattgatg aaattcaccg catgccaatg tctgttgagg
      541 aagtactata tagtgctatg gaagacttct atattgatat tatgattggc gctggagata
      601 ctagtcgtag tattcatttg gatctgcctc catttacatt gattggtgct actacacgtg
      661 caggcatgtt atctaatccc ctcagagctc gttttggtat cactggtcac atggaatact
      721 atcaagaaaa ggatttgaca gagattgtgg agcgtacagc tactattttt gaaataaaaa
      781 tagaccatga agctgcacgg aaattagctt gtcgcagtag aggaacgccc cgcattgcta
      841 atcgattact aaaacgagtg cgtgactatg ctcaaattat tggtgatggc atcataacgg
      901 ctcaaataac tgatagagct ttgactatgt tagatgttga ccgcgaaggt ctcgattaca
      961 ttgatcagaa aattttacga acgatgattg agatgtatca gggaggacca gttggtttgg
     1021 ggacattatc tgtgaatatt gccgaggagc gtaatacagt tgaagagatg tatgaaccct
     1081 acttaattca aaaaggcttt ctcatgcgaa cacgaacagg tcgtgtagcg actcaaaagg
     1141 cttatcgtca tcttggctat ccctatcaaa acacttaatg atttttcaaa tctaagaaaa
     1201 ttaatctgaa cctgcttttt gtgttcggat tttttgataa gttgacagaa aattattaga
     1261 ttgatttctc cgatatcagt tagaaatttg atataataat tcaaaggagt atttaatgaa
     1321 aaaagtatgt tttgtctgtc tgggtaatat ttgtcgaagc cccatggctg agtttgtaat
     1381 gaagtcaata gtatcaagtg atgttatgat gatagaaagt cgtgctactt ctgattggga
     1441 gcatggaaac cctattcact caggtacaca gtccatatta aaaacttacc aaattaatta
     1501 tgatataact aaatgctcca agcagataac tataactgat tttaacacct ttgactatat
     1561 tattggtatg gatagcgata atgtgaaaaa tctcaaagag atgtcgcaac atcaatggga
     1621 ctctaaaatc tacctgttta gagaaggggg tgttcctgat ccttggtaca ctaacgattt
     1681 tgaagaaacc taccaattag ttagaaaagg atgccaggat tggctttctc gtttaatgag
     1741 taaagagtat tgatattaga agttgagtaa aagaaatgat agatatgcaa gatatagtaa
     1801 aaaaatggag tattacacgg gcgaagttgg aaattgtctc ggtgattgtc atattggttt
     1861 gtgcaatttc tgtttttagc gtaagaattt cgaataagac aagtttgacc tatgataaag
     1921 ggcgtatgca ctatactggt tacgtgatca accataaaat gaatggagaa gggaagcttg
     1981 tctaccccaa cggtgatatc tatgagggta cttttaagaa tggcctattt gaaggaaaag
     2041 gaacttttac tgctaagacg ggctggttat ataacgggga gtttcataaa ggacaagcaa
     2101 atggcaaagg tgtgttaaaa gcaaagaata ataaagtcta taaagggatt tttaaacagg
     2161 ggatatttca aaaatgagaa taaaatggtt ttcgtttgtt agggtaacag gacttttact
     2221 ggttttactc taccactttt ttaaaaatgt atttccagga ggttttattg gtgttgatat
     2281 tttcttcacc ttttcaggat atctaatcac agccctcttg attgatgaat acacaaaaaa
     2341 agagtctatc gatattatcg gttttttaaa aagacgcttt tatcgaattg ttcctccttt
     2401 ggtgttaatg attttattga ccattccttt tacgttttta attaagaagg atttcattgc
     2461 taatatagga agtcagatta cagctgtttt aggatttacc actaatattt atgaaatatt
     2521 gacgggaagt agttatgaaa gccaatttat tccacatctc tttgttcata cgtggagctt
     2581 agctattgaa gttcattttt acttgttgtg gggtgtattt gtttggttat tagctagacg
     2641 caaggagact cagaaacaac tgagaggact actgtttttg atttctttgg gaatatttgc
     2701 tattagtttc ttgagcatgt ttataagatc ttttatgacc tcaaattatt ccctgattta
     2761 tttttcgagt ctttctcaca gttttccctt ctttttaggg gcgatgtttg ccacaataac
     2821 aggtattaac gagactactg ttcgatttca aaaaaatgta cgtttatggc cgcgccagta
     2881 tgtccttgct gctatgattg gagcatttac acttttgctt gtgctaacgg tcacattgga
     2941 ttttaaccat atcacgacct atctatttgg ctttgctttg gctagcttat tcgcatcaat
     3001 tatgatttat gcagcgcgtg ttcttcatga gcaaacacca gatgtacaag agcctaaagc
     3061 tatcacttat atagctgata ttagctatgg gatttacctc ttccattggc cattttacat
     3121 tatttttagc caattaatgt ctcatatttt ggcagttatt ctaacagttt tcttttcaat
     3181 cttgtttgcc actgtgtctt actatattgt ggaacctttg gtccaaggga gaaaaccaaa
     3241 cctgcttggt ttagaaattg actgttctcc ttactataag tggattgtag gagggtctct
     3301 cgccttagca ttattaactt taggaacttg tatgatagcg cctaaagttg gtaagtttga
     3361 gaaacaattg ttggtcagtt ccctacaaca ggctcaaagt aatatggagc ggacacatac
     3421 actagctgca ggagacgcta atgctttgag tgacgtaggt attattggtg attctgtagc
     3481 attacgctct agtgcagcat tctcaaaaat tatgccacaa gcgcaactgg atgcagcggt
     3541 cagtcggaat tttaaagaag ctttcgattt atttaataac caaattaagt ctaagagtct
     3601 atctaagact gttgtcttag cagtaggtgt taactcgctt gacaactatt ctcaagcagt
     3661 tcagtctttt attgaggcat tgccaaaagg acatcgttta gttttggttt ctccatataa
     3721 tgccaaaaat gccagtcagg ttgcagaagc tagagactat ggtcttaaat tgtccaaaaa
     3781 atacaagtat gtcactattg cagactggta taaagttgct gttgaacatc cagatatttg
     3841 gtatggcagt gacggtgttc attatagtga agatagtcaa ggagcagaac tatatgtctc
     3901 cacaatccag actgctgttg agaagtcagc taaaaaacca gctaaataaa gttaagaaat
     3961 gtctaagaga taagtcagat ccattgtgct ctggcttttt cttgtgaaca aatgatgatg
     4021 gtaaaatagt agtactttga ggtttttaaa agaaagcgat ttcagaaata aaacggcttt
     4081 ttttagatat atttctgaaa aagagttaat ga
//
LOCUS       DATXQK010000086         4001 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.086, whole genome shotgun sequence.
ACCESSION   DATXQK010000086 DATXQK010000000
VERSION     DATXQK010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.086"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     misc_feature    4..139
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            168..668
                     /locus_tag="VC394_001684"
     CDS             168..668
                     /locus_tag="VC394_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012658294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEP5995130.1"
                     /translation="MSEAIIAKKAEQVELIAEKMKAAASIVVVDSRGLTVDQDTVLRR
                     SLRESGVEFKVIKNSILTRAAEKAGLDELKDVFVGPSAVAFSNEDVIAPAKVINDFTK
                     TADALEIKGGAIEGAVSSKEEIQALATLPNREGMLSMLLSVLQAPVRNVAYAVKAVAE
                     NKEGAA"
     gene            733..1098
                     /gene="rplL"
                     /locus_tag="VC394_001685"
     CDS             733..1098
                     /gene="rplL"
                     /locus_tag="VC394_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEP5995131.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAAAAA
                     GGAEEAAKDSFDVELTSAGDKKVGVIKAVREITGLGLKEAKGLVDGAPANVKEGVAAA
                     EAEEIKAKLEEAGATITLK"
     gene            1357..1956
                     /locus_tag="VC394_001686"
                     /pseudo
     CDS             1357..1956
                     /locus_tag="VC394_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein"
     gene            2131..2456
                     /locus_tag="VC394_001687"
                     /pseudo
     CDS             2131..2456
                     /locus_tag="VC394_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670032.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
     gene            2447..2721
                     /locus_tag="VC394_001688"
                     /pseudo
     CDS             2447..2721
                     /locus_tag="VC394_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001883480.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2767..2979
                     /locus_tag="VC394_001689"
     CDS             2767..2979
                     /locus_tag="VC394_001689"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5995132.1"
                     /translation="MMTDRVMKKPIRGRQLSKRDLYDEEFFRTHFAKIEIESRLEFLL
                     NRANSLEELLLKAKEVNLTIDLKQKM"
     gene            3244..3941
                     /locus_tag="VC394_001690"
                     /pseudo
     CDS             3244..3941
                     /locus_tag="VC394_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic (srt) production protein"
BASE COUNT         1374 a          627 c          855 g         1145 t
ORIGIN      
        1 taggttttga taaaaaactg acctaagaca gcaggggagc atgctcataa tattcctgcc
       61 gaggcacaaa acgtgattaa agaaaataac gtatttgtac tttcgtgtct aggtttaggt
      121 gcgatttttt tgtacctagc ccaaaaataa aaacggaggt aaaactaatg agtgaagcaa
      181 ttattgctaa aaaagctgaa caagtcgaac ttattgctga gaaaatgaaa gcagcagcaa
      241 gtatcgttgt tgttgattca cgtggtctta cagttgatca agatactgtt cttcgtcgtt
      301 cacttcgtga aagtggcgtt gagttcaaag ttatcaaaaa ctcaattttg actcgtgcag
      361 ctgaaaaagc aggtcttgat gagttgaaag atgtattcgt aggaccatct gcagtagcat
      421 tttctaacga agatgttatc gcaccagcta aagttatcaa cgactttact aaaactgctg
      481 acgcacttga aatcaaaggt ggtgcaatcg aaggcgctgt ttcttcaaaa gaagaaatcc
      541 aagcacttgc aacattgcca aaccgcgaag gaatgctttc tatgcttctt tctgtacttc
      601 aagctccagt ccgcaacgtt gcatacgctg tcaaagctgt tgcagaaaac aaagaaggcg
      661 ctgcttaaga cgtctcagag tagcttaagg ctacgcttaa cattttaaat taattatttg
      721 gaggaaatca caatggcatt gaacattgaa aacattattg ctgaaattaa agaagcttca
      781 atccttgagc ttaacgatct tgtaaaagct atcgaagaag aatttggtgt aactgcagct
      841 gctcctgtag ctgctgcagc tgctggtggt gctgaagaag ctgctaaaga ttcattcgac
      901 gttgaattga catctgctgg cgacaaaaaa gttggcgtta tcaaagctgt tcgtgaaatc
      961 acaggtcttg gtcttaaaga agctaaaggt cttgttgatg gagcacctgc taacgttaaa
     1021 gaaggcgttg ctgctgcaga agctgaagaa atcaaagcta aacttgaaga agctggagca
     1081 acaatcactc ttaaataaga agcagatagc aatttgagag cgaaatcccg atttatcagt
     1141 cttttagagc ttatagcgat ttgaagaaac tgataaaatg atttggtttt ttgccaaaaa
     1201 tcctgccaaa aaaatctttg gcaggatttt tattttctat aaaaattaag ctgagtagag
     1261 tggttattct atctatatgt gtctagtaat aattttttta ttttcaggag gaaaatcatg
     1321 caaatgaatg aacagtcatc acgacaaatg gtgattgcca tctatctcat ttattccaac
     1381 tcaaaatgat agtactcctt ggctgttcca agtcgaaatt gttagtgatt aagaaaacct
     1441 acgagactat ggtttgatcg atgaaggcca gcagtctccc agtgaaaaag ggcgtttggc
     1501 taataagatt tatttaggaa agttggaaca tgatttcacc ccagtcttaa gttcagacgg
     1561 ggccagtctc aaaaacagac cagccccgtg ttaaattcag cccctagtga gactgaaaag
     1621 agtgaaactg aaggtagtga aactaaagag agtaatttag tcattgagga cgaggaggaa
     1681 agaaaggagt gcactagtgt gaaaaagact gagggtcact ttactcgtca agttgatcat
     1741 gtcaccaaat atgacaaaga ttatatttgg agtttggttc attcccaatt acgtgaaggt
     1801 ggtttatcac aagctgctag tgacttagtc atgagctatt ttgaagaacg ctatgcttat
     1861 gcgctggaac acatacggtt cgcaagaaca gctgaagcta ttgcggaata tgtctttaat
     1921 ggtgtcttat ccgaatggac caagcaacta aggtgacaag aagcgaagtg agtaagatgt
     1981 tttggtggct attattaggt gtttggggac taaggacaat ttggttgatt attgaagtta
     2041 tcactgcacc agaaatggaa gattatagtt gagtaaggac gttacataag cagtaaggtc
     2101 cttttatttt gtcagaaagg aatgagagat atgcagtttt tagatttgtt tgcaggaatt
     2161 ggaggctttc gattgggttt aattaatcag tgccatgagt gtattggctt ttgtgagatt
     2221 gataagtttg cgaggcaatc ttacaaagcc atctatgaga ccgaaggaga aatagaattt
     2281 catgacatta gacaagtcac agaccaagac tttagacaac ttagagggca agtggatatc
     2341 atctgcggtg gtttttccct tgtcaagcat tttcactcgc aggcagacga ctgggatttg
     2401 agggcactag gagaactctc ttctttgaga ttactcgagc gaccaaacag ataaaaatca
     2461 tacccataat cacatccaat caacgccatt gaccgtaatt cagataaaaa attgatatgg
     2521 aattatgcct tggaacgaaa cctccgtatg attttagacc gtatttcaaa aatgactgga
     2581 gcaaaaatta tcgagaagcg ttattcctac cgtgactatc aaaaatatag gcagtctagt
     2641 cataaatttg aattgaaaca acgtctttat tttttgatgc agcagtcaaa gttctttgat
     2701 gattttttag aaaaagcaga gtaattacat gttcatattg attttagtca gaagcatagc
     2761 agattcatga tgacggatcg agtaatgaaa aaaccaattc gaggccgtca actcagtaaa
     2821 cgagatttat atgatgaaga attttttcgt acacattttg ccaagataga aattgaaagt
     2881 cgattagaat ttttgttgaa ccgtgccaat tcattagagg agctactatt aaaagcaaaa
     2941 gaagtgaatc taaccattga cttaaaacaa aaaatgtaac ttttatccta gaagaagata
     3001 ctcaaaagat aagtttaggt cataaaaaaa taagcgataa gaaattatat gatgtcaatt
     3061 ttttcaagat tactttaaaa ataaggaagt cggtgtttca gatcgattag aaagtctaca
     3121 gaaacagtac atgcttttca agaagaacga gataaagagg aggtatccac tgaagagagt
     3181 aaggaagtct tcaagaaata caaggggcaa cgagatgcca tccatgagtt tgaagttgaa
     3241 cttgtggaac atcaaattga gaggttaatt gctgaggaca tttatattaa ggtgtctttt
     3301 ggtgtaaagc agagtggtct tattttcatt cctaactatc aattggatat taggaaagaa
     3361 gaaaatcata aaaaatataa agtctatatt cgtgagaccg cacaattctt catttacaac
     3421 aaggaggcgt ctaaacttaa tcgctatatg cgagggcgtg agctcattta ccagttaacc
     3481 aatgatagca agagtattcc aaaaagaaga cgaccaaaac cattgataca cttaagaaaa
     3541 agattgaaga aataagtctc ctcattgagt tagatacgga gaataagcct tatcaagata
     3601 ttaaagatga cattgttaaa gatatggctc aattagattt gaccattaca gagttgcagg
     3661 accatattgc tcatctcaat aaggttgcgg aggtcttgct taatatgaac aacaacgata
     3721 tagagaaccg ccgtttggcc agatatgact atgccaagat gaacttgact gcagctataa
     3781 aaatagaaga agttgagaaa gagattgaaa cttctcaaaa tgaacttaat atatccatag
     3841 atgagtatga atatctagta agaaggttgg aaaagtttgg agagatcttg agttatagca
     3901 aaattatcga tacttctcga aatgaaatac aatgggagta attatgatat aatttgggtg
     3961 gtagcaattt ttatatcaga tattaaaatc tagttgatgt t
//
LOCUS       DATXQK010000087         3986 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.087, whole genome shotgun sequence.
ACCESSION   DATXQK010000087 DATXQK010000000
VERSION     DATXQK010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3986
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.087"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            325..588
                     /locus_tag="VC394_001691"
     CDS             325..588
                     /locus_tag="VC394_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEP5995133.1"
                     /translation="MASKDFHIVAETGIHARPATLLVQTASKFASDITLDYKGKAVNL
                     KSIMGVMSLGVGQGADVTISAEGADAEDAIAAIEETMTKEGLA"
     gene            593..2326
                     /gene="ptsP"
                     /locus_tag="VC394_001692"
     CDS             593..2326
                     /gene="ptsP"
                     /locus_tag="VC394_001692"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEP5995134.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETVTVADTNAEEARL
                     DVALQAAQDELSVIRENAVESLGEEAAAVFDAHLMVLADPEMISQVKETIRAKQTNAE
                     TGLKEVTDMFITIFEGMEDNPYMQERAADIRDVAKRVLAHLLGVKLPNPATINEESIV
                     IAHDLTPSDTAQLNKQFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNDITKRVKDGD
                     VIAVNGITGEVIIDPSEDQVLAFKEAGAAYAKQKAEWSLLKDAHTETADGKHFELAAN
                     IGTPKDVEGVNDNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDLPKEMNPFLGFRALRISISETGDAMFRTQMRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADDIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDSAKMEEYANRALTECSTAEEV
                     LELSKEYVSED"
     gene            2511..3938
                     /locus_tag="VC394_001693"
     CDS             2511..3938
                     /locus_tag="VC394_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012515401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEP5995135.1"
                     /translation="MAKQYKNLVNGEWKLSENEITIYAPATGEELGSVPAMTQAEVDA
                     VYASAKKALSDWRALSYVERAAYLHKAADILVRDAEKIGAILSKEVAKGHKAAVSEVI
                     RTAEIINYAAEEGLRMEGEVLEGGSFEAASKKKIAIVRREPVGLVLAISPFNYPVNLA
                     GSKIAPALISGNVVALKPPTQGSISGLLLAEAFAEAGIPAGVFNTITGRGSVIGDYIV
                     EHEAVNFINFTGSTPIGEGIGKLAGMRPIMLELGGKDSAIVLEDADLVLAAKNIVAGA
                     FGYSGQRCTAVKRVLVMDKVADQLAAEIKTLVEKLSVGMPEDDADITPLIDTSAADFV
                     EGLIKDATDKGATALTAFNREGNLISPVLFDHVTTDMRLAWEEPFGPVLPIIRVTTVE
                     EAIKISNESEYGLQASIFTTNFPKAFGIAEQLEVGTVHLNNKTQRGTDNFPFLGAKKS
                     GAGVQGVKYSIEAMTTVKSVVFDIQ"
BASE COUNT         1250 a          729 c          870 g         1137 t
ORIGIN      
        1 aaagctaaaa agcaagctat aaacttatct aaaacacaac atattgtgtt gaaataaaaa
       61 acagaaacct aaatattgtg tcaatctgga ttcttatggt tagagctcta gcgttttggt
      121 gatagaagct aagcagtttt aataaagcat taaagtctga aaaataaatt gtgaaaaacg
      181 tgaaaataaa agaaaataat ttcacatttt ttgtcgttta aatatttaat cttgaaaata
      241 aaaaagcatt gtgctataat cggttgttgt aagggttatc atgtgatggc tcaaaaatac
      301 acaacttaaa aggagaacca tactatggct tcaaaagact ttcacattgt tgcagaaaca
      361 ggtattcatg cgcgtccagc gactttgctt gttcaaacag ctagcaaatt tgcttcagac
      421 atcactcttg actacaaagg taaagcagta aatctaaaat caatcatggg tgttatgagt
      481 cttggtgttg gtcaaggtgc agatgttact atctcagctg aaggtgcaga tgctgaagat
      541 gccattgcag caattgaaga aacaatgact aaagagggat tggcataaga ctatgacaga
      601 aatgcttaaa ggaattgcag cctcagacgg cgttgctgtt gctaaagcat atctactagt
      661 tcagccggat ttgtcatttg agactgttac agtcgcagat acaaatgcag aagaagctcg
      721 ccttgatgtt gcactccaag ctgcacaaga cgagctttct gttatccgtg aaaatgcagt
      781 agaaagctta ggtgaagaag cagcagccgt ttttgatgcc catttgatgg ttcttgctga
      841 tccagaaatg atcagccagg ttaaagaaac gattcgcgca aaacaaacga atgcagaaac
      901 aggtcttaaa gaagtgactg acatgttcat caccatcttt gaaggcatgg aagataaccc
      961 atacatgcaa gaacgtgcag cggacatccg cgacgttgca aaacgtgtgt tggctcacct
     1021 tttaggtgta aaacttccaa atccagctac aatcaatgaa gaatcaatcg ttatcgcaca
     1081 cgatttgaca ccttcagata ctgctcaact taacaaacaa tttgtaaaag catttgttac
     1141 aaatatcggt ggtcgtacaa gtcactcagc tatcatggca cgtacacttg agatcgctgc
     1201 ggtacttgga acaaatgata ttacaaaacg tgttaaagat ggtgatgtga ttgccgttaa
     1261 tggtatcact ggtgaagtga ttatcgatcc aagcgaagat caagtacttg cttttaaaga
     1321 agctggtgcg gcttatgcca aacaaaaagc agagtggtct ctccttaaag atgcgcacac
     1381 tgaaacagct gatggcaaac actttgaatt ggctgctaat atcggtacgc ctaaagacgt
     1441 tgaaggtgtt aatgacaatg gcgctgaagc tgttggcctt taccgtactg agttcttgta
     1501 catggattct caagacttcc caactgaaga cgaacaatac gaagcttaca aagcagtgct
     1561 tgaaggcatg aatggcaaac ctgttgtggt tcgtacaatg gatattggtg gagataagga
     1621 acttccttac tttgaccttc caaaagaaat gaacccattc ctcggtttcc gtgctcttcg
     1681 tatctccatc tctgaaactg gggatgccat gttccgcaca caaatgcgtg cgcttcttcg
     1741 cgcctctgtt cacggacaac ttcgtatcat gttcccaatg gtagcacttc ttaaagaatt
     1801 ccgtgctgca aaagcagtct ttgacgaaga aaaagcaaac ttgcttgcag aaggcgttgc
     1861 ggttgctgat gacatccaag ttggtatcat gattgagatt cctgcagctg ctatgcttgc
     1921 agaccaattt gctaaggaag ttgatttctt ctcaattgga acaaacgacc ttatccaata
     1981 cactatggca gcagaccgta tgaacgaaca agtatcatac ctttaccaac catacaaccc
     2041 atcaatatta cgtttgatca acaatgtgat caaagcagcg cacgctgaag gtaaatgggc
     2101 aggtatgtgt ggtgagatgg caggtgacca acaagctgtt ccacttcttg ttggaatggg
     2161 cttggatgag ttttctatgt cagcaacttc agtacttcgt acgcgtagtt taatgaagaa
     2221 acttgactct gctaagatgg aagaatatgc aaatcgtgcg cttacagaat gttcaacagc
     2281 agaagaagtt cttgaacttt ctaaagaata cgtttctgaa gattaacggg ttaaagagtt
     2341 ggtttaaacc agctcttttt tgctagctaa aaatacaatt actttttggt gtgaaatcat
     2401 ttgagttatc aaataattat cagaattttc agatatttag tgtagccaaa tcttgaatta
     2461 tatcagaaaa gtgatagaat aagattacta tcataaaagg agataaaacg ttggcaaaac
     2521 aatataaaaa tttagtgaac ggtgaatgga aactatcaga aaacgagatt accatttacg
     2581 caccagcaac aggtgaagag ttaggatcag ttccagcgat gacgcaggca gaggtagatg
     2641 ctgtttacgc ttcagctaaa aaggctctat cagattggcg cgccttgtct tatgtggaac
     2701 gtgcagctta ccttcataaa gcggctgata ttttagtacg tgatgctgaa aagatcggcg
     2761 cgattctttc aaaagaagta gccaaaggtc acaaggcagc tgtcagtgaa gttattcgta
     2821 ccgctgaaat cattaattat gcagcagaag aagggcttcg tatggaaggt gaagttcttg
     2881 aaggtggtag cttcgaagcc gcaagtaaga agaagattgc tattgttcgt cgtgaaccag
     2941 ttggtttagt tcttgccatc tcacctttta attatcccgt taacttggca ggttctaaaa
     3001 ttgctccagc ccttatttca ggaaatgttg ttgctcttaa accaccaaca caaggctcta
     3061 tttctggttt gttactagca gaagcttttg cagaagctgg tattccagca ggtgtcttta
     3121 ataccattac agggcgaggt tctgttattg gggattacat cgttgagcac gaagcggtta
     3181 actttatcaa ctttacaggt tctactccaa ttggggaagg aatcggtaaa ttagcgggta
     3241 tgcgaccaat tatgcttgag cttggcggta aggattctgc tatcgttttg gaagatgcag
     3301 atcttgtttt agcagcgaaa aatattgtag ccggtgcttt tggttactca ggacaacgtt
     3361 gtacagcggt taaacgcgtt cttgtgatgg acaaggtggc ggatcaattg gcggctgaga
     3421 ttaaaacact tgttgaaaaa ctaagtgtcg gaatgcctga agacgatgct gatattacac
     3481 cattaattga tacatcagct gctgattttg ttgaagggtt gattaaagat gcaactgata
     3541 agggagctac tgctttgaca gcctttaatc gtgaaggcaa tcttatttca cccgttctct
     3601 ttgatcatgt gacaactgac atgcgtttgg catgggaaga gccgttcggc ccagtattac
     3661 caattattcg tgtaaccact gtagaagaag ccatcaagat ttctaatgag tctgaatatg
     3721 gtttgcaagc ttctattttt acaactaatt tcccaaaagc ttttggcatt gctgagcaat
     3781 tagaagttgg aactgttcac cttaacaata aaacacaacg tggaacagat aatttcccat
     3841 tcttaggcgc taaaaaatca ggtgcagggg tacaaggagt taaatattct atcgaagcta
     3901 tgacaactgt taaatctgtt gtatttgata tccagtaata aaaaaaataa acaaaaagtt
     3961 aggttaactt aactttttgt ttattt
//
LOCUS       DATXQK010000088         3939 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.088, whole genome shotgun sequence.
ACCESSION   DATXQK010000088 DATXQK010000000
VERSION     DATXQK010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.088"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..615
                     /locus_tag="VC394_001694"
     CDS             <1..615
                     /locus_tag="VC394_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEP5995136.1"
                     /translation="VQRLMKVMGLAARIRRKRKYSSYKGEVGKKADNLIKRHFEGSKP
                     YEKCYTDVTELALPEGKLYLLPVLDGYNCEIIDFTLSRSPNLKQVQTMLEKTFPADSY
                     SGTILHSDQGWQYQHQSYHDFLESKGILPSMSRKGNSPDNGMMDSFFGILKSEMFYGL
                     EKSYKTLDKLEEAITDYIFYYNNKRIKAKLKGLSPVQYRTKSFQ"
     gene            complement(915..1787)
                     /locus_tag="VC394_001695"
     CDS             complement(915..1787)
                     /locus_tag="VC394_001695"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEP5995137.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLASGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDRIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGV
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
     gene            2011..3321
                     /locus_tag="VC394_001696"
     CDS             2011..3321
                     /locus_tag="VC394_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEP5995138.1"
                     /translation="MIHLIIISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPNALYMILYLIAT
                     LLSALGAGFFTTMAICCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSGSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYIRYSKGRTNSSLTIDQPPVLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYRQTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWAIIIMLCGMSLLMSLAVKSGLVTLIGHLITTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPTLFPIIATISTQSPHIDIRLLTTATIIGALSTNISPFSSAGSL
                     IQLSLPHIEERSLAFKKQILLGVPISLSLALLTIWILMLLASLS"
     gene            3399..3692
                     /locus_tag="VC394_001697"
     CDS             3399..3692
                     /locus_tag="VC394_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEP5995139.1"
                     /translation="MPRKTFDKAFKLSAVKLILEEEQSVKMVSSTLEIHPNSLYRWIQ
                     EYEKYGESAFPGHGSALRHAQFETKKLEKEHKLLQEELALLKKFQVFLKPNRK"
     gene            3725..>3939
                     /locus_tag="VC394_001698"
     CDS             3725..>3939
                     /locus_tag="VC394_001698"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEP5995140.1"
                     /translation="MNSHHTCQTLQVSRSGFYAYLKRRLSSKHVENEALKEMIKDIFY
                     EHKERYGSVRITQELCRRGIHVNHKRVG"
BASE COUNT         1174 a          881 c          709 g         1175 t
ORIGIN      
        1 gttcaacgtc tcatgaaagt catgggccta gcagcccgca ttcgtcggaa gcgcaagtat
       61 tcttcttaca aaggagaggt tggtaagaag gctgataatc tgattaaacg tcacttcgaa
      121 ggctctaaac cttacgaaaa atgttacact gatgtgacgg agttggcctt acctgaagga
      181 aaactctact tattacctgt tcttgacggc tataactgtg agattattga tttcactctg
      241 tctcggtctc ctaacttgaa acaggttcaa accatgcttg agaagacttt tccagcggat
      301 tcatacagcg gaactattct tcatagtgac caggggtggc aataccaaca ccagtcctac
      361 catgattttt tagagtctaa aggtattctg ccatccatgt cccgcaaagg gaatagcccg
      421 gataacggca tgatggattc cttctttggc attctcaaat ctgaaatgtt ttatggactc
      481 gaaaagtctt ataaaaccct tgacaagctt gaagaagcta ttacagatta cattttttac
      541 tacaacaaca aacgaatcaa agcaaaacta aaaggactta gtcctgtcca atacagaact
      601 aaatcctttc aataattatt tgtccaactt tttggggtca gtacactcct agttaacgac
      661 tttgattgat tttattatga tgaatcgtga tttctgaaaa caaaattttc ttcatggatt
      721 tggcgtggaa cttttacaat aaaaacacta acctctccct atcgtttttt caggtgaatg
      781 cttggaattg gtataataag cggacacgaa tcaggtgaaa acagagaaag tatttgaaga
      841 ggcgtttaaa ctatctgaca taaaactcgt tctagaggaa agacatcctt taaaaagtta
      901 gtttattttg caacttagac atcaaggtag gttaaaccct tcatggaaaa atcgagactc
      961 ttagcactat gggttaaact accactggag acgtaatcaa tcgctaaacc acgaaaacgg
     1021 ctaatagtgg tcatatcaat atttccagaa cattcaatcc gagaacgtcc tgcaattaga
     1081 gtaatggcct gttcaatctg ttccaatgac atattatcca acatgataat atcaacaccc
     1141 gccgccgcag cttcttcggc agcagcaaga ctttccactt ccacctcgac cattttcaca
     1201 aaaggggcat aggcacgcgc ttgagcaatc gccttttgaa cactacctac tgccgcaatg
     1261 tgattgtctt ttagcatgat agcatctgat aaattaaagc gatgattata gccaccgcca
     1321 actctcacgg catatttctc aaaaagacgt aaattaggag tggtttttcg agtatcaaat
     1381 accttaatgc gatcatcgcc taaggcttct acataagcag ctgtcatcga agcaatccct
     1441 gataaatgtt gtaaaaaatt caaggcaacg cgttcacatg ttaagaggct tctcaccgag
     1501 cctatgattt ctaaaaccaa atcgccacta gccaaacgat ctccatcctt aaattgatga
     1561 ggattctgga aggtcacctc ggcatcaaat agggtaaaaa ccctttgaaa aacggttagc
     1621 cccgctaaaa caccagcttc cttggcaaaa agcgacacct tggcttggcc atgatgatca
     1681 aaaatggcat tggtactgta atcttcggaa tgaacatctt ctcgcaaggc tgctttcaat
     1741 gtatcatcta tttgaaaagg ggttaaatca gttgaaatga ttgacatcac taagtcctct
     1801 atttttaata tcacttgtaa gcactagagg caattgctat taacttccgt aggaaatcac
     1861 aaacctgaat acctcgtttt aaattccaac aagtgtcaag acaattgtaa tttttttgtg
     1921 tataatagtc aagtaccaat aggaaacagg tggtcttttg agcgccaaaa ttgccaacct
     1981 atttacatat tctaactaaa ggacaattca atgattcatt taataataat tagcgccatt
     2041 gctctagcca ttggaattgg ttaccgcacc aaaatcaata ttggcctact ggctattgct
     2101 ttttcttacc tcatcgcaac cactctcatg ggattaagcc ccaaagaact tcttcatttt
     2161 tggccaacct cactcttttt taccattttt agcgtctctc tcttttataa cgttgcaaca
     2221 actaacggca ctcttgatgt tttggctcaa cacattctct accgcacacg cacccaccct
     2281 aacgccctct acatgatttt atacctgata gcaacccttt tgtctgcttt aggtgctgga
     2341 tttttcacta ctatggccat ttgctgtcct ctagcgatta ccctctgtca aaaagcggac
     2401 aaacaccctt tgattggagc tcaagccgtc aattggggag cttcaggagg ggctaatttg
     2461 ataaccagtg gctcaggcat tgtctttcaa ggcctgttta agcaaatggg atgggaagag
     2521 caagcttttt cacttggcaa tcatatcttt atcgtcagca ttatctaccc actcatcgtc
     2581 ttgcttttat tatcttgtta catccgctac agtaagggaa gaacaaactc atccttgact
     2641 attgaccaac caccagtcct cagcaaggtc caaagacaaa ccaccttgct catgatcagc
     2701 agcatggttt tggtttggct ttttcccttg ttgcatctca ttttcccaaa catcgcatgg
     2761 atagctactt atcgacaaac atttgatatt ggctttgtct caatactcat ggtctgcctc
     2821 gccttaaggc tcaaattagg gaagcaagaa gccattttgg ctaaggttcc ctgggctata
     2881 attatcatgc tctgcggcat gagcttacta atgtctctag cggtcaagtc aggtttagtt
     2941 acactgatag ggcacctcat aacaacaacc attcctcatt tctggttacc tctgttcttt
     3001 tgtgtgatag ccggagtgat gtcactcttt agttccaccc tatccgttgt agccccaact
     3061 ctctttccca tcattgctac catcagtact caaagtcctc acattgatat tcgcttactc
     3121 acaacagcca ctattattgg cgctctatct actaatatct ctcctttttc atcagctggt
     3181 tccctcattc aactctccct tccccatata gaggaacgca gtcttgcttt caaaaaacaa
     3241 attcttctgg gtgtgcccat cagcttaagt ttggcactct tgaccatctg gattctcatg
     3301 ctgctagctt ccttaagtta gatttattgt ctaacatttg gttggacttg gtatactaaa
     3361 gtagacacaa aattaagtgg aaacgaggaa tagtcattat gccaagaaaa acctttgata
     3421 aagccttcaa actctctgct gtaaaactca tccttgagga agagcagtct gtaaaaatgg
     3481 ttagttcaac tttagaaatt catcccaata gtctttatcg atggattcaa gaatatgaaa
     3541 aatatggaga aagtgcgttc ccaggacatg ggagcgcact tcgtcatgct caatttgaga
     3601 caaaaaaact tgaaaaagag cacaaactgc tacaggagga attagctctt ctaaaaaagt
     3661 tccaggtctt tttgaagcca aaccggaaat aaaatttcag tttctgaagg aaaatagtgt
     3721 tagcttgaat agtcatcaca cctgtcaaac tcttcaagtc tcccgttcag gtttctatgc
     3781 ttatttaaaa cgtcgtctat cttcaaagca tgttgagaat gaagctttga aggaaatgat
     3841 taaagacatt ttctatgaac ataaagagcg ttacggaagt gtacgaatta cccaagaact
     3901 ttgtagacgt ggcatccatg tcaatcataa acgtgttgg
//
LOCUS       DATXQK010000089         3922 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.089, whole genome shotgun sequence.
ACCESSION   DATXQK010000089 DATXQK010000000
VERSION     DATXQK010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.089"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..1916
                     /gene="ftsH"
                     /locus_tag="VC394_001699"
     CDS             <1..1916
                     /gene="ftsH"
                     /locus_tag="VC394_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEP5995141.1"
                     /translation="VITGFQFYLKGTSTQSQQISYSKLIKHLKAGDIKSLSYQPSGSI
                     IEVKGKYEKPQKVTVNSGLSFLGGRASTQVTEFSSLVLPSDTILKEMTAAADKNGTEL
                     TVKQESSSGTWITFLMSFLPIVIFAAFMMMMMNQGGGGARGAMSFGKNKAKSQSKGDV
                     KVRFTDVAGAEEEKQELVEVVDFLKNPKKYKSLGARIPAGVLLEGPPGTGKTLLAKAV
                     AGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAKKAERAIIFIDEIDAVGRRRGAG
                     MGGGNDEREQTLNQLLIEMDGFEGNENIIVIAATNRSDVLDPALLRPGRFDRKVLVGR
                     PDVKGREAILRVHAKNKPLANDVNLKVVAQQTPGFVGADLENVLNEAALVAARRNKIK
                     IDASDIDEAEDRVIAGPSKKDRTISQKEREMVAYHEAGHTIVGLVLSNARVVHKVTIV
                     PRGRAGGYMIALPKEDQMLLSKEDLKEQLAGLMGGRVAEEIVFNAQTSGASNDFEQAT
                     QIARAMVTEYGMSEKLGPVQYEGNHAMMPGQISPEKSYSAQTAQMIDDEVRELLNQAR
                     NQAADIINENRDTHKLIAEALLKYETLDAAQIKSIYETGKMPVDLETDDNEAHALSYD
                     EIKNKMTESESAE"
     gene            2243..3634
                     /locus_tag="VC394_001700"
     CDS             2243..3634
                     /locus_tag="VC394_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEP5995142.1"
                     /translation="MTIFRKKKKYSNKTEMQRHFKVIDLVFLGLGSMVGTGIFTVTGI
                     GAAKYAGPALTISIIISAIAIGILALFYAEFASRMPSNGGAYSYVYATLGEFPAWLVG
                     WYIIMEFLTAISSVAVGWGSYLKGLLANYNIVLPNALNGTFNLKNGTYIDILPVLVMF
                     FVTGIVLMNSKLALRFNSFLVILKFSALALFIFVGIFFIDHNNWSHFAPYGVGQITGG
                     KTGIFAGASVMFFAFLGFESISMAVDEVKEPQKTIPKGIILSLIIVTALYIVVTTILT
                     GIVHYTKLNVPDAVAFALRNIRLYWAADYVSIVAILTLITVCISMTYALARTIYSISR
                     DGLLPKSLYTLTKKNKVPQNATLVTGLLAMICAGIFPLSSLAEFVNICTLAYLIILSG
                     AIIKLRRIEGEPKANEFKTPLVPFLPMLAIIICLSFMSQYKAFTWIAFAIATIIGTLI
                     YLAYGYTHSIENK"
BASE COUNT         1277 a          588 c          765 g         1292 t
ORIGIN      
        1 ttgtcatcac aggatttcaa ttttatctta agggaaccag cacacaaagc caacagatta
       61 gttattccaa attaattaaa catcttaagg ctggagatat aaaatcttta agttatcagc
      121 ctagcggtag tattattgaa gttaaaggga aatatgagaa acctcaaaaa gtgacagtta
      181 attcaggact atcattttta ggtggaagag cttcaacaca ggtaacagag ttctcatctt
      241 tagtattacc tagtgatacc atcttgaaag aaatgacagc agcagctgat aaaaatggaa
      301 cagagcttac agttaagcag gaaagttcaa gtggtacttg gattaccttc ttaatgagct
      361 ttttacctat tgttattttt gctgctttca tgatgatgat gatgaatcaa ggtggtggag
      421 gagctcgtgg tgctatgagt ttcggcaaaa ataaggcaaa atctcaatca aaaggcgatg
      481 ttaaagtgag atttacagac gttgctggtg ccgaagaaga aaaacaagaa ttagtagaag
      541 ttgttgattt tttgaaaaat cctaaaaaat ataaatcact aggagcacgg atcccagcgg
      601 gagttcttct agaggggcct ccaggtactg gtaaaacatt actagctaaa gctgtagcag
      661 gtgaagctgg agtgccattt ttcagtattt caggttcaga ttttgtcgaa atgtttgtcg
      721 gtgtaggtgc tagtcgtgtt cgttctttat ttgaggatgc taaaaaagct gagcgtgcaa
      781 tcatctttat cgatgaaatt gacgctgtag gtcgtcgtcg tggtgctggc atgggaggcg
      841 gtaacgatga acgagaacag acccttaacc aattactcat tgaaatggat ggttttgagg
      901 gtaatgagaa cattatcgtt attgctgcaa ctaaccgtag tgatgtttta gatccagctt
      961 tactaagacc aggtcgtttt gatcgtaagg ttttagttgg tcgccctgat gttaaaggac
     1021 gtgaagccat tttacgtgtt catgctaaaa ataaaccact tgcaaatgac gttaatttga
     1081 aagtagtagc ccaacagact ccaggttttg taggtgctga tcttgaaaat gttttaaatg
     1141 aagctgcgtt ggttgctgct cgcagaaata aaattaaaat tgacgctagt gatattgatg
     1201 aagctgaaga tcgtgttatt gctggaccat caaagaaaga tcgcactatt tcgcaaaaag
     1261 agcgagaaat ggttgcttat cacgaagctg gtcatactat tgttggttta gttttatcta
     1321 atgcgcgtgt agttcacaag gtaactattg tacctcgtgg acgagcaggt ggttatatga
     1381 tcgctcttcc aaaagaagat caaatgcttc tttctaaaga agatctgaaa gagcaattag
     1441 caggattgat gggtggtcgt gttgctgaag aaattgtttt taatgctcaa acatcaggtg
     1501 cgtcaaatga ctttgagcaa gctactcaga tagcaagggc catggttacg gaatatggta
     1561 tgagtgaaaa gcttggacca gttcaatatg aaggtaacca tgcgatgatg ccaggacaga
     1621 tctctcctga aaaatcttac tctgcccaaa cagctcaaat gattgatgat gaggttcgtg
     1681 aactcttaaa tcaagctcgt aatcaagcag ctgatatcat caatgagaat cgagatactc
     1741 ataaattgat tgctgaagct cttctcaaat atgaaactct agacgctgca caaattaaat
     1801 caatttatga aactggtaaa atgccagttg atttagaaac tgatgataat gaagctcatg
     1861 cgttatctta tgacgaaatt aaaaataaaa tgacagaatc tgaatctgca gaatgatata
     1921 aaaaagctga acttatagat taagttcagc ttttttatca acacaatatc cttgtaaacg
     1981 ttatgaattg taataggtgc ctaattgcta gaaatgagaa gatgacatac ttttttttct
     2041 ttattactta aagtgagaaa gactttggta tttaaaacta gaaaattgtt aaagccattt
     2101 gaaaaaatct attagtgttt tcttagaata atagaagaac agaagatagc aaaagtttca
     2161 gtcaaccaat aagatgaaat ttacaatcat tactaactat gatacactta gtagaaaatc
     2221 atttactata gaaggtgata acatgactat ttttcgtaaa aaaaagaaat attccaataa
     2281 aacagaaatg caaaggcact tcaaagttat tgatttagtt tttttaggcc taggttcaat
     2341 ggttggaaca ggtatcttta cagttactgg cataggcgct gctaagtatg caggtcctgc
     2401 tttaacaata tcgattatca tttccgcgat tgctattgga attttagcct tattttatgc
     2461 agaatttgcg tctcgtatgc cttctaacgg tggtgcttat agttatgttt atgcaacctt
     2521 gggagagttt ccagcatggc ttgttggctg gtatattatt atggaatttt taacagctat
     2581 atctagtgtt gctgttggct ggggaagtta tttaaaagga ttacttgcca attataatat
     2641 agtattacca aacgctttaa atggcacgtt caatcttaag aatggaactt acatagatat
     2701 tcttcctgtt ttagtgatgt ttttcgtgac agggattgtc ctaatgaatt cgaaattagc
     2761 cctgagattc aatagttttt tagttatttt aaaattttca gcactggctt tatttatctt
     2821 tgtaggcatt ttttttattg atcacaataa ttggtctcat tttgctccat atggtgtagg
     2881 acaaattact ggaggtaaaa caggtatctt tgctggtgct tctgttatgt tctttgcatt
     2941 tttaggattt gaatctattt caatggcagt tgatgaagtg aaagaaccac aaaaaacaat
     3001 tcctaaaggt atcattttat ctttgattat tgtaacagca ctctatatag ttgtcaccac
     3061 tattttaacg ggaattgtac actacactaa attaaatgtt ccagacgcgg tggcttttgc
     3121 tttaagaaat attagacttt attgggcagc tgactatgtg tcgatagttg ctattttgac
     3181 tcttattacg gtatgtattt ctatgaccta tgcattagct agaacaattt acagtattag
     3241 tagagatggt ttattaccta aatcacttta tactctcact aaaaaaaata aagtgcctca
     3301 aaatgctact cttgtcactg gactacttgc tatgatttgt gcgggaatat ttcctttatc
     3361 gagtctagca gagtttgtta atatttgtac tttagcatat ttaattattc tgtcgggtgc
     3421 aattattaaa ctcagaagaa tagaaggaga accaaaagca aatgaattta aaactcctct
     3481 agtaccattc ctaccaatgt tggcaattat catttgtcta tcatttatga gccaatataa
     3541 agcttttact tggattgctt ttgctattgc cacaattatt gggacattaa tctacttagc
     3601 ttatggttat actcattcaa tagaaaataa ataatgtttt gataataaga tttgacatta
     3661 cattatagac tataatgttt agaagtaaat gagtagtaaa atcaatacct acttacttat
     3721 caaagttgta atatttaaaa ttacccaacc cctagagaat tgattagtct ctaggggttg
     3781 attggtacaa tgacatagac tctaaaacga ttttaatact aaatatgata gatataactt
     3841 tcaaaaaaga aaattagttc atttttgtta cacaataaaa tttgttattt agcagtaata
     3901 taattgaaaa acagaaacat tt
//
LOCUS       DATXQK010000090         3824 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.090, whole genome shotgun sequence.
ACCESSION   DATXQK010000090 DATXQK010000000
VERSION     DATXQK010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3824
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.090"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            36..1259
                     /locus_tag="VC394_001701"
     CDS             36..1259
                     /locus_tag="VC394_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEP5995143.1"
                     /translation="MPSMKYILKSLSILLIGFLGGLIAIITFNNLYPHSPSKINSGKA
                     TTSNMVFNNTTNTTKAVKAVQNAVVSVINYQDNPSSSLSNPYTKLFGEGRSKENKDAE
                     LSIFSEGSGVIYRKDGNSAYVVTNNHVIDGAKRIEILMADGSKVVGELVGADTYSDLA
                     VVKISSDKIKTVAEFADSTKLNVGEVAIAIGSPLGTQYANSVTQGIVSSLSRTVTLKN
                     ENGETVSTNAIQTDAAINPGNSGGPLINIEGQVIGINSSKISSTPTGSNGNSGAVEGI
                     GFAIPSTDVIKIIKQLETNGEVIRPALGISMVNLNDLSTNALSQINIPTSVTGGIVVA
                     EVKEGMPASGKLAQYDVITEIDGKTVNSISDLQSSLYGHDINDTIKVTFYRGTTKKKA
                     DIKLTKTTQDLTKTQ"
     gene            1318..2124
                     /locus_tag="VC394_001702"
     CDS             1318..2124
                     /locus_tag="VC394_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEP5995144.1"
                     /translation="MFLILSRNSLMTKELLIDLPIEDIVTNPYQPRIQFNQRELQDLA
                     TSIKSNGLIQPIIVRKSDIFGYELVAGERRLKASKMAGLKKVPAIIKKISTLESMQQA
                     IVENLQRSNLNAIEEAKAYQLLVEKKHMTHDEIAKYMGKSRPYISNTLRLLQLPAPII
                     KAIEEGKISAGHARALLTLSDDKQQLYLTHKIQNEGLSVRQIEQLVTSTPSSKLSKKT
                     KNIFATSLEKQLAKSLGLSVNMKLTANHSGYLQISFSNDDELNRIINKLL"
     gene            2357..3712
                     /gene="dnaA"
                     /locus_tag="VC394_001703"
     CDS             2357..3712
                     /gene="dnaA"
                     /locus_tag="VC394_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012657571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEP5995145.1"
                     /translation="MTENEQIFWNRVLELAQSQLKQATYEFFVHDARLLKVDKHIATI
                     YLDQMKELFWEKNLKDVILTAGFEVYNAQISVDYVFEEDLMIEQNQTKINQKPKQQAL
                     NSLPTVTSDLNSKYSFENFIQGDENRWAVAASIAVANTPGTTYNPLFIWGGPGLGKTH
                     LLNAIGNSVLLENPNARIKYITAENFINEFVIHIRLDTMDELKEKFRNLDLLLIDDIQ
                     SLAKKTLSGTQEEFFNTFNALHNNNKQIVLTSDRTPDHLNDLEDRLVTRFKWGLTVNI
                     TPPDFETRVAILTNKIQEYNFIFPQDTIEYLAGQFDSNVRDLEGALKDISLVANFKQI
                     DTITVDIAAEAIRARKQDGPKMTVIPIEEIQAQVGKFYGVTVKEIKATKRTQNIVLAR
                     QVAMFLAREMTDNSLPKIGKEFGGRDHSTVLHAYNKIKNMISQDESLRIEIETIKNKI
                     K"
BASE COUNT         1361 a          657 c          644 g         1162 t
ORIGIN      
        1 tataagctat ggacaaataa ttcggaggac aaaacatgcc aagtatgaaa tatatcttaa
       61 aatccttaag tattttacta attggatttt taggaggatt aatagcaatt attacattca
      121 ataatctcta cccacattct ccttcaaaaa taaattcagg taaggcaaca actagtaata
      181 tggtttttaa caacacaacc aatactacta aagctgtcaa agctgttcaa aatgcagttg
      241 tatcagttat taattatcaa gataaccctt cttcatccct ttctaaccct tatacaaaac
      301 tctttggaga agggcgttca aaagagaata aggatgctga attatctatt tttagtgaag
      361 gatctggggt catttatcga aaagatggca actccgctta cgttgttact aataaccatg
      421 ttatcgacgg agctaaacga attgaaattc ttatggcaga cggatctaaa gttgttggtg
      481 aattagttgg agctgatact tattcggatt tagctgttgt taagatctct tcagataaga
      541 taaaaacagt agctgaattt gcagactcta caaaactaaa tgttggagaa gttgctattg
      601 ctatcggcag cccactagga acacaatacg ctaattctgt tactcaagga atcgtctcta
      661 gtttaagtcg tactgtaact ttaaaaaacg agaatggtga gactgtctca acaaatgcta
      721 ttcagacaga tgctgctatt aaccctggaa actctggtgg accactaatt aatattgagg
      781 ggcaagtaat cggaattaat tcaagtaaaa tttcttctac cccaactggt agcaacggta
      841 atagtggtgc tgttgaagga attggttttg ctatcccatc tactgacgtt attaaaatta
      901 ttaaacaact agaaactaat ggtgaagtta tcagacctgc tcttggaata tccatggtca
      961 atttaaatga cttatccaca aatgctctta gccaaattaa tattccaact agtgtaactg
     1021 gtggtattgt agtagcagaa gttaaggaag gaatgccggc atctggcaaa cttgctcagt
     1081 acgatgtgat tacagaaatt gatggcaaaa cagttaattc aattagtgat ttacaaagta
     1141 gtctatacgg acacgatatt aatgatacta ttaaagtaac tttttataga ggaactacaa
     1201 agaaaaaagc agatatcaaa ttaacaaaaa ctactcaaga tttgactaaa acgcagtaaa
     1261 caagctacct aaatactatt acattttatc acataaaaca gtccaaagga ctgttttatg
     1321 tttcttattc tatccaggaa ctcccttatg acaaaagaat tattgataga cctaccaatt
     1381 gaagatattg ttacaaatcc ctatcaacca aggatacaat ttaatcaaag agaactccaa
     1441 gacctagcaa cttctattaa atcaaacggc ctcattcaac ctattattgt tcgaaaatct
     1501 gacatttttg gctatgagtt agttgctgga gaacgacgac ttaaagcttc aaaaatggct
     1561 ggtctaaaaa aagttccagc tatcatcaag aagatctcta cactcgagag tatgcaacaa
     1621 gctatagttg aaaatttaca acgttctaac cttaacgcta tcgaagaagc taaagcctat
     1681 cagttattgg ttgaaaaaaa acacatgact cacgatgaga ttgctaaata tatgggaaaa
     1741 tcaagacctt atattagcaa taccttacgt ctgttacaac tcccagcacc tatcattaaa
     1801 gcaattgaag aaggaaaaat tagtgcggga cacgcgcgtg ctcttttaac tttgagtgat
     1861 gataagcaac aactgtacct cactcataaa atacaaaatg aaggcctaag tgttaggcaa
     1921 attgagcaac tggttacttc tactccaagt tcgaagctat ctaaaaaaac taaaaatatt
     1981 tttgccactt ctttagagaa acaattggct aaatcattgg gactctctgt caatatgaag
     2041 ctgacagcaa accatagtgg gtaccttcag atatcttttt ccaatgatga tgaattaaac
     2101 agaattatca acaagctact ttagcttgtt gatattctgt tttttctttt ttagttttcc
     2161 acatgaaaaa tagttgaaaa caatagcggt gtcacattaa aatggctttt ccacaggttg
     2221 tggagaaccc aaattaacag tgttaattta ttttccacag gttgtggaaa aactaactat
     2281 tatccatcgt tctgtggaaa actagaatag tttatggtag aatagtgcta gaattatcca
     2341 caagaaggaa cctagtatga ctgaaaatga acaaattttt tggaacaggg tcttggaatt
     2401 agctcagagt caattaaaac aggcaactta tgaatttttt gttcatgatg cccgtctatt
     2461 aaaggtcgat aagcatattg caactattta cttagatcaa atgaaagaac tcttttggga
     2521 aaaaaatctt aaagatgtta ttcttactgc tggttttgaa gtttataacg ctcaaatttc
     2581 tgttgactat gttttcgaag aagacctaat gattgagcaa aatcagacca aaatcaatca
     2641 aaaacctaag cagcaagcct taaattcttt gcctactgtt acttcagatt taaactcgaa
     2701 atatagtttt gaaaacttta ttcaaggaga tgaaaatcgt tgggctgttg ctgcttcaat
     2761 agcagtagct aatactcctg gaactaccta taatcctttg tttatttggg gtggccctgg
     2821 gcttgggaaa acccatttat taaatgctat tggtaattct gtactattag aaaatccaaa
     2881 tgctcgaatt aaatatatca cagctgaaaa ctttattaat gagtttgtta tccatattcg
     2941 ccttgatacc atggatgaat tgaaagaaaa atttcgtaat ttagatttac tccttattga
     3001 tgatatccaa tctttagcta aaaaaacgct ctctggaaca caagaagagt tctttaatac
     3061 ttttaatgca cttcataata ataacaaaca aattgtccta acaagtgacc gtacaccaga
     3121 tcatctcaat gatttagaag atcgattagt tactcgtttt aaatggggat taacagtcaa
     3181 tatcacacct cctgattttg aaacacgagt ggctattttg acaaataaaa ttcaagaata
     3241 taactttatt tttcctcaag ataccattga gtatttggct ggtcaatttg attctaatgt
     3301 cagagattta gaaggtgcct taaaagatat tagtctggtt gctaatttca aacaaattga
     3361 cacgattact gttgacattg ctgccgaagc tattcgcgcc agaaagcaag atggacctaa
     3421 aatgacagtt attcccatcg aagaaattca agcgcaagtt ggaaaatttt acggtgttac
     3481 cgtcaaagaa attaaagcta ctaaacgaac acaaaatatt gttttagcaa gacaagtagc
     3541 tatgttttta gcacgtgaaa tgacagataa cagtcttcct aaaattggaa aagaatttgg
     3601 tggcagagac cattcaacag tactccatgc ctataataaa atcaaaaaca tgatcagcca
     3661 ggacgaaagc cttaggatcg aaattgaaac cataaaaaac aaaattaaat aacatgtgga
     3721 aaagaatatc ttttatgaaa tagttatcca caagttgtga acaaccattt agtcttggat
     3781 tctctcgttt atttagagtt atccactata tacacaagac ctac
//
LOCUS       DATXQK010000091         3072 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.091, whole genome shotgun sequence.
ACCESSION   DATXQK010000091 DATXQK010000000
VERSION     DATXQK010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.091"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            279..512
                     /locus_tag="VC394_001704"
     CDS             279..512
                     /locus_tag="VC394_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HEP5995146.1"
                     /translation="MIKFAEEIQKEELFHIIGGYSATDCKNHLIGGITSGAIAGGVGA
                     GMATLGVGGVAGAFAGAIAGGLTCVGGMLFNGK"
     gene            527..709
                     /locus_tag="VC394_001705"
     CDS             527..709
                     /locus_tag="VC394_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IIb bacteriocin, lactobin A/cerein 7B
                     family"
                     /protein_id="HEP5995147.1"
                     /translation="MDNFLELQFEELVNISGGKGNIGSAIGGCLGGMLIAAAGGPITG
                     GAAAFVCVASGIAAYQ"
     gene            complement(900..1585)
                     /locus_tag="VC394_001706"
                     /pseudo
     CDS             complement(900..1585)
                     /locus_tag="VC394_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191693.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1904..2131
                     /locus_tag="VC394_001707"
     CDS             1904..2131
                     /locus_tag="VC394_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HEP5995148.1"
                     /translation="MEIKKLETFHQMTIEKLAKVEGGKNNWQANVSGVIAAGSAGAAI
                     GFPVCGVACGYIGAKTAITLWAGVTGATGGF"
     gene            2144..2344
                     /locus_tag="VC394_001708"
     CDS             2144..2344
                     /locus_tag="VC394_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IIb bacteriocin, lactobin A/cerein 7B
                     family"
                     /protein_id="HEP5995149.1"
                     /translation="MTTMKELTINDMASISGGNAPGDAVIGGLGGLASGLKFCKLPHP
                     VLAGGCVVGFTVGGAYLGYTAN"
BASE COUNT          981 a          418 c          639 g         1034 t
ORIGIN      
        1 tattttcgaa gactataata ttaagttcgg agctcgtatt cataaaggat tcggagtatg
       61 ttttgacaca cttgatagct tatttgacta atattcaaaa actctactct tgtgtttaca
      121 caacattatt gacagtctct aatatgttaa aaagtttatt tgagatgttt atacaaccat
      181 tcacgacata gaaaaaaatt tttataataa tatgctatat ttagtttgtc aaagttgcaa
      241 caagatgaat ataacatttt agaaaggttt ttaaagttat gataaaattt gcagaagaaa
      301 tacaaaaaga agagttgttc catattattg gaggttatag tgctacagat tgtaaaaatc
      361 atttaattgg tggtattact tcaggtgcta ttgcaggtgg tgttggagca ggtatggcta
      421 ctttaggtgt tggaggagtt gcaggtgcgt ttgcaggtgc tattgcgggt ggattaactt
      481 gcgttggtgg tatgttattt aatggaaaat aattttgagg tgaaaaatgg ataatttttt
      541 agaattacag tttgaagaat tagtaaatat ttctggtgga aaaggaaata ttggatctgc
      601 tatcggaggg tgcttaggtg ggatgcttat agctgcagca ggaggtccaa taactggagg
      661 tgcggcagca tttgtatgtg tagcatctgg aattgcagct tatcaatgat atgattatag
      721 ttataatact cggtttaatt tgtctaatac tttcccaaat ttttttgaag gataaagagg
      781 taagagaaaa tgcaagtaaa gtaattttaa ttgtgatatt tgtttatgtc ttactaagta
      841 ctattacaaa ttaataatat taaaatgttg tgtaaacaca agagtagagt ttttgaatat
      901 tagtcaaata aactatcaag tgtgtcaaag catgctccga atcctttatg aatacgagct
      961 ccgaacttaa tattatagtc ctcgaaaata cttacaagac atcgttctaa ggcttcttca
     1021 tttgggaaaa ccactctctt cttgttttgt cgcttaattt ctttattgag tgattcaatc
     1081 aagtttgtag agtaaatgct agaccaaatg tgatgtggga attgatagaa agtaaggaga
     1141 ttttccgtcg tttcaagtga tgccatgacc tttttataac gaggcttcca ttcagaaaca
     1201 agtggtgacc atacatgcgc tcaataatgt cactgatttc gcgtgtcgta acgccagtct
     1261 gatagagttt gatcaccatc tcttccaagt ggtcatctcg tctagcataa gatggtaata
     1321 aaactggtga aaactcgccg tttcgatctc ttgggatgat taaattgact aagccatact
     1381 ttgtttcaaa ctgacgagaa taagtcccat tacggctatt acctgtgttg taaccttctt
     1441 tttcataagg ttcataacca agaaaagctg atagttcaac ttgcaagagg tcattcatag
     1501 ctatttctaa agaggagcga aagaattcat caatatcttg tttttgtgct aggaagttaa
     1561 gtaattctgt ggtaaactga gtcatgggaa taaatctctt tctagtgaag tgtcgtaact
     1621 ctactataca agatttattc ctttttgtgt gtttacacaa gatattttac actaccatca
     1681 aaaatcttaa aaggtattta taaaagcatg aatggtatca tttaaacttc ttaattaatt
     1741 aactattgtc acagcgtaag tgtagagagg ataaaaagac atttcatgat gaaaaatagc
     1801 cttttaagat tttgaaacca tgatactgtt ctttatgata ttctgtacta gctcgaggtt
     1861 gcaacgagac aaatttaaga attatgaaaa gaagttattc gatatggaga taaagaaact
     1921 ggaaacattt caccaaatga ctatagaaaa gcttgccaag gttgagggtg gtaagaataa
     1981 ttggcaggca aatgtcagtg gggttatcgc tgcaggtagt gctggagctg ctataggttt
     2041 tccagtttgc ggtgtagcct gtggttatat tggagctaaa acagctatta ctctttgggc
     2101 tggtgtaaca ggagctacag gcggcttcta gaggaaagaa aggatgacaa caatgaagga
     2161 attaacaatt aatgacatgg ctagtataag tggaggaaac gctccaggag atgctgtaat
     2221 tggtggtctt ggaggcttag cttctggatt gaaattttgt aaactgccac atccagtatt
     2281 ggcaggtgga tgtgttgtag gatttacagt tggtggagca tatttgggat atacagcaaa
     2341 ttaaatgatc tatacgattt ttattattat gaatgcgtct ataatgtttt taatcattgt
     2401 tgatgccatt cataaaaaaa tgttgataaa tctataccat atttagtgtt tttaattttc
     2461 aatcaattat ccctttcgga aaaatcggta aacatgttat ctatcgtagc tttggtagcg
     2521 tcattatttg tttttatcga taaaagaaaa tagtgattat gccaatgaat ttagatatca
     2581 ctcttagctt tttaaaatac aaaagagtga aattagaatg aaaaagactt caaaaatatg
     2641 gaagtgctaa aaaaatggtc tgactatcat aaatggaggc ggaaaagacg gagcaaatat
     2701 ctttttgaca gggatggcag gtgctgcgca gggagtgaca gtttgtgctc agacaggcgt
     2761 atttatccct tggcaaggtt atattttgtg tggagcagct ggtgctgcta caaatattat
     2821 ctggcctcac taaatatata aatgactatc tatcactgat atataagtag tagaggagtg
     2881 gtatgaaaat tttagatatt atttttaacc tatatgatag aaagatgata tttctagaaa
     2941 atgttgaaaa gctgggaggt tacattgtcc cttttaccct tatcatcttg agtgtagcct
     3001 acaactttag gcacccttat cctatttggg ctgctgtact aggaggtata tcccttattg
     3061 tgatctttat tt
//
LOCUS       DATXQK010000092         3071 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.092, whole genome shotgun sequence.
ACCESSION   DATXQK010000092 DATXQK010000000
VERSION     DATXQK010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.092"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            27..881
                     /locus_tag="VC394_001709"
     CDS             27..881
                     /locus_tag="VC394_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HEP5995150.1"
                     /translation="MKSKKVVSVISLTLSLFLVIGCAKVDNNKSVNCKPATKQTYNSY
                     SDDQLRSRENTMSVLWYQRAAETQALYLQGYQLATDRLKEQLNKPTDKPYSIVLDIDE
                     TVLDNSPYQAKNVLEGTGFTPESWDYWVQKKEAKPVAGAKDFLQFADQNGVQIYYISD
                     RSTTQVDATMENLQKEGIPVQGRDHLLFLEKGVKSKESRRQKVKETTNVTMLFGDNLL
                     DFADFSKKSQEDRTALLSDLQEEFGRRFIIFPNPMYGSWEGAIYKGEKLDVLKQLEER
                     RKSLKSFK"
     gene            1074..2270
                     /locus_tag="VC394_001710"
     CDS             1074..2270
                     /locus_tag="VC394_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEP5995151.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKEGKSLAPVAADLAAKLGQDVVFPGVTRGSKLEE
                     AINALEDGQVLLVENTRFEDVDGKKESKNDEELGKYWASLGDGIFVNDAFGTVHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKDLLEKSNGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            2533..>3071
                     /locus_tag="VC394_001711"
     CDS             2533..>3071
                     /locus_tag="VC394_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEP5995152.1"
                     /translation="MKTLRKLLSNYKFDIKKFKLGMRTLKTGLSVFLVLLVFHLFGWK
                     GLQIGALTAVFSLREDFDKSVHFGFSRIIGNSIGGLLSLVFFAFNEIFHQAFWVTLLI
                     VPICTMLCIMVNVACNNKSGIIGAVAALLIITLSIPTGQTFIYVTSRVFETFCGVFVA
                     ILVNTDVELIKNKWFNKKH"
BASE COUNT          996 a          474 c          622 g          979 t
ORIGIN      
        1 cttaataagc aaaaaggaga ttttcaatga aatccaaaaa agttgttagt gttatatcac
       61 ttaccttatc cctttttttg gtgataggtt gtgctaaagt tgataacaac aaatcagtta
      121 attgtaagcc tgctactaaa caaacctata atagctatag tgatgaccaa ttaagatcgc
      181 gtgaaaatac catgtctgtt ttatggtacc agcgagctgc ggaaactcaa gcgctttatt
      241 tacaggggta tcagttagca acggatcgct taaaagaaca actcaataaa ccaacggata
      301 aaccttattc aattgtatta gatattgacg aaacagttct tgataatagc ccttatcaag
      361 ctaaaaatgt tttggaagga acaggattta cacctgaaag ctgggattat tgggtacaaa
      421 agaaagaagc aaaaccggtt gctggtgcta aagacttttt gcaatttgca gaccaaaatg
      481 gtgttcaaat ttactacatt tcagacagat caactactca agtagatgct acaatggaaa
      541 atctccaaaa agaaggtatt ccagtacaag gtcgtgatca tcttctattc ttagaaaaag
      601 gcgtaaaatc aaaggagagt cgtcgtcaaa aggtcaaaga aacaactaat gtaacgatgc
      661 tatttggtga taatcttcta gattttgctg atttttcaaa aaaatctcaa gaagatagaa
      721 cagctttatt atcagatttg caagaagagt ttggaagacg ctttatcatt ttccctaatc
      781 ctatgtatgg ttcatgggaa ggtgccattt ataaaggtga aaagctggat gtgcttaagc
      841 aactagagga acgccgtaaa agtttaaaaa gctttaaata aagtaattga aaaagaggtg
      901 caaaagaaaa tgtaacctct ttttaagtac tattattttc tgaaaatagg gttaatgact
      961 gaaaaaaatg ttgattttgt gaaatgatta ttttattatt ttcatatttt atctttatgc
     1021 taggaattgt ggtataatgg aggagtataa aaaatattaa ggagtctatt cacatggcta
     1081 aattgactgt taaagacgtt gatttgaaag gtaaaaaagt cctcgttcgt gttgacttta
     1141 atgtgccttt gaaagatggc gttatcacta acgacaaccg tatcactgcg gctcttccaa
     1201 caatcaagta tatcatcgaa caaggtggtc gtgctatcct cttctctcac cttggacgtg
     1261 ttaaagaaga agctgacaaa gaaggaaaat cacttgcacc ggtagctgct gatttagctg
     1321 ctaaacttgg tcaagatgtt gtattcccag gtgttactcg tggttcaaaa ttagaagaag
     1381 caatcaatgc tttggaagat ggacaagttc ttttggttga aaacactcgt tttgaagatg
     1441 ttgacggtaa gaaagaatct aagaatgacg aagaacttgg taaatactgg gcttcacttg
     1501 gagatggaat cttcgttaac gatgcatttg gtacagtaca ccgtgctcac gcatcaaacg
     1561 taggtatttc agcaaacgtt gaaaaagctg tagctggttt ccttcttgaa aacgaaattg
     1621 cttacatcca agaagcagtt gaaactccag aacgtccatt cgtagctatt cttggtggct
     1681 caaaagtttc tgataagatt ggtgttatcg aaaatcttct tgaaaaagct gataaagttc
     1741 ttatcggtgg tggtatgact tacacattct acaaagctca aggtatcgaa atcggtaact
     1801 cacttgtaga agaagacaaa ttggatgttg ctaaagacct ccttgaaaaa tcaaatggta
     1861 aattgatctt gccagttgac tcgaaagaag caaacgcatt tgctggttac actgaagttc
     1921 gcgacactga aggtgaagca gtttcagaag gcttccttgg tcttgacatc ggtcctaaat
     1981 caatcgctaa atttgatgaa gcacttactg gtgctaaaac agttgtttgg aacggaccta
     2041 tgggtgtctt tgaaaaccct gacttccaag ctggtacaat cggtgtcatg gacgctatcg
     2101 ttaaacaacc aggcgttaaa tccatcatcg gtggtggtga ttcagcagca gctgctatca
     2161 accttggccg tgctgacaaa ttctcatgga tcagtaccgg cggaggcgct agcatggaac
     2221 ttctcgaggg taaagtatta ccaggtttgg cagcattgac tgaaaaataa tagtcattat
     2281 aatatatagt aaagggcatg ctgatgcatg tcttttttta gttgatcatg tacagttatt
     2341 gaactttccc taacttcact tatcatttac tagtttttag atatagtggt atttggctga
     2401 cactgcaatt ttcaaaagtt tatgacgatt tcatatcaat tggtttataa aagatatcct
     2461 aatcataaat aaaacgttta ccaaaagggt tttatggatt gatatgttaa aatagatagt
     2521 gaagggatag gtatgaaaac gttaagaaaa ttattatcta attacaagtt tgatattaaa
     2581 aagtttaaat tgggaatgag aacattgaaa acaggcttat cggtcttttt agttttattg
     2641 gtttttcatt tatttggttg gaaagggctt caaattggtg ctctaactgc tgtatttagt
     2701 ttaagagaag actttgataa aagcgtgcac tttggctttt ctagaattat tggtaatagt
     2761 ataggtggcc tactctcctt agtatttttt gcattcaatg agatatttca tcaagctttt
     2821 tgggttactt tattaattgt tcctatttgt accatgttat gtatcatggt taatgttgca
     2881 tgtaataata aatcaggtat tattggggca gtggctgctc ttttgattat tacactgtct
     2941 attccaacag gacaaacgtt tatttatgtg acttcgcggg tatttgaaac attttgtggc
     3001 gtctttgtag ctattttagt taatactgat gttgaactta ttaaaaataa atggtttaac
     3061 aaaaaacaca g
//
LOCUS       DATXQK010000093         2984 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.093, whole genome shotgun sequence.
ACCESSION   DATXQK010000093 DATXQK010000000
VERSION     DATXQK010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.093"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..1475
                     /locus_tag="VC394_001712"
     CDS             <1..1475
                     /locus_tag="VC394_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4135 domain-containing protein"
                     /protein_id="HEP5995153.1"
                     /translation="FENQLFETIMFLSMKTLVLDINHFSKEIENKSEAYEQCIQQIRE
                     ENGINHFFDRYPYLLKQINKEVGLIEESYSLLFDRFLEDLSEIRSCFNISEPLSNVAF
                     SLGDSHSKKQTVVKIAFKEKSVYYKPKSYHSHSILLELTSLLKSSNIPSFSLPKSLVK
                     ADYCWQLGVAYTSSNKDEVAKIYFKYGVLAAFSEIFSITDLHMENVIVSGGDLYLIDV
                     ETFFQRKLNVQNQNFEGITVDTYQRIYETSLSNGLFPVQFEKNSAPNVSGISGKGGKR
                     KKGKYELINKNRGDMKLVKVDYFQEDGFNIPTLNGKVVEPLDYANEIISGFRECYIFL
                     LSQRSKIKGIVEGFPELKSRALFRNTSDYGKFLQASTNPKYLFSEKKKKNLFSILYEA
                     KHIEHFIVDNEIKDLMNGDIPYFSMDTRGNVYNSVGTLIGNLGDTTSLFDSITILNDE
                     RLKFTCELLEIVLKKPIKHWEREKGKSYQFLSISSEHYLL"
     gene            1477..2010
                     /locus_tag="VC394_001713"
     CDS             1477..2010
                     /locus_tag="VC394_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011527761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5995154.1"
                     /translation="MDKTDFRNISAFVSQDSPMFDGDVMYNISLGRESVSGEQVIETC
                     KRVSIYDDIRSMPMKFHTPLFRDNPSLSGGQKQRISLARELVTTPRILVLDEPTSALD
                     VKTERIIQKNVEALHCTRILVTHRLNTVEKADKILIMDNGKIIDYGNHHYLYKNNKDY
                     CDLYDSYMNKYQEEEVK"
     gene            2007..2744
                     /locus_tag="VC394_001714"
     CDS             2007..2744
                     /locus_tag="VC394_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000812013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEP5995155.1"
                     /translation="MKQLVLKDVCKKYPNQLNYALDHINLIVEKGEFVAVMGRSGSGK
                     TTLLNVTSIIDKIDSGSIYCADKEISAFSDNEATSFRKNDIGFVFQDYMLLDSLTIRE
                     NISVALSLKNVDSSKIDDLINNYAKRFNLYEQLKKYPYQLSGGQRQRVSIIRAIIKEP
                     EIIFADEPTGALDLKSSEGTMRILSEINKIEKVTILMVTHDVLSASYADRVVLLKDGK
                     LHMEIDKEDCGETFYDVISQALSDRGE"
     gene            2746..>2984
                     /locus_tag="VC394_001715"
     CDS             2746..>2984
                     /locus_tag="VC394_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP5995156.1"
                     /translation="MIWSITKSNIKKNFSLYRIYFLATIGLLSIFIAFLNFISDKIIT
                     EKIGDSGQALVIANGSLVFLTVFLVIFLIYFNNFFV"
BASE COUNT         1064 a          385 c          552 g          983 t
ORIGIN      
        1 agtttgaaaa tcagttattt gaaacgatca tgttcctttc gatgaaaaca ctagttttag
       61 atataaatca tttttccaag gaaattgaaa ataagtcaga ggcatatgag cagtgtatac
      121 agcagataag agaagaaaac ggaataaatc atttttttga caggtatccg tatttgctaa
      181 aacaaattaa taaagaagta ggactgatag aggaatcata ttctttatta tttgatagat
      241 ttttagaaga cttatctgag ataaggtcgt gctttaacat tagtgagccc ctaagcaatg
      301 ttgccttttc acttggtgac agtcactcta aaaagcaaac agttgttaaa attgcgttta
      361 aagagaaatc tgtttactac aagcctaaaa gttatcattc acacagtatc ttgttagaac
      421 ttactagttt attgaaatct agtaatatac cttcattcag tcttcctaaa agcttagtta
      481 aagcagatta ttgttggcaa cttggtgtag cttatacaag ttccaataaa gatgaagtag
      541 cgaaaattta ctttaagtat ggtgttttag cagcattttc tgaaatattt tctattacgg
      601 acttacatat ggaaaatgtg attgtttcag gcggagattt atatttaatt gacgttgaaa
      661 cattttttca aagaaagttg aatgtacaaa atcagaattt tgaagggatt actgtagaca
      721 cctatcagag aatttatgag acatcacttt caaacggttt atttcctgta caatttgaga
      781 aaaacagcgc tcctaacgtt tctggaataa gtggaaaagg tggaaaaaga aaaaaaggaa
      841 aatatgaact gataaataaa aatcgtggag atatgaagtt agtaaaagtt gactattttc
      901 aagaagatgg gttcaatatt cccacattaa atgggaaagt ggtagagcct cttgactatg
      961 ctaatgagat catttcagga tttagagagt gctacatatt tcttctatca caacgttcaa
     1021 agattaaagg aatagtagaa ggttttccag aattgaaaag cagagcccta tttcgaaata
     1081 cttccgatta tggaaaattt ttgcaagctt ctacgaatcc aaagtatctt ttctcagaaa
     1141 aaaagaagaa gaatttattt tcgatactat atgaggcaaa acatattgaa cattttattg
     1201 ttgataatga aattaaagat ttgatgaatg gtgatattcc ttacttttca atggatacca
     1261 gaggaaacgt ctataattct gtagggacat taattggaaa tctaggagat acaacctcgt
     1321 tatttgatag tataactatt ttgaacgatg aaagattgaa gttcacttgt gaattgcttg
     1381 aaatagtctt gaaaaaaccg attaagcatt gggagaggga aaagggcaaa tcttatcagt
     1441 ttttatcaat ctcctcagaa cattaccttc tctaaaattg ataaaactga ttttaggaat
     1501 atatcggctt ttgtatctca agattctccc atgtttgatg gggatgtgat gtataacatt
     1561 tcgctaggga gagaatctgt ttcaggagaa caggttattg aaacttgtaa aagggtatca
     1621 atatatgatg atatcaggag tatgccaatg aagtttcata ccccactttt tcgagataat
     1681 ccatcactat ctggggggca aaaacaacga atttctttag caagagaact tgtaaccacc
     1741 cctagaatct tagttcttga cgaacctaca tcagctttag atgtaaaaac tgaaagaata
     1801 atccaaaaaa atgttgaggc tttacattgt acgaggattt tggttaccca tagacttaat
     1861 acagttgaaa aagctgataa gattttaata atggataatg gcaaaattat tgactatgga
     1921 aaccatcatt atttgtacaa aaataataag gattattgtg acttatatga ctcgtatatg
     1981 aataaatatc aggaggaaga ggtaaaatga aacaattagt attaaaagat gtttgcaaaa
     2041 aatatcccaa tcaactgaat tatgctctag accatatcaa tttgattgtg gaaaaaggag
     2101 aatttgtagc tgtcatgggg cgtagtggta gtggaaaaac aacactttta aatgttactt
     2161 caattattga taaaattgat agtgggagta tttattgtgc agacaaagaa attagtgcat
     2221 tttctgataa cgaggcaact agttttagaa aaaatgatat tggttttgtt tttcaagatt
     2281 atatgctgtt agacagtcta accattaggg agaatatttc tgttgctcta tctttaaaaa
     2341 atgttgattc atcaaaaatt gatgatttga taaataacta tgcaaaaaga tttaatttat
     2401 atgagcagtt gaaaaaatat ccgtatcaac tttctggagg tcaaagacag agggtcagta
     2461 ttatcagagc aatcattaaa gaaccagaga ttatttttgc tgacgaacca actggagcgc
     2521 ttgacttaaa atcttctgaa ggaacaatga ggattttgag tgaaatcaat aaaatagaaa
     2581 aggtaactat tttaatggtt actcatgacg ttttatcagc ttcttatgcg gatagagttg
     2641 tacttctcaa agatggtaaa cttcacatgg agattgataa agaggattgt ggagagacgt
     2701 tttatgatgt aattagtcaa gccttatccg atagaggaga gtaatatgat ttggtccatt
     2761 acaaaatcta acatcaaaaa aaacttttcg ttatatcgta tctattttct agctacgatt
     2821 ggtttattaa gtatttttat agcttttcta aattttatct cagataaaat cattacagaa
     2881 aaaattgggg atagtggtca agctctagtt attgctaacg ggtcattagt ttttttgact
     2941 gtatttttgg tgatattttt aatttatttc aataatttct ttgt
//
LOCUS       DATXQK010000094         2795 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.094, whole genome shotgun sequence.
ACCESSION   DATXQK010000094 DATXQK010000000
VERSION     DATXQK010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.094"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            100..333
                     /locus_tag="VC394_001716"
                     /pseudo
     CDS             100..333
                     /locus_tag="VC394_001716"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            361..588
                     /locus_tag="VC394_001717"
                     /pseudo
     CDS             361..588
                     /locus_tag="VC394_001717"
                     /inference="COORDINATES: protein motif:HMM:NF025063.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            715..870
                     /locus_tag="VC394_001718"
                     /pseudo
     CDS             715..870
                     /locus_tag="VC394_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039767.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(917..1447)
                     /locus_tag="VC394_001719"
     CDS             complement(917..1447)
                     /locus_tag="VC394_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HEP5995157.1"
                     /translation="MSNFISPVYEIKSIPIEKISPNDYNPNSVAPPEMKLLYDSIKSD
                     GYTMPIVCYYDKEEDRYSIVDGFHRYRIMLDYSDIYERESGRLPVSVIDKSLDYRMAS
                     TIRHNRARGSHDVDLMSQIVKDLHECGRSDNWIAKHLGMDKDEILRLKQITGLASLFK
                     DHEFNQSWEPEELLNE"
     gene            complement(1440..2774)
                     /locus_tag="VC394_001720"
     CDS             complement(1440..2774)
                     /locus_tag="VC394_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HEP5995158.1"
                     /translation="MKKYLNQNVYDALIERLHFLFNEFPIVYISFSGGKDSGLLLNIL
                     LDFRDKYYPDREIGVFHQDFEAQYSLTTKYVQETFTSLEGRKKVSLYWVCLPMATRTA
                     LSSYEMFWYPWDDKTEDIWVRPMPSQDYVINLENNSITTYRYKMNQEDLAKQFGRWYK
                     QIHGNQKTVCILGNRASESLHRYSGFINKKYGYQKECWITKQFKDVWTASPLYDWSVE
                     DIWHAYYKFSYSYNELYDLFYKAGLKPSQMRVASPFQDYAVDSLNLYRIIDQETWVKL
                     LGRVQGVNFSNIYGRTKAMGYKSIALPKGHSWKSYTQFLLSTLPVRLRNNYVRKFNKS
                     IDFWHKTGGGLAEETINELIEKGYRIARNGISNYTSFKHSRVIFLDQIPDDTDDIVTT
                     KDIPSWKRMCFCILKNDHICRTMGFGLTREQQKIVAFLKEKYKTSLKELESE"
BASE COUNT          917 a          561 c          391 g          926 t
ORIGIN      
        1 ctctatgcta aaggaagtcg atatcaatat aaatactata accgtcgacg ttcttcatga
       61 actcgtccca atcttgttaa tcaatgtttc caagcgactg ataagaataa attatggcta
      121 ggagacttaa cctacattcc tacacaagaa ggaatacttt atgtgtctgt cttgatagac
      181 gtttatagta gaaagattgt tggttgggca atgggccgac gtatgcaaga caagttggta
      241 acagaagctt tcaatcaagc ttataataga gaaaagccta aagaaggggt aatcgtccat
      301 acagatcagg gttctcaata taccggagca tgatttcagg acctacttag acagaaaaaa
      361 tgtaagtcca ggatgagtcg aaaaggtaat ccatacgata atgcactcat ggaatccttt
      421 tataaaacat taaaaagaga gcttgtcaat gatgctcatt tcgcaactat tgagcaagct
      481 cagcttgaga tttttaaata tagtgagact tactataacc ctaaacgtct gcattcagct
      541 ttagggtatc tctcacctgt agaatttgaa aaaatagtta ctcattagct taactttatg
      601 tctatttttt cttgacaagt ccagggtatg aagtcccctt atacacatac tttcaaaaca
      661 gctctcaatc ctctgtccgc tacaaaaata cgtcaccgct gccctccagc tgcctcattc
      721 aacgacaaac ttgaagccac aaacagactc atcaagggca ttaagtacaa tgcctttaga
      781 tttcagaact ttgacaactt taaaaccaaa atcctcatcg ctttgaacat ccaaaaagag
      841 agaaccaatc tgattctctc tcgttgttaa tgataagtca cccactacag ttgacaatga
      901 gtctcttact tcctgtttac tcatttaata gctcttctgg ttcccaagat tgattaaact
      961 catggtcctt aaaaagagaa gccaatcctg ttatttgttt cagtcttaaa atttcatctt
     1021 tatccattcc taaatgtttt gcaatccagt tatcacttcg cccacactca tgaagatcct
     1081 ttacaatctg actcatcaaa tcaacatcat gacttcctct agctctatta tgtcgaatgg
     1141 tagaagccat tcgatagtct aatgacttat caataacaga aacaggtaat ctcccacttt
     1201 ccctttcata aatatctgaa taatctagca ttatacgata tctatgaaaa ccatcaacaa
     1261 tactataccg atcctcttcc ttatcgtaat agcaaacaat cggcatagta taaccatcag
     1321 attttatact atcatacaat aatttcattt caggcggagc aactgaattc ggattataat
     1381 catttggaga aatcttttca attggaattg atttaatctc atagactgga ctaataaaat
     1441 tactcacttt ctaactcctt taaactcgtc ttatatttct cttttaaaaa ggctactatt
     1501 ttttgttgct ctcttgtcaa accaaacccc atagttcgac aaatatgatc gttttttaaa
     1561 atacagaaac acattcgttt ccagcttggt atatcctttg ttgttacaat atcgtctgta
     1621 tcatcaggaa tttgatctaa aaaaataacc cttgaatgtt taaaagaggt ataatttgaa
     1681 ataccatttc tagcaatacg atatcctttt tcaattagtt cattaattgt ttcctccgca
     1741 agtcctccgc ctgttttatg ccaaaagtct attgatttat taaattttct aacataatta
     1801 tttcttaacc tcactggtag cgttgataaa agaaactgag tgtacgactt ccacgaatga
     1861 ccctttggta aagcaattga tttatatccc attgctttag tacgtccata aatattcgaa
     1921 aagttaactc cttgaacacg tcccaaaagc tttacccaag tttcttgatc tattattcta
     1981 taaagattta ggctatcaac agcatagtct tgaaaaggag atgcaactct catttgtgat
     2041 ggttttaaac ccgctttata gaatagatca taaagttcgt tataagaata agaaaactta
     2101 tagtaagcat gccatatgtc ttccactgac caatcatata agggagatgc tgtccagaca
     2161 tctttgaatt gtttggtaat ccagcattcc ttttgataac catacttttt attgataaaa
     2221 ccactatatc tgtgtaagga ttctgaagca cggtttccta atatacaaac tgttttttga
     2281 tttccgtgaa tttgtttata ccacctacca aattgtttcg ctaaatcctc ctgattcatt
     2341 ttataacgat aagtagtaat tgaattattt tccaaattaa taacataatc ttgagatggc
     2401 attggtctta cccagatatc ttctgttttg tcatcccacg gataccaaaa catttcataa
     2461 ctactaagtg cagttctagt agccatcggt aagcacaccc aatacaaaga aacttttttt
     2521 ctaccttcta aagaagtgaa tgtttcttga acatattttg ttgttaaact gtactgtgct
     2581 tcaaaatctt gatgaaacac acctatttct ctatccggat aatatttatc tctaaaatcc
     2641 agtaaaatat taagaagtaa cccactatct tttccacctg agaaggaaat atagacaata
     2701 ggaaattcat taaacaaaaa atgtaatctt tctatcaatg catcataaac attttgattt
     2761 aaatactttt tcactaaact tcctcattat gcatt
//
LOCUS       DATXQK010000095         2665 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.095, whole genome shotgun sequence.
ACCESSION   DATXQK010000095 DATXQK010000000
VERSION     DATXQK010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.095"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..673)
                     /locus_tag="VC394_001721"
     CDS             complement(<1..673)
                     /locus_tag="VC394_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronidase"
                     /protein_id="HEP5995159.1"
                     /translation="MSRDPTLLIDESNLTIGSDGRAYYTFTADGDTKSVKIANNKCIG
                     TTRFNQLMIERGGKPTNYVAPVVVEGTGNPTGLFKDLKEISLELTDTKNSKLWSKIKL
                     NNQGMIEEYYNGTIKSEIIKTAEGTQQRISSETDKKLALINETVSGIRREYQDADRQL
                     SSSYQAGIEGLKATMANDKIGLQAEIQTTAQGLYQRYDNEIRKLSAKITTTSSGTTEA
                     YESKLD"
     gene            complement(684..1013)
                     /locus_tag="VC394_001722"
     CDS             complement(684..1013)
                     /locus_tag="VC394_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5995160.1"
                     /translation="MRGHNLLKDLNNIIINNKTIDRIICNNTIVYQRSWDLLFDGLLT
                     SAGKKLDIYPFYKIVINNAFFVKTPTTKNNTIFLAGHKILALQDSHFCFFRALEKSFF
                     VKIYGKN"
     gene            complement(1000..2214)
                     /locus_tag="VC394_001723"
     CDS             complement(1000..2214)
                     /locus_tag="VC394_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5995161.1"
                     /translation="MTSVETIPIKIVFDRKDASEWQSTNPVIDEGELVVELDTHKLKV
                     GDGKTSYNDLPYYEGPQGESITKVQLSENGDLSVWIGDKETKLGNIKGQKGDKGTSIT
                     DITKDGETLTIKLSDDTQKIFNIPNGQKGDRGKGVESARIDESGHLKLKIEEESELDL
                     GNVKGESGPKGDSITITNHKRVSDGTQVSFSDGTQIVVPKGDTGDVNGINLEDYVKKS
                     ELKNVGSADVKAINDFLGLSQKVFTSSYSYTDSLLKSYAKPSYSASWYVNESTVSTKN
                     GDKVLITIHNTTTQADNYLEVAVTYVGANYVTATSTGRLLTTPGEVKVVTKKQAEKDY
                     AAKKHKHEISDIAGLNERLSGYLRQADIQSQLNNIGKLKDTQTGQYLEVKVVDKGQVP
                     SNTSGMIVFERS"
     gene            complement(2211..>2665)
                     /locus_tag="VC394_001724"
     CDS             complement(2211..>2665)
                     /locus_tag="VC394_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002988790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEP5995162.1"
                     /translation="NLLVGDPNRVKTYDQDYIEVEANDGGFKRGETYTISFSQTCELL
                     KKVAITLTQINNKGVKLVLTPTKAKMDAQTFEVTKDKQSIEVYPLSYTGVLTGDWYKS
                     KSVEINASEAQELALEMAYKEIADAKGATIIGKQSDKPKIILDGRRDR"
BASE COUNT          661 a          575 c          480 g          949 t
ORIGIN      
        1 catcgagctt gctttcatag gcctctgtag tgcctgacga ggtagtggtt attttagcgg
       61 acagcttgcg tatctcgtta tcataccttt gatacaagcc ttgagcggtt gtttgtatct
      121 cagcttgtaa accgatttta tcattggcca ttgtggcttt tagcccctca ataccagctt
      181 ggtagcttga cgatagctgt ctatctgcat cttggtactc acgtctaatg cctgagactg
      241 tctcgttgat aagcgcaagt tttttatcgg tctcgctact aatacgttgc tgcgtgccct
      301 ctgcggtttt gataatctca gattttattg taccgttgta atactcttca atcatccctt
      361 gattattaag cttgattttt gaccaaagtt tggagttttt agtatctgtt aactcgaggc
      421 taatctcttt gaggtcttta aatagtccag tcggattacc tgtcccctcg acaaccacgg
      481 gcgccacgta gttagttggt ttaccccctc gctcaatcat gagctggtta aagcgagttg
      541 taccgataca tttgttattg gctattttaa cgctttttgt gtcaccatca gccgtaaatg
      601 tataataagc acgtccatct gagccgattg ttaaatttga ctcgtctatt aaaagtgttg
      661 ggtctctact caaatgttac ctcctaattt ttaccgtaaa ttttgacaaa aaaacttttt
      721 tctaatgctc taaaaaaaca aaaatgacta tcttgtaatg ctagtatttt atgccctgct
      781 aaaaaaatcg tattgttttt tgttgtgggg gttttaacaa aaaatgcatt attgattacg
      841 attttataaa aaggataaat atccaatttt ttcccagcgc ttgtcagcaa accgtcaaaa
      901 agcagatccc acgaacgctg atagacaatc gtattattac aaataatacg atcaatcgtt
      961 ttgttattaa taatgatatt atttaaatct tttaacaaat tatgacctct caaacacgat
     1021 catgccactg gtattgctag gcacctgtcc tttatcaacc actttaacct caagatattg
     1081 tccggtttgc gtgtctttta gcttaccgat attattaagc tgtgactgta tatcagcttg
     1141 tctgagatag ccagacaagc gctcattaag accagctatg tcactaatct catgcttatg
     1201 ttttttagca gcataatctt tttccgcttg cttttttgtc actactttga cttcaccagg
     1261 agtagtcagt aagcgacctg ttgaggtagc agtcacgtag ttagcaccaa cataagtcac
     1321 cgctacctcc aaataattgt ctgcttgggt ggtagtgtta tgtattgtaa tcaatacttt
     1381 gtcaccattt ttggtagaaa ctgtagattc gttgacatac caactcgccg aatagctggg
     1441 ttttgcataa ctttttagta agctatctgt ataactatag ctgcttgtaa acaccttttg
     1501 agatagccct aaaaagtcat taatagcttt aacatctgca gaaccgacgt ttttaagttc
     1561 agattttttg acataatctt ccagattgat gccgttgaca tcaccagtat ccccttttgg
     1621 gacaacaatt tgtgtcccgt cgctgaaaga tacctgcgtt ccatctgaaa cacgcttgtg
     1681 atttgtgata gtaatactat cacctttagg tcctgactca cctttaacgt ttccaagatc
     1741 taactctgat tcctcttcaa tttttaattt taaatgaccg gattcgtcaa tcctagcgct
     1801 ctctacgccc ttacctctat cccctttttg gccattgggg atattaaaga ttttttgagt
     1861 gtcgtctgat agtttgatag tgagtgtctc accatctttg gtgatgtcgg ttatacttgt
     1921 ccctttgtcc cctttttgac ctttgatgtt gccaagctta gtctctttgt cgccaatcca
     1981 cacagacaag tcaccatttt cggatagttg tacttttgtt atggattctc cttgcggacc
     2041 ttcatagtaa ggcaagtcat tgtagcttgt tttaccatct ccgaccttta gcttatgggt
     2101 gtctagctcg acgactagtt caccttcgtc aatgacaggg ttagttgact gccattctga
     2161 agcgtctttt cggtcaaaga caatttttat tggtattgtt tcgactgatg tcatctgtcc
     2221 ctcctcccat ctaaaataat ttttggttta tcggactgtt tgccgataat agttgcacct
     2281 ttggcatctg caatctcttt ataagccatc tcaagagcta attcctgcgc ctctgaggcg
     2341 tttatctcca cagacttaga tttataccaa tcacctgtta aaacgcctgt atagctcaaa
     2401 ggataaacct ctatagactg tttatcttta gtgacctcaa acgtctgtgc atccattttt
     2461 gctttggttg gtgtcagtac taacttaact cccttgttgt taatttgcgt caatgtgata
     2521 gctacttttt tgagtagctc acacgtttga ctaaagctaa tcgtgtaggt ctcgccacgc
     2581 ttaaagccgc catcattagc ttctacctcg atatagtctt ggtcataggt ttttacacgg
     2641 ttagggtcgc caaccaataa atttt
//
LOCUS       DATXQK010000096         2598 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.096, whole genome shotgun sequence.
ACCESSION   DATXQK010000096 DATXQK010000000
VERSION     DATXQK010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2598
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.096"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            153..566
                     /gene="yaaA"
                     /locus_tag="VC394_001725"
     CDS             153..566
                     /gene="yaaA"
                     /locus_tag="VC394_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEP5995163.1"
                     /translation="MIYKLFTEFITLQALLKELGIIQSGGAIKGFLAETTVLFNGEDE
                     KRRGKKIRIGDKISLPDQDLIITIVEPSQEEKEQFAEEIAEKTRVAALVKQMNQANKK
                     TSSKHKNRQSTTKKSLRATKKTKGKPTAPVRFPGI"
     gene            568..1674
                     /gene="recF"
                     /locus_tag="VC394_001726"
     CDS             568..1674
                     /gene="recF"
                     /locus_tag="VC394_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEP5995164.1"
                     /translation="MWIKELELKHYRNYDHLLASFSSGLNVFIGNNAQGKTNFLEAIY
                     FLSLTRSHRTRADKELIHFDHSTVSLTGKIQRISGTVDLEINLSDKGRVTKINALKQA
                     KLSDYIGTMMVVLFAPEDLQLVKGAPSLRRKFIDIDLGQIKPVYLSELSHYNHVLKQR
                     NSYLKSAQQIDAAFLAVLDEQLAGYGARVMEHRIDFINALEKEANTHHQAISNGLESL
                     SLSYQSSVVFDKKTNIYQQFLHQLEKNHQKDFFRKNTSVGPHRDNLAFYINGMNANFA
                     SQGQHRSLILSLKMAEVSLMKALTGDNPILLLDDVMSELDNTRQTKLLETVIKENVQT
                     FITTTSLDHLSQLPEGIRIFHVTKGTVQVDSDIH"
     gene            complement(1731..2594)
                     /locus_tag="VC394_001727"
     CDS             complement(1731..2594)
                     /locus_tag="VC394_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HEP5995165.1"
                     /translation="MEGIFYALIPMFTWGSIGFVSNKIGGKPSQQTLGMTFGALLFSL
                     AVWLIVRPEMTLQLWLFGILGGFIWSIGQTGQFHAMQYMGVSVANPLSSGSQLVLGSL
                     IGVLVFHEWTRPMQFVVGSLALLLLIIGFYFSSKQDDANAQVNHLHNFSKGFRALTYS
                     TIGYVMYAVLFNNIMKFEVLSVILPMAVGMVLGAITFMSFKISIDQYVIKNSVVGLLW
                     GIGNIFMLLAASKAGLAIAFSFSQLGAIISIVGGILFLGETKTKKEMRWVVTGIICFI
                     VGAILLGVVKS"
BASE COUNT          919 a          503 c          432 g          744 t
ORIGIN      
        1 agtacagctt ttgttaaaac taattaacaa gaacttcaaa actaaatagt tatttggacg
       61 agtatgatac tttataaata gctcttattc agctattatt atttatttct gttttatgct
      121 ataatgactt gaatgacaaa atggagatga caatgatata caaattattt acagaattta
      181 tcaccctaca agcccttctt aaagaattag gaattatcca aagtggtggc gctatcaagg
      241 gatttctcgc tgaaacaacc gttctattta atggtgaaga tgagaaacgt cgaggcaaaa
      301 aaatcagaat aggggataag atttctctac ctgaccaaga cttaattatc actattgttg
      361 agcctagtca agaagaaaaa gaacaatttg ccgaagaaat agctgaaaag acacgtgtag
      421 cggctctagt gaaacaaatg aaccaagcta acaaaaagac ttcttctaaa cataaaaatc
      481 gtcaatctac taccaaaaaa tcactaagag caactaagaa aactaaaggt aaaccaactg
      541 cacctgtccg cttcccaggt atctagtatg tggattaaag agttagagtt aaaacattat
      601 cgcaattatg accacctgct tgcctcattt tcgtcgggtt taaatgtctt tatcggtaat
      661 aatgctcaag gaaaaaccaa ttttctcgag gcaatctatt ttctatcact tactagaagt
      721 catcgcacga gggctgataa agaattgatt cattttgatc actcaactgt ttctcttact
      781 ggtaagattc agcgtattag tggtactgtt gatttagaaa tcaatctatc agataaagga
      841 cgtgtgacaa agattaatgc gctaaagcaa gcaaaattat ctgattatat tggaacgatg
      901 atggttgtgc tttttgcacc agaagatttg caactagtta agggagcacc tagccttcgt
      961 cgcaaattta tcgacattga ccttggtcaa attaagcctg tttacttatc tgagttatcc
     1021 cattataacc acgtgcttaa acagcgtaat agctatttaa aaagtgccca acaaattgat
     1081 gccgcttttc tagctgttct agatgagcaa ttagctggct atggagcccg tgtcatggaa
     1141 catcgtattg attttatcaa tgcgttagag aaagaagcaa atactcatca ccaagccatc
     1201 tcaaatggtt tagaaagtct ctctctctcc tatcagtcgt cagttgtctt tgacaaaaaa
     1261 actaatattt accaacaatt tttacaccaa cttgaaaaaa atcatcagaa agatttcttc
     1321 cgtaaaaaca ctagcgttgg tccacatcgc gataacttag ccttttacat taacgggatg
     1381 aatgctaact ttgctagtca aggtcagcac cgcagcctta ttctttcctt aaaaatggct
     1441 gaagttagtt taatgaaggc cttgactgga gataacccca ttcttttact agacgatgtc
     1501 atgagcgagc tagacaatac acgacaaaca aagttacttg aaacagtaat taaagaaaat
     1561 gtccaaacct ttatcacaac aacaagtttg gatcacctgt cacagttgcc agaaggtatc
     1621 cgtatttttc acgtaacaaa aggaaccgtt caagttgatt ctgatattca ctaataacat
     1681 caaaaaaagg gttcagttaa actgagcctt tttttgatat tatgtggcga ttaagatttg
     1741 accacaccta ataaaatagc acctacgata aaacaaataa ttcctgtgac aacccaacgc
     1801 atttcttttt tggttttggt ttcgcctagg aataaaatgc ctccaacaat cgaaatgata
     1861 gcacctaatt gtgagaaact aaaggcaata gcaagtcctg cttttgatgc agctaaaagc
     1921 atgaaaatat tcccaatgcc ccaaagtaat ccaacaacgc tatttttaat cacatactgg
     1981 tcaatggaaa ttttaaatga catgaaagtt atagctccta aaaccatacc tactgccatg
     2041 ggcaaaatga ctgacaaaac ttcaaatttc atgatgttat taaataatac agcatacatc
     2101 acgtaaccaa ttgttgaata agtgagcgcc ctaaatcctt ttgaaaaatt atgaaggtga
     2161 ttgacttgtg cattagcatc atcttgttta ctagaaaagt aaaaaccaat aatcagaagt
     2221 aatagtgcta gacttcctac tacaaattgc ataggtcttg tccattcatg aaaaacgagg
     2281 acaccaatca aacttcccag aacaagttga gagccacttg acaaaggatt ggcaacggag
     2341 actcccatgt attgcatggc atgaaattga ccagtttgac caattgacca gataaaacca
     2401 cccaatattc caaaaagcca aagttgcagt gtcatctcag gtctaacaat taaccaaaca
     2461 gctaatgaaa ataataaagc cccaaaagtc attcctagag tttgttgcga aggttttcct
     2521 ccaattttgt tactaacaaa tccaatgctt ccccatgtaa acatgggaat aagtgcataa
     2581 aaaatacctt ccaaaatg
//
LOCUS       DATXQK010000097         2464 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.097, whole genome shotgun sequence.
ACCESSION   DATXQK010000097 DATXQK010000000
VERSION     DATXQK010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.097"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..236
                     /locus_tag="VC394_001728"
     CDS             <1..236
                     /locus_tag="VC394_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002987674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEP5995166.1"
                     /translation="ILPTYNLVKSYVDFEKQNQQVVKLKKEYNELSKRTKKEKQLAER
                     LKDDNFVKKYARAKYYLSREGEMIYPIPGLLPK"
     gene            371..1657
                     /locus_tag="VC394_001729"
     CDS             371..1657
                     /locus_tag="VC394_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HEP5995167.1"
                     /translation="MRKLLAAMLMTFFLTPLPVISTEKKLIFSKNAVYQLKQDVVQST
                     QFYNQLPSNPNLYQETCAYKDSDLTLPAGRLGVNQPLLIKSLVLNKESLPVFELADGT
                     YVEANRQLIYDDIVLNQVDIDSYFWTQKKLRLYSAPYVLGTQTIPSSFSFAQKVHATQ
                     MAQTNHGTYYLIDDKGWASQEDLVQFDNRMLKVQEMLLQKYNNPNYSIFVKQLNTQTS
                     AGINADKKMYAASISKLAPLYIVQKQLQKKKLAENKTLTYTKDVNHFYGDYDPLGSGK
                     ISKIADNKDYRVEDLLKAVAQQSDNVATNILGYYLCHQYDKAFRSEIKALSGIDWDME
                     QRLLTSRSAANMMEAIYHQKGQIISYLSNTEFDQQRITKNITVPVAHKIGDAYDYKHD
                     VAIVYGNTPFILSIFTNKSTYEDITAIADDVYGILK"
     gene            1654..>2464
                     /gene="tilS"
                     /locus_tag="VC394_001730"
     CDS             1654..>2464
                     /gene="tilS"
                     /locus_tag="VC394_001730"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEP5995168.1"
                     /translation="MMTYQEIFNEIKNKAYFKNHRHVLIAVSGGVDSMNLLHFLYLFQ
                     DKLKIRIGIAHVNHKQRSESDSEEAYLKCWAKKHDIPIYVSNFEGIFSEKAARDWRYA
                     FFKSIMLKNNYSALVTAHHSDDQAETILMRLIRGSRLRHLSGIKSVQPFANGQLIRPF
                     LTFSKKDLPEIFHFEDSSNRELSFLRNRVRNNYLPLLKQENPRFIQGLNQLALENSLL
                     FQAFKELTNHITTTDLTEFNEQSKSIQYFLLQDYLEGFPDLDLKKSQFTQLL"
BASE COUNT          922 a          362 c          386 g          794 t
ORIGIN      
        1 ttattttgcc aacttataat cttgtcaaaa gttatgttga ttttgaaaag caaaatcaac
       61 aggtggttaa attaaaaaaa gagtataatg aattgtcaaa gaggacaaaa aaagaaaaac
      121 aattagcaga acgactaaaa gatgataatt ttgtcaaaaa atatgctagg gcaaaatact
      181 atttatcgcg tgaaggagaa atgatttatc ctattccagg actattacca aaatgatgat
      241 ggataatatt gtaaaaaaaa tagaagcatt tcttgctttt tctgataaaa aattagcaga
      301 gctgcaacag gaaaatcaaa aagttaaaga agaaagtcag tatattcaaa aaaactagaa
      361 aggagtcaat atgagaaaat tattagcggc tatgttaatg actttttttc tgactccttt
      421 accagtgatt agtacagaaa aaaaacttat attttcaaaa aatgctgttt atcaattgaa
      481 acaagatgtc gttcaatcaa cacaattcta taatcagcta ccctctaatc caaatcttta
      541 tcaagaaacg tgtgcctata aagacagtga tttaactcta ccagcaggaa gattaggtgt
      601 aaatcaacca ttacttatta aatcgcttgt tcttaacaaa gaatctttac cggtttttga
      661 gttagctgat ggtacctatg ttgaggctaa tcgacaattg atttatgacg atattgtact
      721 taatcaagta gatatagata gctatttttg gacacaaaag aaacttaggc tttattcagc
      781 cccttatgtt ttaggtacgc aaacaattcc ttcttctttt tcatttgctc aaaaagttca
      841 tgccactcaa atggcacaaa caaaccatgg aacttattat cttattgatg ataagggctg
      901 ggcatcacaa gaagatctag ttcaatttga taaccgcatg ttaaaagtcc aggaaatgct
      961 cttacaaaaa tataataacc caaattattc aatttttgta aagcaactca acacacaaac
     1021 aagtgctggt attaatgctg ataaaaaaat gtatgctgca agtatctcga agttagcacc
     1081 actttatatt gttcaaaaac aattacaaaa aaagaaatta gcagagaata aaactttgac
     1141 ttatactaaa gatgttaatc atttttatgg agactatgat ccattgggaa gtggtaaaat
     1201 tagtaaaata gctgataata aagattatcg tgttgaagac ctactgaaag ctgtagcaca
     1261 acaatcggat aatgtagcaa ctaatatttt aggttattat ctatgtcatc agtatgataa
     1321 agctttccgc tcagagataa aagctttatc aggtatcgat tgggatatgg agcagcgctt
     1381 attaacttct cgttcagctg caaatatgat ggaagctatt tatcatcaaa aaggccaaat
     1441 tatttcttac ctttcaaata ccgaatttga tcaacaacgt atcacaaaaa atattactgt
     1501 tccagttgca cataaaattg gtgatgctta tgattataaa catgacgttg ctattgttta
     1561 cggtaatact ccatttattt tgtctatttt tacaaataaa tcaacctatg aagatattac
     1621 ggctattgca gatgacgttt atggtatttt aaaatgatga cctatcaaga aatttttaat
     1681 gaaataaaaa ataaagccta ctttaaaaat catagacatg tgttaatagc cgtatctggt
     1741 ggagtagatt caatgaattt attgcacttt ttatacctgt ttcaagacaa attgaaaatt
     1801 cgtattggta ttgctcatgt taatcacaaa caaagatcag aatcagattc agaagaagct
     1861 tatttaaagt gttgggctaa aaaacatgac atacctattt atgtttctaa ttttgaagga
     1921 atattttcag aaaaagcagc acgtgattgg cgctatgctt tttttaaaag tattatgtta
     1981 aaaaataact attcagcttt agtaacagct caccattcag atgatcaagc agaaactatt
     2041 ttgatgaggc taattagagg aagccgattg aggcacttgt cgggtataaa aagcgtgcaa
     2101 ccatttgcca atggtcaact tataagacct tttttaactt tctcaaaaaa ggacttgcca
     2161 gaaatttttc attttgaaga ttcttcaaat agggaattat catttttaag aaatagagtt
     2221 agaaataatt atttaccatt gttaaagcag gaaaatccta ggttcattca gggattaaat
     2281 caattagcac ttgaaaatag cttacttttt caagctttta aggagctaac aaatcatatt
     2341 actaccactg atttaacaga atttaatgag caatctaagt ctattcaata ctttttgtta
     2401 caagattatt tagaaggttt tccagattta gatttaaaaa aatctcaatt tactcaatta
     2461 ttgc
//
LOCUS       DATXQK010000098         2446 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.098, whole genome shotgun sequence.
ACCESSION   DATXQK010000098 DATXQK010000000
VERSION     DATXQK010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.098"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            28..897
                     /locus_tag="VC394_001731"
     CDS             28..897
                     /locus_tag="VC394_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HEP5995169.1"
                     /translation="MYHTTALSFLKKGYQVIPLRKDTGTPMIKFKDIQITEEVIKNTN
                     WFNCDYALLMRGIWCIDIDTHDMDEKLAKELYIMIKKMGIDLLSVLSTDKYDNGLDGY
                     SSIIRHEYKNELISNFKNTFAELTASGGMHILFKKRDGINYTQKIGVMPGVDIKANDN
                     NFVKIFPSDGREVLQAVKTLPYYDGKFEEEAFKPKQEGITTYFGGSVTYTSNGSHEGR
                     EAYERVATGTSYNRNNDLFKGACWAFENGIDIDDLTSIIGTVKGRDVFTREEFELTIE
                     SAKRKVSYVTIRT"
     gene            851..1201
                     /locus_tag="VC394_001732"
     CDS             851..1201
                     /locus_tag="VC394_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HEP5995170.1"
                     /translation="MNQRNEKSATSLSEHDIQNLIRMELSQAGHMVFRANVGKVKTAD
                     GRFFDTGLPKGFCDLFGFKPNGQIFFIEVKNETGRIRPEQKKFMEVMASRGALVGVAR
                     SVEDALNIVNDTSR"
     gene            1185..1541
                     /locus_tag="VC394_001733"
     CDS             1185..1541
                     /locus_tag="VC394_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5995171.1"
                     /translation="MTLVDDFYKQMEPSIKAFLDDNITIADKEEADRVYRSVKYYKKL
                     NRLPPPDVLEWFQRIYTTKEMIMLIKQSYRLKQKKTDEDDKIYEKWMFKNYGDVKLVK
                     KIKRTDALERARRMGL"
     gene            1783..2019
                     /locus_tag="VC394_001734"
     CDS             1783..2019
                     /locus_tag="VC394_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HEP5995172.1"
                     /translation="MIEELVHKPKHYNHGEIDLIESWYKTYPWDQFVAIMESHIDKYI
                     KRHRYKGNATQDLEKALEYTKRLIEYWLIESVEE"
     gene            2016..2186
                     /locus_tag="VC394_001735"
     CDS             2016..2186
                     /locus_tag="VC394_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002995952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5995173.1"
                     /translation="MKRYIEVKDEWKSATDHLNDFIDKNKYAKVTVVGYQVVQLSPYG
                     RDLTYILAEVEE"
     gene            2183..>2446
                     /locus_tag="VC394_001736"
     CDS             2183..>2446
                     /locus_tag="VC394_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5995174.1"
                     /translation="MTIDEALQNLRDNFNKIMNVLKNDWKALLFLAIAIFGMMVTVSY
                     FSYRDARQYYESQITGLRTQLSRTQKQLKRASEDRARQTKRIAE"
BASE COUNT          916 a          360 c          527 g          643 t
ORIGIN      
        1 gtagtaaaaa agggggtgcg ttaccttatg taccacacaa cagcactttc attcttaaaa
       61 aaaggatatc aggttatacc gcttagaaaa gatactggaa cgccaatgat taagtttaaa
      121 gatatccaaa taacggaaga agtgatcaaa aatacaaact ggtttaattg tgattatgct
      181 ttattaatgc gtggcatttg gtgtattgat attgatactc atgacatgga cgagaagtta
      241 gctaaagagt tatacataat gataaaaaag atggggattg atttattatc tgtattatcg
      301 actgataagt atgacaatgg actagatggt tattcgtcaa ttattaggca tgagtataaa
      361 aacgaattaa tcagtaattt taaaaataca ttcgcagaat taactgcaag tggtggtatg
      421 cacatactct ttaaaaaacg cgatggtatt aattacacgc aaaaaatagg agtaatgcct
      481 ggtgttgaca taaaagcgaa tgataataat tttgtcaaaa tattcccatc tgacggacgt
      541 gaagttttac aagcagttaa aacattacct tattatgatg gaaaatttga agaggaagca
      601 tttaaaccaa aacaagaagg tataacaacc tattttggag gttctgtcac atatacatca
      661 aacggaagtc acgaaggccg agaggcttat gaacgtgtag caactggaac atcatataac
      721 aggaataatg atttgtttaa aggagcgtgt tgggcgtttg aaaatggtat cgatattgat
      781 gatttaacat caattattgg gacggttaaa ggcagagacg tatttacgag agaggagttt
      841 gaattaacga ttgaatcagc gaaacgaaaa gtcagctacg tcactatcag aacatgatat
      901 ccaaaacctt atccgaatgg aattatcaca agcggggcat atggtatttc gagcaaatgt
      961 cggaaaggtg aaaacagcag atggtagatt ttttgataca ggtttaccaa aaggtttttg
     1021 tgatttgttt ggatttaaac caaacggaca aatatttttt atcgaagtta aaaatgaaac
     1081 aggtcggata agaccagaac agaaaaaatt tatggaggtc atggcatcta gaggagcgct
     1141 tgtaggagtg gctcggtcag tggaagatgc cttaaacata gtcaatgaca ctagtagatg
     1201 atttttacaa acaaatggag ccgtcaatta aagcgttttt agacgataac attaccatcg
     1261 cagacaaaga agaagctgac agagtctata gatctgtcaa atactataaa aaactaaaca
     1321 gattgccgcc acctgatgta ttggagtggt tccaacgaat ttacacgaca aaggaaatga
     1381 taatgttaat caagcagtct taccgcctta aacaaaaaaa gacagatgag gatgacaaga
     1441 tttacgaaaa gtggatgttt aaaaactacg gtgacgttaa gctcgttaaa aaaatcaaac
     1501 gcacggacgc actagaaaga gctcggagaa tgggtctatg aaaagacaca gacagtggca
     1561 taacgatatt aaatatacac ctagatctta cgataatctg ttgccttacg atatatcaga
     1621 gctgttaata gctcacagat gcaaaataaa gatgtctgat gacgttttag cagataagat
     1681 aggcatttat acttggcaat taaaagcgct cttagaacgc agaatattgc caaatgagag
     1741 cgagtgtaaa ttgattataa attttttgag agaggtggag tgatgataga agaattagta
     1801 cataaaccaa agcattataa tcacggagaa attgatttga ttgagtcgtg gtataagacg
     1861 tacccatggg accagtttgt cgcaatcatg gaaagtcaca ttgataaata cattaaacga
     1921 catagataca aaggcaatgc gactcaggat ttagaaaaag cactcgaata tacaaagcgt
     1981 ctcattgaat attggttaat cgaaagcgtg gaagaatgaa gagatatatt gaagttaagg
     2041 acgaatggaa aagtgcaaca gaccacctga acgattttat cgacaaaaac aagtacgcaa
     2101 aagtgacagt tgttggttat caagtcgtac aactttctcc ttacggaaga gatttgactt
     2161 atattttggc agaggtggaa gaatgacaat agatgaagcg ttgcaaaatt tacgtgataa
     2221 ctttaataaa ataatgaatg tcctaaaaaa cgattggaaa gcactattgt ttcttgcaat
     2281 cgcaatattt gggatgatgg taaccgtgtc gtattttagc tatcgcgacg cacgacaata
     2341 ttacgagtcg caaatcacag gactacgtac acagctaagc aggacacaaa agcagcttaa
     2401 acgtgctagc gaagatagag ctagacagac aaagcgaatt gcggaa
//
LOCUS       DATXQK010000099         2367 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.099, whole genome shotgun sequence.
ACCESSION   DATXQK010000099 DATXQK010000000
VERSION     DATXQK010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.099"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            1..1123
                     /gene="hasA"
                     /locus_tag="VC394_001737"
                     /pseudo
     CDS             1..1123
                     /gene="hasA"
                     /locus_tag="VC394_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018340.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronan synthase HasA"
     gene            1159..2367
                     /gene="hasB"
                     /locus_tag="VC394_001738"
     CDS             1159..2367
                     /gene="hasB"
                     /locus_tag="VC394_001738"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase HasB"
                     /protein_id="HEP5995175.1"
                     /translation="MKIAVAGSGYVGLSLGVLLSLQNEVTIVDILPSKVDKINNGLSP
                     IQDEYIEDYLKSKQLSIKATLDSKAAYKEAELVIIATPTNYNSRINYFDTQHVETVIK
                     EVLSVNSHATLIIKSTIPIGFITEMRQKFQTDRIIFSPEFLRESKALYDNLYPSRIIV
                     SCEENDSPKVKADAEKFALLLKSAAKKNNVPVLIMGASEAEAVKLFDNTYLALRVAYF
                     NELDTYAESRKLNSHMIIQGISHDDRIGMHYNNPSFGYGGYCLPKDTKQLLANYNNIP
                     QTLIEAIVSSNNVRKSYIAKQIINVLEERESPVKVVGVYRLIMKSNSDNFRESAIKDV
                     IDILKSKDIKIIIYEPMLNKLESEDQSVLVNDLENFKKQANIIVTNRYDNELQDVKNK
                     VYSRDIFNRD"
BASE COUNT          824 a          371 c          398 g          774 t
ORIGIN      
        1 aaacttggat tatctttcct ttatgagcca tttaaaggaa atccacatga ctataaagtt
       61 gctgccgtaa ttccttctta ataatgaaga tgccgagtca ttattagaaa ctcttaaaag
      121 tgtgttagca cagacctatc cgttatcaga aatttatatt gttgatgatg ggagttcaaa
      181 cacagatgca atacaattaa ttgaagagta tgtaaataga gaagtggata tttgtcgaaa
      241 cgttatcgtt caccgttccc ttgtcaataa aggaaaacgc catgctcaag cgtgggcatt
      301 tgaaagatct gacgctgacg tttttttaac cgtagattca gatacttata tctatccaaa
      361 tgccttagaa gaactcctaa aaagcttcaa tgataagaca gtttatgctg caacaggaca
      421 tttgaatgct agaaacagac aaactaatct attaacgcga cttacagata tccgttacga
      481 taatgccttt ggggtggagc gtgctgctca atcattaaca ggtaatattt tagtttgctc
      541 aggaccattg agtatttatc gacgtgaagt gattattcct aacttagagc gctataaaaa
      601 tcaaacattc ctaggtttac ctgttagcat tggggatgat cgatgtttaa caaattatgc
      661 tattgattta ggacgcactg tctaccaatc aacagctaga tgtgatactg atgtaccttt
      721 ccaattaaaa agttatttaa agcaacaaaa tcgatggaat aaatcttttt ttagagaatc
      781 tattatttct gttaaaaaaa ttctttctaa tcccatcgtt gccttatgga ctattttcga
      841 agtcgttatg tttatgatgt tgattgtcgc aattgggaat cttttgttta atcaagctat
      901 tcaattagac cttattaaac tttttgcctt tttatccatc atctttatcg ttgctttatg
      961 tcgtaatgtt cattatatgg tcaaacatcc tgctagtttt ttgttatctc ctctgtatgg
     1021 aatattacac ttgtttgtct tacagcccct aaaactttat tctttatgca ccattaaaaa
     1081 tacggaatgg ggaacacgta aaaaggtcac tatttttaaa taatatatgc atcgagtagt
     1141 tagagaagga gtaattttat gaaaatagca gttgctggat caggatatgt tggattatca
     1201 ctaggagttc ttttatcact tcaaaacgaa gtcactattg ttgatattct tccttctaaa
     1261 gttgataaga ttaataatgg cttatcacca attcaagatg aatatattga agattactta
     1321 aaaagtaagc aattatctat taaagcaact ttagatagca aagcagctta taaagaagcg
     1381 gaactggtca ttattgccac acctacaaat tacaacagta gaattaatta ttttgataca
     1441 cagcatgttg aaacagttat caaagaggta ctaagcgtta atagccatgc aactcttatc
     1501 atcaaatcaa caattccaat aggtttcatt actgaaatga gacagaaatt ccaaactgat
     1561 cgtattatct tcagccctga atttttaaga gagtctaaag ctttatatga caacttatat
     1621 ccaagccgaa ttattgtttc ttgtgaagaa aacgattctc caaaagtaaa ggcagacgca
     1681 gaaaaatttg cacttttatt aaagtctgca gctaaaaaaa ataatgtacc agtacttatt
     1741 atgggagctt cagaagctga agcagtaaaa ctatttgaca atacttattt agcgttaagg
     1801 gtagcttatt ttaatgagtt agacacttac gcagaatcga gaaaattaaa tagtcacatg
     1861 attattcaag gaatttctca tgatgatcga ataggaatgc attataataa cccatcattt
     1921 ggttatggag gttattgtct acctaaagat acgaagcaat tattggcaaa ttacaataat
     1981 attccccaaa cgctaattga agctatcgtt tcatcaaata atgtgcgcaa gtcctatatt
     2041 gctaagcaaa ttatcaacgt cttagaagag cgggagtccc cagtaaaagt agtcggggtt
     2101 taccgtttaa ttatgaaaag taactcagat aattttagag aaagtgctat caaagatgtt
     2161 attgacattc ttaaaagtaa agacattaag ataattattt atgagccaat gttaaacaaa
     2221 cttgaatctg aagatcaatc tgtacttgta aatgatttag agaatttcaa gaaacaagca
     2281 aatattatcg taactaatcg ctatgataat gaattacaag atgttaaaaa taaagtttac
     2341 agtagagata tttttaatag agactag
//
LOCUS       DATXQK010000100         2274 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.100, whole genome shotgun sequence.
ACCESSION   DATXQK010000100 DATXQK010000000
VERSION     DATXQK010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.100"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            12..755
                     /locus_tag="VC394_001739"
     CDS             12..755
                     /locus_tag="VC394_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEP5995176.1"
                     /translation="MNILGNSIKEIRKSKKMTQKELAKLTGFKQNTISNHENGNRQLD
                     EVDIRKYAKALKIEPQQLFDYSSSPTNPQVELIPSTLQKINSTSSQLEHSRQIIVLDT
                     AETLLEQQKEIKNNEDTIAELFSYNYYDHAASAGTGQYLNDVQVEKIELPVDYDADFV
                     IPVYGDSMEPKYHSGDYVFVKLSVELTDGDIGVFEYYGDAYIKQLLINAEGAFLHSLN
                     QCGDYPDIPIDRDSDFRIIGEVMGSYRER"
     gene            768..1577
                     /locus_tag="VC394_001740"
     CDS             768..1577
                     /locus_tag="VC394_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEP5995177.1"
                     /translation="MDSKALNKQNLSRSKKILLNISDNPKNPKFYKLSRQELERAKIF
                     KTENLIKSQKYQRYKRGTIVFIHFGVNIGNEFSDSHFGIVLNKKDHPNNGKLTILPLT
                     SKNSKESLSINKEIFTSIMDDAEKTVQTVQNVLNLTTEVERIHHSLPVPPAFFQIKKE
                     NKYHDIWMKYYNRHDPKGIHVPVANITVRKWIQSDLDKINFLKKRYANYDKVSYAKLD
                     SITSVSKLKIAKPINDLDPVGKITLSKEIMDNIDKALARQLLSGQWGKLDN"
     gene            1827..>2274
                     /locus_tag="VC394_001741"
     CDS             1827..>2274
                     /locus_tag="VC394_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HEP5995178.1"
                     /translation="MKIKSYKKENGETAYKFLLYAGYVNGKRKYIRREGFKTKQAARE
                     TLISLQAELDKPKSSMTFGVLTKQWLNEYEKTVQGSTYLKTERNINKHILPKLDKVKI
                     GDINPLLIQRLTEEWCNDLKYGGKILGLVRNILNLAVRYGYINNNPA"
BASE COUNT          860 a          393 c          403 g          618 t
ORIGIN      
        1 ggaaaaatat tatgaatatt ctcggaaatt ctatcaaaga aattcgaaaa tccaaaaaaa
       61 tgacccaaaa agagttagca aaactaactg gatttaaaca aaatacaatt tctaatcacg
      121 aaaatggtaa tagacaactt gacgaagtag atattcgaaa atatgctaaa gcactgaaaa
      181 tagagccaca acagcttttt gactattcct cttctcccac aaacccccaa gttgaactca
      241 tcccatctac cctacaaaaa ataaactcta cttcttctca attagaacac agtagacaga
      301 taattgtttt agatacagct gagactttat tggaacaaca gaaagaaatt aaaaacaacg
      361 aagatactat tgccgaatta ttttcttaca actactacga ccacgcagct tcagctggta
      421 caggtcagta tctaaatgat gtacaagtag aaaaaattga gttaccagtc gattatgacg
      481 cagactttgt tatccctgtt tatggtgatt ccatggaacc gaagtatcac tctggggatt
      541 atgtatttgt taagctatcc gtagagctta cagatggcga tataggcgtc tttgaatact
      601 atggtgacgc ttatatcaaa cagttgctta taaacgctga aggggcgttt ttacacagtt
      661 taaatcaatg cggtgattat ccagatatac cgatagatag agatagcgac tttaggatta
      721 ttggtgaagt tatggggagt tatagggaga gataggcagg agcagaaatg gatagtaaag
      781 cattgaataa acaaaattta agccgtagca agaaaattct gttgaatatc tctgataatc
      841 cgaaaaatcc caaattttac aaattatcaa gacaagaact cgagcgcgct aaaattttta
      901 aaacagaaaa cttaattaaa tctcaaaaat atcagagata caaacgagga actatcgtct
      961 ttattcattt tggagtaaat atcggcaatg aattttccga ttcgcacttt gggattgtgc
     1021 tgaataaaaa agatcatccc aataacggaa agctaactat cttaccgcta acatcaaaaa
     1081 actctaaaga gagtctgtca attaacaaag agatttttac gagcataatg gatgatgccg
     1141 aaaaaacagt tcaaacagtt caaaatgtgc taaacctaac aactgaagta gaacggatac
     1201 accactcgtt accagttccc ccagcgtttt ttcagattaa aaaagaaaat aaatatcatg
     1261 atatatggat gaaatattac aataggcatg accctaaagg gattcatgtg cccgtagcaa
     1321 acattaccgt ccgaaaatgg atacaatcag acttagacaa aattaacttc ctcaaaaaaa
     1381 gatatgcaaa ttatgacaag gtgtcctacg ccaagctgga ctcaatcact tctgtcagta
     1441 aactaaaaat cgctaaacca attaacgatc ttgaccctgt cggaaaaata acattgtcta
     1501 aagaaatcat ggacaacatt gataaagctc tcgcaaggca gttattatct ggccagtggg
     1561 ggaaacttga caattaaata tcactatgtt agaattaagg tgtaatcttg gtagcctcgc
     1621 taccactgaa acattatttt ggagtgtcca actccactgt gagcgcctgt tttcgaataa
     1681 gcgctctttt ttgttttaaa aaaataaaaa agccccacgc tctcaaagtt tggcgactct
     1741 gagcgtgagg caagacagta taagaaacaa ccattaaaaa ggtcattttc ttgtacctat
     1801 tttatcaaat tgaaagatgg tatgcaatga aaattaaatc atataaaaag gaaaatggtg
     1861 aaactgctta taaatttctt ttgtatgccg gttatgttaa tggaaagaga aaatatatta
     1921 ggcgagaagg tttcaaaact aagcaggctg caagggaaac cttaattagt ttgcaagctg
     1981 aacttgataa acctaaatca agtatgacgt ttggtgtgtt aactaaacaa tggctaaacg
     2041 aatatgaaaa aactgtccag ggcagtacct acttaaaaac agaaagaaat attaataaac
     2101 atattttgcc aaaacttgat aaagtgaaga ttggagacat caacccacta cttatccagc
     2161 ggcttactga agaatggtgc aacgatttaa aatatggagg aaaaattctt gggcttgtta
     2221 ggaatatctt aaatctagct gttagatacg gatatatcaa taacaatcca gctt
//
LOCUS       DATXQK010000101         2198 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.101, whole genome shotgun sequence.
ACCESSION   DATXQK010000101 DATXQK010000000
VERSION     DATXQK010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.101"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            178..2010
                     /gene="lepA"
                     /locus_tag="VC394_001742"
     CDS             178..2010
                     /gene="lepA"
                     /locus_tag="VC394_001742"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEP5995179.1"
                     /translation="MNSQDLKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRHEVEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVDAPLQALIFDSVYDAYRGVILQ
                     VRIVNGIVKPGDKIQMMSNGKTFDVTEVGIFTPKAVGRDFLATGDVGYVAASIKTVAD
                     TRVGDTVTLANNPAKEALHGYKQMNPMVFAGIYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVVYHVHTTDEDMIE
                     VSNPSEFPDPTRVAFIEEPYVKAQIMVPQEFVGAVMELSQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYDMSEYRRSQLVKMDILLNGDKVDALSFI
                     VHKEFAYERGKIIVEKLKKIIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKAIGSVEVPQEAFLSVLSMDDDAKK"
BASE COUNT          645 a          393 c          525 g          635 t
ORIGIN      
        1 agaggctaag cagatgattg atttaaaaag gaatcttttc aaaattgatg tccgggcaca
       61 aaaggatgaa gagaaagttt tcatgcgtac ggcgtggata atccgtaaga aaagataggc
      121 aaaggcctac cttttttgtt ataatagaag gaaagactaa atgtatagga aaatagaatg
      181 aacagtcaag atttaaaaaa acgtcaggag aagattcgta atttctccat tattgcacat
      241 attgaccatg gcaaatctac tctcgctgac cgtattttgg aaaagacaga gacggtttcg
      301 tctcgtgaaa tgcaggctca gttattggac tccatggatt tggagcgtga gcgtgggatt
      361 accatcaagt taaacgccat tgagcttaat tacacggcta aagatgggga aacctacatt
      421 ttccacctta ttgatacccc agggcatgtg gactttactt atgaagtatc gcgttcgttg
      481 gcagcctgtg agggagcgat tctagttgtg gatgcagcgc agggcattga agcacagacc
      541 cttgccaatg tttacctagc ccttgataat gatttggaaa ttttaccggt tatcaataaa
      601 attgacttgc ctgctgctga ccctgagaga gtccgccatg aagtagaaga tgtcattgga
      661 cttgatgctt ctgaggctgt tctggcgtca gccaaggctg gtattgggat cgaagagatt
      721 cttgagcaaa ttgttgagaa ggttcctgct cctacgggtg atgtggatgc gcctttacag
      781 gccttgattt ttgactctgt gtatgatgct taccgagggg taattttgca ggttcggatt
      841 gtgaacggta ttgtcaagcc tggcgataaa atccagatga tgtcgaatgg aaaaaccttt
      901 gatgttacag aagttgggat tttcactcct aaggcagttg gacgagattt ccttgcgacg
      961 ggagatgttg gttatgttgc ggcctctatc aaaacggtag cagatacccg tgtgggcgat
     1021 acggtgactt tagctaataa ccctgctaag gaagccttgc atggctacaa gcagatgaac
     1081 ccgatggtct ttgcagggat ttatccgatt gaatcaaata aatacaatga cttgcgtgaa
     1141 gcgcttgaaa aattacaact gaacgatgcc agtttacagt ttgaacccga aacgtcacaa
     1201 gcgcttggct ttggttttcg atgcggtttt ttaggcttgc ttcacatgga tgtgattcaa
     1261 gaacgtttgg aacgtgaatt taacattgat ttgatcatga cagcaccgtc cgtggtgtat
     1321 cacgttcaca caacagatga ggacatgatt gaagtgtcta acccttcaga atttccagat
     1381 ccaacacggg tggcttttat tgaagagcct tatgttaaag cgcaaatcat ggtgccacaa
     1441 gagttcgtag gggctgttat ggaattatcg cagcgcaagc gtggtgattt tgtgaccatg
     1501 gattacattg atgataaccg tgtcaatgtg atctaccaaa tcccgctggc tgaaattgtc
     1561 tttgatttct ttgataaatt gaaatcctca acgcgaggct atgcgagttt tgattatgac
     1621 atgtcagaat accgcaggtc acaattggtg aaaatggaca ttcttttgaa cggtgataag
     1681 gtcgatgcgc tcagctttat tgttcataaa gaatttgctt atgaacgcgg gaaaattatt
     1741 gttgagaaat tgaaaaaaat cattccacgt caacagtttg aagtgcctat tcaagccgct
     1801 attggtcaaa aaattgttgc gcgttcagac atcaaggccc ttcgtaaaaa cgttttagcg
     1861 aagtgttatg gtggtgacgt gtctcgtaaa cgtaaattgc tcgaaaaaca aaaggctggt
     1921 aaaaaacgga tgaaggctat tggttctgtt gaagtccctc aagaagcctt cttgagtgtt
     1981 ctttcaatgg atgacgatgc caagaaataa ccaagcctaa tcgcttagtc ttcagaggag
     2041 aactcttgtg ggtctacatg accaaaaaat ggttatctta tacaaaaaga actttacaat
     2101 catttgcaca taatgttata ataatattat agtgttatct cattattagg tgcgcctgca
     2161 tttaatctta ttaaacagaa agaggaaaca aaaactgt
//
LOCUS       DATXQK010000102         2189 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.102, whole genome shotgun sequence.
ACCESSION   DATXQK010000102 DATXQK010000000
VERSION     DATXQK010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.102"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            207..380
                     /locus_tag="VC394_001743"
     CDS             207..380
                     /locus_tag="VC394_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004829396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5995180.1"
                     /translation="MNKRQELIDELIKADQDGTYKTYKSTEEIKAMNNEEIQIIYSNM
                     KNYLSDKRTHTNC"
     gene            564..1091
                     /locus_tag="VC394_001744"
     CDS             564..1091
                     /locus_tag="VC394_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HEP5995181.1"
                     /translation="MINEEISKEAGQAAQTIISYTIKAAKESINLEKEIRKKMNDTLE
                     KANGNLKSLMGDEIKIKDLYKKGQLENISIDQSNLKDLKKELNKLGVSFSVMKNKESQ
                     NYEVFFQAKDIKVMEYAFKQVIAKENKKEKESILKQIKKYKDLSKNKNKTKEKVKRKV
                     KEKVKPNKKDMTREI"
     gene            1217..1945
                     /locus_tag="VC394_001745"
     CDS             1217..1945
                     /locus_tag="VC394_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEP5995182.1"
                     /translation="MATNKRYYWIKLKEEFFTDKRIKRLRRISGGDTYTIIYLKLLLL
                     SLKDEGKLYYDGVESDFIKELALTIDETDDDVMVTVNYLINQGLLEVVTENDEYYLTE
                     IPNLIGSETAWAEKKRRYRQNKQRTLSLMSPTHVRQEIEIEKDIEIDKEREGEIDKKS
                     TPIFYGEFKNVRLSKEEYKNLKEKLNSHTEIMINKLSRYMESSGKTYQNHYATILNWY
                     DKDKDKLRQKSLNKKMNYDVGESL"
BASE COUNT         1087 a          301 c          363 g          438 t
ORIGIN      
        1 aatagataag ctaataaaac aaggagaaaa accaagtgta atagcacaga ttaaaaaatt
       61 ccaagaccaa gtcaataaag ataatgaata cagagaaagt atgaaaaaca agaaaatgga
      121 tcaagaaaga taagacctgg taaaaatatc ttatccaagc tataatatga ccaagaaaat
      181 aaagaccgtt ctaggaggta atagagatga ataaaaggca agagctaata gatgaactca
      241 taaaagcaga tcaagatgga acatacaaaa cctacaaaag cacagaagaa ataaaagcaa
      301 tgaacaatga agaaatacag attatatatt ccaacatgaa aaactatcta tcagacaaaa
      361 gaacacacac aaactgctga aggtggaaag gtattgtaag ctatttaaaa gtataaaagg
      421 tacaaacacc taaacaagat atgaaaacat caaaataaag cattccacca ccaaaaacaa
      481 taaattaaaa acaagcaaag ggaagtatag aaaaattaaa acctatactt cccttttttt
      541 aattcaaaca aaggagagaa aacatgataa acgaagaaat aagcaaagaa gcaggtcaag
      601 cagcacaaac cataatatca tacacaataa aggcagcaaa agaatcaatc aacttagaaa
      661 aagaaataag aaaaaagatg aatgatactt tagaaaaagc aaatggaaac ctaaaaagtc
      721 ttatgggcga tgaaataaaa ataaaagacc tctacaaaaa aggacaacta gaaaacataa
      781 gcatagatca aagcaacctc aaagacttaa aaaaagaact aaacaaactt ggagtaagct
      841 tttcagtaat gaaaaacaaa gaaagccaaa actatgaagt atttttccaa gccaaagaca
      901 taaaagtaat ggaatatgcc tttaagcaag tcatagccaa agaaaataaa aaagaaaaag
      961 aaagtatcct aaaacaaata aagaaataca aagacctatc caagaacaag aataagacga
     1021 aagagaaagt aaaaaggaaa gtaaaagaaa aagtaaaacc aaacaaaaaa gatatgacca
     1081 gagaaatcta agggagtaac aaaaagttac acccttaaat aaccataata acaagactta
     1141 cgaaaagcag aagtccagaa ttccgaaaat cggaaatcaa gaatttcgat tatcgaaaat
     1201 caagaaagga gcagatatgg caacaaacaa aagatattat tggataaaac taaaagaaga
     1261 atttttcaca gacaaaagga taaaaagact aaggagaata tcgggaggag atacctacac
     1321 gataatctac ctaaaacttt tattactaag tctaaaagat gagggcaaac tctattatga
     1381 cggagtagaa agtgatttta taaaagagtt agcactaacc atagatgaaa cagacgatga
     1441 tgtaatggtc acagtaaatt atttaatcaa tcaaggctta ctagaagttg taacagaaaa
     1501 tgatgaatac tatttaactg aaataccaaa cttaatcgga tcagaaacag catgggcaga
     1561 gaaaaaaaga agatacagac aaaataaaca gaggacattg tccttaatga gtccaaccca
     1621 tgtccgacaa gagatagaga tagagaaaga tatagagata gataaagaga gagagggaga
     1681 gatagataaa aagtccaccc ccatttttta tggagaattc aaaaatgtaa ggttaagcaa
     1741 agaagaatat aaaaatctta aagaaaaact aaactcacac acagaaataa tgataaataa
     1801 actatcaaga tacatggaaa gcagtggtaa gacctatcaa aaccactatg cgacaatcct
     1861 aaattggtat gacaaagata aagacaaact aagacagaaa agtttaaata aaaaaatgaa
     1921 ttatgatgta ggagaatctt tatagataaa aagaggtgga aagccactat taaagacttt
     1981 aagtctaaaa aggtatcaaa agtatgccag agataataaa aatgtaaata tgacacttaa
     2041 aaggcgatta gaaaattaaa gaaatgctag aagattttta gatgacagtt accaagaggg
     2101 tctaattttc actgctactc atgcagtggg aattggacct ttttacataa gtaaattcta
     2161 ttcaaaatta gttaccaaga aagacttct
//
LOCUS       DATXQK010000103         2118 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.103, whole genome shotgun sequence.
ACCESSION   DATXQK010000103 DATXQK010000000
VERSION     DATXQK010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.103"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..204
                     /locus_tag="VC394_001746"
     CDS             <1..204
                     /locus_tag="VC394_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEP5995183.1"
                     /translation="INGVVVHGDSLTRQVKNIYFLQNTKDDMLSFSDINVMPRTQDIE
                     QEFNVKEWIGDAIVHVESKLNAR"
     gene            209..688
                     /locus_tag="VC394_001747"
     CDS             209..688
                     /locus_tag="VC394_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011888686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEP5995184.1"
                     /translation="MNIEEAIKKIRELEAFNYFDFKGNLIRRIDVLGIIDQLKIIVQQ
                     CVADWYEKHKDNLEYDLYLYQMSIYDEKVEEDDFYYWMQKSKNPVRTLINMHQFGYEI
                     EQEQLYTVDLPNGQPLVRGKNTLYFSQNLTAENAKLTESEIRKDFEWAWQFAKEVTE"
     gene            1137..1571
                     /locus_tag="VC394_001748"
     CDS             1137..1571
                     /locus_tag="VC394_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HEP5995185.1"
                     /translation="MTFFPEINIQKTKSNAKRKLREYPRWRRIANDVDTQKVTATYSF
                     EPRQPHGVPSKPVERLALNRVSAEQELDAIEQAVSMILEPERRRILYDKYLAPYKKAD
                     KVIYTELCMSESFYYDTLDIALLAFAELYREGVLLVEQGVFS"
BASE COUNT          713 a          353 c          453 g          599 t
ORIGIN      
        1 ataaatggtg tggtggtgca tggtgactct ttaacaagac aagttaaaaa catttatttt
       61 ttgcaaaata caaaagacga catgctgagt tttagtgata ttaacgttat gccaaggact
      121 caagatattg agcaagaatt taatgtcaaa gagtggattg gtgatgcgat agtgcatgta
      181 gagagtaagt taaatgcgag gtaacaaaat gaacattgaa gaagctatta aaaagataag
      241 agaactagag gcgtttaatt atttcgactt taaaggtaat ttaattagaa gaattgatgt
      301 attaggtatc atcgaccaac taaaaattat agtacaacaa tgtgttgcag attggtacga
      361 aaagcataaa gataatttgg aatatgattt atatttatat caaatgtcaa tctatgatga
      421 aaaagttgaa gaagatgatt tttattattg gatgcaaaaa tcaaagaatc cagttcgtac
      481 tcttattaat atgcaccaat ttggctatga aatcgagcaa gagcagctgt atacagttga
      541 tttgccaaac ggtcagcctt tagtacgcgg taaaaacact ttgtatttta gtcaaaatct
      601 aacagccgaa aatgcaaaac tcactgaatc agaaatccga aaagacttcg agtgggcttg
      661 gcaatttgcg aaagaggtga ctgaatgact gaagagttag gagtgttata tagcgaaaaa
      721 tggcataagt attatttata taaaacttgc aggtatatat cttatgttga taatccacat
      781 caggctacta aatgcacccg caaacaagca gaacagtttc cacagtttaa atgggtatcg
      841 ctgacaaaat tataacccca cgcaagcgct caagagcctg cgatggctct gtgggggtgg
      901 accgaaatta aaaaatagaa acgagaacct ccttacacca aaacaaatct aaagcgggtt
      961 atcggtcatc cgtgattatc caaggcgtcg ctaatgcttt aacacgacat cgtgcgcctg
     1021 tgtcaaaaaa caaaggaaag agagggcttt tctccacaaa acaaaaagac gtccacacgg
     1081 aacgccccct tggttaaatt taagcttaaa taaattatac cacatcgggg gctttcatga
     1141 cgttttttcc agaaattaat atacaaaaaa ctaaatcaaa cgctaagcga aaactaagag
     1201 agtatccacg ctggcgaagg atagctaatg acgtagatac tcaaaaagtt acagctactt
     1261 actcatttga gccaaggcaa ccgcatgggg tccctagtaa accagttgag agactagcgc
     1321 taaaccgtgt gtcagcagaa caagagctgg atgcgattga gcaagccgtc agtatgatac
     1381 tagagccaga gagacgcagg attttgtatg acaaatattt agcgccttat aaaaaggcag
     1441 ataaggttat ttatacagaa ttgtgtatgt cagagagctt ttactatgat acacttgaca
     1501 ttgctttgtt agcttttgca gagctgtaca gagagggtgt gttgcttgtt gagcaaggag
     1561 tttttagcta gtttttatac agtaataaga tagtttatac atatttttac atgttattat
     1621 agtactatca aaataacaag aagagataac cttttaatca ctgactattt tatttagtcg
     1681 ccaactttaa ctacgatcaa acttgttatt ttatggtatg tgggacgtgc aggttcgaat
     1741 cctgccgtct ctatcagcgt caggggagac gcgaccccac acccatttaa aggtagacac
     1801 gatctgatgt gtggggtttt atgattaacc gcaagtaaaa caagggtcgc aaccttgctt
     1861 gtggttagta ggcttaatgg tcgtttatgc acgttcgatt cgtgcaaagc ctgttaggaa
     1921 catgaaccgt gattggaaaa cggtggaggt agcgccttgc ttcgggacgt tagctaagtc
     1981 cgaaaactct ttgcgaagag ccagaagaag atgtgtcggt tcgattccga ctgttcctgt
     2041 tgcatttaat gcaaactcca tatttttatt aaaaaagcct attataccta ttgtgtaagg
     2101 gcttttttcg ttgtatta
//
LOCUS       DATXQK010000104         1824 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.104, whole genome shotgun sequence.
ACCESSION   DATXQK010000104 DATXQK010000000
VERSION     DATXQK010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.104"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(282..1817)
                     /locus_tag="VC394_001749"
     CDS             complement(282..1817)
                     /locus_tag="VC394_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017284843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEP5995186.1"
                     /translation="MSLFLSLFVSWYLTWFLQEANPLFNKLDRLKRLAKFLFENGFVY
                     EKRKKVNKKQKVSFPKVYLKQGKFDLSVSFEMAGNKFQKRFKDIGGELEDTFFMDFME
                     KTDDPRFKIYKLAYSAFLARITVNDVVWEKDKGIKLMNGYYWDFINDPHLLVAGGTGG
                     GKTVLLRSILKCLATIGVADICDPKRADFVTMADLPAFRGRIVFEKVDIIAKFENAIT
                     IMYARYDFIRKEMKRLGHKDMGKFYDYGLEPYFFICDEYNALMSSLSYQERDIVENAF
                     TQYILLGRQVGCNAIIAMQKPSADDLPTKIRSNMMHHISVGRLDDGGYMMLFGDENRN
                     KEFRYIKFLSGHRVYGRGYSAVFGEVAREFYAPLLTKKFSFYDEFAKISRHDNPFDPL
                     ENTLVSKDIISDESLRKFVEKTSDNELPKGSIGFDFEEEMEEINHMSIGDLAKKTGFS
                     FGKIKNLIDMIEKDGYMTFNREDERVVLTDSDAVILNSLLTQKDKFDGSWKELLLNYF
                     KGE"
BASE COUNT          628 a          391 c          224 g          581 t
ORIGIN      
        1 agctattttt tgataaaata taacctgttt ttgctcgttt tataacctat ttttgaaact
       61 tgaaaatcct tgatacatat ggggtttcgg gggttttact tcgtagattt ttcaaatctt
      121 acccccctat tagaaatcgg gggttttaaa attgataaat ccctctgtaa agccctagtg
      181 tgtgcgctgt gtccggcgtg tccgtttccg ctacgctaca ctacgcctac agctacacta
      241 gggctttaca gaaggatatt taaaaaaata cccttcatct actattcacc cttaaaataa
      301 ttcaacaaca actctttcca actgccatca aacttatctt tttgagtgag taaactattc
      361 aatataactg catcagaatc agtcaatact actctttcat cttcacgatt aaaagtcatg
      421 taaccatctt tttcaatcat atcaattaaa ttttttattt taccaaatga aaaacctgtt
      481 ttcttagcta aatcaccaat actcatatga ttaatttcct ccatttcctc ttcaaaatca
      541 aagccaatac ttcctttagg taactcattg tcagacgttt tttctacaaa ttttctcaat
      601 gattcatctg aaataatatc cttggaaacc aaagtatttt caagcggatc aaatggatta
      661 tcatgacgtg agattttcgc aaattcatca taaaatgaaa atttcttagt tagcaaagga
      721 gcataaaact ctctcgctac ctcgccaaaa acagcagaat agccacgacc atacacacgg
      781 tgacctgata gaaatttaat atacctgaac tctttattcc gattttcatc cccaaaaagc
      841 atcatataac caccatcatc aaggcgacca acagaaatat ggtgcatcat atttgacctg
      901 atttttgttg gtaaatcatc tgcggatggc ttttgcatgg caataatcgc attacagcct
      961 acttgacggc caagtagaat atactgtgta aaggcattct caacaatgtc tctttcctga
     1021 tacgataacg aagacatcaa ggcattatat tcatcacaaa taaaaaaata tggttctaat
     1081 ccgtaatcat aaaatttccc catatcttta tgccctaaac gtttcatttc cttacgaata
     1141 aagtcatagc gtgcatacat gattgtaatt gcattctcaa atttagcaat aatatctact
     1201 ttttcaaaaa caattcttcc tcgaaaagca ggtaaatcag ccattgtcac aaaatcagct
     1261 cgcttaggat cacatatatc cgcaacgcca atagttgcaa gacattttaa aattgatcta
     1321 agcaaaacag tcttaccgcc accagtccca cccgcaacaa gcaaatgagg atcattgata
     1381 aaatcccaat aataaccatt catcagctta atgcctttat ccttttccca tacaacatca
     1441 ttaaccgtta tacgagccag aaaggcggaa taagctaatt tataaatctt aaacctagga
     1501 tcatcagttt tttccataaa atccataaaa aaagtatctt caagttcacc tccaatatct
     1561 ttgaaacgct tttgaaattt gtttcctgcc atttcaaagg agacacttaa atcaaactta
     1621 ccttgtttta aataaacttt tggaaaacta actttctgct tcttattaac ttttttacgt
     1681 ttttcataaa caaaaccatt ttcaaataaa aatttagcca aacgctttaa acgatctaac
     1741 ttattaaata atggattagc ttcttgtaaa aaccaagtca aataccaaga aacaaaaagc
     1801 gataaaaaca aacttatccc taaa
//
LOCUS       DATXQK010000105         1786 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.105, whole genome shotgun sequence.
ACCESSION   DATXQK010000105 DATXQK010000000
VERSION     DATXQK010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.105"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..1022)
                     /locus_tag="VC394_001750"
     CDS             complement(<1..1022)
                     /locus_tag="VC394_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEP5995187.1"
                     /translation="MNKLKKEILSDNYNHFFHFFAVFTGIFVIMTIIILQIMRFGVYS
                     SVDSSLVSVSNNASSYANRTMARISSFYFDTENNIIKALPDSDSSKLLGTPAANTDII
                     LFSANGTILNAFDAFSNYQNFHLDKRRLGSIETTSLMNFYGQEEKYHTITVRVHIKNY
                     PAVAYMMAVVNVEQLDRANERYERIIIIVMSVFWLISILASIYLAKWSRKPILESYEK
                     QKMFVENASHELRTPLAVLQNRLESLFRKPNETILENSEHLASSLDEVRNMRILTTNL
                     LNLARRDDGINPQWTHLDTDFFNAIFENYELVAKEYGKIFYFQNQVNRSLRMDKALLK
                     QLITIL"
     gene            complement(1015..1689)
                     /locus_tag="VC394_001751"
     CDS             complement(1015..1689)
                     /locus_tag="VC394_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP5995188.1"
                     /translation="MIKILLVEDDLSLSNSIFDFLDDFADVMQVFDGDEGLYEAESGI
                     YDLILLDLMLPEKNGFQVLKELREKDIKIPVLIMTAKEGLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRTGKFADKNISFGNLVVDLARKEVKVEGKVVELLGKEFDLLVY
                     LLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYISKIRKKLKGTRFVNRLQTLRSVGY
                     ILKNNE"
BASE COUNT          613 a          345 c          221 g          607 t
ORIGIN      
        1 aataaaatcg ttattaattg ttttagtaaa gccttatcca ttcttaacga tctattgact
       61 tggttctgaa aataaaatat ttttccatat tctttagcaa ctagttcata attctcaaaa
      121 atagcattaa aaaaatctgt atctaaatga gtccactgtg gattaatgcc atcatctcgt
      181 cttgctaaat ttaataaatt agttgttaag atgcgcatgt tgcgaacctc gtctaaacta
      241 gaagcgagat gctcactatt ttctaatatc gtttcgttgg gcttacgaaa aagcgattcc
      301 agacgattct gtaagaccgc caaaggggtc cttagttcat gactagcatt ttcaacaaac
      361 attttttgtt tttcatagct ttctaaaata ggttttctgc tccacttggc taaataaata
      421 cttgctaaaa tagaaattag ccaaaaaaca ctcataacta taataataat gcgctcgtaa
      481 cgttcattag cgcggtctaa ttgttccaca tttactactg ccatcatata ggcaactgca
      541 ggataatttt tgatatgaac ccttacagtt atcgtatggt atttttcttc ttgtccataa
      601 aaattcatta aactggtggt ttcaatactc cccaaccggc gtttatctaa atgaaaattt
      661 tgatagttag aaaacgcatc aaaagcattt aaaattgttc cattagcact aaacaaaatg
      721 atatctgtat tagctgcagg cgttcctaat aacttagaac tatctgaatc aggcagcgcc
      781 ttaataatgt tattttcagt atcaaagtaa aaagaagata ttctagccat cgtacgatta
      841 gcatagctac ttgcattatt actaacagaa actaaactac tatcaactga cgaataaacg
      901 ccaaaccgca taatctgtaa gataataata gtcataatga caaaaatacc tgtaaaaacc
      961 gcaaaaaaat gaaaaaagtg gttatagtta tctgataaaa tctctttttt taatttattc
     1021 attgtttttt agaatatacc ctacgcttct taaagtttga agcctgttaa caaaacgggt
     1081 accttttaat tttttcctta ttttagaaat gtaaacttca acaaccgaaa tcgtagtatc
     1141 actatcgaat ccccataaac gatcaaaaat ttgtgtcttt ggtaaaataa cgttttgatt
     1201 ttgcaaaaga tacaccaaca aatcaaattc tttgccaagt aattcaacta ctttcccttc
     1261 aaccttcacc tcttttcgag ctaagtcgac cactaaattg ccaaaactaa tatttttatc
     1321 tgcaaatttc cctgttcttt ttaacagtgc ttgaattcgc atttttaact cttctaaata
     1381 gaacggcttt gtcagataat catcagcacc taattcaaat ccgtgacctt tatcatctaa
     1441 accctcttta gctgtcatga tcaatacagg aattttaata tctttttctc gcaattcttt
     1501 taagacttga aagccatttt tctccggcaa cataaggtct aacaaaatca aatcataaat
     1561 accactttct gcttcgtata aaccttcatc gccatcaaaa acttgcatga cgtctgcaaa
     1621 atcatctaaa aaatcaaaaa tggaatttga taagctcaag tcatcttcta ctaataatat
     1681 ttttatcatg tctctacctc aattgttctt aaataaagta actatcactt catttaactt
     1741 agtctcattt ctgataacaa tttctaacga gactatacta caaaaa
//
LOCUS       DATXQK010000106         1730 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.106, whole genome shotgun sequence.
ACCESSION   DATXQK010000106 DATXQK010000000
VERSION     DATXQK010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.106"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            233..>1730
                     /locus_tag="VC394_001752"
     CDS             233..>1730
                     /locus_tag="VC394_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HEP5995189.1"
                     /translation="MIELNDEFIRKETIELANDGPRVHTTQYETKVPRLHKCYLLFFS
                     IIISSLTIAVPFLTDAANGLQSQNLYIGMMLTKGQLPYSDAFTTGGLFYFVIIALSYY
                     LGSTLWLVFVQVFCFYLSGLYLYKLINYMTCFQKVALTFSISYYLLSVSLGFGGLYPT
                     QLAMPFILISAWFLTKYFACLVKDEAFILFGFVGALAMLIDPSTLIFWSFACVTVFSY
                     NISQKHLARGFYQLLASIFGMILVFYTAGYFILNLQVLNPYLSQTMIYPFTFFKSGNL
                     SLLFGLAIQLFFALGLGLLTGMGNVIRRFKNNSDRVVKWLFVMVILESILVAIFSQDY
                     RPYHLLPLLPFGLILTAIPVGYQYGIGLGQSSHRRRHGKNGVGRVMMIYLKRHFYLPI
                     LIVGTILICSTYCFISSIPLNQERDHIASYLEQKLNKTQSIYVWDDTSKIYLDSKAKS
                     VSQFSSPDINTQKASHRKILEDELLENKAAYIVVNRYKNLPKIIQKVLS"
BASE COUNT          500 a          270 c          298 g          662 t
ORIGIN      
        1 attgactaaa taaaagattc tgttatactt gttttataat attctttgac gataatatca
       61 tttttcaact tactttgttt atgcactgtc actttgctat gtgcaataca aagtcatgtt
      121 atttatctca tactaaaaaa ataatggata atagctatgt tagactagtg atatgaagaa
      181 atattgcttt tattgaatcg ttagagaata tgatatttca aggagaaaaa tagtgatcga
      241 gttaaacgat gaattcatcc gaaaagagac gatagaactg gccaacgatg gcccacgagt
      301 acacaccact caatatgaaa ctaaagtacc tcggcttcat aaatgctatc tgcttttctt
      361 tagcattatt atcagttctt taacaattgc agtacctttt ttaacagatg cagccaatgg
      421 tcttcagtcc caaaacttat atataggaat gatgttaacg aaggggcaac ttccctatag
      481 tgatgctttt acaacaggag ggctctttta ctttgttatc attgcattaa gttattattt
      541 gggatcgaca ctttggctag tctttgttca ggtgttttgt ttttacttat ctggtttata
      601 tctttataaa ctcattaatt atatgacatg ctttcaaaaa gtggctttaa ctttttcaat
      661 tagctactat ttgttgtctg ttagtcttgg ttttggagga ttgtatccta ctcaactggc
      721 tatgccattt atattaatat cggcttggtt tttaactaag tattttgcct gtttagtgaa
      781 agatgaggca tttattcttt ttggctttgt aggtgctctt gcaatgctaa ttgacccgag
      841 tacccttatc ttttggtctt ttgcttgtgt gacagttttt tcttataata taagccaaaa
      901 gcatcttgca agaggttttt atcaactgct agcttcgatt tttgggatga ttttagtttt
      961 ttacacagca ggatatttca ttttgaactt acaagtgcta aatccttatt tatcacaaac
     1021 gatgatttat ccttttactt tttttaaatc aggaaactta tcgttgcttt ttggactggc
     1081 tattcagttg ttcttcgctt tggggcttgg tcttttgaca ggaatgggga atgtcattag
     1141 gcgatttaaa aacaattctg atagggtcgt caagtggcta tttgtcatgg tcattctaga
     1201 atctatactt gtggctatat tttcacaaga ctatcgcccc tatcatcttt tacctctttt
     1261 accttttgga ttaattttga ctgctattcc tgttggctat cagtatggta taggattagg
     1321 tcagagtagt catcgcagac gtcatggtaa aaatggtgtt ggtcgagtaa tgatgattta
     1381 tcttaagaga cacttttatt tgccaatttt aattgtaggg acaatactaa tctgttctac
     1441 ttattgtttc attagtagta ttcctcttaa tcaggagcgt gatcatattg ctagttattt
     1501 agaacagaaa ctaaataaaa ctcaatctat ttatgtttgg gatgatactt ctaaaattta
     1561 tttggacagt aaagctaaat ctgtttctca atttagttct cctgacatca atacgcaaaa
     1621 agcgagtcat cgaaaaatat tagaagatga actattagaa aataaggctg cttatatcgt
     1681 tgttaatcgc tataaaaacc tgcctaaaat cattcaaaaa gtattatcta
//
LOCUS       DATXQK010000107         1715 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.107, whole genome shotgun sequence.
ACCESSION   DATXQK010000107 DATXQK010000000
VERSION     DATXQK010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.107"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            123..1043
                     /locus_tag="VC394_001753"
     CDS             123..1043
                     /locus_tag="VC394_001753"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEP5995190.1"
                     /translation="MSNEFINFEKISRESWKTLHQKAKALLTQEELKSITSLNDNISI
                     NDVIDIYLPLINLIQVYKIAQENLSFSKSLFLKKDIQLRPFIIGISGSVAVGKSTTSR
                     LLQLLLSRTHPNSQVELVTTDGFLYPNQFLIEQGLLNRKGFPESYNMELLLDFLDSIK
                     NGQTAFAPVYSHDIYDIIPNQKQSFNNPDFLIVEGINVFQNQQNNRLYMSDYFDFSIY
                     IDADSSHIETWYIERFLSILKLAKRDPHNYYAQYAQLPRSEAIAFARNVWKTVNLENL
                     EKFIEPTRNRAELILHKSADHKIDEIYLKK"
     gene            1097..1345
                     /locus_tag="VC394_001754"
     CDS             1097..1345
                     /locus_tag="VC394_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEP5995191.1"
                     /translation="MEVKTLANIKSAIKRAELNVKANEKNSAQKSAMRTAIKAFEANP
                     SEELFRAASSSIDKAESKGLIHKNKASRDKARLAAKLG"
     gene            complement(1470..>1715)
                     /locus_tag="VC394_001755"
     CDS             complement(1470..>1715)
                     /locus_tag="VC394_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEP5995192.1"
                     /translation="IIVKTTDKNLLISVIDNGPGITDEEKKKIFDRFYRVDKARTRQT
                     GGFGLGLALAQQIVMSLKGNITVKDNDPKGSIFEVKL"
BASE COUNT          599 a          312 c          246 g          558 t
ORIGIN      
        1 ctaggaatgt gtggcttttt tgagttaatc gtattaattt tcgtgtaaat actaacgaaa
       61 aattatttat tttttaacta aaatggtata atgtaagcac tgtcaaatga ttaggagcta
      121 ccatgtcaaa tgaatttatc aactttgaaa aaatttctag agagtcttgg aaaacattac
      181 atcaaaaagc aaaagctctt ctcacacaag aggaacttaa aagtattact agcttaaatg
      241 ataacatcag tattaacgat gtcattgata tctaccttcc attaattaat ctgatccagg
      301 tttacaaaat tgcgcaagaa aatctttctt tttctaaaag cctttttcta aaaaaagaca
      361 ttcaattaag gccttttatt attggaattt ctggttcagt agctgttggt aaatcaacaa
      421 ctagtcgtct tttacaactt ttattatcac gaacgcaccc caatagtcaa gtcgaactgg
      481 taacgactga tggtttttta tatcctaatc agtttcttat tgaacaagga ctgttaaatc
      541 gtaaaggatt tccagaatct tataacatgg aacttctctt agattttcta gattccatca
      601 aaaatggtca aacggctttt gcacccgtct attctcatga tatttacgat attatcccaa
      661 atcaaaaaca atcatttaat aaccctgact tccttatcgt cgaaggtatt aatgtttttc
      721 aaaatcaaca aaacaatcgt ttatatatga gtgattactt tgatttttct atttatattg
      781 atgcagacag cagtcacatc gaaacctggt acattgaacg gtttctaagc attttaaaat
      841 tagcaaaacg tgaccctcat aattattatg cccaatatgc acaattacct cgatctgagg
      901 ctatagcatt tgcgcgcaac gtttggaaaa ccgttaattt agaaaatctt gaaaaattca
      961 ttgaacctac acgtaaccgt gctgaattaa ttttacataa aagtgctgat cataaaattg
     1021 atgaaattta cctcaaaaag tgataaaggg ttgccaaacg catatatttt ctatataata
     1081 atgtagttag attaatttgg aggtgaaaac attggcaaat attaaatcag ctattaaacg
     1141 cgctgaactt aacgttaaag caaacgaaaa aaactcagca caaaaatcag ctatgcgtac
     1201 tgctatcaaa gcatttgaag ctaacccatc tgaagagctt ttccgcgctg cttcttcaag
     1261 catcgataaa gctgaatcaa aaggtttgat tcacaaaaac aaagcaagcc gcgataaagc
     1321 acgccttgct gcaaaacttg gctaataaat atccaatgaa aactccagct ggagtttttt
     1381 tatacggtaa aaactcaaaa aatacaataa agcgttgaca gttaaaaata actttaaaag
     1441 ggctgacctt attggtcgcc ctttttagct tataatttga cttcaaaaat actaccttta
     1501 ggatcattat cctttactgt aatatttcct tttaaagaca tcacgatttg ctgagctaaa
     1561 gccaacccca agccaaatcc acctgtttgc cgtgttctag ctttgtcaac tcgataaaaa
     1621 cgatcaaaaa tctttttctt ttcttcatct gttatccctg gaccattatc aataacagaa
     1681 attaataaat ttttgtccgt tgttttcact ataat
//
LOCUS       DATXQK010000108         1674 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.108, whole genome shotgun sequence.
ACCESSION   DATXQK010000108 DATXQK010000000
VERSION     DATXQK010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.108"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            172..453
                     /locus_tag="VC394_001756"
     CDS             172..453
                     /locus_tag="VC394_001756"
                     /inference="COORDINATES: protein motif:HMM:NF013753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEP5995193.1"
                     /translation="MTVLNYVNAGDAKSIHNHLFHYTRQVRRHVKVITIGILSAHYYE
                     ITMLFLQAKIILDCFHIPQRLRQAITRFRIRIINTSAIKPIAYRTLKLY"
     gene            complement(930..1017)
                     /locus_tag="VC394_001757"
     tRNA            complement(930..1017)
                     /locus_tag="VC394_001757"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(981..983),aa:Ser,seq:gct)
     gene            complement(1027..1100)
                     /locus_tag="VC394_001758"
     tRNA            complement(1027..1100)
                     /locus_tag="VC394_001758"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1064..1066),aa:Ile,seq:gat)
     gene            complement(1135..1205)
                     /locus_tag="VC394_001759"
     tRNA            complement(1135..1205)
                     /locus_tag="VC394_001759"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1171..1173),aa:Gly,seq:tcc)
     gene            complement(1218..1290)
                     /locus_tag="VC394_001760"
     tRNA            complement(1218..1290)
                     /locus_tag="VC394_001760"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1255..1257),aa:Phe,seq:gaa)
     gene            complement(1311..1384)
                     /locus_tag="VC394_001761"
     tRNA            complement(1311..1384)
                     /locus_tag="VC394_001761"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1348..1350),aa:Met,seq:cat)
     gene            complement(1394..1483)
                     /locus_tag="VC394_001762"
     tRNA            complement(1394..1483)
                     /locus_tag="VC394_001762"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1445..1447),aa:Ser,seq:tga)
     gene            complement(1506..1579)
                     /locus_tag="VC394_001763"
     tRNA            complement(1506..1579)
                     /locus_tag="VC394_001763"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1543..1545),aa:Ile,seq:cat)
     gene            complement(1600..1673)
                     /locus_tag="VC394_001764"
     tRNA            complement(1600..1673)
                     /locus_tag="VC394_001764"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1637..1639),aa:Met,seq:cat)
BASE COUNT          530 a          398 c          271 g          475 t
ORIGIN      
        1 agtaatacaa aattgtttat cttcactcag acaccttatc cttcttagaa tgaattttta
       61 gttacttatc cacaggttaa taacttcttt tcgcctaaaa tattatccac aatagtgaca
      121 ttttctatta tcagttatag tataatttca ttaggaatct aaaaccttag catcacggtt
      181 ttaaattatg taaatgctgg ggacgcaaaa agcattcata atcatctctt tcattatacc
      241 agacaggtga gaaggcatgt caaagttatt accataggca tactcagtgc ccactactat
      301 gagattacta tgctttttct acaggctaaa attatcctcg attgttttca tatcccccaa
      361 cgtcttagac aagctataac tcgctttcgt atccgaatca taaatacttc tgctataaaa
      421 cctatcgcgt atagaactct caaactttac tgaaaactca tacagcatga ttcacggaag
      481 ctcatcaata atagctttat tgcccaattt ttcgtacgcc cataactaac atctagaaaa
      541 gaaaagctct tactcacaag atctctgaat ttcaataatt attcctaact ctatcaactc
      601 ttgctcttcc catttttcag acaattttta aaatttttct aaaagaaaag aaaacaagat
      661 tagcaatata cttaaactca actactctaa ggccaaatta gaaacagtca acaaccttat
      721 caaagccatc aagttaaacg ttttactcct ctacagtagt caaagagcct atcattttag
      781 ttatcgaaat aatgaacgat ttaaatattc tatttagttg gttttgaaga ttacaaaaaa
      841 aagacgataa ccgtcttctt tcccccttat ttataattca tcctttaata tgtttcattt
      901 aacatattaa ccaaaatatc tttacaaaac ggagaataag ggattcgaac ccttgcgcca
      961 gttacccgac ctaacgattt agcaaaccgt cctcttcagc ctcttgagta attctccaaa
     1021 tatatatggg cacgagtgga ctcgaaccac cgacctcacg cttatcaggc gtgcgctcta
     1081 accacctgag ctacgcgccc aagcttttat tgatatagct tgggaaaact ataaagcggg
     1141 tgacgagaat cgaactcgcg acaacagctt ggaaggctgt agttttacca ctaaactaca
     1201 cccgcaatat gataacatgg cgcgagacgg aatcgaaccg ccgacacatg gagcttcaat
     1261 ccattgctct accaactgag ctaccgagcc aacaactaca tacttatgta ttgcgggagc
     1321 aggatttgaa cctacggcct tcgggttatg agcccgacga gctacctaac tgctccatcc
     1381 cgcgatagtt aaaaggagga tgtgggattc gaacccacgc acgcttttac acgcctgacg
     1441 gttttcaaga ccgttccctt cagccggact tgggtaatcc tccaaaataa tgattatcta
     1501 tgatatggac cttgtaggac tcgaacctac gaccgctcgg ttatgagccg agtgctctaa
     1561 ccagttgagc taaaggtccc aagtcatcca taaaaataat agcggcgaag gggatcgaac
     1621 ccccgacctc ccgggtatga accggacgct ctagccagct gagctacacc gcca
//
LOCUS       DATXQK010000109         1658 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.109, whole genome shotgun sequence.
ACCESSION   DATXQK010000109 DATXQK010000000
VERSION     DATXQK010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.109"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(61..1542)
                     /gene="guaB"
                     /locus_tag="VC394_001765"
     CDS             complement(61..1542)
                     /gene="guaB"
                     /locus_tag="VC394_001765"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEP5995194.1"
                     /translation="MSNWDTKFLKKGYTFDDVLLIPAESHVLPNEVDLKTKLADNLTL
                     NIPIITAAMDTVTGSKMAIAIARAGGLGVIHKNMSITEQAEEVRKVKRSENGVIIDPF
                     FLTPEHKVSEAEELMQRYRISGVPIVETLANRKLVGIITNRDMRFISDYNAPISEHMT
                     SEHLVTAAVGTDLETAERILHEHRIEKLPLVDNSGRLSGLITIKDIEKVIEFPHAAKD
                     EFGRLLVAAAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPNR
                     TLIAGNIATAEGARALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIYQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAASDIVFQMLGGIRSGMGY
                     VGAGDIQELHENAQFVEMSGAGLIESHPHDVQITNEAPNYSVH"
BASE COUNT          522 a          381 c          289 g          466 t
ORIGIN      
        1 tttaatataa aaaaagagct ggcacaatat gtcagccctt aaagttacta aattaaatta
       61 tcagtgcaca gaatagtttg gtgcttcatt tgtgatttgg acatcatgcg gatgactttc
      121 aatcaagcct gcacctgaca tttcaacaaa ttgagcattt tcatgtaatt cttgaatgtc
      181 acctgcgcca acatagccca tacctgagcg gataccacct aacatttgga agacaatatc
      241 tgaagcagct cctttataag caacacggcc ttcaatacct tctggaacta gtttattagc
      301 ttcatttacg gaaccttgga agtaacgatc gcttgatcct ttcttcatag ctgcaatgga
      361 acccattcca cgataagttt tgaacttacg gccttggtag atttcagttt cacctggagc
      421 ttcatcagta cctgcaaaca ttgatccaag cataacagca ttaccgccgg ctgcaagagc
      481 cttaacaata tcgccagaat acttgatacc accatcagca ataatcgttt tgccatattc
      541 acgagcaaca gcagcagcat cgtaaatagc agtcacttga ggaacaccga cacctgcaac
      601 cacacgagtg gtacagatag atccgggacc aatacccact ttaacaacat caacacctgc
      661 atcataaaga gcacgcgcac cttcagcagt ggcaatattt ccagcaatca aagtacgatt
      721 tgggaaatga gcacgaatct cagcaatttt acgaagaaca ccagccgaat gaccatgagc
      781 tgtgtcaata acaatcgcat ctgcaccagc ttcaaacaag gcctcggcac gctcaaaagt
      841 atctgaggta acacctaccg cagcagcgac cagaagacga ccaaattcat ctttagcagc
      901 atgtgggaat tcgattactt tttcaatatc tttgatagtg attaaaccag acagacgacc
      961 gctattatca accaaaggaa gtttttcaat acggtgttca tgaagaatac gttctgcagt
     1021 ttcaagatct gtaccaacag cagccgtaac gaggtgctca ctagtcatat gttcagagat
     1081 tggtgcattg taatcagaaa taaaacgcat atcacgatta gtaatgatac cgaccaactt
     1141 acggtttgct aaagtttcaa caattggcac accactgata cggtagcgct gcattaattc
     1201 ttcagcttct gaaaccttgt gttcaggtgt taagaagaat ggatcaatga tgacaccatt
     1261 ttcagaacgt tttaccttgc gaacttcttc tgcttgctca gtaatagaca tgtttttatg
     1321 aataacacct aaaccacccg cacgcgcaat tgcaatagcc attttactac cagtaaccgt
     1381 atccatagct gctgtaataa taggaatatt taatgtcaaa ttgtctgcga gttttgtctt
     1441 taaatcaact tcatttggaa gaacatgact ctctgccgga ataagcaaga catcgtcaaa
     1501 agtgtagcct tttttcaaaa atttagtgtc ccaatttgac atccagacaa tcctcttttc
     1561 ttttattttt agggaatatt accctggtct attttttatt gttactatca tatcacgcta
     1621 aaatcatttg tcaataaaat attcctacag ctgaagtc
//
LOCUS       DATXQK010000110         1657 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.110, whole genome shotgun sequence.
ACCESSION   DATXQK010000110 DATXQK010000000
VERSION     DATXQK010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.110"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(44..916)
                     /gene="sdaAA"
                     /locus_tag="VC394_001766"
     CDS             complement(44..916)
                     /gene="sdaAA"
                     /locus_tag="VC394_001766"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEP5995195.1"
                     /translation="MFYTIEELVKQADQQFNGNIAELMIATEVEMSGRNREDIIKIMS
                     RNLQVMKAAVTEGLTSTKSISGLTGGDAVKMDNYIKKGNSLSDTTILNAVRNAIAVNE
                     LNAKMGLVCATPTAGSAGCLPAVLATAIEKLDLSEKEQLEFLFTAGAFGLVIGNNASI
                     SGAEGGCQAEVGSAAAMSAAALVKAAGGTSHQASQAIAFVIKNLLGLVCDPVAGLVEV
                     PCVKRNALGASFALVAADMALADIDSQIPVDEVIDAMYQVGSAMPTAFRETAEGGLAA
                     TPTGRRYSVEIFGE"
     gene            complement(928..1599)
                     /gene="sdaAB"
                     /locus_tag="VC394_001767"
     CDS             complement(928..1599)
                     /gene="sdaAB"
                     /locus_tag="VC394_001767"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEP5995196.1"
                     /translation="MNTQKFQSVFDIIGPVMIGPSSSHTAGAVRIGKVVHSIFGDIPD
                     EVTFHLYNSFAKTYRGHGTDKALVAGIMGMDTDNPDIKNSLEIAHQKGIKIYWDILKD
                     SNAPHPNTVKISVKKADKTLSVTGISIGGGNIQVTELNGFSVSLSMNTPTIVTVHKDI
                     PGMIAKVTDILSSNNINIATMNVTRESAGEKATMIIEVDSRECQEAANQIAKIPHIYN
                     VNFFD"
BASE COUNT          492 a          335 c          293 g          537 t
ORIGIN      
        1 aaaacgcttt caaatatttc taagaatttt tctaactatc tatttattcg ccaaatattt
       61 ctacgctgta acgtcgtcca gttggagtag cggctaaacc accttctgct gtttcacgga
      121 aagcagttgg catggcagag ccgacttgat acatagcatc aataacttcg tcaacaggaa
      181 tttgagaatc aatatcagct aaagccatat cagctgcgac aagggcgaaa cttgctccta
      241 gagcattgcg tttgacacaa ggcacttcta ctaggccggc aacaggatca cagacaagtc
      301 ctaataaatt tttgataaca aacgcaatag cttgactagc ttgatgagat gttccaccag
      361 ctgctttaac aagagcagct gcactcatag cagctgcaga accaacttct gcttggcatc
      421 caccttctgc accagaaata gaggcgttat ttccaataac aagaccaaaa gctcctgcag
      481 taaataaaaa ctctaattgt tctttttctg ataagtcaag tttttcaata gctgttgcga
      541 gcactgctgg taagcatcct gcacttcctg cagtgggtgt tgcacagaca agtcccattt
      601 tagcatttaa ttcgtttaca gctatagcat ttctaacagc attaagaata gtagtatcag
      661 aaaggctgtt ccctttttta atgtagttat ccattttgac cgcatcgccc cctgtcaaac
      721 cactgataga tttggttgag gttagtcctt ctgttacagc tgctttcata acttgtaaat
      781 tgcgtgacat gattttgata atatcttctc gatttcttcc agacatctcc acttccgtgg
      841 caatcatgag ttcagcaata tttccattaa attgttggtc agcttgtttg acaagttctt
      901 caatagtata aaacatagct ttcctttcta gtcaaagaag ttgacattat aaatatgagg
      961 aatttttgca atttggttgg ctgcttcttg gcactctcta gaatcgactt ctataatcat
     1021 agtagctttt tcaccagcgg attcacgtgt aacattcatt gtggcgatat taatattatt
     1081 ggaagatagg atatcagtta cctttgcgat cattccggga atatctttgt gtacagtaac
     1141 tatagtaggg gtattcatgg ataacgagac agaaaaacca tttagttctg ttacttgaat
     1201 gtttcctcct ccaattgaaa taccagtaac actaagtgtt ttgtcagctt ttttgacact
     1261 gattttaaca gtattgggat ggggagcatt actatctttt aagatatccc aatagatttt
     1321 tatccctttt tgatgagcaa tttctagaga atttttaata tcaggattat cagtatccat
     1381 acccataatt cctgcaacaa gagccttatc agtaccatgt cctctgtagg ttttagcaaa
     1441 tgaattataa aggtgaaacg tcacttcatc aggaatatct ccaaagatgg aatggactac
     1501 cttaccaatt ctgacggcac cagctgtgtg actacttgat ggaccaatca ttactggccc
     1561 aatgatgtca aaaactgatt gaaatttttg tgtattcata ggcaactccg ttttttattt
     1621 ctatgtttat tataacaaaa aaatatcaaa aaacagc
//
LOCUS       DATXQK010000111         1656 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.111, whole genome shotgun sequence.
ACCESSION   DATXQK010000111 DATXQK010000000
VERSION     DATXQK010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.111"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..179)
                     /locus_tag="VC394_001768"
     CDS             complement(<1..179)
                     /locus_tag="VC394_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5995197.1"
                     /translation="MKKKLVGFIVLALSTIILVACSNDSLEGEYYWINDARNQHMATI
                     KGDKGYVESEGGYSI"
     gene            complement(182..520)
                     /locus_tag="VC394_001769"
     CDS             complement(182..520)
                     /locus_tag="VC394_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5995198.1"
                     /translation="MSDIEVYSVLVSRIEYSDGTGSKVRPAVVVKFNDEVIKTLRLTT
                     KYENKSDNIKSQYLEVIDWAKANLKRRSWIDTIQYYDLEDKGFNIKIIGKLSDRDIER
                     LKDFLRAKEV"
     gene            complement(517..810)
                     /locus_tag="VC394_001770"
     CDS             complement(517..810)
                     /locus_tag="VC394_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEP5995199.1"
                     /translation="MSTVAVRVDDQLKDDATELFQSLGLDMSTAVKMFLIQSVKTQSI
                     PFEIKNQSSVSDEEFQNLVETKLKGIRVKASDPESVNAFFGDEDFSEYEEYFK"
     gene            867..1016
                     /locus_tag="VC394_001771"
                     /pseudo
     CDS             867..1016
                     /locus_tag="VC394_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(1522..>1656)
                     /locus_tag="VC394_001772"
     CDS             complement(1522..>1656)
                     /locus_tag="VC394_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_115075552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEP5995200.1"
                     /translation="NQQVKGKGNKLPATGENATPFFNVAALTIISSVGLLSVSKKKED
                     "
BASE COUNT          579 a          310 c          194 g          573 t
ORIGIN      
        1 tttatggaat aaccgccttc tgattcaaca taacccttat cgcctttaat tgtagccata
       61 tgctgatttc tagcatcatt aatccaataa tattcacctt caagtgaatc attagaacat
      121 gctaccaaaa ttattgtaga taaagctaga acaataaaac caactaattt ctttttcata
      181 actaaacttc ctttgctctg agaaaatctt ttaatctttc aatatctcta tcacttaact
      241 taccaataat tttaatattg aatcccttgt cctctaaatc atagtactga attgtatcaa
      301 tccatgaacg cctttttaaa tttgcttttg cccaatcaat aacttctaaa tactgacttt
      361 ttatattgtc agacttattt tcatatttag tagttaatct taaagttttt attacttcat
      421 cattaaattt gactactaca gcaggacgaa cctttgaacc tgtaccatca gaatattcaa
      481 tacgtgaaac taatacagaa tatacctcaa tatcactcat ttaaaatatt cctcatattc
      541 tgaaaaatct tcatctccaa aaaacgcatt tacactttct ggatcactag ccttaactcg
      601 tattcctttt aattttgttt ctaccaaatt ttgaaattct tcatcagaaa cagatgattg
      661 attttttatt tcaaaaggaa tactttgagt tttaacagac tgaatcaaaa acatttttac
      721 tgctgtactc atatccaacc ctaaggattg aaaaagttct gtagcatcat cttttaattg
      781 atcatcaact cttactgcta ctgttgacat aatatcacct caaatcattt ttgtataacc
      841 attgtatcac taactgcaat acaacgcaaa caaaaaagaa aatcgtattt cccagtgaag
      901 agaccctaga acactgtcta gtcacactat ttaatgatta caacttcaag caagaacaac
      961 gagtccacaa aggttttggt cattgtacag acacacttga aagcctattt gattaacaat
     1021 cccagctttg ccagtgtgtt tacacaacat tattgacaca ctccttttat ttttataaat
     1081 tccccttaaa tacatttaat tacttgaaaa gacaaacagt cattttagaa aattttgcat
     1141 ctaaccttac atttgattac acctaaaaat tttaaatcaa caaaagagag cctaggatta
     1201 acatcttagg ctctaatgat acacttcatt tacaaatttc aactaaatag gatcatttct
     1261 tcagttttat agtaagcgtc caataacaag gtatattgga tgcttacgga atagggaaga
     1321 aaatggaaga tacctcgtta ttggacgctt acacaaaatc aaaagcctat cgaaattaat
     1381 aatctcgata ggctttttca agaattaaac tcgaaaaatt gaaatacttt aattaagttt
     1441 gttggagaga gatacataac tttattaatg tgttcacacc aataaatggt gaaaagttta
     1501 gtgacatttt aggtcaaaag attaatcctc ttttttctta gaaacagata ataaaccaac
     1561 tgatgatata attgtcaaag ctgcaacatt aaagaatgga gttgcattct cacctgttgc
     1621 tggtagttta tttcctttac ctttgacttg ctgatt
//
LOCUS       DATXQK010000112         1605 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.112, whole genome shotgun sequence.
ACCESSION   DATXQK010000112 DATXQK010000000
VERSION     DATXQK010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.112"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..>1605
                     /locus_tag="VC394_001773"
     CDS             <1..>1605
                     /locus_tag="VC394_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP5995201.1"
                     /translation="LAILGFSKKELTQLLTLENLVILVLSYLVSLLLGPTLYFLAVLV
                     ITHLLELTLEVQWFITANEIIESLGILVVVFLINVITNGLIISKQSLIEFVNFSRKAE
                     KKIKIRKVRAIIAITALLLSYILCLATVFSSTRNMLLSIGMVPVSLLIIVLVVLGTVF
                     TIRYGLAFIVSLLKGNKKRLYRPLSNIIYPKFNYRIATKNKLLTVLGGLLTVTVSVAG
                     MMVMLYAYSLNGIERLTPSAIEYNVKSENGQVNVTTILENDQVSLVDVDLLRLNTNPE
                     VTITESGQTIPYFDIINYSDYKELMKAQGRINSIEGSKSLPLLINYYPTEISLGKTFN
                     LGNAYDVTVKQVSTHNVFSFSTSVTTLVVSDKLYAKLSSCFPEKEMTIRTFNGNSIRS
                     SEAFYNQFSMVPDVISSYSKEYTVKTANIATYIFITFLSILFIICTGSILYFTSLIEI
                     MENKEEYGYLSKLGYSKKMIHRIPRYETGILFLIPVFIGIVNGGMLLIYYKYLFMDTL
                     VAGNIIMLSLLLCLLFFLIIYGTFYVL"
BASE COUNT          525 a          223 c          278 g          579 t
ORIGIN      
        1 ttagcaatac tagggttttc aaaaaaagaa ttaacacaat tactgacttt agaaaatctt
       61 gttattctag ttttaagtta cttggtaagt ttactattgg gaccgacctt atatttttta
      121 gcggtactgg taattactca tctattggaa ttaacattgg aagttcagtg gtttattaca
      181 gctaatgaga ttatagagtc tttaggaata ttagtcgtag tttttctgat taatgtcatc
      241 acaaatggac ttatcattag taaacagtct ttgattgaat ttgttaattt ctcaagaaag
      301 gctgagaaaa aaattaagat aagaaaagtc agagctatta ttgctatcac tgcattgcta
      361 ttgtcatata ttttatgttt ggcgacagtg ttttcatcca cacgaaatat gctattaagc
      421 atagggatgg taccggtttc tctattgata attgtcttag ttgttttagg aacagtgttc
      481 accatcagat atggattggc ttttatagtt tcgttattaa aaggaaataa aaaaaggtta
      541 taccgtcctc tgtctaatat catctatccc aaatttaact atcgtattgc aacaaaaaat
      601 aaattattaa cagtcttggg aggtctttta acagtaaccg tttcagttgc tggaatgatg
      661 gtaatgctct atgcttattc tcttaatggg atagagaggt tgactccatc tgccatagaa
      721 tataatgtta aatcagaaaa cggtcaagtc aatgttacaa ctattttaga gaacgaccaa
      781 gtgagcttgg ttgatgttga cctattgaga ttgaacacta acccagaagt aactatcaca
      841 gaatctgggc aaacaattcc ttattttgat ataattaatt acagtgatta taaagagtta
      901 atgaaagctc aagggagaat aaattctatt gaaggtagta agtcactccc attgttaata
      961 aattattatc caacagaaat tagccttgga aaaaccttta acttaggaaa tgcatatgat
     1021 gttactgtaa aacaagtatc aacgcataat gtttttagtt tttctacaag tgtcacgacc
     1081 ttggttgttt ctgataaatt atatgctaaa cttagttctt gttttccaga gaaagaaatg
     1141 acaattagga cttttaatgg aaattcgatt aggtcaagtg aagcatttta caatcagttt
     1201 agtatggttc ctgatgttat cagtagttat agtaaggaat acacagtaaa gactgctaat
     1261 attgcgactt atatctttat aactttccta tccatactct ttattatttg tacaggtagt
     1321 attctgtact ttacaagcct catcgaaatc atggaaaata aagaagaata tggctatcta
     1381 agtaagctag gttatagtaa aaaaatgatt catcggattc ctcgatatga aacaggtata
     1441 cttttcctta ttcctgtatt cattgggatt gtaaatggtg gtatgttgct tatttactat
     1501 aaatatttat tcatggatac attggtagca ggcaatatca taatgttatc tttattgctt
     1561 tgtctgcttt tcttcttgat aatatatggc acattttatg tattg
//
LOCUS       DATXQK010000113         1595 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.113, whole genome shotgun sequence.
ACCESSION   DATXQK010000113 DATXQK010000000
VERSION     DATXQK010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.113"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..587
                     /locus_tag="VC394_001774"
     CDS             <1..587
                     /locus_tag="VC394_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEP5995202.1"
                     /translation="TLDQLKQFLELVEKTDNIEKIALFRLLAFTGIRKGELLALTWDD
                     LNGNTLSINKAVTRTQVGLEIDVTKTKSSDRLISLDDETLEILQELHETFPTSTLMFQ
                     SESGGIMTPSLPRKWLLQIIKGTDLPQITIHGFRHTHASLLFESGLSLKQVQHRLGHG
                     DLQTTMNVYTHITQSAIDDIGTKFNQFVTNKQLD"
     gene            950..1570
                     /locus_tag="VC394_001775"
     CDS             950..1570
                     /locus_tag="VC394_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3862 domain-containing protein"
                     /protein_id="HEP5995203.1"
                     /translation="MTKKHLLTLLLISFFTSFLVACSTTKDKEPQPSDSEIITPRLHQ
                     AAHQDKRANFEKIKLATVDSSFTGGTSLEELISLFGEPSQHDPKTAGEVTIDAYTWQF
                     DQVTLTVNLYQNSSIVKTISNFTFARELGLSQKEYQQLQKGMSYEDVKKILTEPDNYS
                     QASSSDHQTLQAIWVSGLKTDTSGANISLVFENNQLTEMSQVGLEE"
BASE COUNT          565 a          310 c          246 g          474 t
ORIGIN      
        1 atacacttga tcaacttaaa caattccttg aactagttga aaaaactgac aacattgaaa
       61 aaatagcctt gtttagatta ttagcattta ctggaatacg aaaaggggag cttctggcac
      121 taacttggga tgatttgaat ggtaatactc tatcaattaa taaagctgtc acacgtactc
      181 aagttggact agaaatagat gttacgaaga caaaatcgag cgatagatta atcagcttag
      241 atgatgaaac tttggaaatt ttacaagaac ttcatgaaac ttttcctact tctactctta
      301 tgttccaatc tgaatcaggt ggaattatga cgccaagttt accacgaaaa tggctattgc
      361 aaattatcaa agggacagac ttaccacaaa tcacaattca tggtttcagg cacactcatg
      421 caagcttact tttcgaatca ggtctatcct tgaaacaggt gcaacatagg ttagggcatg
      481 gagatttaca gacaactatg aacgtatata ctcacatcac gcaatcggca attgatgaca
      541 ttggaactaa attcaatcaa tttgttacta acaagcaact agattgacaa ctaattctca
      601 acaaacgtta atttaacaac atccaagtaa ctcccaccag ctccatcaat gcttaccgta
      661 agtaatcata acttactaaa accttgttac atcaaggttt tttctttttg tcttgttcat
      721 gagtttccgt tatccctata gccataccat cacgcttacc gatactccat caataccact
      781 caaaaccttg tcatatcaga tctttttgac catttttttc atgaatatct aaaaaaaaga
      841 atcaaaatag tactaaattc cccatagcgc atgcgctatg gggaatcata gctatatctt
      901 gtttataatt tatgatataa tacagcaaaa atagtttagg aaataacata tgacaaaaaa
      961 acacttacta acacttcttc tcatctcttt ttttactagc tttttggtag cttgttcaac
     1021 aacgaaagat aaagagcctc aaccgtctga ttcagaaatc attactcccc gactacacca
     1081 agccgctcat caagataaac gcgctaactt tgaaaaaatt aaacttgcga ctgttgattc
     1141 ctcatttaca ggagggacaa gccttgaaga acttatttca ctctttggag agcctagcca
     1201 acatgatcca aaaacagcag gcgaagtaac aatcgacgct tatacttggc agtttgatca
     1261 agttactctc actgttaatc tttatcaaaa tagtagtatt gttaaaacca tctctaattt
     1321 tacctttgca agagagttag gcttatcgca aaaggaatac caacaattac aaaaaggaat
     1381 gtcttatgaa gacgttaaaa agatcttaac agaacctgat aattatagcc aagcgtcatc
     1441 tagtgatcat caaactttgc aagcgatttg ggttagtggc ttaaagacag atacaagcgg
     1501 agctaatatt tctctcgttt ttgaaaataa tcagttaaca gaaatgtctc aggtaggact
     1561 tgaagaataa aaaatgagta atatattttg ctaaa
//
LOCUS       DATXQK010000114         1432 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.114, whole genome shotgun sequence.
ACCESSION   DATXQK010000114 DATXQK010000000
VERSION     DATXQK010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.114"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            254..403
                     /locus_tag="VC394_001776"
     CDS             254..403
                     /locus_tag="VC394_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5995204.1"
                     /translation="MRERNEKFIGVLKDMHMQILRDFTNEEDPLTRCELAKGFLEIGD
                     YLSKQ"
     gene            complement(389..604)
                     /locus_tag="VC394_001777"
     CDS             complement(389..604)
                     /locus_tag="VC394_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008087450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5995205.1"
                     /translation="MKISNSKDLALAIVASSSPTLSIEDKIKLYEDSVEAIKKHNLPF
                     VEAEKQEQINNGKVIAEALERGESLFG"
     gene            663..821
                     /locus_tag="VC394_001778"
     CDS             663..821
                     /locus_tag="VC394_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5995206.1"
                     /translation="MNWKKLMFGDLEYTFASRDGKEKTSIEFEGGLLPALLVLGGITW
                     LAAWFITK"
     gene            complement(851..>1432)
                     /locus_tag="VC394_001779"
     CDS             complement(851..>1432)
                     /locus_tag="VC394_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011284882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5995207.1"
                     /translation="TNFLIPLISAIVSVSSIFISNWLGYRSQIRKLKFDEEKEIYLTL
                     YVPLIKWMNSQSFNNKSYYWLVAFPRYTTNAQDFLTGLLLKNFEKLPVSVAMRYSEYT
                     LNSATSLHFYRNTEYDYDYETFAKKASELFDLIIEQLLTEGTILSQKLSLPNLSKSTL
                     ENFLADKKNYIGPRFLSLETHNKPLRPERPLPF"
BASE COUNT          468 a          219 c          301 g          444 t
ORIGIN      
        1 gagatatact ataatcaagc ttaagggatt aagcaaaaat gaaagtaggt acagctgatg
       61 aagtctaggc taaacaaaaa gcctaaacac aaagaactag aagtcgaaat caagattctt
      121 tggtttaagc ttaagattca ctactcaata gagtggtgag gataattaga gggctaagaa
      181 gccctctccc cctaacgggg taagtttagt ttagcacatt ggctgtatct ccgcaagaat
      241 gaaaggagag gttatgagag aacgaaatga aaagttcatc ggagtgctaa aagatatgca
      301 tatgcaaatt ttgcgagact tcacaaacga agaagacccc ttaacaaggt gcgagcttgc
      361 taaagggttc ctcgaaattg gtgactattt atccaaacaa tgactcgcca cgctcaagag
      421 cttcggctat aactttacca ttattgattt gttcctgctt ttcggcttca acgaaaggta
      481 aattatgttt cttaatagct tccacagagt cttcgtaaag tttgatttta tcttcgatag
      541 acaaagtagg gctagaagaa gcgacaatag cgagagctaa atcttttgaa ttagaaattt
      601 tcattagctt atcctccttt ccgctaggat aagttgatta taacattttt aggaggcaca
      661 aaatgaattg gaaaaaacta atgtttggcg atttagaata cacgtttgcc agtcgtgacg
      721 gcaaagaaaa aacaagcatc gaatttgaag gtggcttatt gccagcgctg ttggtgttag
      781 gtggtatcac ttggttagca gcttggttta ttacaaaata aaaagcccac aaggggcgga
      841 aaggggggag ttaaaatggc agtggtcttt caggtcttag gggtttgtta tgggtttcta
      901 gtgataaaaa tctgggaccg atatagtttt tcttgtccgc caaaaagttc tctaaagtgg
      961 atttggataa gtttggtaag cttagctttt gggataatat tgtcccttct gttagcaatt
     1021 gttcaataat gagatcaaat aattcagatg cttttttggc aaatgtttca taatcataat
     1081 cgtattcggt gttacgataa aaatgtaaag aggttgcaga atttagggtg tattcagagt
     1141 atctcatagc aactgatact ggtaattttt caaaattttt taacaacaag cctgtcagaa
     1201 aatcttgtgc atttgttgta tatctaggga acgccactag ccaataataa cttttgttat
     1261 tgaatgattg cgaattcatc cattttatta acggtacata gagggttagg tatatttctt
     1321 tttcttcatc aaattttaat ttcctgattt gacttctata accaagccaa ttgcttataa
     1381 aaatagaaga aacagagact attgctgaaa ttaacggaat taaaaaattg gt
//
LOCUS       DATXQK010000115         1429 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.115, whole genome shotgun sequence.
ACCESSION   DATXQK010000115 DATXQK010000000
VERSION     DATXQK010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1429
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.115"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            264..380
                     /locus_tag="VC394_001780"
                     /pseudo
     CDS             264..380
                     /locus_tag="VC394_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611995.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(566..>1429)
                     /locus_tag="VC394_001781"
     CDS             complement(566..>1429)
                     /locus_tag="VC394_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HEP5995208.1"
                     /translation="ADLEREAAKVREAIANDPTLTKADKAKQTEAVAKALKAAIAAVD
                     KATTAEGINQELGKGITAINKAYRPGEGVEAHKEAAKANLEKVAKETKALISGDRYLS
                     ETEKAVQKQAVEQALAKALGQVEAAKTVEAVKLAENLGTVAIRSAYVAGLAKDTDQAT
                     AALNEAKQAAIEALKQAAAETLAKITTDAKLTEAQKAEQSENVSLALKTAIATVRSAQ
                     SIASVKEAIDKGITAIRAAYVPNKAVAKSSSANHLPKSGDANSIVLVGLGVMSLLLGM
                     VLYSKKKESKD"
BASE COUNT          408 a          296 c          256 g          469 t
ORIGIN      
        1 atcaagtaga aatgtcttta ttaagcgaat caatcaaggt tgtagagtaa atactggact
       61 agatttcatg tagaaattag taaaagttag gagatttttt ggtcgtttca aacgataaca
      121 tcatcttctt ataacaagaa cttcactcag agataaacta ttctaatact ttagtagcct
      181 cttttgagct tgtagcacga aaattcgctc gaactgattt aaaataggca ttttatctgc
      241 tcgcttgatc cctactgcgc gaccaagaaa ccttttattc actagaacaa ttaaccctta
      301 aaaccaagga ggaagtccac tggtggaact accatcacct tcacagtaat cttaattatc
      361 aaacacccat aactaaatga gtcatcgttt gaacaaaaaa cgccttataa attctgtaca
      421 gaaaactgtt gccttttcaa ttgaactttg aaatgttttc aatacactcc gttcttgggc
      481 ccttacgatc caaaaaacca aggtgatttc aaaatcacct tggtttgaaa atatagtaga
      541 aagcaatttg gataaagcca ggttcttagt ctttactttc ttttttcttg ctataaagca
      601 ccatacctaa aagaagagac ataactccta agccaacaag aacaattgag tttgcatcac
      661 ctgattttgg aagatggttc gctgacgatg attttgcgac tgccttatta ggcacatagg
      721 ctgcacggat agcagtaata cctttatcta ttgcttcttt cacagacgca atagattgtg
      781 ctgaacgaac agtcgcaata gccgtcttaa gcgctaatga tacattttct gattgttcag
      841 ctttttgagc ttcagtcaat ttagcatcag ttgtaatctt agcaagtgtt tctgccgcag
      901 cttgtttaag agcttcaata gcagcttgtt tcgcttcgtt aagagcagct gttgcttgat
      961 cagtatcttt agctaaacca gcaacatatg ctgaacggat agctacagta ccaaggtttt
     1021 ctgccaactt aacagcttca actgtcttag cagcctcaac ttgaccaagt gctttcgcaa
     1081 gagcttgctc aacagcttgt ttttggactg ctttttcagt ttcgctcaag taacggtctc
     1141 ctgaaataag agctttagtt tctttagcta ctttttcaag attagcttta gcagcttctt
     1201 tatgtgcttc aacaccttca cctggacggt aagctttgtt gatagctgtg atgcctttac
     1261 caagttcttg gttaatgcct tcagctgttg tcgctttatc aaccgctgcg atagcagctt
     1321 taagagcttt agcaacagct tctgtttgtt tagctttatc agcttttgtt aaggttgggt
     1381 cgttagcgat agcttcacga accttagcag cttctctttc aagatcagc
//
LOCUS       DATXQK010000116         1399 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.116, whole genome shotgun sequence.
ACCESSION   DATXQK010000116 DATXQK010000000
VERSION     DATXQK010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1399
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.116"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            78..812
                     /locus_tag="VC394_001782"
     CDS             78..812
                     /locus_tag="VC394_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HEP5995209.1"
                     /translation="MNLSIFGLKNIPYLKEGDSIEKLIEESIKTSGFFIEDNDVLCIA
                     SKVVSIAEGQVMSLNEIQVSDVAKEIHRNIPRKDPRIIEIMLNLVNRDLSRLDIKKNY
                     IGCRLENGLKLTSGGIDRKSVDEVFLLPNNPDASAKRISEYLKKSLGKNVAVVITDSD
                     GREDKRGATQVAIGIYGIHPLRKTEVIDSQGETIKFQEETLCDMIAACAGLVMGQRGT
                     GIPAVLIKGLDYKWSENTSIKEALNE"
     gene            805..>1399
                     /locus_tag="VC394_001783"
     CDS             805..>1399
                     /locus_tag="VC394_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HEP5995210.1"
                     /translation="MSRNQDCKNILIIGFGKIGKIKAYKWLSRGYEVYVKDLKFSSMK
                     YLNTNSDVLDDLSRQYFTIEICTPTNHHLTLLKTVITRYVYSYISIEKPLCNRLEDLE
                     EMKSLVESHPHLAKKMFASEQYFFSKILEKLLQVDCFSLDKIKEITVNFSKDRIEDNE
                     NGRFLDKEILGYGIEIPHIIAVISYLGISLEEFKKGIF"
BASE COUNT          518 a          171 c          289 g          421 t
ORIGIN      
        1 aaaagaaata gacaaaaagg cagaagtagt gaggaagtct tatctgacac taaatgagta
       61 tattaatgga ggctaaaatg aatttgagta tatttggttt aaagaatatt ccttatttga
      121 aagaagggga tagtatagaa aagcttattg aagaaagcat taaaacttca ggatttttta
      181 tcgaagacaa tgatgtgctt tgcatagctt ctaaagttgt atctatcgca gaaggccagg
      241 tgatgtcttt aaacgagata caagtgagtg atgtagcaaa agaaatccac aggaatattc
      301 ctcgtaaaga tcctagaata attgaaatta tgctaaattt agtcaataga gatctgtcac
      361 gtttagatat caaaaaaaat tacattggtt gtcgcttgga aaatggttta aagttgacaa
      421 gtggtggaat cgatagaaaa tcagtagatg aagtatttct tttacctaat aatccggatg
      481 cttctgcaaa aagaatttcg gaatatctta aaaaatcact tggaaaaaat gttgcagttg
      541 ttataacaga tagtgatggt agagaggata aaagaggtgc tactcaagta gcaattggta
      601 tttatggtat tcatcctttg agaaaaacag aagtaataga ttcacaagga gagacaataa
      661 aattccaaga agagactttg tgtgatatga ttgcagcttg tgctggctta gtaatggggc
      721 aacgtggaac tggaattcca gctgtcctca ttaagggctt ggattataaa tggtctgaaa
      781 atactagtat taaggaagcg ttaaatgagt agaaatcagg actgtaagaa tattctaatc
      841 attggttttg gaaaaattgg gaaaattaaa gcgtacaagt ggctatcacg cggatatgag
      901 gtatatgtca aagatttgaa attctccagc atgaaatacc ttaatacaaa tagtgatgtg
      961 ttggatgatt tatcacgaca atattttact attgagattt gtactccgac aaatcaccac
     1021 cttacgctat tgaaaaccgt tataacaagg tatgtctatt cttatattag cattgaaaaa
     1081 ccactgtgta acaggttaga agatttagaa gaaatgaagt cgctagtaga aagtcaccca
     1141 catctagcaa aaaaaatgtt tgccagtgaa caatacttct tttcgaaaat tttggaaaaa
     1201 ctgttacaag tagattgttt ctctctagat aaaatcaagg aaataacagt taatttttct
     1261 aaagatagga ttgaggataa cgagaatggg agatttttag ataaagagat attgggctat
     1321 ggaatagaga ttcctcatat cattgctgtc atttcttatt taggaatttc attagaagaa
     1381 ttcaaaaaag ggatctttg
//
LOCUS       DATXQK010000117         1241 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.117, whole genome shotgun sequence.
ACCESSION   DATXQK010000117 DATXQK010000000
VERSION     DATXQK010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.117"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            15..1208
                     /locus_tag="VC394_001784"
     CDS             15..1208
                     /locus_tag="VC394_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEP5995211.1"
                     /translation="MIDSLLLKSLHSLLGLTITVCDQNFSVIREYKSDKTISLFYNHY
                     LILSNFSKTQHDFLFHYGSLGELFLVHHIQQYYIIIGPWRSNAIDPLLLKKKLTETQI
                     NASEQDYFIDRLSQLPFFSLSQIRELLIVTNYCLTGVVKDKLSEPLHYYTKGWSNSFD
                     LDKIKQFSKQNMSSYKYQYHFENNILKAVKSGSEFLLKETVEHFSNSIVPIISGDELR
                     SEKNYSIIIYDRLSQATIQAGLDIETAYRARDRFIKETESTISLNEVLKLRDTAILFY
                     TQQVDSLKRHLETPHSQTIVAVIRYLENNLNRFIKTEEIAKECHMSESKLRKLFKQEK
                     HITIQQYFINLKIEAAKQLLDENKKVEEVSNLLGFSTSSNFSRTFKKIVGISPLEYKQ
                     KLKTI"
BASE COUNT          451 a          211 c          172 g          407 t
ORIGIN      
        1 aggtggtaac tcatatgatt gattctttat tgttgaaatc attacacagt cttctaggtc
       61 taactattac tgtttgtgat caaaattttt ctgttatcag agagtataaa tccgataaaa
      121 ctatttcact tttttacaat cattacctta ttttaagtaa ctttagtaaa actcaacatg
      181 attttttatt tcattatggc tctttaggag aactttttct tgtccaccat attcaacaat
      241 attatattat catcggtccc tggcgtagca atgctattga tcctttactt cttaagaaaa
      301 agctgactga aacacaaatc aatgctagtg aacaagacta ctttattgat agactatccc
      361 aattaccttt cttctcactt agtcaaatcc gagaattact aatagtaacc aattattgtc
      421 taactggggt tgtgaaagat aaattatcag agcccttaca ttactacacg aaagggtgga
      481 gcaactcctt tgatctagat aaaattaagc aattttctaa acagaatatg agttcttaca
      541 aatatcagta tcattttgaa aataatattc taaaagctgt taaatcaggt agtgaatttc
      601 ttttgaaaga aacagtggaa cactttagca attctatcgt acctataatc agtggagatg
      661 aattgcgatc tgaaaaaaac tactccatca ttatttatga tcgtctctca caggctacca
      721 tccaagcagg acttgatatc gaaacagctt atcgagcacg agatcgtttt ataaaagaaa
      781 ccgagtcaac tataagtcta aatgaagttt taaaattacg tgatactgct atcttattct
      841 atactcaaca agttgattct ttaaaaagac atctcgaaac ccctcattct caaactattg
      901 tcgcagtgat tcgatatctt gaaaataatc taaatcgctt tattaaaaca gaagaaatag
      961 ctaaagaatg tcacatgagt gaatcgaagt tacgaaaact atttaagcaa gaaaaacaca
     1021 tcactattca acaatatttt ataaacttaa aaatcgaagc tgctaagcag ttactagacg
     1081 aaaataagaa agtagaagaa gtttccaatt tacttggatt ttccacttct tctaattttt
     1141 caaggacatt taaaaaaata gtgggaatta gcccactaga atataagcaa aagcttaaga
     1201 caatatagta atcgaaaaat attcattttg cttaatattc c
//
LOCUS       DATXQK010000118         1240 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.118, whole genome shotgun sequence.
ACCESSION   DATXQK010000118 DATXQK010000000
VERSION     DATXQK010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.118"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            29..1054
                     /locus_tag="VC394_001785"
     CDS             29..1054
                     /locus_tag="VC394_001785"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922160.1"
                     /note="This family includes IgM or IgG-cleaving cysteine
                     proteases; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IdeS/Mac family cysteine endopeptidase"
                     /protein_id="HEP5995212.1"
                     /translation="MRKRCYSTSAAVLAAVTLFALSVDRGVIADSFSANQEIIYSEVT
                     PYHVTSVWTKGVIPPANFTQGEDVFHAPYVANQGWYDITKTFNGKDDLLCGAATAGNM
                     LHWWFDQNKEKIEAYLKKHPDKQKIMFGDQELLDVRKVINTKGDQTNSELFNYFRDKA
                     FPGLSARRIGVMPDLALDMFINGYYLNVYKTQTTDVNRTYQEKDRRGGIFDAVFTRGD
                     QSKLLTSRHDFKEKNLKEISDLIKKELTEGKALGLSHTCANVRINHVINLWGADFDSN
                     GNLKAIYVTDSDSNASIGMKKYFVGVNSAGKVAISAKEIKEDNIGAQVLGLFTLSTGQ
                     DSWNQTN"
     gene            1095..>1240
                     /locus_tag="VC394_001786"
     CDS             1095..>1240
                     /locus_tag="VC394_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5995213.1"
                     /translation="MLRTNTIAGNFFIDGQQQKTLINPIKIDKDIVTIQEFDFKIRKF
                     LMES"
BASE COUNT          440 a          197 c          239 g          364 t
ORIGIN      
        1 ataaaattat atagaaaaag gtattcttat gagaaaaaga tgctattcaa cttcagctgc
       61 agtattggca gcagtgactt tatttgctct atcggtagat cgtggtgtta tagcagatag
      121 tttttctgct aatcaagaga ttatatattc ggaagtaaca ccttatcacg ttacttccgt
      181 ttggaccaaa ggagttattc ctccagcaaa cttcactcaa ggcgaagatg tttttcacgc
      241 tccttatgtt gctaaccaag gatggtatga tattaccaaa acattcaatg gaaaagacga
      301 tcttctttgc ggggctgcca cagcagggaa tatgcttcac tggtggttcg atcaaaacaa
      361 agaaaaaatt gaagcatatc taaaaaaaca cccagataaa caaaaaatca tgtttggtga
      421 tcaagaatta ttggatgtaa gaaaagttat taatactaaa ggtgaccaaa caaatagcga
      481 gctttttaat tatttccgag ataaagcttt ccccggtttg tcagcacgcc gaattggagt
      541 tatgcctgat cttgctttag atatgtttat caatggttat tacttaaatg tttataagac
      601 acagactact gatgtcaata gaacctatca agagaaagat cgccgaggtg gtatttttga
      661 cgccgtattt acaagaggtg atcaaagtaa gctattgaca agtcgtcatg attttaaaga
      721 aaaaaatctc aaagaaatca gtgatctcat taagaaagag ttaaccgaag gcaaggctct
      781 aggcctatca cacacctgcg ctaacgtacg catcaaccat gttataaacc tgtggggagc
      841 tgactttgat tctaacggga accttaaagc tatttatgta acagactctg atagtaatgc
      901 atctattggt atgaagaaat actttgttgg tgttaactcc gctggaaaag tagctatttc
      961 tgctaaagaa ataaaagaag ataatattgg tgctcaagta ctagggttat ttacactttc
     1021 aacagggcaa gatagttgga atcagaccaa ttaaagcgta tcaataagaa agattacatt
     1081 caacagatga tggtattcta aggactaata ctattgcagg gaattttttt atagatgggc
     1141 aacagcaaaa aacattaata aatcctataa aaatcgataa ggatattgtt acgattcaag
     1201 agttcgattt taaaataaga aaatttttga tggaatcaaa
//
LOCUS       DATXQK010000119         1198 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.119, whole genome shotgun sequence.
ACCESSION   DATXQK010000119 DATXQK010000000
VERSION     DATXQK010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.119"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(43..1164)
                     /gene="mnmA"
                     /locus_tag="VC394_001787"
     CDS             complement(43..1164)
                     /gene="mnmA"
                     /locus_tag="VC394_001787"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEP5995214.1"
                     /translation="MTDNSKIRVVVGMSGGVDSSVTALLLKEQGYDVIGVFMKNWDDT
                     DEFGVCTATEDYKDVAAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYAQVKRDENGTVHMLRGADNGKDQTYFLSQLS
                     QEQLQKTLFPLGHLQKSEVREIAERAGLATAKKKDSTGICFIGEKNFKQFLSQYLPAQ
                     KGRMMTIDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNQPWFVVGKDLSQNILYVGQ
                     GFYHEALMSNSLDASVIHFTREMPEEFTFECTAKFRYRQPDSHVAVHVRGDKAEVVFA
                     EPQRAITPGQAVVFYDGKECLGGGMIDMAYKNGQPCQYI"
BASE COUNT          362 a          278 c          202 g          356 t
ORIGIN      
        1 attgttgaat gtccctttct ttttatctta ataaaagatt cattaaatgt actgacaagg
       61 ctgcccattt ttataagcca tgtcaatcat accaccacca aggcattctt ttccgtcata
      121 gaaaacaaca gcttgaccag gagtaatcgc gcgctgtggc tctgcaaaaa caacttctgc
      181 cttatctcct cgaacatgca ccgccacgtg tgaatcgggt tgacgatagc gaaatttagc
      241 agtacattca aatgtgaact cttctggcat ctcacgtgta aaatggatga ctgaggcgtc
      301 aagactattt gacattaggg cttcatggta gaaaccttga ccaacgtata aaatattttg
      361 cgacaaatct ttaccgacaa caaaccaagg ttgattatcc ccaccatgct gaccaccaat
      421 gcccagacca ccacgctgac caatagtata atacataaga ccggcatgtt ctcccatatc
      481 gcgaccatca atagtcatca tgcgaccttt ttgtgccggt aagtactggc tcaaaaactg
      541 tttgaaattc ttttcaccaa taaaacaaat acctgttgaa tctttctttt tagccgttgc
      601 aagtcctgct cgctcagcga tttctctaac ttctgacttt tgcaaatgac caagggggaa
      661 taaagtcttt tgtagttgtt cctgtgatag ttgacttaaa aagtaggttt ggtctttgcc
      721 attatcagct ccacgtaaca tgtgaaccgt accattttca tcacgcttga cttgagcata
      781 gtgtcccgtt gctacataat ctgcacctaa agtcatggca tagtcaagga aagctttaaa
      841 ttttatttcc ttattacaca tgacatcggg atttggagta cgacctgctc ggtattcggc
      901 caagaaatat tcaaatactc tgtcccaata ttctttttca aaattgactg agtagtaagg
      961 aataccgatt tgatcagcaa cggctgctac atctttgtaa tcttcagtag ctgtgcaaac
     1021 accaaattca tctgtatcgt cccagttttt catgaagaca ccaataacat cataaccttg
     1081 ctcctttaaa agtagagctg ttacagatga atcaacgcca ccactcatac caacgacaac
     1141 acgtatcttt gagttatctg tcataaaaaa ttctcccatc aataataaga tagaaaaa
//
LOCUS       DATXQK010000120         1198 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.120, whole genome shotgun sequence.
ACCESSION   DATXQK010000120 DATXQK010000000
VERSION     DATXQK010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.120"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..>1198)
                     /locus_tag="VC394_001788"
     CDS             complement(<1..>1198)
                     /locus_tag="VC394_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEP5995215.1"
                     /translation="FRKKSGLKQKEFAKCIGISQAMLSQYESGKKKLSLEKFQEMKTH
                     FGYLTDNDSSRLQVTIDYVRITLKSVRDLEFFCRNFLHCRFKEFSSVESKLMNYNHIW
                     KRGDIWIFDYADKHDTGNYQITLQLSGQGCRQLELLMEQENFAWLDWLIYLRRSYRDE
                     MNVTRFDLAIDELYLGKDRENEHFLLSDMIAKYYHHELAFESLRTWNYIGGGALNFND
                     MEEIEQNRQGISLYFGSRQSEMYFNFYEKRYELAKLEGISVEESLEIFEIWNRYEIRL
                     SQSKANAVVDEFISGIPLGEIARGLINSKIQVYDGKNKYGAFMADQKWQMMFGGVEPL
                     TFVTRPESYNIERTIRWLIHQVSDSLAMVSEYDSMIDGDNLQMIINSGEVNERGEKIL
                     ETIRYNL"
BASE COUNT          408 a          246 c          126 g          418 t
ORIGIN      
        1 ctagattata tcgaatagtt tctaatattt tttcacctcg ttcattcact tcaccagaat
       61 taatgatcat ttgtagatta tccccatcaa tcatactgtc atattcacta accatagcta
      121 gagaatcaga tacttgatga atcaaccagc gaatagtacg ttcaatatta taagattctg
      181 gtctcgtgac aaatgttaga ggttcaacac ctccaaacat catctgccac ttttgatcag
      241 ccataaatgc accatactta ttttttccat cataaacttg aattttacta ttaatcaatc
      301 ctctcgcaat ttctcctaat ggaataccag aaataaattc atctaccact gcattcgcct
      361 tagattgaga taatcggatt tcataccgat tccatatttc aaatatttct aaagattctt
      421 caacagagat gccctctaat ttagctaatt catacctttt ctcataaaag ttaaaataca
      481 tttcagactg tcgactacca aaatataacg aaataccttg acgattctgt tcaatttctt
      541 ccatatcatt aaaatttaaa gcaccaccac caatataatt ccatgttcgc aaactttcaa
      601 aagctaactc atgatgataa tatttggcaa tcatatccga taaaagaaag tgttcatttt
      661 ctctatcctt acccagatac aattcatcaa tagctaagtc aaaacgtgta acattcattt
      721 catctcgata agaacgtctt aagtagatta gccaatctaa ccaagcaaaa ttttcttgtt
      781 ccataagtaa ttctaattgt ctacatcctt gaccagatag ctgtaatgtt atttgatagt
      841 ttcctgtatc atgtttatct gcataatcaa atatccaaat atctccacgt ttccaaatat
      901 ggttataatt catcaactta gattcaactg atgaaaattc tttaaatcga caatgtaaaa
      961 aattccgaca aaaaaattcc aaatctctaa cgctttttaa cgtaatcctg acatagtcaa
     1021 ttgtcacttg taatcttgaa ctatcattat cagtcaaata accaaaatgt gttttcattt
     1081 cttgaaattt ttcaagagat aattttttct taccagattc atattgagat aacatagctt
     1141 gactaatacc tatacatttt gcaaattctt tctgctttaa gcctgatttc ttcctaaa
//
LOCUS       DATXQK010000121         1186 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.121, whole genome shotgun sequence.
ACCESSION   DATXQK010000121 DATXQK010000000
VERSION     DATXQK010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.121"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..1175
                     /locus_tag="VC394_001789"
     CDS             <1..1175
                     /locus_tag="VC394_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEP5995216.1"
                     /translation="AMLTVGFGSLDNKLTVDDESRDAPAGIAHFLEHKLFEDESGGDI
                     SLKFTQLGAETNAFTTFNQTSFFFSTASKFQENLELLQYFVLSANITDESVSREKKII
                     GQEIDMYQDDADYRAYSGILQNLFPKTSLANDIAGSKASIQKITKILLETHHTYFYQP
                     TNMSLFIVGDIDIDETFLAIQRFQTTLSYPDRKRVTVDPLHYYPVIKSSSVDMDVTIA
                     KLVVGFRGYLTLTQHSLLTYRIALKLFLSMLIGWTSKIYHTLYEDGKIDDSFDVDVEI
                     HHHFQFVLISLDTPEPIAMSNYIRQKLATIKISKEFTNEHLNLLKKEMYGDFIQSLDS
                     IEHLTHQFSLYLSDSDKETYFDIPKIIERLTLKDVVTIGKAFFEKADASDFTVFPK"
BASE COUNT          404 a          203 c          190 g          389 t
ORIGIN      
        1 cagctatgtt gacagtaggt ttcggttctc ttgacaataa gttgacagtc gatgacgaat
       61 cacgagatgc tccagcaggt attgcacact ttttagaaca taaactcttt gaagatgaat
      121 caggtggaga tatttcatta aagtttacac agctaggagc agagacaaat gcctttacaa
      181 ctttcaacca aacgagtttc ttcttttcaa cagctagtaa atttcaggaa aatttggaac
      241 tccttcaata ttttgttctt tctgctaata taacagatga atctgttagt agagaaaaaa
      301 aaataatcgg gcaagaaatt gatatgtatc aagatgatgc tgactaccgt gcatatagtg
      361 gaatccttca gaacttattt ccaaaaacaa gtttagctaa tgatattgct ggctcaaaag
      421 catccattca aaagatcacg aaaatattat tggaaacgca tcatacttat ttttaccaac
      481 ctactaatat gagtctattc attgtcggtg atattgatat tgacgaaact tttttagcta
      541 ttcaaaggtt tcaaaccaca ttatcctatc cagatagaaa aagggtaact gttgatcctt
      601 tacactacta tcctgtcatt aaatcatctt ctgttgacat ggatgttact attgccaagc
      661 tggttgttgg ttttcgaggg tatctgacct taactcagca ttcactatta acgtatcgta
      721 ttgccttgaa gctattttta tcaatgctta taggttggac ttctaaaatc tatcatacgc
      781 tatatgaaga tggtaaaata gacgattcat ttgatgtgga tgttgaaatt catcatcatt
      841 ttcaatttgt attaatatca cttgatacac cagagcctat cgcaatgtct aactatatcc
      901 gtcaaaaatt agccactata aagatatcaa aagagtttac aaatgagcac ttaaatctat
      961 taaaaaaaga aatgtatggt gactttattc agagtttaga ctctatagaa catttaaccc
     1021 atcagttcag tctatacctg tctgattctg ataaagaaac atattttgat attccaaaaa
     1081 ttatagaaag actcactcta aaagatgttg ttacaattgg aaaagcattt tttgaaaaag
     1141 ctgatgcttc agacttcaca gtcttcccaa aatagatgaa aatctg
//
LOCUS       DATXQK010000122         1167 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.122, whole genome shotgun sequence.
ACCESSION   DATXQK010000122 DATXQK010000000
VERSION     DATXQK010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.122"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            103..>1167
                     /locus_tag="VC394_001790"
     CDS             103..>1167
                     /locus_tag="VC394_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEP5995217.1"
                     /translation="MKIVQGVQLHLIKTKQFKTNHITFRFSGDLNQKTVAKRVLVAQM
                     LATANECYPTVRQFREKLARLYGASLSTNVLTKGLVHIVDIDITFIQDRYACNGEKIL
                     DEMIQFLKDILFSPLLSIAQYQPKVFETEKNNLINYIESDREDSFYYSSLKVKELFYC
                     NKNLQMSEYGSPELIAKETAYTSYQEFHKMLNEDQIDIFILGDFDDYRVVQLIHQFPL
                     DNRNKNLNFFHLQNSVNIIKESIEKRAVHQSILQLAYHFPSVFGQRDYYALVLLNGLL
                     GSFAHSRLFIKIREEEGLAYSIGCRFDSYTGLFEIYTGIDSQHRTKTLQLIIQELNAI
                     KMGRFSEQLIKKTRSMLLNNA"
BASE COUNT          417 a          185 c          188 g          377 t
ORIGIN      
        1 acactagaat cccgacaatt aagtcaaaaa aagttatcac tcacctaacc tgaataaaaa
       61 tggtataatc gtaaggatca cactatttat taaggaaaca ttatgaaaat tgttcaggga
      121 gtacaacttc atctgattaa gaccaaacag tttaaaacca atcatattac ctttcgtttt
      181 tcaggtgatt tgaaccaaaa aacagtagca aaaagagtct tagtggcaca gatgttagca
      241 acagctaatg agtgttatcc aactgttagg cagtttagag aaaaattagc acgactttat
      301 ggagctagct tgtcaactaa cgtgttaaca aaagggttag ttcatattgt ggatatcgat
      361 attactttta ttcaagatag atatgcttgc aatggggaaa aaatactaga tgaaatgatt
      421 cagtttttaa aagatatctt gttctcaccc ctattatcca tcgcccagta tcagcctaaa
      481 gtttttgaga ctgaaaaaaa taatttgatt aactacatcg agtcagatag agaggattct
      541 ttttactaca gctctcttaa ggtaaaagaa ttgttttatt gtaataagaa tctacaaatg
      601 tcagaatatg gtagcccaga actaattgca aaagagactg cctatactag ctatcaagaa
      661 tttcataaaa tgctcaatga agatcagatt gatattttca ttttaggaga ttttgatgat
      721 tatcgcgttg ttcagctaat tcatcaattt ccacttgata accgaaataa aaacctaaat
      781 ttctttcatt tacaaaattc tgtcaatatt attaaagaat ctattgaaaa aagagcagtt
      841 catcaatcta ttttacaatt agcttatcac ttcccttcag tttttggaca acgtgactat
      901 tatgctttag ttcttttaaa tgggttgtta ggctcttttg cacattcccg tctctttata
      961 aaaattagag aagaagaagg acttgcttat agtatagggt gtcgttttga tagttataca
     1021 ggtttatttg aaatttatac tggaattgac agtcaacatc gcactaaaac gttacaacta
     1081 atcatccaag aattaaatgc cattaaaatg ggacgattct cagaacaatt gattaaaaag
     1141 accagatcga tgttgttgaa taacgcc
//
LOCUS       DATXQK010000123         1161 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.123, whole genome shotgun sequence.
ACCESSION   DATXQK010000123 DATXQK010000000
VERSION     DATXQK010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1161
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.123"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(99..1013)
                     /gene="hasC"
                     /locus_tag="VC394_001791"
     CDS             complement(99..1013)
                     /gene="hasC"
                     /locus_tag="VC394_001791"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     HasC"
                     /protein_id="HEP5995218.1"
                     /translation="MTKVRKAIIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVEE
                     ALKSGIEEILVVTGKAKRSIEDHFDSNFELEYNLQAKGKNELLKLVDETTAINLHFIR
                     QSHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITNASAKPLTKQLMEDYDKTHASTI
                     AVMKVPHEDVSSYGVIAPQGKAVKGLYSVDTFVEKPQPEDAPSDLAIIGRYLLTPEIF
                     DILERQTPGAGNEVQLTDAIDTLNKTQRVFAREFKGNRYDVGDKFGFMKTSIDYALEH
                     PEVKEDLKNYIIKLGKALEKSKVPTHSK"
BASE COUNT          317 a          231 c          229 g          384 t
ORIGIN      
        1 ttgtataaca caaaactttt atattttgga acgttgacaa ttttcaagat ataaacttaa
       61 gttgctataa acaaggaaaa aagtaaagta ccaacacatt actttgaatg tgttggtact
      121 ttactttttt ccaaagcttt tcctagtttg ataatgtaat ttttcaagtc ctctttgacc
      181 tctgggtgtt ctaaggcata gtcgatagat gttttcatga atccaaattt atccccaaca
      241 tcgtaacgat tacctttaaa ttctcgtgca aagacacgct gagttttatt gagggtatcg
      301 atagcatctg tgagttgcac ttcgttacct gctccagggg tctgtctttc caaaatatca
      361 aaaatttcag gggttaggag gtaacgacca ataatagcca aatcactagg cgcatcttct
      421 ggttgtggtt tttcaacaaa ggtgtctaca ctgtaaaggc ccttgacagc cttgccttga
      481 ggagcgataa ccccatagct agacacatct tcatgaggaa ctttcatcac agcgatagtg
      541 gatgcatgcg tcttgtcata gtcctccatg agttgtttgg tgagaggttt agcggatgca
      601 tttgtaatgt ccattaagtc atctccaagc atgaccacaa agggttcatt gcccacaaag
      661 gctttggctt gtaagacagc atctcccagt cctcttgggt ggctttgacg gataaaatga
      721 aggttaatgg cagtggtttc atccactaat ttcaacagtt catttttccc cttagcttgg
      781 aggttgtatt ctaattcaaa gtttgaatca aaatggtcct cgatagagcg tttagctttt
      841 ccggtcacca caaggatttc ctcgatgcca gattttagcg cttcttcgac gataaactgg
      901 atggttggtt tatcaacgat gggcaacatc tctttggcaa gagctttggt agcaggtaaa
      961 aaacgtgttc ctagacctgc agcaggaata atggcttttc tgactttggt catcagtaac
     1021 tcctcacttt ttataatcta tggcttagtg acattcgttt tcagaacgga attcatttga
     1081 catcatgtta cgttttcata tgatttttcg actatttcat agtgctcaat cataccacca
     1141 actgcagttc ttttaaagta a
//
LOCUS       DATXQK010000124         1149 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.124, whole genome shotgun sequence.
ACCESSION   DATXQK010000124 DATXQK010000000
VERSION     DATXQK010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.124"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..277)
                     /locus_tag="VC394_001792"
     CDS             complement(<1..277)
                     /locus_tag="VC394_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEP5995219.1"
                     /translation="MLVLYVSSKLIKRRRMKFMYSALLGNPVEHSISDELFEYLNHNI
                     GVLDEYKHKKILVDSVCLEDKIKHLINDPKCIGLNITLPYKREVMRYI"
     gene            complement(346..417)
                     /gene="shp3"
                     /locus_tag="VC394_001793"
     CDS             complement(346..417)
                     /gene="shp3"
                     /locus_tag="VC394_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone SHP3"
                     /protein_id="HEP5995220.1"
                     /translation="MKKISKFLPILILAMDIIIIVGG"
     gene            497..>1149
                     /gene="rgg3"
                     /locus_tag="VC394_001794"
     CDS             497..>1149
                     /gene="rgg3"
                     /locus_tag="VC394_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985634.1"
                     /note="Regulator gene of glucosyltransferase 3; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg3"
                     /protein_id="HEP5995221.1"
                     /translation="MKSKLGATLRKVRKGKQISLCFVADEHLSKSQISRFERGESEIS
                     CIRLINILDKLHITLDEFLILHDEDYTNSETFANLVQYIRKQYSSQNINNIACLLSDT
                     SDYTLNSFEKTMVKSILHTMDSSIIPSDDELLQLTDYLFKVEKWGYYEILLLGNCVRT
                     INYNSYFLLTKEMLNNYIYSSRNKTNKRIVTQLAINCFILSINKEEFSNCSYLIDEI"
BASE COUNT          404 a          199 c          158 g          388 t
ORIGIN      
        1 caatatacct cattacctct cttttataag gtaaagttat atttaatcca atacattttg
       61 gatcatttat cagatgtttg attttatctt ctaaacacac tgagtctacc aaaatttttt
      121 tatgtttata ttcatccaga actcctatgt tgtgatttag atattcaaat aattcatctg
      181 atatagaatg ttcaactgga ttaccaagca atgcagaata cataaatttc atcctcctac
      241 gtttaattag tttactacta acataaagta caagcattac acagagctaa ccattaccta
      301 taagtcttta gagtatctaa agacttaggt gtacacctaa gtaacttacc caccaacaat
      361 aatgataata tccattgcta aaattaaaat cggcaaaaat tttgaaattt tcttcataag
      421 cggacttcct ttcagttttt atttattgta caataaaaaa aaacggaatt gatttttgtt
      481 gtgaaagtgg gaaaatatga aatcaaaact tggtgccaca cttagaaaag ttcgaaaagg
      541 aaagcaaatt agtctgtgct tcgtggcaga tgaacacctt tcaaaatcac aaatctctcg
      601 ttttgagcgc ggggagtcag agatatcgtg tatccgactg atcaatattc tagacaaatt
      661 acacattact ttggatgagt ttcttatttt acatgatgaa gactatacca attccgaaac
      721 atttgctaac ctggtacagt atattcgtaa gcaatattca tcacaaaata tcaataatat
      781 agcatgttta ctttccgaca cttctgatta cactctgaat tcttttgaga aaacaatggt
      841 aaagtccatt ttacacacaa tggattcaag catcattcca tcagatgatg aattacttca
      901 actaacagat tatctcttta aagttgaaaa atggggctac tatgaaatcc ttttgttggg
      961 taactgtgta cgaacaatta actacaactc ctacttccta ttgacaaagg agatgttaaa
     1021 taattacatt tactcttcac ggaacaaaac taataaacga attgtcactc agcttgctat
     1081 aaattgtttc attcttagta tcaataagga ggaattttct aattgctcct atttaattga
     1141 tgagataaa
//
LOCUS       DATXQK010000125         1127 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.125, whole genome shotgun sequence.
ACCESSION   DATXQK010000125 DATXQK010000000
VERSION     DATXQK010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.125"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..419
                     /locus_tag="VC394_001795"
     CDS             <1..419
                     /locus_tag="VC394_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiopeptide-type bacteriocin biosynthesis
                     protein"
                     /protein_id="HEP5995222.1"
                     /translation="ERLVCATYLVLYYLNHFFKDKETRRSFLFENYTGKYKKDFKNLP
                     IDLPIEYMKILNGVTSALDRYDFFQEMDRYLMSYMKEYNRFGGTNDIYNTKFNLVGSF
                     LHLSMNRLNGIDREFEEKVYCFAYYTLNAQQYIEWE"
     gene            425..1114
                     /locus_tag="VC394_001796"
     CDS             425..1114
                     /locus_tag="VC394_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HEP5995223.1"
                     /translation="MLKIQNLKKSYGKRTILNNVNMNIPKGKVYALIGPNGAGKSTIM
                     KILTGLVSKTSGSIIFEGREWSRRDLRKIGSIIEEPPLYKNLSAYDNMKVVTTMLGVS
                     ESAILPLLNKVGLGNIDKRPVKQFSLGMKQRLGIAISLINSPKLLILDEPTNGLDPIG
                     IQELREIIESFKSEGMTIMISSHILSEVEHLADFIGFIYEGKIVLEKEYDGSENLEEL
                     FNNQILFEKRR"
BASE COUNT          411 a          137 c          214 g          365 t
ORIGIN      
        1 atgaaagatt agtttgtgct acttacttgg ttctatatta tctaaatcac tttttcaaag
       61 ataaagaaac aaggcgctct ttcttatttg agaattatac cggaaagtat aagaaagatt
      121 tcaaaaattt gccaattgat ttacctattg agtatatgaa gattttgaat ggagtcacct
      181 cagctcttga tagatatgat tttttccaag aaatggatag atatctcatg tcttatatga
      241 aagagtataa tcgatttggt ggtacaaatg atatctataa tactaaattc aatttagtag
      301 gaagtttttt acatctatca atgaatcgat taaacgggat tgatagagag tttgaagaga
      361 aagtgtattg ttttgcttac tatacactaa acgctcagca atatatagaa tgggagtaac
      421 taatatgttg aaaatacaaa atctaaaaaa atcttatggt aagagaacca tattgaataa
      481 tgtcaatatg aatattccta agggaaaagt gtatgcttta atcggtccaa atggtgctgg
      541 aaaatcaact attatgaaaa ttttgacagg cttagttagt aagacaagtg gttctattat
      601 ttttgaaggt agagaatggt cacgtcggga tctgcgaaaa atcgggagta ttattgaaga
      661 accaccactt tataaaaatt tgagtgctta cgataatatg aaggtagtta caacaatgct
      721 tggtgtttca gaaagcgcta tacttccatt attaaataaa gttggtctag gaaatattga
      781 caagagacca gtaaaacaat tttctcttgg aatgaagcaa cggttaggta tagctatctc
      841 tttaataaat tcacctaaat tacttatatt agacgaacct actaatggct tggacccaat
      901 tggaattcaa gaattaaggg aaattataga gtcatttaaa tcagaaggaa tgacaattat
      961 gatttcaagc catatactgt cagaagttga acatctagct gattttattg gatttatcta
     1021 tgaaggaaag attgttctgg aaaaagaata tgacggctct gagaatcttg aagagttatt
     1081 caataatcaa attttatttg aaaagaggag gtaggggcta tggtaca
//
LOCUS       DATXQK010000126         1092 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.126, whole genome shotgun sequence.
ACCESSION   DATXQK010000126 DATXQK010000000
VERSION     DATXQK010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.126"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..1077)
                     /locus_tag="VC394_001797"
     CDS             complement(<1..1077)
                     /locus_tag="VC394_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEP5995224.1"
                     /translation="MFDFANNTFLAGLNPASLSLVAVYQSLESVIGVLFNLFGGVIAD
                     SFKRKKIIITTNILCGTACLVLSFLTKEQWLVYAIVLTNVILAFMSAFSSPSYKAFTK
                     EIVKKDSISQLNSLLETTSTVIKVTVPMVAIFLYKLLGIHGVLLLDGLSFLIAALLIS
                     FILPVNDEVVIKEKVTIREIFNDLKIGFKYVYSHKSIFIITVLSALVNFFLAAYNLLL
                     PYSNQMFGEISTGLYGTFLTAEAIGGFIGAILSGFVNKELSSMRLILFLSLSGLMLML
                     APPFYIMFHNAIILALSPALFSLFLSIFNIQFFSLVQKDVDNDFLGRVFGIIFTITIL
                     FMPIGTGFFSVALNPNNSFNLFIIG"
BASE COUNT          435 a          182 c          160 g          315 t
ORIGIN      
        1 accaataata aaaagattaa agctattgtt aggatttaat gccaccgaga aaaagccagt
       61 cccaataggc ataaaaagaa tagttattgt aaagataata ccaaacactc tccctaaaaa
      121 atcgttatca acatctttct ggacaagaga aaaaaattga atattaaaga tggaaagaaa
      181 taaactgaat aaagctggtg acaaagctaa aataattgca ttatgaaaca taatataaaa
      241 tgggggagct aacattaaca tcagtcctga taaagataga aacaatatca atctcatact
      301 tgataattct ttattcacaa aaccacttaa tattgcccca ataaatccac caattgcttc
      361 tgctgttaaa aaagtgccat aaagtccagt tgaaatttct ccaaacattt gattactata
      421 gggcaacaat aaattataag cggctagaaa aaaattaaca agtgcagaaa gaacggtaat
      481 aataaagatg gacttatggc tgtaaacata tttaaatcct atttttaaat cgttaaatat
      541 ttctctgatt gtcaccttct cctttatcac cacttcgtca ttaacaggta aaataaagga
      601 aattagtaaa gcagctatta gaaatgatag tccatccaat agtaatacac cgtgtatacc
      661 aagtagctta tataagaaaa ttgctaccat aggaactgtt actttgatta cagtacttgt
      721 tgtctctagc aatgaattaa gttgtgatat actatctttt ttaacaattt cttttgtaaa
      781 tgctttatag gatggactag aaaaagcact cataaatgcc aagataacat tagttagtac
      841 aatggcataa accaaccatt gctcttttgt taggaacgag agtactaagc aagctgtgcc
      901 acataagata tttgtagtaa taataatttt tttccgcttg aaactgtctg caatgactcc
      961 accaaataaa ttaaatagaa cacctatgac actttctaga gactgatata ctgcaactaa
     1021 tgatagagac gctggattaa gcccagcaag aaaagtattg ttagcaaaat caaacataac
     1081 atcgccaatt cg
//
LOCUS       DATXQK010000127         1020 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.127, whole genome shotgun sequence.
ACCESSION   DATXQK010000127 DATXQK010000000
VERSION     DATXQK010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.127"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            54..828
                     /locus_tag="VC394_001798"
                     /pseudo
     CDS             54..828
                     /locus_tag="VC394_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001871253.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT          336 a          205 c          194 g          285 t
ORIGIN      
        1 atgttcatct gatatactag aattccccaa ttcagtatgg aaagcagatg aacatgtcta
       61 atattaattc taccagaaag tcatcctatt ctcatctttc agccacagaa cggggtgaaa
      121 ttgctgctta tcttaaaatg ggaaagaaac ccgttgagat tgctcgactt ctggggcgtc
      181 actgttctac aatctgccga gaaataaaac gtggttcagt agatcaggta aaggataaga
      241 atggaaaaca aaccttcttc aacgcctatt tcgctgatag tgggcaacgt gtctattaaa
      301 ccaatcgatt tgcctaaggt ggttagtatc agaaaacgtt ctaagaaagt catgaagacg
      361 aataagaaga ctttaggtaa atctatcgaa gaacgtccag aatatattaa tgatcgttct
      421 gaatttgggc attgggagat tgatttggtt ctcggcaaga aaaccaaaag tgaagctgtt
      481 atgttgactt tggtagagcg ccagacacgc tatgcactag gagttaagct agaagacaag
      541 cagtcccaaa ccattaaccg tgctgtcacg catctcatca gtcagtatcc tattgcatcc
      601 atcacagcgg ataatggttc tgagtttagt ttactctcag acttaaaagc tgttgacgtt
      661 tactttgcac atccctattc ttcacatgag agaggaacaa gcgagaactt caatggcctc
      721 ctcagagaat atgtccctaa aggagtctca cttaatccac taacctctga agaacttgac
      781 aatgatatta ctgccatcaa tgagcgccta agacgacttc ttcaatagca atcctcaaaa
      841 ttcctgtttg agctatcccg aacagcttaa cctctggaac tctagttatc tatgaactcg
      901 ttgcacttga cttgacaact tgcggtttaa aaaaaatcaa atttttttaa aaaaagtata
      961 gacaaaaaaa atcaatctgc tataatagta agagtgttaa ggagatatcc tccaaaactt
//
LOCUS       DATXQK010000128         1016 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.128, whole genome shotgun sequence.
ACCESSION   DATXQK010000128 DATXQK010000000
VERSION     DATXQK010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.128"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            7..>1016
                     /locus_tag="VC394_001799"
     CDS             7..>1016
                     /locus_tag="VC394_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP5995225.1"
                     /translation="MKLKLSVIGLFFCLIAAIGVISISDTQKPVPLPIDGAFSIQGKS
                     SLSSDEVYKKISNLSKTKNVNIYKPIVRNSGQLTYINFKNTNYEQLKPAPITGMYYTL
                     GKIDSSSLKVLTASGLKLVYAAYPWYIGGILQFDGTLRLLLMLSIYLTLLVVLFVVRT
                     RQIKEGVIRHSLGLPIYNLRRDYVISFTFELVMAGLLMIAYSYFWGSGLFTYSSKLFF
                     SLILTNLIIFQIVDIITFILFWLTIRVEKPIEIIKNKAKNSFLFIVWLVIISIIIIVS
                     GIFLQETKASQSRINRQISQLSSWNKVRNWKRLELLGIENGSINNGEINEPESQYIQI
                     ATA"
BASE COUNT          345 a          133 c          175 g          363 t
ORIGIN      
        1 ttgtttatga aattaaaatt atctgtcatt ggattattct tttgcttaat tgctgcgata
       61 ggtgttatca gtattagtga tactcaaaaa ccggtacctt taccaattga tggtgccttt
      121 tcaatccaag gaaaaagtag cctttctagt gacgaagttt acaaaaagat tagtaatcta
      181 tcaaaaacga aaaacgttaa tatttacaag cctatagttc gtaactcagg tcagttgact
      241 tatatcaatt ttaaaaatac aaattatgaa caattaaaac ctgcaccgat aacaggcatg
      301 tattatacct taggtaaaat agattcaagt agcttaaagg tacttacagc ttcaggtttg
      361 aaacttgttt atgcagccta tccttggtat atagggggga ttttacaatt tgatgggact
      421 ttaaggttgt tattgatgct atcaatttac ttaacattat tagttgtttt atttgttgtt
      481 agaacgaggc agattaaaga aggagtgata agacattctt taggattgcc tatatataac
      541 ttaagaagag attatgtcat ttcttttact tttgaattgg ttatggctgg tttattaatg
      601 attgcctaca gctatttttg gggaagtggc ctctttactt acagttctaa gttatttttc
      661 tctctaattt taactaatct tataatattc cagattgttg acattatcac ttttatttta
      721 ttttggctaa ctattagggt tgagaaacct attgaaatta taaaaaataa agcaaaaaat
      781 agttttcttt ttattgtatg gcttgtaatt atttctatta taataatcgt ctcaggaatc
      841 tttttacaag aaacaaaggc tagtcaatca aggataaata gacagataag tcaactatct
      901 tcatggaaca aagtaagaaa ctggaaaaga ctagagttgt tggggattga aaatggctct
      961 attaataatg gggaaataaa tgagccagaa tcacaatata tacagatagc gacagc
//
LOCUS       DATXQK010000129          997 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.129, whole genome shotgun sequence.
ACCESSION   DATXQK010000129 DATXQK010000000
VERSION     DATXQK010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.129"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..>997
                     /locus_tag="VC394_001800"
     CDS             <1..>997
                     /locus_tag="VC394_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HEP5995226.1"
                     /translation="LTLLLTVILYANSLNLPLKFISLLFVVSTLLIVFLPKKLEVATI
                     IFILVFGTISAIISPINDIPDEYVHYSRTVYISEGDINLTNNNKKLRISKDVDKLIKQ
                     SGKTFITSNLKATKHSTREYSYPYIKGTNAYYSFSYIPQALGILVGNALDLPILLTYY
                     FGRLCNLISYAMLAFIAIKLSGSFKQVIAVVTLLPMNIYLAASFNQDGFAIGLVLVTI
                     GLFINLLSSKDKSNYNTKFFLYLVLCGLLVLSKFTYFLLVCLPLFIPNEKFGKNTKLV
                     ILKKLGGLLLIFLFAAMWFRLYGQVKTPYVADFLKEVNVSQQVKNMLESPIVYSSI"
BASE COUNT          324 a          156 c          146 g          371 t
ORIGIN      
        1 tttgacatta cttctgacag ttattttata tgccaatagt ttaaatctac ctttaaaatt
       61 tattagtctt ctttttgtgg tgagcacttt actgatagtg tttttaccaa aaaagttaga
      121 ggttgctacg atcattttta ttttagtatt tggaacgata tctgctatca tatcacctat
      181 aaatgatatt cctgatgaat atgttcatta ttcaaggaca gtttatatct cagaaggtga
      241 tattaatctt accaacaata acaaaaagtt aagaatatcc aaagatgttg ataagcttat
      301 taaacagtca ggaaaaacat ttataacatc aaacttgaaa gcaactaaac acagcacaag
      361 agagtatagc tatccttaca tcaaaggaac taatgcatat tatagttttt cttatatacc
      421 acaagctttg ggtattttag taggaaatgc gctggattta cctatacttt taacatatta
      481 tttcggaaga ttgtgcaacc ttattagcta cgctatgctt gcttttatag ctatcaagtt
      541 gtctggttct tttaaacaag tgatagctgt tgttacttta ctacctatga acatttattt
      601 agcagcatct tttaatcaag acggctttgc tatcggttta gttttagtaa caattggatt
      661 gtttattaac ttgctctcat caaaagataa atctaactat aataccaaat tcttccttta
      721 tttagttcta tgtgggttac tagttctttc aaaattcaca tactttttat tagtatgcct
      781 tccattattt attcctaatg aaaaattcgg aaaaaatacc aagttagtca tcttgaaaaa
      841 attgggagga ctattactca tttttctttt tgctgcaatg tggtttagac tttatggaca
      901 ggttaaaacc ccatatgtcg cggattttct aaaagaagta aacgttagcc agcaagttaa
      961 aaatatgcta gaatccccta tagtctatag cagtatt
//
LOCUS       DATXQK010000130          976 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.130, whole genome shotgun sequence.
ACCESSION   DATXQK010000130 DATXQK010000000
VERSION     DATXQK010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..976
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.130"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            17..631
                     /locus_tag="VC394_001801"
     CDS             17..631
                     /locus_tag="VC394_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011185103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEP5995227.1"
                     /translation="MFKKENLKQRYFNFGLVALALTILAIIFAFSSKNADTKSYAKKS
                     ESKMVTIDKAPKNNHAITKEESKDKSKSIASEPIPTVENSVAPTVTEEAPVVQQEVTQ
                     TVQQVSSVAYNPNNVVLSNGNTAGIVGSQAAAQMAAATGVPQSTWEHIIARESNGNPN
                     AANASGASGLFQTMPGWGSTATVEDQVNAALKAYSAQGLSAWGY"
BASE COUNT          338 a          162 c          191 g          285 t
ORIGIN      
        1 agtgaggtat tattgaatgt ttaagaaaga aaatttaaaa caacgttatt ttaattttgg
       61 attagtagcg ttagctctaa caatattagc catcattttt gccttctcaa gtaaaaatgc
      121 tgatactaag tcttatgcta agaagtcaga aagtaaaatg gtaacaatcg acaaggctcc
      181 aaaaaataat catgctatta ctaaagaaga aagcaaagat aaatcaaaga gcattgcttc
      241 ggagcctatt cccacagtag aaaactctgt agctccgaca gtaacagagg aagcaccggt
      301 tgttcagcaa gaagtgactc aaactgttca gcaggtatct tcagtagcct ataatccaaa
      361 caatgtggta ctttccaatg gaaatactgc tggtattgta ggaagtcaag cggcggcaca
      421 gatggcagca gcaacaggtg ttccacaatc aacttgggaa catataattg cgcgtgaatc
      481 taatggaaat cctaacgcag ctaatgcttc tggggcatca gggttgttcc agacaatgcc
      541 aggttggggt tctacagcaa cggttgaaga tcaagtcaat gcagccttga aagcctatag
      601 tgcacaaggt ttatcagctt ggggttacta aataaacata gctggaagaa ataaaaccta
      661 ttgttagtat ttactaacaa taggtttttg gtatttaatt gtctttatga tttgtagtga
      721 tgaattagtc tcaaaacaaa acattgcctt atcagggaat atagctcgta ttatttagga
      781 aaactgacca gcacttaagt gcctctgata tttaaggcag ttatactgat aacaagcagg
      841 aaaaaaactc ttaaatgttt ataagatcat tagcaatttt cagtatttta cactactaca
      901 cttttgagtt ttaacaattt actagaagtt gtttctaagg ggaaacaaca tagcttgata
      961 gctatctatt agtcat
//
LOCUS       DATXQK010000131          960 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.131, whole genome shotgun sequence.
ACCESSION   DATXQK010000131 DATXQK010000000
VERSION     DATXQK010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..960
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.131"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            56..481
                     /locus_tag="VC394_001802"
     CDS             56..481
                     /locus_tag="VC394_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002840511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEP5995228.1"
                     /translation="MNKSIGKLITYLSRCIQQELKNVVVPFDISVGEEPYYMALTKQD
                     GLTQDELTKLVKVDKAATTRMVKSLEQKGYITRETDPKDKRNKKIYLTERGRLKYEPL
                     SRALKEFNQKLTSEWTDEQYKIVYQSLEMLQKKFEIDNK"
     gene            756..854
                     /locus_tag="VC394_001803"
                     /pseudo
     CDS             756..854
                     /locus_tag="VC394_001803"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          400 a          132 c          169 g          259 t
ORIGIN      
        1 agtatttttt ggaatgtgat attggacaaa tttttaagaa gaaaagaggc ataaagtgaa
       61 caaaagcatt ggaaaactta taacttattt atcaagatgt attcagcaag aattaaaaaa
      121 tgtagttgtt ccatttgata taagtgtcgg agaagagcct tattacatgg cacttacaaa
      181 acaggatgga ttgactcagg atgaattaac aaaacttgtg aaagtagata aggctgctac
      241 gactcgaatg gttaaatcat tagagcaaaa gggatatatc acacgagaaa cagaccctaa
      301 agataagaga aacaaaaaaa tatatcttac agaaagggga cgattaaaat atgaaccttt
      361 aagtagagca ttaaaagaat ttaatcagaa gttaacatca gaatggacag atgaacaata
      421 taaaatcgtt tatcaaagtc tggaaatgct acaaaaaaaa tttgagattg ataataaata
      481 agttttaatt tattgcccct ctacacatcg ggtcaacttg ccacgaactt tcacaactaa
      541 atacccaccg ctaaaacagc ggcacaccaa gcaggaaatc tgaaaaaggt ctcctggtat
      601 ggatacaata aataataagt aatcatagga ggattttaaa ttagctagac actacgaaga
      661 agacttcaaa aggcaaatag tagaaatctt taatcaaggt aacaacacat ataaaaaatt
      721 gggagaagaa tacgatatag caccgtcaat aatcagagta tgtttattgg aacaataacc
      781 taagggttaa tggatcatta ggttacttga caccagttaa atataggcaa tcaagattaa
      841 ctagtagtta ttagttaatc taaaattagt acgttcgatt ttagattgta caaataggtg
      901 ttgacaatcc acactaagct gaaaaacgat tagaaataaa aatctattcg tttttttatt
//
LOCUS       DATXQK010000132          953 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.132, whole genome shotgun sequence.
ACCESSION   DATXQK010000132 DATXQK010000000
VERSION     DATXQK010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.132"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..406
                     /gene="tilS"
                     /locus_tag="VC394_001804"
     CDS             <1..406
                     /gene="tilS"
                     /locus_tag="VC394_001804"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEP5995229.1"
                     /translation="SFKITKIVPKTELVKDEKMLEYDSNLCYRDYCFSFMPKSNEDQG
                     QVSIPLFSLSSIKLRSRQSGDYISFGHFSKKIRRLFIDEKFTIAERQNAIIGEQDEQI
                     IFVLIGNKTYLRKACKHDIMLAKLYIDKLEKG"
     gene            411..953
                     /gene="hpt"
                     /locus_tag="VC394_001805"
     CDS             411..953
                     /gene="hpt"
                     /locus_tag="VC394_001805"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012961310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEP5995230.1"
                     /translation="MLEQDIQKILYSENDIIRKTKKLGEQLTKDYQEKNPLMIGVLKG
                     SVPFMAELMKHIDTHVEIDFMVVSSYHGGTSSSGEVKILKDVDTNIEGRDIIIVEDII
                     DTGRTLKYLRDMFKYRKANTIKIATLFDKPEGRVVKIEADYVCYNIPNEFIVGFGLDY
                     AENYRNLPYVGVLKEEVYSK"
BASE COUNT          365 a          115 c          166 g          307 t
ORIGIN      
        1 ctcttttaaa attacaaaaa tcgttcctaa gactgagtta gttaaagatg aaaaaatgtt
       61 agaatacgat agtaacttat gctacagaga ttattgtttt tcatttatgc caaaaagtaa
      121 cgaagatcaa ggacaggtaa gtataccttt atttagttta tcgtctatta aattaagaag
      181 tcgccaatct ggagattata tttcttttgg tcacttttca aaaaaaataa gacgtttatt
      241 tatagatgaa aaattcacaa tagctgaacg ccaaaatgct attattggag agcaagatga
      301 gcagattata tttgtattga taggtaataa aacttatttg agaaaagctt gtaaacatga
      361 tataatgttg gctaaactgt atattgataa attagaaaaa gggtgacagc atgctcgagc
      421 aagatattca aaaaattctt tactctgaaa atgatattat tcggaaaact aaaaaattag
      481 gagaacaatt aacaaaagat tatcaagaaa aaaatccgct tatgattggt gtattaaaag
      541 gatcagttcc ttttatggca gaactaatga aacacatcga tactcatgtt gaaattgatt
      601 ttatggtagt atcaagttat catggcggta catctagcag tggtgaagtt aaaattttaa
      661 aagatgtaga taccaatatt gaaggaagag atatcataat tgttgaagat attattgaca
      721 ccggtcgcac cttaaaatat ttacgagata tgtttaagta ccgtaaagct aatactatca
      781 aaattgcaac actgtttgat aaacctgaag gtcgtgttgt taaaattgaa gcagattatg
      841 tttgttacaa tattccaaat gaatttatcg taggctttgg tttagattac gcagaaaatt
      901 atcgcaatct tccttacgta ggtgtattaa aagaagaagt ctattcaaaa tag
//
LOCUS       DATXQK010000133          900 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.133, whole genome shotgun sequence.
ACCESSION   DATXQK010000133 DATXQK010000000
VERSION     DATXQK010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.133"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            46..255
                     /locus_tag="VC394_001806"
     CDS             46..255
                     /locus_tag="VC394_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017645614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5995231.1"
                     /translation="MRPKKYPYSQKNYPAPKLHNVIETDNQFLIDDKRIHYVIEDSVK
                     TKTLGDGYVEVTLSIIAKSFTKSQG"
     gene            complement(244..630)
                     /locus_tag="VC394_001807"
     CDS             complement(244..630)
                     /locus_tag="VC394_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5995232.1"
                     /translation="MFDYFKLYIIVLETFVETKPDDAYSLFDNLSLNSEFVKFSKDLD
                     KNIVISGTLEVIDNLLDDCLIKGKRRPTKDITFYFFDGVTTTGYLYLQSLKDNNFSSR
                     LKNVLKEEGIPLTPTSITRTIAKLTL"
     gene            704..>900
                     /locus_tag="VC394_001808"
     CDS             704..>900
                     /locus_tag="VC394_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEP5995233.1"
                     /translation="MTKKQRLKQEHLKPKKRLREERLKREYTKMYMADLIGLKNRKGY
                     SEKEDGLQPFKDYEMAIISQK"
BASE COUNT          330 a          137 c          160 g          273 t
ORIGIN      
        1 ccaatcattt tatttaaatt ataccacaga aagggggtgg aatagatgcg ccccaaaaaa
       61 tatccgtata gccaaaaaaa ttatccagcg ccgaagcttc ataacgtcat tgaaaccgat
      121 aatcagttct taatagatga taaaaggatt cattatgtta ttgaagattc tgtaaagaca
      181 aaaacccttg gcgatggcta tgttgaagta acactttcca taattgccaa gagttttaca
      241 aaatcacaag gttagtttgg cgatagtcct tgtgatagaa gttggtgtaa gagggatacc
      301 ttcttcttta aggacattct ttaaacggct cgaaaaatta ttatctttaa gactttgaag
      361 atataagtaa ccagttgttg ttacgccatc gaaaaaatag aaagtaatat cttttgttgg
      421 gcgacgtttt ccttttatca agcaatcatc aagtaaatta tcaattactt ctaaagtgcc
      481 tgatattaca atgtttttat ctaagtcttt agagaatttt acaaattcac tgttaagact
      541 tagattatca aataagctat aagcgtcgtc tggtttggtt tccacgaagg tttccaagac
      601 aattatataa agtttgaaat agtcaaacat cacattcacc tcctttctgc ccatattata
      661 gcagattgga ggaactaaaa acagatagaa aggaggtggg ggaatgacga aaaaacaacg
      721 tttaaaacag gaacatctca aacctaaaaa gcgtttaaga gaagaacgtt taaaacgtga
      781 gtacacaaaa atgtatatgg ctgatttaat tggtttaaaa aatagaaaag gttactctga
      841 gaaagaagat ggtttacagc cttttaaaga ttatgaaatg gctatcattt ctcaaaaatt
//
LOCUS       DATXQK010000134          881 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.134, whole genome shotgun sequence.
ACCESSION   DATXQK010000134 DATXQK010000000
VERSION     DATXQK010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.134"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..747
                     /locus_tag="VC394_001809"
     CDS             <1..747
                     /locus_tag="VC394_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HEP5995234.1"
                     /translation="FKEGISLYYIGEYLAECLLIEHSDTLLIHAAAVYNPLSGHSILL
                     LGDKGAGKTTVAIRLCIEHGYHLIGNDQVIFGSNSGILLTYAGTSFFKIRRTAVLSDN
                     LLFKLFSKFFNRSLQFNKASWDDKISIFPKELGIRTCNFSTEISKIYYIKTDKKEKQI
                     YHSIWSDSLASLYLNELLGRHISGQVACFQSDNGKYFSTMPLINYEKNNRVREQIVKG
                     IFAKQKLFKITAATPAKIAEAIIENVKKNS"
BASE COUNT          318 a          108 c          168 g          287 t
ORIGIN      
        1 tttaaagaag gaatttcatt atactacatt ggagagtact tagctgaatg tctcttgatt
       61 gaacatagtg acaccttatt aattcatgca gcagcagttt ataatccttt atctggccat
      121 tcaattttac tattaggaga taaaggtgct ggaaaaacta cggttgcaat aaggctgtgt
      181 atagaacatg ggtatcacct tattggtaat gaccaagtaa tatttggttc gaactctgga
      241 atactactaa cttatgcagg gacatctttc tttaaaataa gaaggacagc tgttttgtcg
      301 gacaatttac tgtttaaatt atttagcaaa ttttttaacc gttcattaca atttaataag
      361 gcaagttggg atgataaaat ttcaattttc ccaaaggagt tgggaatacg aacttgtaat
      421 ttttctactg aaatctcaaa aatttattat ataaagacag acaagaaaga aaagcaaatt
      481 tatcattcaa tttggagtga tagtttagca agtctatatc taaatgagct attgggaagg
      541 catatttctg gacaggttgc atgttttcaa tcagataatg gaaaatattt ttctacaatg
      601 cctttgataa attatgaaaa aaataatcgg gtacgagaac agatagtgaa gggtattttc
      661 gctaaacaaa aattatttaa aattacagca gctacgccag ctaagattgc agaagcgatt
      721 atagaaaatg ttaagaaaaa ctcatagtgt gtgtgaattt ggggattaat atgaagtatt
      781 gaaaaaggag taagagtaat tatgtttgtg ataggcattc caactttaaa tgaggcagat
      841 aatatcagta gattagttaa acaaattgat gagtatgctg t
//
LOCUS       DATXQK010000135          871 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.135, whole genome shotgun sequence.
ACCESSION   DATXQK010000135 DATXQK010000000
VERSION     DATXQK010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.135"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..674
                     /locus_tag="VC394_001810"
     CDS             <1..674
                     /locus_tag="VC394_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018373772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEP5995235.1"
                     /translation="AIDDDKEILKLMKTALEIENYHVITCQEIELPIVFDDFKGYDLI
                     LLDIMMPNISGTEFCYKIREEVHSPIIFVSALDGDNEIVQALNIGGDDFIVKPFSLKQ
                     FVAKVNSHLKREERAKIKNEAEERVKRSFPPIEIYLEERMLYIDKQPLFLTYREYEIL
                     ELLSRHPYKVFTKEEIYEQVYSDEASALFHSISEYIYQIRMKFSSFGINPIKTIRGIG
                     YKWDV"
BASE COUNT          310 a          105 c          168 g          288 t
ORIGIN      
        1 tagccataga tgatgataaa gaaattctga agcttatgaa gactgctttg gagattgaaa
       61 attaccatgt tataacctgt caagagatag aattaccaat agtttttgat gattttaaag
      121 gatatgattt gatcttattg gatatcatga tgcctaatat aagtggaact gagttttgtt
      181 ataaaattcg ggaagaagtt cattctccaa ttatttttgt tagcgcttta gatggcgata
      241 atgaaattgt ccaagcttta aatatagggg gagacgattt tattgtgaaa ccatttagct
      301 taaaacaatt cgtagctaaa gttaactctc atttgaagag agaagagaga gcaaagataa
      361 agaatgaggc tgaggagaga gtgaagcgca gttttccacc tatagaaatc tatctagagg
      421 aacgtatgtt atatattgat aaacagccgt tattcctgac ttatagagag tacgaaattt
      481 tagaattact gtcacgtcat ccgtataaag ttttcacaaa agaagagata tatgaacaag
      541 tatatagcga tgaagcttca gcattgtttc attctatttc agaatatatc tatcagatta
      601 gaatgaaatt ttcaagtttt ggaattaatc caataaaaac tattcgcggg attggttata
      661 aatgggatgt ttaaaaaata ttcaataaaa aatcgtgttt ggcgcgccgt cgttgaaatt
      721 gtctttggag tttgtatcac tgttttaatg attgcgataa tatcgctttc tttttcaaag
      781 ttaaatatag tgacatctca taatgtggga gaagaatttt atattaaaga taaacagtca
      841 atcaaacagt tgaacaatta tatgaagaca t
//
LOCUS       DATXQK010000136          868 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.136, whole genome shotgun sequence.
ACCESSION   DATXQK010000136 DATXQK010000000
VERSION     DATXQK010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..868
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.136"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            82..561
                     /gene="rlmH"
                     /locus_tag="VC394_001811"
     CDS             82..561
                     /gene="rlmH"
                     /locus_tag="VC394_001811"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002991465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEP5995236.1"
                     /translation="MKVKLICVGKLKERYLKDGISEYQKRLSRFCQFEMIELTDERTP
                     DKASFADNQLIMSKEAQRIHKKIGERDFVIALAIEGKQFPSETFSELISGVTVKGYST
                     ITFIIGGSLGLDSIIKKRANMLMSFGLLTLPHQLMRLVLTEQIYRAFMITQGSPYHK"
     gene            complement(719..792)
                     /locus_tag="VC394_001812"
     tRNA            complement(719..792)
                     /locus_tag="VC394_001812"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(756..758),aa:Arg,seq:tct)
BASE COUNT          325 a          119 c          148 g          276 t
ORIGIN      
        1 aaggttttta ttagtagttg aagaaaaata acttgtggat aaattgtgta aaaagtaatg
       61 ttcttgtgat acaattaagc tatgaaagtg aaactaattt gtgttggaaa actaaaagaa
      121 agatacctta aagatggtat ttcagaatat caaaaaagac tttcccgttt ttgtcaattc
      181 gaaatgattg agttaactga tgaaagaaca ccagataaag caagttttgc tgataatcaa
      241 ttgattatga gtaaagaagc tcagcgaatc cataaaaaaa ttggggaacg tgattttgta
      301 attgcattag ctattgaagg aaaacagttt ccttcagaga catttagtga attaatatca
      361 ggggttactg ttaagggata ttcaactatc acgtttatta ttggtgggag tcttggatta
      421 gattcaatta ttaaaaaaag agctaatatg ctaatgagtt ttggattgtt aaccttacct
      481 catcagttaa tgcgtctagt tcttactgag caaatttatc gtgcttttat gattacacaa
      541 ggcagtcctt atcataagta agagagtgtg aactggaata actaactctt accactttat
      601 tagataatag ttcggtataa ttaaaagcaa atattaaaaa aagacgtact aaatcctatc
      661 ttttcaatac actaaggtaa atacaaaaaa aagcactaaa acagtgcttt tttatctttg
      721 atcccagcag gattcgaacc tgcgaccgtt cgcttagaag gcgaatgctc tatccagctg
      781 agctatgaga ccttaaccac atcattttaa caaaaattat ctaaaactgt caatatttat
      841 tttgaaaaaa ctagaaaata acataata
//
LOCUS       DATXQK010000137          867 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.137, whole genome shotgun sequence.
ACCESSION   DATXQK010000137 DATXQK010000000
VERSION     DATXQK010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.137"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..300
                     /locus_tag="VC394_001813"
     CDS             <1..300
                     /locus_tag="VC394_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HEP5995237.1"
                     /translation="NLGSFAIREGKIEGGDAKTVRRVTIDDKTILFDPHPKLERYKSE
                     LVTGTNTFQIHDDMMDTYISLLEGNAIPEDCYINNAIEITKLLINLYSECEKIYL"
     gene            310..>867
                     /locus_tag="VC394_001814"
     CDS             310..>867
                     /locus_tag="VC394_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase"
                     /protein_id="HEP5995238.1"
                     /translation="MDLKITNGFYDPSHLSYEVVERKGLGHPDTLADGIAEQIEIDYS
                     LYCLDKFGVIPHHNFDKIIIRGGHSVQDFGGSDFIEPIKIIFLGRASKKCFNSSIPLF
                     KIQKKAATKYLNRILPNLDVENYVEFETLTSDFTTKTNWFSPEAIEDLPEYLDVPKAN
                     DTATMISYWPLTISEELALMIEGYFY"
BASE COUNT          316 a          108 c          166 g          277 t
ORIGIN      
        1 aatttgggaa gttttgcaat aagagagggc aagattgaag gaggtgatgc taaaacagta
       61 agaagagtta ccattgatga caaaacaata ctatttgatc cacatcctaa actcgagagg
      121 tataagagtg aattggtgac tgggacaaat acgtttcaaa tacacgatga tatgatggac
      181 acgtatatca gtttgttaga aggaaatgct attcctgaag attgctatat caataatgca
      241 attgagataa caaaattatt aattaactta tattcagaat gtgaaaaaat ttatttatga
      301 ggtgtaacta tggatttaaa aattacaaac ggcttttatg acccaagtca tttatcatac
      361 gaagtagtag aacgtaaagg attagggcat cctgatacct tagcagatgg aattgctgag
      421 caaattgaaa ttgattactc actttattgt ttggacaagt ttggtgttat tccacatcat
      481 aattttgata agattattat ccgaggggga cattcagttc aggattttgg tggaagtgac
      541 tttattgaac caattaaaat tatttttcta ggtagggcga gcaaaaaatg ttttaatagt
      601 tcaattccac tttttaaaat tcagaaaaag gctgcaacta aatatttaaa tagaatttta
      661 cctaatttag atgttgaaaa ttatgtagaa tttgagacat tgacctcaga ttttactaca
      721 aaaactaatt ggttctcgcc tgaagcaata gaagatttac cggagtatct agatgtacca
      781 aaggcaaatg atacagctac aatgataagt tattggccat taacaatttc tgaagaatta
      841 gcattaatga ttgaagggta tttttat
//
LOCUS       DATXQK010000138          841 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.138, whole genome shotgun sequence.
ACCESSION   DATXQK010000138 DATXQK010000000
VERSION     DATXQK010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..841
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.138"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..514
                     /locus_tag="VC394_001815"
     CDS             <1..514
                     /locus_tag="VC394_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEP5995239.1"
                     /translation="GYFNSLISYTRMLSEDRSVKLILVEKLLSELHFEVDDLLNINNI
                     EFSICNRLIITSFYGDEENLIRALSNLLVNAIRFMPVLDKKIEVILSESGEQIHFEIW
                     NNGERFSDSTLKKGDKLFYTEDYSRGNKHYGIGLAFVKGVAIKHGGNLQLNNPARGGA
                     SAIISIKKKI"
     gene            671..811
                     /locus_tag="VC394_001816"
     CDS             671..811
                     /locus_tag="VC394_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic streptin"
                     /protein_id="HEP5995240.1"
                     /translation="MNNTIKDFDLDLKTNKKDTATPYVGSRYLCTPGSCWKLVCFTTT
                     VK"
BASE COUNT          302 a          109 c          152 g          278 t
ORIGIN      
        1 aggctatttc aattctttaa tatcttatac acgcatgctt tcagaggata gatcagtgaa
       61 actaatttta gttgagaagc tattaagtga gttacatttt gaagtcgatg acttattgaa
      121 tatcaataat attgaatttt ctatttgtaa tagacttatt atcacttctt tttatggaga
      181 tgaggaaaat ctgataagag ctctttctaa ccttttagtt aatgcaataa gatttatgcc
      241 cgtattagat aaaaaaatcg aagttatact gtctgagtca ggagagcaaa ttcattttga
      301 aatatggaat aatggtgagc gtttcagtga tagtacactg aaaaagggag ataaattatt
      361 ttacacggaa gattatagca gaggtaacaa acattatgga atcggtttgg catttgttaa
      421 aggcgttgct atcaaacacg gtgggaatct tcaattgaat aatccagcta gaggaggtgc
      481 tagcgccata atctcgataa aaaagaaaat ttaatagatt tgattgaaac tggagtattt
      541 caggcaaaaa atcgtaaatt tttgtgattt ttggctaaaa tacttttttt ctgaacaaat
      601 tctgaacttg gggtgtcata ataatcatgt aaatatatcg gatgcaaaaa cgtaagagag
      661 gaactagaaa atgaataaca caattaaaga ctttgatctc gatttgaaaa caaataaaaa
      721 agacactgct acaccttatg ttggtagccg ttacctatgt acccctggtt cttgttggaa
      781 attagtttgc tttacaacaa ctgttaaata ataaagttat cattatcatc aacattataa
      841 t
//
LOCUS       DATXQK010000139          809 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.139, whole genome shotgun sequence.
ACCESSION   DATXQK010000139 DATXQK010000000
VERSION     DATXQK010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..809
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.139"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            62..>809
                     /gene="mf2"
                     /locus_tag="VC394_001817"
     CDS             62..>809
                     /gene="mf2"
                     /locus_tag="VC394_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNase Mf2"
                     /protein_id="HEP5995241.1"
                     /translation="MKLSKQKASLLTAVLLLLSLSITTITVDAAKVRTYPNVSHANTH
                     YKNTASSKLLPFTANYQLQLGELDNLNRATFSHIQLQDRHETKDVRTKINYDPVGWHN
                     YQFPYGDGSKSSWVMNRGHLVGYQFCGLNDEPRNLVTMTAWLNTGAYSGANDSNPEGM
                     LYYENRLDSWLALHPDFWLDYKVTPIYSGNEVVPRQIELQYVGIDSSGELLTIRLNSN
                     KESIDENGVTTVILENSAPNINLDYLNGTAT"
BASE COUNT          272 a          148 c          146 g          243 t
ORIGIN      
        1 tagaaaagtg caatatttga tccactctaa aagctaaaaa tggaaaggtt taggtgttat
       61 catgaaatta tctaaacaaa aggcaagttt gcttaccgct gttttactgt tactttctct
      121 ttctataacg acaataactg ttgacgcagc taaggtacga acttatccga atgtatccca
      181 tgcaaacaca cattataaaa atactgcttc tagtaagctc ttacctttta ctgctaatta
      241 tcagttacaa cttggtgagt tggataacct taaccgcgct accttttcac atatccaact
      301 gcaagataga cacgaaacta aagatgtacg cactaaaata aattatgacc ctgtgggatg
      361 gcataactat caatttccat acggagatgg ctcaaaatct tcatgggtaa tgaatcgtgg
      421 gcatctagtc ggatatcaat tttgtggatt aaacgatgaa ccaagaaatt tagtgacaat
      481 gacagcctgg ctaaacacag gtgcttattc aggagcaaat gacagcaatc ctgaagggat
      541 gttatactat gaaaaccgtt tagattcttg gcttgcactt caccctgact tttggctaga
      601 ttataaagtc actcctatat atagtggcaa tgaagtagtg cctcgacaaa ttgaattaca
      661 gtatgttgga attgattcat ctggtgagtt gcttactata aggctaaata gtaataaaga
      721 aagtattgat gaaaatggtg ttactacagt aatcttagaa aactctgctc caaatatcaa
      781 ccttgactat ttaaatggaa ctgccactc
//
LOCUS       DATXQK010000140          785 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.140, whole genome shotgun sequence.
ACCESSION   DATXQK010000140 DATXQK010000000
VERSION     DATXQK010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.140"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..>785
                     /gene="alp3"
                     /locus_tag="VC394_001818"
     CDS             <1..>785
                     /gene="alp3"
                     /locus_tag="VC394_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_115075552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-like surface protein Alp3"
                     /protein_id="HEP5995242.1"
                     /translation="TALRNRIDEVINNANDPKWSDGSRDEVLTGLEKIKKDIDNNPKT
                     QIDIDNKINEVNEIEKLLVVSLPDKIKYSPEAKHRTVEQHAELDAKDSIANTDELPSN
                     STYNWKNGHKPDTSTSGEKDGIVEVHYPDGTVDDVNVKVTVTSKKTDNTAPTLTVTPE
                     QQTVKVDEDITFTVTVEDENEVELGLDDLKAKYENDIIGARVKIKYLTKEPNKKVMEV
                     TIMKATLADKGAITFTAKDKAGNQAEPKTVTINVLPLKDSNEP"
BASE COUNT          322 a          114 c          152 g          197 t
ORIGIN      
        1 tacagcttta agaaatagga tagatgaagt aataaataat gcaaatgatc ctaagtggag
       61 tgatgggagt cgtgatgaag tcttaactgg attagaaaaa ataaaaaaag atattgataa
      121 taatccaaaa acacaaatag atattgataa taaaattaat gaagtcaatg aaatagagaa
      181 attgttagtt gtatcgctac cagataaaat taagtattcg ccagaggcta agcataggac
      241 tgttgaacaa cacgcggaat tagatgcaaa agatagcatt gcaaatacag atgaattgcc
      301 atcaaattca acgtataact ggaaaaatgg tcataaacca gacacctcaa catcaggtga
      361 aaaagacgga attgttgaag ttcactatcc agatggtact gttgatgatg tgaatgttaa
      421 agtaaccgtt acatcgaaaa aaactgataa tacagctcca acattaaccg tcactccaga
      481 gcaacagact gttaaagtgg atgaagatat tacctttacg gttacagttg aagacgaaaa
      541 tgaagttgaa ctaggtttag atgatcttaa agctaagtat gaaaatgata tcattggagc
      601 tcgtgttaaa attaagtatc ttactaaaga acctaataag aaagtcatgg aagtgacaat
      661 tatgaaagct actttagcag ataagggcgc aattaccttt actgcaaaag ataaagcagg
      721 taatcaagca gaacctaaga cagttaccat caatgttctt ccgcttaagg atagcaacga
      781 accaa
//
LOCUS       DATXQK010000141          774 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.141, whole genome shotgun sequence.
ACCESSION   DATXQK010000141 DATXQK010000000
VERSION     DATXQK010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.141"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..>774
                     /locus_tag="VC394_001819"
     CDS             <1..>774
                     /locus_tag="VC394_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEP5995243.1"
                     /translation="VGLSIGLLLAKEHDVTFFDIDNKKIDLINKRQSPLKEAAINKLL
                     CKAKNINATSSEELAYKDATFIILSLPTNLKFNKLDTSIIEISVSNILKINKKATIVI
                     KSTVPIGFTEYLRNRFHYNDIIFSPEFLREGSTIHDQLYPSRTIVGNESRNSQLFLDI
                     LTDISVEKDSPSLLVGSSEAEAIKLFSNAYLAQKIAFFNELDTFAEMQNLDSKKIIEA
                     MGYDQRIGNSHNNPSFGFGGYCLPKDIKQLEYHFKEIPA"
BASE COUNT          276 a          122 c          126 g          250 t
ORIGIN      
        1 acgttggatt atcgataggg ctcctacttg caaaggaaca cgacgtcacc ttttttgata
       61 ttgataataa aaaaattgat ttaataaata aaaggcaatc ccctcttaaa gaagcagcta
      121 taaacaaact tttatgtaag gcaaaaaata ttaatgcaac ttcttctgaa gaattagcat
      181 ataaggatgc gactttcata atcttgtctt tgccaaccaa cctaaaattt aataagcttg
      241 atacttccat tatcgaaatt tctgtaagta atattttaaa gataaacaaa aaggctacaa
      301 ttgtaataaa gtcgacagtt ccaattggtt ttacagaata tttaaggaat cgatttcact
      361 acaacgatat cattttttca cctgagttcc ttagggaagg atcaactatt catgatcaat
      421 tgtatccttc gagaactata gttggaaatg aatctagaaa ttctcaatta ttcttagaca
      481 tactaacaga tatatcggtt gaaaaagact cgccatcttt attagttggc tcttctgaag
      541 cagaagcgat aaagttattt tcgaatgcat acttggcaca aaaaattgct ttttttaatg
      601 agttggatac gtttgctgaa atgcaaaatt tggactcaaa aaaaattatt gaggctatgg
      661 gatatgacca gagaatagga aattcgcaca ataatccttc tttcggtttt ggtgggtact
      721 gtcttcctaa ggatattaag caattagagt atcattttaa agaaattcca gcac
//
LOCUS       DATXQK010000142          753 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.142, whole genome shotgun sequence.
ACCESSION   DATXQK010000142 DATXQK010000000
VERSION     DATXQK010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.142"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..>753)
                     /locus_tag="VC394_001820"
     CDS             complement(<1..>753)
                     /locus_tag="VC394_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019418808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEP5995244.1"
                     /translation="LYMELLYDFTHDELVHNPIIESTVDTIQSVEFERVSFSYNGKDN
                     VINDISFSIKAGERLAIVGENGSGKSTIFKLACGLYDNYEGNIYINGINLRSIQKKSY
                     YKRISALFQDFLKYELTLRENVGLGELSKLYSDEDLISALKYTGVDSIFYFNQEKLNQ
                     IDLEQQLGNWFEDGRQLSGGQWQKIALSRVYLKEADCYLLDEPSSALDPESETKIFKT
                     FFELSQNKIGIFITHKPSITQSVNKILYLDNG"
BASE COUNT          249 a          152 c           85 g          267 t
ORIGIN      
        1 atccattatc tagatacaaa attttattca cagattgagt aatgcttggc ttatgcgtaa
       61 taaaaatacc aattttattt tgcgataatt caaaaaatgt tttaaaaatt tttgtttcag
      121 actcaggatc taacgcagaa ctaggctcat ctagcaaata gcaatccgct tctttgagat
      181 atactcttga taatgcaatt ttttgccact gtccaccaga taactgtctc ccatcctcaa
      241 accaattccc taattgctgt tccagatcta tctgatttaa tttttcctga ttaaagtaaa
      301 atatcgaatc aactcctgta tacttcagtg cacttattaa atcctcatca ctatataact
      361 ttgataattc acccaatcca acattttcac gcagtgtcaa ttcatacttt aaaaaatctt
      421 ggaacaatgc ggatattctc ttgtagtatg actttttctg tattgaacga agattaatac
      481 catttatata tatattgcct tcataattat cgtaaagacc gcaggctaat ttgaaaatcg
      541 tactttttcc agaaccattt tcaccaacaa ttgctaacct ctctcctgct ttaattgaaa
      601 aacttatatc gtttattaca ttatccttac cattatatga aaaagaaact ctttcaaatt
      661 caactgactg aattgtatca accgtacttt cgattatagg gttatgtact aactcatcat
      721 gcgtaaaatc atacaataac tccatgtaca atg
//
LOCUS       DATXQK010000143          749 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.143, whole genome shotgun sequence.
ACCESSION   DATXQK010000143 DATXQK010000000
VERSION     DATXQK010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.143"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(142..681)
                     /locus_tag="VC394_001821"
     CDS             complement(142..681)
                     /locus_tag="VC394_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEP5995245.1"
                     /translation="MLQTGFRFGEAMAITWDDIDFERNSIYTYRRYSSIKKDFTKPKT
                     RTSIRRVPVSHDLKELLLELKFQQERMLRGLHIKNTDQLIFYDYRFGMISNNAVNKFL
                     ASSLEKLEIESKMSSTGARHTYGSYLLANGVDIWAVAKLMGHKDIKQLIETYGHLLLE
                     VEEKENDQLLKILNNKGSF"
BASE COUNT          234 a          127 c           96 g          292 t
ORIGIN      
        1 tgtagttaaa tggtataata aagcaatggt aatgcttcgt tccgagttcg attctcggtg
       61 gtggcagaaa atatacgagt gaaaccttat tataataggg tttcttttat ttttgttctt
      121 aatttgttct tgatagtcat tttaaaaact acctttatta ttaagaattt ttaaaagctg
      181 atcattttcc ttttcttcca cctccagaag caaatgacca tatgtttcta ttaattgttt
      241 gatatcttta tgccccatta attttgcaac cgcccaaata tctactccgt tagccaacaa
      301 ataacttcca taagtatgtc tagcacctgt tgatgacatc ttagattcta tttctaattt
      361 ttccaatgag cttgccaaga atttgttaac agcattattt gatatcatac caaacctata
      421 gtcataaaaa atcaattgat ctgtattttt tatatgcaaa cctctcagca ttctttcttg
      481 ttgaaatttt agttcaagca ataactcttt taaatcatga cttactggaa ctctccttat
      541 tgaagtcctt gttttaggct ttgtaaagtc cttttttatt gaagaatatc ttcgatatgt
      601 ataaatacta tttctttcaa aatcaatatc atcccaagtt attgccattg cttcaccaaa
      661 cctaaaacca gtttgtaaca ttgtgaaaag aaaaaaaggg attattgagt ctgaataatt
      721 agctattctt tttaaatatg ctaataatt
//
LOCUS       DATXQK010000144          747 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.144, whole genome shotgun sequence.
ACCESSION   DATXQK010000144 DATXQK010000000
VERSION     DATXQK010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..747
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.144"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..>747
                     /locus_tag="VC394_001822"
     CDS             <1..>747
                     /locus_tag="VC394_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEP5995246.1"
                     /translation="DYMKNTNFAEDFFKKLNNDGITDPEVNKEMIYINKSGANIQNKV
                     NGTDYQLLDNKVATIYIPDKFKEKKKSIENTVVAEQFTGTKYTKENLAVQIIPNEKKI
                     FYFNENGNEHDENKEASPMANVTDSKGNIVVVLDTDKMVASKDFSLASNIVNSSLFSP
                     EAIKKINDLSLHLNFSINPVDVYQLVKLNIQSLKHQIFLSKVLQKIIYGIVFLLIYQY
                     AQLFISSKQNDYVKKIILGLSKTRIAISSL"
BASE COUNT          305 a          102 c          111 g          229 t
ORIGIN      
        1 agattacatg aaaaatacta actttgcaga agattttttc aaaaaattaa ataatgatgg
       61 tataacggat ccagaagtaa ataaagagat gatttatatc aataaatcgg gcgctaatat
      121 tcaaaataaa gtcaatggaa cagactatca gctgttagat aataaggtag caacaatcta
      181 tattcctgat aaatttaaag agaaaaagaa atcaattgaa aataccgttg tggcagaaca
      241 atttactgga actaagtata caaaagaaaa ccttgcagtg cagataattc ctaatgagaa
      301 aaaaattttt tattttaatg agaatggaaa cgagcatgat gaaaataaag aagcctcacc
      361 tatggcaaat gtaacagata gtaaaggtaa tatagtcgtt gtgttagata ctgataaaat
      421 ggtagcaagc aaagattttt ctttggctag caatatagta aatagctctc tttttagtcc
      481 tgaagcaata aaaaaaatta atgacctgag tctacattta aatttttcaa ttaatccagt
      541 agatgtttac caacttgtaa aacttaatat tcaatcctta aagcaccaaa ttttcctttc
      601 gaaagttttg cagaaaatta tttacggcat tgtatttcta ctaatttatc aatatgctca
      661 attatttatt tcttcaaaac agaatgacta tgtgaaaaaa ataattttag gcctctcaaa
      721 aacacgcata gcaatttcga gtctcaa
//
LOCUS       DATXQK010000145          734 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.145, whole genome shotgun sequence.
ACCESSION   DATXQK010000145 DATXQK010000000
VERSION     DATXQK010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.145"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..>734
                     /locus_tag="VC394_001823"
     CDS             <1..>734
                     /locus_tag="VC394_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5995247.1"
                     /translation="TILHLYPLLVIVLYYRSFRQQISVVRRSLILFSLLLLVILGSEL
                     YGEMLDVNQAFNNLGWYLFPLIMSVIYYFKTIHDKLSFVTQRWLGDYKARIELLFLLL
                     ILCWIVLMKVLVKDFLLFFIVVDASTLFCLVVISCIFYYLKDSKQNFDYENRRLNYFM
                     KSEENMRVEFSNYLHDDVLQNIIAIKNLLSLENSNITHGFIVNELNDLVSGIRDEMDT
                     YYPIVPANQTMKENIQSLFDDIVKSR"
BASE COUNT          237 a           95 c          122 g          280 t
ORIGIN      
        1 ttactatttt acacctgtat ccgttactag ttatagtgct atattatagg tcctttaggc
       61 aacaaatatc agttgttaga cgttcattga tactattttc tttacttttg ctggtaatac
      121 ttggtagtga actttatgga gaaatgctag atgttaatca agcctttaat aatttgggat
      181 ggtatctgtt tccccttata atgagtgtaa tatactattt taagaccata catgataaat
      241 tgagttttgt tacccaaaga tggttaggtg attacaaagc tcgtatagaa ctattatttc
      301 tacttcttat cctttgttgg attgtactca tgaaggtatt agttaaagat tttttgctat
      361 tttttatagt tgttgacgca agcacattgt tttgtctagt ggtaatttct tgcatctttt
      421 attatttaaa agactccaaa caaaattttg actatgaaaa tagaagactg aactatttta
      481 tgaaatcaga ggaaaacatg agagtggagt tctcaaatta tttgcatgat gatgttttac
      541 aaaatatcat agcgataaaa aacttgcttt ctctagaaaa tagtaatata acacatggtt
      601 ttattgttaa tgagttgaat gatttagttt ctggtatcag agatgaaatg gatacttact
      661 acccaatagt tcccgctaat caaacaatga aagaaaacat acagtctctt tttgatgata
      721 ttgttaaaag taga
//
LOCUS       DATXQK010000146          731 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.146, whole genome shotgun sequence.
ACCESSION   DATXQK010000146 DATXQK010000000
VERSION     DATXQK010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.146"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..657
                     /locus_tag="VC394_001824"
     CDS             <1..657
                     /locus_tag="VC394_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HEP5995248.1"
                     /translation="CSEDREAEFDLGIAHGITGPMLILAKLKSEKILSVEVGDILNKA
                     INLMFLFRRDDKLWPGKIYTSNILDLDLNYLPTRMAWCYGTPGTAVALFKMCQLIDLN
                     FIDDINESLIAQMRSSDKNVYSLTMCHGLAGVTFIYDLIGKNYSSERLTRFAEELRQQ
                     IITSFSTTKVFGYTDREKFGEIEVELESIGILQGVSGIVLFLLNPYSDEELLWQRIFI
                     "
BASE COUNT          257 a           88 c          148 g          238 t
ORIGIN      
        1 tgttctgaag atagagaagc tgaatttgat ctaggaattg cacatggaat tactggtcct
       61 atgctaatcc tagcaaaatt aaaatcagaa aaaattttga gtgtagaagt aggagatata
      121 ctgaataaag ctataaattt aatgttttta tttagaaggg atgacaaact atggcctgga
      181 aaaatttata ctagtaatat tttggatctt gatttaaatt atcttcctac tagaatggca
      241 tggtgttacg gtacgcctgg aactgcagtt gcccttttta aaatgtgtca attgatagat
      301 ttaaatttta ttgacgatat taatgaaagt ttaattgctc agatgagaag ttcagacaaa
      361 aatgtctatt ctcttacaat gtgccatggt ttggcaggag taacttttat atatgacttg
      421 attggtaaaa attattcaag tgagaggcta actcgctttg cagaagagct tcgccaacaa
      481 ataattacat cgttttcaac tacaaaagta tttgggtata ctgacagaga aaaatttgga
      541 gaaatagaag ttgaactgga gtcaataggg atattgcaag gggtatcagg gatagtgtta
      601 ttcttattaa atccttacag tgacgaggag ctattgtggc aaagaatatt tatttaattt
      661 gaaaaggaga acaaaatgta taaggtattt ccaactggta tgattcgtat tccattgcaa
      721 aaacaagaat t
//
LOCUS       DATXQK010000147          707 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.147, whole genome shotgun sequence.
ACCESSION   DATXQK010000147 DATXQK010000000
VERSION     DATXQK010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.147"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..496)
                     /locus_tag="VC394_001825"
     CDS             complement(<1..496)
                     /locus_tag="VC394_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEP5995249.1"
                     /translation="MINPSKQNQTIFLWNMLGSLSTAVISVILLMVVTRLLTSADSDI
                     YAFAYSFANMMVVVGLFQVRNYQATDINEKYSFSQYLVARLMTCLLMLAITVIYLTLT
                     KTDSYKSTIVFLVCFYRSTDAFSDLYQGMFQQHERLDIAGKSLAYRNTLIFMVYTAII
                     LYSKN"
     gene            complement(489..>707)
                     /locus_tag="VC394_001826"
     CDS             complement(489..>707)
                     /locus_tag="VC394_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HEP5995250.1"
                     /translation="LLILLFTWLPNILNKMSHFLGIHSPTNMLFFLGFCLSLAIIFSL
                     TNNISLQNDKVKRLTQEVALMKKEKTND"
BASE COUNT          259 a          109 c          110 g          229 t
ORIGIN      
        1 gatttttgga atataatatt attgctgtgt acaccataaa aataagagta tttctgtagg
       61 ctagcgattt acctgcaata tccagccgtt catgttgttg gaacattccc tgatataaat
      121 ccgaaaaagc atctgtcgat ctatagaaac agactagaaa gacaattgta ctcttgtagc
      181 tatctgtctt agttaacgtt aaataaatta ctgttatggc taacattaat aaacaagtca
      241 tcagtcttgc cactaagtac tgactaaagg aatatttttc attaatatct gttgcttgat
      301 aattacgaac ctgaaaaagt ccaacaacta ccatcatatt agcaaaagaa taggcaaagg
      361 cgtaaatatc tgaatcagcc gaagttagta acctagtaac caccataagt aatataactg
      421 atattacagc tgttgataaa cttcctaaca tattccataa aaaaatcgtt tggttttgtt
      481 ttgaaggatt aatcattggt tttctctttt ttcataaggg ccacttcttg ggtcagacgt
      541 tttactttat cattttgaag tgaaatatta ttagtcagtg aaaaaataat agctaaactc
      601 aaacaaaaac ctaaaaagaa taacatattg gttggtgaat gaatacccag gaagtgagac
      661 attttattta atatgttagg taaccacgta aatagtaaaa ttaataa
//
LOCUS       DATXQK010000148          698 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.148, whole genome shotgun sequence.
ACCESSION   DATXQK010000148 DATXQK010000000
VERSION     DATXQK010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.148"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(218..631)
                     /locus_tag="VC394_001827"
     CDS             complement(218..631)
                     /locus_tag="VC394_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEP5995251.1"
                     /translation="MKLNAIHHVAIIVSDYHLSKDFYVNKLGFEIIRENYRSDKHDYK
                     LDLSCGRIELEIFGKVTSDPNYQAPPKRVSQPEFKSEACGLRHLAFRVTNIESYVDDL
                     KSLGIPVEPIRHDDYTGEKMTFFFDPDGLPLELHE"
BASE COUNT          248 a          131 c           94 g          225 t
ORIGIN      
        1 attataacac tgtaagagtt tatcgtcaaa tattttgctt ttcctatagt tattctatta
       61 aaaaacttcg acaagattac tccaaaaatt gctcttcact ttgaaagtta accaaatcat
      121 ttaacgatca aaagagatta atcaagcaaa agaagataca acaaataact atttttaacg
      181 agtttaatag cacgatccag tcgttaacct aataagtcta ttcatgtaat tctaaaggca
      241 aaccatccgg atcaaaaaag aaagtcattt tctcacctgt gtaatcatca tgcctaatag
      301 gctccactgg aattcctaag ctctttagat catctacata actctcaatg ttggtaacac
      361 gaaaagcaag gtgcctcaaa ccacaggcct cacttttaaa ttctggttga cttacacgtt
      421 taggaggagc ttgatagttg gggtcacttg tcaccttacc aaaaatctcc aattcaatac
      481 gaccacaaga taaatctaat ttgtaatcat gcttgtcaga acgataattt tctctgataa
      541 tttcaaatcc gagtttattc acataaaaat cttttgacag atgataatca gatacaataa
      601 tagcaacgtg atgaatggca tttagtttca tcatatttct cctttcaaaa aaagtaggat
      661 gagataaatt ttctctatgg tcctactttt taaatatt
//
LOCUS       DATXQK010000149          659 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.149, whole genome shotgun sequence.
ACCESSION   DATXQK010000149 DATXQK010000000
VERSION     DATXQK010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.149"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(102..635)
                     /locus_tag="VC394_001828"
     CDS             complement(102..635)
                     /locus_tag="VC394_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEP5995252.1"
                     /translation="MVNRKENTKQAILKAMVMLLKTESFDDITTVKLSKRAGISRSSF
                     YTHYKDKYEMIDYYQQTFFHKLEYIFEKKYQNKEQAFLEVFEFLQREQLLSSLLSANG
                     TKEIQAFIINKVRLLITTDLQDKFSTEELSQTEKEYQSIYLAHAFFGVCQSWIAKGKK
                     ESPQEMTQFVLKMLTST"
BASE COUNT          195 a          104 c          111 g          249 t
ORIGIN      
        1 ataacaaatt tatcctgatc ccttcatttt actatggctt ctaaaaaagt tatcactaag
       61 gctgatgact tgtaaaatga tactttcgta agggttatta tttaggtact cgtaagcatt
      121 ttgagaacga attgtgtcat ttcttgaggt gattcttttt tacctttagc aatccaactt
      181 tgacagacac caaaaaaagc atgggctaag tagatacttt ggtattcttt ttccgtttga
      241 gataactctt cggtactgaa cttgtcctgt aaatcagtag tgatgagtag gcgaacttta
      301 ttgatgataa aggcctgtat ttctttagta ccgttagcag acaaaaggga agagagaagc
      361 tgttctcttt ggagaaattc aaatacttca aggaaggctt gttctttatt ttgatatttt
      421 ttctcaaaaa tatattctaa tttgtgaaaa aaggtttgct gataatagtc aatcatttca
      481 tatttatctt tatagtgtgt gtaaaaacta gagcgactaa ttccagctct tttagataat
      541 ttaactgtgg taatatcgtc aaaactttcg gtttttagta acataaccat tgcttttaaa
      601 attgcttgct tcgtattttc tttacggttg accatctacc atctctcctt ttgaacact
//
LOCUS       DATXQK010000150          644 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.150, whole genome shotgun sequence.
ACCESSION   DATXQK010000150 DATXQK010000000
VERSION     DATXQK010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.150"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            89..>644
                     /locus_tag="VC394_001829"
     CDS             89..>644
                     /locus_tag="VC394_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5995253.1"
                     /translation="MIESRLKFDKITSFDEFNKYYWYREELSQICKLLGLEYRGTKQE
                     LTYIIEQYFKGDVIKKSSIKNIKKQVDYITLDMPLLECGFSFNSKFREYFSVVTGVLP
                     FKFTADMATAWRKVKRQNDLSFTIQDMLKVYYGKSDYAKYDNSVCQWNQFLKDFCADE
                     NSCNYSNKIQVAAILWKEVRDSKNK"
BASE COUNT          250 a           69 c          113 g          212 t
ORIGIN      
        1 agtcatgttc aagtgttatg aaaataatga aatagataaa ttggagtagc ctattcatat
       61 atttagatct tttcgaaagg ataaaacaat gatagaaagt agacttaagt ttgataaaat
      121 cacatcgttt gatgagttta ataaatacta ttggtatcgt gaagaacttt cacagatatg
      181 caagttatta ggattagaat atagaggtac aaaacaagaa ctcacttata ttattgagca
      241 gtactttaag ggggatgtga ttaaaaagtc atcaataaaa aacataaaga aacaagttga
      301 ttatattact ttagatatgc ctttacttga gtgtggattt tcctttaatt caaaattcag
      361 agaatatttt tctgttgtaa caggtgtttt gccttttaaa tttactgcag atatggcaac
      421 ggcttggaga aaagtaaaaa gacaaaatga tttgagtttt acaattcaag atatgctcaa
      481 agtttattat ggaaaatcag attatgctaa gtatgataat tctgtttgtc aatggaatca
      541 atttttaaag gatttttgtg cagacgaaaa tagctgtaac tactctaata aaatacaagt
      601 agctgcaatt ctttggaaag aagtcagaga ttcaaaaaac aaaa
//
LOCUS       DATXQK010000151          636 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.151, whole genome shotgun sequence.
ACCESSION   DATXQK010000151 DATXQK010000000
VERSION     DATXQK010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.151"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..>636)
                     /locus_tag="VC394_001830"
     CDS             complement(<1..>636)
                     /locus_tag="VC394_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HEP5995254.1"
                     /translation="ESYIHDLVTNDFLISDLRPPICNINSLDYLLSKLEEGTLCTDLT
                     TLKKMIEDYNDLNIGEGRDLLLQIFDKMESIYKYDNGGNYLKVDSLAEYEGGFNILSE
                     TARESIERLAEMFVKLNTNVKRRGGRKNSLEEYQLKFIGKYGENCSIPFVEVINKDAG
                     IGFPEYYKGGEGEAIELSDPIMQMFEKKYEEALLNGGHIEFYSKDLDDFQT"
BASE COUNT          193 a          128 c           68 g          247 t
ORIGIN      
        1 ctgtttgaaa atcatctaaa tcttttgaat aaaattcgat atgccctcca tttaatagag
       61 cttcctcata tttcttttca aacatttgca ttattggatc gcttaactct attgcttcac
      121 cttctcctcc tttgtaatat tcaggaaaac ctatgcctgc atctttattt attacttcaa
      181 caaaaggaat tgagcagttt tctccatatt tgccaataaa tttcaactga tactcttcaa
      241 gtgaattttt cctcccccct cttctcttta catttgtatt taattttaca aacatctctg
      301 cgagtcgttc tatactttcc cgagccgttt cagatagaat attaaaaccg ccttcatact
      361 ctgctaatga atcaactttt aagtaatttc caccgttatc atatttataa atactttcca
      421 ttttatcaaa aatctgcaaa agtaagtctc taccttcccc aatatttaaa tcgttgtaat
      481 cttcaatcat cttctttaaa gtagttaagt cagtacatag tgttccttct tctaatttag
      541 atagtagata atctaaggaa ttgatattac aaattggtgg tcttaaatct gaaattaaaa
      601 aatcatttgt aactaagtcg tgtatatatg actcta
//
LOCUS       DATXQK010000152          633 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.152, whole genome shotgun sequence.
ACCESSION   DATXQK010000152 DATXQK010000000
VERSION     DATXQK010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.152"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(5..78)
                     /locus_tag="VC394_001831"
     tRNA            complement(5..78)
                     /locus_tag="VC394_001831"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42..44),aa:Arg,seq:acg)
     gene            complement(95..179)
                     /locus_tag="VC394_001832"
     tRNA            complement(95..179)
                     /locus_tag="VC394_001832"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(143..145),aa:Leu,seq:taa)
     gene            complement(188..259)
                     /locus_tag="VC394_001833"
     tRNA            complement(188..259)
                     /locus_tag="VC394_001833"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(225..227),aa:Gly,seq:gcc)
     gene            complement(272..344)
                     /locus_tag="VC394_001834"
     tRNA            complement(272..344)
                     /locus_tag="VC394_001834"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(309..311),aa:Thr,seq:tgt)
     gene            complement(355..436)
                     /locus_tag="VC394_001835"
     tRNA            complement(355..436)
                     /locus_tag="VC394_001835"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(400..402),aa:Leu,seq:tag)
     gene            complement(442..514)
                     /locus_tag="VC394_001836"
     tRNA            complement(442..514)
                     /locus_tag="VC394_001836"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(479..481),aa:Lys,seq:ttt)
     gene            complement(546..618)
                     /locus_tag="VC394_001837"
     tRNA            complement(546..618)
                     /locus_tag="VC394_001837"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(583..585),aa:Asp,seq:gtc)
BASE COUNT          165 a          185 c          136 g          147 t
ORIGIN      
        1 ctgatgcacc ctagaggagt cgaacctcta accgcctgat tcgtagtcag gtactctatc
       61 cagttgagct aagggtgcta tttcaaaaaa ttaatgccga ggaccggaat cgaaccggta
      121 cgatgtaaac catcgcagga ttttaagtcc tgtgcgtctg ccagttccgc caccccggct
      181 cctcaaaagc gaacgacggg attcgaaccc gcgaccccca ccttggcaag gtgatgttct
      241 accactgaac tacgttcgca actaatctca atgccggcta catgacttga acacgcgacc
      301 ctctgattac aaatcagatg ctctaccaac tgagctaagc cggctaatct tttatgcggg
      361 ttaagggact tgaaccccca cgccgttaag cgccagatcc taaatctggt gcgtctgcca
      421 attccgccaa acccgctttt atgacccgta ctgggctcga accagtgacc cattgattaa
      481 aagtcaattg ctctaccaac tgagctaacg agtctttcct tcaaaaaata tgactactct
      541 ttgaacggtc ccgacgggaa tcgaacccgc gatcttcgcc gtgacagggc gacgtgataa
      601 ccgctacact acgggacctg tattttccaa tac
//
LOCUS       DATXQK010000153          627 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.153, whole genome shotgun sequence.
ACCESSION   DATXQK010000153 DATXQK010000000
VERSION     DATXQK010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.153"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            11..>627
                     /locus_tag="VC394_001838"
     CDS             11..>627
                     /locus_tag="VC394_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HEP5995255.1"
                     /translation="MAEKNSNAQLARQFIIGLPKELSLSENKNLVERFIKENLTSQGM
                     IVDYAIHDESQDKNGNIHCHIMTIMRPINEKGEFLAKSKKEYILDEKGEKILNKNGKP
                     KTRKVELTIWNDKGNVEKWRENFSDLCNEYLAKNKIEKRVDHRSFKRQNSDYLPTIHL
                     GSAASAMERKGIETDKGNYNREIRKYNDLVKTIKEEIKTLKGWIG"
BASE COUNT          312 a           74 c          112 g          129 t
ORIGIN      
        1 taaagtagaa atggcagaaa aaaattctaa tgcacaacta gcaagacaat tcataatagg
       61 actaccaaaa gaattatctt taagtgaaaa taaaaaccta gtcgaaagat ttataaaaga
      121 aaatctaaca tcacaaggaa tgatagtaga ttatgcaatc catgatgaga gtcaagacaa
      181 aaatggaaat atccattgtc atataatgac cataatgcga ccaataaacg aaaaaggaga
      241 gttcttagca aagtcaaaaa aagaatatat cctagatgaa aaaggagaaa agattttaaa
      301 caaaaatgga aaacctaaaa caagaaaagt agaactaaca atctggaatg ataagggcaa
      361 tgtagaaaaa tggagagaaa atttttcaga cctttgcaat gaatatctag caaaaaacaa
      421 gatagaaaaa agagtagacc ataggagttt taaaagacaa aattctgatt atttaccgac
      481 aatccattta ggatcagcag caagtgcaat ggaaagaaag ggaatagaaa ctgacaaagg
      541 aaactataat agagaaataa gaaaatataa cgaccttgta aagacaataa aagaagagat
      601 aaaaacatta aagggttgga taggaaa
//
LOCUS       DATXQK010000154          617 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.154, whole genome shotgun sequence.
ACCESSION   DATXQK010000154 DATXQK010000000
VERSION     DATXQK010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.154"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..>617
                     /locus_tag="VC394_001839"
     CDS             <1..>617
                     /locus_tag="VC394_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5995256.1"
                     /translation="MEEMFDNFRKKQYSNIPTLEINDLMYYINAMQKVTSEEELNGTN
                     LFYWLVTISRVDTESPIILANLEKNIDVRKREIEHGDNEGLVVDTRLLFDPFRQILVV
                     YNQRGTINNYDLRRFFCQIIGVRGLKFDIILNSDAFKRVGKLDVVNSISYTVASPTNF
                     KEFRDDTQSENADLKFANSMLGESMQVVIKSNHLSKKNIFDKFSD"
BASE COUNT          234 a           63 c          118 g          202 t
ORIGIN      
        1 agatggagga aatgtttgat aattttagaa aaaaacaata tagtaacatt ccaacattag
       61 agataaatga tcttatgtat tatataaatg caatgcagaa ggtaacaagt gaagaagaat
      121 taaatggaac gaatctattt tattggttag ttaccatttc acgggtggat acagaatctc
      181 caattattct tgcgaatctt gaaaaaaata ttgatgtaag aaaaagggaa attgaacatg
      241 gggataatga gggccttgtg gttgatacaa gattattatt tgacccattt agacaaattt
      301 tagttgttta taatcaaaga ggtacaatta ataattacga cttaagaagg tttttttgtc
      361 aaataattgg agttagagga ttgaagtttg atattatatt aaacagtgat gctttcaaaa
      421 gggttggtaa gcttgatgtt gtaaattcaa ttagttatac tgttgcaagc ccaacaaact
      481 ttaaggagtt tcgagatgat actcaaagtg aaaatgccga tttaaagttt gcaaattcta
      541 tgctaggaga gtcaatgcag gttgttataa agtcaaacca tctttctaaa aaaaatatat
      601 ttgataaatt ttcagat
//
LOCUS       DATXQK010000155          612 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.155, whole genome shotgun sequence.
ACCESSION   DATXQK010000155 DATXQK010000000
VERSION     DATXQK010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.155"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            251..>612
                     /locus_tag="VC394_001840"
     CDS             251..>612
                     /locus_tag="VC394_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5995257.1"
                     /translation="MRNILRFLGIVIISAVILFSIGSFYDLPLMKNIQLICWSFILDL
                     FVFVYKQRQTTEVLTWYQVVKQFWLFIKCTILIPTLIAFIIIIGCLTSISEVSIYFYL
                     HLTVVCYTIGMILSLGRL"
BASE COUNT          208 a           71 c          102 g          231 t
ORIGIN      
        1 agatacttgg tgtggaaaaa aataccagaa attaagatta attctactga gctatcatgc
       61 aatcacattt aaggggaaaa tggattactt atacaatatc agtacttatg atatttaact
      121 ttagagacta aagagttaaa agcaaaatac gtattgaaaa ttggttaaaa aagttataac
      181 tgtctctgat aacaggaata ataatttgtt cggttatatt tgctaccgat agagtagagg
      241 aggtgaagat atgcgtaata tattaagatt tttaggtatt gttattattt ctgcagtgat
      301 tctatttagt ataggtagtt tctacgattt accattaatg aaaaatatac aacttatctg
      361 ttggagtttt atccttgatt tattcgtttt tgtctacaaa caaagacaaa cgacagaagt
      421 gctcacatgg tatcaagttg ttaagcaatt ttggttattt attaagtgta caattcttat
      481 tcctacctta attgctttta ttataataat aggatgtttg acgagtatat cagaggtatc
      541 aatttacttt tatttacacc ttactgttgt ttgctataca ataggaatga ttttatcatt
      601 aggtcgtctt at
//
LOCUS       DATXQK010000156          605 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.156, whole genome shotgun sequence.
ACCESSION   DATXQK010000156 DATXQK010000000
VERSION     DATXQK010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.156"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            20..>605
                     /locus_tag="VC394_001841"
     CDS             20..>605
                     /locus_tag="VC394_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011106780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEP5995258.1"
                     /translation="MKKYLKKIGKMMTKMYYDENPDSLHDIHEVKVELLNHPFTFLTD
                     SGVFSKKMVDFGSQVLLETLNFKENERVLDLGCGYGPLGISLAKVQRVDATLVDINNR
                     ALDLARKNATNNQVAVTIFQSNIYENISGHFEHIISNPPIRAGKRVVHSIIEKSIDFL
                     VVNGDLTIVIQKKQGAPSAKAKMATIFGNVEILRK"
BASE COUNT          219 a           80 c          123 g          183 t
ORIGIN      
        1 catttcaaag tatgatagaa tgaaaaagta ccttaaaaag ataggaaaaa tgatgacaaa
       61 gatgtattat gatgaaaatc cagatagtct acacgatatt catgaagtaa aagtagagct
      121 gcttaatcat ccctttactt ttttgacgga ttcaggtgtc ttttcaaaga aaatggttga
      181 tttcggtagt caagtattat tagagacact aaatttcaag gagaacgaac gtgtactgga
      241 cttaggttgc ggctatggtc ctcttgggat tagtctagcc aaggttcaaa gggtagacgc
      301 aaccttagtg gacatcaata atcgtgctct tgatttagct agaaaaaatg caactaataa
      361 ccaagtggca gtaactattt ttcaatcaaa tatttatgaa aatattagtg gccattttga
      421 gcatattatt agtaatcctc ctattagagc aggaaagaga gtagttcata gtattattga
      481 aaaaagcata gattttttag tggttaatgg tgacttaacg attgtgattc aaaaaaaaca
      541 aggagctcct agcgctaaag caaaaatggc aactattttt ggtaatgttg agatattaag
      601 gaaag
//
LOCUS       DATXQK010000157          601 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.157, whole genome shotgun sequence.
ACCESSION   DATXQK010000157 DATXQK010000000
VERSION     DATXQK010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.157"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..144
                     /locus_tag="VC394_001842"
     CDS             <1..144
                     /locus_tag="VC394_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HEP5995259.1"
                     /translation="KNRKHEKINLYDAKPNSTRSDVLKKYKDNKTINMKDFSHFDIYL
                     WTK"
     gene            342..557
                     /locus_tag="VC394_001843"
                     /pseudo
     CDS             342..557
                     /locus_tag="VC394_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          245 a           97 c           91 g          168 t
ORIGIN      
        1 aaaaatagga aacatgaaaa aatcaattta tatgacgcaa aacccaatag caccagatct
       61 gatgtcttaa aaaaatataa agacaataag actataaaca tgaaagattt cagccatttt
      121 gatatttatc tttggactaa ataagctaaa agctctggat aaatatgatc aattgaccta
      181 taataacatt gataccaaat ctaaaactgc tcagattgaa ttgcgcccca aaagttagac
      241 aaaaaatcta ataattgggg tgtttttgtc tggcattaag tttagctaat aaccttgctt
      301 aaatcacatt aaaccaataa tggaaaaagg catctccaaa taaatgctat gacggaacta
      361 tatgatagag tgatcaaggt tggcaatatc aacacaacta ttatcatcat ttcctaaact
      421 ctaaaggaat tcgtccgtct atgtcacgta aaagaaacag gcctgacaat ggcatgatgg
      481 aatctttctt tggcatctta aaatcagaaa tgttctatgg ttatgagaat aactgtaaat
      541 cactagaaga ctcagaataa gcgattacaa atgacattga ttattacaac gataaacgaa
      601 t
//
LOCUS       DATXQK010000158          554 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.158, whole genome shotgun sequence.
ACCESSION   DATXQK010000158 DATXQK010000000
VERSION     DATXQK010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.158"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
BASE COUNT          183 a          116 c           68 g          187 t
ORIGIN      
        1 tatttctatt tttatttttt aacaaaaaag tcgacttatg aaagaagtcg acatagaaaa
       61 aaatattctc actatatttc aatttaaatt gcatgctcgt atcttcgcac actcctttca
      121 ctatgtttct caacatgagc tactcagtcg aataagtcca ccttatcctt tagctagaca
      181 aagtctaaca caccgcccac aataccatac ctaatccgcc tcaccacgtt ttcagtaatc
      241 attgtcaaca ccccttgcag agtagcagtt ttagtttttt gctcaacaca ggctaactaa
      301 cataaacctt agtgtgcttt tgaggtctga actttacttg tttagttgaa cacacagtaa
      361 ttactataat catggccaca actattttgc atcaattttt atagtaaaac taagctaggt
      421 aggaataact atctaacttt ttttgcttaa acaagcaaaa ttcaaacttc aaaagttatc
      481 agacaacttg gtatagtaaa ctacacttgc tttacttctg aagaatttca cgctataata
      541 aaagtgtcta tgtt
//
LOCUS       DATXQK010000159          548 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.159, whole genome shotgun sequence.
ACCESSION   DATXQK010000159 DATXQK010000000
VERSION     DATXQK010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.159"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..>548
                     /locus_tag="VC394_001844"
     CDS             <1..>548
                     /locus_tag="VC394_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HEP5995260.1"
                     /translation="KKTKLIFSFTSIFIAIISRPVFGLEVDNNSLLRNIYSTIVYEYS
                     DTVIDFKTSHNLVTKKLDVRDARDFFINSEMDEYAANDFKAGDRIAVFSVPFDWNYLS
                     KGKVTAYTYGGITPYQKTSIPKNIPVNLWINRKQISVPYNEISTNKTTVTAQEIDLKV
                     RKFLISQHQLYSSGSSYKSGKL"
BASE COUNT          205 a           87 c           79 g          177 t
ORIGIN      
        1 tgaaaaaaac aaaacttatt ttttctttta cttcaatatt cattgcaata atttctcgtc
       61 ctgtgtttgg attagaagta gataataatt cccttctaag gaatatctat agtacgattg
      121 tatatgaata ttcagataca gtaattgatt ttaaaaccag tcataactta gtgactaaga
      181 aacttgatgt tagagatgct agagattttt ttattaactc cgaaatggac gaatatgcag
      241 ccaatgattt taaagctgga gatagaatag ctgtgttctc cgtcccattt gattggaact
      301 atttatcaaa aggaaaagtc acagcatata cgtacggcgg aataacaccc taccaaaaaa
      361 cttcaatacc taaaaatatc cctgttaatt tatggattaa tagaaagcag atctctgttc
      421 cttacaacga aatatcaact aacaaaacaa cagttacagc tcaagaaatt gatctaaagg
      481 ttagaaaatt tttaatatca caacatcaat tatattcttc tggttctagc tacaaaagtg
      541 gtaaatta
//
LOCUS       DATXQK010000160          514 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.160, whole genome shotgun sequence.
ACCESSION   DATXQK010000160 DATXQK010000000
VERSION     DATXQK010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.160"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..>514)
                     /locus_tag="VC394_001845"
     CDS             complement(<1..>514)
                     /locus_tag="VC394_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HEP5995261.1"
                     /translation="LGANIGSGQAGRSFGRFYGLSETVRETIKNLNHQNNTNVELSFV
                     PKQIRLANVIQNYSDESYNTSFFTTSWDSENELRLEDIYIRYSDGKFHFTTIDGKNEL
                     KFTMNNMLNSDSQSRVLRLLVDLSEFEYGLSHWSLFPWDILAQERVYIPEILFEDITI
                     TTAQWNLSAIR"
BASE COUNT          171 a          105 c           64 g          174 t
ORIGIN      
        1 tctaattgct gataaattcc actgagcagt ggtaatcgta atgtcttcaa ataaaatttc
       61 aggaatatat actctttcct gagccaaaat atcccaagga aataatgacc aatgcgataa
      121 accgtactca aactcagata aatcaaccaa caatctcaaa accctgcttt ggctatcgga
      181 atttagcata ttattcatgg taaatttcaa ctcatttttc ccatcgatag ttgtaaaatg
      241 aaacttacca tcactatatc ttatataaat atcctccaat cgcagttcgt tctctgaatc
      301 ccatgatgtg gtaaaaaatg aagtattata agactcatca ctataatttt gaattacatt
      361 cgcaagtctt atctgtttag gaacaaatga caactctaca ttcgtattat tttgatggtt
      421 taaattctta attgtttctc ttactgtttc gcttaatcca tagaatcttc caaagcttct
      481 acctgcttgt ccagaaccga tattagctcc taaa
//
LOCUS       DATXQK010000161          512 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.161, whole genome shotgun sequence.
ACCESSION   DATXQK010000161 DATXQK010000000
VERSION     DATXQK010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.161"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
BASE COUNT          221 a           77 c           88 g          126 t
ORIGIN      
        1 ttaaatgatg aagaaataag attaaacata atggaaattt tagaagaaag agtagatgat
       61 gatgtagaaa aatcacctaa agatgaaata acataattgt cccttggtta caagggcgca
      121 attatacacc ctaaagggtg cttgcgtgcc tttagagcaa aacccttgca gggagcaaca
      181 accattcgtt ttttaaaagt caagggtaaa taaactcgct aaggctcgcc attgactttt
      241 aaaatttgaa atgaacaaaa caattaagcc gacatactga aacaacaaaa attaattcag
      301 tagtcggctt tttctattct atcatttcaa aacgctcatt cacaaagagg atataaaaat
      361 ctattaagcc tccacctaaa acaacaaaaa aagaaaggaa gtgataaaaa atgacagaca
      421 gttttcattt ttcagtaaac ataatatcaa gaggaaaagg gaaaagtgca gtagcaagtg
      481 cagcatatat aagtggagaa aaaataaaaa at
//
LOCUS       DATXQK010000162          511 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.162, whole genome shotgun sequence.
ACCESSION   DATXQK010000162 DATXQK010000000
VERSION     DATXQK010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.162"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..>511
                     /locus_tag="VC394_001846"
     CDS             <1..>511
                     /locus_tag="VC394_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1-like element IS1548 family transposase"
                     /protein_id="HEP5995262.1"
                     /translation="VQFEQSLTSLDAVHQLISVDGKTIRGNRGKNQKPVHIVTAYDGG
                     HHLSLGQVAVEEKSNEIVAIPQLLRTIDIRKSIVTIDAMGTQTAIVDTIIKGKADYCL
                     AVKGNQETLYDDIALYFSDVNLLEELQENAQYYQTVEKSRGQIEVREYWVSSDIKWLC
                     QNHPKWHKLR"
BASE COUNT          169 a           82 c          118 g          142 t
ORIGIN      
        1 aagttcaatt tgagcaatca ttgacaagct tagatgccgt tcatcaactg atttcagtgg
       61 acggtaaaac gattcgaggc aatcgaggta aaaatcagaa gcctgttcat attgtaacgg
      121 cttatgatgg gggtcatcat cttagtttgg gacaggtagc ggttgaggag aaaagtaatg
      181 aaattgttgc cattcctcag ttattgcgga caattgatat ccgtaaaagc attgtaacga
      241 tagacgcaat gggcacgcag acggctatcg ttgatacgat tataaaaggt aaagcagact
      301 attgcttagc cgtcaaagga aatcaagaaa cactttatga tgatattgct ctttatttta
      361 gtgatgtcaa cttattggaa gaactccaag aaaatgcaca gtattatcag actgttgaaa
      421 aatctagggg acagattgaa gttagagaat actgggtgtc ttccgatatc aaatggttgt
      481 gtcaaaacca tcccaaatgg cataagttac g
//
LOCUS       DATXQK010000163          504 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.163, whole genome shotgun sequence.
ACCESSION   DATXQK010000163 DATXQK010000000
VERSION     DATXQK010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.163"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..>504)
                     /locus_tag="VC394_001847"
     CDS             complement(<1..>504)
                     /locus_tag="VC394_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002990276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEP5995263.1"
                     /translation="AIELMTTLGEVALGSQTIFNILFMPAFVMNLLILFFRPHITQMA
                     IALIRGQIKEFNKIQVQLFAYLGVFSLIALVGSGLFGIPFLSILYGTNLTDYWVDFML
                     IMLGGSIGSFATVIDNILTAMRKQQLLLIPYTGGFLISLLITNLFVMKYHILGAALSF
                     LITMLVW"
BASE COUNT          207 a           92 c           67 g          138 t
ORIGIN      
        1 accaaaccaa cattgttatt aaaaagctca aagcagctcc taaaatatga tatttcataa
       61 caaaaagatt ggtaattaat aacgaaatca aaaatcctcc tgtatatgga ataagaagaa
      121 gttgctgttt tctcatagcg gttaagatat tatcgataac tgtcgcaaaa ctaccaatcg
      181 agcctcctag cataatcaac ataaaatcta cccaataatc cgtaaggtta gtgccatata
      241 gtattgataa aaagggaata ccaaacaaac cacttccaac taacgctatc agtgaaaaaa
      301 ctcctaaata agcaaacaat tgaacttgta tcttattaaa ctcttttatt tgacctctga
      361 ttaaagcaat agccatctgt gtaatatgag gtctaaaaaa taaaattagc aaattcataa
      421 caaaggctgg cataaataaa atattaaaaa ttgtttgaga gcccaaagca acttcaccta
      481 aagtcgtcat tagttcaata gcgt
//
LOCUS       DATXQK010000164          503 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.164, whole genome shotgun sequence.
ACCESSION   DATXQK010000164 DATXQK010000000
VERSION     DATXQK010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.164"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..497
                     /locus_tag="VC394_001848"
     CDS             <1..497
                     /locus_tag="VC394_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HEP5995264.1"
                     /translation="SRDVDLKKVEYARVLLIALINLIISMILSLMLIVISFVLPTPSL
                     ISIGRILLTILLIWLTTLWQIPFILWLSRKINVYFAMIINIISPLIIGTSFSLLNKWY
                     LFPYDWSLKLLEPMTRMRINSIPFGAEFVPDYSQIFISLFLGIAFFILLTNLFAISFK
                     KQVK"
BASE COUNT          152 a           71 c           81 g          199 t
ORIGIN      
        1 tatcaaggga tgttgattta aaaaaggtag aatatgccag agttctattg atagctctta
       61 taaatctaat aattagtatg attttaagct taatgcttat tgtgattagt tttgtcctac
      121 ctactccaag tcttattagt ataggtagga tactattaac tatcttgtta atttggttga
      181 ctacactatg gcaaatcccg tttattttat ggctatcaag aaaaattaat gtgtattttg
      241 ctatgattat taatattata tctccactaa ttattggtac aagtttttct ctcttaaata
      301 aatggtattt gttcccttat gattggtcgt tgaagttgct tgagccaatg acaagaatga
      361 gaataaatag tatacctttt ggagcggagt ttgttccaga ctactcacag atttttatat
      421 cattgttcct aggaattgct tttttcatct tactgaccaa tctatttgct atctctttca
      481 aaaaacaggt gaaatagtca tga
//
LOCUS       DATXQK010000165          492 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.165, whole genome shotgun sequence.
ACCESSION   DATXQK010000165 DATXQK010000000
VERSION     DATXQK010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.165"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..201
                     /locus_tag="VC394_001849"
     CDS             <1..201
                     /locus_tag="VC394_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="HEP5995265.1"
                     /translation="QEKKILKQDIEKRRKERTHRLITRGAILESLIENAEELTDEEIK
                     ILLEEAIKTKEFKETLKLMREN"
BASE COUNT          197 a           97 c           95 g          103 t
ORIGIN      
        1 caagaaaaaa agatattaaa acaagacata gaaaaaagaa gaaaagaaag aacacatagg
       61 ctcataacaa gaggagcaat cttagaaagc cttatagaaa atgcagaaga actaacagat
      121 gaagaaataa aaatcctact agaagaagca ataaagacaa aagaatttaa agaaacacta
      181 aaactaatga gagaaaatta ataaaaataa agggtgttgt aaataatcaa atctccctta
      241 gattttctaa gggcgcaatt atacagctat cagagattga tgcattgcgt tctccgaagg
      301 ctcctaatcg gagggctgtg atttgtgcga tcaatgagcc aaacggatat acttgcatat
      361 tcactcgccg aatgccccaa cagccgacag aatgtcggct tccgcagtca tggtctgctc
      421 caaatcaaac acggacgcta cgcttcccct gcgctggctg ccgccagcca ccctttgaaa
      481 ggaaaaattt tt
//
LOCUS       DATXQK010000166          468 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.166, whole genome shotgun sequence.
ACCESSION   DATXQK010000166 DATXQK010000000
VERSION     DATXQK010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.166"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..457)
                     /locus_tag="VC394_001850"
     CDS             complement(<1..457)
                     /locus_tag="VC394_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_115157939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-like surface protein"
                     /protein_id="HEP5995266.1"
                     /translation="MFRRSKNNSYDTSQTKQRFSIKKFKFGAASVLIGLSFLGGVTQG
                     NLNIFEESIVAASTIPGSAATLNTSITKNIQNGNAYIDLYDVKNGLIDPQNLIVLNPS
                     SYSANYYIKQGAKYYSNPSEITTTGSATITFNILDETGNPHKKADGQIDI"
BASE COUNT          144 a           81 c           68 g          175 t
ORIGIN      
        1 ctatatcaat ttgtccatca gcttttttat gtggatttcc agtttcatca agtatattaa
       61 aagtaatagt tgctgaacca gttgttgtaa tttcactcgg attactataa tatttagcac
      121 cttgtttgat ataataattt gctgaatagc ttgatggatt taatacaatg aggttttgag
      181 gatcaatcaa tccattcttt acatcatata aatctatgta agcatttccg ttttgtatat
      241 ttttagtgat gcttgtattt aaggtcgctg cactccctgg aattgtagat gcagcaacta
      301 ttgactcttc aaaaatatta agattacctt gtgtaacccc acccaaaaaa ctaagaccaa
      361 ttagtacaga agctgcacca aacttgaact tcttaattga aaaccgttgt ttcgtctgtg
      421 aagtatcata actgttattt ttagaccttc taaacataat attctcct
//
LOCUS       DATXQK010000167          437 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.167, whole genome shotgun sequence.
ACCESSION   DATXQK010000167 DATXQK010000000
VERSION     DATXQK010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.167"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..>437)
                     /locus_tag="VC394_001851"
     CDS             complement(<1..>437)
                     /locus_tag="VC394_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009880288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase 2 family protein"
                     /protein_id="HEP5995267.1"
                     /translation="TDGTPQIFLETKTYNTKVSIVSEAKGKGYNVRNIFEYAINHVPN
                     FSGLILIDGDVVSMKKMWLEKMFIAIESGNDLIIPNYARKSFEGNATNHFIYPMLVKF
                     FKRDMPYQCISGDFGFSRGLIKDLTLKCNWHKYTLGYGIDIFL"
BASE COUNT          148 a           80 c           54 g          155 t
ORIGIN      
        1 taaaaaaata tctatcccgt aacctaatgt atatttgtgc caattacact tcaaagttaa
       61 atcttttatc aatcctcttg aaaatccaaa atcaccagag atgcattgat aaggcatatc
      121 acgtttgaaa aatttcacta acatgggata aataaaatgg tttgtcgcat ttccttcaaa
      181 agattttcta gcataatttg ggattataag atcatttcca gattcaattg ctataaacat
      241 cttttctaac cacatttttt tcattgagac aacgtctcca tcaatcaaaa ttaaaccgga
      301 gaaattcggc acatgattta ttgcatattc gaaaatattt ctcacattat aaccttttcc
      361 tttagcttct gatactatcg aaactttggt gttataagtt ttggtttcta aaaaaatctg
      421 cggagttcca tctgtac
//
LOCUS       DATXQK010000168          418 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.168, whole genome shotgun sequence.
ACCESSION   DATXQK010000168 DATXQK010000000
VERSION     DATXQK010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..418
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.168"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
BASE COUNT          149 a           56 c           75 g          138 t
ORIGIN      
        1 gaaatttttt aaaaattaaa taattttatt aaaggagaat agtataaaaa aagatttaat
       61 agcaaagtga taactttgct tgctgtaagc tctttaagtt tgggatttac ggccgtaact
      121 agtgttgtca gtgcggcaac agcacatcca gctccatctc caattattga tgaatggaat
      181 tatggagtaa gtgacaacta tggttattct aattattatg ttaaatctcc aaacaatatt
      241 ggttcaaaat cttcgataac taattattgg ggatccatta aggcatctga ttctcaaaaa
      301 atacgggtgg gcactgtcaa gttcaaaaaa agcttggaat gatattcggt tgaatgcaca
      361 ttggaattac tttaattttt agaaatggta tatgcaagct aaagacccaa taattatt
//
LOCUS       DATXQK010000169          415 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.169, whole genome shotgun sequence.
ACCESSION   DATXQK010000169 DATXQK010000000
VERSION     DATXQK010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.169"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
BASE COUNT          142 a           48 c           82 g          143 t
ORIGIN      
        1 tagatgccag tttggatttt tgatatttgt gttgagttta aaagataaaa acacttaatg
       61 aaaatctgtt ttttcttaga aaggggacca gtatgattga gacgctaatg agtacgttgg
      121 aaatggttag aaaaaaaatt ctagtaggac tgcaatcgaa gatgactttg aggaatataa
      181 aaagagaaat ctaccaatct ttgtcagtga agatgatagt gtgatctatt atagactagg
      241 gttcattttt aatgaattct atctcaatat ttcaggctgg agacaagaac taaagactaa
      301 gcttactagt atttctttaa tttctttaca ttttattggt gttgttgacg aacaaaaaaa
      361 gggggaatat ctaggttctt tttctgatac atctgaattc ttaagtagac atcca
//
LOCUS       DATXQK010000170          406 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.170, whole genome shotgun sequence.
ACCESSION   DATXQK010000170 DATXQK010000000
VERSION     DATXQK010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.170"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
BASE COUNT          146 a           59 c           74 g          127 t
ORIGIN      
        1 taaagaggtt tcatgactta ttttagatta aaaaaagaat taaactgatc actaagaaat
       61 aaaggaattg tagtttacga agggaatcaa aaacttcaga tagccattaa tacaggtaaa
      121 aataacagtg aaaaaagtct tttttgatac tttttttgag gtggttcggg cggcagcgac
      181 tttctccaca ggaaaaaaca tctgttttgg tcttggctta cttcgttcgc agaaatagga
      241 aatcgggtat cacattagca gagtagttca tgttttttga gcatcacact ggatctgtct
      301 acgttctaaa agctttcttc atttttgtat cataccccct acaagtagaa aaagtataac
      361 aaagaaaatt tataaacaga aatggctagt attatcaagg aattta
//
LOCUS       DATXQK010000171          371 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.171, whole genome shotgun sequence.
ACCESSION   DATXQK010000171 DATXQK010000000
VERSION     DATXQK010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.171"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..>371)
                     /locus_tag="VC394_001852"
     CDS             complement(<1..>371)
                     /locus_tag="VC394_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HEP5995268.1"
                     /translation="ILMYSSLYKVTKDERYLTFSNIYVEKLVDIISRDGIESPSLYAG
                     TAGIALAIKEASISGKYYPKLLSSLNCLLKEQIKDKLVVSFSNIEKGIIEPYDYDMVN
                     GFSGITNYLLLEQEFFSVELN"
BASE COUNT          125 a           62 c           49 g          135 t
ORIGIN      
        1 gatttaactc tacagaaaaa aattcttgtt caaggagtaa gtaattagtt attccactaa
       61 atccgttaac catatcataa tcatatggct caataattcc tttctcaata ttagaaaaac
      121 ttactaccaa tttgtcttta atttgttctt ttagaagaca atttaaggat gataataatt
      181 tcggataata ctttccagat atactcgctt ctttaatagc taatgcaata cctgcagtgc
      241 cagcatagag agacggagat tctataccat ctctagagat tatgtctact aatttctcaa
      301 cgtatatgtt ggagaatgtt agatatctct catcttttgt cactttatat agtgaactat
      361 acattaagat t
//
LOCUS       DATXQK010000172          351 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.172, whole genome shotgun sequence.
ACCESSION   DATXQK010000172 DATXQK010000000
VERSION     DATXQK010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.172"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            122..>351
                     /locus_tag="VC394_001853"
     CDS             122..>351
                     /locus_tag="VC394_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011054996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5995269.1"
                     /translation="MTLIDTAFIWLATILRQKQGDIFKRIISIISLTIWQYLVSVLTR
                     GTPLFLSSGLQIILLYCYTVEITNLILYGHKQ"
BASE COUNT          109 a           59 c           56 g          127 t
ORIGIN      
        1 aacaaagagt aagttctttg ggactgcaat aatcttgttg caacaattaa tagccttgtt
       61 aattttggtc tataatcgag agaacctgtc tcttcttttc tctgaaaaag tagctcttgt
      121 aatgaccctt attgatacag cttttatttg gctggcaaca atattacgcc aaaaacaagg
      181 tgatattttt aaaaggatta tttctatcat ctccttaaca atttggcaat acttagtatc
      241 tgtgctgaca agaggaactc ctctcttttt aagcagtgga ctacagatta tattgctcta
      301 ttgttatacc gttgagatta ctaatttaat attatatggg cacaaacaat t
//
LOCUS       DATXQK010000173          349 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.173, whole genome shotgun sequence.
ACCESSION   DATXQK010000173 DATXQK010000000
VERSION     DATXQK010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.173"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..>349
                     /locus_tag="VC394_001854"
     CDS             <1..>349
                     /locus_tag="VC394_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012560530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase"
                     /protein_id="HEP5995270.1"
                     /translation="YLTTTGSCIDFGEEGAVGRGNKTHGIISSFRPNTMEAPAGKNCT
                     YFVGKVWGFLSDTIAKEIYEAFNTPCQIIMQLNIGSKLYRPTHLFIQTEESVDQERVL
                     EIVNRHLNNGKQNT"
BASE COUNT          127 a           45 c           65 g          112 t
ORIGIN      
        1 ttatttaact actacaggtt cttgtattga tttcggagaa gaaggtgcag ttggtcgagg
       61 taataaaact catggtatca tatcaagttt taggccaaat actatggaag caccagctgg
      121 aaaaaattgt acttattttg taggaaaggt ttggggattt ttgtctgata ctattgccaa
      181 agaaatttat gaagcattca atactccctg tcaaatcatt atgcaattaa atattggatc
      241 aaaattgtat agaccaaccc atttatttat tcagacagaa gaaagtgttg atcaagaaag
      301 agtattagaa attgttaaca ggcatttaaa taatggtaaa caaaatact
//
LOCUS       DATXQK010000174          345 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.174, whole genome shotgun sequence.
ACCESSION   DATXQK010000174 DATXQK010000000
VERSION     DATXQK010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.174"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            complement(<1..>345)
                     /locus_tag="VC394_001855"
     CDS             complement(<1..>345)
                     /locus_tag="VC394_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiopeptide-type bacteriocin biosynthesis
                     protein"
                     /protein_id="HEP5995271.1"
                     /translation="DWLFFKFYGPKERQEELLNNWKKFWLNLPSELFSQSNMFYMRYN
                     DTNDHIRIRINCDSIENNFSLYLSVVQDLLPQLIESCIISDIEVSSYKPEVNRYGGPH
                     LISYAEEIFCKES"
BASE COUNT          113 a           60 c           44 g          128 t
ORIGIN      
        1 gattctttac aaaaaatttc ctctgcataa cttattaaat gaggtcctcc atatctattt
       61 acttctggtt tatagctact cacttctatg tcagagataa tacaactctc aatcagttga
      121 ggtaatagat cttgaactac tgacaaatac aaagaaaagt tattttcaat cgaatcacaa
      181 tttattcgaa ttcttatatg atcattggta tcattatatc tcatataaaa catgttactt
      241 tggctaaaca gttctgatgg caggtttagc caaaattttt tccagttatt tagtaattct
      301 tcttgtcgct cttttggacc ataaaatttg aaaaatagcc agtca
//
LOCUS       DATXQK010000175          329 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.175, whole genome shotgun sequence.
ACCESSION   DATXQK010000175 DATXQK010000000
VERSION     DATXQK010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..329
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.175"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            157..>329
                     /locus_tag="VC394_001856"
     CDS             157..>329
                     /locus_tag="VC394_001856"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEP5995272.1"
                     /translation="MKDNKELMKDKKVATNRRSIIFISVPKSSIITTNLLEPEVGINE
                     KLAKNIAVTIAEN"
BASE COUNT          137 a           48 c           45 g           99 t
ORIGIN      
        1 tatttatttt tgaaattatt ataagtttca actaacaaaa ttaatgttct tagcacaaag
       61 aacgaactta ggaacatcat tactgttgag ctcaaccaac ttgaaactac taaaattgaa
      121 attaaatcag tatcagtctt actaaagaat agagaaatga aagataataa agaactaatg
      181 aaagataaaa aagtagcaac taataggcgg tccattattt ttatctctgt ccctaaatct
      241 tccatcatca ctactaattt attagaaccg gaagttggta tcaatgaaaa actagcaaag
      301 aatattgcgg ttacaattgc tgaaaatga
//
LOCUS       DATXQK010000176          325 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.176, whole genome shotgun sequence.
ACCESSION   DATXQK010000176 DATXQK010000000
VERSION     DATXQK010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.176"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            36..>325
                     /locus_tag="VC394_001857"
     CDS             36..>325
                     /locus_tag="VC394_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEP5995273.1"
                     /translation="MATYRQRGKKKLWDYRVYGKDGKLIASGSGFRTKKEASYEALKI
                     EQKKYYSDVINSNATLFEMWQIWYDLVVEPSELDILTKQKYQARGKIIEKYLS"
BASE COUNT          136 a           33 c           63 g           93 t
ORIGIN      
        1 aggataaatt ccttgatgtc taggaaagga gaaaaatggc aacatataga caaagaggta
       61 aaaagaaatt atgggattat cgtgtttatg gtaaagatgg aaaattaata gcatctggtt
      121 caggctttag aacaaaaaaa gaagcatcat atgaagcttt aaaaattgag caaaaaaaat
      181 attattcaga tgttataaat tccaatgcta cattatttga gatgtggcaa atatggtatg
      241 atcttgttgt tgagccaagt gaattagata ttcttactaa gcaaaaatat caagcccgag
      301 gaaagataat tgaaaaatat ttatc
//
LOCUS       DATXQK010000177          325 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.177, whole genome shotgun sequence.
ACCESSION   DATXQK010000177 DATXQK010000000
VERSION     DATXQK010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.177"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            18..173
                     /locus_tag="VC394_001858"
     CDS             18..173
                     /locus_tag="VC394_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A2 lantipeptide"
                     /protein_id="HEP5995274.1"
                     /translation="MSFMKNSKDILTNAIEEVSEKELMEVAGGKKGSGWFATITDDCP
                     NSVFVCC"
BASE COUNT          120 a           36 c           65 g          104 t
ORIGIN      
        1 tactgataga aaggagaatg agttttatga aaaattcaaa ggatattttg actaatgcta
       61 tcgaagaagt ttctgaaaaa gaacttatgg aagtagctgg tggtaaaaaa ggttccggtt
      121 ggtttgcaac tattactgat gactgtccga actcagtatt cgtttgttgt taattaaaac
      181 aatctggata agcagactaa aatttcctgt ttagacttag tctaggcagg atttttaagt
      241 agtggtagga aaaaatgata aaaagaaatg aattaaagtt agaatatcag caacatcagt
      301 tttacgaata ctttaactca atttt
//
LOCUS       DATXQK010000178          313 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.178, whole genome shotgun sequence.
ACCESSION   DATXQK010000178 DATXQK010000000
VERSION     DATXQK010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.178"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..>313
                     /gene="spej"
                     /locus_tag="VC394_001859"
     CDS             <1..>313
                     /gene="spej"
                     /locus_tag="VC394_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="streptococcal pyrogenic exotoxin SpeJ"
                     /protein_id="HEP5995275.1"
                     /translation="SVNSNSENSKIVGNLLIDGVQQKTLINPIKIDKRIFTIQEFDFK
                     IRQYLMQTYKIYDPNSPYIKGQLEIAINGNKHESFNLYDATSSSTRSDIFKKYKDNKT
                     I"
BASE COUNT          141 a           45 c           40 g           87 t
ORIGIN      
        1 catcagtaaa cagtaattcg gaaaatagta aaattgtagg taatttacta atagatggag
       61 tccagcaaaa aacactaata aatcccataa aaatagataa acgtattttt acgattcaag
      121 aatttgactt caaaatcaga caatatctta tgcaaacata caaaatttat gatcctaatt
      181 ctccatacat aaaagggcaa ttagaaattg cgatcaatgg caataaacat gaaagtttta
      241 acttatacga tgcaacctca tctagtacaa ggagtgatat ttttaaaaaa tataaagaca
      301 ataagactat aaa
//
LOCUS       DATXQK010000179          307 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.179, whole genome shotgun sequence.
ACCESSION   DATXQK010000179 DATXQK010000000
VERSION     DATXQK010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.179"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
BASE COUNT          123 a           54 c           41 g           89 t
ORIGIN      
        1 agtcttgatt ccaactttta aatattcgta tatgaagtaa agctactgtg catgaaacaa
       61 aaaatataac aataagtgga acaattgcca ttttctgttg gttatataga gtatctgata
      121 ttcttaaaag ccatgcccat ggaataagaa accatacctt atcaccaagg ctcgtttcac
      181 aaaaagctgc aacaataaca cctaccattg aaatacctaa tgccacacct gtactaaata
      241 tatagctaat aatatgattg atcagatagg ttacataggc aaaaatgata taaattaaac
      301 tattcaa
//
LOCUS       DATXQK010000180          302 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.180, whole genome shotgun sequence.
ACCESSION   DATXQK010000180 DATXQK010000000
VERSION     DATXQK010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.180"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
BASE COUNT          121 a           44 c           40 g           97 t
ORIGIN      
        1 aaaaaggtca aagactttct gacaccaaat aaaaatagaa ttttttctaa ctaaaaataa
       61 tagcacaatc tttggcagca atctgaattg tttttccata aaagatttaa gcacttacgt
      121 ttgcttaaaa tataggttat gtgacgtgcg tgcaagatca gattataaca aaatagttcc
      181 aaataagcct atcactgaga aagcatcaca gaagggttaa actttttttg tattagtgtt
      241 ttatcagtac aacctaacac ttatttttat aaaaaaataa aaaagctatt gacaatctta
      301 ta
//
LOCUS       DATXQK010000181          289 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.181, whole genome shotgun sequence.
ACCESSION   DATXQK010000181 DATXQK010000000
VERSION     DATXQK010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.181"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..>289
                     /locus_tag="VC394_001860"
     CDS             <1..>289
                     /locus_tag="VC394_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011528611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HEP5995276.1"
                     /translation="YSKRAAFRTTPFGIFTAINTVDLTKGTTSNVQKVSFIKKAVPDY
                     SWIYSLVKSYEISNLEKLSLKINTAAFTQGDRYVLPFTVNESEEDRNISFS"
BASE COUNT          110 a           42 c           47 g           90 t
ORIGIN      
        1 gttattctaa aagagcagcg tttagaacta ctccatttgg tatctttact gctattaata
       61 cagttgattt gacaaaggga accacttcta atgtacaaaa ggttagtttt attaaaaaag
      121 cagtcccaga ttattcatgg atatattctc tagtaaaatc gtatgaaata agtaaccttg
      181 aaaaactttc attaaaaata aatacagcgg cgtttactca aggtgacaga tatgtactac
      241 catttacagt gaatgaatca gaggaagata gaaacataag tttttcaaa
//
LOCUS       DATXQK010000182          263 bp    DNA     linear   BCT 28-DEC-2023
DEFINITION  TPA_asm: Streptococcus pyogenes strain MGAS28351
            SAMN08556264-rid19676293.denovo.182, whole genome shotgun sequence.
ACCESSION   DATXQK010000182 DATXQK010000000
VERSION     DATXQK010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN08556264
            Sequence Read Archive: SRR6740612
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pyogenes
  ORGANISM  Streptococcus pyogenes
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-OCT-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-OCT-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001918171.2
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001918171.2
            Genome Coverage       :: 270x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/12/2023 10:18:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 1,861
            CDSs (total)                      :: 1,818
            Genes (coding)                    :: 1,771
            CDSs (with protein)               :: 1,771
            Genes (RNA)                       :: 43
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1 (5S)
            tRNAs                             :: 36
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 47
            CDSs (without protein)            :: 47
            Pseudo Genes (ambiguous residues) :: 0 of 47
            Pseudo Genes (frameshifted)       :: 28 of 47
            Pseudo Genes (incomplete)         :: 23 of 47
            Pseudo Genes (internal stop)      :: 8 of 47
            Pseudo Genes (multiple problems)  :: 11 of 47
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Streptococcus pyogenes"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN08556264-rid19676293.denovo.182"
                     /strain="MGAS28351"
                     /serotype="Emm28"
                     /isolation_source="normally sterile site"
                     /host="Homo sapiens"
                     /db_xref="taxon:1314"
                     /country="USA: California"
                     /collection_date="1999"
                     /collected_by="CDC Active Bacterial Core Surveillance"
     gene            <1..>263
                     /locus_tag="VC394_001861"
     CDS             <1..>263
                     /locus_tag="VC394_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEP5995277.1"
                     /translation="KKAERIPLEGRSKAQRATEIIQALRNQFPLEMLLEILDLSRSTY
                     YYQVKRLAQGDKDIELKHVIREIYDEHKGNYGYRRIHMELRNRG"
BASE COUNT           94 a           44 c           59 g           66 t
ORIGIN      
        1 aaaaaagctg agagaatacc gcttgaggga cgaagtaaag ctcaaagagc aacagaaatc
       61 attcaagcat taaggaatca atttccctta gagatgctac ttgagatcct tgatttatca
      121 cgctccacct attactatca agtgaagcga ctagctcaag gagataagga tatagaacta
      181 aagcatgtga ttcgagagat ttatgatgag cataaaggta attacggtta tcgtcgtatt
      241 catatggagt tgcgtaaccg agg
//
